LOCUS       DAJVQR010000001        95305 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVQR010000001 DAJVQR010000000
VERSION     DAJVQR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OQM52_000001"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OQM52_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1562017.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OQM52_000002"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OQM52_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1562018.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OQM52_000003"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OQM52_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1562019.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OQM52_000004"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OQM52_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1562020.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OQM52_000005"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OQM52_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1562021.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OQM52_000006"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OQM52_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1562022.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OQM52_000007"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OQM52_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1562023.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OQM52_000008"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OQM52_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1562024.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OQM52_000009"
     CDS             complement(8942..9223)
                     /locus_tag="OQM52_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562025.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OQM52_000010"
     CDS             complement(9237..10898)
                     /locus_tag="OQM52_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1562026.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OQM52_000011"
     CDS             complement(10882..11208)
                     /locus_tag="OQM52_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1562027.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OQM52_000012"
     CDS             complement(11264..11446)
                     /locus_tag="OQM52_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562028.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OQM52_000013"
     CDS             complement(11430..11870)
                     /locus_tag="OQM52_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1562029.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OQM52_000014"
     CDS             complement(11870..12166)
                     /locus_tag="OQM52_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1562030.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="OQM52_000015"
     CDS             complement(12163..12501)
                     /locus_tag="OQM52_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1562031.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OQM52_000016"
     CDS             complement(12498..13709)
                     /locus_tag="OQM52_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1562032.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OQM52_000017"
     CDS             complement(13711..14283)
                     /locus_tag="OQM52_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1562033.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OQM52_000018"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OQM52_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="OQM52_000019"
     CDS             complement(15781..16005)
                     /locus_tag="OQM52_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562034.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16147..16308)
                     /locus_tag="OQM52_000020"
     CDS             complement(16147..16308)
                     /locus_tag="OQM52_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1562035.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16318..16623)
                     /locus_tag="OQM52_000021"
     CDS             complement(16318..16623)
                     /locus_tag="OQM52_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562036.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="OQM52_000022"
     CDS             complement(16620..16898)
                     /locus_tag="OQM52_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562037.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="OQM52_000023"
     CDS             complement(17187..18935)
                     /locus_tag="OQM52_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1562038.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="OQM52_000024"
     CDS             complement(18932..19231)
                     /locus_tag="OQM52_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1562039.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="OQM52_000025"
     CDS             complement(19237..19470)
                     /locus_tag="OQM52_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562040.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="OQM52_000026"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="OQM52_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="OQM52_000027"
     CDS             complement(19685..19906)
                     /locus_tag="OQM52_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562041.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="OQM52_000028"
     CDS             complement(19896..20096)
                     /locus_tag="OQM52_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1562042.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="OQM52_000029"
     CDS             20226..20405
                     /locus_tag="OQM52_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562043.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="OQM52_000030"
     CDS             20530..20859
                     /locus_tag="OQM52_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562044.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="OQM52_000031"
     CDS             complement(20909..21097)
                     /locus_tag="OQM52_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1562045.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="OQM52_000032"
     CDS             complement(21472..22701)
                     /locus_tag="OQM52_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1562046.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22950..24071
                     /gene="roxA"
                     /locus_tag="OQM52_000033"
                     /gene_synonym="ycfD"
     CDS             22950..24071
                     /gene="roxA"
                     /locus_tag="OQM52_000033"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1562047.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OQM52_000034"
     CDS             complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OQM52_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1562048.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25596..26732
                     /gene="potA"
                     /locus_tag="OQM52_000035"
     CDS             25596..26732
                     /gene="potA"
                     /locus_tag="OQM52_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1562049.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26716..27573
                     /gene="potB"
                     /locus_tag="OQM52_000036"
     CDS             26716..27573
                     /gene="potB"
                     /locus_tag="OQM52_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1562050.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27570..28364
                     /gene="potC"
                     /locus_tag="OQM52_000037"
     CDS             27570..28364
                     /gene="potC"
                     /locus_tag="OQM52_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1562051.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28361..29407
                     /gene="potD"
                     /locus_tag="OQM52_000038"
     CDS             28361..29407
                     /gene="potD"
                     /locus_tag="OQM52_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1562052.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OQM52_000039"
     CDS             complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OQM52_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1562053.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OQM52_000040"
     CDS             complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OQM52_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1562054.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OQM52_000041"
     CDS             complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OQM52_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1562055.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OQM52_000042"
     CDS             complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OQM52_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1562056.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OQM52_000043"
     CDS             complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OQM52_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1562057.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34739..35812
                     /locus_tag="OQM52_000044"
     CDS             34739..35812
                     /locus_tag="OQM52_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1562058.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35940..39386
                     /gene="mfd"
                     /locus_tag="OQM52_000045"
     CDS             35940..39386
                     /gene="mfd"
                     /locus_tag="OQM52_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1562059.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39529..40491
                     /gene="ldtC"
                     /locus_tag="OQM52_000046"
     CDS             39529..40491
                     /gene="ldtC"
                     /locus_tag="OQM52_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCS1562060.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40574..40786)
                     /locus_tag="OQM52_000047"
     CDS             complement(40574..40786)
                     /locus_tag="OQM52_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1562061.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41071..41703
                     /gene="comR"
                     /locus_tag="OQM52_000048"
     CDS             41071..41703
                     /gene="comR"
                     /locus_tag="OQM52_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1562062.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41765..42304)
                     /locus_tag="OQM52_000049"
     CDS             complement(41765..42304)
                     /locus_tag="OQM52_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1562063.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OQM52_000050"
     CDS             complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OQM52_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1562064.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44243..44785)
                     /locus_tag="OQM52_000051"
     CDS             complement(44243..44785)
                     /locus_tag="OQM52_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1562065.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OQM52_000052"
     CDS             complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OQM52_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1562066.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OQM52_000053"
     CDS             complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OQM52_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1562067.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OQM52_000054"
     CDS             complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OQM52_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1562068.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47305..47682)
                     /locus_tag="OQM52_000055"
     CDS             complement(47305..47682)
                     /locus_tag="OQM52_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1562069.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OQM52_000056"
     CDS             complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OQM52_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1562070.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48377..50562
                     /gene="fhuE"
                     /locus_tag="OQM52_000057"
                     /pseudo
     CDS             48377..50562
                     /gene="fhuE"
                     /locus_tag="OQM52_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50622..52055)
                     /gene="ptsG"
                     /locus_tag="OQM52_000058"
     CDS             complement(50622..52055)
                     /gene="ptsG"
                     /locus_tag="OQM52_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1562071.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52350..53147)
                     /locus_tag="OQM52_000059"
     CDS             complement(52350..53147)
                     /locus_tag="OQM52_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1562072.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53158..54162)
                     /gene="holB"
                     /locus_tag="OQM52_000060"
     CDS             complement(53158..54162)
                     /gene="holB"
                     /locus_tag="OQM52_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1562073.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54159..54800)
                     /locus_tag="OQM52_000061"
     CDS             complement(54159..54800)
                     /locus_tag="OQM52_000061"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1562074.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54790..55812)
                     /gene="yceG"
                     /locus_tag="OQM52_000062"
     CDS             complement(54790..55812)
                     /gene="yceG"
                     /locus_tag="OQM52_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1562075.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55815..56624)
                     /gene="pabC"
                     /locus_tag="OQM52_000063"
     CDS             complement(55815..56624)
                     /gene="pabC"
                     /locus_tag="OQM52_000063"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1562076.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56744..57985)
                     /gene="fabF"
                     /locus_tag="OQM52_000064"
     CDS             complement(56744..57985)
                     /gene="fabF"
                     /locus_tag="OQM52_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1562077.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58072..58308)
                     /gene="acpP"
                     /locus_tag="OQM52_000065"
     CDS             complement(58072..58308)
                     /gene="acpP"
                     /locus_tag="OQM52_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1562078.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58519..59253)
                     /gene="fabG"
                     /locus_tag="OQM52_000066"
     CDS             complement(58519..59253)
                     /gene="fabG"
                     /locus_tag="OQM52_000066"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1562079.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59266..60195)
                     /gene="fabD"
                     /locus_tag="OQM52_000067"
     CDS             complement(59266..60195)
                     /gene="fabD"
                     /locus_tag="OQM52_000067"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1562080.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60211..61164)
                     /gene="fabH"
                     /locus_tag="OQM52_000068"
     CDS             complement(60211..61164)
                     /gene="fabH"
                     /locus_tag="OQM52_000068"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1562081.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61232..62302)
                     /gene="plsX"
                     /locus_tag="OQM52_000069"
     CDS             complement(61232..62302)
                     /gene="plsX"
                     /locus_tag="OQM52_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1562082.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62383..62556)
                     /gene="rpmF"
                     /locus_tag="OQM52_000070"
     CDS             complement(62383..62556)
                     /gene="rpmF"
                     /locus_tag="OQM52_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1562083.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62608..63129)
                     /gene="yceD"
                     /locus_tag="OQM52_000071"
     CDS             complement(62608..63129)
                     /gene="yceD"
                     /locus_tag="OQM52_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1562084.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63328..63912
                     /gene="yceF"
                     /locus_tag="OQM52_000072"
     CDS             63328..63912
                     /gene="yceF"
                     /locus_tag="OQM52_000072"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1562085.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64024..64983)
                     /gene="rluC"
                     /locus_tag="OQM52_000073"
     CDS             complement(64024..64983)
                     /gene="rluC"
                     /locus_tag="OQM52_000073"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1562086.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65556..68747
                     /gene="rne"
                     /locus_tag="OQM52_000074"
     CDS             65556..68747
                     /gene="rne"
                     /locus_tag="OQM52_000074"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1562087.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68944..69886)
                     /gene="flgL"
                     /locus_tag="OQM52_000075"
                     /pseudo
     CDS             complement(68944..69886)
                     /gene="flgL"
                     /locus_tag="OQM52_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69898..71540)
                     /gene="flgK"
                     /locus_tag="OQM52_000076"
                     /pseudo
     CDS             complement(69898..71540)
                     /gene="flgK"
                     /locus_tag="OQM52_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71606..72547)
                     /gene="flgJ"
                     /locus_tag="OQM52_000077"
     CDS             complement(71606..72547)
                     /gene="flgJ"
                     /locus_tag="OQM52_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS1562088.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72547..73644)
                     /gene="flgI"
                     /locus_tag="OQM52_000078"
     CDS             complement(72547..73644)
                     /gene="flgI"
                     /locus_tag="OQM52_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1562089.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73656..74354)
                     /gene="flgH"
                     /locus_tag="OQM52_000079"
     CDS             complement(73656..74354)
                     /gene="flgH"
                     /locus_tag="OQM52_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1562090.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74407..75189)
                     /gene="flgG"
                     /locus_tag="OQM52_000080"
     CDS             complement(74407..75189)
                     /gene="flgG"
                     /locus_tag="OQM52_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCS1562091.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75327..76078)
                     /gene="flgF"
                     /locus_tag="OQM52_000081"
                     /pseudo
     CDS             complement(75327..76078)
                     /gene="flgF"
                     /locus_tag="OQM52_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76098..77237)
                     /locus_tag="OQM52_000082"
     CDS             complement(76098..77237)
                     /locus_tag="OQM52_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1562092.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(77262..77957)
                     /gene="flgD"
                     /locus_tag="OQM52_000083"
     CDS             complement(77262..77957)
                     /gene="flgD"
                     /locus_tag="OQM52_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1562093.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77969..78372)
                     /gene="flgC"
                     /locus_tag="OQM52_000084"
                     /pseudo
     CDS             complement(77969..78372)
                     /gene="flgC"
                     /locus_tag="OQM52_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78376..78792)
                     /gene="flgB"
                     /locus_tag="OQM52_000085"
     CDS             complement(78376..78792)
                     /gene="flgB"
                     /locus_tag="OQM52_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1562094.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78947..79606
                     /gene="flgA"
                     /locus_tag="OQM52_000086"
     CDS             78947..79606
                     /gene="flgA"
                     /locus_tag="OQM52_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1562095.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79682..79975
                     /gene="flgM"
                     /locus_tag="OQM52_000087"
     CDS             79682..79975
                     /gene="flgM"
                     /locus_tag="OQM52_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1562096.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79980..80396
                     /gene="flgN"
                     /locus_tag="OQM52_000088"
     CDS             79980..80396
                     /gene="flgN"
                     /locus_tag="OQM52_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1562097.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80436..81971)
                     /gene="murJ"
                     /locus_tag="OQM52_000089"
     CDS             complement(80436..81971)
                     /gene="murJ"
                     /locus_tag="OQM52_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1562098.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82171..83004)
                     /locus_tag="OQM52_000090"
     CDS             complement(82171..83004)
                     /locus_tag="OQM52_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1562099.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83006..83653)
                     /locus_tag="OQM52_000091"
     CDS             complement(83006..83653)
                     /locus_tag="OQM52_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1562100.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83664..84248)
                     /gene="rimJ"
                     /locus_tag="OQM52_000092"
     CDS             complement(83664..84248)
                     /gene="rimJ"
                     /locus_tag="OQM52_000092"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1562101.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84484..85692
                     /gene="mdtH"
                     /locus_tag="OQM52_000093"
     CDS             84484..85692
                     /gene="mdtH"
                     /locus_tag="OQM52_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1562102.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85756..86403
                     /gene="grxB"
                     /locus_tag="OQM52_000094"
     CDS             85756..86403
                     /gene="grxB"
                     /locus_tag="OQM52_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1562103.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86537..87097
                     /locus_tag="OQM52_000095"
     CDS             86537..87097
                     /locus_tag="OQM52_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1562104.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87203..88249
                     /gene="pyrC"
                     /locus_tag="OQM52_000096"
     CDS             87203..88249
                     /gene="pyrC"
                     /locus_tag="OQM52_000096"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1562105.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88323..88568
                     /gene="dinI"
                     /locus_tag="OQM52_000097"
     CDS             88323..88568
                     /gene="dinI"
                     /locus_tag="OQM52_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1562106.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88858..89112
                     /gene="bssS"
                     /locus_tag="OQM52_000098"
     CDS             88858..89112
                     /gene="bssS"
                     /locus_tag="OQM52_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1562107.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89227..90345
                     /gene="solA"
                     /locus_tag="OQM52_000099"
     CDS             89227..90345
                     /gene="solA"
                     /locus_tag="OQM52_000099"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1562108.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90393..90506
                     /locus_tag="OQM52_000100"
     CDS             90393..90506
                     /locus_tag="OQM52_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1562109.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90767..91333
                     /locus_tag="OQM52_000101"
     CDS             90767..91333
                     /locus_tag="OQM52_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1562110.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91337..91912
                     /locus_tag="OQM52_000102"
     CDS             91337..91912
                     /locus_tag="OQM52_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1562111.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91954..93006)
                     /locus_tag="OQM52_000103"
     CDS             complement(91954..93006)
                     /locus_tag="OQM52_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1562112.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93231..94151
                     /gene="lpxL"
                     /locus_tag="OQM52_000104"
     CDS             93231..94151
                     /gene="lpxL"
                     /locus_tag="OQM52_000104"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1562113.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94323..94520
                     /locus_tag="OQM52_000105"
     CDS             94323..94520
                     /locus_tag="OQM52_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1562114.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94544..>95305
                     /locus_tag="OQM52_000106"
     CDS             94544..>95305
                     /locus_tag="OQM52_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1562115.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTP"
BASE COUNT        22925 a        25266 c        23514 g        23600 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22921 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22981 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    23041 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
    23101 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
    23161 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg
    23221 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    23281 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    23341 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    23401 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    23461 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
    23521 cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa
    23581 ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    23641 cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    23701 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    23761 ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag
    23821 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
    23881 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23941 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    24001 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    24061 tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct
    24121 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    24181 ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    24241 gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc
    24301 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
    24361 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag
    24421 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    24481 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    24541 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
    24601 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    24661 cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg
    24721 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    24781 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    24841 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    24901 atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta
    24961 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    25021 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    25081 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    25141 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    25201 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    25261 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    25321 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    25381 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    25441 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    25501 ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt ggttaaccac
    25561 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    25621 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    25681 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    25741 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    25801 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    25861 gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa
    25921 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25981 tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct
    26041 ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag cctcgtctgt
    26101 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    26161 agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg
    26221 aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag
    26281 acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg
    26341 gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg
    26401 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    26461 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    26521 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    26581 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    26641 atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    26701 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    26761 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    26821 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa ctacacgcgt
    26881 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26941 accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac
    27001 aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt
    27061 cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    27121 tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc
    27181 ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    27241 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    27301 atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg
    27361 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    27421 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc
    27481 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    27541 cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    27601 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    27661 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    27721 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    27781 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    27841 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga
    27901 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27961 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
    28021 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    28081 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    28141 taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt
    28201 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    28261 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    28321 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    28381 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    28441 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    28501 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    28561 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    28621 cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    28681 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
    28741 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    28801 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
    28861 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28921 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    28981 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    29041 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
    29101 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    29161 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    29221 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
    29281 agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    29341 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    29401 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    29461 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt
    29521 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa
    29581 cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc
    29641 attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc
    29701 ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac
    29761 atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    29821 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    29881 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    29941 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    30001 cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc
    30061 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    30121 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    30181 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    30241 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    30301 aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa
    30361 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    30421 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gtggaacacg
    30481 agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa
    30541 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
    30601 cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    30661 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata
    30721 cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc
    30781 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
    30841 atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag
    30901 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    30961 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    31021 atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc
    31081 ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc
    31141 tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc
    31201 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    31261 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    31321 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    31381 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    31441 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg
    31501 agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg
    31561 aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat
    31621 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    31681 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    31741 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    31801 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    31861 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    31921 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    31981 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    32041 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    32101 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    32161 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    32221 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac
    32281 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca
    32341 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca
    32401 ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    32461 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    32521 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    32581 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    32641 ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc
    32701 ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    32761 gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
    32821 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    32881 cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt
    32941 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    33001 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag
    33061 tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc
    33121 taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc
    33181 accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg
    33241 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    33301 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    33361 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc
    33421 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    33481 caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga
    33541 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    33601 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca
    33661 ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    33721 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    33781 caactctcag cggctcatcc agccacaaac gccagccggt aatattggct gccggataac
    33841 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    33901 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
    33961 tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact
    34021 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    34081 tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca
    34141 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    34201 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    34261 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    34321 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    34381 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt
    34441 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    34501 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    34561 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat tttatcatca
    34621 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    34681 tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat
    34741 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    34801 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    34861 agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt
    34921 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    34981 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    35041 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    35101 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
    35161 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    35221 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    35281 tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg
    35341 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    35401 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    35461 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    35521 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    35581 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    35641 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    35701 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    35761 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac
    35821 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    35881 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    35941 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    36001 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    36061 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    36121 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    36181 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    36241 tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    36301 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    36361 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    36421 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    36481 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    36541 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    36601 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    36661 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc
    36721 agccattgtt cttcagcgaa ccactgccac cgctgttcag ttatttccct gccaatacct
    36781 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    36841 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    36901 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa
    36961 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    37021 tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    37081 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    37141 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    37201 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc
    37261 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    37321 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    37381 agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag
    37441 ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg
    37501 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    37561 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    37621 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    37681 tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa
    37741 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    37801 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    37861 ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc
    37921 ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    37981 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    38041 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    38101 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    38161 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    38221 gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    38281 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    38341 cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa
    38401 acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg
    38461 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    38521 gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca
    38581 acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg
    38641 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    38701 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    38761 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    38821 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    38881 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    38941 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga
    39001 acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    39061 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    39121 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    39181 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    39241 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    39301 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    39361 aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc
    39421 aacatttaca aattctttgc actttcctgc actatccgac actgtcacca tccataattc
    39481 aggcttatct gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa
    39541 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    39601 ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac
    39661 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    39721 gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg
    39781 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    39841 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    39901 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    39961 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    40021 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    40081 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    40141 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    40201 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    40261 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    40321 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    40381 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    40441 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    40501 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    40561 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    40621 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    40681 ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt
    40741 ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg
    40801 ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    40861 gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    40921 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt caaatagaaa
    40981 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    41041 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    41101 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    41161 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga
    41221 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    41281 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    41341 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    41401 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    41461 ggagatatag ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    41521 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    41581 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    41641 ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    41701 taagtcgcaa aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    41761 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    41821 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    41881 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    41941 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    42001 cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc
    42061 caccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    42121 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    42181 gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca
    42241 ctgccgctac gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat
    42301 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    42361 ctatcagaca gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag
    42421 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    42481 ttgtccaggg cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac
    42541 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    42601 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    42661 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    42721 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc
    42781 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    42841 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    42901 gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata
    42961 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    43021 cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    43081 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    43141 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    43201 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc
    43261 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    43321 ttcactttgc catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc
    43381 tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct
    43441 ggcgtattga aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt
    43501 ttacgttccg gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt
    43561 ttcgcttcac gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc
    43621 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    43681 tgcagcagtg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    43741 ttcttacgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    43801 ccgacaatca caatcttttt caatggcgta gtcaacgtgc cccccttaaa tttattaacc
    43861 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    43921 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    43981 atcaagtttt gttgcttaaa agataggcaa ctttaccggg ggagatcaaa aaatagagtt
    44041 tattgtttat tatttgagca aattcggaag ggtaatatag ggtgaagcgt atatcgaggg
    44101 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    44161 atgcggcgtg gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
    44221 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
    44281 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
    44341 gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa
    44401 ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
    44461 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    44521 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    44581 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
    44641 ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    44701 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    44761 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctact
    44821 ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga
    44881 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    44941 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    45001 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
    45061 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaccccagt
    45121 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
    45181 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    45241 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    45301 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    45361 attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga
    45421 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
    45481 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
    45541 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
    45601 aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc
    45661 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    45721 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    45781 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    45841 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    45901 atcgccggtt tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg
    45961 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    46021 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
    46081 cgcgatatca ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg
    46141 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc
    46201 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc
    46261 gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg
    46321 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    46381 taccgcaccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    46441 tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    46501 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
    46561 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    46621 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    46681 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    46741 tgccagaggc gcaggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
    46801 ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    46861 acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
    46921 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    46981 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    47041 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt
    47101 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    47161 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    47221 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    47281 tttttgtcat caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc
    47341 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg
    47401 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg
    47461 aacggtaacg ggttcttgcc tttcgttata aatcgttgag gatgctgaag gttgaatatc
    47521 agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat
    47581 caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag
    47641 taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc
    47701 gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct
    47761 tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct
    47821 tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct
    47881 gacacgtcgt tcacagtcgg aatgagaata ttcggaatga tcagaatatg cgttggcgct
    47941 tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg
    48001 atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc
    48061 aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg
    48121 agttaaacct gctctttcaa tcttaactca cgatttctta agcaaaaaat cgttttgctg
    48181 ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg
    48241 tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttgc agcaaaatta
    48301 ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata
    48361 aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagtcat
    48421 caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt ctgccgcttt
    48481 tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga
    48541 tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca
    48601 acatgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt
    48661 acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc
    48721 cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg ttgatggtat
    48781 ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata tggcactgtt
    48841 tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc
    48901 tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag gcgatgtctc
    48961 ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac
    49021 cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct
    49081 ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg atttaggcga
    49141 tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata gcccaacctg
    49201 gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc gcgcacgcag
    49261 taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa
    49321 gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg aagtcgaatt
    49381 tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg
    49441 gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt ggaacagtgg
    49501 caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat tgtttggtcg
    49561 tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt cagttcatgg
    49621 gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa
    49681 gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa atcgttatat
    49741 gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc acgttatacc
    49801 aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac gccttacgct
    49861 ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc
    49921 cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac
    49981 tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc
    50041 ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac
    50101 ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga atttgaactc
    50161 aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta tcttgcagag
    50221 gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc
    50281 agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa ctggcaaaat
    50341 cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca aggtagctac
    50401 gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac
    50461 gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat cgtctacggc
    50521 gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag
    50581 gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac
    50641 tcatccatct cggttttcag gttatcggat ttagtaccga aaatagcctg aacaccagaa
    50701 ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc cactttagac
    50761 acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatgttttct
    50821 ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt tgcttttgca
    50881 tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg
    50941 aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag ccacagtttg
    51001 ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga cgtaccgtca
    51061 cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg
    51121 tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat accggtcagg
    51181 aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta
    51241 gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca
    51301 ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa
    51361 acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac gttccagatg
    51421 tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact
    51481 accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc
    51541 caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca
    51601 aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta
    51661 aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt atccgccaag
    51721 tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg
    51781 gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg
    51841 gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg
    51901 cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg
    51961 acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag cgatttaccg
    52021 accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatggg
    52081 tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt acagggcatc
    52141 taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg tttgacggct
    52201 atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac atatgtcaca
    52261 atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac tcgttttagc
    52321 cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg ggaagcgtcg
    52381 atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc aacggcaaca
    52441 cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg attctctttt
    52501 cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa tcgatccagg
    52561 gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac aatgccggaa
    52621 aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc ctctgtaaaa
    52681 cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc cagcgtatcg
    52741 gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc aatctggata
    52801 tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata ataatccagc
    52861 ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa atcttctaca
    52921 tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac gttgtcacgt
    52981 tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac tgccagacaa
    53041 aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat
    53101 tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat gtctctctta
    53161 aagatgagga accggtaaca caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag
    53221 atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt cacgaatgtg
    53281 gcaaacatcc cccagtatag cctgcaggca cgagggagaa agatggtttg ccagttcggc
    53341 gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc gttttagcgc
    53401 atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat gattaagggc
    53461 cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct gacacaatgt
    53521 ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag gcgaaccggc
    53581 gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca
    53641 agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac gactacgtaa
    53701 tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg
    53761 ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt
    53821 tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt cggtgacctc
    53881 acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg tgtagtaatc
    53941 gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac ttttgtggcc
    54001 ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat catcgcccat
    54061 gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat agctggctac
    54121 cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa ctccttcacc
    54181 cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg ggtggcatca
    54241 atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg ggtgcgatta
    54301 aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt
    54361 aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa atccccgaga
    54421 acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc gccctgatac
    54481 gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc cagcgcaggt
    54541 ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac ttcggcttta
    54601 tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct taacttttcg
    54661 gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc cagttgctcg
    54721 agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag cccctcaatg
    54781 acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct gcacagactt
    54841 gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg ccacaaaata
    54901 gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc ccggcgtagc
    54961 tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg gcgtttccag
    55021 gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca cggttgggtc
    55081 ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa cctgatcgcg
    55141 ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca gctgattttt
    55201 atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat
    55261 tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat acatcaaggt
    55321 gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg cctgcgctac
    55381 ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac gcaattgctt
    55441 gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg cttctttacc
    55501 gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc
    55561 ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa cccgtgggcg
    55621 attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg tccctggctt
    55681 cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat ggcgaacctt
    55741 ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga taatcaataa
    55801 cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc atataacgtt
    55861 gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat taacgcatta
    55921 caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac ttcgacaagc
    55981 tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc gttaacacct
    56041 gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa attagccgca
    56101 cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc
    56161 tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat acctgcaaga
    56221 tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc gttacgcaaa
    56281 cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg tccgctgttc
    56341 aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag cacaccattt
    56401 tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa gtcacaggaa
    56461 atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa caaactgact
    56521 ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt tgcccgatcg
    56581 cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc tccttatcag
    56641 agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa gctaagaaaa
    56701 aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc tttttaaaga
    56761 tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat tccattccgc
    56821 tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc gggttatcca
    56881 ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag atagattcta
    56941 ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt accaacacac
    57001 ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta tcgccagccg
    57061 gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg cctgcatcac
    57121 gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt gacgtcatat
    57181 gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt
    57241 ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc agtacgaagc
    57301 catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg cgggtagata
    57361 atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc tctgcgccac
    57421 ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg ccaatgttgt
    57481 gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg ccatacatga
    57541 tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat gggctgatct
    57601 tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg aggccgccaa
    57661 tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt tcaaggccag
    57721 aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca tccatcttgc
    57781 gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag ccagcaaatt
    57841 tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc tgaccggcaa
    57901 gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg cccagtccgg
    57961 tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat gattctagtg
    58021 ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg
    58081 gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc
    58141 cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga
    58201 atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc
    58261 cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac tcttaaattt
    58321 cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga aaaatttgca
    58381 actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta ccccccaata
    58441 acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg caacatgagc
    58501 attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca aagtttcacc
    58561 cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga tttcctgtgc
    58621 gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt catcgctcag
    58681 cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag taataccgcg
    58741 tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt tcgccgcagc
    58801 gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag tgataatacg
    58861 accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga aaacagatga
    58921 aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta acaggttatc
    58981 acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg cgcgaatttt
    59041 ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac ctttgccgtt
    59101 ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag tgccaataac
    59161 tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc
    59221 ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa gctcgagcgc
    59281 cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt
    59341 cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta cgccttgcgc
    59401 tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac gtaccagtgc
    59461 gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca caacagaaac
    59521 tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt
    59581 catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt ttgcgccagc
    59641 agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa taaccacctg
    59701 tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt cttcacacgc
    59761 tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg tgccttccgg
    59821 tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc
    59881 aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg ccatcattgc
    59941 cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg cagtcaacag
    60001 cgctggctgg gtttgccagg ttttattcag ttcttcagct gacccctgct gggtcagcgc
    60061 ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga caattggata
    60121 gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag ggaacacaaa
    60181 tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag agccccaagt
    60241 gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc gcccgtcgcg
    60301 tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt ggcgatccag
    60361 cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac tgataatacg
    60421 caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat tattcgccgc
    60481 cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga cttcgttgcc
    60541 cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg gcagcgtcag
    60601 caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg gctcttcaga
    60661 ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc cacgatctgt
    60721 tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact tcaccgcccc
    60781 agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac aggctgctgc
    60841 aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac aagctgcgct
    60901 cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct ggtctttctc
    60961 aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc
    61021 gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc actcgtcaga
    61081 ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca gatagctgcc
    61141 agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata taccgtcact
    61201 tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt ttgccaccgt
    61261 ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt cgctgaggaa
    61321 cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct cgctgattgg
    61381 ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg ccgttatact
    61441 ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa cgctttaata
    61501 acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga gaaaggaaca
    61561 tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg tcacaaacca
    61621 gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata taattgatag
    61681 aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc tttacttctt
    61741 cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc tcttcagcca
    61801 gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg acgttggccc
    61861 ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc accagcgccg
    61921 gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc agtgccccgg
    61981 tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc agggccatac
    62041 gcattgaact cccacggctg gcgcggatag cttgcgaagg ccgggcatca ctggcgataa
    62101 ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct ttagcaagta
    62161 atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa ttagaattca
    62221 gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc atgacatcta
    62281 acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc
    62341 ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag cgatgacctt
    62401 gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt caccagaagt
    62461 tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac gcatgccacg
    62521 tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc aattacggtt
    62581 tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta atacggcaaa
    62641 tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg cttcggacac
    62701 ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga tttcatcttc
    62761 aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc
    62821 cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg ttgtgtagac
    62881 ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca ccttcgcatc
    62941 gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc attccacatc
    63001 actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg tatagatacc
    63061 ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg gtaattttac
    63121 cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga aaaaggcagc
    63181 ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt attttcgttc
    63241 agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc
    63301 aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc cacctcgcca
    63361 tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc acctgaggtc
    63421 gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa
    63481 aaatcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc agaccaggta
    63541 tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa
    63601 ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt gtttaattcg
    63661 gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg tcacctaagc
    63721 gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc gggtagcttt
    63781 aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga ccctaacacg
    63841 ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg
    63901 ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat cggtgcaggc
    63961 ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc ttatatgctt
    64021 gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat ccatcggcgc
    64081 ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg cggcgtgcag
    64141 gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt cacggtcacc
    64201 gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac ggatctgatg
    64261 agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc gttcttccac
    64321 tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac gttcgccgct
    64381 ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact gccactgacc
    64441 gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct catgcagaga
    64501 acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc ggtcgagacg
    64561 atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt cgataacgcc
    64621 gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca gcaccaggat
    64681 atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca gatgtggcga
    64741 aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct catcacccgc
    64801 ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac ctttacgcaa
    64861 aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa agttatcgat
    64921 acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag tctctgtttt
    64981 catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc tatgcttatt
    65041 tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt attaaaccaa
    65101 tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt
    65161 gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac
    65221 aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa
    65281 gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg
    65341 aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca
    65401 tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc
    65461 gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc
    65521 ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa
    65581 ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat gacctggata
    65641 tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca
    65701 ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc
    65761 cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca
    65821 acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc
    65881 gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc
    65941 tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc gatgaccgta
    66001 ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg cttatcgtgc
    66061 gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgcctga
    66121 aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc ctgattcatc
    66181 aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat atcggcgaaa
    66241 tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct gcattaggtc
    66301 gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg ttcagccact
    66361 accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg ccatccggtg
    66421 gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac tccgcacgcg
    66481 cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa gccgccgatg
    66541 agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg
    66601 acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc
    66661 aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc
    66721 gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg cgctgctccg
    66781 gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt ttgatcgaag
    66841 aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg ccaatcgctt
    66901 cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt caggacggtg
    66961 ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac gtgttgcgcg
    67021 tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg catgaagaag
    67081 cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa caacctgcac
    67141 tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa cctgccgcga
    67201 ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct gcacaacctg
    67261 ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt gaagaagcca
    67321 aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc
    67381 gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac
    67441 gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc
    67501 aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa gcacgtacca
    67561 ccgacgagca gcaagtgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc
    67621 aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac
    67681 aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag
    67741 tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt gaagaaactg
    67801 ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg gtgaaagtcc
    67861 cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc
    67921 gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg
    67981 gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca atgccgttga
    68041 ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg
    68101 tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta caagtgcagc
    68161 cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc gcgccagttg
    68221 ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg caaccggaag
    68281 tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag ccgcaggtga
    68341 ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa ccgatggttg
    68401 aaccgcagga agagacggca gatgcagata ttgaagaagt tgccgaaact gctgaggttg
    68461 tggttgctga acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag
    68521 cagaagttga aacggtggcc gcggttgaac cagagatcac cgttgagcat aaccacgcta
    68581 ccgcgccaat gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg
    68641 actggcagcg ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa
    68701 cgcatcatgc ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa
    68761 gtaatcaagc cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa
    68821 aattcatcaa atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc
    68881 cccggcgatc ccagagcggg cagacaaaac atacccagtt tcatgatatg ttttaaagcg
    68941 agcttatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg ctttatacga
    69001 tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc agtccacatc
    69061 aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta aggaatccag
    69121 cgactccagt ttgttcagct gcgtgcctaa ttccgcgcga acggtaagga cattattcag
    69181 cgagtttttc agtccacggt tggttttatc caatgccgca gcggcgattt ctttatccgc
    69241 ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg ctatccagca tggcaaaaag
    69301 attggtttca gaagcgctac cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa
    69361 aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat
    69421 actttctgtt ccgccaacat aatcaccgtt agcctcacta aacggcgcag tctctgtttt
    69481 atatgtagcg cccattaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc
    69541 cctgaatatc cgtagccagc gaggcccggt catcgtcact caaggtgcca ttgctggcgt
    69601 agacaatttt ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt
    69661 ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct
    69721 gtgcctggga gagaacaaca gcttgtgatg cagcaatggg atcgtctgaa gggttaacga
    69781 ctcgcttacc cgtcgacatc tgctcgccgt acttcatcca ttctgcctga gaattggtga
    69841 tgccgcgcat gttttgctgg tacatcatct gggtactgaa acgcatttta tctcccctta
    69901 gcgaatgtta atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc
    69961 caggtaatac tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga
    70021 aatcgactgc tgctgattgg aaagctgcgt caccacatta ccttgcgtgg tgctactggt
    70081 tttcaacgtc gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa
    70141 ggatttcgca ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt
    70201 gcggttgtcg ctatcacccg catcttcttc gctcgccatc gcgattttcg cttcgtcggt
    70261 gattaataca tccatgttga cgatggcgtc acttactggt ttcagcgtga agctgtcgtt
    70321 aacggcaggc gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc
    70381 atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg gtgacctgcc actgattatt
    70441 atcgaacgag attttgtaat ctgtcgccag taccacggag gcatcagtta ccgtggcacc
    70501 gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa
    70561 gaaatcttca ccagcatcgc cgttagcatc aaatccggct ttgtgttggc tgttgaaagc
    70621 ctcggcaaat gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga
    70681 acggaatgtc agaatgccgc ccagcgaccc ggtattcagt aatttctccg ggatctcaat
    70741 attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg
    70801 aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggcatcgtg
    70861 atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg gtttaattcg
    70921 ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc ccccacgcct
    70981 gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt gttgatctga
    71041 tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag atattgatcg
    71101 gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg gcgcgccgcc
    71161 gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg catctgtgtt
    71221 gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg ctcatagcgg
    71281 gcggtcaggc cgctactttg cgtctgcgca gcacgtaact ggttggtaat aaacgtatca
    71341 tactcacgct gcacaccaga aacgtagacg ccattgccaa cccagccgcc agcgcccaac
    71401 gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac gttatagctg
    71461 gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc gctcatggcg
    71521 ttattgatca agctggacat ggaggttcct tataagcctt caattacaga gtattatcgg
    71581 cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg ctgtaggttt
    71641 tgctcacctt gtcgcttatc gatttcatct gctggatcat gttggtgagt ttacgggcat
    71701 agtgaggatc ggtagcatag cccgcgtcct gtaggacctg cgccccctgt tccgcactcg
    71761 cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg taatccgaca
    71821 atgcttccag atacgagctg tagacccgaa acttcgcttt tactttcttc gcttcgccgt
    71881 tttcatattc agtcgtggtg atttcagtga cttgcccttt ccagttgcca gaggctttga
    71941 caccaaacag gttatagctt ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac
    72001 cagattcaag cgcagcctga gcgaggatca aatgatgtgg cacaccgctt tgctggcttg
    72061 ccagttgggc gggcagcgag agttgcgcga ggaatgctct actgtcaccc ggcagcgaat
    72121 catcgtagtt acgtggtacg gccttttgca ccagctgcga aagcgtctgg ttttgataac
    72181 gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg cgtggactcc tctggcaatg
    72241 gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct ttgcccgccg
    72301 tcatctgttg ggcaatttgc tggtcataca tactggtata cagacgagtg tgctcgctgc
    72361 tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca
    72421 tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg ctcgctttcg
    72481 cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc
    72541 tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca ttgattgcaa
    72601 aatagacatc agatccatcg gcgtagcgcc cagcgcattg agcgcacgca ccacgttatt
    72661 gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta aatcgatctg
    72721 cgtttgtgga gtaacaacag tctgtccacc accaaacggt gtatctggct ggctgacatt
    72781 ggcctgacga ttaactgtta ctgagagatt tccctgcgct accgcgcagc tgtcgagggt
    72841 cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt tagcgtcctg
    72901 cggggtgaca ttaacctgca tattctggat atcggcaagg aaacggacct gggaactgtt
    72961 gccacttggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc tgccatatcc
    73021 acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag
    73081 ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt caataaccgc
    73141 accattggtg atccgtccac cgttcagttg gttaacctga acactgctac cgcctgcgga
    73201 ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct gactgtcaac
    73261 gcccttaagc ggagtcatca acaacgtacc tccacgcaag cttttggcat tacccatgga
    73321 agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg acgctgtcac
    73381 cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg taattcccag
    73441 ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc
    73501 agtgccatcc agccccacta ccaagccata gccaatcagt gagttttgcc ttaccccctg
    73561 aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat
    73621 taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag gttaaggaag
    73681 aaacgctgca accagcccat attttgcgct tcgttaatgt agccattgcc tacgtattca
    73741 atacgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat agtgcgtgga
    73801 ttaaccacgc ccgagaagcg aataaattcg gtaccctgat taatggcaat ctgtttttca
    73861 cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt caacgtgccg
    73921 ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc accggaggct
    73981 tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac agtatcaaag
    74041 ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct ggcgctgacg
    74101 ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac
    74161 agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc gttggcgacg
    74221 ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt
    74281 atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata agtatgcgca
    74341 gcgttttttt gcatggctat catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt
    74401 tatgccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca ccgctttact
    74461 gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg ccacgttgac
    74521 gttagacgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc ccggcgtgct
    74581 ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa tgctctccag
    74641 tccggtatca ttcataaagg tggtgagatt gagctgccca acctgaaccg gagctgcctg
    74701 gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta acgcattcgc
    74761 cggaatggtg atcgctggct gcacctgaaa accaccagcc gtcaccagct gcccgttctg
    74821 atccacctgg aaagagccgt cacggatata ggctgatgag ccatctggca acattacctg
    74881 gaaaaagccc tgccctttaa tcgcgacatc tttgctgtta ttggtctgcg acagattgcc
    74941 ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga
    75001 gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt gataaagcag
    75061 atcttcaaac accgcgcgct gacgcttaaa accgttagta ctgacgtttg ccaggttgtt
    75121 ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga tccataatga
    75181 actgatcata taatgtcctg tagtggattg ctgacaagca gagcagaagc acgcgacagt
    75241 gtgaatttcc cctcacccta accctctccc caaaggggcg agggaatagt ccgcgcgctt
    75301 ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca gcagttggtt ggcacggcct
    75361 gcgttatcat cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca
    75421 atcatgtcgc tcattgccgc aacggcattg acgttactgc cttccagaac ccccgacatc
    75481 acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg tggcctgggt ttctgcgctt
    75541 aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc ccgtggcttt caccagtttc
    75601 agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg gattgagcgc cgagattgtg
    75661 ccatcggcag caatcgtgat ttccgcccct tccggcacag caattggccc cgcctcgcct
    75721 atcaccggat gcccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta
    75781 cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca gccatcctgc tgcaacgcaa
    75841 cgtccagcgg gcgagaggtg taatccatct tgccgggcgt catgtctgca cccggtgttg
    75901 acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc caccggcact gcgcgcaagg
    75961 cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc cagattactg gcggttaccg
    76021 cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg tgatccattg
    76081 agctatcccg tcagcgatta gcgtaagtta acccgcgtgt tgaggatctg gtcctgggtt
    76141 ttgatggtct gggcgttaga cttatagtta cgctgggcaa cgatcatatt gaccagttct
    76201 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagagtgcc aaagtttccc
    76261 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    76321 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    76381 tgggtttgtt cgttggaatt gttgccgaca accgtaccgt catcattgat ttgataactc
    76441 accagatcgc ccggtttgta gccattctgg gtggttgcca caatgttgtt agcgccggtt
    76501 gcgatattat tcgccatcgc accatccact aatgtgccat tagcattaaa ttccagtgtt
    76561 gtcgctgtct tcgcaatgct gtttggatca ctgctatcct gggtgtagac ctgccagtta
    76621 ttatccccgg tcttcacaaa atagacgctc atgtcatgag cattaccctg actgtcgaaa
    76681 acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa tggcgtaaca
    76741 gtaggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt ggtagttttc
    76801 gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg ctgaatagtc
    76861 ggcggcgtac cggttaccgg gtaacccgtc agctgtaaac cttgcgtatt caacaggtta
    76921 cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc gttgctgtct
    76981 accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc ggtgttggtg
    77041 gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag tcccactttc
    77101 gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta ggtggcggag
    77161 ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc
    77221 gcttgagaaa aggccatgac tgactcctga aatgtgaagg cttaaattat ctgccgtact
    77281 tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct gttgccgcgg
    77341 atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt accaccgtta
    77401 ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccatc agtcaacgaa
    77461 ccgtcccagg tgaaactgtg aactccggcg gtcagttcac caatatcaat ggtgcgcaca
    77521 accgcgccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg ttgcagctca
    77581 acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc cgcaagaaca
    77641 gttgtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa cgactggctg
    77701 ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat cccgctgacc
    77761 gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc
    77821 tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc tgcggcgttg
    77881 ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg atcggtggtg
    77941 gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt ttcagcatca
    78001 tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac gccgacatgg
    78061 tgttaaccat ctccccgacg acatcaacgt tcggcatttt tatgtagccc tttgcatctg
    78121 ccagcggatt acctggttca taaaccagtt tgtccggggc ctgactttct taacatcggc
    78181 aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata ccacctgttt
    78241 tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca gattactggc
    78301 cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat caaaaatatt
    78361 cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt tgatttgccc
    78421 gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact gggtgcgttc
    78481 gcgatccata tcgacggtat taccgtcaag cgaaggctgg tccggaatac ggtattgcag
    78541 ttctgcggta ggaggcgtca gcgcctgcgc cgggatgtgc tgcgttgagg tcatcgtcag
    78601 tgcaaccaca ctggttgcat cccgtccacg ttgcatgact tttttaagtt cactggcaaa
    78661 atcgatatcg cgcgcctgat aaccaggggt atcggcattg gcgatgtttg ctgccagcac
    78721 ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag
    78781 cttatcgagc atatctcctc cgcaggtatc aaaattctgc catcagctta aatgccttta
    78841 cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct atttatgccg ttgatggtca
    78901 ttgcggacag gtacaattca cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa
    78961 acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta agtacggcga gcaatctcac
    79021 atcgcaattg cacaactttt ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt
    79081 ttctattcgt acagcgccca atctactacc gccatgcgag cagccattgc tttcgatgag
    79141 caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg
    79201 atatttacag gttaatgtgc aggccacagg aaattatgtg gttgccgcga tgcctattgt
    79261 gcggggagga aagctggaag ctggcaatgt caaactgaaa cgtggacgcc tggataccct
    79321 gccaccgcgt acggtgctgg atatcaatca acttgttgat gccgttagcc tgcgcgatct
    79381 atcacccgat caacctatcc agttaaccca gtttcgccag gcatggcggg taaaagcggg
    79441 acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc gccaacgcag aaggtcaggc
    79501 gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atggtatcgg aacaggtagt
    79561 cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg ttaaagatta
    79621 cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg aggaataaac
    79681 catgagtatt gatcgcactt cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac
    79741 caccgacgcg ccggtaacga acagccgggc ggcaaaaaca accgcctcca ccagcaccag
    79801 tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg atatcaatct
    79861 tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa tggacaccgg
    79921 caaaattgcc gatgcgctga tcaacgaagc gcagcaagac ttgcagagta actgatcgta
    79981 tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg
    80041 taatggatca agagcagcaa catctctcta tggggcagat caacggcagc cagttgcaat
    80101 ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa cagttacgca
    80161 agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag gaaattactg
    80221 tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg gaaggacaga
    80281 ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg
    80341 gggcgaacgg tcagacctca acaccccatc gcggcagtaa aaagatttcg atctgaatat
    80401 tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg ggcaaattct
    80461 ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc cgccagcacg
    80521 accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc cggcatgata
    80581 tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag acgcaacaga
    80641 aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg ccagtaaagc
    80701 agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc atgtttcaac
    80761 ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat ggcaattttc
    80821 actggcgttt taatgtcctg gcgggaataa aagccaggag ccaacacttt cactacaatc
    80881 aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac
    80941 gcggtaaatt taccgtactg gaacagcgaa acggttaacg gacctgaaag aatgcccaac
    81001 gcaaccgcac tcggcagtgc caacaggaaa caaagacgca acccccagtc catcaaacgg
    81061 ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgacggcag caaaatggtg
    81121 ccaagcgcca cgcccagcac accggaggga aactccatta agcggtcggc gtaatacatc
    81181 caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat taaggagatc
    81241 tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg cattgctcca
    81301 gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag gtgcggtagc
    81361 tgatacacca gctgcagaat gccgccgacc gtaacagccc acgccagcgc cagcaccggt
    81421 gggttaaagt acggtgcggc aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt
    81481 ggcgcaaacg ccggaatcga gaagcggttc cacgtattca gaatcgctcc caccagcgac
    81541 gccagggaga tcagcaagat atagggaaag gtaatcttta gtagctggct ggtcagggca
    81601 aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca tgatcaccca cggtgcggcg
    81661 agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga aacataagag
    81721 acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat attccgccag aatcggtaca
    81781 aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg aagtttaaaa
    81841 gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac aattgcgtct
    81901 cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt
    81961 aataaattca tcggtgttct aatccggacc cacgggcaaa acgcctgcaa ggcaggcgct
    82021 gaaagaggcg aatagtctac ctagattatg gtgaattgct accgccagat gttacagggt
    82081 ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca gcactgccta
    82141 ttcgccggcg gtttgcggaa ctgtctgatt ttacacgcat tcaatgaagt gccgcgcaca
    82201 gccgacaaat ccacgttgct caagagtgct ctgccaacca ggaatcggtt tattcactac
    82261 accctgcccg cgctcctcac gccattcgcg catatccgta atgtcgatga gcgcaccgtc
    82321 agtcacggcc tgcacggttt cacgctgact tccggcacgg cgatgcatac aagtggtgat
    82381 ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc atttcgccag cgtcgttagt
    82441 cagtagcgta ccgccatcca gagaggcttt gccgcccgac aaccacagcg cggtatccac
    82501 cacatgcaga taatcatcca gcaacgtgaa ataaagatcg tgtggcccga cgctattgct
    82561 acgatgttta tccattctta gcgaggctgc ggtggcgagt tgcgttttta actcaccgta
    82621 gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc gcgccgccag
    82681 ttccaccagc ctttcagcat cgcgcagatt ttctgccagc ggtttatcga cacagacatg
    82741 tacccccgca ttgagtaacg tactgaccac gtcaaagtgg ctggcggtgc tggagtgcac
    82801 aaaaaccgca tcacaactgg cggcaaggct ggataacgaa tcggcataag gaatgcgcca
    82861 gctgtcacaa attggcaggg ctttggcgcg cgtaggcgac caggctcctt gtaacgtcca
    82921 gtcagacgct gccgccagca ccggtaacca cgctttttgc gcaatgccgc ctaatcccac
    82981 tacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac gaatcaagac
    83041 gttgtttcag ttctgccact tcgatttcca gggcttcgac gcgggtctgt aaatcaccgt
    83101 caaccatgtt cgacatagcc gttaccgccg gctgatcttc aacctcacca ctgaaaagat
    83161 gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca aaaggaccat
    83221 cttcgcgatt cgccagttgt tccagcgtcg actccacttc cgccatatcg ctgaattcat
    83281 acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg ggcaccacgc aataacaacg
    83341 tggtgattaa cgccacttct gcggcgctca gtttcagatc gccaaattct gaattacaaa
    83401 aacgttgctc atatttggtg acccgattac caaaaccgct cactgtgcgt agataatgac
    83461 gtttgaccag attatccagc tgttcctgca cttcggattc actcagattc atgaccggtt
    83521 cacggttcgt tttctgatta caggccgtga ctacaccatt gactgagagc gggtattgct
    83581 ccggcgtggt cacctgcttt tccagcaagc aaccaattac gcgagcttcc agggcagtaa
    83641 gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt agttaatgcc
    83701 gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc atagccttct
    83761 ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg catataatta
    83821 gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc ggtcagggct
    83881 tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag atagcaggca
    83941 tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc caataatctc tttttcgtcc
    84001 gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt aatcatccct
    84061 agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg ctcccagggc
    84121 ttgaggaaat ggcgattctc tgcgtaataa tccgccagac gccaggcatc acgatcatgc
    84181 accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt actgcgatag
    84241 ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa gtattactat
    84301 acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat tttctgcatt attcacatat
    84361 ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatactgtcc ccgaagtgat
    84421 gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt cccgggaggg
    84481 gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat atttcctgct catcgataat
    84541 atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg cttcgttgat
    84601 caaatgggct gggccgccgt catggtcggt attgctctcg gtctacgcca gtttattcag
    84661 caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa accgatgatt
    84721 gttaccggta tgctgatgcg cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg
    84781 tggctattgt ggttttcatg cctgctctcg ggactcggcg gcacgttgtt tgatccgccg
    84841 cgttcggcgc tggtggtgaa actaatccgt ccacaacaac gtggtcgttt tttctcgctg
    84901 ttgatgatgc aggacagtgc cagtgcggtc attggcgcat tgttggggag ctggctgttg
    84961 caatacgact ttcgcctggt ctgcgccaca ggggccgttt tgtttgtgct atgtgcggcg
    85021 ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa
    85081 ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct atgtcctgac gctggcgggt
    85141 tactacatgc tggctgtaca agtgatgctg atgctgccaa ttatggtcaa cgacgtggct
    85201 ggcgcgccct ctgccgttaa atggatgtat gccattgaag cgtgtctgtc gttaacgttg
    85261 ctctacccta tcgcccgctg gagtgaaaag cattttcgtc tggaacaccg gttgatggct
    85321 gggctgttga taatgtcatt aagcatgatg ccggtgggca tggtcagcgg cctacaacaa
    85381 cttttcaccc tgatttgtct gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa
    85441 accttaagtg cttcgctggc ggacgcaaga gctcgcggca gctatatggg gtttagccgt
    85501 ctgggtctgg cgattggcgg cgctattggt tatatcggtg gtggctggct gtttgacctg
    85561 ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc tgggaattat tggcatcttc
    85621 actttccttg cgctgggttg gcagtttagt cagaaacgcg ccgcgcgtcg tttgctggaa
    85681 cgcgacgcct gatttgatac tcccccctga ctcctccata ctgaaagtga actgacagcc
    85741 aaatggagga gtcacgtgaa gctatacatt tacgatcact gcccttactg cctcaaagcc
    85801 cgcatgattt tcggcctgaa aaatatcccc gtcgaattac atgttctgct caacgacgac
    85861 gcagaaacac ccacccggat ggtcggtcaa aaacaggttc ccattctgca aaaagatgac
    85921 agccgctata tgccagaaag catggatatc gttcactatg tcgataaact cgacggcaaa
    85981 ccgttactga ccggcaaacg ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc
    86041 tacgtcaaca aactgctgtt gccgcgtttt gccaaatcgg catttgatga gttttctact
    86101 cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac
    86161 ctgctggccc actctgacgg tctgattaag aatatcagcg atgatttgcg tgcgctggac
    86221 aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt cggaagatga tattcagcta
    86281 tttccgctac tgcgtaatct gacgctggta gccggaatca actggccaag ccgcgttgct
    86341 aattaccgcg ataatatggc gaaacagaca caaatcaatt tgttatcatc aatggcgatt
    86401 taatcctgac ccgccgcggc agcctgtatt cgcgggttgc cgccgttttc agttttttct
    86461 gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt caggatattg acgcagattt
    86521 catgtcgagg actaccatga acaagttttt atttgccgct gcattgatcg tcagtggcct
    86581 gctcgttggc tgtaatcaac tcacccaata caccatcacc gaacaagaaa ttaaccagtc
    86641 gcttgcgaaa cataataatt tctcaaaaga tatcggttta cccggcgtgg ctgacgccca
    86701 tattgttctg acaaacttga ccagccaaat tggtcgcgaa gagccgaata aggttaccct
    86761 aaccggagac gccaatctgg acatgaactc cctgttcggt agtcagaaag cgaccatgaa
    86821 actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa ggtgcgatct tcctgaaaga
    86881 gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt
    86941 gcttccctat ttgaaccagg cattacgcaa ttactttaac cagcaacctg cttacgtcct
    87001 gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt
    87061 gaagccaggc gaaattgtca ttccgtttac ggattaatga cgagggcgca ttcgcgccct
    87121 ttatttttcg tgcaaaggaa aacgtttccg cttatccttt gtgtccggca aaaacatccc
    87181 ttcagccgga gcatagagat taatgactgc accatcccag gtattaaaga tccgccgccc
    87241 agacgactgg caccttcacc tccgcgatgg cgacatgtta aaaactgtcg tgccgtatac
    87301 cagcgaaatt tatggacggg ctatcgtaat gcccaatctg gctccgcccg tgaccacagt
    87361 tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc gtacctgccg ggcacgattt
    87421 caccccattg atgacctgtt atttaacaga ttcgctggat cctaatgagc tggagcgcgg
    87481 atttaatgaa gccgtgttca ccgctgcaaa actttacccg gcaaacgcaa ccactaactc
    87541 cagccacggc gtgacgtcaa ttgacgcaat catgccggta cttgagcgca tggaaaaaat
    87601 cggtatgccg ctactggtgc atggtgaagt gacacatgca gatatcgaca tttttgatcg
    87661 tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa
    87721 agtcgttttt gagcacatca ccaccaaaga tgctgccgac tatgtccgtg acggaaatga
    87781 acggctggct gccaccatca ctccgcagca tctgatgttt aaccgcaacc atatgctggt
    87841 tggtggcgtg cgtccgcacc tgtattgttt acccatcctc aaacgcaata ttcaccaaca
    87901 ggcattgcgt gaactggtcg ccagcggttt taatcgagta ttcctcggta cggattctgc
    87961 gccacatgca cgtcatcgca aagagagcag ttgcggctgc gcgggctgct tcaacgcccc
    88021 aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg aatgctctgc agcactttga
    88081 agcattctgt tctgtaaacg gcccgcagtt ctatggcttg ccggtcaacg acacattcat
    88141 cgaactggta cgtgaagagc aacaggttgc tgaaagcatc gcactgactg atgacaccct
    88201 ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt aaacaataaa aatcgtagct
    88261 tcctgttgtc attaggttat tttacctgta taaataacca gtatattcaa cagggggcta
    88321 ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt gccagctggg gctattgacg
    88381 ccctggctgg cgaactttcc cgccgtattc agtatgcgtt tcctgataat gaaggccacg
    88441 tatcggtacg ttatgccgca gcgaataatt tatcggttat tggcgcaaca aaagaagata
    88501 aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag cgccgatgac tggtttgtca
    88561 gcgaataata tgcagtgatt tttgtttttg ccggatcgcc ccggcttttt tattttactt
    88621 acgaattttg agtacaaaat ttctacactt cctcaaaata attgcacaat atgctaaaaa
    88681 aatatgaatg acatgctgtt ttattgttca tataaatctt aaaagcagaa atttatctgt
    88741 tccccccttt aaaaattcgg tgaataccct tacttattgg tatactgaag ttgcttcaga
    88801 taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg ggccatgatg
    88861 gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat cagcaccgtt
    88921 gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa gtccgagcag
    88981 gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag acaatttatt
    89041 tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt aaacgagtat
    89101 cggcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt gcctttacca
    89161 tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga gagcagagag
    89221 aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc tgccgccggg
    89281 tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg acgcccatat gccaccgcat
    89341 caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg tgaaggcgaa
    89401 aagtatgtcc cgttggtcct ccgcgcgcaa acgctgtggg atgaactttc ccgccacaac
    89461 gaagatgatc ccatttttgt acgctctggt gtcattaacc tcggcccggc tgactccgca
    89521 tttctcgcca acgtcgccca cagcgccgaa cagtggcaac tcaacgttga aaagctcgat
    89581 gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta catcggctta
    89641 tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg gatccaactg
    89701 gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg tcaccgcaat tcgtcatgac
    89761 gatgatggcg taactattga aacggttgac ggtgagtatc aggcgaaaaa agcgattgtc
    89821 tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc cgtacgtaaa
    89881 gtatttgcct ggtatcaggc cgatggccgc tatagcgtga agaataaatt cccggcgttt
    89941 accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa cgacgcgttg
    90001 aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg tgttccgttt
    90061 gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct tgcgcaatgt attgccgggt
    90121 atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga cgaagatttt
    90181 attatcgata ccctacccgg ccacgataat acactgctca ttaccggcct gagtgggcac
    90241 ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca agaccaaaaa
    90301 agcgattttg atttgacgcc attcaggctt tcccgcttcc aataatcatc attacggcct
    90361 ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac tatctcatca
    90421 ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg gcaattatgg
    90481 atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac aaatatttaa
    90541 tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta cagcactcac
    90601 cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta ttaattcaaa
    90661 attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca attcattctc
    90721 cgacacgcca ttttctttct ctcgcaaatt cgtattggat gcacttatgt cattcacaaa
    90781 tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg caattattgt
    90841 ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg atggctggta
    90901 tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg ggctggttat
    90961 tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt attcgcccat
    91021 gactcgtctt gccgcaaaag ctggacatct cgcattgtgc ctattgctgt tcgccatcgg
    91081 catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt ttggctggtt
    91141 tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg gtgccctgca
    91201 tttttggctc gcgtggagcg tcgtagtact gtctgttatg cacggattta tggccctgaa
    91261 gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt cgtcatctga
    91321 ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt ccctgatgtt
    91381 ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc agcacgcctt
    91441 tgttaacttc cgcatacagc atcttggcta tagctggtta tatggcacct ttaaagattt
    91501 cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga atgtgacaat
    91561 taacaccacc agcgtcgata ctaaccacgc cgaacgcgat aaacatcttc gcagtgcgga
    91621 ttttctcaat accacaaaat atccacaggc aacattcacc tccaccagcg tgaagaaaga
    91681 cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca aacccgtcac
    91741 gctggaagcg aaattaattg gtcagggcga cgacccatgg ggcggtaaac gtgcaggctt
    91801 tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt taggtccagc
    91861 ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt aagagtgaaa
    91921 tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag gaatacccag
    91981 tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt cacgtcctgc
    92041 ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa tctcactaca
    92101 acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt ggcagccatc
    92161 atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat gcgcgataat
    92221 ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc caataaaacg
    92281 caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc cctcgatatg
    92341 ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac aacgaatgcc
    92401 gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct cgactgcttt
    92461 tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa agtgccccac
    92521 ttcatactca tagtggttac gcatgtcgat aaatagtgca tcgggatcgt caagcatggc
    92581 gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt gaggatcgtc
    92641 aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga aggatttccc
    92701 gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga aggcatagag
    92761 ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt taatgccctc
    92821 atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat ataaagcgtc
    92881 acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg
    92941 ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac
    93001 tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa
    93061 ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa attatttgtt
    93121 atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa
    93181 aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat atgacgaatc
    93241 tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg
    93301 gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc ggttgtggat
    93361 taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg
    93421 aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg
    93481 aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg gaccgccgaa
    93541 tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac
    93601 gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg cggcagtttg
    93661 gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc
    93721 tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag
    93781 gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccagat catgattacg
    93841 gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg accacgaccg
    93901 gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta
    93961 agccagatgg caaagggtat caattgatta tgctgccacc agagtgttct ccgccactgg
    94021 atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg
    94081 caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt
    94141 cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt accacagcaa
    94201 atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata
    94261 attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg
    94321 ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg atcgtcgcct
    94381 ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc ttacccctct
    94441 acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc ggtattgtct
    94501 tcagtataat gggttcctaa acacatcatt ttaggtattg aatatgagac gggcaatcag
    94561 ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc gacaatctaa
    94621 gctggttact gactggcttg ataagaaccc tgagttttac ttggattcat ccttgagctt
    94681 taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc taggtgactt
    94741 tttaaccgct atcgagaaag ggcttgttaa agctggtgat acgctactaa tcgagagttt
    94801 agaccgtctg tcacgtcagg atattgatat tgcttctgag ctattacgcc gtatcttaag
    94861 ggctggtgta gatgtagtca cactgagcga tggagaacat tacacacgag aaagtttaaa
    94921 agacccgtta gccctgatta aaagcattct gataatgcag cgggcgcatg aggaaagttt
    94981 gagaaagtct gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca tctccgaagg
    95041 tatcaaggta tctaggcgtt gtcctgcatg gttaaggctg aatgatgacc gtcgaacatt
    95101 cacaatcatt cctgataaag tggaagttgt taaacgagca tttgacctga gattacaggg
    95161 tttaagcttt tgggctatca ccagaacttt aaacgatgaa ggtcacttgt cattgaatca
    95221 atacacaccc aaacaaaagg ggtggtctga tactgctgta aagaaactgc tcagaaatcg
    95281 tgctgtgatt ggatgtttca cacct
//
LOCUS       DAJVQR010000002        93171 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVQR010000002 DAJVQR010000000
VERSION     DAJVQR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 93171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            4..79
                     /locus_tag="OQM52_000107"
     tRNA            4..79
                     /locus_tag="OQM52_000107"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Lys,seq:ttt)
     gene            110..235
                     /locus_tag="OQM52_000108"
     ncRNA           110..235
                     /ncRNA_class="other"
                     /locus_tag="OQM52_000108"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            355..696
                     /locus_tag="OQM52_000109"
     CDS             355..696
                     /locus_tag="OQM52_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1562116.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(687..1613)
                     /locus_tag="OQM52_000110"
     CDS             complement(687..1613)
                     /locus_tag="OQM52_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1562117.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1703..2701
                     /locus_tag="OQM52_000111"
     CDS             1703..2701
                     /locus_tag="OQM52_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1562118.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2698..2916)
                     /locus_tag="OQM52_000112"
     CDS             complement(2698..2916)
                     /locus_tag="OQM52_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1562119.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2918..4933)
                     /gene="ligA"
                     /locus_tag="OQM52_000113"
     CDS             complement(2918..4933)
                     /gene="ligA"
                     /locus_tag="OQM52_000113"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1562120.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(5004..5990)
                     /gene="zipA"
                     /locus_tag="OQM52_000114"
     CDS             complement(5004..5990)
                     /gene="zipA"
                     /locus_tag="OQM52_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1562121.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6220..6981
                     /gene="cysZ"
                     /locus_tag="OQM52_000115"
     CDS             6220..6981
                     /gene="cysZ"
                     /locus_tag="OQM52_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1562122.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7166..8137
                     /gene="cysK"
                     /locus_tag="OQM52_000116"
     CDS             7166..8137
                     /gene="cysK"
                     /locus_tag="OQM52_000116"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1562123.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8521..8778
                     /gene="ptsH"
                     /locus_tag="OQM52_000117"
     CDS             8521..8778
                     /gene="ptsH"
                     /locus_tag="OQM52_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1562124.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8823..10550
                     /gene="ptsI"
                     /locus_tag="OQM52_000118"
     CDS             8823..10550
                     /gene="ptsI"
                     /locus_tag="OQM52_000118"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1562125.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10591..11100
                     /gene="crr"
                     /locus_tag="OQM52_000119"
     CDS             10591..11100
                     /gene="crr"
                     /locus_tag="OQM52_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1562126.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11142..11993)
                     /gene="pdxK"
                     /locus_tag="OQM52_000120"
                     /gene_synonym="thiJ"
     CDS             complement(11142..11993)
                     /gene="pdxK"
                     /locus_tag="OQM52_000120"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1562127.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12098..12472
                     /locus_tag="OQM52_000121"
     CDS             12098..12472
                     /locus_tag="OQM52_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1562128.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12505..13228
                     /locus_tag="OQM52_000122"
                     /pseudo
     CDS             12505..13228
                     /locus_tag="OQM52_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13432..14343)
                     /gene="cysM"
                     /locus_tag="OQM52_000123"
     CDS             complement(13432..14343)
                     /gene="cysM"
                     /locus_tag="OQM52_000123"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1562129.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14477..15574)
                     /gene="cysA"
                     /locus_tag="OQM52_000124"
     CDS             complement(14477..15574)
                     /gene="cysA"
                     /locus_tag="OQM52_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1562130.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15564..16439)
                     /gene="cysW"
                     /locus_tag="OQM52_000125"
     CDS             complement(15564..16439)
                     /gene="cysW"
                     /locus_tag="OQM52_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1562131.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16439..17272)
                     /gene="cysT"
                     /locus_tag="OQM52_000126"
     CDS             complement(16439..17272)
                     /gene="cysT"
                     /locus_tag="OQM52_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1562132.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17272..18288)
                     /gene="cysP"
                     /locus_tag="OQM52_000127"
     CDS             complement(17272..18288)
                     /gene="cysP"
                     /locus_tag="OQM52_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1562133.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18592..19383)
                     /gene="ucpA"
                     /locus_tag="OQM52_000128"
     CDS             complement(18592..19383)
                     /gene="ucpA"
                     /locus_tag="OQM52_000128"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1562134.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19570..20370)
                     /gene="murR"
                     /locus_tag="OQM52_000129"
     CDS             complement(19570..20370)
                     /gene="murR"
                     /locus_tag="OQM52_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1562135.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20534..21430
                     /gene="murQ"
                     /locus_tag="OQM52_000130"
     CDS             20534..21430
                     /gene="murQ"
                     /locus_tag="OQM52_000130"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1562136.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21434..22858
                     /gene="murP"
                     /locus_tag="OQM52_000131"
     CDS             21434..22858
                     /gene="murP"
                     /locus_tag="OQM52_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCS1562137.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(23038..23937)
                     /gene="yfeX"
                     /locus_tag="OQM52_000132"
     CDS             complement(23038..23937)
                     /gene="yfeX"
                     /locus_tag="OQM52_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1562138.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24033..24608)
                     /locus_tag="OQM52_000133"
     CDS             complement(24033..24608)
                     /locus_tag="OQM52_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1562139.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24669..25118)
                     /locus_tag="OQM52_000134"
     CDS             complement(24669..25118)
                     /locus_tag="OQM52_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1562140.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25105..25530)
                     /locus_tag="OQM52_000135"
     CDS             complement(25105..25530)
                     /locus_tag="OQM52_000135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1562141.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25744..26613
                     /gene="amiA"
                     /locus_tag="OQM52_000136"
     CDS             25744..26613
                     /gene="amiA"
                     /locus_tag="OQM52_000136"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1562142.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26617..27516
                     /gene="hemF"
                     /locus_tag="OQM52_000137"
     CDS             26617..27516
                     /gene="hemF"
                     /locus_tag="OQM52_000137"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1562143.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27522..28574)
                     /gene="eutR"
                     /locus_tag="OQM52_000138"
                     /pseudo
     CDS             complement(27522..28574)
                     /gene="eutR"
                     /locus_tag="OQM52_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28620..29120)
                     /gene="eutK"
                     /locus_tag="OQM52_000139"
     CDS             complement(28620..29120)
                     /gene="eutK"
                     /locus_tag="OQM52_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1562144.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29133..29792)
                     /gene="eutL"
                     /locus_tag="OQM52_000140"
     CDS             complement(29133..29792)
                     /gene="eutL"
                     /locus_tag="OQM52_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1562145.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30974..31765
                     /locus_tag="OQM52_000141"
     CDS             30974..31765
                     /locus_tag="OQM52_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562146.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32485..32880
                     /locus_tag="OQM52_000142"
     CDS             32485..32880
                     /locus_tag="OQM52_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1562147.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32898..33161
                     /locus_tag="OQM52_000143"
     CDS             32898..33161
                     /locus_tag="OQM52_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562148.1"
                     /translation="MHSLDSYFQRTTAPKSAAQEHREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            33184..33726
                     /locus_tag="OQM52_000144"
     CDS             33184..33726
                     /locus_tag="OQM52_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562149.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33865..34152
                     /locus_tag="OQM52_000145"
     CDS             33865..34152
                     /locus_tag="OQM52_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562150.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            34191..34397
                     /locus_tag="OQM52_000146"
     CDS             34191..34397
                     /locus_tag="OQM52_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562151.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34709..35194
                     /locus_tag="OQM52_000147"
     CDS             34709..35194
                     /locus_tag="OQM52_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1562152.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            35175..35453
                     /locus_tag="OQM52_000148"
     CDS             35175..35453
                     /locus_tag="OQM52_000148"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562153.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35446..36906
                     /locus_tag="OQM52_000149"
     CDS             35446..36906
                     /locus_tag="OQM52_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1562154.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36940..37749
                     /gene="murI"
                     /locus_tag="OQM52_000150"
     CDS             36940..37749
                     /gene="murI"
                     /locus_tag="OQM52_000150"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1562155.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37868..38562)
                     /locus_tag="OQM52_000151"
     CDS             complement(join(37868..38316,38316..38562))
                     /locus_tag="OQM52_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1562156.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(39081..40454)
                     /gene="eutA"
                     /locus_tag="OQM52_000152"
     CDS             complement(39081..40454)
                     /gene="eutA"
                     /locus_tag="OQM52_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS1562157.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40429..40908)
                     /gene="eutP"
                     /locus_tag="OQM52_000153"
     CDS             complement(40429..40908)
                     /gene="eutP"
                     /locus_tag="OQM52_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1562158.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40921..41256)
                     /gene="eutS"
                     /locus_tag="OQM52_000154"
     CDS             complement(40921..41256)
                     /gene="eutS"
                     /locus_tag="OQM52_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS1562159.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(41549..43828)
                     /gene="maeB"
                     /locus_tag="OQM52_000155"
     CDS             complement(41549..43828)
                     /gene="maeB"
                     /locus_tag="OQM52_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1562160.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            44117..45067
                     /gene="tal"
                     /locus_tag="OQM52_000156"
     CDS             44117..45067
                     /gene="tal"
                     /locus_tag="OQM52_000156"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1562161.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            45087..47090
                     /gene="tkt"
                     /locus_tag="OQM52_000157"
     CDS             45087..47090
                     /gene="tkt"
                     /locus_tag="OQM52_000157"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1562162.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(47193..48236)
                     /locus_tag="OQM52_000158"
     CDS             complement(47193..48236)
                     /locus_tag="OQM52_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1562163.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(48362..48937)
                     /gene="nudK"
                     /locus_tag="OQM52_000159"
     CDS             complement(48362..48937)
                     /gene="nudK"
                     /locus_tag="OQM52_000159"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1562164.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(49005..50984)
                     /gene="aegA"
                     /locus_tag="OQM52_000160"
     CDS             complement(49005..50984)
                     /gene="aegA"
                     /locus_tag="OQM52_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1562165.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            51190..52907
                     /gene="narQ"
                     /locus_tag="OQM52_000161"
                     /pseudo
     CDS             51190..52907
                     /gene="narQ"
                     /locus_tag="OQM52_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            53109..56222
                     /gene="acrD"
                     /locus_tag="OQM52_000162"
     CDS             53109..56222
                     /gene="acrD"
                     /locus_tag="OQM52_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS1562166.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(56321..56380)
                     /gene="ypfM"
                     /locus_tag="OQM52_000163"
     CDS             complement(56321..56380)
                     /gene="ypfM"
                     /locus_tag="OQM52_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1562167.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            56761..57117
                     /locus_tag="OQM52_000164"
     CDS             56761..57117
                     /locus_tag="OQM52_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1562168.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            57121..58248
                     /gene="dapE"
                     /locus_tag="OQM52_000165"
     CDS             57121..58248
                     /gene="dapE"
                     /locus_tag="OQM52_000165"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1562169.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            58276..58476
                     /locus_tag="OQM52_000166"
     CDS             58276..58476
                     /locus_tag="OQM52_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1562170.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(58586..59284)
                     /gene="ypfH"
                     /locus_tag="OQM52_000167"
     CDS             complement(58586..59284)
                     /gene="ypfH"
                     /locus_tag="OQM52_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1562171.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFALLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(59358..61373)
                     /gene="tmcA"
                     /locus_tag="OQM52_000168"
     CDS             complement(59358..61373)
                     /gene="tmcA"
                     /locus_tag="OQM52_000168"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1562172.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(61388..62251)
                     /locus_tag="OQM52_000169"
     CDS             complement(61388..62251)
                     /locus_tag="OQM52_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1562173.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(62419..63132)
                     /gene="purC"
                     /locus_tag="OQM52_000170"
     CDS             complement(62419..63132)
                     /gene="purC"
                     /locus_tag="OQM52_000170"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1562174.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIDLNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(63345..64379)
                     /gene="bamC"
                     /locus_tag="OQM52_000171"
     CDS             complement(63345..64379)
                     /gene="bamC"
                     /locus_tag="OQM52_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1562175.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(64396..65274)
                     /gene="dapA"
                     /locus_tag="OQM52_000172"
     CDS             complement(64396..65274)
                     /gene="dapA"
                     /locus_tag="OQM52_000172"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1562176.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            65420..65992
                     /locus_tag="OQM52_000173"
     CDS             65420..65992
                     /locus_tag="OQM52_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1562177.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            65992..66462
                     /gene="bcp"
                     /locus_tag="OQM52_000174"
     CDS             65992..66462
                     /gene="bcp"
                     /locus_tag="OQM52_000174"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1562178.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            66715..67332
                     /gene="hyfA"
                     /locus_tag="OQM52_000175"
     CDS             66715..67332
                     /gene="hyfA"
                     /locus_tag="OQM52_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1562179.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            67332..69349
                     /gene="hyfB"
                     /locus_tag="OQM52_000176"
                     /pseudo
     CDS             67332..69349
                     /gene="hyfB"
                     /locus_tag="OQM52_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            69360..70307
                     /gene="hyfC"
                     /locus_tag="OQM52_000177"
     CDS             69360..70307
                     /gene="hyfC"
                     /locus_tag="OQM52_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1562180.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            70324..71763
                     /gene="hyfD"
                     /locus_tag="OQM52_000178"
     CDS             70324..71763
                     /gene="hyfD"
                     /locus_tag="OQM52_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS1562181.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            71775..72425
                     /gene="hyfE"
                     /locus_tag="OQM52_000179"
     CDS             71775..72425
                     /gene="hyfE"
                     /locus_tag="OQM52_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1562182.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            72430..74022
                     /gene="hyfF"
                     /locus_tag="OQM52_000180"
     CDS             72430..74022
                     /gene="hyfF"
                     /locus_tag="OQM52_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1562183.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            74012..75726
                     /gene="hyfG"
                     /locus_tag="OQM52_000181"
                     /pseudo
     CDS             74012..75726
                     /gene="hyfG"
                     /locus_tag="OQM52_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            75736..76281
                     /gene="hyfH"
                     /locus_tag="OQM52_000182"
     CDS             75736..76281
                     /gene="hyfH"
                     /locus_tag="OQM52_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1562184.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            76278..77036
                     /gene="hyfI"
                     /locus_tag="OQM52_000183"
     CDS             76278..77036
                     /gene="hyfI"
                     /locus_tag="OQM52_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1562185.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            77029..77442
                     /gene="hyfJ"
                     /locus_tag="OQM52_000184"
     CDS             77029..77442
                     /gene="hyfJ"
                     /locus_tag="OQM52_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1562186.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            77472..79301
                     /gene="hyfR"
                     /locus_tag="OQM52_000185"
     CDS             77472..79301
                     /gene="hyfR"
                     /locus_tag="OQM52_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCS1562187.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            79302..79484
                     /locus_tag="OQM52_000186"
     CDS             79302..79484
                     /locus_tag="OQM52_000186"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCS1562188.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            79506..80377
                     /locus_tag="OQM52_000187"
                     /pseudo
     CDS             79506..80377
                     /locus_tag="OQM52_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(80471..81532)
                     /locus_tag="OQM52_000188"
     CDS             complement(80471..81532)
                     /locus_tag="OQM52_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1562189.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            81745..83208
                     /gene="bepA"
                     /locus_tag="OQM52_000189"
     CDS             81745..83208
                     /gene="bepA"
                     /locus_tag="OQM52_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1562190.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            83229..83588
                     /gene="arsC"
                     /locus_tag="OQM52_000190"
     CDS             83229..83588
                     /gene="arsC"
                     /locus_tag="OQM52_000190"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1562191.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(83721..84467)
                     /gene="hda"
                     /locus_tag="OQM52_000191"
     CDS             complement(83721..84467)
                     /gene="hda"
                     /locus_tag="OQM52_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1562192.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(84517..85806)
                     /gene="uraA"
                     /locus_tag="OQM52_000192"
     CDS             complement(84517..85806)
                     /gene="uraA"
                     /locus_tag="OQM52_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1562193.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(85892..86518)
                     /gene="upp"
                     /locus_tag="OQM52_000193"
     CDS             complement(85892..86518)
                     /gene="upp"
                     /locus_tag="OQM52_000193"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1562194.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            86842..87879
                     /gene="purM"
                     /locus_tag="OQM52_000194"
     CDS             86842..87879
                     /gene="purM"
                     /locus_tag="OQM52_000194"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1562195.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            87879..88517
                     /gene="purN"
                     /locus_tag="OQM52_000195"
     CDS             87879..88517
                     /gene="purN"
                     /locus_tag="OQM52_000195"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1562196.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            88689..90755
                     /gene="ppk1"
                     /locus_tag="OQM52_000196"
     CDS             88689..90755
                     /gene="ppk1"
                     /locus_tag="OQM52_000196"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1562197.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            90760..92301
                     /gene="ppx"
                     /locus_tag="OQM52_000197"
     CDS             90760..92301
                     /gene="ppx"
                     /locus_tag="OQM52_000197"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1562198.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(92340..>93171)
                     /gene="pdeF"
                     /locus_tag="OQM52_000198"
     CDS             complement(92340..>93171)
                     /gene="pdeF"
                     /locus_tag="OQM52_000198"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1562199.1"
                     /translation="TNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQP
                     ITGMRGDVYHEILLRMKGENDELIGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENR
                     AKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQI
                     TLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASI
                     CHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA
                     "
BASE COUNT        22542 a        24325 c        23485 g        22819 t
ORIGIN      
        1 ttcgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg
       61 aatcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg
      121 atactcaaag attcgaacct gcagcaggtt tgagttgagc gcagcgaaac aacggagccg
      181 ctcgcggcga cggcccgaag ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgca
      241 aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgattt gtaagattga tttttcttct
      301 caagttcctt gttcttctac cgatattacc tcgtacagtg acgaggactg aacaatggcc
      361 actatctctt caacttcaat tccttccata caaacacaga gtagtaacag ggcatcacaa
      421 ggtagcgatg ttgcttcaca aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa
      481 caaattaaag agattgtaga cactagcggc agtgctgaag acaagcaaaa acaggccgaa
      541 ttgattcaac agcaaatcac cctactggaa acacaactgg cgcagttgca gaaacagcag
      601 gcagaaaagg cgcaggagaa agagcagcgt ttgtctctga atgtatccct tctaaatcct
      661 gttgaaaaaa cgactcatat cgatatctat atctgataca acggatcccc ttcccgacca
      721 gccattagcg cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa
      781 agtgaacggt tcttcctgcg caccagcatc aactggcgtt caaccaccgg cgtaatgcgc
      841 ttaaccacca gtgggctacc ttcaggcaat ggtagcgcca gtgcaggaag aatactgatg
      901 ccgatgcctg cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata
      961 ttcgcctgaa taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg
     1021 taatcctgca acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccacg
     1081 gccaaagcgc tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat
     1141 tgcagatcgc ccactgggcc tggatcgatg acgatgccaa agtcgacatc tccctggcga
     1201 atactttcca tcacccactg ctgcggacga tcatgcagga caaactgaat atctggatag
     1261 cggcgatggc tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca
     1321 gcgacccgaa cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg
     1381 ttaagttcat ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc
     1441 accacttcac gcgtggttct gtccagcagg cgaacaccag tatgattttc cagctccttc
     1501 acactgtgac ttactgccga ctggctcaag ccgatacgct ctcctgcacg actaaaactt
     1561 ttctcctgcg ctactgtgac gaaaaccttt aattgtttta aagaataatt catctgtttt
     1621 cttcatgaat ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa
     1681 ccatcgatca tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac
     1741 cctgatcacg gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt
     1801 ctttgaaaat ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt
     1861 gtcgcgtgag gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg
     1921 cagcaccttc gtgctgtttc cgattctggg tgtactgttt gcctggtgga aaccggtaaa
     1981 tgtcgacccg atgctctact ccggttttct ctacttgtgc attctcccgg ctaccgtgca
     2041 gtctgcaatc gccttcacgt caatggcggg cggtaacgtc gcggcggcgg tttgttctgc
     2101 gtcagcatcc agcctgctgg ggattttcct ttcaccattg ctggttggtc tggtgatgaa
     2161 tgttcacggt gcaaggggca gccttgagca ggtcggtaaa attatgctgc aactgctgct
     2221 gccgtttgtg ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa
     2281 aaaatggatt gcgaaaactg accagacgtc cattctgttg gtggtttata cagcgttcag
     2341 cgaagccgtc gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt
     2401 ggtggtcagc tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg
     2461 actgagcttc aataaggcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct
     2521 ggcaaatggc atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt
     2581 gctgcccctg atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg
     2641 atacaaacgc cagaccgaac agttacaggc gcagcaggaa agcagcgccg ataaagctta
     2701 aagcggacgc ttcaggggct ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt
     2761 gatttgcatt agctcaccga gcttccctgc cgggaactca tctttgcggg caaaccacag
     2821 cagatactcc tccggcaggt caattaagcg acgccctttg tatttgccaa acggcattat
     2881 cgtattggct atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca
     2941 tttccgcttc gtcgatgact tcaatgccca gttcctgcgc cttcgccagt ttagatcctg
     3001 cagcttcacc cgctatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc
     3061 ccagttcgac cagtcgagct ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa
     3121 gcaccacggt tttaccagca aacgggctgt caatctcttc cgcgttgata acgatcggcg
     3181 caggccagtg aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg
     3241 caaagaagtt gtgaacgtgg gatgcaacga caatgccaac atcaggcacc ttttgcagct
     3301 cttcaatcga agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc agacctgctg
     3361 cggtggcctc gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt
     3421 ctttcgcttt ttccagcgcg ttaaccacgt tttgtgccaa ttttggcccc atacgctcca
     3481 gtccggtcag tttgcctgcg gtgagtttga acagatctgc cggagtatgg acatattctt
     3541 tttcaaccag ctgatcgatg attttgtcgc ccattccgtc aacatccatc gcacggcggg
     3601 aaacaaagtg tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc
     3661 gggcaaccgc ttcaccttcc acacgctcaa cgtcagaacc acataccgga caatgcgtcg
     3721 ggaatacaac ctcacgggta tcttccgggc gttcagaaag cacgacgtta accacctgcg
     3781 ggatcacgtc gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt
     3841 cgatttcatc cgcattgtgt aaggttgcgt tactcaccag cacgccagca acatggacag
     3901 gttccagacg cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac
     3961 gcacaaaggt catctgctcc tgcgccggga atttaaacgc aaccgcccag cgcggggcac
     4021 gcgcgacaaa gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc accacgccgt
     4081 cgatatcaaa gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt
     4141 cttccgccga ttcacaaagc gttacccgat cgctgaccgg caccccccac tttttaaatt
     4201 gcagtaaacg gccaagatga gtatccggca gctcgccacc ttccagaaca ccaacgccat
     4261 agcagaaaaa agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac
     4321 cagctgccgc attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt
     4381 taatcttttc gaaccccgcc tgcggcagga acacttcacc acgcacttcc agacgcgccg
     4441 ggatattctc tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga
     4501 tatcttcccc ggtggtgcca tcgccacggg tcgcggcact gactaaaacg ccattttcat
     4561 acagaatact gacggcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt
     4621 tgtttttcag acggtcctgc acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa
     4681 cgttatccag tgacagcatt ggtacttcat ggcgtatctg gctgaaagct gccagcggcg
     4741 cagcacctac acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca
     4801 gctcgcgcag ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca
     4861 tcacatgata aagatattca tgatggcaaa gcgtcgttcg cagttctgtc agttgttgtt
     4921 cgattgattc catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga
     4981 ggagttaatt tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc
     5041 tggtactcgc gcaatttctg cggagtcatc atacggcgct gatcgtcaag cacgacaccg
     5101 cccacttcat cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg
     5161 tcaccgtaag acggtacctg cataaagata gtgacacccg gagtagtgaa atccttcatt
     5221 tcaggatcaa aggttcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg
     5281 ccatccgggc taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt
     5341 tgaatgctgt taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc
     5401 atgataatca ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca
     5461 ggctcaggct gtggtgccgc tacgggttct gcaggctgga aagcctgttg tgccggttgc
     5521 ggtgctgaat gcacaggctg cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac
     5581 ggctgttcag gctgcggctg ataggcaggc tgctgcaccg gctgcgctgg acgcggagca
     5641 tgttgcggcg gtacctgcgc ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac
     5701 gcataaggcg gtaggtactg gtgttgcggc gacggacgag cagcctcatg ctcctgagcg
     5761 ttagccgggg catgattcac gcggtgaaca cgaacctcac caacgccctc atcatcttcg
     5821 acatcctcgt cataagaatc gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg
     5881 cggaacatag aagatcgttc tttacggctg gtccagaaac catgtaccag taaagcgatt
     5941 atggcgatcg cgccaacaat gattaatatc agacgcaaat cctgcatcat tatattctct
     6001 gttgttctaa caccttgcca ccacggcaaa catttactca ctaagagtat ttgccgatta
     6061 cctcaagtgc aagtgcacta ttaactttca cagcacaaag atagatgaaa tcgtgctttt
     6121 tgctgttttt tcgaacatat cctaactgtc cattgcgcaa ttacccggta aaatacgcag
     6181 aattttcctg ggattggtca aaaggagctc atcctgatta tggtttcatc attcacatct
     6241 gccccacgca gcggttttta ctattttgcg caaggctgga agctcgtctc gcaacctggg
     6301 attcggcgtt tcgttatttt accgctgctg gtcaatattt tgttgatggg gggcgcattc
     6361 tggtggctct ttacacagct cgatgtctgg atcccgactt tcatgagtta cgttccggac
     6421 tggctacaat ggctgagtta tctgttgtgg cctctggctg tcatctctgt gctgttagtg
     6481 tttggctatt tcttctccac gattgctaac tggattgccg ctccgtttaa cggtttattg
     6541 gctgaacaac tggaagcacg attgactggc gctacaccgc cagataccgg gattttcggt
     6601 atcatgaaag atgtgccgcg aatcatgaaa cgcgaatggc aaaaatttgt ctggtatctg
     6661 ccgcgcgcaa ttgtattact aattctttac ttcatccctg gtattgggca aaccgtcgcg
     6721 ccggtactgt ggttcctgtt tagcgcctgg atgttagcca tccagtattg cgattacccc
     6781 ttcgataacc acaaagtgcc gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc
     6841 accaatatgc agtttggtgc tttaaccagc ctgtttacga tgatcccgct gcttaatctg
     6901 ttcatcatgc ccgttgccgt ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat
     6961 aaacacgcga tgtggcggta acaatctacc ggttattttg taaaccgttt gtgtgaaaca
     7021 ggggtggctt atgccgcccc ttattccatc ttgcatgtca ttatttccct tctgtatata
     7081 gatatgctaa atccttactt ccgcatattc tctgagcggg tatgctacct gttgtatccc
     7141 aatttcatac agttaaggac aggccatgag taagattttt gaagataact cgctgactat
     7201 cggtcacacg ccgctggttc gcctgaatcg catcggtaac ggacgcattc tggcgaaggt
     7261 ggaatctcgt aaccccagct tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga
     7321 tgccgaaaag cgcggcgtgc tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa
     7381 taccgggatt gcactggcct atgtagctgc cgctcgcggt tacaaactca ccctgaccat
     7441 gccagaaacc atgagtattg aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt
     7501 gctgacggaa ggtgctaaag gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc
     7561 cagcaatcca gagaaatacc tgctgctgca acaattcagc aatccggcaa accctgaaat
     7621 tcacgaaaaa accaccggtc cggagatctg ggaagatacc gacggtcagg ttgatgtatt
     7681 tattgctggc gttgggactg gcggtacgct gactggcgtc agccgctaca ttaaaggcac
     7741 caaaggcaag accgatctta tctctgtcgc cgttgagcca accgattctc cagttatcgc
     7801 ccaggtgctg gcaggtgaag agattaaacc tggcccgcat aaaattcagg gtattggcgc
     7861 tggttttatc ccggctaacc tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa
     7921 tgaagaagcc atttctaccg cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat
     7981 ctcttctgga gcagctgttg ccgcagcgtt gaaactacaa gaagatgaaa gctttaccaa
     8041 caagaatatt gtggttattc taccatcatc gggtgagcgt tatttaagca ccgcattgtt
     8101 tgccgatctc ttcactgaga aagaattgca acagtaatgc cagcttgtta aaaatgcgta
     8161 aaaaagcacc tttttaggtg cttttttgtg gcctgcttca aactttcgcc cctcctggca
     8221 ttgattcagc ctgtcggaac tggtatttaa ccagactaat tattttgatg cgcgaaatta
     8281 atcgttacag gaaaagccaa agctgaatcg attttatgat ttggttcaat tcttccttta
     8341 gcggcataat gtttaatgac gtacgaaacg tcagcggtca acacccgcca gcaatggact
     8401 gtattgcgct cttcgtgcgt cgcgtctgtt aaaaactggc gctaacaata caggctaaag
     8461 tcgaaccgcc aggctagact ttagttccac aacactaaac ctataagttg gggaaataca
     8521 atgttccagc aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc
     8581 cagtttgtaa aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa
     8641 agcgccagcg cgaaaagcct gtttaaactg cagactctgg gcctgactca aggtaccgtt
     8701 gtgactatct ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg
     8761 atggcggaac tcgagtaatt tcccgggttc ttttaaaaat cagtcacaag taaggtaggg
     8821 ttatgatttc aggcatttta gcatccccgg gtatcgcttt cggtaaagct ctgcttctga
     8881 aagaagacga aattgtcatt gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag
     8941 ttgaacgttt tctgagcggt cgtgccaagg catcagccca gctggaaacg atcaaaacga
     9001 aagctggtga aacgttcggt gaagaaaaag aagccatctt tgaagggcat attatgctgc
     9061 tcgaagatga ggagctggag caggaaatca tagccctgat taaagataaa cacatgacag
     9121 ctgacgcagc tgctcatgaa gttatcgaag gtcaggcttc tgccctggaa gagctggatg
     9181 atgaatacct gaaagaacgt gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca
     9241 acatcctggg cctgaagatt atcgacctga gcgccattca ggatgaagtc attctggttg
     9301 ccgctgacct gacgccgtcc gaaaccgcac agctgaacct gaagaaggtg ctgggtttca
     9361 tcaccgacgc gggtggccgt acttcccaca cctctatcat ggcgcgttct ctggaactgc
     9421 ctgctatcgt gggtaccggt agcgtcacct ctcaggtgaa aaatgacgac tatctgattc
     9481 tggatgccgt aaataatcag gtttacgtca atccaaccaa cgaagttatt gataaaatgc
     9541 gcgctgttca ggagcaagtg gcttctgaaa aagcagagct tgctaaactg aaagatctgc
     9601 cagctattac gctggacggt caccaggtag aagtatgcgc taacattggt acggttcgtg
     9661 acgttgaagg tgcagagcgt aacggcgctg aaggcgttgg tctgtatcgt actgagttcc
     9721 tgttcatgga ccgcgacgcg ctgcccactg aagaagaaca gtttgctgct tacaaagcag
     9781 tggctgaagc gtgtggctcg caggcggtta tcgttcgtac catggacatc ggcggcgaca
     9841 aagagctgcc atacatgaac ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta
     9901 tccgtatcgc gatggatcgt aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg
     9961 cctcggcttt cggtaaattg cgcattatgt tcccgatgat catctctgtt gaagaagtgc
    10021 gtgcactgcg caaagagatc gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt
    10081 ttgacgagtc aattgaaatc ggcgtaatgg tggaaacacc ggctgccgca acaattgcac
    10141 gtcatttagc caaagaagtt gatttcttta gtatcggcac caatgattta acgcagtaca
    10201 ctctggcagt tgaccgtggt aatgatatga tttcacacct ttaccagcca atgtcaccgt
    10261 ccgtgctgaa cttgatcaag caagttattg atgcttctca tgctgaaggc aaatggactg
    10321 gcatgtgtgg tgagcttgct ggcgatgaac gtgctacact tctgttgctg gggatgggtc
    10381 tggacgaatt ctctatgagc gccatttcta tcccgcgcat taagaagatt atccgtaaca
    10441 cgaacttcga agatgcgaag gtgttagcag agcaggctct tgctcaaccg acaacggacg
    10501 agttaatgac gctggttaac aagttcattg aagaaaaaac aatctgctaa tccacgagat
    10561 gcggcccaat ttactgctta ggagaagatc atgggtttgt tcgataaact gaaatctctg
    10621 gtttccgacg acaaaaagga taccggaact attgagatca ttgctccgct ctctggcgag
    10681 atcgtcaata tcgaagacgt gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt
    10741 attgctatca aaccaacggg taacaaaatg gtcgcgcctg tagacggtac cattggtaaa
    10801 atctttgaaa ccaatcacgc gttctctatc gaatctgata gcggcgttga actgttcgtc
    10861 cacttcggta tcgacaccgt tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa
    10921 ggtcagcgcg tgaaagttgg cgatactgtc attgaatttg atctgccgct gctggaagag
    10981 aaagccaagt ctaccctgac tccggttgtt atctccaaca tggacgaaat caaagaactg
    11041 atcaaactgt ccggtagcgt aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa
    11101 ttcttgccgc agtgaaaaat ggcgcccatc ggcgccattt tttatgcttc cgccagcggc
    11161 ggcaaaatca attcatcgct ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc
    11221 aaccccgcac ggtgcaccgc atcgtttaac gccttccctt tcagcaaacc actgataagc
    11281 tgagcacaaa acaggtcgcc agtccctttc aggtcggttt ttacccgtga atgggaaatg
    11341 acattcacgc tgtcggcact gaccaccacc acctgcatct cctgattttc ttcattaccg
    11401 gaggcgctgg taatcaccac ccattttaat gtgtctgaaa gcagactttt tgcggcagca
    11461 atggcactgt cgagatcgcg gcaattttta ccggtcagga tttccagctc aaagatattg
    11521 ggggtaattc cctgcgccag cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt
    11581 ttgacataaa ttccgctatc aatatcgcca atcaccggat cgaccatgat caataggtca
    11641 ggatggtctt tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt
    11701 cccatatagc ccgtggttac agcacgaagt tggcgcagcg catcacgctc ctgaagcgca
    11761 cgcaaatagc cgctaaacca ttcgtccgga atcgcaccac cgtagaaagt gtcataatgc
    11821 ggcgtattgc tcagcaatac cgtcggcacg gcaaagacat tcaagccgtt ctgttttata
    11881 gcaggcaccg caatgctgtt gcctacgctt ccgtacacta cctgcgactg cacggcgacg
    11941 atatccgcct gcagcgccct actcttatcg ttaaacaaca acaaactact catttaattt
    12001 tttctccttg ccgatgatcc tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt
    12061 caaatcggct attacaaaac aaagataagg taattcagtg aagaaaatca tttgtctggc
    12121 cattacgctg ctaatgacac tccccgctta cgcgaagtta actgttcatg aagaagcccg
    12181 catcaacgcc atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg
    12241 tgatgaacat acctgcgatg aagcggtttc ccacctgcgt ctgaagctcg gcaatacccg
    12301 taaccgcatt gacactgccg agcagtttat tgataaggta gcttcgtcgt catcgattac
    12361 cgggaagccg tatatcgtga agatccccgg taagagcgat gagaacgcac agcctttttt
    12421 acatgcgtta attgcgcaga cggataaaac ggtgcctgcg gaaggaaatt aatccgcttt
    12481 ggtaaggcat ttacaggagg taacatgaaa aaatgcttta tttatcacga tgaaaaatcg
    12541 aataaatttt ggtggataga ttacgaaggg gatagtttag ctgtcaacta tggcaaggta
    12601 ggtagtattg gtaaattcca tacaaaagaa ttcgataatg aagaacagtg tctgaaagaa
    12661 gccagtaaat tgattgccgc aaaaatgaaa aaaggatatc aagaaactcc agactttaac
    12721 ttcatggatc tctactattt tgatgatgaa gagattgggt tacatcttaa aacgtcacac
    12781 ccaaacttcc agtgccattt tactgatcca ctttatatgt gttgctggga tgaagaatct
    12841 ccttttggta gcgatgaagg tgctgatgct ctaaacgaac ttgaaaatag cctccgttct
    12901 ggactgtgct gatttccctc aaatgttaat tgaaactatg tggggtatga aatacatcgc
    12961 tatggacagt attcttgaag aggatgtgcg tgcgcaatta ctagccgatg aaatgagctc
    13021 tatccagagc aatatgatta cctacgcgac tgcattcggt cagatcaaag tcatgggtaa
    13081 aatctcccat aaacttaaaa agatgggact caatgcacta gcgcgtcatc agcttaccgc
    13141 aaaaattctt caatggggtg acggtcagga ctcaccaata cttcaaaaaa tgattgatga
    13201 ccttactgct tttccacacg aaaattaaaa actgcatttg tcggcagcaa caactgccga
    13261 caaatcagat tattgacaaa gtgcactttg tttatgccgg atgcggcgta aacgcctgat
    13321 ccggcataaa aatattgcta attcaatata ttgcagaaac attgtaggcc tgataagcgg
    13381 agcgcatcag gcagttttgc gtttgtcatc agtctccaat gctattaatc cttaaatccc
    13441 tgccccctga ctaaaatgct cttccccaaa cactccggtg gagaggtagc gatcgccacg
    13501 atcgcagatg atcgctacca ccaccgcacc agggttagct tttgccaccc gcagtgctcc
    13561 agcaaccgcg ccgcctgagc tgacgccaca gaatattcct tcccgcaccg ccagttcgcg
    13621 catggtgttt tccgcatcgc gctgatgaat atccagcacc tcatccacca gcgcagcgtt
    13681 gaaaatcccc ggcagatatt ccgcaggcca gcggcgaatg cccggaatac tgctgccttc
    13741 ttccggttgc aggccaacaa tggtcaccgg tttggattgt tcgcgcataa agcatgaaac
    13801 gccggtgata gtgccggtcg tccccatgct ggagacaaaa tgagtgatgc gcccgccggt
    13861 ttgctgccag atttccggcc cggtggtggt gtaatgcgca taagggttat cgggattatt
    13921 gaactgatcg agcagctttc cttcgccacg attcgccatc tccagcgcca gatcgcgcgc
    13981 accttccatg ccctgctctt tggtgacaag aatcagttcc gcaccataag cacgcatcgc
    14041 cgcacggcgt tcctggctca tgttgtcggg catcagcaat ttcatgcgat agcctttcag
    14101 cgcggcaatc attgccagcg caatgccggt gttaccactg gtggcttcga ttaagacatc
    14161 acccggttta atttccccgc gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc
    14221 tttcaccgaa cctgccgggt tattgccttc cagttttaac cacacttcac tgccgttatc
    14281 cggccccatt cgctgcaact tcaccagagg cgtattgcct attgtttgtt ctaatgtact
    14341 cacaatctct atccatacgt ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct
    14401 acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta
    14461 acattcactc aacctatcag gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa
    14521 tacgctcgtc gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccat
    14581 gctgcggggc atcgtcgcca tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg
    14641 gctgcaccac taattgggtg tagtgacctt tcgggctggc ttccagtacc tgtaccggca
    14701 gcggcgaatc gagactggta cggcggctga tatccacttc ccacgggcgc aggaagagat
    14761 ccaccggccc ctgatacgca ggtgtgtagc ccagcggcca gcgatgcgcg ccaacatgga
    14821 actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagtacaa
    14881 aacgggtcgc tggttcgcgc catacctgat tcggcgcatc agcctgttca atattcccct
    14941 gactcatcac cactacacga tcggctactt cggtcgcctc ttcctggtcg tgggtcacaa
    15001 acacgctggt gaatttcagc tcttcatgca gttgacgcag ccagcgacgc agctctttac
    15061 gcacctgcgc atccagcgcg ccgaacggtt catcaagcag cagaatttgc ggttctacag
    15121 ccagcgcgcg cgccagcgcc acacgctgtt tctggccgcc ggaaagctgc gccggataac
    15181 gatccgccag atgcgcaagc tggaccattt ccagcaattt tgtcactttc gctttgatgg
    15241 ctgcggcatt cgggcgctcg cgacgcggca gcaccgtcag gccaaaagtg atattgtcga
    15301 ataccgtcat atggcggaac agcgcgtaat gctggaacac gaaaccgact ttacgatcgc
    15361 gcgcgtgcag gcgactcacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct
    15421 ccagcccggc gataatccgc agcagcgtgg ttttcccgga accggacggc cccagcaacg
    15481 cgaccatctg accagaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa
    15541 aagacttctt gatattggca atctcaatgc tcatgatgct cctcctgctg cgcgcgtttt
    15601 tcctgattct ccaggcgcca ctgcaacata ctctttaaaa acagggtgat aatcgccatc
    15661 aacgtcaaca gcgcggcggc ggtaaaggag ccgacggtgt tgtagtcctg ctccaacaat
    15721 tcaatctgta acggcagcga aagtgtttcg ccgcgaatcg agccggaaac caccgacact
    15781 gcaccaaact cgccaatcgc gcgggcgttg gtcagcacca cgccgtaaag cagcgcccag
    15841 cgaatattcg gcaatgtgac gcgacggaac atctgccagc cggacgcgcc aagcaaaatc
    15901 gccgcttcgt cttcctggct gccctggctt aacatcaccg gcaccagctc gcgcaccaca
    15961 aacggacacg tcacaaagat ggtgaccagc accattccgg gccaggagaa cataatttgc
    16021 agattatgtt catccagcca gccgccgagc gggccgttgg agccgtagaa cagcagatac
    16081 accagaccgg caaccaccgg cgatacagca aacggaatgt ccagtagcgt cagcagtaac
    16141 tggcgtcccg ggaagttaaa gcgcgtcacc agccaggcca gcagaatgcc gaacaccagg
    16201 tttaccggta cggcaatcag cgcgatcatc accgtcagcc agatggcgtg cagcatgtcc
    16261 ggatcggcca gattctgtaa aaccggcatc agccccttgc tgaatgcctg cacgaagatg
    16321 taaatcatcg gcaccagcag gatgaacgcc gaaaccagca tcccgatgcc aatcagaaac
    16381 catttgcccc agttaatcgg gcgcgcgtca taacgcttca attgggtaac ttccgccatt
    16441 aatgacctac cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagca
    16501 gcagagatgc cgcgaggatc accgaagcaa tcgcgctcgc tgccgggtaa tcaaactcct
    16561 gtaagcgcac aaaaatcatc agcgacgtca cttccgtctt ccacgcgata tttccggcga
    16621 taaaaatcac cgcgccaaat tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca
    16681 ccagcgccgg agaaagctcc ggcagcacca ctttgcagaa actctgccag cgcgttgcac
    16741 caagcgtttc cgccgcttct tcatattccg ggcctaactc ttccagcacc ggctgcacgg
    16801 tacgcaccac aaacggaatg ctggtaaagg ccatcgccac cgcaatacca agccaggtat
    16861 aggtgacttt gatatcaaac ttcgccagcc attcaccgta aaaaccgttt acggaaaaga
    16921 gcgaggccag cgttaaaccg gcgactgccg ttggcagcgc aaagggtaaa tccatcagcg
    16981 catcaagcag cgtgcggccc ggaaagcgat aacgtgttaa aatccacgcc atcagcagac
    17041 cgaaaacgcc gttaaaaatc gatgccacaa acgccgacag cagcgtcact ttgtaggccg
    17101 cgaccacctg cgggttggtg atcacctccc agtactgcgc ccagctcatc tcagaaagct
    17161 gcatcaccag cgcagagagc ggcagcagca atatcaggca cacaaacagc agactggtgc
    17221 caaggcttaa agtaaagccc ggcagcacgc gtcttgaaga aactgcaaac attacttacg
    17281 ccccgccgct aacagcttgt ctaactcgcc gccgctggtg aagtgggttt tcatcacttc
    17341 cggccatgag ccaaatttgt cttccacgcg gaacagttcg gtctgcggga atttatcttt
    17401 cagtttgtcc atgacttccg ggttattcac acggtagtaa tagtcggtga tgatggtctg
    17461 cgcctgtggg ctgtagagcc agttcaggta ggcttttgcg gctttttccg taccgttggc
    17521 ctgcacattt ttatcgaccc acgccaccgg gaattccgcc agaatattgg ttttcgggat
    17581 caccacttca aagccctgtg cttcatactg tttacggatg ttgttcactt ccgattcaaa
    17641 gctaatcagc acatcgccca agccacgctc ggcaaaagtg gtggttgcgc cacgaccgcc
    17701 ggtatcgaac acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttggcttt
    17761 atcgccaccg tcagctttat ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt
    17821 acccgacgtt ttcgggttcg ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca
    17881 gtcgtggata ttcttcgggt tgcccttacg caccaggaag cccatggtgg agtagaacgg
    17941 cgagctatta ttcggcaggc gcgactgcca gtcagccggg atcagcttgc ctttgtcgtg
    18001 caggatttgt acgtcggtca cctggttata agtgacaacg tctgctttta agccctgcaa
    18061 aatcgccagc gcctgttttg atgacccggc atgagattgt tttatcgtca gtttgtcgcc
    18121 gccgttatct tttgcccatt gctgttcaaa cggcggattc agggcggcaa acagctcgcg
    18181 ggagacgtca taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagcagcag
    18241 agaagcgacc agcgcgagtg agttcttttt cagtaagtta acggccattg cgcaccctta
    18301 taaatttaat gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt
    18361 tttatatacc gtttggtgat ttggaagtta aaaagggaat aagaacttgg ttcaacaggc
    18421 gtaattgttg caggcagttt ggacacggac agcgcggaga aaccggagcg tacacatagt
    18481 acgtgaggat ttcgagcact gcccggggcc aaaatgacaa atgaaatagc ctggtgaact
    18541 tagttcaaga cgaaatcctc cccacacatg cagggaggaa acagaggtga atcagatacc
    18601 gacgctaacc gtctccggca gtgtgctgcc gccatcaatc acattctgtg taccggttaa
    18661 atagctggat tcatccgatg cgaggaaggc cgccagttcg ccgacttcca gcggatcggc
    18721 gaggcgacac agcgggattg cttttgccat ttcagtcagc accgattctg gatcttccgg
    18781 gttcgactgg cgggcaatgc tttccgccat tggcgtgcgg acgtatcccg ggcagatggc
    18841 gttaacgcga ataccggatt gcgcgtactc caccgccagc gatttagtca ggccaacaat
    18901 cgccgctttc gttaaggcat acgccgtttc gccaggatcg gccaccatat caccagtgac
    18961 tgaagacatc atcacaatgc gaccatcttt gcgcgcaatc atctccggca gcaccgcctt
    19021 cgtgacgttc catacgcctt taatattgat atcaatatgg aaatcgcgat cttcatcgct
    19081 catatcgagg aaactgccca gacgacaaac gcctgcgtta ttcaccagga tatcaatgcg
    19141 cccttctttt tccttcgcgc gtttgatagc tgcggctacc gacgccgggt cacgcacatc
    19201 ggcgacaacc gccgtacagc gatgaccacg accacacagt tcgtccgcta gcttttcgat
    19261 ctcaggggag atatccagca agattaggtt cgcgccatga cgtgcaaaag ttctggcaat
    19321 tccttcgcca attccctgca atgcgcccgt aatcagtgct gtcttgcccg tgagtttacc
    19381 cattttaatg ctctccttgt taatacagag cgttacactc cattccctct gaaaatacag
    19441 gataaacaaa atattgctgc ggatcagctc actaattcag gaaaatctca atccagtcgc
    19501 tgctttatgc atcatttcag gcgttgtgtt agctcactgc tgcgctgaat cattttcaat
    19561 gacgccacat cattgatgtt gcacaagacc aacaaacagt aaatcggtaa cagaattttg
    19621 cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcaccggaaa cggtatcgag
    19681 cgtgaaatgc gccagccgcc gcagcggaga gtcagccaga gaagtaatgg caatcaccgt
    19741 tgccccctgc tttcgtgccg cttctgcgca caagacaatc tcttttttac tgccgctgta
    19801 agagatagcg atttgtacat cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg
    19861 cgtgtcggct tcgcaggcca cgcgatagcc aattttcatc agtttgaacg acagatcgcg
    19921 ccccaccagc gccgatccgc ccaggcccgt tatctggata aacggtgctt tactgatgac
    19981 ctcaatgatt ttttgcagcc gcgcgtagtc gaacaacgca caggtttgct ccagcgccag
    20041 ctctttttcg cgattcagtt tgcgggcaat cacttccagc gaatcatcac tggtaatcga
    20101 actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt attcgccaat
    20161 taacgccata cgcaattcag taaatccttg cgcgccgagc ttctgggcaa atttcacaat
    20221 gctcgactgg ctgataccga gctgtttcgc catctgccgt gaagagacgg atttcagctc
    20281 gctcactctg gcccgcagaa agtcagcaat tttttgctcg ttcccggtaa attcactttc
    20341 cgcattgcgt attttcgtca ggtacaacat cttccactct cctgggctgg cagccaaaat
    20401 tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa tgattccttt
    20461 aagtctgtca attcgggata tatgattcca tacatctgca attatttgat cgtaaatagt
    20521 aaggtcacca ccgatgcaac tagaaaagat gattactgaa ggctcgaaca ccgcctcggc
    20581 tgaaattgac cgcgtatcga cgctggaaat gtgccggatt atcaacgatg aagataaaac
    20641 cgtaccgcct gccgttgatc gcgtcctgcc ggatatcgcc gcggcgatcg atgttatcca
    20701 cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct
    20761 ggggattctg gatgccagcg aatgtccgcc cacctacggc gtgaaaccgg gtctggtggt
    20821 tggtttgatt gctggaggcg aatatgccat tcagcacgcg gtggaaggag ctgaagatag
    20881 ccgggaaggt ggtgttaacg atctgaaaaa tattaattta acggcacagg atgtggtggt
    20941 tggcattgct gccagcggtc gcacgccgta tgtgattgcc ggactggaat acgcacgcca
    21001 gctcggctgc cgcacagtgg gaatttcctg taatccgggg agcgccgttt caaccaccgc
    21061 tgagtttgcc attacaccga ttgtaggtgc cgaagttgtt accggttctt cgcggatgaa
    21121 agcaggtaca gcgcagaaac tggtgctcaa tatgctttcc accgggctga tgattaaatc
    21181 cggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt
    21241 gcgccaggtc aatatcgtta aaaacgccac cggatgtaat gccgaacaag cggaagcggc
    21301 gttaattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga aaaatctcga
    21361 tgccgcagaa gctaaaaaac gcctggatca acacggcggc tttattcgtc aggttttaga
    21421 caaggaataa cccatggcca aagagatcag cagtgaactt ctgaacacca ttcttacccg
    21481 tgtcggcgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg
    21541 tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt
    21601 cattttgacc agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc
    21661 aaaagcgatg agcgagctgc tgggcgatgc accggtacag gatgccgcag aaatcgccgc
    21721 ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat ttctcgccaa
    21781 attcgccacc atcttcacgc cgctgatccc cggttttatt gccgccggtc tgctgctggg
    21841 gatagcgacg ttaattgcca cggtgatgca cgttccggca gatgctcagg gcacgctacc
    21901 cgacgcgctg aattttatga aggtgttcag caaaggtttg ttcaccttcc tagtgattct
    21961 ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg caattattgc
    22021 cgcactcttt ttgctcggtt ataaccccgc tgcaaccacc ggttactacg ccggttttca
    22081 cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgc tgattgccgc
    22141 gtgggcctgc gcccgcattg aaggcatggt gcgccgcttt atgccggacg atctcgacat
    22201 gctgctgacc tcgttaatca ccctgctgat caccgctacg cttgcgtacc tgatcattat
    22261 gccgctgggt ggctggttat tcgaaggtat gtcatggctg tttatgcacc tgaacagtaa
    22321 tccgttcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtat ttggcgtgca
    22381 tcagggcttt attcctgttt acctcgcgtt aatggacagc cagggattca acagcttatt
    22441 tcccatcctt tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg
    22501 ggcgcaaccg cacagtgcgc tacgcagtca ggtacgcggg gcgattattc ccggcctgct
    22561 gggcgttggt gaaccgctga tttacggtgt caccctgccc cgcatgaagc cgtttgttac
    22621 cgcctgttta ggcggcgcgg cgggcggttt gtttatcgga ttgatagcct ggtggggtct
    22681 gccgatgggc ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc cgctgatgac
    22741 ttccgcacaa ggcatccttc cggcaatggc ggtttatgct ggcgggattc tggtggcatg
    22801 ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc tggactgata
    22861 ttatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc gtcagcgccg
    22921 caaaataccc tgttctgaca gaaggtagtt gatcaagtat atgcggcgtt aaagcagtaa
    22981 taaaaaggcc agtcagtaat gttactgcct ggcctttaat caatgaatca gaaacgctta
    23041 cagcgccatc aacttgtcca gcgacggtgc gaaataatag ccgccggtta ccggtttggt
    23101 gaaacgcaac atcgcatcac gtttgccatc ggtatcgcca aacatgctca gcagttgctg
    23161 ctcaatgtta tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag tgccactggc
    23221 ggtgccgtac ggcaggctct ggcgaacaat cttcagccct ttgccatctt ctttcagatc
    23281 aacgcgggtg aggtgagagg tttccggacg ttcgtcgccg tcaatctctt cgttggcctc
    23341 tttggtgcgc ccgatcatca tctcctgatc gtgaacgctc atccggttga gctgcttcag
    23401 gttgtgctcc caacgctgca caaacacata gctgccgccg gaatccacgc cgtctttgat
    23461 aaccgccact tcgcggcgcg tctcttcacc cgccgggttt tctgtaccgt caacaaagcc
    23521 gctcaggtca cgctcttcca cccaacggaa gccgtggatc tcttctttca cttcaatgca
    23581 gtcaccaaag gcttccatcg ccgcctgagc gacagagaag tttacgtcgt gacgcagaga
    23641 aagaatgtgg atcaacacat cgaactgggt cgtcggcgca aggcctttac cgtagcccgg
    23701 aaaatctttc agctcttctg ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc
    23761 aaaggcaacc accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt
    23821 gtcggcaaat gttttactgg ccgcacgcag ggcgtcaact tcccctttca cgttggcttc
    23881 gatccaaatc gccgcgcggc aatgttctgg caaaatgcca ctctgaacct gagacattgt
    23941 tcctcctgaa aataagaatg ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt
    24001 tttttcgcga cggcggtttg ttcagacgca aattaacgcc gccagataat tttactgact
    24061 ttccagtttt tcagggtatc gtccgaaggc attaaccctt caggaccacg ccattccccg
    24121 ctgaactggt agctaatatg ttgactgcct tcggctttac attcgacggc acgattatca
    24181 tcaccatcag ctttttggca attgccaaat gctttgctgt aaaggtcgct aaacggtgta
    24241 ccgattttaa caccggtgtc agcaggaata tcgctatcca gcacgtcaat gcgactgatc
    24301 gtgccttgat cgccgttaat caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag
    24361 cgcaccacgt tgccgttcgt ggttttcatt ccgctgcgca ggcgataatc gccatcaagc
    24421 gcatcagcaa tggcttgttc ttgcagtggt gtggacgccg ttaattcacc cacgccctgc
    24481 tcgctcactt tggtggacga tccaaaccag ttccacgggt tagcggcaga ccagttaact
    24541 gacgacatcg tggaacagcc ggtcagcatc aacggcatag cgcataacat taaacgcagc
    24601 gatttcatgt cacttccttt ggttattcaa taacgttgct tggagtgcaa attcaccaaa
    24661 aagtgccgtt attctttttc ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc
    24721 cagattaacg ccacaatatc cgccactacc agcgccagac cgataccgct aacggattca
    24781 ccgttcagcc acagccacgg ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga
    24841 aaatagagca catgccaggt gcgaggaaac gaagcccgcc gaccgctcaa caaaaacgcc
    24901 agcaccgccg gaataccggg aatcagcccc agccagaaat tatcgtgatc gggataaaac
    24961 agatttagca gcgcagtacc ctgctcacgc gacgcaccgg caatgacaaa cagcacccag
    25021 gttcgcgcct gaagcaatag cacaagccag aagagcaagg gtaaacgcag gcgtccgtgc
    25081 gcatcataat ggacaggatg gaactcagta ctcttcatct tcaatcaaac gcttacccag
    25141 actcagcacg tcggcgtgtt catatcccag gcgttcatac attccgagca ccatgtcgtt
    25201 atcttccggc acgttgatct gaattttcgg gcagccgcga gcaatcagct ttttctccag
    25261 ccggttaagc aacgcattgg caattccacg cccacgaaac tctggatgca cgccaagata
    25321 ataagcagac ccgcgatgcc cgtcataacc gcccatcacc gttccgacca cttcaccgtt
    25381 tacctcagcg accaaaaaca aactgacgtc atggttcatc ttacgctcga tgtccatttc
    25441 cggatcgttc cacggacgca gcaagtcgca acgctcccaa agggtgatga cctcttcgaa
    25501 atcttcctgg cgaaatacgc gtatctccat ggtattcgtt acctttttgc gggttaaaag
    25561 gctgattatg gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt
    25621 ggcataatgg ggagttgtca actattgaaa tgaaaagtaa aacaattctc aacagcaaac
    25681 cgtcgtaacg gattacgcga tacgatataa catctggaac tttattatta caactcaggc
    25741 cgtatgagca cttttaaacc actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc
    25801 ggtttagctg ccctgacgtt gtcaggaatg tcgcaagcca ttgccaaaga cgaaccttta
    25861 aaaaccagca acggacacag caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt
    25921 gttctcgatc caggccacgg cggaattgat accggagcga tcggacgcaa cggttcgaaa
    25981 gaaaaacatg tggtgctggc gattgctaaa aacgtccgtt ccattttgcg taatcatggg
    26041 attgatgcgc gtttaacgcg ttctggcgat acgtttatcc cactttacga tcgcgttgaa
    26101 atcgcccata aacatggcgc agatctgttt atgtcaattc atgccgatgg ctttaccaac
    26161 ccgaaagctg ccggtgcttc ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg
    26221 gcgaaatacc tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact
    26281 gacaaggatc acctattgca gcaagtactg tttgatctgg tgcaaaccga tactattaaa
    26341 aacagcctga cgcttggctc gcatattctg aaaaagatta agccggtgca taaactgcac
    26401 agccgcaaca ccgaacaagc cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg
    26461 ctggtggaaa cctcgtttat caccaacccg gaagaagagc ggctgttagg cacggcggcg
    26521 tttcgtcaga aaatcgccac agcgattgct gaaggcgtga tcagttattt ccactggttc
    26581 gacaaccaga aagcacattc gaaaaagcga taagttatga aacccgacgc acaccaggtt
    26641 aaacagtttc tgctcaacct tcaggatacg atttgtcagc agctgaccgc cgtcgatggc
    26701 gcagaatttg tcgaagatag ttggcagcgc gaagctggcg gcggcgggcg cagtcgggtg
    26761 ttgcgtaatg gtggtgtttt cgaacaggcg ggcgtcaact tttctcacgt ccacggtgag
    26821 gcgatgcctg cttccgccac cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg
    26881 atgggcgttt cactggtagt gcatccgcat aacccgtatg ttcccaccag ccacgcgaat
    26941 gtgcgttttt ttattgccga aaaaccgggt gcagagcccg tttggtggtt tggcggcggc
    27001 ttcgatttaa cccctttcta tggttttgaa gaagacgcca ttcactggca ccgcaccgcc
    27061 cgtgacctgt gccagccatt tggtgaagac gtttatcccc gttacaaaaa gtggtgcgac
    27121 gattacttct acctcaaaca tcgcaacgaa cagcgcggca ttggcgggct gttctttgat
    27181 gatctgaaca cgccagattt cgaccactgt tttgccttta tgcaggcggt aggcaaaggc
    27241 tacaccgacg cttatttacc aattgtagag cgacgtaaag cgatggccta cggcgagcgc
    27301 gagcgcaatt ttcagctcta ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat
    27361 cgcggcacgc tgtttggcct gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg
    27421 ccgccactgg tacgctggga gtatgattat cagccaaaag atggcagccc agaagcggcg
    27481 ttaagtgagt ttattaaggt cagggattgg gtgtaactcc ctcaccccca ttcccgcatc
    27541 cgctgatgca acgtcaacga cggcttctcg gcaaacagct gctggtaatc cgtggcaaat
    27601 tgccccagat gccagaatcc ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac
    27661 cacgggctta tcagttcgcg gcgtacgacg ttcaggcgaa tgcgtttcag ccacgcgttt
    27721 gggccaatgc ctaaaatagc gtgaaacgcg ttttgtagcg tgcggcgact gacatgcagt
    27781 tgattacaca aatccagcac cgtcaccggt tcggacatgt tttccagcac atattcacgg
    27841 gcgcgggaaa gcaaccgacg gtaactctga tgactgatgc tttccgccgt caccattggt
    27901 tgcgcttctt ccagcatcgc ccccatcgcc attagcaaat tatcccccag cacttttcgc
    27961 actgcaggct gatggagatt ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca
    28021 aagccccaca gcgcggcttt atgctgctct ttcacttcca gcgccgactg gctacgcagc
    28081 atatgtaata cccgatccgg attgtgcaaa aagttagcct gacaggtgat gacatcttca
    28141 gacagcacta cgcccaggat cgtataatcg tccggcgtac ttaattcaaa ctcggttcca
    28201 ccggggcgcg tcgctatttc tgcgcttccc agacattgcg aaccgataaa tccctgctca
    28261 ccgcgcgtcg ccggaatgcc aaaccagaac gagttcggcc agaccaggca cgactgacgc
    28321 agcgccagac cggtgtattc acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat
    28381 tcaccatgaa acttgcccgg atgcagctga tcgtaaatct gctgctaggc ggtaatcgtc
    28441 aaagcatgtt catagacatc cgttgtctgt cgttgatgaa cattatccac ttcgaccttc
    28501 ggcgtgagct tcaggttttc gggtaagggt tcatgataaa gatggtgcaa attggctgta
    28561 cgggtctttt tcatgatgtt aatgccgggt gttgtaggac acccggcacc tccgacaggt
    28621 taatggggct tgagacgata acgactactg cgtttacgta acgtcccggc agaaaagagc
    28681 tgttcgagcg catttctggc tttttccagc ggccagccaa agtgggcggc cacttctcct
    28741 gccgtcattc cctgacatac tgatgttaac agcgccaaca actcgtcagc agattccgcg
    28801 acgataactg gcgcgtcggg ttcctttacg ggttgcttcg gctggcggtt aaagccagta
    28861 accagccact gggtgtcatc gtctggccga ccgatctcct tacggctgat gacacgcccg
    28921 gtacgcatcg cggcagcaca acctgcgtcc agcgccgcac gacacgccgc cagatcgcct
    28981 tccaccacca gcgttaagcg accagggtca agcacttcgt gactgagcag acgaacgtta
    29041 gctgctttga gcatggcatc cgctgcatct atcgcggcga ccatgccgtc cacttccagc
    29101 aatcccagtg cattgatcat cggcaacctc cgttacgcac gctggattgg attacgcgcg
    29161 atttccagca ctgcatcggt aaaggcgtta caggctgctt tacacgcggc ctggctaccg
    29221 gttaaaaatg ctgccgagta gttggtttca gacggtggcg ggacgtaggt caccagctgt
    29281 acgtcggcag atttcaacgc ggcatcaatg ccgtaggtcg cttccagcgg cggtgccacc
    29341 agatacgcca tcggatcgcc aagcgtgatc ccggcggttg atgaaagata agaaccggta
    29401 cgcgaaacca catgcgccag gaaggcggtg ttttctgcgt cattggccca ctggaaagct
    29461 gcgccatttt caatatgtgc aaccatcgca tccagaccgg cacgcacttc cgccgggttt
    29521 ggcccaccaa gcataatcag cacttcaccg gcagtcggtg acgggccgtg tgccgctccc
    29581 gcatacagcg agcgaccata taccacttcg accatcgctt gtttggtcgc ttcgtcagcc
    29641 gcaatgtagg ttacgtcgtc agaatcggca gaaatcagcc cgaggctacg aatatgcggc
    29701 ggcaatttta gttcacgcgc aaattcagcg ttaacggagg caatcacccg catggcggtt
    29761 accgacggtc gaatcaaatc taaagctggc atgatgcctc cttatcgggt catgttgatg
    29821 ccggatgctt tctgctccag catacgtttc gccaaatcca caatgacggc acagcagcac
    29881 gaaagagaag caaaagtcag ctacctggta cactaggcag cgggtcagca aaggggggca
    29941 aatgcccctt ttttcatgtc agataatcag atcattacct caataaacta ataatcacat
    30001 cgcggttaca accagtttga tagtgatagc tcaaacccag acgtggcgct ggctgcaagc
    30061 aaaataagcc gcaaaaatgc gaccccatgt ttactagccg caaaaatgcg gtcctatttg
    30121 ccagaacctt ttattattca attggttagt gcaagaaaaa gcgtgattac tgttcaagaa
    30181 gtatccagaa aggtcatgat tttgacgctg aaaaaaaata ggtgggcatt tttgcccact
    30241 cactagccgc aaatttgccc actcactagc cgcatttttg cggcccaaag taataaatac
    30301 agagaaataa taaatacaga tcagcgccaa gatttttcgt cttttcggcg gaatcatgag
    30361 actagtgctt acgtcctgat aacctccaga gcagacctta acttctgtgc agcaggacta
    30421 cgctcattgc cgctcggcta ggtcaaatcc tcacgataaa gctgttcgga ttgtgaccat
    30481 aatgttaaag gactctttaa atcattcctt gacgaattaa ctagctggct taagcggaat
    30541 agcatgcagg ataaaaacaa aaccagctta gcagtgggga gagcttcgct tcgctcgtct
    30601 cctggggcca atgctcaatg aaacgacgta aggcacggag cgctcaccat aggtagacac
    30661 ataaacatct gagagcgcac cttctatcgc gctacgcgct caataaaaac caacgacacc
    30721 gccacactgc aagaccaatg gtttttatcg cgtatacaga ccagacagga aacagcagca
    30781 aagcagcgta tagccaggtg cagacagcac actcacacga cgcacagcag gcttaagtga
    30841 tcactcagga aactttatcc ccgccgccga tcaccgcgaa ctatcatgac cgcgccaaat
    30901 tcgaaaggaa tggcccatag aacgcgatga acacacataa gcgctacact tacttaacgt
    30961 cgccacagag cgattgagag cgcttaaaat cgatgcgatt tcattaaatt tttctcactc
    31021 gcctgaatgg tggcgatcag gactgatcgc aacattgccc cgcctcgata ttgagcgcat
    31081 aatagataca tacattaagc ggaaaagaga taagtttatt gtgaaaatca gggggttaca
    31141 tatcatgcgc atatcagaac aacaacggca gatcctcgac tatctggcaa aaaacgaatc
    31201 aatcaccgca cgacaagccg cagatctcgt ttatggtggc aatgttaccc gcgtccaggt
    31261 tgacgccgcc cgccgctcac tcatgacgat ggttagtaat gggctaatca ggaaacaagg
    31321 ccgcgctttc gtagctgaaa gacatatcaa tcacgcttat agccaggacg ttgaagaatt
    31381 gcgccgccag atcctttggg tattcagcaa acagggtcag gagtatttcc tttctgaaca
    31441 cccaaatttc gccgccaaag agttaacagt aagcggattg tcgtgcgcac tggattatct
    31501 tgtcggtgaa ggttgcgtta cttacaagca gctatggcac gccctgaata cgcttgtaga
    31561 agatggttta ctcattgcca cgcgtgagaa catcgaatac tggataatgc cgcgttcagg
    31621 tcgccgcaat catgcacggt atatttcagg caacagatac agaacgccgg actttaatcc
    31681 gacacaggaa gatcgggacc gtgccgaaat gatgtgcagg gaacaggaga aaagaattat
    31741 ggataagtta ttttcgatgc agtgaacaca agcgggcgca atgcccgttt ttattgcctt
    31801 attccacgcc tgacgaatca atttgatcac acctgagaaa atgcatagcc agaagttaag
    31861 gccgttatat tgcgattggg agcgttttaa acggtatcaa tttaattaat tctgatgtgg
    31921 tgatttgcag gcaaaaaaaa cccgccggag aagcgggaaa tagagacata tcgttgtagt
    31981 tattaacaga tcactttgag gtcgagggcg gctaaccctc gctaatatta tcccgctatt
    32041 accttgcggg tgcaaataat cttttattgc tttgatgatt taataaatag cactttttgc
    32101 taacaaaaac cgctgtcaat atatggccaa aaatgtcaac agaaaaaata aggtttataa
    32161 tattaagtat cacatgggta atgggttcag ggaatcggaa tccaccgcct cacggaaatt
    32221 caaaatttgc ttcttctttc ctccgtgggc ggcttctttt tcgtatcgag gtatttatga
    32281 aagcaataac attagaacag ttcgcagcaa tggcgaatgt atcgaccaga acgctgaggc
    32341 gctggctaaa aaaaccatca accggagtcc ctttgccatt taccccacgc ggtactcgcc
    32401 aattattctg ggagcacgaa tgcaaagaat ggttgtacag aacagaacga taataatcac
    32461 gcatcacatt ttgaggtaaa catcatgacc aatatcgaaa gattagaacg ctgtgccgat
    32521 attatgcgac gcagatggat ttatgaccct gaagcgggtt tattgtttag ccgtgaaaca
    32581 aaagacgtta tcaaaggcag catgaccaat aaagggcatt tggttgtaac ggtacatgaa
    32641 ggtaaattca aagtgacact cacctatcag aaagcctgct atgtatacac atacggcgct
    32701 tatgacgaga cattatatga agtgcaccac gttaatttga acaaacagga taaccgcatc
    32761 aagaatattc gcctgatgcc gaaagaaaag caccgccgga tacataaaaa cgtccgcaaa
    32821 cttattcggc tgggccacac acaatggctt caaccgcaat taattgaaca atcactataa
    32881 acacaggagt aaataccatg cattcattag acagttattt tcagcgcacg acagccccta
    32941 aatcagccgc ccaggaacac cgtgaggaat tccatgagaa agtgatgcgt tctgctgatt
    33001 atattgcgga caagttcgtg gaaacggttc gtccacttgt tgatgaagta gctgataaac
    33061 ttcagtctga aatgccggaa gatatggaag gcactgcaaa gcggcgactc atttgcgaat
    33121 taagtcgtcg ttttggcgtg tcaataagcg cttttaaata atcgaaaaaa ggggggaaag
    33181 ataatggacg catttgaggt actggcaaat aaaggcgctg aactggtagc acagcgtgat
    33241 aaaaccgcca acgaagggga acgcgcactg ctaaataaac agattaaagc gatccgtatg
    33301 gcccagttta aattaatcag taatgaagtt atcgaaacaa ttaaaccgca gatcccacaa
    33361 atcgttgctg acgctattaa agccgccgga ctggagaaac gcattgccgg gctaaaaacg
    33421 aacgcagaga aaatggagat gtacaaacag agcggtgctc cgaatcccga tgagtacttc
    33481 tcaccggatg ttgagtttat ggcgcaggtt gaggaacgtc tgggtagctg tctcaccgag
    33541 gaacagcgtc gctattttga tggagtggat agttcggcag gtattgacct gaactcgtat
    33601 ttcggtcgtg aaattgagca ttttgatgcc gccccgtcta tgccggagcc agaaccagaa
    33661 atggccaccg atcctgaagc tgagcaacgg aaacgcgtat tcaatgcgat agggattcgg
    33721 tactgatcgg ataaaagaaa gccgggaatt accccggctg acttaacaat gtgaccaatg
    33781 aacaaatcgg acaagacaaa caggataagc agtctcgaaa caatgttcgg gaactatatt
    33841 agcacaagtc cggagaaaat catcatgttg gcactacgca tcatgcaggg catcgctaag
    33901 accctcgcag aacatgtctt agacctgaaa cattcgccac tgagcaaaca ggccatgaag
    33961 cgccaaacgc ttcgtctgtg ggctgaatat tcgcttggaa ctatcaataa aatcatcgac
    34021 atgaaatcgg ggccgagcaa ccagagcgcg gaggaaatgg agttcatcag gcgtttaatt
    34081 ctcattcgca gagacattca tagccagtta cacagcgtgg gaatcgatat taatgatggg
    34141 acgggcgatt aattttcgga caagtactat gcgcgcgcga ggagctgaac atgaagcaag
    34201 atattgcaga caggctggag atactcgaag gccagcgggc agaagcgaaa caactacgta
    34261 agcaggccag aagagcgcac aggaacaacg aagcagagtt actaaccaaa tacatcagtt
    34321 tcaccaatta ctgcatctac gagtgttaca aggaagatgc agaggactgg ctggattcat
    34381 tgccagaaca gtactgagca gaataacacc acgtcatgtt gttctgaggt gagcatatca
    34441 cctctgttca ggtggccaaa tttagtgtgc cacttcagca tcaccgttga aatcacaata
    34501 acgatttttt tgtaaatatg ctctcacata tgtaggaata gaccaggttg gcattgaaac
    34561 acatctacca tgaccataat aaagtaattt tttgatatgt ttatggtttt atattaatta
    34621 aaatatatct aaatttgata gttttaaaat tgcaaaatcc agattgtgtt tgtaatgttt
    34681 aatctgattg tattgagtgg cgtttattat gaaaaataaa tcaggtaatt cttgttatag
    34741 agaagcagtc gatattcata gctcacttga gctttcattt atattgcata aatatcattt
    34801 gcgtgtagat gagaagaaat ctcgaaatgt attcagcgcc atacgtgatg agctcatcct
    34861 tgatggggat tcacgacaaa acttagctac tttctgtcaa acatggcttg atgatgaaat
    34921 ccgtgagttg cttaatttag ctattgataa aaacatgatt tataaagatg aatatccaca
    34981 aatggcggaa cctgaaggtc gctgtgtgcg tatgctttcc gattcgtgga gtttccccga
    35041 ttcgcgacac accttaggtt gctcaaccat tggttcatct gaagctgcta tgctcggccg
    35101 cttggcgtta aaatggcagt ggtgtaaaaa acgcgaagta caaggtaaat caaccgaacc
    35161 tgatttgcga tcctgtgcaa atatgctggc ataaattctg ccgttactgt gatgtgaaat
    35221 tgtgtgaaat tccacttgat gatcatcacc tgataatgaa tgctaaagag gttttgaagc
    35281 atgtcgatga aaacactatt ggtgtcgtta cgactttagg tattacttat ttttgtaaag
    35341 cagattgcgt tagtgttttt aagataagtg ttaaagttaa tattagtttt tatatttaca
    35401 tcgtaattat atgtttctat tttaaaaatg ttcgagagta taaaaatgtc taatgctaat
    35461 atcgcaccca aaagtaagaa aatgacagtg gggacactcg ctatcatgaa tataactgct
    35521 gtagtaagtc tacgatttat accttctgaa gcagaatacg gcttaggtgc cattttttac
    35581 tatacctttg ctgcaattat gtttttagta cctgttgcct ttgttgcggc tgagctagct
    35641 acaacatatc cacaaaaagg gggggcattt cgatggatag gagaagcatt tggggctcgc
    35701 tggggttttg taggaatgct tatgacctgg ctacaagtca tcccctactt tccaacagta
    35761 ctgacatttg gtgcagtctc ggttgcattt attgatgttg atatgggggt ggcagaatca
    35821 attgccagta ataaatggta tattttattt ttcgtactat ttgtttactg gggagctatt
    35881 tgtgttgcat tacgtggcgt tggtattttc tcgcaggtat ctaagtggtg cggtattata
    35941 gggacaatta ttccagctat tatccttgtt attcttggtt tctcctatct gtggttcagc
    36001 ggtaaaccag cggtgatccc attaggttgg ggtgatcttt tccctaatat agctaacttc
    36061 cagaatattg ttcttgccgc cagcatattt cttgcttatg cagggatgga aatgaatgcg
    36121 gtccatgtta acgatttaga taatccaact aaaaaatacc caattgcaat taccatcgct
    36181 tctataggta ctattgcaat attcctgctc agtactttag gggtcgcctt tattattcct
    36241 acagataaaa ttagtctaac tcaaagtttg ttggttgcct acgatatgct gttcgaatgg
    36301 gctggagtgc catggcttgg ctctgtgatg gcatttatgc tagcaattgg cgtactgggt
    36361 ggggtggtta cttggattgc agggcctaat acaggtgtat tagcgatcgc gaaagctggt
    36421 tatttaccta agtttttcca aaaaaccaat cgtcatggaa tggggcacca tctaatgttt
    36481 gtacaaggta taattgtctc agttctttct gttacttttg tgataatgcc ttcagttcaa
    36541 gcagcttttc agattttatc acagttaaca gttatgctct atttagttat gtatatgctc
    36601 atgttcgcta gtgctatcta tctacgacat tctcaacctg atacaaaacg cccatatcga
    36661 gcccctgctt tatttttctg ggcaacactt ggattcttag ggtcttttct ggcttttgta
    36721 ttaagtttta ttccgcccga gcaaatccca actggtagtc caaccagcta tgttctttat
    36781 ttgttagtat tagttgccct atttttatta attccaatat taatttacgc cttccgaaaa
    36841 tctgactgga aagatccaaa ctctgatttc gaaccatttt cttgggaaaa aagaactagt
    36901 aattaaattt tccataagct acaaaagatg aggttaaata tgtttagtgc tgaatctcca
    36961 attggtgtaa tagactcagg tgtcggtgga ttgacatcgg taaaggaaat aattaatttg
    37021 cttcctggtg aagatattat ctattgtgga gataatatga atgcacctta tggcaaccgt
    37081 tctgccgatg atatcatcag tttgactaaa aaaatgctga cttttttaca gtcaaggaat
    37141 gtcaaactag ttgctgtcgc atgtaatact atatcctcaa ctttggaatc cgaagaatac
    37201 agcggatatg ctaagagctt tccgtttcct atcttatcca tcattgaacc tgcagtagaa
    37261 gatgtgattc gccagcaata taaaaatgtt ggtattatcg ctaccgagtt tactattaaa
    37321 acggggtgtc acaaagagct aataaaaaaa ttgaattcaa caataaatgt atttggagaa
    37381 ccaagcaaaa atcttgcaat gttaattgag gaaggtaatc ttaatgctcc tgcaatcttg
    37441 aatgacatca aaaaacatgt taaccattta atttccctcc atccagtaaa tgaaattatt
    37501 ctcggctgta ctcactatcc aattgttcag aatttttttg aagcagttgc accggatata
    37561 aaatttatta atcctgcaca tgatcaggct atctctatta aaaatcactt gggacagcta
    37621 aatttattaa actcctcaaa tataggaact ttgcaaataa atactagtgg aagtatggag
    37681 atctacaaaa cggtactagg ggaattgagc attacgaaac ctcatacatt cagtatcagg
    37741 cagttttaaa tttatccggt acaccgtaaa atcccgtcct tctgaactgc accccaaaag
    37801 ttggacacca actgagtaag gtgcatttta tggacaaacc cagttacata ctgggtagtg
    37861 catccaatta gtagaacatg tgtttttcga taaacgctcc gatcactttt tcgtggatct
    37921 caactgagcg tgagaagcag attgttttac gacccaaccg cttaatgcgg gtgcgtagcg
    37981 tcagattatt acgctcaatg cgttgggtga atattttgcc ggtcagatgc ttattcttcg
    38041 gcacctcccg gccatagctg ccccagtcat cgctggtcag catgccgatg ttgaagggtg
    38101 taagcagtgc cagtagctcc cggcacgttt gatcggttcg gggaccaaaa gtgtaggcca
    38161 gtacaccgcc tgttttggtg ttgtacgcgt acccgagcca atgttgccgg gctttactgc
    38221 caacgtagct ccattgctca tcaagctcgc agataaacgc cacatcagca tgggcaacag
    38281 gcgaagaagt tattcgcttt ggcgcgagtt ttttaaagtc cggatgacgg tgttactacc
    38341 aattttcagt gtcctggcgg tatcgcgaac cccggcacca ttaaaggcca tttcagttat
    38401 cagctctttc atacccggct tacgtgcttg ataagtataa gtgaactgaa acacacggtg
    38461 gcagtcacgg cagcgaagtc tgtcacggcc tttagggttc tgaccatggc ggtaaacctg
    38521 agctgactga caacggggac aatgaatatt aacgctggtc atgagataac ctcaaaagcc
    38581 cgtattatac atcagattca actaattaga ggcatcaccc atccagcatt ataaaacata
    38641 ctaacaataa ctaaattata ataatcacaa cttacaatca aataataaat gcataagatt
    38701 aacaatgatt ttttcgggtt atatttatct aaaaaagcag aacattatgt aacttattat
    38761 tttctaagcg aaaatttatt gtgcggtcag actaacaaac ttgcgtacca cccgaagaac
    38821 atgcgaagat ttaagtattc tttctttttt ggtttttatt tttcattttc tcttttcttc
    38881 tctttccggt tctgttctgg ttttggtttt ttccttttta tttttattct ttcttcaggg
    38941 ttatgctaaa acaccaaaac atttttcacc acaaaagcat ccaatcatct tgccagacca
    39001 atcaccttaa ccggatacac tgccggatat aatcccacta acacccagac accaagcacc
    39061 atacctgata actaccgtat tcaggaagga aatgcgagtg atttcaccgt caccggcaca
    39121 accgatccgc caaaaagagg cgtaccaatg tcgatatagt cccccgcgcg gacaatcact
    39181 tcgtcaatga ctgccaacgg gagttgttgt agctgtgggc gcaacaacat gcccagagct
    39241 ttaccaaagt cctgcccggc caccaccagc aggggatgcg gattcggaaa acgcgcgacg
    39301 aaatcgacca gcgcgttgat gaccgtcaga accgcggcgt aacgcacagg cagcgaggcg
    39361 ggaagcgcca gcacgtacac gtcagttttg ggatcaagat ccagctgaag cagcgcctgt
    39421 tgccaggcac tcaccagatc cgtttcatca atcgggatcg ccaccggcaa attgcgcagc
    39481 ggcagttgta cgccctccag ccagattgtg ctgccagaga gcgaaagggt atgtgcaccc
    39541 gcgccaatca ccgtggagcg tacggtttgc gccggaaact gcacattcat ctcacgcagg
    39601 cgcggatggt catgcagcgc cgttgccaga agtgggccaa tatcggcaaa acagaacggg
    39661 ttggcgggct ggtggcgata acattcgccc acgcctccag aaagcgtaat gatttcgggc
    39721 gtaacacctg cgggcagcaa accggtttgc atcaatgcct gcgcgagcgg cgaaagcgtt
    39781 ccgtcaatca cttcgacaat cagcgccgcc atccgccggg taacctgcac cagctgcgcg
    39841 ccggtcagcg aacgggcgtc agtgcctgca ccgaagcact catccacaat catctgcccc
    39901 ggtttatgag cgtaaaccac gcgcccctgg ctgtcggttt ccagcaggcg accaccgacg
    39961 ttgagacagg cagtgccgct gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca
    40021 ccgccgatat cgatattcag tacccgacac agccgttgtt cagaaagggt ttgcgccccg
    40081 gctccgtgac cggcgatcac ggattcgagg tgcggcccgg cgctggcgac gacaaaatcg
    40141 cccagcgact gtttttcctt catcactttt tccatcagct gtgcgaccag gctttcggta
    40201 aactggcctt ccggcagctg ggcaatgatg gtttcgcgga tgcgctggct ttcggttttg
    40261 tcggctggca cgctggcggg cacggacgcc gttaccggga tcgattcatc acattcggtg
    40321 atggtaaaac ccagtagatc cgccacttca cgggcttccg gggtaataat gctggcgcgc
    40381 agaaccacgg acattgcctg ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg
    40441 atgagttttt tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg
    40501 ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga aacccgtctc
    40561 aagcaataac tttcgcgttg cggcgacgtc agcatctggc atgtccgttt tactgatgac
    40621 ggcgatttgc cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg
    40681 gtcatttgcg ccatggacat agatcagcat gtctacatcc tgtagcgtgg taattaaggc
    40741 gtgataccag cggggatggc taaaatattc acccggcgta tcaatatcgc ccttatcatt
    40801 aaattccacg gccagtgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa
    40861 cgttgttttt cctgccccga cgctacccac aaacgcaatt cgtttcatga atgcctcgta
    40921 ttaacttttg gtcatttcac aaagcgtata atttaataag cgacctaaac cgctgacggt
    40981 ttgtgataac gcttcttcta ctgcgcccac cgagccataa atcaccaatg caccgctgaa
    41041 gcggtcgaga aaaccgatat gcacatcagc ggctttcagc gctaaatcac cggcgatcat
    41101 cgccgtttcg ccgggagtta gcgtcataat gccaattgcg cccgcatccg gaacgccgat
    41161 tttcttcgcc agttcctcac cgggatgcgc gatgagatgc gccagtgtga cctgtttgcc
    41221 gggcacaaat tcctgaatga tgcgttcttt atccatgagt cgccgcctgt gaaaaacttt
    41281 cgtgtatcgt gacaattttt cgtcagagta aataacaaaa ttcggcaagc gcgtttaaaa
    41341 ggtgaggtag atcaataaga atggagagat atttactgtt atgaataaac tttcatatgc
    41401 gacgcatgaa tataaataaa ataatatcga gatattttat atactgattt ttttattatt
    41461 cgcaaactca gtaataagct ttgctaatat atttcaggaa aaccgcttac cctgaatatt
    41521 cagggtaagc gtgagagtta agaatgaatt acagcggttg ggtttgcgct tcaaccacgg
    41581 ccagcgccac catattgacg atacgacgca ccgatgcgat cggcgttaac acgtgaaccg
    41641 gtttcgccac gcccatcagc accggaccga cagtcacacc ttccgagctg gaaacgcgca
    41701 gtaagttgta actaatgcgg gcagcttcca tgttcggcat caccagaata ttggcagaac
    41761 ctttcaaagg gctgtccggc atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc
    41821 cgtgcatttc accatcaatc atcagatctg gcgcacgatc cctgaccagt tccagcgcct
    41881 gacgcatttt gctcgacgac gggcagtcag aagaaccaaa gttggagtgc gacaacagag
    41941 caacgcgcgg ctcaatacca aaacgacgga cagtttctgc cgccatcaag gtgatctccg
    42001 ccagctcttc tgcgtctggt tcgtcattga cgtaggtatc ggcaataaag gtgttaccac
    42061 tcggcagcag cagcgcattc attgcacctg cggtgtgaac gccatcgcga taaccaaaga
    42121 catttttcac cacactaaaa tgttcatgat aatcgcccac cgtaccgcag atcattgcat
    42181 cggcttcccc acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcagcgcac
    42241 gctgcgcctg ttcctgagtg acaccgcgac gcttcatgat ctggaagtat tcggtccagt
    42301 actctttaaa gcgcggatcg gattcgttat tgacgatttc aaaatcaacg cccgctttga
    42361 tctgcaagcc cagtttctga atgcgcattt cgatcacgtt cggacgaccg ataaggatcg
    42421 gtttcgccag tcccagcgtt accagttcct gagtcgcatg cagaacgcgc gcctcttccc
    42481 cttccggcag aactacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa
    42541 acaggttggt tttgtaaacg aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa
    42601 tcggacgcgt cgccacgccc gactccatcg ccgcttttgc gaccgcagga gcgatcttaa
    42661 cgatcaagcg cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct
    42721 gaacgccata cgctgaagcc accacttcac tctgttctgc atgggccagt tctgcaatcg
    42781 cgcgtaccgc cgccagtttc atctcttcgt tgatggcggt tgcgccaacg tccagcgcgc
    42841 cacggaagat gaacgggaag caaagaacgt tgttcacctg gttcgggtag tcggaacggc
    42901 cggtacagat gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt
    42961 ccgggttcgc cagcgccagg atcattggtg cccgagccat tttcttcacc atttcctggg
    43021 tcagcacttt cgggccggaa cagcccagga aaatatccgc accttcaatc acatcatcga
    43081 gggtacgttt gccgtcatcc accaccgcat atgcggcttt ggtttccgcc atatttggct
    43141 cacggccctg atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagcc
    43201 ccagcgctac cagcaggttc atacaggcga ttgctgcggc acccgcgccg gaaaccacca
    43261 tccgcacgtc ggagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc
    43321 tgatgattgc cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca
    43381 gtttctgttc aatatagaaa cattctggcg ctttaatgtc ttcgaggttg atgccgccga
    43441 aggtcggttc gagcgcggcg acgacttcaa taaatttgtc cgggtcgagt tcatcaactt
    43501 caatgtcaaa tacatcaatc ccagcgaatt tcttaaatag aacgcccttg ccttccatca
    43561 ccggtttgcc tgccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga
    43621 tcaccgcaac caggttaccg cgcgcggtat atttgtaggc ttttagcggg tctttttcga
    43681 tttcaagaca aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg
    43741 ccagaggctt ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa
    43801 gtgcactttg ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat
    43861 aaagtgtgtg ggcaacagcc cctgcccaca acgtggcgca cattattacc ctgccggagt
    43921 ctacagactt tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct
    43981 ataacactga tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg
    44041 atgcgtttag cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt
    44101 tcaaggagta tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc
    44161 agacagctgc gatattgagt ccattcgcca ttatcatccc caggatgcca ccactaatcc
    44221 ttcgctgtta ctcaaggctg ccggattatc acaatatgag catttaatag acgatgctat
    44281 cgcctggggt aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa
    44341 actggcggtc aatttcggtg ctgaaattct caaaatcgta cccggttgcg tgtcaacaga
    44401 agttgatgca cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt
    44461 ggacttgtat cagcaacaag gcgttgagaa atcacgcatt ttgatcaaac tggcttcgac
    44521 ctgggaagga attcgcgcgg ccgaagagct ggaaaaagaa ggtattaact gcaacctgac
    44581 gctgctgttt tcttttaccc aggcgcgggc ctgtgcggaa gcaggcgttt ttctgatttc
    44641 gccgtttgta gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt
    44701 ggtggaagaa gatccgggcg ttaaatcggt gcgcaatatc tacgactact ataagcaaca
    44761 ccactatgaa accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt
    44821 aaccggctgc gatcgactga ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt
    44881 ttctccagtg gtacgtaaat taatcccgcc ttctcagacg ttcccacgcc cagcacccat
    44941 gagcgaagcg gagttccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc
    45001 tgaaggcatt cgtctgttcg ctgttgatca acgcaaactg gaagatcttc ttgccgccaa
    45061 actataaacc agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg
    45121 cactcagtat ggatgctgta caaaaagcca attctggtca tcccggcgcg ccgatgggca
    45181 tggctgatat tgccgaagtg ctgtggaacg attttcttaa acataatcct accgacccaa
    45241 cctggtatga tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca
    45301 gtttgctaca tctgaccggt tacgacctgc cgctggaaga actgaagaac ttccgtcagt
    45361 tgcattcgaa aaccccaggc cacccggaga ttggctatac gccaggcgtt gaaaccacca
    45421 ccggcccgct tggacaaggt ttggcgaacg ccgtcgggct ggcgatagca gagcgtacac
    45481 tggcggcgca gtttaaccag ccagaccatg agatcgtcga tcacttcacc tatgtgttta
    45541 tgggcgacgg ctgcctgatg gaaggtattt cccacgaagt ctgttcgctg gcaggcacgc
    45601 tgggactagg caagctgatt ggtttttacg atcacaacgg tatttccatc gacggtgaaa
    45661 cagaaggctg gtttaccgac gatacggcaa aacgttttga agcctatcac tggcatgtga
    45721 tccatgaaat cgacggtcac gatccgcagg cggtgaagga agcgatcctt gaagcgcaaa
    45781 gcgtgaaaga taagccgtcg ctgattatct gccgtacggt gattggcttt ggttcgccga
    45841 ataaagcagg taaggaagag gcgcacggcg caccactggg ggaagaagaa gtggcgctgg
    45901 cacggcaaaa actgggctgg caccatccgc catttgagat ccctaaagag atttatcacg
    45961 cctgggatgc ccgtgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg
    46021 cctataaaaa ggctcatccg caactggcag aagagtttcc ccgacggatg agcggtggtt
    46081 taccgaagga ctgggagaaa acgactcaga aatatatcaa tgagttacag gcaaatccgg
    46141 cgaaaatcgc tacccgtaag gcttcgcaaa atacgcttaa cgcttacggg ccgatgctgc
    46201 ctgagttgct cggcggttcg gcggatctgg ctcccagcaa cctgaccatc tggaaaggtt
    46261 ctgtttcgct gaaggaagat ccggcgggca actacattca ctacggggtg cgtgaatttg
    46321 gcatgaccgc tatcgccaac ggcatcgcgc accacggcgg ctttgtgccg tataccgcga
    46381 cgttcctgat gtttgttgaa tacgcccgta acgccgcgcg gatggcggca ctgatgaaag
    46441 cgcggcagat tatggtttat acccacgact caattggcct gggcgaagat ggtccgacgc
    46501 accaggctgt tgagcaactg gccagcctgc gcttaacgcc aaatttcagc acctggcgac
    46561 cgtgcgatca gatggaagcg gcggtgggct ggaagctggc ggttgagcgc cacaacggac
    46621 cgacggcact gatcctctca aggcagaatc tggcccaggt ggaacgtacg ccggatcagg
    46681 ttaaagagat tgctcgtggc ggttatgtgc tgaaagacag cggcggtaag ccagatatta
    46741 ttctgattgc caccggttca gagatggaaa ttaccctgca agcggcagag aaattagcag
    46801 gagaaggtcg caatgtacgc gtagtttccc tgccctcgac cgatattttc gacgcccagg
    46861 atgaggaata tcgggagtcg gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag
    46921 caggtattgc cgattactgg tacaagtatg ttggtctgaa aggggcaatt gtcgggatga
    46981 cgggttacgg ggaatctgct ccggcggata agctgttccc gttctttggc tttaccgccg
    47041 agaatattgt ggcaaaagcg cataaggtgc tgggagtgaa aggtgcctga tggtgatcgc
    47101 cggatgctgt atgttcccct caccctaacc ctctccccag aagggcgagg ggacggtccg
    47161 tgcgcttttt agggccgcat ctgcgatttt gtttaacggg tgatccacaa cgtgggcctg
    47221 gcgtctggcc catgccagtt atcgcaggta ggttctgcgg cgtaacgcac caggcgaaaa
    47281 cgctgaccgt caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc
    47341 gctaaggtca ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca
    47401 ttcgtctcct ggccgttgtt gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc
    47461 tttttacgcg acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagcta
    47521 atcatcatca gtgctttatc atcggtcagc gcagtgacat tcacctcacg acgtaacgga
    47581 tcaagcgagc agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttacgt
    47641 tcttcgagtg agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc
    47701 gcgggcggta cgctgagagg cggttcgtcc cctttcttga tccacgcggt ttcactgcca
    47761 acgcgctttt gctgagcatc aataaacaac aacgccgctt ttaagccgct cagagaaatg
    47821 gtctgattgc cgtcgcgtaa ggtgatcgcc ttcccttcct gaattatctg caaaaacgcg
    47881 gtgatggttg ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca
    47941 cttaatgcca acggctcacc atctaatagc agccgtggcg ctatctcccc ttcagacgca
    48001 tccggcgact tcaacccacc gcgctcaata cgtaaaacgg catcggtatg cgccccagcg
    48061 ctgcggctca gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacacaaaaa
    48121 ttttgattat tacaggtgac ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac
    48181 accaaagacg tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata gcgcatggac
    48241 ggtacgaccc cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg
    48301 cggctcaatc ggatttgtcg gattgagcca ataataaaca acgaaatatt attgctattt
    48361 ttcagtccat taaatgtgac atttgcaaat agttaagcaa taacaccgtc ttaccgtcac
    48421 gtatctcgcc ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa
    48481 tatcttcatc ttcaacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga
    48541 taaaaaagtg gattagctca gtcacaccgc caggcgacat atacagttca aataatttgc
    48601 gcacttcgcc cacttcatag cccgtctctt caatcgcttc tttacgaatg cacacttccg
    48661 gttcgtcgtt atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat
    48721 taacccaggt agcgacacgg aactgacgaa tcagaaccac gctctttttc ttcgcgttgt
    48781 acaggaggat cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt
    48841 cgccatcttt gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga
    48901 gaattttgtc tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga
    48961 acaattatat tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact
    49021 gattttaccc ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg
    49081 gtaaccacca gatccgcacc acgcacggcg tcgccaccgg cgaagatttt cggattggtg
    49141 gtctggtaac ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt
    49201 acaccgtgcg actccagcca cggcatcccg tgtggattga agccaaacgc cataatcacc
    49261 gcatcggctg gcatgacaaa ttcactgccc tcaatcggta caggtcgccg acgcccctgg
    49321 gcatccggct cgccaagacg cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca
    49381 ttcagctcaa gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg
    49441 ttcttcacct ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg
    49501 acgttactcg cgccgtggcg cagcgcagta cgcacacagt ccatcgcggt gtcgccgccg
    49561 cccagtacca cgacgttaag tccggcggta ttgataaacg gctcttccgg tagctcttcg
    49621 agccccatca cctgtttagt gttggcaatg aggaacggca gcgcgtcata aacgcccggc
    49681 gcatcttcat tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc
    49741 gcgtcgtatt gttccaaaag caaatccaaa gagacatctt tacccacttc acaattgagt
    49801 tcgaagtgaa tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc
    49861 agtttgaaag aaggaatgcc gaaagtgagc aagccaccga tttctggatg gcgatcgtac
    49921 accgtcaccg ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccggacct
    49981 gccccgataa tcgccacccg cttgtccact ttggtgacat gacttaagtc aggacgccaa
    50041 cctttcgcca acgcctgatc tgaaatatag cgttcaatgt tgccgatagt taccgcgccg
    50101 tgctcatcgc gaatagtaca ggcaccttca cacaaacggt cttgcggaca aacgcgtccg
    50161 gtaatttccg gcagggtgtt ggtctggtga gaaagctcga cggcggcgtc gatgtttccg
    50221 gctttcacca gttcaatcca ctgcggaata tggttatgca gcgggcaggt ccattcacaa
    50281 acgctgtgct cgccgaactt aaggcagcgc gaggcttccc gttgtgcctg gtcggcgcga
    50341 aatggcagat aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatccggc
    50401 tcgccacgcg cgggcgttgc ctgcatttgt tcgactttac tcattaccgg catttcttgt
    50461 gcagcggtac tggcatgcca cggttgatgc tcctgacgtg cggtgcgcaa gcggcgggat
    50521 ttcgccatgc cggagagtgc gacgtcagtg accagttgca gcgcgtccgc cgggcaattc
    50581 tcgacacagg caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct
    50641 tttacttttc ctgccgcgac gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc
    50701 accacgcagg atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg
    50761 tggctgattg cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag
    50821 gtcactgcac tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt
    50881 tggctcagga catgttgctc atcattgtga gccatgacac aggcgatttc acaagcatga
    50941 caacccagac attgctgact gttggccata ataaaacgat tcataacgac cttctttttt
    51001 ggttgtaaaa accttattct ttatatgagt gttgttatta cccgacttac aggggaaccg
    51061 gcaatgttca tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata
    51121 atcccatcac tgactaaatt gcgtttcagc gaactggaac attaatgatt ttttgtggag
    51181 aagacgcgtg tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac
    51241 attgtgctgc tgtcgattct ttccacgggt atcgctctgc taactctggc gagcagtttg
    51301 cgcgacgctg aggctatcaa tattgccgga tcgctgcgta tgcagagtta ccgcctgggc
    51361 tacgacttgc aaagtggcag tccacaactc aatgcacatc gccagttgtt tcagcaggca
    51421 ctgcattcac cggtattaac caacctcaac gtctggtatg tgccagaagc agtaaaaact
    51481 cgctatgcgc atctgaatgc caactggctg gagatgaata atcggctcag caagggcgat
    51541 ttgccgtggt atcaggccaa tattaataat tatgttaatc agatagacct gttcgtgctg
    51601 gctttacagc actacgctga acgcaaaatg ctgctggtgg tggcgatttc cctggctggc
    51661 ggtatcggta ttttcacgct ggtctttttt actttgcgcc gcatacgcca tcaggtggtt
    51721 gccccgctga atcagctggt taccgccagt cagcgtattg aacacgggca gttcgactcg
    51781 ccgccgctgg ataccagtct gccgaatgag cttggtctgc ttgcaaaaac ctttaaccaa
    51841 atgtcgagcg agctgcataa attgtacctt tcgctggagg cgtcagtaga agaaaagacc
    51901 cgcgatctcc acgagaccaa gcgtcgtctg gaggtgttgt atcagtgcag tgttcccagg
    51961 cgctgaacac cagccagatt gatgtgcatt gtttccgcca tattttgcag attgttcgcg
    52021 acaatgaagc agctgaatat ctggagctga acgttgggga taactggcgg attagcgaag
    52081 ggaagccaaa ccctgagctg ccaatgcaga ttttaccggt gacaatgcaa gagacggttt
    52141 acggcgaact gcactggcaa aatagtcacg tttcatcatc agaaccgctg cttaacagtg
    52201 tttcatcgat gctgggacgc ggtttgtact ttaatcaggc gcagaagcat tttcagcagt
    52261 tattgttgat ggaagaacgt gcgaccatcg cccgcgaatt gcacgactcg ctggctcagg
    52321 tgctttctta cttacgtatc cagttgacgt tactgaagcg ttcgataccg gaagataacg
    52381 ccaccgcaca aagtatcatg gccgattttt cccaggcgtt gaatgatgct tatcggcagt
    52441 tacgcgagct gttgactact ttccgcctga cgctgcagca ggcggatctc ccctccgcgt
    52501 tgagggaaat gctggatacg ttacaaaatc aaaccagcgc taaactgacc ctcgactgcc
    52561 gtctgccaac cctggcactg gatgcgcaaa tgcaggtgca tttgttgcaa attattcgcg
    52621 aagcggtgct gaatgcgatg aagcacgcca acgccagcga aatcgccgtc agttgcgtca
    52681 ccgcgccgga cggcaatcac acggtttata tccgtgataa cgggattggt atcggtgaac
    52741 cgaaagaacc cgaaggtcat tatggactga atatcatgcg cgaacgcgcg gaacggctgg
    52801 gcgggacgct gactttttca caaccttccg gcggcggcac gttagtgagt attagctttc
    52861 gctccgcaga gggtgagggg aaggggaaca cgccggctga ggtgtaaata attaccctca
    52921 caccggccct ctccctttaa aggcgagagg gaaaaacgtg cccatattga cctgtaagcg
    52981 agtgtgtcag caatctcatg ataatttaca ttaactcctt tttttctcca cgattggctc
    53041 gtaccttgcc gctacagtga agcaagtcaa gcctacaaca atacgcagaa acacgaggtc
    53101 ctcttttaat ggcgaatttc tttattgatc gccccatttt tgcctgggtg ctgacaatcc
    53161 tgttgtgtct gacaggtacc ctggcgattt tttcattgcc cgttgaacaa taccccgatc
    53221 tcgcgccacc gaatgtgcga gtgaccgcta actatcccgg cgcatcggcc cagacgctgg
    53281 aaaacaccgt gacccaggtt atcgagcaaa atatgaccgg cctcgataat ctgatgtata
    53341 tgtcatctca gagcagtggc accggtcagg catctgtcac tttaagtttt aaagcaggca
    53401 ccgatccgga cgaagccgtg cagcaagtac aaaaccagct gcaatcagcc atgcgaaagt
    53461 taccgcaggc ggtgcaaaat cagggcgtga cggtgcgtaa aaccggcgat accaacattc
    53521 tgaccattgc cttcgtctct accgatggct cgatggataa acaggatatt gctgattatg
    53581 ttgccagtaa tattcaggac ccgttaagcc gcgtgaatgg cgtcggggat atcgatgcct
    53641 atggttcgca atattccatg cgtatctggc tggacccggc gaaactcaac agtttccaga
    53701 tgacggctaa agatgtcact gatgccattg agtcacagaa cgcgcagatt gcggttgggc
    53761 aacttggtgg tacaccttcc gtcgataagc aggcgctcaa cgccaccatt aacgcccagt
    53821 cactgctgca aacaccagaa cagttccgcg atatcacctt gcgggtgaat caggacggct
    53881 cagaggtaag gctgggcgat gtcgccaccg tcgaaatggg ggcggagaaa tacgattatc
    53941 ttagccgctt caatggtaag ccagcctccg ggttgggggt aaaactggcc tccagcgcaa
    54001 acgaaatggc gacagcggag ctggtgctca atcgtctcga cgagctggcg cagtatttcc
    54061 cgcacggact ggaatacaag gtggcgtatg aaaccacctc gtttgttaaa gcctccattg
    54121 aagacgtggt gaaaacgctg ctggaagcta tcgctctggt tttcctcgtt atgtatctgt
    54181 tcctgcaaaa cttccgcgcc acgctgatac ccactatcgc cgtgccggtg gtgttgatgg
    54241 gaaccttctc cgtactttac gccttcggtt acagcgtcaa caccttaacc atgttcgcga
    54301 tggtgctggc gatcggtctg ctggtggatg acgccatcgt ggtggtggaa aacgtcgaac
    54361 gtattatgag tgaggaagga ctcactcctc gcgaagccac acgtaaatcg atggggcaga
    54421 tccagggggc actggtcggg attgcgatgg tgctttcggc ggtatttgta ccaatggcct
    54481 tcttcggcgg caccaccggt gccatctatc gccagttctc tattaccatt gttgcggcga
    54541 tggtgctgtc agtactggta gcgatgctcc tcactccggc tctgtgtgcc acgctactta
    54601 agccactgaa aaaaggtgag catcacgggc aaaaaggttt ttttgcctgg tttaaccaga
    54661 tgtttaaccg caacgccgaa cgctacgaaa aaggggtggc gaaaattctc caccgtagcc
    54721 tgcgctggat tgtgatttat gtcctgctgc ttggcggcat ggtgttcctg ttcctgcgtt
    54781 tgccgacgtc gttcttaccg ctggaagacc ggggcatgtt tactacctcg gtacaattgc
    54841 ccagcggttc aacccaacaa cagaccctga aagtcgttga gcaaatcgag aaatattact
    54901 tcacccatga aaaagacaac atcatgtcgg tgtttgccac cgttggttct ggccctgggg
    54961 gtaacgggca aaacgtggcg agaatgttta tccgcctgaa agactggagc gaacgcgaca
    55021 gtaagaccgg cacctcgttt gccattatcg agcgtgcaac gaaggcgttt aaccaaatta
    55081 aagaagctcg cgttatcgcc agcagcccac cagcaattag cggtcttggt agttctgcag
    55141 gttttgatat ggagttgcag gaccacgctg gagcgggtca cgatgcgctg atggcagcac
    55201 gtaatcagtt gctggcgctg gcggcggaaa acccggagct aacccgtgtg cgccataacg
    55261 gcctcgatga cagtccgcag ttgcagattg atatcgacca gcgtaaagct caggcgctgg
    55321 gcgttgatat cgacgatatt aacgacacac tgcaaaccgc ctggggttcg agctatgtga
    55381 atgactttat ggatcgcggt cgcgtgaaga aagtctatgt gcaggcagct gcgccgtatc
    55441 gcatgctgcc agatgacatc aatctctggt atgtccgaaa taaagatggc ggcatggtgc
    55501 ccttctctgc tttcgcgacc tcacgctggg aaacaggctc gccgcgtctg gaacgctata
    55561 acggttattc tgcggttgag attgttgggg aagccgcacc gggggtcagt accggtacgg
    55621 cgatggatat tatggaatcg ttagtgaagc agctgccaaa cggctttggt ctggagtgga
    55681 cggcgatgtc gtatcaggag cggctttccg gcgcgcaggc tccggcgctg tacgccattt
    55741 ccttgctggt ggtattcctg tgtctggcgg cattgtatga aagctggtcg gtaccgttct
    55801 cggtaatgct ggtcgtgccg ctgggggtaa tcggcgcgct gctggcaacc tggatgcgcg
    55861 ggctggaaaa cgacgtttac ttccaggtgg gcctgttaac ggtcattggt ttatcggcga
    55921 aaaacgccat cctgatcgtc gagtttgcta acgagatgaa ccaaaaaggc catgacctgt
    55981 ttgaagcgac gctccacgcc tgccgtcagc gtttacgccc gattctgatg acctcgctgg
    56041 catttatctt cggcgtattg ccaatggcaa ccagcacggg tgccggttcc ggtggtcagc
    56101 atgcggtggg tactggcgta atgggcggga tgatttcagc tactattctg gctatttact
    56161 tcgtgccgct gttctttgtg ctggtgcgcc gccgcttccc gctgaagccg cgcccggaat
    56221 aagcaataaa aaaggcgaca tgccaatgtg tcgccttttt caactttccg attaagaacc
    56281 tgctcagcgg gttcttgctg tttatacttt gtctcaggaa ttacttacga agcataactt
    56341 cgataaagtc tttccagttc cccagttcac gttcaatcat aacaacctct cttataatta
    56401 tgggtattct acggaaacaa tataccgtgg tgaagctaat ttactcgatt gctgcgatga
    56461 ctacctccgg gggacaaacc ttatgtaaat actatggtcc tgcggtgata atttgtatgt
    56521 gatacacagc aacatttcga gatattcata cggcatctaa tactgattta attctggtta
    56581 aaatacagac agataacaag atgaatattc ttaatgttta cgttaaaaat gtttaatatt
    56641 atttaatagt tgttaatttg aatacttcga taatgttata ttttctgata atcatttgca
    56701 ggcaaaatgt tttcaccctt aaatgagtat ttattctcat aaatcgaaaa aggattcatt
    56761 atggttacac tttacggcat caaaaattgt gacaccatta aaaaggctcg ccgttggctg
    56821 gaagccaata acatcgacta tcgttttcat gattaccgcg tcgatgggct ggacagcgaa
    56881 ttattgaacg gttttatcaa cgaattaggc tgggaagcgt tactcaacac ccgtggtaca
    56941 acctggcgta aactggacga aactacccgc aataaaatca ccgatgcggc ctctgcggcg
    57001 gcattaatga ctgaaatgcc tgcaattatc aaacgtccat tgctctgcgc gcctggtaag
    57061 cctatgctgc tgggtttcag tgattccagt tatcagcaat ttttccatga ggtgtagtct
    57121 atgtcgtgcc cggttattga gctgacacaa cagcttattc gccgcccttc cctgagtcct
    57181 gatgacgcag gatgtcaggc tttgttgatt gaacgtttgc aggcgatcgg ttttaccgtt
    57241 gaacgcatgg attttgccga tacgcagaat ttttgggcat ggcgtgggca aggtgaaacg
    57301 ttagcctttg ccgggcatac cgacgtggtg ccgcctggcg acgccgatcg ttggatcaat
    57361 ccgccgtttg aacccaccat tcgtgacggc atgttattcg ggcgcggtgc ggcagatatg
    57421 aaaggctcgc tggcggcgat ggtggtagca gcagaacgtt ttgtcgcaca acatcccaac
    57481 catacggggc gactggcatt tctgatcact tctgatgaag aagccagtgc ccacaacggt
    57541 acggtaaaag tcgtcgaggc gttaatggca cgtaatgagc gtctcgatta ctgcctggtc
    57601 ggcgaaccgt cgagtatcga agtggtaggt gatgtggtga aaaatggtcg tcgcggatcg
    57661 ttaacctgca acctcaccat tcatggcgtt caggggcatg ttgcctaccc acatctggct
    57721 gacaatccgg tacatcgcgc agcacctttc cttaatgaat tagtggctat tgagtgggat
    57781 cagggcaatg aattcttccc ggcgaccagt atgcagattg ccaatattca ggcgggaacg
    57841 ggcagtaaca atgttattcc gggtgaactg tttgtgcagt ttaacttccg cttcagcacc
    57901 gaactgactg atgagatgat caaagcgcag gtacttgccc tgcttgaaaa acatcaactg
    57961 cgctatacgg tggattggtg gctttccggg cagccatttt tgaccgcgcg cggtaaactg
    58021 gtggatgcgg tcgttaacgc ggttgagcac tataatgaaa ttaaaccgca gctactgacc
    58081 acaggcggaa cgtccgacgg gcgctttatt gctcgcatgg gggcgcaggt ggtggaactc
    58141 gggccggtca atgccactat tcataaaatt aatgaatgtg tgaacgctgc cgacctgcag
    58201 ctacttgccc gtatgtatca acgtatcatg gaacagctcg tcgcgtgatg agtggttctg
    58261 caagaggaaa taagcatgga ctggctggct aaatattggt ggattctggt gattgtcttt
    58321 ttggtaggcg tcctgctgaa cgtgattaaa gatctcaagc gcgtcgacca taagaaattt
    58381 ctcgccaaca agccggagct tcccccgcat cgtgatttca acgataagtg ggacgatgac
    58441 gacgactggc cgaaaaagga tcaaccgaag aagtaagctg agtgctcaac cgtaggccgg
    58501 ataaggcgtt aacgccgcat ccggcaatgg tgaacgatcc ctgatgcgac gctcacgcgt
    58561 cttatcaggc caggtaatct gtcttttaca tcatttcaat cacatcatca tcgccaggct
    58621 taccgccact tagcgcttgg tcgaaataat gtttcggaat ggtataacgc aagtgatcca
    58681 gggcaaattg catactgcga ttgtcgatcg catggcctaa atcctccacg atatccagcg
    58741 tcacgtcacc accggcactg attaacgctt cctgcgccgc aactgcatgc gccagatcga
    58801 taaccggatc ttcaccacca tgaatcaagt gaattgtcgt ggctgtagac gcggtctccg
    58861 gcaggctcgc atagcgtccg ttaaaggcaa tgacgcgcga agcaaggccc ggttcggctt
    58921 taatgctctc cagcgccata atcgcgcctt gtgagaaacc aattagcgcc gtggcgttag
    58981 cccctactcc gctctgtttc tgccagtagc gtaccgtttc aataaacgtc ggcatgatgg
    59041 catccacacg cgcctggcga ttatcttccg taataccctg cactgaaaac cactgacgcc
    59101 ccgccggatt accgctcggc tccgcgccac cgacactcac caccagcgca tcaggaaaca
    59161 gcagtgcaaa ccagctgcct atttccccca ttgccaccgg gttatccccg acaccgtgga
    59221 aaagcagtaa caactgttgt gcaggtttat ccgggctttg aacaacaaaa tggtcatgtt
    59281 tcatggcgaa ctccttaact gatgtcatta attttacgcc gctgactcac aatgaccatg
    59341 ggagaaaatt gaaggagtca gtgaaaaaat tgccattgcg ttatgcgatc gcgcagacgc
    59401 tcagtgcgaa cctcatttag tgcgaacagc gcctgcgcgg cctcttcccg ctggcgaacc
    59461 agtaacatct tgcggcctga aagtttaagt gtggtacata actgcgcgtc actggcgttt
    59521 ttctgtaaac gcccacgcag cgccggtaat gccagttcac tggtttgtag cagacgcaat
    59581 aagcaaccta acgatgttaa tagcggacga tgagcgaaag caaaaccggc cagttcaagc
    59641 cagtcgtcgt cagaaaggat ggcatcgttt agtggatcaa caggaagcat ttcgccattc
    59701 cactgcgcga gagcttgcgc atcgcgacgt aaacggtaat gctcacgttc agccagctgt
    59761 ttacccgcat cactcatcgg taacagcgcc atcgccgtat agcaaccgct gctggcttcc
    59821 cgatgattac ccatccgcac cagcacaaaa ccgcagcgtt gccagaaacg ccataactcc
    59881 ccagtgtaac caaaactcac cgaaagatag tcgaggtcat gcgtatattg caaagctcca
    59941 gcaataagtt gtcgccctgt gccttcccgc tgacgagccg gatgaactgc tatccggcta
    60001 acccgccttc cgcgcaatgt cgccgccagt ggattgctgc cgtgcgccgc cagcgactgg
    60061 gccaccagat taccccgcgg gcgacgaaaa cctgcccata ccgcctgact gagttcttga
    60121 gataatccgc cctcatccac cagccacaac gccccggcaa tctcgttttc gccagccgcc
    60181 tgtaaaaaat gttgccctgg tgcatccatc atccggcgta aatccagcgg cgaggtccgg
    60241 tagtgcgcac cagacaataa ctgataaacc tttaacggcg tttctggctc gcttcgccat
    60301 aacgtttgtt caaatgcgga aatgacgata ttgccttgtg gtgtatgggt gaagttttca
    60361 tcgttaaaaa ccagtgcctc actaaccatt ttttccagcg ggcatccctg tgcccagcgg
    60421 atcggctgtt gcagttcaaa acggtgtaaa tgcggaaagc gagcgcaaaa tttcagcaaa
    60481 aaaccacgtc cggtaccttc gtagccctgc accgtagtgg ttaacaacgt tcgaggaaaa
    60541 cgcgatacca gttgatgcaa caacggcgca ggtatggctg cggcttcgtc gaccaccagc
    60601 cagtcggctt gctcatcgct ggctaacaag gcatccggtg caataaagcg aaacttctcg
    60661 cccgcaaatt gtgccagtac atccgttgcc gcttttgcgg gcgcggtgac aatcgcactg
    60721 cccgcaatac gagaaatgag ttgccctgcc aacgccgact taccgcgccc acgcgcagcc
    60781 gttaccgctg ccacgcccgg cggcatggtc agtagctgct gtaagagttg ctgttgttct
    60841 ggttgtggtg cgccggtcgc ggggtgccag tcagtacggg gagtaaaatg cgccaacgag
    60901 aacggctggt tttgccgcca gaggatagcg tcgttatccg ccgtaagtac gcgtttgaga
    60961 tgctggacaa aatgcggcgt cgcaataggg tcagggcaat cactccagcg cagcgagtcg
    61021 gcatcaggtt ggttttccca ctcttcccat acagggagta acaaaaccag ccagcttccc
    61081 gctttcaacg ttccgctaag tgctgcaaag gcagcggcat caaagccgtg gcgggcgtcg
    61141 aataccgcat gccggaactc gcgcccaagt aaagtttgta gcgccgaggg tgaacagtgg
    61201 ttttcagcat ctagctgcgg cgaaatccac agccagtcgc caggtaaggc atcacgcaac
    61261 ttaagagcat gatcaaaaca ccaaccctct tccccgctca acaccagcaa gcggcagatc
    61321 ccttcacgtt tcatttgcgc tgttaatgtg taaagcgcag tcagttcagc catccctgcc
    61381 ccggaaatta aatgctttta ccaaaagtat tgcattgtgc cggatcgccg ctgtcgaaac
    61441 cacgtttaaa ccagctgtag cgttgctgag aagtgccatg tgtgaaactg tctggtacta
    61501 ctcgcccctg actttgctgt tgtaaacggt catcgccgat ggcctgcgcc gcattcagcg
    61561 cctcttccag atcgccagtt tccagaacgc cttgctgctg catactatgc ccccagacac
    61621 cggcaaaaca gtcggcctgg agttccatac gcacagataa gcggtttact tccgcctgcg
    61681 tcgcgttttg ttgcagttga cgaactttcg gctcgatgcc taacagtttc tgcacatgat
    61741 gaccgacttc atgggcgata acgtacccct gggcaaaatc gccatccgcg ccaagtttgt
    61801 ctttcatgtc atcatagaag gagagatcga tataaactgt gccatccgcc gggcaataga
    61861 acggccccat aatggactgg cccgccccgc agccggtgcg cgtcattcca cggtacatga
    61921 ccagcttcgg ttgctgatag gtcttaccca tcttttcgaa ctgttgtccc caggtgtctt
    61981 ccgtggttgc ctgaatcacc gaggtgaatt ttgcggcttc gtcttcatta gggctaattg
    62041 accgcgttga ttgttgttga gaaaccggct gcccggtcat caacccggtt aaatcaacgc
    62101 catagtagcc tgcaaccagc acgactatca gtaaaatcag cccgccttta ccgctcggca
    62161 gacggaaacc gggaccgccc attgacggac cgccagagct gttgcgcctg tcttcaacat
    62221 tgtcactttc acgtcgccct tgccaacgca tagatacctc aacaatatat tcattataga
    62281 aatgatcgta ggtggttcag aggaagatta ccacaggaaa gcgggatgaa atggcggagg
    62341 aatgagcgtt caatgcatat attccaagag cggttgtctt gccgtgccag ctgcacggca
    62401 agatgatgaa cagaaaaatc agtccagctg tacacccagg cggcgggcga cggcttcata
    62461 ggcttcgatc aggccaccga ggctctggcg gaaacggtct ttgtccattt tctccagtgt
    62521 ttctttgtcc cacaggcggc tgccgtccgg ggagaactca tcgcccagta ccacttcacc
    62581 tttgtacaga ccaaactcca gcttgaagtc gaccagaatc agaccagcat catcgaacag
    62641 ttttttcagc acgtcgttcg ctttgtaggt cagctctttc atacgcgcca ggttctcttt
    62701 gctcacccag ccaaaggttt cgcagtaaga ttcgttgacc atcggatcgt gcatggcatc
    62761 gtttttcagg aacagatcga acagcggcgg gttcagatca ataccttctt cgattccaag
    62821 acgtttcact agagagccag cagcacggtt acgcacgaca cactcaaccg gcaccatatc
    62881 cagctttttc accagacatt cggtatcgga gagcagacgc tccatttgag tcgggatacc
    62941 cgcttcagcc agtttgctca taatgaagta gttgaacttg ttgttcacca tacctttgcg
    63001 atcaaactgc tcaatgcgcg cgccatcccc tgctgacgta tcattgcgga attcgagcac
    63061 caacaggtcc gggttttccg tgctgtatac ggttttcgct ttaccacgat acaactcagc
    63121 ttgcttttgc atctttatca ctcctgggtg tgaattaacg ttttaaaatc ttttgctgtc
    63181 tggtgtgccg gatgttttgt cggatgcggc gtgaacgcct tatccgaact acgtccgaca
    63241 aacaaatttc gtgcgaatta ccgctaaaat cgcgttttcc tgccgacgca cacgtttgcg
    63301 tatcatatca gaaaaaaggg ccggatgatt ccagccctgt atttttactt gctaaacgca
    63361 gcctggaaga cagctaccag cgcgtcgttc tgactctgag tcagagtatg acctttcgga
    63421 tcgatgaact gtaggctgct gcggttatct aaatcgccaa cctgcagttt atagtcaccg
    63481 gatgccaggc ctggatcgct cgcgcccagt tcctgccagt cgctgtcaga cagcggctta
    63541 taagttacgg ccatgttgcc ctgcgaacgg gtgctgtcgg tcactttcat gcccactttt
    63601 tccagcgccg ctggcagacg ttgccaaacc acattgaacg gcccgcgtac gaccagcatt
    63661 ggtaaaccgg tgtcatcagc tgcactttgt acgtccatag tggtggaggc acgattttgc
    63721 gcagcgttcg cggcgtcagt ggcagattta tccagaccgg cggaaataac gttcatcatc
    63781 tccgtgctgt aacgctgcat ggaagccgcg tctgcaaccg gtttgcccgc ctgttccagg
    63841 ttcagcagtt taaccgtaac cgcctgctga taaccctgcg gcttaacaga gatttgataa
    63901 cgaccacgat actgctcgtc ttcgtccaga cggttccatt gtacccaatc ggtggtcagt
    63961 gtctgaccag catcatcacg ttgggtgatg gtgtagtttt tcgcctgcag cacgctaacc
    64021 acctgcggcc acagagtatt gccacgacca ttttccacca gcaatgaagc ggtatcgccc
    64081 gtgaactggg tacgcgcgcc agaaaccagt gccaacggct gggctggtgg acgaatgtcc
    64141 agcgccttac cgacagcacc actaccgttg gtcaccggga ttgcataatc accggaggtc
    64201 accggcaaaa tcattccagc cggggcatga agctccgcaa gcggtgccgc ttccaggtag
    64261 gcttcatcac cactgacctg acgcttatag cgtgagtcag aactacaggc agcgagtaat
    64321 aaaacaagcg aaacacccgc aacctttgcc aggcgcgact tttgaacaga gtaagccatc
    64381 aaatctccct aaactttaca gcaaaccggc atgcttaagc gccgctctga ccgtctcacg
    64441 accactgtcg gtgattggtg tcattggcag gcgcagcgta tcggtcgcca caagacccag
    64501 ttccttacat gcccatttca ccgggattgg attgggttcg acaaatagtt tgttgtgtaa
    64561 tggcatcaga cgctgattaa taacgcgtgc ctcggcaaaa tgcccttctg ctgccagttt
    64621 gcacatctgg gccatatcac gcgctgcgac gttagccgta acggaaataa ctccatgacc
    64681 gcccaattgc atgaagtcca gcgcgctcgc atcatcgccg ctcagcagaa caaaatcatc
    64741 tgaaaccagc tctttgatct ggtttacacg cgttaagttc cctgttgcct ctttgattcc
    64801 gataatattt tttactttcg ccagacggcc caccgtttcc gggagcagat cgcagccagt
    64861 acgggacggc acattataca gaatttgcgg caggtcagta tgctcagcga tggctttgaa
    64921 atgctgatac aaaccttctt gcgacggacg attgtagtaa ggggttaccg tcaggcagcc
    64981 gacgatacca ctgtcattga agcgctgcgt caggctaatg gcttccgcag tagcgttagc
    65041 gccggtcccg gcaattaccg gaatgcgccc atcagccaga tccagcgtca tcatcaccac
    65101 atcagcatgt tcgtcatgat ttaaggtagc ggactcgcca gtggtgccaa cagaaacgat
    65161 cgccgaagta ccgctggcga catgataatc aatcagtttt ttcaagctag cccgacagac
    65221 attacctttt tcatccatcg gagtaacaat cgcgacaata cttcccgtga acatgggcca
    65281 tcctctgtgc aaacaagtgt ctcaatggta cgtttggtat ggcattaaaa gcaagcagac
    65341 agaaccgttc tgattgttgt atgcatgatt tttttatgct ttccttaaga acaactcacc
    65401 ccttaaagga ataaccagtt tgacactgtc atcgcaacat tatctggtga tcactgcgtt
    65461 gggtgccgat cgccctggaa ttgtgaacac catcacccgt catgtcagta gttgcggctg
    65521 taatattgaa gacagtcgcc tggcgatgct gggagaagag ttcacgttta tcatgctgct
    65581 ttccggttca tggaatgcca tcactctgat tgaatcaacg ttaccgttga aaggtgccga
    65641 actggatctt ttaatcgtga tgaagcgcac gacggcgcgt ccgcgtccgc caatgccagc
    65701 atctgtctgg gttcaggtcg atgttgcaga ctccccgcat ttaattgaac gcttcacagc
    65761 acttttcgac gcgcatcata tgaacattgc ggagctggtg tcgcgcacac aacctgctga
    65821 aaatgaacgg gctgcgcagt tgcatattca gataaccgcc cacagccccg catctgcgga
    65881 cgcagcaaat attgagcaag cgttcaaagc cctatgtaca gaactcaatg cacaaggcag
    65941 tattaacgtc gtcaattatt cccaacatga tgaacaggat ggagttaagt aatgaatcca
    66001 ctgaaagccg gtgatatcgc accgaaattt agcttgccgg atcaagacgg agaacaagtt
    66061 aatttgaccg acttccaggg acagcgtgtt ctggtttatt tctacccgaa agccatgacc
    66121 cccggctgta ccgtacaggc ctgcggctta cgcgataaca tggatgagtt gaaaaaagcg
    66181 ggcgttgatg tgctgggtat cagcaccgat aaacccgaaa aactctcccg ttttgcggaa
    66241 aaagagctgc ttaactttac gctcctgtct gatgaggacc accaggtgtg cgaacaattc
    66301 ggcgtctggg gtgaaaagtc cttcatgggc aaaacctacg atggtattca tcgcatcagc
    66361 ttcctgattg acgctgatgg caaaatcgaa catgtctttg acgatttcaa aaccagcaat
    66421 caccacgacg ttgtgctgaa ctggctgaaa gaacacgcct gattactttg ctccattccg
    66481 tgctggctgc gcttgcggcc agcatacctc acttctcgtg atcaagatca cattctcgct
    66541 ttcccctgcg acacgggtgt cgaatccatt ttttgctgaa cgttaatgac catcattttt
    66601 gtaccgttca gaatccagtt aatacataac ttattgaata gattgagtta atcagaatgg
    66661 catcctttat acaatatgaa atgcaatgtt tcatatcatt ttcaaggagc cgacatgaac
    66721 cgctttgtgg tggccgaacc actgtggtgt acaggatgta atacctgtct cgctgcctgt
    66781 tcggacgtgc ataaaacgca aggtttacag caacacccgc gcctggccct ggcgaagacg
    66841 tcaacaatca ctgcccctgt cgtgtgtcat cactgtgagg aagccccttg cctgcaggtc
    66901 tgcccggtca atgccatctc tcagagggat gatgcgatcc aactcaacga aagcctctgt
    66961 attggctgca agctttgcgc cgtggtctgc ccatttggcg caatcagcgc ttcaggaagc
    67021 cgtccggtga atgcccatgc gcaatatgtt tttcaggctg aaggctcact caaagacggc
    67081 gaagaaaacg tgccgcccca acatgcgttg ctgcgctggg aaccgggtgt ccagaccgtc
    67141 gcggtgaaat gcgacctgtg tgatttcttg ccagaaggtc cggcctgcgt tcgcgcttgc
    67201 ccgaatcagg cgttacggct gatcaccgac gatagcctgc aacgtcagat gaaagaaaaa
    67261 cagcgccttg ccgcaagctg gtttgccaat ggcggggagt ctcccctttc cctcactcag
    67321 gagcaacact aatggatgcc ctgcaattat taacctggtc gctgatcctc tatctgtttg
    67381 ccagtctggc ttcgctgttt ttactcggtc tggacagact ggctattaag ctttccggca
    67441 tcacatcgct ggtgggcggc gtgattggca tcatcagcgg aattacgcaa ttacatgctg
    67501 gtgtaacttt agtcgcccat tttgccaccc cttttgactt tgccgattta accctgcgaa
    67561 tggatagcct ctcggcattt atggtgctag ttatctcctt gctggtggtg gtttgttcgc
    67621 tctattcatt gacttatatg cgcgaatacg agggcaaagg cgcggcggcg atgggcttct
    67681 ttatgaatct tttcatcgca tcgatggttg ccctgctggt gatggacaac gctttttggt
    67741 tcatcgtgct gtttgaaatg atgtcgctgt cttcctggtt tctggtcatt gccaggcagg
    67801 ataaaacgtc gatcaacgct ggcatgctct acttttttat cgcccacgcc ggatcggtgc
    67861 tgattatgat cgccttcttg ctgatggggc gcgaaagcgg cagcctcgat tttgccagtt
    67921 tcgcacgctt tcactttctc cggggctggc gtcggcggtg tttctgctgg cctttttcgg
    67981 ttttggcgcg aaagccggga tgatgccgtt gcacagctgg ttgccgcgcg ctcaccctgc
    68041 cgcaccatcg cacgcttcgg cgttgatgtc tggcgtaatg gtcaaaatcg gtattttcgg
    68101 catcctgaaa gtagcgatgg atctgctggc gcaaacgggt ttgcctctgt ggtggggcat
    68161 tctggtgatg gcgatcggcg caatctccgc gctcctgggc gtgctatatg cgctggcgga
    68221 acaggatatc aaacggctgc tggcctggag taccgtcgaa aacgtcggca ttattttgct
    68281 ggcagtcggt gtggcgatgg acggtctgtc actgcacgac ccgctgctca ccgtggttgg
    68341 actgctcggc gcactgtttc atctgctcaa ccatgcgctg ttcaaagggc tgctatttct
    68401 cggcgcggac gcgattattt cgcgtttgca tacccacgac atggaaaaaa tgggggcact
    68461 ggcgaaacgg atgccgtgga cagccgcagc atgcctgatt ggttgcctcg cgatatcagc
    68521 cattcctccg ctgaatggtt ttatcagcga atggtacacc tggcagtcgc tgttctcact
    68581 aagtcgtgtg gaagccgtag cgctacaact tgcgggtcct attgctatgg taatgctggc
    68641 agtcactggt gggctggcag taatgtgctt cgtcaaaatg tacggtatta ctttctgtgg
    68701 tgcgccgcgc agtacacacg ctgaagaggc acaggaagtg ccaaatacga tgatcgtcgc
    68761 catgctactg ctcgcggcac tctgcgtatt aattgcgctt agtgccagtt ggctggcacc
    68821 gaagataatg catattgccc atgcgtttac caatacccct cccgtcactg tcgccagcgg
    68881 aatagcactt gtacccggca cgtttcatac acgggtcact ccttcattac tgttgctgtt
    68941 actactggcg atgcctttgc tgccaggcct ttactggctg tggtgtcgtt cgcgccgcgc
    69001 agcgtttcgt cgcacaggag atgcctgggc atgcggctac ggctgggaaa acgcgatggc
    69061 tccgtcaggc aatggcgtga tgcagccgct gcgtgtggtc ttctctgcgt tatttcgtct
    69121 acgacaacag ctcgacccta cgctgaggct aaataaaggt cttgcgcacg tcaccgccag
    69181 ggctcagagc acagaaccct tctgggatga gcgggtgatc cgccccatcg tgagcgccac
    69241 ccaacggctg gtcaaagaaa tacagcatct gcaaagcggc gactttcgtc tctattgcct
    69301 gtatgtggtc gccgcactgg ttgtgctgct aatcgctatt gccgtctaag gaaatcacca
    69361 tgagacaaac tctttgcgac ggatatctgg tcatttttgc gttagcacag gccgtgattc
    69421 tgctgatgct aaccccactt tttacgggta tttcccggca gatacgcgcg cgtatgcact
    69481 cccgccgcgg gccggggatc tggcaggatt atcgcgatat ccacaaactg tttaaacgcc
    69541 aggaagttgc gccgacatct tcaggtctga tgttccgcct gatgccgtgg gtattaatca
    69601 gcagcatgct ggtgctggcg atggccttac cactatttat taccgtttcc ccttttgcgg
    69661 gcggcggcga tctgatcacc cttatctatc ttcttgccct gtttcgtttt ttctttgctc
    69721 tttccgggct ggataccgga agtccgtttg cgggagtcgg tgccagtcgc gagttgacgc
    69781 tcggcattct ggtcgaacca atgcttattc tctcactgct ggtattggcg ctgatagcag
    69841 gttccacgca tatcgagatg atcagcaata cgctggctat gggctggaac tcgccgctaa
    69901 ccaccgtact ggcgttactg gcctgtggtt ttgcctgctt cattgagatg ggaaaaattc
    69961 cctttgatgt tgctgaagca gaacaggaat tacaggaagg cccgctgacc gaatattccg
    70021 gtgccgggct ggcgctagcg aaatgggggc tggggctgaa acaggtcgtg atggcagcac
    70081 tgtttgtggc cctgtttctg ccctttgggc gcgcgcaaga actttctctc gcctgcctgc
    70141 tgacttcact tgtcgttacg ctgctcaagg ttttgctgat ttttgtactg gcctcaatcg
    70201 cagaaaacac gctggcacgc gggcgttttt tactcattca ccatgtgacc tggcttggct
    70261 tcagccttgc tgcgcttgca tgggtctttt ggttaaccgg tctgtaagga gcactgacgg
    70321 aatatggaaa atcttgctct gacgacgtta atgctgcctt ttatcggcgc actggtcgtt
    70381 tcgttttcgc cacaacgtcg ggccgccgaa tggggggttt tgttcgccgc gctgaccacg
    70441 ctgtgcatgt tgtcgctgat ctccgcgttt tatcaggccg ataaagttgc cgtcacgttg
    70501 acgttggtca acgtggggga tgtggcatta tttggcctgg tcattgatcg cgtgagtacg
    70561 ctgattctgt ttgtggtggt gtttctcggt ttgctggtca cgatctactc cacgggttat
    70621 ctgacggata aaaatcgcga acacccgcat aacggcacga atcgttatta cgcatttttg
    70681 ctggtgttta tcggcgcgat ggcgggactg gtactctcct cgacgctgct cggtcagttg
    70741 ttgttttttg aaattacggg cggctgctcc tgggcgttga tcagttatta ccagagcgat
    70801 aaagcgcagc gttcagcact aaaagcgttg cttatcaccc atatcggttc gttggggttg
    70861 tatcttgccg ccgccacgct gtttttgcag accggaacgt ttgtgcttag cgcgatgagc
    70921 gagttacatg gcgacgcacg ttatctggtt tatggcggca tcctgtttgc cgcgtggggg
    70981 aaatcggccc agctaccgat gcaagcgtgg ctaccggacg caatggaagc gccaacaccg
    71041 atcagcgcct atctccacgc cgcatcgatg gtgaaagtgg gcgtttacat ttttgcccgc
    71101 gccattatcg acggcggcaa tatcccgcat gtgattggcg gcgttggcat ggtcatgaca
    71161 ctggtcacca ttctttatgg ctttctgatg tatttgccac agcaggatat gaagcggttg
    71221 ctggcctggt cgaccatcac tcaacttggc tggatgttct tcggcttgtc gctctccatc
    71281 ttcggctcgc ggctggcgct ggagggtagc atcgcctaca tcgtcaacca cgcgttcgct
    71341 aaaagcctgt ttttccttgt agcaggtgcg ctgagttaca gctgcggcac gcgcttgttg
    71401 ccgcgtctgc gtggcgtatt gcacaccctg ccgttgccag gcgtgggttt ctgcgtggca
    71461 gcgctggcaa ttaccggtgt gccgccgttc aacggcttct tcagtaaatt cccgctgttt
    71521 gccgccggtt ttgcgttgtc agtggagtac tggatcctgc tgcccgccat gattctgctg
    71581 atgattgaat cggtcgccag tttcgcctgg tttattcgct ggtttggtcg cgtcgtgccg
    71641 ggcaaaccga gcgaggccgt cgccgatgcc gcaccgctgc cagggtcaat gcgcctggtg
    71701 ttgattgtac tgattgtgat gtcgctgatt tccagcgtaa tcgccgcgac ctggttgcag
    71761 taaggagatg atgaatgacc ggttctatga tcgtaaataa tctggcggga ctgatgatgc
    71821 tgacatcgct gtttgtgatt agcgtcaaaa gctatcgcct gtcatgcgga ttttacgcct
    71881 gccagtcgct ggtgctggtg tctattttcg ccactctctc gtgcctgttc gccacagagc
    71941 aactgctgat ctggtccgcc agcgccttta tcaccaaagt gctgctggta ccgttaatca
    72001 tgacttacgc tgcacgaaat attccccaga acatcccgga aaaagcgtta ttcggtccgg
    72061 caatgatggc actgctcgcg gcgttaattg tcctgctttg cgcatttgtc gttcagcccg
    72121 tgaagctacc gatggctacc gggctgaaac cggcgctggc ggtagcgtta ggtcattttc
    72181 tgcttggcct gctgtgcatt gtcagccaac gcaatatcct gcggcaaatt tttggttact
    72241 gcctgatgga aaacggctcc catctggtgc tggcgcttct tgcctggcga gcaccggaac
    72301 tggtggaaat aggtatcgct accgacgcca tcttcgccgt cattgtgatg gtgttactgg
    72361 caagaaaaat atggcgtacc cacggcacgc tggacgtgaa caacttgacc gcgctgaagg
    72421 gataatgaga tgagttattc tgtgatgttc gctttactcc tgctcacgcc gctgcttttt
    72481 tcgctgctct gttttgcctg ccggaaacgg gggctctctg cgacttgtac ggtgactgta
    72541 ttacatagct tagggatcac actgctgctg attctagcac tctgggtggt ccaaactgcc
    72601 gctgatgcag gagaaatatt cgctgcggga ctgtggcttc atattgatgg tctgggcggt
    72661 ttgttcctcg ccattcttgg tgtgattggc tttctcaccg gtgtttactc gattggctac
    72721 atgcgtcatg aagtggcaca cggcgagctt tcacccgtta cactgtgcga ttactacggt
    72781 ttcttccatc tgtttttgtt caccatgctg ctggttgtta ccagcaataa cctgattgtg
    72841 atgtgggcgg cgatcgaagc caccacctta agctcggcgt ttctggtagg catttacggt
    72901 cagcgttcat cgctggaatc agcatggaag tacatcatta tttgtactgt tggtgtcgct
    72961 tttggtctgt tcggtaccgt gctggtatac gccaacgccg ccagcgttat gccgcaggca
    73021 gaaatggcga tattctggag cgaggttctt aagcaatcgt ccttgcttga cccaacatta
    73081 atgctgttgg cctttgtgtt tgtgctaatt ggctttggta ccaaaaccgg gctatttccc
    73141 atgcacgcct ggctgccgga tgctcacagt gaagcgccga gtccggtcag cgccctgctc
    73201 tccgccgtat tgctgaactg cgcgctgttg gtgctgattc gctattacat cattatttgc
    73261 caggccatcg gcagcgattt ccccaaccgg ttgttgctca tcttcggcat gttgtcggtt
    73321 gccgtggcgg catttttcat tctggtacag cgggacatta agcgtctgct ggcgtactcc
    73381 agcgtggaga acatggggct ggtcgcggtg gcgctaggca ttggcgggcc gctgggaatt
    73441 tttgccgcgc tgctgcacac cttaaaccac agtctggcaa aaacgctgct gttctgcggt
    73501 tccggcaatg tactgctcaa gtacggcacg cgcgatctca acgtcgtctg tgggatgctc
    73561 aaaatcatgc catttaccgc cgtgctgttt ggcggcggtg cgctggcgct ggcgctggcg
    73621 ctggcaggga tgccgccctt caacattttt cttagcgaat ttatgaccgt taccgcagga
    73681 ctggcacgta atcacctgct gattatcgtc ctgctgttat tgctgttaac gctggtgctg
    73741 gcgggcctgg tacggatggc tgcgcgggtg ttaatggcga aaccgccgca ggccgttaac
    73801 cggggtgatc tcggctggtt gaccacctcg ccaatggtga ttctgctggt catgatgctg
    73861 gcgatgggaa cgcatattcc acaacctgtc atcaggatcc tggcgggcgc ttccactata
    73921 gtcctctcag ggacgcacga cctgcctgca caacgtagca cctggcatga ttttttgcct
    73981 tcaggcaccg catctgtttc ggagaaacac agtgaacgtt aattcatcgt caaatcgtgg
    74041 cgaagcgatt ctcgccgccc tgaaaacgca gttccccggc gcggtgctgg atgaagagcg
    74101 acaaacgcct gaacaggtca ccattacggt gaaaatcaat ctgctgcctg acgttgtaca
    74161 ttatctttat tatcaacatg atggctggct tccagtcctg tttggcaacg acgagcggac
    74221 acttaacggt cattacgcgg tttattatgc cctttcaatg gaaggggccg aaaaatgctg
    74281 gatcgtggtg aaggcactgg tcgatgccga cagtcgggag tttccgtcag tcacaccgcg
    74341 cgtccctgcc gcggtctggg gcgagcgaga aattcgcgat atgtacgggc tgattccggt
    74401 tggcctgccg gatcagcgtc gcctggtgtt gcccgatgac tggccggaag atatgcatcc
    74461 gctgcgcaaa gatgcgatgg attatcgact gcgccctgaa ccgacgacag attccgaaac
    74521 gtatgcgttt atcaatgagg gcaacagcga tgcgcgggtg atccctgtcg gcccgctgca
    74581 tatcacctcc tatgaaccgg gtcacttccg cttgtttgtg gatggcgagc aaattgtcga
    74641 tgctgattac cgcctgtttt atgtccatcg cggcatggag aaactggcag aaacgcggat
    74701 gggctacaac gaagtgacct tcttatcgga ccgcgtgtgt gggatttgcg gttttgccca
    74761 cagtgtggcc tataccaact cggttgaaaa tgcactgggg attgaggtgc cgcaacgagc
    74821 acatactatt cgctcgattc tgctggaagt cgagcggcta cacagtcatt tgctcaacct
    74881 tggcctctcc tgccattttg ttggttttga taccggcttt atgcaatttt tccgcgtgcg
    74941 ggaaaagtcg atgacgatgg cggaattgct gaccgggtcg cgtaaaacct acggtctgaa
    75001 tctgattggt ggtgttcgcc gcgatattct caaagagcaa cgtctgcaaa cgctgaaact
    75061 ggtgcgcgag atgcgcgccg acgtgtcgga gctggtagag atgctgcttg ctacgccgaa
    75121 tatggaacaa cgcactcagg gcattggcat tctcgaccga caaatcgccc gtgattatag
    75181 ccctgtaggg ccgctgatcc gcgcagtggt tttgcccgtg atttgcgctt tgatcacccc
    75241 tacgccgact acggcaatat tccaaaaaca ctgtttacct ttaccggcgg cgatgttttc
    75301 tcccgcgtga tggtccgtgt caaagagacg tttgattcgc tggcaatgct ggaatttgcc
    75361 ctcgacaaca tgccggatac cccactgctg accgaaggct ttagctataa acctcacgca
    75421 ttcgcgctgg gctttgttga agcgccacgc ggtgaagacg tgcactggag catgctcggt
    75481 gataaccaaa aattgttccg ctggcgctgt cgtgccgcca cctacgccaa ctggccggtg
    75541 ttgcgttaca tgctgcgcgg caataccgtt tctgacgcac cgctgattat cggtagcctt
    75601 gatccctgct actcctgtac cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca
    75661 aaaaccgtgc tgtataaaga gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg
    75721 aagtaaggac agaagatgct gaagttactg aaaactatta tgcgcgccgg aaccgcgacg
    75781 gtgaaatatc ccttcgcgcc actggaggtc agcccaggct ttcgcgggaa accggacctg
    75841 atgcccagcc aatgtattgc ctgcggtgcc tgcgcctgtg cttgtccggc aaatgcgctg
    75901 actatccaga ccgacgacca gcaaaattcg cgcacctggc agctctatct ggggcgttgt
    75961 atttactgcg gacgttgtga agaagtgtgc ccgaccagag ccatccagct taccaataac
    76021 tttgaactga ccgtcaccca taaagccgat ctctataccc acgcgacgtt ccatctacaa
    76081 cgttgcagcc gttgcgaacg cccgtttgcc ccgcaaaaaa ccgtcgcact ggctgctgaa
    76141 ttgttggcac agcaacaaaa tgctccacaa aaccgcgaaa tgttatgggc gcaagcgagc
    76201 gtctgcccgg agtgcaaaca acgcgcaacg ctgatcaacg acgatacaga tgtaccgctg
    76261 gtggctaagg agcagctatg agtccagtgc ttacgcaata tgtcagccag cccatcacgc
    76321 tggacgagca aacgcaaaag atgaagcggc atttgctaca ggatatccgt cgctcggctt
    76381 acgtttatcg catcgattgc ggcggctgca acgcctgtga aatcgaaatt tttgctgcca
    76441 tcacccccgt attcgacgca gaacgttttg gcattaaggt tgtttcatca ccgcgtcacg
    76501 ccgatatttt gttatttact ggcgcagtca cccgggcgat gcgtatgcct gcacttcggg
    76561 cgtatgagtc tgcccccgat cataaaatct gtgtttccta cggcgcgtgc ggtgtcggcg
    76621 gcggtatttt ccacgatctc tacagcgtct ggggcggcag cgacaccatt gtccccattg
    76681 atgtttggat ccccggctgc ccgccaacac cggccgccac cattcacggt ttcgccgtgg
    76741 cgctcggttt gctgcaacag aagattcacg cggtggatta tcgcgatccc accggggtga
    76801 ctatgcaacc gttgtggccg cagatcccgc catcacagcg tatcgccatt gagcgagaag
    76861 cgcggcggct ggcgggctat cgtcaggggc gagaaatttg cgatcggctc ctgcgccatt
    76921 taagcgacga tcctacagga aatcgggtta acacctggtt gcgcgatgcc gacgatccac
    76981 gtctcaatag tatcgttcag caactctttc gcgtactccg ggggttacat gactgaagag
    77041 tgcggggaaa ttgttttctg gacgctgcga aaaaagtttg tcgccagtag cgacgagatg
    77101 ccggaacaca gctctcaggt aatgtattac tcgctggcta tcggtcatca cgttggcgtg
    77161 attgattgtc tgaatgtcgc cttccgctgc ccactgacgg aatacgaaga ttggcttgca
    77221 ctggtcgaag aggagcaagc ccgacgtaag atgctggggg tgatgacttt tggtgagatt
    77281 gttattgacg ccagccacac cgccctgttg acccgggcat tcgcgccact ggcagatgac
    77341 gcgacgtctg tgtggcaagc gcgtagcatt caattcattc atctgttgga tgaaattgtg
    77401 ctggaaccgg ctatctatct gatggccaga aaaattgcgt gagaaggatt tctcattaat
    77461 aaggactgtt gatggctatg tcagacgaag cgatgtttgc cccgccgcaa ggaataacaa
    77521 ttgaagcggt aaacggaatg ctcgcggagc ggttagcgca gaaacacggc aaggcgtctt
    77581 tattacgcgc cttcatcccg ctgccgccgc cgttcagccc ggtacaactt attgaactgc
    77641 atgttctcaa aagcaacttc tattaccgct accatgatga tggcagcgat gtgacggcaa
    77701 caacagagta tcagggcgag atggtcgatt attcgcgtta cgccgtcctt ctcggcagta
    77761 gtggaatggc ggagctacgc tttattcgta cccacggcag ccgttttact ccccaggatt
    77821 gcacactgtt taactggctg gcacgcatta tcaccccggt tctgcaatca tggctcaatg
    77881 atgaagcaca gcaggtggcg ctgcgtttgc tggagaaaga tcgcgatcat catcgggtac
    77941 tggtggatat cactaatgca gtgctgtcac atcttgatct cgacgatctg atcgctgacg
    78001 tcgctcgtga gatccatcat tttttcggtc tggcttcagt cagtatggta ctgggcgatc
    78061 atcgaaagaa cgagaagttc agcctgtggt gcagcgatct ttctgcctca cattgtgcgt
    78121 gtctgccaca caatatgcct ggcgacagtg tattgctgac acaaacgcta caaacccgac
    78181 aaccgacctt gacgcaccgt gcagatgatc tgtttctctg gcaacgcgac ccgttattac
    78241 tcttacttgc atctaacggc tgcgaatctg cgctccttat accgcttacc tttggcaacc
    78301 atacaccggg tgcattgttg ctggcgcata cctcttccac tctctttagt gaggaaaact
    78361 gccagctact acaacacata gccgatcgca tcgctattgc cgttggcaat gccgatgcct
    78421 gccgtagaat gaccgatttg caggaaagtt tgcagcaaga aaaccaccag cttagcgagc
    78481 agctcctttc gaatctgggc gtcggtgaca ttatctatca aagccaggca atggaagacc
    78541 tgctccagca ggtagatatt gtggcgaaga gcgacagtac ggtgttgatt tgcggtgaaa
    78601 ccggaaccgg caaagaggtg atcgccagag cgatccatca acttagcccg cgacacgaca
    78661 agccgctggt caaaatcaac tgcgctgcca tccccgccag tcttctggaa agtgagttat
    78721 tcggtcatga caaaggggcg tttactggtg cgattaatac ccatcgtggt cgttttgaaa
    78781 ttgccgatgg cggcacgttg tttctcgatg aaattggcga tctgccgtta gaacttcagc
    78841 ctaaactgct gcgcgtattg caggaacggg agattgagcg tctcggcggg agtagaacga
    78901 tcccggtaaa tgtcagagtc attgccgcca ccaaccgtga tttgtggcaa atggttgaag
    78961 atcgccagtt tcgcagcgat ctcttttatc gcctgaatgt cttcccactg gaattgcctc
    79021 cgctgcgcga ccgtccggaa gatatccctc ttttagcaaa gcatttcacg caaaaaatgg
    79081 cgcgccatat gaatcgttca attgacgcca tcccgaccga ggcgctacgc cagttgatgt
    79141 cgtgggactg gccgggcaac gtgcgcgagc tggaaaacgt gattgagcga gcggtactgc
    79201 tgactcgcgg taacagtctg aatttacatc ttaatgtccg acaaagccgt ttactgccga
    79261 cgctaaatga agattcagcg cttcgcagtt caatggcgta gttgctgcac ccgacgacgc
    79321 cagagaatga cgaagaagaa cgtcagcgca ttgttcaggt attgcgagaa accaatggca
    79381 ttgttgccgg gccccgtggc gcggcgacac gattagggat gaagcgcacc acgctgctgt
    79441 cacgaatgca gcgtctggga atctcggttc gcgaggtgtt gtaatctgct tttgcaggag
    79501 tatgcatgag aaacaaactc tctttcgact tgcagttgag cgccagaaaa gcggcaatcg
    79561 ctgaacggat tgccgcccat aaaattgccc gcagtaaagt gtcggtcttt ttaatggcga
    79621 tgtccgctgg cgtgtttatg gcgatgtccg ctggcgtgtt tatggcgatc ggatttactt
    79681 tttacctttc cgttatcgcc gatgcccgtc ttcataggca ttaacccatc tggtgggcgg
    79741 cctttgcttt acacttggct ttattttgct ggcggtttgc ggcaccagcc tgttcacctc
    79801 gtcggtaatg acggtaatgg caaaaagtcg gggcgttatt agttggcgaa cttggctgat
    79861 taacgcactt ctggtggcct gcggtaatct ggcaggtatt gcctgtttca gtttgttaat
    79921 ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg ggagtcgcga ttttacactg
    79981 cgccgagggc aaaatgcatc atacatttac tgaatctgtc agcctcggca ttatgtgcaa
    80041 tctgatggtt tgcctggcgc tgtggatgag ttattgcgga cgttcgttat gcgacaaaat
    80101 cgtcgccatg attttgtcca tcaccctgtt tgtcgccagt ggctttgagc actgtatcgc
    80161 caatttgttt gtgattccgt tcgccattgc cattcgccat ttcgccccta cctccttctg
    80221 gcaactggcg cacagtagcg cagacaattt tccggcactg acggtcagcc attttattac
    80281 cgccaatctg ctcccggtga tgctgggtaa tattatcggc ggtgcagtgc tggtgagtat
    80341 ttgttatcgg gctatttatt tacgtcagga atcctgaaaa atcagcccgg cgaaacagtt
    80401 cgtcgggcta tttatttacg tcaggaatcc tgaaaaatca gcccggcgaa acagttcgtc
    80461 gggctgaaga ttattcttgc gcgatttgcc catcgggcca ggcgtggatc acggctttga
    80521 tcagcgtcgc caatggaatg gcgaagaata cgccccagaa tccccacaaa ccaccgaaga
    80581 tcaccaccga taaaataatc accagcggat gcaggttaac cgcttcggag aacaacaccg
    80641 gtactagcag gttgccgtcc agcgcctgaa taatcagata caccgcgaag cagctccaga
    80701 attcagtacc tgcgccaaac tggaatagcg ccacgccaac caccggaatg gtcaccacaa
    80761 atgcgccaat gtacggaata agaaccgaga agccgaccag cactgccagc agcagcgaat
    80821 agttcagccc gaagagcaag aaccccagcc aggtggcgat ccccaccacg atcatctcca
    80881 gcactttgcc gcggatatag ttggtgattt gttgattcat ctccttccac acctgtcctg
    80941 ccagcccacg gttacgcggc agcacccggc gaacggcgtt cagcatctgc tctttgtctt
    81001 tcagcaggaa gaagaccatc aatggcacca gcaccagata gacggctatg gtcagcaaac
    81061 cgaccagcga ggcgagggaa attttcacca ccgaatcgcc catggtcagc atccgactgc
    81121 gcatattttc ggccattgca tcaataatgc ccgcgtccat taacaccgga tagcggcgcg
    81181 gcaacgtggc ggcaaagtca gaaagcttat tgagcatccc cggcatatcg cggattaagt
    81241 agatgccctg ttgccaggcg ataggcagta ccacgaacgc catcagtagc aatataccga
    81301 caaaaaccac caatacaatc gacgtcgccc agcggcggga gcagccaata gattgcaggc
    81361 gcacggttgg ccattccagc aaataagcca gcacaatagc caccagcaac ggagcaagca
    81421 ggccactaaa gaaaaagata atgccaaatc cggcaactaa aataaccagc aaggcaatcg
    81481 cttccgggtc gctaaaacgg cggcgatacc attgcatcaa catttcgagc ataaagacct
    81541 tccctgaacc tcaagagcgg gattgcgatc cgcaattgta tcgaaatgtc acaaaaaaga
    81601 cttcgctttt tatgacggat tcaggaaact gaaaagtcat ttgagtgggc taatcttcgc
    81661 cgttacactc aaaggcggcg aggtgggaac gatatttcac agtatcggtc aaatgactat
    81721 cttcagaaat acaggataga ggttatgttc aggcagttga aaaaaaacct ggttgcaacc
    81781 ctcattgctg ctatgaccat tggtcaggta gccccggcgt ttgccgacag cgcagacacc
    81841 ttgccggata tgggaacctc cgcaggaagc acgctttcca ttggtcagga aatgcagatg
    81901 ggcgactatt atgtccgcca gctacgcggc agcgcgccgt taattaatga cccgctgtta
    81961 acgcaatata ttaattcgct ggggatgcgt ctggtttcgc atgccaattc ggttaagaca
    82021 ccgtttcatt tttttctgat caacaacgac gaaattaacg cctttgcttt ctttggcggc
    82081 aacgtggtgc tgcactctgc cctgttccgt tattccgata acgaaagtca actggcttca
    82141 gttatggcgc acgaaatctc ccacgtcacc caacgtcacc tggcgcgagc gatggaagat
    82201 caacaacgca gcgcgccgct gacctgggtc ggcgcgttag gttctatttt actggcgatg
    82261 gccagtccgc aggcggggat ggcggcgctg accggtacac tggcgggaac gcgccagggg
    82321 atgatcagtt tcacccagca aaatgaacag gaagcggacc gcattggtat tcaggtgctg
    82381 caacgctcgg gattcgatcc acaggcgatg ccgaccttcc tggaaaaatt gctcgatcag
    82441 gcgcgttact cctcgcgccc gccggaaatt ttattgactc acccgttgcc ggaaagtcgt
    82501 ctggcagatg cccgcaaccg tgctaatcag atgcgcccga tggtggtgca gtcgtcggaa
    82561 gatttctatc tggcgaaagc gcgcacactg gggatgtata attccggacg taaccagctc
    82621 accagtgatt tgctggatga atgggcgaaa ggaaacgttc gtcagcaacg agcggcgcaa
    82681 tatggtcgtg ctttacaggc gatggaagcc aataaatacg acgaggcgcg taaaacgctg
    82741 caaccgttac tggcggcaga acctggcaac gcatggtatc tcgatctggc taccgatatc
    82801 gatcttgggc aaaacaaagc caatgaggca atcaatcgcc tgaaaaatgc ccgcgattta
    82861 cgcaccaatc cggtgttgca gctcaacctg gcgaacgctt atctgcaagg cggtcaacca
    82921 caagaagcgg ccaatattct gaatcgctac acctttaata ataaagatga cagcaacggc
    82981 tgggatttgc tggcacaggc ggaagccgcg ctaaataacc gcgatcagga gctggctgcg
    83041 cgagcagaag gttatgcgct cgccggacga ctcgatcagg ccatttcgct gttgagtagc
    83101 gccagttcgc aggtgaaatt aggcagcctg caacaagcgc gttacgatgc gcgcatcgac
    83161 cagttgcgcc agctgcagaa acgctttaag ccttatacca agatgtaatt cgcattaaag
    83221 gaagaacaat gaccaaacag gtaaaaatct accataaccc acgctgttca aagagccggg
    83281 aaacgctgaa cctgctgaaa gaaaacggcg tggagccaga agtggtgctc taccttgaga
    83341 cacccgccga tgcggcaacg ctgcgcgatt tgctgaaaat gctggggatg aatagcgccc
    83401 gtgaattgat gcgccagaaa gaggatcttt ataaagaact aaacctggct gacagttcgc
    83461 ttagcgaaga ggcgctgatt caggcaatgg tggataatcc gaagctgatg gagcgcccga
    83521 ttatggtcgc gaatggcaaa gcgcggattg gtcgtccacc ggagcaggta ctggaaatcg
    83581 tgggttaaga tgtgctccat cgctagttga agcacattgc cggatgcgac gcttgccacg
    83641 tcttatccgg cctacgaata ctatggatta acttcgcgta ggtcaggcgt tcgcgccgca
    83701 tccgacaata aacaccttat ctacaacttc agaatttctt tcacaaacgg aatggtcagc
    83761 ttacgttgcg cggtaatcga cgcacgatcc aactgatcca acgtcataaa tagcgtgcgc
    83821 atttctctgt cgagccgctt cagcaagaaa cgccccacat cttccggcag ttcaaaacca
    83881 cgcaaacgcg cgcgtaactg tagcgcctgc aacttatctt catcagaaag tggctgcaat
    83941 ttgtagatct gcccccagtc gagacgcgac gcgagatccg gtaatcccag attcaactgc
    84001 cgcggtggac gatcgccggt gatcaacaac cgtgttttgc ccgattccag aattcgattg
    84061 tagagatcga aaatcgccat ctcccacaac tcatcgcctg caatacactc aatgttgtcg
    84121 atacagacca gcgacaaatg ctccataccg tcgagcactt ccggaacaaa ccaggtgcgt
    84181 ttatccagcg ggacatagcc caccgcatcg ccacgctgcg acaattccgc gcaagccgcg
    84241 tgcagcagat ggctgcgccc cgcgccttcg cgtgcccaga gatagatgta accgctatgt
    84301 tcctgacgca gcacgttttg cagcgcggcc agtaaagagg agttatcccc cggccagaaa
    84361 cttgcaaagg tttcgtcgtc aggaagataa agtggcaaag agagctgtgc cggtgtgttc
    84421 agagatacct caaccaggat ttcacaaaat cgcgagaagt ttaccacaga atcccatgat
    84481 gtttgaaccg ggcagcaaca ctgcccggtc ggtacattat ttgtctgtta tatccgcgtc
    84541 ttctgcgtcc agcaccactt cttcagggcg cagcacactg atcagtttga aaatcaaact
    84601 taagccgata ccgacgatgg tcgccagcgc catacctttt aactccgccg cgccgatgtt
    84661 taccttcgcg ccactgacgc cgatgatcaa aatcacggaa gtcaggatca ggttctgtgc
    84721 tttgttgtaa tccactttcg attcgatcaa aacacgaata ccggaagcac caatgacacc
    84781 ataaagcagc agcgaaacgc cgcccatcac cggcaatggg atcatctgga tagcggcagc
    84841 cagtttaccg acgcaggaaa gcaggatagc gaaaatcgcc gccccgccga taacccaggt
    84901 actgtaaaca cgggtgatcg ccatcacgcc aatgttttct ccgtaagtgg tatttggcgt
    84961 agagccaaag aagccggaga tcacggtcga caggccgtta gcaaacatcg aacggtgcag
    85021 acctggatcg cgcagcagat cttttttgac gatattagcc gttactacca ggtgccctac
    85081 gtgttcggca ataaccacta acgccgctgg cagaatagtc agaatggcaa accactcgaa
    85141 gcgcggcgta tagagggttg gcagtgcaaa ccagtgagca ttaataatcg gcgtggtatc
    85201 gacaattccc attgcgaaag agagcgcgta ccccaccagc acgccaatta aaatcgggat
    85261 aattgccagg aaaccacgaa acagcacgga acctaaaacc gtgaccgcca gggtggtaat
    85321 agagatgatg atggttttgg agtttggcgt ttgcccttca gccgggagta aacccgccat
    85381 accggcagct acgcccgcca gctccagacc gatgacggca acgattgcgc ccattgccgc
    85441 aggtggaaac agcacgtcca accagccggt ccccgctttc ttcacgataa aagaaaccag
    85501 gcagaacagc acgccgcaca taataaagcc gcccagcgcg acttcatacc ctaacggcaa
    85561 cagtaacaat accggtgaaa taaaggcaaa gctggaacca agataagccg gaattttccc
    85621 tttacagatg aagagataca gcagcgttcc aataccgtta aataacagta cagtcgccgg
    85681 gttaatatga aataagacgg gcaccaggac ggttgcacca aacatggcga acaaatgttg
    85741 caaactaagc gggattgtct gtaaaagtgg cggtctttca ctcaccccga tagcacggcg
    85801 cgtcatagta ttatcctctg tattatgtgt tataggcgct ttactcaaaa aaaagccgac
    85861 tcttaaagtc ggctttaatt atttttattc tttatttcgt accaaagatt ttgtcaccgg
    85921 catcgccgag gcccggaata atgtatccgt gctcgttcag tccctggtca atcgatgcag
    85981 tatacagttc gacgtccggg tgcgcttttt ccagcgcagc gataccttct ggcgcagcga
    86041 ccagcaccag aactttgatg ctgctgcagc ccgctttttt cagcaggtcg atggtcgcga
    86101 taacggaacc accggttgcc agcattgggt caacgatcag cgccatacgc tcatcgatgt
    86161 tagaaaccag tttctggaag tacggtaccg gctccagcgt ttcttcatta cggtacatac
    86221 cgacaacgct gatgcgcgcg ctcggaacgt tttccagcac accgtccatc ataccaagac
    86281 ccgcacgcag aattggcaca acggtaattt tcttaccttt gatctggtcg atttctaccg
    86341 ggccgttcca gccttcgata gttacttttt ccgtttcgag gtcggcggtc gcttcgtaag
    86401 tcagcaggct acccacttcg gaagcgagtt cgcgaaagcg cttggtgctg atatcttgct
    86461 cacgcatcag tcccagcttg tgtttgacga gtgggtgttt gacttccacg atcttcatac
    86521 tctttctcct ttgaggggca gccacaaaaa aatcgacgga ttatacctcc tttcttcagg
    86581 gcggcaatat tattttcgtt gactttagtc aaaatgataa cagtttgaga taaagttatt
    86641 ttatattcaa atggttatga aagaagatta ttccatccga aaactaacct ttgccctggc
    86701 acaagtcttc tttcgccgcg cgcccgggga aaagacgtgc aaaaaggttg tgtaaaacag
    86761 tctcgcaaac gtttgctttc cctgttagaa ttgcgccgaa ttttattttt ctaccgcaag
    86821 taacgcgtgg ggacccaagc agtgaccgat aaaacctctc ttagctacaa agatgccggt
    86881 gttgatattg acgcgggtaa tgctctggtt ggaagaatca aaggcgtagt gaagaaaacg
    86941 cgtcgtccgg aagtgatggg cggtctgggc ggcttcggtg cgctgtgtgc attgccgcaa
    87001 aaatatcgtg aacccgtgct ggtttccggc actgacggcg taggtaccaa gctgcgtctg
    87061 gcaatggact taaaacgtca cgacaccatt ggtattgatc tggtcgccat gtgcgttaat
    87121 gacctggtgg tgcaaggtgc ggaaccgctg tttttcctcg actattacgc aaccggaaaa
    87181 ctggatgttg ataccgcttc agcggtgatc agcggcatcg cggaaggttg tctgcaatcg
    87241 ggctgttctc tggtgggtgg cgaaacggca gaaatgccgg ggatgtatca cggtgaagat
    87301 tacgatgtcg cgggtttctg cgtgggcgtg gtagaaaaat cagaaatcat cgacggctct
    87361 aaagtcagcg acggcgatgt gctgattgca ctcggttcca gcggtccgca ctcgaacggc
    87421 tattcgctgg tgcgcaaaat tcttgaagtc agcggttgtg atccgcaaac caccgaactt
    87481 gatggtaagc cattagccga tcatctgctg gcaccgaccc gcatttacgt gaagtcagtg
    87541 ctggagttga ttgaaaaggt cgatgtgcat gccattgcgc acctgaccgg cggcggcttc
    87601 tgggaaaaca ttccgcgcgt attgccagat aatacccagg cagtgattga tgaatcttcc
    87661 tggcagtggc cggaagtgtt caactggctg caaacggcag gtaacgttga gcgccatgaa
    87721 atgtatcgca ccttcaactg cggcgtcggg atgattatcg ccctgcctgc tccggaagtg
    87781 gacaaagccc tcgccctgct aaatgccaac ggtgaaaacg cgtggaaaat cggtatcatc
    87841 aaagcctctg attccgaaca acgcgtggtt atcgaataat gaatattgtg gtgcttattt
    87901 ccggcaacgg aagtaattta caggcaatta ttgacgcctg taaaaccaac aaaattaaag
    87961 gcaccgtacg ggcagttttc agcaataagg ccgacgcgtt cggccttgaa cgcgcccgcc
    88021 aggcgggtat tgcaacgcat acgctcatcg ccagcgcgtt tgacagtcgt gaagcctatg
    88081 accgggagtt gattcatgaa atcgacatgt acgcacccga tgtggtcgtg ctggctggtt
    88141 ttatgcgcat tctcagcccg gcgtttgtct cccactatgc cgggcgtttg ctgaacattc
    88201 acccttctct gctgccgaaa tatcccggat tacacaccca tcgtcaggcg ctggaaaatg
    88261 gcgatgaaga gcacggtaca tcggtgcatt tcgtcaccga tgaactggac ggtggcccgg
    88321 ttattttaca ggcgaaagtc ccggtatttg ctggtgatac ggaagatgac atcaccgccc
    88381 gcgtgcaaac ccaggaacac gctatttatc cactggtgat tagctggttt gccgatggtc
    88441 gtctgaaaat gcacgaaaac gccgcgtggc tggatggtca acgtctgccg ccgcagggct
    88501 acgctgccga cgagtaatgc ccccgtagtt aaagcgccag ctctgccgct ggcgtttttc
    88561 aattcacctg taaatcgcaa gctccagcag tttttttccc ccttttctgg catagttgga
    88621 catctgccaa tattgctcgc cataatatcc aggcagtgtc ccgtgaataa aacggagtaa
    88681 aagtggtaat gggtcaggaa aagctataca tcgaaaaaga gctcagttgg ttatcgttca
    88741 atgaacgcgt gcttcaggaa gcggcggaca aatctaaccc gctgattgaa aggatgcgtt
    88801 tcctggggat ctattccaat aaccttgatg agttctataa agtccgcttc gctgaactga
    88861 agcgacgcat cattattagc gaagaacaag gctccaacgc tcattcccgc catttactgg
    88921 gcaaaattca gtcccgggtg ctgaaagccg atcaggaatt cgacggcctc tacaacgagc
    88981 tattgctgga gatggcgcgc aaccagatct tcctgattaa tgaacgccag ctctccgtca
    89041 atcaacaaaa ctggctgcgt cattatttta agcagtatct gcgtcagcac attacgccga
    89101 ttttaatcaa tcctgacact gacttagtgc agttcctgaa agatgattac acctatctgg
    89161 cggtggaaat tatccgtagc gatagcatcc gttacgcgct gctggagatc ccatcagata
    89221 aagtgccgcg ctttgtgaat ttaccgccag aagcgccgcg tcgacgcaag ccgatgattc
    89281 ttctggataa cattctgcgt tactgccttg atgatatttt caaaggcttc tttgattatg
    89341 acgcgctgaa tgcctattca atgaagatga cccgcgatgc cgaatacgat ttagtgcatg
    89401 agatggaagc cagcctgatg gagttgatgt cttccagtct caagcagcgt ttaactgctg
    89461 agccggtgcg ttttgtttat cagcgcgata tgcccaatgc gctggttgaa gtgttacgcg
    89521 aaaaactgac tatttcccgc tacgactcca tcgtccccgg cggtcgttat cataatttta
    89581 aagactttat taatttcccc aatgtcggca aagccaatct ggtgaacaaa ccactgccgc
    89641 gtttacgcca tatttggttt gataaagccc agttccgcaa tggttttgat gccattcgcg
    89701 aacgcgatgt gttgctctat tatccttatc acacctttga gcatgtgctg gaactgctgc
    89761 gtcaggcttc gttcgacccg agcgtactgg cgattaaaat taacatttac cgcgtggcga
    89821 aagattcacg catcatcgac tcgatgatcc acgccgcaca taacggtaag aaagtcactg
    89881 tggtggttga gttacaggcg cgtttcgacg aagaagccaa cattcactgg gcgaagcgcc
    89941 tgaccgaagc aggcgtgcac gttatcttct ctgcgccggg gctgaaaatt cacgccaaac
    90001 tgttcctgat ttcacgtaaa gaaaacggtg aagtggtccg ttacgcacac atcgggaccg
    90061 ggaactttaa cgaaaaaacc gcgcgtcttt atactgacta ttcgttgctg accgcagatg
    90121 cgcgtatcac caacgaagta cggcgggtat ttaactttat tgaaaaccca taccgcccgg
    90181 tgacatttga ttatttaatg gtgtcaccgc aaaactctcg ccgtctgtta tatgaaatgg
    90241 tagaccgcga aatcgccaac gcgcagcaag ggctgcccag tggtatcacc ctgaagctaa
    90301 ataaccttgt cgataaaggc ctggttgatc gtctgtatgc ggcctccagc tccggcgtac
    90361 cggttaatct gctggttcgc ggaatgtgtt cgctgatccc caatctggaa ggcattagcg
    90421 acaacattcg tgccatcagt attgttgacc gttaccttga acatgaccgg gtttatattt
    90481 ttgaaaatgg cggcgataaa aaggtctacc tttcttccgc cgactggatg acgcgcaata
    90541 ttgattatcg tattgaagtg gcgacaccgc tgctcgatcc gcgcctgaag cagcgggtgc
    90601 tggacatcat cgacatattg ttcagcgata cggtcaaagc acgttatatc gataaagaac
    90661 tcagtaatcg ctacgttccc cgcggcaatc gccgcaaagt acgggcgcag ttggcgattt
    90721 acgactacat caaatcactc gaacaacctg aataacccta tgccaataca cgataaatcc
    90781 cctcgtccgc aggagtttgc tgcggtcgat cttggttcaa acagttttca catggtcata
    90841 gcccgtgtgg tagatggtgc catgcagatt attggccgcc tgaaacagcg ggtgcatctg
    90901 gcggacggcc tggggccaga taatatgttg agtgaagagg caatgacgcg cggtttgaac
    90961 tgtctgtcgc tgtttgccga acggctacaa gggttttctc ctgccagcgt ctgtatagtc
    91021 ggcacccata cgctgcgtca ggcgctgaac gccactgact ttctgaaacg cgcggaaaag
    91081 gtcattccct acccgattga aattatttcc ggtaatgaag aagcccgtct gatttttatg
    91141 ggcgtggaac atacccaacc ggaaaaaggt cgcaaactgg ttattgatat tggcggcgga
    91201 tctacggaac tggtgattgg tgaaaatttc gaacctattc tcgttgaaag ccgccggatg
    91261 ggttgtgtca gctttgccca gctttatttt cctggcgggg tcatcaataa agagaatttt
    91321 cagcgcgctc gcatggcggc agcacaaaaa ctggaaactt taacctggca attccgtatt
    91381 cagggctgga acgttgcaat gggcgcttcc ggtaccataa aagccgccca tgaagtgtta
    91441 atggaaatgg gcgagaaaga cgggataatt accccggaac gtctggaaaa actggtaaaa
    91501 gaaattttac gtcaccgtaa tttcgcatcg ctgagtttac cgggtctttc cgaagagcgg
    91561 aaaacagtct tcgttcctgg actggcgatt ttatgtggtg tgtttgatgc tttagccatc
    91621 cgtgaactgc gcctttctga cggggcgtta cgcgaaggcg tactgtatga aatggaagga
    91681 cgtttccgtc atcaggatgt gcgtagtcgc accgccagca gcctcgccaa ccagtatcac
    91741 atagacagcg aacaggcccg acgagtgctg gataccacta tgcaaatgta cgaacagtgg
    91801 cgggaacagc aaccgaagct ggcgcatccg caactggagg cgctactgcg atgggccgcc
    91861 atgctgcatg aggtcgggtt gaatatcaac cacagcggtt tgcatcgcca ctccgcttat
    91921 attctgcaaa acagtgactt gccgggtttt aatcaggaac agcagctgat gatggcgaca
    91981 ctggtgcgct atcaccgtaa agcgattaag ctcgacgatc tgccgcgctt taccttgttt
    92041 aagaagaaac agttcctgcc actgatacag ctattgcgcc ttggcgtatt actaaacaat
    92101 caacgtcagg caaccaccac accgccaaca ttgacactga ttactgatga cagtcactgg
    92161 acactgcgtt tcccgcatga ctggtttagt cagaatgcgc tggtactgct tgatctggaa
    92221 aaggagcaag aatactggga aggcgtggct ggctggcggt tgaaaattga agaagaaagt
    92281 accccagaaa tcgccgctta atgctttgcg ggccgacgag aaatgtcggc ccgcattatt
    92341 caggcacttt cgcgaatggg ttcgatttca ttcagcgtat caattaacgg ttgcggctta
    92401 ccaataagat aaccctgcat ataatcgatc cccaaagaga gcaccgcctc gcggatctct
    92461 tcgttttcaa cgtactctgc cactacccgc attttcttca ttcgcgccag atggcaaatc
    92521 gacgccacaa tctgataatc cagactattt gacacaatat tgcggataaa gctgccgtca
    92581 attttaagca gatcggcatt cacattttta agccgtgcat agctggcgta gccggtgccg
    92641 aaatcatcaa tcgcaatctg gcagcctaat tcctgaagat gctgcaaggt gatttgcgcc
    92701 tgcttaacat tggtcagagc attactttcg gtgacttcaa aaataagttg ccacgcttca
    92761 atctgatatt tagccagcaa ctgactgact tcaacaggaa aacgagcctg acataccgag
    92821 gttggagaca gattaatagc aaaacggtga gcgggcatct tcgctctgtt ttcagccata
    92881 aattgcagcg tatgctcaat gacccacatg tcgatactcg acgataaacc aaattcgtgc
    92941 gcgaccggca agaagctacc ggggccgatc agttcatcat tctcaccttt catgcgcaga
    93001 agaatttcat ggtaaacatc accacgcata ccggtaatcg gctgggccat caggaaaaaa
    93061 tggttgtgtt ccagcgcctg ctgtagtcga ttcatcatcg cgactttatc tttcaattcg
    93121 cgttgcaaat acattgcccc gcaacgctgc atattttccg gggcgttggt c
//
LOCUS       DAJVQR010000003        75510 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVQR010000003 DAJVQR010000000
VERSION     DAJVQR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(322..528)
                     /locus_tag="OQM52_000199"
     CDS             complement(322..528)
                     /locus_tag="OQM52_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1562200.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            841..930
                     /gene="kdpF"
                     /locus_tag="OQM52_000200"
     CDS             841..930
                     /gene="kdpF"
                     /locus_tag="OQM52_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1562201.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            930..2603
                     /gene="kdpA"
                     /locus_tag="OQM52_000201"
     CDS             930..2603
                     /gene="kdpA"
                     /locus_tag="OQM52_000201"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1562202.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2626..4673
                     /gene="kdpB"
                     /locus_tag="OQM52_000202"
                     /pseudo
     CDS             2626..4673
                     /gene="kdpB"
                     /locus_tag="OQM52_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4682..5254
                     /gene="kdpC"
                     /locus_tag="OQM52_000203"
     CDS             4682..5254
                     /gene="kdpC"
                     /locus_tag="OQM52_000203"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS1562203.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5247..7927
                     /gene="kdpD"
                     /locus_tag="OQM52_000204"
                     /pseudo
     CDS             5247..7927
                     /gene="kdpD"
                     /locus_tag="OQM52_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7924..8609
                     /gene="kdpE"
                     /locus_tag="OQM52_000205"
                     /pseudo
     CDS             7924..8609
                     /gene="kdpE"
                     /locus_tag="OQM52_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8884..8988
                     /locus_tag="OQM52_000206"
     CDS             8884..8988
                     /locus_tag="OQM52_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS1562204.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            9299..11496
                     /gene="speF"
                     /locus_tag="OQM52_000207"
                     /pseudo
     CDS             9299..11496
                     /gene="speF"
                     /locus_tag="OQM52_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11493..12812
                     /gene="potE"
                     /locus_tag="OQM52_000208"
     CDS             11493..12812
                     /gene="potE"
                     /locus_tag="OQM52_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1562205.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEK
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12868..14508)
                     /gene="pgm"
                     /locus_tag="OQM52_000209"
     CDS             complement(12868..14508)
                     /gene="pgm"
                     /locus_tag="OQM52_000209"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1562206.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14534..15079)
                     /gene="seqA"
                     /locus_tag="OQM52_000210"
     CDS             complement(14534..15079)
                     /gene="seqA"
                     /locus_tag="OQM52_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1562207.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15264..16028
                     /gene="ybfF"
                     /locus_tag="OQM52_000211"
     CDS             15264..16028
                     /gene="ybfF"
                     /locus_tag="OQM52_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1562208.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16168..16461
                     /gene="ybfE"
                     /locus_tag="OQM52_000212"
     CDS             16168..16461
                     /gene="ybfE"
                     /locus_tag="OQM52_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1562209.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16601..17131
                     /gene="fldA"
                     /locus_tag="OQM52_000213"
     CDS             16601..17131
                     /gene="fldA"
                     /locus_tag="OQM52_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1562210.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17341..17427
                     /gene="uof"
                     /locus_tag="OQM52_000214"
     CDS             17341..17427
                     /gene="uof"
                     /locus_tag="OQM52_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1562211.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17420..17866
                     /gene="fur"
                     /locus_tag="OQM52_000215"
     CDS             17420..17866
                     /gene="fur"
                     /locus_tag="OQM52_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1562212.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17950..18276)
                     /locus_tag="OQM52_000216"
     CDS             complement(17950..18276)
                     /locus_tag="OQM52_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562213.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18313..19719)
                     /gene="chiP"
                     /locus_tag="OQM52_000217"
     CDS             complement(18313..19719)
                     /gene="chiP"
                     /locus_tag="OQM52_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1562214.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20298..21962)
                     /gene="glnS"
                     /locus_tag="OQM52_000218"
     CDS             complement(20298..21962)
                     /gene="glnS"
                     /locus_tag="OQM52_000218"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1562215.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22165..24111)
                     /gene="nagE"
                     /locus_tag="OQM52_000219"
     CDS             complement(22165..24111)
                     /gene="nagE"
                     /locus_tag="OQM52_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1562216.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24444..25244
                     /gene="nagB"
                     /locus_tag="OQM52_000220"
     CDS             24444..25244
                     /gene="nagB"
                     /locus_tag="OQM52_000220"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1562217.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25304..26452
                     /gene="nagA"
                     /locus_tag="OQM52_000221"
     CDS             25304..26452
                     /gene="nagA"
                     /locus_tag="OQM52_000221"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1562218.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26461..27681
                     /gene="nagC"
                     /locus_tag="OQM52_000222"
                     /gene_synonym="nagR"
     CDS             26461..27681
                     /gene="nagC"
                     /locus_tag="OQM52_000222"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1562219.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27729..28481
                     /gene="nagD"
                     /locus_tag="OQM52_000223"
                     /pseudo
     CDS             27729..28481
                     /gene="nagD"
                     /locus_tag="OQM52_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28878..30542
                     /gene="asnB"
                     /locus_tag="OQM52_000224"
     CDS             28878..30542
                     /gene="asnB"
                     /locus_tag="OQM52_000224"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1562220.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            30921..30997
                     /locus_tag="OQM52_000225"
     tRNA            30921..30997
                     /locus_tag="OQM52_000225"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30955..30957,aa:Met,seq:cat)
     gene            31006..31090
                     /locus_tag="OQM52_000226"
     tRNA            31006..31090
                     /locus_tag="OQM52_000226"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31040..31042,aa:Leu,seq:tag)
     gene            31114..31188
                     /locus_tag="OQM52_000227"
     tRNA            31114..31188
                     /locus_tag="OQM52_000227"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31146..31148,aa:Gln,seq:ttg)
     gene            31223..31297
                     /locus_tag="OQM52_000228"
     tRNA            31223..31297
                     /locus_tag="OQM52_000228"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31255..31257,aa:Gln,seq:ttg)
     gene            31313..31389
                     /locus_tag="OQM52_000229"
     tRNA            31313..31389
                     /locus_tag="OQM52_000229"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31347..31349,aa:Met,seq:cat)
     gene            31438..31512
                     /locus_tag="OQM52_000230"
     tRNA            31438..31512
                     /locus_tag="OQM52_000230"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31470..31472,aa:Gln,seq:ctg)
     gene            31550..31624
                     /locus_tag="OQM52_000231"
     tRNA            31550..31624
                     /locus_tag="OQM52_000231"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31582..31584,aa:Gln,seq:ctg)
     gene            complement(31778..32953)
                     /gene="ubiF"
                     /locus_tag="OQM52_000232"
     CDS             complement(31778..32953)
                     /gene="ubiF"
                     /locus_tag="OQM52_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1562221.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33099..34523
                     /gene="miaB"
                     /locus_tag="OQM52_000233"
     CDS             33099..34523
                     /gene="miaB"
                     /locus_tag="OQM52_000233"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1562222.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34676..35716
                     /locus_tag="OQM52_000234"
     CDS             34676..35716
                     /locus_tag="OQM52_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1562223.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35713..36180
                     /gene="ybeY"
                     /locus_tag="OQM52_000235"
     CDS             35713..36180
                     /gene="ybeY"
                     /locus_tag="OQM52_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1562224.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36270..37148
                     /gene="corC"
                     /locus_tag="OQM52_000236"
     CDS             36270..37148
                     /gene="corC"
                     /locus_tag="OQM52_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1562225.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37173..38711
                     /gene="lnt"
                     /locus_tag="OQM52_000237"
     CDS             37173..38711
                     /gene="lnt"
                     /locus_tag="OQM52_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1562226.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39108..40016
                     /gene="gltI"
                     /locus_tag="OQM52_000238"
     CDS             39108..40016
                     /gene="gltI"
                     /locus_tag="OQM52_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1562227.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40186..40926
                     /gene="gltJ"
                     /locus_tag="OQM52_000239"
     CDS             40186..40926
                     /gene="gltJ"
                     /locus_tag="OQM52_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1562228.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            40926..41600
                     /gene="gltK"
                     /locus_tag="OQM52_000240"
     CDS             40926..41600
                     /gene="gltK"
                     /locus_tag="OQM52_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1562229.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41600..42324
                     /gene="gltL"
                     /locus_tag="OQM52_000241"
                     /pseudo
     CDS             41600..42324
                     /gene="gltL"
                     /locus_tag="OQM52_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
     gene            42442..43377
                     /gene="rihA"
                     /locus_tag="OQM52_000242"
     CDS             42442..43377
                     /gene="rihA"
                     /locus_tag="OQM52_000242"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1562230.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWLGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43461..45131
                     /gene="hscC"
                     /locus_tag="OQM52_000243"
                     /pseudo
     CDS             43461..45131
                     /gene="hscC"
                     /locus_tag="OQM52_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45191..46642)
                     /gene="djlC"
                     /locus_tag="OQM52_000244"
     CDS             complement(45191..46642)
                     /gene="djlC"
                     /locus_tag="OQM52_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS1562231.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46639..47346)
                     /locus_tag="OQM52_000245"
     CDS             complement(46639..47346)
                     /locus_tag="OQM52_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1562232.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47448..48002
                     /locus_tag="OQM52_000246"
     CDS             47448..48002
                     /locus_tag="OQM52_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1562233.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48012..49439)
                     /locus_tag="OQM52_000247"
     CDS             complement(48012..49439)
                     /locus_tag="OQM52_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1562234.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49436..50143)
                     /locus_tag="OQM52_000248"
     CDS             complement(49436..50143)
                     /locus_tag="OQM52_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1562235.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50307..51284
                     /locus_tag="OQM52_000249"
     CDS             50307..51284
                     /locus_tag="OQM52_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1562236.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51354..51836)
                     /locus_tag="OQM52_000250"
     CDS             complement(51354..51836)
                     /locus_tag="OQM52_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1562237.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52071..54653
                     /gene="leuS"
                     /locus_tag="OQM52_000251"
     CDS             52071..54653
                     /gene="leuS"
                     /locus_tag="OQM52_000251"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1562238.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54668..55249
                     /gene="lptE"
                     /locus_tag="OQM52_000252"
     CDS             54668..55249
                     /gene="lptE"
                     /locus_tag="OQM52_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1562239.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55249..56280
                     /gene="holA"
                     /locus_tag="OQM52_000253"
     CDS             55249..56280
                     /gene="holA"
                     /locus_tag="OQM52_000253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1562240.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56282..56923
                     /gene="nadD"
                     /locus_tag="OQM52_000254"
     CDS             56282..56923
                     /gene="nadD"
                     /locus_tag="OQM52_000254"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1562241.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            56947..57558
                     /locus_tag="OQM52_000255"
     CDS             56947..57558
                     /locus_tag="OQM52_000255"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCS1562242.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57818..58135
                     /gene="rsfS"
                     /locus_tag="OQM52_000256"
                     /gene_synonym="rsfA"
     CDS             57818..58135
                     /gene="rsfS"
                     /locus_tag="OQM52_000256"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1562243.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58139..58606
                     /gene="rlmH"
                     /locus_tag="OQM52_000257"
     CDS             58139..58606
                     /gene="rlmH"
                     /locus_tag="OQM52_000257"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1562244.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58637..60538
                     /gene="mrdA"
                     /locus_tag="OQM52_000258"
     CDS             58637..60538
                     /gene="mrdA"
                     /locus_tag="OQM52_000258"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1562245.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60541..61653
                     /gene="mrdB"
                     /locus_tag="OQM52_000259"
                     /gene_synonym="rodA"
     CDS             60541..61653
                     /gene="mrdB"
                     /locus_tag="OQM52_000259"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1562246.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61664..62752
                     /gene="rlpA"
                     /locus_tag="OQM52_000260"
     CDS             61664..62752
                     /gene="rlpA"
                     /locus_tag="OQM52_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1562247.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYVLPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62891..64102
                     /gene="dacA"
                     /locus_tag="OQM52_000261"
     CDS             62891..64102
                     /gene="dacA"
                     /locus_tag="OQM52_000261"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1562248.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64213..64476
                     /locus_tag="OQM52_000262"
     CDS             64213..64476
                     /locus_tag="OQM52_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1562249.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64577..65218
                     /gene="lipB"
                     /locus_tag="OQM52_000263"
     CDS             64577..65218
                     /gene="lipB"
                     /locus_tag="OQM52_000263"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1562250.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65477..66430
                     /locus_tag="OQM52_000264"
     CDS             65477..66430
                     /locus_tag="OQM52_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1562251.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66639..67604
                     /gene="lipA"
                     /locus_tag="OQM52_000265"
     CDS             66639..67604
                     /gene="lipA"
                     /locus_tag="OQM52_000265"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1562252.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67705..67908)
                     /gene="tatE"
                     /locus_tag="OQM52_000266"
     CDS             complement(67705..67908)
                     /gene="tatE"
                     /locus_tag="OQM52_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1562253.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68037..68825)
                     /locus_tag="OQM52_000267"
     CDS             complement(68037..68825)
                     /locus_tag="OQM52_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1562254.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            68918..69301
                     /gene="crcB"
                     /locus_tag="OQM52_000268"
     CDS             68918..69301
                     /gene="crcB"
                     /locus_tag="OQM52_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1562255.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69355..69564)
                     /gene="cspE"
                     /locus_tag="OQM52_000269"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69355..69564)
                     /gene="cspE"
                     /locus_tag="OQM52_000269"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1562256.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69739..70299)
                     /gene="pagP"
                     /locus_tag="OQM52_000270"
     CDS             complement(69739..70299)
                     /gene="pagP"
                     /locus_tag="OQM52_000270"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1562257.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70888..72273
                     /gene="dcuC"
                     /locus_tag="OQM52_000271"
     CDS             70888..72273
                     /gene="dcuC"
                     /locus_tag="OQM52_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1562258.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72314..72655)
                     /locus_tag="OQM52_000272"
                     /pseudo
     CDS             complement(72314..72655)
                     /locus_tag="OQM52_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(72993..73502)
                     /locus_tag="OQM52_000273"
     CDS             complement(72993..73502)
                     /locus_tag="OQM52_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1562259.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73665..73910
                     /locus_tag="OQM52_000274"
     CDS             73665..73910
                     /locus_tag="OQM52_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1562260.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73885..>75510
                     /locus_tag="OQM52_000275"
     CDS             73885..>75510
                     /locus_tag="OQM52_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HCS1562261.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAEGVAMFNART
                     GRETEMCGGKLSFDDVFEREYARTIVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGG
                     SLKGAKNVYYNMALMNAGVKKVVVRFDPQQLHST"
BASE COUNT        18576 a        18977 c        19193 g        18764 t
ORIGIN      
        1 aaaattcgga atttatttag atgctggaat tagaaaaagg gatgacggct ttctcacctt
       61 accctgaagt ttcacgaaag tgtgtaagga agtatgcaat ctaactggag atttctgatg
      121 atctagtcgc ttagagcgac aatgaaggtg accgcatcct gggtagccct atgaaaggat
      181 actattgaaa cgtgatgcta aaacagtgat gaaatgaata atacgatgga aacgtcttcc
      241 atgttaatta tcatggaaga cgttgatgtt gaacacctaa accagccccc tgatacgagg
      301 ctggcttaag cgcgcaggca atcagtaaaa atcaccggtt gccttttcgg cctgatccat
      361 ccacaccggt ttatcgctgg ttttcgacca gacgcggtgc aggtagctgt aaaagcgggc
      421 gcggtctttc cagaaaagca tgaaaggcag cgccagaacg cccgctgcaa ccgcatatgt
      481 acggcgtaaa aagataagcc atgcaggata ttctctgtag agttccatac ttttctcccc
      541 cttttgtatc tacccggtga atagcaccgg aaaaatgaat ttgtttatct gatgaaaata
      601 gtaccgcctt ttgtgtaatt ttactactca tccgaccact tatttttgct tattgatggt
      661 ttatttacat tcacactgta attaaattac acaaaagtta aattaatact aaccatcagt
      721 taaatcatgg cctttgccat ttttatactt tttttacacc ccgtccgcag atttttgcga
      781 aatctttgca gccagaattc tactcttccg gtatcacttt taggccactg gaggtgcact
      841 gtgagtgcag gcgtgatagc cggcgtgttg ctggtgtttt tattactggg ctatttggtt
      901 tatgccctga tcaatgcgga ggcgttctga tggctgcgca agggttctta ctgattgcca
      961 cgtttttact ggtattaatg gtgctggcgc gtcctttagg cagcgggctg gcgcggctga
     1021 ttaatgacat tcctcttcca ggtacagcgg gcgttgaacg tatactcttt cgtttacctg
     1081 gcgtctctga ccatgagatg aactggaagc aatatctttg tgccattctc ggcctgaaca
     1141 tgctggggct ggcggtgctg ttttttatgt tgctcggtca gcactatctg ccgcttaatc
     1201 cacagcagtt gccggggctg tcgtgggatc tggcgctgaa taccgccgtc agctttgtca
     1261 cgaataccaa ctggcaatct tatagcggtg aaaccacgtt gagctatttc agccagatgg
     1321 caggcttaac ggtacaaaac tttctttctg ccgccagcgg gattgcggtg atttttgcct
     1381 tcatccgtgc gtttactcgc cagagcatga gcacactcgg gaatgcctgg gtcgatctgc
     1441 tacgcatcac gttatgggtg ctagtccctg tggcgttgtt gatcgcactg ttttttattc
     1501 aacaaggtgc gcagcaaaac tttctgccct atcaggcagt gaataccgtt gaaggagcgc
     1561 aacagctgtt acccatggga ccagtagctt ctcaggaagc gatcaagatg ctcggtacta
     1621 acggcggtgg cttctttaat gccaactcgt cgcatccgtt tgaaaaccca accgcactga
     1681 ccaacttcgt gcagatgctg gcgatcttct tgatcccaac agcgctgtgc tttgcctttg
     1741 gtgaagtgac gggcgatcgc cgccaggggc gcatgttgct gtgggcgatg tcagtgattt
     1801 ttgtcatctg cgtaggcgtg gttatgtggg cagaagttca gggtaatcct catctgctgg
     1861 cgctcggcgc agacagcagc atcaatatgg aaggtaaaga gagccgtttc ggcgtgctgg
     1921 tcagtagcct gtttgcggtc gtgactacgg cggcgtcctg tggcgcggtg attgcgatgc
     1981 atgattcgtt taccgctctc ggcggcatgg tgccgatgtg gctaatgcaa attggtgaag
     2041 tggtgttcgg cggtgtcggt tctggtcttt acggcatgat gctgtttgtc ctgctggcgg
     2101 tgtttattgc cgggctgatg attggtcgta caccggaata tctgggtaaa aaaatagacg
     2161 tacgcgagat gaaactgact gcactggcga ttctggttac tccaacactg gtactgatgg
     2221 gcgcggcgtt ggcgatgatg accgacgccg gacgtagcgc catgcttaac cctagcccgc
     2281 atggttttag cgaagtgctg tacgccgtgt cgtccgccgc taacaacaac ggcagcgcct
     2341 ttgccggatt aagcgccaac tctccgttct ggaactgttt actggcgttc tgcatgtttg
     2401 tcggtcgctt cggggtgatt atcccggtga tggcaattgc cggttcgctg gtgagtaaaa
     2461 agagccaacc cgccagctcc ggcacgctgc caacgcacgg cccgctgttt gttggcctgt
     2521 taatcggcac cgtgttgctg gttggcgcac tgacctttat ccctgccctg gcgcttggtc
     2581 cggtggcgga atatctctcc tgatgatatt gagtgagcac tgaatatgag tcgtaaacaa
     2641 ctggcgctat tcgaaccaac acttgtcgtt caggcgctga aagaagcggt gaaaaaatta
     2701 aacccgcagg cgcaatggcg caatccggtg atgtttatcg tctggatcgg cagtctgctg
     2761 accacctgta ttagcatcgc gatggcaagc ggtgcgatgc ctggcaatgc gctgtttagc
     2821 gcggcaatta gcggctggct gtgggtcacc gtactgttcg ctaatttcgc cgaggcgctg
     2881 gcagaaggcc gcagtaaagc gcaggccaac agtcttaaag gggtgaaaaa aactgccttt
     2941 gcccgcaagc tgcgtgagcc gaaatatggc gctgcggcgg acaaagttcc tgccgatcaa
     3001 ctgcgtaaag gtgacatcgt gctggtagaa gctggcgata ttatcccctg cgatggtgaa
     3061 gtcattgaag gcggcgcatc ggtcgatgaa agcgccatca ccggggaatc ggcaccggtg
     3121 atccgtgaat ccggcggcga ttttgcctcc gttaccggcg gcacgcgtat tctttctgac
     3181 tggctggtga ttgagtgtag cgttaacccc ggtgagacat ttctggatcg gatgatcgcg
     3241 atggtggaag gcgcacagcg acgcaaaaca ccgaacgaga ttgccctgac tatcctgctg
     3301 attgctctga ccatcgtctt tttactggca accgccacgc tgtggccgtt ttccgcgtgg
     3361 ggcggtaatg ccgtgagtgt aacggtactg gtggcgctgc tggtctgtct gatcccaacc
     3421 actattggtg gcctgttgtc agcgatcggc gtcgccggga tgagtcggat gctgggcgcg
     3481 aatgtgattg ccaccagcgg acgtgcagtt gaagcggcag gtgacgttga cgttctgcta
     3541 ctggataaaa ccggcaccat cacactcggt aaccgtcagg cgtcggagtt tatccccgcg
     3601 cagggcgtgg atgaaaaaac gctggctgac gccgcacaac tggcttcgct ggctgatgaa
     3661 acgccggaag gtcgcagtat tgtgatcctc gccaagcaac gttttaacct gcgcgagcgc
     3721 gatgtgcagt cgctccatgc cacctttgta ccgtttactg cgcaaagccg gatgagcggg
     3781 atcaacatcg acaaccgcat gatccgtaaa ggttctgtcg atgccattcg ccgccatatt
     3841 gaggctaacg gcggtcactt ccctgccgat gttgatcaaa aagtcgatca ggttgcgcgt
     3901 caaggagcca cgctgctggt ggtggtggaa ggttctcgtg tgctgggcgt tattgcgctg
     3961 aaagatatcg tcaaaggcgg tattaaagag cgcttcgccc agctgcgcaa atgggcatta
     4021 aaacggtgat gattaccggc gataaccgtc tgactgccgc cgcgatagct gcggaagcgg
     4081 gtgtcgatga ttttctcgcc gaagcgacac cggaggccaa gctggcattg atccgtcagt
     4141 atcaggcgga aggtcgtttg gtagcgatga ccggcgacag caccaacgat gctacggcgc
     4201 tggcgcaggc agatgtcgcg gtggcgatga actccggcac ccaggcggct aaagaggcgg
     4261 gcaatatggt ggatctcgac tctaacccga ccaagttgat cgaggtggtg cacattggca
     4321 aacagatgct gatgacccgt ggctcgctga ccaccttcag cattgccaac gatgtggcga
     4381 aatacttcgc cattattccg gcggcgttcg cggcaacata tccgcagtta aatgcgctga
     4441 acatcatgcg cctgcattcg cccgactccg ccattctcag tgcggtcatt ttcaacgcct
     4501 tgattatcgt ctttttgatt cctctggcgt taaaaggcgt gagttataaa ccgcttaccg
     4561 cttctgccat gttgcgccgt aacttatgga tttacggtct gggtgggctg ctggtgccgt
     4621 ttattggtat caaagtcatt gatttactgc taaccgtttg tagtctggtg tgaggtttac
     4681 catgagtgga ttacgtccgg cattatcgac atttctcttt ctgttattga ttactggcgg
     4741 cgtttacccg ctgctgacca ccgcactggg gcaatggtgg tatccctggc aggccaacgg
     4801 ttcgttgatt cgtgaaggtg atacggtgcg cggttcggca ttaatcgggc aaaattttac
     4861 cggcaacggc tattttcatg gtcgcccgtc ggcaacagca gaaatgccct ataatccaca
     4921 ggcttctggc gggagcaatc tggcagtcag taacccggag ctggataaac agatagccgc
     4981 acgcgttgcc gcattacggg ccgctaaccc ggacgccagc acgaacgttc cggttgaact
     5041 ggtgacggcg tcggcaagcg gactggacaa taacatcacc ccgcaagcgg cggcctggca
     5101 gatcccacgc atcgcaaaag cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc
     5161 aaaatacagc caacaaccgc tggtgaaata tatcggccag ccggtcgtca acattgtcga
     5221 actcaatctg gcactggata agcttgatga ataacgaacc cttacgtccc gaccccgacc
     5281 gtctgctgga acaaactgcc gcgccgcatc gggggaagct gaaagttttc ttcagtgcct
     5341 gtgcaggcgt cgggaagacc tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag
     5401 ggctggatat tgtggttggt gtggtagaaa cccatgggcg gaaagatacc gccgccatgc
     5461 ttgaagggct ggctgttctg cctctgaaac gccaggcgta ccgtgggcgg catatcagcg
     5521 agtttgatct cgatgccgcc ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg
     5581 cgcacagtaa tgcgccaggt tctcgtcatc ctaaacgctg gcaggatatc gaagaactgc
     5641 tggaagctgg cattgatgtt ttcacaaccg tcaacgttca gcatctggaa agtctgaatg
     5701 atgtggtcag cggcgtcacc ggaattcagg tacgggaaac cgtgcccgat ccttttttcg
     5761 atgccgccga cgacgtggtg ctggtggact tgcccccgga cgatctgcgc cagcggctga
     5821 aagaaggcaa agtctatatt gccgggcagg cggagcgcgc cattgaacat tttttccgca
     5881 aaggcaatct gatcgccctg cgcgaactgg cactgcgccg tactgccgat cgcgttgatg
     5941 agcaaatgcg cgcctggcgg gggcatcctg gcgaagagaa agtctggcac acgcgcgacg
     6001 cgatcctttt atgcatcggc cataacaccg gcagcgaaaa ctggtccgca cggcggcgcg
     6061 gctgacgtca cggctgggga gcgtctggca cgcggtgtat gttgaaaccc ctgccctgca
     6121 tcgcttaccg gagaaaaaac gtcgggcaat tctcagcgcc ttacgtctgg cgcaggagct
     6181 gggcgcggag acggcaacac tttctgatcc agcggaagag aaagcggtag tgcgttatgc
     6241 ccgtgaacat aatctcggca agattattct cggtcgccca gcctcgcgcc gctggtggcg
     6301 tcgggaaaca tttgctgacc gactggcgcg catcgccccc gatctcgatc aggtgctggt
     6361 cgcgcttgat gaaccacccg cccgcacgat taacaacgcg ccggatagcc gctcatttaa
     6421 agacaagtgg cgggtacaga ttcagggatg cgtggttgcc gccgcgttat gcgccgttat
     6481 caccttaatt gccatgcagt ggctgatggc gtttgatgcc gccaacctgg tgatgctgta
     6541 tctgcttggc gtggtggtgg cgctatttta tggacgctgg ccttcggtgg ttgccaccgt
     6601 cattaatgtg gtgagtttcg atctcttttt tatcgcccca cgcggcacgc tcgccgtctc
     6661 tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa
     6721 ccttactgct ggtgtgcgtt atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg
     6781 gcacttatat gaaatgtcga aagctctggc ggtgggccgc agtccgcagg acatcgccgc
     6841 caccagcgaa caatttattg cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga
     6901 tgacaacggt aaattgcagc cgttaacaca tccgcaagga attacgccat gggacgatgc
     6961 catcgcgcag tggagttttg ataaaggcct gcctgcgggc gcgggcaccg acacgttacc
     7021 cggtgtaccg taccagattt tgccgctaaa aagcggcgag aaaacctacg ggctggtggt
     7081 ggtggagccg gggaatctgc gccagttgat gatcccggaa cagcagcgcc tgctggagac
     7141 ttttacgctg ttagtcgcca atgcccttga gcggctgacg ctaaccgcca gcgaagaaca
     7201 ggcgcggatg gcaagcgaac gtgaacagat ccgcaacgcc ctgctggcag cgctttcgca
     7261 tgatttacgc acgccgctta cggtgctgtt tggtcaggca gaaatcttaa cgctcgatct
     7321 ggcaagcgaa ggatcacccc acgcccgcca ggccagcgag attcgtcagc atgtgctgaa
     7381 cactacccga ctggtgaata atctactgga tatggcgcga attcagtccg gcggctttaa
     7441 tttgaagaaa gagtggttaa cgctggaaga agtagtcggc agcgcgctgc aaatgccgga
     7501 accgggttta tcgtcgccca tcaatctttc tctgccagaa ccgctgacct taatccacgt
     7561 tgacgggcca ctctttgaac gggtgctgat taatctgctg gagaacgcgg tgaaatatgc
     7621 gggtgcgcag gccgaaattg gtatcgatgc ccacgttgag ggcgaaaatc tacaactgga
     7681 tgtctgggat aacggccccg gtcttccgcc aggccaggag cagacgatat ttgataagtt
     7741 tgctcgcggg aataaagagt cggcggtgcc gggtgtcggg cttgggttgg caatttgtcg
     7801 ggcgattgtg gatgtacacg gcggcaccat taccgcgttc aaccgaccgg aaggtggtgc
     7861 ctgttttcgt gttacacttc cccagcaaac cgctcctgaa cttgaagact ttcatgagga
     7921 tatgtgacaa acgttctgat tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg
     7981 gcgctggagg gcgacgggat gcgcgtcttt gaggccgaaa ctctgcaacg cggcttgctg
     8041 gaagcggcaa cccgtaagcc agatttgatt attctcgatc tcggcctgcc cgatggtgat
     8101 gggattgagt ttatccgcga cctgcgccag tggagcccgg tgccggtgat tgtgctttcc
     8161 gcacgcagcg aagagagcga caagatcgcc gcgctggatg ccggagcgga tgattatctg
     8221 agtaagccgt ttggtattgg cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac
     8281 tccgccactc cgccaccgcc gcgcccgatc cgctggtgaa attttccggt gttaccgtcg
     8341 atttagccgc ccgcgtgatt caccggggtg aggaagaggt gcatctcaca ccaattgagt
     8401 tccgcctgct ggcggtgctg ctcaacaatg ccggaaaagt actcacccag cgccagctcc
     8461 ttaaccaggt gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg
     8521 gacatctgcg acaaaaactg gaacaggatc cggcccggcc tcgccacttc attactgaaa
     8581 ccggtattgg gtatcggttt atgctttgaa ttgttattca ataaattcgc ttaaatagtg
     8641 attaatttat tattaaacct gcgccgaata ttattatttg gcaggtttaa tttcttaacg
     8701 caaatataaa acacgaaatt acaacattta agtaacaaaa cagcatctaa tatggcgata
     8761 ttcataatta atgatttatt atctggggta tgatcgtttt ttgttgatct tcttcacaga
     8821 ttatagccat ttcatggata gaataactct accttcaact gacacagcaa gcggtaaagg
     8881 taaatggaaa ataataaccg cttaatgcct catataaggc ggacaaccca tatcatgaag
     8941 tttacccatc gtaatagttt tgactttcat ttctttaatg cccgctagtc ttctgactaa
     9001 agggcacccc aacgtacagg tctccctgac tttaagcatt acaggttaat acctgtattc
     9061 ctcggtgctc atatactgct aaccctttta aactctaaat aattcgagtc gcagcacttg
     9121 caacttgagg tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca
     9181 aaaagcaaac tactgattta tttatcagcg gtggagcttt gctttttttc cgacgtgatc
     9241 gatttctcct ttgagaaatt gaggacctgc tattacctga aataaagaga tgaataaaat
     9301 gtcagaatta aaaattgccg ttagccgttc ttgcccggat tgtttttcca ctcatcgtgc
     9361 atgcgtgaat attgacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcaa
     9421 tgatgttgaa cgtggaaaac tcgataaaat agacgctact ggctatggca ttcctgtttt
     9481 tattgcaacg gaaaatgaag aacgtgtacc agcagaatat ctgccacgta tttctggtgt
     9541 cttcgagcat tgcgaatcac ggaaagagtt ttatggacgt cagttagaaa ccgctgctag
     9601 ccattatgaa acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgtcaatca
     9661 gggcaatagc gcttttgact gcccgggtca tcagggaggt gagtttttcc gtcgtcatcc
     9721 ggcagggaat cagttcgtgg agtacttcgg agaaacgctg ttccgttccg acttatgcaa
     9781 cgcagacgta gcaatgggcg atctgctgat tcacgaaggc gcgccatgca ttgcacagca
     9841 acatgcggca aaagtgttta atgccgataa aacatacttc gttctaaatg gcacttcatc
     9901 ttctaacaaa gtggttttaa acgccctgct aacaccgggt gatctggtgc tgtttgatcg
     9961 caataaccac aaatctaacc accacggagc gttgctacag gctggtgcaa caccggttta
    10021 tctggaaacg gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga
    10081 agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg cagcgggcaa aagaggctcg
    10141 tcctttccgc ctcgctgtga ttcagttagg cacctacgac ggtacgattt ataacgcccg
    10201 ccaggtggtg gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt
    10261 tggctatgag caatttatcc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa
    10321 tgagaacgat ccgggtattc tagttacgca atccgtacat aaacagcagg ctggtttttc
    10381 tcaaacttca caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc
    10441 gcacaaacgc atgaacaacg cctttatgat gcacgcctcc accagcccgt tctatccgct
    10501 gtttgccgca ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg
    10561 gatggattgt gtggtaaatg gcattaatgc ccgcaaactg atcctcgata actgtcagca
    10621 tattcgtccg ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc
    10681 gcaaattgcg gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcattcttt
    10741 tgaaggctat gcagagaatc aatactttgt cgatccttgc aaactgttgc tgacaacccc
    10801 aggtattgat gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc
    10861 taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaacg ccatcctctt
    10921 cctgctgact ccggcagaag atatggccaa acttcagcaa cttgtgccct gctggtacgc
    10981 ttcgaaaaac tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa
    11041 cagcatgaag agcgctacgc cggttatacc ctgcgtcagt tgtgccagga aatgcatgat
    11101 ttgtatgccc gccacaacgt gaaacaactg caaaaagaga tgttccgcaa agcgcacttc
    11161 ccacacgtca gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag
    11221 ctggttcgtc taccggatgc cgaaggtcgt atcgctgccg aaggggcgct tccttatcct
    11281 ccgggtgtgc tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc
    11341 agcgctctgg aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc
    11401 tatatcgaag aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat
    11461 gcgcaaagca ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg
    11521 gcgtcgttca gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc
    11581 tgccgacaaa gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg
    11641 taggctcaat ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag
    11701 gtggtatggg cggttatgcc gagtatgctt tcggtaaatc cggtaacttt attgcgaact
    11761 atacctacgg cgtctcactg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt
    11821 acggcaccga actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg
    11881 gggtgctgtg gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca
    11941 gtagcattac cgtgtggggg ggcattattc cggtggtagg cctgtgcatt atcggctggt
    12001 tctggtttag cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca
    12061 gtgcagtagg ttcttccatc gctatgacgc tgtgggcttt tcttggtctg gagtctgcgt
    12121 gtgcgaatac tgatgtagtg gaaaaaccgg aacgtaatgt gccaatcgcg gtactcggcg
    12181 gtacgttagg tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc
    12241 ctaatatgga gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttta
    12301 caccagaagt gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc
    12361 ttggctggca gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc
    12421 ctaaaatttt ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg
    12481 tgattatcca gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca
    12541 acgtgctggt taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg
    12601 cattagtcat tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact
    12661 ttgttgcttt tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag
    12721 ccatgctgta cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac
    12781 cacgctttga actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg
    12841 cgaccgctct ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc
    12901 aaccgcttct ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat
    12961 cttatatgcg tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac
    13021 tttcagaccg ccaatagaag caccgttgcc cggagcagca gtcaggcgcg cggtgatcgg
    13081 gtcacctgcc agggtgctgg cgctcaccat ttccggagac agcttagaca gcgccgcttt
    13141 ttgtgcggaa gtcgcagctg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc
    13201 cagttcgttg tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag
    13261 cagacacata atgatgccgt ctttgtcggt ggaccacggc gtgccgtcga aacgcaggaa
    13321 ggaagcccct gcgctctctt cgccgccaaa gccgaagctg ccgtcgaaca gaccatcaac
    13381 aaaccatttg aaacctaccg gtacttctac cagcttacgg cccaagtcat tgaccacacg
    13441 gtcgatcatc gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg
    13501 acgatgctgg aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc
    13561 tgccggagtg acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa
    13621 cttatcacgc agtgccagca gacccgccat cgcacactcg gaggagcagt ccatacggat
    13681 cgcaccgtct ttatcgaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat
    13741 ggtcaggttg aggttgtaat actcgccaat gcgcttccag tattcgatac cggaaccgcc
    13801 caacggatca acgcccagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc
    13861 ggccagccct tccacgaacg gctgcaccag atcctgctct ttcacatagc cggatgccat
    13921 cgcttcgtcg agggagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc
    13981 cttgtcttcc accactttgg tgacgttagt atcagccggg ccaccatttg gcggattgta
    14041 cttgatacca ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag
    14101 cgggccacct tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa
    14161 gccattgttt tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga
    14221 aatgaacgca ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat
    14281 gccgtttttc gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt
    14341 aaagctgtgg cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc
    14401 cgcattccct gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat
    14461 caaatcactc tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt
    14521 tgtctgcaac gttttagata gttccgcaaa ccttctcaat caattccgcc gggaattgca
    14581 tcgactgcat gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca
    14641 cccaatacgg cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg
    14701 tttgttcatc tgccgcaaag tacacgcgtg tacgaccgtg caacgattcc gttgcttcgg
    14761 caaacgcctg ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg
    14821 ctcgcttttg ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt
    14881 ctttaatcgt tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt
    14941 tcgtcaccgg agcagcaggc tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt
    15001 cggatgcgct ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat
    15061 caacttcaat cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccaa
    15121 gagtatatga ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg
    15181 aataaacctt atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac
    15241 aacgcgaaaa aaagaacttt gccatgaaat taaatatccg cgcgcaaact gcacaaaacc
    15301 agcacaataa ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg
    15361 gcgtactggc tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc
    15421 acggtctttc accgagagag ccggtaatga attacccggc gatggcgcag gatcttgttg
    15481 ataccctgga tgcactgcag atcgacaaag caacatttat cggtcactcc atgggcggta
    15541 aagcggtaat ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg
    15601 atatcgcacc ggtcgactat cacgtacgcc gtcatgatga gattttcgca gctatcaacg
    15661 cggtcagtga atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc
    15721 ttaatgaaga aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct
    15781 ttaacgtgcc ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg
    15841 catgggatca ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt
    15901 accgtgatga tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg
    15961 gtcactgggt ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca
    16021 atgattaacg caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg
    16081 gcgtgactca tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg
    16141 attgtttccc ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta
    16201 gatctgttcg cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat
    16261 gaacagctgc gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag
    16321 cgtgtcgaac tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggaatcg
    16381 cgtaatatga gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg
    16441 cgtagtcagg gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg
    16501 tttgctgatt ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt
    16561 ccacccccat ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt
    16621 tttcggcagc gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg
    16681 taaagacgtt gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta
    16741 cgacattctg ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga
    16801 tgacttcttc ccgactctcg aagagattga tttcaatggt aaactggttg cgctgtttgg
    16861 ttgtggtgac caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga
    16921 catcattgaa ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt
    16981 cgaagcatca aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga
    17041 ccgtcagccg gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga
    17101 gttgcatctc gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc
    17161 cgtattaata gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt
    17221 acctatacaa tgtcccagtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg
    17281 tttttattaa caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata
    17341 atgatacgca ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta
    17401 gtaacaggac agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa
    17461 agtaacgctt cctcgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt
    17521 cagtgcggaa gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac
    17581 ggtatatcgc gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga
    17641 aggcggtaaa tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct
    17701 cgactgcggc aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat
    17761 tgccgcaaaa catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc
    17821 cgaaggcgat tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga
    17881 gaaaagccaa cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac
    17941 agctctcact tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt
    18001 caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg
    18061 tacactttcc tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac
    18121 gctctggcag gcttcaattt tttctggcga accttgcgcg gtgttgatac aggcgctgta
    18181 tgcctctttc agacggctgt cttcctgtgg tgcggttgat tgcgcacaag ctaccagccc
    18241 cgatgccatc atggcgatga gaatcagttt tttcattgaa tgcggcaggc acaaaacctg
    18301 ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcacgctc
    18361 atcctggaag atgttaccgt aaccgccgcc ccagcttggg atgtcggagt ggttgtcgta
    18421 ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtgtagac
    18481 cgcatccagg ctgtaggcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg
    18541 attgctctgc caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc
    18601 gaagcctgga agattccagt ttttcaggtc atacatcgca ctgaagaaga ccgctttttc
    18661 gccgttagcg ttgaagtcgg aacggttatc ccaccagata tccaggcggc cgttggagga
    18721 agcgtaggtt ggggtcatac gttgcaggaa gtatccctgc tgactgtcag ccttaatcca
    18781 ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg taaccaaagg tcaacgcttg
    18841 cagccaggcg gtgccgtcat aaagatcgtt gacgctgcga tcgtcaactt tatcgcgggt
    18901 accatagaac tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc
    18961 tttggcaaaa tattgatcga tatacccttc cgcctgacca aatgccgctt ccagtacgaa
    19021 gttatttttg aagtcgtatt tcgcaccgat ggagtgcaga taatcaactt tggtggtttt
    19081 atcgttctga taaaactcat ccatttccag atgccacggc gctttgtatt cgttggtcca
    19141 catgtaggag aaactcaagg caccagcatc gccgtaatca aaattcgccc cggcttccgc
    19201 cccctgataa gtacccggca taaagctcca gtgcggcgct aacagcgttt gaccggttgg
    19261 ctgaatgtaa cctgccctcg cccaaaccgg accgtattta aatttggccg cagctttata
    19321 caggcttata ccgcttttgt cgccggacca gtcttcgtca taggctttat tactttttga
    19381 aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc gccatttcaa tcgccgtaaa
    19441 cgcagcaata tcaaggccga acatatcagc agcataaccg gactgaaaat cgaggttggc
    19501 attccaggtg gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg
    19561 gtcgcgttca cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc
    19621 ttcggcgcga gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcaatagc
    19681 cagcgccagc gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta
    19741 taaattgctg caccaaaggt gaacagcgaa aaaaaataaa aattaaaaaa gtagcgttga
    19801 gaaaagtctc gctgactaca ggaacagact atttttcgcg acgcgaatta tcaatctctt
    19861 tctgtaacca gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa
    19921 ggcttaagtt tgcggttgaa aggcttgcag aagaataaaa acgtgaaatt ccagcaagat
    19981 gaaagcggtt acattttatt taattatgtt ttgtgatttt aaatttattt tatggataaa
    20041 ttgcgaagcg gaattaattc gcgaagaaaa gcagaaaaaa cgccgcagag cgcggcgttt
    20101 gggattgttt gctatttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc
    20161 acgcaaattc aatatattgc agagatcatg taggcctgat aagcgaagcg catcaggcaa
    20221 tttagcgttt gaaatttatt gtctatttat tgccggatgc gacgtaaatg cctcacccgg
    20281 cttggtagaa cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg
    20341 cggttaaata ccggtttttc ggccgtggaa tggcggctgt cgaggcagaa gtaaccttca
    20401 cgctcaaact ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc
    20461 tgtttgatca ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg
    20521 ttaggcacgc tgaacagacg atcatacaaa cggatttcaa ccggcagcgc atgtgccgcg
    20581 ctcacccagt gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg
    20641 tcggcgtcat aagtacagaa gatggtggtg atattacctt cggcatcttt ctccacgcgt
    20701 tcagccttaa tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg
    20761 tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatttc accgctaaac
    20821 ggcacctgac ggctccccat ttccggtttg ttcggatggt tcggcatggt gaccatttcg
    20881 ccttcgccct gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc
    20941 ggcgcatttt cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg
    21001 ttgtcctgct tggtcacgcc gatgcgtttg cagaactcac gaatagaagc cgcagtgtaa
    21061 ccacgacgac gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc
    21121 ttgtcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg
    21181 cgcgagaatt cgtactggcg cggatgaaca ggaatggtga tgttatccag tacccagtcg
    21241 tacagacgac ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc
    21301 gcatcgctga tgcaatgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc
    21361 tggtggtgtt cagcaaattt aatgcggtac agcaccggat cgcgcattac gataaatggc
    21421 gacgccatgt cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc
    21481 attttttcga acagcgccaa gttctcttca acgctgcggt cgcggtacgg gctgttttta
    21541 cccggctgcg tcagggtacc gcggtattcg cggatctgtt ccggtgtcag ttcatcaacg
    21601 tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg
    21661 gaggagtaac ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc
    21721 gaatcgacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac
    21781 tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata
    21841 tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtgatgtg cttaccactg
    21901 gccagatctt catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc
    21961 atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca
    22021 ggctgacaac tgttagtttt ttaaaattga taatctaaca aggattatgg ggatgattga
    22081 tgaaaaaaaa agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctgcc
    22141 gtgtcgcatc aggcataaag cagattactt tttgatttca tacagcggtg tttgacccgc
    22201 cacaacatgg ccctgagctt taatgagcaa gccactgaaa tcgtcgatat tgctgcaaac
    22261 caccgggcta atcatcgagc gggcgttagc gttcaggtaa tccagatcca tttccagaat
    22321 cggttgccct gcgcttacct gcgcaccctc ttccaccaga cgtttaaagc ctttaccttc
    22381 cagcgctacg gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag
    22441 gcagaacgcg tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac
    22501 gattttatct gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc
    22561 gtcaggaacc tgatccagtg ccacgacatc accggtaatc ggcgatacca gctccgcgat
    22621 agataccgcg tttggtacag cctgcggttt tgctacaggc gcggcagttg ccggagttgc
    22681 ttcagctgac gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat
    22741 ggattctgct ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt tcactacacc
    22801 agaagcaccc agacgtttac acatcgtatc gttgacgcgg gcagagtcag ccacggtcag
    22861 gcgcagacgg gtaatacagg catcaatcgc tttcaggttg tcagtaccgc caaccgcagc
    22921 aatatagttg gttgccagtt gagtcagacc ttcttcagta ttgctgttgg cttcttcagt
    22981 aacgatctcg tcttctttat cttcacgacc cggcgttttc aggttgaaca tgcggataac
    23041 caaactgaac accacgaagt agatagcgaa gaagataacg cccatcacca gcagcatcca
    23101 gacgttctgg ctggcggcag gcaggttata catcaacgcg tagtcgatag cacccgcaga
    23161 gaaagagaag ccggcatgga tacccagcag cgttgccaca aacaggctga taccggtcag
    23221 cagtgcgtgc aggaggtaca gcagcggcgc aaggaacatg aacaggaatt ccagcggctc
    23281 agtcacaccg gtcaggaacg cagtaacagc aacagaaagc agcatcccgc caaccatcgg
    23341 acgacgctct ttcggtgctg cgaagtacat cgccagcgcc gcacccggca gaccgaacat
    23401 catgatcggg aagaagccgg acatgaacat ccccgcggtg ccgtcaccag cgtagaaacg
    23461 gttgatgtcg ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa tctggaacca
    23521 ggcgatggtg ttcagcacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc
    23581 aaagataccg gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc
    23641 gtgctgtacc ggcggccaga cgtaaccaaa aatggccgcc agcaccagac agaagaagcc
    23701 ggtggcgatc ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat
    23761 atcggaccaa cggttatagg ctgcgccacc aaccagaccg gtaatgatac ccgccagtac
    23821 acccatgtta atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac
    23881 cgcacccgcc agcgccgccg cacctgcgct gtctttcgac cagctggatg ccacaccgat
    23941 ggcgaagatt aatgcgaggt tatcaaaaat tgcaccgccc gcctgggcaa taaacgcaac
    24001 gttaagtaaa tctggctgac cgaatcgcag caacagcgcc gccaccggca gcaccgcgat
    24061 agggagctgt aacgccctac cgagtcgctg gaaaaaacct aaaatattca tcttattccc
    24121 cctacgagaa ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattttac
    24181 tgccgatgtt ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat
    24241 tcgcatcgca aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc
    24301 actccctttt actggctaaa ccagaaaact tattttatca ttcaaaaaat caagtcggat
    24361 tgacgcctgt ctgcgcaaat ccaggttacg cttaaagatg tctaatccgc caacggctta
    24421 cattttactt attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg
    24481 gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc gttcaaacca actgccgatc
    24541 gtccgtttgt actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag
    24601 tcgaaatgca taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg
    24661 aatatgtcgg tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt
    24721 tcttcgatca cgttgatatt ccagcagaaa acatcaacct tctcaacggc aatgccccgg
    24781 atatcgatgc cgagtgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc
    24841 tgtttatggg cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc
    24901 tggcttctcg tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt
    24961 tctttgataa cgatgtcaat caggtgccaa aatatgccct gactgtcggt gttggtacac
    25021 tgctggatgc cgaagaagtg atgattatgg tgctgggtag ccagaaagca ctggcgctgc
    25081 aggccgccgt tgaaggttgc gtgaaccata tgtggaccat cagctgtctg caactgcatc
    25141 cgaaagcgat catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa
    25201 gatatttcaa tgaattagaa gcagaaaata tcaaaggtct gtaattgtta tccctgccct
    25261 ggttccttgc tcagggcaat attttttaaa atcggggggc agaatgtatg cattaaccca
    25321 gggccggatc tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg
    25381 cctgattaaa agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact
    25441 gaacggggcc attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt
    25501 acagtttaac gacaccgctg aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc
    25561 caatgagaaa tcaggctgta ctaactatct gccgacgctt atcaccacca gcgatgagct
    25621 gatgaaacag ggcgtgcgcg ttatgcgtga gtacctggca aaacatccga atcaggcgtt
    25681 aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa
    25741 ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat
    25801 taccaaagtg accctggcac cggaaatggt tcctgcggaa gtcatcagca aactggcaaa
    25861 tgccgggatt gtggtttctg ccggtcactc caacgcgacg ttgaaagaag caaaagccgg
    25921 tttccgcgcg gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg
    25981 tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat
    26041 cgctgatggc ctgcatgttg attacgccaa cattcgtaac gctaaacgcc ttaaaggcga
    26101 caaactgtgt ctggttaccg acgccaccgc gccagcaggt gccaacattg aacagttcat
    26161 ttttgcgggt aaaacaatat actaccgtaa cggactttgt gtggatgaga acggtacgtt
    26221 aagcggttca tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat
    26281 cgcactggat gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga ttgacgttga
    26341 aaaacgtctc ggcacgctcg ccgcaggtaa agtagccaac ctgaccgcat tcacacctga
    26401 ttttaaaatc accaagacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt
    26461 atgacaccag gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc
    26521 gcggcggttt atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag
    26581 caaagccagc ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg
    26641 ctgatcaaag aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc
    26701 accgaaaccc gcaatttcca cgcaatcggc gtacggcttg gtcgtcatga cgccaccatc
    26761 actctgtttg atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt
    26821 acccagcaga cgctggaaca tgccctgctg aatgccattg ctcagtttat tgatagctac
    26881 cagcgcaagc tgcgcgagct aatcgccatt tcggtgatcc tgccagggct tgttgacccg
    26941 gacagcggca aaattcatta catgccgcat attcaggtag aaaactgggg gctggtagaa
    27001 gcactggaag aacgttttaa agtgacctgt ttcgttggtc acgatatccg tagtctggcg
    27061 ctggcggagc actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc
    27121 catcgcggaa ccggggccgg gattatctct aacgggcgca tttttattgg ccgcaacggt
    27181 aacgtcggtg aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc
    27241 aactttggct gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat
    27301 ctgttaaagc agggctacca gagccgtgtg ccactggacg actgcaccat caaaactatc
    27361 tgcaaagccg cgaacaaagg cgatagcctg gcctcggaag tgattgagta tgtcggtcgt
    27421 catctgggta aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt
    27481 gctggtgaaa tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat
    27541 acccaagcgc tgaaggcgtt tcgtactaat ctgccggtgg tacgttctga gctggatcac
    27601 cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc
    27661 cagcatttgc tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg
    27721 ggtagtccat gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata
    27781 acgtcgccgt accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc
    27841 tggtgttgct gaccaactat ccttcgcaga ctgggcaaga tctggcgaac cgctttgcca
    27901 ctgccggtgt cgatgtgccg gatagcgtgt tttatacctc tgcgatggcg accgccgatt
    27961 tcctgcgtcg ctaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg
    28021 aactgtacaa agccggtttc actattaccg atgtgaaccc tgatttcgtg attgttggcg
    28081 aaacacgttc ctacaactgg gacatgattc ataaagcagc ctatttcgtc gctaacggtg
    28141 cacgctttat cgccaccaac ccggacaccc acgggcgcgg tttttatccc gcttgtggcg
    28201 cgttgtgtgc agggattgag aaaatctccg ggcgcaaacc gttctatgtt ggtaagccca
    28261 gcccgtggat catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacagtga
    28321 ttgtcggcga taacctgcgt accgatattc tggcgggctt ccaggccggt ctggagacga
    28381 ttctggtgct ttctggtgtt tcatcgctcg acgatatcga cagtatgccg ttccgcccaa
    28441 gctggattta cccgtcagtc gctgaaatcg acgttatctg aataccaccc acgtctgcgg
    28501 acgtggttgg ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt atcaggccta
    28561 cgcagctcct gcaatatatt gaattttcat gcttttgtag gccggataag gcgttcacgc
    28621 cgcatccggc atgaacaaat cgcactttat cagcaatttg gcccccgtaa aatataacca
    28681 ttcaataaaa cccgctaaaa attatcgatt catcaataaa tacatcctca caccagaccc
    28741 tttttcacca atcgacaatc accattacgt tttttatttt tgcactgcca aatagcttgc
    28801 gcacagtgct gctttgcggc atttttttaa acaagcaaac acaacaagca acaaatacca
    28861 ggttaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag
    28921 ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact
    28981 ggtccggtat ttatgccagc gataacgcca ttctcgctca cgaacgtctg tcaattgttg
    29041 acgttaacgc gggggcgcaa cctctctaca accaacaaaa aaaccacgta ctggcggtaa
    29101 acggtgaaat ctacaaccac caggcattgc gcgctgaata tggcgatcgt tatcagttcc
    29161 agacagggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc
    29221 tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc
    29281 tgattggtcg cgaccatctg gggatcatcc cactgtatat ggggtatgac gaacacggtc
    29341 agctgtatgt ggcctcagaa atgaaatcgc tggtgccagt ttgccgcacg attaaagagt
    29401 tcccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat tatcatcgcg
    29461 actggttcga ctttgatgca gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg
    29521 cactggaaga ttccgttaaa agccatctga tgtctgatgt gccttacggt gtgctacttt
    29581 ctggtggtct ggattcctca attatttccg ctatcaccaa gaaatacgca gcccgtcgcg
    29641 tggaagatca ggaacgctct gaagcctggt ggccgcagtt acactccttt gctgtaggtc
    29701 tgccgggttc accggatctt aaagcagccc aggaagtggc aaaccatctg ggcacggtgc
    29761 atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac gtgatttacc
    29821 acatcgaaac ttatgatgtg accactattc gcgcttcaac accgatgtat ttaatgtcgc
    29881 gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt
    29941 ttggcggtta tctttacttc cataaagcgc ccaacgttaa agaactgcat gaagagacgg
    30001 tgcgtaaact gctggccttg catatgtatg actgcgcgcg cgccaacaaa gcgatgtcag
    30061 cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc
    30121 gcattaaccc gcaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg
    30181 aatgttttga gtcatacctg cccgcaagcg tggcctggcg gcagaaagag cagttctccg
    30241 atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag caggtttctg
    30301 atcagcaact ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc tcaaaagaag
    30361 cgtatctgta ccgcgagatc tttgaagaac tgttcccact tccgagtgcc gctgagtgcg
    30421 tgccgggcgg tccttccgtc gcgtgttctt cagctaaagc gatcgaatgg gatgaagcgt
    30481 tcaaaaaaat ggacgatccg tctggtcgtg cggttggcgt tcaccagtcg gcgtataagt
    30541 aagaaatgcg gtactgcccc ggagaaatcc ggggctgttt tagttattgc gctgcttgtt
    30601 gattctgata tgcagtaagg aaatgttccg ggtttgccac atcactgttt gacaactcat
    30661 cactgtaggc cagtatttca tagattaatt tcccaccact attgttgaat tccgaatttc
    30721 atcctgtacg acaaaagaat ggctgtaggg tggggaaaaa cacaaagtaa acaataattg
    30781 acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga gcaattttcc
    30841 ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc aacgccgata
    30901 aggtatcgcg aaaaaaagat ggctacgtag ctcagttggt tagagcacat cactcataat
    30961 gatggggtca caggttcgaa tcccgtcgta gccaccatct tttttgcggg agtggcgaaa
    31021 ttggtagacg caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc
    31081 tcccgcacca ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc
    31141 ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc ttcttcgagt
    31201 aagcggttca ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata
    31261 ccggcattcc ctggttcgaa tccaggtacc ccagccatcg aagaaataat ctggctacgt
    31321 agctcagttg gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg
    31381 tagccaccaa attctgaatg tgtcgaatac gttcggcaaa ttcaaaaacc aatttgttgg
    31441 ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc
    31501 gtaccccagc caatttattc aagacgctta catcgtaagt gcacccagtt ggggtatcgc
    31561 caagcggtaa ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca
    31621 gccacattaa aaaagctcgc ttcggcgagc tttttgcttt tctgcgttca ttcaatgtcg
    31681 aatgcgatgt tgacacgtct tatccttcaa tgtcggatgc gacgctgccg cgtcttatcc
    31741 gacctacggc tggcacgcat ccggcaatgt tgtaaggcta caaccctaac gcatatttca
    31801 gcgcctgacg tttcaacacg ccagcacgct ctgccgccat taacccgaga ttacgcacaa
    31861 aacgcagcgg cggcagatta ttactgaatc cggcataaaa cagatccata ccactttgca
    31921 taatgaagtt atccgccatg cgccgcatct ggtaacgctt gaggacagga taactggccc
    31981 acgcttcacc gtagctgcgg gcattcacca gaacatcaat cagggcatcg acatcacgat
    32041 aaccaagatt caccccctgc cccgccagcg gatggatggt atgcgcggca tcgcccacca
    32101 gcgcaagccc cggctgcaca tactgcaacg catgtcggcg cgtcagcgga aacgcaccag
    32161 cggcaagcgg tgtaacgtaa cccagacgcg acgggaaatg cttcgcgatt tccgcctgga
    32221 gttgcgccat attcatattc tgcaactggc gaatacgcgt cggagagtca taccacacca
    32281 gcgatgccca gttatcaaac aacggcagaa acgcacgcgg tccgtccggg gtaaattgct
    32341 gccaggtgct gtcgccggga tcgttctcac actgtacgct aatcaacatg cacgactgcg
    32401 catactgcca ggcatgaacg ccaattcccg ccatctgccg cacctgagaa tttgcgccgt
    32461 cggcaccaat caccagtttc gcgcggatcg tttcaccgcc cttcagttcc agctcctgaa
    32521 gatcattatc tcgatgtaat gcaatcagcg aggttggcac acgtaacgtt actttcggat
    32581 gcgcttccag cgcctgccac aacgcctgtt gcaggactgt gttttccacc atgtagccaa
    32641 gcaacggcaa ctttaattcg gctgcgtcaa ataccacatg tgccgtttcc cactcccagg
    32701 tttccagtct gcggtaagga tggcaacgca tagcctgtac tgcatcccag acccctaacc
    32761 ctttaagcaa tgataccgaa gccgcgctga tcgccgagat ccgcacgtcc ggttggctgt
    32821 cagcgacaaa cggtgcaggt tctgcgtgct cgatcaccgt taccgaaaat ccgtgctgtg
    32881 ccagccccag cgccagtgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg
    32941 gttgatttgt catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta
    33001 cccccaaggg ctgataacgc tcatactggt cacaacggca gcaaagcatt acactatgcg
    33061 ccctgcattc ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta
    33121 aaacctgggg ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg
    33181 ccacccacgg ctatcaactg accgacgtgg cggaagaagc ggatgtgcta ctgctgaaca
    33241 cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac
    33301 tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg tggctgcgtg gcatcgcaag
    33361 aaggcgagca cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc
    33421 tgcaccgtct gccggagatg atcaactccg tgcgcggcga ccgcagcccg gttgtagata
    33481 tcagcttccc ggaaatcgag aagtttgacc gtctgccgga accgcgcgcc gaagggccga
    33541 ccgcgtttgt ctccatcatg gaaggctgca ataaatattg cacctactgc gtggtgcctt
    33601 acacccgtgg tgaagaggta agccgtccgt ctgacgatat tctgtttgag attgcccagc
    33661 ttgcggctca gggcgtgcgt gaagtcaacc tgctcggtca gaatgtgaat gcctggcgtg
    33721 gtgagaacta cgacggcacc accggatcgt ttgccgatct gttgcgtctg gttgctgcga
    33781 tcgacgggat cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata
    33841 tcatcgaagt gtaccgcgac acgcctgagc tggtgagctt cctgcatctg ccggtacaga
    33901 gcggttccga tcgcattctg aacctgatgg gacgtaccca tacggcgctg gagtacaaag
    33961 cgatcatccg taaactgcgt gcggcgcgtc cggatattca gatcagttct gacttcattg
    34021 ttggcttccc tggcgaaacc accgaagact tcgagaaaac gatgaagctg attgccgacg
    34081 tcaatttcga catgagctac agctttatct tctctgcacg tccaggaacg cctgccgccg
    34141 atatggttga tgatgttccg gaagaagaga agaagcagcg tctgtatatt ctgcaagaac
    34201 gtatcaatca gcaagcgatg gcatggagcc gtcggatgct cggcaccacc cagcgtattc
    34261 tggtagaagg gacttcgcgt aagagcatca tggagctttc cgggcgtacc gaaaataacc
    34321 gcgtggtcaa cttcgagggc acaccggata tgatcggtaa attcgtcgat gtagaaatta
    34381 ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg aactgaagat gaaatgggtc
    34441 tgcgcatggc agaaacaccg gagtcggtga ttgcccgcac ccgcaaagaa aacgaccttg
    34501 gcgtgggtta ttatcagccg taattctcag gcctgccgtt ccggtgggcc ttgttttttc
    34561 tctctttatc cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt
    34621 ccttataggc cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa
    34681 catagacact cgcgaaatca ccctggagcc agcagacaat gcgcgtctgt tgagcctgtg
    34741 cggcccgttt gatgacaaca tcaagcagct cgaacgccgt ctcggcatcg agatcaatcg
    34801 ccgcgataac cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat
    34861 tctgcgtagc ctgtatgtcg atactgcccc gatgcgcggt cagattcagg atatcgaacc
    34921 ggaacagatc caccttgcga ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt
    34981 gccggagtac ggcaaagcgg tcaatatcaa aactaaacgc ggcgtaatta agccgcgcac
    35041 gccaaaccag gcgcagtaca tcgccaatat tctcgaccat gacattacct tcggcgttgg
    35101 cccggcgggt acgggtaaaa cctacctggc agtggctgcg gcagttgatg ccctggagcg
    35161 tcaggaaatt cgccgtattc tgctgactcg tccggcggtc gaagccggtg agaaactggg
    35221 cttcctgcct ggcgatttaa gccagaaagt agacccgtat ctgcgcccac tgtacgacgc
    35281 gctgtttgaa atgctgggct ttgagaaagt cgagaaactg attgagcgca acgttattga
    35341 agtcgcgccg ctggcctata tgcgtggtcg tacgctgaac gacgcgttta tcattctcga
    35401 tgagagccag aacactacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa
    35461 ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac ctgccgcgta atactaaatc
    35521 aggcttacgt catgccattg aagtgctggc cgatgtcgaa gagatcagct ttaacttctt
    35581 ccacagcgaa gacgtggttc gtcacccggt ggtggcgcgt atcgttaacg cttatgaagc
    35641 ctgggaagaa gcagaacaaa aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga
    35701 acaggaacaa aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc
    35761 gggttaccgg aagagagcca gtttcaaaca tggctgaatg cggtgatccc gcagtttcag
    35821 gaagagtcgg aagtgactat tcgcgtggtc gataccgccg aaagccacag tctgaatctg
    35881 acctatcgcg gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtgccgcct
    35941 ggcatggaga tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa
    36001 gctcaggagc aaggcaaacc actggaggcg cactgggcgc atatggtggt gcacggcagt
    36061 ctgcatttgt taggttacga tcacattgaa gatgacgaag cagaagaaat ggaagccctc
    36121 gaaacagaga ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa
    36181 tttgccaggc atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga
    36241 ttaactaaca tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca
    36301 cgataagcaa caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac
    36361 cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg
    36421 acgaagatac gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg
    36481 acatcatgat cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat
    36541 gccttgatgt catcatcgag tccgcccact cacgtttccc ggtgattagc gaagacaaag
    36601 atcacattga agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg
    36661 aagccttcag catggacaaa gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg
    36721 tagaccggat gctgaaagag tttcgctctc agcgttacca catggcgatc gttattgacg
    36781 aattcggtgg ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg
    36841 agattgaaga cgagtatgac gaagaagatg atatcgactt ccgtcagctg agtcgtcata
    36901 cctggaccgt gcgcgcactg gcttccattg aagacttcaa cgaagcgttc ggcacccact
    36961 ttagcgatga agaagtcgac actatcggtg gtctggtgat gcaggcgttt gggcatcttc
    37021 cggcgcgtgg cgaaactatc gacatcgacg gttaccagtt caaagtggcg atggccgata
    37081 gtcggcgtat tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccaaagctgg
    37141 atgaataaaa ccgaaactgg atagataact acatggattt tgcctcatta attgaacgcc
    37201 agcgcattcg cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc
    37261 cttacgacgt ctggcctgcg gcgattattt cgctgatggg gcttcaggcg ttgaccttta
    37321 accgtcgccc actccagtct gccgctattg gcttttgctg gggatttggc ctctttggca
    37381 gcggtattaa ctgggtctat gtcagcatcg cgacctttgg cgggatgcct ggcccggtta
    37441 acatcttcct ggtggtgctg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg
    37501 gcgtgctgtc gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccacccctg
    37561 ctctctggca agtgaccgag tttctgcgcg gttgggtgct gactggcttc ccgtggttac
    37621 agttcggcta tagccagatt gatggcccgt taaaagggct ggcaccgcta atgggcgtgg
    37681 aagccattaa cttcctgctg atgatggtta gtggcctgct ggcactggcg ttggtcaaac
    37741 gcaactggcg tccgctggtg gtggccgtcg tgctgtttgc cctacccttc ccgctgcgtt
    37801 acatccaatg gtttaccccg caaccggaga aaaccattca ggtttcgatg gttcagggcg
    37861 atattccgca atcgctgaaa tgggacgaag gccagcttct taatacgcta aagatttact
    37921 acaacgcaac ggcaccgctg atgggcaaat catcgttgat tatctggccg gagtcggcga
    37981 taaccgatct ggaaattaat cagcaaccgt tcctcaaagc actggacggt gagttgcgtg
    38041 ataaaggtag ctcgctggta accgggattg tcgacgcgcg tctcaataag cagaaccgct
    38101 acgataccta caacaccatc atcacgctgg gtaaaggtgc accgtacagc tacgaatcag
    38161 ccgatcgcta taacaaaaac catctggtgc cgtttggcga gtttgtcccg ctggagtcga
    38221 ttctgcgtcc gttagcaccg ttctttgatc tgccgatgtc gtcgttcagc cgtgggccat
    38281 atatccagcc gccgctgtcg gcaaatggta ttgagcttac tgcggctatt tgctacgaga
    38341 ttattctcgc cgagcaagtg cgcgataact tccgcccgga taccgactac ctgctgacta
    38401 tctccaacga tgcgtggttt ggtaaatcta ttggtccatg gcaacacttc cagatggcgc
    38461 gaatgcgtgc gctggagctg gcgcgcccac tgttgcgcag caccaacaac ggcattacgg
    38521 cggtgattgg cccgcagggt gagattcagg cgatgatccc gcagttcgcc cgcgaggtgt
    38581 taaccactaa cgtgacgccg accaccggac tcacaccata cgcacgtacc ggcaactggc
    38641 cgctgtgggt gctgacggca ctgtttggtt ttgctgctgt gttgatgagt ctgcgtcagc
    38701 gacgtaaata atcccttctt atcgtgccgg aatacatccc atatttcggc acgctcaccg
    38761 gatgcggcat cagtgcatta tccggataac aatattctcc tatccaactt tggcacatct
    38821 attgctttgt tatacaaggc aaagcttgaa ccagcatcag tacaacccgg ttgcaccaca
    38881 gcaatccacc ggtagcacca aaagagtgca ttttaagaat tttgcttcag aatggtgcgg
    38941 tgcacctcgc aaaaacaaac aataaaacat caatatcttt acatttagcc aaactaaatg
    39001 ttacctaaca ggcacaacac tgcacaataa agttgcagac gataacaaca caaacactca
    39061 caacgggtat ccatgcgttc ttaacgcaga agataaaggt gttggatatg caattacgta
    39121 aacctgccac agcaatcctc gccctggcgc tttccgcagg actggcacag gcagatgacg
    39181 ccgccccggc agcaggcagt acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg
    39241 gtcaccgtga atcttcagta cctttctctt attacgacaa tcagcaaaaa gtggtgggtt
    39301 actcgcagga ttactccaac gccattgttg aagcagtgaa aaagaaactc aacaaaccgg
    39361 acttgcaggt aaaactgatt ccgattacct cacaaaatcg tattccactg ctgcaaaacg
    39421 gcactttcga ttttgaatgt ggttctacca ccaacaacgt cgaacgccaa aaacaggcgg
    39481 ctttctctga cactattttc gtggtcggta cgcgcctgtt gaccaaaaag ggcggcgata
    39541 tcaaagattt tgccgacctg aaaggcaaag ccgtagtcgt cacttccggc actacctctg
    39601 aagttttgct caacaaactg aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca
    39661 aagatcacgg tgactctttc cgcaccctgg aaagcggtcg tgccgttgcc tttatgatgg
    39721 atgacgctct gctggccggt gaacgtgcga aagcgaagaa accagacaac tgggatatcg
    39781 tcggcaagcc gcagtctcag gaagcctacg gttgcatgtt gcgtaaagac gatccgcagt
    39841 tcaaaaagct gatggatgac actatcgctc aggtgcagac ctccggtgaa gcggaaaaat
    39901 ggtttgataa atggttcaaa aatccaattc cgccgaaaaa cctgaacatg aatttcgaac
    39961 tgtcagacga aatgaaagca ctgttcaaag aaccgaatga caaggcactg aactaattac
    40021 aagaaccagg ggcggaaatt ccagccctct cgattgttac gtagcacgga cagactatac
    40081 gcctgatggt cgttccccat cgggcctgaa aaccgcaata cgctgggtaa caatcttcga
    40141 gggtagcagt taacgctgct accctctttt ttctggagta gatttatgtc tatagactgg
    40201 aactggggta tttttttaca acaagccccg ttcggcaata ccacctatct tggttgggtc
    40261 tggagtggtt ttcaggtgac gatcgctttg tcgatctgcg cctggattat cgctttcctc
    40321 gtcggttcat ttttcggcat tttacgtacc gttccaaacc gttttctctc cggtctgggt
    40381 acgttgtatg tcgaactgtt ccgcaacgtg ccgttgattg tgcaattctt tacctggtat
    40441 ctggtgatcc cggagctgct gccggagaaa atcggcatgt ggtttaaggc cgagctggat
    40501 cccaatattc agtttttcct ttcatccatg ctctgcctgg ggctgtttac cgccgcccgt
    40561 gtttgcgaac aggtacgcgc cgctattcag tcgctaccgc gtgggcagaa gaatgccgcg
    40621 ctggcgatgg gcctgacgct accgcagact tatcgatacg tactgctgcc taatgcttat
    40681 cgcgttatcg tcccgccgat gacttccgag atgatgaacc tggtgaaaaa ctccgccatc
    40741 gcctcgacca tcggtctggt ggatatggcg gcgcaggcgg gtaaattgct cgactactcg
    40801 gctcacgcct gggagtcgtt taccgccatc acgcttgcgt atgtgttgat taacgctttt
    40861 atcatgctgg tgatgacgct ggttgaacgt aaagttcgcc tgcccggcaa catggggggc
    40921 aaataatgta cgagtttgac tggagttcca ttgttccttc cctgccatat ctgctcgacg
    40981 ggctggtgat caccctgaaa atcaccgtca cggcagtcgt gattggtatt ttgtggggta
    41041 ccatgctggc ggtgatgcgc ttgtccagct tcgcgcctgt ttcgtggttt gccaaagcct
    41101 acgttaacgt attccgctcg attcctttag tcatggtttt gctgtggttt tacttgattg
    41161 tgccgggatt tctgcaaaat gtgctgggat tatcgccaaa aaatgatatt cgtttaattt
    41221 cggcgatggt ggcgttttcg atgtttgaag cggcctacta ttcagagatt atccgtgccg
    41281 gtattcaaag tatttcccgt ggtcagtcga gcgccgcgct ggcgttgggg atgactcact
    41341 ggcagtcgat gaaactgatt attctgccgc aggctttccg cgcgatggtg ccgctgctgc
    41401 tcactcaggg catcgtactg ttccaggata cctcgctggt gtatgtgtta tctctggccg
    41461 atttcttccg taccgcctca accattggtg agcgtgatgg tacgcaggtc gagatgatcc
    41521 tgtttgccgg atttgtttat ttcgttatta gccttagcgc gtcgttgttg gtcagctact
    41581 tgaaaagaag gacagcataa tgattaccct gaaaaatgtt tcaaaatggt atggtcactt
    41641 tcaggtgctg accgactgct caaccgaagt gaaaaaaggc gaagtggtgg tggtttgcgg
    41701 cccgtctggt tccggcaaat caacgctgat taaaaccgtc aacggcctcg aaccagtgca
    41761 gcaaggtgaa atcaccgtcg atggtatcgt ggttaacgac aagaaaaccg atctggcaaa
    41821 gctgcgctcc cgcgtcggga tggtattcca gcatttcgag ctgttccctc atctgtcgat
    41881 tatcgaaaac ctgaccctgg cgcaggtgaa agtgcttaaa cgcgataaag cgcccgctcg
    41941 tgaaaagcat tgaaattgct ggagcgcgtg ggactttccg cacatgccaa taaatttccg
    42001 gcgcagcttt ccggcggtca gcagcagcgc gtggcaatcg ctcgcgcatt gtgtatggct
    42061 cctattgcga tgctgttcga cgaaccgaca tcggcgctgg atccggagat gatcaacgaa
    42121 gtgctggacg tgatggtgga actggcgaac gaaggaatga ccatgatggt ggtgacccac
    42181 gaaatgggct ttgcccgcaa agtggcgaat cgggtgatct ttatggacga gggtaaaatt
    42241 gtcgaagact cgccgaaaga cgctttcttc gatgatccga aatcggaccg cgcaaaagac
    42301 ttcctcgcga aaatcctgca ttaatcactc tggcgcacat acccaattgc gcgccatctc
    42361 acgctttatc gtttcgcccc tcgtatctgt tacatcctgg cgttaccctt gtcgcaaaga
    42421 agcacacaac aaggagcaac aatggcactg ccaattctgt tagattgcga cccaggtcat
    42481 gatgacgcta tcgcaatagt tctcgcactc gcctcaccag agcttgatgt caaagcaatt
    42541 acgtcttccg ccggaaacca gacaccagaa aaaaccttac gcaatgttct gcgtatgctg
    42601 accttgctta atcgcaccga tattccggtg gcaggcggcg cggtaaaacc gttaatgcgt
    42661 gagttgatta tcgcggacaa tgtgcacggc gaaagcggtc tcgacggccc ggcattaccg
    42721 gaaccgacgt tcgcaccgca aaactgtacg gcggtggagc tgatggcgaa aacgctgcgc
    42781 gaaagtgcgg aacctgtcac gattgtttct accggaccgc aaactaacgt tgccttgctg
    42841 ctcaatagcc atccggaact gcatagcaaa attgcccgta tcgtgattat gggcggcgca
    42901 atggggcttg gtaactggac gcctgcggct gaatttaata tttacgttga cccggaagcg
    42961 gcagaaattg tcttccagtc agggatcccg gtggtgatgg ccggtctgga tgttactcat
    43021 aaagcgcaaa tccacgttga agacaccgag cgtttccgcg cgattggcaa ccctgtttca
    43081 accattgttg ccgaactgct ggatttcttc ctcgaatatc ataaagacga aaaatggggc
    43141 tttgtcggcg caccgctgca tgacccatgc accatcgcct ggctgttgaa acccgagttg
    43201 tttaccactg ttgagcgctg gcttggcgtg gaaacacagg ggaaatatac ccagggtatg
    43261 acggttgttg attattatta tctgactggc aataaaccga atgccactgt aattgtcgat
    43321 gttgatcgtc agggctttgt tgatttactg gctgatcgtc tgaaatttta cgcttaacac
    43381 tcgtcattga ttaagatcac cacgcgttac ctgggtggtc ttaatctatt tatctctttt
    43441 tctttcaaga tataaatgca atggataatg cagaactcgc cataggtatc gatctcggca
    43501 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    43561 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    43621 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    43681 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    43741 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    43801 ttaaagatgt ggtgatctcc gttctggctt atttcagcga tgaacaacgc aagcataccc
    43861 gtttagcagc ggacttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    43921 ctgcgatggc gtatggcctg catacccaac aaaatacccg ttcgctggtg tttgatctcg
    43981 gtggcggcac gtttgacgtt acggtgcttg agtacgccac gccggtgatt gaagttcacg
    44041 cctccgctgg cgacaacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    44101 ttttaaagcg cgcggatgtc acaaggacca cgcttaacga gagtgaactg gcagccttgt
    44161 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc aatgcacatt cgctggtagt
    44221 accaggatga aacgcgggaa tgcgaatttt acgagaacga actggaagat ttgtggttgc
    44281 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgcg cgtctgaagc
    44341 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    44401 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgatccg agcaccattg
    44461 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagaag
    44521 tgatcctcac tgatatttgc ccttactcgt tgggcgttga ggttaaccgc cagggcgttt
    44581 ccggcatttt ctcgccgatt attgaacgaa acaccactgt gcccgtgtcg cgtgtagaaa
    44641 cttattcaac catgcacccg gaacaggatt caattacggt taacgtctat cagggagaaa
    44701 accacaaagt taaaaacaac attctggtgg aatccttcga tgtgccgttg aagaaaaccg
    44761 gggcttatca gtcgattgat attcgcttta gttatgatat caacgggttg cttgaagttg
    44821 acgtgcttct ggaagacggc agcgttaagt ccagagtgat taaccacagc ccggtaacat
    44881 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    44941 gcgatatgct catcaatcgc acctttaaag ccaaactgga agagttgtgg gcgcgggcgc
    45001 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgctgcagt
    45061 caaacgatat ggcccgcgtc gatgaggtca ggcggcgggc gagcgtttat ttagccattg
    45121 agacctcata atcacggctg ccggatgcgg cgtaaatgct gcatccggca tcggcgcaaa
    45181 aaacacatcg tcaactaaac aacatcgcga ttttgccgag agcataacct ataaccagca
    45241 ccgttaaact gattttgata atgctggtaa tgctctgtgg tttttcaccc tcatcatttt
    45301 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttta
    45361 ctttcgtcaa cagcatccgg gtgataatgg cctctaatgg atgagcatct ccgccttcca
    45421 gccttgtatt aagacgggcc agcatctctt gtttcggaaa cgcttcaagt actacctgtt
    45481 ccgctacccg atacaaatcc agtaacggtg aatcttcttc tgtctcgcta ccgactctcg
    45541 atttccacca cgcccatgct ttgcgtttga agaataacga ctcaataaac gattgaccta
    45601 acggcgataa ttttccgcta tgtaaaattt ccgcccataa cagccgcgtc cgggcgctct
    45661 caccaggaac atatttcagc ggttgacctt gcgcatctac catgctccgc gcttcgataa
    45721 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    45781 tatcagcttg tgtagatggg taactttccc agtacgcgcc gagatcagca agcaggcttt
    45841 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    45901 gttcttccgc ttgccactgt ttggctacag gaatgagttc caccatacct gtttgcaccg
    45961 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    46021 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    46081 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    46141 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca
    46201 ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat
    46261 ttaatgacag atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga
    46321 catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg gagggctgaa
    46381 aacgatcact ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg
    46441 tggcatcttt ttcttcatca tctgcttcct cgacaggatt taccgccagt cgcagcgcct
    46501 cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacacaacg
    46561 gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga
    46621 ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg
    46681 ctggacagtt aatggctgcc agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag
    46741 ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag
    46801 cgaagcgttg tcttcgcact gagaagattg ccagtagaga cgccctagtg aataggcaga
    46861 aaaatagtgc atccagctgt gatagtaatg gtgagctcgt gcatagacgc gcgattgcag
    46921 ccataaactc tcttcttccg tcagccagtt gttgagcaca ccggcccgtg acagaaatcc
    46981 cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc
    47041 aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg ctttccactc
    47101 ttcaggggaa gagcgttgcc atgtcagata taagccagct aaatcattgg catgaccatc
    47161 atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga
    47221 tgagagatcg aattctttta tcccggtata catttttggc gagtcatgcg cgggtttgca
    47281 actctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt
    47341 attcattttt taataatccc ttaagtgcat taactgacta cttttttatt attgatgagt
    47401 ataaacttga cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg aataaaaaat
    47461 taatgtatat attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga
    47521 aaacgatgtt gcgtgataaa gccatggtga attatgcctt tgattattta agatcaccag
    47581 gcagtctacc gttaaccacg gcagccacgg agctttccgc gattcatggt cactcaacat
    47641 cgcaatatcg ccttggcgaa ttttatcttc atggtagcga cggtaaacca ctggattata
    47701 cacaggcgag atactggtat gagcaatcag cagaacagga aaatccgcgc gcacaaagca
    47761 aactggggtg gatctacctg aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc
    47821 tctggtataa ggaagcagct gaacaagggt atgctcatgc tcaatatact ttgggtttga
    47881 tctacagaaa tggcactggt attaatgtta accattatga atctcaaaaa tggttaaaac
    47941 tggccgccaa acaacattac aaaaatgcgg aaagattact tgccggactt cccgcacatt
    48001 aacgtcgact gtcacccgaa gagatggagt attttcccta tcaaaccggc aaagataaaa
    48061 atgtagaaaa taattttgat gacgcccaga cagcccagcc cgccatcatc aacgggcatt
    48121 ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt
    48181 aataatcgac gaataattaa tgcttccagt ggacaacgat cttctgcatc aaggcggctc
    48241 acaatcggca ttaatgattc ttctgtcggt tggctggcct gcactaattg tttggcaact
    48301 aaatataatg gccataaggg atgttctgta tcacgcgtga gtgagatccg cggcttacgt
    48361 cctttgtgca aaaccatctc gaccagtgaa cgccccagcg cagagagttt ttcatcgctg
    48421 agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata aagtagcggt
    48481 tttccctgat cgttaaccag atcacgcgct tcaatcatca tcaccactaa tgggtaataa
    48541 tagacccgat gctgacggca ccattgcaac attaaagcat ccgcctgggt ggagggatac
    48601 tcgcgccagt agtaacaaag ttctgccagc aggctttcgc cttctccaca atagacgcgt
    48661 tgtgcgtatt cataataacg cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg
    48721 gcgtaatcca ggagttcggg gattcccagg cggcttgcgc tataccagcg gagtaaattt
    48781 ctgtgcagtt ttgcatcatc ggggataatc actgcgcgat gttgcgctaa atacaatgtc
    48841 agccattctg gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca
    48901 ttttgcacgg caactggcag atgcagaatg gtctggaagt taaaaacatt ccccttcgca
    48961 atggcatata aaaaggattc cagttcttct tcgtctattt ccccgacgtt ttcctcctgc
    49021 aaccaccgca atctttctgc taataacacc acgcactcga aggataaatg ggcagtgttc
    49081 atggcgattg tgcacagcga ccagcgtaat tcatcaatct cttccatcga gcaataattt
    49141 aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac gttcgctatc agaggcaagt
    49201 aacgcgcgaa aggcgagcag aatttcatgt tctgctacct catattcttc tggttgccaa
    49261 acagatttag caggcgactg cgcaatccgt agcgcctcct catacgcctg acgaagttgt
    49321 ttaaaaccct gcggatcggt ttctggatga aaggacggta acagcgccag ataagcgcgg
    49381 cggataatat cgacgtcagt cgtttcctct atatcgagga tcttccagca atttttcata
    49441 agtcgctcat atccttaagc gtttccgggc atgtggggta gtcagccagt ggttgccagg
    49501 acaaggtagg gtaatacgca tcatcactgg caaataattg ataaaacata tcattgccgg
    49561 aatcgtcgta ttctttacgc gctaagaatt cgcgtaataa aggcagatga tcatcttcgg
    49621 gagactgcca atattgccga ccaaaggtat aaccggcaaa gtattgccgc cagttactat
    49681 aataacgtag agcccgtaga tgaatacgcg actggatcca tagactttct tcttcactca
    49741 accagttatt aagtacgccc attcgactta aaaaacccat ccggacataa tcccatgctt
    49801 taattcctcc ctcaccacag cattcggctg tgctggcgac aaattgcgca taagcctgac
    49861 cttgctcatt taattcagca agatagtcac gccattcaca tggagaataa cgaaaccagc
    49921 ggtgataaaa ccctgccaac cgagcggcat gaccgttctc gatcattcgg tgaattatgc
    49981 gcagcaggtc atcacggtca tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac
    50041 ctaaatacat tttgggggat gaatgcgaag gtaaacagca ttcattgcgt atttccatcg
    50101 gagtggaaag tgcaaacaat attttttgcg accccatgtc catataattt atccttaaac
    50161 attcatttaa tgaatgtaaa ctacatttcg aggctcagtt atttatactt ttctgacgca
    50221 acataatacg cataaaataa ggcttacgca aaaatggaac acataaaaat ataatatatt
    50281 ttatctacaa ccataaggaa atgataatga tttttacgtc aagttgctgc gataatttat
    50341 caatagatga gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg
    50401 ggttttatta taatcgcgat agcgcaattg actctccgga cgacgaaaag gccttttact
    50461 ggctgaagct ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt
    50521 ataccgagga taaaagccgt cataaagata atgagcaagc catcttctgg ctgaaaaaag
    50581 ctgccctaca aggccatact ttcgcttcca acgcccttgg ctggatactg gatcgtggag
    50641 aagaccccaa ctataaagaa gcggttacct ggtatcagat agccgcggag agcggaatgt
    50701 cttatgcgca aaataatctt ggatggatgt acagaaatgg caacggagtc gcacaagact
    50761 atacgctggc atttttctgg tacaaacaag ctgcattaca aggccatagt tacgcacaag
    50821 acaatctggc cgatctttat aaagacggag aaggcgttgc tcaaaacaag acactcgccg
    50881 cattctggta tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat
    50941 gggattataa cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc
    51001 tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac
    51061 acggacaagg cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg
    51121 aatgcaatga cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa
    51181 gacctgtcga tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca
    51241 gagatgtcag tcaggaaatt cgtgagactg aagctttact gtagaaataa aaacaagggc
    51301 cggggaattt cattctgccc atattctctt tattgctgtg cgaaatcaga ctattacggc
    51361 tcaaacgggc gtctctggaa ctggtcataa ccacatttcg ggcatagcgt cagcacttcc
    51421 ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc
    51481 acttctccgc tgtgataaac cccatgatga ttgaggtcct ggaaaatttc gcgccattca
    51541 agctgcgttt tatcggtgat gtctgccagc tcctgccaca agctttcttt aatcacccgc
    51601 ataaagacgc tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc
    51661 aggtcacgtc tgacagctcg cgtcagctca tcgatctcgg ttcgcgttaa ctcccctgtt
    51721 tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc
    51781 aggcgttcgt tcagtgacgc gaccagttca cggtaatatt gagcaacctt gttcatcgtt
    51841 tcatctcccg ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta
    51901 atacgcccac aaactctgta aattaatgta cgaataatcc gggcatcatt tcaacgtcga
    51961 gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc
    52021 acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc
    52081 aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat
    52141 ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc
    52201 cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc
    52261 gttaccagcg catgctgggc aaaaacgttc tgcagccgat cggctgggac gcgtttggtc
    52321 tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca
    52381 acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg
    52441 agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt
    52501 ataaaaaagg cctggtatat aaaaagactt ctgcggtcaa ctggtgcccg aacgaccaga
    52561 ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg
    52621 aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca
    52681 acgatctgga taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga
    52741 tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacacgctga
    52801 ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg
    52861 gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg
    52921 aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg
    52981 atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa
    53041 acttcgtatt gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc
    53101 gcgactacga gtttgcctct aaatacggcc tgaacatcaa accggttatc ctggcggctg
    53161 acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact
    53221 ctggcgagtt caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga
    53281 ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc
    53341 gccagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc
    53401 caaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta
    53461 ccagcccgat taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc
    53521 tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca
    53581 cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg
    53641 tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct
    53701 tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc
    53761 tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta
    53821 actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag
    53881 cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga
    53941 agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc
    54001 tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg
    54061 aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt gtacgagcac acagcaaaag
    54121 gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg
    54181 atgtgcataa aactatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca
    54241 ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcaccg accgatggcg
    54301 agcaggatcg cgctctgatg caggaagcac tgcaggccgt tgtccgtatg ctcaacccgt
    54361 tcaccccgca catctgcttc acgctgtggc aggaactcaa aggcgaaggc gatatcgaca
    54421 acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg
    54481 tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgtccc ggtggacgca acggaagaac
    54541 aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttactg
    54601 tacgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg
    54661 aggaagcgtg cgatatctgg caacactgtt gttatctctg gcggtgttaa tcaccgccgg
    54721 gtgtggctgg catctgcgtg acaccacgca ggttccttcc actatgaagg tcatgatcct
    54781 ggactcaggc gatccgaacg ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa
    54841 tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg
    54901 taaagtgagc atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta
    54961 tcagatgatc atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat
    55021 tagcgccaaa gtcttccgtt cattcttcga taacccgcaa atggcgttag cgaaagataa
    55081 cgaacaagac atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa
    55141 gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac
    55201 ggatactccg gcaacgcctg cacgcgtctc caccatgctg ggtaactgat gattcggttg
    55261 tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt
    55321 ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgccaggt agctgcggca
    55381 caaggattcg aagaacacca cactttttcc attgatccca acactgactg gaatgcgatc
    55441 ttttcgttat gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta
    55501 ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg
    55561 catgacgacc tgctattgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc
    55621 gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag
    55681 gctcagcttc cccgctgggt tgctgtgcgc gcaaaacagc tcaacttaga actggatgac
    55741 gcggcgaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca
    55801 ctggagcgtt tatcgctgct ctggccagac ggcaaactga cattaccgcg cgttgaacag
    55861 gcggtgaatg atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga
    55921 aaaagtaagc gcgcgttgca tattcttcag caactgcgtc tggaaggcag cgagccggtt
    55981 attttgttgc gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct
    56041 gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc
    56101 atgatgggcg aggcgttaaa tcgcttaagt cagccgcagt tacgtcaggc cgtgcaactc
    56161 ctgacacgaa cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg
    56221 gaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga
    56281 tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct
    56341 aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa
    56401 taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct
    56461 tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa
    56521 tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag gaccggacgt
    56581 gccgctggcg tttattattg gtcaggattc attgctgacc tttccgacct ggtacgaata
    56641 cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga
    56701 aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga
    56761 tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc
    56821 ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga
    56881 accggtattg acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag
    56941 ggcatgatgc gactgtggtt aattcgtcat ggcgaaacac aagcgaatgt cgatggtctg
    57001 tacagcggtc atgcgcccac acccctgacc gcacgcggta ttgagcaagc gcaaaatctg
    57061 catacactgc tagatgatgt ttcctttgac ttggttttat gcagtgaact ggaacgggca
    57121 cagcataccg cgcgactggt tctcagtgac cgccagctcc ccgttcatat catccctgaa
    57181 ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa
    57241 gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgctatccc cacgaacggt
    57301 gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct tagtgaatat
    57361 cagcactatc agaatatttt aatcgtcagc catcagggcg tactgagtct gttaattgct
    57421 cgtttaattg gcatgcctgc cgagtctatg tggcattttc gcgttgacca ggggtgttgg
    57481 agcgccattg atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc
    57541 ggggtcgaga acgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca
    57601 gccccgggca ggctgatatt ctccgcagcc agactttttc cgccagacac gactttgtag
    57661 aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt
    57721 tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag
    57781 accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga
    57841 ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca
    57901 aggcaaatcc agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt
    57961 tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg
    58021 cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca
    58081 tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt
    58141 gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac
    58201 cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg
    58261 caaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt
    58321 ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac
    58381 gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg taagtctact
    58441 gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggctgagc agagctggtc
    58501 gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta
    58561 ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag ggagctttga
    58621 gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc
    58681 gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct
    58741 tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga cccgctctaa
    58801 tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa
    58861 cggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc cggagaaagt
    58921 cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gtagatctga ccgatgacga
    58981 tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa
    59041 aactaaccta accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg
    59101 tgtcgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt
    59161 catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa
    59221 actggccaac tatgcggcaa cgcatgatat tggtaagctg ggcattgagc gttactatga
    59281 agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt
    59341 tatccgccag ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga
    59401 tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt
    59461 taccgaaccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt atgacccaaa
    59521 cctgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgctgaacg acccgaatac
    59581 accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag ttaaacccta
    59641 tgtggcggtt tcggcattaa gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc
    59701 aggctggtgg caactaccag ggtcggaaaa acgttatcgt gactggaaaa aatggggcca
    59761 cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt
    59821 ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca
    59881 ttacaccggt atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa
    59941 acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggcca
    60001 gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga
    60061 cggtatcgtg aaggttccgc atttgctgat gagcaccgcc gaagacggca aacaggtgcc
    60121 atgggtacaa ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc
    60181 gaaagacggt atgtacggtg ttgctaaccg ccctaatggt acggcgaata aatactttgc
    60241 cagcgcaccg tacaaaattg cggcgaaatc tggtaccgct caggtcttcg gtctgaaagc
    60301 gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac
    60361 cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggcgg
    60421 cgcgggtccg gcggttggta cgctgatgcg ccagatcctc gaccacatta tgctgggtga
    60481 taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc
    60541 atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg
    60601 ctgctgattt tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt
    60661 caggatattg gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg
    60721 gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc
    60781 atctgtatta ttttgctggt ggcggtagat gctttcggag ccatctctaa aggcgctcaa
    60841 cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta
    60901 ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaatact
    60961 ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg
    61021 ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc
    61081 tggcgtctga ttggcgtcgc agtagtgctg gtagcggcgt tcattccgat tctgtggttc
    61141 ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac
    61201 ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta
    61261 cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc cgaacgccat
    61321 actgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg
    61381 ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc
    61441 acctttggtc gcgtcatggc tggtggctta atgctgatat tattcgttta tgtcttcgta
    61501 aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt
    61561 tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac
    61621 acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct
    61681 cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca
    61741 gacggtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc
    61801 ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa
    61861 aagctacaaa atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta
    61921 tgatgccgaa ccaggcagta acctgacggc ctctggcgaa gctttcgatc cgacaaagct
    61981 gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa
    62041 cgggcgaatg atcgtggtgc gcattaatga tcgcggccct tacggcaacg accgcgttat
    62101 ttcgctttct cgcgcggcag ctgaccgtct taacacgtca aacaacacca aagttcgtat
    62161 cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac
    62221 agtcgccaaa cagacttacg tcctgcctgc acctcccgat ttaagcggtg gcgcgggaac
    62281 aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaattcga cgctaaaaag
    62341 cgaagatccg accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt
    62401 agcgcctggt gtactggaag gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc
    62461 ttcgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag
    62521 cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca
    62581 gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc
    62641 ggtctggcgg atccagcttg gcccattcgc cagcaaagcc gaagccagta ccttgcagca
    62701 acgtttgcaa accgaagccc aattacagtc atttattact accgcgcagt agcgtaaagc
    62761 aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat
    62821 gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt
    62881 agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac
    62941 ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat
    63001 gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg
    63061 caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat
    63121 gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt
    63181 agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
    63241 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct
    63301 gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc
    63361 ttttgttggc ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata cccacttcca
    63421 gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat
    63481 cggccaggca ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt
    63541 tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa
    63601 tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaatcttg ttgcttctgc
    63661 gactgaaggc cagatgcgct tgatttctgc ggtgatgggt ggacgtactt ttaaaggccg
    63721 tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc
    63781 actgaaagta ggtaaagagt tcgcctctga accggtttgg tttagtgatt ctgatcgcgc
    63841 ttcgttaggg gttgataaag acgtgtacct gaccattccg cgtggtcgca tgaaagatct
    63901 gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt
    63961 cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt
    64021 gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
    64081 gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat
    64141 actaagcatc agtaaaaaaa ctcccgcctt ctggcgggag ttgctattta attacgttac
    64201 gccggagctg acatgaaaac caaacttaac gaactgcttg aattccctac tccttttact
    64261 tacaaagtta tggggcaggc gttacctgag ctggttgatc aggtggttga agtggtacag
    64321 cgccatgcgc caggtgacta caccccaacg gtaaaaccaa gcagcaaagg caactaccac
    64381 tcggtatcta tcactatcaa cgccactcat atcgagcagg ttgaaacact gtatgaagaa
    64441 ctgggcaaaa tcgatattgt ccgcatggtt ctgtaactcg cttctccgtt acccggctcc
    64501 tggtcgggta actcccctct cctcgctgtg atatactttt cccccacttt tactcattct
    64561 ccacggagat gccgttttgt atcaggataa aattcttgtc cgccagctcg gtcttcagcc
    64621 ttacgagcca atctcccggg ctatgcatga attcaccgat acccgcgata atagtaccct
    64681 tgatgaaatc tggctggtcg agcactatcc ggtattcacc caaggtcagg caggaaaagc
    64741 ggagcacatt ttaatgccgg gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt
    64801 gacttatcac gggccgggac aacaggtgat gtatgtgttg cttaacctga aacgccgtaa
    64861 actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca gtggtgaata ccctggctga
    64921 actgggtata gaagcgcatc ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa
    64981 aatttgctca ctgggtttac gaattcgacg cggttgttca ttccacggtc tggcattaaa
    65041 cgtcaatatg gatctttcac catttttacg tattaatcct tgtgggtatg ccggaatgga
    65101 aatggctaaa atatcacaat ggaaacccga agcgacgact aataatattg ctccacgttt
    65161 actggaaaat attttagcgc tactaaacaa tccggacttc gaatatatta ccgcttaatt
    65221 ccatacatca atggcccaat ttacactggg tcattactca taatacctgc taatgcttta
    65281 catctccttc gcgtttgtat attttcttta tcacttgcag aaatgcggtg ccacgccttg
    65341 ctccgtaact caccccattg attaacctat tgaaatagtt gattatggtg agcaaaagcg
    65401 ttccgttcca attagacata agattattgt aattttttaa atattatttt tccatgaata
    65461 aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc
    65521 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac
    65581 tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct
    65641 gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt
    65701 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta
    65761 tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
    65821 gcaacgaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat
    65881 ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga
    65941 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac
    66001 caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcaa
    66061 gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt
    66121 acttctgtta ccggaagggc aaaatttcag cggtctgcgg cagagagttc aagagatgtt
    66181 tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc
    66241 caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta gtcgttgctg
    66301 gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca
    66361 ctacaacaaa ttcatcctgc gtgatcccat cctgcacgga gtaatagatg ttatccgtaa
    66421 tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa
    66481 tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga
    66541 tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa
    66601 cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg
    66661 aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg
    66721 caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg
    66781 actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct
    66841 gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta
    66901 tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc
    66961 cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc
    67021 tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccaac
    67081 actttgcgga ttgtattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc
    67141 tggtgccgga tttccgcggt cgaatggatc gtgctctgga tattctgact gcaacgcccc
    67201 cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccggcag gtacggcctg
    67261 gcgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa
    67321 tcccgaccaa gtctggtctg atggtgggtc tgggtgaaac caatgaagaa attattgagg
    67381 taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc
    67441 caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga
    67501 aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt
    67561 cttaccacgc cgatttgcag gcgaaaggaa tggaagttaa gtaatcctgt aataattact
    67621 tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt ttttgtatcg aacaagatat
    67681 tgagggagcg tcctgctcgc cacgtcactc tttatgagag agcttttcag cctgaagatc
    67741 aacgtctgcg ccttttttcg ccgcagcatc gtcatcattc atcgccttct tgaacccttt
    67801 aatggccgct ccaaggtctc cgcccagcgt acgtaacttc ttagtcccaa acagcagaac
    67861 gaccagcgcc gcaactacca gcagtttggt aatactaatc tcacccatag ataccttctt
    67921 gacatataac cggcaacaga acgcgcctta aaaaccagag aatattaacg gtcattcgac
    67981 gcgcgcacac aatagcaatc tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat
    68041 aataattgcg gcggcgcaaa gcgacggttg tttaagatgg gcagttgcgc gcgcacctga
    68101 cgcacacgtt cgggcgtcac ttccgccata atgagtgcag gcatttctga cgctgccgca
    68161 atggtgacgc caaagggatc tataatccgg ctttgcccga tatttttgtt cccgcactcc
    68221 cccgccgcca ccatataaca ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag
    68281 tgatgctctt tgagcggccc acgaacccag gcggcaggaa gtaccaggat ttcagctccc
    68341 tgtaaagcct gtgccagcgc cagctctgga aagcgtaagt cataacaggt catcagaccg
    68401 accttcatcc cttccacctc cagtaacgga gctatttcat taccagcatc aacatggcgt
    68461 gattcctgaa tggcaaatgc atcatagaga tgcagtttgg cataacgggc gacgatgttt
    68521 cctgcctgaa gtgccaccag catattccat gcgcgccccg gcgttgaagg aagatgaatc
    68581 gtcagaattg tcgtcatcat gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg
    68641 ccttccagca gctgtgctga tttaaccgat agatctgcat catgatcatc gcgcgccagc
    68701 aatgcttccg gcaggacaaa cagcgatacg tcgttttccg ccgcctgcgc catcaacgag
    68761 gcacaaatct cagcgttctt ttcccacaca gatgtaacag caaactgtcc tgcagcaacc
    68821 agcattgcca cctcctgtca gagtcgtaaa attcgccaac gacgattatt cggcgttaca
    68881 cttatcactc atacaaatca aatagcagga ttttgcagtg ttacaacttc ttttagcagt
    68941 ttttattggc ggtggtacgg gaagcgtagc gagatggctg ttaagtatgc gatttaaccc
    69001 gctgcatcag gcgattccgt tggggacgct ggcagcaaac ctgattgggg cattcatcat
    69061 aggaatggga ttcgcctggt tcagcaggat gacgaacatt gatccagtgt ggaaagtatt
    69121 aatcaccacc ggattttgtg gcggtctaac aaccttctca acattttcgg cagaagtggt
    69181 gtttttgtta caagagggcc gctttggctg ggcattactg aacgttttcg tcaaccttct
    69241 ggggtctttt gccatgaccg cactggcatt ctggctgttt tcggcctcaa ccgtacacta
    69301 aagaccaaaa aaaacccgct gattaagcgg gttttgaatt cttgctgacg tatcttacag
    69361 agcgattacg tttgcagcag aagggccttt ggcaccgtta gtgatttcga actctacgcg
    69421 ctgaccttca gcaagagttt taaaaccatt agtctggatt gcagagaagt gtacgaacac
    69481 gtctttgctg ccgtcttccg gagtaatgaa accgaatcct ttggactcat taaaccactt
    69541 aacgttacct ttaatcttag acatcaaaat tacctttaca tgaaaaatag acacaaatgc
    69601 tgtgtcggtt atcagtacac caattgtggt acgctttgtc cagtgcaact ttactaaaaa
    69661 gtgataaaag tcgcgttatt tttgatgttg cttattttag ttattgattt taaagaagtt
    69721 actaaaactt catttgtctc aaaactgaaa gcgcatccag gcaaagtaca cattgccatt
    69781 gttgtaggta cccggaatgt aggtcatctg aaaagtcact gggccataac ccacggaggc
    69841 caatggcagt agaaccggga gagggatgta attccagtta tcgcgtgccg ttacgccagc
    69901 ggtgaatccc agacctaaat gaaaattttc atccgccagc ggtcgccagg tactttccca
    69961 tccgtatccg gcaatcggtt cccatttgtt ccacgagtcc ttaaatgcca tggcatacag
    70021 gccatgccag tttccttttt catcccaacg cgacaggcca aaaccgccac cccacggtcg
    70081 ctcgttatag cgatcggttt tttctttgtc gtaagcgaaa cgtgcatgcc aggtgatggc
    70141 aggaatatat aaatcataat gttcaggctg ttgccaggtt tgtgcaatat tttctctaaa
    70201 cgttgtcatc ctctcatctg cgttagcaaa aactttacca acgctgatta actgaataaa
    70261 aacaaaggaa aagatagcga catatttact cacgttcatt tgtgaccata aaacatttat
    70321 caaaaatcta ctactagcat agcaaagcta caattaacat aaccttaata gacccaacat
    70381 aaagaataat ctgaatagct gctctattta tttccgcttg ttcaagttaa atatttccgc
    70441 gcatcgatta aagatgatgc gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca
    70501 agtctttttt gatgacaaaa tgcaatcaag gaaaaattat tttatttttt taacgagata
    70561 caacaatcat cttaacgaag tatataatat atatcgtcaa cccatgctca tattagatta
    70621 ttttttgagc gcacagccgc agctaacacc attgcaatta acaaatttgc atcaatccac
    70681 catcaattta cacacattat tatgtgataa ttgccaaccg ctaaatatgc gttttgttat
    70741 ctatgtataa aaacagcaac ttcaatgtct taatggcagt ttttcttgat tttaatcagc
    70801 attcatcgcc aatttattgg gcatattttt tccttaagct ttaggaagtt tttattattt
    70861 actttgggcc tggagacagg aaaaattatg ctgacattca ttgaactcct tattggggtt
    70921 gtggttattg tgggtgtggc tcgctacatc attaaagggt attccgccac tggtgtgtta
    70981 tttgtcggtg gcctgttatt gctgattatc agtgccatta tggggcacaa agtgttaccg
    71041 tccagccagg cttcaacagg ctacagcgcc acggatatcg ttgaatacgt taaaatatta
    71101 ctaatgagcc gcggcggcga cctcggcatg atgattatga tgctgtgtgg ctttgccgct
    71161 tacatgaccc atatcggcgc gaatgatatg gtggtcaagc tggcgtcaaa accattgcag
    71221 tatattaact ccccttacct gctgatgatt gccgcctatt ttgttgcttg tctgatgtca
    71281 ctggccgtct cttccgcaac cggtctgggg gttttgctga tggcaaccct gtttccggtg
    71341 atggtaaacg ttggtatcag tcgtggcgca gcagctgcca tttgtgcctc cccggcggcg
    71401 attattctcg caccgacttc aggggatgtg gtgctggcgg cgcaggcttc cgaaatgtcg
    71461 ctgattgact tcgccttcaa aacgacgctg cctatctcaa ttgctgcaat tatcggcatg
    71521 gcgatcgccc acttcttctg gcaacgttat ctggataaaa aagagcacat ctctcatgaa
    71581 atgttagatg tcagtgaaat caccaccact gcccccgcgt tttatgccat tttgccgttc
    71641 acgccgatca tcggtgtact gatttttgac ggtaaatggg gtccgcaatt acacatcatc
    71701 actattctgg tgatttgtat gctgattgcc tccattctgg agttcctccg cagctttaat
    71761 acccagaaag ttttctctgg tctggaagtg gcttatcgcg ggatggcaga tgcgtttgct
    71821 aacgtggtga tgctgctggt tgccgctggg gtattcgctc aggggcttag caccatcggc
    71881 tttattcaaa gtctgatttc tatcgctacc tcgtttggtt cggcgagtat catcctgatg
    71941 ctggtattgg tgattctgac catgctggcg gcagtcacga ccggttcagg caatgcgccg
    72001 ttttatgcgt ttgttgagat gatcccgaaa ctggcgcact cttccggcat taacccggcg
    72061 tatttgacta ttccgatgct gcaggcgtca aacctgggtc gtaccctatc acccgtttct
    72121 ggcgtagtcg ttgcggttgc cgggatggcg aaaatctcac catttgaagt cgtaaaacgc
    72181 acctcggtgc cggtgcttgt tgggctagtg attgttatcg ttgctacaga gctgatggtg
    72241 ccaggaacgg cagcagcggt cacaggcaag taaatagtaa tgccggaggc aagtttcctc
    72301 cggctttatc atgtcaccca ctgtggtata tgcgttgtgg tctgccaact ttgccgtgaa
    72361 caatttcagc aataatcaga tggcggctgg cgcaatattc aagataacgc ctggcagtgg
    72421 tgcggctgat ggtcagtgcc tgcgccaccg tttctgccgt atgttgcaca ccaggctctt
    72481 taaacagttt tcgcaccgcg tttagcgtca aggggtcaat gccggtcggc agttcgtctt
    72541 tgggttcacc gcgagcatag gcattaaaca tctcatcaat ttgcttctgg ctggcgctat
    72601 caatactttc cagcatatgt ttacgctggc ggaaacgggt tagcgtttgc cccagtgtat
    72661 tgattcactt gaagtacgaa aaaaccggga gaacattgga ttattcggga tctgatggga
    72721 ttagatttgg tggggcttgc aagcctgtag tacaaatttt aggcgttaac caatgaaacg
    72781 cgaaagatag taaaaaattg cttttgtttc attgaaaaaa cgaaaaacaa aaacactata
    72841 aatcatttca atcatagctt caaaaaacgt tcaaaaccga taacaaccag gctgtcatca
    72901 aatgactcat atcacaaatc agctcatgcc gtttaggtat gttacatgtg tgattatgtg
    72961 aggtgaagta tgttttagct ggctcatggt tgttatacgg cttttttcgc atcttgtggt
    73021 tccaacaaag gagctacagg ctcttcctgt ttatgaacac catacattga caaaatctct
    73081 tggcgagttt ctggaggtaa tttatgcagc gccaaagctg tttgtagtgt ttggcgatca
    73141 atacccagcg ttgccaatag ggtgtcaacg gagatggagg ggcgttcttg cattaatccg
    73201 gaaagcccat atttagccat gtttagctct atctctcgga cttcattttc tcctagttta
    73261 ttgattaaca gggagaacat tttcaatgta tcagcattca tttcttcgat agcaattttt
    73321 tgcatagccg gtattgccgg gatatattca accccaggcc cgcgacgccc ttcttgtgaa
    73381 cgcttcggcc atccttcttt ctttgctcgt ttatgcaccc cctgtattgt cccaggcatt
    73441 ccatccagtt cagtaatttg tttgggtgtc atccactggt ttatctcatt tgcgcttttc
    73501 ataaactaag tttaccagat ttatctatat aaaaatcaat ggattacaaa ataaaccaca
    73561 ttaactgata taaaccggtt tggtttattt ggtaatgtat aaaacagata atcaagacga
    73621 attatttgtt ttggtaaccc tttatcatac gcaatatggt tggtgtgcga aagatgagta
    73681 acgattggca tcgggccgac gtgattgcgg ccctcaaaaa aacaggaacc cctctggcaa
    73741 aattgtccag acgttcaggt tattcaaccg ggacgcttgc aaatgcatta gtcaggccat
    73801 ggcctaaagg tgaacgcatt attgctgacg ccttaaacat gaaacctgag gaaatttggc
    73861 ctagtagata tctaagggat cacaatgaga gagtggatat cagcaattga atgcatgggt
    73921 ttatctggtt tacctggttc attaatgggg gttcacaaaa gagctcagag agaaggatgg
    73981 ctcaagagaa gaagagaagg aattagaggg gttgcttacg aataccatat atcttctctt
    74041 cctaacattg ctcgagctga agttttgctc caacaaggaa aatttaaaac cagtcaggga
    74101 tgttttgaaa tcgcccgccc cacgctggaa gctcatgatt atgatcgtga ggcactatgg
    74161 agcaaatggg ataaagccag cgattcccag cgcagcctgg ctgaaaaatg gttgccctca
    74221 attcaggcca cagacgaaat gctgaaccag gggatttcaa cgaaaacggc ttttgcgacc
    74281 gttgcagggc attaccaggt cagcgcatcc actttgcggg acaagtatta ccaggtacag
    74341 aagtttgcga agcctgactg ggcggctgca cttgttgatg gacgtggagc atcccgtcgc
    74401 aatgttcaca aaagtgaatt tgacgaggat gcctggcagt ttctgattgc agattatctg
    74461 cgaccggaaa aacccgcctt ccgcaaatgt tatgagcgtc tggaactggc agcccgcgag
    74521 catggctgga gtattccctc ccgtgccacg gcctttcgcc ggattcagca actggacgag
    74581 gcaatggttg ttgcctgtcg tgaaggtgaa catgcactga tgcatctgat accggcacag
    74641 cagcgaactg tggaacacct ggacgccatg cagtggatca acggcgacgg ttatctgcat
    74701 aacgtctttg tacgctggtt taacggtgat gtgatccgtc cgaaaacatg gttctggcag
    74761 gatgtgaaaa cccgaaaaat tctgggctgg cgctgcgatg tgagcgagaa cattgattca
    74821 attcgcctct cgttcatgga tgttgtgact cgctacagta tcccggagga ttttcacatc
    74881 accattgata acacccgtgg tgctgcgaat aaatggctga cgggaggcgc gcccaatcgc
    74941 taccgcttta aggtaaaaga ggacgatcca aaaggactgt ttttactgat gggtgcgaaa
    75001 atgcactgga caagcgttgt tgccggtaaa ggctggggcc aggcaaaacc tgttgaacgt
    75061 gctttcggtg ttggtgggct tgaggaatac gttgataagc atccggcact ggctggcgca
    75121 tatacggggc caaatccgca ggcaaaacct gataactatg gcgaccacgc tgttgatgca
    75181 gagctgtttc tgaaaacctt tgccgaaggt gtggcgatgt tcaatgccag aacaggccgt
    75241 gaaacagaaa tgtgcggggg caaactctcg tttgatgatg ttttcgagcg tgaatacgcc
    75301 agaacgattg tgcgtaagcc aaccgaagaa caaaaacgga tgctgttact gcctgccgag
    75361 gcggtgaacg tttcacgcaa aggcgagttt acgcttaaag ttggcggctc ccttaaaggc
    75421 gcgaaaaacg tttattacaa catggcatta atgaatgccg gagtgaaaaa agttgtggtc
    75481 agatttgatc cacagcagtt acacagcacg
//
LOCUS       DAJVQR010000004        72906 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVQR010000004 DAJVQR010000000
VERSION     DAJVQR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM52_000276"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM52_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1562262.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQM52_000277"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQM52_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1562263.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQM52_000278"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQM52_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1562264.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQM52_000279"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQM52_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1562265.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQM52_000280"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQM52_000280"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1562266.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQM52_000281"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQM52_000281"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1562267.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQM52_000282"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQM52_000282"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1562268.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQM52_000283"
     CDS             complement(4598..5320)
                     /locus_tag="OQM52_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1562269.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQM52_000284"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQM52_000284"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1562270.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OQM52_000285"
     CDS             6520..6738
                     /locus_tag="OQM52_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1562271.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQM52_000286"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQM52_000286"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1562272.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OQM52_000287"
     CDS             complement(7472..7672)
                     /locus_tag="OQM52_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1562273.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OQM52_000288"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OQM52_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OQM52_000289"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OQM52_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1562274.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OQM52_000290"
     CDS             10101..12014
                     /locus_tag="OQM52_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1562275.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OQM52_000291"
     CDS             12014..13036
                     /locus_tag="OQM52_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1562276.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OQM52_000292"
     CDS             13030..13248
                     /locus_tag="OQM52_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1562277.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OQM52_000293"
     CDS             13302..14171
                     /locus_tag="OQM52_000293"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1562278.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OQM52_000294"
     CDS             complement(14226..14630)
                     /locus_tag="OQM52_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1562279.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OQM52_000295"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OQM52_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1562280.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OQM52_000296"
     CDS             15615..17705
                     /locus_tag="OQM52_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1562281.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OQM52_000297"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OQM52_000297"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1562282.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OQM52_000298"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OQM52_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1562283.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OQM52_000299"
     CDS             complement(19673..20275)
                     /locus_tag="OQM52_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1562284.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OQM52_000300"
     CDS             complement(20265..20432)
                     /locus_tag="OQM52_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1562285.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OQM52_000301"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OQM52_000301"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1562286.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OQM52_000302"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OQM52_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1562287.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OQM52_000303"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OQM52_000303"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1562288.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OQM52_000304"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OQM52_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1562289.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OQM52_000305"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OQM52_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1562290.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OQM52_000306"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OQM52_000306"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1562291.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OQM52_000307"
     CDS             28269..28436
                     /locus_tag="OQM52_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1562292.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OQM52_000308"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OQM52_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OQM52_000309"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OQM52_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1562293.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OQM52_000310"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OQM52_000310"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1562294.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OQM52_000311"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OQM52_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1562295.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OQM52_000312"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OQM52_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1562296.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OQM52_000313"
     CDS             complement(33764..34849)
                     /locus_tag="OQM52_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1562297.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OQM52_000314"
     CDS             complement(34861..36165)
                     /locus_tag="OQM52_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1562298.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OQM52_000315"
     CDS             complement(36177..36530)
                     /locus_tag="OQM52_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1562299.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OQM52_000316"
     CDS             complement(36541..37419)
                     /locus_tag="OQM52_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1562300.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRITVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OQM52_000317"
     CDS             complement(37416..38642)
                     /locus_tag="OQM52_000317"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1562301.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OQM52_000318"
     CDS             complement(38643..39806)
                     /locus_tag="OQM52_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1562302.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OQM52_000319"
     CDS             complement(39890..40252)
                     /locus_tag="OQM52_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1562303.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OQM52_000320"
     CDS             complement(40269..41174)
                     /locus_tag="OQM52_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562304.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OQM52_000321"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OQM52_000321"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1562305.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OQM52_000322"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OQM52_000322"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1562306.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OQM52_000323"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OQM52_000323"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1562307.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OQM52_000324"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OQM52_000324"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1562308.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OQM52_000325"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OQM52_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1562309.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OQM52_000326"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OQM52_000326"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1562310.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OQM52_000327"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OQM52_000327"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1562311.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OQM52_000328"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OQM52_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1562312.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OQM52_000329"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OQM52_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OQM52_000330"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OQM52_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1562313.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OQM52_000331"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OQM52_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1562314.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OQM52_000332"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OQM52_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1562315.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OQM52_000333"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OQM52_000333"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1562316.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OQM52_000334"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OQM52_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1562317.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OQM52_000335"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OQM52_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1562318.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OQM52_000336"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OQM52_000336"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1562319.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OQM52_000337"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OQM52_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1562320.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVEQNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OQM52_000338"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OQM52_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1562321.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61328)
                     /locus_tag="OQM52_000339"
     CDS             complement(59604..61328)
                     /locus_tag="OQM52_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1562322.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61707..63329
                     /gene="pckA"
                     /locus_tag="OQM52_000340"
     CDS             61707..63329
                     /gene="pckA"
                     /locus_tag="OQM52_000340"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1562323.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63405..64757)
                     /gene="envZ"
                     /locus_tag="OQM52_000341"
     CDS             complement(63405..64757)
                     /gene="envZ"
                     /locus_tag="OQM52_000341"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1562324.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64754..65473)
                     /gene="ompR"
                     /locus_tag="OQM52_000342"
     CDS             complement(64754..65473)
                     /gene="ompR"
                     /locus_tag="OQM52_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1562325.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65701..66177
                     /gene="greB"
                     /locus_tag="OQM52_000343"
     CDS             65701..66177
                     /gene="greB"
                     /locus_tag="OQM52_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1562326.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66274..68595
                     /locus_tag="OQM52_000344"
     CDS             66274..68595
                     /locus_tag="OQM52_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1562327.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69033..69260
                     /gene="feoA"
                     /locus_tag="OQM52_000345"
     CDS             69033..69260
                     /gene="feoA"
                     /locus_tag="OQM52_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1562328.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69277..71598
                     /gene="feoB"
                     /locus_tag="OQM52_000346"
     CDS             69277..71598
                     /gene="feoB"
                     /locus_tag="OQM52_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1562329.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71598..71834
                     /gene="feoC"
                     /locus_tag="OQM52_000347"
     CDS             71598..71834
                     /gene="feoC"
                     /locus_tag="OQM52_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1562330.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72037..>72906
                     /gene="rpnA"
                     /locus_tag="OQM52_000348"
     CDS             72037..>72906
                     /gene="rpnA"
                     /locus_tag="OQM52_000348"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCS1562331.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
BASE COUNT        17569 a        19754 c        18442 g        17141 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgatgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagcagaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccccagcgtg ttgccggtga aagttcaacg
    61321 ttatccatgt tggttatcca gaatcaaaag gtgggttaat tatcgcatcc gggcagtagt
    61381 attttgcttt tttcagaaaa taatcaaaaa acgttagcgt ggtgaatcga tactttaccg
    61441 gttgaatttg catcaatttc attcaggaat gcgattccac tcacaatatt cccgccatat
    61501 aaaccaagat ttaacctttt gagaacattt tccacaccta aaatgctatt tctgcgataa
    61561 tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc aaatatgaat ttctccagat
    61621 acgtaaatct atgagccttg tcgcggttaa cacccccaaa aagactttac tattcaggca
    61681 atacatattg gctaaggagc agtgaaatgc gcgttaacaa tggtttgccc ccgcaagaac
    61741 tcgaggctta tggtatcagt gacgtacatg atatcgttta caacccaagc tacgacctgc
    61801 tgtatcagga agagctcgat ccgagcctga caggttatga gcgcggggtg ttaactaatc
    61861 tgggtgccgt tgccgtcgat accgggatct tcaccggtcg ttcaccaaaa gataagtata
    61921 tcgtccgtga cgataccact cgcgatactt tctggtgggc agacaaaggc aaaggtaaga
    61981 acgacaacaa acctctctct ccggaaacct ggcagcatct gaaaggcctg gtgaccaggc
    62041 agctttccgg caaacgtctg ttcgttgtcg acgctttctg tggtgcgaac ccggatactc
    62101 gtctttccgt ccgtttcatc accgaagtgg cctggcaggc gcattttgtc aaaaacatgt
    62161 ttattcgccc gagcgatgaa gagctggcag gtttcaaacc agactttatc gttatgaacg
    62221 gcgcgaagtg cactaacccg cagtggaaag aacagggtct gaactccgaa aacttcgtgg
    62281 cgtttaacct gaccgagcgc atgcagctga ttggcggcac ctggtacggc ggcgaaatga
    62341 agaaagggat gttctcgatg atgaactacc tgctgccgct gaaaggtatc gcttctatgc
    62401 actgctccgc caacgttggc gagaaaggcg atgttgcggt gttcttcggc ctttccggca
    62461 ccggtaaaac caccctttcc accgacccga aacgccgcct gattggcgat gacgaacacg
    62521 gctgggacga tgacggcgtg tttaatttcg aaggcggctg ctacgcaaaa actatcaagc
    62581 tgtcgaaaga agcggaacct gaaatctaca acgctatccg tcgtgatgcg ctgctggaaa
    62641 acgtcaccgt gcgtgaagat ggcactatcg actttgatga tggttcaaaa accgagaaca
    62701 cccgcgtttc ttatccaatc tatcacatcg ataacatcgt taagccggtt tccaaagcgg
    62761 gccacgcgac taaggttatc ttcctgactg ctgatgcttt cggcgtgttg ccgccggttt
    62821 ctcgcctgac tgccgatcaa acccagtatc acttcctctc tggcttcacc gccaaactgg
    62881 ccggtactga gcgtggcatc accgaaccga cgccaacctt ctccgcttgc ttcggcgcgg
    62941 cgttcctgtc gctgcacccg actcagtacg cagaagtgct ggtgaaacgt atgcaggcgg
    63001 cgggcgcgca ggcttatctg gttaacactg gctggaacgg cactggcaaa cgtatctcga
    63061 ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg ttcgctggat aatgcagaaa
    63121 ccttcactct gccgatgttt aacctggcga tcccaaccga actgccgggc gtagacacga
    63181 agattctcga tccgcgtaac acctacgctt ctccggaaca gtggcaggaa aaagccgaaa
    63241 ccctggcgaa actgtttatc gacaacttcg ataaatacac cgacacccct gcgggtgccg
    63301 cgctggtagc ggctggtccg aaactgtaat gatttgaagc tggagaatat ctatccagta
    63361 tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt ttatttaccc ttcttttgtc
    63421 gtgccctgcg cccgcgttac cggcactggc agccaggcgc gaatggaaag cccgccccgc
    63481 tcgctggtgc caagctccag catcccgtta tggttatcca cgatacgctg cacaattgcc
    63541 agccctaatc ccgtgccgct aatggtgcgc gcactgtcgc cgcggacaaa cggctggaac
    63601 aggtgcttac gttgttccgg cgcaattccc ggaccgtcat cttccacctg gaaccaggcg
    63661 cgattcggct ccgttccgct gctgactttg atccagccat tgccataacg ggcggcgttg
    63721 accaccatat tcgccaccgc gcgtttgatc gacagcgggt gcattttcac ttcaatgctg
    63781 ccggggtaaa gcgcggtttc aatttcccgc tcatagccac tttcggcagc aatcacctca
    63841 ccgagcactg cattaagatc cgccatttcc atcggcatct cctgcccggt gcgcaggtag
    63901 tcgataaact gctcaatgat ggcgttgcac tcttcgatat ctttattgat cgattctgcc
    63961 agatagccat cctgctcgct catcatctca gtcgccaggc gaatacgcgt cagcggcgtg
    64021 cgcaagtcgt gacttacccc cgccatcagc agcgtgcggt catccgccag ttgcttaaca
    64081 ccagccgcca tatggttaaa ggcacgggta acggaacgca cctccgaagc gccatattca
    64141 cgcagcggcg gcggaataat ccctttacca acctgcaagg ctgcgtgttc gagatcgacc
    64201 aacggtcggt tctggatacg aataaacagc cacgccccgc ctatcgccaa tagcataatc
    64261 gccagcgtat agcggaacag cggagagaaa tcgccctgat gaatttcggt cagcggcacg
    64321 cgtacccaga tattgggcga cagccaggtt ttcagccaga cgacaggcga acttttgttg
    64381 acctcaacgc gcacttccgt cgggccgccc agttgctgtg ccatctgatg gcttaagaat
    64441 tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag cctcgttgga gtagagagag
    64501 atccccagct cacggtagat ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg
    64561 tcctccagtt gcagtttgtc ggtcatcaac atacgcactt cgtacgcgag gactttatta
    64621 aactgctgga ggctcggcaa aatcgcgaag ttcagcacca ccagataagt cgtcaccagg
    64681 ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg caaatgaact tcgtggcgag
    64741 aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa gacgtagccc aggccccaaa
    64801 cggtctgaat gtaacgcgga tgcgctggat cttcttctac catgcggcgc agacgcgaaa
    64861 tctgcacgtc gatggagcgt tccattgcgg aatattcacg accacgggca aggttcatca
    64921 gcttatcgcg ggagagcggc tcacgcggat ggctgaccag tgccttcagt accgcaaact
    64981 caccgctggt gagcggcatc ggctcatctt cgcggaacat ttcgcgcgta ccgaggttaa
    65041 gtttgaactt accgaaagca attaccgcct cttcctgtga cggtgcgcct ggcagttcgt
    65101 tcgcctgacg acgcagcacc gcacgaatac gggccagcag ttcacgcggg ttaaacggtt
    65161 ttggaatgta gtcgtcagcg ccaatctcca ggcctacgat acggtccact tcttcccctt
    65221 tcgccgtcac cataatgatc ggcatcgggt tgctctgact acgaagacgt cggcaaatcg
    65281 acaagccatc ttcaccaggt aacattaaat ccagtaccat aagatggaaa gattcacgag
    65341 tcagcaggcg atccatctgt tctgcattag cgacgcttcg aacctggaag ccttgttcgg
    65401 tgagataacg ttccagcagc gcacgcaggc gcatgtcgtc atcgaccacc agaatcttgt
    65461 agttctcttg cattgtttgt actcccaaag gttcgcaaca atttgtaagc gtgtattctt
    65521 aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa tttcagccta aattgttaca
    65581 aagcatatta aacagcagct taagtataca atttattcgg cgaaacatta ttgattctgt
    65641 tgatatgatc acgttatacc caatgtgcgc attatcaaac agacaaaggg aatcaacgag
    65701 atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat
    65761 ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc
    65821 gaccgcagcg aaaatgctga ctatcagtat aataaaaagc gtctgcgtga aatcgaccgt
    65881 cgcgtgcgct atctcactaa atgcctggaa aatctcaaaa tcgtcgatta ctcccctcag
    65941 caggaaggca aagtcttttt tggcgcgtgg gtggagattg aaaacgacga tggcgtgact
    66001 caccgtttcc gtattgtcgg ctacgatgaa atttttggcc gtaaagatta catctctatc
    66061 gattccccga tggcccgcgc attgctgaaa aaagaagtcg gcgatctggc ggtggtgaat
    66121 acccctgccg gggaagcgaa ctggtatgtt aatgctatcg agtacgtgaa accgtaagga
    66181 agagtcttaa cctcctgccg atggctggca tttttgccag ccagtccgta taactatccc
    66241 ctgatttttg atccgaaaag atgaactcaa accatgatga atgattcgtt ctgccgcatt
    66301 attgcgggtg aaattcaggc gcgcccggaa caggttgacg ctgccgttcg cctgcttgac
    66361 gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat
    66421 gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga
    66481 cgtcaggcga tcctcaaatc catttccgag caaggcaaac tcaccgatga tctggcgaag
    66541 gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct
    66601 aaacgccgca cccgcgggca aatcgccatt gaagcagggc ttgagccgtt ggctgacctg
    66661 ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg cacaatatgt tgatgccgat
    66721 aaaggcgtgg cagataccaa agccgcgctg gatggcgcgc gctatatcct gatggaacgg
    66781 tttgccgaag atgccacgct gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat
    66841 ttggtttcta cggtggtgag cggtaaagaa gaggaagggg cgaaattccg cgactatttc
    66901 gatcatcacg aaccgttgtc cacggtgcct tctcaccgcg cgctggcgat gttccgtggg
    66961 cgtaacgaag gcgtactcca gctttcgctg aatgccgatc cgcagttcga tgagccgccc
    67021 aaagagagct attgcgagca aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc
    67081 ccggcggata gctggcgcaa aagcgtagtg agctggacct ggcgcatcaa ggtgctgatg
    67141 catctggaaa ccgaactgat gggtaccgtg cgcgaacgcg cggaagatga agcaatcaac
    67201 gtctttgccc gtaacctgca cgatctgctg atggcggccc ctgccggact gcgtgcaacg
    67261 atgggcctcg atccgggtct gcgtaccggg gtaaaagtgg cggtggtcga tgccactggc
    67321 aaactggtgg cgaccgatac catttacccg cacaccggac aagccgcaaa agcagcgatg
    67381 accgttgctg ccttgtgtga aaaacataac gttgaactgg tggcgatcgg caacggtaca
    67441 gcttcccgcg aaactgaacg tttctatctc gacgtgcaga agcagttccc gaaagtgacc
    67501 gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt actcagcttc cgagctggca
    67561 gcgcaggagt tcccggatct cgacgtttcg ctgcgtggtg cggtatctat cgcccgccgt
    67621 ttgcaggatc cgctggcgga gctggtgaaa atcgatccga aatctatcgg cgtaggtcag
    67681 tatcagcatg acgtcagcca gacgcaactg gcccgcaaac tggatgcagt agtagaagac
    67741 tgcgtaaacg ccgttggcgt cgatctcaac accgcttctg ttccgctatt aacccgcgtg
    67801 gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag
    67861 ttccagaacc gtcagcaact gctgaaagtc agccgtctgg ggccgaaagc cttcgagcag
    67921 tgcgcgggct tcctgcgcat taaccacggt gataacccgc tggacgcctc taccgttcat
    67981 ccggaagcct atccggtggt ggaacgcatt ctggcagcaa cacagcaggc actgaaagat
    68041 ctgatgggta acagcagcga actgcgtaac ctgaaagcgt ctgactttac tgatgaaaaa
    68101 ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg aaaaaccggg tcgcgatccg
    68161 cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa
    68221 ccgggtatga tcctcgaagg cgcagtgacc aacgtcacca actttggcgc gtttgtcgat
    68281 attggcgtgc atcaggacgg cctggttcac atctcttcat tgtcgaacaa gtttgtggaa
    68341 gatccgcata ccgtggtgaa agcgggcgac attgtgaagg tgaaagtgct ggaagtggat
    68401 cttcagcgta aacgtatcgc cctgactatg cgtctggacg agcagcctgg cgaaaccaac
    68461 tctcgtcgcg gcggcggtaa tgaacgcccg caaaacaacc gcccggcagc caaaccacgc
    68521 ggtcgtgaag cgcagcctgc cggtaatagc gcgatgatgg atgcgctggc ggcagcgatg
    68581 ggtaaaaaac gttaaacgcc cggactggcc cctaaacctg cgcgataagg cgttcacgcc
    68641 gcatctggca acgaacgctt tgcctgacat aaaagtgccg gagaatatct ccggcacttt
    68701 tattccacag ccaaactcac aatatactcc gttaatattt atcacttcat taacaactga
    68761 aaccttaatt aaacattagc cagtccgggt aactcactat tcgaattata ttttcgctgc
    68821 gatataacct tgagccacat caacattgag tcagattatt attcaaacca acattcgcac
    68881 acattttaag tattgctgat agaaaccatt ctcattatca ttgtgttgtt gattatttaa
    68941 tctctccttt gttggcaaat catctggtct catgtcgcgg tcaaacgccc catgaggtag
    69001 ttatccagtt aatgagaaac aagtaggcac ctatgcaata cactccagat actgcgtgga
    69061 aaatcactgg cttttcccgt gaaatcagcc cggcatatcg ccaaaaactg ctttctctag
    69121 gcatgttacc tggctcctct tttgatgtgg tgcgcgtcgc tccactcggc gaccccattc
    69181 atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa agatctggcc ttattagaag
    69241 tggaagcggt ttcctgttaa tacggtgata acaacaatga aaaaattaac cattggctta
    69301 attggtaatc caaattctgg caagacaacc ttatttaacc agctcactgg cgcacgtcag
    69361 cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc
    69421 gatcatcagg tcacgctggt ggacctgccc ggcacctatt ctctgaccac catctcatcg
    69481 cagacctcgc tcgatgagca aatcgcctgt cactacattt tgagtggcga cgccgacctg
    69541 ctgattaacg tggtggatgc gtctaacctt gagcgtaacc tgtacctgac gctgcaactg
    69601 ctggaactcg gcattccctg catcgtggcg ctgaacatgc tcgacattgc cgagaagcaa
    69661 aatattcgta ttgaaattga tgctctatcg gcacgtcttg gctgcccggt gatcccgctg
    69721 gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg cgattgatcg ctataaagct
    69781 aacgagaatg tggaactggt gcattacgca cagccgctac tcaacgaagc agattcactg
    69841 gcaaaagtga tgccttccga catcccgctg aaacaacgtc gctggctggg cctgcaaatg
    69901 ctggaaggcg atatctacag ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc
    69961 accctcgccc gtctgcgtaa tgaaatggat gatccggcgc tgcacattgc cgatgcgcgt
    70021 taccagtgca ttgctgccat ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc
    70081 cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc
    70141 ctctttgtga tgtacctgat gttcctgctg gctatcaaca tcggcggggc gttacagccg
    70201 ctgtttaacg tcggctccgt ggcgctattt gtgcatggta ttcaatggat tggctacacg
    70261 ctccacttcc cggactggct gactatcttc ctcgcccagg gcctgggtgg cggtattaac
    70321 accgtgctgc cactggtgcc gcagatcggc atgatgtacc tgttcctctc cttccttgag
    70381 gactccggct atatggcgcg tgcagcgttt gtgatggacc gcctgatgca ggcgctgggc
    70441 ttgccgggga aatcctttgt gccgctgatc gtcggtttcg gttgtaacgt accgtcggta
    70501 atgggcgcac gtacgcttga tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg
    70561 tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat tcgcggctgc cttcttcggg
    70621 cagaacggtg ccctggcggt tttctcgctg tatatgctcg gtatcgtgat ggcggtgctg
    70681 actggcctga tgctcaagta caccatcatg cgcggtgaag cgacgccgtt tgtcatggag
    70741 ctgccggtct accatgtacc acacgttaaa agcctgatta tccagacctg gcagcgtctg
    70801 aagggcttcg ttctgcgtgc tggtaaagtg attatcatcg tcagcatttt cctgagcgct
    70861 ttcaacagct tctcgttgag cgggaaaatc gtcgataaca tcaacgactc ggcgctggct
    70921 tccgtcagcc gggtgataac ccctgtcttc aagccgattg gcgtacatga agataactgg
    70981 caggcaacgg ttggcctgtt tacgggcgca atggcaaaag aagtggtggt gggtacgctc
    71041 aacaccctct acaccgcaga aaatattcag gacgaagagt tcaatccggc ggaattcaac
    71101 ctcggtgaag agctgttcag tgcggtagat gaaacctggc agagcctgaa agacaccttc
    71161 agccttagcg tactgatgaa ccccattgaa gctagcaaag gcaacggtga aatgggtacc
    71221 ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag ctgccgcata cagctacctg
    71281 attttcgtcc tgctgtatgt accatgtatc tcggtgatgg gagccatcgc ccgtgaatca
    71341 agccgtggct ggatgggctt ctccatcctg tgggggctga atatcgctta ctcactggca
    71401 acattgttct atcaggtcgc cagctacagt cagcacccaa cttacagcct ggtgtgcatt
    71461 ctggcggtaa tcctgtttaa catcgtagct atcggtctgc tgcgccgcgc gcgtagccgg
    71521 gtggatatcg aactgctggc aacccgcaag tcggtaagca gttgctgcgc agccagcacc
    71581 accggtgatt gccattaatg gcttcactta ttcaggtacg tgatttgctg gcgttacggg
    71641 gccgtatgga agcgacccag ataagccaga cattgaacac tccgcagcca atgattaacg
    71701 ccatgctgca acagctggaa agcatgggca aagccgtgcg gattcaggaa gaacctgacg
    71761 gctgcctctc tggcagttgt aaaagctgcc cggaaggcaa agcctgtctg cgcgagtggt
    71821 gggcgctgcg ttaaccatac tccatcgcct gttttgaaaa gcccggtatg cgtttgcatc
    71881 cgggcttttt tgcgtgcggc tttccataaa aatgcaattc ttgcagcgca acgtaagttc
    71941 ctctgaaagc gtctcgcagg gatgaaaact cgctaatgca cagatgtgga gtggcgcgta
    72001 gagtcgcggc attcaaacaa caggtgaagg aacgccatga gtaaaaagca gagttccacc
    72061 ccacatgatg cgctgttcaa actcttttta cgccaaccgg agacggcacg cgattttctt
    72121 gcgtttcatt taccggcacc cattcacgcg ctctgtgata tgaaaaccct caagctggag
    72181 tcgaggagct ttattgatga cgatctgcgt gaaagctatt ccgatgtgct gtggtcggtg
    72241 aaaacggaac aaggaccagg atacatctat tgtctgattg aacatcaaag cacctcaaac
    72301 aaactgatcg catttcgcat gatgcgttac gctattgccg caatgcaaaa tcaccttgat
    72361 gctggataca aaacgttgcc gatggtggtg ccattgttgt tttaccacgg tattgaaagc
    72421 ccctatccct attcgctgtg ttggctggat tgtttcgccg atcccaaact ggcaaggcag
    72481 ctttatgcct ccgcatttcc gctgattgat gtcaccgtca tgcctgatga tgaaatcatg
    72541 cagcaccgac gtatggcgct gctggagtta attcaaaaac atattcgtca acgcgatctg
    72601 atggggctgg tagagcaaat ggcctgctta ttaagtagtg gatacgctaa tgacagacaa
    72661 atcaaagggc tgtttaatta catactgcaa actggcgacg ctgtacgttt taacgatttt
    72721 atcgacggcg ttgccgaacg ttcaccgaaa cacaaggaga gtttaatgac tattgcggaa
    72781 agattgcggc aggaggggga acaatccaaa gccctgcata tagccaaaat aatgcttgaa
    72841 tccggagtcc ctcttgcaga catcatgcga tttaccgggc tgtcagaaga agagttggct
    72901 gcggcg
//
LOCUS       DAJVQR010000005        71475 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVQR010000005 DAJVQR010000000
VERSION     DAJVQR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(750..2336)
                     /locus_tag="OQM52_000349"
     CDS             complement(750..2336)
                     /locus_tag="OQM52_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCS1562332.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="OQM52_000350"
     CDS             complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="OQM52_000350"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCS1562333.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4884..5288)
                     /locus_tag="OQM52_000351"
     CDS             complement(4884..5288)
                     /locus_tag="OQM52_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCS1562334.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="OQM52_000352"
                     /pseudo
     CDS             complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="OQM52_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="OQM52_000353"
     CDS             complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="OQM52_000353"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCS1562335.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="OQM52_000354"
                     /pseudo
     CDS             complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="OQM52_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9325..10095
                     /gene="glcC"
                     /locus_tag="OQM52_000355"
     CDS             9325..10095
                     /gene="glcC"
                     /locus_tag="OQM52_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCS1562336.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10219..11360)
                     /locus_tag="OQM52_000356"
                     /pseudo
     CDS             complement(10219..11360)
                     /locus_tag="OQM52_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11722..13452
                     /locus_tag="OQM52_000357"
     CDS             11722..13452
                     /locus_tag="OQM52_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCS1562337.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13449..14363
                     /locus_tag="OQM52_000358"
     CDS             13449..14363
                     /locus_tag="OQM52_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCS1562338.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14396..14644
                     /locus_tag="OQM52_000359"
     CDS             14396..14644
                     /locus_tag="OQM52_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1562339.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14644..15815
                     /locus_tag="OQM52_000360"
                     /pseudo
     CDS             14644..15815
                     /locus_tag="OQM52_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="OQM52_000361"
     CDS             complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="OQM52_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1562340.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="OQM52_000362"
     CDS             complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="OQM52_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1562341.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18028..18588)
                     /locus_tag="OQM52_000363"
     CDS             complement(18028..18588)
                     /locus_tag="OQM52_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1562342.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18600..19424)
                     /locus_tag="OQM52_000364"
     CDS             complement(18600..19424)
                     /locus_tag="OQM52_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562343.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19424..20773)
                     /locus_tag="OQM52_000365"
     CDS             complement(19424..20773)
                     /locus_tag="OQM52_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1562344.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20819..21577)
                     /locus_tag="OQM52_000366"
     CDS             complement(20819..21577)
                     /locus_tag="OQM52_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562345.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21609..22322)
                     /locus_tag="OQM52_000367"
     CDS             complement(21609..22322)
                     /locus_tag="OQM52_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1562346.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22496..23188
                     /locus_tag="OQM52_000368"
     CDS             22496..23188
                     /locus_tag="OQM52_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1562347.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="OQM52_000369"
     CDS             complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="OQM52_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS1562348.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25028..26887)
                     /gene="gss"
                     /locus_tag="OQM52_000370"
     CDS             complement(25028..26887)
                     /gene="gss"
                     /locus_tag="OQM52_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1562349.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27092..27958
                     /gene="yghU"
                     /locus_tag="OQM52_000371"
     CDS             27092..27958
                     /gene="yghU"
                     /locus_tag="OQM52_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1562350.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="OQM52_000372"
     CDS             complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="OQM52_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1562351.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="OQM52_000373"
     CDS             complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="OQM52_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1562352.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="OQM52_000374"
     CDS             complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="OQM52_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1562353.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29278..29772)
                     /locus_tag="OQM52_000375"
     CDS             complement(29278..29772)
                     /locus_tag="OQM52_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1562354.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="OQM52_000376"
     CDS             complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="OQM52_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1562355.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="OQM52_000377"
     CDS             complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="OQM52_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1562356.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="OQM52_000378"
     CDS             complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="OQM52_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1562357.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="OQM52_000379"
     CDS             complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="OQM52_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1562358.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34936..35223)
                     /locus_tag="OQM52_000380"
     CDS             complement(34936..35223)
                     /locus_tag="OQM52_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1562359.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35342..36229)
                     /locus_tag="OQM52_000381"
     CDS             complement(35342..36229)
                     /locus_tag="OQM52_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1562360.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36386..37426
                     /gene="gpr"
                     /locus_tag="OQM52_000382"
     CDS             36386..37426
                     /gene="gpr"
                     /locus_tag="OQM52_000382"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1562361.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37466..37960)
                     /locus_tag="OQM52_000383"
     CDS             complement(37466..37960)
                     /locus_tag="OQM52_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1562362.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38151..39035
                     /gene="yghA"
                     /locus_tag="OQM52_000384"
     CDS             38151..39035
                     /gene="yghA"
                     /locus_tag="OQM52_000384"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1562363.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="OQM52_000385"
     CDS             complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="OQM52_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1562364.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="OQM52_000386"
     CDS             complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="OQM52_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1562365.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40725..41912
                     /gene="metC"
                     /locus_tag="OQM52_000387"
     CDS             40725..41912
                     /gene="metC"
                     /locus_tag="OQM52_000387"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1562366.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42052..42711
                     /locus_tag="OQM52_000388"
     CDS             42052..42711
                     /locus_tag="OQM52_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1562367.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42751..43650)
                     /gene="yqhC"
                     /locus_tag="OQM52_000389"
     CDS             complement(42751..43650)
                     /gene="yqhC"
                     /locus_tag="OQM52_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1562368.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43844..45007
                     /gene="yqhD"
                     /locus_tag="OQM52_000390"
     CDS             43844..45007
                     /gene="yqhD"
                     /locus_tag="OQM52_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1562369.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45112..45939
                     /gene="dkgA"
                     /locus_tag="OQM52_000391"
     CDS             45112..45939
                     /gene="dkgA"
                     /locus_tag="OQM52_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1562370.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46139..47065
                     /locus_tag="OQM52_000392"
     CDS             46139..47065
                     /locus_tag="OQM52_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1562371.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47116..47373
                     /gene="yqhH"
                     /locus_tag="OQM52_000393"
     CDS             47116..47373
                     /gene="yqhH"
                     /locus_tag="OQM52_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1562372.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47416..49635)
                     /locus_tag="OQM52_000394"
     CDS             complement(47416..49635)
                     /locus_tag="OQM52_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1562373.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="OQM52_000395"
     CDS             complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="OQM52_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1562374.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="OQM52_000396"
                     /gene_synonym="parF"
     CDS             complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="OQM52_000396"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1562375.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52204..54462)
                     /gene="parC"
                     /locus_tag="OQM52_000397"
     CDS             complement(52204..54462)
                     /gene="parC"
                     /locus_tag="OQM52_000397"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1562376.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55008..55490)
                     /locus_tag="OQM52_000398"
     CDS             complement(55008..55490)
                     /locus_tag="OQM52_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCS1562377.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="OQM52_000399"
     CDS             complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="OQM52_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1562378.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56087..56746
                     /gene="qseB"
                     /locus_tag="OQM52_000400"
     CDS             56087..56746
                     /gene="qseB"
                     /locus_tag="OQM52_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1562379.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56743..58017
                     /gene="qseC"
                     /locus_tag="OQM52_000401"
     CDS             56743..58017
                     /gene="qseC"
                     /locus_tag="OQM52_000401"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1562380.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58063..58395)
                     /locus_tag="OQM52_000402"
     CDS             complement(58063..58395)
                     /locus_tag="OQM52_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCS1562381.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58714..59295
                     /gene="mdaB"
                     /locus_tag="OQM52_000403"
     CDS             58714..59295
                     /gene="mdaB"
                     /locus_tag="OQM52_000403"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1562382.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59326..59640
                     /locus_tag="OQM52_000404"
     CDS             59326..59640
                     /locus_tag="OQM52_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1562383.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59688..61580)
                     /gene="parE"
                     /locus_tag="OQM52_000405"
     CDS             complement(59688..61580)
                     /gene="parE"
                     /locus_tag="OQM52_000405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1562384.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="OQM52_000406"
     CDS             complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="OQM52_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1562385.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="OQM52_000407"
     CDS             complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="OQM52_000407"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1562386.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63042..63464)
                     /locus_tag="OQM52_000408"
     CDS             complement(63042..63464)
                     /locus_tag="OQM52_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1562387.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="OQM52_000409"
     CDS             complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="OQM52_000409"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1562388.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64299..65780
                     /gene="tolC"
                     /locus_tag="OQM52_000410"
     CDS             64299..65780
                     /gene="tolC"
                     /locus_tag="OQM52_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1562389.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65928..66599
                     /locus_tag="OQM52_000411"
     CDS             65928..66599
                     /locus_tag="OQM52_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1562390.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66605..67765
                     /locus_tag="OQM52_000412"
     CDS             66605..67765
                     /locus_tag="OQM52_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1562391.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDTGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67803..68591)
                     /gene="ygiD"
                     /locus_tag="OQM52_000413"
     CDS             complement(67803..68591)
                     /gene="ygiD"
                     /locus_tag="OQM52_000413"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1562392.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68734..69507
                     /gene="zupT"
                     /locus_tag="OQM52_000414"
     CDS             68734..69507
                     /gene="zupT"
                     /locus_tag="OQM52_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1562393.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69565..69735)
                     /locus_tag="OQM52_000415"
     CDS             complement(69565..69735)
                     /locus_tag="OQM52_000415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562394.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREEAAEKVE"
     gene            complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="OQM52_000416"
     CDS             complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="OQM52_000416"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1562395.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70753..70901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71023..71313
                     /gene="ubiK"
                     /locus_tag="OQM52_000417"
     CDS             71023..71313
                     /gene="ubiK"
                     /locus_tag="OQM52_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1562396.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17833 a        19054 c        17652 g        16936 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaac atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gctggcaaaa atcagactgt gcttcacggt gtagcggaac
      841 agttcagatt ctcggcccac catgcccgtc gcggcgcagg ccacggcgat agattgcggg
      901 gagatcatct tgccagttac gccgccgctg gtatttgctg ccaccagcag agtgtcagag
      961 acgttgattt gctgcgccgt ggtcgattgc agtgaaccaa acagggcgtt tgaagaagtg
     1021 tccgacccgg taaggaatac gcccagccag ccgagaaacg gtgagaaaaa cgggaacatc
     1081 acgcctgtac ctgccagtac cagcgccagc gtggtggaca tgccggaata gttggtgacg
     1141 aaggcgaatg ccagcaccat gccaatcgac aatatcggcc acttcagact aattagcatt
     1201 tcggcaaaga cgccaatccc tttcttgatg cccacgccga ggatgaagat agagataatc
     1261 gccgcaataa aaatagcggt gccgccagcc gagagagggt cgaatttaaa caccgcatcc
     1321 attggcgttg gttgggcgac aatgggtgcc gctttcaaca cttgttgatg caaatgaggg
     1381 atctggaaat taatcaccag tgaataaaac gcgccgcccg gagcaaataa cgctttaaac
     1441 ggcttcatgg tccagatggt gaccagcacc gttaagatta aaaacggtga ccacgcacga
     1501 atgatttgcc ccagactata ttctgaaggc acgggaccgc cagaagatgg cttatttacc
     1561 accatcgcac ctgcggattg ccccatgctg attgccgttt cggtattttt cggccgccag
     1621 actttaagga ataaagcgag tgagacgata ctcaccagcg ccgaagtaat atccggcagt
     1681 tccggaccaa tatagttaga ggtaaagaac tgagtgacag cgaagcttcc cccagcaacc
     1741 agcgccgctg gccacgtctc tttcacccct ttccagccgt ccatcattgc taccagccag
     1801 aacggcacaa gaaccgacag gaacggtaac tgacgtcccg ccattgcgcc aatgtggaac
     1861 ggatcgattc ccgttacctg accggcgacc agaatcggca cgcccaacgc accaaacgcc
     1921 accgtcgcac caaagccagc agctccttcc agcaacgcac caaaggagaa accaatcagt
     1981 aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac
     2041 tgcccgctgg caacggttaa tttatacagg aacaccgccg cgacaataat ccacgcaatt
     2101 ggccataatc cataaataaa gccatagccc gcagcagcaa atgccatatc aatcggcatt
     2161 ttaaaggcga atatcgcaat caggatagat aatataaggg ttattgctcc agcaacatgt
     2221 cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc
     2281 agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg
     2341 ttcagtctct tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg
     2401 actgaactgt aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg
     2461 ctacctctgg cataacaagt atatcgggta agggtttctg ttccgcacac gcaggcgcag
     2521 agtatcgtta agatgtccat attgttgttt taggcccgct agtaatgcgc tacgggtatt
     2581 taatattgtt aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag
     2641 ttactatcat agccccgaca ataaaacttg ccggggcttt tttgacgcta ttaatgactt
     2701 tctttttcgc gtaaacgcca ggcgtgtaat aacggttcgg tataaccgtt tggctgtttc
     2761 acgccgagga agattaaatc gctggcagct ttaaaagcac acgagttagc gaaattcccc
     2821 gccatcggac gataagccgg atcgccagca ttttgctgat caaccacttt cgccatattc
     2881 tccagcgacg cctgcacctg ttctttggtc agaataccgt gacgtaacca gttggcgata
     2941 tgctggctgg agatacgcag cgttgcgcgg tcttccatca gcgccacgtt gtgaatatcc
     3001 ggcacttttg aacaaccaat cccctgctcc acccaacgca ccacgtaccc cagaatcccc
     3061 tgcacgttgt tatccagctc ttgttggatc tcttgcgccg accagttagc gttttcagcc
     3121 accggaatag tcagcagatc gtccagcagc ggttcaaatt cagcactgaa ctcggtctgg
     3181 gcaatgttgg cttgcacgct ctgtacgttg gtatggtggt agtgcagcgc atggagcgta
     3241 gcagcggttg gtgacggaac ccaggctgtg tttgccccgg cacgcagttg gtcgcccttc
     3301 tggctgtaca tgtctgccat caggtccggc attgcccaca tgcctttacc gatttgcgct
     3361 ttaccgcgca gcccacagaa cagaccggaa agcacgttat tacgttcgta ggctttgatc
     3421 cacggcgtcg atttcatctg gtttttacgc agcatcgggc cagcttccat caccgaatgc
     3481 atttcatcgc cggtacggtc gaggaaaccg gtattgatga acgccacgcg gttgcgcgcc
     3541 tgagcgatac agctacgcaa gttcagcgag gtccggcgtt cttcatccat aatgcccatt
     3601 ttcagggtat tcggtgccat accgagcatt gtctcaatgc gggtaaacag tttgttggcg
     3661 aacgccactt cctgcggacc gtgcattttc ggtttcacaa tatagacgct gccagtgcgc
     3721 gagtttttct gcacttttaa atcatagagg gcaatcgcgc cagtcatgac gccatcaaga
     3781 ataccttccg ggatttcatt gccttcgctg tcccaaatca caggaatggt catcaaatga
     3841 cccacgttgc ggataaacag caacgagcgt ccgtgcagag aaatttcaga gccatcggcg
     3901 gcggtgtact gacgatcgtc attcagttta cgtacgattt gccgaccgtt tttctccatt
     3961 ttctcttgca gagtcccctg catcaggccc agcagattgc ggtacagcag gattttatct
     4021 tccgcatcaa ccgccgcgac cgaatcttcg cagtcgagaa tggtactgat agcagcttcg
     4081 acgataacat cgttgatgtg cgccggatcg tctttgccaa tccgcccgtt ggcatcaatt
     4141 tgcagttcaa tatgcaggcc gttatttttc agcaaaatgc aggtcggcgc agcggcatcg
     4201 ccacggtaac cgacaaactg tgctggagta cgtaacgtgg tttctttacc atttttcaac
     4261 tggatgcgta attgtttatc gaccacctta aacgccacca catcctgata gctgccgttt
     4321 tccagcggta gtgattcatc gaggaaacgt cgaacccagg cgataacctg ctcaccgcgc
     4381 tgcggatcgt agccactgac catcgcccct tcctgcggga tgatgtcgct gccgtataac
     4441 gcatcgtaca gtgagcccca gcgagcgttc gccgcgttca gcgcgtagcg ggcgttcatt
     4501 gccggaacca ccagctgtgg ccccgcctga ctggtgattt cgctgtcaat gcccgtggtt
     4561 tccaccgtca cgcgctccgg ttgcggcacc aggtagccca gttcacgaag gaaagattta
     4621 taggccgctt tatcttttac cggccccgga ttgctgcgat gccactcatc aagcgccgcc
     4681 tgaatgcgat cgcgttctgc cagcaactga cgattttctg gtgccagatc gtgaacgatc
     4741 tcatcaacat tgtgccagaa tgcagcagcg tccagttcca cgcccggcaa aacttctcca
     4801 tccacaaaac gtttaaaatt ggcgtcaatg cgtaaacggc cctgggttat ggtttgactc
     4861 attgtttctc tcctcgtttt cgcttatttc gccaacaccg ctgctgccac tttcgcgacc
     4921 tgcgcatcct gtgctccggt taaaccagaa acgcccacgg caccaataat ttgcccatca
     4981 acaacaaccg gtacgccgcc ttccagcgac gtcagtaacg gcgcagtcac gaacgcggta
     5041 cgtccgttgt tcaccatctc ttcatagccc ttagtttcac gacgccccag cgcggcggta
     5101 cgcgctttct cctgggagat gtaagccgca atcggcgcgc aatcgtccat gcgacttaac
     5161 gccagcagat gaccgccgtc atcggcaaca gcaatggaaa cagaccagtt atttttctgc
     5221 gcctcttcct gacctgcggc aataattgca ctcgccattt gctggctaag aatgacttta
     5281 gttttcattt ttttattcct tttcaagggc ttgttctaca atttcaatcc agtgacgcac
     5341 agaggtacga ccggcgctcg ccagatgcgt ctggcaacca atgttggcgg tgacgatcat
     5401 ttccggtttg ccgctttcca gcgcattcat tttgttatcc cgcagctggc gtgccagatc
     5461 gggatgcgtt aacgcatatg ttcccgctga accgcagcac agatggctgt cgggaacgtc
     5521 cgttaaggta aatccaagac gaagtaacac tttttccact tcgccgttca gcttttgcgc
     5581 atgttgtagg gtacacgggc agtggaaggc cagtttttta tcgccgcgaa ttgccagttt
     5641 ttccagcggt tcctcgcgca gaagttcgac taaatcgacc gccagttcac tgacctgacg
     5701 cgctttatcg gcatataacg catcgttttt cagcatctgc ccatactctt tgacgaacgc
     5761 gccgcaaccg ctggcggttt gcaaaattgc ctcggcacct gcttcaatcg cgggccacca
     5821 ggcatcaata ttattgcgcg cccgtgccaa ccctttctcc tgcgcattaa gatgatagtc
     5881 caccgcgcca caacagcctg cttcgttagc tgacatgacg ctgatcccca gacgatccag
     5941 cactcgcgca gttgccgcgt tggtgttggg cgaaagcgta ggctgggcgc agccttccag
     6001 cattaaaacc cgacgcttat ggcgcagcgg cggacgcggt ttagctttca ccgtttcagc
     6061 aggcagtttt gctctgacct gttccggtaa aaacggtcgc agcaccagcc ctacctgcgt
     6121 cagcgcacgg aagaccgccg gacgcggcac tacctggcgc aatccttcgc gcagcattcg
     6181 ctccggcagt gggcgtttca ctttctgctc gacaatatca cgcccgatat ccagcaaatt
     6241 gtgatagcgc acaccagaag gacaggtggt ttcacaattg cggcaagtga ggcagcgatc
     6301 gagatgctcc tgtgttttaa gcgtgacttc gttgccttcc agcacctgtt taatcagata
     6361 gatgcgcccg cgcggcccgt ccagttcatc gcccagaagc tgataggttg ggcaggttgc
     6421 ggtacaaaat ccgcagtgaa cacaggcgca caggatgctg tcggcttcca gcgcgcgttc
     6481 tgccgcatct cttcggttaa tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat
     6541 gcgaccgggg ttaaacacgc cgcaagggtc gagctgctgt ttaagctgct ggtgatagcg
     6601 gaataaagga gccgatagcg gggcaaagcc accatctccg gcactaaagc gggtcgcatg
     6661 accgccagcg ttgcgggcga tgcgatggat ttgattgtca tcggctgtcg atttcagcca
     6721 gcgtaacgcc ccgccccagt cgatcagttg ctcgccgggt aaatccatca tcggcgcatc
     6781 gctgggtaat gaaatgcgcc ataaggtacc tggtaacgag aagaacggca gttgttgttc
     6841 acgcaattgc tgccagaact gacccacaac ctcttcgcca cccagcagtt cacgcgctgc
     6901 ttttaccgat ccttcgccgc cctcaaggcg gatccacaac gcattgtcga agtaacataa
     6961 gccactaatg ggtaatggct ggagttgcca ctcggcgatt tcactcatgg cttcttgcag
     7021 gctgatttcc cgacgcaggc tcagagaggc acgcggtcgc ggtaacactt tcattgagat
     7081 ttcagtgagc acgccaagac aaccgtagct tccggccatt aaccgtgaga gatcgtatcc
     7141 ggcaacgttt ttcatcactt cgccaccaaa acgcagatgt tttccagtgc cggtaatgat
     7201 gcgcgtgccg aggacaaaat cgcggaccga accgctccac gggcgacgcg gccccgccag
     7261 cccgcaggcg accatcccgc cccaggtggc ttcttcacca taatgcggcg gctcgcaggg
     7321 aagcatttgc ccggcgctct ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg
     7381 agcggttatc accagctcgg tcgggtcgta attaacaatg ccgcgatgac aacgcacatc
     7441 cagcgtttgc ccggtgactg ggcgacctaa aaaggctttg ctattgctgc cctgaatcac
     7501 cagcggcgtt ttatcgctaa ttgcctgatt cacctgctcc agcagcgcct gactgtaatc
     7561 acactcgcgt agcatcaaaa gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca
     7621 tgcatggcac caaattcagc acagcggtgt agcgtgggaa tgtttttccc agggttcagc
     7681 aaaccatcgg ggtcaaacgc cgccttgacc gcatggaagg tcgtgatttc atcgctgttg
     7741 aactgggcgc acatttgatt gattttttct cgcccgatgc catgttcccc actgatgctg
     7801 ccgccaactt caacgcagag ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat
     7861 tcaccaggtt cgttggcatc gaaaaggatt aacgggtgca tgttgccatc tccggcatga
     7921 aagacgttgg caacacgtaa atcatattgc tgcgataaac gggcaatgcc ttccagtacg
     7981 ccaggcaggg cgcgacgcgg gatggtgcca tccatgcagt agtaatccgg ggagatacgt
     8041 cctaccgccg ggaacgcatt tttgcgaccg gcccagaaac gtacgcgctc tgcttcgtcc
     8101 tgtgccaaac ggacgtcagt cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag
     8161 tcttcctgta cgtcagactc cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg
     8221 acgggataac cggcatgaat aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc
     8281 tccagcccgc cggggataat gccattggcg atgatgtcac caaccgcaag tccggctttt
     8341 tctaccgagt caaagctggc taacagaacc cgcgccacgg gcggcttcgg cagcagtttt
     8401 accgtcactt cggtggtcac gccgagcata ccttccgatc cggtgaacag cgccagcagg
     8461 tcaaaaccag gtgaatccag cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt
     8521 tgcacttcaa ttttcagcag gttatgtacg gtcagaccat atttcaggca gtggacgcca
     8581 ccggcatttt ccgccacatt gccgccaatt gaacaggcga tttgtgagga aggatccggc
     8641 gcgtaataga gattatgcgg cgcaacggcc taggagatcg ccaggttaca tacgcccggc
     8701 tgcacgcgcg cgcggcgacc aacggggtta atgtcgaggc tctctttaaa gcgcgccatc
     8761 accaacaaca cacctttttc cagcggcagc gcgccaccag aaagcccggt gcctgcacca
     8821 cgggtcacca ccggtacacg caggcgatgg cagacagcca gaatcgctgt cacctgttcc
     8881 atttgcttag gcagaacaac cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac
     8941 tcgtaaggaa tgatctcctc atcggtatgc aggatctcaa gtccagggac atgctcacgc
     9001 agtgccatca gtaccgatgt gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg
     9061 tacaagatgc tcatgagtag gcttcgcttt gttgtgttgt gtggcagctg atttttgcgc
     9121 gctgcttctg tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc
     9181 ggtcagacca ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg
     9241 atgtaacata atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt
     9301 cctacctgtg cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt
     9361 gcagagagta tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc
     9421 tcggaacgtc gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg
     9481 accgtgctgc gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca
     9541 cggcttaatc gggagcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga
     9601 acgctgtacg atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg
     9661 gcaacgctgg gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc
     9721 gccgccagtg agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct
     9781 ttccatctcg ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca
     9841 ttgaccgatc tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca
     9901 cagcaaaaaa agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg
     9961 ctgccgcacg tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc
    10021 cacgatatcg agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac
    10081 aaggtgggaa tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac
    10141 ttaacaggat ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc
    10201 gctaaacagg atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc
    10261 cgccccaatg cgttccagca tgttattcat gcctgtattg gtttcgagga tccatgacat
    10321 ctccagcgca tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag
    10381 cgcaatcacc gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac
    10441 tcgcgcagtt cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg
    10501 aaagagcgat ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cacaataaac
    10561 gcgcagggtg cagaatcaat ctcagcgatg tagatcatat cgtccggcac cagatattta
    10621 agttggtcgc ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc
    10681 tgccagccag agttgaaaat ctcacgcagg atctgcatct cttcggcaaa ccgctgacga
    10741 ttgatgcagc gaatggtcac ctttttgcgc acctgatcca tcagtttttt tagcgccgga
    10801 gagaaagtga gatcggttcg ctgcatccac cacgccagta aatcaatgcc tttgtgataa
    10861 cccagttgtt caatatgcgc ggcataccac ggtttgccgt gtggcatcat cgcacagggt
    10921 ggtgtgtcaa aaccttcaat cagtaatccg ctttcctgat tgatattcag gctgaaaggc
    10981 ccgctgatct tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg
    11041 gcaaaatcct gcggatcatc aatggcgtca atcatgccga aatgaccggt atcttcaccg
    11101 taacgctcac ggtgcaaggt atctatttgc gcggtaatac gcccaactat ctgccccgct
    11161 ttttttgcca cccacgcctg ccagatgata tggtccgtcc ccggattttt cgcagacaaa
    11221 tgctcgttgc gttcaatgaa taaagggggt atccagtttg gatcgtcggg ataaagtgat
    11281 gacggaaaag cgataaatgc cttaaggtca tttttattaa ttactttttc aattttaatc
    11341 atcagcagat cgcattccat atctctgtgg ataacaaata attaatggtg aaaaacaggt
    11401 tgcaacagca acgagcggca tcttaataat caactcaatg aatacaaacg acgaataaat
    11461 agtattaatt aatgattatg ataaggtcag aggctacaca gaaacgcctc aatgcctttt
    11521 gtcaatttaa ggcgaccgga caaggcttct tcttaatata ttcataaatt aaatatcatt
    11581 cataataaaa acaaaaaact taaactcatt atttaaatca atgttcattt tcacaccgat
    11641 gaaaatacta tctcatttat cttttatgcc tatataatcc accgcattat tgtcatttta
    11701 tttttgatat ccaatttttt cgtggtgtat atgtctaata aaatctttac gcattcccta
    11761 cctatgcgct atgccgattt tccaacgctg gttgatgctt tggactacgc cgctctgagt
    11821 agagccggaa tgaattttta tgacagacgt tgccaacttg aagatcaact ggaatatcag
    11881 acattaaaaa cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa
    11941 ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc
    12001 tgtcagtatg ctggcttagt cgccgtcccg ttggcgattc caatgggcgt cggtcagcgg
    12061 gattcctgga gcgccaaact ccagggttta ctggcaagtt gccagcccgc agccattatc
    12121 actggtgatg agtggttgcc actggtcaat gccgcgacgc ataacaaccc cgaattacat
    12181 gttttaagcc acgcttggtt taaggcatta ccggaagccg atgttgcgct ccagcgtcca
    12241 gttccgaacg atatcgccta cctccagtac acctccggca gcacccgttt tccccgtggc
    12301 gtcattatca cccatcgcga agtgatggct aatctacgtg ctataagcca cgatggcatt
    12361 aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga tatgggactg
    12421 gtcggctttc tcctgccccc cgtcgccacg caactttcag tagattattt gcgcactcag
    12481 gattttgcca tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt
    12541 tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc
    12601 gctgaactgg atctttcctg ctggcgcgtc gctggtattg gtgctgagcc gatctccgca
    12661 gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta actttgacaa taaaactttc
    12721 atgccgtgct acggactggc agaaaatgcg ctggctgtca gcttctctga tgaagcctcc
    12781 ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg
    12841 ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa
    12901 catggtattg aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat
    12961 atttgcatct ccggtcccag tctgatgagc ggttactttg gcgaccaggt ttcgcaagac
    13021 gagattgccg cgacgggctg gttagacacc ggcgacctcg gttatctgct ggacggttat
    13081 ctgtatgtca ccggacgcat taaagatctg attattattc gtggccgtaa tatctggccg
    13141 caggatattg aatatatagc ggaacaggaa ccggaaattc attctggcga tgcgattgct
    13201 tttgttaccg cccaggaaaa aatcattttg cagatccagt gtcggatcag cgacgaagaa
    13261 cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc
    13321 gcggctatcg atctgttgcc gccccacagt attccccgaa cgtcctccgg caagcctgcc
    13381 cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa
    13441 tccctggcat aaatcaaact gtcgcggtga cgggcgctac cgggtttatc ggtaaatata
    13501 ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt actgcccgcg
    13561 ctcacgtcaa tgataatctc acctgggtgc gcggttcgct ggaagatact cattcactta
    13621 gcgagctggt tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca
    13681 aagaagagat tttcacccgc tgtaacgttg acggcagcct gcgcctgatg caagcagcaa
    13741 aagagagcgg cttttgccaa cgttttctgt ttatctcttc gctggcggcg cgccatcccg
    13801 agctctcctg gtatgcaaaa tccaaatacg tcgccgagca acgactggca gcaatggcgg
    13861 acgaaattac gctgggcgtt tttcgcccga cagccgtgta tggtcccggc gataaagagt
    13921 taaaaccgct gtttgactgg atgctacgcg gcctgctgcc acgacttggt gcgccagata
    13981 cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg
    14041 ccgaaactgt acagacgcaa acctatgaat tatgcgatgg cgtcgccggt ggctatgact
    14101 ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg ttccgtgcga atggttgaca
    14161 ttcctctgcc ggtactcaca ggccttgcgg atatcagtac cgcgttgagt cgcctggcag
    14221 gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg
    14281 caagtaataa ccgtatttct gaagatatca attggtttcc cgggattagc ctggaacacg
    14341 cattacgcaa cgggctattt tgattgtctt gttttaaagg tgtgttaacg tttctatggt
    14401 aaatcgtgaa atagtaatgg attacatcct ttcctgttta caggatttgg tcgaaaacgg
    14461 tgtggaaatt aagcccgaca gcgatctcgt caacgatctt ggcctggagt cgatcaaagt
    14521 gatggatctc ttaatgatgc tggaagacag attcgatatt tccattccta ttaatattct
    14581 gctggatgtc aaaacccctg cgcaattaat ggaaactcta cttccatggc tggagaataa
    14641 ataatggggc tatacgataa atatgcgcgc cttgctggcg agcgtctgca atttagtgac
    14701 aacgggttaa caccgtttgg cacctgtatt gatgaagtgt attccgccac cgaaggccgg
    14761 atcggcaata aaaaagtgat tctggccggg accaataatt atctcggttt aacctttaat
    14821 catgatgcca tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg caccaccggt
    14881 tctcgtatgg caaacggcag ttatgcgccg cacctggcgt tagagaaaga gattgccgag
    14941 tttttcaatc gccctaccgc tatcgttttt caaccggtta taccgctaat ttaggcgtca
    15001 tcagcgcact tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta
    15061 tttatgacgt ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta
    15121 aagatctgga acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg
    15181 ttgagggcat ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca
    15241 aacgtcgtct cggtggttat ttgattgtcg atgaagcgca ttcgtttggc gtgttaggcg
    15301 caacggggcg cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg
    15361 gtactttcag taaaagtctg gcctcgattg gcggttttgc tgtcgtttcg gaagcgatgg
    15421 aagtgctgcg ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca
    15481 ttgcgaccgt gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc
    15541 tgatggataa cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt
    15601 cacatattag cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct
    15661 ggcgcgagct tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg
    15721 caggcatcac gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg
    15781 ccatcattca ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc
    15841 cacggcgggt ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa
    15901 acgctaccgg taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat
    15961 taaccatgat ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac
    16021 ggctacccat cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca
    16081 tcgccagggt caggatgggt tgccccagtt tttcccacag tgcaaggtgg tattcgctgc
    16141 tgggttgccc ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag
    16201 tattccccgg cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa
    16261 gattatctaa tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat
    16321 gcaggaccca cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa
    16381 gcgagagatc gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca
    16441 gcgatttcac cgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt
    16501 catcgtcctc ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg
    16561 gcgacgccac ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca
    16621 gcgccaccag tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac
    16681 tgtttttcga taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa
    16741 gttcgaccag tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat
    16801 aaccgcccgg gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca
    16861 gcaatagccc tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga
    16921 aaacattcat cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag
    16981 gcccattagc atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt
    17041 agccaccagt gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag
    17101 tggcaataac gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag
    17161 cagcgccatc aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc
    17221 ggcaggaaag gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg
    17281 gctttgatcc agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc
    17341 acgcccctga tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc
    17401 gggtgatggg tcaaccacat caaccgaacc ggcgcggaaa atacgggttc gattggcagt
    17461 agaagtataa atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag
    17521 gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag
    17581 ctccgactgc gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata
    17641 catcgacaga agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta
    17701 caagcgtccc ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat
    17761 ctgccctagc gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc
    17821 aatcagtact ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc
    17881 cgcttcggtc agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac
    17941 gataatcagc accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc
    18001 actaacgccc tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat
    18061 gacatgatct acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc
    18121 tttattcagc accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg
    18181 tccattgccg tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt
    18241 gccaacaaca aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg
    18301 ctgaaaacga tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt
    18361 tttcttcgcc gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg
    18421 tgtgctgttc cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc
    18481 ttcctggata taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc
    18541 cggggctaag agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc
    18601 tatagcggaa aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc
    18661 aacggtgcat aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta
    18721 tctcccatca ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt
    18781 tcaggatcaa gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc
    18841 acatcatgaa attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc
    18901 atttgctttt gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat
    18961 gaatagttca ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa
    19021 tacgacacaa tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg
    19081 gtcgttttgc cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa
    19141 cgcaggtgct tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc
    19201 tcatcgcctt tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg
    19261 agccagaatt gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt
    19321 gcaatagtcg gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca
    19381 aatagacgct cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg
    19441 ctgcgttata aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga
    19501 gcgccacgtc cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca
    19561 gatgacagaa catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga
    19621 gaaaagcgcg ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg
    19681 aaatcacaat tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc
    19741 caaacaccag cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac
    19801 cgattccgcc cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg
    19861 gatctaaacg catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg
    19921 cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga
    19981 tccccaccaa cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg
    20041 ttgaccagac aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt
    20101 tgaaggcgtt atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg
    20161 tgccgccaac cagattcgac acgtaccagg caataacaaa acccacaaaa ccaaagtcaa
    20221 aataccaggc tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag
    20281 caatcaaatc aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg
    20341 aaggaatgag cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg
    20401 aatgggaaag gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga
    20461 tatccagcga gaaggaaaat gagacgacat tgcgaaattg ctgcggatta ttgttggtca
    20521 aggctggcgt tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga
    20581 tcgacttggc gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt
    20641 taccggcaca cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta
    20701 accaggcgct attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt
    20761 taaaacccgc caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt
    20821 aagttaaact tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt
    20881 ccagcacttc gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc
    20941 caatcgtcga catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc
    21001 gggagatggc agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg
    21061 ccattttttg gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg
    21121 cacgttcgac gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga
    21181 agcgatcgct gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg
    21241 ccattcttcg cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg
    21301 ttttcatgct ttgggttttc gaggatttgg ccgccaatcg ccgttccagc cagacgccaa
    21361 ccaacggcaa tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc
    21421 gctcggtttg ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac
    21481 cggtgccatc acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg
    21541 ttgtactatt aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat
    21601 tttggtgatc aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg
    21661 gctggttgcc gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta
    21721 acggcgattt tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt
    21781 tgttcatcaa tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac
    21841 agcttcaact cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc
    21901 aattgcgggc catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg
    21961 agcagatagc cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag
    22021 gaaagcagat agataaccag cgcagtaata ttgccaggcg gagttaaggg cttttcgtgt
    22081 acatgtgccg ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt
    22141 gaaatccatt cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt
    22201 cttgctgcca gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat
    22261 cctacaatgg caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc
    22321 acgaaaaacc cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc
    22381 gttcaacctt attgtgacaa tgactaattg tgacattgac ctgaattacg ctttcataaa
    22441 aacatattaa ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca
    22501 gtcaataaca cctccattaa ttgccgttat tggtagcgat gcttcaggca agtcaacggt
    22561 gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg
    22621 aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt
    22681 acataaaaca attgaacgaa atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc
    22741 ggcgctggta attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc
    22801 ctgtcgtcgt cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg
    22861 cgcttacgat ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct
    22921 ggcaagccag gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat
    22981 caaactcaat gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc
    23041 gctggcgagg aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga
    23101 tatcgatgcc aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga
    23161 ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg
    23221 ggcattttca ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa
    23281 aaattgccgc cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct
    23341 gtaacccacc gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg
    23401 tggagacagg catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg
    23461 ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg
    23521 ctacacgacg ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga
    23581 tccacaccgg agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt
    23641 tctggtcttc tttactgacg cctggcagtt tcgccagctt cgcggaggta tcggagatgc
    23701 acagcataat gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca
    23761 tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac
    23821 agtgaaactc tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg
    23881 caatcaactt ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg
    23941 catcgcgggt acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag
    24001 gggcaatccc caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcgcgc
    24061 cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg
    24121 gtttacgttt gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct
    24181 ttttcgtccc actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc
    24241 cgacgatagg ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg
    24301 catccatcac cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac
    24361 caatcaaggt gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga
    24421 taatcgccgc cagcagcatg gaaaagacca tcgccaaacc gtgagttgac cccatattca
    24481 gcaacaaatc gattggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata
    24541 acacgccaaa aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac
    24601 gagtataaat aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt
    24661 agaacaacac aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa
    24721 caaataaatt tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc
    24781 agtgacttta acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta
    24841 tttcttgtgt atcagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag
    24901 gtattccact ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat
    24961 tgaatatttt gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca
    25021 acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca
    25081 gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata
    25141 cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct
    25201 gctcggcaaa tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga
    25261 tattgctacc acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat
    25321 cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga
    25381 tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt
    25441 ccgggcgcag caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg
    25501 gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg
    25561 cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag
    25621 catcccagcc cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca
    25681 gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat
    25741 ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt
    25801 ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc
    25861 ccgcttcggt atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct
    25921 catccatgca gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga
    25981 gacgcaaacg tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt
    26041 tcagcacctt gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta
    26101 gctcctgctc ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct
    26161 gaccgttggc ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt
    26221 caaactggcc tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt
    26281 ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg
    26341 tgtcatcaaa agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc
    26401 gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga
    26461 ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc
    26521 ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg
    26581 cctgcaatgg caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct
    26641 cccacgccat acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag
    26701 caaattcaac gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata
    26761 cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta
    26821 ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt
    26881 tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg
    26941 attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag
    27001 cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc
    27061 ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa
    27121 agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc
    27181 tggtccgacg catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct
    27241 gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt
    27301 tactggtgca gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag
    27361 cggctttgtc gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa
    27421 tccgccgatc cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg
    27481 ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt
    27541 acagggtgcc gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt
    27601 gaaaattgag tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct
    27661 ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat
    27721 ggcgatttgg ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt
    27781 tctggatgcg ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc
    27841 ggcagtgaaa cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca
    27901 tgagcgccat gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg
    27961 gttggtgttc gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca
    28021 atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca
    28081 tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc
    28141 atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag
    28201 atcggcaggg ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac
    28261 ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc
    28321 aatacacatt gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta
    28381 gcgggtgttc cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc
    28441 tttttaggcg ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc
    28501 cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa
    28561 tctccaccac ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta
    28621 aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc
    28681 tctcctcaac gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat
    28741 cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat
    28801 gcattttcgc ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc
    28861 ggattggtga ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct
    28921 tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa
    28981 acaccgtcca gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt
    29041 ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc
    29101 agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa
    29161 acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt
    29221 tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca
    29281 gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac
    29341 ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc
    29401 cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg
    29461 cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa
    29521 cgccggaact tcttcatccc gcaggatcat catcgttccc ggagtgttct ttttcgacac
    29581 aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc
    29641 cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc
    29701 ttcgacaatc cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac
    29761 taaaatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg
    29821 tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt
    29881 ttattcggat cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca
    29941 ttgaagttac gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg
    30001 ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca
    30061 acacctttga actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct
    30121 ttgccgatat tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag
    30181 cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc
    30241 gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat
    30301 tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg
    30361 ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa
    30421 gccggaatgg tggtaccttc ccacggcgcc tgtggcgctt cgtctttata ccaggagtgc
    30481 ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga
    30541 tacgaggaca gatccgcatt ctcaatgtag cctcccggga acaggaagct gccgtttttg
    30601 ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc
    30661 cattccgggt agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag
    30721 tcgctcagtt tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc
    30781 aacccgtcga ggttgattgg gttcgcgaca ccacccacag ccaggttctg aatgtgcggc
    30841 gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc
    30901 aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc
    30961 cagtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc
    31021 tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt
    31081 tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc
    31141 gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta
    31201 cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg
    31261 gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca
    31321 tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc
    31381 catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata
    31441 cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat
    31501 gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc
    31561 aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg
    31621 cgtaaccgcc gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac
    31681 ctaacagtgc gctcagtgct gacaggaaca gcatacggga atcattacgc agcttcgcca
    31741 cacgcagaac gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt
    31801 agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca
    31861 gcccgatgaa aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct
    31921 tttcatccgg accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa
    31981 tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct
    32041 gccagagcgg atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt
    32101 gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt
    32161 gtagcgacac cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca
    32221 cgccgatgta gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt
    32281 gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca
    32341 agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt
    32401 attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc
    32461 cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt
    32521 cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga
    32581 ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat
    32641 catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc
    32701 acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga
    32761 atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca
    32821 taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga
    32881 tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc
    32941 ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc
    33001 tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga
    33061 ccgcctttat cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca
    33121 ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa
    33181 cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc
    33241 agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc
    33301 ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac
    33361 acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc
    33421 ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag
    33481 cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt
    33541 tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct
    33601 gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct
    33661 tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca
    33721 caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat
    33781 ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat
    33841 ggaagccgat acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca
    33901 caccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac
    33961 agccgaggtg gtacaagcac cagccttcgc ggtgtccttc atcaccgaac tctttggcaa
    34021 aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg
    34081 cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg
    34141 caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg ataacggttt
    34201 tgcctggcag aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa
    34261 caccgcccca cgcggagcag gaaccaatag cgataatggc tgctgagcct tccgccgctt
    34321 tgcggatgtg atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta
    34381 atgggatgga accatccacc actaacacat actgcccttt gtacttctcg agagcgttat
    34441 gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag
    34501 agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg
    34561 tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa
    34621 ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg
    34681 cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc
    34741 cagtcattta tagttattcc gttgcgagaa cctggcatat attttgcctc aatcgcaaaa
    34801 tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat
    34861 actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat
    34921 aatacgtatg tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga
    34981 acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg
    35041 cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatcct
    35101 gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac
    35161 cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt
    35221 catcgttcac ctcattcatt atatatcgcc atatataacg atattttagg caaagaacga
    35281 cttttgcgca cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct
    35341 atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag
    35401 atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat
    35461 aagcctcata aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt
    35521 tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat
    35581 cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga
    35641 cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg
    35701 tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg
    35761 ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa
    35821 cggaacttaa tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca
    35881 catcttcgat atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt
    35941 tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg
    36001 aaggatacgt gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca
    36061 gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat
    36121 acttcgccgc aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt
    36181 agtaatccaa caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc
    36241 tcaactggtg tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact
    36301 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
    36361 aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
    36421 gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcattat cgctcggttt
    36481 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc
    36541 gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
    36601 cgcagaagag aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact
    36661 gattatctct accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc
    36721 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
    36781 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
    36841 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
    36901 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
    36961 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
    37021 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
    37081 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
    37141 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
    37201 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
    37261 tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt
    37321 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
    37381 cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg
    37441 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
    37501 ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt
    37561 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
    37621 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccacttca
    37681 gcttctcttt gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca
    37741 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
    37801 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
    37861 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
    37921 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
    37981 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
    38041 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
    38101 atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt
    38161 taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
    38221 caggcatcca ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta
    38281 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
    38341 ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
    38401 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
    38461 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
    38521 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
    38581 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
    38641 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt gtcatcacca
    38701 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
    38761 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
    38821 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
    38881 aggataagat cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg
    38941 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
    39001 tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
    39061 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca
    39121 aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc
    39181 aggcattgag cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac
    39241 gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat
    39301 tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc
    39361 ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg
    39421 gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt
    39481 ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata
    39541 aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac
    39601 atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc
    39661 gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag
    39721 atgcattgcc atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc
    39781 agcggcgctg gcttccagat ctaggtcacg gctttgcagc aacaatacct gcgctgcaac
    39841 atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac
    39901 cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg
    39961 cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat
    40021 gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata
    40081 gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct
    40141 ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg
    40201 cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt
    40261 ggcctggttt aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt
    40321 ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta cagaggccaa
    40381 aataagccca atcatcacgc acttaacgac aatatcggcg tactgataca taccccagac
    40441 ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa
    40501 aatctgcgca taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg
    40561 catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca
    40621 cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc
    40681 atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc
    40741 ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata
    40801 gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga
    40861 cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt
    40921 ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg
    40981 gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga
    41041 tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg
    41101 gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc
    41161 caaacactaa aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg
    41221 ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca
    41281 acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc
    41341 aggccgccac caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca
    41401 atgcccgttg ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg
    41461 ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac
    41521 atcatgaaag cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgcgcgag
    41581 ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag
    41641 gcagcagcgg gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga
    41701 actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt
    41761 tgatcctggc aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt
    41821 ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg
    41881 atctggacgc cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga
    41941 cattttattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc
    42001 aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt
    42061 attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat
    42121 attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg
    42181 cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa
    42241 ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc
    42301 tggctaagtt atattcaggg gagctggtta gggaatacca aaacggtgaa aggctggctg
    42361 gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg
    42421 ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg
    42481 ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg
    42541 tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc
    42601 catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc
    42661 ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc
    42721 ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc
    42781 cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc
    42841 gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat
    42901 catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac
    42961 agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc
    43021 gacgctcagg ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact
    43081 gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac
    43141 gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc
    43201 catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt
    43261 aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag
    43321 ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt
    43381 ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc
    43441 ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat
    43501 gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac
    43561 atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc
    43621 cagcaagcga caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca
    43681 atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat
    43741 tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag
    43801 atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct
    43861 gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat
    43921 tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt
    43981 tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga
    44041 gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt
    44101 gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc
    44161 agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga
    44221 gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc
    44281 caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt tccattctgc
    44341 acatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg
    44401 tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa
    44461 accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctaatcga
    44521 agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg
    44581 ggcggcgact caggcgctga acggtttgat tggcactggc gtaccgcagg actgggcaac
    44641 gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc
    44701 tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta agctgctgca
    44761 atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc
    44821 gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta
    44881 cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac
    44941 ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc
    45001 ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc
    45061 acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat
    45121 ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg
    45181 caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt gggttatcgc
    45241 tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat
    45301 gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag
    45361 cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc
    45421 tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg
    45481 atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat
    45541 cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt
    45601 catccgctga tgcaacaacg ccagctacac gcttggaacg cgacacacaa aatccagacc
    45661 gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc
    45721 gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat
    45781 agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat
    45841 gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc
    45901 aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc tcccggtggc
    45961 ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt
    46021 gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc
    46081 tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat
    46141 gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc
    46201 ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag
    46261 tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc
    46321 gcttaccctg aacgacaatc ttacactacc gggcaatatt ggctggctgg attatgatcc
    46381 ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga taacccaaac
    46441 tgacgtcgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa
    46501 gaccacacag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg atgatattgt
    46561 cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc
    46621 tttagcttcg ttgcagaaag aacagccaga atcctttgtt gctgaactct ttgaacatat
    46681 tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa
    46741 tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga
    46801 acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt
    46861 tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac
    46921 gctgaccaat ggcaacaatg aagagcaaga gctggtttta cagcggcgcg aaggtaagtg
    46981 ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa
    47041 aaccgccgcc agattaaagc aatgagctga attaaataac aattagccgg aacaataaat
    47101 aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc
    47161 ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca
    47221 ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat
    47281 gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat
    47341 tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt
    47401 tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg caccagcatt
    47461 caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct gacgctgagt
    47521 cgccatcggc gtatgtttag tcaacgccgg acgggtattg cggttctggc gacgagcttc
    47581 acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc caatcagatg
    47641 ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg gatcgtggta
    47701 acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga agacgccttc
    47761 gctcttataa ccaatcttcg ccagcggttt tttcccggtg taatacatgg tggttgagtt
    47821 cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat gctttttcag
    47881 ccacagcgcc aaattcacca tatcttcatc acgcgtaccg gggtgcgcag agatgaaata
    47941 cgggatcaga tactgctctt tacctgcctg tttcgagtag gtatcgaaca gctctttaaa
    48001 gcggtcatag ctgcccatgc ccggcttcat catcttcgac aacggccctt cttcggtatg
    48061 ttccggagca atcttcagat aaccgccgac gtgatgggtc gccaactctt tgatatagcg
    48121 cggatcttcc acggcgatgt cataacgtac gcctgaggcg ataaggattt ttttaatgcc
    48181 tttcagatca cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag tgtccatgtg
    48241 cggacaaata tccggataaa cgcacgacaa acggcgacaa gtttgttcag cgcgtggcga
    48301 tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa tcacgcccgt
    48361 aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat cttccgaacg
    48421 gctctgaata atgcgccctt cgtgctcggt aatagaacag aaagaacagc cgccaaagca
    48481 gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac gggcattgcc
    48541 ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca tctcttcggt
    48601 agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt tttgcatcaa
    48661 tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat gggcgtacag
    48721 cactttatcg cccttcactt tctcgaaaga aggcagcaat acgtaggttt tttcccacgg
    48781 tttctggcgc ggtggctgca cgattacagc tttcgcttcc tgctttttcg gcgcaacagg
    48841 tttgttatcc gcacacggca aatcttcgcc atacggatgc gggattgggt cgattttccc
    48901 tagggtatca agacgggtgg aatccacacc gctccagcca ggcagcgcct cttttacgat
    48961 aattgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg ccagacgatg
    49021 cgctacttcc accagcggac gttcaccgtt accaaacatc agcatgtcgg ctttcgaatc
    49081 taccagaacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg tacggcgcag
    49141 gctagcctca ataccgccga ggatcaccgg cacatctttc cacgcctctt tacaacgctg
    49201 ggtataaacc agcgtggcgc gatccggtcg cttacccgcg acattatctg gcgtgtaggc
    49261 atcatcatga cgtaaacggc gatcggcggt atagcggttg atcatcgaat ccatgttgcc
    49321 agcagtaaca ccaaaaaaca gattcggttt acccagacgc ataaagtcgt ctttgctgct
    49381 ccagtccggc tgggcgatga tcccgacgcg aaagccctgc gcttccagca tacgaccgca
    49441 aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc
    49501 gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa acggtgccgg
    49561 tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg gttggatcag
    49621 ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg ccggggatta
    49681 taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc gggcgagtat
    49741 gaagattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc cgccatttcc
    49801 agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca
    49861 agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac
    49921 ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg gaacgcttgc
    49981 ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt gacatcaata
    50041 cggttgacgt cccacagctg tccattaata cccgggtcat cacccagact gatatcgcga
    50101 ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg aagactgtct
    50161 gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccaaggtaga aaccagaata
    50221 ctggatggct caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc accacaggtg
    50281 atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc gcccggtgcc
    50341 agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc agaaatcaca
    50401 tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga cgcgttcagc
    50461 agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc
    50521 agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag
    50581 ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata atggttgggg
    50641 ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag gccgttatag
    50701 acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg
    50761 cgaataggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc
    50821 atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg
    50881 ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa
    50941 cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt
    51001 tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc
    51061 ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca aagtgcaatc
    51121 cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc
    51181 taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt
    51241 ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta
    51301 tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga ctgacgtcaa
    51361 ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga ttaatcttat
    51421 tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa
    51481 atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct tccgggaaca
    51541 tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg gtgccgtgag
    51601 ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga
    51661 agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt
    51721 tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata gcattgccgt
    51781 agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagt ggcgcaagac
    51841 ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt
    51901 aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga
    51961 tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca cctgaaatca
    52021 ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga
    52081 ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg ctgccggatg
    52141 acgacttaac gtttcatccg gcgttccttg ctagcgggag gaaacagcgc cctccccggc
    52201 atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct caacacgatc
    52261 gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag tgactttctg
    52321 tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca gcgtgctttg
    52381 cggcggcaga acgtacagtt gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg
    52441 aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa
    52501 catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat cttcaatcac
    52561 caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac ccgcacggtt
    52621 acgcgctacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag
    52681 cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag gcggcaacgt
    52741 taatttgccg gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat
    52801 ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttactct tacctttcac
    52861 cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga tatcatggcc
    52921 tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt cagacggcag
    52981 catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc
    53041 gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat tcattttacg
    53101 ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac tctgctcacc
    53161 gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga ggatcgcttc
    53221 cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt catcttcgtt
    53281 acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac cttccaggat
    53341 atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca cggtatcgcg
    53401 acggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg gacgaccatc
    53461 cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg tagcgaagag
    53521 gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca ccagacgggt
    53581 cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct ttttgttgcg
    53641 catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg gtaatgcgct
    53701 gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg aaccacgtcc
    53761 gttctcgtag attttacgaa tctcggcgcg cgaagtgata atttccgctt cagtcggata
    53821 atccggcccc tgcacgatat ccagcagctg atcgagcgta gttttcggct ggtcgattaa
    53881 tgcgattgcc gcctgagcta cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc
    53941 gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag gtagcatttt
    54001 cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct gccccagctc
    54061 gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa
    54121 cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg gataacggta
    54181 agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgctatcgc cgtgcggatg
    54241 gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg cgctggcatt
    54301 caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttca gaccatcacc
    54361 aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt aggcgttttc
    54421 cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg attcctcaac
    54481 ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc tgccgcgtcg
    54541 agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg ataaaggcat
    54601 caatgtgcct tgatatacaa ctctttgctg tagacttgat cggcatagag ggcagcacaa
    54661 ggttgcgccc atgccacacc actcggcatg ggggtccgct ccgggcgatt cgagaagttg
    54721 aggttatgcg agtccaggga agcctaaagc atcaaagtag tcgactgtaa gtatacggtt
    54781 cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct cctgccataa gtgcggcttg
    54841 ctcatggata tccccgagac ggcttacggc gcaccggata ataaatttcg cgtatacatg
    54901 gtattcctta ccggagaaat tataagccca acaacaatac gcctgtgaca ttgttcgctc
    54961 aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttccccatca cgccaacggc
    55021 acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt ggtgtagtga
    55081 aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg gcgaataatg
    55141 ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac ggcataacgt
    55201 ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcgg ttcgctaacg
    55261 ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcaggggc gtcccaggcg
    55321 acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata
    55381 aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca
    55441 gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat tttgcctccc
    55501 tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat ttactttgcg
    55561 gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc
    55621 aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt ttgtgatcga tatcaacatt
    55681 gatagtaccg ctggcatctt taaacacgta gagatcgtca gagatgcgtt caacgatatt
    55741 gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta cagtcgttac
    55801 gctgccgttc ggcccctgaa atcctccggc ctgactttgc gttaccgatg ggccagaaaa
    55861 accgccctgc tctgctgcca tcaccggtgc actgcacagg gccattactg cgattactgc
    55921 tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat
    55981 agtccttaac aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac
    56041 aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact
    56101 gatagaagat gacatgctga ttggtgacgg catcaaaact ggccttagta aaatgggttt
    56161 tagtgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata gcgcacctta
    56221 tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata ttttgcgcga
    56281 atggcgagag aaaggtcagc gtgagccggt actgaacctg accgcgcgtg atgcgttagc
    56341 ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt
    56401 gatagaagtc gccgccaggc tggaggctct gatgcgccga accaacggcc aggccagcaa
    56461 cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg
    56521 cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga tgcgtaacgc
    56581 tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt
    56641 caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt
    56701 tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt
    56761 cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt gacctggctg
    56821 ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt gttcgacacc
    56881 caactgatgc tgtttgccaa acggttaagt acgctcgatc tcaacgaaat caacgcggcg
    56941 gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga cgatgcgctg
    57001 acctttgcca tctttaccca cgacggcaga atggtcctta atgatggcga taacggagaa
    57061 gatattccct atagctatca acgggaaggt tttgctgacg ggcaactggt cggtgaagac
    57121 gatccttggc gttttgtctg gatgacctca cctgatggca aatatcgcat cgttgttggc
    57181 caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca attgatcccg
    57241 tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc
    57301 gcgccgctga acaaactggc gctggcacta cgtatgcgtg accctgactc ggaaaaacca
    57361 ctaaacgcga ctggcgtacc cagcgaagtg cggccactgg ttgagtcgct aaatcaactg
    57421 ttcgcccgca cacatgcgat gatggttcga gaacgacgct ttacctccga tgcagctcac
    57481 gaactacgta gcccgttaac agcgctgaaa gtgcaaaccg aagttgcgca gctctctgac
    57541 gatgatccgc aggcgcggaa aaaagcactg ctccaattac attccgggat cgatcgcgct
    57601 actcgtctgg ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag
    57661 gacgtcgcgg agatcccgct tgaagatctc ctacaatcgt cggtgatgga tatttaccac
    57721 acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgcccacgg catcaaacgc
    57781 accggacaac cgctattgct aagtttgttg gtgcgaaatt tgctggataa cggccccggt
    57841 gtgacaccag aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc
    57901 gctaccggta gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatgacatg
    57961 aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt
    58021 ctctgtctat gagagccgtt aaaacgactc tcatagattt tactaatagc aaaatataaa
    58081 ccgtccccaa aaaagccacc aaccacaaac cagacaaata acgagcgaga atacatatag
    58141 ccaaaactcg aacatttccc gcataaagag ttttcttaag ataagaataa taagtggcgt
    58201 aagaagaaaa aatgctgcaa tcattgcttg caatatacga tcttcgcctg aaacggaagg
    58261 tatactgcaa atattgcctc ccgactcaag catccagtca tacttattga aagcaaaaaa
    58321 agaatctatt aaccaataag taagcattat atatggcaat gcttcaaata ttgttttaat
    58381 tttctgcttt aacatatatc agtacgctca taaaaatcca tttataatta tcaacatgac
    58441 acgttgatat tttcatcttt tacttattca aagccatatt ttcaatcccc aaaaaaaccg
    58501 aaccaaacgt gtaaattatt ccggtgcgag taagaattat gaggaatggc tatcagtatt
    58561 gtcattttca gaaaatattt atcctgcatc ggtgagtcag agctagatca gacttttgct
    58621 aaattcgcaa aagactttgc acattttgct aatttcacag taccgctctg tgacgtacta
    58681 tagtcgacaa acgtctcacc ttgagattaa aaaatgagca acatcctgat tatcaacggc
    58741 gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga agtcgcggat
    58801 ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag cgactacaat
    58861 gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca gatgccaggc
    58921 tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt caccgaaggt
    58981 cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccgtcgaa aaaatacggt
    59041 tccggcagcc tggtacagga caaaaaatat atgctttctc taacctggaa cgcaccaatg
    59101 gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gcgttgacgg tgtgtatctg
    59161 ccgttccata aagcaaacca attcctcggt atggaaccgc tgccgacatt tatcgctaat
    59221 gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa gcatcttgtg
    59281 gaaatttttg gttaactaga gctcaggctt tagaaggagt taaccatgct taccgtaatc
    59341 gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cggtattgga tcagtttgct
    59401 aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc aatggtggat
    59461 tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat gattgagcag
    59521 tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc cgcacatgaa ggcgtacagc
    59581 gaagccgtaa aaggcgacgt gctggagatg aatatccgta ttctgcagcc agggatttaa
    59641 tcctgccttg tttgcccggc catcctgacc gggcaatgtt ctttccttta aacctcaatc
    59701 tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc
    59761 agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc
    59821 accagacggc gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg gttcatttcc
    59881 cccagacctt taaaacgctg gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt
    59941 acgccctctt tctcttcttc cgtcagcgcg taataaacct ctttcccgag atcaatacgg
    60001 tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg gaagtgtttt
    60061 acgaacaaag cgcagagcag cgtggcaatg tgcagaccat cagagtccgc atccgcgagg
    60121 atacagattt tgccataacg aagctggctc agatcgtcgc tgtcaggatc gataccgatc
    60181 gctaccgaaa tatcatgcac ttcctgcgag gccagcactt cgtcggaaga aacttcccag
    60241 gtgttaagga tcttaccttt cagcggcatg atcgcctgat attcacgatc gcgcgcctgc
    60301 ttggcagatc cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg gttaaggccc
    60361 tgcgcggtac aatcagccag tttgccaggc agcgccgggc cgctggtcag ctttttacgc
    60421 accacttttt tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccagc
    60481 aactccgccg cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc
    60541 gaaacgaatg ccgcacactg acgcgaagag agacgctctt tggtctgccc ggcaaactgc
    60601 ggatcctgca ttttaaccga cagaacatag gcgcagcgat cccagatatc ttccgccgac
    60661 agcttcacgc cgcgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccaac
    60721 aggccctgac gcagaccatt aacatgggta ccgccctgca tcgttgggat aaggttgacg
    60781 tagctttcgg tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccacagct
    60841 tcagtatcgc cagcgaaatt accgataaac ggtttttctg gcagcgtcgg taaaccgttt
    60901 accgcttccg ccaggtaatc attcagaccg tcctgatagc accagcgctg ttcggtgttg
    60961 ttgatctcat ctttaaaggt aatctcaacg ccggggcaca gtactgcttt ggccttcagc
    61021 acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag
    61081 aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc cgacaacctg taaatcctgc
    61141 accttttcgc cattttcaaa ggcgatgtta taaacctgac catcgcggcg cacgttaact
    61201 tctacgcgct tcgacaaggc gttaactacc gaaatcccca cgccatgcag gccgccagag
    61261 aactggtaat ttttgttaga gaatttaccg cctgcatgca gacggcaaag aatcagttca
    61321 accgccggta ctccttcttc cgggtgaata tccaccggca tcccgcgccc atcgtcaatc
    61381 acttctaacg actggtcagc atgtaagatt acatccacgc gttttgcgtg acccgccagt
    61441 gcttcatcga cactgttatc aataacttct tgccccaaat ggttagggcg agtggtatcg
    61501 gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca
    61561 gcgttataag tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc agagatggtg
    61621 cagaccaaga aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt
    61681 gccgccttct atgacagtct ggcggcagga agcatagtac gccaccgcct ggcggtaatc
    61741 cagcacttca tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc
    61801 aatctgcatg actttgagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt
    61861 gtaggggttc tcgttctgac cgagatagtc cgtcagcaat tcaaacgggc gtaccgccgg
    61921 gtttaccacc actgcgggca gcataaaaca ttgcgacaac caggtggcgt aatatccccc
    61981 cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag
    62041 cagttctgcc gcgtcggaag gatacggcgg caactgcgga atgatcatct caacgtcagg
    62101 gtgatgttcc gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt
    62161 gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg
    62221 aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactcg
    62281 agagtacgcc agccgggcgc gatagtatcc agcgtaaagt tggaacagtg cggcttaaac
    62341 tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc
    62401 tgatgaatat gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc
    62461 agttcgcccg cgttacgcag actgtgttga tcgagccaac tacaacccgc aggtagcgga
    62521 tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc
    62581 cactcaagct gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc
    62641 aacaggattt gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc
    62701 tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca gacgcagggc
    62761 gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc
    62821 gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc
    62881 agcaccgcct ggtagctttc ccaggtgttt acccctaaca gggcttcgtg cttttgtgca
    62941 aacaggtgag tatcggtaat ttgtaaaatc cttcctctgg cctcaccagc cagaggaagg
    63001 gttaacaggc tttccaaatg gtgtccttag gtttcacgac gctagtaaac cggaatcgcc
    63061 atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt aatttgatgc
    63121 ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa
    63181 atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt
    63241 gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg
    63301 tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca
    63361 gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg
    63421 cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt
    63481 tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg
    63541 attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata
    63601 tcttcgtttt catcagccag accatgaata ccgcttgcgg tcgtggcgtc cacttcgccc
    63661 accataattg acgaacgctc actggtgccc cccgggcttg ccaggaaact taacaccggt
    63721 ttggtccgtt tgactatcag tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc
    63781 acactttcac cctcttcaat catcccggca accatctcca gtagccaggg ggtttcgctg
    63841 gtgtcgtacg cggcaatccg aatctgctca atcagcacaa cttcatcacg cactgggtca
    63901 aagggtagca agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc
    63961 atttgcccgt tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga
    64021 taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg
    64081 tctggcttaa gcattgttaa tgtcctagca ctaatagtga attaaatgtg aatttcagcg
    64141 acgtttgact gccgtttgag cagtcatatg ttaaattgag gcacattaac gccctatggc
    64201 acgtaacgcc aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt
    64261 tgatcgcgct aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc
    64321 ttatcggcct gagcctttct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag
    64381 tttatcagca agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg
    64441 ctgcctttga aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg
    64501 cagattacac ctatagcaac ggctaccgcg acgcgaacgg catcaactcg aacgcgacca
    64561 gtgcgtccct gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc
    64621 tgcaggaaaa agcagcaggg attcaggacg tcacatatca gaccgatcag caaaccttga
    64681 tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt ctttcctata
    64741 cacaggcaca aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg
    64801 tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg
    64861 cgaacgaagt gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca
    64921 ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac
    64981 cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg
    65041 cacgcttgag ccaggacctg gagcgcgagc aaattcgcca ggcgcaggat ggtcacttac
    65101 cgactctgga tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa
    65161 cccgtggtgc cgctggtacc cagtatgacg atagcaatat gggccagaac aaagttggcc
    65221 tgagcttctc gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggcac
    65281 agtacaactt tgttggtgcc agtgagcaac tggaaagcgc acatcgtagc gtcgtgcaaa
    65341 ccgtacgttc ctccttcaac aacattaatg cttctatcag tagtattaac gcctacaaac
    65401 aagccgtagt ttccgcgcaa agctcattag acgcgatgga agcgggctac tcggtcggta
    65461 cgcgtaccat tgttgatgtg ttggatgcga ccaccacgct gtacaacgcc aagcaagagc
    65521 tggcgaatgc gcgttataac tacctgatta atcagctgaa tattaagtca gccctgggta
    65581 cgttgaacga gcaggatttg ctggcactga acaatgcgct gagcaaaccg gtttccacta
    65641 atccggaaaa cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc
    65701 ctgatagccc ggcacccgtc gttcagcaaa catccgcacg cactaccacc agtaacggtc
    65761 ataacccttt ccgtaactga tgacgacgac ggggcttcgg ccccgtctga acgtaaggca
    65821 acgtaaagat acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc
    65881 catcctctat tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa
    65941 aatccatacg ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg
    66001 ctctcgcggt tgccactgtt tttatgctgg ctggctgtga aaagagtgat gaaacagtgt
    66061 ctctctatca aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta
    66121 ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc
    66181 gtgaagactg tgttgctgaa tttggtgaag gtcagtgtca gcaagcacca gcccaggctg
    66241 gcatggcacc agaaaaccag gcgcaggctc agcaatccag cgggagtttc tggatgccgc
    66301 tgatggccgg ttacatgatg gggcgtctga tgggcggcgg cgcgggattt gcacagcagc
    66361 cgctgttctc ctcgaaaaac ccggccagtc cggcttacgg taaatatacc gacgcgacgg
    66421 gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg
    66481 caccaaaacc ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa tctgttgcca
    66541 aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat
    66601 accgatggaa agagtcagta ttaccgagcg cccggactgg cgtgagaaag cccacgaata
    66661 cggtttcaat tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa
    66721 gttgaccctc gcccaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg
    66781 cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg atgaccaaat tccgcatacc
    66841 aaaacacacc tggagttttg tgcgtcaatc atggctgact caccagccat cgctttattc
    66901 gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga
    66961 tacgccaacg tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct
    67021 taacgccggt aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaaactgat
    67081 cgatcgcttc gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg
    67141 tcgcgacacg gtggaagatc gcggaaccat tcagtatttg caggactgcg caaccgaagc
    67201 tgaaatcgcc accgagttcc tctacatcga cgagataggt ttaggcgaaa aaggccagtt
    67261 cacggattta caggatcagg tgatttctaa cctgttcaag ctttatccgt gggaatttat
    67321 gctgcgcgaa atgttctcca ccaagctgga agatactggc gtacgctggc tggaaccggc
    67381 gtggaagagc attatctcca acaaggcact tctaccgcta ctgtgggaga tgttcccgaa
    67441 tcacccgaac ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata
    67501 tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa
    67561 aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca
    67621 cccgttaccg aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca
    67681 acctgccgga attggcattc gtgaagaccg tgcgttgatc acccaggata tgtctcggtt
    67741 ttatccacat atttttgttg aataagccac gataccggat ggcactcgcc atccggtaat
    67801 tgttagccta tctgcaccga cagcatactt aaactgccca tttctatacc atcgaccgga
    67861 atggtaattg gctcctgccc atcccacaca cctaacacat acaacaacgg cagatagtgc
    67921 tctggcgttg ggttcgataa cgcgccacct tcatggtcga gataattcac cagaggatgc
    67981 tgctctaccg gcccttgcca cgtcagattc gctttcacat actcattaaa cgacgtcgcc
    68041 cacggatacg gtgaactatc accgtgccac ttcactgtgc gcaggttatg caccacgtta
    68101 ccgctggcga ccaacattat tccttcatca cgcagcgccg cgagtttgcg ccccatttcg
    68161 aaatgccagg cggcaggttt gctactgtcg atactcaact gcaccatagg gatatcggcg
    68221 tcaggataca tcttaatcag cacgccccac gagccgtggt caaagcccca ggcttcttta
    68281 tccagcgtca ccgggatcgg cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa
    68341 cccggagcag gataatgcgt atcgtacagc gcctgcggga agccaccaaa gtcatgaatc
    68401 gtgggcggcg tctccatcgc ggtcactcct gttccacggg taaaccagtg agccgaaacc
    68461 accacaatcg cttgcgggcg tggcaatgtc atccccaact tctgccagct gcgggtatac
    68521 aaattatctt ccagcacgtt catcggacta ccgtgaccta aaaacaatgc tggcatacgt
    68581 gttgaagaca tgatgatatc cttaactaaa ggtgtcattt tgatatcctc acaatacgct
    68641 tgttcggcgg agtaagaacc cggataacaa tgatgatgat catcagttat tttgacgatc
    68701 tgcctgaagg tgaagattta taaggagttg tcgatgtcag tacctctcat tctgaccata
    68761 ctggcggggg cagccacgtt tattggcgcg tttctcggcg ttctcggaca aaaaccctcg
    68821 aaccgcttac tggcgttttc gctaggtttt gcggcgggga tcatgttgct catctcatta
    68881 atggaaatgc ttcctgccgc actagcagct gaaggaatgt cgccggtgtt gggttatgga
    68941 atgtttatct tcggtctgct tggctacttt ggcctggacc gcatgttgcc gcatgcgcat
    69001 ccgcaggatt taatgcaaaa atcggtgcag ctgttgccaa aatcgatcaa gcgcacagcc
    69061 attctgctca ctctcggcat cagtctgcat aacttcccgg aagggattgc cacctttgtc
    69121 acggcgagca gcaacctgga gctgggattt ggcatcgcac tggccgtcgc gttgcacaat
    69181 atccctgaag gtctggcagt ggcaggcccg gtttatgcgg caacggggtc taaacgcacc
    69241 gcgattctgt gggcggggat ttctggacta gcagaaattc ttggtggtgt gctggcgtgg
    69301 ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg caatcatggc ggcggttgca
    69361 ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct
    69421 aataataacc ccagctatgg cgtactatgt ggaatgtcag tgatgggatt cagtttagtg
    69481 ctgctacaaa cggcgggaat tggttaaaaa caacatcaga agttgtccag cacatggaca
    69541 acttctgcca gcttcacgaa agccttattc taccttttcg gctgcctctt ctctcgtact
    69601 gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca
    69661 ataacgtttc aaatgcagga aataacccac cacaaccaga gcaatcaata caatccagta
    69721 ccaggcgata aacatccttt tacccttaat atttaacaat actaaacatt tagcgcataa
    69781 atttcacata tcctttttcg gatatattca tatggtcgca taataacgga acaattatat
    69841 gattttgatc ataatgaaaa agaatgcctg atattaatga aattttctca tagaaagaga
    69901 agcggaatta acatcttgca tagcagggca aaataaaaac cgaagccagc tacaacttcg
    69961 gtttcttgat taaggcagta attaagcagc ggttttcagc tggctttacg ctcatgtgcc
    70021 tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt attggcaaac
    70081 tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc acacagcaca
    70141 ccagccggtt taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca
    70201 cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga
    70261 tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg atcagcggca
    70321 gaaacaccgg tagtcacacc ttcagccgct tcaatggtca cggtaaaacc ggtgccatag
    70381 gcgctggtgt tattttctac catcattggc agatcaagtt gtttacggcg atcttcagtg
    70441 atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc
    70501 atggtttctg ccgggaagat catatcgcct tcgttttcac ggtcttcatc atcaagcacc
    70561 attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc gaaaggcgta
    70621 ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa ttatggttac
    70681 cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga gcgggtccat
    70741 gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc ggaattacac
    70801 cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat
    70861 ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact ataacgctaa
    70921 tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta cccttatcgc
    70981 actacaatgg tactcaacac ctatcagtac agggaaacta caatgattga cccgaaaaaa
    71041 attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag ggagttcggg
    71101 gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg cctcgacctt
    71161 gtaagccgtg aagagttcga cgtccaaacg caagtcctgt tacggactcg tgagaaactg
    71221 gcgctgcttg aacaaagaat cagcgaactg gaaaatcgca gtactgagat caaaaagcag
    71281 ccagatccag agactctccc cccaacgctg taaacatttg tctttaatgc actgtaaatt
    71341 atgaattaat cctctaattt acagtgtttt ctattacatt atcgcttaat cccattcaga
    71401 gtatcatcac aaaaatgaat gattgcaatt gaaaatataa ttgttatgta ttctttaagt
    71461 tttacttaat cgata
//
LOCUS       DAJVQR010000006        70703 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVQR010000006 DAJVQR010000000
VERSION     DAJVQR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..795
                     /locus_tag="OQM52_000418"
     CDS             <1..795
                     /locus_tag="OQM52_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1562397.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="OQM52_000419"
     CDS             806..1897
                     /locus_tag="OQM52_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1562398.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OQM52_000420"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OQM52_000420"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1562399.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="OQM52_000421"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="OQM52_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1562400.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="OQM52_000422"
     CDS             4859..5254
                     /locus_tag="OQM52_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1562401.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="OQM52_000423"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="OQM52_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1562402.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="OQM52_000424"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="OQM52_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1562403.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="OQM52_000425"
     CDS             complement(6563..7603)
                     /locus_tag="OQM52_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1562404.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="OQM52_000426"
     CDS             complement(7676..8311)
                     /locus_tag="OQM52_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1562405.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="OQM52_000427"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="OQM52_000427"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1562406.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="OQM52_000428"
     CDS             complement(8937..9380)
                     /locus_tag="OQM52_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1562407.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="OQM52_000429"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="OQM52_000429"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1562408.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="OQM52_000430"
     CDS             complement(9720..10223)
                     /locus_tag="OQM52_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1562409.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="OQM52_000431"
     CDS             complement(10217..10741)
                     /locus_tag="OQM52_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1562410.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="OQM52_000432"
     CDS             10950..11945
                     /locus_tag="OQM52_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1562411.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="OQM52_000433"
     CDS             11954..12832
                     /locus_tag="OQM52_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1562412.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGTVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="OQM52_000434"
     CDS             12913..13920
                     /locus_tag="OQM52_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1562413.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OQM52_000435"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OQM52_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1562414.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OQM52_000436"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OQM52_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1562415.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OQM52_000437"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OQM52_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1562416.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OQM52_000438"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OQM52_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1562417.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OQM52_000439"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OQM52_000439"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1562418.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OQM52_000440"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OQM52_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1562419.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OQM52_000441"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OQM52_000441"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1562420.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OQM52_000442"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OQM52_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1562421.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OQM52_000443"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OQM52_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1562422.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OQM52_000444"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OQM52_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1562423.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OQM52_000445"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OQM52_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1562424.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="OQM52_000446"
     tRNA            complement(27654..27730)
                     /locus_tag="OQM52_000446"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="OQM52_000447"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="OQM52_000447"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1562425.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="OQM52_000448"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="OQM52_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="OQM52_000449"
     tRNA            complement(31514..31600)
                     /locus_tag="OQM52_000449"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OQM52_000450"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OQM52_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1562426.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OQM52_000451"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OQM52_000451"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1562427.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OQM52_000452"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OQM52_000452"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1562428.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OQM52_000453"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OQM52_000453"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1562429.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OQM52_000454"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OQM52_000454"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1562430.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="OQM52_000455"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="OQM52_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1562431.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OQM52_000456"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OQM52_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1562432.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="OQM52_000457"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="OQM52_000457"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1562433.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OQM52_000458"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OQM52_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1562434.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="OQM52_000459"
     CDS             complement(41212..42177)
                     /locus_tag="OQM52_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1562435.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OQM52_000460"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OQM52_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1562436.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OQM52_000461"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OQM52_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1562437.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="OQM52_000462"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="OQM52_000462"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1562438.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="OQM52_000463"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="OQM52_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1562439.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OQM52_000464"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OQM52_000464"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1562440.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OQM52_000465"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OQM52_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1562441.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OQM52_000466"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OQM52_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1562442.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OQM52_000467"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OQM52_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1562443.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OQM52_000468"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OQM52_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1562444.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OQM52_000469"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OQM52_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1562445.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OQM52_000470"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OQM52_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1562446.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRRIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="OQM52_000471"
     CDS             49715..50680
                     /locus_tag="OQM52_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1562447.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="OQM52_000472"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="OQM52_000472"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1562448.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="OQM52_000473"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="OQM52_000473"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1562449.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="OQM52_000474"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="OQM52_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1562450.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="OQM52_000475"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="OQM52_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1562451.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="OQM52_000476"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="OQM52_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1562452.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="OQM52_000477"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="OQM52_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1562453.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="OQM52_000478"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="OQM52_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1562454.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="OQM52_000479"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="OQM52_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1562455.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="OQM52_000480"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="OQM52_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1562456.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="OQM52_000481"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="OQM52_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1562457.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="OQM52_000482"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="OQM52_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1562458.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="OQM52_000483"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="OQM52_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="OQM52_000484"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="OQM52_000484"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1562459.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="OQM52_000485"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="OQM52_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1562460.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="OQM52_000486"
     CDS             complement(62547..63476)
                     /locus_tag="OQM52_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1562461.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="OQM52_000487"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="OQM52_000487"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1562462.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="OQM52_000488"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="OQM52_000488"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1562463.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="OQM52_000489"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="OQM52_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
BASE COUNT        17309 a        19175 c        17557 g        16662 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cactgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cggcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aat
//
LOCUS       DAJVQR010000007        67661 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVQR010000007 DAJVQR010000000
VERSION     DAJVQR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..228
                     /locus_tag="OQM52_000490"
     CDS             <1..228
                     /locus_tag="OQM52_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCS1562464.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OQM52_000491"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OQM52_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OQM52_000492"
     CDS             1365..1688
                     /locus_tag="OQM52_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCS1562465.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OQM52_000493"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OQM52_000493"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1562466.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OQM52_000494"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OQM52_000494"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1562467.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OQM52_000495"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OQM52_000495"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1562468.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OQM52_000496"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OQM52_000496"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1562469.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OQM52_000497"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OQM52_000497"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1562470.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OQM52_000498"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OQM52_000498"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1562471.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OQM52_000499"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OQM52_000499"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1562472.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OQM52_000500"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OQM52_000500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1562473.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OQM52_000501"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OQM52_000501"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1562474.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OQM52_000502"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OQM52_000502"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1562475.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OQM52_000503"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OQM52_000503"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1562476.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OQM52_000504"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OQM52_000504"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1562477.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OQM52_000505"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OQM52_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1562478.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OQM52_000506"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OQM52_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1562479.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OQM52_000507"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OQM52_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1562480.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OQM52_000508"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OQM52_000508"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1562481.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OQM52_000509"
     CDS             complement(21226..23058)
                     /locus_tag="OQM52_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1562482.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OQM52_000510"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OQM52_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1562483.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OQM52_000511"
     CDS             complement(23806..24300)
                     /locus_tag="OQM52_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1562484.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OQM52_000512"
     CDS             complement(24383..24838)
                     /locus_tag="OQM52_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1562485.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OQM52_000513"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OQM52_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1562486.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OQM52_000514"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OQM52_000514"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1562487.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OQM52_000515"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OQM52_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1562488.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OQM52_000516"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OQM52_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1562489.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OQM52_000517"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OQM52_000517"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1562490.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OQM52_000518"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OQM52_000518"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1562491.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OQM52_000519"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OQM52_000519"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1562492.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OQM52_000520"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OQM52_000520"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1562493.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OQM52_000521"
     CDS             33414..34307
                     /locus_tag="OQM52_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1562494.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OQM52_000522"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OQM52_000522"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1562495.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OQM52_000523"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OQM52_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1562496.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OQM52_000524"
     CDS             complement(36223..36939)
                     /locus_tag="OQM52_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1562497.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OQM52_000525"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OQM52_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1562498.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OQM52_000526"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OQM52_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1562499.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OQM52_000527"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OQM52_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1562500.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OQM52_000528"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OQM52_000528"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1562501.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OQM52_000529"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OQM52_000529"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1562502.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OQM52_000530"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OQM52_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1562503.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OQM52_000531"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OQM52_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1562504.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OQM52_000532"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OQM52_000532"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1562505.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OQM52_000533"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OQM52_000533"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1562506.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OQM52_000534"
     CDS             complement(45788..46447)
                     /locus_tag="OQM52_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1562507.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OQM52_000535"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OQM52_000535"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1562508.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OQM52_000536"
     CDS             complement(47342..48355)
                     /locus_tag="OQM52_000536"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1562509.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OQM52_000537"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OQM52_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1562510.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVYHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OQM52_000538"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OQM52_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1562511.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OQM52_000539"
     CDS             complement(50648..51826)
                     /locus_tag="OQM52_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1562512.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OQM52_000540"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OQM52_000540"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1562513.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OQM52_000541"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OQM52_000541"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1562514.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OQM52_000542"
     CDS             complement(55608..55886)
                     /locus_tag="OQM52_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1562515.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OQM52_000543"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OQM52_000543"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1562516.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OQM52_000544"
     CDS             complement(56468..57277)
                     /locus_tag="OQM52_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1562517.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OQM52_000545"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OQM52_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1562518.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OQM52_000546"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OQM52_000546"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1562519.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OQM52_000547"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OQM52_000547"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1562520.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OQM52_000548"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OQM52_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1562521.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OQM52_000549"
     CDS             complement(60978..61868)
                     /locus_tag="OQM52_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1562522.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OQM52_000550"
     CDS             complement(61855..62379)
                     /locus_tag="OQM52_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1562523.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OQM52_000551"
     CDS             complement(62376..62846)
                     /locus_tag="OQM52_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1562524.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OQM52_000552"
     CDS             complement(62843..63391)
                     /locus_tag="OQM52_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1562525.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OQM52_000553"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OQM52_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OQM52_000554"
     CDS             complement(64138..64860)
                     /locus_tag="OQM52_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1562526.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OQM52_000555"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OQM52_000555"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OQM52_000556"
     CDS             complement(66023..66778)
                     /locus_tag="OQM52_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1562527.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..67423)
                     /locus_tag="OQM52_000557"
     CDS             complement(66860..67423)
                     /locus_tag="OQM52_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1562528.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16921 a        18281 c        16781 g        15678 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgtttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catacccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaagaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagg ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgataaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa ttgtattcat
    67561 tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt ttactaaaga
    67621 gtaaatgtag atttaattaa tatattgaca gaagaaaggt t
//
LOCUS       DAJVQR010000008        66977 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVQR010000008 DAJVQR010000000
VERSION     DAJVQR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(57..1061)
                     /gene="cra"
                     /locus_tag="OQM52_000558"
     CDS             complement(57..1061)
                     /gene="cra"
                     /locus_tag="OQM52_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1562529.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1143..1229)
                     /locus_tag="OQM52_000559"
     CDS             complement(1143..1229)
                     /locus_tag="OQM52_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1562530.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1241..1732)
                     /gene="ilvN"
                     /locus_tag="OQM52_000560"
     CDS             complement(1241..1732)
                     /gene="ilvN"
                     /locus_tag="OQM52_000560"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1562531.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1735..3459)
                     /gene="ilvI"
                     /locus_tag="OQM52_000561"
     CDS             complement(1735..3459)
                     /gene="ilvI"
                     /locus_tag="OQM52_000561"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1562532.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3578..3622
                     /locus_tag="OQM52_000562"
     CDS             3578..3622
                     /locus_tag="OQM52_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562533.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3777..4721)
                     /gene="leuO"
                     /locus_tag="OQM52_000563"
     CDS             complement(3777..4721)
                     /gene="leuO"
                     /locus_tag="OQM52_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1562534.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5380..5466
                     /gene="leuL"
                     /locus_tag="OQM52_000564"
     CDS             5380..5466
                     /gene="leuL"
                     /locus_tag="OQM52_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1562535.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5559..7130
                     /gene="leuA"
                     /locus_tag="OQM52_000565"
     CDS             5559..7130
                     /gene="leuA"
                     /locus_tag="OQM52_000565"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1562536.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7130..8221
                     /gene="leuB"
                     /locus_tag="OQM52_000566"
     CDS             7130..8221
                     /gene="leuB"
                     /locus_tag="OQM52_000566"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1562537.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8224..9624
                     /gene="leuC"
                     /locus_tag="OQM52_000567"
     CDS             8224..9624
                     /gene="leuC"
                     /locus_tag="OQM52_000567"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1562538.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9635..10240
                     /gene="leuD"
                     /locus_tag="OQM52_000568"
     CDS             9635..10240
                     /gene="leuD"
                     /locus_tag="OQM52_000568"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1562539.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10289..11467)
                     /gene="setA"
                     /locus_tag="OQM52_000569"
     CDS             complement(10289..11467)
                     /gene="setA"
                     /locus_tag="OQM52_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS1562540.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11569..11700)
                     /gene="sgrT"
                     /locus_tag="OQM52_000570"
     CDS             complement(11569..11700)
                     /gene="sgrT"
                     /locus_tag="OQM52_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1562541.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11789..13444
                     /gene="sgrR"
                     /locus_tag="OQM52_000571"
     CDS             11789..13444
                     /gene="sgrR"
                     /locus_tag="OQM52_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1562542.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13478..13577
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13608..14591
                     /gene="thiB"
                     /locus_tag="OQM52_000572"
     CDS             13608..14591
                     /gene="thiB"
                     /locus_tag="OQM52_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1562543.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14567..16177
                     /gene="thiP"
                     /locus_tag="OQM52_000573"
     CDS             14567..16177
                     /gene="thiP"
                     /locus_tag="OQM52_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1562544.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16161..16859
                     /gene="thiQ"
                     /locus_tag="OQM52_000574"
     CDS             16161..16859
                     /gene="thiQ"
                     /locus_tag="OQM52_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1562545.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16973..17737)
                     /locus_tag="OQM52_000575"
     CDS             complement(16973..17737)
                     /locus_tag="OQM52_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1562546.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17823..18701)
                     /gene="araC"
                     /locus_tag="OQM52_000576"
     CDS             complement(17823..18701)
                     /gene="araC"
                     /locus_tag="OQM52_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1562547.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19040..20740
                     /gene="araB"
                     /locus_tag="OQM52_000577"
     CDS             19040..20740
                     /gene="araB"
                     /locus_tag="OQM52_000577"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1562548.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20751..22253
                     /gene="araA"
                     /locus_tag="OQM52_000578"
     CDS             20751..22253
                     /gene="araA"
                     /locus_tag="OQM52_000578"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1562549.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22453..23148
                     /gene="araD"
                     /locus_tag="OQM52_000579"
     CDS             22453..23148
                     /gene="araD"
                     /locus_tag="OQM52_000579"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1562550.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23223..25574
                     /gene="polB"
                     /locus_tag="OQM52_000580"
     CDS             23223..25574
                     /gene="polB"
                     /locus_tag="OQM52_000580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1562551.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25739..28645
                     /gene="rapA"
                     /locus_tag="OQM52_000581"
     CDS             25739..28645
                     /gene="rapA"
                     /locus_tag="OQM52_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1562552.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28657..29316
                     /gene="rluA"
                     /locus_tag="OQM52_000582"
     CDS             28657..29316
                     /gene="rluA"
                     /locus_tag="OQM52_000582"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1562553.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29433..30248)
                     /gene="djlA"
                     /locus_tag="OQM52_000583"
     CDS             complement(29433..30248)
                     /gene="djlA"
                     /locus_tag="OQM52_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1562554.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30503..32857
                     /gene="lptD"
                     /locus_tag="OQM52_000584"
     CDS             30503..32857
                     /gene="lptD"
                     /locus_tag="OQM52_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1562555.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32910..34196
                     /gene="surA"
                     /locus_tag="OQM52_000585"
     CDS             32910..34196
                     /gene="surA"
                     /locus_tag="OQM52_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1562556.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34196..35185
                     /gene="pdxA"
                     /locus_tag="OQM52_000586"
     CDS             34196..35185
                     /gene="pdxA"
                     /locus_tag="OQM52_000586"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1562557.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35182..36003
                     /gene="rsmA"
                     /locus_tag="OQM52_000587"
     CDS             35182..36003
                     /gene="rsmA"
                     /locus_tag="OQM52_000587"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1562558.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36006..36383
                     /gene="apaG"
                     /locus_tag="OQM52_000588"
     CDS             36006..36383
                     /gene="apaG"
                     /locus_tag="OQM52_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1562559.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36390..37232
                     /gene="apaH"
                     /locus_tag="OQM52_000589"
     CDS             36390..37232
                     /gene="apaH"
                     /locus_tag="OQM52_000589"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1562560.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37310..37789)
                     /gene="folA"
                     /locus_tag="OQM52_000590"
     CDS             complement(37310..37789)
                     /gene="folA"
                     /locus_tag="OQM52_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1562561.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37980..39842)
                     /gene="kefC"
                     /locus_tag="OQM52_000591"
     CDS             complement(37980..39842)
                     /gene="kefC"
                     /locus_tag="OQM52_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1562562.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39835..40365)
                     /gene="kefF"
                     /locus_tag="OQM52_000592"
     CDS             complement(39835..40365)
                     /gene="kefF"
                     /locus_tag="OQM52_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1562563.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40413..41807)
                     /locus_tag="OQM52_000593"
     CDS             complement(40413..41807)
                     /locus_tag="OQM52_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1562564.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41866..42153)
                     /gene="fixX"
                     /locus_tag="OQM52_000594"
     CDS             complement(41866..42153)
                     /gene="fixX"
                     /locus_tag="OQM52_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS1562565.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42150..43436)
                     /locus_tag="OQM52_000595"
     CDS             complement(42150..43436)
                     /locus_tag="OQM52_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1562566.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43487..44428)
                     /locus_tag="OQM52_000596"
                     /pseudo
     CDS             complement(43487..44428)
                     /locus_tag="OQM52_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44443..45213)
                     /locus_tag="OQM52_000597"
     CDS             complement(44443..45213)
                     /locus_tag="OQM52_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS1562567.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45569..47083
                     /gene="caiT"
                     /locus_tag="OQM52_000598"
     CDS             45569..47083
                     /gene="caiT"
                     /locus_tag="OQM52_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS1562568.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47114..48256
                     /gene="caiA"
                     /locus_tag="OQM52_000599"
     CDS             47114..48256
                     /gene="caiA"
                     /locus_tag="OQM52_000599"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS1562569.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48385..49602
                     /gene="caiB"
                     /locus_tag="OQM52_000600"
     CDS             48385..49602
                     /gene="caiB"
                     /locus_tag="OQM52_000600"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCS1562570.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49676..51229
                     /gene="caiC"
                     /locus_tag="OQM52_000601"
     CDS             49676..51229
                     /gene="caiC"
                     /locus_tag="OQM52_000601"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS1562571.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51338..52123
                     /gene="caiD"
                     /locus_tag="OQM52_000602"
     CDS             51338..52123
                     /gene="caiD"
                     /locus_tag="OQM52_000602"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS1562572.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52129..52719
                     /gene="caiE"
                     /locus_tag="OQM52_000603"
     CDS             52129..52719
                     /gene="caiE"
                     /locus_tag="OQM52_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS1562573.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52805..53200)
                     /gene="caiF"
                     /locus_tag="OQM52_000604"
     CDS             complement(52805..53200)
                     /gene="caiF"
                     /locus_tag="OQM52_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS1562574.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53462..56683)
                     /gene="carB"
                     /locus_tag="OQM52_000605"
     CDS             complement(53462..56683)
                     /gene="carB"
                     /locus_tag="OQM52_000605"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1562575.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56701..57849)
                     /gene="carA"
                     /locus_tag="OQM52_000606"
     CDS             complement(56701..57849)
                     /gene="carA"
                     /locus_tag="OQM52_000606"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1562576.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58305..59126)
                     /gene="dapB"
                     /locus_tag="OQM52_000607"
     CDS             complement(58305..59126)
                     /gene="dapB"
                     /locus_tag="OQM52_000607"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1562577.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59293..60207)
                     /gene="rihC"
                     /locus_tag="OQM52_000608"
     CDS             complement(59293..60207)
                     /gene="rihC"
                     /locus_tag="OQM52_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1562578.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60273..61223)
                     /gene="ispH"
                     /locus_tag="OQM52_000609"
                     /gene_synonym="lytB"
     CDS             complement(60273..61223)
                     /gene="ispH"
                     /locus_tag="OQM52_000609"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1562579.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61225..61674)
                     /gene="fkpB"
                     /locus_tag="OQM52_000610"
     CDS             complement(61225..61674)
                     /gene="fkpB"
                     /locus_tag="OQM52_000610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1562580.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61761..62255)
                     /gene="lspA"
                     /locus_tag="OQM52_000611"
     CDS             complement(61761..62255)
                     /gene="lspA"
                     /locus_tag="OQM52_000611"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1562581.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62255..65071)
                     /gene="ileS"
                     /locus_tag="OQM52_000612"
     CDS             complement(62255..65071)
                     /gene="ileS"
                     /locus_tag="OQM52_000612"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1562582.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65114..66055)
                     /gene="ribF"
                     /locus_tag="OQM52_000613"
     CDS             complement(65114..66055)
                     /gene="ribF"
                     /locus_tag="OQM52_000613"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1562583.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66063..66281)
                     /locus_tag="OQM52_000614"
     CDS             complement(66063..66281)
                     /locus_tag="OQM52_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1562584.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66384..66647
                     /gene="rpsT"
                     /locus_tag="OQM52_000615"
     CDS             66384..66647
                     /gene="rpsT"
                     /locus_tag="OQM52_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1562585.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16244 a        18237 c        17243 g        15253 t
ORIGIN      
        1 tcccgtactc tacgcgccag agggaaattc acctggcgcg tatttttgtt cgcggattag
       61 ctacggctga gcacgccgcg gcgatagaga ttacgtttaa tgcgcgttaa accaggtttt
      121 ggcttacgcg gttcgtccag gcttgccagg acaatctcca gcacacgctc tgcgacatcg
      181 cggtgacgtt gagccactgc cagcaccgga cactgtaaga agtcgagcag ttcgttatcg
      241 ccaaaggtgg caattgccag gtcagaaggc agtttgccgt cgcgacgcag cgtgacatcc
      301 atcactcctt gcaacaacgc aaacgacgtt gtgaacagcg cctgcggcat cggatgcgtt
      361 tccagccatt tttcgaataa ctgggcagcc gcctcccgct catagctgtt ggcatacagg
      421 aaatgcactt cgcgcggatc atctttccaa gcagtacgga aaccttgttc acgcaggaag
      481 ctgacagaaa gctccggcag cgcgccaaga taaagcaccg tctcggcggg aaacttacgt
      541 aactcttccg ccagcatttc ggcatcatcc tgatcggcac caaccacgct ggtgaaatgt
      601 tcacgatcga gggcgcggtc cagcgcgaca atcgggaacg ggtcgttagc ccagcgttga
      661 taaaaaggat gctcaggtgg caacgacgtc gaaacaataa tggcatcaac ctgacgctgt
      721 aaaaggtgct caatgcagcg catttcgttg tctggctgat cttctgagca ggcaatcagc
      781 agttgataac cccgttgccg cgcctggcgt tcaagatagt tagcgatgcg ggtatagctg
      841 gtgttctcca gatcggggat cacaagacca atagaacgtg tgcgtccagc acgaagccca
      901 gctgccacgg cgttcgggtg gtaattgtgc tcacgcacca cagccatgac tttttcaacg
      961 gttttgtcgc tcacacggta ttgcttcgct ttgccgttaa taacatagct tgcagtggtc
     1021 cgcgacactc ccgccagccg agcgatttca tccagtttca caattgcccc ttgcgtaaaa
     1081 tgtaaaaacc atagccattg tactggtatg ggttaaattc attaacatct aagcgcagaa
     1141 aattaactgc ggcaactgct tttattccag gttcccactg atttcgcaaa aaaagcccaa
     1201 cgtgacatgt tgggctgcaa attgcgcact gagatcattc tcaacgcatt attttatcgc
     1261 cgcgcgaaag tccgaccaca ccagagcgag caacttccac aattttcgcc acatcgcgaa
     1321 tcgatgctaa aaatgcatca agcttatcgc tggtgcctgc taattgaacg gtataaagcg
     1381 agggggtgac atcgataatt tgcccacgga atatttccgt attacgtttc acttcgtcac
     1441 gcccgtaacc gctggcctga attttcacca gcatgatttc ccgctcaaca tgcgcgccct
     1501 gccccaactc actcacgcgc aagacatcga ccagcttgtg taattgcttt tcgatctgct
     1561 caagtacttt ttcatcgccc acggtctgga tggtcatacg cgataatgtc ggatcgtcgg
     1621 ttggcgcaac ggtcaggctt tcaatgttgt agcctcgctg ggaaaaaagg ccaatcacgc
     1681 gggataacgc gcctgattca ttttcgagta agactgataa tatccggcgc ataatcaggt
     1741 tctctccgtt ttgcttaacc acatttcatc cattccgccc ccgcgaatct gcatcgggta
     1801 gacgtgctca ctgccatcga cggtaacatc aacaaacacc aggcgattat tgcgcacctg
     1861 ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga tgagagatct ggatcccgac
     1921 gtgaccatag gcttccgcca gacggacgaa atcgggtagt gattgcatat aagattgtga
     1981 atgacggccg gaatagatca tgtcctgcca ctgtttcacc atccccagat agcggttatt
     2041 gagattcacc accagtacgg gcaactcgta ttgcaacgcg gtagacagtt cctggatgtt
     2101 catctgaata ctgccgtcgc cagtgacgca aaccacggtt tcttctggca acgccatttt
     2161 gacgcccagt gccgcaggta aaccaaaacc catcgtgccg agaccgccgg aattgatcca
     2221 gcgacgcggt ttgtcgaatg gataataaag cgcagcaaac atctggtgct gcccgacatc
     2281 ggacgtcacg taagcgtccc cattagtcaa ccgccaaaga gtctcgatca ccgcctgcgg
     2341 tttaatcttt tcactgtgag tgtcatattt caggcactga agagcgcgcc actgttcaat
     2401 ctgctgccac cagtcgcgga tctcatccag cggttgatgg gcggattctt gcgacaggag
     2461 ttcaagcatt tgttcgagga cctggcgagc atcccccaca atcgggatat ccgcagtcac
     2521 ggttttagaa atggaagtag gatcaatatc gatatgcaga acagtggcat ttgggcagta
     2581 ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc ccgacggcga aaatcacatc
     2641 cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc attcccagca tgcccagtgc
     2701 ctgacgatgc gttgccggaa acgcccccat tcccatcaat gaggaaacaa cgggcagatt
     2761 caacgcctcc accgtttctt tcaactgctg atggcagccc gccatgattg ccccaccgcc
     2821 tacgtagaca accggttttt ttgccgctac cagcgtttgc agagcacgct taatttgccc
     2881 tttatgtccg gtagtagtgg gattgtaaga acgcatactg accgactcag ggcagacata
     2941 gggtaatttg ttcgccggat taagaatatc tttcggtaaa tcaacgacca ctggtcctgg
     3001 gcgaccactt gccgccagcc agaaagcctt tttcagcacc tgcggaatgt cttccgtttg
     3061 cttaaccaga aaactgtgtt taaccaccgg tcgcgaaatc cccaccatgt cgcactcctg
     3121 aaaggcatcg taacctatca acgaggtcgc tacctgcccg gaaaggacaa ctaatggaat
     3181 ggaatccata taagcggtgg cgatgccggt aatcgcattg gtcgcccttg gacccgacgt
     3241 taccagcacg acgccgactt ccccggtcgc acgcgccagg ccatcggcca tatgcaccgc
     3301 cgcctgctca tgacgaacta atacatgatc aataccaccc acggtatgca atgcatcgta
     3361 aatatcaagg actgcgcctc cgggataacc gaatacttgt ttaacgccct gatcgataag
     3421 cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca ctgtttgacg
     3481 gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg cagccagaga
     3541 cataaacgca gggtaataag aataaacagg aggaaaggtg aaaagaaacg ataaaatcct
     3601 ccattacgaa ggattaaatt gataataaat cggctgaatc ccacaactta cagcaatttc
     3661 tctatttttt taattaatcc taaaatagag agtccacaca ttcagaataa aattctacat
     3721 tcaataaaaa atgcagaata aaccagacat tcatgtctgg cctattctgc attcagttag
     3781 cgtttgcaaa ttgagactaa ttgttcttcc atccactgat ggcctttatc gcgcccggca
     3841 gcttcatgcc aagagagata acaggttctg ctgttttgtt ttaacggcag cggtaatacc
     3901 tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat agcgaccaaa
     3961 tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata cgcgatactg
     4021 gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg acgcgaaacg atcgagcgaa
     4081 accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccttt aattgttgga
     4141 tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct ggtaaattca
     4201 ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg cagctgatgt
     4261 tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc aatctgctca
     4321 atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac acaaagatga
     4381 aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt ttgtactagt
     4441 tgcaatgcct ggcgaactga accaaaaagt tgaaatgcgc gagcagtcgg ttgaatacca
     4501 cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc aacagcgtta
     4561 ctgaccgcag gttgcgacat acccagaaca tgagcggcac gggtaatgtt ttgctcctgc
     4621 atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg tggcttgctt
     4681 aactccgccg tctctgaatg atctgtttgt acctctggca tacttaactc cactgtcaca
     4741 cttaactccc tttcccttat tggaatgcag agaaataatc ctgaagaata tgatttatca
     4801 tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa gatacgggtt
     4861 ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat aataatccat
     4921 aatgttttgt tgcgaaaaca atctaatcat aactaccgcg aatactcaat catctacaaa
     4981 atggattaaa tgtgcttatt taataattaa tttatatatt taatgcatta attcttaaca
     5041 ttaattgttc aataatattc accaaatcaa tatcaaaaaa aatcgcaaaa catataattc
     5101 aatacaaatc atcaggatag gttttgcaac gcgtgcattt tgtccccttt ttcctcgttg
     5161 attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg gtagttaagc
     5221 agaaattaat atcgcttact ttaaccaccg tagcacaatt agctaatttt acggatgcag
     5281 aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt tgtatccagt
     5341 aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat cgttcgcttt
     5401 atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag cggcatccag
     5461 cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt tttttatgcc
     5521 cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa gtcattattt
     5581 tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt gtgaaagaaa
     5641 aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa gtcggtttcc
     5701 ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag gttaaaaaca
     5761 gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg gccgaatccc
     5821 tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca atgcacatcg
     5881 ccaccaagct gcgcagcacc ctagacgagg tgatcgaacg cgctatctat atggtgaaac
     5941 gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga agatgccggg cgtacaccca
     6001 ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc accatcaaca
     6061 ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc agcggcctgt
     6121 atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac gacgatttag
     6181 gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag gtggaaggtg
     6241 caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc atcatggcga
     6301 ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccaa gagatatggc
     6361 gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac aaagccattg
     6421 ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg ctgaaaaacc
     6481 gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc cagctgaatc
     6541 tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg gggtataaag
     6601 aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg gacaaaaaag
     6661 gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag caagaagagc
     6721 cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat atcgccaccg
     6781 ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac ggtaacggtc
     6841 cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc gaactggtga
     6901 aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg gatattgtca
     6961 ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt gtcgagtcct
     7021 ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa gtcgaaaaag
     7081 agttgcaacg caaaactcaa cacaacgaaa acaacaagga aaccgtgtga tgtcgaagaa
     7141 ttaccatatt gccgtattgc cgggagacgg tattggtccg gaagtgatga cccaggcgct
     7201 gaaagtgctg aatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca gccattacga
     7261 tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcga cggttgaagg
     7321 ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggcccgaagt gggaacattt
     7381 accaccagac cagcaaccag aacgcggcgc gctgctgccg ctgcgtaagc acttcaaatt
     7441 attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct gtccgctgcg
     7501 tgcagacatt gccgctaacg gcttcgacat ccagtgcgtg cgcgaactga ccggcggcat
     7561 ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag cgtttgatac
     7621 cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg aatctgctcg
     7681 taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat cctctatttt
     7741 atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac tggcgcatat
     7801 gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg acgttctgct
     7861 gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca ctggctcgat
     7921 gggcatgttg ccttccgcca gcctgaacga gcaaggtttt ggactgtatg aaccggcggg
     7981 cggctcggca ccagatatcg caggcaaaaa catcgccaac ccgattgcac aaatcctgtc
     8041 gctggcactg ctgctgcgtt acagcctgga tgccgatgat gcggcttccg ccattgaacg
     8101 cgccattaac cgtgcattag aagaaggcat tcgcaccggg gatttagccc gtggcgctgc
     8161 cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag aaggggtgta
     8221 atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta cgaagccgaa
     8281 aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac ctcaccgcag
     8341 gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa aaccttcgcc
     8401 actatggatc acaacgtctc tacccagacc aaagacatta atgcctgtgg tgaaatggcg
     8461 cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga gctgtatgac
     8521 ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg cgtcaccttg
     8581 ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc gtttggcgca
     8641 ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacaca aaccctgaaa
     8701 cagggccgtg cgaagaccat gaaaattgaa gtccagggca aagccgcgcc gggcattacc
     8761 gcaaaagata tcgtgctggc aattatcggt aaaactggca gcgcaggcgg caccgggcat
     8821 gtggtggagt tttgcggcga agcaattcgt gatttaagca tggaaggtcg tatgaccctg
     8881 tgcaatatgg cgatcgaaat gggcgcaaaa gccgctctgg ttgctcctga cgaaaccacc
     8941 tttaactatg tcaaaggccg tctgcatgcg ccgaaaggca aagatttcga cgacgccgtt
     9001 gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt tgtcactctg
     9061 caagcagaag aaatttcacc gcaggtcacc tggggcacca atcccggcca ggtgatttct
     9121 gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg tgcgtcggca
     9181 gaaaaagcgc tggcctatat ggggctgaaa ccgggtattc cgctgaccga agtggctatc
     9241 gacaaagtgt ttatcggttc ctgtaccaac tctcgcattg aagatttacg cgcggcagcg
     9301 gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt tcccggctct
     9361 ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatatttat tgaagccggt
     9421 tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga ccgtctgaat
     9481 ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca ggggcgcggc
     9541 gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac cggacatttc
     9601 gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta tcaaacacac
     9661 aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca tcccgaaaca
     9721 gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg actggcgttt
     9781 tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc cgcagtatca
     9841 gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc gtgagcacgc
     9901 gccctgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt ttgctgacat
     9961 cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg atgcagaagt
    10021 ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg tggatctgga
    10081 agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg ccttccgccg
    10141 ccactgcatg atgaatggtc tggacagcat cggcctgacc ctgcagcacg acgacgctat
    10201 tgcgtcttat gaagcaaaac aacctgcgtt tatgaattag accccttgcc cggtcaaatg
    10261 accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg gttaaaaata
    10321 atgcgacaac agaaataatc gcaattaccc agtagacagc aaagtgcccc caactttgtg
    10381 caattgctcc ctgaataaca ccagccagaa ttaccccggt agagatagtg ttggtaaata
    10441 aggtggtagc tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac
    10501 ccgcaacaat gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc
    10561 tatgaaagaa aatcaatcca gtgtaaaaca gcactcctgc caccactgca atgaccatca
    10621 ttcgccgctt accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca
    10681 gtccagctgc cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aaatcgctac
    10741 tgatccacaa tggcatatca ataatgtaca tagtgttgca ggtccacatt aacgtcgagg
    10801 cgacaaataa catccgtacg ttactatcct gccagtcacc ttgcattgat aaagcatttt
    10861 ccgacggcag ttctacccgc gccacagaca gaagcataaa tgcaatcaat accagactga
    10921 gtgtgaatat cccggcggca atcgaaaaca tcactgtaaa gccgtaattc aacgccagca
    10981 taaaggccag cggtggaccg attacccatg ccagagaaag ctgcgcacgc atcaccgagc
    11041 taaacatcac cacttctcgc gccgagttat ccgcgtattc ccgcgccaga gcaaataact
    11101 gtggcattgc cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat
    11161 aatgacgatt aaatgcaaac agtagcgcat tgccaatagc catcaaacag caaaatataa
    11221 tcagttttcg ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga
    11281 tcccagcaat agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct
    11341 cacgactcag aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa
    11401 aagcgaccag cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc
    11461 agatcatacg ttcccttttt agcgcggcga gaataaaaaa aaccagcagg tataatctgc
    11521 tggcgggtga ttttacacca atactcagtc acacatgatg caggcaagtc aactttcaga
    11581 attgcggtca tcccactgca tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa
    11641 ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat
    11701 ggggcacctc cttgcttcat cgttaggaat tattgactta atatagggaa aataaaattg
    11761 ctgtcttttg cgcaggagtt ccccttttat gccatctgct cgtctgcaac aacagttcat
    11821 ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca acgctcaacg aactggcagc
    11881 gttattgagc tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc aggatcgcgg
    11941 ctggctgacg tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta
    12001 taccgggctg gcgcttcagc aacagcgggc ggaagacctg ctggagcagg atcgtatcga
    12061 ccaactagtg cagttggttg gcgacaaagc gactgtgcgg caaatgctgg tttctcatct
    12121 gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg
    12181 taatctgcta cctggcagcg cattgcgccg ttccgaaacc catattgccc ggcaaatctt
    12241 cagttcgcta acgcgcataa atgaggaaaa tggggaactg gaagcagaca tcgcccacca
    12301 ctggcagcaa atatcaccgc ttcactggcg tttctttttg cgtccaggag tccattttca
    12361 tcatggtcgt gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct
    12421 gccgctctat tcgcatatta cggacattgt gtcgccgacg ccctggacgc tggatatcca
    12481 tctcacgcag ccggaccgct ggttgccgtt actgctgggg caagttccgg cgatgatcct
    12541 gccgtgcgaa tgggaaaccc tcagtaactt tgccagccat cctattggca ccggtccgta
    12601 tgcggtgatt cgcaacagca ccaatcaact gaaaattcag gcattcgatg acttcttcgg
    12661 ttaccgggca ttaatcgacg aagttaacgt ctgggttctg ccggaaattg ccgacgagcc
    12721 agccggtggt ctgatgctaa aaggtccaca gggcgagaaa aaagagattg aaagccgcct
    12781 ggaggaaggt tgctactatt tactgttcga cagccgcacc catcgcgggg cgaatcagca
    12841 agtcagggac tgggtaagct atgtgctttc tccaactaat ctggtctatt tcgctgagga
    12901 acagtaccag caactgtggt tcccggctta tggactgctc ccccgttggc accatgcccg
    12961 caccataaag agcgaaaaac cggctggcct ggaaagcctc accctgacct tttatcagga
    13021 tcacagtgag catcgggtga ttgccgggat catgcagcag attctggcaa gtcaccaggt
    13081 cacgctggaa atcaaagaga tcagctacga tcagtggcat gaaggagaga tcgagagcga
    13141 tatctggctt aatagcgcca actttacgct gccgctggat ttttcgctgt tcgcgcactt
    13201 gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac tggcaagccg acgccgcccg
    13261 ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag caactggtcg ccagtaaagc
    13321 aatggtgccg cttatccacc actggctgat cattcaggga caacgcagta tgcgcggcct
    13381 gcgtatgaat accctcggct ggttcgattt taaatcagcg tggtttgcgc caccggatcc
    13441 atgattgctg gtagataaca aaatcactac actaacgccg ttctcaacgg ggtgccacgc
    13501 aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg gataacgccg gcgaagggat
    13561 ttgaggctcc ttctcaagtc ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat
    13621 gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt
    13681 atacctacga ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa aaagcttttg
    13741 aagccaactg taactgcgaa ctgaaactgg tggcgctgga agatggcgtt tcgcttctca
    13801 accgtctacg gatggaaggc aaaaatagta aagccgatgt ggtgctgggg ctggataaca
    13861 atctgttaga cgccgccagt aaaaccggac tatttgccaa aagcggtgtg gcagcggatg
    13921 ccgttaacgt tcccggcggc tggaataatg acactttcgt cccgtttgat tacggctatt
    13981 tcgccttcgt ctatgacaag aacaaactga aaaacccgcc gcaaagcctg aaagaattgg
    14041 ttgagagcga tcagaactgg cgggtgattt atcaggatcc gcgtaccagc acgccggggc
    14101 tgggtctgct gttgtggatg caaaaagtct atggcgatga cgccccacaa gcctggcaaa
    14161 aactggcgaa gaaaaccgtc acggtcacca aaggctggag cgaagcctac ggcctgtttt
    14221 taaaaggtga aagcgatctg gtactgagtt acaccacctc tccggcttat cacattctcg
    14281 aagagaaaaa agataactac gccgccgcga actttagcga aggtcactat ctgcaggtgg
    14341 aagtcgccgc ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa ttcctccagt
    14401 ttatggtttc tccggctttt cagaatgcga tcccaaccgg taactggatg tatccggtgg
    14461 caaatgtcac gctgcctgcc gggtttgaac aattgaccaa accggccacc acgctggagt
    14521 tcacaccagc tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg caacgcgccg
    14581 tcagccgtta attcccggct ggttaattcc aggtgtaagc gccgccacgc tggtggtagc
    14641 ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta actgggtggc
    14701 agtctggcag gacagctacc tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct
    14761 ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc cgcgccctct atcgcaggcg
    14821 ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct
    14881 agttgctgtt ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg catcgctctg
    14941 ccaatcgctc ggtctggagt ggaccttttc gccctacggc ctgcaaggca ttttgctggc
    15001 gcacgtattt tttaatctgc cgatggcgag ccgcttatta ctccaggcac tggaaaacat
    15061 tcccggcgaa caacgtcagc ttgccgccca gcttgggatg cgcggctggc attttttccg
    15121 cttcgtcgaa tggccgtggc tacggcgaca aatcccgccg gttgccgcgc ttatctttat
    15181 gctctgtttc gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc aggcaaccac
    15241 tatcgagctg gctatttatc aggcgctgag ttacgactac gatcctgccc gcgcggcgat
    15301 gctggcgctg ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc agcgactaag
    15361 taaggccatt gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg acgatcgtct
    15421 gcatagccgc atttgcgaca cggtgttaat agtgctggcg ctgctgctgt tgctgccacc
    15481 gttgctggcg gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc tggcacaacc
    15541 ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg
    15601 cgtagtgctg accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc ggcagaaaat
    15661 gctggcgggt caggcgctgg agatgagcgg catgttgatc ctcgccatgc cggggattgt
    15721 gctggctacc ggcttctttt tactgctcaa caacactatc ggcctgccac aatctgctga
    15781 cggcattgtg attttcacca atgcgttaat ggcgatccct tatgcgctga aagtactgga
    15841 aaacccgatg cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga
    15901 aggctggtcg cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc
    15961 gctggctttt gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa
    16021 cgatgatttc cgtaccctgc cgttttatct ctaccagcaa attggctcct atcgcagcca
    16081 ggacggcgcg gtcaccgcgt taattctgct actactctgt tttctgctgt ttaccgtgat
    16141 tgaaaaacta ccggggcgaa atgttaaaac tgactgatat cacctggctt tatcaccatt
    16201 tgccgatgcg ttttagctta acggtagaac gcggcgagca ggtggcaatc ctcgggccaa
    16261 gcggcgcggg taaaagtacc ctgctgaatt tgattgccgg ttttctgacg ccagccagcg
    16321 gttcgctgac tatcgatggt gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt
    16381 cgatgctgtt tcaggagaac aacctgttca gccatctgac ggtcgcgcag aacatcgggc
    16441 tggggctaaa tccggggttg aaactgaacg cggcacagca ggagaaaatg cacgcgattg
    16501 cccgtcagat ggggattgat aatttaatgg cgcggttacc gggcgagctt tccggcggtc
    16561 agcgacagcg cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg
    16621 atgaaccgtt ctctgcgctc gatccggcgt tacgtcagga gatgctgacg ctggtgagca
    16681 cgagctgcca gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg
    16741 cgcggatcgc cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga
    16801 ctgaagaatt gttgagcggt aaggcgagtg cttcggcaat attggggatt acgggttagt
    16861 atgcactgtc agatgcggcg tgaacacctt atccgaccta cggacagtgc gttttgtagg
    16921 cctgataaga cgcggtcagc gtcgcatgag gcaacccgca caagacaatc ccctaaaccc
    16981 caaccacttt acgcaaaata tcgatataca ccggcatcaa cgggtggcga attaacacca
    17041 ccagcgccac cacgccgatg gcagaaatca acggcgtcag ccacaacaaa cggccgcgcg
    17101 acaaataatg acttaaacga tcggttgctt taccgctgcg ccataaccgc cagcacagcc
    17161 agccaccaac ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac tcgccgctct
    17221 gcattccggc tggaatatcg atcgccgcgc ccgccagaat cccaggcagg aagtaaaacg
    17281 gtggccacag caggcagccg ataatattcg gcgtaatgaa ttttgccacc ggcagatcca
    17341 gcattcccgc caccattggc accagcggac gcgtcgggcc aacaaagcga ccgaccagaa
    17401 tggtgaacat gctgtgttga tgcaacgcat gttcagtttt atcgagtaat gctttgtttt
    17461 tcttcagaaa tgaccagcga tgcaacggct ttttaaaacg ccagcccagc cagaaggaga
    17521 tccagtcgcc cagcagacaa ccgacaatcc ctgccagcca agcgtgccag aaacttaact
    17581 cgccgctgcc aatcagcgct cccagccccg ccatcagcac cgtaccgggt agaatcaaac
    17641 cgaccagcgc cagcgactcc agaaaggcca ccaacaccac cgccatcaat gaatacacgg
    17701 tagattgggt aataaagtgt tccagcaatg cttgcataat gtgcctgtca aatggacgaa
    17761 gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc
    17821 aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg
    17881 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa
    17941 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc
    18001 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa
    18061 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa
    18121 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg attatccatc
    18181 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga
    18241 ttcatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca
    18301 aacaggtcgc tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc cgtattggca
    18361 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac
    18421 tggtgatacc attcgcgagc ctccggatga caaccgtagt gatgaatctc tcctggcggg
    18481 aacagcaaaa tatcacccgg tcggcagaca aattctcgtc cctgattttt caccaccccc
    18541 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa
    18601 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac
    18661 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca
    18721 ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac
    18781 tggctcttct cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc
    18841 aggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
    18901 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
    18961 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt
    19021 ttttggatgg agtgaaacga tggcgattgc aattggcctc gattttggca gtgattctgt
    19081 gcgagctttg gcggtggact gcgccagcgg tgaagagatc gccaccagcg tagagtggta
    19141 tccccgttgg caaaaagggc aattttgtga tgccccgaat aaccagttcc gtcatcatcc
    19201 gcgtgactac attgagtcaa tggaagcggc actgaaaacc gtgcttgcag agcttagcgt
    19261 cgaacagcgc gcagctgtgg tcgggattgg cgttgacagt accggctcga cgcccgcacc
    19321 gattgatgcc gacggtaacg tgctggcgct gcgcccggag tttgccgaaa acccgaacgc
    19381 gatgttcgta ttgtggaaag accacactgc ggttgaagaa gcggaagaga ttacccgttt
    19441 gtgccacgcg ccgggcaacg ttgactactc ccgctatatt ggcggtattt attccagcga
    19501 atggttctgg gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc
    19561 cgcatcgtgg attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc
    19621 gcaggatatt cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg
    19681 gggcggcttg ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt
    19741 gccttccccg ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc
    19801 ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg atttccggcg gcgcgtttga
    19861 ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac gcactggtaa aagttatcgg
    19921 tacttccacc tgcgacattc tgattgccga caaacagagc gttggcgagc gggcagttaa
    19981 aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga tttatcggtc tggaagcagg
    20041 ccaatcggcg tttggtgata tctacgcctg gtttggtcgc gtactcggct ggccgctgga
    20101 acagcttgcc gcccagcatc cggaactgaa agcgcaaatc aacgccagcc agaaacaact
    20161 gcttccggcg ctgaccgaag catgggccaa aaatccgtct ctggatcacc tgccggtggt
    20221 gctcgactgg tttaacggtc gtcgcacgcc aaacgctaac caacgcctga aaggggtgat
    20281 taccgatctt aacctcgcta ccgacactcc gctgctgttc ggcggtttga ttgctgccac
    20341 cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat caggggatcg ccgtcaataa
    20401 cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc attatgcagg cctgctgcga
    20461 cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag tgctgtgcgc tcggtgcggc
    20521 gatttttgct gccgtcgccg cgaaagtgca cgcagacatc ccatcagccc agcaaaaaat
    20581 ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa caggcacaac gctttgaaca
    20641 gctttatcgc cgctatcagc aatgggcgat gagcgccgaa caacactatc ttccaacttc
    20701 cgccccggca caggctgccc aggccgttgc gactctataa ggacacgata atggcgattt
    20761 ttgataatta tgaagtgtgg tttgtcattg gcagccagca tctgtatggc ccggaaaccc
    20821 tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc gctgaatacg gaagcgaaac
    20881 tgccctacaa actggtgttg aaaccgctgg gcaccacgcc ggatgaaatc accgctattt
    20941 gccgcgacgc gaattacgac gatcgttgcg ctggtctggt ggtgtggctg cacaccttct
    21001 ccccggccaa aatgtggatc aacggcctga ccatgctcaa caaaccgttg ctgcaattcc
    21061 acacccagtt caacgcggcg ctgccgtggg acagtatcga tatggacttt atgaacctga
    21121 accagactgc acatggcggt cgcgagttcg gcttcattgg cgcgcgtatg cgtcagcaac
    21181 atgccgtcgt taccggtcac tggcaggata aacaagccca tgagcgtatc ggctcctgga
    21241 tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa agtctgccga tttggcgata
    21301 acatgcgtga agtggcggtc accgatggcg ataaagttgc cgcacagatc aagttcggtt
    21361 tctccgtcaa tacctgggcg gttggctatc tggtgcaggt ggtgaactcc atcagcgacg
    21421 gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta caccatgacg cctgccacac
    21481 aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc gcgtattgag ctggggatga
    21541 agcgtttcct ggaacaaggt ggcttccacg cgttcaccac cacctttgaa gatttgcacg
    21601 gtctgaaaca gcttccaggt ctgcccgtac agcgtctgat gcagcagggt tacggctttg
    21661 cgggcgaagg cgactggaaa accgccgccc tgcttcgcat catgaaggtg atgtcaaccg
    21721 gtctgcaggg cggcacctcc tttatggagg actacaccta tcacttcgag aaaggtaatg
    21781 acctggtgct cggctcccat atgctggaag tctgcccgtc gattgccgta gaagagaaac
    21841 cgatcctcga cgttcagcat ctcggtattg gtggtaagga cgatcctgcc cgactgatct
    21901 tcaataccca aaccggtcca gcgattgtcg ccagcctgat tgatctcggc gatcgttacc
    21961 gtctgctggt taactgtatc gacacggtga aaacaccgca ctccctgccg aaactgccgg
    22021 tggcgaatgc actgtggaaa gcgcaaccgg atctgccaac tgcttccgaa gcgtggatcc
    22081 tcgctggtgg cgcgcaccat accgtcttca gccatgcgct gaacctcaac gatatgcgcc
    22141 agttcgccga gatgcacgac attgaaatca cggtgattga taacgacacc cgcctgccag
    22201 cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg atttcgtcgc taagtagcca
    22261 catcaggtat gtaacgcctg atgcgacgct gacgcgtctt atctggccta cacgctgcga
    22321 ttttgtaggc cggataagca aagcgcatcc ggcattcaac gcctgatgcg acgctggcgc
    22381 gtcttatcag gcctacgcgc tgcgattttg tagaccggat aagcgaagcg catccggcac
    22441 gaaggagtca acatgttaaa agatctcaaa cgccaggtat tagaagccaa cctggcgctg
    22501 ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga tcgcgagcgc
    22561 ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc tgacgatatg
    22621 gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc ctcctccgac
    22681 acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat tgtgcatacg
    22741 cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc aaccggcacc
    22801 acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac cgacgcagaa
    22861 atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt tgaaaaacag
    22921 ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc gtttgcatgg
    22981 ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt cgcttatatg
    23041 gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac gctgctggat
    23101 aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg actgtataaa
    23161 accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga tggattttca
    23221 gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg caagggacag
    23281 aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt gcaccgcaag
    23341 agtccgtggc gtttattcac gccgatcagg ttccccgcgc tcagcatatt ttgcagggtg
    23401 aacaaggctt tcgtctgaca ccgctggcgt taaaggattt tcaccgccag ccggtgtatg
    23461 gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgtctg cgtgaaggtg
    23521 gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg gagcggttta
    23581 tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt aatgcccgtc
    23641 tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat attgaaacca
    23701 cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc atcgtttata
    23761 tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa tacgtcgcca
    23821 gccgcccgct gctgctggaa aaactcaacg cctggtttgc aaaccacgat cccgatgtga
    23881 tcatcggctg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat gccgagcgtt
    23941 accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc gagcacggct
    24001 ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac ggtatcgagg
    24061 cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc gctcaggagc
    24121 tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa attgaccgcc
    24181 gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc gagctggtga
    24241 cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca acggtgaacg
    24301 gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc tattttccgc
    24361 gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg cacgccagcc
    24421 ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg gtgctggact
    24481 ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc gggctggttg
    24541 aaggcatggc gcaacctgat ccagagcaca gtaccgaagg ttttctcgat gcctggttct
    24601 cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg cgcgataaag
    24661 ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa gatcattatg aatgcctttt
    24721 atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcgcctggca tcgtcgatca
    24781 ccatgcgtgg tcatcagatc atgcggcaga ccaaagcatt gattgaagcg cagggctacg
    24841 acattatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca cattcggaag
    24901 aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg tgggcggaaa
    24961 cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact catttctgcc
    25021 gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc tacgccgggc
    25081 tgattcagga gggcgacaag cagcggatgg tgtttaaagg gctggaaacc gtgcgcaccg
    25141 actggacgcc gctggcccag cagtttcagc aggagctgta cctgcgtatc ttccgcaacg
    25201 agccatatca ggagtacgtc cgccaaacca tcgacaaact gatggcgggt gaactggatg
    25261 cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag cgtaatgttc
    25321 cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa tcaaaagcgc ggtcgcccct
    25381 tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc ccggagccgc
    25441 tggactacca acgttcacca ctggattacg aacactatct gacccgccag ctacaacccg
    25501 tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg accgggcaac
    25561 ttgggctatt ttgagcaaaa aaaagagttc gccagatacc attttgatgc gtgacgaatg
    25621 ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac accactacct
    25681 cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc gaacacatat
    25741 gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg gacttggaac
    25801 cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg gtgaaaaccg
    25861 tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg gtgataccat
    25921 taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa atggcttgct
    25981 gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc gtgaagtttt
    26041 ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt ctgtttgccg ggcagattga
    26101 ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg aacagttccg
    26161 tatgccgtac agcggcctgc gcggtcagcg taccagcctg atcccgcatc agctcaacat
    26221 cgctcatgat gttggtcgcc gccacgcgcc gcgcgtcctg ctggctgacg aagtgggttt
    26281 agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg gcgctgctga
    26341 acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag aaatgctgcg
    26401 ccgtttcaac ctgcgctttg ctctgtttga tgatgagcgt tatgccgaag ctcagcacga
    26461 tgcttacaac ccgtttgaca ccgaacagct ggtgatttgc tcgctggatt ttgcccgtcg
    26521 tagcaaacag cgcttggaac atctctgtga agccgaatgg gacctgctgg tggtcgatga
    26581 agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg ccattgaaca
    26641 actggcagag cacgtgccgg gcgttctgct gctgaccgcg accccggaac agctggggat
    26701 ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg attttgcgca
    26761 gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca tgctgctggc
    26821 aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg gcgagcagga
    26881 tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga gcgcccgtca
    26941 ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt tccgtaacac
    27001 gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaagc tgccgctacc
    27061 gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta aaagtgcgga
    27121 agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg aaggtgataa
    27181 cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc tgaccagcca
    27241 tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc aactggagca
    27301 ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta tgtcgattat
    27361 cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac aggtactgct
    27421 gtgctcagaa atcggttctg aaggacgtaa ctttcagttc gccagtcaca tggtgatgtt
    27481 tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg atcgtatcgg
    27541 ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc agtcggtgct
    27601 ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga ccggacgcac
    27661 tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg atcaaaccga
    27721 aggctttgac gatctgatca aaagctgccg cgagcaacat gaagcgctga aagcacagct
    27781 ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa aagcccaggc
    27841 actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct tcgccatgaa
    27901 cctgttcgat attatcggta tcaatcagga cgatcgcggc gacaacatga tcgtgctgac
    27961 gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg gcatcaccat
    28021 cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta cctgggagca
    28081 tccgctgatc cgcaacggtc tggatctgat cctttctggc gataccggta gcagcacgat
    28141 ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac tgatttatgt
    28201 ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac cgacgccggt
    28261 acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt ttgaaacctt
    28321 taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta acgccgtgca
    28381 gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat ctgcccgtgc
    28441 attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc tgtctcgtct
    28501 ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg ccattgagag
    28561 caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg atgccctgcg
    28621 tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa ctacaatcca
    28681 ccgcaggaac cctggttggt tatcctgtat caggatgacc atattatggt ggtcaacaag
    28741 ccgagcggtt tgttgtcagt gccgggtcgt ctggaagagc acaaagacag cgtgatgacg
    28801 cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat ggctaccagc
    28861 ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg ccagttccgc
    28921 gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc ccccgcagaa
    28981 ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca gaaagtctgt
    29041 tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc ggcggataac
    29101 acggcaagag tggtgttaaa accgattacc gggcgttcgc atcaattgcg tgtgcatatg
    29161 ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga agcgagagcg
    29221 atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc ggcgtatggc
    29281 aatagtatga cgtttaaagc gccagcggat ttttaatcgt cacgctggcg cagttcggta
    29341 ggcggataag gcgttcacgc cgcatccggc aattgtgcgc cgattgcctg atgcgacgcg
    29401 ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg ctgctttatc
    29461 agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat catctcaggc
    29521 ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt acggtaggca
    29581 cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca ggcatcttcc
    29641 agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg ctgataaccg
    29701 ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag
    29761 atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg cagtgaacca
    29821 tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc aaaacgacca
    29881 aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattatc tgattttccc
    29941 acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat tcggtccatc
    30001 aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt ggttaaatgc
    30061 cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg gttggcgaac
    30121 cacgccattt tacggctacg ggctttatca aacatatggc caattaacag gcctaacact
    30181 acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat ttttccccaa
    30241 tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca caatataaag
    30301 gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa tttgcattat
    30361 catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca ggattaacac
    30421 tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat
    30481 gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg ccaccatgat
    30541 tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac agtgcatgtt
    30601 gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact tacccgtgac
    30661 tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta ctggcagcgt
    30721 ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga
    30781 ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg tccattacga
    30841 cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca ccaaagatac
    30901 caacgtctgg gaaggtgatt accagatggt gggtcgccag ggtcgcggta aagcggacct
    30961 gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct ttacctcctg
    31021 tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg accgcgaaga
    31081 acaagttgct gagatctgga acgcccgctt taaggtgggt ccggtaccga tcttttatag
    31141 cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc
    31201 caagtacacc accaccaact actttgagtt ctacctgcca tattactgga acatcgcgcc
    31261 aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga
    31321 gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg actatctgcc
    31381 ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc gttggttatt
    31441 ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg actacaccaa
    31501 ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca gtactgacgg
    31561 ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc
    31621 aaccaagcag ttccaggttt tcagcgaaca gaacaccagt agctactcgg cagagccgca
    31681 gttagacgtt aattactacc agaatgatgt tggtccgttt gatacgcgta tttacggcca
    31741 ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg ttcacctgga
    31801 accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg aagcgaagtt
    31861 tctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa acacgaccaa
    31921 gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca aaatggtctt
    31981 tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac cgcgcgcgca
    32041 gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact cgtctctgct
    32101 gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg accgtattgc
    32161 ctccgctaac caagtgacga ccggtgtcac atctcgcata tatgatgatg ctgccgttga
    32221 acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca ctggcgatga
    32281 caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag gcgacaccta
    32341 ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata cacgtctgga
    32401 taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc gtctggtaca
    32461 gctgaattac cattacgcca gcccggaata tattcaggct acgctgccta agtactattc
    32521 cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca gcaggccaat
    32581 tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga acaagcaagc
    32641 cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg tcggttacga
    32701 gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca acgcaatcgg
    32761 ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc aagagatgct
    32821 gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac cacgtaatcc
    32881 gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg cttctcggta
    32941 tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa gtcgcagccg
    33001 tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag tcggtaaaac
    33061 tgaacgctgc gcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc caccaaatca
    33121 tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa
    33181 tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac aacatgacgc
    33241 tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacact tatcgtaacc
    33301 agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca
    33361 ccatcctgcc acaggaagtc gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca
    33421 gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg acctctgatc
    33481 aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg
    33541 atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac ggcggccaga
    33601 tggggtgggg ccgtattcag gagttgccgg ggatcttcgc ccaggcatta agcaccgcga
    33661 agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta
    33721 acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct cgccatattc
    33781 tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg gagcaaattg
    33841 ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag ttctcgcagg
    33901 atccaggttc tgctaaccag ggcggtgatc tcggctgggc tacaccagat attttcgatc
    33961 cagccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca ccggttcact
    34021 cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat aaaaccgacg
    34081 ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg gaagaagcag
    34141 caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc aactaatggt
    34201 taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc cggacttagt
    34261 tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg atgccactct
    34321 ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt attcccccaa
    34381 ctcccctgca caaccgcaaa ctacgggcac attaacgcta cttcctgtcg cgctacgtga
    34441 atctgtcact gcggggcagt tagcgattga aaatgggcat tacgtggtgg agacgctggc
    34501 gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag gccccgtgca
    34561 taaaggcgtc attaacgacg caagcattcc gtttaccggt cataccgagt ttttcgaaga
    34621 gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc gcgtggcgct
    34681 ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg cacttttgca
    34741 cgaagtgatt gctattttgc atcacgattt gcgtacaaaa tttggtattg ccgaaccgcg
    34801 cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg gtacggaaga
    34861 gatagacacc attattccgg tgctcgacga gctgcgggcg caggggatga aactcaacgg
    34921 gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg acgccgtgct
    34981 ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt
    35041 gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca ccgcgcttga
    35101 actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta atctcgccat
    35161 caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt agcccgtaaa
    35221 cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt ttctgccatt
    35281 aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtctggcggc attgaccgag
    35341 ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga tctggcggca
    35401 cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca ggatgcgatg
    35461 acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac
    35521 ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac tgatgccatt
    35581 gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac
    35641 agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt gatcccggta
    35701 ctggaagttc cgccgtcagc ctttacacca ccacccaaag tggattccgc cgtcgtgcgc
    35761 ctggtccctc atgcaacgat gcctcacccg gttaaagata ttcgcgtgtt gagccgcatc
    35821 accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg caacctgttt
    35881 agcgtcgagg tgttaaccgg aatggggatc gacccggcga tgcgagcgga aaatatctct
    35941 gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt gcaggagagt
    36001 taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac attgaggcac
    36061 aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata cgcaatctgg
    36121 ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc aatggccgtg
    36181 aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg cctggcgaag
    36241 agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg cagggtcact
    36301 acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta ttccgactcg
    36361 ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc gacgttcatg
    36421 gttgttacga tgaactgatc gcattgctgc ataaagtaga atttaccccc gggaaagata
    36481 ccctctggct gacgggcgat ctggtcgcgc gcggcccggg ttcgctggat gttctgcgct
    36541 atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat ctgcatctgc
    36601 tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg
    36661 aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctctg ctgcaaatcg
    36721 acgaagagaa aaagctggtg atggcccacg cagggatcac gccgcagtgg gatctgcaga
    36781 ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc gagtgactcc tatcccttct
    36841 ttcttgacgc tatgtacggc gatatgccaa ataactggtc accggaattg cgggggctgg
    36901 gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttc ccgaacggtc
    36961 aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg aaaccgtggt
    37021 ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac tgggcatcgc
    37081 tggaaggcaa aggtacgccg gaaggtattt acgcgctgga taccggctgc tgctggggtg
    37141 ggtcattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg tcgaaccggc
    37201 ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatacagga aggccggata
    37261 agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaact taccgccgct
    37321 ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc ggcgtcgtgg aattcgctga
    37381 ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg
    37441 cgtcgatatg cgtcagatac agtttttgcg cttttggcag gaactgctca taaacgcgac
    37501 cgccgccaat caccatgatt tctggtacgt caccacacgc cgcaatggct tcatccaccg
    37561 atttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata atatttttgc
    37621 gtcctggcaa cggacgaccg attgattccc aagtatggcg gcccataatc acgggtttat
    37681 ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc atggcgtttt
    37741 ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt gagatttccc
    37801 gataaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg gtgcgaggat
    37861 gatgttgagg aaaattttat attctgctgg cgagtccacg ctctccccct ggactcgccg
    37921 cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt cactctttat
    37981 taagatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt accttcttct
    38041 gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc ggtgatgatc
    38101 tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt gtcattctca
    38161 accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc ggcacgttct
    38221 cgcgcttcat acggccccag acctaaactt tccagcgcca gacgcccggt tttcagcgca
    38281 ccttcaaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg aatgtagtga
    38341 tcgacatcgc gggcgcgggc aataatctgc aaatgcggga aatgttcttt caccatctcc
    38401 gtcagttgca ggttggtttg cggatcgtca atagcgttaa tcagcacttc cgctttcgcc
    38461 gctccggcag attccagtaa atccatccgc gtggcatcgc cataaaacac tttcatacca
    38521 aatttacgca gcgtttcgat atggtccgga tcgtgatcga gcaccaccat tttcaccccg
    38581 ctggagagca gtaaacgtcc ggtaatctgt ccaaaacgac cgaatccggc gataatcacg
    38641 cgcggctgtt cttcatcgat ctcatcggct tcacgcgcct cctccgtaga agattgctca
    38701 aggcgattga ggatcaccag cagaatcggc gttgctgcca tcgacagcgc caccgccagg
    38761 gtcagcgatt tcgcccactc cggctccagc acattcgcca tctgcgccgc accaaatacc
    38821 acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accagcgacg ctgtttattt
    38881 ggcacctgca acggtcgggc aatcagccac agcatggcga ttttgatgat gaggaaaccg
    38941 agcagcaaaa tgacaatgcg caatgggttt tcaatcagcg tgccaaagtc tatcgacatg
    39001 ccaacaccga tgaaaaacag ccccaacagc aaacctttaa acggttcgat atcgctttcc
    39061 aacgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc gcccatcgcc
    39121 atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacaaggaa taacgccacg
    39181 gcactaaaca cttcccgcaa gccagagcgg gcaacaaaac gcagcgccgg acgcgtaaca
    39241 tagcgcccca gcaataccac cagtaccagc gcacccgcca cttttaacgc cgagagagca
    39301 aatgcgccca tcgtcgtcga ggcactgctc gccgccagta gcgggatcat cgccaccagc
    39361 gggattgccg cgatatcctg gaacagcagc actgcaaagg cactgcggcc catttgcgtc
    39421 accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgttgatga gagcgccagc
    39481 gtcataccga tcaattccgc gacctgccag cgcagcccaa gtaacataca gaacagcccc
    39541 agcaggccac cgcaaatcac catctgcaat gcgccaccgc cgaacactgc cgcacgcagc
    39601 ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag caccacccca
    39661 atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc ccacgggcca
    39721 ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg taccgcaatg
    39781 ggtacaatca gcgccgccga tccgaggtag atcagcgcct gaaccagcgt atgactatcc
    39841 atgatgggcc tcctgccatt ccagcagacg ttgcttatag tggcgcgcct gcccttcgag
    39901 ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc agttcagccc
    39961 gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg gatgcgcacc
    40021 aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat gtttgccatg
    40081 cagcgccgtg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat cgatccaaag
    40141 tttaagaagc ggaggaatgc tgtaccactg catcgggtgc tgccagacga tcagatcggc
    40201 gcgagagagc gcctcctgct cggcggcaat atcgatattg aagtcaggat agagttgata
    40261 aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc gtttattcgc
    40321 atgggaatga tgcggatacg gatgcgcata aatgatgagg atcatagtta ttagcccgcg
    40381 tgtttacgcc tttgttttcc cacaaagtgt agtcatatat tcacgcggct tatagtcaat
    40441 aatattgatt cagaaaagag agaaatctga acaaataatc ccattctctg cccgcggatc
    40501 gtcatattgc tggtctgcgc cagtgacatt cctcgagtct ccggggcaaa cgctacggaa
    40561 atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact gataccgtaa
    40621 ttattgataa atatcggcag tgcccaggtc gaaacaatgg tgccaatacg acttaaggac
    40681 ataatcacgc ccacggcaga ggcgcggata tccgtcggga agagttcatt aggatagagc
    40741 cactgcaaat tacccggccc gccagagaaa aaggcataca ccgcaaaggc catcactacc
    40801 agccagatcc ccatatccgg gattagcccc aaaaccgcca acgccagcgt catcatggca
    40861 aagctgccaa tcaacaatgg acgccgtccg gcagtgttta gccacagcat cggcggaata
    40921 cagccgagca taaagaacag gctaatcacc acattcccta gtgccgcgtt tttgccaacc
    40981 cccaatccca acgaaccaac gatttgcggg ccaaaggtgt aaatggcgaa cattgggatc
    41041 acctggcagg tccagatggc ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac
    41101 agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac
    41161 agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa ccagcgggga
    41221 gattctggca gttcgaatcg accaatcaaa atcaacaaac aagggatcgc cgcgctaccc
    41281 agcatccagc gccaaccacc ttccacatca taaagccagt agccgaccag atcggcgcag
    41341 gtcgcgccga cataccacat cgcggcaata aagctgatgg aaaacgcccg ctgacgggta
    41401 ctggagaact cggtgatcat cgaagtggcg atgggataat ctgcaccgat gacaatgccg
    41461 atgagtaccc gcatcaccaa cagttcgacg ggggatgaaa cacacatcgt cgccaccgat
    41521 atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac tttatcggag
    41581 atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc gcccagcaag
    41641 ccaatccagt cagcgtccag tttcagcgcc ggagtcagtt gctccagcgc cacgccaatc
    41701 attaccagta cataaccatc cagaaacgga ccaccgcttc cccacagcaa aatgcggcgg
    41761 tgaatagagg agaatttgag atcgtcaaag tttctggacg gttgcatggg ttattcctgt
    41821 ttttttgcaa tgagagtcag tacggcgtta cgtatcaaac caacatcagc cgtaacggaa
    41881 ctccacacca aaggtgccgc gcgggtattc ccactgttcc agcgccgacc ccagccccag
    41941 aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc
    42001 ctgcttcttg tacagacctg cggggcacgc tttcaccagc agctccagca cctgtttatc
    42061 agcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg acattgaatt tattgacgcc
    42121 cagtttgacg tccacattga cgggagaagt cataaaacgg tcactccttt catgccatcc
    42181 ttgatcagat tgatgaagcc cactttcttg ccgtggcgga ggattttctt gcgcatcagt
    42241 tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca cgcccaccgc cagttccggg
    42301 ttgccgctaa acatgcgtgg gttatcaagg aacgccggta gtttctggta catgcgcata
    42361 tcgcgcagcg ggccactctc cagatgctga cgatattccg cgagtttttg cttactgaaa
    42421 tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag cttccccggc ggcaatcgcc
    42481 agatccatac cgcgaatggt aaaaccgagg ttcatacaca ttccggcggc atcaccggca
    42541 atcaatacgc cgtcaccgac cagctccggc agcatgttga tgcctgcttc cggcactacg
    42601 tgagcggaat attccaccag cttgccgccc gcgatcagcg gtgcaacggc cggatgctgt
    42661 ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt catgcagatg atgcagacca
    42721 caaaccagcc ccagcgacag ggtattttca ttggtataaa ggaagccgcc gcccatcagg
    42781 ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct gattaccctg caactgaaaa
    42841 cggtcttcga ttaccgactt cggtaactcg atcagttcct tcacgccaac cgccacatcc
    42901 gtcggtttaa cgcgttttgc catccccaac ttttcggcaa ggatggagtt caccccatca
    42961 gcaaggagca ccgttttcgc ttcaatcaca tcaccatcgg cttctacacc gacgactttg
    43021 ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa ttaactgcgc gcccgcttct
    43081 tcggcctgct ccatcaacca ggcatcaaat ttactgcgca aaacggagta agaacgctgg
    43141 gatggcgagg tttcgtcacc gttgcagtag tccatagtca tcgctgactt ttccgtcata
    43201 aacgcgagtt tttcatgggt gatcaggcgt tctacggggg cggaatcggc gaaacctgga
    43261 ataatgtgtt ccagactgtg ggcatagaga cgcccgccag taacgttctt ggcacctgcg
    43321 gaattgccac gctcgataac taacacttgc gccccttcgc gggcgagcac cagtgcggca
    43381 accgaaccgg caagccctgc accgacgatg atggcgtcaa agatatcttc ggacataaga
    43441 gctccctgtc agcggcagag ccgtaatgca gccctgccag agtggatcaa cgcgctaaag
    43501 ctgcggtcag tgccggaagg atcttcacgg catcgccaac aatgccgtaa tccgcgtact
    43561 ggaagatcgg cgcattttta tctttattga tggcgaaaat ggtttgcgac gcgttagcgc
    43621 caaccatgtg ctggatctac ccggagatcc ccaccgccag gtacagttca ggtttcagca
    43681 tcaggttgga gataccgaca tagcgttcgt gctccatcca tttttcgttt tctgccaccg
    43741 gacgagaaca ggccaactcc gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt
    43801 tctctttgct gccaatgccg cgaccgacgc tgaccaccag acgggctttg tcgagatcga
    43861 cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc
    43921 actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc
    43981 cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg ttcttcgcca atcgccagac
    44041 cgccgtaaac catgtgtttc actgttgctt taccgtcctg tacgctgacg gtgctggcat
    44101 cgttagacac cgccgcttta aggcgataac ccagttttgc cgccagtaat ttgccgcgac
    44161 gggtgtttgg caacagcacc agaccgtcag cgccgtgctg gcgaatagtg tcagccatga
    44221 caccggcgta atcttcgatc atccgatcgt ccggtttgcc gtttaatttc cagacatgat
    44281 tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt gaggacaaag gcattgattt
    44341 gattagctaa agcctgcgca ccgttcatca gttccggcag acgagagggg gtatcgctga
    44401 atacccagac ttgagaaaac gtgttcatag catcccctgt aattaaatga ctttgcgaag
    44461 attttcagca aatgcggcga tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg
    44521 ctcgcgctgt ttcggcgcgg caacctgttg ttcagaccag gcgtccacgg cgttaaaacc
    44581 aatatccgcc gccgaccata cctggactgg ctttttcgcc gcgccgagaa tggctttcat
    44641 cgaaggaatt tgtggggagt tgatatcagt ggaaacagca acaaccgcag gcagcggaat
    44701 gcttaaggtt tcggtttcat cttccagttc gcgctcaacg gtgagggtat ctgccgtcag
    44761 ggagataatt ttgctgacgc cgttaactgc cggaatattg aggatttcgc ccaccagcag
    44821 accaacctgc tgggcataaa ggtcggaaga accatcgcca cagaggatca gatcaaagcc
    44881 tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa
    44941 ctggtcatca atcaccacaa tcagttcatc cgggccgcgc gatagcacat ctttacgccc
    45001 tttggcgttg gtcagggctt taccgcccac acttaaggct gtcacctgcg cctctgctgc
    45061 ctgttgcttt agctggcaag ccgcttcaat agcgttgaga tcgtattggc ttattttggc
    45121 atcggctttg ctgaagtcta atgaaccatc agcattattg accgcaatat cctgttcatc
    45181 aggcacgcac ttatagcaag taataatctt cattgcatct ccagaaatca tgaaggaaac
    45241 gtcgttcata tcggtggtgt taattacaga tatcacgccc tttaatttgg aaataaaact
    45301 ttaatcacca atattgaaag tcagatgcta tttttttacc cacaaaagaa tataacaaac
    45361 tgattaataa ccaaaattaa cgaaaaacgc cctctgttca ctcgaaacaa aaatgtgata
    45421 ccaatcacag aatacagctt attgaatacc cattatgagt tagccattaa cgcgtccacg
    45481 atgttaataa taattatatt aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa
    45541 atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga
    45601 accgaaggtt ttctttccgc cattaataat cgtcggcata ctttgttggc ttacagtcag
    45661 agatctggat gcggcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg
    45721 gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct ggctggtgtt
    45781 tggcccgtat gccaaaaagc gtttaggtaa cgaaccgcca gaatttagca ccgccagttg
    45841 gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg gatcgattga
    45901 gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga caggagcgaa
    45961 agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg ccacttacag
    46021 cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag tgattcgccc
    46081 cagctcgaca ctggtgccgc tggcaggtga aaaacacgcc aaagggttgt tcggtaccat
    46141 cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc
    46201 cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata ccctgcaact
    46261 ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg cttgcggtct
    46321 gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg
    46381 ttgggtgttc atcgtcagcg gtgccagctt catcatgaac tacttcaccg attcggtggg
    46441 gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg
    46501 cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttacg ccatccagat
    46561 gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat
    46621 ggtgatgggg ctgacagcat ccacctggat cctgtggacc gtgctcggta gtaacactct
    46681 gctgttgatg gataaaaaca tcatcaacat tccaaatctg atcgaacagt acggtgtggc
    46741 gcgcgccatc atcgaaacct gggccgctct gccactcagc accgccacca tgtggggctt
    46801 cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt ataccctggc
    46861 gatgtctact tgccgcgaag tacgcgatgg tgaagaacca ccgctgctgg tgcgtatcgg
    46921 ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc
    46981 gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac
    47041 gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc ccaaaatatc
    47101 aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg tcgccggtat
    47161 ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg accgtgacag
    47221 cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca gtctgctgat
    47281 ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg tgtggatgga
    47341 gctgggacgt ctgggggcac caacctatgt actgtaccag ctgccgggcg ggttcaatac
    47401 cttcctgcgc gaaggcacac aagagcagat cgacaagatt atggctttcc gcggcaccgg
    47461 taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg tgggtagcct
    47521 gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta agtgttttat
    47581 taccagtagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga
    47641 caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa
    47701 acttgagaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg acgatgtgga
    47761 actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga
    47821 gttcgaccat gaacgtttcc tggtagccct gaccaactac ggtacggcga tgtgcgcctt
    47881 tgaagatgct gcgcgctacg ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt
    47941 ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat
    48001 gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg atgcagcgat
    48061 gtgcaaatac ttctgcgcca atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct
    48121 gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt
    48181 agaccgcgtt tccggaggat ctgacgaaat gcagatcctg acgctgggtc gtgcggtgct
    48241 gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc
    48301 tacgggagca cgaatgtaag ccggataagg cgtttacgcc gcatccggca gtcatgcgct
    48361 acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt cggaccgctg
    48421 gccggattgc gcgttgtctt ctccggtatc gaaatcgccg gaccgtttgc cggacaaatg
    48481 ttcgcagaat ggggcgcgga agttatttgg atcgagaacg tcgcctgggc cgacaccatt
    48541 cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat
    48601 attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc
    48661 atcgaagcca gtaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg
    48721 cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta cggcaccgag
    48781 gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg ttacctgatt
    48841 cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga ttacttttct
    48901 ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga aaccggtaaa
    48961 ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca gtacttcatg
    49021 atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac
    49081 tacgccggtt gcggtctgta taaatgtgcc gacggctaca tcgtgatgga actggtgggc
    49141 attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact cggtacgccg
    49201 gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg cccactggta
    49261 gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa agaacgcttt
    49321 gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga aagcaatcca
    49381 cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg cacctgcaaa
    49441 gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg cggaatgccc
    49501 tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga aaacgacatt
    49561 caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg
    49621 cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag gtgcaatgga
    49681 tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg tttacggtca
    49741 taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata gttatcttga
    49801 gttaaatcag gagattaacc gcacggcaaa cctgttttat acgctgggga ttcgcaaagg
    49861 caacaaggtt gcactacatc tcgacaactg cccggaattt atcttttgct ggttcgggct
    49921 ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttacgcg aggaaagcga
    49981 gtggatcttg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat tttatcctat
    50041 gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc tgacagatgt
    50101 ggcacttccc gctgatgatg gagtgagttc atttactcaa ctgaaaaatc aacaacctgc
    50161 caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc tcttcacctc
    50221 cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc gcttcgctgg
    50281 atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacatga cggtaatgcc
    50341 tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg ccggggccac
    50401 ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggcgc agaagtaccg
    50461 cgccaccatt accgaatgta ttccgatgat gatccgtacg ttgatggtgc agccgccttc
    50521 agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact tgtcggagca
    50581 ggaaaaagac gcattttgtg aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac
    50641 ggaaaccatt gtgggcatta tcggcgatcg ccctagcgat aaacgacgct ggccgtcgat
    50701 tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc gcccgctccc
    50761 ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca tcttcaaaga
    50821 gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct ggctacatac
    50881 cggcgatacc ggataccgcg acgaagaagg ctttttttat ttcgtcgatc gccgctgcaa
    50941 tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata tcatcgccac
    51001 ccatccgaaa attcaggaca tcgtggttgt gggtattaaa gattcgattc gcgatgaagc
    51061 catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg aattttttcg
    51121 cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga tcagaaaaga
    51181 tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa tgaaacgtca
    51241 gggaactacc ctgccagcta ataatcacgc cctaaaacaa tatgcatttt ttgcagggat
    51301 gttgtcgtcc ctaaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac
    51361 ccgcaatgga tcaattctgg aaattaccct tgatcgccca aaagcgaatg ctattgatgc
    51421 aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg
    51481 tgtcgccatt attaccggtg ccggagagaa attcttttcc gcgggctggg atttaaaagc
    51541 ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggttttg cgggattaac
    51601 cgaaattttc aatctcgaca aaccagttat cgcagctgtg aacggctacg cctttggcgg
    51661 cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg ccagcttcgc
    51721 cctgccggaa gccaaactgg gcatcgttcc tgacagcggc ggtgtgctgc gtctgccgaa
    51781 gatcctgccg cctaccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga
    51841 agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac tgatggataa
    51901 cgcccgcgaa ctggctcagc agctggttaa cagcgccccg ctggcgattg cggcgctgaa
    51961 agagatctac cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag
    52021 cggcgtgttg aaacactatc catcggttct gcattcggaa gatgccattg aagggccgct
    52081 ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt gagctattac
    52141 gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc
    52201 ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc ctcactgcgt
    52261 ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg
    52321 catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcatggagcg
    52381 atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag cgtaattatg
    52441 gatggcgcgg tcattggcga agagagcatt gttgccgcaa tgagctttgt caaagcaggc
    52501 tttagtggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt
    52561 agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga tcttgtcggg
    52621 cgctgtcatg catcgttaca tgaaacgcag ccactgaggc aaatggagga aaatcgcccc
    52681 cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat gcgacctggt
    52741 tgctggataa gatgctacaa catcgtatct ggcaagcagg acgcccggta tcaacgagat
    52801 aaaattaacg acgcatactc tttgacaaca tcatctgcca cttctgctcg cggttaagtt
    52861 ccgtgagcgg aacggcagga atacgcggtg tctttcttac gccgactaat ttttcccgac
    52921 tgttattgcg caggcgcgcg taaatttgct catcgatatc gaccacttta accagtcgct
    52981 gacactggca tccccgccct tccagcttat tagggatcat cttaacttcg cagcttattt
    53041 ctgtgacttc cgacagaata taagtcaggg tattaaccgc cttgctgtgt tcaatatcga
    53101 aatgaataga gatctctctt gctatcaccc accgattttc agccatcatc cattcggcga
    53161 ttaacaagta gagtggtttt tcaacatatc cttcacacat acgaactcca gtttcacaaa
    53221 ttaaaagtct agctatgact cgttttgaag caattaaaca aggccatatt aaaattgtgg
    53281 atatatcgat agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca
    53341 tcaaaaatat cgattcccca aaagatttac ttaatgggca aaaaaaaacg gtcctcatca
    53401 gagaaccgta ttagttattc gatcaaatta gcggatgaaa aatatccgcc acgacacgct
    53461 attatttgat ctgtgcgtgc atttcctgca ccgaaattac tttttcagtc gcatcggcat
    53521 tcagcgccat cgcggtagcg aaaccaccgt tcagggtggt gtcataatgc actttatatt
    53581 gcagcgcact gcgacggatc acgcgggagt cttcaatcgc acgacggcct gaggtggtgt
    53641 tgatgatgta ggtatattcg ccattcttga tacggtcctg aatgtgcgga cggccttcat
    53701 gcaccttgtt taccagacgc ggattgatac ccgcttcgcc cagcacaatc gccgtgccgt
    53761 gggtcgcatc cagctcgaag ccctgtttca gcagttttgc cgccaggtcc accacgcgtt
    53821 ctttatcgcc ttcgcgcacg gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc
    53881 tgcccagctg cgctttggcg aacgcttcag cgaaggtgcg gcccacgccc atgacttccc
    53941 cggtagagcg catttctggc cctaacagcg ggtcaacgcc tgggaattta ttgaacggca
    54001 gcaccacttc tttcaccgag tagtacggcg gaataacttc tttggttacg ccctgctcag
    54061 ccagcgattt gccagccatc acacgcgccg ccacttttgc cagcggtacg ccggtggctt
    54121 tggagacgaa cggaacagta cgcgccgcac gcgggtttac ttcaatcagg taaacttcgt
    54181 tgtttttcac cgcaaactgc acgttcatca gaccgcgcac ctgcaattcg aaggccagtt
    54241 tctgcacctg ctggcgcatc acatcctgaa tttcctgact taaggtgtac gctggcagcg
    54301 aacatgcgga gtcaccggag tgcacacccg cctgctcgat gtgctccatg atgccgccaa
    54361 tcagcaccat ttcgccgtcg cagatggcat ccacgtcaac ttctactgcg tcatcaagga
    54421 aatgatccag caacactggc gcatcgttag acacgctaac cgccgtctgg aagtagcgac
    54481 gcaggtcagc ttcgtcatag acgatttcca tcgcccgacc gccgagaacg taagacggac
    54541 gcaccaccag cgggtagcca atctcttttg ccttctcaac tgccatttca atagcagtaa
    54601 cggtggcgtt cgccggttgt ttcagtttca gacggtcaac cgcatgctgg aagcgttcac
    54661 ggtcttccgc acggtcgata gcatccgggc tggtgccgat aaccggtacg ccagcggctt
    54721 ccagtgcacg cgccagtttc agcggggtct gaccgccgta ctgaacgata acgcctttcg
    54781 gcttctcaat acgcacgatt tccagcacat cttccagagt taccggctcg aagtagaggc
    54841 gatcggaagt gtcgtagtcg gtagagacgg tttccgggtt acagttaacc ataatggttt
    54901 cgtaaccgtc ttcacgcagc gccagcgagg cgtgtacgca gcagtagtcg aattcgatac
    54961 cctgaccgat acggtttgga ccgccgccaa gcaccatgat tttttcacgg tcggtagacg
    55021 gattcgcttc gcactcttct tcataagtgg agtacatgta agcggtgtcg gtggcaaact
    55081 ctgccgcaca ggtatccacg cgcttgtaga ccgggtgcag gtcatattgg tcacgcagct
    55141 tacggatttc cgcttcgcgt acgcccgcca gttttgccaa gcgcgcatcg gcaaagcctt
    55201 tgcgtttcag ctggcgcagg aattcagcgt tcaggccagt gatgcccact tccgccactt
    55261 tctcttccag acgcaccagt tcttcaatct gtaccaggaa ccagcggtca atgttagtca
    55321 ggttgaagac gccgtccaca gacaggcccg cgcggaaagc atcggcgatg taccagatac
    55381 gctctgcgcc tgcgtctttc agttcgcgac ggattttggt taacgcttcc gggtcatcca
    55441 ggctcacttt cgggtcgaag ccagttgcgc ccacttccag gccgcgcagc gctttttgca
    55501 gggattcctg ctgcgtgcga ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg
    55561 tcagacggtc gttagcaccg gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca
    55621 catagtcgat ggacggctcg aaggaggccg gagtacgtcc gccagtgatg tcgttcatca
    55681 gttcgtctag ggtgtaaccc accgccagtt tcgccgccac tttagcaatc gggaaaccgg
    55741 tcgctttcga cgccagcgcc gaagaacggg acacgcgtgg gttcatttcg ataacaatca
    55801 ggcgaccgtt tttcgggttc accgcaaact ggacattgga accaccggtt tcaacgccga
    55861 tttcacgcag caccgccatc gaggcgttac gcatgatttg atattctttg tcggtcagcg
    55921 tttgggctgg cgcgacagtg atggagtcac cggtgtggat gcccatcgca tcaaagtttt
    55981 cgatagagca gacgatgatg cagttgtcgt ttttatcacg caccacttcc atctcgtact
    56041 ctttccagcc gatcagcgac tcatcaatca gcaactcttt ggtcggagag agatccagac
    56101 cgcgggcgca aatttcttca aactcttcac ggttataagc gataccgccg ccgctaccgc
    56161 ccatggtaaa ggatgggcga ataatgcacg ggaagcccac gtcagcggca acagccagcg
    56221 cttcttccat cgtgtgtgcg ataccggaac gcgcggtttc cagaccaatt ttcttcatcg
    56281 ctacgtcgaa acgacggcgg tcttctgctt tatcaatcgc atcggcagtg gcaccaatca
    56341 tggtgacacc gaactcttcc aacacgccct gacgttccag ctccagcgcg cagttcagcg
    56401 ccgtctgacc gcccatggtt ggcagcaccg cgtccgggcg ctctttttca ataatcttgc
    56461 gtaccacttc ccagtgaatc ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca
    56521 tgatggtcgc cgggttggag ttcaccagaa tgacgcggta accctcttcg cgcagggctt
    56581 tacatgcttg cgcgccagag tagtcaaact cacacgcctg accgataaca atcgggcccg
    56641 cacccagaat caggatactt tttatatctg tacgttttgg catggctctt ttactcctga
    56701 ttacttagcg gttttacggt actgctcaat taacttgata aagtggtcga acaacggcgc
    56761 ggcgtcgtgt ggaccagggc tggcttcagg gtgaccctgg aagctgaacg ccggcttatc
    56821 gatgcgatga atgccctgta acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt
    56881 tgcaggtaat gttgcttcat ccaccgcaaa accgtggttc tgggcggtga tcatcaccac
    56941 gtttttctcc acatctttaa ccggatggtt gccgccgtgg tgaccaaatt tcattttgac
    57001 agtcttcgca ccgctcgcca gcgccagcag ctgatgaccg agacagatgc cgaataccgg
    57061 aatatcggtt tcgaggaact tctggatggc ggtaatggcg taatcgcacg gggccgggtc
    57121 gccaggaccg ttggagagga agatgccgtc tggattcatt ttcagcacat cttccgcaga
    57181 agtttgcgcc ggaacgatgg tcaggcgaca gcctctatcc accagcatcc gcaggatgtt
    57241 gcgcttggca ccaaaatcat aagccacgac gtggaacggc agctcgtctt cttttttcgc
    57301 ttctggcagg ccgccggtca acgtccagct cccttgtgtc cagctatagg cttctgcggt
    57361 ggtcacttct tttgccagat ccatgccgtt cagacctggg aacgcgcggg ctttttctaa
    57421 cgccagcgcc gcatccgggt tatcgcctgc gataatgcag ccattctgtg cgcctttctc
    57481 gcgcagtaaa cgcgtcagct tacgggtatc gatatcggca atcgccacga tgttatggcg
    57541 cttcaggtaa gaagagaggt cttcggtatt acggaagttg ctggcaatca gcggcaggtc
    57601 gcgaatcacc agaccttgtg catgtacctg agaagattct tcatcggcgt cattagtgcc
    57661 gacattgcca atatggggat aagtaagagt aacgatttga cgagaatagg aaggatcagt
    57721 gaggatttct tgataaccgg tcattgaagt attgaaaacg acttccccaa ccgccgaacc
    57781 tgttgcccct atggcccgac cgtgaaactg ggttccgtct tccagaacca atagcgctga
    57841 cttaatcaaa acaccctcca gagaatactc actcacttta tttgcatatt aattcacagt
    57901 gatgacgtaa atcaatgcaa atctgcttac cgacgaattt ctggcaaacg gcggcattct
    57961 ggagatatgg gcgctaaaag tcaacttaat ggcaggattt tttatattat tttgtggctc
    58021 atgcatgttt tacaggcatt aagcgacaaa aacatcatct aacctgttct ggaaaacgct
    58081 tgcgcagcaa gaagattgca tgaaataaaa acgagcagga aaaagtggac caaatggtca
    58141 aaatttacat taaaacaaca aaaatagaag acgaaacatg taatttttgg cagttataga
    58201 aaatttaaaa gcataacacc aaaaataaaa gggctaaaaa ttaaagccct ttataattaa
    58261 taaattaaat gtgttacttt tgcatcataa taaacatttt gtggttacaa attattgaga
    58321 tcaagcacat ctcgcatatc aaaaagacca ctttccttac cactcaacca caaagccgat
    58381 cttaccgcac cgttagcaaa tgtcatgcgg ctggacgcct tatgggtgat ctccagacgc
    58441 tcgccaatat cggcaaacat cgcggtatgt tcgccaacga tgtcacctgc acgcacggta
    58501 gcaaaaccaa tggtacctgg cacacgttca ccggtatggc cttcacgact gtagaccgcg
    58561 caatctttca gatctttatc aagggcgtgg gcgatcgcct ctcccattgc cagtgcggtg
    58621 cctgacggcg catcaacttt atgtctatga tgtgcttcaa taatttcgat atcggtgtag
    58681 tcacccatca ctttggctgc tttctccagc agcttaagca tgacgttaac gccaacgcta
    58741 aaattggcgg caaagacaat cgcaatatcg gcggcggcgt cacgaattgc ctgtttaccg
    58801 gcttcgtcaa accccgtagt gccgatcacc atccctttgc catgctgccg acaaaaagcg
    58861 agatggttca gcgtaccttc cggacgggta aaatcgataa acacatcaaa atcatctttt
    58921 atcgcatcaa gactgctttg cacggtaacg cctgttttcc ctgcaccagc cagctcaccg
    58981 gcgtcgctgc ccagtaaaga agatccttcg cgctccagcg ccgcgcccag ctgcacgcct
    59041 tctaatgcca gtgccgcctg aatcaactgg cggcccatac gccccccggc tcccgcgatg
    59101 gcaacgcgga tgtttgcatc atgcatagct attctctttt gttaatttgc atagaccgtt
    59161 ttcagagtaa ccagcgcatc gctacgccgc cagccaaaaa cgctgataat tagaaattaa
    59221 tgataaacag ggagggatat cagtgaaagg catgagtcga tgaatgactg catgccgata
    59281 acatgtgaca ggttacggca ccagagccag cacctcagcc acccactgtt ggaagccttt
    59341 tacattcaga tccaatgcca cctgtacatt ggctggtttg cccaggcaac cgtcgatatc
    59401 aaccaccgtc gtgcctgagg taaattcgcc ttgagtttct accgcgacaa aacacggctt
    59461 gagggtgaat aagtccgggc gcaccagcca ggcgatggcg cagagatcgt gcattcgcag
    59521 accgctttgc atactgccgc tacggtagtg gctaaacagg gcgtggagca tttttccggt
    59581 gcgattcaac tccggtaaag tagcgagata gtcaggggtt aatattgcct gattggtgac
    59641 atccagaccg cacatgacaa tttcgatgcc gctacggaag acacaggcag ccgcttctgg
    59701 atcggcagcg atattaaact cggcatttgg tgtacagttg ccgcgaccag cagaaccgcc
    59761 catgatcacc agacgacgaa tatacggttt acattccggg cattgtaaaa gtaacagcgc
    59821 aatattggtt aacgggccga tggccaccag ggtaacgggc tctggtgcac gcatcagggc
    59881 atcccgaatc gccagaaacg ccggtatccc gagtggcttt cggttgtgct caacaaagtc
    59941 gtagccagcc attcccgatt cgccgtgcac agatgctgca tcacgcggtg cgcgtaccag
    60001 cggcacagcg gctccctggg cgagcggaat ctccgcattc cagaaatgca gcagttgcag
    60061 ggcattgcgg gtagttttct caaccgagac attacccgcg acggtggtca tcagttgcag
    60121 gtcgagttcg ggggcaaaaa tcgcggcggc aatggcgacg gcatcgtcaa tgccggggtc
    60181 agtatcgagg aagataggta aacgcatgtt ttcttcataa aaaatgccgg taacaagacc
    60241 ggcattttcg cataacttag gctgctaatg acttaatcga cttcacgaat atcgacacgc
    60301 agctctttcg gcacttcgaa aacaatattt tcttcacggc cttccagcgg aatggcttca
    60361 ccaccaccca actgctgcaa acgtgccacc acattctgca ccagaatatc cggagccgat
    60421 gcgcccgcag tcacgccgac gcatttaacc tctttcaccc actcttcctg gatatctttc
    60481 gcatcgtcaa tcaaaaacgc gtgtttgccc atacgctggg ccagctccgc cagacggttg
    60541 gagttggagg agtttttcga accgaccacc aacacaactt ccgcctgttc tgccagggcg
    60601 cgtaccgctt cctgacggtt agtcgtggcg tagcagatgt catctttgcg cggaccgaca
    60661 attttcggga agcgtttacg cagcgcgtcg atcacatcag acgtgtcatc caccgacagc
    60721 gtggtttggg tcataaagga gagcttctct tcgtttttga ccgtcagttt ccacacatcg
    60781 tctggcgatt cgactagata cattccccct tcggggttgc tgtactgccc catcgtccct
    60841 tccacttccg ggtgcccggc gtggccgatg agaatagatt cttcgccacg gcgactggcg
    60901 cgggcaactt ccatatgcac tttggtcacc agcggacagg tggcatcaaa caccgtcaaa
    60961 tcgcggcttt tcgcttcgtt acgaaccgcc tgagaaacac cgtgtgcgga gaaaatcagg
    61021 atcgcgccgt ccggcacttc gctaatctgc tcaataaaga tagccccacg ctcgcgcaag
    61081 ctatcgacca cgtagcggtt atgcaccact tcgtgacgga catatatcgg tgcgccgtaa
    61141 atggccagcg cgttttcaac aatgctgata gcgcggtcta ccccggcaca aaaaccacgc
    61201 gggttggcca acaggatctg catgttacgc ctccagtgcc ggatcgattt ccagcacttc
    61261 aatatcaaaa tgaacggtct gcccggccag cggatggttg aaatcaacgg taatggagtc
    61321 gccgttaatt tcgcggatca caccaggcat ctcactgcca tccattgcgg taaaaagcat
    61381 gattgcgcca atttctggct cgcctgcatc cataaattca cggcgggaga agtactgaat
    61441 caggtccggt gacggcacgc caaacgccgc atctggctcc agcgagaagg tggttttgtc
    61501 gcccactttc agccccagca ggtgttgctc cagcccttca gaaagagaag catcgcccag
    61561 gcggaacagc gccggtttac cgttgttgcg agtagactcg gcggtggtgc catcgtcgag
    61621 ttttagcgtg aagtgcacca ggacggcgct attgctctgt acagattcag acatgcaggt
    61681 ttgctcttat ttttgaggat ttttgccgga tgcggcctac gatttcgtag caatctgtag
    61741 gccgcatccg gcaggattta ttattgtttt ttcgctttgg aaggtaaaaa cccttccagc
    61801 acgatcagtg ccgcaccgac acaaatggca gtatcggcaa ggttgaaggt ggcgaagtgc
    61861 cagtcgccga cgtagaagtc gatcatatcg acaacgaagc cgtgccacag gcggtcgaac
    61921 aggttgccca gcgcgccgcc aataatcagc gcgtaagcga tattgtttag cttctgcgtg
    61981 gccttcgagc gatacatcat cactgccagg agcacgctaa taccaatcgc aataccggca
    62041 aagaaccaac gctgccagcc gccgctatcg gcaaggaaac taaacgccgc gccatagtta
    62101 cgcgcataat gcagattaag cgacgggaac agcgggaccg tatcccccag agcaaagttc
    62161 tggaggatca ggtatttgct gcccagatcg ataatcagca cgactaccac cagccacagc
    62221 cagcgtagcc ctgttgaaca gatcgattga ctcatcaggc aaacttacgt ttttcaccgt
    62281 caccggcgac gttgctgaca cagcggccgc agatttctgc gtgttccgcc accttgccga
    62341 catcctgggt gtagtgccag cagcgtgggc acttctcacc ttcggcttta ctcaacgcga
    62401 ctttcagccc tttgagtact tcgctctgct gagcatcagc aggtgcgtcg ttatagtctg
    62461 caacggtagc gccggaggtc aacaggacaa atcgtaattc atcgcccagc gcggtcagtt
    62521 tcgccgccag ttccggttct gcatacaagg ttactgccgc ttccagcgag ccacccactt
    62581 tcttgtcggc acgcgcttgc tcaatgactt tgttcacttc gccacgcact ttcaacagct
    62641 cgtcccagaa cgcatcgttc attgcttcac tgtctgccag accaaacagg ccttcgtacc
    62701 actcaccggt gaagacgtat ttttcacgtt cgcccggcag atagccccac acttcatcag
    62761 cggtgaagga gaggattggt gccatccagc gcaccagcgc ttcagcgatg tgatacagcg
    62821 cagtctggca gctacgacgc gccacgctat ccgctttggc ggtgtactga cggtctttga
    62881 tgatgtcgag gtagaaggaa cccatctcaa cggagcagaa gcgcatcaga cgctgtacca
    62941 cttcgtggaa atcgtatgct tcgtacgcct tgaggatgtc ttcctgtgcc gctttcgcac
    63001 aacctacggc ccagcgatcc agtaccacca tctcttccgg tttcaccata tcttttgctg
    63061 gatcaaaacc attcaggttt gccagcagga agcgcgcggt gttacggata cgacgatagc
    63121 tatcggcagc acgtttcagg atctcgtcag aaacggccat ttcaccggtg tagtcggttg
    63181 atgccaccca cagacgcaga atatccgcgc ccagtttgtt catcacatcc tgcggcgaaa
    63241 cggtgttgcc gatggattta gacatcttgc gaccctgacc atccacggta aagccgtggg
    63301 tcagtacctg acgatacggc gctttgcctt tcatcgcggt ggagatcatt agggaagaca
    63361 tgaaccaacc acggtgctgg tcagaacctt ccagatacat gtccgctgcg tgaccggcaa
    63421 attccggacg cacgtcaaca acagaagagt gggtagatcc ggagtcaaac catacgtcga
    63481 gagtatccgg cactttcaca tactggtcag cttcgtcgcc gaggatctct ttcgcatcga
    63541 gatcccacca cgcctggatg ccgtcaactt caacgcgttt agccacttct tccatcagtt
    63601 cgagggtacg cggatgcagc tcttccgtgt ctttgtgcac gaacagtgac atcggtacgc
    63661 cccaggtgcg ctgacgggag atacaccagt caggacggtt agcaaccatc gactcgatac
    63721 gcgcctggcc ccagtccggg agccactgca cgcctttgat ctctttcagt gactgcgcac
    63781 gcagaccttt ctgatccatg ctgacgaacc actgcggtgt cgcgcggaag atgatcggcg
    63841 ttttgtgacg ccagcagcac ggatagctgt gctgcatttt ctcaacgtgc agcagcgcgc
    63901 ctttttcctg cagcagcgca acgacgatgt cgttcgcttt gaagacgttc acgccatcca
    63961 gcgtcggata agtgcccggc agataagtgc cgtccgggcc aaccgggtta gcggtttcca
    64021 ggccgtattt ctgaccgatc acatagtcgt ccgggccgtg gccaggcgcg gtgtgaacgg
    64081 caccggtacc ggcatccagg gtaacgtgat cgccgaggat tgccggaacg tcgaagccca
    64141 taaacggatg ggtaaagcgc agcagctcaa gctctgcacc ttttaccgtg ccgagaatgg
    64201 tgtaatcggt cacgccgata cgctgcatta cgctttcaac cagatctttc gccagaatca
    64261 cggcctgacc gtcgatctgc accagcgcat agtcgaaatc aggtgcaata gagattgcgc
    64321 ggttcgcagg cagagtccac ggcgtagtgg tccagatcac cagcgagatt gggccgttaa
    64381 cgttgcttac gccaaatttc gctttcagcg catcctgatc gaccgcctgg aaagcgacgt
    64441 caatggacgg agaagttttg tcgtaatact caacttccgc ttctgccagt gcagaacggc
    64501 agtctacgca ccagtgcacc ggcttcgcgc ctttgtgcag gtgaccattg ccgatgattt
    64561 tgcccagcgc gcggatgatg ttggcttcag ttttgaagtc catagtcaga tacgggtgcg
    64621 accagtcgcc cagcacgccc agacggataa agtctttgcg ttgaccgtca acctgggtcg
    64681 ccgcgtattc gcggcacttg gcgcggaact cggcggcggt gaatttctca cccggcttac
    64741 cgtattcttg ctctactttc agctcgatcg gcagaccgtg gcagtcccag ccaggcacat
    64801 acggcgagtc ataaccggaa agccctttgg acttcacgat aatgtctttc agaatcttgt
    64861 taaccgagtg accaatatga atgctgccat tcgcataagg agggccatca tgcagaatga
    64921 aggttttttt gcctttttta gccgcacgga tgatgccgta cagatcatca tcagtccaac
    64981 gcgccagcat tcccggttcg cgcttggcga gatcgccacg catcgggaac cctgtttccg
    65041 gcaaattcag ggttgattta tagtcactca tcagattctc ggttccgtgt ttcggtttga
    65101 ttacataaca ggcttaagcc ggttttgtta gcccaaaaaa ttcgcgggcg gttaattcat
    65161 cacgcgcaat ctgcgctttc agttcgtcca gcgacgcaaa tcgctgctca ttgcgtattt
    65221 ttttacgcag cactacttgt atatggcgac cgtaaaggtc cattgcaaca tctaacaaat
    65281 gcacttccag ctgctggcga ataccggcaa ccgttgggcg tgttccgatg tttgccacgc
    65341 cgggtaacgg cttttcaccg aggcccagca cttctaccgc ataaacccct ttcaccgggg
    65401 aaacctgacg gcgtagcggt acattcgccg tcgggaaacc tatagtgcgc cctaattcat
    65461 caccgtggac tacacgcccg gagatggcaa acgggtgccc cagtaaactt tctgccagag
    65521 ccagattgtc atcagccagc gcctgacgca cggcggtgct gctgatacgc acaccacctt
    65581 cgcaaaaagt ttgcgtactg gtgatatcga agccgtattc catgccagct ttctgtaata
    65641 acaagaaatc gccttcacga ccagcgccaa agcggaaatc atcacctacg gcaagaaatt
    65701 ttacgcgcaa atgcttcacc aaaagatcgc tgatgaaatt ttgcgcggtt aacgccgcaa
    65761 aacgcctgtc gaaacgcacg cacagcacgt aatcaacgcc acactctgca aggtaacgca
    65821 gtttttcccg cagccgggtc agtcttgccg gggctttatc ggtagcaaac agttccagtg
    65881 gttgaggttc aaaaagcatc accatcaccg gtaagttgcg cttgcgccct tcttcctgca
    65941 agccctgtaa cagcgcgcga tgaccgcgat gcacgccgtc gaaattacca atagtcagca
    66001 cacacccttc ttgcggggcc tggctgagat tatgtatgcc gcgtatcagc ttcatgtctg
    66061 gctcaaaaca gtgaaaatcg tccgagtata ccttgtacag cggtcaaggt taaccggcga
    66121 ttgagtaccg aaatcagaaa gaagtggctt ttcattgcca tggagcaaat cacgggaaga
    66181 aactgaccgc ctgctgcaat ttttatcgcg gaaaagctgt attcacaccc cgcaagctgg
    66241 tagaatcctg cgccatcact acgtaacgag tgccggcaca ttaacggcgc ttatttgcac
    66301 aaatccattg acaaaagaag gctaaaaggg catattcctc ggcctttgaa ttgtccatat
    66361 agaacacatt tgggagttgg accttggcta atatcaaatc agctaagaag cgcgccattc
    66421 agtctgaaaa ggctcgtaag cacaacgcaa gccgtcgctc tatgatgcgt actttcatca
    66481 agaaagtata cgcagctatc gaagctggcg acaaagctgc tgcacagaaa gcatttaacg
    66541 aaatgcaacc gatcgtggac cgtcaggctg ctaaaggtct gatccacaaa aacaaagctg
    66601 cacgtcataa ggctaacctg actgcacaga tcaacaaact ggcttaatcg ccaatttgct
    66661 gaagctttgt gaaaaagccc gcgcaagcgg gtttttttat gcctgctgct tttgtgatgc
    66721 gtgagagaac acactgccgg ttgccagata gtcaaaagag acatgccgtt gtactatctg
    66781 aactcttcgt ggcttaagaa tcgtagaacc atcggctgat acattctgaa caagaatatc
    66841 tactcaatct ccagactccc ccacaaagaa tatggatatt gtgatacaca ttgaggtatt
    66901 aaacttaaga ccactctatt tttgaattaa attcctatat actttaacca ttcataaata
    66961 tctcctcatt agacctt
//
LOCUS       DAJVQR010000009        66642 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVQR010000009 DAJVQR010000000
VERSION     DAJVQR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OQM52_000616"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OQM52_000616"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1562586.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OQM52_000617"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OQM52_000617"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1562587.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OQM52_000618"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OQM52_000618"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1562588.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OQM52_000619"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OQM52_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1562589.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OQM52_000620"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OQM52_000620"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1562590.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRHHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OQM52_000621"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OQM52_000621"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1562591.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OQM52_000622"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OQM52_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1562592.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OQM52_000623"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OQM52_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1562593.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OQM52_000624"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OQM52_000624"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1562594.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OQM52_000625"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OQM52_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1562595.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OQM52_000626"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OQM52_000626"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1562596.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OQM52_000627"
     CDS             complement(15665..16702)
                     /locus_tag="OQM52_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1562597.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OQM52_000628"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OQM52_000628"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1562598.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OQM52_000629"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OQM52_000629"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1562599.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OQM52_000630"
     CDS             19444..19884
                     /locus_tag="OQM52_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1562600.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OQM52_000631"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OQM52_000631"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1562601.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OQM52_000632"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OQM52_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OQM52_000633"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OQM52_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1562602.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OQM52_000634"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OQM52_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS1562603.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OQM52_000635"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OQM52_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCS1562604.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OQM52_000636"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OQM52_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1562605.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OQM52_000637"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OQM52_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1562606.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OQM52_000638"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OQM52_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1562607.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OQM52_000639"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OQM52_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1562608.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OQM52_000640"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OQM52_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1562609.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OQM52_000641"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OQM52_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1562610.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OQM52_000642"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OQM52_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1562611.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OQM52_000643"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OQM52_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1562612.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OQM52_000644"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OQM52_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1562613.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OQM52_000645"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OQM52_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1562614.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OQM52_000646"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OQM52_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1562615.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="OQM52_000647"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="OQM52_000647"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1562616.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="OQM52_000648"
     CDS             37412..37681
                     /locus_tag="OQM52_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1562617.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="OQM52_000649"
     CDS             complement(37684..38043)
                     /locus_tag="OQM52_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1562618.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="OQM52_000650"
     CDS             38184..38810
                     /locus_tag="OQM52_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1562619.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="OQM52_000651"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="OQM52_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1562620.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OQM52_000652"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OQM52_000652"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1562621.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="OQM52_000653"
     CDS             41650..42315
                     /locus_tag="OQM52_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1562622.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="OQM52_000654"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="OQM52_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1562623.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="OQM52_000655"
     CDS             complement(42949..44166)
                     /locus_tag="OQM52_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1562624.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="OQM52_000656"
     CDS             44300..45349
                     /locus_tag="OQM52_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1562625.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="OQM52_000657"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="OQM52_000657"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1562626.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="OQM52_000658"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="OQM52_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1562627.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="OQM52_000659"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="OQM52_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1562628.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="OQM52_000660"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="OQM52_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1562629.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="OQM52_000661"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="OQM52_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1562630.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="OQM52_000662"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="OQM52_000662"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1562631.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="OQM52_000663"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="OQM52_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1562632.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="OQM52_000664"
     CDS             complement(51544..51903)
                     /locus_tag="OQM52_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1562633.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="OQM52_000665"
     CDS             complement(51900..52175)
                     /locus_tag="OQM52_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCS1562634.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="OQM52_000666"
     CDS             complement(52234..53358)
                     /locus_tag="OQM52_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1562635.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OQM52_000667"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OQM52_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1562636.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="OQM52_000668"
     CDS             complement(56090..57157)
                     /locus_tag="OQM52_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1562637.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="OQM52_000669"
     CDS             complement(57523..59145)
                     /locus_tag="OQM52_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1562638.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="OQM52_000670"
     CDS             complement(59407..60996)
                     /locus_tag="OQM52_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1562639.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="OQM52_000671"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="OQM52_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="OQM52_000672"
     CDS             complement(62499..62975)
                     /locus_tag="OQM52_000672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562640.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="OQM52_000673"
     CDS             complement(62968..63732)
                     /locus_tag="OQM52_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1562641.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="OQM52_000674"
                     /pseudo
     CDS             63978..64739
                     /locus_tag="OQM52_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64846..65346)
                     /locus_tag="OQM52_000675"
     CDS             complement(64846..65346)
                     /locus_tag="OQM52_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562642.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65497..>66642)
                     /locus_tag="OQM52_000676"
     CDS             complement(65497..>66642)
                     /locus_tag="OQM52_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1562643.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16190 a        17888 c        16345 g        16219 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc tccaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggtgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccaag tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctgacgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtaca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgtc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctaaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc acgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatca cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttc cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcgctt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggcctgaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcctggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatt
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaatatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg atgccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaacatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggaaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcataa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgatgcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaatcccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gccttccgca ctcaagccag aaacataagc
    65641 aataccatct tcacctacaa tgccggccat attttcagtg tctttgctat aactaatcac
    65701 agcgccgaag gggatgggtt taccctcttt ctgttttaag gttactaatc ctttatcccc
    65761 gctcttcact gccaatgtcg caggcacaac ggctccagct gtcggtacaa ccaattgact
    65821 agtttgtggt aagtcgtaat tcttacctat cgttttttca tccagaataa catcgtctct
    65881 tcggtatggc tgcactggca gtattgcata cccacgaaaa tcagtactca gtcccggcca
    65941 gccattaacg ttaacccccg ttgctccggg tgcctctata agggctaatg tcccatccac
    66001 attttgacca aatgtaagtc catcccttgt aataagtatc ccaccgctta aatttgcacc
    66061 tagctgtttc atgttttcgc tggagctgta atggctacta aactgaccat atgatccatt
    66121 tttgccaact tgcacaatgt tcatcgtgct cttcggttgt gactgatgag cgttagttat
    66181 atttgtcgct acatcccacc atacaccacc actattctga cctgataatg aaacctgata
    66241 gttattaccc tgctctcccg aacgactcat ctgtgaagac gcataaattt gattttcgtc
    66301 acccagcgat aatggcactg aaatacccac gcttaatttc cgatctactt tccatttatg
    66361 cgatgtcagc aagtcgtttt ttgcccagtt aatggagaat gttatgcctc tccataccgt
    66421 actataactc aggctggtag accgcgtgtt tcctgtgccc cgggaagaat tatacctcgt
    66481 ggtattgaag ttcaaattcc cgatattacc aaggggctgg gataaggata tcgtagagcc
    66541 atcgtgacta ttcttatctc tttttctgtt ccgttcatcc tgtgcactta ctgcttcagt
    66601 cagtgtggca taacctgatg cataacggtt cgtggttata ga
//
LOCUS       DAJVQR010000010        65689 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVQR010000010 DAJVQR010000000
VERSION     DAJVQR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OQM52_000677"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OQM52_000677"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1562644.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQM52_000678"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQM52_000678"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1562645.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQM52_000679"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQM52_000679"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1562646.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQM52_000680"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQM52_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1562647.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQM52_000681"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQM52_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1562648.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQM52_000682"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQM52_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1562649.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OQM52_000683"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OQM52_000683"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1562650.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10140
                     /gene="mrcB"
                     /locus_tag="OQM52_000684"
     CDS             7606..10140
                     /gene="mrcB"
                     /locus_tag="OQM52_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1562651.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10360..12603
                     /gene="fhuA"
                     /locus_tag="OQM52_000685"
     CDS             10360..12603
                     /gene="fhuA"
                     /locus_tag="OQM52_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1562652.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12654..13451
                     /gene="fhuC"
                     /locus_tag="OQM52_000686"
     CDS             12654..13451
                     /gene="fhuC"
                     /locus_tag="OQM52_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1562653.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13451..14341
                     /gene="fhuD"
                     /locus_tag="OQM52_000687"
     CDS             13451..14341
                     /gene="fhuD"
                     /locus_tag="OQM52_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1562654.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14338..16320
                     /gene="fhuB"
                     /locus_tag="OQM52_000688"
     CDS             14338..16320
                     /gene="fhuB"
                     /locus_tag="OQM52_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1562655.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="OQM52_000689"
     CDS             complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="OQM52_000689"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1562656.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17860..19281
                     /gene="clcA"
                     /locus_tag="OQM52_000690"
     CDS             17860..19281
                     /gene="clcA"
                     /locus_tag="OQM52_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1562657.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19305..19370
                     /locus_tag="OQM52_000691"
     CDS             19305..19370
                     /locus_tag="OQM52_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562658.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19363..19707
                     /gene="erpA"
                     /locus_tag="OQM52_000692"
     CDS             19363..19707
                     /gene="erpA"
                     /locus_tag="OQM52_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1562659.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19754..20377)
                     /locus_tag="OQM52_000693"
     CDS             complement(19754..20377)
                     /locus_tag="OQM52_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1562660.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="OQM52_000694"
     CDS             complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="OQM52_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS1562661.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="OQM52_000695"
     CDS             complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="OQM52_000695"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1562662.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21990..23507
                     /gene="dgt"
                     /locus_tag="OQM52_000696"
     CDS             21990..23507
                     /gene="dgt"
                     /locus_tag="OQM52_000696"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1562663.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23637..25061
                     /gene="degP"
                     /locus_tag="OQM52_000697"
     CDS             23637..25061
                     /gene="degP"
                     /locus_tag="OQM52_000697"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1562664.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25216..26372
                     /gene="cdaR"
                     /locus_tag="OQM52_000698"
                     /pseudo
     CDS             25216..26372
                     /gene="cdaR"
                     /locus_tag="OQM52_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26461..26847)
                     /locus_tag="OQM52_000699"
     CDS             complement(26461..26847)
                     /locus_tag="OQM52_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1562665.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="OQM52_000700"
     CDS             complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="OQM52_000700"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1562666.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="OQM52_000701"
     CDS             complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="OQM52_000701"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1562667.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30750..31544)
                     /gene="map"
                     /locus_tag="OQM52_000702"
     CDS             complement(30750..31544)
                     /gene="map"
                     /locus_tag="OQM52_000702"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1562668.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31912..32637
                     /gene="rpsB"
                     /locus_tag="OQM52_000703"
     CDS             31912..32637
                     /gene="rpsB"
                     /locus_tag="OQM52_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1562669.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32895..33746
                     /gene="tsf"
                     /locus_tag="OQM52_000704"
     CDS             32895..33746
                     /gene="tsf"
                     /locus_tag="OQM52_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1562670.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33893..34618
                     /gene="pyrH"
                     /locus_tag="OQM52_000705"
     CDS             33893..34618
                     /gene="pyrH"
                     /locus_tag="OQM52_000705"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1562671.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34910..35467
                     /gene="frr"
                     /locus_tag="OQM52_000706"
     CDS             34910..35467
                     /gene="frr"
                     /locus_tag="OQM52_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1562672.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35559..36755
                     /gene="ispC"
                     /locus_tag="OQM52_000707"
     CDS             35559..36755
                     /gene="ispC"
                     /locus_tag="OQM52_000707"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1562673.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36944..37702
                     /gene="ispU"
                     /locus_tag="OQM52_000708"
                     /gene_synonym="uppS"
     CDS             36944..37702
                     /gene="ispU"
                     /locus_tag="OQM52_000708"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1562674.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37715..38572
                     /gene="cdsA"
                     /locus_tag="OQM52_000709"
     CDS             37715..38572
                     /gene="cdsA"
                     /locus_tag="OQM52_000709"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1562675.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38584..39936
                     /gene="rseP"
                     /locus_tag="OQM52_000710"
     CDS             38584..39936
                     /gene="rseP"
                     /locus_tag="OQM52_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1562676.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39966..42398
                     /gene="bamA"
                     /locus_tag="OQM52_000711"
     CDS             39966..42398
                     /gene="bamA"
                     /locus_tag="OQM52_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1562677.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42520..43005
                     /gene="skp"
                     /locus_tag="OQM52_000712"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42520..43005
                     /gene="skp"
                     /locus_tag="OQM52_000712"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1562678.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43009..44034
                     /gene="lpxD"
                     /locus_tag="OQM52_000713"
     CDS             43009..44034
                     /gene="lpxD"
                     /locus_tag="OQM52_000713"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1562679.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44139..44594
                     /gene="fabZ"
                     /locus_tag="OQM52_000714"
     CDS             44139..44594
                     /gene="fabZ"
                     /locus_tag="OQM52_000714"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1562680.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44598..45386
                     /gene="lpxA"
                     /locus_tag="OQM52_000715"
     CDS             44598..45386
                     /gene="lpxA"
                     /locus_tag="OQM52_000715"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1562681.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45386..46534
                     /gene="lpxB"
                     /locus_tag="OQM52_000716"
     CDS             45386..46534
                     /gene="lpxB"
                     /locus_tag="OQM52_000716"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1562682.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46531..47127
                     /gene="rnhB"
                     /locus_tag="OQM52_000717"
     CDS             46531..47127
                     /gene="rnhB"
                     /locus_tag="OQM52_000717"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1562683.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47164..50646
                     /gene="dnaE"
                     /locus_tag="OQM52_000718"
     CDS             47164..50646
                     /gene="dnaE"
                     /locus_tag="OQM52_000718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1562684.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50659..51618
                     /gene="accA"
                     /locus_tag="OQM52_000719"
     CDS             50659..51618
                     /gene="accA"
                     /locus_tag="OQM52_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1562685.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51717..53858
                     /gene="ldcC"
                     /locus_tag="OQM52_000720"
     CDS             51717..53858
                     /gene="ldcC"
                     /locus_tag="OQM52_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1562686.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53915..54304
                     /locus_tag="OQM52_000721"
     CDS             53915..54304
                     /locus_tag="OQM52_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1562687.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54369..55667
                     /gene="tilS"
                     /locus_tag="OQM52_000722"
     CDS             54369..55667
                     /gene="tilS"
                     /locus_tag="OQM52_000722"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1562688.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55716..55976)
                     /gene="rof"
                     /locus_tag="OQM52_000723"
     CDS             complement(55716..55976)
                     /gene="rof"
                     /locus_tag="OQM52_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1562689.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55963..56163)
                     /locus_tag="OQM52_000724"
     CDS             complement(55963..56163)
                     /locus_tag="OQM52_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1562690.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56329..56874
                     /locus_tag="OQM52_000725"
     CDS             56329..56874
                     /locus_tag="OQM52_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1562691.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56871..57293
                     /gene="arfB"
                     /locus_tag="OQM52_000726"
     CDS             56871..57293
                     /gene="arfB"
                     /locus_tag="OQM52_000726"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1562692.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57307..58017
                     /gene="nlpE"
                     /locus_tag="OQM52_000727"
                     /gene_synonym="cutF"
     CDS             57307..58017
                     /gene="nlpE"
                     /locus_tag="OQM52_000727"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1562693.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58172..58996)
                     /locus_tag="OQM52_000728"
     CDS             complement(58172..58996)
                     /locus_tag="OQM52_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS1562694.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59049..60767)
                     /gene="proS"
                     /locus_tag="OQM52_000729"
     CDS             complement(59049..60767)
                     /gene="proS"
                     /locus_tag="OQM52_000729"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1562695.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="OQM52_000730"
     CDS             complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="OQM52_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1562696.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="OQM52_000731"
     CDS             complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="OQM52_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1562697.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="OQM52_000732"
     CDS             complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="OQM52_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1562698.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62959..63612)
                     /gene="metI"
                     /locus_tag="OQM52_000733"
     CDS             complement(62959..63612)
                     /gene="metI"
                     /locus_tag="OQM52_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1562699.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63605..64636)
                     /gene="metN"
                     /locus_tag="OQM52_000734"
     CDS             complement(63605..64636)
                     /gene="metN"
                     /locus_tag="OQM52_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1562700.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64824..65399
                     /gene="gmhB"
                     /locus_tag="OQM52_000735"
     CDS             64824..65399
                     /gene="gmhB"
                     /locus_tag="OQM52_000735"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1562701.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15533 a        16275 c        18097 g        15784 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccacca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gggatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaaaataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcaggt gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggctgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgtgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg aagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acagcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggttgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgagcg caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca
     7921 gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg
     7981 aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc
     8341 tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg
     8461 cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgtggt
     8821 ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg
     9421 gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggta aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa
    10081 gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa
    10141 catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa
    10201 gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc
    10261 gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa taatcattct
    10321 cgtttacgtt attattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct
    10381 cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg
    10441 tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca
    10501 cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg gcagtcagct
    10561 accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc
    10621 gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg
    10681 ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg accacctgat cattcgcggt
    10741 tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac
    10801 ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc
    10861 ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag
    10921 cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc
    10981 cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg
    11041 accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct
    11101 attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac
    11161 ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag
    11221 ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc
    11281 tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt
    11341 actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt
    11401 tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca
    11461 gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac
    11521 ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg
    11581 ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac
    11641 gactctgtgc cactgttcaa tctgtacaat ccggtgaatc ccgatttcga cttcaatgcc
    11701 aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt
    11761 tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac
    11821 tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg tgatgacaaa
    11881 cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc
    11941 agctatagcg aatcgtttga accttcttcg caagttggga aggatggtaa tattttcgca
    12001 ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt
    12061 gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag
    12121 ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg
    12181 aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa
    12241 tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct
    12301 tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc
    12361 ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt
    12421 tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc
    12481 aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac
    12541 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc
    12601 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12661 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12721 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12781 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12841 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12901 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12961 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    13021 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13081 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13141 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc agcgctggat atcgcccacc
    13201 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13261 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13321 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13381 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13441 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13501 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13561 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13621 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13681 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13741 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13801 gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc
    13861 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13921 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13981 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    14041 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14101 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14161 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14221 catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc
    14281 aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg
    14341 agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14401 gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc
    14461 tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14521 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14581 gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg
    14641 gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg
    14701 ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg
    14761 tcgccggtaa cgctgatttt cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat
    14821 cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga
    14881 acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt
    14941 gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg
    15001 gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt
    15061 gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg
    15121 ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg
    15181 ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15241 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15301 ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg
    15361 actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct
    15421 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15481 ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15541 gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc
    15601 ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat gctgtttctg
    15661 gtaccgggta atgcctttgg ctggttgtta cccgcaggca gtctcggggc ggcggtgacg
    15721 ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg
    15781 gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca ggcaagtggt
    15841 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
    15901 gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc
    15961 cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg
    16021 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16081 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16141 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
    16201 gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16261 ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16321 tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg
    16381 cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcctgctt
    16441 caaacgctga cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa
    16501 aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa
    16561 agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc gcttcttctg
    16621 ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc
    16681 ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg
    16741 aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac
    16801 gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat
    16861 ctggcactac ggcgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca
    16921 cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga
    16981 actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg
    17041 caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac
    17101 aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg
    17161 tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt
    17221 caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg
    17281 tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca
    17341 ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg
    17401 cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc
    17461 aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg
    17521 ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca
    17581 caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg
    17641 gttcctgatt cgtagaaaaa gtgaatggct gctattccat gttattcata acaagttaaa
    17701 tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag
    17761 ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat
    17821 gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct
    17881 ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag
    17941 cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg
    18001 ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg
    18061 ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg
    18121 gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg
    18181 gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta
    18241 ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggg gctagggcgc
    18301 gaaggaccaa ccgtgcagat cggcggcaat atcagtcgca tggtgcttga tattttccgc
    18361 ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct
    18421 gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag
    18481 tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt
    18541 atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg
    18601 ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct
    18661 atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat
    18721 attaccaaat gggtactgat ggggggggcg attggcggtc tgtgtggatt gctggggttt
    18781 gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat
    18841 ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc
    18901 ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg
    18961 ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg
    19021 acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg
    19081 gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt
    19141 accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg
    19201 attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca
    19261 tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg attattattg
    19321 ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca
    19381 ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa
    19441 gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag
    19501 tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc
    19561 gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac
    19621 accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc
    19681 ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa actatccggc
    19741 acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc
    19801 aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt
    19861 ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg
    19921 cagttgcgta gatttctgta cgtaaaatca tggggatttc gcggaccagt acatcacgaa
    19981 tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt
    20041 ccgcattaaa ggctttattc acgctaatgc cgacaaacac cgccagacca acggcgtcca
    20101 gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca
    20161 tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt
    20221 gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca
    20281 gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccgtag atggcaaata
    20341 ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct
    20401 gaaaaggtca ggcactaatc tacctgtgaa agtgcattac agagctgttg tgctgcgagg
    20461 ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg
    20521 agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca
    20581 atagcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg
    20641 ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct
    20701 tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta
    20761 tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg
    20821 gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca
    20881 atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc
    20941 cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt
    21001 tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag
    21061 tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg
    21121 gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac
    21181 agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc
    21241 aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga
    21301 ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg
    21361 gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg
    21421 gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc
    21481 acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc
    21541 gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt
    21601 gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt
    21661 tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa
    21721 cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac
    21781 ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg
    21841 gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat
    21901 tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg
    21961 ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa
    22021 actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga
    22081 tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa
    22141 agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga
    22201 tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc
    22261 ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg
    22321 agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag
    22381 cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga
    22441 ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg
    22501 agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc
    22561 gtctggtgca tacattgatg cggatgaatc tcacttgggc acaggttggc ggtattttaa
    22621 aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga
    22681 aaaagccggg ttattatctt tctgaagaag cctatattgc ccgcttgcgt aaagaactta
    22741 atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct
    22801 cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc
    22861 tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg
    22921 tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc
    22981 agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac
    23041 gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag
    23101 atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt
    23161 ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat
    23221 tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa
    23281 aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc
    23341 atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc
    23401 tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc
    23461 tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca gtcttttgta
    23521 aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa
    23581 aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga
    23641 aaaaaaacac attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc
    23701 tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg
    23761 caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa
    23821 ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt
    23881 tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta
    23941 atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg
    24001 ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc
    24061 agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata
    24121 tcgcgctgat ccaaatccag aacccgaaaa aactgaccgc aattaagatg gcggattctg
    24181 atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga
    24241 cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg
    24301 aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta
    24361 acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca
    24421 tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg
    24481 aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac
    24541 tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta
    24601 attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta
    24661 agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca
    24721 aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc
    24781 agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga
    24841 tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg
    24901 ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga
    24961 aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca
    25021 ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca accccttcct
    25081 gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc
    25141 atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg
    25201 cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg
    25261 gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt
    25321 atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt
    25381 tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg
    25441 caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca
    25501 ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg
    25561 ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg
    25621 gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc
    25681 tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc
    25741 ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg
    25801 agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt
    25861 tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta
    25921 cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg
    25981 gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac
    26041 agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg
    26101 acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag
    26161 cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc
    26221 aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta
    26281 atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt
    26341 atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga ggcggcgctg
    26401 gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt caattcggga
    26461 ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac
    26521 tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc
    26581 acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg
    26641 gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata
    26701 tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag
    26761 aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat
    26821 acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc
    26881 ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct
    26941 tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc
    27001 catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag
    27061 atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag
    27121 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27181 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27241 agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg
    27301 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27361 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27421 cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27481 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27541 aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccgatgt
    27601 taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg
    27661 ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt
    27721 tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27781 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27841 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27901 cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27961 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    28021 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    28081 tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    28141 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28201 gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat
    28261 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28321 gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa
    28381 taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28441 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28501 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28561 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28621 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28681 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28741 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28801 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28861 tccagctatt ccacagtgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28921 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28981 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg
    29041 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    29101 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    29161 cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc
    29221 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29281 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29341 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagtccg cgccgcaccg
    29401 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg
    29461 gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag taaatgccgg
    29521 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29581 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29641 gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29701 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29761 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29821 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29881 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29941 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    30001 gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    30061 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg
    30121 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30181 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30241 acatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30301 gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30361 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa
    30421 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30481 ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30541 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt
    30601 catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtggag agagcggtgt
    30661 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30721 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30781 gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc
    30841 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30901 cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc
    30961 agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    31021 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    31081 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc
    31141 aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg
    31201 acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg
    31261 ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact
    31321 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31381 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31441 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31501 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31561 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31621 tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc
    31681 gacgtctgat gctgtacaca gcgccaacaa ttattggtgt ccacgacgta tttgtggtat
    31741 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31801 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31861 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31921 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31981 aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt
    32041 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc
    32101 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    32161 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32221 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32281 ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32341 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32401 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta
    32461 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32521 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32581 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32641 ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32701 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgcggcgc
    32761 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32821 catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct
    32881 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32941 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    33001 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    33061 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    33121 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33181 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33241 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    33301 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33361 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33421 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33481 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33541 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33601 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33661 tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33721 ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt
    33781 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33841 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33901 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33961 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    34021 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    34081 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    34141 gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34201 tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    34261 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34321 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34381 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat
    34441 gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34501 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc
    34561 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34621 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt
    34681 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta
    34741 caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta
    34801 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34861 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34921 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34981 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    35041 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    35101 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    35161 ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35221 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35281 gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35341 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35401 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35461 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35521 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35581 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35641 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    35701 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35761 tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35821 tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctgggctgt
    35881 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35941 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    36001 cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc
    36061 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    36121 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcttgcc
    36181 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36241 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36301 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36361 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36421 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36481 ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36541 gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36601 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36661 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgagtgaag
    36721 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36781 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    36841 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36901 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36961 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    37021 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    37081 aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct
    37141 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37201 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37261 gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37321 tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg
    37381 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37441 gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc
    37501 ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg
    37561 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37621 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37681 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37741 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37801 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt
    37861 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37921 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37981 ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    38041 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    38101 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    38161 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38221 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38281 ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    38341 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38401 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38461 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38521 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38581 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38641 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38701 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38761 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38821 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg
    38881 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38941 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    39001 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    39061 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    39121 attacagtag cgccatttga cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    39181 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39241 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39301 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39361 tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39421 gggagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    39481 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39541 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39601 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39661 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39721 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39781 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39841 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39901 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39961 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    40021 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    40081 ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata
    40141 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40201 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40261 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    40321 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40381 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40441 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    40501 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40561 tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac
    40621 tggcgggcga tcttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca
    40681 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40741 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40801 ggcactccgc tgaaattgag caactgacta agatcgagcc gggcgagctg tataacggca
    40861 ctaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct
    40921 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40981 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    41041 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga
    41101 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    41161 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga
    41221 aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga
    41281 gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca
    41341 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41401 ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41461 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41521 ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41581 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    41641 ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41701 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    41761 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41821 tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg
    41881 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41941 ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg
    42001 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    42061 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    42121 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct
    42181 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42241 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42301 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42361 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42421 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42481 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42541 gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42601 aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42661 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42721 atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg
    42781 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42841 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42901 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42961 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    43021 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    43081 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43141 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43201 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43261 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43321 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    43381 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43441 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43501 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43561 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43621 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43681 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43741 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43801 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43861 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43921 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43981 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    44041 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    44101 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44161 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44221 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44281 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44341 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44401 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44461 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44521 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44581 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44641 aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44701 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44761 aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt
    44821 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44881 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44941 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    45001 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    45061 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45121 ccggtgtggc gcaggacgtc cctccttatg ttattgcgca gggtaaccac gcaacgccgt
    45181 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45241 tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45301 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45361 gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt
    45421 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45481 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45541 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45601 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45661 tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa
    45721 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45781 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45841 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45901 gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca
    45961 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    46021 cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct
    46081 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    46141 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46201 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46261 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46321 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46381 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46441 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46501 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46561 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46621 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46681 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46741 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46801 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46861 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca
    46921 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46981 gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat
    47041 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    47101 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47161 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47221 ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47281 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47341 gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat
    47401 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg
    47461 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47521 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47581 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47641 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47701 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47761 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47821 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47881 atgcgtagcg aagaggagat gtgtgagctg tttgccgaca tcccagaagc ccttgccaac
    47941 accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg
    48001 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc
    48061 ctggaagagc gtctggcatt tttattccct gacgaggaag aacgtcttaa acgccgcccg
    48121 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48181 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48241 ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc
    48301 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48361 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg
    48421 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48481 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc
    48541 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48601 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48661 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48721 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48781 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48841 cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48901 ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48961 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    49021 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    49081 cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa
    49141 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca
    49201 acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt
    49261 tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag
    49321 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49381 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49441 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg
    49501 gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49561 ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt
    49621 ctttaccatt tccacgtcaa cgacgatggc gaaatcgtgt atggtattgg cgccatcaaa
    49681 ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc
    49741 cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg
    49801 gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg
    49861 aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49921 caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctacgcc
    49981 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    50041 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    50101 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc
    50161 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50221 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50281 caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50341 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50401 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50461 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50521 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50581 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50641 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50701 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50761 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc
    50821 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca gcgtccttat
    50881 accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgtgcg
    50941 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg
    51001 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    51061 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    51121 atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag
    51181 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct tggcgtaccg
    51241 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51301 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51361 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51421 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51481 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51541 gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51601 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51661 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51721 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51781 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51841 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt
    51901 acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct
    51961 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt
    52021 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacacca
    52081 acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc tacgtcaaag
    52141 agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat caaaaaagcc
    52201 cggttggctg tctgttttat gattttttcg gcgggaatac ccttaaggct gatgtctcta
    52261 tttcggtcac cgagcttggt tcgttgcttg accacaccgg gccacacctg gaagcggaag
    52321 agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac ggaatatcga
    52381 cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc
    52441 gcaactgcca taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc
    52501 tgaaaccgac gcgtaatgcg ttggggattc tgggggggat cccgcgccgt gaatttactc
    52561 gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg gttcatgcgg
    52621 tgatcaccaa ctccacctat gatggtttgc tctacaacac cgactggatc aaacagacgc
    52681 tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtatactcat tttcatccaa
    52741 tctaccaggg taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt ttcttcgaaa
    52801 cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcactgatc cacattaaag
    52861 gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca
    52921 gttatcccat tgttgcttcg gttaagacgg cggcggcgat gctgcgtggt aatccgggca
    52981 aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggc
    53041 tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag
    53101 ccgaatgcag gcccgttgcg cctggcgaac aatggcacgg ctttaacgat gcggatgccg
    53161 atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg
    53221 gcaatatgag tgaggagggg atcccggcgg cgctggtagc aaaattcctc gacgaacgtg
    53281 ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt attggcatcg
    53341 ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaagcgc tcttacgatc
    53401 tcaacctgcg gatcaaaaat atgctgcccg atctctatgc tgaagatccc gatttctacc
    53461 gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc
    53521 tttccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg acgccacatc
    53581 aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg
    53641 gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgccag
    53701 gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg
    53761 tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg
    53821 gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc
    53881 gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca
    53941 tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg
    54001 tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc
    54061 gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg agcgccccag
    54121 ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc
    54181 agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac
    54241 gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca
    54301 gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc
    54361 gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg
    54421 cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg
    54481 aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt gccaatgccg
    54541 atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg
    54601 aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg caggcacgtt
    54661 atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg caacatctcg
    54721 acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt
    54781 cggctatggc ggaagtttcg gagtttgctg gaacacggct tatccgcccg ttgcttgccc
    54841 gcacgcgggg ggaactggag cagtgggcat tggcgcatgg tttacgctgg attgaagacg
    54901 aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat
    54961 tgcagcagcg ttggccgcat tttgcggaag caacggcccg cagcgccaca ctttgtgctg
    55021 aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc caaacgtcgc
    55081 aggggacgct gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc gcggcgatta
    55141 ttcgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgca
    55201 tctggcagga agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt ttgggcgcct
    55261 ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa atccgtcacc gggcaaagcg
    55321 aaaccattgt gctgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa
    55381 ccgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg
    55441 tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg caacggcgga cgtaagctga
    55501 agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt
    55561 tttatggaga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg
    55621 aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat
    55681 aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac caccaccgta
    55741 ccgatttcag ggtggctaaa gctggtaatt ttatccagac gcagctcgcg ggtttcgcca
    55801 gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcact ggcttttgcc
    55861 tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc
    55921 tcaagattat cgtaatcatc acaattaatt ggttgatacg tatcattcat tgacataatc
    55981 gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc
    56041 gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta
    56101 tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc
    56161 cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga
    56221 taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac
    56281 tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa
    56341 gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat
    56401 gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg
    56461 ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat
    56521 gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg
    56581 gggctgccgg atgagcgacg aattaagaaa gcctgcaccc aggccgcaga agtggcgctg
    56641 tttacctata atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg
    56701 cagtttgcca atctttccgt ctggtatctg gacgatgaac aactggcgaa agtaagcgcc
    56761 tttgccgatc gtaccatgac gctgcaggca acgattcagg atggtgttat ctggttatcg
    56821 gatgataaga ataatctgga agttaactta accgcctggc aacaaccttc atgattgtga
    56881 tttcccgaca tgttgctatt cccgatggtg agcttgagat caccgccatt cgtgcgcagg
    56941 gcgctggcgg gcagcatgtt aataagacct caacggctat tcatctgcgt tttgacattc
    57001 gggcgtccag cctgccagag tattacaaag agcgtctgct cgccgccagc catcatttga
    57061 tcagcagtga tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc
    57121 gcgaagccgc tctggcccgg ctggtggcag tgattaaaga tttaacaaaa gaacaaaaag
    57181 cccgacgacc cacgcggccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac
    57241 aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa taaaaagaag
    57301 gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg
    57361 atgggatgta ataatcgggc cgaagtcgat acgctttctc cggcgcaggc tgccgaactg
    57421 aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg ccgattgcga aggaatcgaa
    57481 atctctctgt tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct
    57541 cgtgaagaac cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg
    57601 gtattaaccg acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag
    57661 atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca actatacgct ggaaccggca
    57721 caatccagtt tacctatgac gccgatgacc ctgcgaggca tgtattttta tatggctgat
    57781 gcggcgacct tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag
    57841 ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt actgtcagta
    57901 gaaggtcact ttacgcttga ggctaatccg gatactggtg cgccaaccaa agtattagcg
    57961 cccgatacgg cgggtaaatt ttacccgaat aaggattgca gtagtttggg gcagtaaacc
    58021 tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct gcgcttatca ggcctacagc
    58081 atataatgca acattttgaa ttagcacgat tttgtaggcc ggataaggcg tttacgccgc
    58141 atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg
    58201 ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58261 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58321 gcgccagtgt aagtggatgc ccggatttgg caaatgcatc agtgacaaat tccgaacaaa
    58381 accacgactt tttgtctcct tcgcacacac tgcttaactg cgctttcgcc aggtcgctga
    58441 cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
    58501 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
    58561 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
    58621 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
    58681 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
    58741 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
    58801 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
    58861 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
    58921 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg cggcagtacg
    58981 cctttggttt atccatttta tacaatccat gtaaaaaagg gccctgaaat tcaggaccct
    59041 ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt
    59101 aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg
    59161 tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc
    59221 ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac
    59281 agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc
    59341 acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta gttctgctca
    59401 atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg
    59461 ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc
    59521 tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg
    59581 cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga
    59641 tcccagttga taccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc
    59701 atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa
    59761 cccggaccgg ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt
    59821 gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc
    59881 agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt
    59941 ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta
    60001 tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct
    60061 gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg
    60121 ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggcc
    60181 tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt
    60241 gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc
    60301 aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc
    60361 tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg
    60421 tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca
    60481 aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcggct
    60541 ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg
    60601 atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag
    60661 gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg
    60721 gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc
    60781 cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga tttagtttac
    60841 cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc agcgcaaaga
    60901 cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc cagacggcat
    60961 aggttttgcc cgtttcctca cctttacgat aggccgggcg cgggtcctgc gccagtactt
    61021 cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc tgcttttcga
    61081 cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca tagctggcac
    61141 tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact accggcgtac
    61201 catcgaccag atccagactg ccgagcttca gaatcacgct gcctttatgg caaacaacct
    61261 ctttcagctc taccagcgac atgccaattg ggttagggcg gaaagtagag cgtgttgcga
    61321 aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga cgccagccgc
    61381 cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaatgct tccaggccgc
    61441 gaacggcgtc ggcctggttg taggaagcta tgagatgcag ttcgccgttg gcgcttttta
    61501 ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga ataacgccta
    61561 tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc cgatacatac
    61621 agcctgacga tagcagcccg gcgtaccgct ggtgacttcg cagctatgca gtaatacagc
    61681 attggctttc attttagagg cattgatttg catctgctta cgtgcggttg gaatgctcgg
    61741 cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga gatcgcggaa
    61801 cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg cgcgcggcgc
    61861 tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg gttcgacagg
    61921 ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga tcggtaaagc
    61981 acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg agcttgtaaa
    62041 aatgacaaga cgggcaagcg cccgtcctga atgatattac aaattgtgga aacagcctaa
    62101 aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt cgtaaacttc
    62161 atcagactga taagcctgga cgaatttctt cacgttctcg gcgtctttgt tatcttcacg
    62221 cgttacgatc aggtttacgt atggggagtc tttatcttcg acaaagatac cgtctttcgc
    62281 cggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag cgatttgcgc
    62341 gtcgtccaga gaacgcggca gttgcggtgc ttccagttca acaattttca gattttttgg
    62401 gttctcaaca acatcaagaa cggtcggcag caggccaacg ccatctttca gtttgatcaa
    62461 gcccactttt tgcagcagca gcagtgaacg accaagatta gttggatcgt ttggcacggc
    62521 aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt aaccagcaat
    62581 cggataaaca aaagtgttgc ctactgcgac cagtttgtag ccacgatctt tcagttgctg
    62641 atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc tcaatgcttc
    62701 gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat atttgtcttt
    62761 cgcaactttc tgcgcgactt ctgctacctg ctgttcggca ccaacaatca cgccgacttt
    62821 aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg atccaatcag
    62881 ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct tcttcaatta
    62941 tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt cgcctgcaaa
    63001 ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca ccgtcgcgtt
    63061 gtagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac cgactgcacc
    63121 acccatcgcg gaataaccga ccagggtaat cagggtgata gttgccgcat tcaccagacc
    63181 cggcaacgct tccggtaaca gcactttaca gacgatctgc attggcgtgg cacccattgc
    63241 gcgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga ccatacgggc
    63301 aataaacggt gctgcaccaa cggttaacgg aacaattgct gcctgcaaac caatcgatgt
    63361 accgacaata acgcgggtaa acggaatcat ccataccagc aagataatga acgggatgga
    63421 acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag caataatttg
    63481 ccccggacgc gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa acccaaaaaa
    63541 tccggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca gccacatcat
    63601 cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc caggcaatgg
    63661 cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg aatttaacgc
    63721 caccggcgta gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa cgacgcgcgg
    63781 tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc agcatcggca
    63841 cgcagtcagt aaatggctcc gtttgcagac gttcctggta atcttccggg atatccagat
    63901 gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag aacacttcac
    63961 ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac cgccacgcaa tcacaaatac
    64021 gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga cggcggttaa
    64081 tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg ctggtggctt
    64141 catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc acacgttgtt
    64201 tctgcccacc ggaaagattc gacgggtagc tatcatgctt atcgccaaga ccaaccaatg
    64261 acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc agctccagcg
    64321 gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc tggaaaatca
    64381 taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc aattcctggc
    64441 catcgaccag cacgctaccc tcggttgggc gctccagcaa gtttacacaa cgtataagcg
    64501 tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgtccagct ggcacatgca
    64561 ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg gtggaacact ttggtgatat
    64621 tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtag catctcgaat
    64681 gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac gtctaaatca
    64741 atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact agcgtcccat
    64801 gccttattaa ggagctataa aaggtggcga agagcgtacc cgcaattttt cttgaccgtg
    64861 atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt gaatttatcg
    64921 acggtgttat tgacgccatg cgcgagctaa aaaaaatggg ctttgcgctg gtggtagtaa
    64981 ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc acagtttgaa acgctgaccg
    65041 agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc tattattgcc
    65101 cgcatcatcc gcaggggagt gttgaagagt ttcgccaggt ctgcgattgc cgcaaaccac
    65161 atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc gcttcttata
    65221 tggtgggcga taaattagaa gatatgcagg cagcggttgc ggcgaacgtg ggaacaaaag
    65281 tgctggtgcg tacgggtaaa cctattacac ctgaagcaga aaacgcggca gattgggtgt
    65341 taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg gcgcaatgat
    65401 taaaagatga acggttgaaa taaaaatgca tttttccgct tgtcttcctg agccgactcc
    65461 ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac agccggttcg
    65521 gttgaagaga aaaaatcctg aaattcaggg ttgactctga aataggaaag tgtgatatac
    65581 gccacctcgc gacagtgagc tgaaagctgc gtcgcaactg ctctttaaca atttatcaga
    65641 caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacga
//
LOCUS       DAJVQR010000011        64148 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVQR010000011 DAJVQR010000000
VERSION     DAJVQR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            131..574
                     /locus_tag="OQM52_000736"
     CDS             131..574
                     /locus_tag="OQM52_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1562702.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="OQM52_000737"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="OQM52_000737"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCS1562703.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OQM52_000738"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OQM52_000738"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1562704.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OQM52_000739"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OQM52_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1562705.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OQM52_000740"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OQM52_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1562706.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OQM52_000741"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OQM52_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1562707.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="OQM52_000742"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="OQM52_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1562708.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="OQM52_000743"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="OQM52_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1562709.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="OQM52_000744"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="OQM52_000744"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1562710.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="OQM52_000745"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="OQM52_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1562711.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="OQM52_000746"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="OQM52_000746"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1562712.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="OQM52_000747"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="OQM52_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1562713.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="OQM52_000748"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="OQM52_000748"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1562714.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OQM52_000749"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OQM52_000749"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1562715.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="OQM52_000750"
     CDS             13934..14314
                     /locus_tag="OQM52_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1562716.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="OQM52_000751"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="OQM52_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1562717.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OQM52_000752"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OQM52_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1562718.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OQM52_000753"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OQM52_000753"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1562719.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="OQM52_000754"
     tRNA            complement(17415..17490)
                     /locus_tag="OQM52_000754"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="OQM52_000755"
     tRNA            complement(17494..17569)
                     /locus_tag="OQM52_000755"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="OQM52_000756"
     tRNA            complement(17621..17696)
                     /locus_tag="OQM52_000756"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="OQM52_000757"
     tRNA            complement(17699..17774)
                     /locus_tag="OQM52_000757"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17910..17985)
                     /locus_tag="OQM52_000758"
     tRNA            complement(17910..17985)
                     /locus_tag="OQM52_000758"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17950..17952),aa:Lys,seq:ttt)
     gene            complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="OQM52_000759"
     CDS             complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="OQM52_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1562720.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18951..19472)
                     /gene="pal"
                     /locus_tag="OQM52_000760"
     CDS             complement(18951..19472)
                     /gene="pal"
                     /locus_tag="OQM52_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1562721.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="OQM52_000761"
     CDS             complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="OQM52_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1562722.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="OQM52_000762"
     CDS             complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="OQM52_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1562723.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="OQM52_000763"
     CDS             complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="OQM52_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1562724.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="OQM52_000764"
     CDS             complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="OQM52_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1562725.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="OQM52_000765"
     CDS             complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="OQM52_000765"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1562726.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="OQM52_000766"
     CDS             complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="OQM52_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1562727.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="OQM52_000767"
     CDS             complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="OQM52_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1562728.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="OQM52_000768"
     CDS             complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="OQM52_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1562729.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="OQM52_000769"
     CDS             complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="OQM52_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1562730.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27906..30539)
                     /gene="mngB"
                     /locus_tag="OQM52_000770"
     CDS             complement(27906..30539)
                     /gene="mngB"
                     /locus_tag="OQM52_000770"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCS1562731.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30557..32473)
                     /gene="mngA"
                     /locus_tag="OQM52_000771"
     CDS             complement(30557..32473)
                     /gene="mngA"
                     /locus_tag="OQM52_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1562732.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32643..33365
                     /gene="mngR"
                     /locus_tag="OQM52_000772"
     CDS             32643..33365
                     /gene="mngR"
                     /locus_tag="OQM52_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1562733.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33468..34337)
                     /gene="sucD"
                     /locus_tag="OQM52_000773"
     CDS             complement(33468..34337)
                     /gene="sucD"
                     /locus_tag="OQM52_000773"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1562734.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34337..35503)
                     /gene="sucC"
                     /locus_tag="OQM52_000774"
     CDS             complement(34337..35503)
                     /gene="sucC"
                     /locus_tag="OQM52_000774"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1562735.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35597..36814)
                     /gene="odhB"
                     /locus_tag="OQM52_000775"
     CDS             complement(35597..36814)
                     /gene="odhB"
                     /locus_tag="OQM52_000775"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1562736.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36829..39630)
                     /gene="sucA"
                     /locus_tag="OQM52_000776"
     CDS             complement(36829..39630)
                     /gene="sucA"
                     /locus_tag="OQM52_000776"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1562737.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39931..40647)
                     /gene="sdhB"
                     /locus_tag="OQM52_000777"
     CDS             complement(39931..40647)
                     /gene="sdhB"
                     /locus_tag="OQM52_000777"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1562738.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40663..42429)
                     /gene="sdhA"
                     /locus_tag="OQM52_000778"
     CDS             complement(40663..42429)
                     /gene="sdhA"
                     /locus_tag="OQM52_000778"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1562739.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42429..42776)
                     /gene="sdhD"
                     /locus_tag="OQM52_000779"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42429..42776)
                     /gene="sdhD"
                     /locus_tag="OQM52_000779"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1562740.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42770..43174)
                     /gene="sdhC"
                     /locus_tag="OQM52_000780"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42770..43174)
                     /gene="sdhC"
                     /locus_tag="OQM52_000780"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1562741.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43555..43662
                     /locus_tag="OQM52_000781"
     CDS             43555..43662
                     /locus_tag="OQM52_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562742.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43868..45151
                     /gene="gltA"
                     /locus_tag="OQM52_000782"
     CDS             43868..45151
                     /gene="gltA"
                     /locus_tag="OQM52_000782"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1562743.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45542..46108
                     /locus_tag="OQM52_000783"
     CDS             45542..46108
                     /locus_tag="OQM52_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1562744.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46159..48618
                     /locus_tag="OQM52_000784"
     CDS             46159..48618
                     /locus_tag="OQM52_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1562745.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48633..49364
                     /locus_tag="OQM52_000785"
     CDS             48633..49364
                     /locus_tag="OQM52_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1562746.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49361..50437
                     /locus_tag="OQM52_000786"
                     /pseudo
     CDS             49361..50437
                     /locus_tag="OQM52_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50589..51635
                     /locus_tag="OQM52_000787"
     CDS             50589..51635
                     /locus_tag="OQM52_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1562747.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51632..52423)
                     /gene="nei"
                     /locus_tag="OQM52_000788"
     CDS             complement(51632..52423)
                     /gene="nei"
                     /locus_tag="OQM52_000788"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1562748.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52459..53193)
                     /gene="pxpA"
                     /locus_tag="OQM52_000789"
     CDS             complement(52459..53193)
                     /gene="pxpA"
                     /locus_tag="OQM52_000789"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1562749.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53183..54115)
                     /gene="pxpC"
                     /locus_tag="OQM52_000790"
                     /pseudo
     CDS             complement(53183..54115)
                     /gene="pxpC"
                     /locus_tag="OQM52_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54109..54765)
                     /gene="pxpB"
                     /locus_tag="OQM52_000791"
     CDS             complement(54109..54765)
                     /gene="pxpB"
                     /locus_tag="OQM52_000791"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1562750.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54788..55531)
                     /gene="ybgI"
                     /locus_tag="OQM52_000792"
     CDS             complement(54788..55531)
                     /gene="ybgI"
                     /locus_tag="OQM52_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1562751.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55802..57283
                     /gene="dtpD"
                     /locus_tag="OQM52_000793"
     CDS             55802..57283
                     /gene="dtpD"
                     /locus_tag="OQM52_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1562752.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57433..58851)
                     /gene="phrB"
                     /locus_tag="OQM52_000794"
     CDS             complement(57433..58851)
                     /gene="phrB"
                     /locus_tag="OQM52_000794"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1562753.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58848..59357)
                     /locus_tag="OQM52_000795"
     CDS             complement(58848..59357)
                     /locus_tag="OQM52_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1562754.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59486..59773
                     /locus_tag="OQM52_000796"
     CDS             59486..59773
                     /locus_tag="OQM52_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCS1562755.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59828..60208)
                     /locus_tag="OQM52_000797"
     CDS             complement(59828..60208)
                     /locus_tag="OQM52_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562756.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60211..63597)
                     /locus_tag="OQM52_000798"
     CDS             complement(60211..63597)
                     /locus_tag="OQM52_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1562757.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63616..63819)
                     /locus_tag="OQM52_000799"
                     /pseudo
     CDS             complement(63616..63819)
                     /locus_tag="OQM52_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15676 a        17272 c        15619 g        15581 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgca acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg
    17821 caggatgact cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca
    17881 cgttttacta ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac
    17941 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
    18001 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
    18061 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
    18121 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
    18181 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
    18241 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
    18301 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
    18361 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
    18421 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
    18481 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
    18541 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga
    18601 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
    18661 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
    18721 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
    18781 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
    18841 ctgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
    18901 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
    18961 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
    19021 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
    19081 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
    19141 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
    19201 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
    19261 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
    19321 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
    19381 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
    19441 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
    19501 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
    19561 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
    19621 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
    19681 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
    19741 accattggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
    19801 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
    19861 ctgcggacga ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt
    19921 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
    19981 cattacgtac aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
    20041 tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
    20101 atttgccagc gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
    20161 tttagaaccg tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac
    20221 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
    20281 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
    20341 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
    20401 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
    20461 attgtaagaa ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag
    20521 tgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
    20581 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat
    20641 atcttcaggt gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc
    20701 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
    20761 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
    20821 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
    20881 cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt
    20941 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
    21001 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
    21061 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
    21121 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
    21181 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
    21241 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
    21301 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
    21361 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
    21421 tgctgcctta tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
    21481 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
    21541 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    21601 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
    21661 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
    21721 tgcttcttcc gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt
    21781 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc
    21841 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
    21901 ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga
    21961 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
    22021 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
    22081 ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
    22141 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
    22201 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
    22261 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
    22321 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
    22381 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
    22441 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
    22501 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
    22561 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga
    22621 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
    22681 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
    22741 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg
    22801 ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg
    22861 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
    22921 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
    22981 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
    23041 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
    23101 tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
    23161 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
    23221 cacgcgccgc tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg
    23281 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
    23341 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
    23401 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
    23461 attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
    23521 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat
    23581 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
    23641 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
    23701 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
    23761 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
    23821 cgcgattgag gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga
    23881 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
    23941 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
    24001 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
    24061 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
    24121 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
    24181 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
    24241 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
    24301 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
    24361 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag
    24421 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
    24481 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
    24541 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
    24601 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc
    24661 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
    24721 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
    24781 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
    24841 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt
    24901 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
    24961 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
    25021 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
    25081 cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
    25141 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
    25201 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
    25261 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
    25321 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
    25381 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
    25441 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
    25501 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
    25561 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
    25621 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc
    25681 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
    25741 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
    25801 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag
    25861 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
    25921 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
    25981 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
    26041 cgtctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
    26101 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
    26161 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
    26221 ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca
    26281 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
    26341 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
    26401 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
    26461 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
    26521 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
    26581 gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga
    26641 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
    26701 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
    26761 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
    26821 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
    26881 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
    26941 gaccgtttcc ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa
    27001 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
    27061 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
    27121 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
    27181 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
    27241 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
    27301 gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct
    27361 ctttttgatg cccatttcca agacttatac attgataaat atcaatttct acaaattatg
    27421 ttaatcatgt ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata
    27481 agactgaaat tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac
    27541 ggttgacctt aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt
    27601 aataaaggca taaatctccc tcccgccgat attaacattt aattaactta taattatatt
    27661 ataattaact cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg
    27721 agagtgaaga taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct
    27781 ctgcaatata ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg
    27841 gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt
    27901 gctattcagg caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat
    27961 agatttgaac cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa
    28021 gaaatcacct cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta
    28081 aacagccgta aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat
    28141 cccactgggg gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg
    28201 agcttcatcg catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc
    28261 gcctgctgag ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga
    28321 caagaaagca gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc
    28381 ctaggcctta aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg
    28441 gcaaaggttt tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca
    28501 gccatgccgt tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc
    28561 ggcgcttctt tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc
    28621 gttagcgaac caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc
    28681 aggacgcgaa ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc
    28741 ctgttgttat gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt
    28801 gcgctgcgtt gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc
    28861 tgccaggcct catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac
    28921 tcttcttttg cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc
    28981 aacacccggt catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca
    29041 ttgagcgcaa tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca
    29101 atcacgttgc caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca
    29161 atctggttga tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt
    29221 tgccgatcga ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc
    29281 tgaccgcgat cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc
    29341 acttcttccc gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc
    29401 ggcatgttgt cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca
    29461 gccagttcga agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg
    29521 ctgtcgtggg catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg
    29581 tatccaaaac ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta
    29641 ttttcaatac gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc
    29701 acgcgcatat atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc
    29761 gcttcgatct tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa
    29821 atttcgcgca gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc
    29881 ccattcggca gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg
    29941 tcgaggcgtt tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc
    30001 aacggcatca cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt
    30061 ttatccgtac cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat
    30121 ccattgtaga tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt
    30181 ttcatcggct cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg
    30241 gattccgcag aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg
    30301 ccggcttcta ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct
    30361 tcgaggatcg ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt
    30421 cggcacagga tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa
    30481 taccactccc gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc
    30541 gtcgtttcct ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg
    30601 cgtgacgccg ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac
    30661 caaaccagcc aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg
    30721 agaaaatgcc agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg
    30781 ttaccataga gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg
    30841 ggatcgcccc ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa
    30901 tttcaaactc tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg
    30961 aaaccatttt gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc
    31021 ccaggcagaa tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa
    31081 aaccgagaat ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg
    31141 taagcgagtt attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg
    31201 ctcccaatgt accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc
    31261 taagacgcaa gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga
    31321 ccgcgccgag aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg
    31381 ctaacgccgg tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca
    31441 aaattccgag cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat
    31501 taaacagatc ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc
    31561 cccccgcgac aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct
    31621 ccgttttgac gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac
    31681 gctcgagggt aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag
    31741 gcacagaaag cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg
    31801 caaaaataca ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt
    31861 tagcaccttg cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat
    31921 actccgcagc catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag
    31981 ttggcgcgtt ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga
    32041 gcaattcatc aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc
    32101 gcgtggtcag cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg
    32161 ccagcaacac cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct
    32221 cgctgagtgt ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca
    32281 cagccaaccc ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca
    32341 gaaattgctc agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa
    32401 tggcctcttc acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag
    32461 tcgtcaggtt catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac
    32521 aaaacaatac cattgcgaaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta
    32581 ttgtttacag gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa
    32641 taatgggaca caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc
    32701 gtggcgagct taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg
    32761 gcgtcagccg ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg
    32821 aaagcattca gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc
    32881 agttaaccag ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc
    32941 tgatatttga agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc
    33001 aggatcgtgt ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg
    33061 aagaaacctg gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt
    33121 cgaaatatca ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa
    33181 tcattcctct gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc
    33241 cgattcttga aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc
    33301 gcaacgcttt taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc
    33361 gataagcaca aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc
    33421 cattcctgta gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca
    33481 gttttcagtg cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc
    33541 agagcagcga atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg
    33601 cccatacgtt tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta
    33661 acatgctctt tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc
    33721 atcacgatcg cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta
    33781 gagccaggga tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc
    33841 gtggtctgtt taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta
    33901 cccggtttgt gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg
    33961 cctgggcagt tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc
    34021 agcatatcca gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg
    34081 atagcttcca gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa
    34141 gcgccagtag cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta
    34201 gtgccgcctt tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt
    34261 tctgagtgga aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta
    34321 tcaattaaaa tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg
    34381 tcagaccttt tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt
    34441 cggcgttgtt accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg
    34501 cgccgatgat accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca
    34561 gaacggcttt cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt
    34621 tggttgcgcc gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta
    34681 tgtccatcgt acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca
    34741 gcgcaacgta gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact
    34801 ggtcacgcat ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt
    34861 tgccgtcgag gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca
    34921 gcgccaggtc gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct
    34981 gaaccagttt accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg
    35041 ggccagtcag cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt
    35101 tttcgatttc cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt
    35161 ctacaacggc accgagatac agctctttag cgatatcggt cgccgcttca accagaatct
    35221 ggttaaccgg ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt
    35281 tttctgcaaa agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac
    35341 cgcggccacc agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag
    35401 cggcttcttc tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat
    35461 agcgggcaaa cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt
    35521 cagtaatcgt tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca
    35581 ggtgaagctt aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt
    35641 tgatcgttac caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca
    35701 gcgccaggta catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt
    35761 tgatagcgtg catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca
    35821 gggaaccgaa cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg
    35881 tcagcttgcc atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca
    35941 tgccgagggt atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg
    36001 ccatgctgac gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca
    36061 cttccgggta acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca
    36121 gacggatgcc gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg
    36181 gcttcatgtt gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca
    36241 gacgctctgc cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag
    36301 ccagagccgg ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt
    36361 tcgccagatg tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa
    36421 tggcgctggc gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat
    36481 cgttgttttg ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt
    36541 cagatttggc gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct
    36601 gacgagacgt taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg
    36661 atgccggtac ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta
    36721 cgactgcgtc gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag
    36781 gcaggtcagg gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt
    36841 cagcgcgtca ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc
    36901 cggagaggcg gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc
    36961 acggaaatga tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca
    37021 gacaaaatcc ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa
    37081 cgggtagagt tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg
    37141 ttccagcagg tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg
    37201 atcaagctcg tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag
    37261 gctggaaacc gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg
    37321 acgcatcccg cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg
    37381 tacgcaaacc tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga
    37441 ggagtgctcc ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat
    37501 ccggccccat ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt
    37561 ggcgaagtca ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata
    37621 accatattca aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc
    37681 gccctgaccg ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg
    37741 gatcaccgcg tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac
    37801 cggaatgcct tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa
    37861 cagtttctca ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg
    37921 catttcaact gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat
    37981 ctcaactttg ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa
    38041 agagtgcatg ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc
    38101 gcggtacagg ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag
    38161 cttgtcagcg tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg
    38221 ctgggttgcg ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc
    38281 gatgaagaca tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc
    38341 aacggcttcc ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat
    38401 atcagtacag tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg
    38461 gttgttgata acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt
    38521 cagagtttcc tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg
    38581 cagcactttg ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat
    38641 aacgaccggg cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag
    38701 gccgccatcg gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc
    38761 gaggtgttct ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag
    38821 cacgttcacc agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt
    38881 gccgctgttg ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc
    38941 ttccagcgag aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc
    39001 agcggcggtc agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc
    39061 agactcgata cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc
    39121 ggcaccaatc gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat
    39181 ggtttctttg ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt
    39241 cagatcgtgg aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg
    39301 atctagattc gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt
    39361 aacctgcttc acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt
    39421 cgccaggcgg cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt
    39481 accaggtaac tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa
    39541 gaagtcttca tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc
    39601 caaccaggct ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt
    39661 acttcgccgt ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc
    39721 ttgtctacgt agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt
    39781 tttaaagatt tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg
    39841 gtaatgactt gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc
    39901 aacgcttgcg cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt
    39961 gatatggccg atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat
    40021 gatgctgtga cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt
    40081 ctcggtatca cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat
    40141 aaacttatcg ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat
    40201 acattcatac agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc
    40261 tggcggattt tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa
    40321 ttgtcccatg tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac
    40381 aatcttcttg cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt
    40441 gccgttcatg ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga
    40501 caggctggga tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc
    40561 ttcttccgct tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata
    40621 gcgataaatt gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt
    40681 cggcgggaat gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc
    40741 cgactcaggc agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc
    40801 gaagcggcta tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata
    40861 cgccgtttcc atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct
    40921 ggaagtgtca tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag
    40981 ccctttcgcc atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg
    41041 cagcgcttta cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag
    41101 gcgatccaga gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc
    41161 gatagactct tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga
    41221 gttgccgccc agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa
    41281 cagtcccgga acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac
    41341 tttggtcgga ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt
    41401 caccggatcg acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc
    41461 gagaacttct ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc
    41521 acagccgcga ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc
    41581 caggtctttg gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag
    41641 ataaccgcct tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt
    41701 cgggtggaat tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc
    41761 gacaccgtcg ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc
    41821 gccagtcgcc agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca
    41881 cagtgcggta caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca
    41941 ctcggagaaa atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc
    42001 gtgaccggta cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt
    42061 cgactgaccg ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag
    42121 gcccatgtgt tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc
    42181 gtcctggtca ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt
    42241 atcttcatgg gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga
    42301 acgggtcggg aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag
    42361 cgccgcgcgc atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg
    42421 caatttcatc acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca
    42481 acgacaatca ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac
    42541 acctgccaca tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac
    42601 actttggtga acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg
    42661 gtggcgaaaa aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga
    42721 acgaggatga aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga
    42781 ggactcctgc gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt
    42841 tacccgcttc gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta
    42901 cgacgacgtg atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc
    42961 tgcccataat cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa
    43021 gccactgcag gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg
    43081 acgctatcgc cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt
    43141 ttttcacatt tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact
    43201 acgggcacag aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct
    43261 gcttgcgggt cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag
    43321 atgcgaaaac gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc
    43381 tggcggcagt ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg
    43441 ttttatcctg aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct
    43501 aatttgacaa tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt
    43561 aaaagttatg aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg
    43621 accaattcat tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt
    43681 acttgattcg ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct
    43741 gtacccaggt tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca
    43801 ggttgatgtg cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag
    43861 accttaaatg gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact
    43921 ggatgtgctg aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa
    43981 aggtgtgttc acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac
    44041 ttttattgat ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc
    44101 gaccgattct aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca
    44161 ggaacagtat gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat
    44221 tacccgtctg ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat
    44281 taccggcgcg ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg
    44341 tgaaattgcc gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa
    44401 gtattccatt ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt
    44461 cctgaatatg atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg
    44521 tgctatggac cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac
    44581 cgtgcgtacc gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc
    44641 ttcactgtgg ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga
    44701 aattagctcc gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc
    44761 tttccgcctg atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt
    44821 aatgcgtgaa acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga
    44881 agtggctatg gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact
    44941 gtacccgaac gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc
    45001 catgttcacc gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga
    45061 aatgcacagt tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa
    45121 acgcgacttt aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta
    45181 acccgccgtt caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat
    45241 aaaatccact atcggacatt catatattct aaaaaaaaat acatcggtat aaattataaa
    45301 caaatagatg atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata
    45361 tagcgcaccc ctataattcc caatacttta ttcatcaaac atttaattca taccatcaat
    45421 taattacttt tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga
    45481 caatcaaata agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt
    45541 tatgtttaaa ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc
    45601 tgtttatgcg gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc
    45661 accgtgtgaa atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac
    45721 cagccacatt aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa
    45781 ctgtgatctg gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac
    45841 ctttgatagc tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg
    45901 tgaagccacc ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg
    45961 caccgctact ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt
    46021 tgcctggatg gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa
    46081 tgcaacctac gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc
    46141 ataaaatatt tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc
    46201 ctggtcgtgg cgatgccttg cgcattggcg gtcgaattca atctgaatgt tctcgataaa
    46261 tcaatgcgcg accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt
    46321 gagtattttg ttagcgttgc ggtaaataat aaccaaatca gtaacgagca aaagattaac
    46381 tggcacaaaa atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt
    46441 ggcttaaaac ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc
    46501 tcccgacctg aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt
    46561 ccgcaagcct ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa
    46621 ggtgtcgccg gtgtcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggat
    46681 ggcagcagca gcactaatct taacgcctat ggaacagccg gaatcaacac gggggcatgg
    46741 cgcttacgca gtgattatca gttgaatcat actgatagcg atgataacca tgaacagtca
    46801 ggcgaaatat cacgtaccta tctttttcgc ccgttaccgc aattaggttc aaaattaacc
    46861 ctcggcgaaa cggattttag tcccaatatt ttcgacggtt tttcttatac cggcgcggca
    46921 ctggcaagtg acgatcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc
    46981 ggtattgcac agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag
    47041 aaaaaagtcc caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca
    47101 ctggatgtca aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcagcagca
    47161 tcgacgccct tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca
    47221 cggccctcca tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg
    47281 gggatgctgt caaacacctc gctgtacagc ggcctgctgc tttctggtga tgactaccat
    47341 tctgccgcaa tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc
    47401 acgtgggcca gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt
    47461 aattacagca aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc
    47521 tccgatcgtc attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat
    47581 gcgcaggacg aaaaacagac gatcagctta tctgtgggcc aaccgattac cccactaaac
    47641 ctcaatcttt acgccaacct gctacatcaa acctggtgga atgcagacgc ctccacgacc
    47701 gccaacatca cggcaggttt taatgttgat attggtgact ggagagatat ctctatttct
    47761 acgtcattca atacaaccca ttacgaagat aaagatcgcg acaaccagat ttacctgtcg
    47821 atttcgctcc ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa cagtagccac
    47881 agcaccacac accgcatgtc gtggaacgat acgctggatg aacgtaatag ctggggcatg
    47941 tctgccggac tgcaatccga ccgtcctgac aatggagccc aggtgagcgg taactatcag
    48001 cacctgagtt cagcgggtga gtgggatatt tctggtacct atgccgccaa tgattacagt
    48061 tccgtcagca gcagctggag cggttctttc accgcaaccc aatatggtgc agcgtttcat
    48121 cgccgcagct ccaccaatga accacgcctg atggtcagca ccgatggcgt ggcagatatt
    48181 ccggttcagg gcaatctcga ctacaccaac cattttggca ttgcggtggt gccgttgatt
    48241 tccagttacc agccttccac cgtggcggtg aacatgaatg acttacccga cggcgtaaca
    48301 gttgcagaaa acgttatcaa agaaacgtgg attgaaggcg cgataggtta caaatcactg
    48361 gcttcccgtt ccggtaaaga cgttaacgtc atcattcgca acgccagcgg tcagtttcct
    48421 cccctcggag cggatatccg ccaggatgac agcggcatta gcgtggggat ggttggcgag
    48481 gaaggacatg cctggttaag cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt
    48541 gatagccagc attgctcgct ccatcttcct gaacatatgg aagacaccgc aaatcgcctg
    48601 attttacctt gtcattaatt aaggaaatga aaatgacatt tatgaaagga ctaccccttt
    48661 tgttgttggt tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg
    48721 tgtttaacgc taatgataaa gctaccagtc tgcgggtgga taatcgtagc gataaactcc
    48781 cctacctcgc ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg
    48841 tcgccctgcc gccgatacaa cgtctggaac cgaaagcaac aacacaggta cggatagtaa
    48901 agcaagcctc gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc
    48961 gtgaaattcc tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc
    49021 agagtcgtat caaggtgttc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg
    49081 tcgaactgca gttacaggtc agccagcagg gcaaccaact gacactgaaa aatcctaccg
    49141 cgtattatct gaccattgcc tacctgggac gaaatgaaaa aggcgttctc cccggcttca
    49201 aaaccgtgat ggttgcgcct tttagtacgg tcaacacaaa caccggaaat tatagcggca
    49261 gccagtttta tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaact
    49321 gcagcggaca atgccattta caagccgtgg aggcgaagaa atgagtgctg gcaagggagt
    49381 gttgctcgtc atctgtctgt tatttctgtc gttgaagtct gcgcttgccc tgaactgcta
    49441 ctttggttca tccggtggct cggtagaaaa atcagaagcg attcaacctt ttgctgtacc
    49501 aggcaatgcc aaactcggcg ataagatctg ggaatctgac gatattaaaa taagatctgg
    49561 gaatctgacg atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa
    49621 agcgaacatg tctacgtatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa
    49681 ctcggagtaa cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac
    49741 actaaccagt gtatcgacag taagaacatt gacatttata cccctgatgg gatggcagaa
    49801 caaaatttgc tcgggtgatc ccgctaatat tcacatgtcg cgaacctttc tcgctcgcat
    49861 gcggttatac gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga
    49921 ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa
    49981 cctgaaatat catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt
    50041 ttccatttca ccggaaacac agatgattga ttttggtaaa tttaatttgc tggatattcg
    50101 tcgccacaca atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg
    50161 caccgacgga tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga
    50221 taaagcattg ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc
    50281 ttataccttc aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga
    50341 aaaaacctat acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt
    50401 cgatacagtg gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc
    50461 cggaaagggc atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat
    50521 tttttcctag cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg
    50581 agcgaaggat gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta
    50641 ttggtttcct cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga
    50701 tcatcttcag tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc
    50761 aggccattct tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg
    50821 ccgccaactg gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg
    50881 tgggttggtt attggtgcgc tatagctcac tgcctggaaa taccggtgcc tggggttctt
    50941 ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc
    51001 tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca
    51061 cccgcatgat gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc
    51121 cagtgagcat taatctcctg cttacgattt tgctcgcagt cgttgccggt acggcgggat
    51181 gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc
    51241 agtctggtca gctcatcacc atcgaactac cggaatggct gctggcgatg gcgtatatgg
    51301 caattggctg gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc
    51361 cgctaccgca aatcctgctg tcgatttttg ctctgctggc tatttgtgca ggtatggcgt
    51421 ggggactgac ccggtttatg catattgatt ttatgactgc ctacctcgcc accagtcccg
    51481 gcgggcttga taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta
    51541 tggcgatgca aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt
    51601 ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga caccagtaaa
    51661 acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg cacagttcgc
    51721 catcacgatg aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct
    51781 gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca
    51841 gttgcgccgc attgagatct ttcgctttat gatttccagt caacccaacc tgccagagga
    51901 tctccacccg caaataattg ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg
    51961 caaactgacg gttacgaaag cgcggcgaca ataaccgttc ctttaccacc tccggcgtca
    52021 gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt
    52081 ccggcctcaa catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt
    52141 gcagttttac ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc
    52201 agacgccgta aagctgatta tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca
    52261 acgccttacc acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag
    52321 tttttaactg cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct
    52381 ccaggttatc cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc
    52441 attctttatt aagcggggtt atgctgcgac aacaatcccc tttttggcaa atgtagctcg
    52501 taatcggcgg gcgaacgcca gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc
    52561 cgcgaccgtt gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctccag
    52621 cgtttgcgcc aacgcctgtt cttcgttttc aatcaacgcg cctggctgac ttcgcggcac
    52681 cagcgagccg tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc
    52741 atattgcttg cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg
    52801 atcgcaagcg tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg
    52861 gttgtacaac atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat
    52921 cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa acggtttccg gcggcagctg
    52981 catggcgctg cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac
    53041 accatttttt attgcttcac gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa
    53101 tccacaagca atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc
    53161 ttcgcccaga tcggcgttca ggtcaatttt cattgtgcag ccgccacgct aattgttcaa
    53221 aataacgttg ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat
    53281 gaatcggctg accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg
    53341 caatacgtgg ataaccaccg gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt
    53401 tatgcggcac ctgcaccacg cccggtaaca aaccgtgaga taacagttcg cgatcggtga
    53461 tgcgttttaa aatttgcccc tgtaaacgat agcccattcg gttactttgc gagctaagct
    53521 gccagggcga acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc
    53581 ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa
    53641 aatcacgctt cgatttgcca atcgggagtc agtcaccatc cttcagtaaa cggccttcca
    53701 gcccgccaat ccccactttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa
    53761 taccacccgc gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac
    53821 gctggcctgc tttcatcggc aatcgccagc cggtccagac ggcgttatca tccaaccgcg
    53881 cttcgcaacc tgcacccgtc agggcaaacc acccatcagt ttcgaactca actgttaact
    53941 gaccgagcgt gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa
    54001 tgcgcagcgc aggcatatcc agtgcgccac agtggctgat acccgactgg caaaaaccgt
    54061 gacgaccgcc atcctgcaca gtggtgtaca tgcccgcacg aataatcttc agcatattcc
    54121 ctccttctgc ggtacaaatc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg
    54181 cgccggatca aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc
    54241 cagcggataa acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa
    54301 gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa
    54361 gcccggttga aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac
    54421 ctgtttttcg ctcaacccgc aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc
    54481 accgtaaacc accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc
    54541 ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat
    54601 caccgtgata ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg
    54661 ctgcgccagt cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag
    54721 cactaccgcc gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga
    54781 ttattcgtta tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat
    54841 tcagccactc gctcaatgcg cgaataccac cgcgttcagt ggcatggtga cctgcagcat
    54901 aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa
    54961 taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc
    55021 accaggcgac gcgctggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc
    55081 ccagacgcgc ttcaatccag gaggctagtt ccattcccgg cacaggcatg gtcagctctc
    55141 cccacggcac caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct
    55201 gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa atgccagcca tacaggttga
    55261 tatcattcgc cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact
    55321 ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct
    55381 catcgagcaa agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc
    55441 cttccacctg caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt
    55501 taatcagttg ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac
    55561 accgttaaca tagcgttaca tcgcctgttc gtcgatagcc aatcttttga catattaaga
    55621 aacaaacccg acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata
    55681 tttatgcaat cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt
    55741 aaggaaacac aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt gtggaattat
    55801 tatgaataaa cacgcatcac aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg
    55861 ggaatatttt agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact
    55921 aaaatacaac gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt
    55981 cacgccaatc ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat
    56041 gctgggggcg ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg agatccatcc
    56101 gtcattcctc tatctgtccc tggcgattat cgtctgcggc tatggcctgt ttaaatctaa
    56161 cgtaagctgt ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt
    56221 ctcgctgatg tatgcggcgg gtaacgtggg gtctattatc gcacctatcg cctgtggtta
    56281 cgcccaggaa gagtacagct gggcgatggg ctttggcctg gcggcggttg gaatgatcgc
    56341 gggtctggtc attttcttat gtggcaatcg tcatttcact catacccgcg gcgttaacaa
    56401 aaaagtactg cgtgcgacaa actttctcct gccgaactgg ggatggctgc tggttctgct
    56461 ggtggcaacg cctgcactga ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt
    56521 aattgtcgcg actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa
    56581 ccagaaacag cggaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt
    56641 ctgggccttc gcacaacagg gcggtagctc gattagcctt tatatcgacc gcttcgttaa
    56701 ccgcgatatg tttggttata ccgttccgac cgcgatgttc cagtcgatta atgccttcgc
    56761 agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg
    56821 taccgtgcgc atctggggaa aatttgctct tggccttggc ctgatgagcg ccggattctg
    56881 cattctgacc ttaagcgccc gctggtccgc aatgtatggt cactcttctc tgccactgat
    56941 ggtgttaggc ctcgcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc
    57001 gcaaattacg cgcattgaaa ttccgggcgt gaccggcgta ttaaccggca tctacatgct
    57061 gctttctggc gcgatcgcga actatctggc gggcgtgatt gccgatcaga catcgcaggc
    57121 ttcgtttgat gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga
    57181 tcaaattacc tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca
    57241 ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca taataggcca gcaccgatgc
    57301 ctgatgcgac gcttgacgcg tcttatcagg cctacgtatt tcctgcaatt tattgaattt
    57361 gcacaaattt gtaggctgga ttaggcgttc acgccgcatc cggcatctgg gctcgattgc
    57421 ctgacgcgtc tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct
    57481 cgcctctttg tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc
    57541 ccacttccac ggctcatgca ccgctttccc tggtacatcg cgcagctccg gtaaccatcg
    57601 acgaataaac tcgccctcac ggtcaaattt ctcgccctga gttatcggat tgaaaatacg
    57661 aaaatacggc gcagcatcag tacccgtaga agccgcccac tgccagccac cgttattagc
    57721 tgccaaatca ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat
    57781 caacaaatct ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc
    57841 agtgctgtta agctgacgca tagcggcatc aataatcggg tatcccgttt tgcctttctg
    57901 ccaggcctgt aaatgtgcgg gattgctttg ccactgcaca cgatccgtcc aggcaataaa
    57961 tggacgatgt ttacacaacg aggggtaata cgtcatcaga tgacggtaga actcgcgcca
    58021 gatcagctca ctaagccaga cactaccggc cccaccgtcc agcgcctgcg gctgttccgc
    58081 caacaagcga tgcaaacact ggcgaggcga taacccgccc gttgccaagc tggcggacaa
    58141 acggctggtg ccttccactg ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt
    58201 ctcgcaaaac tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt
    58261 atcgaaagac tgacgaggat aattcagcgt aatggatggc gcgggcttta tcgatccgct
    58321 actacgaact tttggtgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca
    58381 ggcattctta aaaggcgtaa agactttgta catctcgtga ttgccggtca tcaccgcccc
    58441 cggcggcagg atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc
    58501 aacgtccaca tcccgcgctc gctcattcac ctcatactga tagttataaa aaagatgagt
    58561 aacgctgttt tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac
    58621 ttcacggaac aataaaggaa tacctttttc cgccagcgcg atttgtagcc cattcagttg
    58681 agcattgata agttcagcct gacgcggcga catgttatgc gtctcccact tgcgtggtgt
    58741 agcgatatac agcgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag
    58801 attatcgtgc agacgtaaat cctggcgaaa ccaggccaga tgggtagtca taaaactcct
    58861 gaatggcgca agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag
    58921 gcgtcaggat aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc
    58981 tcgccacggc gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata
    59041 tgcgggcgaa aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg
    59101 ggatgagaaa gcaaaacaat tacgcgttgg cggtactggt tgtaaaaatc atccagattt
    59161 tgccactgat gaatatcagc cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg
    59221 gccagaaaga cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca
    59281 tgttctttca gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt gctgtcatca
    59341 aatcgttgta gattcattac tgctcctgaa cggtatacag gagtaagtgt aggcgttggt
    59401 aaatgatgtt tgtgaagtta atccgctagc agggcaagat tacgaaagcg cgctccccgc
    59461 aagcaactaa tcaaggtagt taatcatggc tgaacacgat attacgccag aagtcactat
    59521 ttccaaaaca cagcgccgct ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa
    59581 aaccggtata gcccggtatt attcacaaca tcccagtctg caccttaagg gcgactggct
    59641 gaaagaggcc ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct
    59701 gaccatcatc gctgacagtg acgaggtgca ggaactgcgg gatgggatgt ttagtgtgct
    59761 gaacgaaagt taaatattaa aaaagctggg aagatttaaa accttcctag ctttttattc
    59821 tgtcttatta taatggatac tcatcatctt ccgatgaatt tataaattgc tccaagttta
    59881 catgaagagt acttaaccta gtttttaact cattaagtga agcgataatt tcatattttc
    59941 caacataatc atatgatgga tcgtattcat tatctatgtc agaagctaaa aatgatgata
    60001 ttcgtagttt aaatttagct tcatctttat tacattcatt ggcataaggt gaatgcccta
    60061 tcatagcata ttcatctaat ctaaaaggat aagatggttt taattcatcg atcaatgaaa
    60121 tttcaatcca tgttctatat ccaggcatta atggactaat ttctttgagc aaaacctttc
    60181 cacttaataa tgtattgaga ttgttcatta ttatgtctcc cacgggaaaa atatatgtct
    60241 aacacttcca ccatgttgat gaacttgtaa atgtggtaaa tcaccatcag gatttcctgg
    60301 cccatgacct gacactctat atgaaggttt tgttacagaa ggatctttta tatcatccca
    60361 atgatatgaa cctgcttttg ttttattttc agtaagatat tccattaagt tcttagtgct
    60421 ggggcctgac tcacctgtca tatttttata attatgacct aaatatctat ccataaacaa
    60481 ttgttcagcg tcacttctag acctaacagt aactttggtt tcactactat ctaataaacg
    60541 attcgctttt tggttactaa cagcacatgg agttaatcca agtggatcag catattttag
    60601 aggattaggt gcataactgt agagatttaa accgcctctc aaccctatcg ggtcagttac
    60661 catgaaccgc ccgacatccg gatcatagta cctgtgcaga ttgtagtgca gccccgtttc
    60721 cctgtcgagg tactgtccct ggtagcgcag gttctgctcc accccgccgt ggtccttctc
    60781 ctgtatcgtg ttaccccaga cctgataccc cgtctcccac acgattttcc cgtcgctgtc
    60841 cgtcatctct tccggcgcgc cgttcacatc cgtgtgaaaa tacagtatcc ggttcgggcc
    60901 ctctttgccc gccttatcca cccgcgccag cggttcataa ctcccctggt ccctgtagat
    60961 gtacaggctg cctgttccct ccggcctgct ctcctgcgcc agccgcattc cgtcccataa
    61021 aaattttgtc cggttgtacg gcttaccgtc agggctgatg tgctgttttt ccgtccgcct
    61081 ccccagtgca tcgtacacat accggtatgt cccccgcgct gtcgtcactt ccaccatccg
    61141 gtgttccgca tcataacggt aactctgcgt tccgctccgg ccctcctttt ccaccgcacg
    61201 gccaaattcg tcataacggt agtgcacacc acggtaagac atcagccggt tgaacggcac
    61261 cacgctgttt tctgccgtgg ccctttcccc cgcgcgcctc cccagcaggt ttcccgccgc
    61321 gtccacctcc gtttccggct ggtgccacac cgggtgccac tgcgcctgct ccacccgccc
    61381 cagcgggtcg tgcgtctccg tgatatgccc ctgcaggtca tacacatagc gccatttgcc
    61441 gccctgaggg tcggtcatcc ccagcaacag tcgttcttcg tcgctccagc ggaatgtcgt
    61501 cacctgtccg gcttcgtcct ggtacgccgt cacctgattc tgcgcatccc actggtgata
    61561 acgtgtgcgc cccagtccgt ccgtcacgca cagcctgcgc tgcgccaggt cccacgccag
    61621 ccggtactgt tcaccgctgt ccgtgtggtg ccccgtcacc cgccatgccg cgtcatccgg
    61681 accttcatac cagccccact ggtaataata cccttcgccg cccggctggc ggtgcagcgt
    61741 catgcagtgg ttttcgtcgt aagcaaactc acgcagcagg tgccccgtgc tgtcgtacac
    61801 gccgttcagg tggccggcct cgtcatagct gtaacgacgc agcagtaaag gtgcagtctc
    61861 agggaaatgc tgataaatct gtgtcacccg ccggggatgc gccgccagtt cgtaatacag
    61921 acggatgcac ggacgctgct gttcgccgct gatttccaca atgcggcccc ggtcgtcgta
    61981 gtacagattc agacagttgc tgttacggtc gcccagcatt ttcagccgct ggcgctgcgg
    62041 gtggtgcggg tcagcctcaa acagaaaaca ctgcccgtca tcgctgctga taagccagtg
    62101 cccctgctca ccacgcctta ccgccagccc ttcaccgggg ctgtaaaacc cgccccccgg
    62161 cagtacggct tccatatcca agcgtcgccc catgcccgac acaaaggtca tgcagttttc
    62221 gtctgcgcta cccggtgtgc acacgaggct gacttcatac attacactcc agccctgacc
    62281 aaacattcct tccgtacgtt cgtcacgact gctgtacacg cgctgccagt ccagcggaaa
    62341 gtggcccggc agactgaaat cgaagtcatc gtcatcggcc aggtatttgg ccccggtggc
    62401 ggcattgacc ggatgcggaa gggcactggt ggccttctgc gtcagcatgc tcatcccgac
    62461 actggcagca aaacaggcga ttttagaaag cattttgccc gggcgaagaa acatcagcgc
    62521 cacggttgcc accagggtta tctgcgacct gccgcttctg atatccctga ccaccagtaa
    62581 cggaccgcca atgcgcacat ccggtgacac aaacgccccg ttccccggct cattcaccac
    62641 cctggcctcg caggtacagc gtgccccgct gcgtgtcgcc ggctggctgt tgatacgcac
    62701 atcccgcgcg ccttccgcga tatattccgc tgcgggcggc tcgtcatcgc ctttgaacag
    62761 attactgatg ctgccaataa ccgcattcac tccctgaaag gcggtttcca gcataccgcc
    62821 cggattcagc aggttaaggg ccgttcccag ggggtcatcc agaaaggcgg cttttttttg
    62881 tgcgagaaac tgcgtcagtg aggaaggatg tttatggcag tcgattttat cgtcaggggt
    62941 cggggtggat cccggggccg cactggccac agtgggctgc catagttccg acacaaaatg
    63001 tgtcacgcca tccaccattt cagaacgccc ttcttccgta ctgagctggt cgtgcagagc
    63061 ccgggacatc agcacaggcc ctgtcaccgg gttaaccgcc agggctatgg cccccgccat
    63121 ttccagatat tccccggcca catcccggaa cgttctggca tcctcctgac gctgcgcttc
    63181 ttcctgttct gccttcgcct gcgcttcctg cgcctctatg tcctgccgcg atgccgcagt
    63241 cgccgctgcc cgcgcggcag gtttgctgtt gatccatgtg tcaccagagc cggtgaggat
    63301 gtaaccttca atctgaggcg ggaaaagcat atttgcgagg gctgaacagg cttcgctgac
    63361 cttttctgtc acgccggtgt acaccatcgc cacacccgcc acaaaaccgg tcagcacgac
    63421 ggccgtgcac cccatcgtga tccccactga cgtcgccagt aacgaggttg tgccgacaat
    63481 gagggcatca ataacgacat ttgcggccac ctccagcacc gcccccagca catctgccat
    63541 aaacgacgta tgcagcaggg catcaccctg tcttgccgcc cagaatgcct cttccatgtc
    63601 ccgttccctg tatgattaat tcgtcctgac ttcctgccgc atcctgaagc gcagattctt
    63661 tttaatttca gcccagcgcg tcatatctgc cggggtcagc ggtgtggttc tggcgtaggt
    63721 gaaggccatc atgacccgct cacccggcaa tacaaatgcc acctgacact ggtacacctg
    63781 ctcgttaccc cggagaaact ggctctgtat ttccaaaccg cctcgttgag ataatattcc
    63841 gtcataatgt aaatagtccg ttttaataaa ggaattattt attgacaggt tctgtttttt
    63901 taatcatgaa tgtaataccc atcaccgctt tacagcacac tttatctttc cagtcataat
    63961 cactaataat attaagcaat tagcaatatt tacaacgacc agagcggata catagttatg
    64021 tatttaacaa tcccaatata ttcactatgc aaataaaacc actagtaaca gacacgctac
    64081 cgcctccata taaaaatgga caccttataa aattgtatct tgcatagaaa aagctgaaat
    64141 atcggatg
//
LOCUS       DAJVQR010000012        60413 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVQR010000012 DAJVQR010000000
VERSION     DAJVQR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..316
                     /locus_tag="OQM52_000800"
     CDS             <1..316
                     /locus_tag="OQM52_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1562758.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="OQM52_000801"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="OQM52_000801"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1562759.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="OQM52_000802"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="OQM52_000802"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1562760.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OQM52_000803"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OQM52_000803"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1562761.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="OQM52_000804"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="OQM52_000804"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1562762.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="OQM52_000805"
     CDS             complement(3720..4949)
                     /locus_tag="OQM52_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1562763.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="OQM52_000806"
     CDS             complement(5013..5453)
                     /locus_tag="OQM52_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1562764.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="OQM52_000807"
     CDS             complement(5558..6328)
                     /locus_tag="OQM52_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1562765.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="OQM52_000808"
     CDS             complement(6325..7251)
                     /locus_tag="OQM52_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1562766.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="OQM52_000809"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="OQM52_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1562767.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OQM52_000810"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OQM52_000810"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1562768.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="OQM52_000811"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="OQM52_000811"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1562769.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OQM52_000812"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OQM52_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1562770.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="OQM52_000813"
     CDS             12958..13305
                     /locus_tag="OQM52_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1562771.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="OQM52_000814"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="OQM52_000814"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1562772.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="OQM52_000815"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="OQM52_000815"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1562773.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="OQM52_000816"
     CDS             complement(15125..15487)
                     /locus_tag="OQM52_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1562774.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OQM52_000817"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OQM52_000817"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1562775.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="OQM52_000818"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="OQM52_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OQM52_000819"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OQM52_000819"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1562776.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OQM52_000820"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OQM52_000820"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1562777.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OQM52_000821"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OQM52_000821"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1562778.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OQM52_000822"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OQM52_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1562779.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="OQM52_000823"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="OQM52_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1562780.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OQM52_000824"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OQM52_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1562781.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OQM52_000825"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OQM52_000825"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1562782.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="OQM52_000826"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="OQM52_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1562783.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="OQM52_000827"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="OQM52_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1562784.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="OQM52_000828"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="OQM52_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="OQM52_000829"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="OQM52_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1562785.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OQM52_000830"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OQM52_000830"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1562786.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="OQM52_000831"
     CDS             complement(36342..36380)
                     /locus_tag="OQM52_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562787.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="OQM52_000832"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="OQM52_000832"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1562788.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="OQM52_000833"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="OQM52_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1562789.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="OQM52_000834"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="OQM52_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1562790.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OQM52_000835"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OQM52_000835"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1562791.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OQM52_000836"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OQM52_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1562792.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OQM52_000837"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OQM52_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1562793.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OQM52_000838"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OQM52_000838"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1562794.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OQM52_000839"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OQM52_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1562795.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OQM52_000840"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OQM52_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1562796.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OQM52_000841"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OQM52_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1562797.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OQM52_000842"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OQM52_000842"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1562798.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OQM52_000843"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OQM52_000843"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1562799.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OQM52_000844"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OQM52_000844"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1562800.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OQM52_000845"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OQM52_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1562801.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OQM52_000846"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OQM52_000846"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1562802.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OQM52_000847"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OQM52_000847"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1562803.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OQM52_000848"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OQM52_000848"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1562804.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OQM52_000849"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OQM52_000849"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1562805.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OQM52_000850"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OQM52_000850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1562806.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OQM52_000851"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OQM52_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1562807.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OQM52_000852"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OQM52_000852"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1562808.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OQM52_000853"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OQM52_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1562809.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14471 a        16732 c        14845 g        14365 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgtccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcaca agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcaccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcagaca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccgac cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacctcctt gatgtaaaca aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg tctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt caacaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggccccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggta attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgagtccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcacat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgagcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DAJVQR010000013        59656 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVQR010000013 DAJVQR010000000
VERSION     DAJVQR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="OQM52_000854"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="OQM52_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1562810.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="OQM52_000855"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="OQM52_000855"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1562811.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="OQM52_000856"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="OQM52_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1562812.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OQM52_000857"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OQM52_000857"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS1562813.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OQM52_000858"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OQM52_000858"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1562814.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="OQM52_000859"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="OQM52_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1562815.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="OQM52_000860"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="OQM52_000860"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1562816.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="OQM52_000861"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="OQM52_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="OQM52_000862"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="OQM52_000862"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1562817.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="OQM52_000863"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="OQM52_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1562818.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OQM52_000864"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OQM52_000864"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1562819.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="OQM52_000865"
     CDS             complement(13538..13933)
                     /locus_tag="OQM52_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1562820.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OQM52_000866"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OQM52_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1562821.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="OQM52_000867"
     CDS             complement(15220..15447)
                     /locus_tag="OQM52_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1562822.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="OQM52_000868"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="OQM52_000868"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1562823.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="OQM52_000869"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="OQM52_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1562824.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OQM52_000870"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OQM52_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1562825.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="OQM52_000871"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="OQM52_000871"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1562826.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="OQM52_000872"
     tRNA            19688..19764
                     /locus_tag="OQM52_000872"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="OQM52_000873"
     CDS             complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="OQM52_000873"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1562827.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21324..22655
                     /gene="argA"
                     /locus_tag="OQM52_000874"
     CDS             21324..22655
                     /gene="argA"
                     /locus_tag="OQM52_000874"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1562828.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22717..24543)
                     /gene="recD"
                     /locus_tag="OQM52_000875"
     CDS             complement(22717..24543)
                     /gene="recD"
                     /locus_tag="OQM52_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1562829.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24543..28085)
                     /gene="recB"
                     /locus_tag="OQM52_000876"
     CDS             complement(24543..28085)
                     /gene="recB"
                     /locus_tag="OQM52_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1562830.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="OQM52_000877"
     CDS             complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="OQM52_000877"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1562831.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31142..34510)
                     /gene="recC"
                     /locus_tag="OQM52_000878"
     CDS             complement(31142..34510)
                     /gene="recC"
                     /locus_tag="OQM52_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1562832.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34523..34882)
                     /locus_tag="OQM52_000879"
     CDS             complement(34523..34882)
                     /locus_tag="OQM52_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1562833.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVLMVMIVTALSG
                     IQRTSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVS
                     PGGREGEMTRLHCPNCQ"
     gene            complement(34867..35274)
                     /locus_tag="OQM52_000880"
     CDS             complement(34867..35274)
                     /locus_tag="OQM52_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1562834.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35271..35834)
                     /locus_tag="OQM52_000881"
     CDS             complement(35271..35834)
                     /locus_tag="OQM52_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1562835.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35825..36295)
                     /locus_tag="OQM52_000882"
                     /pseudo
     CDS             complement(35825..36295)
                     /locus_tag="OQM52_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36479..37273)
                     /gene="thyA"
                     /locus_tag="OQM52_000883"
     CDS             complement(36479..37273)
                     /gene="thyA"
                     /locus_tag="OQM52_000883"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1562836.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37280..38155)
                     /gene="lgt"
                     /locus_tag="OQM52_000884"
     CDS             complement(37280..38155)
                     /gene="lgt"
                     /locus_tag="OQM52_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1562837.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38306..40552)
                     /gene="ptsP"
                     /locus_tag="OQM52_000885"
     CDS             complement(38306..40552)
                     /gene="ptsP"
                     /locus_tag="OQM52_000885"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1562838.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40565..41095)
                     /gene="rppH"
                     /locus_tag="OQM52_000886"
     CDS             complement(40565..41095)
                     /gene="rppH"
                     /locus_tag="OQM52_000886"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1562839.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41780..42469
                     /gene="mutH"
                     /locus_tag="OQM52_000887"
     CDS             41780..42469
                     /gene="mutH"
                     /locus_tag="OQM52_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1562840.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42538..43251
                     /locus_tag="OQM52_000888"
     CDS             42538..43251
                     /locus_tag="OQM52_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1562841.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43389..43607
                     /gene="ygdR"
                     /locus_tag="OQM52_000889"
     CDS             43389..43607
                     /gene="ygdR"
                     /locus_tag="OQM52_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1562842.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43715..44755
                     /locus_tag="OQM52_000890"
     CDS             43715..44755
                     /locus_tag="OQM52_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1562843.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44788..45978)
                     /gene="lplT"
                     /locus_tag="OQM52_000891"
     CDS             complement(44788..45978)
                     /gene="lplT"
                     /locus_tag="OQM52_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1562844.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45971..48130)
                     /gene="aas"
                     /locus_tag="OQM52_000892"
     CDS             complement(45971..48130)
                     /gene="aas"
                     /locus_tag="OQM52_000892"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1562845.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48716..49747
                     /gene="galR"
                     /locus_tag="OQM52_000893"
     CDS             48716..49747
                     /gene="galR"
                     /locus_tag="OQM52_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1562846.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49754..51016)
                     /gene="lysA"
                     /locus_tag="OQM52_000894"
     CDS             complement(49754..51016)
                     /gene="lysA"
                     /locus_tag="OQM52_000894"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1562847.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51138..52073
                     /gene="lysR"
                     /locus_tag="OQM52_000895"
     CDS             51138..52073
                     /gene="lysR"
                     /locus_tag="OQM52_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1562848.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52060..52752)
                     /gene="ygeA"
                     /locus_tag="OQM52_000896"
     CDS             complement(52060..52752)
                     /gene="ygeA"
                     /locus_tag="OQM52_000896"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1562849.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52881..54299)
                     /gene="araE"
                     /locus_tag="OQM52_000897"
     CDS             complement(52881..54299)
                     /gene="araE"
                     /locus_tag="OQM52_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS1562850.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54614..55375)
                     /gene="kduD"
                     /locus_tag="OQM52_000898"
     CDS             complement(54614..55375)
                     /gene="kduD"
                     /locus_tag="OQM52_000898"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1562851.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55405..56241)
                     /gene="kduI"
                     /locus_tag="OQM52_000899"
     CDS             complement(55405..56241)
                     /gene="kduI"
                     /locus_tag="OQM52_000899"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1562852.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56528..57709)
                     /locus_tag="OQM52_000900"
     CDS             complement(56528..57709)
                     /locus_tag="OQM52_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1562853.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57964..59193
                     /locus_tag="OQM52_000901"
                     /pseudo
     CDS             57964..59193
                     /locus_tag="OQM52_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14729 a        16014 c        14519 g        14394 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgaa gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct
    19801 gatctcccat aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt
    19861 gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg
    19921 aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
    19981 agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt
    20041 ttatgcagct tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg
    20101 tcagcaatag tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg
    20161 cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc
    20221 gcagtactgg ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga
    20281 ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt
    20341 gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
    20401 ttgccctctt cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt
    20461 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
    20521 ataatgacaa tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc
    20581 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
    20641 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
    20701 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt
    20761 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
    20821 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
    20881 acgcgttcag ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
    20941 gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca
    21001 gccaggctga cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga
    21061 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
    21121 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
    21181 atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc
    21241 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa
    21301 ataatcatgc aaaggggtgt accgtggtaa aggaacgtaa aaccgagctg gtcgagggat
    21361 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
    21421 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
    21481 acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc
    21541 tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa
    21601 cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt
    21661 caatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt
    21721 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
    21781 tccggcggat tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa
    21841 tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg
    21901 ccactcaact agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg
    21961 gcgttactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac
    22021 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
    22081 gcgcagtgaa agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag
    22141 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
    22201 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga
    22261 ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg
    22321 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
    22381 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
    22441 gcagttcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg
    22501 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
    22561 ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc
    22621 gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca
    22681 ggcgtaacac tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc
    22741 cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc
    22801 ggcatacagc gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt
    22861 tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg
    22921 cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac
    22981 agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag
    23041 cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac
    23101 cggtcggcct tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat
    23161 aaactgttca attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca
    23221 caaaagctgg tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag
    23281 cagatccaga taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc
    23341 gccgctctgt aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac
    23401 tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc
    23461 gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc
    23521 ggttcctgcc ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt
    23581 aaagcccgcg ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc
    23641 cagttgatca cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag
    23701 tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg
    23761 cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg
    23821 gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg
    23881 tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc
    23941 cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc
    24001 cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag
    24061 cgccactgcc gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc
    24121 cagggtttgc gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa
    24181 gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag
    24241 gatcatcggc gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc
    24301 ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc
    24361 gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa
    24421 cagtgccgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg
    24481 cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt
    24541 cattacgcct cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg
    24601 gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg
    24661 aacagataga taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc
    24721 agataacgat gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc
    24781 atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat
    24841 ttatagtcga gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt
    24901 aacatgccgc gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac
    24961 tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac
    25021 tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg
    25081 agaggtgcct ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca
    25141 aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg
    25201 aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc
    25261 ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt
    25321 ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca
    25381 cgccagttat cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca
    25441 tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca
    25501 tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc
    25561 agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg
    25621 cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa
    25681 agcaaacgca gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca
    25741 gcattaagat ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg
    25801 acgcggaaat tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta
    25861 tggatcgtga caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag
    25921 gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt
    25981 tccagctgcg ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc
    26041 cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc
    26101 cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg
    26161 actaccgcat cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc
    26221 cccatcattg acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc
    26281 gcctgcaaca accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc
    26341 gaaaggtaaa cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc
    26401 tggcggctgc gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg
    26461 ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca
    26521 catacctgcg ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc
    26581 cacattttca tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc
    26641 ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg
    26701 ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa
    26761 gtgtagtggg catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg
    26821 aatgcatata tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg
    26881 tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc
    26941 attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc
    27001 agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt
    27061 tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc
    27121 gacaatggtt ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc
    27181 cccccggcct tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc
    27241 aactgataac tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc
    27301 tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt
    27361 tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg
    27421 tggcgggaag ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct
    27481 tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg
    27541 acctgggcta tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc
    27601 tggtagcgta gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca
    27661 aaggcattca ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca
    27721 tccatctgcc gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct
    27781 tccagcaggc gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac
    27841 tcgtggatat tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg
    27901 accaccagca gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca
    27961 agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct
    28021 tcaatcaggc gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca
    28081 ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac
    28141 tttccagcct tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga
    28201 cagaatagcc atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg
    28261 cggcgtcagc agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc
    28321 gcgatcgaaa tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag
    28381 catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa
    28441 ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt
    28501 atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc
    28561 aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac
    28621 gatctgcccc aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac
    28681 aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg
    28741 ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg
    28801 tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat
    28861 aaactctggt cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt
    28921 aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat
    28981 ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg
    29041 gttataccag gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc
    29101 ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat
    29161 aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa
    29221 ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc
    29281 gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc
    29341 aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg
    29401 atggtcgtat ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag
    29461 ctctggcaaa gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc
    29521 gctgatttta tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc
    29581 tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt
    29641 tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg
    29701 aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg
    29761 ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc
    29821 tttttcacgt aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc
    29881 caggccttta tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat
    29941 aggatcggag ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag
    30001 tgtacctggg ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa
    30061 cttcgctgag ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg
    30121 gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg
    30181 ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc
    30241 cggcagcggt ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc
    30301 gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag
    30361 gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg
    30421 tgccatgcgc atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg
    30481 ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc
    30541 taccgcaccg ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc
    30601 agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg
    30661 cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta
    30721 cgcctcggga tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc
    30781 ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg
    30841 gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc
    30901 ctgacttaat ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg
    30961 gggcattcac ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca
    31021 aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc
    31081 agtccgtgac tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca
    31141 ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg
    31201 cctccattgt ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt
    31261 cgccacgcac catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca
    31321 acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg
    31381 cgccgccact ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa
    31441 tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac
    31501 cgtctttgcg tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt
    31561 gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca
    31621 ggccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat
    31681 caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca
    31741 tctcctggca ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg
    31801 ccgcccggaa gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata
    31861 actgctgatt gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga
    31921 tttcgctgtc ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg
    31981 gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca
    32041 gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac
    32101 gagcatagct ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat
    32161 ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat
    32221 cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg
    32281 ggaaacgttc actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct
    32341 gcgcggaaat taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc
    32401 cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa
    32461 cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca
    32521 gagtacaaat gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac
    32581 gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta
    32641 aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt
    32701 ccgcatccgg caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta
    32761 gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca
    32821 gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc
    32881 actcgccctg cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc
    32941 aggtgtgttg tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc
    33001 aacgaatgcc ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt
    33061 caaaccgcgc cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc
    33121 gactgtcagg cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg
    33181 cacgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg
    33241 cctgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag
    33301 taagtgtcgg ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac
    33361 gctgcgggct atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt
    33421 tgttatcgct acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt
    33481 ccagaatgtc agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt
    33541 ccagctcctg gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc
    33601 cccatgaggc cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc
    33661 cggcattttc gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct
    33721 gacgagtcag cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc
    33781 aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga
    33841 gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg
    33901 ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc
    33961 agcgcggttg tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc
    34021 aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt
    34081 ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca
    34141 gttttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct
    34201 ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc
    34261 tctctttggg gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca
    34321 gcggaaaatc aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact
    34381 gcgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt
    34441 cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga
    34501 cccttaacat agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc
    34561 cttctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg
    34621 tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct
    34681 gccaaccatg ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg
    34741 ttcgctggat acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca
    34801 gcaccatcag caccatcagc accatcgcaa ccattacctc cggcaggcta aagccttgtt
    34861 gattcttcag ggaagctgac ataatgcccc ctctttcagc ggacaaaaat cgctccagcc
    34921 gcgtggcgaa aaaacaattt ttccatcaat gacttcgcct gttcgccaca acgaaacgcc
    34981 ttcataaccg gcaatcaata aggcttcatt atctgccagt aaacgcaaac aaacctgggc
    35041 atcggttcca gcgtactgcg agcactgaac tgctggctgc gtctgccagg agtgcatttt
    35101 tccccacgcc agcgccgact gaacgatggc ctggcggcgc aatgactgac tttccatgct
    35161 cacgcgagag gcaaaactgc gatcctgctg actcattcct tgcaatagca agctacccaa
    35221 aaccagcagc atcaggacca gagccagtga cgaaacacct ttttcgcggt tcacaggttg
    35281 aatcctgtca cgctatagct ggcatccacc acggtttgcg gttcagactt actggcagca
    35341 cgcatattaa ccgtcaacac cggcgagaag ccgctgacat cctgacgtac gacctgaaaa
    35401 gtgtcgataa tgatggcatc cggattagtg actttatccc agcccttacc ttcacaggat
    35461 gtcgcaccgc gtagcgtttc cagcacatgc tccttcagac gaaatccaat ctggtcggac
    35521 tcttttaccg gttcgcgatc ccagataccg ttactgttcg catcccactg cacaatgata
    35581 cagtcaccct gtccgacaat ttccagccct tcgccggtac agctgccatg acaataaccc
    35641 gccctctgga gatgcttcgc gacggtaaat acccgcagcc agatttcatc ttccagtgcc
    35701 agcttacgag tgctcgttaa actttcacgc tgtaacgcag gcagaaagcg tgcagccccc
    35761 agcaacaata cgctactgat cgccatagca atcaacactt ccagcagaga aaaaccttgc
    35821 tcttttacag gcatccttct gtttctcctt gctgataaag ccggagtctt ccccacggcg
    35881 aaaccaccag ccaccactcg cccgttgagt ttttgaagcg aatatgcacg gcccatgcgg
    35941 tattgcgcag gccaaagaaa gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc
    36001 agcgtggcac aaagaccaat ggtgaactgc catgacaggt attggcccca gcagcggaac
    36061 tcacaaggca ccataacgtc ccctccctga taacgctgat actgtggtcg cggttatgcc
    36121 agttggcatc ttcacgtaaa tagagcaaat agtcccgcgc ctagctggcg gtttgccaaa
    36181 gccgttgcga ctgctgccag tattgccagc catagagtcc acttgcgctt agcatgacca
    36241 aaatcagcat cgcgaccagc gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg
    36301 cagtatggag cgaggagaaa aaaagacgag ggccagtttc tatttcttcg gcgcatcttc
    36361 cggactattt acgccgttgc aggacgttgc aaaatttcgg gaaggcgtct cgaagaattt
    36421 aacggagggt aaaaaaaccg acgcacactg gcgtcggctc tggcaggatg tttcgtaatt
    36481 agatagccac cggcgcttta acgcccggat gtggatcgta gccttcaatc tcaaagtctt
    36541 cgaaacggta gtcgaagatg gattcgggtt tacgtttgat aatcaacttc ggcagcggac
    36601 gcggttcgcg gcttaattgc agatgagttt gatccatatg gttgctgtac aggtgcgtat
    36661 cgccaccggt ccagacaaaa tcacccactt ccagatcgca ctgctgcgcc atcatatgca
    36721 ccagtaacgc gtagctggca atgttgaacg gcaggccgag gaagacgtca caggagcgct
    36781 gataaagctg gcaagagagt ttgccgtctg ccacatagaa ctggaagaat gcatggcacg
    36841 gtgccagcgc cattttatcc agttcgccta cgttccacgc tgaaacaata atgcggcgcg
    36901 aatccgggtc gtttttcagc tggttcagta ccgtagtaat ctggtcaata tgacgaccat
    36961 ctggcgttgg ccaggcgcgc cactgtttac catacactgg cccgaggtcg ccattttcat
    37021 cggcccattc gtcccagatg gtgacattgt tttcgtgtag ataagcaatg ttagtatcgc
    37081 cctgcaggaa ccacagcagt tcgtggatga tggaacgcag gtggcaacgt ttggttgtca
    37141 ccagcgggaa tccatcctgc aggttaaaac gcatctgatg accaaaaatg gaaagcgttc
    37201 cggttccggt acggtcgttt ttctgtgtgc cttcgtcgag cactttttgc atcagttcta
    37261 aatactgttt catggttcct caggaaacgt gttgctgtgg gctgcgacga tatgcccaga
    37321 ccatcatgat cacacccgcg acaatcatcg ggatggaaag aatttgcccc atgctgatgt
    37381 actgcaccca ggcaccggta aactgcgcgt cgggctggcg gaaaaactca acaatgatgc
    37441 gaaacgcgcc gtagccaatc aggaacaagc ctgagacagc tcccattggg cgcggtttac
    37501 gaatatacag gttgaggata ataaacagca ccacaccttc cagcagcagt tcgtaaagct
    37561 gtgatgggtg gcgcggcagc acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg
    37621 tttgcagcag caaaatatct tctgtacggg agcctgggaa cagcatggca aacgggaagt
    37681 tcgggtcaac gcggccccac aattcaccgt taataaagtt gcccagacgc ccggcaccaa
    37741 gaccaaacgg aatgagtggt gcgataaaat cagagacctg gaagaaggaa cgtttagtac
    37801 ggcgggcgaa gataatcatc accacgataa cgccaatcag gccgccgtgg aaagacatgc
    37861 cgccgtccca gacacggaac agatacagcg gatcggccat aaactgcggg aaattgtaga
    37921 acagaacata accaatacgt cccccgagga agacgccgag gaagcccgca tagagtaagt
    37981 tttcaacttc atttttggtc cagccactgc ccggacgatt cgcccgacgt gttgccagcc
    38041 acattgcaaa aatgaacccc accagataca tcaggccgta ccagtgaagc gccacgggtc
    38101 ctattgagaa aatgaccgga tcaaactccg gaaaatgcag atagctactg gtcatctgtc
    38161 accacaagtt cttgttattt cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt
    38221 tcaggcgctc caaaggtgcg aataatagca caaggggacc tggctggttg ccggataccg
    38281 ttaaaagata tgtatatgat ccgcgctata accctccgcg aatcagcccg cccatgccgc
    38341 gacgctccat aaaggctgca acctgatgac gaacttcggt cgccagttgc gcttccagac
    38401 tacgctgcgc aagattttct gcttcggcaa aatcaatgcg ccgcagcagg tattttaccc
    38461 gcgctacaga acgtccgttc atagacagat ggcgataccc aagcccaatg aggattgcca
    38521 cgcacatggg atcgcccgcc atttcaccgc acaaacgaag atcgatacca tgtatttccg
    38581 cttcccgggc gatcatcgcc agagctcgta acattgcagg atgaagactg tcgtaaatgt
    38641 tcgccacccg ggtattgttg cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc
    38701 caacagagat gaaatcgacc ctctttgcca gatgcggcag cataaatacc attgacggca
    38761 cttccagcat gatgccgata cgtggtttgg gaatttcgta accgatcatc tcctcgactt
    38821 cacgtccggc acgttcaatc aggcggcgtg cttcgtcaac ttcatcgagg ctggtgacca
    38881 tcggcaacag aatattcagg ttgcccgtag cggcattagc acgcagcatc gcccgcacct
    38941 ggatcaagaa gatctccggc tgatcgagcg taatgcgaat cccacgccaa cccaggcatg
    39001 gattctcttc gctgatcggc atgtaaggca gctgcttatc tgctccgaca tccagcgtac
    39061 gcaaggtgac gggtttatca ttgaacattt gcagcatccc ctgatactgc gccacctgtt
    39121 cttcttccga cgggaaacca ctttgcagca tgaatgggat ttcagtgcga taaagaccga
    39181 tgccatcaat acagctgccc agtttttctt catgttccgg gcttaaacca gcattgagca
    39241 tgacttttat acgctcaccg ctttttaact gcgcgggtaa attgacgtca tcttccgcca
    39301 gacggctaag ctcaatctct tcactaatta gccgctgata ttcttgcagc agtaccggct
    39361 ccggatcgac cagcaattca ccgcgatagc catcaacgat cagcgtccta cgatgcagca
    39421 ccgaaggctg aatatccgcg cccatcacgg tagggatccc cagcgcacgt accatgatcg
    39481 cagcatggga gttggctgcg ccatctcgca cgacaacacc gactaagcga tcctggggca
    39541 gctcagcaag agttgtcgct gacagttcat ctgccaccag aatgaaacgt tccggccagg
    39601 cgttcggccc ttgattagcg tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta
    39661 aatcaccagc ccgctctttg agatagttat cgcttagcgc ggcaaactgt tcggcaaatt
    39721 tttcaatgac cgtttttacc gcccactctg ccaccgagcc tttatcaacc tcggcaaaca
    39781 attcgcgacg cagccgggtg tccgaaagca ggtgcgagta aagatcgaaa atagccgccg
    39841 tttctttttg tgctccggcg gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt
    39901 cttccagcgc cccggtcagt cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat
    39961 acacctgttc cattaaaggt aacgtggcat cctgccagcc ttcggcaatc gccacaccag
    40021 gtgctgccgg taatgcgcgg attcgcgtct ggcgatattg cccaaacagg gcagtcaact
    40081 gcgactgaga aagaatagct gccatctggg tggcaagcgt caccaggaag gattcttcac
    40141 tttcgtcata ctggcgcaac tctcgttgct gtaccaccag tacaccaagc aactggcgac
    40201 gttgaataat tggtacgcct aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt
    40261 atttgaagct ggggtgcttt tgcgcatctg caaggtttat cggttccgcc agcctgccaa
    40321 ccaggccgac gatcccttca tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca
    40381 gcccccgggt cgccatcagg tagtaacaac gtcgatcatg atcggccagg tagaccgaac
    40441 agacctcggt atccatcgca agacagatgt cggtaaccag aatatttaac gcctcattca
    40501 ggcgtggtgc gctggctacc ttttcgacta tttcgcgcag gcgagtgagc ataattggcg
    40561 tgacttaacc tcttttacgt cgataagcag atgcgttttg tggttttggc gtattttcct
    40621 gcagtgacat caccacactc gcgaactctt tcattaccct acggtagaca tcacgtttaa
    40681 atgacaccac ctgtctgacc ggataccagt aacttaccca tcgccagccg tcaaactctg
    40741 gtgtactgct ggtttgcata ttgatttctg catcgccgct taccagctgc aagagaaacc
    40801 atttttgttt ttggccgata caaaccggct tcgtgtccca acgcaccaaa cgtttcggta
    40861 atttgtagcg caaccagtta cgcgttgaag caaggattcg aacgtctttg cggcttaatc
    40921 ctacttcttc aaacaattca cggtacatcg cctgctctgc ggattctccg gggttgattc
    40981 cgccttgcgg aaattgccag gagtgctgac caaatcgccg ggcccacatt acctgcccct
    41041 ggcgattaca aatcacgata cctacgtttg ggcggtagcc atcgtcatca atcaccggac
    41101 tacctcaaaa taaagcttta tatacgaatg attgtttcat actccaggaa gacggtaaac
    41161 cactctctgc agggcattac acactaataa caattgaata actcacagtt atgtgcagag
    41221 ttataaacag aggaaggggt ggatagccgt ttttattcac cttttctgtg gatagagttg
    41281 tgaagaacta cggaattact acgggaaaac ccggagcatt ctgaataagc ccgatataga
    41341 cattttaaaa tattcattat tagtcaatgc attacatgta atttgagtaa tgaaaaagag
    41401 taattcgtga cccaggtcac acctctcatt tacgggttgg cgaaagatca accaatgccg
    41461 tgtttatcca cagaatgtgc cactaagtta agcactgaac cactaaaaac tggagtttcg
    41521 tcgcacgtca aggctgtaaa tgaaaacagt agtggaggtt tttcacagtt atcccagctt
    41581 tctgtggata acatggtgta agatcctgtt tattttcagt gaccagattt ggaaaaccta
    41641 ttgcagtgtt gcgcaactcg attaccggca gcctaaaaag caatataaat cagagaattg
    41701 aacaacacat gtggaaaaag ttacactgcg aatattcggc acataattgc tgtttgtttt
    41761 ttaaccaagg tatcatgaca tgtcccaacc tcgcccactg ctctctcctc ccgaaactga
    41821 agaacagttg ttagcgcaag cacagcaact ttctggttat acattgggag aactggcggc
    41881 acttgacggg ctggttacgc cagagaattt aaaacgcgat aagggctgga ttggcgtgtt
    41941 actggagatc tggctaggtg ccagcgcagg gagtaaacct gagcaagatt ttgctgctct
    42001 gggcgtggaa cttaaaacta tccctgtgga tagtcttggt cgtccgctgg aaacaacatt
    42061 cgtttgtgtt gcccggttaa cgggtaatag cggggtgacc tgggaaacca gccatgtgcg
    42121 ccacaagctc aaacgcgtac tgtggatacc ggttgaaggc gagcgcagca tcccgctggc
    42181 gcagcgtcgc gtaggatcac cgttgctgtg gagcccgaat gaagaggaag accgacagct
    42241 acgcgaagac tgggaagaat taatggatat gattgttctc ggtcaggttg agcggatcac
    42301 cgctcgtcac ggggaatatt tacagataag accgaaagca gcgaatgcga aagcgcttac
    42361 cgaagccatt ggtgcccggg gcgaacggat tctgacgctg ccacgcggct tttatttgaa
    42421 gaagaatttc accagtgcac tactggcccg tcattttctg atccagtagc catcgctttg
    42481 acctgccgct ttccgggcat ataattaccg cttcattttt ttggcagggc tttttagatg
    42541 ttatttgcat ggataaccga tcctaacgct tggcttgcgc tcggtacgct gacgctgctg
    42601 gagatcgttc ttgggatcga caatattatt ttcctttctc tggtggtggc aaagcttccc
    42661 acagcacaac gtgctcatgc gcgccgtctg gggttggcgg gagccatggt tatgcgtctg
    42721 gcgctgctgg catcaatctc ttgggttacg cgcctgacaa atccgctttt tacaatattc
    42781 agtcaggaaa tttctgcccg tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg
    42841 aaagccagca aggaaatcca cgaatccatt gaaggtgaag aagaagggct gaaaacacgc
    42901 gtttcatcat tcctcggcgc tatcgtgcag attatgctgc tggatattat cttcagcctc
    42961 gactcggtga ttaccgctgt gggtctgtca gatcacctgt ttattatgat ggcagccgtg
    43021 gtgattgccg taggcgtgat gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc
    43081 catccttcgg taaaaatgct ggcgctctct ttcctgattc tggtgggctt taccctgatt
    43141 ctggaaagtt tcgacatcca cgtaccgaaa ggttacatct acttcgcgat gttcttctct
    43201 attgcggttg aaagcctcaa cttgattcgc aacaaaaaga atccgctctg atactccgta
    43261 cgctctcctg cgggagggcg tactcactcc ttttaccctc acagtttaag attttcctgc
    43321 tttcaagata tataacgtcg gtttataaac agactattat cataggtagg ccaaatgagg
    43381 ataaccggat gaaaaaatgg gcagtaataa tttctgcagt cggactggcg tttgctgttt
    43441 ccgggtgttc cagtgattac gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg
    43501 gaaaacctga aattgatgat gataccgggc tggtgagtta tcacgatcag caaggtaacg
    43561 cgatgcaaat taaccgtgat gatgtttcgc aaattattga acgttaacaa ataaggtcag
    43621 catccggctg gccttaagat ttttctcttt cccttttccc ttccctctgc catttttata
    43681 ttccttatgt cgtgattata aaaaggaaac ggctatgcaa tatcaccgta taccccacag
    43741 ttcgctggaa gtcagcacgc tggggcttgg cacgatgacg tttggtgaac agaacagcga
    43801 agccgacgcc cacgcacaac tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt
    43861 tgccgaaatg tacccagtac ctccgcgccc cgaaacgcaa gggttaaccg aaacctacgt
    43921 cggcaactgg ctggcgaaac atggcagccg cgaaaagtta attatcgcct ccaaagtgag
    43981 cggaccgtcg cgcaataatg acaagggcat ccgcccggat caggcgctgg atcggaagaa
    44041 tatccgcgaa gcgctgcatg acagcctcaa gcgcctacag actgattacc tcgatcttta
    44101 tcaggtgcac tggccgcagc gcccgaccaa ctgcttcggc aaactcggtt atagctggac
    44161 ggattctgcg cctgcggttt cgctgctgga tacgctggac gcactggcag agtaccaacg
    44221 cgcgggaaaa attcgttata tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta
    44281 cctgcatctg gcggacaaac acgatctgcc gcgtattgtc accattcaga acccttacag
    44341 tctgttaaac cgcagttttg aagtaggtct ggcagaagtc agccagtatg aaggggtcga
    44401 actgctggcc tattcgtgcc tgggtttcgg cacgctgacc gggaaatatc tcaatggtgc
    44461 aaaacccgct ggcgcacgta atacgctctt tagtcggttc acccgctata gcggtgagca
    44521 aacgcaaaaa gccgtcgcgg cgtatgttga tatcgccaga cgtcatggcc ttgatcctgc
    44581 tcagatggcg ctcgcgtttg tacgccgtca accgtttgtt gccagcactc tgctgggcgc
    44641 aaccacgatg gatcagctga aaactaacat cgaaagtttg catctggagt taagcgaaga
    44701 cgtattagct gaaattgaag cggtacatca ggtttatact tatcctgcac cataaagatt
    44761 aacgccggtt aaaaccggcg ttaaatatta atgacggcgc tgccagatcc acagcgccgt
    44821 tattgccagc gcaaatagcg caccgaagcc aatgccaatg ggcacgaccg ggatgcctac
    44881 cattaccgcc agcgagtaaa tgcccagcat caacaacatg gcgctgtttt cgccaaggtt
    44941 ttgtactgca atcgcattcc ccgccccgac gcttttttta ccccgctcct gtagcaacgc
    45001 attgagcgga acgacaaaaa agccccccag cacgccaatc agcatcaaca aggcataggc
    45061 tggcagcagc tcgtgttgca gggagaaaat cggtaccacc acgccaatca aaatcccggc
    45121 tggcatacag cgtgacacgg tttccagcgt aaccagcttc gctgccgcac ctgcgccaac
    45181 cacgatacca atcgctacca tcgcgttgag ataggtcggc gtagcgttat cggtaatgcc
    45241 cagcgccacc ggtacccaca gcaccaacag gaaacgcagc gtgacacccg ctccccagaa
    45301 taaactggtg cccaccagcg aaaaacgcgt ttcaccattg caccatagcg aggtgcaggc
    45361 attcaggaaa ctgcgggtca tgttgatgag attccaggac tgccccggac gcgccgccag
    45421 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    45481 agcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gtaaaatcgc
    45541 cgctatggca gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    45601 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    45661 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    45721 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    45781 cgccaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    45841 atagaactgc gctttcagta acgccagagt ggcaaacagt agcgcgttat cgccaaacgc
    45901 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtattagt
    45961 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    46021 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
    46081 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    46141 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    46201 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    46261 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    46321 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    46381 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    46441 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    46501 ggcgtgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    46561 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    46621 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    46681 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    46741 tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac caagtccggc acaatgcggt
    46801 aatgcagcgg gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc
    46861 ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg
    46921 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
    46981 cgacgccttt tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
    47041 gctgctgttt tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt
    47101 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    47161 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    47221 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
    47281 ttgcgggaat gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    47341 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    47401 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    47461 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt
    47521 cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    47581 ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa
    47641 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    47701 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    47761 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
    47821 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
    47881 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    47941 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    48001 aaatgccatc aataaaagag acgtgattag gcgtaattag tacgcgctcg cccttcagtg
    48061 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    48121 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    48181 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    48241 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    48301 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    48361 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    48421 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaaca cgaagttgac
    48481 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    48541 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
    48601 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccacccc ttgaaccaac
    48661 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
    48721 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
    48781 taataattca cccaaagcca gcgaagcttc ccggctggct gtgcatagtg caatggagtc
    48841 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
    48901 cggtctgatc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
    48961 acaggtggct tatcacaccg gtaatttctt attgattggc aacggttacc acaacgaaca
    49021 aaaagagcgt caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
    49081 tgccaaaatg atcccagatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
    49141 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctcttg acgatcgtta
    49201 cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
    49261 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
    49321 tgctgaaagt ggtattccgg ccaatgatcg gctggtgaca tttggcgaac cagacgaaag
    49381 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
    49441 ctgttataac gattcaatgg cggcgggcgc gatgggcgtt ctcaatgata atggtattga
    49501 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
    49561 tccgcgcctg accaccgtgc gttacccaat cgtaacgatg gcgacccagg ctgccgaact
    49621 ggctttggca ctggcggata atcgccctct cccggaaatt actaatgtat ttagtccgac
    49681 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
    49741 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
    49801 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt
    49861 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacctg
    49921 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
    49981 cgcccgattc acataacggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
    50041 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
    50101 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
    50161 tccgcacctg agtaattaat acgccagact gcgctaccag gaagcgaccc ggttcaattt
    50221 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
    50281 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
    50341 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat aacctgacgc accatagcac
    50401 cacacacctg ctccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
    50461 ctagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
    50521 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
    50581 ccggattaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagcg
    50641 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
    50701 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
    50761 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
    50821 gcattaagcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacttcaa
    50881 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
    50941 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
    51001 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
    51061 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg
    51121 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
    51181 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
    51241 tcagccgcga actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac
    51301 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
    51361 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
    51421 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
    51481 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
    51541 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
    51601 ctacgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
    51661 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaattaca
    51721 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
    51781 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
    51841 gggcgggggt aggtatttcg gtggttaacc cgctcaccgc actagattat gcggcaagcg
    51901 gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
    51961 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtgg gcatttacaa gcggggttac
    52021 cgaaactggt cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac
    52081 agcatcctcg gcatggatcg ccgcggtatc aaacacaggc aaaacactgc gctcttctgg
    52141 caccagtaaa ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag
    52201 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
    52261 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
    52321 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
    52381 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
    52441 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
    52501 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
    52561 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
    52621 atcgacgcta tgtagcagca tttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
    52681 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
    52741 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
    52801 gacaaacgcc tcaacggcca ggtgcccaat ctctattaac gaaaaaaggg ccggatgtac
    52861 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
    52921 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtaata
    52981 cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
    53041 tcaagcagtg tcaggaaggc cgcgccgata atcatattcg acacccagtt tgtcgtcgtc
    53101 gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
    53161 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
    53221 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
    53281 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct
    53341 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
    53401 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgcg
    53461 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
    53521 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ctgttttaac
    53581 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    53641 atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta
    53701 tttggcagga agaccaccag aataatcagc agaactgctg gtaaagcaag aacccccaac
    53761 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    53821 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    53881 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    53941 acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    54001 acaaacagga tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa
    54061 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    54121 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    54181 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    54241 cgccgcgtat cacgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    54301 ttttcctgcc agcagagagt aagacattgt gaaaaaatac gtgaacaatt cacacaggtg
    54361 tcaggtcgga aacagcataa atatggatta aattgctgcg acatgccgtt atgtgatgga
    54421 tattccaatt ttaaaattaa gttgaattat tgagattatt attaacctcc taattttaaa
    54481 gcagataaaa ttcataaagt tcattaattg ataattgata tggattattt cataaccatg
    54541 atatggatta tgatgatcta gagatataaa aaaccctgcc atgcggcagg gtcataaaag
    54601 taagaggaat gaattaacgc gccagccaac cgccatccac agcaatggta taaccattta
    54661 cataatctga agcgctggag gcaagaaaca ctaccggccc catcaggtca ctcggcagtc
    54721 cccaacggcc agctggaatg cgatcgagaa tttccgcgct acgttgttca tctgcccgca
    54781 gttgttgggt attgttggtc gccatgtaac ccggagctat cgcattaaca ttaatgttgt
    54841 gtttagccca ttcgtttgcc atcaatcgcg tcacacccat cacgccgctt tttgatgcgg
    54901 tataagaagg cacacggatc ccgccctgga aggagagcat tgacgcgata ttgataatct
    54961 tgccgccatt gccttgcgcg ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc
    55021 tcttgatatt caggttcatg acatcgtccc agtctttttc gctgaactcg agagcgtctt
    55081 cgcggcgaat caatccggcg ttattcacca ggatatcaat atgaccaaat tcagctaccg
    55141 cgcgatccag cagtccagga atgccatcaa tctttcgcag atcggcggtc aggcttaaaa
    55201 aacgacgccc cagcgccgtg acctgctcga tggtttcagt cggttcaacg atgttaatgc
    55261 caacaatgtc acagcccgct tgcgccagcc ccaacgccat cccctggccc agcccagtat
    55321 cacaaccagt gacgaccgca actttacctt ggagagaaaa tgcacttaaa atcataacaa
    55381 caccttattc gtttatgccc acaactagcg caaatcttta acgcccacat ggtccatatc
    55441 attaaagacc tggttttcac cgaccatgcc ccagataaag gtataagctt tggttccgac
    55501 gccggaatgg atcgaccagc tcggggagat caccgcctgc tcgttatgca tcacaatgtg
    55561 acgcgtttct tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa
    55621 atagaaataa acttccatcc ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt
    55681 acctggagcc agctccgtca gccccatact caattgacag gtttccagta catccgggac
    55741 aaaatatttg ttaatcgtgc gacggttact ggtgaggtta tcgcctaacg tgactggaga
    55801 tacttcgtcc ggtgtgactt ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta
    55861 ataaaacttc gccggagtgg cggtatcaat actggcaaag acaacttctt ttgcaccttt
    55921 accaacatac agggcgtcgc ggtgaccgat ttcatagcat tgaccatcga cagtaatcgt
    55981 accggcaccg ccaatattga taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc
    56041 gagttgttta ccaacttccc cgccaacgga aaccgtttta gttatcggca taatgccgcc
    56101 aacaataatt cggtcaatgt ggctgtaaac catggtgtac tcatcggcga caaatacctt
    56161 ttcaaccaaa aattcattgc gcagcccttg ggtatccagc gtttttgcgt gcgcaccgtg
    56221 gatgctctgt cttacgtcca catcaacctc cgataatcag tgaagcgtga aaatagaaaa
    56281 cgatagtgaa acactgtttc gttttctcta taaagggatg atatgcccat gggctagggt
    56341 tttcaattaa aaataaaaca atgttttatt tttaatttaa ggattaaaaa aagtgcagtg
    56401 tcgatcacga atatgtcatg agaatggaaa aatgagggag cccagaaaat gtatgtcgac
    56461 ttgccagcca tcattattca acagattaat gacggcagat atttatcggt aaggaggaaa
    56521 tgcagagcta ctcgtcacgt tcaatggtca atgccacgcc ctggcccccg ccgatacaaa
    56581 gcgttgccag ccctttgcgg gcattacgtt tcaccatttc atgaaccaga gaaaccagga
    56641 ttcggcaacc ggaagcgcct atcgggtgac cgagtgcgat cgcgccacca ttgacattga
    56701 cccgacgctc atcccactca agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt
    56761 cattagcctc gataagatcg acatcagcca actgccagcc tacacgctcc aggcaacggc
    56821 gggtcgcata caccggcgca attcccatca atgccggatc tacaccaacg ctggcaaatg
    56881 cgcggatgcg ggccagcacg ggtaaattca acgctcgtgc tttggcttcg ctcatcatca
    56941 ttaccgcagc tgcgccatcg tttatggatg atgcattccc cgcggtcaca gaaccgagac
    57001 tatcaaatga aggatttaaa cgggctaaac cttctgcact ggcgtcagtg cgtggctgtt
    57061 catcggtatc aacaaccaac gtctgaccgt tactttggat cattaccggg acgatctcat
    57121 ctttaaatcg tccggcgtca atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag
    57181 catcctgcaa ctgacggctg atgccatatt cgcgagccag attttcggcg gtgacaccaa
    57241 tatgataatc attgaaggca tcccacaacc catcatgcac cagactgtca accaactggc
    57301 tattgccaag ctgttcaccg gtgcggctat cagtcagaac atgtggtgcg cgactcatgt
    57361 tttcctggcc acctgcgatg acaatatcag cctcgccaca ctgtatcgcc tgagtagcca
    57421 gatgcagtgc tttaagaccg gaaccgcaaa cgtcattaat cgtgattgca gaaacgctgt
    57481 taggaagacc acctttaata gccgattgcc ttgccggatt ctgccctgcc cctgcagtca
    57541 acacctgacc aagaattact tcatccaccg catatgcagg aacgccggta cgttctatta
    57601 acgctttcac gaccagacta ccaagttcca cggcggaatg acccgctaac gcaccacgaa
    57661 agcagccgat aggtgtccgt aacgccccga caatcacaac gtctttcatc acaacctcgc
    57721 accaattaac agtgcaatag taaatgattg ttaattacag ttatcttaat tgtttaaaaa
    57781 aagtgatttt tatcacaaag gaaatatgcc tgagcagtag tcagagtcat aactggcacg
    57841 taaggtttgc aaccactaac ccaccaatag atgggtagat agggcgttaa tctcccatac
    57901 ttaacttggt ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg atacgttcag
    57961 ataatgtcta atatttggtc taaagaagaa actctgtgaa gtttcgcgct ctatggcaca
    58021 gccgttgatg caggcacgct attccttcct attcagttag gttcggcagg ggctgtggtc
    58081 ctgtttatta ctgctctggt cgcctggcct ttaacatatt ggccacataa agccttatgc
    58141 cagttcatcc tctcatcaaa aacatcagca ggtgaaggga taacgggcgc ggtaacacac
    58201 tactatggca agaagattgg taatctgatt accacgctgt acttcatcgc cttttttgtc
    58261 gtcgtgttga tatatgcagt ggcaattacc aactcactta cggaacagct ggcaaagcat
    58321 atggttattg atcttcgcat ccgtatgttg gtgagtctgg gtgttgtatt aattctgaat
    58381 ctcatttttc tgatgggacg tcatgccact attcgggtaa tgggattttt ggtattccca
    58441 ttgattgcct atttcttatt tctttccatt taccttgtcg gtagttggca acctgatcta
    58501 ttaacaaccc aggtagagtt caatcagaat acccttcacc agatatggat atcgattccc
    58561 gtgatggttt tcgcctttag ccatacgccc attatttcta cgtttgccat agacagacgt
    58621 gaaaaatatg gcgaacacgc tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc
    58681 atcatctgca taagtgtact gttctttgtc tttagctgcc tgctttctat tccatcttcg
    58741 tatattgaag cggctaaaga agaaggggtc accattttat cggcgctttc tatgctgccg
    58801 aacgccccag catggttgtc aatttccggg attattgtcg cagtagttgc gatgtcgaaa
    58861 tcattcctgg gtacgtactt tggcgttatt gaaggtgcca cagaggtcgt caaaacaaca
    58921 ctacagcagg ttggtgtaaa gaaaagtcgt gcatttaacc acgcactatc aattatgttg
    58981 gtatcgctga ttaccttcat tgtttgttgc attaacccga acgcgatttc gatgatttac
    59041 gcgatcagcg gcccgctcat tgccatgata cttttcatca tgcctacgct gtcaacgtat
    59101 ctcatcccgg cgcttaaacc ctggcgttcc atcggaaatc tgattacgct gatcgtgggt
    59161 atcctgtgcg tatcggtaat gttctttagc taatatccgt tgtggatggt tcaaaattgc
    59221 tacaattttg aaccattcca tttttcataa tattcatttg ttattattcc gccctatcac
    59281 ttctctcgtc atatcctaga attatcagtt agttaaacac acaaagagat ctgaacatct
    59341 taaataagac tatttaagat gcataactta gattcgcaag atatcttctg gtcattcaaa
    59401 aacaatttgc gatatagata ttctcatacc atgagatagt tatctggacc acgttcgacc
    59461 atgcaagatt aaatcagtga aacataatca tatttaataa cgagaaatgc atttttaaat
    59521 atcttaatca gaattatagc aaatacagat taatcgcaat ttttcccagc aaaattacaa
    59581 aatcaaggtg tattgacatc atcaatcacc aatgaaatta tgcaattata tacggatagg
    59641 gaggttctta acatgg
//
LOCUS       DAJVQR010000014        58563 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVQR010000014 DAJVQR010000000
VERSION     DAJVQR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..1100)
                     /locus_tag="OQM52_000902"
     CDS             complement(<1..1100)
                     /locus_tag="OQM52_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCS1562854.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OQM52_000903"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OQM52_000903"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1562855.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OQM52_000904"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OQM52_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1562856.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OQM52_000905"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OQM52_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1562857.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OQM52_000906"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OQM52_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1562858.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OQM52_000907"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OQM52_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1562859.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OQM52_000908"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OQM52_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1562860.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OQM52_000909"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OQM52_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCS1562861.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OQM52_000910"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OQM52_000910"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS1562862.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OQM52_000911"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OQM52_000911"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCS1562863.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKHLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OQM52_000912"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OQM52_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1562864.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OQM52_000913"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OQM52_000913"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS1562865.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="OQM52_000914"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="OQM52_000914"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1562866.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="OQM52_000915"
     CDS             11850..12014
                     /locus_tag="OQM52_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562867.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="OQM52_000916"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="OQM52_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1562868.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="OQM52_000917"
     CDS             13523..13768
                     /locus_tag="OQM52_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1562869.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="OQM52_000918"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="OQM52_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1562870.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="OQM52_000919"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="OQM52_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1562871.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="OQM52_000920"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="OQM52_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1562872.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="OQM52_000921"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="OQM52_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1562873.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="OQM52_000922"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="OQM52_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1562874.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="OQM52_000923"
     CDS             19431..19784
                     /locus_tag="OQM52_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1562875.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="OQM52_000924"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="OQM52_000924"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1562876.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="OQM52_000925"
     CDS             21084..22211
                     /locus_tag="OQM52_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1562877.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="OQM52_000926"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="OQM52_000926"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1562878.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="OQM52_000927"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="OQM52_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="OQM52_000928"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="OQM52_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26570..26743
                     /locus_tag="OQM52_000929"
     CDS             26570..26743
                     /locus_tag="OQM52_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562879.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="OQM52_000930"
     CDS             26833..27582
                     /locus_tag="OQM52_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1562880.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="OQM52_000931"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="OQM52_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1562881.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OQM52_000932"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OQM52_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1562882.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OQM52_000933"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OQM52_000933"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1562883.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OQM52_000934"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OQM52_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1562884.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OQM52_000935"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OQM52_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1562885.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OQM52_000936"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OQM52_000936"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1562886.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="OQM52_000937"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="OQM52_000937"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1562887.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OQM52_000938"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OQM52_000938"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS1562888.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /locus_tag="OQM52_000939"
     CDS             complement(35511..35606)
                     /locus_tag="OQM52_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562889.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /locus_tag="OQM52_000940"
     CDS             complement(35720..35887)
                     /locus_tag="OQM52_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562890.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36054)
                     /locus_tag="OQM52_000941"
     CDS             complement(35890..36054)
                     /locus_tag="OQM52_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1562891.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OQM52_000942"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OQM52_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1562892.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OQM52_000943"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OQM52_000943"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1562893.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="OQM52_000944"
     CDS             40510..40761
                     /locus_tag="OQM52_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1562894.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OQM52_000945"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OQM52_000945"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1562895.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="OQM52_000946"
     CDS             42066..42824
                     /locus_tag="OQM52_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1562896.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="OQM52_000947"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="OQM52_000947"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS1562897.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OQM52_000948"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OQM52_000948"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1562898.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="OQM52_000949"
     CDS             complement(44424..45044)
                     /locus_tag="OQM52_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1562899.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OQM52_000950"
     CDS             complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OQM52_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1562900.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="OQM52_000951"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="OQM52_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1562901.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="OQM52_000952"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="OQM52_000952"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1562902.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="OQM52_000953"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="OQM52_000953"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1562903.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="OQM52_000954"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="OQM52_000954"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1562904.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="OQM52_000955"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="OQM52_000955"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1562905.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="OQM52_000956"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="OQM52_000956"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1562906.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="OQM52_000957"
     CDS             53106..53285
                     /locus_tag="OQM52_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS1562907.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="OQM52_000958"
     CDS             53371..53871
                     /locus_tag="OQM52_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1562908.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="OQM52_000959"
     CDS             53917..54423
                     /locus_tag="OQM52_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1562909.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OQM52_000960"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OQM52_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1562910.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="OQM52_000961"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="OQM52_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="OQM52_000962"
     CDS             55478..56221
                     /locus_tag="OQM52_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1562911.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANIRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="OQM52_000963"
     CDS             56251..56790
                     /locus_tag="OQM52_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1562912.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="OQM52_000964"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="OQM52_000964"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1562913.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OQM52_000965"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OQM52_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1562914.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58276..>58563
                     /locus_tag="OQM52_000966"
     CDS             58276..>58563
                     /locus_tag="OQM52_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1562915.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKK"
BASE COUNT        14060 a        15144 c        14641 g        14718 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag gtgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct ggcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaactcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggatttttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aatcctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatacg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa
    58081 ctaggcctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    58141 cagtgaaaat gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac
    58201 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    58261 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    58321 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    58381 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    58441 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    58501 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
    58561 aaa
//
LOCUS       DAJVQR010000015        57818 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVQR010000015 DAJVQR010000000
VERSION     DAJVQR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..65)
                     /locus_tag="OQM52_000967"
     CDS             complement(<1..65)
                     /locus_tag="OQM52_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1562916.1"
                     /translation="MECRPGCGACCTAPSISSPIPG"
     gene            220..792
                     /gene="yeiP"
                     /locus_tag="OQM52_000968"
     CDS             220..792
                     /gene="yeiP"
                     /locus_tag="OQM52_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1562917.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1015..2481
                     /locus_tag="OQM52_000969"
     CDS             1015..2481
                     /locus_tag="OQM52_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1562918.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2599..3585
                     /gene="yeiR"
                     /locus_tag="OQM52_000970"
     CDS             2599..3585
                     /gene="yeiR"
                     /locus_tag="OQM52_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1562919.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3624..4337
                     /gene="lpxT"
                     /locus_tag="OQM52_000971"
     CDS             3624..4337
                     /gene="lpxT"
                     /locus_tag="OQM52_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1562920.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4749..5315
                     /gene="mepS"
                     /locus_tag="OQM52_000972"
     CDS             4749..5315
                     /gene="mepS"
                     /locus_tag="OQM52_000972"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1562921.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5496..7052
                     /locus_tag="OQM52_000973"
     CDS             5496..7052
                     /locus_tag="OQM52_000973"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1562922.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7134..8948
                     /locus_tag="OQM52_000974"
     CDS             7134..8948
                     /locus_tag="OQM52_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1562923.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8949..10043
                     /locus_tag="OQM52_000975"
     CDS             8949..10043
                     /locus_tag="OQM52_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1562924.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10043..11068
                     /locus_tag="OQM52_000976"
     CDS             10043..11068
                     /locus_tag="OQM52_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1562925.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11070..12659
                     /gene="yejF"
                     /locus_tag="OQM52_000977"
     CDS             11070..12659
                     /gene="yejF"
                     /locus_tag="OQM52_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1562926.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12663..13007)
                     /locus_tag="OQM52_000978"
     CDS             complement(12663..13007)
                     /locus_tag="OQM52_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1562927.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13340..14530)
                     /gene="bcr"
                     /locus_tag="OQM52_000979"
     CDS             complement(13340..14530)
                     /gene="bcr"
                     /locus_tag="OQM52_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCS1562928.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14558..15253)
                     /gene="rsuA"
                     /locus_tag="OQM52_000980"
     CDS             complement(14558..15253)
                     /gene="rsuA"
                     /locus_tag="OQM52_000980"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1562929.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15403..17163
                     /locus_tag="OQM52_000981"
     CDS             15403..17163
                     /locus_tag="OQM52_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1562930.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17288..17572
                     /gene="rplY"
                     /locus_tag="OQM52_000982"
     CDS             17288..17572
                     /gene="rplY"
                     /locus_tag="OQM52_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1562931.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17711..18718)
                     /gene="yejK"
                     /locus_tag="OQM52_000983"
     CDS             complement(17711..18718)
                     /gene="yejK"
                     /locus_tag="OQM52_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1562932.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18900..19127
                     /locus_tag="OQM52_000984"
     CDS             18900..19127
                     /locus_tag="OQM52_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1562933.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19147..20907
                     /gene="pbgA"
                     /locus_tag="OQM52_000985"
     CDS             19147..20907
                     /gene="pbgA"
                     /locus_tag="OQM52_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1562934.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            20982..21058
                     /locus_tag="OQM52_000986"
     tRNA            20982..21058
                     /locus_tag="OQM52_000986"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21016..21018,aa:Pro,seq:ggg)
     gene            complement(21161..23752)
                     /gene="yejO"
                     /locus_tag="OQM52_000987"
     CDS             complement(21161..23752)
                     /gene="yejO"
                     /locus_tag="OQM52_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1562935.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24072..24719
                     /gene="narP"
                     /locus_tag="OQM52_000988"
     CDS             24072..24719
                     /gene="narP"
                     /locus_tag="OQM52_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1562936.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24930..25982)
                     /gene="ccmH"
                     /locus_tag="OQM52_000989"
     CDS             complement(24930..25982)
                     /gene="ccmH"
                     /locus_tag="OQM52_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1562937.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(25979..26536)
                     /gene="dsbE"
                     /locus_tag="OQM52_000990"
     CDS             complement(25979..26536)
                     /gene="dsbE"
                     /locus_tag="OQM52_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1562938.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26533..28476)
                     /gene="ccmF"
                     /locus_tag="OQM52_000991"
     CDS             complement(26533..28476)
                     /gene="ccmF"
                     /locus_tag="OQM52_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1562939.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28473..28952)
                     /gene="ccmE"
                     /locus_tag="OQM52_000992"
     CDS             complement(28473..28952)
                     /gene="ccmE"
                     /locus_tag="OQM52_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1562940.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(28949..29158)
                     /gene="ccmD"
                     /locus_tag="OQM52_000993"
     CDS             complement(28949..29158)
                     /gene="ccmD"
                     /locus_tag="OQM52_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1562941.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29155..29892)
                     /gene="ccmC"
                     /locus_tag="OQM52_000994"
     CDS             complement(29155..29892)
                     /gene="ccmC"
                     /locus_tag="OQM52_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1562942.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29934..30596)
                     /gene="ccmB"
                     /locus_tag="OQM52_000995"
     CDS             complement(29934..30596)
                     /gene="ccmB"
                     /locus_tag="OQM52_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1562943.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30593..31210)
                     /gene="ccmA"
                     /locus_tag="OQM52_000996"
     CDS             complement(30593..31210)
                     /gene="ccmA"
                     /locus_tag="OQM52_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1562944.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31229..31831)
                     /gene="napC"
                     /locus_tag="OQM52_000997"
     CDS             complement(31229..31831)
                     /gene="napC"
                     /locus_tag="OQM52_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1562945.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31841..32290)
                     /gene="napB"
                     /locus_tag="OQM52_000998"
     CDS             complement(31841..32290)
                     /gene="napB"
                     /locus_tag="OQM52_000998"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1562946.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32287..33150)
                     /gene="napH"
                     /locus_tag="OQM52_000999"
     CDS             complement(32287..33150)
                     /gene="napH"
                     /locus_tag="OQM52_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1562947.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33137..33832)
                     /gene="napG"
                     /locus_tag="OQM52_001000"
     CDS             complement(33137..33832)
                     /gene="napG"
                     /locus_tag="OQM52_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1562948.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33839..36325)
                     /gene="napA"
                     /locus_tag="OQM52_001001"
     CDS             complement(33839..36325)
                     /gene="napA"
                     /locus_tag="OQM52_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1562949.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36322..36585)
                     /gene="napD"
                     /locus_tag="OQM52_001002"
     CDS             complement(36322..36585)
                     /gene="napD"
                     /locus_tag="OQM52_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1562950.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36575..37069)
                     /gene="napF"
                     /locus_tag="OQM52_001003"
     CDS             complement(36575..37069)
                     /gene="napF"
                     /locus_tag="OQM52_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1562951.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37477..37965
                     /gene="eco"
                     /locus_tag="OQM52_001004"
     CDS             37477..37965
                     /gene="eco"
                     /locus_tag="OQM52_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1562952.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38087..39118)
                     /locus_tag="OQM52_001005"
     CDS             complement(38087..39118)
                     /locus_tag="OQM52_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCS1562953.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39352..39711)
                     /locus_tag="OQM52_001006"
                     /pseudo
     CDS             complement(39352..39711)
                     /locus_tag="OQM52_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39929..41572)
                     /gene="yojI"
                     /locus_tag="OQM52_001007"
     CDS             complement(39929..41572)
                     /gene="yojI"
                     /locus_tag="OQM52_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1562954.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41648..42298)
                     /gene="alkB"
                     /locus_tag="OQM52_001008"
     CDS             complement(41648..42298)
                     /gene="alkB"
                     /locus_tag="OQM52_001008"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1562955.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42298..43362)
                     /gene="ada"
                     /locus_tag="OQM52_001009"
     CDS             complement(42298..43362)
                     /gene="ada"
                     /locus_tag="OQM52_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1562956.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43436..44491)
                     /gene="apbE"
                     /locus_tag="OQM52_001010"
     CDS             complement(43436..44491)
                     /gene="apbE"
                     /locus_tag="OQM52_001010"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1562957.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44603..45724)
                     /gene="ompC"
                     /locus_tag="OQM52_001011"
     CDS             complement(44603..45724)
                     /gene="ompC"
                     /locus_tag="OQM52_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1562958.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46462..49134
                     /gene="rcsD"
                     /locus_tag="OQM52_001012"
     CDS             46462..49134
                     /gene="rcsD"
                     /locus_tag="OQM52_001012"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1562959.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49151..49801
                     /gene="rcsB"
                     /locus_tag="OQM52_001013"
     CDS             49151..49801
                     /gene="rcsB"
                     /locus_tag="OQM52_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1562960.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50001..52850)
                     /gene="rcsC"
                     /locus_tag="OQM52_001014"
     CDS             complement(50001..52850)
                     /gene="rcsC"
                     /locus_tag="OQM52_001014"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1562961.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53125..53901)
                     /locus_tag="OQM52_001015"
     CDS             complement(53125..53901)
                     /locus_tag="OQM52_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS1562962.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53906..55555)
                     /locus_tag="OQM52_001016"
     CDS             complement(53906..55555)
                     /locus_tag="OQM52_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1562963.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEEELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55556..56115)
                     /locus_tag="OQM52_001017"
                     /pseudo
     CDS             complement(55556..56115)
                     /locus_tag="OQM52_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56274..>57818)
                     /locus_tag="OQM52_001018"
     CDS             complement(56274..>57818)
                     /locus_tag="OQM52_001018"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1562964.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14150 a        15605 c        14196 g        13867 t
ORIGIN      
        1 ccgggaatag ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc cggacggcat
       61 tccatacgtt gttctcttaa ggttaaccac cggattgatg agcgcgcaca gtaccacctt
      121 ttttgcacca gcaaaagtgc gaataccact tgcccgaaag gcccgtcgcg agtactttgt
      181 cgcgatattt ttgacatttt cgactacagg aatttttcga tgccaagagc gaacgaaatt
      241 aaaaaaggta tggtactgaa ttacaacggc aaactgctgc tggctaagga tattgatatt
      301 cagtcgccca ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt
      361 accgggctga aagtagaaga gcgtttcaaa ggtgatgata tcgttgacac cgtgacgctg
      421 acccgccgtt acgttgactt ctcctatgtc gatggcaacg aatatgtctt tatggataaa
      481 gaagactata ccccgtatac cttcaccaaa gatcagattg aagaagagtt gctgtttatg
      541 ccagagggcg gcatgccgga catgcaggtg ctgacctggg acggtcaact gctggcgctt
      601 gagctgccgc agaccgttga tctggaaatc gttgaaaccg caccaggcat caaaggggca
      661 tctgccagcg cccgtaacaa accggcgaca ttgagcactg gtctggtgat tcaggtacca
      721 gaatacttaa gcccgggcga aaaaattcgt atccatatcg aagaacgccg ttatatgggg
      781 cgtgctgact aacttcagcc gcatgcagaa aagggatagc tcaggctgtc ccttttttaa
      841 tttattatac caatcttcta ttttgcgctt cctgaacacc ttacgccctc aattttcact
      901 cgttgattga tcgccctcac acttcatcgc attaacaatc cagaccaatt tcaattgctg
      961 tcatataact ttacactgtc attgttaatt tatcgttact aagacgtgac tcctatgaag
     1021 acaattgcct ccgttacgct cccgcatcat gtacacgctc cacgctacga tcgccagcag
     1081 ttgcaatcac gtatcgttca ttttggcttt ggcgcctttc accgcgctca tcaggcgtta
     1141 ctgaccgatc gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga aatcagcttg
     1201 ttcagcggtg atcaactgat gagccagctc cgcgcacaga accatttata taccgtgctg
     1261 gagaaaggtg cggacggcaa tcaggtgata attgtcggtg ccgttcacga atgccttaat
     1321 gcaaaactgg attccttagc ggcaattatt gagaaatttt gcgagccaca ggtggcaatt
     1381 gtttccctga cgattaccga aaaaggctat tgtattgacc cggccaccgg tgcactcgac
     1441 accagtaatc cgcggattat tcacgatcta caaacccctg aagaacctca ctccgcaccg
     1501 ggtattctcg tcgaagcact gaaacgccgc cgtgagcgca gccttacacc gtttaccgtg
     1561 ctctcctgcg acaatattcc cgacaatggc catgtggtga aaaacgcggt gctgagaatg
     1621 gcagaaaaac gttcgccaga actcgccggg tggataaaag aacacgtcag ttttccggga
     1681 accatggtcg accgcattgt tccggctgca accgacgaat cactggcaga aatcagccag
     1741 catctggggg taaatgatcc ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtt
     1801 gaagataact tcatcgctgg gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat
     1861 gatgtcctgc catgggaaga gatgaaactg cgtatgctta atggcagcca ctcttttctc
     1921 gcttatctgg gttacctctc aggattcgcc tatatcagtg attgcatgca ggatcgcgta
     1981 tttcgctatg ctgccagaac attaatgctg gatgagcaag cgccgacact gcaaattaaa
     2041 gatgtcgatt taacacaata tgcggataag ttaattgcac gttttgctaa tccgtcgctg
     2101 aaacataaga cctggcaaat cgcgatggat ggcagccaga aattaccgca acgtatgctg
     2161 gcaggtattc gcatacatct ggggcgtgaa acggactggt cgttgctggc attaggcgtt
     2221 gctggctgga tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc
     2281 gatccgctta gcgataaaat tcgcgaactt gttgcgggca gcagcagtga acaacgcgta
     2341 accgccctgc tttccctgcg tgaagttttc ggtgatgatc tgccagataa cccgcatttt
     2401 gtgcaggcca tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta
     2461 ttaaacaccc tcaaaattta acgatttctg cggttaaagc ggatgaagct caccttcgtc
     2521 cgctctcccc ttctcttttc tgcctttttt agccaggatt aacgctcagt taacttacca
     2581 gagtattgcg gagccgttgt gaccaggacc aacctcatca ccggttttct cggcagcggg
     2641 aaaaccacgt cgattcttca tctgttagcc cataaagatc ccaacgaaaa atgggcggta
     2701 ctggttaatg aatttgggga agtcggaatt gatggtgctt tgctcgccga tagcggcgca
     2761 ttgctgaaag agatccccgg cggctgcatg tgctgcgtta atggtttacc catgcaggta
     2821 gggttgaata ccttactgcg tcagggaaaa ccagaccgct tgttgataga gccgaccggg
     2881 ctgggccatc cgaaacagat cctcgatctg ttaaccgcac cagtctatga accgtggata
     2941 gatctgcgcg ccaccttgtg cattctcgat ccacgcctgc tgctggacga aaaaagcgcc
     3001 agcaatgaaa acttccgtga ccagctggct gccgcagaca tcattgtcgc caataaatcc
     3061 gaccgtgcga cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc
     3121 gatcgacaat taattcacag tgagcatggg aaagttgacg gtcatcttct ggatttgccg
     3181 cgtcgcaatt tagccgagtt gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa
     3241 aaagggttag cagcgttaag cctgccagag catcaacgct ggcgtcgcag tctgaacagc
     3301 gggcaaggat atcaggcctg cggctggata ttcgacgctg atacggtatt cgacaccatt
     3361 ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt
     3421 cccgaagggc tggtacgaat caaccgtcag ggcgatgacc tgcacattga aacgcaaaac
     3481 gttgcgccac cggacagccg tattgagctg atttccagca gcgaagctga ctggaatgcc
     3541 ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt
     3601 tcagtaagat aattagagaa aatatgatta aaaatttgcc gcaaatagtg ttgttgaata
     3661 ttgtcggccc cgcgctgttt ctttcctggt atattcccgt taatcatgga ttctggttgc
     3721 cgattgatgc ggatattttt tatttcttta atcagaaact ggtcgaaagt aaggcctttt
     3781 tatggctggt tgcattgact aacaatcgcg ccttcgacgg ttgttcactg ctggcgatgg
     3841 gtatgttgat gctgagtttc tggctgaaag aaaacgcccc tggcagacga cgtatcgtga
     3901 ttatgggtct ggtcatgcta ttaactgcag tggtattaaa ccagctgggt caggcattaa
     3961 ttcctgtaaa acgggccagc ccaacattga cttttaccga tattaaccgc gtcagcgaac
     4021 tgctctctgt tcccacgaaa gatgcctcac gagatagctt tcccggcgat cacggcatga
     4081 tgctgcttat tttttcggca ttcatgtggc gttatttcgg caaagttgca ggccttatcg
     4141 cccttattat ttttatggtt tttgcatttc ccagagtaat gattggcgca cactggttta
     4201 ctgacatcat tgtcggttcg atgaccgtga tattgatcgg tttgccctgg gtgttgctga
     4261 cgccattaag tgatcgatta atcacctttt ttgacaaatc actaccagga aaaaacaaac
     4321 atttccaaaa caaataactc acagtaatta acatcatcag ggttattttt atagtgagga
     4381 taatcctgat gatgcgcacc gtgctttcat ctatcgaacg caaaaatcat tctctaagta
     4441 aatgaatgga ttgcatgcgt ttcactcaat tgtactttaa ttgaccaacc ccgcttatta
     4501 actttctgta tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc
     4561 aatcgggtaa tctcgaactc gttttgcctc ggcggtagat tatcctcaca gcatataatt
     4621 ttgtgcgtta gtccacagat ttggccttaa ggaattgttt caacatgccc aggtaattag
     4681 tctcgtgtcg cttggcattt ttttataacg atatttgtcg ttaaggactt caagggaaaa
     4741 caaacaacat ggtcaaatct caaccgattt tgagatatat cttgcgcggg attcccgcga
     4801 ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa caccgcaaag aatatgcatc
     4861 ctgagacacg tgcagtgggt agtgaaacat catcactgca agcttctcag gatgaatttg
     4921 aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat gctgactgga
     4981 aaggcgtacg ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt tctggtttcg
     5041 tacagcgtac attccgtgag caatttggct tagaacttcc gcgttcgact tacgaacagc
     5101 aggaaatggg taaatctgtt tcccgcagta atttgcgtac gggtgattta gttctgttcc
     5161 gtgccggttc aacgggacgc catgtcggta tttatatcgg caacaatcag tttgtccatg
     5221 cttccaccag cagtggtgtt attatttcca gcatgaatga accgtactgg aagaagcgtt
     5281 acaacgaagc acgccgggtt ctcagccgca gctaataaac cgtttggatg caatcccttg
     5341 gctatcctga cgagttaact gaaagcactg cttaggcagt gcttttttgt tttcattcat
     5401 cagagaaaat gatgtttccg cgtcttgatc caggctatag tccggtcatt gttatctttt
     5461 aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt catacgcgct cccaattctg
     5521 gacgtaagct cctgcttacc tgcattgtag caggcgtgat gattgcgata ctggttagct
     5581 gccttcagtt tttagtggcc tggcataagc acgaagtcaa atacgacaca ctgattatcg
     5641 acgtacaaaa gtatctcgat acctattttg ccgacctgaa atccactact gaccggcttc
     5701 agccgctgac cttagatacc tgccagcagg ctaaccccga actgaccgct cgcgcggcgt
     5761 ttagcatgaa tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc tgttcatctg
     5821 cgactggtga gatggacatt ccactcaatg aattgattcc ggcgctcgac attaataaaa
     5881 acgtcgatat ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca
     5941 tctggtatcg caaccctttg ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc
     6001 tgacgccttc tctcttttat agttcacggc aggaagatta cgatggcgtc gccctcatta
     6061 ttggcaatac tgcgctatct accttttctt cacgtttgat gaacgttaat gaattaaccg
     6121 acatgccggt ccatgaaact aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg
     6181 atgactggac atggaacgat gtgtggtacg catttttact gggcggcatg agtggaactg
     6241 tcgttggcct gctttgctat tacctgatga gcgtacgtat gcgccccgac agagaaatca
     6301 tgaccgccat caagcgcgaa caattttacg tggcgtatca accggtggtg gatacacaag
     6361 ctttgcgggt aacgggcctg gaagtactgc tacgctggcg gcatcctgta gcgggagaaa
     6421 ttcccccgga tgccttcatt aactttgccg aatcgcaaaa gatgattgtg ccgctgactc
     6481 agcacctgtt tgagttaatt gcccgcgatg ccgcagaatt agaaaaagta ctgccggtag
     6541 gcgtcaaatt tggcattaac attgcgccgg accatctgca cagcgaaagc tttaaagcag
     6601 atatccagaa actgctcact tccctgcccg cacaccattt ccagattgtg ctggaaatta
     6661 ccgagcgcga tatgctgaaa gagcaagaag ccacacaact cttcgcctgg ctgcactcgg
     6721 tcggcgtaga aattgctatt gatgacttcg gcaccgggca cagcgcgctt atctatcttg
     6781 agcgttttac gctcgattat ctgaaaattg accgtggatt tatcaacgcc atcggtacgg
     6841 aaacgatcac ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgt ctcaatatgc
     6901 tgacagttac tgaaggggtc gaaacgccgg aacaagcgcg atggctaagc gaacgcggcg
     6961 ttaatttcat gcaaggctac tggattagtc acccgttacc gctggacgat tttgttcgct
     7021 ggctgaagaa accgtatacg ccgcagtggt aaggtgtgct tacgtccctt attattcata
     7081 gtgaaagcat gccggattgc ggctaatgat gagtaaaagg aaatccgttg cagatgattg
     7141 tgcgcatact gctgctgttt atcgctctgt tcaccttcgg tgcgcaggcg caggctatca
     7201 aggaaagcta tacctttgcc gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt
     7261 ttgattatgt gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca gccctcggta
     7321 ccttcgataa tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc accgagcagc
     7381 tgtacgacac gttatttacg acttccgatg acgaaccagg cagttattac ccgctgattg
     7441 ctgaaagtgc acgctatgct gacgattatt cctgggtgga ggtcgctatt aatccgcgcg
     7501 cccgttttca tgatggttct cccattactg cccgcgatgt agagtttact tttcaaaaat
     7561 ttctgaccga aggcgtgccg caatttcgtc tggtctacaa aggcaccacc gtcaaagcca
     7621 ttgcgccgtt aaccgtgcgc attgagttag ctaaacccgg caaagaagat atgttgagtc
     7681 tgttttcgct gccggtattt ccagaaaagt actggaagga tcacaaactt agcgacccgc
     7741 tcgccacgcc tccgcttgcc agtggtccgt accacgttac gtcctggaaa atggggcaaa
     7801 atattgtcta ttcccgcgtg aaagattact gggcagcaaa cttaccggta aaccgtggac
     7861 gctggaattt cgacaccatt cgctacgatt attacctcga tgataatgtc gcctttgaag
     7921 cgtttaaagc aggtgccttt gatttgcgta tggaaaacga cgctaaaaac tgggccacgc
     7981 gttataccgg taaaaatttc gataaaaaat acatcatcaa agatgagcaa aagaacgaat
     8041 cagcccagga tacgcgctgg ctggcgttta atatccaacg tgcggtattc agcgatcgcc
     8101 gggtgcggga agcaatcact ctcgcctttg actttgaatg gatgaacaag gcgttgtttt
     8161 acaacgcctg gagtcgcacg aacagttatt ttcaaaatac cgaatacgcg gccagaaatt
     8221 accccgacgc cgcggagctg gtgcttctgg caccaatgaa aaaagatcta ccgccagaag
     8281 tcttcacaca aatctaccag ccgccggtat ctaaaggcga tggctacgat cgtgacaacc
     8341 tgttaaaagc cgacaaactt ctcaacgaag cgggctgggt gctgaagggt cagcaacgcg
     8401 ttaatgccac aacgggtcag ccgctcagct ttgaattatt gcttcccgca agcagcaata
     8461 gtcagtgggt attgccgttc cagcacagcc tgcaacggct gggtatcaat atggacattc
     8521 gcaaggtgga taactcgcaa atcactaacc gcatgcgcaa tcgcgactat gacatgatgc
     8581 cgcgactatg gcgggcgatg ccgtggccca gttccgattt acagatttcc tggtcatcgg
     8641 aatatatcaa ttccacttat aatgcccccg gcgtgcaaag cccggttatc gactcactga
     8701 tcaaccaaat tattgccgcg cagggaaata aagaaaaatt actgccgttg gggcgagcac
     8761 tggatcgcgt attaacgtgg aattattaca tgctgccaat gtggtacatg gcggaagacc
     8821 gtctcgcctg gtgggataaa ttctcccaac ccgctgtacg ccctgtttac agcctgggta
     8881 tcgatacctg gtggtatgac gttaataaag cggccaaact gccgtcagcc agacaacagg
     8941 gagagtagat gggcgcttac ctgattcgcc gtctgttgct ggtgatccca acgttatggg
     9001 ctattatcac tatcaacttt ttcatcgtgc aaattgcgcc tggcggtccg gtcgatcagg
     9061 ccatcgccgc cattgagttt ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg
     9121 ttcgtgccag ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaat taccgtggcg
     9181 gacgcggatt agatccagaa gtgatcgctg agatcactca tcgctacggt ttcgataagc
     9241 cgatccacga acgttacttc aaaatgctct gggactacat ccgctttgat tttggtgata
     9301 gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga cagtctgccg gtttccatca
     9361 ccctcggatt gtggagcacg ctgattatct atctggtgtc gattccgtta ggcattcgca
     9421 aagctgttta taatgggagt cgctttgacg tctggagtag cgcatttatc atcatcggct
     9481 acgccattcc ggcctttttg tttgccatcc tgctgattgt cttcttcgcg ggcggcagct
     9541 atttcgacct gttccctcta cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt
     9601 atcagaaaat caccgattat ctgtggcata tcacgctacc ggtgctggcg acagtgattg
     9661 gtggctttgc ggcgctgacc atgctgacca aaaactcatt ccttgatgaa gtgcgcaagc
     9721 aatacgtggt gaccgcgcgt gcgaaagggg taagtgagaa aaacattctc tggaaacata
     9781 tgttccgcaa cgccatgctg ctggtgattg ccggttttcc ggcgacgttt atcagcatgt
     9841 tttttaccgg ctcgctgctg attgaggtga tgttttcact caatggtctt ggcttactgg
     9901 gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg tatattttca
     9961 ccctgattgg cctgctgctg aatattgtca gtgatatcag ctatacgctg gttgatccgc
    10021 gtatagattt tgagggacgt taatgtcgcg actcagcccc gtcaatcagg cccgttgggc
    10081 gcgttttcgt cataaccgtc gcggctactg gtcgttatgg attttcctcg ttttgtttgg
    10141 tttgagtttg tgttctgaac ttatcgctaa cgataaaccg ttgctggtgc gttatgacgg
    10201 cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg gcccgctggc
    10261 aagtcaggct gattatcagg acccgtggct gaaacaacgg ctggaaaata acggctgggt
    10321 actgtgggca ccgattcgct ttggtgctac cagtatcaac tttgctaccg ataagccctt
    10381 cccttctcca ccctcccggc aaaactggct gggaacggat gcaaacggcg gcgatgtgct
    10441 ggcacgcatt ctctatggca cgcggatctc ggttctgttt ggcctgatgc tgactctctg
    10501 ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc tattacggcg gtaaagtcga
    10561 tctctggggg caacgcttta ttgaagtatg gtcggggatg ccgacgctgt ttttgattat
    10621 tttactttcc agcgtcgtac agcctaactt ctggtggctg ctggcaatta ctgtcttgtt
    10681 tggctggatg agtctggtcg gcgtggtgcg ggcggagttt ttacgtactc gtaatttcga
    10741 ctacattcgt gcggcacagg cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat
    10801 gttgcctaat gccatggtcg cgaccctcac ctttttaccg tttattttat gtagttcgat
    10861 aaccaccctg acctcgctcg atttcctcgg cttcggtcta ccgctcggtt caccgtcact
    10921 cggtgaactg ctgttacaag ggaaaaataa ccttcaggcc ccgtggcttg ggatcaccgc
    10981 cttcttgtcg gtggcgatat tattgtcttt gctgatcttt attggtgaag ccgtccgcga
    11041 cgcatttgat cctaataagg cggtgtagca tgacgcaaac tctgttagcg attgaaaatt
    11101 tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt agtcaatgat gtttcactac
    11161 agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta
    11221 ccgcgctgtc aattttacgc ctgctccctt ccccgccggt tgaatatctc tccggcgata
    11281 ttcgttttca tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg
    11341 gtaataagat cgccatgatt tttcaggaac cgatggtgtc gttaaatcca ttgcataccc
    11401 tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc
    11461 gtggcgaaat tcttaactgc cttgatcgcg tcggtatccg ccaggcggca aaacggctga
    11521 cagattatcc gcatcagctc tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc
    11581 tgttaacgcg accggaatta ttaattgccg atgaaccgac caccgcactg gacgtctctg
    11641 tccaggcgca gattttacag ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc
    11701 tgtttattac tcataacctc agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc
    11761 aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct atttgcctca cccactcatc
    11821 cttacacaca aaagctactc aacagtgaac cgtcaggcga tccggtaccg ttgccagaac
    11881 ctgcctcaac gttgctggat gttgaacagc ttcaggttgc cttccccatt cgcaaaggga
    11941 ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa catcagtttt acgctacggg
    12001 cgggtgaaac actgggttta gtgggcgagt ctggttccgg gaaaagcacg acgggactgg
    12061 cgctgctgcg actgattacc tctcagggca gcatcgtctt tgacggtcag ccactgcaag
    12121 atttaaatcg ccgccagctg ttacctactc gtcatcgtat tcaggtggta tttcaggatc
    12181 caaactcctc gctcaaccca cgactcaacg ttttgcagat tattgaggaa ggcttacggg
    12241 ttcaccagcc gacgctttct gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg
    12301 aagtgggatt agatcctgaa acacgccacc gttatccggc ggagttctct ggtggtcagc
    12361 gacaacgtat tgcgattgcc agggcattaa ttcttaagcc ctcgctgatc atacttgatg
    12421 aaccgacatc atcactcgac aaaacggtac aggcgcaaat attgacgctt ttgaaatcat
    12481 tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca cgatttgcac gttgtccgcg
    12541 cgttatgtca tcaggttatc gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg
    12601 cgcgcgtgtt tgccacaccg cagcaggagt atacgcgtca gttactggcg ttgagctgac
    12661 gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa atttttcaat
    12721 cggcatgttg cagcaaactc gtcctggcga tttacaaaca ggcacggctc accttcacac
    12781 tccaacaccg aacatggaac ttcgatgtcg cttaacgcct ggcttagttt atacatcacc
    12841 gaccatgcat tatcaccatc cgggctaagc aatttaagcg ccaccaggct gttgtcaccg
    12901 cacggtccat tttgaggaat cggttcaacc ttcgaacctg cgaaaccagc agaccagcga
    12961 tagttctgcg gcagtcgatg gacgattgag agttgtaatg aagtcactta ttttccccgg
    13021 aagtacattc acttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct
    13081 ttatacagat cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc tgatttaagt
    13141 aatatctgcg ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact
    13201 caaccttttt aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac
    13261 atatatcggg gaaatgatag atttgtggtt gacatgcatc aacaaaggaa gccttttacc
    13321 ttcctcgttg tgcaatagat caccgttttt tcggccgact ggcgtacaga cagaagagaa
    13381 tggagctggt tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg
    13441 caagagaaag caatgcgcca acaattgccc ctatgccaaa acggaaggtt cctgccagcg
    13501 aagatgccgt tcccgccata tggggaaact catcaagaat gaccgccatc gcattggatg
    13561 acaccatcga cacgcagccc acaaacgccg caacgccaac caccagcgac cagaatccca
    13621 gccccagcgg cgcactgatg accatccacg cagccataat aaattgtatc cacaaccccg
    13681 agcggaacat atttaacgcg ccaatgcggc ggacgaagcg gctgttaaag atggtcatca
    13741 cgaacagaaa aacaatgttt agcgcaaagt aataaccaaa gttttccggc gcgacgtggt
    13801 taatttcaat ataaacaaac ggtccggcgc ttaagaatga gaacatcccg gcaaagctga
    13861 aaccactggc aagcatgtag ctcaggacac gtttatggcg gaacagcgcc gcaaagttac
    13921 caatagtggt acgaatgtga aatggctgac gacgctccgg tggtaaggtt tctttaatca
    13981 ggaagaaaat cattgccgaa gccagaatcg ccgctaatgc caggatccag aagatgtaat
    14041 gccagctcag ccacaccagc acccagccgc caactatcgg tgccatcagc agtgcaatgg
    14101 tcgtcaccag catgacaaac gacatcatcc gcgagaactc ttctttcggg taaatatcac
    14161 gcatcaaagc gttgataacc acgctggccg cagccgcagc cagcccgtgg aagaaacgca
    14221 tcacaatcag ctgatcgatg gtttgtgcca acgcacacgc caccgcggcg gcggcaaaca
    14281 ccagcgtccc gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga
    14341 ttaactgccc caacgcaaag cccagaatat aagtactgag ggtcatctgc gtactgcccg
    14401 ccgatacgcc aaactgcgct gaaattaccg gtagcgcggg cagatacata tcaatcgaca
    14461 gcggcatcaa catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc
    14521 gggtggtcac aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt
    14581 cttcagttaa cggacgatat tcaccggggg ctaaatcagc atccagcgta ataccgccaa
    14641 tacgttcacg atgcagctca accacgtggt tacccacggc ggcgaacatg cgtttcacct
    14701 gatgataacg cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttcaagca
    14761 ccgccggctt agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg
    14821 ccgtatcgtc agctacaggt gattccagtg tcaccagata ggtcttctcg caatgatggc
    14881 gcggagaagt aatgcggtgc gaccactgac catcatcagt catcagcacc agaccggtgg
    14941 tatcgatatc cagacgtcct gcggcgtgca gtttccacgc taccggttca tccagaaaat
    15001 agagcaccgt cgggtgatcc gggtcgtccg tggagcaaac atagccctga ggcttattga
    15061 gcatgaagta acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat
    15121 gttcagggag cagtttgaac gctgcattac gaacgatttc gccatcgacg gtgacacgat
    15181 tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt
    15241 tatcaagtcg catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa
    15301 aatctgaact gttgtctgcc cagtatagcg gtctaataac ttccctcaag ggaaaaagaa
    15361 ccgtggcata ctattagcag aataatctac ctacgcgaaa ccatgatttt tacactccgc
    15421 ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct
    15481 gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg tgatagcgga actggcacgg
    15541 ctggcgcgtg gtcgcgtact ggtgctggca cacgttaaag aactggtggc gcaaaaccat
    15601 gcaaagtatc aggcgctggg gctggaagcc gatatttttg ccgccgggct aaagcgcaaa
    15661 gagagccacg gtaaagtggt atttggcagc gttcagtcgg tcgcccgtaa tcttgatgcc
    15721 tttcagggtg aattttcgct gttgattgtc gatgaatgcc accgtattgg tgacgatgaa
    15781 gagagccagt atcagcaaat cctcactcac ctgactaaag tgaatcccca cttacgcctg
    15841 ctggggctga ctgccacgcc ttttcgattg ggcaaaggct ggatctacca gttccattat
    15901 cacggcatgg tacgcggcga tgagaaagcc cttttccgtg actgcattta tgagctgccg
    15961 ctgcgttata tgattaaaca cggctatctg acgccgccag aacgactgga tatgccagta
    16021 gtgcaatacg atttcagccg cttgcaggca cagagtaacg ggctgttcag cgaagctgat
    16081 ctcaaccgtg agctgaaaaa acagcaacgc attacgccac atatcatcag ccagatcatg
    16141 gagttcgccg aaaagcgcaa aggggtaatg attttcgccg ccaccgttga acacgcaaaa
    16201 gagattgtgg gattactgcc tgccgaagat gctgcactga ttactggcga caaccccggc
    16261 gctgagcgcg atgtgttaat tgaagatttt aaagcccagc gttttcgcta tctggtcaac
    16321 gtcgcagtac tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc
    16381 cctaccgaat cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg tctcgctccg
    16441 ggcaagactg attgtttaat tcttgattat gcgggtaatc ctcacgatct ctacgcgccg
    16501 gaagttggta caccgaaagg caaaagtgac aacgttccgg tacaggtttt ctgccctgcc
    16561 tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt gattgaacac
    16621 tttggtcgcc gctgtcaggg atggtttgaa gatgacgacg gtcatcgcga acagtgtgac
    16681 ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat tgccgctcgc
    16741 cgctgccgcg aatgtgacac ggtactggtt gatccggatg atatgttaaa agcggcgcta
    16801 cgactgaaag atgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac
    16861 gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggcgcgga tgtgagtgag
    16921 cgtttccgtc tgcaaacgcc cgcccagcgt accgccttcg agcagctttt tatccgcccg
    16981 catacgcgca caccgggcat ccctctgcgc tggatcaccg ccgccgatat cctcgcccag
    17041 caagccttac tgcgacatcc ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa
    17101 gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt
    17161 taatggtagt tttgattgat gtataaggcg attgagtata gaatctcgcc cgcttttgca
    17221 tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga
    17281 gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg
    17341 ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcaccgct
    17401 ggctatcgag ctggatcacg acaaagtcat gaacatgcaa gctaaagctg aattctacag
    17461 cgaagttcta accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca
    17521 gcgtcacccg tacaaaccga agctgcagca catcgacttc gttcgcgctt aattgctgaa
    17581 taagttgtaa aaaaccccgc tccggcgggg ttttttgtat ctgcagatta tgcctgatta
    17641 cggtattgct attttttgca ggccagataa ggcgttcacg ccgcatctgg cattcagaac
    17701 acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc aaattcggcg
    17761 gcgtgccttt aatggtcaag gtatcggttg ctggatccca gaagatgcgt tcacctaaca
    17821 gcatcgcatc aaagttaata gtcaagccac cgccgctacc ggcaaacttc gtcaactgac
    17881 gcagtgtgct acggtctgcc gggaagctct cttccagttc gtagcctttt tcagccgcaa
    17941 actccgtgaa gctcacttcg cttacgcctg ccagctcttt cgacagcgat tccagttcga
    18001 tctcttcgcc tgcctgcagc tgctcattac agtagctata aacctgctgg cgcacgttct
    18061 gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc
    18121 cacggttttg cgctttcgcg ttcaatcctt cgctggcccc gaggaaatcc ataaagaaat
    18181 ccgccacttt gcgccctact cgccctttca ggaaagtgag atagcgggtg gactctggat
    18241 tggtttccca ttcggttaaa tcgatacgcg caacaatatc cgcatgattg atatcaagat
    18301 aatgggttgg gttgatgtcg agattttcgt taacgcgcat actgctcagg ttgctcagaa
    18361 ctgccaccag caaatactcc accgccagat aacggtagtg gcaaaacagc acgaatccac
    18421 cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg accggttgcc gcgcggctaa
    18481 atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc agttcactct
    18541 cttcgctaaa caagccgtaa gctttatttt tggcgctata gacccgatgc agttctgcca
    18601 ccatctcaac gacggtttct gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt
    18661 tctgctcatc acgcttgata agctggtgca gagcaatctg gttgatatcc agactcatga
    18721 taaactctcc tttaagaccg ggcggtattc aaccaccgcc agttaagcga cgcaataaaa
    18781 gatggcggtc attttacaga aggcagcgta acgagaatga tttaacgcat aaaaaagcag
    18841 aaaaaaaacc gttgctacgg taatatgttg ccctttcatc aactaactga tttcgattta
    18901 tgccacaaat ttcccgctac agtaatgaac aggttgaaca attgctcgca gagctgctca
    18961 acgtactgga aaaacataag gctccgaccg acctttccct gatggtgtta ggcaacatgg
    19021 tcactaacct tatcaacacc agcattgccc cggcccaacg ccaggcaatt gccaactctt
    19081 ttgccagcgc cttacagtcc tctatcaacg aagacaaagc gcactaaggg aaacagataa
    19141 caggttatgg taactcatcg tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg
    19201 gggcactggt ttgcactgtt caatattctg ctttcgctcg tcattggcag ccgttacctg
    19261 tttatcgccg actggccgac aacgcttgct ggtcgcattt attcctacgt aagcattatc
    19321 ggccatttca gcttcctagt gttcgccacc tacttgctga tcctcttccc gctgaccttt
    19381 atcgtcggct cccagaggct gatgaggttt ttgtccgtca ttctggcgac ggcgggaatg
    19441 acgctattac tgatcgatag cgaagtcttt actcgtttcc atctccatct taatcccatc
    19501 gtctggcagt tggttatcaa cccagacgaa aatgagatgg cgcgcgactg gcagctgatg
    19561 ttcatcagcg tgccggttat tttattgctt gaactggtgt ttgcgacgtg gagctggcaa
    19621 aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc attcttattt
    19681 atcgccttta tcgcctcgca tgtggtgtat atctgggccg atgccaactt ctatcgcccg
    19741 atcaccatgc agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg acgtttcctt
    19801 gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc gtcttattga gcaaggtaat
    19861 ccagacgccg tttccgtaca gtatccgtta agcgaactgc gctatcgcga tatgggcacc
    19921 gggcagaatg tgctgttgat tactgtcgat ggcctgaact actcacgctt cgagaagcag
    19981 atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc
    20041 ggcaacacta cagacaacgg catctttggc ctgttctatg gcatctcgcc gagctatatg
    20101 gacggcattc tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc
    20161 tatcagctgg ggttattctc atcagatggc tttaccagcc cgctgtatcg ccaggcattg
    20221 ttgtcagatt tctcgatgcc gagcgtacgc acccaatctg acgatcagac cgccacgcag
    20281 tggatcaact ggctgggccg ctacgcacaa gaagataacc gctggttctc gtgggtttct
    20341 ttcaatggca ctaacattga cgacagcaat cagcaggcat ttgcacggaa atatagccgg
    20401 gcggcaggca atgtcgatga ccagatcaaa cgcgtgctca atgcactgcg tgattctggc
    20461 aaactggaca atacggtggt gattatcact gccggtcggg gtattccact gagcgaagag
    20521 gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc cattagtgat tcactggcca
    20581 ggcacgccgg cgcagcgtat taatgctctg actgatcata ccgatctgat gacgacgctg
    20641 atgcaacgcc tgctgcatgt cagcacacct gccagcgaat attcgcaagg tcaggatttg
    20701 ttcaaccctc aacgccgtca ttactgggtt actgctgcgg ataacgatac gctggcaatt
    20761 accaccccga aaaagacgct ggtgctgaac aataacggta aataccgcac ttacaactta
    20821 cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt tgttattgca agtgctgaca
    20881 gacgagaagc gttttatcgc taactgatta attataaatc agttagcgaa atatcttact
    20941 tgcaatcggt gtggaaaacg gtagtattag cagccacgag tcggcacgta gcgcagcctg
    21001 gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa
    21061 aatcccaaga aaaaaccaac ccttacggtt ggttttttta tatctgcaat taattcgata
    21121 aacagaccgt gacacatcac agcctgttta ttttctgtta tcagaacgtc cagaccacac
    21181 ccgcctgagt attccacggc gattctaccc ctgcgccatt gccatagctg actgacaaat
    21241 gaccgcttaa cgtcggggta aacgatgacc ttatcccagc ctgataaacg ccactggttc
    21301 cagccacatc attattaaat ttgccatcgt cattaacttt cacctggtta gaatcggcgt
    21361 attcctgacg cacggcggct ttcagccagg gttccagcgt cgtaccgttt tgcaggtcca
    21421 tgtgatagct taccgccgtt cccgcttcag cgcgtaatat ccgggtattt cccacatccg
    21481 cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcca
    21541 gatagggtct aacactccac aatccgtcaa cccaacggaa cccgctctca acatgagcac
    21601 ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc attactcatc ttgccatgga
    21661 tggtgttggc aaaacggtca actttcacca ccccatcaac ataggcaccg ttctgatgct
    21721 cccaaccggc ataagccccc agggtatagc tatcgatatt accttttccg ccgcgatcaa
    21781 aaccaatatc agaatgagag taaccaaaga acaagccgcg aattgtactg ctttcttcac
    21841 gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca aaaccagctc
    21901 ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga actccacatc accgtatcgt
    21961 aactaacgcc ttttacgcta ccaagacgct cacgcacggt gtccagttct gcatcaaata
    22021 ccagcggttg tgcggctgcc atgttcagca catcagtggt tgaaggggta atttgcgcgc
    22081 gattctctgc gaggctccag ctatggttgc cattatccag caaggtatat tcatacgtac
    22141 cgatatcaac aacgcctccg gcattgccca acgtaaatgc agcatcaccg ccgcccgttg
    22201 ttaccagtgt aaggctatct cctgctgccg ggctggcacc ggtgtccgtc acgaatattt
    22261 tgaaatcacc tgttgcctga ccggtgacgt tgagctgatc gctctgatga ttggccatat
    22321 cggtacgcat ataaaaattg ccgtttcccg acagggtatt ggtggtcaac gtaatgaaat
    22381 tttccgcact tgccgtcaca gatgaacgag tcactggctc agccatcata atcacatttg
    22441 cgccggacat cgcaaggttg gctaagttga ggcgagtgtt ttggttcgtc gtagtgatat
    22501 cggcaatgat ggtccatgcg ctattttcga gcgaaacgtc cgctttgctg gtatccacgg
    22561 caccatgcgt tctaaaagaa gcgccctcag aaaccactac cttacccacc aacatagcgg
    22621 gtgcataatt tatttgcggc ttcatgacct caagaatgcc atcattccct ctgactgaca
    22681 cgttaaccat tgtgccagcc cagacattag cgcggccatt gttgattacc atattttcag
    22741 agatggcttt tgaggaatac gtgatacttc cattctggtc tgatcgaccg agatcatata
    22801 ccgcatttgc ctgcactttc gtaccagtat cctttcccag cgattgcata gtggcatgct
    22861 catcaaggat agtgtcaatg gcctgcgtgt cctccataac aatgagcccg gaaccatcat
    22921 ccagttcata attttttgac acaccatcct ttatactaaa ttggccttta ctgtttgtac
    22981 cactcacttc cagcgcattc gttgagacaa ttaatttccc gcctgctttt ttatcaacgc
    23041 cagttgcagt cccgccatcc ataacctcca gtaagccgcc actatccaca gtagtattat
    23101 aagcgaagtc attttcttca actcgcaaac ttccgccgtt ttccaacaac atattattag
    23161 caataccatt cttgatatcg aactgaccga gacggtttgt tccgaatacc tccatggccc
    23221 gcgtggttgt tttaatagca ccgcctgttt tctgatctac cgcaagcgca aatcccccct
    23281 ctttaacctc cagtagtcct cccttttcga tagcggcaaa aacggccccc ccatcttttt
    23341 caacgattaa gcgcccgcct ttaatcgtgg tatcattcgc tgggccataa acctgtagca
    23401 caccattatt gagggtagtc tggtttgccg cgccacccat ttgaacaatt tgataaccat
    23461 catttaatac tgagtattct gcggtaccat tcatttcaat gtattgatac cctccgttaa
    23521 tttcagtatt actacttaca ccaaattctt ttatatgctg ttctccgcca ggattgattt
    23581 tcgtattatt ggcggtccca taaaccagct ggatatcttt ttccaggata acaccatcaa
    23641 ctgtctcacc atactcaact gttgatgcca atgctatggg tgaatataac gctgtagcca
    23701 ccagaacaga tattgcggaa cgacaaagag aaacagaacc agattgatgc attgagcttt
    23761 catcctatga aattaattgc agttaaaagc attgggtaca gaaaataccc atagctccat
    23821 acccggagtc agtttttaaa aactgtttaa agaaatgcac aagtattgtg attgattttt
    23881 tagttgtttt tcttgatgag aagctgatgc aaaattccgt ctttataata aaaatgatgc
    23941 caaagcgaac gacaaggttg tagttttcac tacatgtcca tacataaaat ggggtaacat
    24001 tcacgcgcct ggtagcgtta ccaacgctac gctcaaacat aatgattcta ataaaacctc
    24061 aggagactac tatgcctgaa gcaacgcctt ttcaggtgat gattgtggat gatcatccac
    24121 ttatgcgacg cggtgttcgt cagttactgg agcttgatcc tggctttgaa gtggtcgccg
    24181 aagcgggcga aggcgcgagc gcgatcgatc tggcgaatag actggatatc gacgtgatct
    24241 tgctggatct caatatgaaa ggtatgagtg gcctggatac cctcaatgcc ttgcgcaggg
    24301 atggcgttac cgcgcaaatt attatcctga ccgtatccga tgcctccagc gatgtctttg
    24361 cgctgataga cgcaggcgca gacggttatc tgttgaaaga cagcgacccg gaagtattgc
    24421 tggaagcgat tcgtaccgga gcgaaaggca gcaaagtctt tagcgaacgc gtcaatcagt
    24481 acttacgtga acgtgaaatg tttggcgcgg aagaagatcc cttcagcgtg ctgacggagc
    24541 gcgagctgga tgttctgcac gagctggcac aggggctgtc aaataaacag attgcatcgg
    24601 tgttgaatat ttccgagcag acagtaaaag tacatattcg caatctgctg cgtaaactca
    24661 atgtccgctc acgcgtggcg gccaccattc tgttcctgca acaacgcggg gcacaataaa
    24721 aatagcccga tggatttatc atcgggctga gatttatgac aaacgcaaaa ctgcctgatg
    24781 cgctacgctt atcaggccta cgtggatcga tcaatttatt gaatttacac aattttgtag
    24841 gccggataag gcgttcacgc cgcatccggc ataaactaag cgcactttgt catcagtcta
    24901 gcccgatggc atcaccatcg ggcctctttt tatttactct cctgcggcga caaatgttgc
    24961 atcgcctgcg cgatactacg ttcaatcacc gcacggcgag tatcgttggc aggtaagagt
    25021 ttcaacatca tctcccacgc ggcaacggct tcgccaaatc gctgctgctc aaaggcatta
    25081 aacgcataca tgcttagcac acggatattg ctatggtccg ttctcaccag ctgacgtagc
    25141 agttcaccgc cgaggcggtt gtcgttggga tcagatgaac gagtcaacgc ttcagcgtat
    25201 cccagtgcgg catcgctgtt cttcggatcg aggcgatacg cagtagcgta tgcatcggtg
    25261 gcgatactgg cgttacccag cgccatgcca acgcggccca acataatcca gccttctata
    25321 tctcccggat ttttttgcag ttgagtacgc atccccagcg caaggcgcga catctcttct
    25381 tcgttgagcg gatcggcttt cgggtccagc gccctgtcca gcaacgccgg agcctgtgcc
    25441 gtggcctgct gccagatttt cacctgctga taattgccag tctggtagta gctgacgcca
    25501 gccacaatta acgccaccac aatacccggc agataaacaa catatccggc acgcttacct
    25561 tccggcacgc tttgttcagg aaacgcttcc ggcaccacgc gtacccgacg ccgcgagcgg
    25621 gcgtatatga cccaaccgcc aatgccaata gccactactg gcagcaccca cagcagcacg
    25681 gtcagcggcg ttaacggcgg atcgtaagtg acgaagttgc cgtaacgcgc caccatataa
    25741 tcgacaatct cttttttact tttaccttcc tgcatcagtt catacacttt ctgacgcagg
    25801 tcggtggcaa tcatcgagtt ggaatcggca atgctgttgt tctggcattt cgggcagcgc
    25861 agttcttcag tgagctgacg gaactgttgt tcctgtgctt catctttaaa ctgcaacacg
    25921 tcgatggtcg ccagcgctga gccggagatc atcagcatca gcacgcccaa taaaaacctc
    25981 attgtgcggc ctccttactg tatttctccc acagcggctt gatctcttct tcccagacgc
    26041 gaggattgag atcgcccgca tggcgatagc gaatgatacc gttgccgtca ataaggaagg
    26101 tttctggcgc gccatagaca ccgagatcca gccctaacat gccgtcgcca tcaaacaggc
    26161 ttagcgcgta aggattgccc agctctttca gccagctgat tgccttctgg cgatcgtctt
    26221 tatagttcat gccgaccacg cgaatgccct gcgctgaaag ctgattcaga tattgatgtt
    26281 ccgcacggca ggtcggacac caagtcgccc agacgttaag cagtactggt ttgccctgag
    26341 tcagcacatc cgcctgataa aactgccccg gattgtccag tgattcgaga cgaaacttcg
    26401 gcacaggctt gccaatgagc gccgattcca gattggtcgg atcatcccct tcggcattac
    26461 gcgccagctg ccacagcagc gccgcggcaa tcgccaggaa gataatcaac ggaattaaca
    26521 atactttgcg cttcatacgg cctccggcgc agttttttgc ggactcacgc gcttacgata
    26581 gcgaggatca aacagacaca gcagtccgcc caacgccatc atcagcccgc ccgcccaaat
    26641 ccagcgaaca aatggtttgt agtaaagacg cacggcccac gcgccgtttt ccagctcttc
    26701 accgagcgcg gcgtacaggt cacgcgtgat gccgccgtca attgccgctt cggtcatcat
    26761 cgacccggca gtgttgtaat aacgtttttc cgcatacagc accgtttccg gtttaccctc
    26821 tcgcgttacg ccaatagtcg ccacaccgcc gcgccagttc gggccagtca cctctttgac
    26881 atcacggaag gtgaagcgat attcatgaat atcgacgcta tcgccggact tcatgcgcac
    26941 atcacgctca acgctataat tctggctaaa ggcaatgcca acaattgtca ctgccagccc
    27001 caggtgagcc gccaccatcc cccaataact gaaggtggtt ttcgtgccgc gtgaaatacg
    27061 cagcgcagct tccgcagttg ccagcaccgc aatccagcag gccattgcca ggccgagcac
    27121 cgtcatcgcc acaactttgc tttcgaacag ccacggcaac agcagagaca gcaccagcgt
    27181 agagatgaag gcgataatca ataaattgcg gattttacgt gggcgatcgc gcccccagcg
    27241 caccagagga ccgacaccaa gcagtagcgc aaacggcacc atcagccagg taaacatggt
    27301 gttgaagaac ggttcgccaa tcgaaatact gcccagtcca agttgcttgt gcaccagcgg
    27361 cagcagcgtc cccagcaaca ccaccagcat cgcggcgacc agcaaaacgt tgttcgctaa
    27421 cagcaaagat tcccgcgacc acagcgcatt gtttacgcgt gagcgaactt tgtgtccacg
    27481 cgcggcaaac agcagcagcg aaccgccaat caccagcacc ataaaggcga ggataaacat
    27541 accgcgcgcc ggatcagacg caaacgcgtg taccgatacc agcacgccgg aacgcacgag
    27601 gaaagtcccc aacagacaca acgagaaggc actgattgcc agcagtaatg tccacgcttt
    27661 gaagctggcg cgttgttcag tgaccgccag tgagtgcatc agtgcagtcc ccaccagcca
    27721 cggcataaac gaggcgtttt ctaccggatc ccagaaccac cagccacccc agccgagttc
    27781 gtaataggcc catgcggaac cgagcacaat gccgagcgtt aggaaaatcc acgccgccag
    27841 cgtccacggg cgagtaaaac gcgcataagt gctgtccaga cgcccgctca gcaaagaagc
    27901 aatggcaaaa gcaaacgcca ccgagaaacc cacgtacccc atataaagca gaggcggatg
    27961 gaagatcagc cccggatcct gtaacagcgg gttaagatcg cgcccttcaa tcgggaagtt
    28021 cggcagcgtg cgtgagaacg ggttagaggt aaagagaatg aacagcaaaa agccgacgct
    28081 gaccatcccc attatcgcca gtacacgggc cacaatatcc agcggaatac gctgactaaa
    28141 aatcgccacc gcaaaggtcc agccgctcat cagcagcacc cacagcagaa gcgaaccttc
    28201 atgcgcgccc caagtagccg ccacgcgata ccacaccgga agctgggtat tggagttgct
    28261 ggcaacatag gtgacggtaa agtcgttgac cacgaaagca ttgaccagca ccagaaatgc
    28321 gccagccaca gacataaaca gcagccaggc aaacaagcgg gaagacgcca tcatgcgcgc
    28381 atctccgcgc gccacgcccc atagcggata cacggacagc agcagcgcaa tccctaacgc
    28441 caagcacagc agcccgtttc caatttccgg catcatgatg ctgggtcctt ataaacactc
    28501 gccgggcgac ggtggttagc ttccatcgct ttctcaactt ctggcggcgt gtagttttcg
    28561 tcgtgtttcg ccagcacttc tttcgcgagg atatgattgc ctttttccag ctcgccctgc
    28621 accacaacgc cctgcccttc acggaacaga tccggcaaaa tgccttcgta agagacatcc
    28681 actgagcctt cagcatcgta aatggtgaag gtcactttca gcgaattggg atcgcgctgc
    28741 acgctacccg gcatcaccat cccgccaacg cgcagacgct gaccgacttc cggcatctgc
    28801 tgagtttcac gcttgccgta gagaatttcc cccggcgtat aaaagagatc gatattcgag
    28861 cgcagcgcat atagcaccag accgatagtc agcgctagcc ctgccaacac ggcacaggca
    28921 atccacaagc ggtttttacg gcgaatattc atgcagcctc ctgctgttgc gcagcacgta
    28981 aacgcgcctc acgcgcccgc tgttgcgcca caccacgcag aattgcgcga tgttgcatca
    29041 ccgagtgcac gaccaaaacc accagcggaa taacggtcat caccaccgcc agccagacaa
    29101 aaaaggcgta accgcccatt gcgaaaaatt cattccagga agcaaatgca ggggtcattt
    29161 acggcctctt ttcagtatca gttcactcac ccacggacgg cgtttttcca tcagcaaaat
    29221 caaattacgc atccgcatca gcgtcagcgt ggcagacagg agcaggaagc caaaaatcga
    29281 ccagcgcagc ggcgaacgca tcgccggatc gatactttgc tgcatccgcg ttgatccctg
    29341 atgcagggtg ttccaccact ccacggagta atgaataatc ggcagattca ccacgccaat
    29401 cagcaccatg atacctgccg cacggcccgc cagacggcgg tcgtcgaagg cgtgccacag
    29461 ggcaatcaca cccacataca aaaacagcag caccagttca gaagtcagac gtgcatccca
    29521 tacccaccag gtgccccaca tcggttttcc ccatgcagag ccggtaacca gggcaataaa
    29581 ggtaaacacg gcaccaatgg gggccatcgc cgccaccgcc aggttggcca ttttcatctg
    29641 ccagacaagg ccaataaacg ccgccaccgc cattgatgca taaatgccca tcgaccagat
    29701 cgccgcaggc acatgcaggt agataatgcg gtagctattt ccctgctgat aatcagccgg
    29761 agcaaagccg aatccccaga tccagccgac ggtaagcacg accacactgg caattgccag
    29821 ccacggtata aaccagccac agatttgata cagccgtggt gggatcgcca gttgatgcag
    29881 tgttttccac atagtttcga taccagactc gaacaaaaat cagtaatcca gcgttattga
    29941 atgctgattc gtaacgctgc cgccgtcgca aaaggactta atgtcgcggt gcctgccagc
    30001 aacgcgccta aaattgccag atacccatca acgggcaaat gcatagaagc cgcgtccatc
    30061 gcggcggtgg caaagatgag taatgggata gtcagcggta acaccagtat gctgagcagc
    30121 acaccaccgc gcttaagtcc cactgtcagc gccacgcccg gtgcaccgag aaagccaagc
    30181 gtaggcgttc ccagcagcag cgtcagcgcc atcacttgcc agccataaac atccattccc
    30241 agtagcattg ctaccagtgg cgaaaggatg agtaacggca gaccggttac catccagtgc
    30301 gccatcacct tcgccagcac aacggcgggc aagggtaacg gcaacaacat caattgttca
    30361 agactgccgt cctgcaaatc gtcacggaac agtcgttcca gcgccagcaa ggatgaaagc
    30421 agcgcagcaa cccagataat gcccggtgca atacgcgcca gcagttgcgg ctccggaccg
    30481 atactgagcg gaaaaagggt aattacaatc aggaagaacc acagcgggtt ggcgatttcg
    30541 gcgctatggc gaaacgctac gcgcagttca agacggaaaa tacgccagaa catcatgcgg
    30601 ccctcgtttg cgtcagtgaa atgcggcgaa ttttactttc agcaacgttg agcggctggt
    30661 gggtagtcag aatcacaatc cccccctgct ccgtatgctg cgccatacgc tgggtcagac
    30721 gatcgacgcc gttaacgtca atcgcggtaa aaggctcgtc gaggatccat aatgtggcac
    30781 gggtcaacca cagacgcgct aaagcgacgc ggcgttgttg cccggcagaa agttgattta
    30841 ccggaatatc ttcgaatccg gcaagcccgg cctgcgccaa ggcttccaga cattgtgcgg
    30901 catcgccatc gcgatgataa aagtgcagat tttctaacgc cgtcagccgg gttttgatcc
    30961 ccggctgatg gcctatccat aacagggttt gatggtagct gtcgcgtacc tgatgcaagg
    31021 gctgcccttg ccagagaacc tcgccagcgt cagggcgaga caaccccgtc agcaaacgga
    31081 gaagcgttgt cttccccgcg ccgttgctac cggtgatttg tacccactct cctgcgttca
    31141 gcgtaaatga caagccacta aataaggttc gttcatcccg ctcacaaagt aactctctgg
    31201 cttcaagcat acccacgcaa taaccctgtt aaaaacctgg ctcgacttca cgcatatcgg
    31261 gcagcttgtg cgcgatccct ttatggcaat caatacaggt ttgcccatct ttcaccgcct
    31321 ggtcatgcat cttcgcggca accgatttct gggcggttgt atccatatac tcgaagttgt
    31381 gacagttacg gcactcctgc gagttattgt ccttcatgcg ccgccactca ttctgtgcca
    31441 tcgtcagacg atgagcttca aatttctgcg gcgtgtcaat aacgccaaaa attttaccat
    31501 acagctcttt acttgctttg agcttgcgta tcatcttcgg cacaaactcg tgcggaacgt
    31561 gacaatccgg acaggtcgca cggacgccgc tacggttgtt gtagtgcacg gaatccatgt
    31621 attcctgata caccgtgttg cgcatttcgt ggcagctaat gcagaactct tcggtattgg
    31681 ctttttccat cccggtgtta aagccccccc agaagacgat gccgccaaca aaaccgatca
    31741 acagcagcgt ccccagcgcc agatggctgg gggtacgcca ccatttccac aggcgcttaa
    31801 tcagaccagg cttacggtca gaatttccca taataacctc ttatttcccg taaccttttg
    31861 atggggtaaa ggtattcccc acgattggcg cggtatcggc ctgcggtacg tgacattgca
    31921 gacagaaata acgacgcgga gccacttccg cgcccacttt gccgtcgctg tccataaagt
    31981 gagtaggact gatacgcggc gcgccagtgg tgcgatagct ttcgacaccg tggcactgca
    32041 agcagcgatt ggtattggtc gttacctgat aaccttcaac gctatgcggg atcattggcg
    32101 gctgattcac atagttcagc ggcatccgat cctgctcttt tggcatccga atggcccctt
    32161 cctgcgtccc ggatacttcc ggcgactggc taaaatcgac gccattagcc gcccaaaccg
    32221 cgccgcttac caccagggcc agcatcgccg tccattgaca cagcgctttc ttcaggtcat
    32281 ggcttttcat gatttcgctc ccgaactcca tcgtgtagtt attgtaaata catcctcaga
    32341 acagacatcc acgcagcgac cgcacgtcat gcaatcgcgg ctggtgacct gcaccgggct
    32401 ttgttcatcc agcaccgggg cacgtagcac atgcggttcc gggcaaacat gaaaacaatc
    32461 catacagcgg ttacatttct gccgatcgct ggccgcaacg gtaatcacac ctttgctacc
    32521 cagcacgcca tacagcgcgc caaccggaca aatgtgcccg caccagccgt gttcaacgac
    32581 cagtagatca aataaaaaca gcgcgagaat aagcagcgcg ccgctgccga agcccattac
    32641 caggctacgc cccatcagag aaacggggtt gatccattcc caaatgagcg tgccagttaa
    32701 ggctgatccc accagaatga ccaccaacag cacgtagcga atgtggcgag ggatcgtcgc
    32761 agactgattg aggtcaaagc gcctgcgtaa ccagttcgct aagtcggtaa tcgggttcag
    32821 cgggcagacc cagctgcaaa ataatcgctt tcccgccagg gcgtagagca cggtgataat
    32881 caccgcaccg gtcaacgcca ccgtggcagg cagatgacca ctggcaagac tttgcagcgt
    32941 catcagcgga tcggtcagcg gaacggtgtc gaataacagg ctactgctgt agttgccgtg
    33001 caagatccac acaccaaacc atggaccgct caaaaacatc cccagcacga agaactgaca
    33061 aaggcgacgt aacaccagcc aacggtgact gcgccaccag ccttttttct ccagcgcctc
    33121 gcgcccggcg tcacgtttac gatttgccat tgttcccctc cagccagccg aagcggtaat
    33181 ggtgacctaa ctcccctttc gccagtgaca gcggtaacac cttgattgcc ggttgttcca
    33241 gcacgcatac tttttcgcac ttaccgcaac cggtacaggc gtcgctgtga accgtcggca
    33301 gaaagcgggc gtgcttaccg gtacgcgtgt tgcgctccag ctccaaggtg atggcctcat
    33361 caattttcgg gcattcgcga taacaaacat cgcagcgcag cccctgaaag ttgagacagt
    33421 tttcctggtc caccagtacc gccagcccca tccgcgcgtc gtcgatcgat tcaatctcac
    33481 gatccagcgc accgcttggg cacactttgg cgcacggaat gtcctcacac atttcgcaag
    33541 gaatatcccg tgcgacaaaa tatggcgtgc ccgccgacag accagaggcc agcgtcgcca
    33601 gttttaaggt gtcgtaaggg caagcctgaa cacactgacc gcaacgcaca caggcactgg
    33661 caaaggcgtt ctcgtttatg gctccgggcg ggcgcaaccg cacgccagat gcgcgtgcgg
    33721 tttgctgttg taaccccagc gccacaccca cggcagccag cccgcctgct gtgcgaacga
    33781 catcgcgcag aaagcggcgg cgaccatttt gaggtttcgc tgaccgggac ataatggctt
    33841 acaccttctc cagtttgacc gcgcacttct tgaagtccgt ctctttcgag agcggatcgg
    33901 tcgcatccag cgtcagtttg ttaaccagct gtgcggcgtc gaagaacggc atgtacacca
    33961 ggccctgtgg cggacggtta cgaccgcgcg tttcaacaat cgagatcact tcgccacggc
    34021 gagaaaccac tttcactttg tcgccacggc gcagatcgcg cgctttcgca tccagcgggt
    34081 gaataaacaa gaccgcttcc gggaaggcgc ggtgcagttc cggcacacgg cgagtcatac
    34141 tgccggtgtg ccagtgctcc agaacgcgtc cggtagagag ccacaggtcg tactcttcat
    34201 ccggtgcttc cgccgccggt tcgaacggca gtgcgaagat caccgctttg ccatccggtt
    34261 taccgtagaa cttatagcct tcgcccgctt tcacgtacgg gtcgttacct tcgctgtaac
    34321 gccactgtgt ttctttacca ttcaccaccg gccagcgcag accgcgcgct ttgtggtagt
    34381 catcgaacgg tgccagatcg tgaccgtgac cgcgaccaaa ccatgcgtac tcttcgaaca
    34441 gccctttttg cagatagaag cccagctcgc gggattcatc gttcagctga tcttccgcca
    34501 gttcggatac cgggaatttg ctcacttcgg gggtggcata cagaacttcg tacagcgttt
    34561 tgccacgcag ttccggtttc ttcgccagca gctcttccgg ccatacttct tcagttttga
    34621 agcggcggga gaactggact aactgccaga gatccgattt cgcttcgcct ggcgcctgta
    34681 cctgctgacg ccagaactga gtacggcgtt cggcgttacc gtaagcgccc tctttctcta
    34741 cccacattgc ggtcggcagg atcaagtcgg cggccagcgc actgactgtc ggatacggat
    34801 cggagacgat gatgaagttg cgcggatcgc gccagcccgg catacgctct tcattgatgt
    34861 tcggcccggc ctgcatgttg ttggtacaca tggtccagta aacattcagc ttgccgtctt
    34921 tcagcgcacg gtcttgtgcc accgcatgaa gaccgatttt cgccggaatg gtgccgctcg
    34981 ggatattcca cttcttctca cagatatcac gatgtttctc gttagttacc accatgtccg
    35041 caggcagacg gtgagcaaag gtgcccactt cacgcgcagt accacacgca gaaggttgcc
    35101 cggtcaggga gaacggaccg caacccggct gggaaatttt gccggtcagc aggtgcaggt
    35161 tgtagaccag gttgttagcc cacacaccac gggtatgctg gttgaagccc atcgtccagt
    35221 aggagatgac tttcttgttc ggatcggcat acagctgcgc cagttgttct aactggtctt
    35281 tcggcacgcc ggtcatttca gcggtttttt ccagcgtata ctcagcaacg aaggctttgt
    35341 aatcttcaaa gctcatcggt tcggaggcgt cagaacccgg attcttcgct gctttttcca
    35401 gcggatgggt cggacgtaaa ccgtagccga tgtccgtcgc ccctttgcgc aggttaacgt
    35461 gcttgctgaa gaagtcctga tttatcgcat tgttttgaat gatatagttg gcgatgtagt
    35521 tcaggatcac caggtcagat tgcggcgtaa agatgatgcc gttatccgcc agctcgaagc
    35581 tacgatgctg gtaggtagaa agcaccgcca cggtgacgtt ctggttagag agacgactgt
    35641 tagtgatgcg tgaccagagg atcgggtgca tctccgccat gttagcgccc cacagcacaa
    35701 acgcgtcagc ctgctcgatg tcgtcatagc agcccatcgg ctcatccata ccaaaggtac
    35761 gcataaagcc aactactgcc gacgccatac agtgacgtgc gttcgggtcg atgttgttcg
    35821 aacggaagcc cgctttgaac agcttggacg cggcataacc ttcccagata gtccactgac
    35881 cagaaccgaa cataccgatc gattccggcc ctttttcttt cagggcggtt ttgaatttct
    35941 cttccatcac atcgaaggcc tgatcccagg tgattggggt aaattcgcct tctttgtcat
    36001 atttaccgtt tttcatacgc agcagcggct gcgtcaaacg gtctttaccg tacatgatct
    36061 tgggcaggaa atagccctta atgcagttca ggccacggtt aaccggtgcg tccgggtcgc
    36121 cctgacaggc caccacacgc ccctgctgcg ttccgaccag aacgccgcaa ccagtaccgc
    36181 agaaacggca cggcgcttta tcccatttaa tggcttcctg ctgaccaaca acggcgcggg
    36241 caacgcccgg cacgctgaga ccggcagccg ccgcagcggc cgcaacggcg ttagctttca
    36301 taaagctacg acgactgagt ttcatggtgt ttcctcacct tgctcttcct gctggtgata
    36361 aaccagcgac accgccagca cgccctctac gttgcgtact gactcaatgg tttggatcag
    36421 cgtttcgctg tcttctgctt ccaccaccac aatcaactga ccgctcggcg cgtcgctgac
    36481 agcaacttca cagccgggaa aggcgttcag ttgggtgctg atgtctgaaa ttcgttcgct
    36541 tttggcctgc acgaccaggc tgcaaacttg ccagttagtg tgcatggaga tactccgcag
    36601 ttatggctga taccggacag ctggctgcac atgctccaca tccgttgcaa agttggctat
    36661 taagttgcgc ctggtagatc ccggagagcg tcgggcgaaa gataattgcc attggctcac
    36721 agctatcctg acagcggcgg cattcaactg actgatacgc caggcacgcg tccccgatgg
    36781 tgaactgcaa atcccaggcc ctggtgtggc gcggagaaaa tagcgattcg gggcaagcct
    36841 gcgcgcaggc gtaacagaag ctgcactcgt tatttttgaa attaacgctc ggatagccac
    36901 ccgtgccgcg ttgcagaatg ttgttttcac aagcattaat acaggcgtcg cagcgggtgc
    36961 aatgggtcag aaaatgagat tcatcaccgg accagggcgg acggataccg ttactggctt
    37021 tgcgccagca accagtgagt atgccccgac gggatgcatc aatcttcaca ttgaccttcc
    37081 atcattaacg cgctctgaaa attgagagcg accaaataga ccgcataatt aataagccat
    37141 ttttatagcc gctaagatat taaaggatgt gtcaaagatg cataccccga tcggggtaaa
    37201 acctgtagca ggatcaaaag agtggcggga agcgtggcaa aaacgggctt ttgctcacat
    37261 ttcaaatggt tataaacata tttatatagc gattaattca ccagagatat ttctgctggt
    37321 ttgctccctc atcagaattt aacactaaaa gagcaggtaa aattgtctga atgttcttta
    37381 agttattcat aaagcaaatt aataaatctg atgaatatgt taaccttcag cgacatcatc
    37441 ggtgaaaacc tataaatgaa gaaggaaagc aaaaaaatga agaccattct acctgaagta
    37501 ttgtttgccg ctttcgctac cacttccgcc tgggcggcag aaagcgtcca gccactggaa
    37561 aaaatcgcgc cttatccaca agctgaaaca ggcatgaagc gtcaggtgat tcagttaacc
    37621 ccgcaagaag atgaatctac cctgaaagta gaactgttaa tcggtcagac gctggaagtc
    37681 gattgcaatt tgcatcgtct cggcgggaag ctggaaagca aaacgctgga aggctggggc
    37741 tatgattatt atgtctttga taaagttagc tcgccggttt ccacgatgat ggcctgcccg
    37801 gatggcaaga aagagaagaa atttgtcacc gcgtatctgg gcgatgctgg aatgctgcgt
    37861 tacaacagca agctgccgat cgtggtgtat acgccagaca atgtagatgt gaaataccgc
    37921 gtctggaagg cggaagagaa aattgacaac gcggaagttc gctaaactgt tgtgaagtac
    37981 ggcaccccgt tggttggata aggcggttac gccgcatcca acatctaacg cccgagccgg
    38041 ttgcctgatg cgacgctggc gcgtcttatc agtatacgcc acatcattac aacgcaatat
    38101 ccgccacttc tttttgaacg ggttgcggtt tcaactgcgg tttcggcgta ctatctgctg
    38161 cttgcggttt gtcgtatttc agccccagca cttcgctggt gtactgcaac tcgcgttctg
    38221 ttgccgctac atcaccgttc agcttgcgtc cataagacgg aacgatcgct ttcaacgtag
    38281 cctgccattg cgggctggaa acgcgatcgc caaatacttt ttccagtaga ttcaacataa
    38341 tcggcgcggc ggttgacgcc cctggcgatg cccccagcag cgccgcaatg gtcccttgct
    38401 ggtcactgac cacttcggta cccagacgca gtacgccacc tttctcggca tcacgcttga
    38461 taatctgcac acgctgcccc gcttgccaca aacgccagtc ctcttttttc gcttgcggat
    38521 agtactcttt caacgcttca aaacgatcct cttcactcaa catcacctga ctcaccagat
    38581 atttcaccag atcgaaatta tccagcccga cgtgcatcat cggcatcacg ttagaggtgg
    38641 tggtggaact catcagatcc cacaatgaac cgtttttgag gaatttggtt gagaaggtgg
    38701 caaatggccc aaacagcact acgcgtttac cgtccagaac acgggtatcg atatgcggaa
    38761 ccgacatcgg tggtgcgcca acggatgctt taccgtaaac tttcgccaga tggcgattaa
    38821 ccacgtctgg attttccgaa acaaggaact gtccgcccac cgggaaaccg gcgtagtctt
    38881 tcgcttccgg aatccccgat tcctgtaaca gcttcagcgc cgcaccgccc gcgccgataa
    38941 agacaaattt ggcgcggatg ttctgtgcag tgccgttttt cagatcggca acggtaacgg
    39001 tccaggtgtt atcgtcatta cgctttaggg cgcggacttc gctgctgagt tgcagcgaga
    39061 agttagattt cttctgtaag gaagcaatta actggcgggt gatctcacca tagttcacat
    39121 cagtgccaat ttctgttgcg gatcgcgccc ttccatcact aacggtgccc actctttgat
    39181 ctgcgcgtga tcttcagagt aacgcatacc gcgaaacagc gagctttgtt gcaacgcggc
    39241 gtaacgggcg cgcaggaaat taacgttatc ctcgccccag acaaagctca tatgcggaac
    39301 ggtattgata aatgaacgcg gagtacgcag cacgccgcgc tcgacctggt gcgcccagaa
    39361 ctggcgggaa atctgaaatg cttcgttaat ggcgactgct ttttcaatac tgatgctgcc
    39421 atcggcgttt tgcggggtgt agttcagttc catcagtgca gaatgcccgg ttccggcgtt
    39481 gttctagccg ttcgaactct cctgcgcgcc cccctccagg cgctccacca tggtcatcga
    39541 ccattcaggc tccagctcgc gtcaataggt ccccaacgtg gcgctcataa tgccgccgcc
    39601 aatcaacagt acatcagttt cctgctcctc ggacgctttc gcttttgccg ccatcgaaac
    39661 ggcattaagc cccacggcca tcgagaagag catggcagtc acttttttca tcttgttaat
    39721 gccttacttt tagtcgcttt attgcaggtg agatttgcgc ggcatcaacg gtaacactta
    39781 agtaacaaca tttaaataat gtttataaat tatattctaa ttttagaaac attataattt
    39841 cgttgattaa ttatagggtt attagaaatg aagggatttt tagcatattg acctgacggc
    39901 agcagtccgc caggtcagcg tcgtaatatt atgccgtccg ggcaacggca tcacgcgaag
    39961 cggcatcgcg ctcttcgccc gtcagctcgc taagttgccc attgcgcatt tccagcaggc
    40021 ggtcggcgtg gataaagtaa tgatcatcat gactgatagc gaaaatagtt ttacccatct
    40081 cctgcatcag cggcagcaac acctgataaa actcacgacg gaagtgtgga tcctgatcca
    40141 ccgcccattc atccagcagg ataatatcgc gttcttctgc cagcgccagc aacagcgcca
    40201 cgcgtttttt ctgccctttt gataacttca ggttaacaat acgcccgttg cttaactcaa
    40261 gcttatgagc cattttcagc tgcgccagcc acttctcaac cagttgcggg ttagcgggtt
    40321 gcccctccgg ccccagcagt tgatcaaaca gccagacatc ggtaaacact gccgaaaaca
    40381 gtttgcgata atcttccggt tgttcgccgc tgacaggttt gccatccagc aagatttcgc
    40441 cgctttgtgg ctgatacaag cccgtcaaca acatcgccag cgtcgatttt ccgctaccgt
    40501 taccgccaat cagaaacagc agctcgccac gtttgatggt gagattaatc ggaccaacgg
    40561 aaaacgcgtt atcctgataa gcaaacgtca cgttacgcag ctccagcgtt tgccagttgg
    40621 gaaacgcctg cgggcgcgga aactcagctt tgaaaggcgc gagcgcgaat ttgttcagct
    40681 tgttaaacgc cacctgcgcc gtcagcagcg tcggcaatgc gccaaccgcc gaaagcagcg
    40741 gcgtacgcag gaataacagc gtcaacgaat aggtcgcagc aacgttggta tcagcccaac
    40801 cgagactgtt cgccatccag aacaccaggc cgatagcgcc cagcatcatg atgtttgacc
    40861 agttcacggc actaagatgg aaggtgtctg cgcgaataat atgatggcga tactcttgcg
    40921 catcggggat gtagaggttg ttaaacacat actcggcgcg ttcccggttc agagtcagct
    40981 ctttgcgccc ttcaagtaca gtttgaaaat ccgtgtacag cttgtcttcg gtttcacgta
    41041 gggtcgccat atgtttgtac acccgcgcca ccagcacaaa accgccccag atggtgatcg
    41101 ccatccagat agccgttacc agcaacattt tgcccgacag catccacaga tacgccgatg
    41161 aaccgatagt gagaatgatc ccctgcccca gttccggcag acgcacaaaa gcaatggtga
    41221 tattgcgcac atcgctggtt aatcccgcca gcaacgaggc gctaccgagt tgttcaatgc
    41281 gctcgacgtg ggtatccaga atccgcttga taaattcact acgcagtcgg taaacgaagt
    41341 gatgccccaa agtggtgagc gccagttgcg atccgagagt gactgccatc aacagcagca
    41401 ataatcccag aaactccggt aacaccagca gactggtatc cgccgtttcg ataaggcgct
    41461 gattgataaa agcaattaag ccaatgccta atgccgcact ggcgaggctt agcgccatca
    41521 cactgataaa tggccagcga tactgccgcc agacaagtac aagaagttcc atgcagaaaa
    41581 cccggacaat gaattacagc ccgcagttta aacatcttcg cgcgcacagc aataataatt
    41641 cttattttta ttctttttta cctgcctgac ggaatgtcag gttgtagcgg cagtcggtgg
    41701 tgagtggatg aaaccccgct ttcaacggtt gaataccgtg ataaaacagc cgcgattcac
    41761 cgccccatac caccacatcg ccatgttcca acaacaaacg tttgagcgga tcattacgtt
    41821 tcaggccacc aaattgaaaa atcgcgggta agcccagaga aacagaaaca attggcgcgc
    41881 gcagatccgg ttcgtcttta tcctgatgca gcgacagttt cgcgccagga gcgtagcggt
    41941 tgataagaca agcatctggc tggaaatctg gatagcccgc cgccgtagcc gcacgttgac
    42001 ataaatcatg aaagctttgt ggcatagcgg gccacggttt atttgtttgc ggatcaatgg
    42061 gcgaatagag ataaccttgc cgatgggtcg tccagcccag atgcccacag ttggtcatcg
    42121 ccaccgacat ggtatatccg cctggagcga ccatctggcg aaacggcgac tggctggcaa
    42181 cgtcattaat atcgcggatt agttgctccg cagcattaaa agcaaaacgc cgtaaaatta
    42241 ccgcaccagc cgccagtggc tcttgccacg gttcagcatc ggcaaacaga tccaacatta
    42301 cctctcctca ttttcagctt cgcggcgcag cagttgcgct ttacgcgaca cgccccagcg
    42361 gtaaccggaa agtgtgccat caccacggac cacccgatga cagggtatta cgatagccag
    42421 cttgttggcg gcacaggcgc tggcaacggc ccgtaccgct ttcggtttgc cgatggcgtt
    42481 agccagttgc tgataactga cggtttcacc acaaggtatc gtgcgcagtg cctgccagac
    42541 ttgttgctga aaagcagtgc cgcgaatatc cagcggtaac gtcagcggcg tatcgcgttg
    42601 attgaggctg gcgatcactt cacgcacatg ttgctgaaac gtcagatcgg caggcgcgtt
    42661 gtcggcagcg ggaaacatct gctgcaactc gctgattaat gtcgcgtcat catcgcccag
    42721 caatatcgcg caaatccccc gctcgctttc tgccaccagg caacgaccca gctcacaatc
    42781 agccagcgcg taacgcaccg ccagattttc gccaccgtga cggaattgtt tagccgtcat
    42841 gcccagcgtt tcatcggctt tgcggtagta actgctgctg tcggggaatc cggcgttaag
    42901 aatagacgtc gtcacgctct cccctttcgc cagcgattcg cgcaaacggc gagcgcgcca
    42961 ggcctgttgc caggctttag gcgtcattcc ggtagtagct ttaaacaacc gatgtagatg
    43021 aaatggactc atcgccacct ggtcggctaa ggcttccagc gttacaggcg tttcctgttc
    43081 cagcagtcga cacgcgtggg tgattttatc caaccgatgt tgccggggat tggctttgtc
    43141 tggctgacaa cgtttgcagg ggcgaaatcc ggcggcgagt gcctcgctgg catttgcgta
    43201 gaaggagaca ttttcccgca aggcatgtct ggcgcggcaa gaaggacggc aaaagatacc
    43261 cgtggtgcgc acggcgaaaa cgaattcgcc gtcggcattc gggtcgcggg ctaagacaga
    43321 ttgccagcgt tgatcgtcag ttaagcatgt ggcgtttttc ataatcagct ccctggttaa
    43381 ggatagcctt tagtctgccc ggtcaccatc acgcaaaaac caacaatctt gcgctttaat
    43441 ttttttcgct gacaaggaag cttttaaact gtggtgacat ccaggtttta aagctatcgc
    43501 cttctttggt gatcatataa accgccagcc cttcccggcg aacaacctct ttggctttct
    43561 ccggcccgag taccatcaag ccagtatccc aggcatcggc ttccagcgcc gtcggagcaa
    43621 tcaccgtcac ggataccaga ttgtgttcga tgggacgccc ggtttgcgga tcgataacat
    43681 gggaaagacg tttgccgtcc agttcgtaat agttacggta gctgccagag gtgctaatcc
    43741 catgaccgtt gatatccacc acagcctgaa ccgcgttttc tttatcggtt ggtttttgaa
    43801 tcgctacccg ccacggctgg ccttcaccgt tcataccacg gctgttcagc gcgccgccca
    43861 ccgacaccag atagcgggaa atcccttcct gctccatcaa gcgtgccagg tgatccgccg
    43921 cataaccttc accgacggtg gagagatcga catataaatc cggcaggtct ttttgcagat
    43981 attgctgatg cgactgattg atgaccgtca ggtgctgtaa gccggttttg gctttcatcg
    44041 catcgatctg ttcctggctc ggaatttgaa ccggctgttg ttccgggcca aagccccaca
    44101 gattcaccag cggcccgacg gtaatatcca tcgcaccatc ggtccttgcc ccaatgcgca
    44161 gcgaggtggt gacgatatcg gccatcgctt cacttaccgg ccacggcgac aaactttgcg
    44221 agtcgttaaa gcgcatcagc gcggaatctt ttttataggt cgaaagcagc tgatcgtcgg
    44281 cgtccagctg ggtctgaatc ttttctttaa gttcggcact gcgtttggca tcaatgcccg
    44341 ggatgctggc acgccagaag gtacccatgg ttttgccttc aagaacggta acttcagtag
    44401 cgtgggtttt ggcgggttgt ggtttttgat cgcaaccaac aaagaagagc gcggcaacca
    44461 gcagtgccac gcgggtaaag cttatttcca tacgtgatta tcctcatgcg aacggtcgca
    44521 agagtacacc aaaaaactgt gtttgtacgc tgaaaacaat gaaaaaaggg cccgcaggcc
    44581 ctttgttcga tatccatcga gattagaact ggtaaaccag acccagagct acgatgttat
    44641 cagtgttgat gccagcgtca cgagtgaact ggttgtcgtc cagcaggttg attttgtagt
    44701 caacgtaggt ggacatgttt ttgttgaagt agtaggtcgc accaacatca acatatttca
    44761 ggatatcttc gtcgtcgtag ttacgaccat tgatgacacc caggttttta cctttagact
    44821 gcaggtaagc cagggacgga cgcagaccga agtcgaactg gtactgagca acagcttcga
    44881 agttctgtgc tttgttcgcc caacccaagt tgcctacgcg agttgcgttg taggtctggg
    44941 tgtactgagc agctaggtag atgttgttcg cgtcgtattt cagaccaccg gtgtaggttt
    45001 cagcgcggtc gccattaccg atgtaacgct catcataacg gttcagaccg aagttctggt
    45061 catcagtacg tttggagctg gaaactgcag caccgatacc gaagccttcg taatcataag
    45121 taatagaacc gccaacgccg tcaccgttct gacgcagagc ttcacgaccg ttgttggtca
    45181 tgccttcacc ttccgggcta ccgtttttgc cctgatactg aacagcgaag ttcagaccgt
    45241 caaccagacc aaagaagtcg gtgctacggt aggttgcgaa gccgttacca cgctgctgca
    45301 tgaagttgtc agaaccgtaa gtgtcaccac cgaattctgg cagtacgtcg gtccaggaag
    45361 ttacgtcgta aactacgccg tagttacgac cgtagtcgaa agaacctaca tcctggaatt
    45421 tcagacctgc gaatgccaca cgggtccagg agttgttttc gttttcagcg ctgttgccct
    45481 ggatctgata ttcccactgg ccgtaaccgg tcagctggtc agtaacctga gtttcacctt
    45541 tgaagccaag acgcatgtag gtctggtcgc catctacaga cttgtcgtca gagaaatagt
    45601 gcaggccgtc tactttaccg tacagatcta atttgttgcc gtctttgttg taaacttcag
    45661 cagcgtttgc tgcgcctgct accagcagag ctgggaccag gagggacagt actttaactt
    45721 tcatgttatt aaccctctgt tatatgcctt tatttgcttt tttatgccac tgcatactga
    45781 ttaaccctca ttaatcagtc ggcaagtcca ttctccccaa aaatgcagaa taatccaaca
    45841 cgaatatgat actaaaactt ttaagatgtt tcatttatcg ctatagatgt ttcaaaatgt
    45901 aaatacaagg gaacttttta agattattgc ggaatggcga aataagcacc taacatcaag
    45961 caataataat tcaaggttaa aatcaataac ttattcttaa gtatttgaca gcactgaatg
    46021 tcaaaacaaa accttcactc gcaactagaa taactcccgc tatcatcatt aactttattt
    46081 attaccgtca ttcatttctg aatgtctgtt tacccctatt tcaaccggat gcctcgcatt
    46141 cggttttttt acccttcttt acacactttt cattattctg tgctaccaca gaaaaactat
    46201 aacgcttgtt aactatttca caaataatta acatcagcat aatttccagc aatctttgtt
    46261 tatttgcaat taatttcgtt gggctttttg taggttattt gtacagcaaa atggcgcttg
    46321 tacatctatt tccccccata caggatgata aatatcacgg gagaatagag aatcatcaat
    46381 caggtaagag tctggaattt cacactgtac cctttatact gccctatcac ttcgcgaagt
    46441 tttaacaggt cataaacacg aatgcgtcag aaagagacaa cggccacgac ccgcttttca
    46501 ctcctaccgg ggagcattac ccgcttcttt ttactgttga tcattgtgtt actggtgacg
    46561 atgggggtaa tggtacaaag tgccgttaac gcttggctga aagataaaag ttaccagatt
    46621 gtcgacatta cccacgcaat ccaaaagcgc gtcgatacct ggcgttacgt gaccaggcag
    46681 atctacgaca acattgccgc gacgacctcc ccctcctccg gcgaaggttt acaagagacg
    46741 cgcctgaaac aggatgtcta ctatctggag aaaccacgcc gcaaaacgga agcgttaatc
    46801 tttggctctc acgacaactc aacgcttgag atgactcagc ggatgtccac ttatctggat
    46861 acattgtggg gtgcagaaaa tgtaccgtgg tcgatgtatt acctgaatgg tcaggataac
    46921 agtctggtgc tgatctcaac cctacccctt aaagatctca cctccggatt taaagaatcg
    46981 accgtcagtg acattgttga ttcacgtcgt gcagaaatgt tacaacaggc caacgccctc
    47041 gatgaacgcg aaagtttttc taacatgcgc cgcctagcct ggcagaacgg tcattacttt
    47101 accttgcgta ccacgttcaa ccagccggga catctggcaa cggtcgtggc ttttgatctg
    47161 ccgattaatg atttgatccc accgggtatg ccgctggaca gtttccgcct tgagccagac
    47221 gcgacggcaa cgggaaacaa tgataatgag aaagaaggga cggatagcgt cagtatccac
    47281 tttaacagta cgaagattga aatctcctcg gcactcaact ctaccgatat gcgcctggtc
    47341 tggcaggttc cttatggcac cttattgctg gatacgttgc aaaacattct gctgccactg
    47401 ctgctgaaca tcggtttgct ggcgctggcg ttatttggct ataccacatt ccgccatttc
    47461 tccagccgca gtacagaaag cgtccccagc acggcggtca ataacgaatt gcgcatttta
    47521 cgggcaatca atgaagagat agtctcactg ttgccgctcg gcctgctggt tcacgatcag
    47581 gaatcgaacc gcactgtcat aagtaacaaa attgccgatc atttgctgcc gcatctgaat
    47641 ctgcaaaaca tcaccaccat ggcggaacag catcagggga ttattcaggc gacgatcaat
    47701 aacgagctgt atgagatccg catgttccgc agccaggtcg cgccgcgcac acaaattttc
    47761 attattcgcg atcaggatcg cgaagtgctg gtaaacaaga aactcaagca ggcgcagcgt
    47821 ctgtatgaga aaaaccagca ggggcggatg acctttatga aaaacattgg cgatgcgctg
    47881 aaagaacccg cacagtccct ggcggagagt gcggctaaac tcaacgcccc ggaaagcaaa
    47941 caactggcga atcaggcaga tgtgctggtg cggctggtcg aagaaataca gttagcgaac
    48001 atgcttgcgg atgatagctg gaaaagtgag acggtgctgt tctccgtgca ggatttaatt
    48061 gatgaagttg tgccttcagt gttgcctgcc atcaagcgta aaggtctgca actgctgatt
    48121 aacaatcatc tgaaagcaca cgatatgcgc cgcggcgatc gcgatgcctt acgacgtatt
    48181 ttgctgctac tgatgcaata tgccgtgacc tcaacgcaat tgggaaaaat cacccttgag
    48241 gttgatcagg atgagtcctc tgaagaccgc ctgacattcc gcattctgga cacgggagaa
    48301 ggcgtaagta ttcatgaaat ggataatttg cacttcccgt ttatcaacca gacccaaaac
    48361 gatcgttatg gaaaggcgga cccgctggca ttctggctga gcgatcaact ggcacgtaaa
    48421 ctgggcggtc atttaaacat caaaacgcgg gatgggcttg gtacacgcta ctctgtgcat
    48481 atcaaaatgc tcgccgctga cccggaagtt gaagaggaag aagagcgttt actggatgat
    48541 gtctgcgtaa tggtggatgt tacttcggca gaaattcgga atattgtcac tcgcctgtta
    48601 gaaaattggg gtgcaacctg tatcacaccc gatgaaagat taattagtca agattatgat
    48661 atctttttaa cggataatcc gtctaatctt actgcctctg gcttgctttt aagcgatgat
    48721 gagtctggcg tacgagaaat tgggcctggt caattgtgcg tcaacttcaa tatgagcaac
    48781 gctatgcagg aagcggtctt acaattaatt gaagtgcaac tggcacagga agaggtgaca
    48841 gaatcgcctc tgggcggaga tgaaaatgcg caactccatg ccagcggcta ttatgcgctc
    48901 tttgtagaca cagtaccgga tgatgttaag aggctgtata ctgaagcagc aaccagtgac
    48961 tttgctgcgt tagcccaaac ggctcatcgt cttaaaggcg tatttgccat gctaaatctg
    49021 gtacccggca agcagttatg tgaaacgctg gaacatctga ttcgtgagaa ggatgttcca
    49081 ggaatagaaa aatacatcag cgacattgac agttatgtca agagcttgct gtagcaaggt
    49141 agcctattac atgaacaata tgaacgtaat tattgccgat gaccatccga tagtcttgtt
    49201 cggtattcgc aaatcacttg agcaaattga gtgggtgaat gttgtcggcg aatttgaaga
    49261 ctctacagca ctgatcaaca acctgccgaa actggatgcg catgtgttga ttaccgatct
    49321 ctccatgcct ggcgataagt acggcgatgg cattacctta atcaagtaca tcaagcgcca
    49381 tttcccaagc ctgtcgatca ttgttctgac tatgaacaac aacccggcga ttcttagtgc
    49441 ggtattggat ctggatatcg aagggatcgt gctgaaacaa ggtgcaccga ccgatctgcc
    49501 gaaagctctc gccgcgctgc agaaagggaa gaaatttacc ccggaaagcg tttctcgcct
    49561 gttggaaaaa atcagtgctg gtggttacgg tgacaagcgt ctctcgccaa aagagagtga
    49621 agttctgcgc ctgtttgcgg aaggcttcct agtgaccgag atcgctaaaa agctgaaccg
    49681 cagtattaaa accatcagta gccagaagaa atctgcgatg atgaagctgg gcgtcgagaa
    49741 cgatatcgcc ctgctgaatt atctctcttc tgtgacctta agtccggcag ataaagacta
    49801 atcatctgta ggccagataa gacgcgttag tgtcttatct ggcatttgca ccgattgccg
    49861 gatgcggcgt aaacgcctta tccggcctac gattcccatt atttcaacag attacattaa
    49921 agtaggccag ataagacgcg tcagtgtctt atctggcatt tgcactgaat gccggatgcg
    49981 gcgtaaacgc cttatccgtc ctacgaatcc cgcgatttcc tgaccctctc ggcatatacc
    50041 gtcagcgtct gttttatcac atccagcgtt accggcttcg agaggcagct gtccataccg
    50101 gactccagac accgctgctt ctcttcagcc aacgcattag cagttactcc gattaccggc
    50161 aacgtcagtc ccaactgacg aatgcgttgc gtcaagcggt aaccatccat atttggcatg
    50221 ttgacgtcgc taagcacgat atcaatatga ttcttactaa gtacattaag cgcatcgacg
    50281 ccatcattcg cggttttaca ttgatagccc aacgatccca actgatctgc cagcagacgt
    50341 cggttaatcg gatgatcatc cacgaccaga atcatcatat cgtcgttatc gctgaccgct
    50401 ttgtccgtcg acggcagagc gttagcagga tcgtcgctct ccatctcgat caaataaata
    50461 cgcgccaaca atgccggtag ctcatgcgga gcagccacac tgtgtaccca ctcccctggc
    50521 gctttctcca gcggaatacc aatatggcga cgacagaagg tcactaccgc tctgccctgc
    50581 cattttttac tcactacctc gtcagtgatt aacacatctt cgggagtcgg ttcctgccct
    50641 tcgtatgttg taacgacgat gccgctgcgc tgcaaactgg tttccaggaa ctgacagagc
    50701 gacgcattgc ggaccgccag ccagcagcgt ttaccactca acccttccac gccttttttc
    50761 tgcgggtact gagcgccgta caacggaata cgcacggtaa actggctgcc cattcccggt
    50821 tctgaatcta ccgagatatc gccgtccatc atgctgatca gtttttcaca aatcgccaga
    50881 cccagaccgg tcccctggaa gtttcgctgt acgcccgttc caacctggaa gaagggatca
    50941 aacaagcgca ccacttcttt cgccggaatc cccacgccgg tatcgcgaac acggatagag
    51001 agataatcgc catcagcgcg aacatgcaaa actatacagc cggtatcggt gaattttatg
    51061 gcgttactca acaggttgga gatgacctgc tgtaaacgca tcgggtcgcc atttaaggcc
    51121 actggcacat ccggttcaat aaagcagtac aagcctaact gcttgcgtac caccagcggt
    51181 aaatagttgg cggtgatgtg gttcatcact tcacgcggtg aaaactcacg cggttcgatc
    51241 ttcaactgtt ccgattcaat cttcgagaaa tcgagaatat cgctgataat tttcaacaac
    51301 aggctggaag agttgttcat tgccgtcacc agacgatcga cgcctttcgg taactctttg
    51361 gtttgcaaca gatccaggtt accgataatg ccatacagcg gcgttcgcag ctcatgactg
    51421 acggtggcaa ggaacatcga ttttgactgg ctcgcctgtt ccgctgcttg tgccatctcc
    51481 tgcaacgact cttccatctt cacgcgcgaa gaaacatcca ccagcacaca aatggccacg
    51541 ttttcattac gatagcgcga atggacgaag ctgatttgca gattggtatt gttgctggtc
    51601 aggacatcaa caaaattgac ctgctgccca cagataattt gcgtcagtcg ttggcggtcc
    51661 tcatgcgtaa gcatattgag ataggtatgc gccagttcgt tacttaaaat attgacgcca
    51721 tcagcggtac gcaaaatgca gatacccact ggcgcggagg cgacaatctt gcgattgaac
    51781 tgctcatgtt cttccagtcg cagggcgtcg ctttccgccg gaatgaaaat acgtcgctcg
    51841 tacatccgcg cgagagtaaa caatgcagct ccggcaagca cattcagcaa aattgcgtta
    51901 aggatcaaca tgcgaatgcg ttccagcacc ttatcaaccg gcaccgaata cacgatgctt
    51961 agcgatgagg gtggcagatt tttcttcagc accagctccc ggaacccttc cgtatagcca
    52021 aaccaggagc gttcctgcat ccagcgagga tcgcccttaa ttttactttc tggtccggta
    52081 agcgaaatca gggtatgacc attttcatca agaatggtaa cccccatcgg caacgtaccc
    52141 ggtaagaaaa agttctccat ccggatggtc tgctcgacac ccaaaagcgc ctgcaaccgg
    52201 ttcgccagat aaactggcgt caacgcgtaa aaatacccga cgcaagggcg cggaccttcg
    52261 ctgatccagt agaggttact gccgctatca tcttgtggtg catttcgata tttattgatg
    52321 cgttcatgca acgctttcaa cgcggtatcg cgttccactg gcatatcacg cagaccgaaa
    52381 ttggccatgc agaggttatc actgccgatt aaaaataccc ggttgagatc gtaagccgca
    52441 gaaaaattat cgcgccagta gcgcataaac cacgccaatg actccagaga acctcgccag
    52501 gtgttactca ttgcggaaca atcggagtcg gcaaacagcg gttcaaacgc aggcacatcc
    52561 gcctgcgttt ctcgtccacg cggggaaagc acaccgtttt ctgccgataa gcgattttcg
    52621 gcgatgtact taagctcttt catcacatca gaggtgcgtt gaataaagcg ctgagcctga
    52681 tcggaactca gattaaattc ctgacgaatt tccgattctc gctgatgtaa cgcattaacg
    52741 atgtaaaaaa cggatgaaaa agcaatcaac agccagagca ctaacgccaa tgctctgaac
    52801 atgtagcgcg aggctttcag ggttgtacga aaagaagcaa ggtatttcaa aggggagaag
    52861 ctccgcctca ggtgaccgat ggagtgtggt taaggtagcg gtaaaagcgt gttaccgcaa
    52921 tgttctgtca ccattgcaat aatgcgtatc gcttagaaac cattgcctta accaggaaag
    52981 ccggagtaga tggacagttc tcagtacgaa aaataagaaa tgaccaatct gccgacgctt
    53041 gttaatggtt atatcctggc gtcggcagat cgcccagcag tcttatctga aaaaagagag
    53101 agccaggttg tgtgaaggga tggatcacca gtcaaaactt ttcaccagcg tcagctcgcc
    53161 agcattacgc atcggtacaa taaatgtttc ctgtttctca ttgaccgatc cttcatcggt
    53221 gatcagcgtc agttgggcgg tcgtcaattc cgtttcgctg cgcccaccat agtagttgat
    53281 atacacctga tagcgcccgt gaactggtgc tggcatggcg aaaatctcgg gtccgtaccc
    53341 cgtcgtgaca tccatatcca gtgcaccact gtttttcagc acggtgttac cgtaccaggc
    53401 gtgttcgcca tcgggcgtga caacatggag atcgagatcg gtattgtccg tatcccacga
    53461 gagaaccagc cgtaaacgtg cacgaatagt tcctgtaccc ggcgttgagt aaaactgcat
    53521 tttttgccgg ctttgcccat ccgggctgat gacctgcacg ctgttgctgc cttcagtgaa
    53581 aatataaggg cgtgcaaatg aaccatcgga ttcaattctc tgcggcatac tggcaccgtt
    53641 gaccaccagc cggccctgct gaactttacc cgccgcagca taattcttta ttttgccgcg
    53701 gatctgcgct gagatatttt gatcatctgc catgttgacg gacgacgccg ggtaattgat
    53761 cgattgtgaa aaactggcat cttcgccctc tgccggatgc cagccagaga gcggtgcgtc
    53821 aatctcgact tcctgcacac cttcactgtg agcgacaggc gaaagtgcca ccagtaacaa
    53881 cggaagaaaa atctttcgca tagcattagt ccattaataa ttgtcgggtc agcgtttcga
    53941 tatagttctc atcaagcccg gtgggataac catcaaaggc caagtgcaga tactcatggg
    54001 ttaaatccag ccgatcctga agcgtaaaga agttacgaat aaacagccgt ttctgctgcc
    54061 gatcggtata ggggaaacct gagactaaac gacagaccgc aaacacgtct ggttcgttgt
    54121 accccgtctc gccttgtaat atacgccgcc actgcggcat ttttttcgcc agccaggctt
    54181 tcgctttggg taataactgg caggttgagc gtggagctcc ccagcggcta aggctgttgt
    54241 cgggataagc aaacgcgagt atctggtcgt aacgctcccc ctgccccgcc tgggccgtgg
    54301 cttgccgcca ggaaagtgtc ccttcggtag cgcgactgcc atgataatga accggatcgc
    54361 ctgcatagat gagctcctgc gtccaggcgg tcatcgttcg cgcccccgtg gtcgctggcg
    54421 aagcagaaac acgctgcgtg gcgctgctgt cggggatggt caggcaatca ccttcccgat
    54481 tggcgttttg ctgtaaaaaa gtacgtatcg ccacagtcat ggctttcgct gcctcaggtg
    54541 gcgtactttt cgcctcgcga tccagaactc tggcaacgta ctcttcacga tcgagatgcg
    54601 actgcaattt cagtttgccc ttttcgctca gtaaggtcgt ctcaccgtgg ctgacaaacg
    54661 taatgtggtt accattagta aacgtcacac gatagcgccc attaagtacg ctgggtttta
    54721 ctgccgtcgt actcttttcc gctgtgattt tcttcagcgg atagcgggca aacagctcct
    54781 cctctacaca ttgcccactg gcaacctgcg tcggcactgg caaaacccga ttaagcaccg
    54841 tcgcataacg ggttagtacc gttttgctgg tgccactgcc agtaacccac agcggtgtac
    54901 catcggttaa ccagccagca aaaccaccct gacggatttc ctgtttatca tcggcaaacc
    54961 agctccaggt tttcacccgt acccgactac cgagcatgga tgcgacaccg attttcgcct
    55021 cgtccagcac cacatccagt aagacttcct gagctttgtt ttgcgcaggt aacgtcgcta
    55081 atgaatcaag cagcgattta accgttaccg aggtttctgg cttcagcgtt gtaagagacg
    55141 ctaaccacgc aggtgcctga cgctgttgcc agtattgccc ccacacatct gcgccaatat
    55201 gcaaccgctg cggcgcaaaa tagagcccac aggaacgtac cagcgcggta tcgcgcgtta
    55261 tgctctcccc agcctgacaa caataaacct cttccgggga gtttccgcga cactggtagg
    55321 gctgctccgg ttgatgggta tcctccagcc aggcgtaaac atacagtttc cacaaactcc
    55381 ctaacggcgt ggtgagcgag tcaggtaatg ctgagacttt ggtcacgcct gatgacgaca
    55441 gctgatacag ttgatcgtgc tgcgcaccgc gcagcaccag ttgtaacggc gtctcctccg
    55501 caagcgttgg cagagtaacc aacgccaata gccagacgat tctgcgccag ttcacttatt
    55561 tcacctgcat cccggtccat tcactccctg ccgcgacact ttgctgcgca ggtgcatagg
    55621 aacgcacata acgagcagga ggcagaacga attgcccttt ttgcgagaag cgcagcaaat
    55681 ggcggaaagt gaccgttccc gtcagttctt tcaccggcac catataggcc agttcgccca
    55741 tttcattacg cgctttttcc agcaattgcc cctgctgttt cgcggcgttg ggtttattga
    55801 ctgaaatgcc ccatgttgtg cgctcaacgt cagctcccgg cgggagcggt acttccacct
    55861 gaccatagcg cagaactgca tcctgctcgc tggtaagcgt aatttcatcg agatacagcg
    55921 catcgctgtc aatctcattg ctggtcaccg gctgcagagt aaagctcatc tcttcttcac
    55981 cggggataag ccgatacaac tggcgttcaa cggtcaccgg aatgttactt tgttgagccg
    56041 tttttgccgg ttcacgccag cggacctgca catttgcggc gataattcgt caccaaaaga
    56101 gagaatgtcc ggcacaccct aaccaaccca acgccagtct tcgccccctc cagttaactt
    56161 atgtttagcc catgcgcccg caggcgcagg caaaacaact ggaggcattg tcgccatata
    56221 tttcgccagc cagttcatgg ccagcgcgcg ctcaatagtg gattgctcag cggtcaaacc
    56281 attaaaatag cggccgtctg ggtagcgtca ccaccgctat tgagcagcac aaccgtgtgc
    56341 gccagcggct gattgctgtt tgccgcttta tcccacgcat attgctgaac ctgtggcatc
    56401 accgtggact tcaactgcgc ttttttcagc aacgtcatgg ttaagacgtt tgccaccgca
    56461 tctgccagtg gagattccgg tgtatcgagg atcaggctgt cattgatatc gcggatgtct
    56521 tcctcagaga aatcttcatc tttagttccg cgccgggcga tagcttcatc cccccctttc
    56581 aacaacgttt tgcacggcag attcatctcc tgtgcccacg ccagcaccag cgcccgatgc
    56641 aataacggca tattatccgc ctgctcggca taactgtcga gcatatgctg ccagtattcc
    56701 ggttgttgcg ttacgccgag cgcctggctg gcctgccagt cggcgtacca tgcccatgcc
    56761 gtaaggaagg cgtcaccatt gccatcttca ccccaccagg taaagcgcgc tccgggcccc
    56821 gccagttgca tcagccgcag acggttatcc tgaatcatct gacgaatgtc gttagcggcg
    56881 gcactttgat gatcggcaag cgaacgccag gcgagactga gcgggatcag acggctaccg
    56941 gtgttgatca ccccacccca cggttcatcg actaacgcat caagattgtt gcggaaaatc
    57001 tcctgcggcg tttcactact ttgtagccgg atattactcg cctgctcggg caacatcagc
    57061 gcgttctcgc caccgccgag catgacattt ttctgctgtt caacgggcca gctgttgtcc
    57121 acaaaagaca gttttgtgct aatgctgtcc tgcacttgcc cattttgttg cagttctgca
    57181 cttaacaagc cagattgctg aatattctgc gtcagcgaaa tataattcgc ccctttgtgc
    57241 agcgtcagcg tctggcgcat ctcagcgcct gcaaatttag tcaccagcgc caccggttcg
    57301 ttatcctgct gactgaagat aaacagtcct gccgccggtt tgtcgcccac gcgatacacc
    57361 gttggcatac tccacttcat gtagagattt ttttccgaac gcagataagc acgcccctgc
    57421 ccgaccagcc cgtcgccgtt catcccacgc gcggtgatac gccagcgggt taacgaatca
    57481 ggcatcagga acgtgaaata cgctttgcct tgtttatcgg ttgtgagtga cggcatccat
    57541 gccgcggtat ccacctcttc acgccgtgga cgttcaagca tttttactcg ccgctcgctg
    57601 cggttagtcg cgccaggcgc aaccggctcg ctggagagcg cctggtcata gctgataaac
    57661 gacagactgg agctggtacg cacattgtta cgccccagcg gatagaaaaa tttgccgata
    57721 ttcggcgcga tttctggttg cagcgcgtag atcatttcat cgaccacgcc taccgttagc
    57781 tgcgcagaaa caggtttacc tttcagcgac gaggttaa
//
LOCUS       DAJVQR010000016        55421 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVQR010000016 DAJVQR010000000
VERSION     DAJVQR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(115..1488)
                     /gene="argH"
                     /locus_tag="OQM52_001019"
     CDS             complement(115..1488)
                     /gene="argH"
                     /locus_tag="OQM52_001019"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1562965.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1549..2325)
                     /gene="argB"
                     /locus_tag="OQM52_001020"
     CDS             complement(1549..2325)
                     /gene="argB"
                     /locus_tag="OQM52_001020"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1562966.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2333..3337)
                     /gene="argC"
                     /locus_tag="OQM52_001021"
     CDS             complement(2333..3337)
                     /gene="argC"
                     /locus_tag="OQM52_001021"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1562967.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3491..4642
                     /gene="argE"
                     /locus_tag="OQM52_001022"
     CDS             3491..4642
                     /gene="argE"
                     /locus_tag="OQM52_001022"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1562968.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4994..7645
                     /gene="ppc"
                     /locus_tag="OQM52_001023"
     CDS             4994..7645
                     /gene="ppc"
                     /locus_tag="OQM52_001023"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1562969.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7828..9561
                     /locus_tag="OQM52_001024"
     CDS             7828..9561
                     /locus_tag="OQM52_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1562970.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9710..10561
                     /locus_tag="OQM52_001025"
     CDS             9710..10561
                     /locus_tag="OQM52_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1562971.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10548..10889)
                     /locus_tag="OQM52_001026"
     CDS             complement(10548..10889)
                     /locus_tag="OQM52_001026"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1562972.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10891..11769)
                     /locus_tag="OQM52_001027"
     CDS             complement(10891..11769)
                     /locus_tag="OQM52_001027"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1562973.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11735..14032)
                     /locus_tag="OQM52_001028"
     CDS             complement(11735..14032)
                     /locus_tag="OQM52_001028"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1562974.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14083..14403)
                     /locus_tag="OQM52_001029"
     CDS             complement(14083..14403)
                     /locus_tag="OQM52_001029"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1562975.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14418..15497)
                     /locus_tag="OQM52_001030"
     CDS             complement(14418..15497)
                     /locus_tag="OQM52_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1562976.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15806..16909
                     /locus_tag="OQM52_001031"
     CDS             15806..16909
                     /locus_tag="OQM52_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1562977.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16910..18307
                     /gene="ptsP"
                     /locus_tag="OQM52_001032"
     CDS             16910..18307
                     /gene="ptsP"
                     /locus_tag="OQM52_001032"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1562978.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18319..18981
                     /gene="fsa"
                     /locus_tag="OQM52_001033"
                     /pseudo
     CDS             18319..18981
                     /gene="fsa"
                     /locus_tag="OQM52_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18992..20093
                     /gene="gldA"
                     /locus_tag="OQM52_001034"
                     /pseudo
     CDS             18992..20093
                     /gene="gldA"
                     /locus_tag="OQM52_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20368..20975
                     /locus_tag="OQM52_001035"
                     /pseudo
     CDS             20368..20975
                     /locus_tag="OQM52_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21002..21907)
                     /gene="yijE"
                     /locus_tag="OQM52_001036"
     CDS             complement(21002..21907)
                     /gene="yijE"
                     /locus_tag="OQM52_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1562979.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22001..24181)
                     /gene="katG"
                     /locus_tag="OQM52_001037"
     CDS             complement(22001..24181)
                     /gene="katG"
                     /locus_tag="OQM52_001037"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1562980.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24510..25400)
                     /gene="metF"
                     /locus_tag="OQM52_001038"
     CDS             complement(24510..25400)
                     /gene="metF"
                     /locus_tag="OQM52_001038"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1562981.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25749..28181)
                     /gene="metL"
                     /locus_tag="OQM52_001039"
     CDS             complement(25749..28181)
                     /gene="metL"
                     /locus_tag="OQM52_001039"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1562982.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28184..29344)
                     /gene="metB"
                     /locus_tag="OQM52_001040"
     CDS             complement(28184..29344)
                     /gene="metB"
                     /locus_tag="OQM52_001040"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1562983.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29621..29938
                     /gene="metJ"
                     /locus_tag="OQM52_001041"
     CDS             29621..29938
                     /gene="metJ"
                     /locus_tag="OQM52_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1562984.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30122..30730
                     /locus_tag="OQM52_001042"
     CDS             30122..30730
                     /locus_tag="OQM52_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1562985.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30791..31003)
                     /gene="rpmE"
                     /locus_tag="OQM52_001043"
     CDS             complement(30791..31003)
                     /gene="rpmE"
                     /locus_tag="OQM52_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1562986.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31206..33404
                     /gene="priA"
                     /locus_tag="OQM52_001044"
     CDS             31206..33404
                     /gene="priA"
                     /locus_tag="OQM52_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1562987.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33560..34585
                     /gene="cytR"
                     /locus_tag="OQM52_001045"
     CDS             33560..34585
                     /gene="cytR"
                     /locus_tag="OQM52_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1562988.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34677..35636
                     /gene="ftsN"
                     /locus_tag="OQM52_001046"
     CDS             34677..35636
                     /gene="ftsN"
                     /locus_tag="OQM52_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1562989.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35729..36259
                     /gene="hslV"
                     /locus_tag="OQM52_001047"
     CDS             35729..36259
                     /gene="hslV"
                     /locus_tag="OQM52_001047"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1562990.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36269..37600
                     /gene="hslU"
                     /locus_tag="OQM52_001048"
     CDS             36269..37600
                     /gene="hslU"
                     /locus_tag="OQM52_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1562991.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37667..38593
                     /gene="menA"
                     /locus_tag="OQM52_001049"
     CDS             37667..38593
                     /gene="menA"
                     /locus_tag="OQM52_001049"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1562992.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38686..39171
                     /gene="rraA"
                     /locus_tag="OQM52_001050"
     CDS             38686..39171
                     /gene="rraA"
                     /locus_tag="OQM52_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1562993.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39256..39501)
                     /gene="zapB"
                     /locus_tag="OQM52_001051"
     CDS             complement(39256..39501)
                     /gene="zapB"
                     /locus_tag="OQM52_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1562994.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39927..40772
                     /gene="glpF"
                     /locus_tag="OQM52_001052"
                     /pseudo
     CDS             39927..40772
                     /gene="glpF"
                     /locus_tag="OQM52_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40812..42302
                     /gene="glpK"
                     /locus_tag="OQM52_001053"
     CDS             40812..42302
                     /gene="glpK"
                     /locus_tag="OQM52_001053"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1562995.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42532..43542
                     /gene="glpX"
                     /locus_tag="OQM52_001054"
     CDS             42532..43542
                     /gene="glpX"
                     /locus_tag="OQM52_001054"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1562996.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43639..44385
                     /gene="fpr"
                     /locus_tag="OQM52_001055"
     CDS             43639..44385
                     /gene="fpr"
                     /locus_tag="OQM52_001055"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1562997.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44390..44818)
                     /gene="uspD"
                     /locus_tag="OQM52_001056"
     CDS             complement(44390..44818)
                     /gene="uspD"
                     /locus_tag="OQM52_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1562998.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44845..45144)
                     /locus_tag="OQM52_001057"
     CDS             complement(44845..45144)
                     /locus_tag="OQM52_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1562999.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45356..45796)
                     /locus_tag="OQM52_001058"
                     /pseudo
     CDS             complement(45356..45796)
                     /locus_tag="OQM52_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45897..46496
                     /locus_tag="OQM52_001059"
     CDS             45897..46496
                     /locus_tag="OQM52_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1563000.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46604..47371
                     /gene="tpiA"
                     /locus_tag="OQM52_001060"
     CDS             46604..47371
                     /gene="tpiA"
                     /locus_tag="OQM52_001060"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1563001.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47426..48181)
                     /gene="cdh"
                     /locus_tag="OQM52_001061"
     CDS             complement(47426..48181)
                     /gene="cdh"
                     /locus_tag="OQM52_001061"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1563002.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48288..49277)
                     /gene="sbp"
                     /locus_tag="OQM52_001062"
     CDS             complement(48288..49277)
                     /gene="sbp"
                     /locus_tag="OQM52_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1563003.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49597..50559)
                     /gene="pfkA"
                     /locus_tag="OQM52_001063"
     CDS             complement(49597..50559)
                     /gene="pfkA"
                     /locus_tag="OQM52_001063"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1563004.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50740..51642)
                     /gene="fieF"
                     /locus_tag="OQM52_001064"
     CDS             complement(50740..51642)
                     /gene="fieF"
                     /locus_tag="OQM52_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1563005.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51790..52290)
                     /gene="cpxP"
                     /locus_tag="OQM52_001065"
     CDS             complement(51790..52290)
                     /gene="cpxP"
                     /locus_tag="OQM52_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1563006.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52440..53138
                     /gene="cpxR"
                     /locus_tag="OQM52_001066"
     CDS             52440..53138
                     /gene="cpxR"
                     /locus_tag="OQM52_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1563007.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53135..54508
                     /gene="cpxA"
                     /locus_tag="OQM52_001067"
     CDS             53135..54508
                     /gene="cpxA"
                     /locus_tag="OQM52_001067"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1563008.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54614..55288)
                     /gene="yiiM"
                     /locus_tag="OQM52_001068"
     CDS             complement(54614..55288)
                     /gene="yiiM"
                     /locus_tag="OQM52_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1563009.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13494 a        15018 c        14165 g        12744 t
ORIGIN      
        1 cagaatgctg acaaatttct gcctgatgcg gcgtaaacgc cttatccgcc tacgaagtgt
       61 cgcactttgt aggcgtggta aactccccat tttcagggga gcaatataaa caaattactc
      121 taaccgagcc tgcgcaaaag caatcgcctg cgccacctgc tgcggtgaga cgccgccttt
      181 tgccgcacgc ttttcgaggc acgattgtag cgacagaatc ggatagacat cttcgccaat
      241 cacctgactg aatttctgca actcactgag tggcagatct tccagcggtt tgccctgacg
      301 aatggcttcc accaccgctt cacccacaat atggtgcgcc tcgcggaaag gtacgccttt
      361 cgccaccagg taatccgcca gttcagtggc gttcgcatac ccctgctgcg ccgcttcctg
      421 acaacgcgga cgtttcacct gaatgccgtc cagtaccagc gccgccatat gcaggcagtc
      481 cagccaggta tcgagcgcgt cgaacagacc ttctttgtct tcctgcatat ctttgttgta
      541 agccagcggc aaacctttca gcgtcatcat catgccggtt aacgcaccct gcacccggcc
      601 gcatttaccg cgaatcagct ccagcgcatc cgggtttttc ttctgcggca ttaatgatga
      661 accggaagtc acgcggtccg aaagctccac aaaccccgct tcgccggtgt taaagaaaat
      721 cagatcttca gcaaaacgcg acagatgcac catgccgata gcggcagcag aaagcagttc
      781 caacacatgg tcacggtcag aaacgctgtc cagactgtta cgggtcgccg aagcaaagcc
      841 cagccagcct gctaactgtt cacggtcgat ttcataggcc gttcccgcca gcgcgccaca
      901 gcctaatggg ctgacatcca gacgcttaag cgcatcctgc aaacggcttt catcacgcgc
      961 cagcatctca acataggcca ggcaccagtg cgcgaacgtc accggctggg cgcgttgcag
     1021 gtgagtgtaa cctggcatta ccgcgtcctg attgttttgt gcggtttcca ccagcgccga
     1081 ttgcagctgc cggttagccg tcagtaactc gctaacggta tctttgcacc acagtttcag
     1141 gtcagtcgct acctgatcat tacggctacg cccggtatgc agctttttgc ctaactggcc
     1201 cactttgtcg atcagtttgc cttccaccca gctatggata tcttcggcgt cgctttcaag
     1261 gatttgttgt ggcctggcgc gaacatcttc cagcaacacg ttcagcgcct cttccagttg
     1321 cgcctgctct tctgcggtta acacgccgac cgttaccaag gctttggacc aggccacaga
     1381 gccaacaata tcctgctccg ccagacggta atcaaagcgc agtgagtcgt tgaattgttt
     1441 gaaccgttga tctgctgcct gggtaaaacg cccgccccaa agtgccataa ctctgtttcc
     1501 ttattttgaa attcaatgcc ggaaagctgc gcctccggct aacaaaactt aagctaaaat
     1561 ccgcgtaccc atcggcatac cgttaaacag tgccggaagc tgctccgcat aacgccaaga
     1621 agcgatatct accggacggc ccagcgtgcg ggccgcatcc agcgccgcgt tcactttcac
     1681 tatcatgccg tcagtaataa tgccctgctc aatcagttgt tctgctttcg cggcggtcat
     1741 ttcggcaatg cgttgccctt tgccgtcgag aatgccgctg acgtcggaga gcaaaatcag
     1801 atccgcgccc agcgttgccg ccagcgccgt tgccgcctgg tcggcattga cgttcatcag
     1861 ttgcccttcg tctgttacgc caatggagct gaccaccggc agataaccgt tctccagcaa
     1921 ggagttgata agcttaggcg aacctggctg cgccagtcca acatgaccta actcttcatc
     1981 aagctgggtc actttgacgc tgtcgccgtc accgagaaac aaacctacgg ccgcaatctg
     2041 atgtttcttc gcccatgcca acagggtttt atttgccgtt cccgccagtg ctccggtgat
     2101 aatgtctatc tgatcagcag gcgtcacccg caggccgttt ttctttttca ccggcagatt
     2161 cagccctttc atcagctcat ccaccacgca accgccgccg tgcacaatca ccagcggacg
     2221 ctgatgtgac tcacgataat tcaccaatgc gctaaacaga cgttccagcg cctcttcact
     2281 atccagcagt acgccgccca gtttgataat taatggattc atcattgcac ccttaaataa
     2341 gagactgcgt ttccgcatag ccgaaacgaa tattggcgca ctgtaccgct tgtgccgccg
     2401 cgcctttcag taagttgtct tcggtcgcca caataatcag atgctcgccc tgaacggcaa
     2461 acccgatatc gcaaaatggc agcccaacga catttttcag cgccggaacg cctttgtcat
     2521 acagccgcac cagcggttta tgggcatacg cctgctgtaa cgcttgcgcg acttgcgcct
     2581 gggtcacacc cgatttcagg cggcaggtaa tggtttcgag aatgccgcgc gggaaattgc
     2641 ccagatgtgg ggtgaagata acgtcagcac cgaggtgtgt ggcgatctct ggttgatggc
     2701 gatgagtaaa gacgccatac ggttgcaggc taacttcaca aaaactattt gaaatcgctg
     2761 ccttacgtcc tgcaccgctc acgccgctgg tggcgttgat caccggccac tgattgaggt
     2821 caagaagatc ggcatcaatc aacggtttca gcgccagctg tgccgccgtc ggataacagc
     2881 ctggcaccgc aatcaaattc gcttctttta atttattacc gcaccactcc gccagaccgt
     2941 aggctgcctg ttccagcagt tccgggtatt gatgggtaaa gccgtaatat ttttcataga
     3001 aggtggcgtc gttaacacga aacgcgccgg aaaggtcgaa caccacgcag cccgcttcaa
     3061 gaaactgcgg cgctaaatcg tggctaactt catgggcggt ggcgagaaac actacgtcca
     3121 cccctgggct aaactcgctg atatccgaca tcggctgcaa cggcagctca acgatgcctt
     3181 ttagctgcgg atgcaaatcg gagattaact ttcccgcatc attgctttgc gctgaaacag
     3241 tcaaagcggt tatgttcata tgcggatggc gatttacata ggtcactagc tctgcgccag
     3301 cgtagccgct ggcacccaca atcagcgtat tcaacatcgg ggctattcac cttcttatgt
     3361 ctggttgcca ggttaaacgt aaaacattca ccttacggct ggtgggtttt attacgctca
     3421 acgttagtgt atttttattc ataaatactg catgaatatt gatactatca tgaccagagg
     3481 tgtgtcaaca atgaaaaaca aattaccgcc atttatcgag atttaccgcg ctctgattgc
     3541 cacaccttca ataagcgcta cggaagaggc actcgatcaa agcaatgcag atttaatcac
     3601 tctgctggcg gactggttta aagatttggg cttcaatgtg gaagtgcagc ctgttccagg
     3661 aactcgcaac aaatttaata tgctggccag ttacggacag ggggctggcg gcttgttgct
     3721 ggcggggcat accgatacgg tgccatttga tgacggtcgc tggacgcgcg atccgtttac
     3781 actgacggag catgacggca agctttacgg cttaggcacc gccgacatga aaggcttttt
     3841 tgcgtttatc cttgatgcgc tacgcgatgt cgacgtcacg aaactgaaaa aaccgctcta
     3901 cattctggcg actgctgatg aagaaaccag tatggccgga gcgcgttatt ttgccgaaac
     3961 taccgccctg cgcccggatt gcgccatcat tggcgaaccg acgtcactac aaccggtacg
     4021 cgcacataaa ggtcatatct ctaacgccat ccgtattcag ggccagtcgg ggcactccag
     4081 cgatccagca cgcggaatta acgctatcga actaatgcac gacgccatcg ggcatatttt
     4141 gcaattgcgc gataacctga aagaacgtta tcactacgaa gcatttaccg tgccataccc
     4201 tacgctcaac ctcgggcata ttcacggtgg cgacgcttct aaccgtattt gcgcttgctg
     4261 tgagttgcat atggatattc gtccgcagcc tggcatgaca ctcaatgaac ttaatggttt
     4321 gctcaacgat gcattggctc cggtgagcga acgctggccg ggtcgtctga cggtcgacga
     4381 gctgcatccg ccgatccctg gctatgaatg cccaccgaat catcaactgg ttgaagtggt
     4441 tgagaaattg ctcggagcaa aaaccgaagt ggtgaattac tgtaccgaag cgccgtttat
     4501 tcaaacgtta tgcccgacgc tggtgctcgg tccgggttcc atcaatcaag ctcatcagcc
     4561 tgatgaatat ctggagacgc ggtttattaa acccacccgt gagttgatta tccaggtaat
     4621 tcaccatttt tgctggcatt aaaacgtagg ccggataagg cgctcgcgcc gcatccggca
     4681 ccgttgccaa actccggtgc cgcaataatg tcggatgcga tgcttgcgca tcttatccga
     4741 cctacacctt tggtgttatg tatggcgatt ttttaacatt tccataagtt acgcttattt
     4801 aaagcgtcgt gaatttaatg gcgtaaattc ctgctattta ttcgtttgct gaagcgattt
     4861 cgcagcattt gacgtcaccg cttttacgtg gctttataaa agacgacgaa aagcaaagcc
     4921 cgagcatatt cgcgccaatg cgacgtgaag gatacagggc tatcaaacga taagatgggg
     4981 tgtctggggt aatatgaacg aacaatattc cgcattgcgt agtaatgtca gtatgctcgg
     5041 caaagtgctg ggagaaacca tcaaggatgc gttgggagaa cacattcttg aacgcgtaga
     5101 aactatccgt aagttgtcga aatcttcacg cgctggcaat gatgctaacc gccaggagtt
     5161 gctcaccacc ttacaaaatt tgtcgaacga cgagctgctg cccgttgcgc gtgcgtttag
     5221 tcagttcctg aacctggcca acaccgccga gcaataccac agcatttcgc cgaaaggcga
     5281 agctgccagc aacccggaag tgatcgcccg caccctgcgt aaactgaaaa accagccgga
     5341 actgagcgaa gacaccatca aaaaagcagt ggaatcgctg tcgctggaac tggtcctcac
     5401 ggctcaccca accgaaatta cccgtcgtac actgatccac aaaatggtgg aagtgaacgc
     5461 ctgtttaaaa cagctcgata acaaagatat cgctgactac gaacgcaacc agctgatgcg
     5521 tcgcctgcgc cagttgatcg cccagtcatg gcataccgat gaaatccgta agctgcgtcc
     5581 aagcccggta gatgaagcca aatggggctt tgccgtagta gaaaacagcc tgtggcaagg
     5641 cgtaccgaat tacctgcgcg aactgaacga acaactggaa gagaatctcg gctacaaact
     5701 gcccgtcgaa tttgttccgg tacgttttac ctcgtggatg ggcggtgacc gcgacggcaa
     5761 cccgaacgtc actgccgata tcacccgcca cgtcctgctg ctcagccgct ggaaagccac
     5821 cgatctgttc ctgaaagata ttcaggttct ggtttctgaa ctgtcgatgg ttgaagcgac
     5881 ccctgaactg ctggcgctgg ttggcgaaga aggtgccgca gaaccgtatc gctatctgat
     5941 gaaaaacctg cgttctcgcc tgatggcgac acaggcatgg ctggaagcgc gcctgaaagg
     6001 cgaagaactg ccaaaaccag aagggctgct gacacaaaac gaagaactgt gggaaccgct
     6061 ctacgcttgc taccagtcac ttcaggcgtg tggcatgggt attatcgcca acggcgatct
     6121 gctcgacacc ctgcgccgcg tgaaatgttt cggcgtaccg ctggtccgta ttgatatccg
     6181 tcaggagagc acccgccata ccgaagcgct gggcgagctg acccgctacc tcggtatcgg
     6241 cgactacgaa agctggtcag aggccgacaa acaggcgttc ctgatccgcg aactgaactc
     6301 caaacgtccg cttctgccgc gcaactggca accaagcgcc gaaacgcgcg aagtgctcga
     6361 tacctgccag gtgattgccg aagcgccgca aggctccatt gccgcctacg tgatctcgat
     6421 ggcgaaaacg ccgtccgacg tactggctgt ccacctgctg ctgaaagaag cgggtatcgg
     6481 gtttgcgatg ccggttgctc cgctgtttga aaccctcgat gacctgaaca acgccaacga
     6541 tgtcatgacc cagctgctca atatcgactg gtatcgcggc ctgattcagg gcaaacagat
     6601 ggtgatgatt ggctattccg actcagcaaa agatgcgggc gtgatggcag cttcctgggc
     6661 gcaatatcag gcacaggatg cattaatcaa aacctgcgaa aaagcgggta ttgagctgac
     6721 gttgttccac ggtcgcggcg gttccattgg tcgcggcggc gcacctgccc atgcggcgct
     6781 gctgtcacaa ccgccaggaa gcctgaaagg tggcctgcgc gtgactgaac agggcgagat
     6841 gatccgcttt aaatatggtc tgccagaaat caccgtcagc agcctgtcgc tttacaccgg
     6901 ggcgattctg gaagccaacc tgctgccacc acctgagccg aaagagagct ggcgtcgcat
     6961 tatggatgaa ctgtcagtca tctcctgcga tctctaccgt ggctacgtac gtgaaaacaa
     7021 agattttgtg ccttacttcc gctccgctac gccggaacaa gaattgggca aactgccgtt
     7081 gggttcacgt ccggcgaaac gtcgcccaac cggcggcgtc gagtcgctgc gcgccatacc
     7141 gtggattttt gcctggacgc aaaaccgcct gatgctcccc gcctggctgg gtgcaggtac
     7201 ggcgctgcaa aaagtggtcg aagatggcaa acagagcgag ctggaagcca tgtgccgcga
     7261 ttggccattc ttctcgacgc gtctcggcat gctggagatg gtcttcgcca aagcagacct
     7321 gtggttggcg gaatactatg atcaacgtct ggtagacaaa gcactgtggc cgttaggtaa
     7381 agagttacgc aacctgcaag aagaagacat caaagtggtg ctggcgattg ccaacgattc
     7441 ccatctgatg gccgatctgc cgtggattgc agagtctatt cagctacgga atatttacac
     7501 cgacccgctg aacgtattgc aggccgagtt gctgcaccgc tcccgccagg cagaaaaaga
     7561 aggccaggaa ccggatcctc gcgtcgaaca agcgttaatg gtcactattg ccgggattgc
     7621 ggcaggtatg cgtaataccg gctaatcttc ctcttctgca aaccctcgtg cttttgcgcg
     7681 agggttttct gaaatacttc tgttctaaca ccctcgtttt caatatattt ctgtctgcat
     7741 tttattcaaa ttctgaatat accttcagat atccttaagg aattgtcgtt atattcggcg
     7801 atattttttt caagacaggt tcttactatg cattccacag aagtccaggc taaacccctt
     7861 tctagctgga aagccctggg ttgggcactg ctctactttt ggtttttctc tactctgcta
     7921 caggccatta tttacatcag tggttatagt ggcaccaacg gcattcgcga ctcgctgtta
     7981 ttcagctcgc tgtggttgat cccggtattc ctctttccga agcggattaa aattattgcc
     8041 gcagtgatcg gcgtggtgct atggacggcc tctctggcgg cgctgtgcta ctacgtcatc
     8101 tacgggcagg agttctcgca gagcgttctg tttgtgatgt tcgaaaccaa caccaacgaa
     8161 gccagcgagt atttaagcca gtatttcagc ctgaaaattg tgcttatcgc gctggcctat
     8221 acggcggtgg cagttctgct gtggacacgc ctgcgcccgg tctatattcc aaagccgtgg
     8281 cgttatgttg tctcttttgc cctgctttat ggcttgattc tgcatccgat cgccatgaat
     8341 acgtttatca aaaacaagcc gtttgagaaa acgttggata acctagcctc gcgtatggag
     8401 cctgccgcac cgtggcaatt cctgaccggc tattatcagt atcgtcagca actaaactcg
     8461 ctaacaaagt tactgaatga aaataatgcc ttgccgccac tggctaattt caaagatgaa
     8521 tcgggtaacg aaccgcgcac tttagtgctg gtgattggcg agtcgaccca gcgcggacgc
     8581 atgagtctgt acggttatcc gcgtgaaacc acgccggagc tggatgcgct gcataaaacc
     8641 gatccgaatc tgaccgtgtt taataacgta gttacgtctc gtccgtacac cattgaaatc
     8701 ctgcaacagg cgctgacctt tgccaatgaa aagaacccgg atctgtatct gacgcagccg
     8761 tcgctgatga acatgatgaa acaggcgggt tataaaacct tctggatcac caaccagcag
     8821 acgatgaccg cccgcaatac catgctgacg gtattttcgc gccagaccga caagcagtac
     8881 tacatgaacc agcaacgtac gcagagtgcg cgtgaatacg acaccaacgt gctgaagccg
     8941 ttccaggatg tgctgaatga ccctgcgccg aagaaactga tcatcgttca tctgctgggt
     9001 acgcatatca aatacaaata ccgctacccg gaaaatcagg gcaagtttga tggcaatacc
     9061 gatcatgttc cgccaggatt aagcgcagaa gagctggaat catataacga ttatgacaac
     9121 gctaacctgt ataacgatca tgtggttgcc agcctgatta aagactttaa agcggcagac
     9181 ccgaacggat tcctggttta cttctctgac cacggtgaag aggtttacga cacgccgccg
     9241 cacaaaactc aggggcgtaa cgaagacaac ccgacgcgcc acatgtacac cattccgttc
     9301 ctgctgtgga cgtcggaaaa atggcaagcg actcatcccc gtgatttctc acaggatgtc
     9361 gatcgtaaat acagcctggc ggaactgatc cacacctggt cagatttggc gggcttatct
     9421 tacgacggtt acgatccaac ccgttcagtg gtgaatccgc agttcaaaga aactacccgc
     9481 tggattggta acccgtacaa gaaaaacgcg ctgatcgatt acgacactct gccgtatggc
     9541 gatcaggtgg gtaatcagta gatgaccttt gtatcgcgtc tggcgctatt tgccggatgc
     9601 gatactgccg caatgtttac atcgaactcc cctcaggcca cacttttcag gtggcctttt
     9661 ttaataatgc cagttactgt tttcccgtct acatacctaa gggaataaca tgtatcacga
     9721 cgtcagctat ctgctctccc gcctgatcaa cggcccgctg tccctgcgcc agatttactt
     9781 tgccagcagt aacggcccgg tgcccgatct tgcgtatcag gtcgattttc cacggctgga
     9841 aattgtgctg gaaggtgagt ttgttgatac cggcgctgga gcaacgttag ttcccggcga
     9901 tgtgctgtac gttgccgctg gtggctggaa tttcccacaa tggaaaacac ccgctaccac
     9961 ctttagcgtg ctgtttggca aacagcaact cggcttcagc gtcgtgcaat gggatggcaa
    10021 acaatatcaa aatctggcga agcaacacgt cgcccgacgc ggcccacgca taggttcttt
    10081 tctgctacaa acgctcaatg aaatgcaaat gcagccgcag gagcagcaaa cggcaaggct
    10141 tatcgtagcc agcctgctta gccactgccg cgatttgctc ggcagccaaa tccagaccgc
    10201 ctcacgcagc caggcactat ttgaagctat tcgcgattat atcgacgaac gctatgcctc
    10261 cgcgcttacc cgcgaatctg tcgcacaggc attctatatt tcaccaaact atctctcgca
    10321 cctgtttcaa aaaacggggg ccattggttt taacgaatac ctgaatcaca cgcgactgga
    10381 gcacgctaag acgttattga aaggctatga cctgaaggta aaagaagtgg cgcacgcctg
    10441 cggctttgtc gacagcaact acttctgccg tctgtttcgt aaaaacaccg aacactcgcc
    10501 gtcggaatat cgccggcagt accacagcca actgacagaa aaaccgacta ctccagaata
    10561 agatgggttt gctgcggcgc agaaagcact ttgcgcaccg cgctcattac ccgctgcggc
    10621 tcacgcagga atgcgtagat gctgcattgc acatagcggc aatgttcaaa tcgctcggca
    10681 ccggcaagct cgatatccgt aatcaacaga gcaacatccg cccgacggat atcctcgtcc
    10741 gctaaacgat tctccgttcc cagcgcgccc tgagtttcaa tgctgacgcc ccacttctct
    10801 aactggcaca acttttccag ccgttccgcc gccatataag tatgcgccac gccgcttacg
    10861 caggcggtta ctgccaccag gtatgccatt ttaacctccc acggtaacct gaaatccggc
    10921 ccgttctgcc atttcgcgca tcgttgtcac atcggctgac gacggcgcag gcacctcttt
    10981 catcgaccat gttttcccca gcaggcggta tttcggttcg ccgtactgat gaaacggtaa
    11041 cagatggatc tgcctgattt tcagcgggat cagtacatcc agcgcctgct gcatattctc
    11101 ccggctgagc gtgaaaccag ggatcaacgg taaacgcggg atcacgttga caccctcact
    11161 caccagcaaa cgcagattct ccagcacgcg tggcaggttc atcttcacca catcccgcgc
    11221 ctgagtcgcg tccataattt ttaaatcgaa cagcacttca tcgcacaatt tcgccagcgg
    11281 taacagcttg cttgctggcg cgtctccggc agtttcaatg gcgcatgaga caccccacag
    11341 ccgcagtcgc tgtaaaaaac gggtagcaaa ctccgcctgc attaacactt cgccgccaga
    11401 aagcgtgacg ccgccgccgg acgtgcgaaa gaaaatgtca tctttcatca cttcccgttc
    11461 cagagcgtca aggctgatat cgcgaccaat ccgttcaaac gccccggagg ggcattcatc
    11521 cgcatcacgc aaacatttcg cacagtgcag acatttcgcc tctctgcgta ccgtctggat
    11581 tttgccggag atcgactccg gattagcaca ccacgggcaa agatgcggac agcctttaaa
    11641 aaagaccacc gtacgaatgc cctcgccgtc attcagtgaa taacgctgaa tgttgaaaat
    11701 gcgcgccaca tcatcgcggc gcgtttccac aacgttacag ctgatgcgct gtccggcgga
    11761 tgatgtcatc ctggatctcc ttcgacagtt cgacaaagaa ggcgctgtat ccggcaacgc
    11821 gcaccaccag cccggcataa tcttgtgggc gctgttgcgc ttcccgcaac gtgtcggcgt
    11881 tcaccacgtt aaactgaata tgctgcaact taagctgggt aaacacccgt aagaagtcgg
    11941 ccagtttgcg taatcccgct tcaccttcca gggtcgccgg agtgaatttc acgttcagca
    12001 acgtaccgtt agacagcagc gtattatcga gcttactgac tgacttcagt accgccgttg
    12061 gcccttgtgc gtcctgaccc agcataggtg acaagccgcc gtctgccagt tgttccccgg
    12121 caaaacgacc gtctggcgtc gcgccaacca ccgatcccaa cggaacgtga gcagaaacgg
    12181 tatacgatcc cggcgtgaag taaccgccgc gcgggttctg gtatttttcc acttctttgc
    12241 agtagtggcg caacagttcg gcgctaatgt tatccacctc gtcgatatcg ttaccgtatt
    12301 tctcaaagcg gttaattaag cgagcgcgga ctttttcgcc ttctggcgtt gcaaagttgg
    12361 cttttaatac cgacagcaat tcgtcaaaac ttaaacgctg ttgctcaaaa accatccctt
    12421 tcaacgcatg gagagaatcg ctcaggttgg cgataccaat tccctgtacg ccggagaagt
    12481 tataacgcgc gccgccatcg gtaatatcgc gacctttttc cagacaatcg ctgataaaag
    12541 acgagagcag cggtacaggt gcccagtcgc gatggccgat atcacaaata ttgctgccct
    12601 caaccatcag ggtgatgtag tggctgatct tggcgcggat ctgctccagt aaaccttcat
    12661 aagtcagcgc agcattgcct tcattttcat gcaggcagat ttccatcact ttcagcagat
    12721 taaacatcgc gatgtcatgc aagccgtagg ttctgccggg aatagataat tccacacagc
    12781 ccactacgga atagtcgcgc gcatcttcca gcgaaacgcc acggttgagg aacgctggca
    12841 ccaccacttc atcgttaaag atttgcggaa taccggtgcc gaggcgaatg gtttcggcgg
    12901 ttttcatcag gaacggcgtg tcgatcaagg cgttagtgcg cacgccgagg ttcggttgcg
    12961 gtaattgcac gctttgatag gcgtcaaggc aaaggaacga aagcacgttc accgcgctac
    13021 gtccgttctc ggttaacccg ccgagcagtg cggtatagcc ggtcgggaaa ccggcgaaat
    13081 aacgcgcgct actggtggag cgcaacagca cgatgtcgtt gcatttcacc cataaagatt
    13141 cgagcagttc tttcaggaac gccggatctt cgccctgggt taaagatgcc tgatagaacg
    13201 gcaacatata ctggtcgaag cgccctaaag acagcgaact ggcgttggat tcgtattgca
    13261 gaatgatgtt catgtaccag aataactggc acgcctgcca gaacgtctgc ggcttatgtt
    13321 gcgcgttatg gcgggagatc tccgcaatag tcagcagctc ttcgcgacgc tgggcatctg
    13381 tgcagtttgc cgccatcgtt tccgccagtt cggcgtaacg caaaatgtgt ttctgcgagg
    13441 cttccagcag taacaacgcg gcctgataaa agtgattctc cggctgttgc tgacaatgtt
    13501 gctgcatctg tgccaccagc tcacccagac cgtgattcaa caggcgtggg taatcaataa
    13561 taatgtgccc ctggccttta tccgtctggt tgatgctgaa aatctgcgtg ttggtcgcgg
    13621 cttttacttc atctgtcatc tgcccgttga tgaaatcttt catcgaacgt ttttcccagt
    13681 acgggaacaa ctcttcacgg tagatacgtt tatcttcttc gctgatagca aagcggtcct
    13741 gcggacgcgt cgggaattga tccagctctt tcagcagcca gtaagggtcc atttccggcg
    13801 acataatccc ggcgcgcggt tttacggtgc ggttaccggc aatcagttct tcatcacgaa
    13861 tcgaaatttc aacatgttca aggatatacg ctgttgcttt cgcccggcgc aatatcaccg
    13921 gttcgccttc ggtttgccga tggctggcgg tataaagcag cgcccgctcc agcgagattt
    13981 cacgggtatt ggcaaacagt gcagttttga ggcgagagat acgattcgtc atgaggaact
    14041 cctgtgggaa agcgaacccg cccttgcgag cgggagaaga acttaagcgg tttgcgccag
    14101 gtgagcttca attttgctca taatggcgtc agcacgtttt actgcatcgc tgatgttgac
    14161 gcgcacgatg gtcttgccag caaaacgctc ttcaaacttg atgccgatat ctttggtcag
    14221 aatcaccata tcagcgctcg ccacgtcttc cgcagtcagt tcattttcca gaccaattga
    14281 cccctgggtt tctactttca cttcccagcc tttcgctttc gcggcacttt ccagcgcctc
    14341 tgctgccata taggtatggg caacacctga aggacatgcg gttactgcaa taattttcgt
    14401 catggctcgt tcctcaatta attaatttca aaatccaaat ccaggtcgtc ttctttttca
    14461 tcagtcgaag acccattttt acgcgccaga cttttcagca cgttcacaca aacagccgtc
    14521 accactgctc cgacagccac tgctgcgata taacccaact taccttcaac caccggcagc
    14581 acaatcagac caccccagcc tgcatagcac tgcgcaccga ccagcgccgc agttactgca
    14641 ccacaaactg aaccgaccat aatggaagga atgacacgca gcggatcggc agcggcgaaa
    14701 ggaatcgccc cttcagtaac gccaacacag cccatcacca gtgccgcttt gccggtttcg
    14761 cgctcttctg cggagaaatt tttacgacca atcagcgtcg ccagccccat ccccagcggc
    14821 gggatacaaa tacccacggc agcaatggcc accacggtat aaacaccctg agcaacgcaa
    14881 atcagcatga aggcataggc cactttgtta accggaccgc ccatatcgaa cgccagcatc
    14941 agccccatga tcaccgccag cataacaatg ctgccctgct gcatcccctg aagccactga
    15001 gtcaggctgt tggtcaacgc ccctacaggc tcgcccaagc cccacatcat gatgcctgcg
    15061 gtaatcagtg tgccgatgat aggaataatg aagatgggca tcaccgagcg cagaacttta
    15121 tgcaccggaa ttttcttcag gtaatgcacc acgatgccgc cgataatccc ggcgatcagc
    15181 gcaccaaaga agcccgcacc aaagctgtta ccaacccagg caccgatagc gcacggagcc
    15241 agcgcagaac gctctgcaat ggagtaacca atgtaagcgg cgaggaaagg caccatcagc
    15301 gtcaaacctg caacgccgat atcaaacagt tttttcaaat tcggatcggc tacggcatcc
    15361 ggcactgcgc ctttgccata caacatgacg gaaaccgcca gcaaaatacc gcccgatacc
    15421 acgaagggaa tcatgtgtga aacgcccgtc attaaatgct gacgggtatt tttcaggatc
    15481 tgcaccaact cattcataag ctgctcccgg gcttgcaaaa gcccatgtcc atatttttga
    15541 tggaggaaac aataggcaat cgcaggcgga gcaaacagtg gataaaaagg ccatttactg
    15601 gacttttgta atatcagtac aaaaatgcga tccgcctcat aacttgcgat aatccggaag
    15661 catgccgcaa aacggcgtct tgtgagacgc gtcgcaaatt gacggttaca ttacatttgt
    15721 ccagcttttg gcagatttgt cacatggcag gccgttaccg tgcgctctta tcctgtaaac
    15781 agaaaaccgt accgggagaa aggcaatggc cctgattgtg gaatttattt gtgagctacc
    15841 taacggcgta catgcgcgtc cggcaagcca cgttgaaacg ctgtgtaata ctttttcatc
    15901 tcaaattgag tggcataacc tgcgtactga ccgtaaaggc aacgccaaaa gcgctctggc
    15961 gctgattggc accgatacgc tggtgggtga taactgccag ctactgattt ccggggccga
    16021 cgaacaggaa gggcaccagc gtttaagcca atggctgcgc gatgaattcc cccactgcga
    16081 tgcgccgctg gcggaagtta aatctgacga actggaacca ctgccggttt cactgaccaa
    16141 tctgaatccg caaattatcc gcgcccgcac cgtgtgcagc ggtagtgcag gcggcattct
    16201 gacgccgatc tcttctttag atctcaatgc gctgagtaat cttcccgcag ccaaagacgt
    16261 tgacgccgag caatccgcac tggaaaacgg cctgacgctg gtactgaaaa acattgagtt
    16321 tcgtctgctg gatagcgacg gtgctaccag cgcgattctg gaagcgcacc gatccctggc
    16381 tggcgatact tccctgcacg aacatttact ggcaggcgtc agcgccggat taagctgcgc
    16441 cgaagcgatt gtcgccagtg cgaatcactt ttgcgaagag tttgcccgtt ccagcagcag
    16501 atacctgcaa gaacgtgccc tggacgtacg cgacgtctgc ttccagttac tccagcaaat
    16561 ctacggtgag caacgcttcc cggcaccggg caaactgacg cagtccgcca tttgtatggc
    16621 tgatgaactg acccccagcc agttcctcga actggataaa aatcacctca aaggattgtt
    16681 actgaaaagc ggcggcacca cctcacatac ggtgatcctt gcccgttcgt tcaacattcc
    16741 gacgctggtt ggtgtggata ttgatgccct cactccgtgg cagcatcaaa cgatttatat
    16801 cgacggcaac gccggggcga ttgtggttga gccaggagaa gccgtagctc gttattatca
    16861 gcaagaagcc cgcgtacagg acgccctgcg tgagcaacag cgtgtctagc tgacccaaca
    16921 agcccgtacc gctgacggta tccgcattga aattgccgct aacatcgctc actccgtgga
    16981 agcgcaggcc gcattcggca atggtgcgga aggcgttggt ttgttccgca ctgaaatgct
    17041 ctatatggat cgcaccagtg caccgggcga aagcgagctg tacaacattt tttgtcaggc
    17101 gctggagtct gccaacggac gcagcattat tgtgcgcact atggacattg gcggcgacaa
    17161 acccgttgat tatctaaaca ttcccgcaga ggcaaacccg ttcctcggtt atcgcgccgt
    17221 gcgtatttat gaagaatacg cgtcgttatt taccacacaa ctacgctcga tcctgcgcgc
    17281 ctccgctcac ggcagcctga aaatcatgat cccgatgatc tcctcaatgg aagagatctt
    17341 atgggtgaaa gaaaaactgg cagaagccaa acagcaacta cgtaacgaac acattccgtt
    17401 tgatgagaag atccagctcg gcattatgct ggaagtgccg tcggtgatgt tcatcatcga
    17461 tcaatgctgc gaagagattg atttctttag tattggtagt aatgacctga cgcaatatct
    17521 gctggcagtg gatcgcgata acgctaaggt tactcgtcac tacaacagcc tgaatccggc
    17581 attcttgcgg gcgctcgatt acgacgtgca ggcggtgcat cgccagggta aatggattgg
    17641 tctgtgcggt gagctgggag cgaaaggttc cgtgctgccg ttgctggtcg gcttagggct
    17701 ggatgaactc agcatgagcg caccatcaat tccggcggcg aaagcgcgga tggcgcaact
    17761 tgatagccgt gagtgccgca agttgctcaa ccaggcaatg gcctgccgta cttcgctgga
    17821 agtggaacac ctgctggcgc aattccgcat gacccaacag gacgcaccgc tggtcaccgc
    17881 cgagtgcatc acgctggaaa gtgactggcg cagcaaagaa gaagtgctca aaggcatgac
    17941 cgataacctg ctgctggcgg gccgctgccg ctatccgcgt aaactggaag ccgacttatg
    18001 ggcgcgcgag gccgttttct ctaccggtct gggctttagt tttgccattc cacacagcaa
    18061 atcagaacac attgagcaat ccaccatcag cgtggcgcgt ctgcaagcgc cggtgcgctg
    18121 gggcgatgat gaagcgcaat tcatcattat gttaaccctg aacaaacacg ctgcgggcga
    18181 tcagcacatg cgcattttct cgcgcctcgc tcgccgcatc atgcacgaag aattccgtaa
    18241 cgcgctggtt aacgccgcct ctgccgacgc tatcgccagc ctgctgcaac atgaactgga
    18301 actgtaaaag gaaacatcat ggaactgtat ctggacaccg ctaacgtcgc agaagtcgaa
    18361 cgtctggcac gcatattccc gattgccggg gtgacaacta acccgagcat tatcgctgcc
    18421 agcaaggagt ccatctggga agtgctgccg cgtctgcaaa aagcgattgg tgatgagggc
    18481 attctgtttg ctcagaccat gagccgcgac gcgcagggga tggtggaaga agcgaagcgc
    18541 ctgcgcgacg ctattccggg tattgtggtg aaaatcccgg tgacttccga aggtctggca
    18601 gcaattaaaa tactgaaaaa atagggtatt actacacttg gcaccgctgt atatagcgcc
    18661 gcagaagggt tattagccgc actggcaggg gcaaaatacg ttgctccgta tgttaaccgc
    18721 gtagatgccc agggcgggga cggcattcgt acggttcagg agctgcaaac gctgttagaa
    18781 atgcacgcgc cagaaagcat ggtgctggca gccagcttta aaacgccgcg tcaggcgctg
    18841 gactgtttac tggcaggatg tgaatccatc accctgccct tagatgtagc gcaacaaatg
    18901 ctcaacaccc ctgcggtaga gtcagctata gagaagttcg aacacgactg gaatgccgca
    18961 tttggcacta ctcatctcta aaggagcaat tatggaccgc attattcaat caccgggtaa
    19021 atacatccag ggcgctgatg tgattaatcg tctgggcgaa tacctgaagc cgctggcaga
    19081 acgctggtta gtggtgggtg acaaatttgt tttagttttt gctcaatcca ctgtcgagaa
    19141 aagctttaaa gatgctggac tggtagtaga aattacgccg tttggcggtg aatgttcgca
    19201 aaatgagatc gaccgtctgc gtggcatcgc ggagactgcg cagtgtggcg caattctcgg
    19261 tatcggtggc ggaaaaaccc tcgatactgc caaagcactg gcacatttca tgggtgttcc
    19321 ggtagcgatc gcaccgacta tcgcctcgac cgatgcaccg tgcagcgcat tgtctgttat
    19381 ctacaccgat gagggtgagt ttgaccgcta tctgctgttg ccaaataacc cgaatatggt
    19441 cattgtcgac accaaaatcg tcgcgggcgc acctgcacgt ctgttagcag cgggtatcgg
    19501 cgatgcgctg gcaacctggt ttgaagcgcg tgcttgctct cgtagcggcg cgaccaccat
    19561 ggcgggcggc aagtgcaccc aggcggcgct ggcactggct gaactgtgct acaacaccct
    19621 gctggaagaa ggcgaaaaag cgatgcttgc cgccgaacag catgtagtaa ccccggcgct
    19681 ggagcgcgtg attgaagcga acacctattt gagcggcgtt ggttttgaaa gcggcggctg
    19741 gcggcggctc acgcggtgca taacggcctg accgctatcc cggacgcgca tcactattat
    19801 cacggtgaaa agtggcattt ggtacgctga cgcagctggt actggaaaac gcgccggtgg
    19861 aggaaatcga aaccgtagct gcacttagcc atgcggtagg tttgccaata actctcgctc
    19921 aactggatat taaagaagat gtcccggcga aaatgcgcat tgttgcaaaa gcatcttgtg
    19981 cagaaggtga aaccatccac aacatgcctg gcggcgcgac gccagatcag gtttacgccg
    20041 cactgctggt agctgaccag tacggtcagc gtttcctgca agagtgggaa taacctactc
    20101 caaactcccg gcttgtccgg gagtttgaac gcaaaattgc ctgatgcgct acgcttatca
    20161 ggcctacgca atctctgcaa tatattgaat ttgcgtgctt ttgtaggccg gataaggcgt
    20221 tcactccgca tccggcactc tgtgccagta atctgaaact cccacgttgt tcaggagttt
    20281 tttatccgct tctggacatt ttctccacag aacaatcgtt agcccggaag gtcgaatctg
    20341 tgactcttat tttcacggac gaaccagatg aaagcttcac tggcgctgct cagcctgcta
    20401 acggcattta ccagccactc cttaaaatct cccgccgttc caccaaccgt ggtacaaatc
    20461 caggccaata ccaaccttgc catcgcagac ggtgccagac agcaaattgg cagcacgcta
    20521 ttttacgatc gcagcttacc tatcctggcg gtgatgttcc gcaagaacgc ggtgtatgtt
    20581 ccgatatggt gatccgcgca ttgcgcagcc agaaggtcga tttgcagaaa ctggttcacg
    20641 aagatatggc gaagaatttt gctgagtacc cgcaaaaatg gaagttaaag cgcccggaca
    20701 gcaacatcga tcaccgtcgg gtgcctaatc tggaaacctg gtttagccgc cacaacaaaa
    20761 cacgccccat cagtaaaaat gccggtgact atcaagcggg cgatattgtc tcctggcgat
    20821 tagataacgg gctggcgcat attggggtgg cgtcagatgg cttcgcccgc gacggtacgc
    20881 cgctggtgat acataacatt ggcgcaggcg cgcaggaaga agatgtgctg tttagctggc
    20941 ggatggtggg gcattatcgt tattttgtta aataagagat cgccccacat gaagaaaaaa
    21001 ttcagatgct ttttacactg acagcttctt ttttcttacg gctcaccagc gccagtgcca
    21061 gcacaatcag cacaataccg ctaccttcaa cggcccccgg attctcaccg agcagccacc
    21121 aggaaaagag tacgccacaa acgggaacgg ccagtgtgct taagctggca atactggcag
    21181 gcaagttttt cagcacaaac agccataagc tccacgctag cgccgtcgcc agaatcgcac
    21241 tgtaggccag cgcccagaac acggtgggct gccagtcaat ttcacgttgc ggcaccagta
    21301 aagcgaccac actcatcacc agcgccgcat acagcatctg ccaggatgtt aacgataata
    21361 aatccacgcg cggatgacgg gcgtataggc gtttagcaac aatcgcgctc gccccccaac
    21421 taacaccgga gaggattgcc agcatggcac ttttcatcga agagaaatcg agttgccacg
    21481 gctgcaacac caaaaataag ccgaaagcgg caatcagaat cgcgaaatat tgcccacgtc
    21541 gcaggcgttc accgagaaac aacgcggcga aaatcaccac ccagaacggc atggtatagc
    21601 tcaggatcgc caccttcccc gcacctccgc tgaccaacgc ccactgcgcc agaccaacca
    21661 tcccgcaggt ttgtaacagg gcaatggcta aggtgtattt aaacggcgtc gggcgcattc
    21721 cgcgaccacg taataaaagg acgatgaata aaacgagagc gccgaaaatg cagcgtaagg
    21781 cggtaaagtc gaaggcaccg atgtaactgg tgacttgctt catgaaaatc cagctataac
    21841 tccagataag tgtgagcaca accaagccac tgattgccag tgggttgctc tttcctgcgg
    21901 cagacatgag agatccagtg tgtagttttc ctgtggacac tatacgccgg taaagaaact
    21961 tcagcgactg cctgaaagac agtcgctgaa gaaggtcaga ttacagcagg tcgaaacggt
    22021 cgaggttcat cactttcacc catgccgcta cgaagtcttt aacaaacttc tcgtgggcat
    22081 cgctgctggc gtaaacttcc gctactgcac gcaggacaga gttagaacca aataccagat
    22141 ccgcacggct ggctgtgtat ttcacttcgc cggtttcgcg gtcacgtcct tcgaacagct
    22201 ctttcgattc gtcagtcgct ttccactcgt aacgcatatc cagcaagttc acgaagaagt
    22261 cattgctcaa tacgccaacg cggtcagtga agacgccgtt tttgctgcca tcgaagttgg
    22321 cacccagtac acgcatcccg cccaccagcg cagtcatttc cggcgcggtc agcgtcagtt
    22381 gctgtgcttt gtcgatcagc agtgactcgg tggtggaaac gtccagacga gcgcgatagt
    22441 tacggaaacc gtcagcaatt ggctccagca gctcaaacat ctcaatgtca gtctgatcct
    22501 gacgcgcatc aacgcgaccc ggcgcaaacg gtacatgaat gctcaaacct gcggcgcttg
    22561 cggctttctc aacaccaacc acaccagcca gcacgatgat atccgccagc gaggctttac
    22621 cagactcttt ctggattttc tccagaacag gcagagcacg aacggctgcg gcgttcacat
    22681 cccagtcgcg ctgcggcatt aatgccagac gcgcaccgtt ggcaccaccg cgtttgtcgc
    22741 caccacggaa ggtagaagca gatgcccagg ccaccgatac cagctcacta acagacagac
    22801 cagaatccgc aatcgcgaat ttcagatcga taatgtcctg ctcggtcggg ttgtagatcg
    22861 gctgcggcag cggatcttgc cagatcagat cttctttcgg cacttccggc ccgatgtagc
    22921 gagatttcgg ccccatatcc ctgtgcgtca gtttgaacca ggcacgggca aaggcttcgt
    22981 tgaacgcctg cggatcgttg aggaaacgac gagagatctt ctcgaactca ggatcaaaac
    23041 gcagcgtcag gtcggtcacc agcattgtcg gtttacgttt cttcgacgga tcgaacggat
    23101 ccgggataat ttccggtgcg tctaccgctt cgaactggat tgctccagcc ggactgcggg
    23161 tctgtaccca ctcatacttg aacagattct cgaagaaata gttgctccac tggatcggcg
    23221 tctgggtcca gactacttcc agaccagagg taatggcatc tgcgccaacg ccgctgccgt
    23281 aagtgctcgc ccagcctaaa ccttgttctt caatcggtgc agcttctgga tcaggaccta
    23341 cgtttgatgt cggaccggca ccgtgggttt tacccagcgt atgaccaccc gcaatcagcg
    23401 ccacggtttc ttcgtcgttc atgcccatgt tgccgaaggt cgcgcggata gctgctgccg
    23461 cagaaagcgg ttcgccgctg tgatccgggc cttccgggtt aacgtaaatc aggcccatct
    23521 cggttgcacc cagcggtgct ttcgccagcg cttccggatg acggtgagtc agccaggctt
    23581 tttcatcacc ccagttaaca tccagatccg gttcccagac gtcttcacga ccggcaccaa
    23641 aaccgaaggt acggaagccg gagttttcca gcgccacgtt acctgcgagg ataaacaggt
    23701 cggcccagga gattttctga ccatatttct gtttgattgg ccacaacagg cgacgcgctt
    23761 tatcgaggct tacgttatcc ggccaggagt tcagcggtgc aaaacgttgc tgaccacgac
    23821 ccgcgccgcc gcgtccatcg attgaacggt aagtacctgc gccgtgccaa gccatacgaa
    23881 taaacagacc ggcgtagctg ccccagtcgg ctggccacca cggctgagat tctgtcagca
    23941 gggctttcag atcttttttc aggccgtagt aatctaattt gctgaattct ttgcggtagt
    24001 caaagtcctc acccagcggg ttagaacgat tagaatgttg gtttaacagg tcaacacgga
    24061 gttgatttgg ccaccagtcg cgagtggttg tgcccgcccc cgcgctctgg tcgtgaccgc
    24121 cctgatggaa cgggcatttg ccagtggctg tggtgttatg gatatcgtct gacgtgctca
    24181 tcaatgtgct cccctctaca gtgttaccgt tacgatacac agcgttagag agaagttata
    24241 attgaattaa tccacggatg cgatagttga gatcttacat attgttggtt aaagagatgt
    24301 agatcaaatt gatcgtaatt aagctttttt gattatgtcc gaattcggac aaatgcttta
    24361 taaaaaaggg ttttagcttc atatccttca agtaatgagg gagaaaacaa ggctgacaac
    24421 cttattttcc cgccctcatt tcgaggcagc attttgtgct ctgtttaaaa tttgtgatca
    24481 ctgtgtgatt ttcacaaaag ccacactatt tataaaccag gtcgaacacc cagcgtatgg
    24541 caaatcgcgt aactcatttc agcgcggtta agcgtataga agtggaaatc tttcactcct
    24601 tcacggctta aaatcttcac catatccatg gcaatattcg cgcccaccag tttgcgggtt
    24661 tcggcatcgt catccagccc gtcaaacatt tgcgccatcc acgccggaat acgcacgttg
    24721 gtcatgtcgg caaatttctt cgcctgttta aagttagata ccggcaaaat gcccgggatg
    24781 atttccacat caatgcccgc cgatacacag cggtcacgaa aacgcaggta gctttcgaca
    24841 tcgaagaaga actgagtaat cgcgcggttg gctccggcat ccactttgcg tttcagatta
    24901 agcaaatccg cctgagcgct ttttgcttcc gggtgaactt ccggatacgc cgccacggat
    24961 atatcgaaat ctgccacttc ttttaacagc gtcaccaggt cagaagcata catttctggc
    25021 ttaccacttc ccggcggcag atcgccacgc agtgccacga tatgacgaat accgttattc
    25081 cagtagtcgc gtgcaatggt gcgcagctcg tcgggcgtcg catcaatgca agtaagatgc
    25141 ggtgccgctt ccagaccagt gcgatcttta atgcctttaa taatgctgtg cgtacggtcg
    25201 cgctcgccgg agttcgcgcc ataggtcacc gatacaaact tcggtttcag gctgctaagg
    25261 cgatcgatgg agttccacag ggtctgctcc atttcactgg tacgcggcgg gaaaaactcg
    25321 aacgaaacgt taatctgccc ctggacttct gccaggctct gattcagggc atcccgctgg
    25381 ctggcgtgaa aaaagctcat accttacctc atcaatcgct tgtagttata tgttgtgttg
    25441 tgcacatcta tccatttaga cgtccagatg taaagatgaa ggaaaagccg aagggcgtca
    25501 accgaaaaat gaccaatgat ggtgaggaaa aatcagggaa gatgaaaaaa cttcaggatg
    25561 gtaagaaaaa gaaaatccga tatgcaaagc aaaccggact gcaaatataa actaaccatg
    25621 tacttattgc ttcttgccgg attcgtcgta aacgcttcat ccggcctaat tctatgataa
    25681 ttcaatatat ttcattagaa accatagacc cgaaaacatg agtaccgggc attattaaat
    25741 ttctgaaatt acaacaactg tgccagccgg ttgatatccg actgaatcgc cccggcggtg
    25801 acgtcgcgcc cagcgccagg cccacggatc accagcggat tatcgcggta ccagcggctt
    25861 tcgatggcaa agacgttatc gcatggcagc agtgatgcca gcggatagtc ttcacgcacc
    25921 gcctccacgc ccacacgcgc tttgccgttg gcatcgaaac gcgccacgta gcgcagcacc
    25981 agccccattt cgcgggccgc ttccagccgt tgcaccatct gctcgttaag ttcatcgcca
    26041 ttttcaaaga aatggtcgat gctgccgcct tcgcaatgag caggcaccag cgattccaca
    26101 cgtacctggt ccggttcaat gttgtaaccc gcttcacgcg ccagaatcac cagcttgcgc
    26161 attacgtctt tgccggagag gtcgtcacgc ggatcaggct cggttaagcc ctgctgccac
    26221 gcctgatcca ccagctcggt aaacggcacg ctaccgtcga attgcaggaa cagccaggag
    26281 agcgtgccgg agaagatccc actgatcgac aaaatggtat cgccgctgtc gatcagatcg
    26341 cgcacggtat ggttgatcgg caagcccgca ccaacggtgg cgttgtatag ccagtgacgc
    26401 ccggtttttt cgaaggcgtc gtggatctgg cgatatttat tgctgtcgct tgctcccgcc
    26461 agtttgttgg cactgatgac gtggaaaccg tggctggcga aatcgagata ctgatcagca
    26521 agctgctggc tggcggtaac gtccaacacc actaaatcat catacggatg ggcgcgcatc
    26581 cacaggaaaa gcgactcttc atcctgctcg accgcttcat cgttgaagaa ggctaacgcg
    26641 cggctggcgt ccagcccgtc ataactcaac agactgcggc ggctgtccac cacgcctgcc
    26701 agcacaaatt caaagccggt acgcgccgaa agcgtgctct gctcgcgggc gaacagttcc
    26761 agccagcgag agccgatatt gcctttgccg aacaatacca ggccgatgcg tttttctgcg
    26821 cggaagacgg actgatgcag cccctgaatc aggctttcgg tcgggccggt gcgcagtact
    26881 gccaccagac tgatgccgtc atcggactgc cacgtaaact cgaccggctg gcctttcagt
    26941 tgctgccaga agcggtggca atgcagcggg ttacgggtga cacccgcccc gaccatcgcc
    27001 accagcgcca gcccctgacg caggcgcagt tctccaggta atcccgcttc gtcgaggatt
    27061 ttcagcgcac tgtcggccac ttctgaggtg tagcaaaatt gcaacaactg gcgatcgtta
    27121 tgcacgccaa ccgccagtgg acgcacctgg gcacgtttca ggatctggtc gatctcttta
    27181 tgcgccagtt tgaaatcctg actggcgggc acctgaaact caatcaaaca gacatcatcg
    27241 tggctggtga caatacgcgc accagtaccg gaggccagca cgcgttcaat gcgcgtggaa
    27301 ccttgatccg gcgtgtagct acagcgcagc tgcaggtcga tttcgctgcc agaaactggc
    27361 tgtaaagtac gggcgtgaag aacgggagcc gccagacgcg ccagttcgct ggcctcatcc
    27421 agacgcagca acggcagcag gcaggcatct ttcactttac gcgggtcggc actgtatacc
    27481 ccggcgacgt cgctccagat ggttacacga gaaacacccg ccagcgcacc gatttgtgtc
    27541 gcggaatagt cggaaccgtt acgccccagc aacaccgttt caccggcgtt gttacggctg
    27601 ataaacccgg tcaccaccag acgtttgctc ggatgttgta ccagtagctg ttgcagcaac
    27661 gggtaagaaa gcccttcatc aacctgcggt tgcgcagcgc gttcagcgcg taaaaactca
    27721 cgggcatcaa gccaggccgc tggcagccct tgttgattaa gtaccgcaga catcaggcgc
    27781 gccgaccaca cttcaccgtg gcccaccact tccgcataca ccgcatcgtt aataccactg
    27841 tcgagcaacg ccgccaggcg ctcaagatcg ctgacaaaag cgctaatgag gctatcggct
    27901 tcttcagcgg gtagcagacc gctaatcaga tcgcactgat agcgacgcag cgtttgttga
    27961 acctgatgcg cagagagacg atcggtctgg cttagtttca accagttaat caactggtta
    28021 gtggtgctac cggcggcgga aaccaccatc atatcgtcag gctgagagaa ctccgccata
    28081 atgcccgcga cacgcaaata acacttcaca tcagccagac tactgccacc aaatttatgc
    28141 agctgacgac ctttcgcccc tgcctgcgca atcacactca tttttacccc ttgtttgcag
    28201 cccggaagcc attttccagg tcggcaatta aatcttcgcc atcttcaata ccggtggaga
    28261 tacgcagcag cgtctcggag atcccggcgg cagcacgcgc ttctggtgcc atgcctgcat
    28321 gtgtcatggt tgcggcgtga gagattaaac tttccactcc ccctaatgat tccgccagcg
    28381 taaacaacga cagcccgccc aggaaacgac acagcgtctg ctcatcgcca tccagttcaa
    28441 aactcaacat tgcgccaaag cctttttgct gacgcgcggc aatttcatgc ccctgatttt
    28501 ccggcagcga cgggtgatac agttttttca ccaacggctg ggtttgcaga tatttcacaa
    28561 tcgcctgcgc gttgcgctgc gccagctcca tacgcggcac cagcgttcgc aacccgcgta
    28621 gcagcagata gctgtcaaac gcgccgcccg tcacgccaat attgtttgcc caccaggcaa
    28681 gttcagtgac aacgtccggg tctttagcaa tcaccacgcc agccaccacg tcagagtgac
    28741 cgttcagata tttcgtacat gaatgcaaca ccagatcggc acccaatgcc agcggatttt
    28801 gtaatgccgg gcttaagaag gtgttatcca ccacgctcac cgccccgact tcccttgcca
    28861 gatggcagat tttcgcaata tccacgacgc gtaacaatgg attacttggg ctttctacca
    28921 gtaccagttt gggtttttct gccagcgctg cccgtaatgc ctgttcatcg ccttgatcaa
    28981 caaacaacac gcgatagcaa ccgcgtttcg ccagactgtc gaacaggcga tagctaccgc
    29041 cgtagcagtc gtgcggcgca accagcagat cgccaggttt caaaaagacg gtcgttacca
    29101 ggtgaatcgc ggacatgccg gtattagtaa gtactgcgcc agcaccgcct tccagttctg
    29161 ccagcgcacg ctgaaccaca tcgcgcgttg ggttgccgcg acgcgagtaa tcatgcgcgc
    29221 gcggttcatt aaatccggta aagttatagg tgctggaaag atggatggga gggacaacgc
    29281 aaccatactg ttcgtcgtca tttaacccgc tacgcactgc gatggtggcc tgtttacgcg
    29341 tcatgtgatg aagtttcctg ggctttgtcg gtgaaatgtc aggcaccaga gtaaacattg
    29401 tgttaatgga cgtcaataca tctggacatc taaacttctt tgcgtataga ttgagcaaat
    29461 cccaaatagc cgttaaaatt atatgcatta tcacgccgac aggtgcatta cacgatgtca
    29521 cggtaacgcc tgtacggtaa actatgcggg tttacggtca gtacccacat aaattgtggg
    29581 gtctggtctc aatttattga cgaagaggat taagtatctc atggctgaat ggagcggcga
    29641 atatatcagc ccatacgctg agcacggcaa gaagagtgaa caagtcaaaa agattacggt
    29701 ttccattcct cttaaggtgt taaaaatcct caccgatgaa cgcacgcgtc gtcaggtgaa
    29761 caacctgcgt cacgctacca acagcgagct gctgtgcgaa gcgtttctgc atgcctttac
    29821 cgggcaacct ttgccggatg atgccgatct gcgtaaagag cgcagcgacg aaatcccgga
    29881 agcggcaaaa gagatcatgc gtgagatggg gattaacccg gagacgtggg aatactaaag
    29941 cggaaaagag cggcgcggag tggaatcgcc tgatgcacta cgcttatcag gcctacgtca
    30001 tattgcaatt tattgaattt gcacgaactt gtaggccgga taaggcgttc acgccgcatc
    30061 cggcataaac aacgagcacg ttgtctgcga cccaccgctt tttatacatg gacgtttaac
    30121 tatgaaaaac aggctgctga tcctcagcct gctggtttct gtacctgcct ttgcctggca
    30181 gccgcaaact ggcgacatca tctttcaaat ctctcgctca tcgcaaagta aagcgatcca
    30241 actggcaacc cattccgatt acagccacac cggtatgctg gtgatgcgta acaaaaagcc
    30301 ctacgttttt gaagcggtcg gcccggtgaa atacaccccg ctcaagcagt ggattgccca
    30361 tggtgaaaag ggcaactacg ttgttcgccg cgttgaaggc ggactgagtg ttgaacaaca
    30421 gcaaaaactg acgcaaacgg caaaacgtta tctcggtaaa ccgtatgatt tcagcttctc
    30481 atggagcgac gatcgccagt actgttcgga agtggtgtgg aaggtttacc agaacgcgct
    30541 gggaatgcgc gtgggcgaac agcagaagtt gaaagagttt gatctcagca acccgctggt
    30601 ccaggcgaag ctcaaagaac gctacggtaa aaatattccg cttgaggaaa cggtagtttc
    30661 accacaagcc gttttcgacg cgccacaact caccacggtt gccaaagagt ggccgctgtt
    30721 ttcgtggtga tacgaccaaa attcaggcac aaaaaaagcg ccgtgcggcg cttttttcgg
    30781 aaatccggtc ttatttgctg cccgggatgt tgaaacgctt gttgaagcgg tcaacacggc
    30841 caccggtagc aacatcacgc tgtttgccag tgaagaacgg gtggcacttg ctgcacacgt
    30901 cgaggttcag gtcatgacca acggtggagc ggatttttat tacgttaccg caagagcagc
    30961 tagcagtaat ttcttcgtat ttcgggtgaa tatctttttt catgggaaaa cctcggttta
    31021 aggccgcgtc gctcttccag ccctaacgcc agacaccacg cgatgttaaa agtatagctt
    31081 caatacgatc atttcgtacg aaggcgcgaa atcatacaga aattaaccag cgtatgcaaa
    31141 ctgatccgca ctcttctacg gcaatgtgta tactaaccca ccgaatttca agtcaggatg
    31201 atgctatgcc cgttgcccac gttgccttgc ccgttccgct tcctcgtacc tttgactatc
    31261 tgctgccaga aggcatgacg gttaaagctg ggtgtcgcgt gcgcgtgccg tttggcaaac
    31321 agcaggagcg catcgggatt gtggtatcag ttagcgatgc cagcgaactg ccgctcactg
    31381 agctaaaagc ggtagtcgaa gtgctggatg gtgagccggt gtttactcac tccgtctggc
    31441 gattgctgct atgggcggca gattactatc atcatccgat tggcgatgtg ctgtttcatg
    31501 ccttgccgat tttactacgc caggggcggc ctgcggcgaa cgcgccgatg tggtactggt
    31561 ttgccactga acaaggtcag gcggtggatc tgaacagcct gaaacgctca cccaagcaac
    31621 aacaggcgct ggcagcgtta cggcaaggca aaatctggcg cgaccaggtt gccacgctcg
    31681 aatttaatga tgccgcgttg caggcgctgc gcaaaaaagg tctgtgtgat ttagcaagtg
    31741 aaacaccaga gtttagcgac tggcgaacga actatgccgt ttctggtgag cggttgcgat
    31801 tgaataccga acaggccacc gccgttggcg caattcatag cgcggcagat actttttctg
    31861 cctggctgct ggcgggcgtt accggttccg gtaaaacgga ggtttatctc agtgtactgg
    31921 aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt gccggaaatc ggcctgacac
    31981 cgcaaactat cgcccgtttt cgtgaacggt ttaatgcgcc cgtggaagtt ctgcattccg
    32041 gtctgaacga cagcgagcgt ctttcggcgt ggctgaaagc gaaaaatggc gaggcggcga
    32101 ttgtgatcgg cacccgctcc gcgctgttta cgccgtttaa aaatctcggc gtgattgtca
    32161 ttgacgaaga gcacgacagt tcctacaagc agcaggaagg ctggcgctat catgcccgcg
    32221 acctggcggt gtatcgtgcg catagcgagc aaatcccgat tattcttggc tccgcaacgc
    32281 ccgcgctgga aacgttgtgc aacgtccagc agaaaaaata ccgcctgctg cgcctgacgc
    32341 gccgggcggg gaatgcgcgt ccggcaattc aacacgtgct ggatttaaaa ggtcagaagg
    32401 tgcaggcagg tctggctccg gcgttaatca cccgtatgcg ccagcattta caggccaaca
    32461 atcaggttat cctctttctt aaccgccgtg gctttgcgcc tgcgctactg tgccacgact
    32521 gcggctggat tgccgaatgc ccacgttgcg atcactacta cacgctgcat caggcgcaac
    32581 agcatttgcg ctgtcaccac tgtgacagtc agcgtccggt gccgcgccag tgcccttcct
    32641 gcggttccac gcacctggta cccgtggggc tgggcaccga acagcttgaa cagacgcttg
    32701 cgccgctgtt ccctgatgtg cccatttctc gtatcgaccg cgataccacc agccgcaaag
    32761 gggcgctgga acagcaactg gcggaagtac atcgcggcgg tgcgaggatt ttgatcggca
    32821 cacaaatgct ggcgaaaggt caccactttc cggatgtgac gctggtggcg ttactggatg
    32881 tggacggcgc gctgttttct gccgattttc gctcggcaga gcgtttcgct cagctttaca
    32941 cccaggtcgc tggtcgtgcc gggcgtgcgg gtaaacaagg cgaagtggtg ctgcaaacgc
    33001 accatccgga acatcctctg ttgcaaacgt tgctctataa aggctacgat gcctttgccg
    33061 aacaggcgct ggctgagcga cggatgatgc aactgccgcc atggaccagc catgtgattg
    33121 tgcgtgcgga agatcataac aatcagcatg cgccgctgtt cctgcaacaa ctgcgtaatc
    33181 tgatcctctc cagcccgctg gcagacgaca aactgtgggt tctcggtccg gttccggcac
    33241 tggcacctaa acgtggcggt cgctggcgct ggcagatatt gttgcagcac ccttcccgcg
    33301 tgcgcttgca acacatcatt agtggtacgc tggcgctcat caatacaata ccggattccc
    33361 gcaaggtgaa atgggtactg gatgttgatc cgattgaggg ttaaaccgct tacgatgcga
    33421 ggcggatcga aaaattcaat attcatcaca cttttcatga aaattctgta accgttttca
    33481 cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca tggatgtagt acgcctgacg
    33541 tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa actgccgcga ccatgaaaga
    33601 cgttgccctc aaggcaaaag tctctacagc gactgtctcc cgagcattaa tgaatcccga
    33661 taaagtctcc caggccaccc gtaatcgggt tgaaaaagcg gcccgggaag tgggttattt
    33721 accgcagcct atggggcgca acgtcaagcg taatgaatcc cgcaccattc tggtgattgt
    33781 cccggatatc tgcgatccct tctttagcga aattattcgc ggtatcgaag ttacggcggc
    33841 aaatcacgga tatctggtgc tgattggcga ctgtgcgcat caaaatcagc aggaaaaaac
    33901 ctttatcgat ttgatcatca ccaagcaaat tgatggcatg ttgctgctgg gttcaaggct
    33961 gccgtttgat gccagcattg aggaacagcg taatctgccg ccgatggtga tggcgaacga
    34021 atttgcaccg gaactggagc tgcctaccgt tcatatcgac aatctgaccg ccgcatttga
    34081 tgcagtaaat tatttatatg agcaagggca taaacggatt ggctgtatag ccggtcccga
    34141 agagatgcca ctgtgtcact accgcctgca aggctatgtt caggcgctgc gtcgctgcgg
    34201 aattatggtt gatccacaat acatcgcccg tggcgacttc accttcgaag ccggaagcaa
    34261 agcaatgcaa cagctgcttg atcttccaca accgcctact gcagtcttct gccatagcga
    34321 tgtgatggcg ctcggcgcac tttctcaggc aaaacgccag gggctgaaag tcccggaaga
    34381 cctttccata atcggttttg ataacatcga cctgacgcaa ttttgtgatc cgccgctgac
    34441 aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct atgctgttat tgcttgatca
    34501 aatgcagggg caacacgttg gcagtggctc tcgtttaatg gactgcgaac ttatcatccg
    34561 gggatcaaca cgcgcgttac cttaaagtaa acctttaaga ctgccgtgac tggtcaaagg
    34621 cccgccgctt aagtaacatg gcgggctgac gaacgaataa atacagcgaa acgatagtgg
    34681 cacaacgaga ttatgtacgc cgcagccaac cggcaccttc gcggcgaaaa aagagcacct
    34741 cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc tatggtcgct attgctgccg
    34801 ccgttcttgt gacctttatc ggtggtctgt acttcattac gcatcacaag aaagaagagt
    34861 ccgagacgct gcaaagccag aaagtgaccg gaaacggact accaccaaaa ccagaagaac
    34921 gctggcgcta cattaaagag ctggaaagtc gccagccggg tgtgcgtgcg cccacagaac
    34981 cttctgccgg tggtgaagtg aaaacgccgg agcaactgac accagaacaa cgtcagcttc
    35041 ttgaacaaat gcaggctgat atgcgccagc agccaacgca gctggttgag gttccgtgga
    35101 atgaacagac gccggaacag cgtcagcaaa cgctacaacg ccagcgtcag gcgcagcaat
    35161 tagcggagca gcaacgcctg gctcagcagt cgcgtacgac tgaacaaagc tggcagcagc
    35221 agacgcgtac gtcgcaagcc gcgccggtac aggcgcaacc gcgccagtct aaaccagctt
    35281 ctactcagca gccgtaccag gatctgctgc aaacccctgc gcacacgacc gcgcagtcga
    35341 agccgcagca agctgcgccg gttgctcgtg ccgctgacgc gccaaaaccg acggcggaga
    35401 aaaaagacga acgccgctgg atggtgcagt gcggttcgtt cagaggcgcg gaacaggcag
    35461 agacggtacg tgctcagctg gcgttcgaag gatttgactc gaaaatcacc accaacaatg
    35521 gctggaatcg tgtggtcatt ggcccggtga aaggcaaaga gaacgcagac agcaccctca
    35581 atcggttgaa gatggcgggt catacaaact gcattcggct cgccgccggg ggttgaaacc
    35641 ctcaaaatcc cccccatcta taattgcatt atgccccgta cttttgtacg gggtttgtac
    35701 tctgtattcg taaccaaggg gtcagctcgt gacaactata gtaagcgtac gccgtaacgg
    35761 ccatgtggtc atcgctggtg atggtcaggc cacgttgggc aataccgtaa tgaaaggcaa
    35821 cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc gcgggctttg cgggcggtac
    35881 tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa ctggaaatgc atcagggcca
    35941 tctggttaaa gccgccgttg agctggcaaa agactggcgt accgatcgca tgctgcgcaa
    36001 acttgaagca ctgctggcag tcgcggatga aactgcatcg cttatcatca ccggtaacgg
    36061 tgacgtggtg cagccagaaa acgatcttat tgctatcggc tccggcggcc cttacgccca
    36121 ggctgcggcg cgcgcgctgt tagaaaacac tgaacttagc gcccgtgaaa ttgctgaaaa
    36181 ggcgttggat attgcaggcg acatttgcat ctataccaac catttccaca ccatcgaaga
    36241 attaagctac aaagcgtaag gatctcccat gtctgaaatg accccacgcg aaatcgtcag
    36301 cgaactggat aagcacatca tcggccagga caacgccaag cgttctgtgg cgattgctct
    36361 gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg cgccatgaag tgaccccgaa
    36421 aaatatcctg atgatcggcc cgaccggtgt cggtaaaact gaaatcgccc gtcgtctggc
    36481 taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc aaatttaccg aagtgggcta
    36541 cgtcggtaag gaagttgatt ctattattcg cgatctgacc gatgccgccg tgaaaatggt
    36601 acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa gaactggcag aagaacgtat
    36661 tctcgacgtg ctgatcccac ctgctaaaaa caactgggga cagactgaac agcagcagga
    36721 accttccgct gctcgtcagg cattccgcaa aaaactgcgt gaaggccagc ttgatgacaa
    36781 agaaatcgag atcgatcttg ccgcagcacc gatgggcgtt gaaattatgg ctcctccggg
    36841 catggaagag atgaccagcc agctgcagtc catgttccag aacctgggcg gccagaagca
    36901 aaaagcgcgt aagctgaaaa tcaaagacgc catgaagctg ctgattgaag aagaagcggc
    36961 gaaactggtg aacccggaag agctgaagca agacgctatc gacgctgttg agcagcacgg
    37021 gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc ggcgagtctt ccggtccgga
    37081 tgtttctcgt gaaggcgttc agcgtgacct gctgccgctg gtagaaggtt gcaccgtttc
    37141 caccaaacac gggatggtca aaactgacca cattctgttt atcgcttctg gcgcgttcca
    37201 gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt cggctgccaa tccgcgttga
    37261 actgcaggca ctgaccacca gcgacttcga gcgtattctg accgagccga atgcctctat
    37321 caccgtgcag tacaaagcac tgatggcgac tgaaggcgta aatatcgagt ttaccgactc
    37381 cggtattaaa cgcatcgcgg aagcggcatg gcaggtgaac gaatctaccg aaaacatcgg
    37441 tgctcgtcgt ttacacactg ttctggagcg tttaatggaa gagatttcct acgacgccag
    37501 cgatttaagc ggtcaaaata tcactattga cgcagattat gtgagcaaac atctggatgc
    37561 gttggtggca gatgaagatc tgagccgttt tatcctataa tcgcgttcaa tcattttcat
    37621 cattgtttga tggggctgaa aggccccatt tttattggcg cgtattatga ctgaacaaca
    37681 aattagccga actcaggcgt ggctggaaag tttacgacct aaaaccctcc ccctcgcctt
    37741 tgctgcaatt atcgtcggga cggcgctggc atggtggcaa ggtcacttcg atccgctggt
    37801 cgccctgctg gcactaatta ccgccgggct attacagatc ctttctaacc tcgccaatga
    37861 ttacggcgat gcggtaaaag gcagcgataa acctgaccgc attgggccgc tacgcggcat
    37921 gcaaaaaggg gtcattaccc agcaaaagat gaaacgggcg ctcattatta ccgtcgtgct
    37981 catctgtctc tccgggctgg cactggttgc agtggcatgc catacgctgg ccgattttgt
    38041 cggtttcctg attcttggcg ggttgtcgat cattgccgct atcacctaca ccgtgggcaa
    38101 tcgtccttat ggttatatcg gtctgggtga tatttccgta ctggttttct ttggctggtt
    38161 gagtgtcatg gggagctggt atttacaggc tcatacattg attccggcac tgatccttcc
    38221 ggcgaccgca tgcggcctgc tggcaacggc agtactgaat attaataacc tgcgtgatat
    38281 caatagcgac cgcgaaaatg gcaaaaacac gctggtggtg cgcttaggtg aagtgaacgc
    38341 gcgtcgttat catgcctgcc tgctgatggg ctcgctggtg tgtctggcgc tgtttaatct
    38401 cttttcgctg catagcctgt ggggctggct gttcctgctg gcggcaccat tactggtgaa
    38461 gcaagcccgt tatgtgatgc gggaaatgga cccggtggcg atgcgaccaa tgctggaacg
    38521 tactgtcaag ggagcgttac tgactaacct gctgtttgtt ttagggatat tcctaagcca
    38581 gtgggcagca taactgacaa atatcaatta acaattgatg attttgccaa cagcccacat
    38641 agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta agcctatgaa atacgatact
    38701 tccgagcttt gtgacatcta tcaagaagat gttaacgtcg tggaaccgct gttctccaac
    38761 tttggcggac gggcgtcgtt tggcggacaa ataatcacgg taaaatgttt cgaggacaac
    38821 gggttgctgt acgatctgct cgaacagaat ggccgtggtc gtgttcttgt cgtcgatggc
    38881 ggtggttctg ttcgtcgcgc actggtcgat gctgaactgg cgcgtctggc agtacaaaat
    38941 gaatgggaag gtctggtcat ttacggcgcg gtgcgtcagg tagatgacct ggaagagttg
    39001 gatatcggca tccaggcgat ggcggcaatt ccggttggtg ccgctggcga aggcattggc
    39061 gaaagcgatg tccgcgtcaa ttttggcggt gtcaccttct tctccggcga ccatctttat
    39121 gccgacaata ccgggattat tctttcagaa gatccgctgg atattgaatg ataagaaagg
    39181 caccgcaagg tgcctttttt ctgcgttacc tgttggccta cacagtaaag aaattacgcg
    39241 aaagatgaag cgtaatcaga cctcttccat gcgacccagc agggcctgca gacgttcctg
    39301 ccagccgttc tgctgttctt tcagatggtt gttctcacgc tccagctctt cgcgctgatg
    39361 ctgggcattt tgaacttcct gcgacagtga gttgtttttt tctttcagct cttcgatttc
    39421 catctgcaac agagtgatgg tatcaatcgc ctgctgtact tttgcttcca gtttctcaaa
    39481 cacttctaat gacattgtca tacctctcct gaattgcaag gcgttgatgg ataaaaaatc
    39541 ctcgtcccga ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt
    39601 gtatgaagcc gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact
    39661 tttcatcctt ctcctagtga cataatccac atcaatcgaa aatgttaata aatttgttgc
    39721 gcgaatgatc taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg
    39781 ctcattttat gacgaagcac acacatttta agttcgatat ttctcgtttt tgctcgttaa
    39841 cgataagtta acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac
    39901 aaacattaac tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca
    39961 ttgctgaatt cctcggtacc gggttgttga tttttttcgg tgtgggttgc gttgcagcac
    40021 taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg
    40081 tggcaatggc catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta
    40141 ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt
    40201 cacaagttgc cggcgctttc tgcgctgcgg ctttagttta cgggctttac tacaatttat
    40261 ttttcgactt cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg
    40321 ctggcacttt ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg
    40381 agatggtgat taccgctatt ctgatggggc tgatcctggc gttaacggac gatggcaacg
    40441 gtgtaccacg cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg
    40501 catctatggg cccattgaca ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag
    40561 tctttgccta gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt
    40621 acttcctggt gccgcttttc agccctatcg ttggcgcgat tgtaggtgca tttgcctacc
    40681 gcaaactgat tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggaaacca
    40741 caactccttc agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact
    40801 gaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg
    40861 atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac
    40921 caggttgggt agaacacgac ccaatggaaa tctgggccac ccaaagctcc acgctggtag
    40981 aagtgctggc gaaagccgat atcagttccg atcaaattgc agctatcggt attacgaacc
    41041 agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg
    41101 tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag
    41161 attatatccg cagcaatacc ggtctggtga ttgacccgta cttttctggc accaaagtga
    41221 agtggatcct cgaccatgtg gaaggctctc acgagcgtgc acgtcgtggt gaattgctgt
    41281 ttggtacggt tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg
    41341 attacaccaa cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca
    41401 aaatgctgga agtgctggat attccgcgcg agatgctgcc agaagtgcgt cgttcttccg
    41461 aagtatacgg tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga
    41521 tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga
    41581 agaacaccta tggcactggc tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat
    41641 cagaaaacgg cctgctgacc accatcgcct gcggcccgac tggcgaagtg aactatgcgt
    41701 tggaaggtgc ggtgtttatg gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt
    41761 tgattaacga cgcctacgat tccgaatatt tcgccaccaa agtgcaaaac accaatggtg
    41821 tgtatgtggt tccggcattt accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg
    41881 gggcgatttt cggtctgact cgtggggtga acgctaacca cattatacgc gcgacgctgg
    41941 agtctattgc ttatcagacg cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc
    42001 gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa caatttcctg atgcagttcc
    42061 agtccgatat tctcggcacc cgcgttgagc gcccggaagt gcgcgaagtc accgcattgg
    42121 gtgcggccta tctcgcaggc ctggcggttg gcttctggca gaacctcgac gagctgcaag
    42181 agaaagcggt gattgagcgc gagttccgtc caggcatcga aaccactgag cgtaattacc
    42241 gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt
    42301 aatgtcaggc acctgaatcg attgcctgat gctgatcgcc tgatgcgacg cttacgcgtc
    42361 ttatcaggcc tacgagccaa tgccattttg tgtaggcggg ataaggcgtt cacgccgcat
    42421 ccggcatcac gcagactctg aaaccgtgcg ggggcaaccc cgcacacatc aataatccca
    42481 cccttcccct gtgctacact tcgcgccatt ccttactgct tagagtttgc tatgagacga
    42541 gaacttgcca tcgaattttc ccgcgtcacc gaatctgcgg cgctggctgg ctacaaatgg
    42601 ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat gcgtattatg
    42661 ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat cgacgaagca
    42721 ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt agatattgct
    42781 gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc gctggcggtg
    42841 ctggcagtag gcgataaagg ctgcttcctc aatgcgccgg atatgtatat ggagaagctg
    42901 attgtcgggc cgggagccaa aggcaccatt gatctgaacc tgccgctggc ggataacctg
    42961 cgcaatgtag cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac gattctggct
    43021 aaaccacgcc acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg cgtatttgct
    43081 attccggacg gcgacgttgc ggcctcaatt ctcacctgta tgccagacag cgaagttgac
    43141 gtgctgtacg gtattggtgg cgcgccggaa ggcgtggttt ctgcggcggt gatccgcgca
    43201 ttagatggcg acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg cgacaacgaa
    43261 gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg catcgaagcc
    43321 ggtaaagtat tgcgtctggg cgatatggcg cgcagcgata acgtcatctt ctctgccacc
    43381 ggtattacca aaggcgatct gctggaaggt attagccgca aaggcaatat cgcgactacc
    43441 gaaacgctgc tgatccgcgg caagtcacgc accattcgcc gcattcagtc catccactat
    43501 ctggatcgca aagacccgga aatgcaggtg cacattctct gagtgatttg atcgattgag
    43561 ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa ggcaagtcaa
    43621 tcaaaacagg agaaaaacat ggctgattgg gtaacaggca aagtcactaa agtgcagaac
    43681 tggaccgacg ccctgtttag tctcaccgtt cacgcccccg tgcttccgtt taccgccggg
    43741 caatttacca agcttggcct tgaaatcgac ggcgaacgcg tccagcgcgc ctactcctat
    43801 gttaactcgc ccgataatcc cgatctggag ttttacctgg tcaccgtccc cgatggcaaa
    43861 ttaagcccac gattggcggc actgaaaccg ggcgatgaag tgcaggtggt tagcgaagca
    43921 gctggcttct ttgttctgga tgaagtaccg gactgcgaaa cgctatggat gctggcaacc
    43981 ggtacagcga ttggccctta tttatcgatt ctgcaactag gcaaagattt agatcgcttc
    44041 aaaaatctgg tcctggttca cgccgcacgt tatgccgccg acttaagcta tttgccactg
    44101 atgcaagaac tggaaaaacg ctacgaaggc aaactgcgca ttcagacggt ggtcagtcgg
    44161 gaaacggcag cggggtcgct caccggacgg ataccggcat taattgaaag tggggaactg
    44221 gaaagcgcga ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct gtgcggcaat
    44281 ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat gacgaaacat
    44341 ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt tacttatcga
    44401 taaacggcac gatgagcaaa tccgcactca tcttattgat catcccgcga tatgccggca
    44461 tcaaacggtt gataaatgag tgatgatgac cacagacaag gaggtcgcac tgctcttttt
    44521 gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt tttgtcttcg
    44581 gccattgaat atttttcgtc agtttataca gcttgttatc cgacttattc ttcaacaatt
    44641 gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt aagccatcat
    44701 caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccagg gctttattca
    44761 ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta taagccatgt
    44821 ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact caaaaataat
    44881 catctcggcc tggcatgaga aagtgaaggc cgcatcaagc tcaacttgcc cctccagatc
    44941 cttaatctta taggccacgc tggccccttc atctgcagag gatgcagcgg cggcaagctc
    45001 tttcagtttg gtcataaaag actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt
    45061 actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc ctttagtact
    45121 gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc tgtcgattac
    45181 gacttaagaa taatgcgccg ttaagggaag aacaaacaag taatgatctt atggattacc
    45241 attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggt attgtcattt
    45301 gcatcgtgga aataacaatc ccggaaaaaa atagcagtaa gagatatatt aaaggttaat
    45361 tacttgattt attgtcggct ttatacttca catcctgagt atctttacca tatttatttt
    45421 cgccttgtgt gccaacaaac gcaccaagat cgataagcat catcaccaga atcaacgtcg
    45481 ggacaaaacg ccccaccgcc cattgccaga cacccggtaa aatcgctcag ttacccgcca
    45541 gcagcatcca cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca
    45601 agcgcttaac agttactgcc gctgttggcc agagcaagca cacaaggcaa aacgccgcgg
    45661 tctgaatatc gagtagattc ttacccgcca gtgagaaaag caccagcatg cctgcgaacc
    45721 acaggcctat ccaaatccag aaatcacggc gtccaatacg ccctttaaat gagaataacc
    45781 attgctgtat ggtcatgtaa gttccttgat ggttgtcttt tccaggattc tacccttttg
    45841 acaagggcga cagatatcgt tttaatcgga gccagtcata acaaaaggta ctgtcaatga
    45901 agccagggtg tacgctgttt tttctcttat gttctgcatt aaccgttaca acaacggcgc
    45961 atgcacaaac accagatacg gcaacgaccg cgccttatct gctggctgga gcccctactt
    46021 tcgatctctc catcagccag tttcgagaag actttaacag ccagaatccc agcctgccac
    46081 tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc caatctcact cgtgctgcaa
    46141 gtaaaattaa cgagaacttg tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca
    46201 aaagcattca aatgacctgg ctacccatcc aggggccaga gcaaaaagca gcgaaagcga
    46261 aagctcagga atacatggca gcagtgatcc gcacactcac cccattaatg accaaaacac
    46321 aaagccagaa aaaactgcag tcgctactaa cggcggggaa aaacaaacgt tattacaccg
    46381 agacagaagg tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg
    46441 ctgttgaacc gattaagctg gcgctatctg aatcgcttga aggtttgaat aaatgacaaa
    46501 aagcaaagcc tttgtgccga tgaatctcta tactgtttca cagacctgct gccctgcggg
    46561 gcggccatct tcctttattc gcttataagc gtggagaatt aaaatgcgac atcctttagt
    46621 gatgggtaac tggaaactga acggcagccg ccacatggtt cacgagctgg tttctaacct
    46681 gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca atcgcaccac cggaaatgta
    46741 catcgatatg gcgaagcgcg aagctgaagg cagccacatc atgctgggtg cgcaaaacgt
    46801 ggacctgaac ctgtccggcg cattcaccgg tgaaacttct gccgctatgc tgaaagacat
    46861 cggcgctcag tacatcatca tcggccactc tgaacgtcgt acttaccaca aagagtctga
    46921 cgaactgatt gcgaaaaaat tcgcggtgct gaaagagcag ggtctgactc cggttctgtg
    46981 catcggtgaa accgaagctg aaaacgaagc gggaaaaact gaagaagttt gcgcacgtca
    47041 gatcgacgcg gtactgaaaa ctcagggtgc tgcggcattc gaaggtgcgg ttatcgctta
    47101 cgaacctgta tgggcaatcg gtactggcaa atctgcaact ccggctcagg cacaggctgt
    47161 tcacaaattc atccgtgacc acatcgctaa agttgacgct aacatcgctg aacaagtgat
    47221 cattcagtac ggcggctctg taaacgcgtc taacgctgca gaactgtttg ctcagccaga
    47281 catcgacggc gcgctggttg gcggtgcttc tctgaaagct gacgctttcg cagtgatcgt
    47341 taaagctgca gaagcggcta aacaggctta agtctgacag gtgccggatt tcatatccgg
    47401 cacttacttt ccttaactcc tcgccttaac gcaaaatctc acactcatga tcctggattt
    47461 cctcggctga agcacggttc agcgtcagta gatttcgttg tgtcgccagc aacacaaacg
    47521 aattatcact ctgccgtacc atcgccagcc cgtagcttcc catatgttcc cgcgcctcag
    47581 gcacttcttc tgcaagcatc ataaatgggc tgcgttgtac cagttcgctt tccgttaccc
    47641 gacgcgccag gtattcatgc ccgcgcaaac cacctggcag tggcaaccag cggctgctga
    47701 tgttcgccag atttttatcc agttgttcgc gcacatcagg acgaatacaa gagatatgaa
    47761 tatgaaaatg gttttgcgta tgcccggtgc gggagttgat cgccaaagaa accgcgcgat
    47821 cgggaaccgg ctggccgtat tttttgctca taaaatcacg cgcctgccag gccaaccaaa
    47881 agaagttcgg cgttgaagga tcggtcaaca aaggactttc agtaccgtta atacgatacg
    47941 ttggcatcaa cagatattgc agtggaccat taagatcttt taaaaccacg tatccggcat
    48001 tgggtttgac ttccgcacat ggcgaaggat tttgattttg ctgctgattg ggcaaacatt
    48061 cctcaaggac aatcttacgt aatgtatccg actcttcacc ggttaatttc cagtaaccaa
    48121 taccggcagc cacaacggcg ataactatca tcaccaaaaa aagaagaccc gcttttttca
    48181 tctttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa
    48241 aacccggtcg cgagaaacca ccgggtttga taattatcct agggaaatca gcgtttgctg
    48301 atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg
    48361 aactcttcat caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgct
    48421 acttcagcgt cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag
    48481 tagagatatt tcaggtaggc ttccgccacc tctttggtgc cttttttctc gaccacttta
    48541 tcgaccaccg acaccgttgg ctctgcgagg atagactcac tcggcgtgac gatttcgaat
    48601 ttatctttcc ccagttcatt cgctgccagc agagcttcgt tttcccaggc gatcagtaca
    48661 tcgccaatcc cgcgctcgac aaaggtgtta gtggagccgc gcgcgccaga atccagaact
    48721 tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg
    48781 tgatgcagcg cgtagcccca ggcagccagg tagttccagc gtgcgccacc agagcttttc
    48841 ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc
    48901 ttcggattgc ccttacgcac caggaaaaca atggtggaag tgtacggtgc ggagttatcc
    48961 ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca
    49021 taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt
    49081 ttacctgaac caccgtgtga ctgacgaatc accacgttat caccagtttg ctgtttccag
    49141 tgggcgctga atgccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg
    49201 ttaagaagct gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct
    49261 acgccccact tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc
    49321 agaaggttaa ccaaacatta aagaataaaa aaagattggc tataacttgc gggtatatgt
    49381 tgagggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc
    49441 atgccggatg cggcgcgaac gccttatccg gcctacaaaa gtttgcaaat tcaataaatt
    49501 gcagaattca tgtaggcctg ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg
    49561 tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc
    49621 cagccagtca cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg
    49681 aaccagctgt tcgttctgga tacctacaca acgaccgccg taacctgcca gcagcagatc
    49741 gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc
    49801 gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa
    49861 atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccacgatcg cgtgtttttt
    49921 acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc
    49981 cggaactaca acgaattcac agccaccggc aatggccgca gccagggtca gatcgccaca
    50041 ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag
    50101 acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt
    50161 gcctttgatg tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc ccatttcggt
    50221 cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatacc
    50281 acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa
    50341 acgcgcagaa ccgaggaacg taccgccacg gttgatcatg tcagacacgc tgtaacggtc
    50401 tagctgtacc atacggtctt catacagacc cagatagccg tcataaatac ccattacttc
    50461 cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc
    50521 atcaccgccg cttgtcaaca cacctatttt cttaatcatg actacctctg aactttggaa
    50581 tgcaaaatga aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg
    50641 caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt
    50701 ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt
    50761 accctccctg ggtacgacgg aacaggggtc ctgatggata atcacatccg atcccggaaa
    50821 acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag
    50881 agagtcttcc atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg
    50941 aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg
    51001 ttcttcatca ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa
    51061 cgcgctatat aaaatataga tgccgattcc caatgcaaac agagcatcgg cgcgatgcca
    51121 gccgtaccaa gacaacccca gcgccagcag aattgcgccg ttcatcataa catcagactg
    51181 gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cggcgcacca cccaccgctg
    51241 aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc
    51301 tggatctgtc atcggtgttg gagataccag atgttgaata cccgtcaaaa acaggaatag
    51361 tgccgaaccg gagataaaca tactttgcgc cagcgccgcg agggactcag ctttaccgtg
    51421 accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca gtaaattcgt
    51481 caacgacgcg ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc
    51541 ggtataccac catgcaaaaa ttttaatcag caatagcaac gaagccatcg ccgtcgcagc
    51601 aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa
    51661 ctgacgctag tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag
    51721 gataaaaaaa accccacatc atgtggggga agacagggat ggtgtctatg gcaaggaaaa
    51781 cagggtttac tactgggaac gtgagttgct actactcaat agcttcaacg atgaactttt
    51841 ttgccattgc gtcacgtcac gcaactgctc cattcgttgt tgatgtttct cgtttaaaac
    51901 cgcttgctgc tccggcgtta acaggcgata catttggttg cggacttttg ccatctcaac
    51961 ctgacgagca atttgctcat tcgccatttt ttctgcctgt gcgcgcacag cgttttcatc
    52021 aaaattttct gcggtgacaa ggcgatgcat tgtctccagt tcgctaacat taacaggagg
    52081 ctgttcgtgc cgggcctgtt gcataagatc tcgcatctgc tgacgctgat gttcggttaa
    52141 acttatgccg tcgaacatat ggctctgcgt actgcgctgc gtaagttctt cacccggatg
    52201 ccagttatcg cctgaaccga cttcagcagc gtggcttaat gaactgactg ccagcgttga
    52261 ggccatgacg gcagcggtaa ctatgcgcat catttgctcc caaaatcttt ctgtcgcgat
    52321 tcaacgatag agagtttacg attcaggctg caaacatgcg tcagggggtg taaaacaacg
    52381 taaagtcatg gattagcgac gtctgatgac gtaatttctg cctcggaggt atttaaacaa
    52441 tgaataaaat cctgttagtt gatgatgacc gagagctgac ttccctatta aaggagctgc
    52501 tcgagatgga aggcttcaac gtgattgttg cccacgatgg ggaacaggcg cttgatcttc
    52561 tggacgacag cattgattta cttttgcttg acgtaatgat gccgaagaaa aatggtatcg
    52621 acacattaaa agcacttcgc cagacacacc agacgcctgt cattatgttg acggcgcgcg
    52681 gcagtgaact tgatcgcgtt ctcggccttg agctgggcgc agatgactat ctcccgaaac
    52741 cgtttaatga tcgtgagctg gtggcacgta ttcgcgcaat cctgcgccgt tcgcactgga
    52801 gcgagcaaca gcaaaacaac gacaacggtt caccgacact ggaagttgat gccttagtgc
    52861 tgaatccagg ccgtcaggaa gccagcttcg acgggcaaac gctggagtta accggtactg
    52921 agtttaccct gctctatttg ctggcacagc atctgggtca ggtggtttcc cgtgaacatt
    52981 taagccagga agtgttgggc aaacgcctga cgcctttcga ccgcgctatt gatatgcaca
    53041 tttccaacct gcgtcgtaaa ctgccggatc gtaaagatgg tcacccgtgg tttaaaacct
    53101 tgcgtggtcg cggctatctg atggtttctg cttcatgata ggcagcttaa ccgcgcgcat
    53161 cttcgccatc ttctggctga cgctggtgct ggtgttgatg ttggttttga tgttacccaa
    53221 gctcgattca cgccagatga ccgagcttct ggatagcgaa cagcgtcagg ggctgatgat
    53281 tgagcagcat gtcgaagcgg agctggcgaa cgatccgccc aacgatttaa tgtggtggcg
    53341 gcgtctgttc cgggcgattg ataagtgggc accgccagga cagcgtttgt tattggtgac
    53401 caccgaaggc cgcgtgatcg gcgctgaacg cagcgaaatg cagatcattc gtaactttat
    53461 tggtcaggcc gataacgccg atcatccgca gaagaaaaag tatggccgcg tggaactggt
    53521 cggtccgttc tccgtgcgtg atggcgaaga taattaccaa ctttatctga ttcgtccggc
    53581 cagtagttct caatccgatt tcattaactt attgtttgac cgcccgctat tactgctgat
    53641 tgtcaccatg ttggtcagta cgccgctgct gttgtggttg gcctggagtc tggcaaaacc
    53701 ggcgcgtaag ctgaaaaacg ctgccgatga agttgcccag ggaaacttac gccagcaccc
    53761 ggaactggaa gcggggccac aggaattcct tgccgcaggt gccagtttta accagatggt
    53821 caccgcgctg gagcgcatga tgacctctca gcagcgtctg ctttctgata tctctcacga
    53881 gctgcgcacc ccgctgacgc gtctgcaact gggtacggcg ttactgcgcc gtcgtagcgg
    53941 tgaaagcaag gaactcgagc gtattgaaac cgaagcgcaa cgtctggaca gcatgatcaa
    54001 cgatctgttg gtgatgtcac gtaatcagca aaaaaacgcg ctggttagcg aaaccatcaa
    54061 agccaaccag ttgtggagtg aagtgctgga taacgcggcg ttcgaagccg agcaaatggg
    54121 caagtcgttg acagttaact tcccgcctgg gccgtggccg ctgtacggca atccgaacgc
    54181 cctggaaagt gcgctggaaa acattgttcg taatgctctg cgttattccc atacgaagat
    54241 tgaagtgggc tttgcggtag ataaagacgg tatcaccatt acggtggacg acgatggtcc
    54301 tggcgttagc ccggaagatc gcgaacagat tttccgtccg ttctatcgga ccgatgaagc
    54361 acgcgatcgt gaatctggcg gtacaggatt ggggctggcg attgttgaaa ccgccattca
    54421 gcagcatcgt ggctgggtga aggcagaaga cagcccgctg ggcggtttac ggctggtgat
    54481 ttggttgccg ctgtataagc ggagttaaac tccgcatttg taggcaggat aaggcgttta
    54541 cgccgcatcc ggcatttgag caggatgcct gatgcgacgc tgatagcgtc ttatcaggcc
    54601 tacactccgg gggttatttt ccccacaacc ggcgggaaaa atcttcgatc ttgccgctca
    54661 gtcggcgctt ttgcatcgtc cgcgtccagc ttttcgataa cccggcggcg gagagtaaac
    54721 ggtgatactg atcgtcatca aacggcatat gccatgcgat ggcggcagcc tcctgcacgg
    54781 tcacatcgct gacacgggaa accaactcca gcggcgcgtc cgcagacact ttccctggcg
    54841 ctatcacgct atacaaccac cccaccttac ctgtgttttg catcaactgc gctatatcgc
    54901 tgatatcaaa atggtaattg agtttatagc aaggcgagcg cggctggctg acctgaatta
    54961 atgcctctcc ccagcggaaa atatcgccca tatgaacatt actttccgtg agaccgtcgg
    55021 ttgagagatt ttcgccaaac gcaggcgcaa caaacaactc cgcctgttcc ggaaattccc
    55081 gcacccagta gagatagtgc tcacgaggat aatgacacag cgctctgtct ggcccgccgt
    55141 gaaccttttt ctccgcctgc tcgtcacctt ccagccccag ctccgtcagc atcaactcac
    55201 catcaacctg gattttggca attgcgctgg gtttgccttc gggataagcc tgaatcttgc
    55261 ctgtgtatac atcaaccgga tatcgcatct gttccctctc cacgagaaat ttccccatta
    55321 gaccatattt attttatgtc agccgtgctg ttttcgcggt aagtatccag gcaggaactc
    55381 aatagcaaac cggacgaacg ttagttcatc cggctgtggg g
//
LOCUS       DAJVQR010000017        53203 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVQR010000017 DAJVQR010000000
VERSION     DAJVQR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(11..322)
                     /locus_tag="OQM52_001069"
     CDS             complement(11..322)
                     /locus_tag="OQM52_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1563010.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OQM52_001070"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQM52_001070"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OQM52_001071"
     CDS             701..1054
                     /locus_tag="OQM52_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1563011.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OQM52_001072"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OQM52_001072"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1563012.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OQM52_001073"
     CDS             complement(2810..3280)
                     /locus_tag="OQM52_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS1563013.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OQM52_001074"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OQM52_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OQM52_001075"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OQM52_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1563014.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OQM52_001076"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OQM52_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1563015.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5282..8431)
                     /locus_tag="OQM52_001077"
     CDS             complement(5282..8431)
                     /locus_tag="OQM52_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1563016.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="OQM52_001078"
     CDS             complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="OQM52_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1563017.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9789..10436
                     /gene="acrR"
                     /locus_tag="OQM52_001079"
     CDS             9789..10436
                     /gene="acrR"
                     /locus_tag="OQM52_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1563018.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10564..13926
                     /gene="mscK"
                     /locus_tag="OQM52_001080"
     CDS             10564..13926
                     /gene="mscK"
                     /locus_tag="OQM52_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1563019.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="OQM52_001081"
     CDS             complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="OQM52_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1563020.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14313..14840)
                     /gene="priC"
                     /locus_tag="OQM52_001082"
     CDS             complement(14313..14840)
                     /gene="priC"
                     /locus_tag="OQM52_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1563021.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14910..15287
                     /locus_tag="OQM52_001083"
     CDS             14910..15287
                     /locus_tag="OQM52_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1563022.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15440..15991
                     /gene="apt"
                     /locus_tag="OQM52_001084"
     CDS             15440..15991
                     /gene="apt"
                     /locus_tag="OQM52_001084"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1563023.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16120..18045
                     /gene="dnaX"
                     /locus_tag="OQM52_001085"
     CDS             16120..18045
                     /gene="dnaX"
                     /locus_tag="OQM52_001085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1563024.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17378..17442
                     /gene="dnaX"
                     /locus_tag="OQM52_001085"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18098..18427
                     /locus_tag="OQM52_001086"
     CDS             18098..18427
                     /locus_tag="OQM52_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1563025.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18427..19032
                     /gene="recR"
                     /locus_tag="OQM52_001087"
     CDS             18427..19032
                     /gene="recR"
                     /locus_tag="OQM52_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1563026.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19142..21016
                     /gene="htpG"
                     /locus_tag="OQM52_001088"
     CDS             19142..21016
                     /gene="htpG"
                     /locus_tag="OQM52_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1563027.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21197..21841
                     /gene="adk"
                     /locus_tag="OQM52_001089"
     CDS             21197..21841
                     /gene="adk"
                     /locus_tag="OQM52_001089"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1563028.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21973..22935
                     /gene="hemH"
                     /locus_tag="OQM52_001090"
     CDS             21973..22935
                     /gene="hemH"
                     /locus_tag="OQM52_001090"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1563029.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22932..23891)
                     /gene="aes"
                     /locus_tag="OQM52_001091"
                     /pseudo
     CDS             complement(22932..23891)
                     /gene="aes"
                     /locus_tag="OQM52_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24043..25347
                     /gene="gsk"
                     /locus_tag="OQM52_001092"
     CDS             24043..25347
                     /gene="gsk"
                     /locus_tag="OQM52_001092"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1563030.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25480..27156)
                     /locus_tag="OQM52_001093"
     CDS             complement(25480..27156)
                     /locus_tag="OQM52_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1563031.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="OQM52_001094"
     CDS             complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="OQM52_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1563032.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28832..30484
                     /gene="ushA"
                     /locus_tag="OQM52_001095"
     CDS             28832..30484
                     /gene="ushA"
                     /locus_tag="OQM52_001095"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1563033.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="OQM52_001096"
     CDS             complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="OQM52_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1563034.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31204..31998)
                     /locus_tag="OQM52_001097"
     CDS             complement(31204..31998)
                     /locus_tag="OQM52_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1563035.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32136..32477
                     /locus_tag="OQM52_001098"
     CDS             32136..32477
                     /locus_tag="OQM52_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1563036.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32692..35196)
                     /gene="copA"
                     /locus_tag="OQM52_001099"
     CDS             complement(32692..35196)
                     /gene="copA"
                     /locus_tag="OQM52_001099"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1563037.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35458..36390
                     /gene="glsA"
                     /locus_tag="OQM52_001100"
     CDS             35458..36390
                     /gene="glsA"
                     /locus_tag="OQM52_001100"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1563038.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36393..37685
                     /locus_tag="OQM52_001101"
     CDS             36393..37685
                     /locus_tag="OQM52_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1563039.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37810..38217
                     /gene="cueR"
                     /locus_tag="OQM52_001102"
     CDS             37810..38217
                     /gene="cueR"
                     /locus_tag="OQM52_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1563040.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38218..38676)
                     /locus_tag="OQM52_001103"
     CDS             complement(38218..38676)
                     /locus_tag="OQM52_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1563041.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38673..39590)
                     /locus_tag="OQM52_001104"
     CDS             complement(38673..39590)
                     /locus_tag="OQM52_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1563042.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39736..40413
                     /gene="fetA"
                     /locus_tag="OQM52_001105"
     CDS             39736..40413
                     /gene="fetA"
                     /locus_tag="OQM52_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1563043.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40400..41179
                     /gene="fetB"
                     /locus_tag="OQM52_001106"
     CDS             40400..41179
                     /gene="fetB"
                     /locus_tag="OQM52_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1563044.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="OQM52_001107"
     CDS             complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="OQM52_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1563045.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="OQM52_001108"
     CDS             complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="OQM52_001108"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1563046.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="OQM52_001109"
                     /pseudo
     CDS             complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="OQM52_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43549..44235
                     /locus_tag="OQM52_001110"
     CDS             43549..44235
                     /locus_tag="OQM52_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1563047.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44232..46647
                     /locus_tag="OQM52_001111"
                     /pseudo
     CDS             44232..46647
                     /locus_tag="OQM52_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OQM52_001112"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OQM52_001112"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1563048.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OQM52_001113"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OQM52_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS1563049.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="OQM52_001114"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="OQM52_001114"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCS1563050.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="OQM52_001115"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="OQM52_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCS1563051.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="OQM52_001116"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="OQM52_001116"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCS1563052.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53203
                     /locus_tag="OQM52_001117"
     CDS             52938..>53203
                     /locus_tag="OQM52_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1563053.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAA"
BASE COUNT        12827 a        13689 c        14014 g        12673 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaggcc gcttacgcgg ccttagtgat tacatgttgt
     5281 atcaatgatg atcgacagta tggctgtgct cgatatcttc attcttgcgg ctaaagcggc
     5341 ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt gcggtcacca
     5401 tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca ccagtactga
     5461 taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc ggacgtaaac
     5521 gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta tccatcaagt
     5581 ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg gttgtgagca
     5641 gtcctacttg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc agcaacgcac
     5701 cgataacccc cagcggaacg accagcataa cggagaacgg agtcgaccag ctctcgtaca
     5761 gcgccgccag acacaggaat acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt
     5821 tgccggagag acgttcctga taggacatcc ccgtccagtc atagccaaca ccggtaggca
     5881 gtttgctcgc cagttgttcc atcagctcca ttgcttcacc ggtactttta cccggtgccg
     5941 cctggcctaa gatttccatg gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt
     6001 actcccaacg agaagaggag aacgccgaga atggcaccat ctgaccatca gcagcacgaa
     6061 cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac atgacgtaaa
     6121 ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctaccgccc catgcagcgc
     6181 ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga
     6241 tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg gtcagcatat
     6301 cagggtgctt cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt ttttcgtgac
     6361 caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcagtaccc agttccacga
     6421 ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg
     6481 cacgcatggt aatcgcttca actttgtttt cttcgcccgg acgatcggcc cagtccttca
     6541 aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag ccgttaacgg
     6601 cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt acctcattga
     6661 gcactttctg cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg gtcataaata
     6721 cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacgaacaga taggccatgc
     6781 cgaccacgat gatcagatac agcactaggt aacgccccgt actgcgcaga ataccgccta
     6841 cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttcaaccag ccgaagaagc
     6901 cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg gcacaaagag
     6961 ccggagtcag gatcaacgct accagtaccg acagcgccat tgctgaaaca atggtaatag
     7021 agaactgacg atagatagca ccagtagaac cgccaaagaa ggccatcggt acgaataccg
     7081 ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc gacttacggg
     7141 tagcttcttt tggcggcaaa ccttcttccg ccataacacg ctccacgttt tctaccacaa
     7201 cgatagcgtc atccaccaac aagccgatgg cgagcaccat cccgaacatt gttagcgtgt
     7261 ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc accggtacgg
     7321 caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacata accaggaaca
     7381 cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt ttcacgaacg
     7441 gcgtggtgtc gtacgggtaa acaattttca gacccgacgg gaagaacggt tccatcttcg
     7501 ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga
     7561 tccccagacc ggaagcaggt tggccgttaa actctgcgat gatgtcgtag ttctcaccac
     7621 ccagctcaat cttcgccaca tcacgcagca gtacgcggga accatcctga ttcactttca
     7681 gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata atagaggcgt
     7741 taagctgttg gcctttcacc ggcggcgtac caccgagctg acccgccgca acctgggcgt
     7801 tctgcgcttt gatggcggta atgacatcaa ccggcgttag ctggaatttg ttcagctcat
     7861 tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaaca tcacccacgc
     7921 ccgacgtacg gctgatggca tctttcatat tcgccgctac gtagtcagag atatcctcct
     7981 gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg ctggatgatt
     8041 tctcaacgct caccccttgc tgctgaactt cttgcggcag caacggcatc gccagctgca
     8101 gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca aaggtcaggg
     8161 tgatctgcac ggtacccgtg gagtcactgt tagaggacat gtacatcagg ttatcgatac
     8221 cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc
     8281 cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat tgcgccaccg
     8341 gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc acccacgcaa
     8401 aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt aagttaagac
     8461 ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc ggtaacttct
     8521 tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac tacgcgatcg
     8581 cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct tgcaacgatc
     8641 ggacgggttt ccactttgtc atccgcgcca actaccagta cggtggcatc accacgcggc
     8701 gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc ttcttccaga
     8761 cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt
     8821 agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc
     8881 tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc gttctcttgt
     8941 ttcagcgtgc cattcgccag ttcctgtttc aggcgaagga agtcgttgct ggactgggtc
     9001 acatcaacgt agatcggatc aagttgctgc acggttgcca gcacagtcgc ctgaccgttc
     9061 tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat cggagaggtg
     9121 actttggtgt aagccagatt gatccgcgca gtttcaacgg cagctttcgc cgcagttacc
     9181 gcagcattcg cctgttgcgc atcagccaga gcctgatcgt acccttgctt actgatgtac
     9241 tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt ggctgcagcc
     9301 tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt cgcaggatca
     9361 atctgataga gagagacacc agcttcgatg tcgctacctt ctttgaaatt acgcttcagg
     9421 ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt gcgacctgga
     9481 agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac ggcgggcatc
     9541 tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag
     9601 agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg taaacctcga
     9661 gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct gcgtttatat
     9721 tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc ctgtaaattc
     9781 acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa cacatcctcg
     9841 atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg ggcgagattg
     9901 caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac aagtcggatt
     9961 tgttcagtga gatctgggaa ctgtcagaat ccaatattgg tgaactagag cttgagtatc
    10021 aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt catgttcttg
    10081 aatccacggt gacagaagaa cgacgtcgat tattgatgga gattatattc cacaaatgcg
    10141 aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt ctggaaagtt
    10201 atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt
    10261 taatgacgcg tcgggcagca attattatgc gcggctatat ttccggcctg atggaaaact
    10321 ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac gtcgccatct
    10381 tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac gaataaccct
    10441 gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg ttcactgcgt
    10501 cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa accttcattc
    10561 atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc agcatttatt
    10621 gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc atcgaatggt
    10681 gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa acaaaaagat
    10741 ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc caccctcgat
    10801 aaaatcgatc gcataaaaga agagacagtt cagctacggc aaaaagtcgc tgaagcgccg
    10861 gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga taacgacgaa
    10921 gaaacgcgca aaattctgag tacgctttcg ttgcgccagc tggaaactcg cgttgcccag
    10981 gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag ccagctggtt
    11041 tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg
    11101 caacaaattc gcagtcgtct ggatgggact gatgtcggcg agactgcctt acgtcccagc
    11161 cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg cggagattga ccagcagcgt
    11221 aaaagcctgg aagggaacac cgtcttgcag gataccttgc aaaagcaacg tgattacgtg
    11281 acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc ggtaaacagc
    11341 aagcgcctga ctttaactga aaaaacggcg caggaagccg tctccccgga tgaagccgcg
    11401 cgtattcagg ctaatccgct ggtgaaacag gaactggaaa ttaaccagca gttaagtcag
    11461 cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat taaagtcaaa
    11521 aactggctgg agcgggcgct gcaatcggaa cgcaatatta aagagcagat tgccgtcctg
    11581 aagggcagcc tgctgttgtc tcgtatcctt taccagcaac aacaaacgct gccctcggcg
    11641 gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt
    11701 aaccagcagc gtgatgcact cttccagagc gatgcgttcg tcaacaaact ggaagaaggt
    11761 cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt
    11821 cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat ggccattaac
    11881 ctgcaaatca accagcagca gttaatgagc gtatcgaaaa acctgaaatc catcctgact
    11941 cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat caaagcgttc
    12001 ccgcaaagcc tgaaagatga atttaagtcg atgaaaatca cggtgaactg ggaaaaagcc
    12061 tggcctgccg tttttatcgc tttcctcgcc ggtttgccgc tgctgttgat tgccgggctg
    12121 atccactggc gtctgggctg gctgaaagcg tatcaacaga aactggcttc cgctgtgggt
    12181 tccctgcgta acgatagcca gctcaacaca ccaaaagcga tccttatcga cctgatccgc
    12241 gcgctgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac catgcagctc
    12301 aacatcagcg aactgctatg gtcgttcagt aaaaaactgg cgattttctg gctggtgttt
    12361 ggcctgtgct ggaaggtact ggagaaaaat ggcgttgccg tgcgtcactt cggcatgccg
    12421 gagcagcaga ccagccactg gcgtcggcaa attgtccgca tcagtctcgc gttgctgcct
    12481 atccatttct ggtctgtggt ggcagaactt tccccgctgc atctgatgga tgatgtgctg
    12541 gggcaggcga tgattttctt caacctgctg ctgattgcct tcctggtatg gccaatgtgc
    12601 cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tagtcaccat taccgtgctg
    12661 tcgattatcc cgattgcatt gatggtgctg actgccacgg gttatttcta caccacgctg
    12721 cgcctctccg ggcgttggat tgaaaccgtt tatctggtga tcatctggaa cttgctctac
    12781 cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg
    12841 gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc ggaagaaccc
    12901 accattgcac tggagcaagt taaccagcag acgctgcgta ttaccatgtt gctgatgttt
    12961 gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt gttcagctat
    13021 ctcgacagca tcacgctctg gcattacaac ggcactgaag ctggcgctgc ggtggtgaaa
    13081 aacgtcacca tgggcagtct gttgtttgcg attatcgcct caatggtggc ctgggcgttg
    13141 attcgcaacc tgcctggttt actggaagtg ctggtgctct cgcgactgaa tatgcgccag
    13201 ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgccgt tggtgcgatg
    13261 acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc cgcagcatta
    13321 tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc cggtttgatc
    13381 attctgttcg aacgtccggt gcgtattggc gatacggtaa ccattggtag cttctcgggg
    13441 acggtaagta agatccgtat tcgtgcgaca acgattaccg atttcgatcg caaagaagtg
    13501 atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt gactgacact
    13561 actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt
    13621 aaagtgttac tgaaggcggc gactgagcac ccaagggtga tgcacgaacc aatgccggaa
    13681 gtcttcttta cggcatttgg tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt
    13741 gaactgcgtg accgtagccg tactgtcgat gagctgaacc gtactatcga tcagctgtgc
    13801 cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca caacgagaag
    13861 ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg acgatccgac accagcggtt
    13921 gggtaataac caaagggggc gacatttagt tgccccgaga ttactgacaa agtgcgcgtt
    13981 gttcatgccg gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa gaattcaata
    14041 tattgcagga gcggtgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgccc
    14101 gcaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgt
    14161 gcgttaattt cttttcataa tcacgcttta caatatccag cgcgcgcagc acggtactgg
    14221 cagggatctg attttcctcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
    14281 gggcattttc aagtgacata ctctttccat tgctagcggg ttaagcgcgc tagcctgttt
    14341 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
    14401 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
    14461 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
    14521 cgggaaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
    14581 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
    14641 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcgtc gagacaagac
    14701 tgtagtgttg tcgcacgagt ctgaaaaaga tgcctgtcga aacgagcact tagcgtggcg
    14761 aactgtgaca ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
    14821 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
    14881 agccactatg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
    14941 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
    15001 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
    15061 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
    15121 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
    15181 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
    15241 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
    15301 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
    15361 gtcaaaggtt aaacaacagt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
    15421 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
    15481 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
    15541 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
    15601 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
    15661 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
    15721 aaccatcagt gaaaattacg acctggaata cggcaccgat cagctggaaa tccacgttga
    15781 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
    15841 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
    15901 cattatcaac ctgtttgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
    15961 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
    16021 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
    16081 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggttttagcc
    16141 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
    16201 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt
    16261 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
    16321 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
    16381 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
    16441 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
    16501 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
    16561 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
    16621 tcacgctgtc tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcagctt
    16681 gagcacatcc tcaatgaaga acatatcgct cacgagccgc gggcgctgca attgttggcg
    16741 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
    16801 ggtgacggcc aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat
    16861 caggcgctgt cgctggttga agcgatggtc gagggcaacg gcgagcgcgt aatggcactg
    16921 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
    16981 ttgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
    17041 gccatcgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
    17101 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
    17161 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cgcgtatgcc gctgcctgag
    17221 ccagaagtgc cacgccagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc
    17281 ctggtgccgc cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    17341 agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    17401 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    17461 ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg
    17521 ccagccaaaa aagaagcgta tcgctggaag gcgaccactc cggtgatgca gcaaaaagaa
    17581 gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    17641 gcggtgaagc tagcggcaga agccattgag cgcgacccgt gggcggcaca ggtgagccaa
    17701 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    17761 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag
    17821 caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatcgtt
    17881 gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa
    17941 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcgg
    18001 tttttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt
    18061 acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    18121 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    18181 cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    18241 tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    18301 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    18361 gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    18421 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg
    18481 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc
    18541 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    18601 gattgccgca ctttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag
    18661 gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca tctacgccat tgagcagacg
    18721 gggcagtttt ctggtcgtta ttttgtgttg atggggcatc tgtcaccgct ggacggcatc
    18781 ggtccggatg atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    18841 gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag ctaccgctaa ctacattgcc
    18901 gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    18961 ggcgaactgg aaatggtcga tggcaccacg ctgtcacact ccctcgccgg gcgtcataag
    19021 attcgttttt aagcaaacga gagcaggagc acctgctctc gcttgaaatt attctccctt
    19081 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    19141 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    19201 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    19261 cgatgcggcg gacaagctgc gtttccgtgc gctatctaac ccggacctgt acgaaggtga
    19321 tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    19381 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    19441 cggtaccaaa tcattcctcg aatccctggg ttctgaccaa gcgaaagaca gccagctgat
    19501 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg
    19561 tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg
    19621 tgaatatacc gttgccgata tcaccaaaga agatcgtggt actgaaatca ccctgcatct
    19681 gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata
    19741 ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag atggcgaaac
    19801 cgttatctcc tgggcaaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga
    19861 aatcaccgat gaagagtata aagagttcta caaacacatc gcccacgact ttaacgatcc
    19921 gctgacctgg agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat
    19981 cccgtcccag gctccgtggg atatgtggaa ccgtgatcat aaacacggcc tgaaactgta
    20041 cgttcaacgc gtgtttatca tggacgacgc tgaacagttc atgccgaact atctgcgctt
    20101 cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    20161 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    20221 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    20281 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct
    20341 gcgttttgct tctacttata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    20401 ttcccgcatg aaagaagggc aggagaaaat ctactacatc actgcagaca gctatgcggc
    20461 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    20521 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    20581 ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    20641 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgcgtgaaag ctctgctcgg
    20701 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc
    20761 gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa
    20821 agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    20881 ggcagatact gaagatgaag ccaagttcag cgagtgggta gaactgctgc tggatcaggc
    20941 gctgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaatca
    21001 gctgctggtt tcctgatgtc atgccggatg acctttgtgt catccggcat ttttcttttc
    21061 atcatctgta ctttccgcaa attatctcgc cattaaccgt ttcagcctca ggtgcctttc
    21121 ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaaat cgacacattt
    21181 taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac
    21241 tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct
    21301 gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc
    21361 tggcaaactg gtcaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga
    21421 agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc
    21481 gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact
    21541 gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg tctggtcgtg tttatcacgt
    21601 taaattcaat ccgccgaaag tagaaggcaa agacgacgtt accggtgaag aactgactac
    21661 ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg gttgaatacc atcagatgac
    21721 tgcaccgctg atcggctact actccaaaga agcggaagcg ggtaacacca aatacgcgaa
    21781 agttgacggt accaagcagg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta
    21841 atcaagccgc agcagatctg ctttcgggca ggtctgctta tccacctcag gcaatacctc
    21901 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    21961 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    22021 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    22081 gtggttgata cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg
    22141 cgctcgccgc gtgtggcgaa gctgtacgcc tctgtctgga tggaagacgg ctcgccgctg
    22201 atggtttaca gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgccagtg
    22261 gcgctgggca tgagctacgg ctcgccatca ctggaaagct ccgtcgatga actcctggca
    22321 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc
    22381 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    22441 tcgtttattc gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    22501 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    22561 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc
    22621 gaactggctt ccgcactggg gatggtaccg gaaaaagtga tgatgacctt tcagtcgcgc
    22681 tttggccggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    22741 aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    22801 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgcgggcgg gaaaaaatat
    22861 gaatatattc cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt
    22921 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    22981 gcggttttca tcatccgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg
    23041 aactcacagg gctgctgatg cgccgctaac gtctagtaaa gcagacggct gtcatccagc
    23101 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    23161 ttaaacagac agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    23221 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    23281 acggaatccc gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa
    23341 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    23401 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    23461 taacaacaag cagccacaat ttcctctatc gcttgtggaa aacgcgcttc aggtgaaagg
    23521 gtgtaattaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    23581 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc
    23641 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    23701 atgtaagctc tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    23761 taatactgtc gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga
    23821 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt
    23881 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    23941 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    24001 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    24061 cgtaaatcca aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag
    24121 ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt
    24181 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    24241 gtgattgagg atgacgtagc cgaagtgctt tatcaggagc taaaacagaa aaacctgatt
    24301 acccatcagt ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg
    24361 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc
    24421 tatcgttacc tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat
    24481 ggtccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    24541 agtccaggcc acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga
    24601 gcctcggcac tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg
    24661 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    24721 ctgggcacca agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac
    24781 cacgtttcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    24841 ccgttgttgg catctgacaa ggcgctggac tgggtagagc tggtgctgtg caccgccggg
    24901 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    24961 ccgctgctgc cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc
    25021 cacaaggatt gccagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg
    25081 ccggaaaaaa tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat
    25141 gacattaccg ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc
    25201 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    25261 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    25321 caggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    25381 cacgccgcat ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg
    25441 cctacatatt tttcatattt tacatccggc aaccaccgtt taccccgtca ccacttcacc
    25501 cgccggtggc gtttccagca gttccagcat agtacgggca atttcacgtt cacccatcac
    25561 tacctgattc gcgccacgtt cggtgatata cgtcacttca tcgtcataat gggcgcgggc
    25621 gataatctca atatccgggt ttttcgcgcg ggcagacgcc acaatctcac ccgcttcata
    25681 accgttgggg atcgtcagga tcagccattt tgcacattcc agatgtgcca gttgcataat
    25741 ttcttcgttc gctgcattgc ccaatacagc gcggacccca cgctctcgca gctcatcaac
    25801 acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc
    25861 cagcaggctg cctacacgac cgtaacccac caatagcgca tggttgcaaa tatccactgg
    25921 gatctgtttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt
    25981 cgccagatat ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc
    26041 ccctgccagt accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc
    26101 cgccaggata aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt
    26161 acgttgggag tgaccaaaca gacggacaag gaacagggcc gcgaccgatt taccaaacag
    26221 aataattgcc agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa
    26281 caacatcccg actgagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcaacgtatc
    26341 gtgggcggca cggtgactca attcagactc gttcagtacc atcccggcaa agaacgcacc
    26401 gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc
    26461 cagcactgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca
    26521 cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca ccttgccgat
    26581 ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc
    26641 tgccactgcg ggcagcaaca ccagcgtcag aaccatcacc aggtcttcca caatcaacca
    26701 accgatggcg atttgcccac gctgactgtc tattaattgc cgttcttcaa gtgcgcgcag
    26761 taacaccacg gtactggcgg tggaaagaca taagccgaac acgataccgg tcattaacga
    26821 ccagcccagc acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat
    26881 cgcgccggga atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc
    26941 gacgccgaac atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc
    27001 ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag
    27061 aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc
    27121 aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta
    27181 ttgtccattt ttggctggga aaaccaaaat tacaggtaat agtttatgac aatttcattg
    27241 atgatgttta tgaataattg ttgaattttg cagaaaaatg gaattagctg caaaaaaagc
    27301 acggttcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga
    27361 tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag
    27421 gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat
    27481 gctggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca tgccgaaagc
    27541 aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagtagct cctgagcgta
    27601 gaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc
    27661 cgtccagtgc aggcgggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga
    27721 gccccaaatc acatatttcc gtccaatttt atcccctaca ggcccgccga tcaccgtacc
    27781 tgccgcaacg gcaaacagga aggcaaacag atgaagctgg gcattctgga tagataatcc
    27841 gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt
    27901 cgagaaaatg aggattaaca gaatgctgac tgccagtacc actttgttgc gcggcagtgg
    27961 attgattatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg
    28021 gctaatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt
    28081 gcctttgcca taaggctcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa
    28141 gttgccgccg acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg
    28201 ggctacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc
    28261 cagcagaact gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa
    28321 gcacatgcca attggcaacg accacggcat cggatattta tcggtccagt agccgaccac
    28381 tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa
    28441 tgtcagagaa aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat
    28501 gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt
    28561 ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt tcactcattg ccataggaaa
    28621 gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa
    28681 catacctgta tgcgtcgtct gaaggaagtc tcaacgccga atacagaatt tctaatctgg
    28741 atgcagattt atcttcaccg gacgcagact tgtctatgat gttgcgtcat actatttttc
    28801 aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt
    28861 agcgctgtta accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa
    28921 aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga
    28981 atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt
    29041 tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc
    29101 cgagtctgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga
    29161 cgcgatggcg atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga
    29221 aaagtgggcc aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg
    29281 cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg ttattgggct
    29341 gacaaccgat gacacagcaa aaattggtaa cctggaatac ttcactgata tcgaatttcg
    29401 taagcccgcc gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga
    29461 cattattatc gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc
    29521 accgggcgat gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg
    29581 tggtcactcg caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt
    29641 gccgggtacg ccatgcaaac cagatcaaca aaacggcatc tggattgtgc aggcgcatga
    29701 gtggggcaaa tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt
    29761 taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag
    29821 cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc
    29881 accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcaaaa ccaatggtcg
    29941 tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct
    30001 ggcagcccaa atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg
    30061 tgattctatc gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg
    30121 caatgtggtg gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt
    30181 cgcgcagatg aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc
    30241 gaaagacggc aaactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac
    30301 ttaccgtatg gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga
    30361 taacaaaccg ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat
    30421 ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca
    30481 gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat
    30541 cagcaaattt ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc
    30601 cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg
    30661 ttggcagacg ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac
    30721 gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt
    30781 ttttaagatc cagttgacct gcgaccggcg taacggccac ggcaaggtgt ttcatatcac
    30841 cgttcactgc caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga
    30901 cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg
    30961 aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa
    31021 caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta
    31081 agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga
    31141 cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt
    31201 tttttagcgc aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc
    31261 gaccacatat cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg
    31321 atgcatcagc acatcgtaca gcgactgact gaacgtattg ggcagcgtta tatcattatt
    31381 ttgcggcggt gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg
    31441 ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat
    31501 ggcaatctgg ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc
    31561 ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt
    31621 cgcctgaaga accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat
    31681 gcccatctcc tggctaatgt gctgcaggtt ttggagttgc tcctcgctaa tgcgctcttc
    31741 cagcgcctcg caggcaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc
    31801 gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat
    31861 atcgtggcta cccatatgaa tactgccaat cagatgaaaa tggcgattgc cggggagggt
    31921 gatatcgatg gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac
    31981 ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg
    32041 gatggcgcaa atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg
    32101 catcgctatt cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga
    32161 cgacacccgg agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg
    32221 agttagcaga gttgctgcat gttcatcgta atcgcgtcag tgcactgatc aataacaatc
    32281 gtaaactcac cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt
    32341 ttaggctaaa cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc
    32401 aggaagaatt gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg
    32461 caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttca
    32521 cgccgcatcc ggcatccgag caccattgcc tgatgcgacg cttacgcgtc ttatcaggcc
    32581 tacaaacctg tgcctgaacc gtaggccgga taaggcgttt acgccgcatc cggcagtcat
    32641 gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg caatgacgta cttattcctt
    32701 cggtttaaac cgcagcaacc ggttggcgtt acttactacg gtaattgacg agagcgccat
    32761 tgccgctccg gcaactaccg ggttaagcag tgttccagta aacggccaca aaataccggc
    32821 ggcgaccgga ataccgatgc tgttgtagat aaaggcaccg agcaggttct gcttcatgtt
    32881 gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg cccatcaggc tatggcgcat
    32941 cagggtaatc gccgcggttt caatggcaac atcactgccg ccacccatcg caatgccgac
    33001 atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg ccacctgacg
    33061 tccttcactt tgcagacgtt tgatcgcttc ggctttaccg tccggcagca ctccggcaat
    33121 cacttcatca attccagctt ctttggcgat cgcattggcg gtggttgggt tatccccggt
    33181 caacatcacc agacgatatc ccgctttatg caggcgttgc agcgccgcca cgctatcact
    33241 acgcaacgga tcgcgtacag ccagcagggc tactgctttc ccgtcaatcg ccagcagcac
    33301 aggcgttgct ccttgcgatg cctgagctga aatatcggct tcaatcgctt tagtatcgac
    33361 ttgctgatca tttaacaacg cctgattacc cagcaataac gcatgacctt ctgcttcacc
    33421 gctcacgccc agcccgcgca atgtgcggaa accgttgacc tgcggtagct gcatatcgcc
    33481 tgctttatca aggatcgctc gtgccaatgg atggctggaa ccttgctcca gcgccgccgc
    33541 cagacgcagc gcctgcgctt cgtcaacatc agcaaatgtt ttcactgcga caacctgcgg
    33601 cttcccttcg gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg tactggcgcg
    33661 ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca gccgcccgcc cgacgccgga
    33721 aataatcgac atcggcgtcg ccagtcccag cgcacacggg caggcgataa tcagcaccgt
    33781 agtggcaatc accagggtat agacaatctg cggtgccgga ccaaagaaat accagattgc
    33841 cgcactgaca agcgcaataa ccactactac cggcacaaat atggctgaga ttttatccgc
    33901 cagctgaccg atttctggct tgctgctctg ggcctggcgc accatgcgaa tgatccttga
    33961 tagcgtggta tggctgccaa ccgcactggc acgaaacagc acactgccgt cctgtaccac
    34021 tgtcccggca tggacgctat cgccttcgcc tttttgctgc gggattggtt cgcccgtcag
    34081 catcgcttca tccagccatg cttcgccctg ggtaatctcg ccatctaccg gcacgcgatc
    34141 gccggtcgtc aggcgcagca acatacctgg ctgcacttct gccagaggca cgcttttttc
    34201 accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa tcgagtaatt tctccagtgc
    34261 cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg ccgagattga tcagaccgat
    34321 aatcatcgca ctggcttcat agtaaagatg tcgcgcttcc atcgggaacc actgcggcca
    34381 caggttgacg ctcatcgaat agagccacgc cacgcccgtt cccagcgcca ccagcgtatc
    34441 catcgtcgct gcaccgttca gcaggctttt ccatgcactg cggtaaaaat ggccgctggc
    34501 gaaaaccatc accgccaggg ttatcaggcc gataaccaac cacaggctgc ggttgtcggc
    34561 ggtgactatc atgttatcgc cgatcatccc ccagaccatc accgggatac ccaccgccag
    34621 tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg acggcggttt cttgctggcg
    34681 ttcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt tttccaccgc
    34741 ctgcactaaa tcttgtgggg aggcactgcc catcaccagc gcagtacgct ccgccaggtt
    34801 tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc gggtgacaca
    34861 gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat cggcggtcgc
    34921 tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac gggattgatg actccgccag
    34981 cggtttagcc tttgggtggc ttacagatgc gtcataaccc gcttgtttga tggtttcgat
    35041 cagctgttct gcactggcag tcccggtaac gtgcgcttca gtgatagaca catccgcttg
    35101 ttccacatcc gggcgctgtt caagactttc tttcacgcgt ttaacgcagt gaccgcagga
    35161 caggccgtcc aggttcaggt cgatagtttg tgacataaaa cactccttta agacagtttt
    35221 gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc
    35281 aggcgggtaa aagtccgtaa agattaaaaa atcggctcga tttgcatcag gattagacat
    35341 ttatttcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag gatgcctgaa
    35401 aatcggcact ggggtgagga attacctccc gcatctataa aaaggagtta acaaaagatg
    35461 ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt tcactcactt
    35521 aacggcggac aaaatgccga ttacattccc tttctggcga atgtaccagg tcaactggcg
    35581 gcagtggcta tcgtgacctg cgatggcaac gtctatagtg cgggtgacag tgattaccgc
    35641 tttgcactgg aatccatctc taaagtctgt acgttagccc ttgcgttaga agatgtcggc
    35701 ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt taactcagtt
    35761 atcgccttag agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt
    35821 gccaccacca gcctgattaa cgctgaaaac gccgaacaac gctggcagcg aattttacat
    35881 atccaacagc aactggctgg tgagcaggta gcgctctctg acgaagtcaa ccagtcggaa
    35941 caaacaacca acttccataa tcgggccatt gcctggctgc tgtactccgc cggatatctc
    36001 tattgtgatg caatggaagc ctgtgacgtg tacacccgtc agtgctctac gctcatcaat
    36061 actgttgaac tggcaacgct tggcgcgacg ctggcggcgg gtggtgtgaa tccgttgacg
    36121 cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat gatgatggaa
    36181 gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg caaaagcggt
    36241 gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc gttctcacca
    36301 ccgctggacg aagaaggcaa tagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag
    36361 caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt aataacggta
    36421 acaaacccct cggtctatgg aacgtcgttt ccatcggtat tggggcaatg gtgggggcgg
    36481 ggatcttcgc gctgctgggg caggctgcgt tgctaatgga agcctcgacc tggggcgcct
    36541 ttgcttttgg cggtattgtg gctatgtttt ccggttatgc ctatgcgcgt ctgggggcga
    36601 gctatcccag caatggcggt attatcgact tttttcgtcg cggattaggc aacggcgtct
    36661 tttcgctagc gctctcgttg ctatacctgt tgacgctggc ggtgagcatc gccatggttg
    36721 cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc
    36781 ttattttgct ctacgcgttg gggatcattg cggtgatgac gcttttcaac tccttaagca
    36841 accatgcggt agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat
    36901 tgctgattat tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc
    36961 ccagctccgg tgcgttcttc tcctgtattg ggataacctt ccttgcctat gcgggctttg
    37021 gcatgatggc gaacgcggcg gataaagtga aagatccgca ggtcattatg ccacgggcgt
    37081 ttctggtggc gattggcgtt accacgttgc tttatatctc gctggcgctg gttttgctta
    37141 gcgatgtatc ggcattagag ttagaaaaat atgctgatac cgccgtagcg caggctgctt
    37201 ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg
    37261 cttcaggcat taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg
    37321 aacgcgaact gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaata
    37381 tcatcgtcgt ggtgttgata atgctgatga cggcggcgct aaatttagac tcactcgcca
    37441 gcgttgccag cgccaccttt ttgatctgct atctggcggt gtttgtggtg gcgatccgcc
    37501 tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt
    37561 tggtgatcgt tggctttatc tacagcctgt ggtcccaggg cagccgtgcg ttgatatgga
    37621 ttattggtgc aatcttactc agccttattg tggcaatggt catgaagcgt aataaaaccg
    37681 tataacacct ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca
    37741 ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt
    37801 gcgcgaacga tgaacatcag cgatgtagca aaaattaccg gcctgaccag caaagccatt
    37861 cgcttctatg aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc
    37921 acctacacgc agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc
    37981 tttaacctgg aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc
    38041 gccgacgtca aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag
    38101 ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc
    38161 gccgactgcc cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta
    38221 agacgagacg gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt
    38281 acctgcaccg agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg
    38341 accgcgcccg ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc
    38401 gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt
    38461 ccaccacagc caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca
    38521 acccagcggc accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa
    38581 caaataacca tttccgccca gcatctcggc tgccagcagc aacccgccga gactgagcca
    38641 gaaaatatgt ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg
    38701 tctttcacca gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc
    38761 atcatcacta ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttt
    38821 tgcgctacga agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt
    38881 ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc
    38941 gactgacgtt cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg
    39001 atttccgcct gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct
    39061 ttcatctgcg cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca
    39121 atacgggtga ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt
    39181 gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta
    39241 cggatgttag tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataggca
    39301 gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta
    39361 tctttcgaga taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg
    39421 cgaccaatgc gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta
    39481 tagcgaccaa aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg
    39541 ccgacaatga ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc
    39601 tcctgttgta ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat
    39661 tactgaaaga gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag
    39721 aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat
    39781 ctggcgggtg atacgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt
    39841 aagttaatta ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca
    39901 ttgatcagcc caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaaa
    39961 ccagaaatct accgccagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat
    40021 acggtatacg ataatctgat ctttccctgg cagatccgtg accagcagcc tgacccggcc
    40081 atttttctcg attttctcga acgcttcgct ttgccggaca gcattttgac gaagaatatc
    40141 gccgagctat ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg
    40201 ccgaaggttt tattgctgga tgaaataacc agtgcgctgg atgaaagtaa taaacataac
    40261 gtcaatgaga tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca
    40321 cacgataaag acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga
    40381 gaaatgcagg aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc
    40441 attaatgctg gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa
    40501 agatattcta tggagcgtcg ggcgagcgat aattcagctg attattgtcg gctatgtgct
    40561 gaagtatatt ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg
    40621 ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc
    40681 atcgtttatt gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg
    40741 ggcgattgaa tttatcccga tgcaggtgat ccctgtcgcc gggatgattg ccggtaacgc
    40801 catggtagcg gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca
    40861 acagattcag gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat
    40921 tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt
    40981 agtgagttta ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc
    41041 gattaaatat cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat
    41101 tattgcctgc tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac
    41161 tcaattgaag aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt
    41221 catgtgccga acaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga
    41281 cgccagtgca tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg
    41341 accgtctgcg gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc
    41401 attgcgccca acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg
    41461 attctccgcc acctgctgtt gcaactgttg aatttccggc ttatcagccg cctgcttcag
    41521 cagttcgatt tgcgctacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt
    41581 tttcagcacc gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag
    41641 caggccgatc tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc
    41701 atcggtgtaa ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc
    41761 gcgcggcagc actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa
    41821 gccatctacc ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa
    41881 ctgggcggca atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt
    41941 gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc
    42001 agaccaaaaa tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt
    42061 agattcgtta atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat
    42121 tttctttgac atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat
    42181 cacgcgccca ggcagcaggc gctcgagcac cattactgcc caggtcacca gcgttaccgg
    42241 atagcgcatc ttcggcttct cgctaataaa agcatggcgc actttgtcga ccaccgcttc
    42301 cggtcccaac gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt
    42361 ctggttgacg ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt
    42421 aattccgctg tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt
    42481 actggccgcg taagcgccac gacccggcgt ggagattaat cccatcaccg atgatgtcat
    42541 cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg
    42601 tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag
    42661 ggggccatac atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag
    42721 ggcgatcacc tcgtctgctg cgcgattaac actttctggt gaatccagat cgatcaacac
    42781 gccggtaaat cccatgttgt tcatacgctc aacatcattc ggttttcggc aacctgccag
    42841 cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca
    42901 tccggtaatt aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg
    42961 agtcatgatt tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg
    43021 cgtcgcggtt gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt
    43081 ccataaaaaa gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc
    43141 taaaggcttc attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg
    43201 gttcagcgtt ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct
    43261 gtggctgaaa accacgcaaa ccatcattgc gcccagttca accagcaccc aacgcggctg
    43321 atgctgtttg agcagagccg gaaggcgcgc cagcccttgt tgcgaggtgt cgccgctgat
    43381 gctggcatta actaccgacg ttttactctg ccacttatca ttcaacaagg caggccaggc
    43441 cgcgctggca gacattcgat acccggcgct caggctatca cccagaatca ataacgtgtc
    43501 cgctgcggcg gcacggaagg ttaacaggac caggaacagg aagggcaaat gccagcggaa
    43561 aacattgttg aagttcatca tcttaagaag tccgtcggtc agggggagca tgaactctcc
    43621 atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgag
    43681 tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg ggcttgatga cggcagcagt
    43741 ggcgaagtga gtctggtggg acaaccgcta cataatatgg acgaagaagc gcgggcaaag
    43801 ttgcgcgcga agcacgtcgg ctttgttttt cagtcattta tgttaattcc tacccttaac
    43861 gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt
    43921 aacggggcga aagcgttgct cgaacagtta gggctgggta aacgtctgga tcatcttccg
    43981 gcacagcttt ccggcggtga acagcaacga gtggcgctgg cacgagcctt taatggtcga
    44041 cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg accgccagac gggcgataaa
    44101 attgccgacc tgctgttttc cctcaaccgt gaacatggca ccacgttgat tatggtgacc
    44161 cacgacctgc aactggcggc acgctgcgac cgctgcttac ggctggtgaa cgggcagttg
    44221 caggaggaag catgattgca cgttggttct ggcgcgaatg gcgttcgccg tcgctattaa
    44281 ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg
    44341 atcgcatgga gaagggctta agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt
    44401 tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga agcgcaaaag cgcggcctga
    44461 aagtcggcaa gcagctgact ttcgccacaa tgacctttgc aggcgacaca ccgcagctgg
    44521 cgaacgtcaa agcggtggat gatatctacc cgatgtatgg cggtctgcaa actaatcccc
    44581 ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc acgcctgatg gcactgctta
    44641 acctgaaaac gggcgacacc attgacgtgg gcgatgccac cttgcggatt gccggagaag
    44701 tgattcagga accggattcc ggttttaacc ccttccagat ggctccgcgt ctgatgatga
    44761 atctggcgga tgtcgataaa accggagccg tgcagccggg gagtcgggtc acctggcgtt
    44821 ataaattcgg cggcaacgag aaccagctcg acggctatga gaaatggttg ttacctcagc
    44881 ttaaacccga acaacgctgg tacggtctgg aacaggacga aggcgcgctg gggcgatcga
    44941 tggaacgctc gcaacagttc ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg
    45001 cagcggtggc ggtagcgatg aatcattact gtcgcagtcg ctacgatctg gtggcgatcc
    45061 tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga
    45121 tggtgctgac gctttcagcc gttaccggtg gggccatagg cctgttgttc gaaaacgtgt
    45181 tgatggtgct gctcaagccc gttctgcctg ctgcactacc gccagccagc ctctggccgt
    45241 ggctgtgggc gcttggcacc atgacggtca tctcgctgct ggtggggcta cgaccatacc
    45301 gcttgttgct ggcaacgcag cctttacgcg tattacgtaa tgatgtggtc gcgaacgtct
    45361 ggccgctgaa gttttatctg ccgattgtca gtgtggtggt tgtgctgctg ctcgccggat
    45421 taatgggtgg cagcatgctg ctttgggcgg tgctggcggg cgcggtagta ctggctttgc
    45481 tgtgcggtgt gctgggctgg atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc
    45541 tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc gtggtcaacg ttaagccagc
    45601 tttcggcatt ttcgctctcc tttatgctgc tggcactgct gctggtgctg cgtggcgatc
    45661 tgctcgaccg ctggcagcag cagctaccgc cagaaagccc gaactacttt ttaattaaca
    45721 tcgccacaga acaggtagca ccgctaaaag cgttcctcgc ggaacatcat atagtcccgg
    45781 aatcatttta tccggtagtg cgggcgcggc tgacggcgat taatgataag ccgacagaag
    45841 gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac ctggcaaaat acgcggcccg
    45901 atcataatcc gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag
    45961 aggggctggc gaaacgctta aacgttgccc tcggtgatac cgtgactttt atgggcgata
    46021 cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc
    46081 ctaatttcta ttttattttc cctgaagggg cattagacgg gcaaccgcag agctggctta
    46141 ccagtttccg ctgggagaat ggcaacggca tgttgacgca actcaaccgc cagttcccga
    46201 ccattagcct gttagacatt ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg
    46261 taagtcgggc gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc
    46321 tggcacaggt gcaggtggga atgcgccagc gtcatcagga gctggtggta tggcgcacac
    46381 tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg tgatgttcgc catgcttggg
    46441 tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg
    46501 aaagtgtttg atttcccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc
    46561 ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag
    46621 gcgctgttca ggcagtttgt ggggtgatga caggtgttag attgcctgat gcgctacgct
    46681 tatcaggcct acgaagagcg tgcaatatat tgattttgca agattgtgta ggccggataa
    46741 ggcgtttacg ccgcatctgg catgaacagt gtgcattttg gcaataaata tgaaaccaac
    46801 tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct tcggccttag ctacgtttcc
    46861 tcagttattc aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga
    46921 tgagaacttt atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac
    46981 agcttaccct tacgttgcct ataactatga ttttcttcat gtgctagatg aggactgtct
    47041 ctctccaaat tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta
    47101 cttttccgca ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg
    47161 ccaacatagc tgtttgaata tgtaggcatc ctgacttata attcacttgc tagatgagga
    47221 ctgtttaatt tctgcccgga accaccaagt cttgggcgga aaaaataaaa aaaccgactg
    47281 tcatgtcggt ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attaatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ttcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caactaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg gggggttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agc
//
LOCUS       DAJVQR010000018        52765 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVQR010000018 DAJVQR010000000
VERSION     DAJVQR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..344
                     /locus_tag="OQM52_001118"
     CDS             <1..344
                     /locus_tag="OQM52_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1563054.1"
                     /translation="HIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELEN
                     SEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQI
                     MSRVKIVVMYN"
     gene            complement(382..1281)
                     /gene="nhaR"
                     /locus_tag="OQM52_001119"
     CDS             complement(382..1281)
                     /gene="nhaR"
                     /locus_tag="OQM52_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1563055.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1347..2513)
                     /gene="nhaA"
                     /locus_tag="OQM52_001120"
     CDS             complement(1347..2513)
                     /gene="nhaA"
                     /locus_tag="OQM52_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1563056.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3099..3251
                     /gene="mokC"
                     /locus_tag="OQM52_001121"
     CDS             3099..3251
                     /gene="mokC"
                     /locus_tag="OQM52_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1563057.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3355..4485)
                     /gene="dnaJ"
                     /locus_tag="OQM52_001122"
     CDS             complement(3355..4485)
                     /gene="dnaJ"
                     /locus_tag="OQM52_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1563058.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4574..6490)
                     /gene="dnaK"
                     /locus_tag="OQM52_001123"
     CDS             complement(4574..6490)
                     /gene="dnaK"
                     /locus_tag="OQM52_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1563059.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6867..7271
                     /locus_tag="OQM52_001124"
     CDS             6867..7271
                     /locus_tag="OQM52_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1563060.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7297..8010
                     /gene="msyB"
                     /locus_tag="OQM52_001125"
     CDS             7297..8010
                     /gene="msyB"
                     /locus_tag="OQM52_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1563061.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8159..8725
                     /gene="satP"
                     /locus_tag="OQM52_001126"
     CDS             8159..8725
                     /gene="satP"
                     /locus_tag="OQM52_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1563062.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8760..9347)
                     /gene="mog"
                     /locus_tag="OQM52_001127"
     CDS             complement(8760..9347)
                     /gene="mog"
                     /locus_tag="OQM52_001127"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1563063.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9462..10415)
                     /gene="tal"
                     /locus_tag="OQM52_001128"
     CDS             complement(9462..10415)
                     /gene="tal"
                     /locus_tag="OQM52_001128"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1563064.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10694..12124
                     /locus_tag="OQM52_001129"
                     /pseudo
     CDS             10694..12124
                     /locus_tag="OQM52_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12194..12970
                     /gene="yaaA"
                     /locus_tag="OQM52_001130"
     CDS             12194..12970
                     /gene="yaaA"
                     /locus_tag="OQM52_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1563065.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13123..13419)
                     /locus_tag="OQM52_001131"
     CDS             complement(13123..13419)
                     /locus_tag="OQM52_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1563066.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13633..14919)
                     /gene="thrC"
                     /locus_tag="OQM52_001132"
     CDS             complement(13633..14919)
                     /gene="thrC"
                     /locus_tag="OQM52_001132"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1563067.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14920..15852)
                     /gene="thrB"
                     /locus_tag="OQM52_001133"
     CDS             complement(14920..15852)
                     /gene="thrB"
                     /locus_tag="OQM52_001133"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1563068.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15854..18316)
                     /gene="thrA"
                     /locus_tag="OQM52_001134"
     CDS             complement(15854..18316)
                     /gene="thrA"
                     /locus_tag="OQM52_001134"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1563069.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18342..18461)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18397..18462)
                     /gene="thrL"
                     /locus_tag="OQM52_001135"
     CDS             complement(18397..18462)
                     /gene="thrL"
                     /locus_tag="OQM52_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1563070.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18676..19362)
                     /locus_tag="OQM52_001136"
     CDS             complement(18676..19362)
                     /locus_tag="OQM52_001136"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1563071.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19762..19902)
                     /locus_tag="OQM52_001137"
     CDS             complement(19762..19902)
                     /locus_tag="OQM52_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563072.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19998..20714
                     /gene="arcA"
                     /locus_tag="OQM52_001138"
     CDS             19998..20714
                     /gene="arcA"
                     /locus_tag="OQM52_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1563073.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20773..22125)
                     /gene="creD"
                     /locus_tag="OQM52_001139"
     CDS             complement(20773..22125)
                     /gene="creD"
                     /locus_tag="OQM52_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1563074.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22183..23607)
                     /gene="creC"
                     /locus_tag="OQM52_001140"
     CDS             complement(22183..23607)
                     /gene="creC"
                     /locus_tag="OQM52_001140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1563075.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23607..24296)
                     /gene="creB"
                     /locus_tag="OQM52_001141"
     CDS             complement(23607..24296)
                     /gene="creB"
                     /locus_tag="OQM52_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1563076.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24309..24782)
                     /gene="creA"
                     /locus_tag="OQM52_001142"
     CDS             complement(24309..24782)
                     /gene="creA"
                     /locus_tag="OQM52_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1563077.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24993..25862
                     /gene="robA"
                     /locus_tag="OQM52_001143"
     CDS             24993..25862
                     /gene="robA"
                     /locus_tag="OQM52_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1563078.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25859..26506)
                     /gene="gpmB"
                     /locus_tag="OQM52_001144"
     CDS             complement(25859..26506)
                     /gene="gpmB"
                     /locus_tag="OQM52_001144"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1563079.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26558..27078
                     /gene="yjjX"
                     /locus_tag="OQM52_001145"
                     /pseudo
     CDS             26558..27078
                     /gene="yjjX"
                     /locus_tag="OQM52_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27163..27489)
                     /gene="trpR"
                     /locus_tag="OQM52_001146"
     CDS             complement(27163..27489)
                     /gene="trpR"
                     /locus_tag="OQM52_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1563080.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27579..29516)
                     /gene="sltY"
                     /locus_tag="OQM52_001147"
     CDS             complement(27579..29516)
                     /gene="sltY"
                     /locus_tag="OQM52_001147"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1563081.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29727..31394
                     /gene="ettA"
                     /locus_tag="OQM52_001148"
     CDS             29727..31394
                     /gene="ettA"
                     /locus_tag="OQM52_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1563082.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31501..31668)
                     /locus_tag="OQM52_001149"
     CDS             complement(31501..31668)
                     /locus_tag="OQM52_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563083.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31702..32934)
                     /gene="nadR"
                     /locus_tag="OQM52_001150"
     CDS             complement(31702..32934)
                     /gene="nadR"
                     /locus_tag="OQM52_001150"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1563084.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32955..34337)
                     /gene="radA"
                     /locus_tag="OQM52_001151"
     CDS             complement(32955..34337)
                     /gene="radA"
                     /locus_tag="OQM52_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1563085.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34386..35354)
                     /gene="serB"
                     /locus_tag="OQM52_001152"
     CDS             complement(34386..35354)
                     /gene="serB"
                     /locus_tag="OQM52_001152"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1563086.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35460..36104
                     /locus_tag="OQM52_001153"
     CDS             35460..36104
                     /locus_tag="OQM52_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1563087.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36132..37147
                     /gene="lplA"
                     /locus_tag="OQM52_001154"
                     /pseudo
     CDS             36132..37147
                     /gene="lplA"
                     /locus_tag="OQM52_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37179..37442
                     /locus_tag="OQM52_001155"
     CDS             37179..37442
                     /locus_tag="OQM52_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1563088.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37603..38322)
                     /gene="deoD"
                     /locus_tag="OQM52_001156"
     CDS             complement(37603..38322)
                     /gene="deoD"
                     /locus_tag="OQM52_001156"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1563089.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38379..39602)
                     /gene="deoB"
                     /locus_tag="OQM52_001157"
     CDS             complement(38379..39602)
                     /gene="deoB"
                     /locus_tag="OQM52_001157"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1563090.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39654..40976)
                     /gene="deoA"
                     /locus_tag="OQM52_001158"
     CDS             complement(39654..40976)
                     /gene="deoA"
                     /locus_tag="OQM52_001158"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1563091.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41103..41882)
                     /gene="deoC"
                     /locus_tag="OQM52_001159"
     CDS             complement(41103..41882)
                     /gene="deoC"
                     /locus_tag="OQM52_001159"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1563092.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42140..43681
                     /locus_tag="OQM52_001160"
     CDS             42140..43681
                     /locus_tag="OQM52_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1563093.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43653..44516
                     /locus_tag="OQM52_001161"
     CDS             43653..44516
                     /locus_tag="OQM52_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1563094.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44730..45512)
                     /locus_tag="OQM52_001162"
     CDS             complement(44730..45512)
                     /locus_tag="OQM52_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1563095.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45509..46582)
                     /locus_tag="OQM52_001163"
     CDS             complement(45509..46582)
                     /locus_tag="OQM52_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1563096.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46704..46865)
                     /locus_tag="OQM52_001164"
     CDS             complement(46704..46865)
                     /locus_tag="OQM52_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1563097.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46982..47587)
                     /gene="osmY"
                     /locus_tag="OQM52_001165"
     CDS             complement(46982..47587)
                     /gene="osmY"
                     /locus_tag="OQM52_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS1563098.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47980..49569)
                     /gene="prfC"
                     /locus_tag="OQM52_001166"
     CDS             complement(47980..49569)
                     /gene="prfC"
                     /locus_tag="OQM52_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1563099.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49660..50337)
                     /gene="yjjG"
                     /locus_tag="OQM52_001167"
     CDS             complement(49660..50337)
                     /gene="yjjG"
                     /locus_tag="OQM52_001167"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1563100.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50352..50798)
                     /gene="rimI"
                     /locus_tag="OQM52_001168"
     CDS             complement(50352..50798)
                     /gene="rimI"
                     /locus_tag="OQM52_001168"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1563101.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50767..51180)
                     /gene="holD"
                     /locus_tag="OQM52_001169"
     CDS             complement(50767..51180)
                     /gene="holD"
                     /locus_tag="OQM52_001169"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1563102.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51283..52314
                     /gene="rsmC"
                     /locus_tag="OQM52_001170"
     CDS             51283..52314
                     /gene="rsmC"
                     /locus_tag="OQM52_001170"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1563103.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52591..52677
                     /locus_tag="OQM52_001171"
     tRNA            52591..52677
                     /locus_tag="OQM52_001171"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52625..52627,aa:Leu,seq:cag)
BASE COUNT        12559 a        14271 c        13238 g        12697 t
ORIGIN      
        1 atcatataag atttgactgc atccagggcg agcaaggtga attagttttt gaacccagtg
       61 acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc atattaaaaa
      121 atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc tccaatgatg
      181 gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat ccccatgaag
      241 gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat gtctcaagcg
      301 ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc tcccccacct
      361 taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag aataatccgt
      421 attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa tagcatggta
      481 ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg cataaaagtc
      541 atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa aagctttcat
      601 caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg agttaaacca
      661 gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc gccgttcttc
      721 cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac tcacgccaca
      781 ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg gacagtcaga
      841 aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt gggtggattc
      901 gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta cgctactgac
      961 caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg attctttgcg
     1021 atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg catagcgata
     1081 gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa ataatttgcc
     1141 ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag ttaaataaag
     1201 cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt aatacaagtg
     1261 gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa agggagccgt
     1321 ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac gcgtaaccag
     1381 ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt cgcccagtta
     1441 atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga catagtaaaa
     1501 ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc ctcaggcaga
     1561 tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag cggtttgcca
     1621 atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc cagcgtgacg
     1681 ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag atacgccacc
     1741 cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt ctctttcaaa
     1801 ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga tttcaacacc
     1861 gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac accacacaga
     1921 ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc catcgataag
     1981 tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat aatagccaga
     2041 gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc cagtacacca
     2101 agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt aatcggatcg
     2161 gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat agcggcgata
     2221 actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc acgtttaact
     2281 tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca taacagcatg
     2341 tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag aaagtcgtga
     2401 taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc ggcaatgata
     2461 agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt cactttttat
     2521 ttctctttca ggtgaataga tcgtttttac tattttactc cgcacgaatc atcgttaaaa
     2581 tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt tgcgccatca
     2641 gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag cccagataat
     2701 catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat taatttgttg
     2761 tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt cgtcaataat
     2821 tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta cgccacaaaa
     2881 atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga gagcgatata
     2941 atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca gatagagaaa
     3001 agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac acatgtagag
     3061 tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat aaggcgatga
     3121 ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta acgagaaaag
     3181 acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc acggcttacg
     3241 aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg ctcgcatatc
     3301 ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg gaggttagcg
     3361 ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc gcgggctgtt
     3421 gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct gtttctgctt
     3481 ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac cctgtgcgcc
     3541 accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg tctgggtttc
     3601 gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt cgccacccag
     3661 cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt cacgctcgaa
     3721 aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg cgccatgctc
     3781 gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc ctgccgggat
     3841 tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt tgttgcacgg
     3901 atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta cagcgaagaa
     3961 tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct gcggctgtgt
     4021 acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg gaatgcggat
     4081 ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat agcgtaaatc
     4141 agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc cgaaaacgtc
     4201 accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca tgcctccttg
     4261 ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt cggtcagaac
     4321 ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac cctggttacg
     4381 gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac gctcttccgc
     4441 tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt ttaaattgcc
     4501 cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt ataattaccc
     4561 gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg acaacatcgt
     4621 catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg gcatgttgct
     4681 gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt ttcgcttcga
     4741 tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca gactcgatag
     4801 cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta cgggtgctgt
     4861 gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta cggtcagctt
     4921 cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc agaccagaag
     4981 aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg gaaacgtgca
     5041 ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg cgcggtgccg
     5101 ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca cgtttacgtt
     5161 caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta gagaacacct
     5221 ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc acaccgccca
     5281 tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct tttacgtcac
     5341 cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc gggttaacgt
     5401 ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt ggcatacgag
     5461 tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca gcatcctgca
     5521 gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg ctttccagtt
     5581 tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca gtgatgtatg
     5641 gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt tctgccgctt
     5701 ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga tctttcttga
     5761 attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc aggtgggtat
     5821 caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg atttcgataa
     5881 tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg ttgccagtgc
     5941 ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata cgttttactt
     6001 ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg ttaaagtatg
     6061 ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca gcggttttct
     6121 tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg cctttaactt
     6181 cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg gaaacatcac
     6241 gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca aacagggtgt
     6301 tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt tcaccatcct
     6361 gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc agcacgcgag
     6421 gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg ataccaatta
     6481 ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt gagtctgtaa
     6541 taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac tcactcactg
     6601 cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc aaaaattttt
     6661 tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt gacagcaatt
     6721 ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt aataaaaaga
     6781 aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta ttcatccccc
     6841 tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc gccagcctgg
     6901 cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc ctcggcgtca
     6961 ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt tgtcgcattg
     7021 tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt gcatcggttt
     7081 atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa ataaaagaag
     7141 ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc tgcgtctcaa
     7201 aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg aaaattatcg
     7261 gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta cctgaatgat
     7321 tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt cgcccgattg
     7381 ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg caacgaactg
     7441 tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct gattgccgga
     7501 gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca cggtaaattg
     7561 tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga caaagagatg
     7621 tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac ctggaagaaa
     7681 atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt gaatgagctg
     7741 gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt gtcgcatttg
     7801 ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt acttgggcat
     7861 ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa tggggttaaa
     7921 gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat cgcctgcctg
     7981 cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat tttcattttc
     8041 ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc cctgaaaaca
     8101 ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt gaggaattat
     8161 gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg gcatgaccac
     8221 cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta ttcttgccat
     8281 gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt acaaaaaagg
     8341 caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga cgctggttgc
     8401 gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt tccttggtgt
     8461 ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc tgaaaggcgc
     8521 gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc tggcgatcgg
     8581 taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc tgatctgcgg
     8641 tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg gtcgcaccgt
     8701 tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg gggatttttt
     8761 tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac ttctggtgcc
     8821 gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc aaaaatacca
     8881 tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt aatagacttc
     8941 ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac ctgacgcgaa
     9001 aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc aaagccaggc
     9061 atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg cgccgggcca
     9121 gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc acacaacgtt
     9181 tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa cggcgtggtt
     9241 agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata aacgccgctg
     9301 gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa gatttttccg
     9361 tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga caggcttcag
     9421 aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag atcgccgatc
     9481 attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc cagtttatct
     9541 actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat acgcgccgga
     9601 cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc gctctccgcc
     9661 agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag ttccagaatt
     9721 tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg ctctttgtag
     9781 tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc gtactctttc
     9841 ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga gatcaggaac
     9901 acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt caggttacag
     9961 ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt agaagccagt
    10021 ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat caggcgtttt
    10081 gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc agttgagata
    10141 cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt gtcggtcgcg
    10201 tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac agcatcatca
    10261 atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg gttggttgtg
    10321 gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc cactacggtg
    10381 gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt taaacagctt
    10441 gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca gcgcaaccgc
    10501 cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc gttgatatcg
    10561 cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag tacctcgtag
    10621 cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc gaccggcaat
    10681 aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt ggggatcggt
    10741 aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg gatttgtgca
    10801 gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc cacagccagg
    10861 cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg gtagcggcaa
    10921 cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct tttggatgtg
    10981 ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg cacaacttta
    11041 taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata tggcgcgcgg
    11101 gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg cctacggcat
    11161 aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt cttttgattt
    11221 tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa tcactcgtgg
    11281 tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt
    11341 actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc
    11401 tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt
    11461 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg tgggaatggg
    11521 atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg cagcacaagg
    11581 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
    11641 gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta ttcagcttat
    11701 ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta ccctcgtggt
    11761 tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt
    11821 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
    11881 ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag atatcataat
    11941 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
    12001 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
    12061 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
    12121 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
    12181 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
    12241 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc agttgatcca
    12301 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
    12361 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggcgaatgc
    12421 ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt
    12481 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
    12541 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
    12601 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
    12661 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
    12721 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
    12781 gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg gtctgatgag
    12841 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
    12901 aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta aacgctacga
    12961 gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    13021 agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc gccagcgtcg
    13081 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
    13141 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
    13201 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
    13261 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
    13321 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
    13381 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
    13441 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
    13501 atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct gtcgtttttc
    13561 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
    13621 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa aatcggcggg
    13681 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
    13741 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
    13801 gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc
    13861 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
    13921 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
    13981 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
    14041 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
    14101 ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca gacccgccgt
    14161 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cctcctgcgg
    14221 cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
    14281 cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
    14341 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
    14401 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
    14461 cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg tatcaccgga
    14521 ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca tttgtgccat
    14581 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
    14641 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
    14701 ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
    14761 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
    14821 gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
    14881 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
    14941 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
    15001 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
    15061 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
    15121 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
    15181 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
    15241 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
    15301 cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac
    15361 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
    15421 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
    15481 cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
    15541 gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct tttccagagt
    15601 catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac
    15661 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt tgttgagact
    15721 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgc
    15781 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
    15841 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
    15901 caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
    15961 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
    16021 cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa
    16081 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
    16141 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
    16201 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga
    16261 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
    16321 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
    16381 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac
    16441 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
    16501 catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca
    16561 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
    16621 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
    16681 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg
    16741 caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg
    16801 agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac
    16861 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
    16921 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
    16981 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
    17041 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
    17101 taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta
    17161 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt
    17221 cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
    17281 cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca accatccctt
    17341 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
    17401 ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct tgaggatttc
    17461 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
    17521 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
    17581 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
    17641 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
    17701 cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa cctgccatca
    17761 acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat
    17821 cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg
    17881 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
    17941 cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat
    18001 gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg
    18061 ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
    18121 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
    18181 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
    18241 tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga
    18301 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg
    18361 tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt
    18421 aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg tgtactctgt
    18481 aatttttatc tgtttgtgcg ctatgcctat attggttaaa gtatttagtg acctaagtca
    18541 ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc agacactctt
    18601 tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc tgaaaaatac
    18661 ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca aacggtgcaa
    18721 cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt cgaccagttt
    18781 tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc gtaaagcttg
    18841 cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta atgttgctaa
    18901 ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat ccgccaccat
    18961 cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc cggaatcttc
    19021 gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta acagcggcac
    19081 cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag tggtggcgac
    19141 agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa tattatcaat
    19201 aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg cctgactatc
    19261 gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg ccccaatatt
    19321 ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac tcttcttgac
    19381 caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa tttacaaatt
    19441 cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa actattttct
    19501 gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt acttaggata
    19561 attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca
    19621 actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc
    19681 aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg
    19741 tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt
    19801 tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt
    19861 gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta
    19921 atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg
    19981 caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa
    20041 cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag
    20101 atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata
    20161 tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg
    20221 ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa
    20281 tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac
    20341 gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg
    20401 aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg
    20461 atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa
    20521 acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga
    20581 aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta
    20641 cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc ttctgcggtg
    20701 atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta gcgccgtttt
    20761 tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc
    20821 tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa acatcatgcc
    20881 ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag
    20941 cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg
    21001 ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc
    21061 gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa
    21121 catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag
    21181 cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt
    21241 cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg
    21301 gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa
    21361 ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt tggctggtag
    21421 aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg
    21481 cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctg
    21541 caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg
    21601 ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac
    21661 cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat
    21721 atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc
    21781 actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc
    21841 atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt
    21901 tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac
    21961 gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc
    22021 ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca gcaatactgc
    22081 accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc
    22141 aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg
    22201 aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt
    22261 aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt
    22321 tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc
    22381 actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt
    22441 tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg
    22501 atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat gcaaagtgat
    22561 gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac
    22621 atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt
    22681 ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa aacgagccac
    22741 tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg
    22801 gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag
    22861 cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc
    22921 gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat
    22981 ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccaacaaaat
    23041 ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt
    23101 cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg gcgcggcaac
    23161 atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg
    23221 accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa cggctttatt
    23281 tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat attcattgcg
    23341 cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct gattaaacgc
    23401 ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc
    23461 aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac
    23521 ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc
    23581 cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc catgccgcga
    23641 tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg cagcgtttta
    23701 atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt
    23761 agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac
    23821 tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc
    23881 aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt cacccgacgc
    23941 agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg
    24001 tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac
    24061 agtacaggta acgccggata gagcgccagt aactggcggc acaattcaaa gccactgata
    24121 tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc
    24181 accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc
    24241 agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat
    24301 cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa
    24361 ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa
    24421 aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg
    24481 ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa
    24541 gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat accaccggtt
    24601 ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca
    24661 acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct
    24721 gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc
    24781 atattgttac catttttatc tattacacga gcaattagtt cgtcacggta aataacatat
    24841 tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca tactctacta
    24901 agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca
    24961 ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc
    25021 ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga
    25081 aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta
    25141 ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg
    25201 cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc
    25261 gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga
    25321 gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat
    25381 ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg
    25441 agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca
    25501 acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg agtcaggata
    25561 aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct
    25621 atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg
    25681 aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa
    25741 tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg
    25801 ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt
    25861 aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa
    25921 cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt
    25981 tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac
    26041 atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc
    26101 aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa
    26161 tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca
    26221 gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa
    26281 agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta
    26341 aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg
    26401 cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg
    26461 cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta
    26521 tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac
    26581 caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg
    26641 atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac cctttggcag
    26701 tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga
    26761 ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt tcagttgggt
    26821 ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc cgttacctgc
    26881 ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc gcgttatacc
    26941 ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc
    27001 actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc
    27061 gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat tcggtgcacg
    27121 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa
    27181 cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt tagatccacg
    27241 cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc
    27301 acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt
    27361 cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc
    27421 gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg
    27481 ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc ccggttgtac
    27541 tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc
    27601 gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc atcataagcc
    27661 agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca
    27721 gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta
    27781 taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa
    27841 ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct gctataacca
    27901 ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg
    27961 ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc
    28021 atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa
    28081 gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg
    28141 ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt
    28201 gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag attccagtac
    28261 atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc aacattctgc
    28321 ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc aaccatcggg
    28381 tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg
    28441 cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt tgcttccatc
    28501 ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag cgccattcgc
    28561 acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc gcgccatttc
    28621 gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc gcgcagctcc
    28681 tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca
    28741 ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg acgggtaaaa
    28801 tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa
    28861 atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc
    28921 aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct
    28981 tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg
    29041 ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat
    29101 ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct
    29161 aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg
    29221 cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga
    29281 ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc
    29341 agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc ctgcttgatt
    29401 tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc
    29461 agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc
    29521 gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa
    29581 ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat
    29641 cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa ctcatcatca
    29701 cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg
    29761 gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg
    29821 gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg ctgcgcatta
    29881 tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg
    29941 gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag
    30001 aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg
    30061 ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca
    30121 ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctgc
    30181 gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtgg
    30241 cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc
    30301 acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc gaaggcaccg
    30361 tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac
    30421 tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg gagcagaaag
    30481 atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag
    30541 agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg
    30601 cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc aacgaactgt
    30661 ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat
    30721 cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcgatcg
    30781 tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctccggtc
    30841 aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg gcatcggttg
    30901 atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc
    30961 tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta
    31021 actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt gagcgcggtc
    31081 gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa
    31141 ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg
    31201 gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc
    31261 tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag
    31321 agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc aagtacaagc
    31381 gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc cggcctacaa
    31441 aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt aagcgtcgca
    31501 tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt ctacaacagc
    31561 cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac cttgtttaat
    31621 ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt tatacctcgc
    31681 ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca ccagctccac
    31741 gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt cgatattgtt
    31801 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
    31861 gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa
    31921 acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt actttttgca
    31981 gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg cgtatttcac
    32041 tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg aatactgcaa
    32101 tgcgatttca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt cccacgcact
    32161 ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc
    32221 gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa tatattccca
    32281 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg
    32341 atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
    32401 cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt tgatgccgtt
    32461 gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga aagcatgaat
    32521 acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat ccggcaccgt
    32581 gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc
    32641 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
    32701 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gccggggaaa
    32761 ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc ttttaatttt
    32821 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg
    32881 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
    32941 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
    33001 ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc
    33061 cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg caccgggcgg
    33121 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca
    33181 cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc ggttaccttc
    33241 acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc accgtgacgg
    33301 tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc
    33361 ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt
    33421 ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa
    33481 attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
    33541 tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg ggagtcggca
    33601 tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg gccagccagc
    33661 gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt tttggcgaaa
    33721 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca
    33781 tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg ctcttcttcg
    33841 gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt gtcagtcggc
    33901 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc ggtgacatac
    33961 agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt ggatttcccc
    34021 gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc tcctagtacg
    34081 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag
    34141 agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc
    34201 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca
    34261 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
    34321 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atgacgggca
    34381 ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg
    34441 tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc
    34501 agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc
    34561 gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt gtactgcgcg
    34621 tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
    34681 gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa agtaaagccg
    34741 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag ctgcgttaag
    34801 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    34861 agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    34921 tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc atcaatacat
    34981 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    35041 gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg tgtagcccgt
    35101 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    35161 atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca
    35221 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    35281 ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag
    35341 gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    35401 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    35461 tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg ttctgtcttg
    35521 ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc
    35581 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg
    35641 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt
    35701 taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg
    35761 cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt aaaaaagcag
    35821 gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct
    35881 atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    35941 ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc
    36001 gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca
    36061 gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    36121 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    36181 acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc
    36241 tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg aaagagtgta
    36301 atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc ggtggcggcg
    36361 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
    36421 ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg
    36481 aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc aaagtctcag
    36541 gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg ctactcaatg
    36601 ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    36661 gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggctcacca
    36721 tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg agcacgtgga
    36781 agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc
    36841 ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc atctgctgga
    36901 tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac
    36961 ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg
    37021 actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt
    37081 tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt
    37141 aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg gaaaaccagg
    37201 aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc gccgtgcgta
    37261 tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc
    37321 taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atggcagaaa
    37381 tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat
    37441 gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat gaaaaataat
    37501 agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg agcagtcccc
    37561 cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt tatcgcccag
    37621 cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc
    37681 agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca gggctttcgc
    37741 gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc cgaggatgcc
    37801 gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa
    37861 caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg cgttacgcac
    37921 catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc ggttaacttt
    37981 ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt gcggcagaac
    38041 tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga tcagttcttt
    38101 ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa ttttgcggcc
    38161 tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc
    38221 aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc
    38281 agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat cctttttgtg
    38341 acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc tttgccatat
    38401 tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc gaaggtttca
    38461 cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg aatgtgttca
    38521 cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt gaggatcagg
    38581 atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa cagttccaga
    38641 cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa gtcaacgaag
    38701 ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt ggcgtcaaac
    38761 agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc gtagatgtct
    38821 gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt ctgcagcacg
    38881 gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg gaagttacca
    38941 gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc gccgttggtc
    39001 agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa agtttcttca
    39061 tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt gccggttttc
    39121 atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca gttaccgagg
    39181 taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg gaagctgttt
    39241 tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat ttcccagtga
    39301 ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc gccgataact
    39361 tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg tgctttcgcc
    39421 agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt atcagcttcg
    39481 cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc accaaagcgt
    39541 tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa tgcacgtttc
    39601 atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata ccgttattcg
    39661 ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag tttaattgcc
    39721 gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag gataaccgcc
    39781 agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa gccgacgctg
    39841 taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac tgccatcccc
    39901 agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac tgctttcgtc
    39961 agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg gcctttttgt
    40021 gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac cgcctgcaat
    40081 ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat ctccacgcac
    40141 agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag gaactgcacc
    40201 gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt catgtcggtg
    40261 agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc ttcggcaagg
    40321 gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac gtccatcacc
    40381 agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat cagcgggatg
    40441 gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc cggagccagt
    40501 gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg gaaacggttg
    40561 tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt accgccagta
    40621 tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc gaccatcggc
    40681 cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc aacaatcggg
    40741 ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat cgccatggtc
    40801 agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc gagggcggca
    40861 atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg aatttcttca
    40921 tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag aaacaaggta
    40981 acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc cttatccggc
    41041 ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt aaagcatctt
    41101 acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag caggcttgcc
    41161 agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc accgaacagt
    41221 tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc gcccgccggt
    41281 ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat gatgcgagcg
    41341 ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa gtccgcaccc
    41401 gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag ttcgccggtt
    41461 tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca ggctttcacc
    41521 aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa caccacgtca
    41581 acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat ttcgatgtcg
    41641 tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt gccctgctct
    41701 ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc ggcggtattg
    41761 ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt gtcgtcgtca
    41821 ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt cagatcagtc
    41881 atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt agtattctaa
    41941 catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac ttacaagttt
    42001 gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc gtcagtggtg
    42061 gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg catcaccttc
    42121 ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac aacgttgcca
    42181 gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc tggcactgga
    42241 agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg cgctggatga
    42301 aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct atgtcttacc
    42361 cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag gcgcgaaaga
    42421 tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt cggtcacatc
    42481 gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta gaattctaac
    42541 acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca gaaccctgcc
    42601 agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc gcgcgatctt
    42661 acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg aaatcacccc
    42721 tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac cgcacatcgc
    42781 caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg tgagctgtta
    42841 caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc tgaaagccat
    42901 tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc actactgcca
    42961 gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat cacacttctt
    43021 tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg tgccaatgtt
    43081 tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag ggattgccgc
    43141 gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg cgcaactggc
    43201 ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca tgttacacgc
    43261 acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc cgtatggcga
    43321 tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt attattattc
    43381 cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc aggcactggt
    43441 acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca atgtcagcgg
    43501 taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag aaaaatatcg
    43561 cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac gcaacactcg
    43621 tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct ttagtcagta
    43681 agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg tttttgcagg
    43741 gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt tgcaatgact
    43801 gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac ggcaaagtgg
    43861 tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt ccgcaacatg
    43921 ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg cgcaaagcgg
    43981 tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa ctgccgtttg
    44041 tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc acctgtctgg
    44101 tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg gtgtgtgacg
    44161 gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta accggacgcg
    44221 ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag ctggcggaac
    44281 tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa gaactggcgg
    44341 cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat gcccacggcg
    44401 tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag ccgttggctg
    44461 atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat ttgtgacatg
    44521 ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg tcaagcgtcg
    44581 catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg gcctacggtt
    44641 cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca ttatgcgccg
    44701 actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac cgcatacgtg
    44761 ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc gcaaagcacg
    44821 gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc gttgagcggc
    44881 atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc gatgacatcg
    44941 cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt gtagcccagc
    45001 tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac accagcacgc
    45061 ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg tgaatgcagg
    45121 atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa ccactgctgc
    45181 ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac cgccaccacc
    45241 ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc gctatgtttt
    45301 tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt ttccgctaac
    45361 gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt gcctacgccc
    45421 gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg gaaatcaaaa
    45481 tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta aatgtcgggt
    45541 cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg actggcgcag
    45601 gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta agcggcgctt
    45661 tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa cgcccaagtt
    45721 tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt aatggcttcg
    45781 gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat tgctgaatgt
    45841 cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg ctactgtcac
    45901 ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt gacggcacag
    45961 tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc ggaatcgcat
    46021 cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta cgataaaagc
    46081 caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt ggtaaaaagt
    46141 aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg ccgctgtcaa
    46201 acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc tccaccagcc
    46261 agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa aattcgcgtt
    46321 ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag ataaacgccg
    46381 agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta aactgcgcgc
    46441 gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg ccttcgcaca
    46501 ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata ttaccaagcg
    46561 ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag aaagacaaag
    46621 taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg gctttggata
    46681 aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa actcactagg
    46741 aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt accggccaga
    46801 ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat gccccaacga
    46861 aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg tgaaaatcac
    46921 tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc gggaggacga
    46981 attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct ttggcgatac
    47041 tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga accacgccat
    47101 cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc agtttggctt
    47161 tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct tctttagcgt
    47221 cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc gccactttca
    47281 ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg actttttgat
    47341 cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc gccgccttca
    47401 ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata gagctatcga
    47461 ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg
    47521 tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc agtcttgtca
    47581 tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac acgaggttgc
    47641 attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca tcacaaaagc
    47701 gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat cggtagaatg
    47761 acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac gccatcagaa
    47821 atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat atcagcgaaa
    47881 acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag gaagggaaat
    47941 tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc gggtctggtg
    48001 gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat
    48061 gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac gcttgaactc
    48121 ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt tgactgactc
    48181 atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat caaactgcag
    48241 cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca cctgcaccgc
    48301 gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca gcggatcttt
    48361 caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga acttcatcat
    48421 ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca ggccgaggat
    48481 atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg tcagcgcgtc
    48541 ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt tttcatattt
    48601 accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca tgttggcctg
    48661 aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac gggtatcggt
    48721 ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat gatcaacgcc
    48781 gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc
    48841 tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca gctgctgtgc
    48901 cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa caatgcggac
    48961 ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt tataaaggtg
    49021 gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg cgcagccaat
    49081 tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat cacggtcaag
    49141 tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg
    49201 ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt
    49261 cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac
    49321 caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc cacgctgctt
    49381 ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt ttactgtacc
    49441 ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag ttttaccggc
    49501 gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca aataaggaga
    49561 caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa
    49621 aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac acaggagctg
    49681 ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg
    49741 attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga gaatatcgga
    49801 ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg cctgttccag
    49861 cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg aaatcaccag
    49921 caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca aggcgctaaa
    49981 accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca gagaaaccgc
    50041 gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt taagcaagcc
    50101 tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa gctgtaatga
    50161 agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct gatagtcctg
    50221 aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca ggccggtgaa
    50281 tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc acttcattat
    50341 tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc gcgaccgtcc
    50401 gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg
    50461 gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac gccgcgtttt
    50521 tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg ctgatagtcg
    50581 ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt aatcgcaaac
    50641 gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc ctggttgctg
    50701 gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg gtaagccgct
    50761 ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt gttgccataa
    50821 cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc
    50881 gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc gactgccttg
    50941 cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc tgacggttaa
    51001 tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat
    51061 caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg cgccagggcg
    51121 acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat
    51181 agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac
    51241 atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc atttaccccg
    51301 gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat tctgtttgcc
    51361 ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg tgctcatacc
    51421 cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc ccgttttagt
    51481 ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta ctggccgaag
    51541 aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc agtggggaca
    51601 gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca gatgctggca
    51661 gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta ttttggtcgt
    51721 ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag cgtcgatggc
    51781 ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt cggtagccag
    51841 ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt cggctgtggc
    51901 gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct caccttgtgc
    51961 gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa cggtgttgaa
    52021 ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga tatgatcatc
    52081 tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca aacgctgatt
    52141 cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc gaacgccttc
    52201 ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat cgcgcaaacc
    52261 gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg ttgagtatct
    52321 gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg acctacaaaa
    52381 cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc tggcatctgc
    52441 ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc cagcaattac
    52501 acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg cgcctccgtg
    52561 gtagcaattc tttttaagaa ttgatggtat gcgaaggtgg cggaattggt agacgcgcta
    52621 gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct cgcaccaaaa
    52681 atcacgttga tattgctcgc actgggcgaa ggtggcggaa ttggtagacg cgctagcttc
    52741 aggtgttagt gttcttacgg acgtg
//
LOCUS       DAJVQR010000019        49821 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVQR010000019 DAJVQR010000000
VERSION     DAJVQR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..1215
                     /locus_tag="OQM52_001172"
     CDS             <1..1215
                     /locus_tag="OQM52_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1563104.1"
                     /translation="PEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHPVI
                     TGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMIIPE
                     IAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALL
                     ACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNT
                     KGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKV
                     PFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLN
                     LPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLL
                     AQFGVDIGEAAQ"
     gene            1212..2177
                     /gene="yggF"
                     /locus_tag="OQM52_001173"
     CDS             1212..2177
                     /gene="yggF"
                     /locus_tag="OQM52_001173"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1563105.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2199..2708
                     /gene="fumE"
                     /locus_tag="OQM52_001174"
     CDS             2199..2708
                     /gene="fumE"
                     /locus_tag="OQM52_001174"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1563106.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2705..3418
                     /locus_tag="OQM52_001175"
     CDS             2705..3418
                     /locus_tag="OQM52_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1563107.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3390..4067)
                     /locus_tag="OQM52_001176"
     CDS             complement(3390..4067)
                     /locus_tag="OQM52_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1563108.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4061..4738)
                     /locus_tag="OQM52_001177"
     CDS             complement(4061..4738)
                     /locus_tag="OQM52_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1563109.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4726..5109)
                     /locus_tag="OQM52_001178"
     CDS             complement(4726..5109)
                     /locus_tag="OQM52_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1563110.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5125..5433)
                     /locus_tag="OQM52_001179"
     CDS             complement(5125..5433)
                     /locus_tag="OQM52_001179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563111.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5434..6012)
                     /locus_tag="OQM52_001180"
     CDS             complement(5434..6012)
                     /locus_tag="OQM52_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1563112.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6035..6466)
                     /locus_tag="OQM52_001181"
                     /pseudo
     CDS             complement(6035..6466)
                     /locus_tag="OQM52_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6838..7857
                     /gene="epd"
                     /locus_tag="OQM52_001182"
     CDS             6838..7857
                     /gene="epd"
                     /locus_tag="OQM52_001182"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1563113.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7907..9070
                     /gene="pgk"
                     /locus_tag="OQM52_001183"
     CDS             7907..9070
                     /gene="pgk"
                     /locus_tag="OQM52_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1563114.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9285..10364
                     /gene="fbaA"
                     /locus_tag="OQM52_001184"
     CDS             9285..10364
                     /gene="fbaA"
                     /locus_tag="OQM52_001184"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1563115.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10555..11415
                     /gene="mscS"
                     /locus_tag="OQM52_001185"
     CDS             10555..11415
                     /gene="mscS"
                     /locus_tag="OQM52_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1563116.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11554..12189
                     /gene="argO"
                     /locus_tag="OQM52_001186"
     CDS             11554..12189
                     /gene="argO"
                     /locus_tag="OQM52_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1563117.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12282..13022
                     /locus_tag="OQM52_001187"
     CDS             12282..13022
                     /locus_tag="OQM52_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1563118.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13189..14139
                     /locus_tag="OQM52_001188"
     CDS             13189..14139
                     /locus_tag="OQM52_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1563119.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14081..15559)
                     /gene="scpC"
                     /locus_tag="OQM52_001189"
                     /pseudo
     CDS             complement(14081..15559)
                     /gene="scpC"
                     /locus_tag="OQM52_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15583..16368)
                     /gene="scpB"
                     /locus_tag="OQM52_001190"
     CDS             complement(15583..16368)
                     /gene="scpB"
                     /locus_tag="OQM52_001190"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1563120.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16379..17374)
                     /gene="meaB"
                     /locus_tag="OQM52_001191"
     CDS             complement(16379..17374)
                     /gene="meaB"
                     /locus_tag="OQM52_001191"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1563121.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17367..19511)
                     /gene="scpA"
                     /locus_tag="OQM52_001192"
     CDS             complement(17367..19511)
                     /gene="scpA"
                     /locus_tag="OQM52_001192"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS1563122.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19715..20608)
                     /gene="argP"
                     /locus_tag="OQM52_001193"
     CDS             complement(19715..20608)
                     /gene="argP"
                     /locus_tag="OQM52_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1563123.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20991..21650
                     /gene="rpiA"
                     /locus_tag="OQM52_001194"
     CDS             20991..21650
                     /gene="rpiA"
                     /locus_tag="OQM52_001194"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1563124.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21906..23138
                     /gene="serA"
                     /locus_tag="OQM52_001195"
     CDS             21906..23138
                     /gene="serA"
                     /locus_tag="OQM52_001195"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1563125.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23367..23426
                     /gene="ibsC"
                     /locus_tag="OQM52_001196"
     CDS             23367..23426
                     /gene="ibsC"
                     /locus_tag="OQM52_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1563126.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23759..23818
                     /locus_tag="OQM52_001197"
     CDS             23759..23818
                     /locus_tag="OQM52_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1563127.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23919..24521)
                     /locus_tag="OQM52_001198"
     CDS             complement(23919..24521)
                     /locus_tag="OQM52_001198"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1563128.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24542..24725)
                     /gene="ssrS"
                     /locus_tag="OQM52_001199"
     ncRNA           complement(24542..24725)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQM52_001199"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24767..25096)
                     /gene="zapA"
                     /locus_tag="OQM52_001200"
     CDS             complement(24767..25096)
                     /gene="zapA"
                     /locus_tag="OQM52_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1563129.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25264..25842
                     /gene="ygfB"
                     /locus_tag="OQM52_001201"
     CDS             25264..25842
                     /gene="ygfB"
                     /locus_tag="OQM52_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1563130.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25868..27193
                     /gene="pepP"
                     /locus_tag="OQM52_001202"
     CDS             25868..27193
                     /gene="pepP"
                     /locus_tag="OQM52_001202"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1563131.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27190..28368
                     /gene="ubiH"
                     /locus_tag="OQM52_001203"
                     /gene_synonym="visB"
     CDS             27190..28368
                     /gene="ubiH"
                     /locus_tag="OQM52_001203"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1563132.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATVPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28391..29593
                     /gene="ubiI"
                     /locus_tag="OQM52_001204"
                     /gene_synonym="visC"
     CDS             28391..29593
                     /gene="ubiI"
                     /locus_tag="OQM52_001204"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1563133.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29667..29750)
                     /locus_tag="OQM52_001205"
     CDS             complement(29667..29750)
                     /locus_tag="OQM52_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563134.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30041..31135
                     /gene="gcvT"
                     /locus_tag="OQM52_001206"
     CDS             30041..31135
                     /gene="gcvT"
                     /locus_tag="OQM52_001206"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1563135.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31159..31548
                     /gene="gcvH"
                     /locus_tag="OQM52_001207"
     CDS             31159..31548
                     /gene="gcvH"
                     /locus_tag="OQM52_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1563136.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31667..34540
                     /gene="gcvP"
                     /locus_tag="OQM52_001208"
     CDS             31667..34540
                     /gene="gcvP"
                     /locus_tag="OQM52_001208"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1563137.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34807..35550
                     /locus_tag="OQM52_001209"
     CDS             34807..35550
                     /locus_tag="OQM52_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1563138.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35607..37040)
                     /gene="bglA"
                     /locus_tag="OQM52_001210"
     CDS             complement(35607..37040)
                     /gene="bglA"
                     /locus_tag="OQM52_001210"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1563139.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37085..37395
                     /locus_tag="OQM52_001211"
                     /pseudo
     CDS             37085..37395
                     /locus_tag="OQM52_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37559..38218
                     /locus_tag="OQM52_001212"
     CDS             37559..38218
                     /locus_tag="OQM52_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1563140.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38414..39394)
                     /gene="ygfZ"
                     /locus_tag="OQM52_001213"
     CDS             complement(38414..39394)
                     /gene="ygfZ"
                     /locus_tag="OQM52_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1563141.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39637..39903
                     /gene="sdhE"
                     /locus_tag="OQM52_001214"
     CDS             39637..39903
                     /gene="sdhE"
                     /locus_tag="OQM52_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1563142.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39884..40291
                     /locus_tag="OQM52_001215"
     CDS             39884..40291
                     /locus_tag="OQM52_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCS1563143.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40331..40852)
                     /gene="fldB"
                     /locus_tag="OQM52_001216"
     CDS             complement(40331..40852)
                     /gene="fldB"
                     /locus_tag="OQM52_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1563144.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40964..41860
                     /gene="xerD"
                     /locus_tag="OQM52_001217"
     CDS             40964..41860
                     /gene="xerD"
                     /locus_tag="OQM52_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1563145.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41885..42595
                     /gene="dsbC"
                     /locus_tag="OQM52_001218"
     CDS             41885..42595
                     /gene="dsbC"
                     /locus_tag="OQM52_001218"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1563146.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42601..44334
                     /gene="recJ"
                     /locus_tag="OQM52_001219"
     CDS             42601..44334
                     /gene="recJ"
                     /locus_tag="OQM52_001219"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1563147.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44425..45523
                     /gene="prfB"
                     /locus_tag="OQM52_001220"
     CDS             join(44425..44499,44501..45523)
                     /gene="prfB"
                     /locus_tag="OQM52_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1563148.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45533..47050
                     /gene="lysS"
                     /locus_tag="OQM52_001221"
     CDS             45533..47050
                     /gene="lysS"
                     /locus_tag="OQM52_001221"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1563149.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47093..47641)
                     /gene="idi"
                     /locus_tag="OQM52_001222"
     CDS             complement(47093..47641)
                     /gene="idi"
                     /locus_tag="OQM52_001222"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1563150.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47696..47767
                     /locus_tag="OQM52_001223"
     CDS             47696..47767
                     /locus_tag="OQM52_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563151.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47764..47889)
                     /locus_tag="OQM52_001224"
     CDS             complement(47764..47889)
                     /locus_tag="OQM52_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563152.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47891..49339)
                     /gene="uacT"
                     /locus_tag="OQM52_001225"
     CDS             complement(47891..49339)
                     /gene="uacT"
                     /locus_tag="OQM52_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1563153.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12049 a        12449 c        13404 g        11919 t
ORIGIN      
        1 ccagaaatta ccgataatga actgttagtg agtgtaattt ctgacagcgt ctgtttatcg
       61 acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga tttagaaaat
      121 catccggtca ttaccgggca tgaatgtgcc ggggttattg tcgaagtggg taaaaatctc
      181 actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg gttaccaagc
      241 ggatattcag cgggctacag ctacgaatat tttggcggca atgccactta tatgattatt
      301 cccgaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta ttttgctgcg
      361 gcgtcgctgg cagagcctat gtgctgcatt attggtgctt atcatgccaa ttatcacacc
      421 acgcaatatg tttatgagca tcgcatgggc gtcaaacctg gcggcaatat tgcactgctg
      481 gcgtgtgcgg gtccgatggg cattggcgct atcgattacg ccattaacgg tggcatacaa
      541 ccgtcacggg tggtggtggt cgatatcgac gacaaacgcc tggcgcaggt gcagaagctg
      601 ctgccggtgg atctggcggc cagtaaaggc attgagctgg tgtatgtgaa taccaaaggg
      661 atgagcgatc ctatccagat gctgcgggcg ctgacaggag atgtcgggtt cgatgacatt
      721 tttgtttatg cggcggtgcc tgctgtcgtt gagatggctg acgaattact ggcggaagat
      781 ggctgtctga acttctttgc cgggctgacg gataaaaact tcaaagtgcc gtttaatttc
      841 tacaacgtcc attacaacag cacgcacgta gtcggtacat ccggcggttc aacggacgac
      901 atgaaagagg cgattgccct tagcgccaca ggacagttac agccgtcgtt tatggtgacc
      961 catattggtg gcctggatgc ggtgccagaa accgtgctca atctgccgga tatccctggc
     1021 ggtaaaaaac tcatttataa cggcgtgacc atgccgctca ctgccattgc cgattttgcc
     1081 gaaaaaggca aaaccgatcc gctgtttaaa gagttggcgc ggctggttga ggaaacgcac
     1141 ggcatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt tgatatcggg
     1201 gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa caggcagcgt
     1261 tggctgcctg gccgcaaacc ggatgtggcg acaaaaataa aattgatggc ctggcggtca
     1321 ccgcgatgcg ccaggcatta aacgacgtcg cttttcgtgg acgagtggtt atcggcgaag
     1381 gagaaattga ccatgcgccg atgctgtgga ttggcgaaga ggtgggcaaa ggcgatgggc
     1441 cagaagtcga tatcgcggtt gatcccattg aaggcacgcg gatggtggcg atggggcaga
     1501 gcaatgcgct ggcggtcatg gctttcgctc ctcgagatag cctgttacat gcgcccgata
     1561 tgtatatgaa aaagctggtt gttaatcgac tggctgccgg ggcgattgat ctgtctttgc
     1621 ctctggcgga caacttgcgt aacgtggcgc gggcgttagg taaaccgctg gacaagctgc
     1681 gcatggttac gctggataaa ccgcgtctga gtgccgcgat tgaagaagca acacagctgg
     1741 gcgtgaaggt ttttgccctg ccggatggcg atgtcgccgc cagcgtgctg acctgctggc
     1801 aggataatcc ttacgatgtg atgtacacca tcggcggtgc gccagaaggc gtgatttctg
     1861 cctgtgccgt taaagcgtta ggcggggata tgcaggccga gctgattgac ttttgtcagg
     1921 ctaaagggga ttacacggaa aatcggcaga ttgcagagca ggagcgcaag cgttgtaagg
     1981 caatgggcgt tgacgttaac cgtgtgtact cgctcgatga actggtgagg gggaacgata
     2041 tcctctttag cgctacgggc gtgacgggcg gcgagttggt gaacggtatc caacagacgg
     2101 cgaatggggt gcggacgcag acattactga tcggcggcgc ggaccaaacg tgtaatataa
     2161 tagactccct gcattgatgg tgatctatcg gaagaccaat ggcgacgctg acagaagatg
     2221 atgtgcttga gcaactggat gcacaggaca atttattttc atttatgaaa actgcgcatt
     2281 ctattttgct ccaggggata cgccagtttc tgccgtcgct gtttgtcgat aacgatgaag
     2341 agatcgtcga atatgcagtg aagccgttac tcgcccaaag cggcccgctt gacgatattg
     2401 atgttgcgct gcgtttgatt tatgcgctgg ggaaaatgga taaatggctg tatgccgata
     2461 tcacgcactt ttcccagtac tggcattacc tgaacgaaca ggatgaaacg cccggatttg
     2521 ccgatgatat aacctgggat tttatcagca atgtcaacag tataactcgc aatgcgacgc
     2581 tctacgatgc gttaaaagcg atgaagttcg ccgatttcgc cgtctggtca gaggcgcgtt
     2641 ttagcggaat ggtcaaaacg gcgctgacgc tggcagtaac gaccacctta aaggaattaa
     2701 cgccgtgaaa attgaattaa cggtgaatgg gctgaacgtg caagcgcagt accatgatga
     2761 agagatagaa cgcgtgcata aactgttgct gcgtatgctg gcggccttac agacggtaaa
     2821 tccgcagcgg cgtacggtgg tttttctttg cgctccgccg gggacgggta aatctaccct
     2881 aaccaccttc tgggaatatc tcgcgcaaca agacccggag ctgcccgcta ttcaggcgct
     2941 cccgatggat ggttttcacc attacaatag ctggctggac gcgcatcaat tgcgcccctt
     3001 caaaggcgca ccagagacat ttgacgttgc gaaactggcg gaaaatctgc gccaggtggt
     3061 ggaaggggat tgtacgtggc cgcagtacga tcgacaaaag catgatcctg ttgaagatgc
     3121 gttgcacgtt accgcaccac tcgtcatcgt cgaaggaaac tggttgttgc tggacgacga
     3181 gaagtggtgc cagcttgcgc aattttgtga tttctcgatc tttatcaacg cgccagctgc
     3241 tgcgttgcgg gagcgtctgg tggggcgcaa gctggcggga ggggtatcgc tggcagatgc
     3301 agaagccttt tatgaccgta ccgacgggcc gaatgttcgc cgggggctgg aagagagtct
     3361 gcctgcaaat ttgaccttaa tgatgaccgc tacgggtgaa tatcgtctgg tggattaacg
     3421 ttcctgatta caccatcttc cagccgcacc acacgagaga aatgacgccg ggtaaatgcg
     3481 gcgtcatgac tgattgctac cacgctcgtg cctcgctggc ggcacttctc cagccagctt
     3541 tcaaacacgc tcaaccaatt ttcgtcaaaa tcccgactcg gctcgtccag cagtaatagt
     3601 ggcggagaga gtgcttccag gcaggcgacg gcgaccatcc gtcgctgcgc attatgcaga
     3661 tccaacgggt gcgagttggc agtgtctgct aactgacaac attgcagggc agcatgggta
     3721 cgttgagtga tttcatccgc cgggcatttt tgcagtttca aaccaaatgc gatctcgtca
     3781 gcgacggtgc tgtgaaaaag ttggttttcc gcttcctgaa acagtacgcc aacctttgcc
     3841 gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac cgtaccggct
     3901 gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc gccattgtca
     3961 ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca aagacaatcc
     4021 gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt ggctggagcg
     4081 ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg cgatgacgtt
     4141 caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc tgcaacagca
     4201 tttcgctggc ctgcggtgtc aggcggctaa aggcttcatc aagaattaag agctttggct
     4261 gcatcgcgat ggcgcaggcg atgatcacgc gctgtgtctc tccaccggag agtgttgccg
     4321 ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca atccgcgcca
     4381 taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct tcttccacgc
     4441 taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg atggtggcgg
     4501 attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc gtgcctgtta
     4561 tctctcttgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc gtacttttgc
     4621 cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga aagtcataac
     4681 agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac gtaaccatat
     4741 ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta cgcgttgtag
     4801 ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa atgcccgcat
     4861 atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta gcggaataat
     4921 taacgcgggc atagcacgta aacgctgata ccagccctca tccagcggca cgccgcgggc
     4981 acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga ccaataacgg
     5041 cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag cgcgaataaa
     5101 acattgcacg gggacgtact gcattcacaa ctgtgaggtt gaaacaatcg ccagaatcag
     5161 caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc cgcttagcca
     5221 ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga acatcagcca
     5281 gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga aaaaggtcgc
     5341 cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc aggccgctaa
     5401 tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca ttgccggaaa
     5461 atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca ccgctgtaag
     5521 aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca ccaggttttg
     5581 ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga caccgccgaa
     5641 cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa tcacgccgct
     5701 gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac cggagaccag
     5761 gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggtcggtca gcaatcccca
     5821 gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca gtaccgcgct
     5881 aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg ccagttgccc
     5941 gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac tggaaaagtg
     6001 gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa atatgcaact
     6061 cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca aggctaccga
     6121 tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg ccaagcatcg
     6181 tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc agcgtgccgt
     6241 ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc ccggcatagc
     6301 gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ctaggcggca tgaagttgat
     6361 gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg aggcgcagtg
     6421 cgtaaaagcg tgcggaggaa gaatatgagt agggcaatgt catcattgga gtactccgaa
     6481 actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat ataagtcagg
     6541 gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg caaagtggaa
     6601 taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc tttctttgac
     6661 tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt ttcagtcgat
     6721 taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt tccccggacg
     6781 atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag gagatctatg
     6841 accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt tcgtgctttg
     6901 tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact ggcggatgct
     6961 gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc atgggaagta
     7021 cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt gcatgaacgt
     7081 tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga ctgcaccggc
     7141 gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa aaaagtgctc
     7201 ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt caatcaggat
     7261 caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa ttgcataatt
     7321 cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt gaccacaatt
     7381 cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct gcgtcgcacc
     7441 cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg tatcacacga
     7501 ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc aaccataaat
     7561 gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa tgaagtcaac
     7621 ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta tacggaattg
     7681 ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga tggcacccaa
     7741 acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa cgaatggggc
     7801 tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt caggtaagac
     7861 gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt ctgtaattaa
     7921 gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc tgaacgtacc
     7981 agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc cgaccatcga
     8041 actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc gtcctaccga
     8101 aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga aagacaaact
     8161 gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg ctgaaggtga
     8221 actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg acgaaaccct
     8281 gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg gtactgctca
     8341 ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt gcgcaggccc
     8401 gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg ctcgcccgat
     8461 ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg actccctgtc
     8521 taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta tcgcggcaca
     8581 aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag ctaaacgtct
     8641 gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg agttctctga
     8701 aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc agatcctgga
     8761 tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga aaaccattct
     8821 gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta ctgaaatcgt
     8881 ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg acactctggc
     8941 agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg gcggcggcgc
     9001 attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg aagagcgcgc
     9061 taagaagtaa aaaatcacag ggcagggaaa cctgcacttg tttcagcgcg cttttagagc
     9121 acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga gagtgaatcc
     9181 ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc acatgcaact
     9241 tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct aagatttttg
     9301 atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc caggtagcaa
     9361 aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc atcaacgccg
     9421 tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc aacggtggtg
     9481 cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct gctatcctgg
     9541 gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt gttccggtta
     9601 tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt ctgctggacg
     9661 cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac atgatcgacc
     9721 tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg gagcgtatgt
     9781 ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa gaagacggcg
     9841 tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac gttgattacg
     9901 catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc ttcggtaacg
     9961 tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg cgtgattctc
    10021 aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc gtattccacg
    10081 gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc gtagtgaaaa
    10141 tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac tactacaaag
    10201 cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat cagccgaaca
    10261 agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg atcgctcgtc
    10321 tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt cctttctgct
    10381 tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat tcagatgaga
    10441 aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag actgcccgtc
    10501 ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata ttgaatggaa
    10561 gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc taaccaggcg
    10621 ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat cgttggtttg
    10681 attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg taaaatcgat
    10741 gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc ctttacgcta
    10801 atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt actcggtgcc
    10861 gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc cgctggcgtg
    10921 ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg cggcgtagcc
    10981 ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga cggtaaaatt
    11041 atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc ccgcgagcca
    11101 gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga tcaggttaag
    11161 cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg cgaaatgact
    11221 gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt ctggagcaac
    11281 agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg tgaatttgat
    11341 gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg ggtgaaagaa
    11401 gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct ctcttattag
    11461 ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg cgatatagtc
    11521 tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt tcaaggtctt
    11581 gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt gatgaatcag
    11641 ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag cgatttggtc
    11701 ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc gtggttgcta
    11761 gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg cgcttttaaa
    11821 acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca aggcagatgg
    11881 aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta cctggatact
    11941 tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg ttggtttgcg
    12001 ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct cgcagcctgg
    12061 ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt agtgggatgt
    12121 gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc acaagccttg
    12181 ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg cctccagagg
    12241 tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc aaagttatcg
    12301 ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa ttgccggatg
    12361 gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac attgccactc
    12421 ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa caggcagatg
    12481 agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa aaagatatca
    12541 gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa agtatcctga
    12601 aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac aaactgaatt
    12661 ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg tcgctgggcg
    12721 tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat aacgcgattc
    12781 atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta tatagcgtgc
    12841 gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa gccgatgccg
    12901 cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat gatcaggtcg
    12961 atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct gcagcacaat
    13021 aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt gcctcctttt
    13081 tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg tttcatcaag
    13141 gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat ggacattttt
    13201 atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat tgcccgggcg
    13261 gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc gctggaagaa
    13321 caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa taaagccggt
    13381 caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat tgataatgaa
    13441 atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa tacttatccg
    13501 acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt gaccgcccag
    13561 ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca gttagatttt
    13621 attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct gactatcagt
    13681 gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga gcgtcaagag
    13741 caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg ggctaacttc
    13801 gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat tatttatcgt
    13861 ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct gacggtgatc
    13921 ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga tgcgccgcac
    13981 aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta taccgccacg
    14041 atcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc atcgagccgg
    14101 ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga atatgtccgc
    14161 cgggcgcatt ttccagatag cgatgcagat aatcccgata cataggatgt gcacaattat
    14221 caatgatagt gcgggcgcgt tgaagcgggg aaagaccgcg cagatcggcg atcccttgtt
    14281 cggtgatgat cactttgacg ctgtgttcgc tgtgatcaac atggctgcac attggcacga
    14341 cggttgagat cttgccttct ttagcaatcg acggagccat aaagatcgac agatacgcgt
    14401 tgcgttcaaa atcaccgctg ccgccgatgc cgttcatcag atcgaccccg gctacgtgcg
    14461 ttgaatttgc atgcccgtaa atatcaaact ccaggccgac gttcagagcg atgacgccca
    14521 gacgacggat gatttccggg ttattggaaa tctcctgcgg acgcaacaca atgcggctgg
    14581 caaagtaatc catattgtcg taaatcttgc gcagggaatc ggccgagatt gtcaggctgg
    14641 aggcgctggc cccgctgatt ttgccggttt ccagtaaatg caccaccgat tcctgtagca
    14701 cttccgaata catcatgaac ggaggaattt ccgggttttc ccccagacgt gccattaccg
    14761 cattattgat attgcccacg ccactttgca gcggcagaaa ttccggcgga atacgcccat
    14821 gcgccatttc ctgcaataag aacgtgacca cgttatcggc aatctgctgg cacatgggat
    14881 tttgcttatc cagcatatta ccggcgtcgg gcaagttggt ttccacgacg gcgacaatct
    14941 ttttcggatc gatttgcaca tagcgggtac cgacgcgatc cattgcatgg aagatcgaca
    15001 cgctattgcg ccgcggtggc gcgccaggaa tcacaatatc tgccagttct gcaacgcgcg
    15061 gatcgtgata gtgattaagt tcaatgatca ctttcttcgc tcgcagcagc taggtcggcg
    15121 cattaccgat cccgctggtt aaccagactc gaccatccgg tgccagtgcc gatgcttcaa
    15181 tgacggcaac atcaatgtcg ccgaagaaac cgtaattgac catttgcgcc acttcgctca
    15241 aatgcaggtc aacgaaactc accgcgccct gattgatctt tttacgtaaa ctggacgatg
    15301 tttgatatgg cgcacgccag gaaacagcat cggcgtcaga aagtacatcg tcagcggcgg
    15361 cgctgattga cgcccccgtc agcaggcgaa tttgatacgg ctttttggcc tcatgctgtt
    15421 cgttagctct gcgggcaatc gcggcgggta gggctttcgg cgaacccgct ggggtaaagc
    15481 cgctaaatgc caccatgtcg ttatgctgga taatttctgc cgcttcatcg gcggtcatcc
    15541 ttgtccactg agtttccatt tttactcctt cgttcgcagg gattaatgac caacgaaatt
    15601 aggtttacgt ttttcgagga aagcgttcat cccttcctgg taatcttcgc tgtcataaac
    15661 cgcgcggcgc atcccctgaa tacgttcaaa ttcatcggag ttcatggtgt gtgcttcgcc
    15721 cagtacacgc agctcttctt tgataacggc aatggctaac ggcgctttct cagagatgtg
    15781 gtgcgccatt tgtaaggtga aatcttccag ttcttccact tccacaacat ggttgaggat
    15841 gccgacagcc agcgcgcgct gggcggtgat tggcgaagcg gtaaaaatca gctctttgac
    15901 aatgtggaag cccgcgtcgc gggtcaggtt gtgaatgccg accaggttat acgggacgcc
    15961 gaggtttaca ggcgtcattg agaaggttga ggtactggcg gcgatgatca gatcggaact
    16021 catgatcatt tcaaatgcgc caccccaaac actaccttcc accatcgaaa tgatcggttt
    16081 cgggaatttt tggatcatgc gggtgatttg acgcaatgga tcatcatagg agagcggatc
    16141 gcgaccgcca gacggcagtt cgtgaatatc gtgacctgcg gagaagactt tggatccact
    16201 tggcgcccgc aaaatgatac agcgaatttc cggccggttg agatcgctta acgcctgcat
    16261 aagatcatca ataaagactt tacttaaggc attaagtttt cgtccatagt taaactcaat
    16321 gaccgccact ttgttgatag tggcaacgtt cacatactga taagacataa aaattccttt
    16381 aatcaaaata ttgcgtctgg ataaattcac tgagctgccg caggccggtg cgcggtgaga
    16441 gcgtattgtt tttgaccgct aaaagcgtct ggcggtaata gcgatcgaaa tcttcattcg
    16501 cgaacaggtg attcagtact tcttcttcgg tctgcttacg cagccattcc accgattgtt
    16561 gttgccgcac ttgttgtaaa cgaccactgg cagttagcgc ggttttgaag tcgatgatgg
    16621 cgtgccagat ctcatcgatt ccacgttttt ccagtgcgct acaagtcaga ccccgtggct
    16681 gccattcgtc gtatttacgt cgcagaatat gcagggcact ctcgtacata tgccgggcaa
    16741 tggcgacatt ggtatggtta tcgccatcgt ctttgttgat aacgagcaga tcagccactt
    16801 ccatcagccc ttttttaatg ccctgcagat catcgccgcc accggcaatt tgcaacgaga
    16861 taaaacagtc caccatgcgg gcgacttccg tttccgactg cgcgatgcca accgtttcga
    16921 caatcactac gtcataaccc gccgcttcgc ataacagcat taattcccgt gctcgctgac
    16981 tggcaccgcc cagatgaccg gaggagggta ccgggcgaat aaacgccgcc tcggcacgcg
    17041 ccaggtcatt catgcgggtt ttatccccga gaatgctacc gccagtgacc gggctgctgg
    17101 gatcgaccgc aataacagca acctttaatc cctcacgaat caacaacatg ccaaaggctt
    17161 caagaaaggt acttttcccc gcgccgggtg tgccggtaac gcccagtcgc agggcgttac
    17221 cacagaacgg cataatagcg tcaagcagct gtgtacttag cgcctgatga cgtgggtgac
    17281 ggctttccac cagcgtcatg gcttgggcga gcgtggcacg ctcaccctga cgtaagcggc
    17341 gaatactttc tgccagcgtg gcttcattaa tcatgatgct ggcttatcag attcagtacg
    17401 tcgcgcacac tgtcgagcat aggtgtacct ggaccataaa tcgccgccac gccgcgctct
    17461 tgcaggaagg cgtaatcctg cggcggaatg acgccacccg cgaccacgca gatatcttcg
    17521 cgtccccatt ttttcagcgc ttcgaccagt tccgggatca gcgttttatg accggcagcc
    17581 agtgaggatg cgcccactac gtgaacgtcg ttttctacgg ctaggcgggc gatctcttca
    17641 ggtgtagaga acatcgggct taaatctacg tcgaaaccga gatcggaata ggcgctggcg
    17701 atcactttcg cgccgcgatc gtgtccatcc tggcccatct tagcgatcag aatgcgcggg
    17761 cgacgaccat tgtcggcaag gaactgctcc gtttgcgcaa caatggcatc gaactcggag
    17821 gccgatttct cagactgatg atagctttgc gcaatcacgc cggtaacaca ctggcttggc
    17881 accagataac ggtcgaaagc gacttccagc gcatcggaaa tttcacccag ggtggcgcga
    17941 acgcgagcgg cattaacagc ggcagccagc aggttttcgt tatgctgtgc ggcgtgagtc
    18001 agggcgttca acgcggcggt tacggcggca tcatcacggg tggcgcgaat gcgttccagc
    18061 gaagcaattt gctcgttacg caccatcacg ttgtcgatct caagtacatc ggtttcgtct
    18121 tcgtgatcca gtttgtactt gttgacacca acgatgacac gcttgccctg gtcgatcagc
    18181 gactgttcgc gcgctgaggc ctcttcgatc attcgttttg gcagacctgc ttcgatcgct
    18241 ttcgccatgc caccggcttc gtcgatctgt tggataatag ctctggcttg tttgacgatt
    18301 tgatcggtca gcgactcaat gtaataggat ccggccagtg gatcgacggt gtggcagagt
    18361 tctgattctt cctggatgat gatctgggtg ttgcgggcaa tgcgtgctga gaaatcggta
    18421 ggcaaaccaa gcgcttcgtc aaaggcgttg gtatgcagtg actgagtacc gcccagcgtc
    18481 gcagccagtg cttcaatggt ggtgcggata acgttgttat acggatcctg ttcagtcagg
    18541 ctccagcctg aggtctggca gtgggtacgc agcgccagtg atttcggatc ctgtgcgcca
    18601 aatccactga ccgcttcgct ccataaataa cgtgccgcac gcaacatggc gacgttcata
    18661 aacagatcca tgccgatacc gaagaataac gacaggcgag gagcgaagtc atcaattttc
    18721 agtccggcag agattgctgc tttgatgtac tcaatcccat cggcgagcgt aaatgctacc
    18781 tgctgcacgc agttggcacc cgcttccccc atgtggtaac cgctgatact gatggtatta
    18841 aatcgcggca tgttgccgga acaccaggcg atgatgtcgg cgataatgcg cattgacggt
    18901 tttggtgggt aaatataggt gttgcggcag aggtactctt tgagaatatc gttttgaatg
    18961 gtgccggtca gtttatcagg tgtaacacct tgctcttctg cggcgacgat ataaaacgcc
    19021 agtactggta gcactgcgcc attcatggtc atcgaaaccg acattttatc cagcgggatc
    19081 tggtcgaaca ggactttcat atcttccacg gtgtcgatag cgacgcccgc tttgccgacg
    19141 tcacccgcca cgcgtgggtt atcggagtcg tagccacggt gggtggcaag gtcaaacgca
    19201 acggaaagac ctttttgccc ggcggccagg ttacggcgat aaaaagcgtt ggactctttt
    19261 gctgttgaaa aaccagcata ctgacggatg gtccacggtt gggcggtata catagtggca
    19321 cgcgggccac gaacgtaggg cggcaaacca ggaagggtac ctgtcacctc cagattatcg
    19381 agatcggctt cggtatacag cggcttgatg gcgatccctt ccgcggtttg ctgaaccagc
    19441 gagtcgacag ttttctcccg acggctcaat tccttgttgg caagctgttg ccactcctgc
    19501 tcgttagaca ttatttgctc cgtaaaacgc aatcaaaatg agggtatttg cgcttacggt
    19561 gagcttttta cgtgggtttg tcgccagcaa aaaaaatgca ttcagacgtc gttttttgcc
    19621 gcctgagatt tgtctatttt gtgatgtgat tcaaggaata caaaaagaag tgaggacaac
    19681 gcctgatatg tgctatcagg cattatttga tggattaatc ctgacgaagg actttgtgac
    19741 cataatcgag taacgcatca gtgactttac gcatcatgcg gctttcagga gcaaagcggt
    19801 gccagtagag catccgtcgt tgaaatagcc caggcgttaa gtcaatcagt tcaccgctgg
    19861 ccagctcttt ctcgatttgc aggtgcggga tcatacagca ggtggtgccc tggcgagcaa
    19921 gttgtacgaa cgcttctgaa gaattaacga tatggcaggg tacgctgcca ggaggaagat
    19981 cgaagttttg ctgcaaaaag gcctggtgca tatcgtcaag atggtcaaac gcgaccactg
    20041 gcgctttcaa taatgccgaa cgcgttacgc cgttaggaaa atatttttcg gcaaacggtt
    20101 ttgagctgac gaacagatag tcgagcgcac cgagtttatc gacaagacaa ctcggcagcg
    20161 cctgatgttg aatactcacc gcgccgacca cttcgccgcg gcgcagacgt tcctgagtac
    20221 gggtttcatc ttctacctgc aagttgaggc ggataggcga atcagccaac acaggagcca
    20281 gtgccggaag caaccacgtc gccagactgt cggcgttgac cgccagtgaa agcagcagcg
    20341 gagtcgaacc ggtttgttca tcgcccagcc actcttcttc cagcaactcc acctggcgca
    20401 gcagtgccag cagtttttgc ccttgttccg tcgggcgcgg cggtacggta cgcaccaaca
    20461 gcggctgccc gaacatattt tccagttgct taatgcgctg tgagacggct gattgtgtaa
    20521 tgcacagctt ttgtgccgcg cgctcaaatc ctcgttcacg tatcaccgca tccagtgcct
    20581 gtaatgttct gtagtccggg cgtttcattg ctgttgtctg ctcctggtaa atgctttttc
    20641 ctgcactatg acacaatttt gtgtcaggtt gcatataccg aagccgtcgg cgggtaacat
    20701 ttgtatcaat ggttaaacgc gcagcttaaa agatcaggga tttgcgctat tgcatttcgc
    20761 ttagcgactt cgcgccctgg ttatgttgaa tggcgtggcg ttattgcctc tgttcgcacc
    20821 gtggagcggc atacccagac cgttgtatgc tttatttgcg gggcgaaagg ggatgcccgc
    20881 cattgcgcga tattttatgg atgagttaac cacccggctt gccaacgggg tctgaatcgc
    20941 tttttttgta tataatgcgt gtgaaatttc ataccacagg cgaaacgatc atgacgcagg
    21001 atgaattgaa aaaagcagta ggatgggcgg cacttcagta tgttcagccc ggtaccattg
    21061 ttggtgtagg tacaggttcc accgccgcac actttattga cgcgctcggc acgatgaaag
    21121 gccagattga aggggccgtt tccagttcag atgcttccac tgaaaaactg aaaagcctcg
    21181 gcattcacgt ttttgatctc aacgaagtcg acagccttgg catctacgtt gatggcgcag
    21241 atgaaatcaa cggccacatg caaatgatca aaggcggcgg cgcggcgctg acccgtgaaa
    21301 aaatcattgc ttcggttgcg gaaaaattta tctgtattgc cgacgcctcc aagcaggttg
    21361 atattctggg taaattcccg ctgccagtag aagttatccc gatggcacgt agtgcagtgg
    21421 cgcgccagct ggtgaaactg ggcggtcgtc cggaataccg tcagggcgtg gtgaccgata
    21481 atggcaacgt gatcctcgac gtccacggca tggaaatcct tgacccgata gcgatggaaa
    21541 acgccataaa tgcgattcct ggcgtggtga ctgttggctt gtttgctaac cgtggcgcgg
    21601 acgttgcgct gattggcaca cctgacggtg tcaaaaccat tgtgaaatga tctgacgggg
    21661 gaacctcccc cgttaaaaaa attctcttca ttaaatttgg tgacatgtgt cacgctttta
    21721 ccaggcaatt gtcgattgct ctaaataaat cctctaaacc agcatattca tccaagaatt
    21781 acctttgcgt gatatttcct caacatcgcg acgcaaacgt tcatattgcc gcaatattat
    21841 tttttgatat gttgaaaggc ggatgcaaat ccgcacacaa catttcaaaa gacaggattg
    21901 ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg gtagaaggcg
    21961 tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc gaatttcaca
    22021 aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac ttcatcggcc
    22081 tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaaa ctggtcgcta
    22141 ttggctgttt ctgtatcgga acaaaccagg ttgatctgga tgcggcggca aagcgcggga
    22201 tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg gtgattggcg
    22261 aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac cgtggcgtgt
    22321 ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt atcatcggct
    22381 acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg tatgtttact
    22441 tttatgatat tgaaaacaaa ctgccgctgg gcaacgccac tcaggtacag catctttctg
    22501 acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg tccaccaaaa
    22561 atatgatggg cgcgaaagag atttcgctaa tgaagcccgg ctcgctgctg attaatgcct
    22621 cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc aaacatctgg
    22681 cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat ccatttacct
    22741 ctccactgtg tgaattcgac aacgtccttc tgacgccaca cattggcggt tcgactcagg
    22801 aagcgcagga gaatatcggc ttggaagttg cgggtaaatt gatcaagtat tctgacaatg
    22861 gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat ggtggtcgtc
    22921 gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac aaaatcttcg
    22981 ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag atgggttatg
    23041 tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag gcaatgaaag
    23101 ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct gaaaattaac
    23161 gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc cgcgttgcgc
    23221 attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg cgttgtgttt
    23281 atttaatggg gatttacgat ggcgggacag catggggctg taacggtaaa gccctcaccg
    23341 aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg attgtactgt
    23401 tactgataag tttcggagct tattaacagt cagtctcaag ggaggagtca tcctccctta
    23461 cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga tgcggggtgc
    23521 atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact gccgtaatag
    23581 atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgagcac gctcgaagaa
    23641 aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg atagctgggc
    23701 agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga gaagggttat
    23761 gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag cttattaaca
    23821 gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta ggttaaagaa
    23881 tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca gactttcgac
    23941 ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt ttccaccaac
    24001 tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt ttgtaaggtc
    24061 cgatcataaa aaccaccgcc cattcccaga cgctgaccgt actcatcaaa ggcgaccagc
    24121 ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc cagttttggc
    24181 tcatggatct tcaacctgtt catcaccagt tcgctttgcg gatggtaatt caggaacagc
    24241 aaattaccgg cactaaaggg atgcaaaact ggaagatata cgcgcttacc ggcgcgccag
    24301 agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa tacagcgacc
    24361 gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg ttgacccatt
    24421 tccagctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt gcggatttct
    24481 tgtcgggata atgtcagtgg gagctctggt agttgcgtca tggctggtgc cagataagaa
    24541 gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc aaggtgaatg
    24601 tgtcgtcgca gttttaaggc ttctcggacg aaccgagcat gctcaccaac cgcggagcgc
    24661 cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga acatctcaga
    24721 gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat tcaaagtttt
    24781 ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg gtctgctgca
    24841 gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtctttgct ttttcttgcg
    24901 ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta tttgtgactc
    24961 tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc tgattcaacg
    25021 catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt tggatatcga
    25081 cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg tctttagacc
    25141 catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa gcccttttct
    25201 ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg acgacgaatg
    25261 cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta tctgaaccaa
    25321 caagggacgg gtctgacccc tgccgagatg catggtttaa tcagtgggat gatatgtggc
    25381 ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga aggcatggct
    25441 ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag cgatgccctg
    25501 caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt cagcgttttc
    25561 gatcgggctg atgcactggc aggttgggtc aatcacttcc tgcttggtct tggcgttacg
    25621 caaccgaagc tggacaaagt gaccggcgaa accggtgaag ccatcgacga tctgcgtaac
    25681 atcgcgcagc tgggctacga cgaagacgaa gatcaggaag agcttgaaat gtcgcttgaa
    25741 gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt tactcatccg
    25801 caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa aacgtaagga
    25861 gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg ccctggtgga
    25921 gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa cacgtagcgc
    25981 cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg gctttaacga
    26041 accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca gcgttctgtt
    26101 taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag gccaggatgc
    26161 cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca atcagcaact
    26221 ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat atgcatatgc
    26281 tgatgaaatc gtgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc aaaatctcac
    26341 cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc tgttcaaatc
    26401 gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg cacatacacg
    26461 ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg aaattcacca
    26521 cgaatttaac cgccacggtg cgcgctatcc ttcctataac accattgtag gcagcggtga
    26581 aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg gtgacctggt
    26641 gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc gcaccttccc
    26701 ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc tggagtctct
    26761 cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca ctggtgaagt
    26821 ggtgcgcatc atggttagcg gcctggtaaa actcggcatc ctgaaaggta atgttgatga
    26881 actgatcgct cagaacgccc atcgtccttt ctttatgcat ggccttagcc actggttagg
    26941 actggatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc tggaaccggg
    27001 gatggtactg accgtagagc ctggtctgta tatcgcgccg gatgcggatg tgccagaaca
    27061 atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa ccggcaacga
    27121 aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga tggctgctgc
    27181 gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac gctggcgctg
    27241 gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc gactgtgcca
    27301 gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc gggtacctgt
    27361 cagcaactgg cgcgcatcgg cgtctggcaa tctctggctg attgcgcaac cgccatcact
    27421 accgtgcatg tcagcgatcg tggtcatgct ggattcgtca ccctcgccgc agaagattac
    27481 caactggcgg cgctggggca ggttgtcgaa ttgcataatg tcgggcaacg gctgtttgca
    27541 ttgctgcgaa aagcacctgg cgtaatgctg cattgccccg atcgcgtagc taacgttgcc
    27601 cgtactcaga gtcatgttga agtgacgctg gagagcggcg agatgctgac gggccgcgtg
    27661 ctgatagccg ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt tgattggcag
    27721 caggagcctt acgaacaact ggcagtgatt gccaacgttg ctacttctgt tgcgcacgaa
    27781 ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc gatgtctgac
    27841 ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt gctgtcgtgg
    27901 agcgacgaga agttttgccg tgaactccag tctgcctttg gctggcgact ggggcaaatt
    27961 acccacgccg gtaaacgcag tgcttatccg ctggcattaa cccgcgccgc cagaccgatt
    28021 acccatcgca ctgtgctggt gggcaatgcg gcgcaaactc tgcacccgat tgccgggcaa
    28081 gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac tcaggcgcat
    28141 gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca gcgtcgacag
    28201 agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt tgccaaccgt
    28261 tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt attcaccccg
    28321 gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag gcggttttaa
    28381 ggaataaaca atgcaaagtg ttgatgtagc cattgttggc ggcggcatgg tggggctggc
    28441 ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc agcgcgtacc
    28501 ggaacctctg gcggtagatg cactaccaca actgcgcgtt tcggctatca atgccgccag
    28561 cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctcgctcgta gggccagttg
    28621 ttatcacggc atggaagtgt gggacaaaga cagttttggt cacatctcgt ttgacgatca
    28681 aagcatgggc tatagccatc ttgggcacat cgttgaaaac tcagtgattc actacgcgct
    28741 gtggaacaaa gcgcagcagt cgtcagatat tactctgttg gcccccacag aattacagca
    28801 ggtcgcctgg ggagaaaatg aaaccttcct gacgctgaaa gatggcagca tgttaacggc
    28861 gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag cagatattcc
    28921 gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca cggaagaacc
    28981 gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct ttttaccgct
    29041 tagcgatccg catctttgct cgattgtctg gtcactgtcg ccagaggaag cgcagcggat
    29101 gcagcaggca agtgaagacg aatttaatcg cgcgttaaat atcgcttttg ataatcgcct
    29161 tggcttatgc aaggttgaga gcgcgcgtca ggtgttcccg ctgacggggc gttatgcgcg
    29221 ccagtttgcc gcacaccgtc tggcgctggt gggcgacgcc gcgcatacca ttcacccact
    29281 ggcggggcag ggcgttaatc tcggatttat ggacgccgca gagctgattg ctgaactgaa
    29341 acggttgcat cgtcagggta aagacatcgg gcagtacatt tacctgcgcc gctatgaacg
    29401 tagccgcaag cacagcgcgg cgctgatgct ggctggtatg cagggttttc gcgatctctt
    29461 ttccggcgct aatccggcga aaaaactgct gcgtgatatt ggtttgaaac tggccgacac
    29521 gcttcctggc gttaagccgc aacttatccg ccaggcaatg ggattaaacg atttgcctga
    29581 atggctgcgt taaaaatttc tcctctgttg tttatttgat acccatcaca ctttcatctc
    29641 ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac ctccgttttc
    29701 gcattatatt ttctaatacc atgatttttt gatttagtgt tttttgacat ttttttagct
    29761 cttaatattg tcttattcaa attgactttc tcatcacatc atctttgtat agaaattggt
    29821 gtattttttg gttttttatt ctgtcgcgat ttttgcattt tttaaccata agctaatgtg
    29881 atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag gcaaaagaga
    29941 acgattgcgt tggggaccgg gagtggctcc gatgctgggt tttgtggtga taatttcacc
    30001 atgaaaaagt tgtcagcccc gcttattcaa tgaggacaag atggcacaac agactccttt
    30061 gtacgaacaa cacacgcttt gcggcgctcg catggtggat ttccacggct ggatgatgcc
    30121 gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta cgtaccgatg ccggaatgtt
    30181 tgatgtgtcg catatgacca tcgtcgatct ccgcggcagc cgcacccggg agtttctgcg
    30241 ttatctgctg gcgaacgatg tggcgaagct caccaaaagc ggcaaagccc tttactcggg
    30301 gatgttgaat gcctctggcg gtgtgataga tgatctcatc gtctactact ttactgaaga
    30361 tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa gacctctcct ggattaccca
    30421 acacgctgaa cctttcggca tcgaaattac cgttcgtgat gacctttcca tgattgccgt
    30481 gcaagggccg aatgctcagg caaaagctgc cacactgttt aatgatgctc agcgtcaggc
    30541 ggtggaaggg atgaaaccgt tctttggcgt gcaggtgggc gatctgttta ttgccaccac
    30601 tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc aatgaaaaag cggccgattt
    30661 ctggcgtgcg ctggtggaag cgggcgttaa gccctgcggt ctgggggcgc gtgacacgct
    30721 gcgtctggaa gcgggcatga atctttatgg tcaggagatg gacgaaacta tttctccttt
    30781 agccgccaac atgggctgga ctatcgcctg ggaaccgaca gatcgtgact ttatcggtcg
    30841 tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa ctggttggtc tggtgatgac
    30901 cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt accgatgcgc agggcaacca
    30961 gcatgaaggc attatcacca gcggtacttt ctccccgacg ctgggttaca gcattgcgct
    31021 ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg caaattcgca accgtgaaat
    31081 gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc aaagccgtcg cgtgatttac
    31141 ttttttggag attgattgat gagcaacgta ccagcagaac tgaaatacag caaagaacac
    31201 gaatggctgc gtaaagaagc cgacggcact tacaccgttg gtattaccga acatgctcag
    31261 gagctgttag gcgatatggt gtttgttgac ctgccggaag tgggcgcaac ggttagcgcg
    31321 ggcgatgact gcgcggttgc cgaatcggta aaagcggcgt cagacattta tgcgccagta
    31381 agcggtgaaa tcgtggcggt aaacgacgca ctgagcgatt ccccggaact ggtgaacagc
    31441 gaaccgtatg caggcggctg gatctttaaa atcaaagcca gcgatgaaag cgaactggaa
    31501 tcactgctgg atgcgaccgc atacgaagca ttgttagaag atgagtaacg gctttattcc
    31561 tcttctgcgg gagaggatca gggtgaggaa aatttatgcc tcaccctcac tctcttcgta
    31621 aggagagagg ttcacaattc actgcacgtt tcaggaacca tcgctcatga cacagacgtt
    31681 aagccagctt gaaaacagcg gcgcttttat tgaacgccat atcggaccgg acgccgcgca
    31741 acagcaagaa atgctgaatg ccgttggtgc acaatcgtta aacgcgctga ccggccagat
    31801 tgtgccgaaa gatattcagc ttgcgacacc accgcaggtt ggcgcaccgg cgaccgaata
    31861 cgccgcactg gcagaactca aggctattgc cagtcgcaat aaacgcttca cgtcttacat
    31921 cggcatgggt tacaccgccg tgcagctacc gccggttatc ctgcgtaaca tgctggaaaa
    31981 tccgggctgg tataccgcgt acactccgta tcagcctgaa gtctcccagg gccgccttga
    32041 agcactgctc aacttccagc aggtaacgct ggatttgact ggactggata tggcctctgc
    32101 ttctcttctg gacgaggcca ccgctgccgc cgaagcaatg gcgatggcga aacgcgtcag
    32161 caaactgaaa aatgccaacc gcttcttcgt ggcttccaat gtgcatccgc aaacgctgga
    32221 tgtggtccgt actcgtgctg aaacctttgg ttttgaagtg attgtcgatg acgcgcaaaa
    32281 agtgctcgac catcaggacg tcttcggcgt gctgttacag caggtaggca ctaccggtga
    32341 aattcacgac tacactgcgc ttattagcga actgaaatca cgcaaaattg tggtcagcgt
    32401 tgccgccgat attatggcgc tggtgctgtt aactgcgccg ggtaaacagg gcgcggatat
    32461 tgtttttggt tcggcgcaac gcttcggcgt gccgatgggc tacggtggcc cacacgcggc
    32521 attctttgcg gcgaaagatg aatacaaacg ctcaatgccg ggccgtatta tcggtgtatc
    32581 gaaagatgca gctggcaata ccgcgctgcg catggcgatg cagactcgcg agcaacatat
    32641 tcgtcgtgag aaagcgaact ccaacatttg tacttcccag gtactgctgg caaacatcgc
    32701 cagcctgtat gccgtttatc acggcccggt tggcctgaaa cgtatcgcta accgcattca
    32761 ccgtctgacc gatattctgg cggcgggcct gcaacaaaaa ggtctgaaac tgcgccatgc
    32821 gcactatttc gacaccttgt gtgtggaagt ggccgacaaa gcgggcgtac tgacgcgtgc
    32881 cgaagcggct gaaatcaacc tgcgtagcga tatcctgaac gccgtgggga tcacccttga
    32941 tgaaaccacc acgcgtgaaa acgtaatgca gcttttcagt gtgctgctgg gcgataacca
    33001 cgggctggag atcgacacgc tggacaaaga cgtggctcac gacagccgct ctatccagcc
    33061 tgcgatgctg cgcgacgacg aaatcctcac ccatccggtg tttaatcgct atcacagcga
    33121 aaccgaaatg atgcgctata tgcactcgct ggagcgtaaa gatctggcgc tgaatcaggc
    33181 gatgatcccg ctgggttcct gcaccatgaa actgaacgcc gccgccgaga tgatcccaat
    33241 cacttggacg gaatttgccg aactgcaccc gttttgcccg ccggaacagg ctgaaggtta
    33301 tcagcagatg attgcgcagc tggctgactg gctggtgaaa ctgaccggtt acgacgccgt
    33361 ttgtatgcag ccgaactccg gcgcacaggg cgaatacgcg ggcctgctgg cgattcgtca
    33421 ttatcatgaa agccgcaacg aagggcatcg cgatatctgc ctgatcccgg cttctgcgca
    33481 cggaactaac cccgcttctg cacatatggc aggaatgcag gtggtggttg tggcgtgtga
    33541 taaaaacggc aacatcgatc tgactgatct gcgcgcgaaa gcggaacagg cgggcgataa
    33601 cctctcctgt atcatggtga cttatccttc tacccacggc gtgtatgaag aaacgatccg
    33661 tgaagtgtgt gaagtcgtgc atcagttcgg cggtcaggtt taccttgatg gcgcgaacat
    33721 gaacgcccag gttggcatca cctcgccggg ctttattggc gcggacgttt cgcacctcaa
    33781 cctgcataaa actttctgca ttccgcacgg cggtggtggt ccgggtatgg gaccgatcgg
    33841 tgtgaaagcg catctggcac cgtttgtacc gggtcatagc gtggtgcaaa tcgaaggcat
    33901 gttaacccgt cagggcgcag tttctgcggc accgttcggt agcgcctcta tcctgccaat
    33961 cagctggatg tacatccgca tgatgggcgc agaagggctg aaaaaagcaa gccaggtggc
    34021 aatcctcaac gccaactata ttgccagccg cctgcaggat gccttcccgg tgctgtatac
    34081 cggtcgcgac ggtcgcgtgg cgcacgaatg tattctcgat attcgcccgc tgaaagaaga
    34141 aaccggcatc agcgagctgg acattgccaa gcgcctgatc gactacggtt tccacgcgcc
    34201 gacgatgtcg ttcccggtgg cgggtacgct gatggttgaa ccgactgaat ctgaaagcaa
    34261 agtggaactg gatcgcttta tcgacgcgat gctggctatc cgcgcagaaa ttgaccaggt
    34321 gaaagccggt gtctggccgc tggaagataa cccgctggtg aacgcgccgc acattcagag
    34381 cgaactggtc gccgagtggg cgcatccgta cagccgtgaa gttgcggtat tcccggcagg
    34441 cgtggcagac aaatactggc cgacggtgaa acgtctggat gatgtttacg gcgaccgtaa
    34501 cctgttctgc tcctgcgtac cgattagcga ataccagtaa ttcactgatt cgagtatttt
    34561 ctaaaggcgc ttcggcgcct ttttagtcag atgacaaagt acaaaagtgc tcagacagtc
    34621 ccctcgcccc tttggggaga gggttagggt gaggggaaca ggccggcact ggcgcgaata
    34681 tttaccctca ccccgaccct ctccctgaaa gggcgagggg gaaaagcgtg ccaacattga
    34741 agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaattaatt tttctcggaa
    34801 aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg gcatcgggcg ggcaactgca
    34861 ttactgttgg cgcaagaagg gtatacggtg gcggttaatt atcagcaaaa cctccacgcg
    34921 gcgcaggaag tgatgaactt aataatgcaa gccggtggca aagcattcgt gctccaggcg
    34981 gatatcagcg acgaaaacca ggtcgttgcg atgtttacag caatcgatca gcacgatgaa
    35041 ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac cgttgaaaac
    35101 cttaccgcag agcgaatcaa ccgagtactt tccaccaacg tgacgggata ttttctctgc
    35161 tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg cgctatcgtc
    35221 aatgtctctt cggtggcctc acggttgggt tcgccagggg aatatgttga ttacgcggca
    35281 tcgaaagggg cgattgattc gttaaccacc ggactatcgc tggaagtcgc cgcgcagggg
    35341 atccgcgtta actgcgtgcg gccagggttt atttataccg aaatgcacgc cagcggcggc
    35401 gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg tggacaggca
    35461 gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta cgtcacggga
    35521 agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc ggatgcaaca
    35581 agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc aatcacctct
    35641 ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc gtcgtcatgt
    35701 ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa tgacacgcag
    35761 tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt cactgctttt
    35821 ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt gatgctgcca
    35881 tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag cggcctctga
    35941 taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat ctgccagccc
    36001 cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga gatcgcatcg
    36061 ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc aagataatcg
    36121 caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatatt aaatccgcga
    36181 cgttcccact cgttcaatac ataggacggg taatagccgc atagctgcac atcggtaaag
    36241 acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt acatgagtag
    36301 ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt aatgcgacgc
    36361 gcggctttca ctgccagggc gctggcgaca aactggtgat gcagcacctg gtacatcgtc
    36421 tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc aaacagcggc
    36481 gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt gactttatgc
    36541 ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgaa tttacggttg
    36601 gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga gagggttatc
    36661 accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa cttcagccct
    36721 tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc gatggaagtc
    36781 cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta gtgaccataa
    36841 aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt ggtgatttcg
    36901 cgcggcacgc cgtgtgcgcc acaggtcaga acgtcacaaa tgctcggtcc tttgccgcct
    36961 ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa gaaatctttc
    37021 ggtaaggtga gttttttcac tatcataggc tccagatcag tcaattctta caggaaatag
    37081 taccatgcag ccaaacgaca tcactttttt caacgtttcc aggatgacat tctggctggg
    37141 cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt
    37201 cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac cgcaacctca
    37261 accgtaacgc tggatacact gacggaaaaa catgcagagc aggaaaatat gaccctgcct
    37321 gaactgaaaa aggtcattgc tgacatctat cccggtcaga cacaatttta tatgattgaa
    37381 tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt taatttcatg
    37441 atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt tcactggaac
    37501 tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctctgg agtaagttat
    37561 ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg ccaacagcgt
    37621 cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac tggttcaggc
    37681 ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg gcggcagtat
    37741 gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac gggcaaaaat
    37801 gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa cctacacgcc
    37861 gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg ttatctggag
    37921 cctagcattg ctggggattc tgttcaaact gaccatcgcg caccgattca aaattttatc
    37981 tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg aaatggcagt
    38041 taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt attcgctcgg
    38101 ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc acggcttcgt
    38161 gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc aggcgtaatg
    38221 ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcatc ggagcgcaac
    38281 cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt tgtgataaag
    38341 gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata aatgcctgat
    38401 gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc agtgtattcg
    38461 catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg acgacctgta
    38521 ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta cgacgccagt
    38581 tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg gcactacctg
    38641 ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc accatctctt
    38701 gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga ttggtcgcct
    38761 gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct tcaatgttta
    38821 atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca
    38881 tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt tcaaaccata
    38941 gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt tcgctaaaga
    39001 gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt
    39061 ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc agctgcggtt
    39121 cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac agacgtaaat
    39181 tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga tcttctgcca
    39241 tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc gcgccagtaa
    39301 tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg
    39361 taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt gcttaacggc
    39421 gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca ctacgcgatc
    39481 tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg tgaattaatt
    39541 tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa
    39601 tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa caaagcccga
    39661 attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat gtcgtttttc
    39721 gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg tctgctggaa
    39781 tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc cgatgcagaa
    39841 ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc tgtggcaatc
    39901 tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg ggctggttgc
    39961 cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga tggtgttact
    40021 ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc gccaggggga
    40081 aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa
    40141 agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg gcggtaaacg
    40201 gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg atttacggcg
    40261 gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat gcgctaagcc
    40321 gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg caccagctct
    40381 gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga cccacgaaca
    40441 gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc gttggccagt
    40501 agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg agcgcatcga
    40561 ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata attttacctt
    40621 caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg atttcaccaa
    40681 aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat ttcggggagt
    40741 cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg attttttctg
    40801 ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc ataacgtaag
    40861 cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta gtcacggcac
    40921 aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac aggagctggc
    40981 acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg aaaatacgtt
    41041 gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac
    41101 gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc tggagggcgg
    41161 gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt tccagtatct
    41221 ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc
    41281 ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac aggcgccatt
    41341 aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt atgctaccgg
    41401 gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc gtcagggcgt
    41461 agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg aagaggcggt
    41521 ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat
    41581 tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct tctggcaccg
    41641 cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt
    41701 gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg tggtgcagat
    41761 gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg ctaccgagcg
    41821 tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag
    41881 atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag gctttgctca
    41941 ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat
    42001 tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg tgttgtacat
    42061 caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg gcacggctcc
    42121 ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat
    42181 cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata ttacctgtgg
    42241 ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga tcaccgtgcg
    42301 ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa tgaaagctat
    42361 ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc
    42421 accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc agcttggcgt
    42481 tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt accagccgcc
    42541 gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta aataattcgc
    42601 gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct
    42661 gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag tgcgcaagaa
    42721 ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt cgaaaaggcc
    42781 gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc
    42841 gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc gcttggttgc
    42901 agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt aagcccggaa
    42961 gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga taacggtatt
    43021 tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat tgttaccgat
    43081 caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc taacttgcgc
    43141 gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta tctgatgctg
    43201 gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat cgcaattcct
    43261 aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt cgtgccgctg
    43321 gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc cggaaagtgc
    43381 cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa actagccgcc
    43441 agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact ggatgatatg
    43501 tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat
    43561 gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc
    43621 ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg
    43681 tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat caaagagcgt
    43741 tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa aggttccggt
    43801 cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac actctacccc
    43861 ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat
    43921 aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct ggccccttcg
    43981 ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat gaccatggaa
    44041 gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga gccgctgttt
    44101 gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg
    44161 gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt cgataccgct
    44221 ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga tatcaacgag
    44281 tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat ttagcgtcat
    44341 cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc gcattttgac
    44401 tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa tcgcattcag
    44461 gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg ccaagaaaga
    44521 gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg aacccgaacg
    44581 cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca
    44641 aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag aagctgacga
    44701 cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa aactggcgca
    44761 gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt acctcgatat
    44821 tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg agcgtatgta
    44881 tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt cggaaggtga
    44941 agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt acggctggct
    45001 gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg
    45061 tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg atattgatat
    45121 cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca
    45181 gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg ggatcgtgac
    45241 ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga agcagatgaa
    45301 agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga
    45361 taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg atgactcccg
    45421 cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc tggacggcag
    45481 cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca acatgtctga
    45541 acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga aaacgcgtcg
    45601 tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga
    45661 tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag aactggaagc
    45721 gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta tgggtaaagc
    45781 gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga
    45841 tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg acatcctcgg
    45901 cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact gtaccgagct
    45961 gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct tgcaggatca
    46021 ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat cccgtaacac
    46081 ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga accgcggctt
    46141 tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt
    46201 tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc cggaactgta
    46261 cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg
    46321 taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac tctacatggc
    46381 ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta ctctggcaca
    46441 ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact tcggtaaacc
    46501 gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa ccgacatggc
    46561 ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta tccacgttga
    46621 gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg cagaagcaca
    46681 tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc tggcgcgtcg
    46741 taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat
    46801 cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct tcctggatca
    46861 ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag attacgttac
    46921 cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg accgtatggt
    46981 aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc
    47041 ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt ttttatttaa
    47101 gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc atcaccatcc
    47161 acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct aaatcacacc
    47221 attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa
    47281 ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg tagcgaaagt
    47341 caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg caacggcgga
    47401 tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag ttagtccaca
    47461 cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc cccttggcat
    47521 taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt gcggcatact
    47581 tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt tccgtttgca
    47641 taatttctca catgtaattc tgatcactca tcattataga taattgaaga gtgaaatgat
    47701 taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac
    47761 catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg caacacagtc
    47821 agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag cgcgcattaa
    47881 aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga agccacatac
    47941 gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg tcagcatgat
    48001 gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg agcataatgc
    48061 cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg tgagagagcg
    48121 tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa cggttggtgg
    48181 tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg aacatcacta
    48241 gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc atttttggca
    48301 ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg ctatgaacgc
    48361 gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag ctgttaaacg
    48421 tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata tcgtgtgaag
    48481 agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac tcgataaaca
    48541 cgatgattaa cacggcagtc atggtcagaa tggaaacggg atcgaaaatc ggcataccaa
    48601 acgacatcgg ggtgacaatc gcaaaccacg aggcatcatg tagcccggat aaattgactt
    48661 cattcatcat ccacgaaagt aaaaagccaa atacaatccc cagtaatacg gcgacgttgg
    48721 acataaatcc tttcgcatag cgagtaatga gcaagataaa aattaagacg gcaaaggaga
    48781 tacctaaata aacgggatta ccatattgcg gattcccttt acctccagcg gcccagtcaa
    48841 tacccacctg aatgatgcta agcccaatag aagtaataac cacaccggta accagtggcg
    48901 ggaataaagg catcaagcga ccaataagtg gcgctaataa tgtggtgata aaacccgcgg
    48961 cgatagtggc accaaatatc cccagcaggc cgatatccgg gttcatccca atggctatca
    49021 ttggtgttac agcagcaaag gtcaccgaca taatcaccgg caggcggatc cccataaagc
    49081 ggccgatacc gatacattgc aataatgtga cgatcccgca gcaaaagaga tccgagctaa
    49141 tgagcatcgc aatagcatct tttgagaggc ccagtcggtc accaatcata agaggaacag
    49201 cgactgcacc tgcgtacatg acaaggacgt gttgcagacc gagtatgatc agctttcctg
    49261 gtgataatat gcggtcaacc tcatcagttg ggcggtcttg cccagaagat gagggaagtt
    49321 gggaatctat ggcgctcatc agtgtttctc ctttcgcccg ccagaataaa aagcaaccgt
    49381 ttacgttaat gaaactgtct aaacacatgc tcgtggagtg ccagtttttg cgaggaaggg
    49441 gagttagaga caggaattgc ggaggcaatc acataatcgt gcttaaacca cgaaaatgac
    49501 aagagaaact gacaatggaa ttatctgtaa ttttctcata ttgatatttc attaatcatt
    49561 ctggcatgtc gcactctcgc atttaatcgt ttttatctgg atagcgctct tttgatcggc
    49621 aatgttgaaa taatgaaagc ggcatagatt gaggggcaat aatttgaact cagtcgcaaa
    49681 attataatta aaacggtcat gctacctaat atatgcccgt ttattgcatc gcgaaatttg
    49741 agaagacata actcattaaa tgaaagggat gcaaatgaat acgtttatcg ctgctgaagc
    49801 tgcggaatgt ataggctgtc a
//
LOCUS       DAJVQR010000020        49535 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVQR010000020 DAJVQR010000000
VERSION     DAJVQR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..1155)
                     /gene="setB"
                     /locus_tag="OQM52_001226"
     CDS             complement(<1..1155)
                     /gene="setB"
                     /locus_tag="OQM52_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1563154.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1523..2653
                     /gene="fruB"
                     /locus_tag="OQM52_001227"
     CDS             1523..2653
                     /gene="fruB"
                     /locus_tag="OQM52_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1563155.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2653..3591
                     /gene="fruK"
                     /locus_tag="OQM52_001228"
     CDS             2653..3591
                     /gene="fruK"
                     /locus_tag="OQM52_001228"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1563156.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3608..5299
                     /gene="fruA"
                     /locus_tag="OQM52_001229"
     CDS             3608..5299
                     /gene="fruA"
                     /locus_tag="OQM52_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1563157.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5723..6664
                     /locus_tag="OQM52_001230"
     CDS             5723..6664
                     /locus_tag="OQM52_001230"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1563158.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6652..7590
                     /gene="psuG"
                     /locus_tag="OQM52_001231"
     CDS             6652..7590
                     /gene="psuG"
                     /locus_tag="OQM52_001231"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1563159.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7684..8934
                     /locus_tag="OQM52_001232"
     CDS             7684..8934
                     /locus_tag="OQM52_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1563160.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9005..9699)
                     /gene="yeiL"
                     /locus_tag="OQM52_001233"
                     /pseudo
     CDS             complement(9005..9699)
                     /gene="yeiL"
                     /locus_tag="OQM52_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9830..10771
                     /gene="rihB"
                     /locus_tag="OQM52_001234"
     CDS             9830..10771
                     /gene="rihB"
                     /locus_tag="OQM52_001234"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1563161.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10871..12121
                     /locus_tag="OQM52_001235"
     CDS             10871..12121
                     /locus_tag="OQM52_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1563162.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12177..13265)
                     /locus_tag="OQM52_001236"
     CDS             complement(12177..13265)
                     /locus_tag="OQM52_001236"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1563163.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13268..14125)
                     /gene="nfo"
                     /locus_tag="OQM52_001237"
     CDS             complement(13268..14125)
                     /gene="nfo"
                     /locus_tag="OQM52_001237"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1563164.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14199..15248)
                     /locus_tag="OQM52_001238"
     CDS             complement(14199..15248)
                     /locus_tag="OQM52_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1563165.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15347..16228
                     /locus_tag="OQM52_001239"
     CDS             15347..16228
                     /locus_tag="OQM52_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1563166.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16434..17903
                     /gene="lysP"
                     /locus_tag="OQM52_001240"
     CDS             16434..17903
                     /gene="lysP"
                     /locus_tag="OQM52_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1563167.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18195..20186
                     /gene="cirA"
                     /locus_tag="OQM52_001241"
                     /pseudo
     CDS             18195..20186
                     /gene="cirA"
                     /locus_tag="OQM52_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20218..21053)
                     /gene="yeiG"
                     /locus_tag="OQM52_001242"
                     /pseudo
     CDS             complement(20218..21053)
                     /gene="yeiG"
                     /locus_tag="OQM52_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21311..21979
                     /gene="folE"
                     /locus_tag="OQM52_001243"
     CDS             21311..21979
                     /gene="folE"
                     /locus_tag="OQM52_001243"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1563168.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21996..23072
                     /locus_tag="OQM52_001244"
     CDS             21996..23072
                     /locus_tag="OQM52_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1563169.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23214..24254
                     /gene="galS"
                     /locus_tag="OQM52_001245"
     CDS             23214..24254
                     /gene="galS"
                     /locus_tag="OQM52_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCS1563170.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24534..25532
                     /gene="mglB"
                     /locus_tag="OQM52_001246"
     CDS             24534..25532
                     /gene="mglB"
                     /locus_tag="OQM52_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1563171.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25593..27113
                     /gene="mglA"
                     /locus_tag="OQM52_001247"
     CDS             25593..27113
                     /gene="mglA"
                     /locus_tag="OQM52_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1563172.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27129..28139
                     /gene="mglC"
                     /locus_tag="OQM52_001248"
     CDS             27129..28139
                     /gene="mglC"
                     /locus_tag="OQM52_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1563173.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28175..29388)
                     /gene="preA"
                     /locus_tag="OQM52_001249"
                     /pseudo
     CDS             complement(28175..29388)
                     /gene="preA"
                     /locus_tag="OQM52_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29382..30620)
                     /gene="preT"
                     /locus_tag="OQM52_001250"
     CDS             complement(29382..30620)
                     /gene="preT"
                     /locus_tag="OQM52_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCS1563174.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30814..31053)
                     /locus_tag="OQM52_001251"
     CDS             complement(30814..31053)
                     /locus_tag="OQM52_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1563175.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31056..31775)
                     /gene="sanA"
                     /locus_tag="OQM52_001252"
     CDS             complement(31056..31775)
                     /gene="sanA"
                     /locus_tag="OQM52_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1563176.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31925..32809)
                     /gene="cdd"
                     /locus_tag="OQM52_001253"
     CDS             complement(31925..32809)
                     /gene="cdd"
                     /locus_tag="OQM52_001253"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1563177.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32939..33634)
                     /locus_tag="OQM52_001254"
     CDS             complement(32939..33634)
                     /locus_tag="OQM52_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1563178.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33631..34029)
                     /locus_tag="OQM52_001255"
     CDS             complement(33631..34029)
                     /locus_tag="OQM52_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1563179.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34268..35215
                     /gene="dusC"
                     /locus_tag="OQM52_001256"
     CDS             34268..35215
                     /gene="dusC"
                     /locus_tag="OQM52_001256"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1563180.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35586..35669)
                     /gene="yohP"
                     /locus_tag="OQM52_001257"
     CDS             complement(35586..35669)
                     /gene="yohP"
                     /locus_tag="OQM52_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1563181.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35893..37329
                     /gene="mdtQ"
                     /locus_tag="OQM52_001258"
                     /pseudo
     CDS             35893..37329
                     /gene="mdtQ"
                     /locus_tag="OQM52_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37382..38143
                     /locus_tag="OQM52_001259"
                     /pseudo
     CDS             37382..38143
                     /locus_tag="OQM52_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38260..38838)
                     /locus_tag="OQM52_001260"
     CDS             complement(38260..38838)
                     /locus_tag="OQM52_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1563182.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39008..39595
                     /locus_tag="OQM52_001261"
     CDS             39008..39595
                     /locus_tag="OQM52_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1563183.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39769..40701
                     /gene="pbpG"
                     /locus_tag="OQM52_001262"
     CDS             39769..40701
                     /gene="pbpG"
                     /locus_tag="OQM52_001262"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1563184.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40739..42454)
                     /gene="dld"
                     /locus_tag="OQM52_001263"
     CDS             complement(40739..42454)
                     /gene="dld"
                     /locus_tag="OQM52_001263"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1563185.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42650..44947
                     /gene="bglX"
                     /locus_tag="OQM52_001264"
     CDS             42650..44947
                     /gene="bglX"
                     /locus_tag="OQM52_001264"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1563186.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45199..46116
                     /gene="osmF"
                     /locus_tag="OQM52_001265"
     CDS             45199..46116
                     /gene="osmF"
                     /locus_tag="OQM52_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1563187.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46123..47280
                     /gene="yehY"
                     /locus_tag="OQM52_001266"
     CDS             46123..47280
                     /gene="yehY"
                     /locus_tag="OQM52_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1563188.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47273..48199
                     /gene="yehX"
                     /locus_tag="OQM52_001267"
     CDS             47273..48199
                     /gene="yehX"
                     /locus_tag="OQM52_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1563189.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48204..48935
                     /gene="yehW"
                     /locus_tag="OQM52_001268"
     CDS             48204..48935
                     /gene="yehW"
                     /locus_tag="OQM52_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1563190.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48916..49023)
                     /locus_tag="OQM52_001269"
     CDS             complement(48916..49023)
                     /locus_tag="OQM52_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1563191.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49083..49535)
                     /locus_tag="OQM52_001270"
                     /pseudo
     CDS             complement(49083..49535)
                     /locus_tag="OQM52_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11959 a        12205 c        13311 g        12060 t
ORIGIN      
        1 aaacagagtg gcgataatca tcaccatcgc aaaccagaac acagcgtgat aattccagat
       61 ctcggcgacg atgcccgcca ctgatcctgc gatgatccag cccacgcgcg acgtgttggt
      121 atagagcgtg gtggctgaac ctgcctgacc gggcatcaaa tcctgaaaat agagcatccc
      181 gatgccgccc agaatgccaa taaaaatagc atttagcagc tgcaagccca acagaatggc
      241 aggtgaatgc gccatcagca ttcctgcgta aaaacagacg ccacccacgg cagcaacgcg
      301 cattaagaaa cgcttaccca gacgtttggc gaaatatccg gcaatcaaca tcgtcgggat
      361 ttccagcccg gcggcggtcc ccatcatcac accggccagt ttctcgggaa gatgcagttc
      421 gttgataata aatagcggca tgttgatgat gtacaggctg ttcgagcccc acatcaatgt
      481 acaaatgaca aacagcagta gcgtatcgcg acggttacgg cgcggcgctt caaccgtgcc
      541 ggtcgccagc ggaagctctt tttgcatcga cggtaaaaac agccacacca tcacgccgca
      601 aacaataaac gctaccgctg cgctcagata cattaccgta aagctgaaac ccatcgctaa
      661 cgcataagcc agcggtgggc caatgaccca tgccagtgaa acctgagcgc gtaaaaaaga
      721 gctgaacatc accgcctcac gtccggtttt gtcggcatgt tcacgggcaa gggcaaacat
      781 ttgcgggtta gcggtcgagc caaagctgct aagaaagacg ccaacgaata gcaaaacaaa
      841 gtagttgcga ttccaagcaa aaagggtgca ggccagcacg cctaacaggc agcaaaagac
      901 aatcagcgat ttgcgatcgc cgcgcttatc agagcgccca gcgagaaact gacttaccag
      961 aatcccaatg acagcgctgc cggtaaagaa gaatcccacc atcgccggac gggcatgtac
     1021 ttcatcggta aggaaaatac tgagtgtcgg ggtttgcaga gcacccgcaa taccggtgag
     1081 aaaggcaacg attaaaaacg ccgtcgaggt caggtcaaac gatttcgcgc tggagactgc
     1141 gggggagtta tgcatgctgg tcgatcactg gaagaggaag acgcggagtt tacgctgctt
     1201 gtcagaaaat aaacagacag cgaaatcaaa agcgccacga ataatcaaaa tgccatttca
     1261 ctgttgctga ttggctgctg acgctgctga aatgaaattg ctgataacgg attttcccat
     1321 cagcaattag gaaaaatgac aaaaaattgt gcagcacatc aaacttttgc tcataacttt
     1381 acggctttcc ttgcgtgctg aaacagaagt attatgcttt cttgaaacgt ttcagcgcga
     1441 tcttgtcttt aaccctaagc taggttggcg ctttttttct catagaggct gaatcgtttc
     1501 aattcagcaa gagaggagaa ctatgttcca gttatccgta caggacatcc atccgggcga
     1561 aaaggccgga gacaaagaag aggcgattcg ccaggtcgct gcggcgctgg tgcaggccgg
     1621 taatgtagca gaaggctacg tcaatggcat gctggcgcgc gaacagcaaa cctcaacgtt
     1681 cctcggcaat ggtattgcta ttccacacgg cactaccgac acccgcgatc aggtgctgaa
     1741 aaccggcgtt caggtatttc agttcccgga aggcgtcacc tggggtgacg gtcaggtagc
     1801 gtacgtggca atcggtattg ctgccagctc ggataaacat ctgggcctgc tacgccagct
     1861 gacccacgta ctgagcgatg attccgttgc tgaacaactg aagtcagcaa caacagcaga
     1921 agaacttcgc gcattactga tgggcgaaaa gcagagtgag cagctgaagc tcgacaacga
     1981 aatgctgacg ctggatatcg tcgccagcga tctgctgact cttcaggcgc tgaacgctgc
     2041 gcgtctgaaa gaggcgaggg cagttgacgc cactttcgtc accaaagcca tcaatgaaca
     2101 accgctgaat ctcggacagg gtatctggct gagcgatagc gccgaaggca atctgcgtag
     2161 tgcgattgcg gtaagccgtg cggcaaatgc ttttgatgtg gacggcgaaa cggcagccat
     2221 gctggtgagt gtggcgatga atgacgatca gcccatcgcg gttcttaagc gtctcgctga
     2281 tttgttgctc gacaataaag ctgaccgctt gctgaaagcg gatgcggcaa cgttgctggc
     2341 gctgctgacc agcgatgatg cgccgaccga cgacgtgtta agcgcggagt ttgtggtgcg
     2401 caatgaacac ggcctgcatg ctcgtccagg taccatgctg gtcaatacca ttaaacaatt
     2461 taacagtgat attaccgtga caaaccttga tggcaccggc aaaccggcaa acggacgtag
     2521 tctgatgaaa gttgtggcac ttggcgttaa gaaaggtcat cgcctacgct ttaccgccca
     2581 gggtgcagat gctgaacagg cgctgaaagc cattggcgac gctatcgctg ctggtcttgg
     2641 ggagggcgca taatgagcag acgtgttgct acgatcaccc ttaatccggc ttatgacctt
     2701 gttggtttct gcccggaaat tgaacgcggc gaagtgaacc tggtgaaaac caccggtctg
     2761 catgcggcgg gtaaaggcat caacgtggcc aaagtattaa aagacctggg aattgatgtc
     2821 accgttggcg gcttcctggg taaagacaat caggatggtt ttcagcaact gttcagcgag
     2881 ctgggcattg ccaaccgttt ccaggttgta caggggcgca cccgaattaa cgttaagctg
     2941 acggaaaaag acggcgaagt gaccgacttc aacttctcgg gtttcgaagt cacccccgcc
     3001 gactgggaac gctttgtgac tgattctctg agctggctcg gtcagttcga tatggtctgt
     3061 gtcagcggaa gcttaccgtc aggcgtcagc ccggaagcgt tcaccgactg gatgactcgc
     3121 ctgcgtagtc agtgtccttg cattatcttt gatagtagcc gtgaagcgtt agtagcaggt
     3181 ttgaaagcgg caccgtggct ggtgaaacct aaccgccgcg agttggaaat ctgggcaggc
     3241 cgtaaactgc ctgaaatgaa agatgtgatt gaagctgcac atgcgctacg tgaacaaggt
     3301 attgcgcatg ttgttatttc actgggtgcc gaaggcgcgc tttgggttaa tgcctccggc
     3361 gaatggatcg ctaaaccacc gtcagtcgat gtcgtaagca ccgttggcgc aggggattct
     3421 atggttggtg gcctgattta tggcttgctg atgcgtgaat ccagtgaaca cacactgcgt
     3481 ctggcgacag ctgttgcagc cctggcggta agtcaaagca atgtgggtat taccgatcgt
     3541 ccgcagttgg ccgcaatgat ggcgcgcgtc gacttacaac cttttaactg acagcaggag
     3601 aggcataatg aaaacgctgc tgattattga cgctaatctc ggtcaggcac gcgcctatat
     3661 ggcgaagacc ctgctgggcg cggcggcgcg aaaagcaaaa ctggaaatca tcgacaatcc
     3721 gaacgacgca gaaatggcga ttgttctcgg tgattccatc ccgaatgaca gcgcgctgaa
     3781 cggtaaaaat gtctggctgg gtgatatttc ccgggcagtt gcgcaccctg agctgttcct
     3841 gagtgaagcc aaaggccatg cgaaacctta cactgcgccg gtcactgcga cagcaccggt
     3901 tgccgccagc ggtccgaaac gcgtagttgc ggtgactgct tgcccgactg gcgtagcaca
     3961 cacctttatg gcggctgaag ccattgaaac cgaagcgaaa aaacgtggct ggtgggtgaa
     4021 agttgaaacc cgtggttctg ttggcgcggg taatgcaatc actcccgagg aagtagccgc
     4081 agcggatctg gtgattgtgg cggcagatat cgaagtggat ctggcgaaat ttgctggtaa
     4141 accgatgtat cgtacctcta ccggtctggc gctgaagaaa acctcgcagg aactggataa
     4201 agcggttgct gaagcaacgc cgtatgaacc ggcgggcaaa gctcaaacgg cgaccactga
     4261 aggtaagaaa gagagtgcag gcgcttatcg tcacttgcta acgggcgtct cttacatgct
     4321 gccgatggtc gttgctggtg gtctgtgtat cgcgctttct tttgcttttg gtatcgaagc
     4381 gtttaaagag ccgggtacgt tggcagcggc gctgatgcag attggtggtg gttcagcctt
     4441 tgcgctgatg gtgccggtac tggcaggtta tattgccttc tccattgccg atcgtccggg
     4501 cctcacgccg ggtctgattg gcggtatgct ggcggtcagc accggttctg gcttcattgg
     4561 cggtattatt gcgggcttcc tggctggtta cattgcgaag ttaatcagta cgcaactgaa
     4621 actgccgcag agtatggagg cgctgaaacc gatcctgatc attccgctaa tttccagtct
     4681 ggtggtcggt ctggcgatga tctacctgat cggtaaacca gttgctggca ttctcgaagg
     4741 gctgactcac tggctgcaga ccatggggac tgcgaatgcg gttctgctgg gggcgatcct
     4801 cggtggcatg atgtgtactg acatgggcgg tccggtaaac aaagcagcgt acgcattcgg
     4861 tgtgggtctg ctgagtactc aaacctatgg cccgatggcg gcgattatgg cggcaggtat
     4921 ggtgccaccg ctggcaatgg gcctggcaac aatggtggcg cgtcgcaaat tcgacaaagc
     4981 gcagcaggaa ggtggcaaag ccgctctggt attgggactg tgcttcattt cggaaggtgc
     5041 aattccgttt gctgctcgtg atccgatgcg tgtgctgccg tgctgtatcg tgggtggggc
     5101 gctgactggc gcaatctcaa tggcgattgg tgcgaaactg atggcaccgc acggtggtct
     5161 gtttgttctg ctgatccctg gcgctattac gccggtattg ggttacctgg tagcaattat
     5221 tgccggtacg ctggtggcgg gtttggccta tgccttcctg aaacgtccgg aagtggacgc
     5281 agtagcgaaa gcagcgtaat aaaaggtgtg ttacagggca gaaatttcct gccctgtaac
     5341 atttgatgag cgcaaagaga atgaccagct tgtgaatgta attttatgaa tttaaatggt
     5401 tttatctttc agtagaaact tcaaacgttc atcaaacact aaggttattt cctcaagggc
     5461 agattatttt ctgccctttt ttagtgttcc tgttctgtgc atcccgtcac aaattcctcg
     5521 cttttgtaat tcaggcattt aatcgcattt tgtgagcctg ctcaaatctg cccattttgc
     5581 ctctctggcg gtaatttact ttacgtaaaa catgcatact atgagcacat gtttaaatat
     5641 gaacacatgt ttaaaccgtc tttgcagtat ctatattctt gtggattgac agggttggta
     5701 attttttacc agaggacgtg ctatgcgcga aaaggattat gtcgtaatta taggttcggc
     5761 gaatattgat gtcgccggat attcacatga atcattaaat tatgcggatt caaatccagg
     5821 taaaataaaa tttacgcctg gtggagtagg gcgcaatatt gcacaaaacc tggcgttgct
     5881 gggtaacaaa gcctggctac tgagcgccgt aggcagtgat ttttatggtc aatcgctgct
     5941 aacgcaaacc aatcaatctg gtgtttatgt cgataaatgc ctgattgtgc cgggagaaaa
     6001 tacgtcgagt tatttatcat tactcgataa taccggtgaa atgctggttg ctataaatga
     6061 catgaatatt agcaacgcta ttacagctga atatctcgca cagcaccgtg aatttattca
     6121 gagggcaaag gtcattgtcg cggactgtaa tatcagtgaa gaggcactgg catggattct
     6181 ggataatgcc gccaacgtac ccgtatttgt cgatccggtt tcagcgtgga aatgtgtcaa
     6241 agtgcgcgac cgtctaaatc agaaccacac tctgaagcca aaccgccttg aagcggaaac
     6301 cctgagtggg attgcgctgt cagggcgtga cgatgtggca aaagttgctg cctggttcca
     6361 tcaacatggc ctgaaccgac tggtattgag catgggcggc gacggcgttt attacagcga
     6421 tatcagcggt gaaaatggct ggtctgcgcc gatcaaaacc aatgttatta atgttaccgg
     6481 agcgggcgat gccatgatgg cgggacttgc ttcgtgttgg gtagacggaa tgccgtttgc
     6541 cgaatctgtt cgtttcgcac agggatgttc gtcaatggcg ctctcctgtg aatacaccaa
     6601 taaccccgat ttatcgattg ccaacgttat atcgttagtg gagaacgcag aatgtctgaa
     6661 ttaaaaattt cccctgaatt attacaaatt tctccggaag tgcaggacgc tttaaaaaac
     6721 aaaaaaccgg ttgtggcgct ggaatcgacc attatttctc acgggatgcc gttcccacaa
     6781 aatgcccaga ccgcaattga agtagaagaa actattcgta aacagggcgc agtacctgcc
     6841 actatcgcca ttattggcgg cgtgatgaaa gtgggtttaa gcaaagaaaa aattgaatta
     6901 ctgggtcgtg aagggcataa cgtgaccaaa gttagtcgtc gcgatttacc ttttgttgtt
     6961 gccgccggaa aaaatggcgc aaccactgtg gcttcaacga tgattattgc ggcgcttgcc
     7021 ggaattaaag tatttgccac cgggggaatt ggtggtgtgc atcgcggggc ggaacatacc
     7081 ttcgatattt ctgccgattt gcaagaactg gcaaatacta atgtcaccgt tgtttgtgcc
     7141 ggggcgaaat ctattctcga tttaggatta accactgagt atttagaaac cttcggtgtg
     7201 ccgttaattg gctatcagac taaagcgctg cctgcgtttt tctgccgtac cagctcgttt
     7261 gacgtcagca ttcgtctcga cagcgccagt gaaattgccc gtgcaatggc ggtgaaatgg
     7321 caaagcgggc tgaacggtgg cctcgtggta gcgaacccga tcccggaaca gtttgcgatg
     7381 ccggaggaat ctatcaatgc agccatagat caagccgtcg ccgaagccga agagcagggc
     7441 gttattggta aagaaagtac accgttcctg ctggcgcgcg ttgctgagct gaccagcggt
     7501 gacagcctga aatccaatat ccagctggtg ttcaacaacg ccattctggc gagtgaaatt
     7561 gccaaagaat accagcgtct cgcgggttaa tttctgacgg gcagaaagcc gtcgcccgta
     7621 ctcatcaagc ataccaaaac ctggcgctga cgccgggttt actggcatga agggaaaatc
     7681 attatggata taatgagaag tgttgtgggg atggtggtgt tactggcaat agcatttctg
     7741 ttgtcagtga ataaaaagag catcagtttg cgcacggttg gagccgcact gctgctgcaa
     7801 atcgctattg gtggcatcat gctctacttc ccaccgggaa aatgggcagt agaacaggcg
     7861 gcattaggcg ttcataaagt gatgtcttac agtgatgccg gtagcgcctt catttttggt
     7921 tcgctggttg ggccgaaaat ggatgtcctg tttgacggtg cgggttttat cttcgccttt
     7981 cgcgtacttc cggcgattat tttcgttact gcgctcatca gtctgctgta ctacattggc
     8041 gtgatggggc tgctgattcg catccttggc agcattttcc agaaagccct caacatcagc
     8101 aaaatcgaat cttttgttgc ggttaccact attttcctcg ggcaaaatga gatcccggcg
     8161 atcgttaaac cgtttatcga tcgcgtgaat cgcaacgagt tgtttaccgc aatttgtagc
     8221 gggatggcgt ccattgctgg ttcggtgatg attggttatg ccggaatggg cgtaccaatt
     8281 gactacctgt tagcggcatc gctgatggcg atccccggcg gtattttgtt tgcacgtatt
     8341 cttagcccgg caaccgagcc ttcgcaggtc acatttgaaa atctgtcgtt cagcgaaacg
     8401 ccgccaaaaa gctttatcga agcggcggcg agcggtgcga tgaccgggct gaaaatcgcc
     8461 gctggtgtag cgacggtggt aatggcgttt gtcgcaatta ttgcgctgat caacggcatt
     8521 atcggcggaa ttggcggctg gtttggtttc gccaatgcct ctctggaaag tatttttggc
     8581 tatgtgctgg caccgctggc gtggatcatg ggtgtggact ggagtgatgc caaccttgct
     8641 ggtagcctga ttgggcagaa actggcgatt aacgaattcg tcgcttacct gagtttctcc
     8701 ccatacctgc aaacgggcgg cacgctggaa gtgaaaaccg tggcgattat atcctttgcg
     8761 ctttgtggtt ttgctaactt tggttctatc ggtgttgtcg ttggcgcatt ttcggctatt
     8821 tcgccaaaac gtgctccaga aatcgcccag cttggtttac gggcgctggc agcagcaacg
     8881 ctttccaacc tgatgagtgc gactattgcc gggttcttta ttggtctggc gtaatgctcg
     8941 atttgtagac cggataaaac gtattaagcg ttgcatccgg caatcgcgct attgcaaata
     9001 atttttactg catcatcccg gagaatttat tctccgggtc catctccagc gccagtcctg
     9061 acaactgctt tctgttttta attagatatc ctttctttta attaataaac cgtcgtgaat
     9121 gaattgcgcg agaacatata aaagatgtcg ataagaaacg cctaaatact ctgccgcttg
     9181 cgtatgcttt tcgtgataaa gatcaccttc ttgtgagagt aatataaatg ctgccaggcg
     9241 attcactaac ggaaatgatt gattctgagt taaagaaaca atattacgat aatttttatg
     9301 acttaaggtg acgcaaagtt ttcgtaaaaa tagggtgtcg tttaataaca gcggacggta
     9361 atgtttcata gggagcgcaa ggcaccaaca ctcttcaata gcctgcaccg cacgcggttc
     9421 atggtctttg tcgattaact caatctcgcc aataaaacag ggagcggcaa agaaatcgat
     9481 cagcgacacg cgaccattgg caagcgtggc gtaaagcctg gcgcggcctc gcgtcagata
     9541 aaacagccat gacggctgtt gcccttcctg cacaatgtag tcacgcgcta aaaaatgaaa
     9601 cagccgcgta tcggctgaaa catccgttga aaagcaatcc ttaaacgcag attcactcat
     9661 caattgctgc ttaaggtcat tattgtggat ttctttcacg atgtttatcc gctatgagat
     9721 ttctcatatt tagcatacgt acgctgttgt taagattcaa tcattggccc ggtcgaattt
     9781 tctttgctgt agaaaaaaat aacgcaactg gaaacagagg aaataaacaa tggaaaagag
     9841 aaaaattatt ctggattgtg agccgggtca tgatgatgct attgcaatga tgatggcagc
     9901 gaaacatccg gcaatagatt tattaggcat cactattgta gcgggtaatc agacgcttga
     9961 taaaacatta attaatggcc tgaatgtttg ccagaaactg gagattaatg ttccggttta
    10021 tgcggggatg ccgcaaccca ttatgcgtaa acaaatcgtt gccgataata ttcacggtga
    10081 aaccggactg gatggcccgg tattcgagcc gctgacccgc caggcagaaa gtacccatgc
    10141 ggtgaaatat atcatcgata ccctgatggc aagcgatggc gatatcactc tggtgccggt
    10201 tggtccgctt tcaaatatcg cggtggcaat gcgtatgcaa cccgcaatcc tgcccaaaat
    10261 ccgtgaaatt gtgctaatgg gcggcgctta cggtacaggc aacttcaccc catctgccga
    10321 gttcaatatc tttgccgacc cggaagccgc ccgcgtggtg ttcacctccg gcgttccatt
    10381 agtgatgatg ggcctcgatc ttaccaacca gaccgtttgc accccggatg taattgctcg
    10441 gatggaaagg gtaggcggcc ctgccggaga gctgttcagc gacatcatga acttcactct
    10501 caaaacgcag ttcgaatact acggcctggc tggcggcccg gtgcatgacg ccacctgcat
    10561 tggttatctg atcaaccctg atggcattaa aacccaggat atgtacgtcg aagtggacgt
    10621 caacagcggc ccttgctacg ggcgtacggt ctgcgacgaa ctgggcgttc ttggcaagcc
    10681 cgctaatacc aaagtcggca tcactattga taccgactgg ttctggggct tagtcgaaga
    10741 gtgcgtgcgc ggctacatca aaacccatta agtgttgata acaagccggg acagtatcgc
    10801 aagatgcagg cccggctatt tctgatgggc agaaagccgt cgcccaaaat gacatgaaga
    10861 gataatcact atggatgtca tgagaagtgt tctgggaatg gtggtactgc tggcaattgc
    10921 gtttttactg tcagtaaaca agaagaagat cagcctgcgt accgttggcg cggcgttagt
    10981 gttacaggtc gtgattggcg ggattatgct ttggttaccg ccagggcgtt gggtcgcaga
    11041 aaaagtcgct tttggcgtcc acaaagtgat ggcgtacagc gacgcaggta gcgcatttat
    11101 cttcggttct ctggtcggac cgaaaatgga taccttattt gatggcgcag gatttatctt
    11161 tggtttcagg gtattaccgg caattatctt cgtcactgca ctggtgagta ttctctacta
    11221 catcggtgtg atggggatct taattcgcat tctcggcggt atcttccaga aagctttaaa
    11281 tatcagcaag atcgagtcat tcgtcgcggt caccaccatt ttcctcgggc aaaacgaaat
    11341 tccggcgatc gtcaaaccct ttatcgatcg tctgaatcgc aatgaattat ttacagcgat
    11401 ttgtagtggc atggcctcga ttgctggttc gacaatgatt ggttacgccg cactgggcgt
    11461 acctgtggaa tatttgctgg cggcatcgtt aatggcgatc cctggcggga tcttgtttgc
    11521 ccgcctgtta agcccggcta cggaatcttc gcaggtttct tttaataacc tctctttcac
    11581 cgaaacaccg ccaaaaagca ttattgaagc cgatgcgaca ggggcaatga ccgggctgaa
    11641 aatcgccgcc ggtgtagcga cagttgttat ggcatttgtc gccatcattg cgttaattaa
    11701 cggtattatc ggcggcgttg gcggctggtt tggttttgaa catgcctcgc tggagtccat
    11761 tttaggttac ctgctggccc cacttgcgtg ggtaatgggt gttgactgga gtgatgcaaa
    11821 tcttgccggg agtttgattg gacagaaact ggcaataaat gaatttgtcg cttatctcaa
    11881 tttctcaccc tatctgcaaa cggctggcac tctggatgct aaaaccgtgg cgattatttc
    11941 cttcgcgttg tgcggtttcg ctaactttgg ttctatcggg gtggtggtgg gggcgttttc
    12001 tgcggttgcg ccacaccgtg cgccggaaat cgcccagctt ggtttacgcg cgctggcggc
    12061 ggcgacactt tctaatctga tgagtgccac cattgccggg ttctttattg gtttagcgta
    12121 gtgtgcaatt tatgagccgg attagatgcg gtcaagcgtc gaatccggca acattgttac
    12181 accattggca ctaatgacag cgcgttatcg gcagacaggg tagggttgtt taagctgccg
    12241 ctggcgcgtg aaattgccgc gcaggccatc gcaaaacggg cgctgtcacg gaaattgttt
    12301 ccctccagaa agctatatac cagacccgcc ataaaaccat cgtcagcacc aaaactgtct
    12361 accgtcgtat gcgctggcac agtcagcaaa aattgttcac catccttttc gctgcaataa
    12421 actgactcat cgggcaaata aacaaacagt tgctgaacac cttgctgatg caatgcattc
    12481 actgcggcat tacggtcagc atcgctggtg atcgcctgtc cccataaaat ttccagctcc
    12541 ggtaaagtgg gtttcagggt gtgaatatgc gccagccagt gtttgatttt gcccgctttg
    12601 aattctgaaa cggtatcgac aaacaccggg atctcatcag cgagggtaaa gacccattcc
    12661 agcgcctcgg ctgtcaggtt gcaatcagcc agtaccacgc ccgcatgacg aagtaaatcg
    12721 cgcgacccgt tcagtagttg cggtgtcaac tgttccagca gatgggtatc gttaattgcc
    12781 agcacggttt gatcgtctcg attggcaatt gccagatacg tcgatgtgct ttgaccatgc
    12841 aaacgaacgc agccggagac attcacgccg gcacggcgcg tttcttccag gagcatttcg
    12901 ccataaaagt catcgccaat cactgaaagc aaatggacgt cacggcctaa cagcgccaga
    12961 ttgtgggcga tattgcgtcc aacgccgcct gccgaacaat gaattgtacc gggatgagaa
    13021 gccgcttgcg ggtaatggat atccgccatc ccgcgaatat ccatattgat tgtccccacc
    13081 actacgcagt attcctgctc ggtgagaatg taacctttgt ctttaatccg gcctttgcgc
    13141 attaaatcca taatatgcgc cgcaacgcgc gaacgactga tttgcagcat gtccgcaatt
    13201 tcgttctgct gaatcagcgg gttacgccgt aaaattgcaa ggatctcctt ttcccggtta
    13261 tttatcttca ggctaccgct ttttcagttt gttgcgcttt cagccaggcg atctcttctg
    13321 cccagatatc cgggttgatg gtttcaagga tcagcggaat gccgtcgaaa cggtcgttct
    13381 gcataatcca gcggaacgca tcatgaccga tattgccttc accgaggcta tgatggcggt
    13441 caacgcggct gccaaaggta cttttcgcat cgttaaggtg catcccgcgc aaatacttaa
    13501 agccgacaat acgggcaaaa tcagcgaacg ttttttcgca ttcggctgga gtacgcaaat
    13561 catacccggc agcgaaagca tggcaggtat caatgcagac gccgacgcgg gatttatctt
    13621 ccacgccgtc gataatcgcc gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac
    13681 cggcggtgtt ttcaatcacc gctgttacac cttgagtttt atccagcgca atgttgatgg
    13741 attcggcaat acgcgcaagg caatcctctt ctgaaatctg catcagatgg ctgccagggt
    13801 ggaagttgag caaagaaagc cccagttgtt cgcaacgctg catttcatct ataaaggcat
    13861 cgcgcgattt ttccagagct tcagtgaccg gatgtccgag gttgatcaga taactgtcgt
    13921 ggggaagaat ttgcgccgat gtgtagtgat atttttcaca ggcggctttg aattcatcga
    13981 tggtttgcgt cgtgagcggt gcggcacgcc actgacgttg gtttttggtg aacaaggcaa
    14041 acgcggttgc gtcgatttcg gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac
    14101 taacgtgcgc tccaatgtat ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg
    14161 gtaatgatag cgggttaaca ggagcaagaa gtagtggttt atgcgatgac gctttgaatc
    14221 acatagttaa tcgcaccacc accaacaatc agccaggcaa acagtaccag tgccatcagc
    14281 agaggtttcg ccccagcttt tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc
    14341 atcgccattg ccagcaggaa ggtatccagc gttaccagca tgttcaccac gctctgcggt
    14401 aacaggtgga acgagttaaa gatggcaact acgatgaaca agatggcaaa ccacggaata
    14461 gtgattttgc ttttctcgcc gctgttcgcc ccagacagct gtttaacacg cgccgccagc
    14521 aggatgagga acggagccag catcatcacg cgcagcattt tggaaataac tgctgcgttt
    14581 tctgcatccg ggctgatggc atgacctgcc gccaccacct gcgccacttc gtgcacagta
    14641 gaaccaatgt agataccgaa agtttccgga ctaaaccatt gagacatcag cggatatatc
    14701 gccgggtaga ggaaaatcgc gacggtcccg aagataacaa cggttgcaac agccacggtt
    14761 actttactgg cttccgcttt cactaccggc tcagtcgcca gtaccgcggc agcaccacag
    14821 atactgctac cggcaccgat caaccaactg gtgtgcttat ccagaccaaa cactttctgc
    14881 cccaggaagc aagccagcag gaaggtactg gacagcgtca gcacgtcaat gatgatccca
    14941 ctgataccga catcggcaat ttgcgagaac gtcagacgga agccataaag aatgataccc
    15001 agacgtaata aatattgctt ggcaaacagc acaccaccgt cacagctttt ccagatgtgc
    15061 ggatagatgg tgttgcctaa aaccatcccc aacaagattg cgagggtgag ggcactaaac
    15121 ccggcacccg caaccgcggg aatggaacca ccccacaggg cgaccccggt gataactgca
    15181 ctcagggcta accccggaat aaaatgccac agtgtacgat gttgtttctg taaggtgata
    15241 ttcgtcataa ccctctcctt taaccgggca taaggttacg actaactggt ttaaaaataa
    15301 aattgattat atatttataa ttaatcttta taagtggtaa gcgactatgc acatcaccct
    15361 ccggcagttg gaagtttttg cagaagtatt gaaaagtgga tcaaccaccc aggcgtcggt
    15421 gatgctggcg ttgtcgcaat cagcagtgag cgcagccttg actgacctgg aagggcagct
    15481 tggcgttcaa ctgtttgatc gcgtggggaa aagactggtt gttaatgaac acgggcggct
    15541 gctctatccg cgagcgttgg cactgcttga acaggcggtt gaaatcgaac aactgtttcg
    15601 cgaagacaac ggcgcgattc gtatctatgc cagtagtacc atcggtaact acattctgcc
    15661 tgcagttatc gcccgttatc gccatgatta tccgcagttg ccgattgaac ttagcgttgg
    15721 gaacagccag gacgtgatgc aagcggtgct ggatttccgc gttgatattg gctttattga
    15781 aggaccgtgc cacagcactg aaatcatttc tgaaccgtgg ctggaagacg agctggtggt
    15841 tttcgccgcg ccgacttcgc cgttggctcg tggtccggtc accttagaac agctggccgc
    15901 tgcgccgtgg atcctgcgtg aacgcggttc cggcacgcgg gatattgtcg attatctgtt
    15961 gctgtcacat ttaccgaagt ttgagatggc gatggaatta ggtaactccg aggcaatcaa
    16021 acatgcggtg cgtcatgggt tgggaattag ttgcctgtcg cgacgtgtga ttgaagatca
    16081 attgcaggca ggcacattaa gtgaagttgc ggtccctctg ccgcgcctga tgcgtacgtt
    16141 gtggcgtata catcatcggc aaaaacacct ttccaacgcg ctacggcgct ttctggacta
    16201 ttgcgatccc gcaaatgtgc cgcgttaagt tgctgtacaa gaacatgctg gtgctgtgtc
    16261 gatttcgtga cgcagcgcct tcagcatgca ttcgccagaa aagagattgg ctgctttact
    16321 tataatccct gggcgatcat gaaggtgtct tataaccgtg tatttctgcc ggaaggattg
    16381 ccaatcgtct gctacaatcg cgccatcatt tttaagatgg atagcatttt tgtatgggtt
    16441 ccgaaactaa aactacagaa gcgccgggct tacgccgtga attaaaggcg cgtcacctga
    16501 cgatgattgc cattggcggt tccatcggta caggtctttt tgttgcctct ggcgcaacga
    16561 tttctcaggc aggtccgggc ggggcattgc tctcgtatat gctgattggc ctgatggttt
    16621 acttcctgat gaccagtctc ggtgaattgg ctgcatatat gccggtttcc ggttcgtttg
    16681 ccacttacgg tcagaactat gttgaagaag gctttggctt cgcgctgggc tggaactact
    16741 ggtacaactg ggcggtgact attgccgttg acctggttgc agcacagctg gtcatgagct
    16801 ggtggttccc ggatacaccg ggctggatct ggagtgcgtt gttcctcggc gttatcttcc
    16861 tgttgaacta catctctgtt cgcggctttg gtgaagcgga atactggttc tcactgatca
    16921 aagtcacgac agttattgtc tttatcatcg ttggtgtgct gatgattatc ggtatcttca
    16981 aaggcgcgca gcctgcgggc tggagcaact ggacaatcgg cgaagcgccg tttgctggtg
    17041 gttttgcggc gatgatcggc gtagctatga ttgtcggctt ctctttccag ggaaccgagc
    17101 tgatcggtat tgctgcaggc gagtccgaag atccggcgaa aaacattcca cgcgcggtac
    17161 gtcaggtatt ctggcgaatc ctgttgttct atgtgttcgc gatcctgatt atcagcctga
    17221 ttattccgta taccgatccg agcctgctgc gtaacgatgt taaagacatc agcgtcagtc
    17281 cgttcaccct ggtgttccag cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag
    17341 ttattctgac ggcggtgctg tcagcaggta actccggtat gtatgcgtct actcgtatgc
    17401 tgtacaccct ggcgtgtgac ggtaaagcgc cgcgcatttt cgctaaattg tcgcgtggtg
    17461 gcgtgccgcg taatgcgctg tatgcgacga cggtgattgc cggtttgtgc ttcctgacct
    17521 ccatgtttgg caaccagacg gtatacctgt ggctgctgaa cacctccggg atgacgggtt
    17581 ttatcgcctg gctggggatt gccattagcc actatcgctt ccgtcgcggt tacgtattgc
    17641 agggacacga cattaacgat ctgccgtacc gttcaggttt cttcccactg gggccgatct
    17701 tcgcattcat tctgtgtctg attatcactt tgggccagaa ctacgaagcg ttcctgaaag
    17761 ataccattga ctggggcggc gtagcggcaa cgtatattgg tatcccgctg ttcctgatta
    17821 tttggttcgg ctacaagctg attaaaggaa ctcacttcgt acgctacagc gaaatgaagt
    17881 tcccgcagaa cgataagaaa taagtttcct cccttccttg ctaagccctc tcaaccgaga
    17941 gggctttttc aattccattt ccctgacaaa tcatgcggat ataaaattta acatttggat
    18001 tgataattgt tatcgtttgc attatcgtta cgacgcaatc aaaaaaggct gacaaatcag
    18061 aggctgttcc ggctttctgg gatgatcacc tgcatacaaa taagtccacc gcgatgctgc
    18121 cgtacgcaag gggacgtaaa gaagatgtga gcgataccca ttttattttc gtagttacct
    18181 catggagata tggaatgttt aggttgaacc ctttcgtacg ggtcgggctg tgtttgtccg
    18241 ctatttcttg tgcatggcct gtgttagcgg tcgatgatga tggcgaaacg atggttgtca
    18301 ctgcatcttc cgtggaacaa aatcttaaag atgcacccgc cagtatcagc gtcattaccc
    18361 aggaagacct gcagcgaaaa ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg
    18421 gcgtacaact gacgaacgaa ggggataacc gtaagggcgt tagtattcgt ggtctggaca
    18481 gcagctacac cctgattctt gtcgacggta aacgcgttaa ctcccgcaat gccgtcttcc
    18541 gccacaatga tttcgatctg aactggatcc cggtcgattc catcgaacgt attgaagtgg
    18601 ttcgtggccc gatgtcgtcg ctatacggct ccgatgcgct cggcggtgta gtgaatatca
    18661 tcaccaaaaa aatcggtcag aaatggtcgg gtaccgttac cgtcgatacc accattcagg
    18721 aacatcgcga tcgcggtgat acctataacg gtcagttctt taccagtgga ccattaattg
    18781 atggtgtgct gggaatgaaa gcttacggca gcctggcaaa acgtgaaaag gatgacccgc
    18841 aaaactcaac gaccaccgat accggagaaa cgccgcgtat tgaaggattc tccagccgcg
    18901 acggcaatgt cgaatttgcc tagacaccga atcaaaatca cgattttact gccggatacg
    18961 gtttcgaccg tcaggatcgt gattccgatt cgctggacaa aaaccgcctg gaacgccaga
    19021 actactgcgt cagccataat gggcgttggg attacggcac cagcgaactg aaatcctacg
    19081 gtgagaaagt cgagaacaaa aaccctggca acagcagccc gataacttcc gaaagcaata
    19141 cggtcgacgg caaatacacg ttgccgctga cggcgattaa tcagtttctc acggttggcg
    19201 gtgaatggcg tcacgacaaa cttagcgatg cggtgaacct gaccggggga accagctcca
    19261 aaacgtctgc cagccagtac gcgctgtttg tggaagatga atggcggatc ttcgagccgc
    19321 tggcgctgac gaccggcgtg cgtatggacg atcacgaaac ctacggtgaa cactggagtc
    19381 cgcgtgccta catggtttat aacgccaccg acaccgtaac ggtgaaaggg ggctgggcga
    19441 cggcatttaa agcaccttct ctgttgcaac ttagccctga ctggacgagc aattcctgcc
    19501 gtggcgcatg taagattgtg ggtagcccgg atctgaaacc agaaaccagc gaaagttggg
    19561 agctggggct ttactacatg ggtgaagaag gctggctgga agggggtgaa tccagcgtta
    19621 tcgttttccg taacgatgtg aaagatcgta tcagcatcag ccgtacgtct gacgtcaacg
    19681 ctgcaccggg ctaccaaaac tttgttggtt ttgagacggg cgctaacgga cggcgcatac
    19741 cggtatttag ctactacagc gttaacaaag ctcgtattca gggcgtggaa accgaactga
    19801 aaattccgtt caacgatgaa tggaaactgt cgatcaacta cacctacaac gatggtcgtg
    19861 atgtcagcaa cggcgaaaac aaaccgctat ccgatctgcc gttccatact gctaacggta
    19921 cgctggactg gaaaccgctg gcgctggaag actggtcatt ctatgtttct ggtcactata
    19981 ccgggcagaa acgcgccgac agcgcgacgg ctaaaacacc gggcggttat accatctgga
    20041 ataccggcgc ggcctggcag gtgactaaag acgtcaaact gcgcgcaggc gtgctgaacc
    20101 ttggcgacaa ggatctcagt cgtgacgact acagctataa cgaagacgga cgtcgttact
    20161 ttatggcagt ggattatcgc ttctgatgag aagatgcccg gcgaaccggg cggactttca
    20221 cttcagtaaa tactgcgcat ggaagcgcag gtgatcctct ataaaagagg cgatgaagta
    20281 gtaactgtga tcatatcccg gctgaatacg cagcgtcatc ggccacgctt tctggcgcgc
    20341 ggcttctgcc aataccgcag gttgcaactg gtcggcaaga aactgatcat tatcgccctg
    20401 atcgataagc gtcgggatcg catcctgcgc gttactggca tacatcagcg cgcaactatc
    20461 ccattccagc atgcattttt gtcctcacct aaataggtgc taaacgcttt gattccccac
    20521 gggacgctgc acggattcac aatgggcgca aaggccgaaa cgctggtgta tttacccgga
    20581 tttttcagcg ccataatcag cgcaccgtga ccacccattg agtgaccgct aatggcgcag
    20641 cggtcgctga cattaaattg cgactgaacc agcgccggta attcatcgcg cagataatca
    20701 tacatccggt aatgcgtcgc ccacggcggt tgcgtggcat taagataaaa gcctgcgccc
    20761 tggcctaaat cgtagccatc gtcgttggca accttttcgc cgcgcgggct ggtgtctggc
    20821 atcaccagta caatccccag ttccgccgct acccgctggg cacccgcctt ggtggtgaag
    20881 ttctcgtcat tgcaggttaa tccggaaagc cagtacagca ctggtggcgg ggtgtgatca
    20941 cgaggggggg ggagaaagat actgaacgtc atcgggcagt ttaaggtact ggagtcgtgt
    21001 cgccagcgtt gctgccagcc ttcaaaacag cggtgctctt cgagcatttc catgcgtggc
    21061 tccttgttgt gttgtttgca atttcttcat cataccgttt attcaatgtg ctgtgagtaa
    21121 ctttcacttc cgtatttgca taacgatgtt ttaacatctg ctgatgaaag gcagcggcaa
    21181 ttacaataat tatcgctgtg aatactggat tatgtgcgcc gcctcacgca caataatcaa
    21241 gctgtaaatc agcttaataa ctttgccccc acgcagggtg gaggcgtcac acctgcagga
    21301 gaaatcataa atgccatcac tcagtaaaga agcggccctg gttcatgaag cgttagttgc
    21361 gagaggactg gaaacaccgc tgcgcccgcc cgtgcatgaa atggataacg aaacgcgcaa
    21421 aagccttatt gctggtcata tgaccgaaat catgcagctg ctgaatctcg acctggctga
    21481 tgacagtttg atggaaacgc cgcatcgcat cgctaaaatg tatgtcgatg aaattttctc
    21541 cggtctggat tacgccaatt tcccgaaaat caccctcatt gaaaacaaaa tgaaggtcga
    21601 tgaaatggtc accgtgcgcg atatcactct gaccagtacc tgtgaacacc attttgttac
    21661 catcgatggc aaagcgacgg tggcttatat cccgaaagat tcggtgatcg gtctgtcaaa
    21721 aattaaccgt atcgtgcagt tctttgccca gcgtccgcag gtgcaggaac gtctgacgca
    21781 gcaaattctt attgcgctac aaacgctgct gggcaccaat aacgtggctg tctcgatcga
    21841 cgcggtgcat tactgcgtga aggcgcgtgg catccgcgat gcaaccagtg ccacgacaac
    21901 gacctctctt ggtggattgt tcaaatccag tcagaatacg cgccacgagt ttctgcgcgc
    21961 tgtgcgccat cacaactgat taaaaggcag gaaccatgga gcgcaacgtc acgctcgatt
    22021 ttgttcgcgg cgtcgccatt ctggggatcc tgctattaaa catcagcgcc tttgggctac
    22081 caaaggcggc ttatctcaat cccgcctggt acgatgctat tacgccgcag gatgcatgga
    22141 cctgggcatt tctcgatctc atcggccagg tgaaattcct cacgcttttt gcgctgctgt
    22201 ttggtgctgg cctgcaaatg ttgctgcccc gtggcagacg ctggatccag tcgcggttaa
    22261 cgctgttagt cttgttgggc tttattcacg gtttattgtt ctgggacggc gatattctgc
    22321 tggcttacgg gctggtgggg cttggcgttt tgctgttatt ggggctgatt tccgacagcc
    22381 agaccagccg cgcctggacg ccggatgcat cggctatttt gtatgaaaaa tactggaagc
    22441 ttcacggcgg cgttgaagcg atcagtaatc gtgccgatgg tgttggcaac agtttactgg
    22501 cactgggcgc acagtatggc tggcaactgg cagggatgat gctcattggt gcggcgctga
    22561 tgcgcagcgg ctggctgaaa gggcagttca gcttacgtca ctatcgtcgt actggttttg
    22621 tgctggtggc gattggggtg accattaacc ttcctgccat cgccctgcaa tggcagctgg
    22681 actgggcgta tcgctggtgt gccttcttac ttcaaatgcc gcgggaactg agtgcgccgt
    22741 ttcaggcgat tggctatgcg tcgctgtttt atggcttctg gccgcaattg agccgcttta
    22801 agctggtgct tgcgatcgcc tgcgtcggac ggatggcgct gaccaactat ctattgcaaa
    22861 cgctgatttg taccacgctt ttttaccacc tcggtttgtt tatgcatttt gaccgcctgg
    22921 agctgctggc gtttgttatt ccggtatggc tggcgaatat cctcttctct gttatctggc
    22981 tgcgttactt ccgccagggg ccggtggaat ggctctggcg tcagttaact ttgcgtgctg
    23041 ccggaccggc aatatctaaa acatcaagat aacgatctgg attacattcg ttaacaaaac
    23101 ggctgtaacc gtttccattg ctgtgactcg attcacgaag tcctgtattc agtgctgaca
    23161 aaatagccgc cagcaagcag tcatttactg caatctcata acaggtagtg aatatgatca
    23221 ccattcgtga tgtagcgcgt caggctggcg tctctgtggc aacggtttcc cgggtgctca
    23281 ataacagcac gctggtcagt gccgacacgc gtgaagcagt aatgaaagcc gtgagtgagc
    23341 tggattatcg gccaaacgcc aatgcccagg cgctggcaac tcaggttagc gacaccattg
    23401 gcgtggtggt gatggacgtt tctgatgcgt ttttcggcgc gctggtaaaa gcggtggatc
    23461 tggtcgccca gcagcatcag aaatacgtgc taatcggcaa tagctatcat gaagcggaaa
    23521 aagagcgtca cgccattgag gtgttaattc gccagcgttg taatgcgttg attgttcact
    23581 caaaagcatt gagtgacgat gaactggcgc aatttatgga taacattccc ggtatggtgt
    23641 taatcaaccg cgttgtgccg gggtacgccc atcgttgcgt ttgcctggat aatctcagcg
    23701 gtgcccgaat ggcgacgcgc atgttgctga ataacggtca tcaacgtatt ggttatcttt
    23761 cttccagcca cggcattgaa gatgacgcca tgcgtaaagc aggctggatg agtgcgttga
    23821 aagagcagga tattattccg ccggaaagct ggattggcac tggtacgccg gacatgccgg
    23881 gcggtgaggc ggcgatggtt gaactgctgg ggcgcaatct acaacttacc gctgtatttg
    23941 cttataacga caatatggcc gctggcgcac tgacagcatt aaaagataat ggcattgcga
    24001 ttccgttaca tctctcaatc atcggtttcg atgatattcc catcgcccgt tacaccgacc
    24061 cgcaattaac gaccgtgcgt tatcccattg cttcaatggc taaattagcc accgaactgg
    24121 ccttgcaggg ggcagcaggc aatattgatc ctcgtgccag ccactgtttt atgccgacgt
    24181 tagtgcgtcg ccattctgtc gcaacgcgcc agaatgcggc ggcgatcact aactcaacaa
    24241 atcaggcgat gtaaccgctt tcaatctgtg agtgatttca cagtatctta acaatgtgat
    24301 agctatgatt gcaccgtttt aatgttgtaa cccgtatgta acagtgaata atcactttca
    24361 ccgaggtaat agcgtcataa caacaattaa agccgttttc tggagcgtta ccgggcatgg
    24421 aagaacgaat tttaaaaagt gagcttcggc gttcagtaac acttcattaa ctctactgcc
    24481 ccgccgagca tttatctcaa gcactaccct gcataagaaa aaccggagat accatgaata
    24541 agaaggtgtt aaccctgtct gctgtgatgg ccagcatgtt attcggtgcc gctgcacacg
    24601 ctgctgatac tcgcattggt gtaacaatct ataagtacga cgataacttt atgtctgtag
    24661 tgcgcaaggc tattgagcaa gatgcgaaag ccgcgccaga tgttcagctg ctgatgaatg
    24721 attctcagaa tgaccagtcc aagcagaacg atcagatcga cgtattgctg gcgaaagggg
    24781 tgaaggcact ggcaatcaac ctggttgacc cggcagctgc gggtacggtg attgagaaag
    24841 cgcgtgggca aaacgtgccg gtggttttct tcaacaaaga accgtctcgt aaggcgctgg
    24901 atagctacga caaagcctac tacgttggca ctgactccaa agagtccggc attattcagg
    24961 gcgatttgat tgctaaacac tgggcggcga atcagggttg ggatctgaac aaagacggtc
    25021 agattcagtt cgtactgctg aaaggtgaac cgggccatcc ggatgcagaa gcacgtacca
    25081 cttacgtgat taaagaattg aacgataaag gcatcaaaac tgaacagtta cagttagata
    25141 ccgcaatgtg ggacaccgct caggcgaaag ataagatgga cgcctggctg tctggcccga
    25201 acgccaacaa aatcgaagtg gttatcgcca acaacgatgc gatggcaatg ggcgcggtag
    25261 aagcactgaa agcacacaac aagtccagca ttccggtgtt tggcgtcgat gcgctgccag
    25321 aagcgctggc gctggtgaaa tccggtgcac tggcgggcac agtactgaac gatgctaaca
    25381 accaggcgaa agcgaccttt gatctggcga aaaacctggc cgatggcaaa ggtgcggctg
    25441 atggcaccaa ctggaaaatc gacaacaaag tggtccgcgt accttatgtt ggcgtagata
    25501 aagacaactt ggctgaattc agcaagaaat aagactgatt gggcgcagtc gtttactgcg
    25561 cccttattgt atggccaaca aggtaaaatt atatggtcag ctcaacgact ccgtcttccg
    25621 gggaatactt gttggaaatg agcggtatca acaagtcctt tcctggtgtt aaggcacttg
    25681 ataacgttaa tttaaaagtc cggccacatt ctatccatgc attaatgggg gaaaacggtg
    25741 caggaaaatc gacattatta aaatgcctgt ttggtattta tcaaaaagac tccggcacca
    25801 ttttattcca gggtaaagag atcgatttcc attccgccaa agaggcactg gaaaatggta
    25861 tttcgatggt acaccaggag ttaaacctgg tattacaacg ttcggtgatg gataacatgt
    25921 ggctggggcg atatcccacc aaaggcatgt ttgtcgatca ggacaaaatg taccgcgaaa
    25981 ccaaagcgat ttttgatgaa ctggatattg atatcgatcc gcgtgcgcgc gtcggcacat
    26041 tatccgtttc gcaaatgcag atgatcgaaa tcgccaaagc gttttcctat aacgcgaaaa
    26101 ttgtgattat ggatgaaccg acttcttcgt taaccgaaaa agaggtcaat catctgttca
    26161 ctattattcg taaattaaaa gagcgcggct gcggtattgt ttatatctcg cataaaatgg
    26221 aagaaatctt ccagttatgt gatgaagtta ccgtattgcg cgacggtcag tggatcgcca
    26281 ccgaaccgct ggcaggactg acgatggaca agatcatcgc catgatggtt gggcgttctc
    26341 ttaatcagcg ttttcctgac aaagaaaaca agccgggcga agtcatcctc gaggtgcgta
    26401 acctaacgtc actgcgccag ccgtcgattc gcgatgtctc gtttgatctg cataaagggg
    26461 agatcctcgg tattgccggg ctggtggggg cgaaacgtac cgatattgtt gagacgttat
    26521 ttggtattcg cgagaaatcg gctggcacca ttacgttgca cggcaaacag atcaataacc
    26581 ataatgccaa cgaagccata aaccacggat ttgcactggt aactgaggag cgccgctcaa
    26641 cgggaattta tgcctatctg gatattggtt ttaactcgtt aatttccaat attcgcaact
    26701 acaaaaataa agttggttta ctggataact cgcggatgaa aagcgatacc cagtgggtga
    26761 ttgattcgat gcgggtaaaa acgccgggtc atcggacgca aattggttcg ctctccggtg
    26821 gtaatcagca aaaggtgatt attggtcgct ggctactaac gcaaccagaa atattaatgc
    26881 tcgatgaacc gacgcgcggt attgatgtcg gggcgaagtt tgaaatttat cagttaattg
    26941 ccgaactggc gaagaaaggc aaggggatta ttattatctc ctctgaaatg cctgagttgt
    27001 tagggataac agaccgtatt ctggtcatga gcaatggtct cgtttccgga attgtcgata
    27061 caaaaacaac aacgcaaaac gaaattctgc gtcttgcgtc tttgcacctt taagattagg
    27121 ggcttcccat gagtgcgtta aataagaaaa gttttcttac ttacctgaaa gagggcggta
    27181 tttacgtcgt tcttttagtt ttgctggcga ttattatttt ccaggaccca acatttttaa
    27241 gtctgttgaa cttaagtaat attctcaccc agtcatcggt gcgtattatt atcgcgctcg
    27301 gtgtggcagg gttaattgtc acccagggga ccgatctttc tgctggtcgt caggtagggc
    27361 tggcggcagt ggtggctgcg acattattgc agtccatgga taacgccaac aaagtgttcc
    27421 cggaaatggc gacgatgccg attgcgctgg ttattctgat tgtctgtgcc attggtgcgg
    27481 tgatcggttt gatcaacggt ctgattatcg cttatctcaa cgtgacgccg ttcattacca
    27541 cgctcggcac gatgatcatc gtctatggca tcaactcgct ctattacgac tttgtcgggg
    27601 cgtcgccaat ttctggtttt gacagtggct tctctacctt tgctcagggc tttgtcgcgc
    27661 tggggagttt ccgtctctct tacatcacct tctacgcgtt gattgcggtg gcgttcgtct
    27721 gggtgttgtg gaacaaaacc cgcttcggta agaacatttt tgccattggc ggtaacccgg
    27781 aagcggcaaa aatatctggt gtcaacgtcg gcctgaacct gctgatgatc tacgcgttgt
    27841 ctggcgtgtt ctatgccttt ggcgggatgt tagaagccgg acgtatcggc tctgccacca
    27901 acaacctcgg ctttatgtat gagctggatg ctatcgcggc gtgcgtggta ggcggtgtat
    27961 cgttcagcgg cggtgtgggg acggtgattg gcgtggtgac cggggtaatt atttttaccg
    28021 tcatcaacta tggcctgacg tatatcggcg taaacccata ctggcagtac atcatcaaag
    28081 gggcgattat tatcttcgcc gtagcgctgg attcactgaa atacgcgcgt aagaagtgat
    28141 gtgattgcgg atattgccgg atgcgtctct ggttttacaa cgttaccggg tgttctttct
    28201 cgcctttctt aaacttcact tccccgagct caatacaacc caccgggcag acgtgaccac
    28261 aaagcagaca acccacacat ttctcggtat tacaatgcgg tgtgcgggtt ttctcgctcc
    28321 attccatcgc ctggtgaccg ccgtcgtaac aggaaatata acagcgtcca cagccaacac
    28381 atttgtcaag attgatacgg ggatagacaa tataactgcg gtctaaatct tccgccggga
    28441 caatattgtt attcgccaga cctaccatct cctgcaacga atcaaatccc tgatcggcga
    28501 gataatgcga taacccgctc gccatatctt ctacgatgcg atagccgtac tgcataatgc
    28561 cggtggtgac ctgtaacgtt gctgcgccga gcaataaaaa ctccgccgca tcttcccagg
    28621 tttcaatgcc gccgataccg ctgattggga aatcgcgcag ttctggatgg gtgcgcattt
    28681 gctgaatgaa gcgcagggcg atcggtttta ccgctttacc ggaatatccg gaaatactcg
    28741 atttaccgtt aacgatcggc ataccgattt tctgattaag atcgatattg gtgatggatt
    28801 taacggtgtt aattgccgca atgccatctg cgccgccgcg cttcgccgcc agcgccactt
    28861 cgcacatatc accgatattc ggcgtcatct tcgccagcat tggcagcgtg gaaccccgtt
    28921 tcactgcccg acaatatttt tctaccagct ccgggctttg cccgacatcg ctacccatcg
    28981 catgagaagt catttgcgga caggagaagt tacactcgat catatccgcg ccagctgctc
    29041 ccattgctgc tcattttccc ccatgatcga agcgatcaat actttgtccg ggtaatcttc
    29101 cttcagccga cgcagggcgg tcagattctc ttccaacgga tgttcagcaa tctgctccat
    29161 atttttgaag ccgataaaac cggtatcttc tttcaccaga tgatcaaaac gcggcgagac
    29221 ttcgttggcg ataaaaaagc cgatcgtttt aaacaccaca ccgccccaac ctgtgtcgta
    29281 ggctttggca cacatctcat agcagttgcc taccggcgaa gaagagaggc agaacgggtt
    29341 gggaaagttc acgccgcaaa aagtaatcga aagatctttc gttaacatga gcaagctccc
    29401 tctaaatagt gatgaatcgc ctcggcggct tctttcccgg ttttcacggc atagaccacg
    29461 gttttgtcac cctcaacaat atcgccagca gcaaagactt gcggatcgcg gatctgataa
    29521 ttttgtgttt tgatggtgtt acgctgctgc tctaactccg caaaggcatc cagtctggca
    29581 tgctgaccga cggcgagaat aattttatct gccgccatcg tcagttcgcc tgataaccgt
    29641 acatgcttaa acgtgacttt attgccttcc acggctactg gcgtgaatcc atcgatgatc
    29701 gaaacgccca gctcccgggc gctggcaaac tctttttcgc ttgccggaaa ctcatctaac
    29761 tcttcacgtg ctacgcaagt taccgcctga cagccgagaa ctttcagcgt gctggctacg
    29821 tccatcgcga catcaccgcc gccgataatt aatgcgcttt gtggaatgct gatatcgcct
    29881 tgcgcctgtc gtacacgttg caggaagtcg acggcaatct caacgtcgct atgctcaaac
    29941 agcggtagcc cggaaccgct tgataacccc acggtgacca gtaccgcgcg gttttccgct
    30001 ttcagttgct ccagggtgag tgttttaccg atttcgttat tgcacttaat ggtcacgccc
    30061 attttttcaa tacgggcgat ctctgcatcc agcactgatt gcggtaaccg gaattgcgga
    30121 ataccgttac gcaaccagcc accggggtgc gcttctttct cataaatcgt gacgtcataa
    30181 ccctggtttg tcagtgtcac actggcctgc aatccggcag gacctgcgcc aataatcgcg
    30241 actttgccga gcgttttagt accgggctga taaatttcca tcccggtttg ttgttcaaaa
    30301 tcagtaacaa aacgctgtaa gcggccgata tcaatgggag catcaacacc ggcacgggta
    30361 caaccgcttt ggcataattt ctccgtcggg cacactctgg cacaaacggc accgagggca
    30421 ttattttcgc gaattgtctc ggcagcgcct ttaaaattac gaaagtagat tgagcgaata
    30481 aatttccccg gatcggtctg tgccgggcaa gcctgactac agggagcgtc gtgacataat
    30541 aaacagcgag aggcttcttt aatcgccagt aacggcgtaa aagccggagt gagttcatcc
    30601 agataatttt gttgcggcat gtgatcttcc ttaatgctat gagtaattat ggcgaacgct
    30661 aaataaatat tgcgtgctga tattaaggct atgcgcgtgg ggaaatttaa gctgtgaaag
    30721 gattcacaaa tagctgcggt tcagaatgtt atatccgcag cttattgagt gaagtaataa
    30781 ttattctcaa taattaacag aaaagagaaa atattaacga tagaaaataa gataaacaat
    30841 catgccgata gataaaattc cgaagccaat atatgccacc atataagcga agaagagtcc
    30901 gggatttttt gctcgccaga catacaccgg ttccggtgca tttttaaccc gtttatcaat
    30961 cgcgcctgcg cgccagcctt tccatgcttc gcggaaacaa aatatcggga taaggaaaaa
    31021 aacggcaacg acaaaaaact gctgtacatc cataactact ttccttgttt cttttgtaat
    31081 tcaagtaact gttcgggtgt aacggcggga tacccctgcg catcttccgg cacctggtgc
    31141 atagccggaa tagggaccag cggccctaaa aaacgcggtt cacgtttaaa aatataaagg
    31201 tcagccagcg caccgaaacg ggcggcaaat tcgcgaatac gtactgacag catatctttc
    31261 ggtgacggta cggcataaca ctgagcctga atccccatat gcagcgcaat aaataatgct
    31321 cgctcacagt ggaaacgttg ggtgataata atgaaatcat tagtatcgaa aactttgcgt
    31381 gtacgcacga tggaatccag cgtacgaaag cctgcgtaat cgagaacaat atctgatggg
    31441 tcgacaccag cagcgattaa atctttgcgc atggtcatcg gctcgttata actttgcaat
    31501 gcgttatcgc cgctcagtaa tagataattt accttaccgc tgttataggc attaatcgct
    31561 ccttgaatgc ggtagcgata atactgatta ataacgccgg tacgataata ttttgctgtg
    31621 ccgagcacca cgccgacctg gcggtagggg agatcctgca attcgtcgta gatataaggc
    31681 gcggttttcc agctcatcca gcgatcgagg ccgagcacag tcaacagcag caagccgatc
    31741 aggactaaca ggctgaggaa cacgcgcttt aacatgcagg agactcgagc taaggaaaat
    31801 ttccatcagg ctactttacc cgctggtttt gcgcaagaaa cggcaaactg tttgccggga
    31861 atttcgacac caggagaggg ctggcaagcc ggacaaggcg ttcacgccgc atccggcacc
    31921 aggcttaagc gagaagcacg cggtcgatac tgtgacagcc gagagctttc agcgttgcgg
    31981 aggtggcatc ccactgaatc aacggcgcat cggctttttc tgccagaacc gcgcgctgga
    32041 tatccgggta atcataaccc ttgagattca acagaattaa cgccccctgc aacggcggca
    32101 gagtcgggtt gaatgcggcg ttttcagcgt agctgccgct gaaaatacga ccgtctttac
    32161 attccagcgc gacaccgctt ggtgacttac tgtaaggcat gtgcgaacgg tttgccgctg
    32221 caatcgccgc ctgagaaagc gcatcaccag tcagcgcata gccgtgatcc tgctcgtcca
    32281 tcagcagcgt tttaatctcc agatctttcg gcccaaaggc atccggcaga tagtcacgca
    32341 gcgcgtgtgc ctcgcggccc ggcaaatgaa tacgcagatc cagaccgctg ttcagttcat
    32401 tcataaactg acggcagtga ccacaaggcg tatagttaac ggtgatggct gcaagcgctt
    32461 tttcaccact caaccaagcg tggctgatcg cgctttgttc ggcatgaacg gtttgctgca
    32521 ttgttgcacc aataaattcc atattggcac caaaatacca ggttccgctc acaccgcgcg
    32581 caattgcgcc aacattaaaa ttcgacaatg gcgtacgcgc acaggccgcc gccagcggaa
    32641 gtagtgcgaa tgccagcgcg tcttcgtcca gtcccgttgc gctcttcagc gatgagactt
    32701 gctccccggt caacaaagcg gggaagtact tgtctgccag aataggttcc agtgcagatt
    32761 gcaagttatc cgcaagttgg gcaaaagcgg tttgaaaacg tggatgcatg gcgttgcctc
    32821 ataatcatgt aatgggataa cagtttacga acccgttatg gctttatatg tgatctgaat
    32881 ctcattaatt atgcaacacg tgaaacttac atcaaaaatg cgcgacgtat cgcaaatttt
    32941 agcccattac tgccagaata atcgggaaaa ggaacggtgc gatcagcgaa gtaattatcc
    33001 cgcataacac cagcgctagc gaactaaatg caccttcctg ataatccagc tcggcgcagc
    33061 gcgccgtacc gagggcgtgc gaggcagtcc ccattgccag accgcgcgca gctttggtac
    33121 gaatacgcat cgcattaagc aatgtatggc caaataccgc gccgaggatg ccgacgaaaa
    33181 tcacgcaaac tgcgctgatt gccggaatac cgccaatact gccgccaacc gccattgcaa
    33241 taggcgtggt gactgatttc ggcaggattg acgcggcgat ttccggtgaa gcgcccatca
    33301 acaatgccac ggaagtcccc gtcaccatcg caaccacgct gccgataaaa cagatggtga
    33361 tgatcgattt ccagcgcgcg cggatctggt gtagctgctc atataaagga taggccagcg
    33421 cgacgaccgc cggttgcagc agatcgttta gcacttcgct acctttaaag taactgtcgt
    33481 aagagatgcc ggtcagcatt aaaaaaggaa tgatcaccac cattgctacc agcaacgggt
    33541 taagcagggg aaacttatac cgtgccgcca gtttgcgggc ggcaaaaaag acaatcaacg
    33601 tcagcggtaa tgaccaccag atattcgcca tcattcttct gatcctttct gacctacgac
    33661 tttacgttca ccgtgtacca gttgtgaact ccagctcacc accagaaaaa ccaccagcgt
    33721 actgacagca caggaaacca ctaccgggcc aaactgcgcg cggagcaaat caaaatattg
    33781 catgacgcct acgccaatcg gcacaaacaa tagcgccata tagcgaatca gtacgtagca
    33841 ccccggattg acccatttcg ccggaagaat ttgcaaggcc agcaggacaa acaggatcag
    33901 catcccgatg atgctgcccg gaatggttac cggtagcagg gaagcaatga aaatgcctgc
    33961 atacaggcag gcataaatca ggacgaaagc gcgtaaatat tgccagataa tgttcagtgt
    34021 cttactcata atcaacaccc cagatgagat ggattcatca tacaattaat tcgtaaaacg
    34081 tgctaccgat cacattaaga aatcagcgct tattttttcg ctacccggta ttacgcaatg
    34141 agcagcgaac tcaggcatac atcaagcata gagagggatt gcagtttgca gcaatccctc
    34201 tttgcagacc tttaatccgc tgaccatagg cgctaacata gcgcctcatt tttttgcggg
    34261 tgatgatatg cgtgtgttac tggcaccgat ggagggagtg cttgactctc tggtgcgtga
    34321 attgctgacc gaagttaacg actacgatct gtgcatcacc gagtttgtcc gcgtggtgga
    34381 tcaactgctg ccggtaaaag tctttcatcg catttgccct gagctacaaa acgccagccg
    34441 gacaccatct ggtacgctgg tgcgcgtgca gttgttaggt cagttcccac aatggctggc
    34501 agagaacgcc gcccgtgcgg tggagttagg tttctggggc gtggatctca attgcggctg
    34561 cccgtcgaaa acggttaacg gtagcggcgg cggggcgacg ttactcaaag atcctgaact
    34621 catctaccag ggggcaaaag cgatgcgtga agctgtaccg gcgcatttgc ctgtcagcgt
    34681 gaaagtgcgt ctgggctggg acagcggtga gaagaaattt gaaatcgccg atgcggttca
    34741 acaggctggc gctacggagc tggtggtgca tgggcggacg aaagagcagg gttaccgcgc
    34801 ggagcatatt gactggcagg cgattggcga gattcgccag cggctgaata ttccggtgat
    34861 tgccaacggt gaaatctggg actggcagag cgcgcaacaa tgcatggcga tcagcggctg
    34921 cgacgcagtg atgattggtc gcggggcgct caatattccc aacctgagcc gggtggtaaa
    34981 atataacgaa ccgcgaatgc cgtggccgga ggtggttgct ttgctgcaaa aatatacccg
    35041 tctggaaaag cagggcgata ccgggttata tcacgttgcg cggattaaac agtggttgag
    35101 ttatttgcgt aaagaatacg atgaagcaac ggaattattt cagcatgttc gggtgttgaa
    35161 taactcccct gatattgcaa gggctattca ggcaattgat atcgagaaac tctaaaaacc
    35221 atagtgatgt ttttgacaat gtgttagctc tttatcaata ggccgcacgg ttgtttggtg
    35281 gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga tgtgggctga
    35341 gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag ggataagccg
    35401 agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggggcaggg ggcggcggcg
    35461 attggtcgcc ccctgcgcgc tccttgcgcc agtgattata tgaagcaatg cacatgacag
    35521 gagcgcaaca ggatgagaga ctgaaatatt ctcgttattt tcggatctga tgacattaat
    35581 taattttaaa atatcatctt aaatacgcca gtcaccacca gtagcccaat caggaaaata
    35641 atcaatacag cccagagtat aattttcatt ggcgttcctt tatttgtgag tgctgaataa
    35701 gtataggaaa aaacgaagcc ctttgccgtt atccataaac gattaagata aatcggaaga
    35761 tatcatttac ttagtctata tttttctggg tatgtcgttt tattgctact gatgtattgc
    35821 cacgcgaaac taaaaaatat atcgcaacac acggtaatct ccgcgcaccg tttcttacct
    35881 gattattccc ccatgaatcg tgattccttt tatccagcca tcgcctgttt tccgctgtta
    35941 ctgatgctgg ccgggtgtgc gcctatgcat gaaacccgcc aggcgttaag ccagcaaacg
    36001 cccgctgcac aagttgacac cgcattaccc acggcgctga aaaatggttg gccagacagc
    36061 caatggtggc tggagtatca cgataatcag ctcacttcat taattaacaa tgcactacag
    36121 aacgcaccgg atatgcaggt ggctgaacag cgcatttagc tagctgaagc ccaggcgaag
    36181 gcagttgcca ctcaggatgg tccgcagatc gacttttcgg cggatatgga gcggcaaaaa
    36241 atgtcggcag aagggctaat ggggccgttt gctctgaacg atccggccgc aggtacgacc
    36301 ggtccgtggt acaccaacgg aacttttggc ttaacggcgg gctggcatct cgatatctgg
    36361 ggaaagaatc gggcggaggt tactgcccgc ctgggtacgg ttaaagcacg ggcggcggaa
    36421 cgcgagcaaa cccgccaatt gctggctggc agcgtagccc gcctgtactg ggagtggcaa
    36481 acccaggcgg cgttaaacac ggtcttgcag caaatagaaa aagagcagaa caccattatc
    36541 gcgaccgatc gccagctata tcagaacggg attacttctt cagttgacgg tgtggaaacc
    36601 gatattaatg ccagcaaaac ccggcagcag ctcaacgatg tcgcggggaa aatgaaaatt
    36661 attgaggcac ggttaaatgc actcacaaat caccagacta agtcattgaa gcttaaaccg
    36721 gtcgcgttgc cgaaagtggc aagccagctt cctgatgaac tggggtactc cttactggcc
    36781 cggcgggcag atttgcaggc ggcgcactgg tacgttgagt catcgttaag cactattgat
    36841 gcggcaaaag cggcatttta tcctgacatc aacctgatgg ccttcctgca acaggatgcg
    36901 ttgcacttaa gcgatctgtt ccgtcattcc gcgcagcaaa tgggcgttac ggcaggcctg
    36961 acgctaccta ttttcgatag tggtcgtctt aacgccaatc tcgatatcgc aaaagccgaa
    37021 agcaacttgt ctatcgccag ctacaacaaa gcggtggttg aagcagtgaa tgacgtggcg
    37081 cgggcagcca gtcaggttca gacactggcg gagaaaaacc agcatcaggc gcaaattgag
    37141 cgcgatgcct tgcgtgtggt aggtcttgcg caggcccgct ttaacgcggg catcattgct
    37201 ggttcccgcg tcagcgaagc cagaatcccg gcgctgcgtg agcgggccaa tggcctgtta
    37261 ttgcaagggc agtggctgga tgcctccatt cgactcaccg gtgcgctggg cgggggttac
    37321 aaacgctgat gatatgcgcc ttctatactt aacgtttatt cagcgttaag tggagaactc
    37381 gatggcatag gtcgcgataa ttaccgcttc cgattcgggg atcggcaaag agtgcgcgtt
    37441 attactggcg cagcaggggt ttgatattgg cattacctgg cactcagatg aagaaggggc
    37501 aaaagatacc gcgcgtgagg tggttaccca cggcgtacgt gcggagatcg tgcaactgga
    37561 tctcggcaat ctgccagaag gtgcgcaggc gctggagaca ctcatccagc gatttgggcg
    37621 tattgatgtg ctggtgaata atgcgggtgc aatgaccaaa gcgccgtttc ttgatatggc
    37681 ttttgatgag tggcgcaaga tttttaccgt tgatgttgat ggtgcatttt tatgctcgca
    37741 aattgcggct cgtcagatgg tgaaataagg gcagggcggt cgcatcatca acattacgtc
    37801 ggtacatgaa catacgccgc tgccggatgc cagcgcctac acagccgcta aacatgcgct
    37861 cggcgggtta accaaaacga tggcgctgga gttggtcagg cataagattt tggtgaacgc
    37921 agtcgcgcct ggggcgatag ccacgccaat gaacagcatg gatgatagcg acgtgaagcc
    37981 cgacgcggag ccttcgattc ctctacggcg ttttggcgca acgcatgaga ttgccagcct
    38041 ggtggtgtgg ctttgttcgg agggcgcaaa ttacacgaca gggcagtcgt tgatagtgga
    38101 tggcggcttt atgttggcga atccgcagtt caacccagaa tagcgcagtg aggtgcgtgg
    38161 atatttcaat gggttaggcc agataagaca ccagcgtcgc atcaggcatg gaacacccgg
    38221 tgccggatgt ggagtgaatg cttatctgtg gcaactgctt tacgcctgat tatccggctt
    38281 tttcttcccg cgccgtttca gccaccagcg cacgcccacc accagaacca cgaccagaat
    38341 caaccagacc cagtgcttca aatgctggtc gagattgtgc aaccacggcg caatcacctg
    38401 accaccagcg taaccaatag tggtaaaaat caacgcccag gcaaatgcgc cgagaatatt
    38461 cagcggcaga aagattttcg gcggcagctg gctggcacca atcagcgtcg ggccaatcac
    38521 ccgaaagcca tacataaagc gcgtaccaat gacaaacaga tacggatggc gttggataag
    38581 tttctgtgcc cgctcaattt tatcctgatg tttcgagaaa cggcgtaaca gcttgccgcc
    38641 aaaccgccgc ccgcacagat agagcacctg gtcgccaatc atgccaccaa gcgccacaga
    38701 aagtaccacc agtgggaact ttaatagccc ctgatgtgcc gcaacgcctc ccagcaaagt
    38761 cacggtttca ccttccgcca ggctaccgat aaccagcgcg gcataaccat attgtgagat
    38821 gagtgtattg agatccatag gccagtttgt ctccctttgg ttgcctgtaa gcatacaccc
    38881 tgtgaatcat tcacgctgaa aaaggacata catcagacgg tgcaaattac atggatgaac
    38941 aaaaaataat cacaagctgg cttatacttg aactgtcagg ttcatgaact cacaacaagg
    39001 gggcggtatg agccatgtct ggggactgtt ttcccatccc gatcgtgaaa tgcaggtgat
    39061 taatcgcgaa aacgaaacga tttctcacca ttacacccac cacgttttgc tgatggcggc
    39121 gattccggtg atttgcgcct tcattggcac tacacagatt ggctggaatt ttggcgatgg
    39181 cactatcctg aagttatcct ggtttaccgg actggcgctg gctgtcttgt tttacggcgt
    39241 tatgctggct ggggttgcgg tgatggggcg ggtcatctgg tggatggcgc gcaattaccc
    39301 gcagcgtccg tctcttgcgc actgcatggt cttcgcgggc tacgttgcaa ctccgctgtt
    39361 tttaagcggt ctggtggcgc tttatccact ggtctggttg tgtgcgctgg tcggcacggt
    39421 ggcgctgttt tacaccggct acctgcttta ccttggtatt ccgagcttcc tgaatatcaa
    39481 caaagaggaa ggccttagct tctccagttc gacgctggcg attggcgtgc tggtactgga
    39541 agtgctgctc accctaaccg ttatactatg gggctacggt tatcgtttgt tctagaaagt
    39601 tctttgacgt tgcattgctg gcgtaaatgt cagcaatgca gcatttactc acgattctcc
    39661 tttgacgatc gcactttttg ctcgttatga tggcgtcgct agcctcagta aatccttact
    39721 ccggcggtgc gcaacccgtg cgcgtgaacc actatctgaa tgctcatcat gccgaaattt
    39781 cgagtttctt tatttagctt ggccctgatg ctggctgtgc cttttgcacc gcaggccgtt
    39841 gcgaaaacgg cagccgctac caccgcttca caaccggaaa ttgcctccgg tagcgcgatg
    39901 attgttgatc tgaataccaa caaagtgatc tattcgaacc acccggatct ggtgcgtccg
    39961 attgcgtcta tcagcaaatt aatgaccgcg atggttgtgc tggatgcacg actgccgctg
    40021 gatgaaaaac taaaagtgga tatcagccag acgccggaga tgaaaggggt ctattcgcgc
    40081 gtacgactga atagcgaaat cagccgtaaa gatatgctgt tgctggcgct gatgtcttca
    40141 gaaaaccgcg cggcggcaag ccttgcgcac cattatcccg gtggttacaa agcctttatt
    40201 aaggcaatga atgcgaaagc gaaatcgctc ggaatgaaca acacgcgctt tgttgaacct
    40261 accggattgt cggtgcataa cgtttcaact gcccgtgact taaccaaact gctcattgcc
    40321 agcaaacaat atccgttgat cgggcagtta agtactaccc gggaagatat ggcaaccttc
    40381 tctaacccga cgtatacgct gccgttccgc aatactaatc atctggtgta tcgcgataac
    40441 tggaatattc agttaaccaa aaccggcttt accaatgcgg cgggccattg tctggtgatg
    40501 cgtacggtta tcaataataa accggtggcg ctggtagtga tggacgcgtt tggcaaatat
    40561 acccattttg ccgatgccag ccgcctgcgt acctggattg aaaccggtaa agtgatgcct
    40621 gtgccggcag cagcgttgag ctataaaaaa caaaaagccg cccaaatggc ggcggcgggg
    40681 cagacggcac agaacgatta agtgaattcg gatggcgata ctctgccatc cgtaattttt
    40741 actccacttc ctgccagttt ttccgtttac tggttttacc gatccccgga ttcatgctat
    40801 tggtcggatc attctcgcga tagaacttct gcaacgtctc cggtgcttta tacaaatgac
    40861 cgacgttatg ctcggcaggg tactgcgcgc cgcgctgctg tagcagttcc agcatctgtt
    40921 cttttaacgc atgcacatcc acgccttttt tcactatgta atcctgatgg aagacatagc
    40981 acataaaatg gccgtaatag agcttgtgca ccagctggct gtcgatctcc ggcggtaaat
    41041 gctcatacca ctcggtgtcg ttacgccgca gagcgatatc cagtgccaga atgtcttcga
    41101 cttcatcgga atgcaccgcc tgataacgaa ttgctgcgcc cgcagcggcg aaacggtgta
    41161 aaaacgcttt gctaccttcc tccggcgtac agacaaagaa atcgccctcg gcctgtttga
    41221 aataatccac cagccacgat ttggcttcac cgacgccatc gcctgccatt tttaacagca
    41281 gatgatgctc gtatttatcg cgccagtttt tcatgcgcgg cggtaaatgg ctggggaaca
    41341 ggtgaccgaa tttttgcatt gcacggtcag taaaatgcgg acggaagaat ttcactttct
    41401 ccagcatcgc atcggtgcgt cccttgagat taaagaagaa cggcatcttg tcggtgccta
    41461 acttatcaat catcaggaag gtgtctttgc cgtatttttc cgcaatatcg tagatatccc
    41521 ggtgcatata ttccccggca accggcagat tttcgaagtt agccagaata tgacggcgga
    41581 tttcggtcag cacttccggc tggttggtgc cgatataaaa cacctgctga tttttttccg
    41641 cttcgaaggt atcaagacgt accgcaaaga cggccagctt cccggcgcaa ccagaagatt
    41701 caaataaccg atccggatcg gcgttataac gtgcgggcgt gtcggcttca atatcacgaa
    41761 cgcggtggac atagtcataa tcgtgggcgt gacgaccatc gtgacgcaca tcgtcatctt
    41821 tgatgcgatc gtcatccagc ttgctaagga tctgctccgg cgtttcgccc agatcaatcc
    41881 ccagatggtt caccagcgtc agtttgccat cttcatctat acgcgcgaat aacgacattt
    41941 cggtatacgc cgggccacgt tgcaccagcg agccgccgga gttgttacaa ataccgccga
    42001 tgaccgatgc gcctatacac gatgatccaa tcaccgaatg cggttcgcgt cccagcggtt
    42061 tgagggcttt ttccagcgaa tagagcgtgg tgcccggata ggccagcacc tgttcgccct
    42121 tgccgagaac gtgcagcttg tcgagacgca gggtgctgat gatcacgata tcgcgatcat
    42181 agtcgttacc gtttggcgtc gatccttcgg tcaggcctgt attggcagcc tgcatcagaa
    42241 taattttgtc ggcggtgacg caggctttca gcacccgcca caattccagt agtgagccag
    42301 ggaaaacgac agccagcgcg tcgccctgac cagaacggaa gcccttgcga tagcgggccg
    42361 tttttgcggg atcggtgagc aggtgtgaat gaccgaccag acgggcaagt tcattcaaaa
    42421 aggctttatt atcagttgtt gtcatggaag acattttcca ctccttgtgg tggcgaaaaa
    42481 atatcacaaa ctagaatagc gccttgtatg acaattcagc gagtgaatat tttgaaaatt
    42541 cagacaataa actgagcatt ctttttctct ctccatcatg cttatggcaa actacggttt
    42601 ttgcaccatt ttcgctgcca cgtcgggcaa caaaggaaga aaaatccata tgaaatggct
    42661 atgttcagta ggaatcgcgg tgagtctggt cctgcagcca gcactggcgg atgatttatt
    42721 cggtaaccat ccattaacgc ctgaagcgcg ggatgcgttc gtcaccgatc tgctcaagaa
    42781 aatgacggtt gatgagaaaa ttggtcagct gcgcttaatc agcgtaggcc cggataatcc
    42841 gaaagaggcg atccgcgaga tgatcaaaga cggtcaggtt ggggcgattt tcaacaccgt
    42901 aacccgtcag gatatccgcg ccatgcagga tcaggtgatg gaattaagcc gcctgaaaat
    42961 tcctcttttc tttgcttacg acgtgctgca cggtcagcgc acggtgttcc cgattagcct
    43021 cggtctggcc tcgtctttta acctcgatgc ggtgaaaacg gtcgggcgtg tctctgctta
    43081 tgaagcggca gatgatggcc tgaatatgac ctgggcaccg atggtcgatg tctcgcgcga
    43141 tccgcgctgg ggacgtgctt ccgaaggttt tggcgaagat acgtatctca cctcaataat
    43201 gggtaaaacc atggtggaag cgatgcaggg taaaagcccg gcagatcgtt actcggtgat
    43261 gaccagcgtc aaacactttg ccgcatacgg cgcggtagaa ggcggtaaag agtacaacac
    43321 cgtcgatatg agtccgcagc gcctgtttaa tgattatatg ccgccgtaca aagcggggct
    43381 ggacgcaggc agcggcgcgg tgatggtggc gctgaactcg ctgaacggca cgccagccac
    43441 ctccgattcc tggctgctga aagatgttct gcgcgaccag tggggcttta aaggcatcac
    43501 cgtttccgat cacggtgcaa tcaaagagct gattaaacat ggcacggcgg cagacccgga
    43561 agatgcggtg cgcgtggcgc tgaaatccgg aatcaacatg agtatgagcg acgagtacta
    43621 ctcgaagtat ctgcctgggt tgattaaatc cggcaaagtg accatggcag agctggacga
    43681 tgctgcccgc catgtactga acgttaaata tgatatgggg ttgtttaacg acccatacag
    43741 ccatttgggg ccgaaagagt ctgacccggt ggataccaat gccgaaagcc gcctgcaccg
    43801 taaagaagcg cgtgaagtgg cgcgcgaaag cctggtgttg ctgaaaaacc gtctcgaaac
    43861 gttaccgctg aaaaaatcgg ccaccattgc ggtggttggg ccactggcgg acagtaaacg
    43921 tgacgtgatg ggcagctggt ccgcagccgg tgttgccgat caatccgtga ccgtactgac
    43981 cgggattaaa aattccgtcg gtgaaaacgg taaagtgctg tatgccaaag gggcgaacgt
    44041 taccagtgac aaaggcatta tcgatttcct gaatcagtat gaagaagcgg tcaaagtcga
    44101 tccgcgttcg ccgcaagaga tgattgatga agcggtgcag acggcgaaac aatctgatgt
    44161 ggtggtggct gtagtcggtg aagcacaggg gatggcgcac gaagcctcca gccggaccga
    44221 tatcactatt ccgcaaggcc aacgtgactt gattgcggcg ctgaaagcca ccggtaaacc
    44281 gctggtgctg gtgctgatga acgggcgtcc gctggcgctg gtgaaagaag atcagcaggc
    44341 tgatgcgatt ctggaaacct ggtttgcggg gactgaaggc ggtaatgcaa ttgccgatgt
    44401 attgtttggc gattacaacc cgtccggcaa gctgccaatt tccttcccgc gttctgtcgg
    44461 gcagatcccg gtgtactaca gccatctgaa caccggtcgt ccgtataatg ccgacaagcc
    44521 gaacaaatat acttcgcgtt attttgatga agctaacgga gcgctttatc cgttcggcta
    44581 tggtctgagc tataccactt tcaccgtctc tgatgtgaaa ctttctgcgc cgaccatgaa
    44641 gcgtgacggc aaagtgacgg ccagcgtgca ggtgacgaac accggtaagc gcgagggggc
    44701 cacggtagtg cagatgtact tgcaggatgt gacggcttcc atgagtcgcc ctgtgaaaca
    44761 gctgaaaggc tttgagaaaa tcaccctgaa accgggcgaa actcagactg tcagcttccc
    44821 gatcgatatt gaggcgctga agttctggaa tcaacagatg aaatatgacg ccgagcctgg
    44881 caagttcaat gtatttatcg gcactgattc cgcacgcgtt aagaaaggcg agtttgagtt
    44941 gctgtaatgc tttagtaagg gcgcgacgtt tgcgcccttt gcctgatgtt catatcttaa
    45001 cttgctgaat tttcgccctt tgtaggccgg ataaggcgct cgcgccgcat ccggcaggaa
    45061 acgccggacc gaatatgcct cttaagtatt tatctgattc tgcaactgaa tccttccgct
    45121 caggctaact ctgtcattat caactatgct tttctcttaa ttcgctgaaa acaacagtaa
    45181 aagaatgagg aaagcatcat gccactctta aagctctggg caggttcact ggttatgttg
    45241 gcagccgtga gcctgccgct acaggcggct tcccccgtta aagtcggttc aaaaatcgat
    45301 accgaaggcg cgctgctcgg caacatcatc ttgcaggtac tcgaaagcca cggcgtaccg
    45361 acggtgaata aagtgcaact cggaacgact cctgtggggc gcggggcgat tacttccgat
    45421 gaactggata tctatccgga atataccggc aatggcgctt tcttctttaa agatgaaaac
    45481 gatgcagcgt ggaaaaatgc cgggcagggc tacgagaaag tcaaaaagct cgatgcggag
    45541 cacaacaagt taatctggct gacgcccgcg cctgcaaata acacctggac tatcgccgtg
    45601 cgtcaggatg tggcagagaa aaacaaactc acttcgcttg ccgacctgag tcgttatctg
    45661 caagagggcg gcaccttcaa actggcagcc tcggcagagt ttatcgaacg cgccgatgcg
    45721 ttacccacgt ttgaaaaagc ctatggcttt aagctcggtc aggatcagtt gctgtcactg
    45781 gctggtggcg acacggcggt gacgattaaa gccgctgccc agcaaacctc tggcgttaat
    45841 gccgcaatgg cttacggcac tgacggtccg gtcgcggcgc tggggctgca aaccttaagc
    45901 gatccgcaag gcgtgcaacc tatctacgcg cctgcaccag tggtgcgtga atcggtgttg
    45961 aaggagtatc cgcaaatggc acagtggcta cagccagtct tcgccagcct cgatgcaaaa
    46021 acattgcagc aactgaatgc cagcattgca gtggaaggac tggatgccaa aaaagtggct
    46081 gccgactacc tgaaacaaaa agggtgggcg aagtaatttc ccgtgactta tctacgtatt
    46141 aatcctgttc tggcgctgct gttgttgctg acggcaatcg cagcggcgct gccgtttatc
    46201 agttacgcgc ctaatcgttt agtctcgggt gaagggcttc atctctggca actgtggccg
    46261 caaacgatct ggatgctggt gggcgttggt tgcgcctggc tgacagcctg ttttgtcccc
    46321 gctaaaaaag gcagcatttt tgcactcatt ctggcgcaat tcgtcttcgt attgttggtg
    46381 tggggagctg gaaaggcggc gacccaactg gcgcaaaatg gcagtgcgct ggcgcgtacc
    46441 agcctcggca gaggtttctg gctggctgcg gcgctggcat tgctggcctg tagcgatgcc
    46501 atccgccgaa tctccacgca tccgctgtgg cgctggttgt tgcatatgca gattgccatt
    46561 attccgctgt ggttgctgta ctccggcacg cttaacgatc tctcactaat gaaagaatac
    46621 gccaaccgtc aggatgtgtt tgacgacgcg ctggcacaac atctgacgtt gctgtttggt
    46681 gcggtgctgc ctgcgttagt gattggtgtg ccgttgggca tctggtgcta cttttccact
    46741 gcgcggcagg gggcaatttt ttctctgctc aatgtcattc agaccgtgcc ttcggtggcg
    46801 ctctttggcc tgttgattgc gccgcttgcc gcgctggtta cggcctttcc gtggctgggg
    46861 aagctcggca tagcaggaac cggaatgaca cccgcactga ttgcgctggt gctctatgcc
    46921 ttgctgccgc tggtgcgagg cgtggtagtc ggtttgaacc agatcccgcg cgatgtgctg
    46981 gagagcgcca gagcgatggg gatgagcggg gcgcagcgtt tcctgcatgt tcagttaccg
    47041 ctggcgttac cggtatttct gcgcagcctg cgggtggtga tggtgcaaac tgtaggtatg
    47101 gcggtgattg cggcgttaat cggcgcaggc ggttttggtg cgctggtttt ccaggggctg
    47161 ctaagcagcg ccattgattt agtgttgctg ggggtgatcc cggtaattgt tctggcggtg
    47221 cttaccgacg cgctgttcga tttgcttatc gcactgctga aggtgaaacg taatgattga
    47281 atttagccat gtcagcaaac tgttcggcgc acaaaaagcc gttaacgatc tcaatctcaa
    47341 ttttcaggaa gggagttttt cggtgctgat tggcacatct ggctccggca aatccaccac
    47401 cctgaaaatg attaaccgcc tggtggagca tgacagcgga gtgatccgct ttgccggaga
    47461 agaaattcgc tcgctgccag tactggagtt gcgccgccgg atgggctatg ccattcaatc
    47521 tattggcctg ttcccccact ggagcgtggc gcaaaacatc gctaccgtgc cgcaattaca
    47581 aaaatggtcg cgggcgcgga ttgacgatcg tatcgacgaa ttaatggcgc tactggggct
    47641 ggagtcaaat ttgcgtgagc gttatccgca tcagctttcc ggtggtcagc agcaacgtgt
    47701 gggagtggcg cgtgcactgg ctgccgatcc gcaagtctta ctgatggatg aaccttttgg
    47761 cgcactggac ccggtaacgc gcggcgcgtt gcaacaagag atgacgcgca ttcaccgttt
    47821 gctggggcgc actattgtgc tggtcactca tgatattgat gaggcgctac ggctggcaga
    47881 acatctggta ttgatggatc acggtgaagt ggtgcagcag gggaatccgc tgacgatgct
    47941 gactcgtccg gcgaatgatt ttgtccgcca gttttttgga cgtagtgaac tgggggtgca
    48001 cctgctttcg ttacgtagcg tggcggatta cgtgcgtcgc gaagaacggg cagaaggtga
    48061 ggcactggca gaagagatga cgctacgcga tgcgctctcc ctgtttgtcg cgcggggatg
    48121 cgaggtgctg ccggtggtga acacgcaggg ccagccttgc ggcacgctgc attttcagga
    48181 tctgctggtg gaggcgtaag cgtatgaaga tgttgcgcga tccgctgttc tggctcattg
    48241 ctctttttgt ggcgctgatt ttctggctgc cttacagcca gccgctgttt gctgccttgt
    48301 tcccacaact gccacgaccc gtttatcagc aagaaagttt tgcagctctg gcactggctc
    48361 atttctggct ggtgggaatt tcgagtttgt ttgcggtgat cattggcaca ggtgccggaa
    48421 ttgctgttac tcgcccgtgg ggcgcggaat ttcgcccact ggtggaaact attgccgccg
    48481 ttggacagac ttttccgcct gtcgcagtgc tggcgattgc cgttccggtg atcggctttg
    48541 gtctgcaacc agcgattatc gccttgatcc tttacggtgt gctgcccatc ctgcaggtga
    48601 cacttgccgg gctgggagcg attgatccca gcgtgacaga agttgcgaaa ggtatgggaa
    48661 tgagtcgagg tcagcgactg cgtaaggtcg agctaccgct ggcggctccg gtgattctgg
    48721 cgggcgtgcg aacttcggtg attatcaaca ttggtacggc gacgatcgcc tcaacggtag
    48781 gggccagtac gctgggtacg cccatcatca tcgggcttag cggatttaat accgcgtatg
    48841 tgatccaggg ggcgttactg gtggcactgg cggcgatcat cgcagaccgc ctgtttgaaa
    48901 ggctggtgca ggcgcttagc cagcacgcaa aataaaggta taacctgcga gcatgacgcc
    48961 accaattccg cctaacgcca taaacaggaa cagggcgatg accccaattt tagctatgcg
    49021 cataatgcac tccttatgtt aacgaaagga ttgtacagta aagcgcattt gttaacgaat
    49081 cattaaatac cgagtgggaa aatatcatgg ccttgttctt gccaactggt gagttgctgc
    49141 tgttgggcgg aggttcgatt ttcaccgcac cacaccagca atgtacggcc ttcgaatagt
    49201 tcagggcgta gttgattgag cgagtgggcg agcgaggaca tcaatgcgcc atccttgttg
    49261 actggcaatc cagccctcca gccacagacg ggtggtatcc tgaatattcc agccaaccac
    49321 cagcgcatct ttaccctgtt ttttacgtgc cgaagccaga caaatggcga tgtagttgat
    49381 cagtacgccg tcgaggatcg ccagcagcgc ctggagagtc ggttgttggc actgaagccg
    49441 tcggcgcaga ggaataaaca gatgtgtggt gagtgtctgg gcggggtaat cctgaccgcg
    49501 ctctttgatc cacgttcgca ggctatgtag attgc
//
LOCUS       DAJVQR010000021        48322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVQR010000021 DAJVQR010000000
VERSION     DAJVQR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            187..480
                     /locus_tag="OQM52_001271"
     CDS             187..480
                     /locus_tag="OQM52_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1563192.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OQM52_001272"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OQM52_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1563193.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OQM52_001273"
     CDS             complement(1922..2374)
                     /locus_tag="OQM52_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1563194.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OQM52_001274"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OQM52_001274"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1563195.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OQM52_001275"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OQM52_001275"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1563196.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OQM52_001276"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OQM52_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1563197.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OQM52_001277"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OQM52_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1563198.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OQM52_001278"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OQM52_001278"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1563199.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OQM52_001279"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OQM52_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1563200.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OQM52_001280"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OQM52_001280"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1563201.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OQM52_001281"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OQM52_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1563202.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OQM52_001282"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OQM52_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1563203.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OQM52_001283"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OQM52_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1563204.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OQM52_001284"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OQM52_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1563205.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OQM52_001285"
     CDS             complement(15085..15582)
                     /locus_tag="OQM52_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1563206.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OQM52_001286"
     CDS             complement(15579..15941)
                     /locus_tag="OQM52_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1563207.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OQM52_001287"
     CDS             complement(15931..16278)
                     /locus_tag="OQM52_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1563208.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OQM52_001288"
     CDS             16387..16800
                     /locus_tag="OQM52_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563209.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OQM52_001289"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OQM52_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OQM52_001290"
     CDS             complement(18324..20039)
                     /locus_tag="OQM52_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCS1563210.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGVEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OQM52_001291"
     CDS             20206..21099
                     /locus_tag="OQM52_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1563211.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OQM52_001292"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OQM52_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="OQM52_001293"
     CDS             complement(21910..23526)
                     /locus_tag="OQM52_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1563212.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="OQM52_001294"
     CDS             23821..24495
                     /locus_tag="OQM52_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1563213.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="OQM52_001295"
     CDS             complement(24534..26195)
                     /locus_tag="OQM52_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1563214.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OQM52_001296"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OQM52_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1563215.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OQM52_001297"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OQM52_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1563216.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="OQM52_001298"
     CDS             27649..27981
                     /locus_tag="OQM52_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1563217.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="OQM52_001299"
     CDS             complement(27983..29215)
                     /locus_tag="OQM52_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1563218.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="OQM52_001300"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="OQM52_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS1563219.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="OQM52_001301"
     CDS             complement(30377..31714)
                     /locus_tag="OQM52_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1563220.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OQM52_001302"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OQM52_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OQM52_001303"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OQM52_001303"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS1563221.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OQM52_001304"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OQM52_001304"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS1563222.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OQM52_001305"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OQM52_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS1563223.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /locus_tag="OQM52_001306"
     CDS             35377..36033
                     /locus_tag="OQM52_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563224.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OQM52_001307"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OQM52_001307"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS1563225.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="OQM52_001308"
     CDS             complement(37005..37403)
                     /locus_tag="OQM52_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS1563226.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OQM52_001309"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OQM52_001309"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1563227.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OQM52_001310"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OQM52_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1563228.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OQM52_001311"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OQM52_001311"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1563229.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OQM52_001312"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OQM52_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1563230.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="OQM52_001313"
     CDS             43964..44044
                     /locus_tag="OQM52_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563231.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="OQM52_001314"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="OQM52_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1563232.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="OQM52_001315"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="OQM52_001315"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1563233.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="OQM52_001316"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="OQM52_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1563234.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="OQM52_001317"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="OQM52_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1563235.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="OQM52_001318"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="OQM52_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1563236.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13067 c        12219 g        11056 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 caagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaacg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa aa
//
LOCUS       DAJVQR010000022        47903 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVQR010000022 DAJVQR010000000
VERSION     DAJVQR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(313..930)
                     /gene="tdk"
                     /locus_tag="OQM52_001319"
     CDS             complement(313..930)
                     /gene="tdk"
                     /locus_tag="OQM52_001319"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1563237.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1535..1948
                     /gene="hns"
                     /locus_tag="OQM52_001320"
     CDS             1535..1948
                     /gene="hns"
                     /locus_tag="OQM52_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1563238.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2092..3000)
                     /gene="galU"
                     /locus_tag="OQM52_001321"
     CDS             complement(2092..3000)
                     /gene="galU"
                     /locus_tag="OQM52_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1563239.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3202..4215)
                     /gene="rssB"
                     /locus_tag="OQM52_001322"
     CDS             complement(3202..4215)
                     /gene="rssB"
                     /locus_tag="OQM52_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1563240.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4307..5212)
                     /gene="rssA"
                     /locus_tag="OQM52_001323"
     CDS             complement(4307..5212)
                     /gene="rssA"
                     /locus_tag="OQM52_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1563241.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5325..5783
                     /locus_tag="OQM52_001324"
     CDS             5325..5783
                     /locus_tag="OQM52_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1563242.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5833..6675
                     /gene="purU"
                     /locus_tag="OQM52_001325"
     CDS             5833..6675
                     /gene="purU"
                     /locus_tag="OQM52_001325"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1563243.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6835..6919
                     /locus_tag="OQM52_001326"
     tRNA            6835..6919
                     /locus_tag="OQM52_001326"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6869..6871,aa:Tyr,seq:gta)
     gene            6966..7097
                     /locus_tag="OQM52_001327"
     ncRNA           6966..7097
                     /ncRNA_class="other"
                     /locus_tag="OQM52_001327"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7281..7958)
                     /gene="narI"
                     /locus_tag="OQM52_001328"
     CDS             complement(7281..7958)
                     /gene="narI"
                     /locus_tag="OQM52_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1563244.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7958..8668)
                     /gene="narJ"
                     /locus_tag="OQM52_001329"
     CDS             complement(7958..8668)
                     /gene="narJ"
                     /locus_tag="OQM52_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1563245.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8665..10203)
                     /gene="narH"
                     /locus_tag="OQM52_001330"
     CDS             complement(8665..10203)
                     /gene="narH"
                     /locus_tag="OQM52_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1563246.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10200..13943)
                     /locus_tag="OQM52_001331"
     CDS             complement(10200..13943)
                     /locus_tag="OQM52_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1563247.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14336..15727)
                     /gene="narK"
                     /locus_tag="OQM52_001332"
     CDS             complement(14336..15727)
                     /gene="narK"
                     /locus_tag="OQM52_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1563248.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16066..17862
                     /gene="narX"
                     /locus_tag="OQM52_001333"
     CDS             16066..17862
                     /gene="narX"
                     /locus_tag="OQM52_001333"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1563249.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17855..18505
                     /gene="narL"
                     /locus_tag="OQM52_001334"
     CDS             17855..18505
                     /gene="narL"
                     /locus_tag="OQM52_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1563250.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18506..19900)
                     /gene="ychO"
                     /locus_tag="OQM52_001335"
     CDS             complement(18506..19900)
                     /gene="ychO"
                     /locus_tag="OQM52_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1563251.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20085..20438
                     /gene="ychN"
                     /locus_tag="OQM52_001336"
     CDS             20085..20438
                     /gene="ychN"
                     /locus_tag="OQM52_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1563252.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20482..21177)
                     /gene="chaC"
                     /locus_tag="OQM52_001337"
     CDS             complement(20482..21177)
                     /gene="chaC"
                     /locus_tag="OQM52_001337"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1563253.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21335..21565)
                     /gene="chaB"
                     /locus_tag="OQM52_001338"
     CDS             complement(21335..21565)
                     /gene="chaB"
                     /locus_tag="OQM52_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1563254.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21835..22935
                     /gene="chaA"
                     /locus_tag="OQM52_001339"
     CDS             21835..22935
                     /gene="chaA"
                     /locus_tag="OQM52_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1563255.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23340..23447
                     /locus_tag="OQM52_001340"
     CDS             23340..23447
                     /locus_tag="OQM52_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1563256.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23596..24450)
                     /gene="kdsA"
                     /locus_tag="OQM52_001341"
     CDS             complement(23596..24450)
                     /gene="kdsA"
                     /locus_tag="OQM52_001341"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1563257.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24486..25295)
                     /locus_tag="OQM52_001342"
     CDS             complement(24486..25295)
                     /locus_tag="OQM52_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCS1563258.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPPAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25299..25691)
                     /locus_tag="OQM52_001343"
     CDS             complement(25299..25691)
                     /locus_tag="OQM52_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1563259.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25688..26521)
                     /gene="prmC"
                     /locus_tag="OQM52_001344"
     CDS             complement(25688..26521)
                     /gene="prmC"
                     /locus_tag="OQM52_001344"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1563260.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26521..27603)
                     /gene="prfA"
                     /locus_tag="OQM52_001345"
     CDS             complement(26521..27603)
                     /gene="prfA"
                     /locus_tag="OQM52_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1563261.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27645..28901)
                     /gene="hemA"
                     /locus_tag="OQM52_001346"
     CDS             complement(27645..28901)
                     /gene="hemA"
                     /locus_tag="OQM52_001346"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1563262.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29115..29738
                     /gene="lolB"
                     /locus_tag="OQM52_001347"
     CDS             29115..29738
                     /gene="lolB"
                     /locus_tag="OQM52_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1563263.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29738..30589
                     /gene="ispE"
                     /locus_tag="OQM52_001348"
     CDS             29738..30589
                     /gene="ispE"
                     /locus_tag="OQM52_001348"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1563264.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30740..31687
                     /gene="prs"
                     /locus_tag="OQM52_001349"
     CDS             30740..31687
                     /gene="prs"
                     /locus_tag="OQM52_001349"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1563265.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31812..33491
                     /gene="dauA"
                     /locus_tag="OQM52_001350"
     CDS             31812..33491
                     /gene="dauA"
                     /locus_tag="OQM52_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1563266.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33546..33824)
                     /gene="ychH"
                     /locus_tag="OQM52_001351"
     CDS             complement(33546..33824)
                     /gene="ychH"
                     /locus_tag="OQM52_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1563267.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34102..34686
                     /gene="pth"
                     /locus_tag="OQM52_001352"
     CDS             34102..34686
                     /gene="pth"
                     /locus_tag="OQM52_001352"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1563268.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34803..35894
                     /gene="ychF"
                     /locus_tag="OQM52_001353"
     CDS             34803..35894
                     /gene="ychF"
                     /locus_tag="OQM52_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1563269.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36738..39605
                     /locus_tag="OQM52_001354"
     CDS             36738..39605
                     /locus_tag="OQM52_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1563270.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39705..41624)
                     /gene="dhaR"
                     /locus_tag="OQM52_001355"
     CDS             complement(39705..41624)
                     /gene="dhaR"
                     /locus_tag="OQM52_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1563271.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41852..42922
                     /gene="dhaK"
                     /locus_tag="OQM52_001356"
     CDS             41852..42922
                     /gene="dhaK"
                     /locus_tag="OQM52_001356"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1563272.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42933..43565
                     /gene="dhaL"
                     /locus_tag="OQM52_001357"
     CDS             42933..43565
                     /gene="dhaL"
                     /locus_tag="OQM52_001357"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1563273.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43576..44994
                     /gene="dhaM"
                     /locus_tag="OQM52_001358"
     CDS             43576..44994
                     /gene="dhaM"
                     /locus_tag="OQM52_001358"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1563274.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45314..47011
                     /gene="treA"
                     /locus_tag="OQM52_001359"
     CDS             45314..47011
                     /gene="treA"
                     /locus_tag="OQM52_001359"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1563275.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47090..47529)
                     /locus_tag="OQM52_001360"
                     /pseudo
     CDS             complement(47090..47529)
                     /locus_tag="OQM52_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12025 a        12329 c        11727 g        11822 t
ORIGIN      
        1 ccgaaaaaga gccagtcagt tgcttaactg actggcatta ccccttctgg ggccgttttt
       61 ttgtttaccc aaagcaactt ttccataaac cgacagcatt agccttcatc atatttgcga
      121 cgatgtataa cgcctaaaca cagggatatt gtactttaca ggtcacaagt caacgtcggt
      181 gcttaagagc cctgtgaggc gtatagcggc gttaaaaaac tgctgagaag ggtatatagc
      241 ccggaagaag tgcgtaaaac gaactgacag gataaaagtg ccctgctcac cctgtcagta
      301 aagaaattct tattaatcgt ggcgatgcct ttcctgaata gccgttaatg agccgacttg
      361 taacgcctct ttatagtgtt tacggcatac agaaacgtat cgttcattac caccaattac
      421 cacctgctca ccttcgttat aaggtctgcc tgcttgatca agacgcagca ccatgcttgc
      481 tttacggcca caaaaacaga tggtttttaa ttcaaccagt ttgtcggacc atgccagtaa
      541 gtattggctg ccaataaata attcacctcg aaaatcggta cgtaaaccat aacaaagtac
      601 gggtatatcg agttgatcga caacctccga taattcatat acttgttgtc tggttaaaaa
      661 ctggcattca tcaaccagta cgcaatgaat tgcctgctgt tcatgttccg cacgaatctc
      721 atcaaataat gatgaatttt ggttaaataa ttttgcaggc gatgacaaac ctatacgcga
      781 actgactttc ccggcaccaa agcgatcatc aatttctgcc gtatatacga cagtgcgcat
      841 gccgcgttcc tggtaattgt atgaagattg caacaatgct gtagacttac ccgcattcat
      901 tgcggaatag tagaaatata gctgtgccat cagccacagg ccctcaatga tatgaaataa
      961 agttggctgg agtttatcat aattcgtcag tttttcagct caacagtatg cacagaagtg
     1021 ggtatatgcg ttctccctta cgaagccttg cataatcctt ctgagctatc attacaactg
     1081 cctcgcttgt tataagcggg taaatgactg ctggtaacta ttcacaatct ttaacctgtt
     1141 gcgcaagtaa tagccctctg ttgacctcca ggagatagtg caatactaag tccatgctct
     1201 tattgcgact gttctacttt tcatcattcg cttaataggg aattctcgta aacacaacta
     1261 atacagaaga ctgaaaggtc gtcagcctac gataatctcc ccataaaatg tgacatgaat
     1321 caggaagttt taacctcacg tgctgcgaaa tcatcggtgt aaatagggct atatgccgcg
     1381 tcttttctgg ctaattttat gaaaagatat ttattggcgg cacaaaataa agaacaattt
     1441 tgaattcctt acattcctgg ctattgcaca actgaattta aggctctatt attacctcaa
     1501 caaaccaccc caatataagt ttgagattac tacaatgagc gaagcactta aaattctgaa
     1561 caacatccgt actcttcgtg cgcaggcaag agaatgtaca cttgaaacgc tggaagaaat
     1621 gctggaaaaa ttagaagttg ttgttaacga acgtcgcgaa gaagaaagcg cggctgctgc
     1681 tgaagttgaa gagcgcactc gtaagctgca gcaatatcgc gaaatgctga tcgctgacgg
     1741 tattgacccg aacgaactgc tgaatagcct tgctgccgtt aaatctggca ccaaagctaa
     1801 acgtgctcag cgtccggcaa aatatagcta cgttgacgaa aacggcgaaa ctaaaacctg
     1861 gactggccaa ggccgtactc cagctgtaat caaaaaagca atggatgagc aaggtaaatc
     1921 cctcgacgat ttcctgatca agcaataatc ttttgtagat tgcacttgct taaaatcccg
     1981 ccagcggcgg gattttttat tgtccggttt aagacaattt aataagaata agctataaaa
     2041 aaacggcgtc gattgctcaa cgccgtttcg tggataacac cgatacggat gttacttctt
     2101 aatgcccatc tcttcttcaa gccaggcttt aaattccgtg ccaagggtgt tatgacgaat
     2161 accgtattca acgaaggcct gcatgtaacc taatttatta ccgcagtcat ggctcttccc
     2221 tttcatatga taggcttcca ccgtttcttt ttcgatcagc atatcaattg cgtcggtgag
     2281 ctgaatttca tcaccagctc ccggaggggt ttttgccagc aacggccaaa tatccgcgct
     2341 aagtacgtaa cgacccacaa tagcgagatt agacggcgca acatccgctt tcggtttttc
     2401 taccacacca accatcggta cgctttcacc cggcgctaat tcaacgcctt tgcaatccac
     2461 aacgccatat gcggtcacat cagcaaccgg ttcaaccatg atctggctat gacccgtttc
     2521 atcaaagcgg cggatcatct ctgccaggtt atcctgtgac aaatcggatt catattcatc
     2581 cagaataaca tcaggcaaaa taacagctac cggttcatca cccactaccg ggtgagcaca
     2641 caataccgcg tgtcccaggc ctttcgccag accctgacga acttgcataa tagtcacgtg
     2701 cggtggacaa atagactgca cttcatcaag cagttgacgt tttacacgtt tttccagcat
     2761 tgcttccagt tcaaaactgg tatcaaagtg gttttcaata gagtttttag atgagtgtgt
     2821 aaccagcaca atttcagtaa tgccagccgc aatacattca ttcacgacgt attgaattaa
     2881 tggcttatcg acaagtggca gcatctcttt cgggatggct ttcgtcgccg gcaacatcct
     2941 ggttcctaat cccgcaacgg ggataacggc ttttttgact ttcgtattaa tggcagccat
     3001 ttaaattctc ctggactgtt cgtgttttga acgtgttcat atttctgtat cgcatcccag
     3061 tatatcagca ccccgaaggg gctaagttct gaaaaggagg cgagatagca taattaagac
     3121 aattacttat acgaatggcg acgatagtac caccagccga cattagcagg taatgcaaat
     3181 ttagcccgcg ttatcgtttg ctcattctgc agacaacatc aagcgcagtc gaccaccggt
     3241 tccccatatt tggcattgcc aggcatcgca tcgctggctc aattgattca aataagcgtt
     3301 acctaaagtg cctaacggaa caccattact gatttgcacc tggtgttcgc cggtatttaa
     3361 cgtcgcattc agacccgcag aaaccagaat gagatttttc agttcgcgat gataatagcc
     3421 aactaatagc ggaaactgcc ccggcagatt ggcctgacga agtaaatggt ttacctgctt
     3481 caataacgcg cccaactctg gcaaccgttg attttggtgt gcaagctgtt cctgtaataa
     3541 tccgttaaac aatgcgcgta ataacaaggc agcaagtacg ccattatgtc cagctcgggt
     3601 gacatcaagg caataaaatg ccagatcgtt ttccgaaagt gcggcaatat caagcaccag
     3661 gccgggtttg tccgcggcaa ccaattgacg gtaattaacc cggcaatggg aaatcacctg
     3721 ctgaaccggc ggttgtagtt cctgtaataa tttcgccgct gcggcagggt tatcaaccat
     3781 tgcatcccag tcgcgaaaaa gcctttcctc ttcctcaacg cgcgaattaa acatgctggg
     3841 atagagacag gcaaaaacca tctcgcgcaa gcgattcaga tctttaactg gtttcagcaa
     3901 aacatcttca acgcccagac gtaacgcttt ggcaatatct gccatatttt cagtggcaga
     3961 tatcaccaga actggggtct ggtcgcctct gttacgtata tgctccagca gtttaagccc
     4021 gttcattcgt ggcatcgcga tatcacatat catcaggtct ggagtgaaac ctcccagcaa
     4081 ctcaagggca tccaccccat cagccgccag taccgttgtc gctcccaatg aggaaaacca
     4141 tgaatccaga agcgagcgaa atacctgctc atcttcaaca atgagaatct gttttccgac
     4201 caatggctgc gtcatgttct ctcccctgac tggctttact caatagtggc atgctattgc
     4261 cacctgcgcc tgtcagaatt aacttaagtg tagataaaaa ttctggtcaa atgttggtgc
     4321 gtaccaacgg caaaagttcg tccattttcc tttccactgc cagctgtccg gccgcaatgg
     4381 cagcgtgcgc gcgatggaaa tcaagcgtag atatttgcgg gcaaacaggt tgaatcagaa
     4441 tatcgggcgg atcacctgcc atgcggttcc ttttaaggcg gttctccaac acctggatag
     4501 acgtggtcat aatctctgtt gccgttggcg ctgtcacagc ccgacgtgtc gttatgctgc
     4561 ctaacctttc tttcagacgc gcatgccacg gtagagaatc accattctcg ctattttctt
     4621 cactgacatt aaaggagagc agatcttgtt gcatcaaatg agcatcgtgc tgcaggtcga
     4681 ccgctatcac aatatcagcc cccaatgcac gcgtaagaga aataggaatt gggttaacta
     4741 ctgccccatc aaccagccag tagccgttat gtgcaacggg tgccatgagt cctggaatac
     4801 tgcatgatgc gcgaatagca agatggagat cgccttcagt aaaccataat tcacgccccg
     4861 tacttaaatt ggtggcaaca gccgcaaagc gacgggaaca attttcgatc tctgtttccg
     4921 gcattatttc gcgatattga ttgaagacac gctcgccgcg cagtaaccca ccgcgctgcc
     4981 aggagaggtc catcaggcgt aaaacgtccc aataactgaa agaggttacc caatcttcca
     5041 gcgcagataa tcgatcgcat gcataggccg cgcccaccag cgaaccaatt gaacatcctg
     5101 caacgatatc aatttcaata cccacttttt ttagcgcatt aataacgcca atatgcgacc
     5161 aacctctcgc cgcgccagat cccagcgcca gccctatttt tatctttctc attattcccg
     5221 caagattccc ctggaatgca atcgtagcca catcgctact gctcggttaa catagtgcta
     5281 ctcaggcggt taatacgccg ttattgtttc cagggagatt atttgtgtct cagctttgtc
     5341 cctgtggtag tgctgtcgag tatagcctat gttgccaccc ttatgtgtct ggtgaaaagg
     5401 ttgcacctga tccagaacat ctcatgcgtt cgcgttactg cgcttttgtg atgcaagacg
     5461 ctgattattt aataaagacc tggcatccct cttgtggagc agcggcgtta cgtgccgaat
     5521 tgatgaccgg atttgcgcat actgaatggt tgggattgac cgtatttgaa cattgttggc
     5581 aggacgccga caatatcgga tttgtcagtt ttgttgctcg atttaccgaa ggtggcaaaa
     5641 caggtgcaat aattgaacgt tcgcgatttt tgaaagaaaa cggtcaatgg tactatattg
     5701 acggtacacg tccgcagttt ggtcgcaacg atccctgccc ttgtggttca ggtaaaaaat
     5761 ttaaaaagtg ctgcggccaa taatggttga cggtacggtt tagcaaacac tctcaacaag
     5821 gtttttccag caatgcattc actccaacgt aaagttctgc gtactatttg tccggaccaa
     5881 aaaggtctga tcgcacgtat taccaatatt tgctacaagc acgagttaaa tatcgtacag
     5941 aacaatgaat ttgttgatca ccgtaccggg cgctttttta tgcgcacgga actggaaggg
     6001 atttttaatg attccaccct gctggcggat ctcgatagcg cattgccaga aggctccgtg
     6061 cgtgagctga atcctgccgg tcgtcgccgg atagtgattc tggtcactaa agaagcgcat
     6121 tgccttggcg atttgttgat gaaagccaat tacggcggtc tggatgtcga aatcgcggca
     6181 gttattggta accacgatac tttacgttct ctggttgagc gttttgatat tccgtttgag
     6241 ctggtaagcc atgaagggtt aagccgcaac gagcacgatc aaaagatggc ggacgccatt
     6301 gatgcttatc aacctgacta tgtggtgctg gcgaagtata tgcgggtatt aacgccggaa
     6361 tttgtggcac gcttcccgaa taaaatcatc aatattcacc attcattcct gcctgcgttt
     6421 atcggcgcac gtccttatca ccaggcctat gaacgtggtg tgaagattat tggcgcaacc
     6481 gctcactatg tgaatgacaa tctggacgaa ggcccaatca tcatgcagga cgttattcat
     6541 gtcgatcata cctacacagc tgaagatatg atgcgcgcag gtcgtgacgt cgagaaaaac
     6601 gtcttaagtc gtgcgctata caaagtactg gcgcaacgcg tctttgttta cggtaatcgg
     6661 acgattattc tttaatcgcc agcaaaaata actggttacc tttaatccgt tacggatgaa
     6721 aattacgcaa ccagttcatt tttctcaacg taacacttta cagcggcgcg tcatttgata
     6781 tgatgcgccc cgcttcccga taagggagca ggctagtaaa aagcattacc ccgtggtggg
     6841 gttcccgagc ggccaaaggg agcagactgt aaatctgccg tcatcgactt cgaaggttcg
     6901 aatccttccc ccaccaccat cactttcaaa agccccggaa ttctcaaacg aatctgcaat
     6961 caaatattct gcccaagcgg ggaaggatga gaagcttcga ccaaggttcg acttgagcgc
     7021 cagcgagaga gcgttgccgc aggcaacgac ccgaagggcg aagcgcgcag cgctgagtaa
     7081 tccttccccc accaccatcg cttccttgaa taatcaaaac ctgttactcc gagctaattt
     7141 tgcccgatgg caccacgttt accgggccta caatatcctc ccctgtaggt cggataagcg
     7201 aagcgcatcc gacaactcac cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt
     7261 ctatgtgaac taaaattcgt ttagtgacga gcgcgcacca gctggtactt acgtgtcaga
     7321 tactccaccg gtacgctcca gatgtgtacc agacgcgaga acgggaacag caggaataac
     7381 gtcatcccca gcaccaggtg cagacggaag ataaacgcca caccatcgag gtgttgagaa
     7441 gcgccaccgt ggaaggtcac caccgactgc gcccagccaa ccagtttcat catctcgcta
     7501 ccgtccatat gctgagcgga gaacggaatg gtcagcaggc ccagcgcgca ctggataacg
     7561 agcagcgaca ggatcaggat atccgctccg gtagtggttg cacgcacgcg tgggctgaac
     7621 atacgacgtt tcagcaaaag cacgccgcca atcagacaca gcacgccgct ggcaccacca
     7681 gcaaacatcg ccattttctg tttcacttca atcggcagcc aggcttcata catccagtgc
     7741 ggcgtcaaca taccgaagaa gtgaccgaca aaaatcccca gaataccgat atggaacagg
     7801 ttcgacgcca ggttcatccc tttgcgatcc agcatctggc tggacgccgc gcgccaggtg
     7861 tactgcccgt agtcataacg cagccagcta ccaatcagga agaccgcccc ggctatgtac
     7921 gggtagatat caaagaagaa catattcagg aattgcatta gtgctgtcct ccggtggtga
     7981 tattcagata ttgcggcgcg acggcaccgg caaagcgacg ctgatgagca gtgattgctg
     8041 aatcaccgca gcctttgtca gcaaagaatt taacctgctc ttcttcccag acagcatcca
     8101 gcgcctgcgg cgtatcatcg cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt
     8161 cgatagcggt attggccaat ttcagcagca gatcaaacat cacggcataa cggctttcac
     8221 gctgttgcag acgcgcgctc agcaatgcca gaatcggcgc gatatctttc aaaccttcca
     8281 cggcttcgct ttgcggcagc tgcgacaggt actccagata cagcggcaga tggtccggta
     8341 attcgcggct gtttaactgc aagccgtgct gctcgtactg cgccagcagg tccaccatcg
     8401 cctggccgcg gtcacgggat tcaccgtgaa catgttcaaa tagcaacagt gacgtggcac
     8461 ggccacggtc gaacagttcg ctgtactgga cctgggcatc gagcggatcc atcgccgtta
     8521 aatcgcgcag gaaaatgccc agcgcatggg catcttcttt cgacagattt ttcgacgcag
     8581 caatcgcctc aaacatctct tgttgatgct gccataaggc agcatccgga tattcaagga
     8641 gacgcgatac aatcacgagt tcgatcatgg atgcggctcc gttttgctgg tcacatcgat
     8701 ggcatcgata cgacggctgt tgaacagatt gaatttggta tctgagccgt ggcagccatc
     8761 accaaaggta aagccgcagc catttttctc cgggaaggct tcccgtgcca gttcacgatg
     8821 actactcggc accacaaagc gatcttcgta gttagcaatc gccagataac ggtacatctc
     8881 ctgtgcctgg gcttcggtca gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc
     8941 aacagtttca gcacgtttgt aatgacgcat cgccagcata cgtttcagcg cacgcagtac
     9001 cggtttggta tcaccggcgg tcagcagatt cgccagatac tgtaccggaa tacgcaagct
     9061 ttcgacgtct ggcagaattc cgttgctgcc cagctcgccc gcgtctgctg cagactgaat
     9121 cggagacaga ggcggcacgt accagaccat cggcagtgtg cgatattctg gatgcagcgg
     9181 cagcgccagt ttccattcca tcgccatttt ataaaccggc gactgctgtg cagcttcaat
     9241 aacgctcagc ggaataccgt ctttaatcgc ctgctcgatg actttcggat cgttcggatc
     9301 gaggaacacg tccagctgac gctggtagag atctttctcg ttctcggtgc tggctgcacg
     9361 ttcaatcgcg tcggcatcgt acagcagcac gccaagataa cggatacgac cgacacaggt
     9421 ttctgagcac acggtcggct gacccgcttc aatacgcgga tagcagaaga tgcacttctc
     9481 agacttaccg ctcttccagt tgaagtagat ttttttgtac gggcatccgg tgatgcacat
     9541 acgccagcca cggcatttat cctggtcgat caggacgatg ccatcttctt cacgcttgta
     9601 aatcgcaccg ctcgggcagg tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg
     9661 cggcaaatac atcataaagg tgttttcgaa ctggctatac atcgccttct ggatgttgtc
     9721 gaagttcttg tctttcgcca gtttatcaaa ctcaccgccc aggtcatctt cccagttcgg
     9781 ccctttttcg attttcgcca tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg
     9841 ctgcgatttg ctgccttccg gcgcggtatg caggttctga taatcaaaat cgaacggctc
     9901 gtaataatcg tcgatccccg gcagatgcgg gttagcaaag attttaccca gcagcatggc
     9961 tcggttaccc atgcgcggtt gcagtttgcc attgatttta cggatccagc cgcctttgta
    10021 tttttcctgg ttttcccagt cagtcgggaa gccctggccc ggcttggttt ccacgttgtt
    10081 gaaccacgcg tattccacgc cttcacggct ggtccagacg tttttacagg taactgaaca
    10141 ggtgtggcag ccgatgcact tatcgagatt cagcaccatg ccgacttgtg aacgaatttt
    10201 cattttacgc tctcctgtac ctggtcattg ccttcgccat ctaaccagtc aatgttcttc
    10261 atcttacgca ctacaacaaa ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag
    10321 ccgtatgcca gatgggcata gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg
    10381 accgagttat ggataccacc acgctgttgg gtaatttccg aaccaggcag gttaacgata
    10441 cgttcctgcg cgtggtacat catggtcatc cctgccggaa cacgctggct gacaaccgca
    10501 cgggcagtca gtgcaccgtt gctgttgaag acttcaatcc agtcgttatc ggcgataccc
    10561 agatctttgg catcggcttc actcaaccag accaccggac caccgcgacc taaagtcagc
    10621 atcagcaggt tgtcgctgta ggtggagtgg ataccccact tctggtgcgg cgtcaggaag
    10681 ttgagcgctt tttccgggtt gccgttggat ttctggccta tcacttcttt caccgaacgg
    10741 gtgtcgatcg gcggacgata aaccagcagg ctttcaccga aatcacgcat ccactggtga
    10801 tcctgataca gttgctgacg accagagagc gtacgccatg ggatcagctc gtgaacgttg
    10861 gtgtaaccgg cgttgtaaga aacgtgttca tcttccagac cagaccaggt cgggctggag
    10921 ataattttgc gcggctgtgc ctgaatatcg cggaagcgga tcttctcgtc ttctttattc
    10981 agcgccagat gcgtatggtc acgaccggta aattcgctca gggcagccca ggctttcacg
    11041 gctacctgac cgttggtttc cggtgccagc gtcaggatca tctctgccgc atcaattgcg
    11101 gtgttcagca tcggctggcc ttttgccgga ccttccgctt tggtgtagtt gagcttacgc
    11161 agcagatcca tctcgctctg ggtgttccag gcaatccctt taccgccgtt accgattttc
    11221 tccatcagcg ggccgataga ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg
    11281 accataatgt gcggtgcagt tttgcctggg atcaggtcgc attcgccttt tttccagtct
    11341 ttcacatcca gcggctgcgc cagttcagcg gcagagtcgt gctggatagg cagcgtgacg
    11401 atgtcggttt ctttacccag atagccgacg cacacttcgg agaatttctt cgcgatggct
    11461 ttgtagattt cccagtcgct tttcgcttcc caggccggat cgaccgccgc agacagcggg
    11521 tgaataaacg gatgcatatc cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc
    11581 ggcaaaatga tgtcggaata gagacaggtg ctcgacagac ggaagtccag cgtaaccacc
    11641 agatccagct tgccttccag accattgtcc tgccagtcca cttcttccgg cttcacgccg
    11701 ccctgttgcc ccagatcttt accctggata ccgtgctccg tccccagcag gtacttgagc
    11761 ataaactcat gacctttacc ggaagaaccg agcaggttag aacgccagat gaacaggttg
    11821 cgcgggtggt ttttaccgtt ttctggttgt tctgccgcaa aacggatgga accctctttc
    11881 agggatttca ccgtatagtc caccggattc atcccggctt tttccgcttc gccagcgata
    11941 gtcagcgggt tagtgcctaa ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg
    12001 acgttaaagt cgatcaagtg tccggtatag cgggatttgt ccgccatcgg tgacagcaac
    12061 tcttccgccg tgacggtttc ataacgccac tggctggagt ggttatagaa ataagaagtg
    12121 ctgttcatgt gacgcgccgg acgctgccag tcaagggcaa acgccagcgg ctgccagccg
    12181 gtttgcggac gcagtttttc ctgacctaca tagtgcgccc agccgccccc gctctgaccg
    12241 acacagccgc agaaaatcag catgttgatc agaccacgat agttcatatc gaggtgatac
    12301 cagtggttca gccccgcacc gacgataatc atcgaacgac cgtgcgtttt atcagcgtta
    12361 tcggcaaatt cacgggcgat gcgaataatc tggctgcgag aaacgccggt aatctgctcg
    12421 gcccaggccg gggtataagc tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc
    12481 aggccacgtt ccagaccgta gtttgccagc gtcagatcat aaacggtggt caccagggcg
    12541 gtgctgccat cagccagttg caggcgtttc accggcagtt tgtgcagcag cacgttttcc
    12601 agttccactt tgttgaagtg ttccgtgccg tcgccaccaa agtacgggaa gcctacctca
    12661 gcgatctcat cctggctacc cagcaggctc agttgcagct cggtttcttc gccagttttg
    12721 ccgtcgcgct gctcaagatt ccatttgccc ttctcgcccc agcggaagcc aatagaaccg
    12781 ttcggcgcaa ccatttcgcc attggtatta aaggcgacag ttttccattc cggattgttt
    12841 tcctggccca gcgcatcaac cagatcagca gcgcgcagca tacgacctgc agcgtagtaa
    12901 ccgtcgcgtt cttccagcat caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg
    12961 gtgaaatact ggcttgggtt gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc
    13021 agcgccattg ccgcatcggt gccctgtttc ggtgccagcc acagatcgca cagtttggcg
    13081 atttcagcgt agtctggtgt gacggcaaca gttttggtgc ctttgtaacg cacttcagta
    13141 aagaagtgag catccggggt acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag
    13201 ctggagttat accagtcagc agattccggt acgtcagttt gctcgcccca ggtttgcgga
    13261 gacgcaggag gcaggtcgca gtaccagtcg tagaagctta agcaagtacc gccaatcagc
    13321 gagagatagc gtgcacccga tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa
    13381 ccagcaacac ggtccgggcc gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt
    13441 tcgttcacct cctgccaaga agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag
    13501 cttttcgctt tatcggcgtc ttcaatgata gaagcccatg cctcaaccgg atcgctatgc
    13561 agcgccttcg cttcacgcca cattttcatc aggcgtttgc gcatcatcgg gtatttcagg
    13621 cggttggcac tgtaaagata ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca
    13681 tggtttggca gatccggacg ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc
    13741 agaccgtttt tgacgtagat tttccagctg caggagccgg tgcagtttac cccgtgggta
    13801 gagcggacga ttttgtcatg ctgccaacgc tggcgatatc catcctccca gtcacggttg
    13861 gtattgagaa gctggccatg cccatcggca aaggtttcac ccttctgttt gaagtagcga
    13921 aaccggtcca ggaatttact catcggtttt ctcctgtggg agcctgtcgg ccctctgata
    13981 aatcgacatt gctaatgttt aagggcaagg taacgctctg aaacagcgtg ggaattgata
    14041 acgatcaaga gtgatgggga tgaaaaataa agtaaattcc taagataccc ctttaggata
    14101 attaacagtt acagcataac atgctgtaaa ttaagacgtt ttatacctat agttatttaa
    14161 actattaaga tttttatgca aatgggtatt aaggagtatt ccccatgggt aacatattaa
    14221 tggctcttaa tgaaatccgg cgaaataagc catataacac taatatgttg taactaaatg
    14281 acgcataaaa aaagcgcggc atcatgccgc gcaaaggata atcaaatatt aagatttatt
    14341 ttttagaatg ccgaccatat accgcccaag taatcactac gcaggcgata tagaaaatca
    14401 aaaatacttt cattgcacct actggcgaac ccgttaatgc cagcgagcta ccaaacgctt
    14461 tcgggataaa gaagccacca atcgcgccaa tcgcagagat gaaacccagc gccgctgccg
    14521 tgccggttgc cgcttcacgc atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat
    14581 ccattgtcag tttacggaag atcactgaaa tcatctggaa agtggaacca ctacccagcc
    14641 cagctgtcag gaacagcgcc aggaagaccg cgaagaacgc catgaagctt ccgccctgcc
    14701 cgtcagtcgg taaggtcagg aacagcaggc cgctgaaaat cgccatcaga ataaagttca
    14761 ccagcgtgac acgagttccg cccagacggt cagataatgc accacctgca gaacgcgcca
    14821 gcgcaccaat aaacggcccg aagaaagcgt attgcagaat ctgaacatcc gggaactgcg
    14881 tttttgacag catcgcaaag cccgcggaga agccgatgaa ggagccgaag gttgccagat
    14941 acagcaggct cataatccac agatgacccc gtttgagtac cggcaactgc tccttgatgg
    15001 aggctttcga ggtagcaaga tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa
    15061 ggaacggcac ccatatccag gacgcattcg ccagatacag ctcagtccca tccggctgtt
    15121 tgacgccctg gctaccaaat actgcgaaaa tcgacagtga taccaccagc ggagcaacca
    15181 actgcatgac gctgacgccc atgtttccca gaccaccatt cagacccagc gcgccaccct
    15241 gcttctgttt cggaaagaag aagctgatgt ttgccatact ggatgcgaag ttcgcgccag
    15301 caaagccgca tagcagagag atgatgatga agacgctata aggcgtggag gtatcctgca
    15361 cggcaaaacc cagccagacg caaggaataa tcagaatacc ggtgctgaac gccgtccagc
    15421 gacgaccacc gaagatagga accataaagg agtatggaac acgtaataac gcgccagaaa
    15481 ccgaaggcag cgcagtcaac ataaatagct gatcggtcgt aaaattaaaa ccgactttcg
    15541 gtaagttcac cgcaacagcg ctgaacaaca tccatacgca aaacgccagc agcagacagg
    15601 gaacggaaat ccacaggttg cggctggcaa tacgttgacc gcgttgttgc cagaacgcag
    15661 gatcttccgg tcgccaatct gttatgacag ctccagtagc cctttcggag gcagatgagt
    15721 gactcataga cacctctgat actcgtttcg atgtctgcca ccttagtgtc tggagctaaa
    15781 ggcaatttga tgtaaatcaa acgataagat aactttatct atcattgata tttatcatta
    15841 cccatagtga gtacagtgat ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc
    15901 cctaccctta ccgatgtaaa gcgactaacc acacggcaaa taaggagtaa ctctttccgg
    15961 ctatgggtat acttcagcca atagccgaga atactgccat tccagaatat atcgtcacat
    16021 tcattaaggt tattgctcat ttaaagcctg aaggaagggg tttacatgct taaacgttgt
    16081 ctctctccgc tcaccctggt taatcaggtt gcgcttattg tgttgctttc tactgctatt
    16141 ggactggcag ggatggcggt ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat
    16201 gcgatcaaca aagcgggatc gctgcgcatg caaagttacc gtctgttggc ggcagtgcca
    16261 ttaagcgaga aagacaagcc cttaattaaa gagatggaac aaacggcatt tagcgccgag
    16321 ttgactcgag cagcagaacg agacggacaa ctggcgcaat tacagggctt acaagattac
    16381 tggcgtaatg aactgatccc tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg
    16441 gatgtcagcc agtttgttgc cgggcttgat cagctggtat ctggttttga ccgcaccacg
    16501 gaaatgcgta ttgagacagt ggtactggtc catcgggtaa tggcggtatt tatggcactt
    16561 ttactggtgt tcactattat ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg
    16621 ctggcaatgg cgagtgccgt cagccatcgc gattttaccc aacgcgcaaa catcagcggg
    16681 cgcaacgaaa tggcgatgct tggaactgcg ttgaacaata tgtctgcaga actggccgaa
    16741 agttatgccg tacttgagca gcgggttcag gagaaaaccg ccgggctgga gcataaaaat
    16801 cagatcctct cttttttatg gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt
    16861 gaacgcctgt caccggtact caacggctta cagaatttaa ccctgctacg tgatatcgaa
    16921 ctgcgggtgt atgacactga tgatgaagag aatcatcagg agtttacctg ccagccagat
    16981 atgacttgtg atgataaagg ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat
    17041 cgcggcacga ccctgaagtg gcggctggct gactctcata cgcagtacgg tattttgctg
    17101 gcgaccctgc cgcaggggcg tcatcttagc catgaccaac aacaactggt ggataccctg
    17161 gttgaacaac ttaccgccac gctggcgctg gatcgccatc aggaacgtca gcaacagttg
    17221 atcgtgatgg aagagcgtgc caccattgcg cgcgaactgc atgattctat tgcccaatct
    17281 ctctcttgca tgaagatgca ggtgagttgt ttacagatgc agggcgatgc gctgccagaa
    17341 agcagccgcg aactgttaag tcagatccgt aacgaactga atgcatcctg ggcgcagttg
    17401 cgtgaattgc tcaccacatt ccgcttgcag ctcaccgagc ctggattacg tccggcgctg
    17461 gaagcgagtt gcgaagagta cagcgccaaa tttggcttcc cggtgaagct ggattatcaa
    17521 ttgccgcctc gtctggtgcc ttcacatcag gcaatccact tgttgcaaat tgcccgtgag
    17581 gcattaagta acgccctcaa acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa
    17641 aacgataatc aggtcaaact gaccgtccag gataacggct gtggcgtgcc tgaaaatgcc
    17701 atccgcagca atcactacgg catgataata atgcgcgacc gtgcgcaaag tttacgaggc
    17761 gattgccgcg tccgccgtcg tgaatcaggt ggcaccgaag tggtggtcac ctttattccc
    17821 gaaaaaactt tcacagacgt ccaaggagat acccatgagt aatcaggaac cggctactat
    17881 cctgctgatt gacgatcacc cgatgctgcg aactggcgta aaacagctta tcagtatggc
    17941 accagatatc accgtggttg gcgaagcgag taatggcgaa cagggtattg aactggcgga
    18001 atctcttgat cccgatctga tcctgttaga tctcaatatg cccggcatga acggtctgga
    18061 aacgctggat aaactgcgcg aaaagtccct ctcagggcgc attgtggtat tcagcgtctc
    18121 taaccatgaa gaagatgtgg tcaccgcact gaaacgcggc gcggatggct atctgttaaa
    18181 agatatggaa ccggaagatc tgctgaaagc attgcatcag gcagctgctg gcgaaatggt
    18241 attaagcgaa gcattaacgc ctgttctggc cgccagcttg cgcgctaacc gtgccactac
    18301 tgagcgcgat gttaaccagt taaccccacg cgagcgcgat attctcaagc tgattgccca
    18361 gggtttgccg aacaagatga ttgcccgccg cctggatatc accgaaagca cagtaaaagt
    18421 gcacgtcaag cacatgctga agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg
    18481 ggtgcatcag gagcgcattt tctgattacg gttcccagcg cagttcgtcg tttgacaatg
    18541 catcgaacgg ttcgacaagc gttagcgtga tctcattgga ggagacacgc tgcccctggt
    18601 tatcttccac caccacagac aatcgccagt gattgcttgc cccttccccg ttctgccagt
    18661 caggcatgat cagcgtccag ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta
    18721 atatctgcgt atcgccctgc caaatcagtt gccgaatacc gtaacgactg cggatttgta
    18781 atttcagcgg cactgtttcg ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg
    18841 ttaacgtttt tcgctgtcgg tactcaagag tcggtagatt atttcgctgc ggattgtcat
    18901 agcgactacc acgtaacgac tgactttcgg caacctcgcc cgcagaaagt tgttttttga
    18961 gcggtacacc aaagcggtaa ttaagattca gcccgaggtt attttgattt tcgccacttt
    19021 ccccctgttt atgctgggcc gtcacagtga ctaatggcac aggggtgtaa tttaatccca
    19081 gactcaacgc gacgggattg tgataacccg taccagagtt aaacaaatca acacgatcac
    19141 caaaatactg ttctaggctg acactggtat tgaggtgttg atagaacggc atgcgcatcc
    19201 gagctgtcag gtcgtacccg cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc
    19261 atgcagcaaa cggctgataa aagtttgccg ataatcgcaa atattcgccc cacgcttcgg
    19321 caccaaagcc cgctcgctga agattttcgt cctgcaagtt gtcataaaaa gtgttataac
    19381 ccaccagcca gttgccgcgc gcccagcgtt gcccaacgcc cacattgctc accaacccat
    19441 tatcctgctg agtaagacca agctggctcc aggtgagata acgatcatta tcttgtaacg
    19501 gcacaaacca gcttccacga ctgccggtga aatgtccttc gttatccact ttgacgtcaa
    19561 cactggcatt tccccacggt gatagccagg actctacgtg ctgattaacc tgttgactaa
    19621 gcgcgtcgcg gacttttccc aatgcgaaag cttttgcctg ctcgccagta tccagcccgt
    19681 tatcattcat actggtttcg ccgaaatctt tcacaatttc agcaaagtgt ttttccccat
    19741 catgattttc gggagccatg cctaaatccg gcagaccatc attgttatta tcaaagggat
    19801 ttgccgcttt ttgctcgaag gtagattgtg cgttagctgt accgcctgct acaagcaaga
    19861 gtaaataaaa cgggataatg cgaggaacga aacggctcaa aacgtcggta acttccggta
    19921 ataatgaaaa accatagcct taaagaataa cgattactgg caacttttgt cgtattttct
    19981 cgcgctttca ggaaaatcct taaaaatgtg accgattggt cagacgctgc agaagcagta
    20041 agatagaaga tgagtgtcta attccctgaa tacaggagta aataatgcaa aaaatagtga
    20101 tcgttgccaa tggcgcacct tacgggagcg aatccttgtt taacagcttg cggctggcca
    20161 ttgcgttacg agagcaggag agcaatctgg atctgcgtct gttcctgatg tctgatgcgg
    20221 tcacagccgg gttgcgcggg caaaaaccag gggaaggcta caacattcag caaatgctgg
    20281 agatccttac cgctcagaat gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg
    20341 ggattagcac acttcctctg attgatgggg tggaaatcgg tactcttgtg gaactggcgc
    20401 aatggacgct gtcagccgat aaagtgctca cattttaatg tttaaggggg cgtctgacgc
    20461 cccactatga gggaagttta ctcaggcgaa tcccggcctt aacacaccat ccggataatt
    20521 ctccgccagt agttttttta ccgataccag caaatcattc agcccatcgt cctgcattcc
    20581 cagtttgata agctcctgtt ccagtgaaaa caggtattgt gcattggttc ccagcggacc
    20641 gctcgccgcc gcaatcaacg gcgcgatgac ctgagcgcga gtgtcagatt catattctgg
    20701 atgtcgcggg tccataataa acacaatggc gtttactgtg cgtccatcat caagatcaag
    20761 ctgacaccag gttggcagat aacagccggt aatcatctcg cgcttccaca acagggttag
    20821 ttcctgctcc agcgtctctt ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc
    20881 tttcagtgca agcatccgtc ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag
    20941 gcaaaacgcg cgatgccatc caaccagtgt accggtgcat gattcggtaa actccagtgc
    21001 cggattccac atcaacgatc cataaccgaa gatccacacc ggtccctcat caggtcgaca
    21061 agccagcgtc gccgccagcg aagccgcccg ttgttctgct gaccataaga gtgattcttc
    21121 aatggcacca aatgccgttt tacaatcggc attcatcaag aaatcacgcg ttatcacctt
    21181 acacctccgc cactgcatac ttccctgcga caactttttg gtgtcttccg gacattttta
    21241 ccgctcattt tgaaaccact aacggaacaa tatgcaatcc acggaccact tgcaaggcaa
    21301 caccgcacga atagctttaa ctaagctacc ggttttacga ttttttatgc catttatcat
    21361 catccccttt ggcatattca tgcttcacag cagcccaggc cactttatgc gcggtttctt
    21421 cgcgactggc gtcatcacgc cgatcttctt tatctttata ttgatcccat gcgctgttga
    21481 acgcttcttt atagatatcc tgggcatgag acggtagaac gtgctttacg ctttccggca
    21541 gatcgctttt cgttttatac ggcatcgttg actcctttta caaagttgaa cattaagtgt
    21601 ggtcaacaat atctactctt gccaaaacat aagaattttc atgtcagact gttcttaaga
    21661 aacgctaaaa caaacattgt ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa
    21721 atcgacagcg gggtaaaaat tcgtaaaatg ttaccgatat ccagacactt tctgttattt
    21781 ttcctaccta ttttttacat tttcgataaa ttcatctgca aagggataac cataatgtca
    21841 aatgctcaag aggcggtaaa aacccgccac aaggagactt cgcttatttt cccggttctg
    21901 gtgctggtag tgctgttcct gtggggaagt agccagacac taccagtggt cattgccatc
    21961 aatcttcttg cgcttattgg tattttaagt agcgccttta gtgttgtccg tcatgcggac
    22021 gtattagccc atcgcctggg agaaccttac ggttcgctta ttcttagcct ttcagtggtt
    22081 attcttgaag tcagtttgat ttcagcttta atggcaaccg gcgacgccgc gccaacgcta
    22141 atgcgtgata cgctctattc aatcattatg attgttaccg gtgggctggt tggcttttca
    22201 ttattgttgg gcggtcgtaa gtttgccacc caatatatga atctgtttgg tatcaagcag
    22261 tatttaattg cgctgttccc cctggcgata atcgtactgg tatttccaat ggctctgcct
    22321 gcggcgaatt tttcaaccgg tcaggcgtta ctggtagcat taatttctgc ggcaatgtat
    22381 ggcgtatttt tgctgatcca ggccaaaacg catcaaagtt tgtttgtcta cgagcacgaa
    22441 gatgacagtg atgacgacga cccgcaccac ggtaaaacgt ctgcccatag cagcctgtgg
    22501 catgctatct ggttgattat ccatctgatt gccgttattg cagtcaccaa aatgaacgcc
    22561 agctcgctgg agacattgct cgacagcatg aatgcccctg ttgcctttac tggcttcctg
    22621 gtggcactgt tgattctgtc gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac
    22681 caggttcagc gcgcgatgaa tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc
    22741 gtacctgtcg tcacgctaat tgcctttatg acgggtaacg aattgcagtt tgcacttggt
    22801 gcgccagaaa tggtggtgat ggtggcctct ttagtgctgt gccatatctc cttctccacc
    22861 ggacgtacta acgtgctcaa tggcgcagcg catctggcac tgtttgccgc ctatttgatg
    22921 acgatatttg cctgacagtt atatctcctc cgatgacgga aatcgggaga gataagataa
    22981 attcctgctt attgtatttc ttcatactac ctggacagct cgttaatcag aattgcgagc
    23041 ctgtaccaca acctgcacga aaccactgca acagctgttg aaaagccgga atgtactctt
    23101 tattgcaaac atttcaggga tgaagtgaag atgaattgag acgtagtatc cacaccaagc
    23161 cggaattatc cggtgaggcg caatgttgcg ggggctttat ccctggtggc attggttgct
    23221 ggaaagagaa aacccccgca cgttgagagg taaaacctga caacaaaacg ggggctaatc
    23281 ttgaaaccag acacttcaag aatagccgca aaatgttgtc attacaacaa ggcggctata
    23341 tgacgctcgc gcagtttgcc atgactttct ggcacgacct ggcagcaccg atcctggcgg
    23401 ggattattgc cgcagcgatt gtcagctggt ggcgtaaccg gaagtaaggt gaaaacgggc
    23461 gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg
    23521 ccttttttgt atctggcacc catgaaaaaa gtcttaacgc agaacgctaa gactttattt
    23581 ttcaagcaaa aaagattact tgctggtatc cagttcttcg aaacctttca ccagatcatc
    23641 aatcgctttc atctgcttga ggaacggttc cagtttagcc agcggcagcg cggatggacc
    23701 atcacatttc gcatgttccg gatccggatg cgcttcaata aacagccccg ccagacctac
    23761 cgccataccg gctcgtgcca gctcagccac ctgagcacga cgaccaccgg aagctgcgcc
    23821 aaacggatcg cggcattgca gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga
    23881 cactttcttc ataatgctga agcccagcat atcgacaacc aggttgtcat agccgaagtt
    23941 agcaccacga tcgcaaagaa tcactttttc gttgccgcct tctttgaatt tatcaacgat
    24001 attacccatc tgtcccgggc tgacaaactg tggtttcttg acgttaatta ccgcaccggt
    24061 tttcgccatg gcttcaacca ggtcagtctg gcgagcaaga aacgccggca actgaatcac
    24121 atccacgaca tcagcaacgg gctgtgcctg acttggttcg tgaacgtcgg tgataatttt
    24181 cacgccaaaa gtctgcttca actcctggaa gattttcatc ccttcttcca ggcccggtcc
    24241 acgataagag tggatggagg agcggttggc tttgtcaaaa gaggctttga acacgtaagg
    24301 gatacccagt ttctgggtca cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg
    24361 agattccagc acgttcatac cgccaaacag tacgaacggc aggtcatttg ctacgttgat
    24421 gtcgccaatg ctaaccactt tttgtttcat aggatcgcct taatcttgag taaaatgtcg
    24481 attaattaat gcagcacaat atgtttatgc gcgatgttat ttatttgcgc acggatcatt
    24541 tcgctgatcg ggtcttccgg acaccgttca acgaaataac ttaaatcgtt caacgcaacg
    24601 tgttcgcaat ccagttgcgc ataaatcaac ccacgatcgc gaatttcata gggatcttca
    24661 gggttgaatt gtaataaagc ttcgctggtg cgtaacgcca gctccatctg attttcttcc
    24721 atcaacgagg ctttgagtgt atccagcaat ttgcggatta cctcaatgtt atcagcttca
    24781 tcaaggtctt cataaaacag ttccgccggc gggctgatat ttccctttaa ccatccgtcc
    24841 agcatatgtt cgcttaacga ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc
    24901 ggacattcaa tgcgcaatat cagctgcgta gggaaaatca ccggcagcag cggcaaatcg
    24961 agacgattcg cgacccataa taaaaccgca cctaatgata ccgcactgcc ctgtcgattc
    25021 tttaacacct ggtccagcca taatgcatcg gaaagacgat aaacgccgcg tgaggcttta
    25081 aatccccagt cgccgtaaaa cagcgcgatc agtttttcca gctgctcttc taaaggcaga
    25141 agctggctga tttcttcctt cgccagacta acgagacgct ccagttcgtc gtaaacatct
    25201 tgcgagggaa aatcgcggcg gattgcttcg caagccagga tcatgccttc gcacaatggc
    25261 gctttattaa attcgaaatc agctaacgat ctcatgactt accccagtaa cggtactttt
    25321 gtggtggcga gtttaatgat gatgtacagc accaccaacg ccagcgggaa ggcaataatg
    25381 cgcgcctgct gactgtgcat acgacgataa tcgagtgcaa taaaacccaa aacgatataa
    25441 atgataactc caaacagctt ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg
    25501 tgcgctttag ccatcaacgc aatgccgctt aacaataaca ccgtatcgac aaccggcggc
    25561 acaatgcgcg tccagcgagc aaatgcctgt ggatgcttct gataacgtag ccagaaacgt
    25621 aaggttaata gcccaacaga aagcgcgata ctaataagat gaacactaag cagtgtagaa
    25681 aaacttgtca ttgataatag cggccgagcg ttacgcgctc gttatcacca tagtcacggc
    25741 aggtttcgac gtcatgatac cccgcgagga taaatgcttg tcgcaccgct tcgccctgct
    25801 gccagccatg ttccagaagc agaaagccgc cggataccag cgcgttacgc gactgttcga
    25861 tgatatgcac gatgtctgcc attccactgt ctgccgcaac cagcgcagtg agcggctcaa
    25921 agcggacatc gccttgttga agatgtgggt cctgctcgtc aatatacggc ggattgctga
    25981 caatcatcgc aaactgctgc ccggctagcg cgctaaacca gtcgctttgc agaatgtgga
    26041 tatttttgat cgccagatgc tgggcattac gttgtgccag ggagacagca tcaggcatac
    26101 gatctacagc gataatttcg cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc
    26161 cggtacccgt cccgagatcg agaatacggc aaggttgttc aggcaaccgc gccagtgcct
    26221 gctccaccag acactccgta tccgggcgcg gaattaaggt cgctggcgaa acaaataacg
    26281 gcaacgacca gaattctcgc accccggtta aatgagcaat gggttcacca tcgcgacgac
    26341 gtgtcagtag cgcatcaagt tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg
    26401 cgagaataaa agtacgccct ctgccggtaa catgctccag caggatttca gcatcacgcc
    26461 gcgggctttc gctcgcctga agttggctta ttgcttcacg taaccagtgt tgatattcca
    26521 ttattcctgc tcggacaacg ccgccagttg gtcggcctga tgttcctgga taatcggttc
    26581 aatcagcata tccagcttac cttccatcac ttcatccagg cggtagagcg tcaggttgat
    26641 gcggtgatcg gtaacgcgcc cctgcgggaa attgtaagta cggttacggt cgctgcgatc
    26701 gccactcccc agcaggttac gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc
    26761 agcagcgtgg atgcgagcac cgagcactga aagcgccttc gctttgtttt tatgttgtga
    26821 acgttcgtcc tgacattcaa caacaatccc ggtcggcaag tgagtaatac gaattgccga
    26881 atcggtggtg ttaacgtgct gaccacccgc ccctgacgag cggaaagtat caatgcgtaa
    26941 atctgctggg ttgatgtccg gcagttctgc gtcaggcagt tctggcatta ccgcaacggt
    27001 acaagcagaa gtatgaatac gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg
    27061 accgccggat tcaaatttca gacgaccata cacaccatca ccgctaattt tggcgatgat
    27121 ctctttataa ccaccatgtt caccctcgct ggcgctcatg atttctaccc gccagcggcg
    27181 ggcttcggcg taacggctgt acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc
    27241 gccgccggtt ccggcacgga cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg
    27301 tagtaataga acctgtaatt gctgttccag ttgctcgctt ttttctttag cttcgcgcag
    27361 ttcatcctgc gccatctcac gcatttcagg atcgtcgagc atcatctgtg cggtttcgat
    27421 atcttcctga acctgttgcc agtcggtaaa acagcgcgaa acatcactta actgcgcata
    27481 ttcgcgtgat aatgcgcgaa aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa
    27541 cgcctgaact tcttcatggc gttcatgcag ggcttccagt ttggcaacga tagaaggctt
    27601 cataggcgta aatgcaccct gtaaaaaaag aaaatgatgt actgctactc cagcccgagg
    27661 ctgtcgcgca gaatattcag gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat
    27721 ttcgttggcg catggatcaa gcggttagtc agtttccatg ccagatcctg cataatggct
    27781 tgcgcgtcgc cgccctgctc aagggccgct aacgctttgg cggttaactc atcgcgaacg
    27841 tgctctgcct ggctgcgata ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac
    27901 gccataaatt cgctggtttc ctgagcgaca atagtttccg cctcaaccgc tgcggcttta
    27961 cgctgcgcca ggttgtgcga aatgatgctt tgcagatcgt ccacactata aagataagca
    28021 ttcgccagtt tgccaacttc cggctcaaca tcgcgcggaa cggcaatatc caccaacagc
    28081 attggttggt tacgacgact ttttaatgcg cgctccacca tgcctttccc gataatcggt
    28141 aacgggctgg cggtggaact gatgatgata tcggcttcgc gcagacgttc gtcgatctca
    28201 ctcagggcaa tcacttccgc gccaacttca tctgccagaa tttgggcacg ttcgcgagtg
    28261 cggttggcga taatcatctt ctgtacttta tgttcgcgca gatgacgcgc taccagctcg
    28321 atggtttcgc ccgcgcctac cagcaacact gtgaccgtag agagcgattc aaagatctgc
    28381 cgcgccagcg tacaagccgc aaaagcgaca gacacagcac tggcaccgat atctgtttca
    28441 gtgcgaacgc gtttcgctac agagaaagat ttctggaaca tgcgttccag ttcgctggcc
    28501 ttcatatgac ctttttgcga atcgacaaac gcttttttaa cctgaccgag gatctgcggc
    28561 tccccaagaa ccagtgaatc caggccgctg gcaacacgca ttaaatggct aaccgcgtcg
    28621 ttatcctgat gccagtagag gcttttacgc agatcttctt cattaagatt gtgataatcg
    28681 caaagccagc ggattaacgc ctcttgcagg ttatcctgct cttcaacgct aagataaagt
    28741 tccgtgcggt tgcacgtcga cagcaccacg ccgccctgca ccatcggctg cgcaagcagg
    28801 ctgtcaagcg cctgatcgag cttatccggc gaaaacgata cacgttctcg cagcgataca
    28861 ggtgccgttt tatggttgat accgagtgct aaaagggtca tgtctgcggg aaataatacc
    28921 aacgttgata gggttagtct gcttgcatca tacaggatgc gtaagatcaa taaaagagag
    28981 cgcccccttt tggagtaatt gccgaaagcc gttagattct ggcaattaag acaacttgaa
    29041 catagacgat agcggacggt aacgctagca ttaagggtta taactgcaac gtatctcaag
    29101 gacttgtcat cactatgccc ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg
    29161 ctcttgtgct cactgcctgt tccgttacca cgcccaaagg tcctggcaaa agcccggatt
    29221 cgccacaatg gcgtcagcat cagcaagacg tgcgcaatct taatcagtat cagactcgcg
    29281 gcgcgttcgc ttatatttct gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa
    29341 ccggccagga tcgctaccgt ctgctgctca ctaacccatt gggcagcacg gaactggagc
    29401 tgaatgctca accgggtaac gtgcagttag tcgacaataa aggtcagcgt tataccgccg
    29461 atgacgccga agagatgatt ggcaaattga ccggaatgcc aattccgctc aacagcttgc
    29521 gccagtggat tttaggttta ccgggtgatg caaccgacta caaactggac gaccagtacc
    29581 gcctgagcga aattacctac agccagaatg gcaaaaactg gaaggttgtt tatggtggtt
    29641 atgacaccaa aacgcaacct gcgatgccag ccaatatgga actcaccgac ggtggtcaac
    29701 gcatcaagtt aaaaatggat aactggatag tgaaataatg cggacacagt ggccctctcc
    29761 ggcaaaactt aatctgtttt tatacattac cggtcagcgt gcggatggtt accacacgct
    29821 gcaaacgctg tttcagtttc ttgattacgg cgacaccatc agcattgagc ttcgtgacga
    29881 tggggatatt cgtctgttaa cgcccgttga aggcgtggaa catgaagata acctgatcgt
    29941 tcgcgcagcg cggttgttga tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag
    30001 cggtgcgaat atcagcattg acaagcgttt gccgatgggc ggcggtctcg gcggtggttc
    30061 atccaatgcc gcgacggtcc tggtggcatt aaatcatctc tggcaatgcg ggctaagcat
    30121 ggatgagctg gcggaaatgg ggctgacgct gggcgcagat gttcctgtct ttgttcgggg
    30181 gcatgccgcg tttgccgaag gcgttggtga aatactaacg ccggtggatc cgccagagaa
    30241 gtggtatctg gtggcgcacc ctggtgtaag tattccgact ccggtgattt ttaaagatcc
    30301 tgaactcccg cgcaatacgc caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag
    30361 caatgattgc gaggttatcg caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg
    30421 gctgttagaa tacgccccgt cacgcctgac tgggacaggg gcctgtgtct ttgctgaatt
    30481 tgatacagag tctgaagccc gccaggtgct agagcaagcc ccggaatggc tcaatggctt
    30541 tgtggcgaaa ggcgttaatc tttccccatt gcacagagcc atgctttaag ccgggcaagc
    30601 tgagtttcgg tgacaacgtc accttgttcc agacgttgca tcgcgctctt taatacaccg
    30661 cctggaaagg atcatgcctg gcccgcacag ttttcggcag attctttcca ccaatggacg
    30721 catgcctgag gttcttctcg tgcctgatat gaagcttttt gctggtaacg ccaccccggt
    30781 actagcacaa cgtattgcca accgcctgta cacttcactc ggcgacgccg ctgtaggtcg
    30841 ctttagcgac ggcgaagtca gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt
    30901 catcatccag tccacttgtg cccctactaa cgacaacctg atggaattag tcgttatggt
    30961 tgatgccctg cgtcgtgctt ccgcaggtcg tatcaccgct gttatcccct actttggcta
    31021 tgcgcgccag gaccgtcgcg tccgttccgc tcgtgtacca atcactgcga aagtggttgc
    31081 agacttcctc tccagcgtcg gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca
    31141 gattcagggt ttcttcgacg ttccggttga taacgtattt ggtagcccga tcctgctgga
    31201 agacatgctg cagctgaatc tggataaccc aattgtggtt tctccggaca tcggcggcgt
    31261 tgtgcgtgcc cgcgctatcg ctaagctgct gaacgatacc gatatggcaa tcatcgacaa
    31321 acgtcgtccg cgtgcgaacg tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg
    31381 tgactgcgta ctggtcgatg atatgatcga cactggcggt acgctgtgta aagctgctga
    31441 agcgctgaaa gaacgtggtg ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc
    31501 tggcaacgcg gcgaacaacc tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac
    31561 cattccgctg agcgatgaaa tcaaatcact gccgaacgtg cgtactctga ccctgtcagg
    31621 tatgctggcc gaagcgattc gtcgtatcag caacgaagaa tcgatctctg ccatgttcga
    31681 acactaatcg aacccggctc aaagacccgc tgcggcgggt ttttttgtct gtaatatcca
    31741 tttgtatgac ctatgcctcc ttcacctgcc atttagttga cagatgatgc gctcacggat
    31801 gaaacattat tgtgaacaaa atattttcct cacatgtgat gcctttccgc gctctgatcg
    31861 acgtttgctg gaaagaaaaa tatactgccg cacggtttac ccgtgacctg attgccggga
    31921 taaccgtcgg gattattgct atcccgctgg cgatggcgtt ggctattggt agtggtgtgg
    31981 ccccccagta cggtttatat accgcagctg ttgcggggat tgtcattgct ctaacgggtg
    32041 ggtcacgctt tagcgtttcc ggtccgactg cggcatttgt ggtaattctc tatcccgttt
    32101 cgcaacagtt tggactggca ggactgctgg ttgcgacctt gttgtcggga atctttttga
    32161 ttctgatggg gctggcacgc tttggtcgtc tgattgagta tattccggtt tccgtcacct
    32221 taggtttcac ctcgggtatc gggatcacca tcggtaccat gcagattaaa gattttctcg
    32281 gtctgcaaat ggcccatgtc ccggaacatt atctacaaaa agtcggcgca ttatttatgg
    32341 cgctgccgac cattaatgtg ggtgatgctg ccattggcat tgtgacgctt ggcattctgg
    32401 ttttctggcc gcgtctgggc attcgtttac ccggtcacct tccggccttg ctggctggtt
    32461 gcgcggtgat ggggattgtt aacctgctcg gcggacatgt tgctaccatc ggttcgcaat
    32521 tccactacgt cctggccgat ggttctcagg gtaacggtat tccgcaactg ctgccgcaac
    32581 tggtgctgcc gtgggatctg cctaattcag aattcacgct aacctgggat tctattcgca
    32641 ccctgctgcc tgcggcattc tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg
    32701 ctgtggtact ggatggtatg accgggacta agcacaaggc gaatagtgaa ctggtcggtc
    32761 aggggctggg gaatatcatc gctccattct ttggtggtat taccgctact gctgccatcg
    32821 cgcgttctgc cgctaacgtc cgtgccgggg caacttcccc tatctcggcg gtgatccact
    32881 ctattctggt tattctcgcc ctgctggtac tggcaccact gctctcctgg ctgccgcttt
    32941 ccgccatggc agccctgctg ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg
    33001 tcgacttgct gcgtcatgcg ccgaaagatg acatcatcgt catgctgctg tgtatgtcac
    33061 tgaccgtgct gtttgatatg gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc
    33121 tgtttatgcg tcgtatcgca cgtatgactc gcctggcacc ggtagtcgta gatgttccag
    33181 acgatgtcct ggttctgcgc gttattggcc cgctgttttt tgctgctgct gaaggcttat
    33241 tcacggacct ggagtcacgt cttgaaggca aacggattgt gattctgaag tgggatgccg
    33301 ttccggtact tgatgctggt ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg
    33361 aaggatgtga actgcgcgtg tgcaacgtgg aattccagcc actgcgaact atggctcgcg
    33421 caggcattca accgatcccg ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg
    33481 cggatttata agtacgagat tgaccagtca gcgcaaactg actggtcagc aaactgcatt
    33541 atttgttagc tatgacggcg attatcgcta cggcggcaac gtttgtcata gtgacgaacc
    33601 caccagtaac ggtcgcacac ctgttcatgc ccgccaacac gggcacccgc cagccagaga
    33661 atggcaccga cgaaaatact tagcactgca ccatgtgcga aatactgggg catattaaac
    33721 tgtggtaact ggttgaggat tgaatacccc acgccgacca ccattaccac cagacccaac
    33781 cccatgagca cgttaccgag tatcgaagcg tttttgcgtt tcatatgtca cctccggaac
    33841 tttctgggtt gtaacaggga atacccctct tccttatgtg taaagtatag acaacaccct
    33901 gtgggttatg tgcgggcgta atcacaatta caacccttat ttcaacaaaa ctttacaaat
    33961 aaacgcctga acaccttgac tttctaatag tccactcagg caattattac gttatctgca
    34021 tttgatacgc agttttttgt caagcggggc cgcaaccagt aaactacgcg ccagttatgt
    34081 acacactcag gacaaaaaaa cgtgacgatt aaattgattg tcggcctggc gaaccccggt
    34141 gctgaatacg ccgcaacgcg acataatgct ggtgcctggt tcgttgactt actggcagag
    34201 cgtttgcgcg ctccgctgcg cgaagaggct aaattctttg gttatacttc gcgagtcacc
    34261 cttggaagcg aagatgtccg cctgttagtc ccgactacat ttatgaatct cagcggcaaa
    34321 gccgttgcgg cgatggccag ttttttccgc attaatccgg acgaaattct ggtggcccac
    34381 gacgaactgg atctgcctcc tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt
    34441 cacaatggac tgaaagacat catcagtaaa ttgggtaata accctaactt tcaccgttta
    34501 cgcatcggaa tcggtcatcc gggcgataaa aataaagttg tcggttttgt gttaggcaaa
    34561 ccgcctgtta gtgaacagaa gttaattgat gaagccatcg acgaagcggc gcgttgtact
    34621 gaaatgtggt ttacagatgg cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg
    34681 caataagtcg ttgtctgcgg catttttgcc gagtgccgtg tataataggc aaagttattt
    34741 ccatttctgc aatctgttag caataacagg ttgattatta agatattaag gtgatttaaa
    34801 tcatgggatt caaatgcggt atcgtcggtt tgcccaacgt cgggaaatct accctgttca
    34861 acgcgctgac caaagccggt attgaagcgg ccaactttcc attctgcacc attgagccga
    34921 acacaggcgt cgtaccaatg cctgaccctc gcctggatca actggctgaa atcgtaaaac
    34981 cgcaacgtac gcttcccacg accatggaat ttgtcgatat cgccggtctg gtaaaaggcg
    35041 catcgaaagg cgaaggtctg ggtaaccagt tcctgaccaa catccgtgaa accgaagcga
    35101 tcggtcacgt tgttcgctgc tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta
    35161 acccggctga cgatattgaa gttatcaaca ccgaactggc gctggcagac ctcgacacct
    35221 gcgaacgtgc gattcatcgc gtacagaaga aagccaaagg tggcgataaa gacgcgaaag
    35281 ctgagctggc ggtcctggaa aaatgcctgc cccagctgga aaacgcaggt atgctgcgcg
    35341 cgctggattt aagcgctgaa gagaaagcgg ctattcgtta cctgagcttc ctgacgctga
    35401 aaccaacaat gtacatagcc aacgtcaacg aagatggttt tgaaaacaac ccatatcttg
    35461 accaggtgcg tgaaatcgcg gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg
    35521 ttgaagcaga cattgccgaa ctggacgacg aagaacgtga cgagtttatg caggagcttg
    35581 ggctggaaga accgggcctg aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc
    35641 aaacttactt caccgctggg gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa
    35701 ccgcgccgca ggcagcgggc aaaatccata ctgattttga aaaaggcttt atccgtgcac
    35761 aaaccatctc gtttgaagat ttcatcactt acaaaggtga acaaggcgcg aaagaagcag
    35821 gcaaaatgcg tgcagaaggt aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc
    35881 ttttcaacgt ctaattaatt tattgtctca tgaggtttcg ctaaatctca tgaagagcgc
    35941 aaaagtatat aaaatccacg caattgcgtg gatttttcat ttcaccgagt cctaatcatt
    36001 cttgtaacat cctacacttt tgtgcttatt gcctacagtg aaccacgaga ataattaaag
    36061 acataagtgt tcagatgact gatatctcat tagcagtgat ataacgtcag tttttgttcc
    36121 actacacatc agccctcttc acagctgatc aatattgatc tcgctccaat atgctggcga
    36181 gaaaaggact tacctgaagg gtaatatccc gcagataaag tccccgaata acgtgattac
    36241 gtgcgatcct ggcaagatga aaatgataac cagtagcaca ataataacga taggccagaa
    36301 aatccattga acaaaaatga atatcgatgt tttttgcttt ttcttattag taatgcaaaa
    36361 atggcgcata ttgtttttgt tatgcagata catcaagcga aaatcccata actaattagg
    36421 attattacaa aaaatcttca ccaaattttt taaccgctgt ttgttgtaat atttttgcca
    36481 tattcatagt aacgaattgg aataagtaac gacaacttag ggaataactc gatataaact
    36541 cacctttgta aggggggggt taaatatata ttaattcctg tgttcctgat tttgcccggt
    36601 aatagagaat tattctcaga gaagctcatc atgattgcac catacagata tcatgatgat
    36661 aagaggatat tattcattga cttaaacaaa atgacaaaat ccatagaccc atagcctggg
    36721 tactatatag gagtggtatg ggcatcaaac aacacaatgg gaataccaaa gccgatcgtc
    36781 tcgctgaatt aaaaatccgt tcgccctcaa ttcaactgat aaaatttggc gctattggtt
    36841 tgaatgcaat tatcttttcc cccctgctga tagctgctga tacaggaagt caatatggca
    36901 ccaatattac tattaatgat ggtgacagaa ttacaggaga taccgccgat ccatcaggaa
    36961 acctctatgg tgtaatgacc ccagcaggaa acacgcctgg caatatcaac ctgggtaatg
    37021 atgtcaccgt caatgtcaac gacgcctctg gatatgcaaa aggaatcatt attcagggca
    37081 aaaacagctc cctgacagct aaccgactca cagtagatgt tgttggtcaa acctctgcca
    37141 tcggcattaa cttaattggt gactataccc atgctgactt aggcacaggc agcaccatta
    37201 agagtaacga tgacggcatc attattgggc atagctcaac actaacagcc actcaattca
    37261 ccattgaaaa ctcgaacggt ataggcctaa ccatcaatga ctatggcacc agtgtcgatc
    37321 ttggaagcgg aagtaaaatc aagaccgatg gaagtacagg tgtttatatc ggtggtctca
    37381 acggcaataa cgccaatggt gctgcgcgtt ttacggcgac agacctgaca atcgatgttc
    37441 agggctacag cgccatgggg ataaacgtac agaaaaactc tgttgtcgat ctcggaacaa
    37501 acagtaccat taaaaccaat ggcgataatg ctcacggcct ctggagcttt ggccaggtta
    37561 gcgcgaatgc actcactgtt gatgtaactg gagccgcggc caatggcgtc gaagttcgtg
    37621 gtggtacaac cactatcggt gcagatagcc atatttcttc cgcgcagggc ggtggcctcg
    37681 tcaccagtag ttcagacgcg acaatcaatt tttctggcac ggcagcgcaa cgaaacagca
    37741 tcttttccgg cggttcttat ggtgcctcgg cccagacggc aacggctgtt atcaacatgc
    37801 aaaataccga tattacggtt gatcgtaatg gcagtctggc gctgggtttg tgggcgctca
    37861 gcggcggtag aataaccgga gacagtttgg ctatcaccgg cgcggcagga gccagaggga
    37921 tttatgccat gaccaacagc cagatcgacc tcacgagcga tctggtcatt gatatgagta
    37981 cacccgacca gatggccatc gcaacgcaac atgacgatgg ttatgccgcc agccgcatca
    38041 acgcctcggg tcgtatgctt atcaacggta gcgttctttc caaaggtggg ctaatcaatc
    38101 tggatatgca ccctgggtcg gtttggacag gttcctccct cagcgataat gtcaatggcg
    38161 ggaaactgga cgttgcaatg aataacagcg tctggaacgt aacaagtaat tctaatctcg
    38221 acacgctggc gctgagccat tcaactgtcg attttgccag ccacgggtca actgccggca
    38281 catttaccac attaaacgta gagaacctga gcggtaacag tacctttatt atgcgtgctg
    38341 atgttgttgg cgagggtaat ggcgttaata atagagggga tttattgaat atcagcggga
    38401 gtagtgctgg taatcacgta ttggctatcc gcaaccaggg cagcgaggcc acaacgggaa
    38461 atgaagttct gacagtggta aaaaccactg acggcgcggc ctcgttcagc gcgtcttctc
    38521 aggttgagtt ggggggatat ctgtacgatg tgcgtaaaaa tggcactaac tgggagcttt
    38581 acgcttccgg gacagttccg gaaccgactc ctaatcctga acccacacca gctcccgctc
    38641 agcctcccat agtcaacccc gatcctacgc ctgaacccgc tcccacgcct aaacccacca
    38701 cgaccgcaga tgctggcggc aattatctca atgtcggtta cttattgaac tatgttgaaa
    38761 accgtacgct gatgcaacgg atgggtgact tgcgaaatca gagtaaagac ggtaatatct
    38821 ggttgcgcag ttatggggga agcctggact cctttgccag tggcaaactg agcggctttg
    38881 acatgggtta cagcggtatc cagtttggtg gggataaacg tctctctgat gtaatgccgt
    38941 tgtatgtcgg tctgtatatt gactcaacac atgcatcgcc ggactatagc ggaggcgacg
    39001 gtaccgcacg ttcagactac atgggaatgt acgccagtta catggcacaa aacggttttt
    39061 acagcgatct cgttataaaa gcatcgcgcc agaaaaatag tttccacgta ctggacagtc
    39121 agaacaacgg cgttaacgcc aacggcactg cgaatggaat gagcatctcc ctggaagccg
    39181 ggcagaggtt caacctgtcc cctactggtt atgggttcta tatagagccg caaacccagc
    39241 ttacatacag ccaccagaat gagatggcta tgaaggcgag taatggcctc aatatacatc
    39301 tgaatcacta cgaatcgctg ctggggcgtg ccagcatgat actggggtat gacatcaccg
    39361 caggcaacag ccagctgaat gtctatgtga agactggcgc tatccgcgag ttttcagggg
    39421 ataccgaata tctgttgaac gactcccggg agaagtacag tttcaaaggt aatggctgga
    39481 ataacggcgt gggagtcagt gcacagtata acaaacagca cacattctat ctcgaagcgg
    39541 attacacgca gggtaacctc tttgatcaga agcaagtcaa cggaggatat cgcttcagct
    39601 tttaagtgcg ggatagacaa gcccttaaac gatgctgaaa attgttagcg tgcgccggaa
    39661 tgaaatacag atcactctga tgtatcagaa atcgtttctg tctttcatac ccggcgctta
    39721 aactgccctg catcaatgcc atgttgcttc attttccgcc acagcgtagt gcggccgatc
    39781 ccaagtaaag ccgacatttc ctgaatgcga ccgcctgtga cctgggctgc gttaataatt
    39841 gcctcttttt caacttccgc aaatgacaga ctggtggaaa ggcgggtggc gctgacatca
    39901 tctgtcgcct gctcggtaaa cagatgttca ggtaaatcac tgacgcgaat gcgcccgtta
    39961 tcactactca gagcaagatt ctcgatgacg ctgtaaagtt caaaatcgtt gcctggccat
    40021 gcacaagaaa ctaggcgagc gagggcatcg tcatcaattt tcagccgcgt agagaagcgt
    40081 ttttcaagac tgcgtaattt gttattcacc agcgccggaa tgctgccacg ccgcatacgc
    40141 agaggcggga tggtaatttc aaatgcatgc agcgcgtaat acagctggcg actaaaacga
    40201 ttttgttcca ccagcattgc gaggtccgcg gtcgttgttg caatcacttt gacatcaatt
    40261 ggtattaaac gccgcgcatc cagtcgcgtg ataaccccct gcttgataac ctgcagcaaa
    40321 gcagactgta actccaccgc cagatattca atcttttcaa gaaacagcgt gccgccgtgt
    40381 gccagttcca gccgactcag acggccattt tcattgtccg tgcgatcgcc accaataaat
    40441 tcttccgcca gcgcagcatc accatataac tcacaattga cggcgatata aggacctgca
    40501 gcacgctcgc tttcattatg aattgcctga cttagcagtg ccttgcccac gccctcttct
    40561 ccacaaagca ggacaggaaa gctactgcgc gccgcctggc gaccaaaatg aatcaagcgg
    40621 cgggtttgcg gatcgtcctg tggcatatga gcgaaggtat ggctgacttt tcctaattga
    40681 ctggtcatta actgccgcat ctgttccaca ggatggagca acaaaataaa gctggttccc
    40741 tgcgtttcta ttatcggttt aagggttatc accgcatcaa taaactggtg ctggctttca
    40801 aaggttgctt ctacgtgttt gagcggatgt gccagtttta ttgcttgttg caatacggcg
    40861 ggtaacgtta agagttcagt gattgcccgt ccctgacttg ccgtcgcgtc aaggcgcaag
    40921 acccgcgccg cctgggcatt aataaattgc aaattaccct gctcgtccca gctaatcacg
    40981 ccatcatcca tactttctaa cagggcatta agttgattta aatgacggtt agtttcagcg
    41041 agcaaactgt ccgtcagcag taaattaccg acctcgcggg cgattgccaa cgtcaacggc
    41101 aaatcagctg cggtagtttg ctcaaccgga cacgccagcg ctattgttcc cgtcaatcgg
    41161 cccttgctgt caaacaacgg cgttgcacaa aaggcccagt tccagagtgc ctgtttgaaa
    41221 tgttgatcgg ccatcgtttt cacggcctga ccagagatag ccgctaacga tagcgcacaa
    41281 gtaccaataa ttccctcggc gcaatacgtg ccgtcattga atcccagtgc acttagctgc
    41341 tgcaaggttt gcggatcgcc attacggctg agaatgcagg cggtttcatc gaggataaac
    41401 agcgcacact ctcgcggtgc catatattcc caggcatctt ccagcgcagc ctgcccgagc
    41461 gtcagcatcg ctttcttacg ccgataaata gaagcaaatg tcacgccctg ggcctgatgt
    41521 ggtacgttcc atgtctcccg tttcatcagc ttattgcatc gctcccagga ggttgcaatt
    41581 aagggagata tgccccgacc atcgttgtta aaagcgccac tcatatcccg catcccttat
    41641 gacaaaaggt gttccggctc gcaattttaa gtattaatac acgtaaaagt ttcaatatgg
    41701 aacattcatt aaagcatatg tttcactatg aaacataatt tgggctaatt ttcttttccc
    41761 ttgcctgatg cacaatggat tcatcggcag tcagtggtcg ccgtgtcgtt gaacatcatc
    41821 catgccctac cgtaattgct ggagcaaaat aatgaaaaaa ttgatcaatg atgtgcaaga
    41881 cgtactggac gaacaactgg caggactggc gaaagcgcat ccatcgctga cactgcatca
    41941 ggatccggtg tatgtcaccc gagctgatgc ccctgttgca ggaaaagtcg ccctgctgtc
    42001 gggtggcggc agcggacacg agccgatgca ctgtggttat atcggtcagg ggatgctttc
    42061 gggggcctgt ccgggcgaaa ttttcacctc accgacgccc gataaaatct ttgaatgctc
    42121 catgcaaatt gatggcggcg aaggtgtact gttgattatc aaaaattaca ccggcgatat
    42181 tcttaacttt gaaacagcga ccgagttact gcacgatagc ggcgtaaaag tgaccactgt
    42241 ggtcattgat gacgacgttg cggtaaaaga cagtctttat accgccgggc gacgcggcgt
    42301 tgccaacacc gtattaattg aaaaactcgt aggcgcagcg gcggagcgtg gcgactcact
    42361 ggacgcctgt gcggaactgg ggcgtaagtt gaataatcaa ggccactcaa taggtatcgc
    42421 tctcggtgcc tgtaccgttc ctgccgcggg caaaccttct tttacccttg cggataatga
    42481 gatggagttt ggcgtaggca ttcatggtga gccaggtatt gaccgccgcc ctttctcttc
    42541 ccttgatcaa accgtcgatg aaatgttcga caccctgctg gaacatggct cataccatcg
    42601 cactttgcgt ttctgggatt atcaacaagg cagctggcag gaagaaccac aaaccaaaca
    42661 accgctccag tctggcgatc gggtgattgc actggttaac aatcttggcg caactccgct
    42721 ttctgagctg tacggcgtct ataaccgcct gaccacacgt tgccagcaag cgggattgac
    42781 tatcgaacgt aatttaattg gcgcgtactg cacctcactg gatatgaccg gtttctcaat
    42841 caccttactg aaagttgatg acgaaacgct ggctctctgg gacgccccgg tccacacccc
    42901 ggcccttaac tggggtaaat aaggagaaag caatgtcact gagcagaact caaattgtta
    42961 actggctcac tcgttgtggc gatattttca acaccgagag cgagtatctt accgggctgg
    43021 atcgcgagat tggcgacgct gaccacgggc taaatatgaa ccgaggcttt agcaaagtgg
    43081 tggaaaaact ccctgctatc gcagataaag atatcggttt cattctcaaa aataccggta
    43141 tgacgctgct ttccagcgtc ggtggtgcca gtggtccgct gttcggtacc ttctttatcc
    43201 gcgccgcaca ggcgacccag gcacggcaaa gcctgacact ggaagagctt tatcagatgt
    43261 tccgcgatgg cgcggacggc gtaatcagtc gcgggaaagc cgaacctggc gataaaacca
    43321 tgtgtgatgt gtgggtgccg gtggtggaat cgttacgtca gtccagcgag caaaatctct
    43381 ctgttccggt ggcgctcgaa gctgccagta gcatcgccga atccgctgca caaagtacga
    43441 ttacgatgca agcccgcaaa ggccgcgcca gttatctcgg tgaacgcagt attggtcacc
    43501 aggatcccgg cgcgacctcg gtgatgttta tgatgcaaat gttggcgtta gccgcaaaag
    43561 agtaaggaat tggtgatggt aaacctggtc atagtttcac atagcagccg actgggagaa
    43621 ggtgtcggtg aattagcccg tcagatgtta atgagtgata gttgtaaaat cgccattgcc
    43681 gcgggaattg acgatccaca aaatcccatt ggtaccgatg ccgtcaaagt gatggaggcc
    43741 atcgaatctg ttgctgatgc cgaccatgtg ctggtcatga tggatatggg tagcgcatta
    43801 ttgagtgctg aaactgcgct ggaattgctg gctcccgaga tcgccgcaaa agtacgtttg
    43861 tgtgctgcgc cgttggtcga aggtacactg gcagcaacgg tcagcgcggc ctcgggggcg
    43921 gatatcgaca aagttatctt tgacgctatg catgcgctgg aagccaaacg tgaacaactg
    43981 ggtttaccgt cctccgacac tgaaatctct gacacatgtc ctccgtacga tgaagaagcc
    44041 cgttctctgg cggtggtcat aaaaaaccgt aacggcctgc atgtacgtcc ggcctcccgg
    44101 ctggtttata ccttatcgac atttaatgcc gatatgttgc tggaaaaaaa cggcaaatgc
    44161 gtcacaccag agagtattaa ccagattgcg ttactacaag ttcgctataa cgatacgctg
    44221 cgcctgattg cgaaagggcc agaagctgaa gaggcactga tcgctttccg tcacctggct
    44281 gaagataact ttggtgaaac ggaggaagtc gctccaccta ctctgcgtcc cgttctgcct
    44341 gtttcgggta aagcctttta ttatcaacca gttttatgta cggtacaggc aaaatcaacc
    44401 ctgaccgcgg aagaagaaca agatcgatta cgccaggcta ttgacttcac gttattagat
    44461 ctgatgacgt taacagcgaa agcagaagcc agcgggcttg acgatattgc cgcaatcttt
    44521 tctggtcacc atacactgtt aggtgatccg gaactgctgg cggcggcaag cgaactcctt
    44581 cagcatgaac attgcacggc agaatatgcc tggcagcaag ttcttaaaga acttagccag
    44641 caataccagc aactggatga tgaatatcta caagctcgct atattgatgt ggacgatctt
    44701 ctgcatcgca ccctggtcca cctgacccaa acgaaagaag aactcccgca gtttaactcg
    44761 ccaactattc tactggcgga gaacatttat ccttccacag tactgcaact ggatccggcg
    44821 gttgtaaaag gtatctgcct tagcgccgga agtccggtat cccacagcgc cctaatcgcc
    44881 cgtgaactgg ggattggctg gatttgccag cagggtgaga aactgtatgc gatacaacca
    44941 gaagaaacgc taacgctgga cgttaaaacg caacgtttca accgtcaggg ttaagccacc
    45001 gttcggatgg catcgttctg atgtcatccg gcaatttacc cagcactgat gttttttaac
    45061 tacctgttat attttttacc aatttatgat gatttttctt cgctaatcgc gaaccaaatg
    45121 tcgctggctg gctaataaac agacgttatt tgatcactct atttcgccgt tatttatccc
    45181 tttaattccc ttttctaaaa tgcctgaccg ttcgcagaat gagatttcga tcatgcagct
    45241 agtgcgatcc tgaactaagg ttttctgata cttgaatacc gttttattcg gtttcgccaa
    45301 aggagaatga ttgatgaaat cccccgcacc ttctcgcccg caaaaaatgg cgttaattcc
    45361 agcctgtatc tttttgtgtt tcgctgcgct atcggtgcag gcagaagaaa caccggtaac
    45421 accacagccg cctgatattt tattagggcc gctgtttaat gatgtgcaaa acgtcaaact
    45481 ttttccggac caaaaaacct ttgccgatgc cgtgcctaac agcgatccgc tgatgatcct
    45541 tgctgattat cggatgcagc aaaaccagag cggatttgat ctgcgccatt tcgttaacgt
    45601 caatttcacc ctgccgaaag aaggcgagaa atatgttccg ccagaggggc agtcactgcg
    45661 cgaacatatt gacggacttt ggccggtatt aacgcgttct accgaaaaca ccgaaaaatg
    45721 ggattctctg ttaccgctgc cggaacctta tgtcgtgccg ggcggacgtt ttcgcgaggt
    45781 atattactgg gacaattact tcaccatgtt aggacttgcc gaaagtggtc actgggataa
    45841 aatcgcggat atggtggcca attttgctca tgaaataaac acttacggtc atattcccaa
    45901 cggcaaccgc agttactatt taagccgctc gcaaccgccc ttctttgccc tgatggtaga
    45961 gttactggcg cagcatgaag gcgatgccgc gttgaagcaa tacctgccgc aaatgcaaaa
    46021 agaatatgct tactggatgg acggtgttga aaacctgcaa gccggacaac aggaaaaacg
    46081 cgttgtaaaa cttcaggata gtacccttct caaccgctac tgggacgatc gcgatacgcc
    46141 acgaccagag tcatgggtgg aagatattgc caccgccaaa agcaatccga atcgacctgc
    46201 cactgaaatt taccgcgacc tgcgctctgc cgctgcgtct ggctgggatt tcagctcgcg
    46261 ctggatggac aacccgcagc agttaaatac cttacgcacc accagcatcg taccggtcga
    46321 tctgaacagc ctgatgttta aaatggaaaa aatcctcgcc cgcgccagca aagctgcagg
    46381 agataacgcg atggcaaacc agtacgaaac gctggcgaat gcccgtcaaa aagggatcga
    46441 aaaatacctg tggaacgatc aacaaggctg gtatgccgat tacgacctga aaagtcataa
    46501 agtgcgcaat cagttaaccg cggccgccct gttcccgctg tacgtcaatg cggcagcgaa
    46561 agatcgcgcc aacaaaatgg cgacggcgac gaaaacacat ctgctgcaac ccggcggcct
    46621 gaacaccacg tcggtgaaaa gtgggcaaca atgggatgcg ccaaatggct gggcaccgtt
    46681 acagtgggtc gcgacagaag gattacaaaa ctacgggcaa aaagaggtgg cgatggacat
    46741 tagctggcac ttcctgacca atgttcagca cacctatgac cgggagaaaa agctggtgga
    46801 aaaatatgat gtcagcacca ccggaacggg gggcggcggt ggcgaatatc cattacagga
    46861 tggctttggc tggaccaatg gcgtgacgct gaaaatgctg gatttgatct gcccgaaaga
    46921 gcaaccgtgt gacaatgttc cggcgacgca tccgaccgtt aagtcagcaa cgacgcaacc
    46981 ctcaaccaaa gaggcacaac ccacacctta accagcgctt actccgacaa cgtcattcgg
    47041 cttctccgga tggcgttgtc ggcagataac accatatcta tggttttttc tacacagacg
    47101 gtttttcaag tcctggcggg aagtattgcg caaatgtttg ccatacgtgg ttgccgaaga
    47161 tagcgtcatt tctacgggta agtgtttcac tttcatcacc cggcgattta cgccgacgtt
    47221 ggctatataa agcatcaatt gctaccgaat attgttacgg tgttcactca tcaacgtttt
    47281 tttgctttct ggagttaaat taataatcac ataatccaga cactccagag aatttagcaa
    47341 ccaaaaaaag aattcagacc attcctcttc gcgtaactga agcattgccg aacgggtatt
    47401 ttggcttaat ctgccagaag gattgatcaa ttctaaatgt tcaataagcg cagccaaaac
    47461 cgcctcacta tccgccccat ttttaacgcc tgactggcta ctgtctaaaa tatttatttt
    47521 ctgaatcaca ccaaaaacaa cgaaacaata attaaaaaag gattctgttg ttgttaccgg
    47581 gaaccgactt atttcgctca gaaagtcgtt caaccagctc cgcagtgata agattcgctg
    47641 atgatacagt gtatcctcac tgcgtgctgg aggtgccagt ataatatgtt gatcaataca
    47701 gtatttattg tgttcagtcg tttcctgatg cgtttggttc tctgcggatt gtcgctggcg
    47761 aaataaatgg cttgcggtaa atgccgttac agagcagcgc aacgcgtcaa ttccttcgga
    47821 gtgcccgact tttggcgaga gttccggttt tatgcgtcca ggtagcggaa atttcacccg
    47881 aatgagaaat gcattaataa aat
//
LOCUS       DAJVQR010000023        47477 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVQR010000023 DAJVQR010000000
VERSION     DAJVQR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            162..644
                     /gene="sseA"
                     /locus_tag="OQM52_001361"
                     /pseudo
     CDS             162..644
                     /gene="sseA"
                     /locus_tag="OQM52_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1463..2239)
                     /gene="sseB"
                     /locus_tag="OQM52_001362"
     CDS             complement(1463..2239)
                     /gene="sseB"
                     /locus_tag="OQM52_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1563276.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2381..3664)
                     /gene="pepB"
                     /locus_tag="OQM52_001363"
     CDS             complement(2381..3664)
                     /gene="pepB"
                     /locus_tag="OQM52_001363"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1563277.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3723..3923)
                     /gene="iscX"
                     /locus_tag="OQM52_001364"
     CDS             complement(3723..3923)
                     /gene="iscX"
                     /locus_tag="OQM52_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1563278.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3935..4270)
                     /gene="fdx"
                     /locus_tag="OQM52_001365"
     CDS             complement(3935..4270)
                     /gene="fdx"
                     /locus_tag="OQM52_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1563279.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4272..6122)
                     /gene="hscA"
                     /locus_tag="OQM52_001366"
     CDS             complement(4272..6122)
                     /gene="hscA"
                     /locus_tag="OQM52_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1563280.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6139..6654)
                     /gene="hscB"
                     /locus_tag="OQM52_001367"
     CDS             complement(6139..6654)
                     /gene="hscB"
                     /locus_tag="OQM52_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1563281.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6750..7073)
                     /gene="iscA"
                     /locus_tag="OQM52_001368"
     CDS             complement(6750..7073)
                     /gene="iscA"
                     /locus_tag="OQM52_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1563282.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7090..7476)
                     /gene="iscU"
                     /locus_tag="OQM52_001369"
     CDS             complement(7090..7476)
                     /gene="iscU"
                     /locus_tag="OQM52_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1563283.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7504..8718)
                     /gene="iscS"
                     /locus_tag="OQM52_001370"
     CDS             complement(7504..8718)
                     /gene="iscS"
                     /locus_tag="OQM52_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1563284.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8830..9318)
                     /gene="iscR"
                     /locus_tag="OQM52_001371"
     CDS             complement(8830..9318)
                     /gene="iscR"
                     /locus_tag="OQM52_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1563285.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9588..10328)
                     /gene="trmJ"
                     /locus_tag="OQM52_001372"
     CDS             complement(9588..10328)
                     /gene="trmJ"
                     /locus_tag="OQM52_001372"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1563286.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10447..11250
                     /gene="suhB"
                     /locus_tag="OQM52_001373"
     CDS             10447..11250
                     /gene="suhB"
                     /locus_tag="OQM52_001373"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1563287.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11395..12249
                     /locus_tag="OQM52_001374"
     CDS             11395..12249
                     /locus_tag="OQM52_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1563288.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12440..13720
                     /gene="csiE"
                     /locus_tag="OQM52_001375"
     CDS             12440..13720
                     /gene="csiE"
                     /locus_tag="OQM52_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1563289.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13712..14851)
                     /locus_tag="OQM52_001376"
     CDS             complement(13712..14851)
                     /locus_tag="OQM52_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1563290.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15011..15901)
                     /gene="hcaR"
                     /locus_tag="OQM52_001377"
     CDS             complement(15011..15901)
                     /gene="hcaR"
                     /locus_tag="OQM52_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1563291.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16037..17398
                     /locus_tag="OQM52_001378"
     CDS             16037..17398
                     /locus_tag="OQM52_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1563292.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17395..17913
                     /locus_tag="OQM52_001379"
     CDS             17395..17913
                     /locus_tag="OQM52_001379"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1563293.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17913..18233
                     /locus_tag="OQM52_001380"
     CDS             17913..18233
                     /locus_tag="OQM52_001380"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1563294.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18230..19042
                     /gene="hcaB"
                     /locus_tag="OQM52_001381"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18230..19042
                     /gene="hcaB"
                     /locus_tag="OQM52_001381"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1563295.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19052..20269
                     /locus_tag="OQM52_001382"
     CDS             19052..20269
                     /locus_tag="OQM52_001382"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS1563296.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20366..20788
                     /locus_tag="OQM52_001383"
     CDS             20366..20788
                     /locus_tag="OQM52_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1563297.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20836..21708)
                     /locus_tag="OQM52_001384"
     CDS             complement(20836..21708)
                     /locus_tag="OQM52_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1563298.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21720..22814)
                     /locus_tag="OQM52_001385"
     CDS             complement(21720..22814)
                     /locus_tag="OQM52_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1563299.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22847..23845)
                     /locus_tag="OQM52_001386"
     CDS             complement(22847..23845)
                     /locus_tag="OQM52_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1563300.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23870..25381)
                     /locus_tag="OQM52_001387"
     CDS             complement(23870..25381)
                     /locus_tag="OQM52_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1563301.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25404..26386)
                     /locus_tag="OQM52_001388"
                     /pseudo
     CDS             complement(25404..26386)
                     /locus_tag="OQM52_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26483..29584)
                     /locus_tag="OQM52_001389"
     CDS             complement(26483..29584)
                     /locus_tag="OQM52_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCS1563302.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29591..29764)
                     /locus_tag="OQM52_001390"
     CDS             complement(29591..29764)
                     /locus_tag="OQM52_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563303.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29882..31075
                     /locus_tag="OQM52_001391"
     CDS             29882..31075
                     /locus_tag="OQM52_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1563304.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31273..32526)
                     /gene="glyA"
                     /locus_tag="OQM52_001392"
     CDS             complement(31273..32526)
                     /gene="glyA"
                     /locus_tag="OQM52_001392"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1563305.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32853..34043
                     /gene="hmpA"
                     /locus_tag="OQM52_001393"
     CDS             32853..34043
                     /gene="hmpA"
                     /locus_tag="OQM52_001393"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1563306.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34088..34426)
                     /gene="glnB"
                     /locus_tag="OQM52_001394"
     CDS             complement(34088..34426)
                     /gene="glnB"
                     /locus_tag="OQM52_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1563307.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34487..35821)
                     /gene="glrR"
                     /locus_tag="OQM52_001395"
     CDS             complement(34487..35821)
                     /gene="glrR"
                     /locus_tag="OQM52_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1563308.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35811..36524)
                     /gene="qseG"
                     /locus_tag="OQM52_001396"
     CDS             complement(35811..36524)
                     /gene="qseG"
                     /locus_tag="OQM52_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1563309.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36689..38116)
                     /gene="qseE"
                     /locus_tag="OQM52_001397"
     CDS             complement(36689..38116)
                     /gene="qseE"
                     /locus_tag="OQM52_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1563310.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38674..42561)
                     /gene="purL"
                     /locus_tag="OQM52_001398"
                     /gene_synonym="purI"
     CDS             complement(38674..42561)
                     /gene="purL"
                     /locus_tag="OQM52_001398"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1563311.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42820..44376
                     /gene="mltF"
                     /locus_tag="OQM52_001399"
     CDS             42820..44376
                     /gene="mltF"
                     /locus_tag="OQM52_001399"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1563312.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44373..44909)
                     /gene="tadA"
                     /locus_tag="OQM52_001400"
     CDS             complement(44373..44909)
                     /gene="tadA"
                     /locus_tag="OQM52_001400"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1563313.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44934..45569)
                     /gene="yfhb"
                     /locus_tag="OQM52_001401"
     CDS             complement(44934..45569)
                     /gene="yfhb"
                     /locus_tag="OQM52_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1563314.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45778..46626
                     /locus_tag="OQM52_001402"
     CDS             45778..46626
                     /locus_tag="OQM52_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1563315.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46965..47477)
                     /locus_tag="OQM52_001403"
                     /pseudo
     CDS             complement(46965..47477)
                     /locus_tag="OQM52_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11318 a        13015 c        11947 g        11197 t
ORIGIN      
        1 cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc cccaccagac
       61 ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc cgggtctggc
      121 tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca ggctggcagc
      181 gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag tttaacgccg
      241 cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc catgaaaata
      301 cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt gatgaacctc
      361 gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg acggaactgg
      421 tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt ggtcgcggcg
      481 tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca gccgtggttt
      541 tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc gcatggagtg
      601 aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat tacaggcaat
      661 aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc tttccagcag
      721 gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac tcaccagtaa
      781 ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt atcgacaccg
      841 gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa ttcagacaaa
      901 tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt acttaaacat
      961 gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt atttgttgct
     1021 ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag cgcgtctcag
     1081 cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt attattgttc
     1141 tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg ctggcttaag
     1201 gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat cgcggcaata
     1261 tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa gatacggtgc
     1321 ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa taaccggagt
     1381 ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta agcccgaatg
     1441 ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca cgcaaaaaac
     1501 caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg ctaattcctt
     1561 tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat gtatcggtcg
     1621 ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca atgccaataa
     1681 gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca cgcttcaccg
     1741 gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc ggctctgcga
     1801 cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc agcggatttc
     1861 cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg gtcggcagtt
     1921 tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga acgggcatta
     1981 cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag gtgaaaaaag
     2041 gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca ctatcttcaa
     2101 ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc agtagagtac
     2161 ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg tcttcaagtt
     2221 cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg caaatgcctg
     2281 atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg cagaatttgt
     2341 aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt aacagattag
     2401 ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc ggcgctttac
     2461 ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca acaaagtgcg
     2521 acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg gtattgttca
     2581 gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc agacgccaga
     2641 atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca tcgtcaaaac
     2701 tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg agggtcgccg
     2761 catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg gcaagcacca
     2821 gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga taggtgatga
     2881 tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag aggaacagct
     2941 tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta accgttgccg
     3001 cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg ctgtagccgc
     3061 cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct tctttatcgc
     3121 cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg cgcccgacag
     3181 tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc cgataagtca
     3241 cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt tgcgatggtc
     3301 ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc atcaggcggt
     3361 tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta cgcgtgcctt
     3421 tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag ccttcaccgc
     3481 ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc tggatcagcc
     3541 ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg ctgtaagttg
     3601 ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc atcgcttctg
     3661 tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg cagcccgttc
     3721 attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt ctcgttggat
     3781 gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat atcggtgaat
     3841 cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc aatttcgcgg
     3901 ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca tggttgatag
     3961 tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg cagcttaaac
     4021 ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc tctgagcttt
     4081 ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa gcacaggatt
     4141 tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga atggtttcac
     4201 cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc aaaataacaa
     4261 tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg aaccgactgg
     4321 tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat cgcttgttcg
     4381 atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc attgtcaatg
     4441 acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc gtgcagactt
     4501 tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt tacgtcctgc
     4561 tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt cagaccgtaa
     4621 gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc cgtcacgctc
     4681 aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc agccggtagc
     4741 gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac cagttcgcgc
     4801 tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt ggtgaaatcc
     4861 tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac caggccgccc
     4921 atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc gctgtctggc
     4981 ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac tttatccggg
     5041 tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg caccagcggc
     5101 acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc tacacccgcg
     5161 tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc gatcagttca
     5221 ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt cacggagtcc
     5281 gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg ctgaacgcgg
     5341 ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag cagatggtcg
     5401 aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa cacgccgcga
     5461 cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac agcgatcacg
     5521 ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc gttaagtaag
     5581 cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg ctgggcatcg
     5641 tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag ggcttcagtt
     5701 gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt cagtagcccc
     5761 gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata aggcagatgc
     5821 ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac agaactaatg
     5881 gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga atgcccttgc
     5941 tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc taacgtttcg
     6001 gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc aataccagcc
     6061 gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg taataaggcc
     6121 atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt cggcactgct
     6181 ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt cccacgcctc
     6241 gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt tcacacgttt
     6301 gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca gctcttcgcg
     6361 cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct cgctggcgag
     6421 atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac gcagcgtttg
     6481 ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg ccaggcttcc
     6541 gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca ggctcagtgc
     6601 ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat ccataacatt
     6661 ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc cacgcgcagg
     6721 cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc gcaaccacac
     6781 tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc ttttacgaag
     6841 tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac tttcacgcct
     6901 ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag tacataagcc
     6961 atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt gccgcggtta
     7021 gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga cataaccaaa
     7081 cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg ctttgatcgc
     7141 gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt cttcagcaat
     7201 atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc attcggtgac
     7261 cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg cgtcttcaat
     7321 gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg ccggtgcacc
     7381 caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt tacgcggatt
     7441 ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg attccgatac
     7501 cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt acatttccca
     7561 cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga tggtgtagtc
     7621 gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat gtgccagctc
     7681 gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg acgtacaggc
     7741 ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac cttcaacgta
     7801 gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt tcaggtaaac
     7861 ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca gacgttccat
     7921 ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga cgatctggtg
     7981 aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca tttgcgcttc
     8041 gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg ggccatagat
     8101 tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga ttggcagttt
     8161 gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc acatttcgcc
     8221 gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga tggaaacaag
     8281 gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac cgttacgctg
     8341 cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag tatccagtac
     8401 cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat aaaagttggc
     8461 tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga cgatttcacg
     8521 cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg cttcttcagc
     8581 ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt ccatcgtcat
     8641 gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg agtagtcgag
     8701 ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca aggaatcagg
     8761 ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc tgattctgaa
     8821 ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg tgcgtggcgc
     8881 gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa ccagttcgcc
     8941 taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc acagcgcgtg
     9001 ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg tggcatctac
     9061 agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat ctttgcctaa
     9121 cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac gcagacggga
     9181 aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat cagccaacgg
     9241 taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg catagcgccc
     9301 tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg gggtttttta
     9361 ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag taaattggtc
     9421 aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt ttttatcgtg
     9481 gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg attctgtaca
     9541 ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta ttcggccttg
     9601 ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt caattcctgg
     9661 ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg ccccggatgg
     9721 ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata aaaacgctcc
     9781 agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc attttcctga
     9841 gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat tgccaggttc
     9901 agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt ctgcaactct
     9961 tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt atttgccgct
    10021 tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg cagcgtgcgg
    10081 gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc gagcgtatcg
    10141 acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat cgcctgggag
    10201 tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt tttcatggca
    10261 cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat tcgaatattt
    10321 tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa aacgaagaca
    10381 gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat ccagtgagag
    10441 agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaattta
    10501 attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg cagtaacgat
    10561 ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat tcgtaaatct
    10621 tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac tgatcaggat
    10681 gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg tctgccgcac
    10741 ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat
    10801 cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct gaacggctac
    10861 cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac cggcttcccg
    10921 ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct gttcaacgaa
    10981 tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt cgctgcgggt
    11041 cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc aggcgagctg
    11101 ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa ctatatgctg
    11161 accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc gaacatgcgt
    11221 gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc actcacccgc
    11281 cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc tttcgtcact
    11341 ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa ggttatggca
    11401 ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt tgccttctgc
    11461 gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga taaaatttac
    11521 ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg
    11581 caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc aaccatcatt
    11641 catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag ttggttacct
    11701 gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc aaaaggcact
    11761 ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt gcgccatcgc
    11821 agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt
    11881 cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct cgactccaca
    11941 tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta cttacttgat
    12001 gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct tttactcatt
    12061 cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta tagcctggca
    12121 aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc tttttccgat
    12181 cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc gttgaatccg
    12241 cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt aagacatgct
    12301 aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc caacatttct
    12361 gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct tatcaattat
    12421 gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc tttcggctcc
    12481 ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg ccaccatggc
    12541 aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata tcagcgaaac
    12601 aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg acggtagcta
    12661 ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc tacgacgtgg
    12721 tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga agagtgaact
    12781 gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag cgttagtgaa
    12841 tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt tcctgtgtct
    12901 gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt ttaatccgct
    12961 ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa ttggacgcca
    13021 ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta tggcattact
    13081 tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg acagacaatt
    13141 gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg ttcgttttta
    13201 tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt taaatcgcag
    13261 tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac tgtatccacg
    13321 cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg gcgtccatct
    13381 ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa tgcaggaaaa
    13441 cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc gagaagcgca
    13501 gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc atatgtcggt
    13561 aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg cgccttatac
    13621 catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc tgacaacaca
    13681 tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact ttcgggcgca
    13741 aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg cccagatatt
    13801 gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc gcgacggcag
    13861 agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata taacgcatgg
    13921 cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact atcaaccacg
    13981 gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc gccgagatca
    14041 acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca aagataatga
    14101 cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag tagccagctg
    14161 cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc aataaacaaa
    14221 cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac caaccggtgc
    14281 tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga aagccgagca
    14341 gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca aacatagtga
    14401 ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac agtcgcactt
    14461 tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg gtcaacggta
    14521 ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac gctacgtgcg
    14581 cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg cgcaaggcgg
    14641 aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg agaaaacggg
    14701 caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca atccctttaa
    14761 gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg aaatagccga
    14821 gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag gcgttagtct
    14881 ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct gcttcccgat
    14941 gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat gttgcaaaaa
    15001 taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg atgaaatcgc
    15061 gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg gaaggcacat
    15121 ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata taaccgggta
    15181 tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata ttcgttgcca
    15241 cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg atcggcgcaa
    15301 gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt tgcgcggcgg
    15361 tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact aatggttcgt
    15421 caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt aagccgacgt
    15481 cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc accagctcaa
    15541 tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat aaattcactt
    15601 ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt ttccgcgccc
    15661 gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga aaacattctc
    15721 ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca ccgacacagt
    15781 tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt ttttccgccg
    15841 cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc cgtagttcca
    15901 tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact atttcacata
    15961 ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata catcttatct
    16021 ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac tgattgacac
    16081 ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc aactggagct
    16141 tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga tcccaaaacc
    16201 cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc gccagaaaga
    16261 cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg tgagttatgc
    16321 agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt atggcattaa
    16381 cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt tgtgtaaatc
    16441 ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga tttttggcaa
    16501 ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct ggtatctgga
    16561 tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac aaaagtgggt
    16621 gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt atcatgctct
    16681 gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca gcgataagcg
    16741 cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg cgctgcaatt
    16801 tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg ctaatgtctg
    16861 ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag aacaacgttt
    16921 aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca cgctttcatg
    16981 gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc aagttgaagt
    17041 gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg cttttgaaaa
    17101 cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact cggagaactg
    17161 gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac tgtgtctgga
    17221 aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta ctaactatat
    17281 cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc tgagtagcga
    17341 aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga tgaaatgagt
    17401 gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca cgaagccagt
    17461 ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga gattcgttac
    17521 accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt ccagccgccg
    17581 acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc ccgtctggaa
    17641 acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat cagcaactgc
    17701 caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta tctactttat
    17761 cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga caaagttcgc
    17821 cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga tcaagcggtg
    17881 atcacttccc ataacctgag tgtactgttc tgataaatcg aatttatgcg tgtcccgttg
    17941 cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc gccttgttca
    18001 acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat gcgtcgatgt
    18061 cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc gccagttttt
    18121 gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc acttatccag
    18181 tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca tgagcgatct
    18241 gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg cgctggtcga
    18301 gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg caaaagtcgc
    18361 cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg tgacctgtta
    18421 tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc tggattgttt
    18481 tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc ccgcagagac
    18541 gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc tgctgggcgc
    18601 aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca cactgtcaaa
    18661 tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac atgccgcaac
    18721 cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga atggcgtcgg
    18781 cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa gtgaaacctc
    18841 gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc aatttttccc
    18901 gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca ataatcgcgc
    18961 attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca ttcgccacgt
    19021 agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca ttattgtcgg
    19081 tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt tcaccggtga
    19141 gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac tctcgaaatc
    19201 catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact ggtggcagga
    19261 aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg acacacgaga
    19321 gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag caaccggcgc
    19381 ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta ccctacacca
    19441 tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag tcgtgattgt
    19501 cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca gcgcagcgca
    19561 gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc gtaatgcacc
    19621 accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc gcattctgct
    19681 caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc tgcaaagtgg
    19741 ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca acgagcaact
    19801 ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg cttgccgcac
    19861 ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg ataatggtgc
    19921 actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg ccgctgccgc
    19981 aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg atcagtacag
    20041 tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc gtggcaaccc
    20101 ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg gtgcggtcac
    20161 gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg gcaaaacgtt
    20221 tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat caggataatt
    20281 agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta cgtttaaatc
    20341 acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt ttggagctgc
    20401 ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca ttatttttgg
    20461 ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt tgggcgcgcc
    20521 aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg cgatattaat
    20581 cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca cgctgggtac
    20641 ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc caaatatgat
    20701 taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa ttaccggacc
    20761 gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg aacgccttat
    20821 ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg ttgttgattc
    20881 ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat ccggcgcatg
    20941 gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg cagggtcgga
    21001 aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg cataaccttc
    21061 ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt tcacccactg
    21121 acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct cgcaaaactc
    21181 acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct gaatccgcgt
    21241 ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg tctctgcccc
    21301 ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa acgcctgact
    21361 tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac tatcatcgct
    21421 gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt gcgcatccca
    21481 ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt tgccgctcac
    21541 ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac cgcttttttt
    21601 accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc cgccctggtc
    21661 agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc gctaactcct
    21721 taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag cacataggca
    21781 tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca cagcttccag
    21841 tcagtcaaat cgtgggcgca tttttccata tggaacagac tggtcaccca ggagccgata
    21901 atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt tccggtttca
    21961 ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa gcgacctgcg
    22021 gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc agcaattatc
    22081 tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa ctgttttgcc
    22141 atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc gcgacctttc
    22201 gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac gttatcgctg
    22261 ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca actgataaac
    22321 gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc ttccgccagc
    22381 agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc tttgccagta
    22441 caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga aatgtgatac
    22501 accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc cacaatctgc
    22561 ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg tgccgccgcc
    22621 gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc ggaggatttc
    22681 attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg cagatcgacg
    22741 gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc attaacaata
    22801 cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag cgtttcgctt
    22861 tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg atgacgccgc
    22921 gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta ccgattagcg
    22981 taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg gaaagtgccg
    23041 taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg gcacctgcgt
    23101 taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat aatcccgaaa
    23161 gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc gccgccgtgg
    23221 cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta atgaacacaa
    23281 acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga aattgtccgc
    23341 ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg gtcataaaca
    23401 gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt ggtacgttaa
    23461 acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc aacaccagca
    23521 gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac accgagacaa
    23581 aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc agcgtcatcg
    23641 cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg ttattcaatg
    23701 agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc accaccagca
    23761 aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc ttgccctgcg
    23821 gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat cagtgcacag
    23881 acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc tgcgagaacg
    23941 tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc acttcactgg
    24001 agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg taaatctgct
    24061 gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc tggctggcgg
    24121 catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc gtgccgatgg
    24181 gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg gtcaggcggc
    24241 ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg gtcagcactg
    24301 tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt tctggcgtat
    24361 agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc tcgccgttga
    24421 taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc aattcactgc
    24481 gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc gtaaagctga
    24541 tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc tgatccacaa
    24601 tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt agcgacacaa
    24661 tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca tcgcgcataa
    24721 cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca taaatcaccg
    24781 ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact tctgcactgg
    24841 caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc atcacccgtg
    24901 caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt tcaggactaa
    24961 cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc aggtaatcaa
    25021 tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc gtcagccctt
    25081 ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca cggcgagtca
    25141 acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg ctatccgggc
    25201 gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt ttacctaaca
    25261 gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca acgacgccgg
    25321 ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt gccgtgaaca
    25381 tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc cagccactgt
    25441 ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt ttcaccgtcg
    25501 gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat tttcttaccg
    25561 gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc aatttcggtt
    25621 gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg taccgctttt
    25681 accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc cggcgtggag
    25741 ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc ctggttagcg
    25801 acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt acgtcgctgc
    25861 acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc aattttattg
    25921 gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt ggatcgccga
    25981 ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg ttgtagcaaa
    26041 tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca ttttcagaca
    26101 ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca aaggtacttt
    26161 ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact gaagaatctt
    26221 ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta tcaaggtata
    26281 ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta cctaatagcg
    26341 tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg cctcctgtag
    26401 ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc gcgtgccgct
    26461 caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc atcacagtgg
    26521 tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg cgagcagact
    26581 catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc aggcattttt
    26641 ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt gaaacagcaa
    26701 agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc atttcactaa
    26761 acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct tgccagaaga
    26821 gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga tcgccggtcg
    26881 ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg gcgcatatcg
    26941 cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg cttaaattct
    27001 ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct ctggcatcaa
    27061 gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc ttcccttccc
    27121 cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg tctgcctgac
    27181 ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc agcgcaattt
    27241 ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt aacttatcca
    27301 gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa gcattatcaa
    27361 gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc gctaacccgc
    27421 gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag gcaatggctt
    27481 tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca aaccaacact
    27541 cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg acaaactgcg
    27601 ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc agcaagctgg
    27661 cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc tctgccgcca
    27721 gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta acgtcgcgtc
    27781 cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg aaccagttca
    27841 gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc tgcaaacgcg
    27901 cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc acttcctgat
    27961 tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg aagttatcat
    28021 tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg ccgctccaga
    28081 ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg
    28141 cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt gcacgtttca
    28201 gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat tccagcatcg
    28261 ccagcgccag gttacagcga taatccagcg ggtccagcgc cacgccgcgc aggtagtaat
    28321 cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt
    28381 catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc ggcaacggca
    28441 actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca tcggagagtt
    28501 caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg gtcgctggtg
    28561 tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg atcgccagac
    28621 ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc tcactacgct
    28681 ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa tgataaggca
    28741 ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcaggctggt
    28801 tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg gtcagactct
    28861 tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta cctggcgcaa
    28921 tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac gcgccgacaa
    28981 aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt ttatagcgag
    29041 aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag
    29101 cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg aaaacgctct
    29161 gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga cgcggcgtgg
    29221 cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg cgcagggtga
    29281 aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac accgtctgtg
    29341 caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatggt
    29401 gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcatc cccaccagcg
    29461 caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta tcccatgcgc
    29521 gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc
    29581 acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg
    29641 gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg ggatgtatat
    29701 cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag
    29761 tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtgtattg
    29821 aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa
    29881 gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga
    29941 actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cgcaaatttc
    30001 gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat
    30061 tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg cgccggaagg
    30121 tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc
    30181 ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga cgccgcaggc
    30241 gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac
    30301 catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg tatcgcaaac
    30361 catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg aagagaagct
    30421 gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca
    30481 aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc
    30541 gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac agattggtca
    30601 caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca gcctggaaac
    30661 cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt
    30721 taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg cctggcagag
    30781 tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg gtttgagtct
    30841 gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc
    30901 ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg
    30961 cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca
    31021 acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga tttgatggcg
    31081 cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa aaattgcctg
    31141 atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg caagattttg
    31201 taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat tgtcagcaaa
    31261 tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt
    31321 gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca tccagccagc
    31381 cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag tacctacacg
    31441 aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt tgttgacggt
    31501 gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt tatcaaccag
    31561 atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc cgcgctcgag
    31621 gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag ttttgaactc
    31681 aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca tcaacggacc
    31741 gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt cgctaccacc
    31801 tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac
    31861 aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg
    31921 cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca ttttcgccca
    31981 gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct tgtgttcttt
    32041 ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac cgtaaggaac
    32101 gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt gaccgccatg
    32161 cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt agaccgcaaa
    32221 gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga acagttcttt
    32281 cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg
    32341 tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg cctgcattac
    32401 gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct gacgtacttt
    32461 ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca tttcacgctt
    32521 taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat aggcggtcct
    32581 gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct tgacaaagga
    32641 atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag agcttgcaac
    32701 cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca cattctttct
    32761 gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg catttgagat
    32821 acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc atcgctacag
    32881 taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc catttctacg
    32941 accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt aaccagcgta
    33001 atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt aatattgaaa
    33061 acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc agcttccaga
    33121 tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg gacgaaatgt
    33181 tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta ctggctaatg
    33241 tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc ggtggttggg
    33301 aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt atcaccagct
    33361 tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg caatatctcg
    33421 gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac tctttgactc
    33481 gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt gggcaggtat
    33541 ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc gctccggcag
    33601 gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct accggtgttg
    33661 gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac acagcacaag
    33721 tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat gaagttaagg
    33781 aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag
    33841 ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc aaactggaag
    33901 gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc ttcatgcagt
    33961 ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat tacgaatgct
    34021 ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg gcaacccttg
    34081 acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg acccgagcca
    34141 cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg
    34201 tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc actttcggca
    34261 gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc tggcgaccaa
    34321 agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt gcttcgcgga
    34381 catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta ttccttgaaa
    34441 aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat tccttgaaat
    34501 cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg
    34561 ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta cgcaagtagt
    34621 tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt tcaccctcca
    34681 gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc acgcactgtt
    34741 caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc aggcgtttca
    34801 tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca
    34861 ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga attttcaggc
    34921 tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc atcgcttttg
    34981 acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg cggttactgc
    35041 ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt aacggcgcag
    35101 gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg aataaacctt
    35161 cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc tccgactcca
    35221 gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg ttgcgcgggc
    35281 tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga ccgttaatca
    35341 aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg
    35401 ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg gattgctcca
    35461 gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg aggaaactaa
    35521 aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca gtaagaataa
    35581 ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata ccgtccattt
    35641 catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt acccgtaatc
    35701 cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg cgcaggccaa
    35761 gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt ttatggctca
    35821 tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg cgtatccggc
    35881 gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt caggttttcc
    35941 agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg gcgcaatgta
    36001 tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc ttccgccagt
    36061 tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac ctgtgccggg
    36121 atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata cggcgtaatt
    36181 ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc gtcgtcatat
    36241 tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc catcgcccgc
    36301 agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat gtcgctacat
    36361 tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc tggcgtatca
    36421 atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc
    36481 agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac cagacttaaa
    36541 gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg cggagaaacc
    36601 ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac tgacaaataa
    36661 aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac ggtaattcaa
    36721 tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct tgcatacggc
    36781 gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc ccttttcgct
    36841 ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggcg
    36901 tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg cgaaggcaaa
    36961 tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc agtacgctca
    37021 tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg gtatgcatca
    37081 ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc ggtgctaatt
    37141 caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca agcagttgtt
    37201 cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc tctttttgct
    37261 ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg cgcatactcg
    37321 ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt tgggattcca
    37381 gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt aactcgctcg
    37441 gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg ttgatcatgc
    37501 gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc atcaccagac
    37561 tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg gcgatttcac
    37621 gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat
    37681 tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca ctgttgttac
    37741 actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt ttatcgtctg
    37801 gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg ctttgataaa
    37861 ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc tccatctcca
    37921 gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg cggttaacca
    37981 gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc agcaccaaca
    38041 ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag cggggaaaaa
    38101 cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg
    38161 ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat aaggcggtgc
    38221 ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa gttggacggc
    38281 aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg gctgacataa
    38341 gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa gaaaatagtt
    38401 tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc tatttgaatg
    38461 taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt agattgcaat
    38521 aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat tgtcgtgatt
    38581 tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt gcttaatgcc
    38641 gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac gcgcattgcg
    38701 gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg agttgctgac
    38761 agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac tttcagtcgt
    38821 aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt cagtgacttt
    38881 gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg ccgccagatg
    38941 cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg gcatttgcga
    39001 gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca ggctgaaacg
    39061 cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac tccccgggat
    39121 cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg ccagcgtttg
    39181 cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca ggatagactt
    39241 cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc acgcgaccag
    39301 ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat gcacgtcgat
    39361 agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt tcacgccctg
    39421 ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg ctgccacatc
    39481 ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt tagatttcgc
    39541 ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct gccaggtagt
    39601 ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct gcccgttggc
    39661 ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc aatccgcaag
    39721 cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa cctgaatcac
    39781 cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag aggcgatatc
    39841 cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca gcaggcctcc
    39901 atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct gaatcgcgtc
    39961 atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc caagctgacg
    40021 ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca aatcaatcag
    40081 cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta catcttccac
    40141 gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct cttcgttacc
    40201 ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg tcaggcccag
    40261 cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg catcttcacc
    40321 agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga tatcgccaat
    40381 ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag aggcggcgaa
    40441 atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt agtagctgtc
    40501 gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc ccaccatctg
    40561 atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga aagttttctc
    40621 cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga tcccttcacg
    40681 gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct tcggcgtttt
    40741 accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac gatcgtgcag
    40801 agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc gctcacgctt
    40861 acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt agcgttcctg
    40921 ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta gaatatcgcg
    40981 cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg cgttagaaag
    41041 accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa gctgccagca
    41101 acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct gtacggaagc
    41161 aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc cgccgccaag
    41221 accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt tgatctcgcc
    41281 tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga tcggtttgtg
    41341 ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag tacggaagta
    41401 gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca gcgggccttc
    41461 ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt cttcccacgg
    41521 ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac cagctttcgg
    41581 cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac cggtcgccgc
    41641 gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca tcaggatgtg
    41701 cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac
    41761 ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt gatctggcgt
    41821 ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt caccgtcgat
    41881 aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct gagcgaacat
    41941 atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct gcagatagtc
    42001 aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca gcgcctgacg
    42061 gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg caaacaactg
    42121 ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca gttcagcggt
    42181 aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag caacgccgcg
    42241 ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg ctttcgaaga
    42301 ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt gcggggcgtg
    42361 gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt
    42421 taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt gaaccgggag
    42481 cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca gtgcaggcga
    42541 accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt acccaagggg
    42601 gggggaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat
    42661 aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg
    42721 gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacgttt
    42781 tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat
    42841 tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc
    42901 tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg
    42961 agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt tggcctggat
    43021 tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg
    43081 cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct gctggcggca
    43141 ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac ctattattcc
    43201 gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct gggcaacctg
    43261 acggcggagc aactcaccgt tgcaccgggt catgtggtag ttaacgatct ccagaccctg
    43321 aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa
    43381 ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc
    43441 agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac cgatgaacaa
    43501 ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac
    43561 ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa atacctgggg
    43621 catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta
    43681 ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg
    43741 gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc gacgggtgtg
    43801 cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc
    43861 gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat gagtaaagtg
    43921 ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta caatatgggc
    43981 tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt
    44041 tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg
    44101 acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat tcgtaagtat
    44161 cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga agcggcgatg
    44221 caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga gaaatttcct
    44281 tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc ctctctgctg
    44341 ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg cgcttttttc
    44401 tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca
    44461 tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg atgcagcaca
    44521 tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca
    44581 atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt gacatacaac
    44641 gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag ggccatgatt
    44701 tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc gccgattacc
    44761 cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca ggcacgtttc
    44821 gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc agacaaaaag
    44881 aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt actctattcc
    44941 agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa ataaagcagc
    45001 ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt gccgattttg
    45061 cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac ccaaccacca
    45121 tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa
    45181 taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc agcatcggaa
    45241 cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcgcga
    45301 aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg
    45361 cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca
    45421 atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg gcgacgcagt
    45481 aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa
    45541 aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat
    45601 taattttcgc tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc
    45661 gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt
    45721 cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag gaaagatatg
    45781 aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga taaaaaactg
    45841 gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc
    45901 aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa
    45961 ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc
    46021 tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa
    46081 ctgattaaag aaaataccgc cgctatgtac gcaacgctaa acgttaatag tgaagagaaa
    46141 ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac cggtattggc
    46201 gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct
    46261 gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat
    46321 ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc ggcagatgag
    46381 atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa tgccctgcaa
    46441 cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca
    46501 atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc gctgattcag
    46561 caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg
    46621 gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct
    46681 tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa tcaaataaaa
    46741 acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt ggatttatgg
    46801 tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt taacgtcctc
    46861 cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa cattaacttt
    46921 tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat aaccctggct
    46981 ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac cttctggcga
    47041 gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga aagtttggga
    47101 cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt tgttcgttca
    47161 acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa aaagcgtccc
    47221 attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc tcgttcgcgc
    47281 aacgcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc cagcttccag
    47341 acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt ttttcagccc cggtctttcg
    47401 gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca gttcaacgca
    47461 ttacgttgta gatcggt
//
LOCUS       DAJVQR010000024        45756 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVQR010000024 DAJVQR010000000
VERSION     DAJVQR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            95..2248
                     /locus_tag="OQM52_001404"
     CDS             95..2248
                     /locus_tag="OQM52_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1563316.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="OQM52_001405"
     CDS             2301..4031
                     /locus_tag="OQM52_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563317.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OQM52_001406"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OQM52_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1563318.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="OQM52_001407"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="OQM52_001407"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1563319.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="OQM52_001408"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="OQM52_001408"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1563320.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OQM52_001409"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OQM52_001409"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1563321.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="OQM52_001410"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="OQM52_001410"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1563322.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="OQM52_001411"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="OQM52_001411"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1563323.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="OQM52_001412"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="OQM52_001412"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1563324.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="OQM52_001413"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="OQM52_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1563325.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OQM52_001414"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OQM52_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1563326.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OQM52_001415"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OQM52_001415"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1563327.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OQM52_001416"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OQM52_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1563328.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OQM52_001417"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OQM52_001417"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1563329.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OQM52_001418"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OQM52_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1563330.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OQM52_001419"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OQM52_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1563331.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="OQM52_001420"
     CDS             16289..16828
                     /locus_tag="OQM52_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1563332.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="OQM52_001421"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="OQM52_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1563333.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="OQM52_001422"
     CDS             complement(18187..18534)
                     /locus_tag="OQM52_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1563334.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OQM52_001423"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OQM52_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1563335.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OQM52_001424"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OQM52_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1563336.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OQM52_001425"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OQM52_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1563337.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OQM52_001426"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OQM52_001426"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1563338.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OQM52_001427"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OQM52_001427"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1563339.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="OQM52_001428"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="OQM52_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OQM52_001429"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OQM52_001429"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS1563340.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OQM52_001430"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OQM52_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1563341.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OQM52_001431"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OQM52_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1563342.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OQM52_001432"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OQM52_001432"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1563343.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OQM52_001433"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OQM52_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1563344.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="OQM52_001434"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="OQM52_001434"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1563345.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="OQM52_001435"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="OQM52_001435"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1563346.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="OQM52_001436"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="OQM52_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1563347.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OQM52_001437"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OQM52_001437"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1563348.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="OQM52_001438"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="OQM52_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1563349.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="OQM52_001439"
     CDS             complement(39992..40447)
                     /locus_tag="OQM52_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563350.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="OQM52_001440"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="OQM52_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OQM52_001441"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OQM52_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1563351.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="OQM52_001442"
     CDS             complement(41290..41967)
                     /locus_tag="OQM52_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1563352.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OQM52_001443"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OQM52_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1563353.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OQM52_001444"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OQM52_001444"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1563354.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="OQM52_001445"
     CDS             complement(43173..43631)
                     /locus_tag="OQM52_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1563355.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="OQM52_001446"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="OQM52_001446"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1563356.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="OQM52_001447"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="OQM52_001447"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1563357.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPLPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11896 a        11933 c        11196 g        10731 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcag cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtga
//
LOCUS       DAJVQR010000025        43210 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVQR010000025 DAJVQR010000000
VERSION     DAJVQR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OQM52_001448"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OQM52_001448"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS1563358.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OQM52_001449"
     CDS             complement(1672..2730)
                     /locus_tag="OQM52_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1563359.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAYRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OQM52_001450"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OQM52_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OQM52_001451"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OQM52_001451"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS1563360.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OQM52_001452"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OQM52_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1563361.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OQM52_001453"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OQM52_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1563362.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OQM52_001454"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OQM52_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1563363.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OQM52_001455"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OQM52_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1563364.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OQM52_001456"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OQM52_001456"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1563365.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OQM52_001457"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OQM52_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1563366.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OQM52_001458"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OQM52_001458"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1563367.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OQM52_001459"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OQM52_001459"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1563368.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OQM52_001460"
     CDS             complement(17699..18322)
                     /locus_tag="OQM52_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1563369.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OQM52_001461"
     CDS             18619..18894
                     /locus_tag="OQM52_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563370.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OQM52_001462"
     CDS             complement(18971..19213)
                     /locus_tag="OQM52_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1563371.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OQM52_001463"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OQM52_001463"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1563372.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OQM52_001464"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OQM52_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1563373.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OQM52_001465"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OQM52_001465"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1563374.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OQM52_001466"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OQM52_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OQM52_001467"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OQM52_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1563375.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OQM52_001468"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="OQM52_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OQM52_001469"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OQM52_001469"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1563376.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OQM52_001470"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OQM52_001470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1563377.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OQM52_001471"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OQM52_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="OQM52_001472"
     CDS             complement(30355..31602)
                     /locus_tag="OQM52_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1563378.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="OQM52_001473"
     CDS             complement(31624..31950)
                     /locus_tag="OQM52_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1563379.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OQM52_001474"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OQM52_001474"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1563380.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="OQM52_001475"
     CDS             33338..34594
                     /locus_tag="OQM52_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1563381.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="OQM52_001476"
     CDS             34709..35035
                     /locus_tag="OQM52_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1563382.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="OQM52_001477"
     CDS             complement(35176..36414)
                     /locus_tag="OQM52_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1563383.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="OQM52_001478"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="OQM52_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1563384.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="OQM52_001479"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="OQM52_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="OQM52_001480"
     tRNA            complement(40322..40397)
                     /locus_tag="OQM52_001480"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="OQM52_001481"
     tRNA            complement(40437..40512)
                     /locus_tag="OQM52_001481"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="OQM52_001482"
     CDS             40733..41092
                     /locus_tag="OQM52_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1563385.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="OQM52_001483"
     CDS             41094..41486
                     /locus_tag="OQM52_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1563386.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OQM52_001484"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OQM52_001484"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1563387.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT        11510 a        10756 c        10008 g        10936 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg taggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg
//
LOCUS       DAJVQR010000026        42791 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVQR010000026 DAJVQR010000000
VERSION     DAJVQR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(90..626)
                     /gene="ssb1"
                     /locus_tag="OQM52_001485"
     CDS             complement(90..626)
                     /gene="ssb1"
                     /locus_tag="OQM52_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1563388.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            880..3702
                     /gene="uvrA"
                     /locus_tag="OQM52_001486"
     CDS             880..3702
                     /gene="uvrA"
                     /locus_tag="OQM52_001486"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1563389.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3737..4093)
                     /locus_tag="OQM52_001487"
     CDS             complement(3737..4093)
                     /locus_tag="OQM52_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1563390.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4097..4513)
                     /locus_tag="OQM52_001488"
     CDS             complement(4097..4513)
                     /locus_tag="OQM52_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1563391.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4624..5337)
                     /gene="aphA"
                     /locus_tag="OQM52_001489"
     CDS             complement(4624..5337)
                     /gene="aphA"
                     /locus_tag="OQM52_001489"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1563392.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5739..5943
                     /locus_tag="OQM52_001490"
                     /pseudo
     CDS             5739..5943
                     /locus_tag="OQM52_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6446..7639)
                     /gene="tyrB"
                     /locus_tag="OQM52_001491"
     CDS             complement(6446..7639)
                     /gene="tyrB"
                     /locus_tag="OQM52_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1563393.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7892..8971)
                     /gene="alr"
                     /locus_tag="OQM52_001492"
     CDS             complement(7892..8971)
                     /gene="alr"
                     /locus_tag="OQM52_001492"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1563394.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9024..10439)
                     /gene="dnaB"
                     /locus_tag="OQM52_001493"
     CDS             complement(9024..10439)
                     /gene="dnaB"
                     /locus_tag="OQM52_001493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1563395.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10522..11505
                     /locus_tag="OQM52_001494"
     CDS             10522..11505
                     /locus_tag="OQM52_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1563396.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11671..11913)
                     /gene="pspG"
                     /locus_tag="OQM52_001495"
     CDS             complement(11671..11913)
                     /gene="pspG"
                     /locus_tag="OQM52_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1563397.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12047..13084)
                     /gene="dusA"
                     /locus_tag="OQM52_001496"
     CDS             complement(12047..13084)
                     /gene="dusA"
                     /locus_tag="OQM52_001496"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1563398.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEVFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13447..14432)
                     /locus_tag="OQM52_001497"
                     /pseudo
     CDS             complement(13447..14432)
                     /locus_tag="OQM52_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14750..15265
                     /gene="zur"
                     /locus_tag="OQM52_001498"
     CDS             14750..15265
                     /gene="zur"
                     /locus_tag="OQM52_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1563399.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15307..15516)
                     /locus_tag="OQM52_001499"
     CDS             complement(15307..15516)
                     /locus_tag="OQM52_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1563400.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15632..16957)
                     /gene="dinF"
                     /locus_tag="OQM52_001500"
     CDS             complement(15632..16957)
                     /gene="dinF"
                     /locus_tag="OQM52_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1563401.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17030..17638)
                     /gene="lexA"
                     /locus_tag="OQM52_001501"
     CDS             complement(17030..17638)
                     /gene="lexA"
                     /locus_tag="OQM52_001501"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1563402.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17748..18116)
                     /gene="dgkA"
                     /locus_tag="OQM52_001502"
     CDS             complement(17748..18116)
                     /gene="dgkA"
                     /locus_tag="OQM52_001502"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1563403.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18287..20710
                     /gene="plsB"
                     /locus_tag="OQM52_001503"
     CDS             18287..20710
                     /gene="plsB"
                     /locus_tag="OQM52_001503"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1563404.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20865..21737)
                     /gene="ubiA"
                     /locus_tag="OQM52_001504"
     CDS             complement(20865..21737)
                     /gene="ubiA"
                     /locus_tag="OQM52_001504"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1563405.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21750..22247)
                     /gene="ubiC"
                     /locus_tag="OQM52_001505"
     CDS             complement(21750..22247)
                     /gene="ubiC"
                     /locus_tag="OQM52_001505"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1563406.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22470..24050)
                     /locus_tag="OQM52_001506"
                     /pseudo
     CDS             complement(22470..24050)
                     /locus_tag="OQM52_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24279..25199)
                     /gene="malM"
                     /locus_tag="OQM52_001507"
     CDS             complement(24279..25199)
                     /gene="malM"
                     /locus_tag="OQM52_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS1563407.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25351..26691)
                     /gene="lamB"
                     /locus_tag="OQM52_001508"
     CDS             complement(25351..26691)
                     /gene="lamB"
                     /locus_tag="OQM52_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS1563408.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26763..27878)
                     /gene="malK"
                     /locus_tag="OQM52_001509"
     CDS             complement(26763..27878)
                     /gene="malK"
                     /locus_tag="OQM52_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS1563409.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28243..29433
                     /gene="malE"
                     /locus_tag="OQM52_001510"
     CDS             28243..29433
                     /gene="malE"
                     /locus_tag="OQM52_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS1563410.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29587..31131
                     /gene="malF"
                     /locus_tag="OQM52_001511"
     CDS             29587..31131
                     /gene="malF"
                     /locus_tag="OQM52_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS1563411.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31146..32036
                     /gene="malG"
                     /locus_tag="OQM52_001512"
     CDS             31146..32036
                     /gene="malG"
                     /locus_tag="OQM52_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS1563412.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32407..33882
                     /gene="xylE"
                     /locus_tag="OQM52_001513"
     CDS             32407..33882
                     /gene="xylE"
                     /locus_tag="OQM52_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS1563413.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33926..34336)
                     /gene="psiE"
                     /locus_tag="OQM52_001514"
     CDS             complement(33926..34336)
                     /gene="psiE"
                     /locus_tag="OQM52_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1563414.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34462..34605)
                     /locus_tag="OQM52_001515"
     CDS             complement(34462..34605)
                     /locus_tag="OQM52_001515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563415.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34606..34884
                     /locus_tag="OQM52_001516"
     CDS             34606..34884
                     /locus_tag="OQM52_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563416.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34931..37027)
                     /locus_tag="OQM52_001517"
     CDS             complement(34931..37027)
                     /locus_tag="OQM52_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1563417.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37027..37764)
                     /locus_tag="OQM52_001518"
     CDS             complement(37027..37764)
                     /locus_tag="OQM52_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1563418.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37761..38399)
                     /locus_tag="OQM52_001519"
     CDS             complement(37761..38399)
                     /locus_tag="OQM52_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1563419.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38513..38755)
                     /locus_tag="OQM52_001520"
     CDS             complement(38513..38755)
                     /locus_tag="OQM52_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563420.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39218..40867)
                     /gene="pgi"
                     /locus_tag="OQM52_001521"
     CDS             complement(39218..40867)
                     /gene="pgi"
                     /locus_tag="OQM52_001521"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1563421.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41392..42741
                     /gene="lysC"
                     /locus_tag="OQM52_001522"
     CDS             41392..42741
                     /gene="lysC"
                     /locus_tag="OQM52_001522"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1563422.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10643 a        11016 c        10504 g        10628 t
ORIGIN      
        1 gcctccataa cggaggcttc ttttttatgg ctggcagatg ctttaatcat ccaccttaaa
       61 acaatataac ctattgtttt aatgacaaat cagaacggaa tgtcatcatc aaagtccatc
      121 ggcggctcgt tagacggcgc tgccggagcg gactgctgcg ggcgagactg cgcgccgccg
      181 ctgaactgat tgccaccctg cggctgctga ggctgacccc aaccgccctg cggctgacca
      241 ccaccgatat tgccacctgc cggagcgcca ccaccctgac gaccacccag catctgcatg
      301 gtgccgccaa cgttcaccac gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc
      361 catttacggg tacgcagctg accttcgata taaacctgag aacctttacg cagatattcg
      421 ctcgccactt ctgccagttt gccgaacagc acaacgcggt gccattcagt ctgttctttc
      481 atctcgccgg tcgctttatc acgccaggat tcggaagtag ccagcgtaat gttggcaact
      541 gcgccaccat ttggcatgta gcgtacttcc gggtcctgac ccagattacc aacgagaata
      601 accttgttta cgcctctgct ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa
      661 gtgtaaacgc gcgattgtac cattaccaat agcgctttta ctatgttgtg acctcggttc
      721 cgggaaacaa acctggccag acattgttac acaacactcc ggataatgca ttccaatact
      781 gtatattcat tcaggtcaat ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca
      841 tgccaccggg caaaaaagcg tttaatccgg gaaaggtgaa tggataagat cgaagttcgg
      901 ggcgcccgca cccataatct caaaaacatc aacctcgtta tcccccgcga caagctcatt
      961 gtcgtgaccg ggctttcggg ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc
     1021 gaagggcagc gccgttacgt tgaatccctt tccgcctacg cgcggcagtt tctgtcactg
     1081 atggaaaagc cggacgtcga tcatattgag gggctttctc ctgccatctc aattgagcag
     1141 aaatcgacgt ctcataaccc gcgttctacg gtggggacaa tcaccgaaat ccacgactat
     1201 ttgcgtttgt tgttcgcccg cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg
     1261 gcggcgcaaa ccgtcagcca gatggtggat aacgtgctgt cgcagccgga aggcaagcgt
     1321 ctgatgctgc tcgcgccaat cattaaagag cgcaaaggcg aacacaccaa aacgctggag
     1381 aacctggcaa gccagggtta catccgtgct cgtattgatg gcgaagtctg cgatctttcc
     1441 gatccgccga aactggaact gcaaaagaaa cataccattg aagtggtggt tgatcgcttc
     1501 aaggtgcgtg acgatcttac ccaacgtctt gcggagtcgt ttgaaaccgc gctggaactt
     1561 tccggtggta cagcggtagt ggcggatatg gacgacccga aagcggaaga gctgctgttc
     1621 tccgccaact tcgcctgccc aatttgcggc tacagtatgc gtgaactgga gccgcgactg
     1681 ttttcgttta acaacccggc aggtgcctgc ccgacctgtg acggccttgg cgtacagcaa
     1741 tatttcgatc ctgaccgcgt gatccaaaac cccgagctgt cactggctgg cggtgcgatc
     1801 cgtggctggg atcgccgcaa cttctattac ttccagatgc tgaaatcgct ggcagatcac
     1861 tataagttcg acgtcgaagc gccgtggggc agcctgagcg cgaacgtgca taaagtggtg
     1921 ttgtacggtt ctggcaaaga aaacattgaa ttcaaataca tgaacgatcg tggcgatacc
     1981 tccatccgtc gtcatccgtt cgaaggcgtg ctgcacaata tggagcgccg ttataaagag
     2041 acagaatcca gtgcggtacg tgaagaatta gccaagttta tcagcaatcg cccatgcgcc
     2101 agctgcgaag ggacgcgtct gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg
     2161 ctgcctgcta tctccgacat gagcatcggt catgcgatgg aattcttcaa caatctcaaa
     2221 ctcgctggtc agcgggcgaa gattgcggaa aaaattctta aagagatcgg cgatcgcctg
     2281 aaattcctcg taaacgtcgg cctgaattac ctgacacttt cccgctcggc agaaacactt
     2341 tccggcggtg aagcccagcg tatccgtctg gcgagccaga ttggtgcggg cctggttggc
     2401 gttatgtacg tgctggacga gccgtctatc ggcctgcacc agcgcgataa cgagcgcctg
     2461 ttgggtacgc ttatccatct gcgcgatctc ggtaataccg tgattgtggt ggagcatgac
     2521 gaagacgcaa ttcgcgccgc tgaccatgtg atcgatatcg gtcctggtgc gggtgttcac
     2581 ggcggtgaag tggtcgcaga aggtccgctg gaagcgatta tggcggtgcc tgaatcgttg
     2641 accgggcagt acatgagcgg taaacgcaag attgaagtgc cgaagaaacg cgttccggcg
     2701 aatccagaaa aagtgctgaa gctgacaggc gcacgcggca acaacctgaa ggacgtgacg
     2761 ctcacgctgc cagtcggtct gtttacctgc atcacagggg tttcaggttc cggtaaatcg
     2821 acgctgatta acgacacact gttcccgatt gcccaacgcc agttgaatgg tgcgaccatc
     2881 gccgaaccgg caccgtatcg cgatattcag ggactggagc atttcgataa agtgatcgat
     2941 atcgaccaaa gcccaattgg tcgtactcca cgttctaacc cggcgaccta taccggcgtg
     3001 tttacgcctg tgcgcgaact gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg
     3061 ccaggacgtt tcagctttaa cgtccgtggc gggcgctgcg aagcctgtca gggcgacggt
     3121 gtgatcaaag tggagatgca cttcctgccg gatatctacg tgccgtgcga tcagtgcaaa
     3181 ggtaaacgct ataaccgtga aacgctggaa attaagtaca aaggcaaaac catccacgaa
     3241 gtgctggata tgaccatcga agaggcgcgt gagttctttg atgcggtgcc agctctggcg
     3301 cgtaagctgc aaacgttgat ggacgttggt ctgacgtaca ttcgcctggg gcagtccgca
     3361 accacacttt ctggtggtga agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc
     3421 ggcaccgggc agacgctgta tattctcgac gagccgacca ccggtctgca cttcgccgat
     3481 attcagcaac tcctcgacgt gctgcataaa ctgcgcgatc agggcaatac cattgtggtg
     3541 attgagcaca atctcgacgt gatcaaaacc gctgactgga ttgtcgacct gggaccagaa
     3601 ggcggcagtg gcggcggcga aatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc
     3661 gaagcttcgc atacggcacg cttcctcaag ccgatgctgt aatcgttaag gccgctttct
     3721 gagcggcctt ttcctttcag agttgtacca gcaatttacg cttttcttcc ggtagtaaat
     3781 tcactgcctg ctgataagac gcatccacca gataatagat ttgcgaatcc ggcagcgaac
     3841 cgtcgagata cacggtgctc cagtgcgctt tattcagatg gcggctagga cgcacatcgc
     3901 tgtgctgctg acgtagcagc tccgccagct ccgggctggt tttcagcgaa acagctgggc
     3961 gattttctac ttctttcacc atcgcaaaca gtacatcttc cactttgatc tgcgtcgctt
     4021 tccagtcgtt atgcacgctc tgttctgcgc ttggttttgc catgcaatat tgtagcaact
     4081 ccgaaatggt cattttttac tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt
     4141 ggatgcgatg ttcccccagc cagatgcctt gccaagtgcc ggtctgaata cgccctttat
     4201 gtaccggcaa tacaagcgat gttcccagca ttgaggattt gatatgagaa ggcatatcgt
     4261 ctgctccctc atagtcatgc tcataatttc cgttgtcggg aacggtgcgg aggaaaaaac
     4321 actccatgtc gtggcgtacg gtgggatcgc agttctcatt aagtgtcaga gaggcggagg
     4381 tatgttgcag caacagatgc agtaagccga tgttaacgcg cggcatatca gccagctgat
     4441 tcagaatttc atccgttacc agatgaaacc cacgagattt ggcgctaagc gtgagcgtct
     4501 tttgatacca catgtgctgc tccttgataa aactctctta atcagtttgc agcaagacag
     4561 cgaaaggata aaggtgtgat taaaaaaaca gcattcggga gagcatcact ctctcccgct
     4621 ctgtcagtat tctgaattga cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg
     4681 tttgtaggta gagttggagg cgcgcagaat gcggatacca cgagcgccga catcgcgtgc
     4741 ggcggtaata tcgttatcgg aatcgccata aaaaattcgg atatttttat cctgcagcca
     4801 ttgcgatttt gtattttgcc ctggtttatc gcccgcaaag attaccggat tcatgttggt
     4861 ggcaggaata tgaaaattat ccgccagcgt ttttgaaacc gtttctgttt tcgtcgggct
     4921 acgaccagtc acaaagaaga tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg
     4981 agcgacctct tttggaatgc tgaattcatc ccagccatta ttcatttttt cccagaacac
     5041 aggatttttc agataatctt cgctttctgg cgagaaggtt tttttgccgc gccagaaccc
     5101 cggactggaa aaaagtaccg tgtcatcgat atcaaacccc accgccattg gcggacgccc
     5161 tgcgaggcta ttttcaattt gtgcgaccga aacccaatga attggtgcct gttcagcaag
     5221 cctggcaacg ttagtcccag ggttaagcgg tgaaggagat gaggccaggg caacagcgga
     5281 actgtttaga gcgaacaata agcaaacggc actgattgcc tgtgtgatct tgcgcatatt
     5341 tttccctaaa tagtcagtat gttgaaactt tttattgtga gatttgttgc aaaaaccatc
     5401 tgaccataac gccagctgcc ggggaaagga aggattatct gcggtttttg tgatgaaaag
     5461 caggatgatt atatttaacc cagtaaatac gttcctgtct ttttttgtat aaaaaatgtt
     5521 tattactcga tgtaaggata cattaatttt acgtttatta tgatgaatcc tcggcaaatg
     5581 agcgaaattt gatagaacat ttatctgctg tacgatttaa tgaacttaat gatacagcat
     5641 tacaaaaaca atcgaagttt ataaagatga tttctgattg accagccccc taccctacaa
     5701 tggtgattaa ttttattact cgcgatggag atgatgatat gaatatatca tatgtgaata
     5761 gcaacaaaac gacatcttta cctgttgagc tagatgcact aaataataaa gatatctctt
     5821 atgccaaaga tttcttttta tacatagaaa cacagttaaa aattgcaaaa gatttttgta
     5881 gacctggaga agaagtatca agttctattg caagtaaagt ttttcacgca tttattgatt
     5941 tagttaacaa aatcaggggt aagaaagact ttatgtatat ttgcacgctt tgctgttttg
     6001 ccgaggaggt taaaggtgat tattctcatt acaggacctt tttatttgat attggtaatc
     6061 aatataaggt taaacttaca caaagcggaa aaaagagttc tctttaacat tagaatttaa
     6121 cgatactata attgaatctc agaaagtcac aggcaataag gcaaagcata ttcttgagga
     6181 tatagaaata ttctatcgta acaaacccga tacttattat taaatttaat aatggttaga
     6241 tgtgcagaaa ttattactcc acacgaaaaa atagggaaag gaattccaga taattgggat
     6301 aaacttaaca ataaaagaac tgacaacgtt ttattatctc tcacgtagaa caacggcatc
     6361 aaaaaaatca tctacaaaga aatagtgaaa gcgacgacag tttaatttca ctgcaggctg
     6421 ggtaactcca gcctgctttc ctgcattaca tcaccgcagc aaacgccttt gccacacgtt
     6481 gtacatttgc cgtatttaac ccggcgacac acatgcgacc gctggcgatg agatagacac
     6541 caaattcttc acgtagtcgg tcaacctgag cggcacttaa accggtataa ctgaacatgc
     6601 cgcgctgatt aagcagataa tcgaaattgc gttctggcat ctctgtgctt aataccttca
     6661 ccaattcctg acgcattgcc agaatgcgag tacgcatctc ttctacttcc gccagccagc
     6721 tggctttcaa tgcctcgtca ttcagcactg cagccaccac ctgcgcacca aaattcggcg
     6781 ggctggagta gttgcggcga actgttgctt tcaattgccc cagtacgcgg ccagcggctt
     6841 cggcatcttc acacagaaca gaaagtccgc cgacgcgctc gccgtaaagg gagaaaattt
     6901 tcgagaacga attgctcacc agagcgggta atccagcact ggcaatggcg cgaatagcgt
     6961 aggcatcctc ttccataccg gcactaaatc cttgataggc aatatcgagg aatgggataa
     7021 gctcgcgggc tttgagaatt tcaatcaccg catcccactg atcattagtg agatcggcac
     7081 ccgttgggtt gtggcaacat ggatgcagca acacaatact gcgggcaggt aatgttttca
     7141 gcgtcgccaa caggtcatta aagcgcacgc tgttagtcac ttcgtcatac caggggtaag
     7201 tactcacttc gaatccagcc ccggcgaata ttgctacgtg gttttcccaa gtaggatcgc
     7261 tgacccagac gcctgattcc gggaagtagc gtttcaggaa atccgcgccc actttcaatg
     7321 cccctgagcc gccaagggtt tgaatggttg ctacgcgctg ttgttgcagt accggatggt
     7381 ccgcaccaaa cagcagcggc gcaatggcat ggcgatagct gttaagccct tccatcggta
     7441 aataaagcga agcgccatgg ggctgcgcat tcaggcgcgc ttccgcctcc gccacggctt
     7501 tcaattgtgg aataattccg tcttcgttgt agtacagacc gatacttaaa ttcactttgt
     7561 cgctgcgagg gtcttcttta aaacgctcca taagcgtaag aatcgggtcg ccagcgtagg
     7621 cgtcaacttt ttgaaacacg cgatggttct ccaggtttac gggcaggtgg ttaaaacaca
     7681 ataaaccgga agaaggcgaa gatcgagtgg atgttcagga gcgaacggca attagcaaca
     7741 gagtgagact aatgataaac gtacatccgc cagaggcacg acctttataa gagcatgaaa
     7801 gaacatcaac ttattgaatt tttaggattt tattggccgg ataaggcatt cacgccgcat
     7861 ccggcacaga caatcaaata ttacagaacg attaatccac gtatttcatc gcgacccttg
     7921 aagtcaggcg cgtaataagt tcgtaagcgc ttactttcgt catttcagcg atacgttcta
     7981 cgggcaaacc ttcgccccat aaaatgaccg gatccccggc tttgtcctgc gcctgtggac
     8041 ctaagtctac gcagatcata tccatcgcca cgcgcccgac aatcggtact tcgcgaccgt
     8101 tcaccagcac tggcgtaccg gacggcgcgg cgcgcggata accatcgcca tagcccatcg
     8161 cgactacgcc aagacgggta tcacgttcgc ttacccaggt tccaccataa ccaacaggct
     8221 ctccggcttt atgctcacgc acggcaatca ggctggaggt tagtgacatc actggctgac
     8281 agccaaaatc ggcaccggtg gagcgatctt ccagcggcga gacgccataa agaatgatgc
     8341 ccgggcgcac ccagtcaaaa tgcgactgtg gccacagcag aatgccaccc gacgcggcaa
     8401 tggaacgttg accaggtttg ccttcgcaaa aggtattaaa gatagcgagt tgtttctcgg
     8461 ttgcgccaca ttttggttca tccgcgcgcg caaaatggct gacgatattc accggctgac
     8521 gaacgttttt gcactgggtc aggcgatgat aaaacgcctc agcctgttcc ggccttacgc
     8581 ccagacggtg cataccggta tcgagtttca tccagacggt aaccggctcg tccaagctag
     8641 cctcttccag cgcagccagc tgttcttcgt tatgcacggc ggtatgaaaa tgttgcgcag
     8701 aaatcgtcgg cagatctctg gcatcaaaaa agccttcgag taacagtaca ggtttggtga
     8761 ttccccccgc acgcagtcgc agagcttctt cgagacgggc tacgccaaag gcgtcagcat
     8821 cggggagcgt tcgcgcggtc tcaagaagac cgtgaccata agcgttcgct ttcaccaccg
     8881 caaccatttt actggcaggt gccagttcac gaagacgttg caggttgtgt cgcagagcgc
     8941 ggcggttaat cacaacagtt gccgcttgca tttgtgttcc ttgataattg tttgctttaa
     9001 ttacctaatt cataaaataa ttattattcg tcgtcgtact gcggccccgc atagttgtcg
     9061 aagcgcgacc attgaccgtt aaaggtcagg cgtaccgtcc cgattgggcc gttacgttgt
     9121 ttaccgataa taatttccgc gatgcctttt aaatcactgt tttcgtgata cacctcatca
     9181 cgatagataa acatgatcaa gtccgcatcc tgctcgatag agccagattc acgcaggtcg
     9241 gagttgaccg gacgtttgtc ggcacgttgt tccagagaac ggttcaactg ggacagcgcc
     9301 accaccggca cgttcagttc tttcgccagt gctttcagcg agcgggagat ttctgcaatt
     9361 tccagcgtac ggttatcgga aagcgccggt acgcgcatca gttgcaggta gtcgatcatg
     9421 ataagcccga tgccgccgtg ttcacgggca atacggcgtg cgcgggaacg cacttccgtt
     9481 ggcgtcaggc cggaagagtc atcgatatag atattgcgtt tttcgagcaa aatacccatg
     9541 gtgccggaaa tgcgcgccca gtcttcatca tcgagctgcc cggtacggat tttagtctgg
     9601 tcaacgcgcg acagcgacgc cagagaacgc atcatgatct gttctgatgg catctccagc
     9661 gagaagataa gtactggttt atcctgcaac attgccgcgt tttcgacgag gttcatcgca
     9721 aatgttgttt tacccatcga cggacgcgcg gcgacgatga tcaaatccga cggctgcaag
     9781 ccagcggttt ttttgttgag atcgtcataa ccggtgttta ccccggtaac gccatcgtgt
     9841 ggctgctgaa acaactgctc aatacgcgcc acggttgcgt cgagcacatc ggcgatgttc
     9901 ttcggccctt cgtctttgtt tgcacgactt tcggcaattt taaagacgcg ggattcagca
     9961 aggtccagca gatcttcgct ggtacgcccc tgcggatcaa aaccggcttc agcaatctca
    10021 ttcgcaaccg agatcatctc acggacaacg gcacgttcac gcacgatgtc cgcataagca
    10081 ctgatgttcg ccgcacttgg cgtatttttt gacagctctg ccagataagc aaaaccaccg
    10141 acgctatcga gttgcccctg gcgttccaac gattccgcaa gggtaatcag atcgatagga
    10201 ctaccgcttt cctgcaaacg cgccatttca gtaaagatat gacggtgtgg gcgggtgtaa
    10261 aaatcgtctg ctaccacacg ctcggctaca tcatcccagc gttcgttatc cagcattaaa
    10321 ccgcccaaca ccgactgctc cgcttcgatc gagtgcggag gcactttcag cccggcaact
    10381 tgtggatcgc gttcgcgggg ttcagcctgc tgtttgttga agggtttatt tcctgccata
    10441 gtgaatggag ttaccgaaat aaagaatggg tcgaaacttt accatatgaa gcagaccctg
    10501 acgatacgtt ctggaggaca catggcaaca cgaattgaat ttcacaagca cggtggcccg
    10561 gaagtacttc aagccgtaga gttcactcct gccgatccgg cggagaatga aatccaggtc
    10621 gaaaataaag ccatcggcat caattttatc gacacgtata tccgcagcgg cctttacccg
    10681 ccgccatcgc tacccagcgg attaggtacc gaagcggcag gcatcgtgag taaagtcggc
    10741 agtggtgtaa agcatattaa ggctggcgat catgtagtct atgcgcagtc ggcgttaggc
    10801 gcttacagct ctgtgcataa cattaatgcg gataaagcgg cgattctgcc tgcggcaatt
    10861 tcttttgagc aagctgcggc atccttcctg aaaggcttaa cggtttatta tctgctgcgc
    10921 aaaacctatg aaattaaacc cgatgaacag ttcctgttcc acgcagcggc tggcggcatt
    10981 ggcttaattg cctgccagtg ggcaaaagcc ctgggcgcga aactcatcgg caccgtagga
    11041 accgcgcaaa aagcgcagag cgcgctaaaa gccggcgcgt ggcaggttat taactatcgt
    11101 gaagagaatc tggtcgagcg attgaaagag atcaccggcg gtaagaaagt gcgcgtagtg
    11161 tacgattccg tgggcagaga cacctgggaa cggtcgctgg attgcctgca acgccgcggc
    11221 ttaatggtca gttttggcaa ctcatcaggt gcggttaccg gtgtgaactt aggcattctc
    11281 aatcaaaaag gctcgttgta tgtgacacgc ccttccctgc aaggctatat caccacgcgg
    11341 gaggaattaa ccgaggccag taatgaactg ttctctttga ttgccagcgg tgtgattaag
    11401 gtcgatgtcg ccgagcagca gaaatatccg ctgaaggatg cgcagcgtgc gcatgagatt
    11461 ctggaaagcc gggcgacgca aggttccagc ctgctgattc cataaaagaa atagggcttc
    11521 cacctgggaa gccctttctt tttatagttc ggctgtatgt agggtacagc acgatgaatc
    11581 tgttagaggc gcaatagtga cagatttgat tatcaattcc tattttgttc taaggataaa
    11641 accttaggtt gtgatcatcc gcacaatccc ttagtaacgc cagcggtcat aacgctgata
    11701 tttcggcatt tttggtgctt taatcgcctt aataacccac accaccgcaa tcgccagtag
    11761 taaccacggc agcaacttaa tcatcaatgc cagcataccg ccgaggaaca taatggccgt
    11821 cgccacaacc agcgcggcga taatgcccag caacgaaacg ccggtgacca tcagcatgac
    11881 aaaaaagcca atcacaaaaa gtagttccag catgatgctc tcccaaatat gaaatctctt
    11941 gctggcatta caagaatcat gccaaaaata atctattgat ttaacagcaa aacgccccgc
    12001 gacggtgcgc agggcgtggt gaatttgact actttttggt gaaaagttaa cgcttatccg
    12061 ccaccagttt gagcgcgtgt tccagcacgt taatgtcagc acccgcttta tgggcatttt
    12121 cacttaaata acgccgccac tgccgcgcgc caggaatacc ctggaacaaa cccagcatat
    12181 gccggataat atggccgaga tacgtcccct ggctgagttc atgctcaatg tacggataca
    12241 tggcacgcac tactgccacc ggatcggcat cggtatccga ggaaccaaag atctcccggt
    12301 ctaccgccgc cagaataccc ggattctgat acgcctcgcg cccgaccatc acgccatcca
    12361 tatgttgcaa atgcgcttta gcttcttcca gcgacttgat accaccgtta atcgacattg
    12421 tcagatgcgg gaagtcacgc ttcagttgat acacacgcgg ataatcgagc ggcgggattt
    12481 cacggttttc tttcgggctt aaccccgaaa gccaggcttt acgtgcgtgg atgatgaaca
    12541 cctcacactc gcctttgccg gaaacggtat tgatgaaatc gcagagaaat tcatagctgt
    12601 cctggtcgtc gatgccaata cgcgttttca ccgtcaccgg aatcgacacc acatcgcgca
    12661 tcgctttcac gcagtcggca accagctgcg cattacccat cagacacgca ccaaacatgc
    12721 cgttctgcac ccggtcagac gggcagccga cattcaggtt gatctcatca tatccgcgcg
    12781 cttctgccag ctttgcacac tgcgccagcg ccgccggatc gctaccgccc agttgcaacg
    12841 ctaccggatg ttcttcttca ctgtacgcca gataatcacc tttaccgtga attatcgccc
    12901 ctgtggtcac catttcggta taaagcaacg tattgcggga aagcagacgc aagaaatagc
    12961 ggcaatgtct gtccgtccag tcgagcattg gtgcaacact aaaccgacca ctccagtgaa
    13021 cgtcagtttt ttcaggcatt gcgctggttt ggttgatttt ttgcatttca gaattaccgt
    13081 gcattttcaa atgtagagat tattttatcg atatatcatg gggttatgtt attcagcttc
    13141 actgttcagg aggctcaata gcggggtact ataccataac aacaggaagc gcctgtctca
    13201 ttgcaaaaga aaattgagat cctctcaagg catgaagctc tcacgaagtg atggaaataa
    13261 tcttattagc cgttaacttt gttaaggcca atgataaaca atccaggttc gacgataaat
    13321 aaaaaatcac acattaaact ctggtgatat atctccctgc taatagcatt gatagagaaa
    13381 aaagaaccca ataaatattg ggtcctttta tataatgcct tccatactat cgaagaactt
    13441 cacatattat ttctccgatt taaccccgaa caagtcataa attaatttag aagtgtctgt
    13501 aagtatttta atctcttcct ttgaggttgg gtcaaacgac ttcgcgaagc taattaattg
    13561 tggtgcagca tctcgcattt tgcttaaaat atcaggatcg agcgttcctt cattcaccag
    13621 atgtgacatc ttatccagat agccatcaaa attcatttct cccccatctg ccagacgctt
    13681 catctctccc aggtacttct tcatatcacg ttgatataat ttttcaaact gagctttcag
    13741 gtagctctca ttattttcat taatatacac tgtttctgat aaatctgcgt aggcactaga
    13801 atatgaaagt gaaattaaca gcgaagcaaa taattttaag ctgttgtcat catgttcctg
    13861 gcaggcatta acaaacgtta aaaaaccaga acctattgct tgaaaatgta tattcgctaa
    13921 aggttcatta tccttagatt cctcataaaa tagctgaacc gtggagggaa ggccattctt
    13981 agtttctaca tcaaaagtac attgttcaat gggcattgca tctttattat ccccaggatt
    14041 acatatacta ttaatcgcga aatgaaaaat agcccggtca acaactgatg caataaagtc
    14101 acgatcgttt aaatcctcat cagtgcaatc gtcagtatac ttattgaccc acattttgag
    14161 atcatttatg tgctcaatta aaaacacatt tttttacgtg catgccataa attatgcgtg
    14221 attcgttgaa taaacaaatc tatttttgca ataattatat cccgatgacg cagcgctgca
    14281 taatcaatag atttaccata tatattattt aatttacctt tgattgaacc tctattatcc
    14341 aatttatctt tggttgcatc tttattttcg aattgatttg aattgagtgt tttattaaag
    14401 tatccagttg caccgggtat tatcttcaac atgattcatc tacctttttt gtcgtaccgt
    14461 gtcctcctta taattctttt gccatctggt cactatcaaa aatcgctcat aatttctgaa
    14521 ctacaggtat gcgtttaaac ccagttaaat aaatagcgag ttaacttgaa gcaatttatt
    14581 taaaaatgaa ttattaatta ttatcgtgtt gataatggta cgataaggca acacaacacc
    14641 ttacctacaa aacagaaaat caacattacg gcaacaataa gggttctcgg tgtttttcct
    14701 ggaacatggt aaagtaagga cattcttaac ccccactttg aggtgcccga tggaaaagac
    14761 cacaacgcag gagttattag cgcaggctga aaaaatctgc gcgcagcgta atgtgcgcct
    14821 gaccccacag cgcctggaag tgttgcgcct gatgagtctg caggatggcg ctatcagcgc
    14881 ttatgatctg cttgatttac tgcgcgaagc tgaaccgcaa gccaagccgc caacggttta
    14941 tcgcgcgctg gattttctgc ttgagcaagg ttttgtgcat aaggtggaat ccaccaacag
    15001 ttatgtgctc tgtcatctgt tcgaccagcc cacgcatacg tcagccatgt ttatttgcga
    15061 tcgctgcggc gcggtgaaag aagagtgtgc agaaggcgtg gaagacatta tgcatacgct
    15121 ggcggcaaaa atggggtttg ccctgcggca taatgtgatt gaagcacatg ggctctgtgc
    15181 ggcatgcgtg gaagtggaag cgtgtcgtca tcctgaacag tgccagcatg atcactctgt
    15241 gcaggtgaaa aagaaaccgc gttaagaggg cgtacatcct tgtacacgtc gggcaggagg
    15301 gattaattac cagcgatatt cattgcgggt ttcccaatcc acgacctctt tttctgcctg
    15361 atctttctga taaccataac gttcctggat tttaccgacc agttgatcac gtttaccttc
    15421 aatgatcgtc atatcatcat cggtcagttt gccccattgc tctttcactt tacctttaaa
    15481 ctgtttccag ttaccgccgg cttcatcttt attcataatc aagacctcat cgttaggttg
    15541 tgaatgagag tacgttcact tttcttctga acgtgagatt aagtgtagtc agcaatttgg
    15601 cttaggataa tttattcaga atttttaacc gtcacgttgc ggcaaaccag gtaccgttgc
    15661 gccagtgacg ccgccagata gccgccagag aaagcccgcg caacgccaga aagacggtta
    15721 atgccagcca caaaccatga ttacccagcc acggcagcgt aaggagcgtc agcgcaaaac
    15781 ctgcggcggc caccgccata ctgttacgca tttcggcggc acgcgttgcg cctataaaca
    15841 tgccgtccag cagataacac cagacgccaa ccaacggcaa aatcacctgc cagataagat
    15901 agcggtcagc cagctgctga atctgggtta acgacgtcag caacgcaatg atgtgttccc
    15961 ctgccagcaa ataaaccacc gaaaatagta acgctacaat ccccgactgg cggcacgctg
    16021 cccgccatac atccagcaac tggctaccgt cgcgcgcacc gtatgcctga ccggagtgcg
    16081 cttcaaccgc gtaggcaaaa ccatcaagcg cataggcggt aaaggtgagt agcgtcatca
    16141 gaaccgcgtt aacagcgata atgtcactcc ccagtcgcgc accaagtacg gtgatcgcgc
    16201 cgaaacagag ttgcaacaac agcgaacgca gcatgatatc gcggttaagc gccagcaagc
    16261 gacggaagtt tcctcgccag gcagttttca gcatttcgcc ggaaattccg cgtagtttga
    16321 ggattttacg caccattagc agaccaatca gcaatgttgc atattccgca ataaccgtcg
    16381 ccagcgccgc gccctgcacg ttcatatgca gccccatcac cagccagaca tccagcacaa
    16441 tgttgaggat attgccgacc actaacaaaa ttactggcgc acgggcatat tgcacgccga
    16501 gtaaccaacc gagtaatacc agattcgcca gcgacgccgg agcgcttaac cagcggattt
    16561 caagaaagcg ccgcgcctgt tccaggaccg cttcactacc gccaacaata tgcagcgcca
    16621 gatcgataat cggcgtacgc aacagcgcaa ttaacgcccc ggcccccaac gccaacagca
    16681 acggttgcac cagcgcacgg gctaatgcct gaggattttt ggcaccataa gcctgcgcag
    16741 tcagcccggt ggtgctcatg cgtaaaaaca gcaacagcat aaagagaaag ctggtcgccg
    16801 ttgcaccaac cgccacgccg cccaaataaa ccggactatc aagatgacca attaccgccg
    16861 tatcgaccag tcccagcaac ggaacggtga tattggagaa aatcatgggt aaggcgagat
    16921 gccagagtgc tttatcagat gaagtgagga atgccatgca gacaagcctg atgaagagag
    16981 atgaaaaaca aaccgcgacg ccaagcggca tcgcggtctc agagatatgt tacagccagt
    17041 cgccgttgcg aataacccca accgccagcc cttcaatggt gaagctctgc tgacgaaggt
    17101 caacgacaat tggtttaaac tcgctatttt ctggcaacag ttcgacttta ttgccctgtt
    17161 ttttcaggcg cttaacggta acttcgtcat caatacgtgc gacaacgacc tgaccgttac
    17221 gtacatcctg agttttatgc actgccagca agtcgccatc cataatgccg atatctttca
    17281 tcgacatccc gctgacgcgc agcaggaaat cagcattcgg cttgaacaag gaaggatcga
    17341 cctgataatg accttcaata tgctgttgcg ccagaagcgg ttcaccggca gccacacgac
    17401 ctaccagcgg caacccttct tcctcttcct gcaacagacg aatcccgcgt gatgcgccgg
    17461 aaacaatttc aataacgcct ttgcgtgcca gcgccttcag atgttcttca gccgcgtttg
    17521 gggaacggaa ccccaaacgc tgcgcgattt ccgcacgcgt cggcggcata cctgtctggc
    17581 tgatgtgatc acggatgaga tcaaacacct cttgttgcct ggccgttaac gctttcattc
    17641 cgccccctgg gtgtatatac agttatactg tgagtatata cagcaaaagg cgattttgga
    17701 accataaact gcacaataaa ccagagattt atcgaaatct ggaagggtta tccaaaatgc
    17761 gaccataaca ggatgcacca ggtaatcacg gcgacgataa tggcaatcag caccgcggcg
    17821 gatcccatat cttttgcgcg tccggaaagc tcatggtatt cagagccaat tcggtcaacc
    17881 actgcttcga tggcgctatt gaggatttcc acaatcatca ccagcatcac ggagctgata
    17941 agcagcacgc gggtaattgc gtccacatcc agccagcagg cgatgaccac cgccaacaat
    18001 accgctacgc cttcctgacg gaatgccgct tcgttgatcc atgcagcgcg taaacctttc
    18061 caggaatagc cagcagcttt gataattcgg gtgaatccag ttgtattatt ggccattgaa
    18121 agaacctttt tacattatga gcgtcaatat cagtgtaccg ttaaatccga tgattcgggt
    18181 ttgcgtaacg ccgcgcgaaa catgagcgga taccacagaa tttcccatga ctttctgcta
    18241 tccttgccgc gcatttgcat tattaaccag aggctttaca tcgtttatgt ccggctggcc
    18301 acgaatttac tacaaattac tgaatttacc attaagcatc ctggtaaaaa gcaagtctat
    18361 tccggcagat cctgccccgg aactggggct ggatacctct cgtccaatta tgtacgtttt
    18421 accgtacaac tcgaaagcag atttgctgac gttgcgcgcc cagtgtctgg cacatgattt
    18481 gcctgacccg ttagagccgc tggaaatcga cggcacgcta ctgccgcgct atgtgttcat
    18541 tcacggcggg ccgcgtgtat tcacctatta cacgccgaaa gaagagtcta ttaagctttt
    18601 ccacgactat ctcgatttgc accgtagcaa cccaaatctg gatgtgcaga tggtgccagt
    18661 atcggtgatg tttggtcgcg cgccggggcg tgaaaaaggc gaagtgaacc cgccgctgcg
    18721 tatgcttaac ggcgtacaga aatttttcgc tgtactgtgg ctcggtcgcg acagttttgt
    18781 gcgtttctcg ccgtcagtgt cgctgcgccg tatggcggat gaacacggca cggataaaac
    18841 tatcgctcag aaactggcgc gcgtggcgcg tatgcacttt gcccgtcaac gtctggctgc
    18901 cgtaggccca cgtctgcctg ctcgtcagga tctgtttaat aagctgctcg cctcccgcgc
    18961 cattgccaaa gcggtagaag atgaagcgcg cagcaaaaaa atctcccatg aaaaagcgca
    19021 gcagaacgcg attgcgctga tggaagagat tgcggcgaat ttctcttacg agatgatccg
    19081 cctgactgac cgcattctgg gcttcacctg gaaccgattt tatcagggca tcaacgtcca
    19141 taacgccgag cgcgttcgcc agctggccca cgacggtcat gagctggtgt atgtgccttg
    19201 ccaccgcagt cacatggact acctgctact ttcttacgtg ctgtaccacc agggactggt
    19261 gccgccgcat atcgccgccg ggatcaacct gaacttctgg ccggccgggc cgattttccg
    19321 ccgtctgggg gcgttcttta ttcgccgtac ttttaaaggc aataaacttt attccaccgt
    19381 tttccgtgag tatctcggcg aactgttcag ccgtggttat tccgtcgagt acttcgtgga
    19441 aggcggtcgt tcccgcacgg ggcgtttgct ggatccgaaa accggtacgc tgtcgatgac
    19501 cattcaggcg atgctgcgtg gcggcacgcg tccgattacg ctgattccga tctatatcgg
    19561 ttatgagcac gtcatggaag tgggtactta cgccaaagaa ctgcgcggcg cgacgaaaga
    19621 gaaagagagc ctgccgcaga tgctgcgcgg tttaagcaag ctgcgtaatc tcggtcaggg
    19681 ttacgtcaac ttcggtgaac caatgccgtt gatgacctac cttaaccagc acgtaccaga
    19741 ctggcgtgaa tctatcgatc ccatcgaagc ggtgcgtccg gcctggttaa cgccgacggt
    19801 caataatatt gctgccgatc tgatggtacg cattaacaac gcaggcgcgg caaacgccat
    19861 gaacctgtgc tgtactgcgc tactggcatc acgtcagcgc tcactcaccc gcgagcagtt
    19921 aaccgagcaa ctcaactgct acctggatct gatgcgcaac gtaccttact ccacggactc
    19981 taccgttcct tcagccagcg ccagcgagct tatcgatcac gcgctgcaaa tgaacaagtt
    20041 tgaagtcgag aaagacacta tcggcgacat catcattctg ccacgcgagc aagcggtgct
    20101 gatgacctac tatcgcaaca acattgcgca tatgttggtg ctgccttcgc tgatggcggc
    20161 aattgtcacc cagcatcgcc acatctcccg cgacgtattg atggagcacg tcaatgtgct
    20221 ttacccaatg ctgaaagcgg agctgttcct gcgctgggat cgcgacgagt tgccggacgt
    20281 tattgatgcg ctggcaaatg agatgcaacg tcaggggctg attaccctgc aagatgatga
    20341 gttgcatatc aacccggcgc attctcgcac cctacagctg ctggccgcag gcgcgcgcga
    20401 aacgctgcaa cgttatgcca tcaccttctg gttgttgagt gccaacccgt cgatcaaccg
    20461 cggtacgctg gagaaagaga gccgcaccgt tgcgcaacgt ctctccgtgc tgcacggcat
    20521 caacgcgccg gagttcttcg acaaggcggt gttcagttct ctggtgctga cgctgcgtga
    20581 tgaagggtat atcagcgata gcggcgatgc cgaaccagca gaaacgatga aggtttatca
    20641 gttgctggcg gagttgatta catcagacgt gcgtttgacg attgagagtg cgacgcaggg
    20701 cgaagggtaa tcagagagaa ttgccggatg cggcgaaaac gccttatccg gcctaccatg
    20761 acctgcaaat tcaataaatt gcgattcacc gggtaggcct gagaagcgca acgcatcagg
    20821 caacccagaa gaaagccgga tgatcatccg gcttttttac atcatcagaa atgccagtaa
    20881 ctcattgcca gtcctaaaaa cagtaccaga ccaacatagt tattattcat aaatgcttta
    20941 aagcaggctt cacgctcgcg gttggcaatc agtttttgtt gataaacaaa cagcgcgcca
    21001 gccaccacaa ttgaccagta atatccccag cctaagccat ttaactcacc gatgatcgcc
    21061 atcagtgcca gtacgccaat ctgcaaaata ccaataatca atttatcgta ttggccgaac
    21121 aggattgccg tggatttaat gccaatcttc acatcatcat cgcggtcaac catcgcatac
    21181 tgcgtgtcgt aagccaccgc ccagagaata ttggcgagga acattaacca gcaactcaat
    21241 ggcaccgact cactcacagc ggcaaaagcc attggaatcg accagccaaa cgccgcgccc
    21301 agcaccactt gcgggagatg ggtataccgc ttcataaacg ggtacaccca cgccaatgcc
    21361 agagcggcaa tcgacaacag aatggtcatc gtattcagcg tcagaaccag taaaaacgaa
    21421 atcagtacca gcactacaaa cagcgcgcgc gcctctttct ctgttaccgc gccgctggga
    21481 agtggtcggt tcgccgtgcg cttaacatga ccatcaaact tgcgatcagc ataatcattc
    21541 accacgcaac cagcagcgcg catcagccag acacccgcga caaacaccgc catgatccag
    21601 agctgaggaa cgcccggtgt cgccacccac aacgcccata atgttggcca gagcagcagt
    21661 aacgcgccaa ttggcttatc cgtacgcatt aagcgatgaa acgccagcag cttattctgc
    21721 gtcagactcc actccatatt tttttcctct tagtacaacg gtgacgccgg taaaaacagt
    21781 tctgttagca acagcggttt accgcttaat cgcaggcggg aacgtcgccc ccacagtccg
    21841 gcatcacggc ctatctcgat aaaatcccgg gttagtgtcg atgatgtgaa cagatagcgt
    21901 cctaacggcg ttttacccaa tttttgtaac gctagttccg gcccgcttaa tgttgataca
    21961 ggaacgacgg tacgaccggc aagccacggt tcaccatcgg cacataacaa aatttcacgt
    22021 aaccagtaac gagactcttt cggcagcagc ggcagttctt cggggatttc attctgctcg
    22081 acaaacccct cgcggatcat cgtcacactc accgtttttc cctgctgttc aaaacgtttt
    22141 gtcatggaat cctccagcaa cagccagtcg agcagttgcg gatccagggc agggatctct
    22201 ttaaaatagc gcagcgcacg cagttgcgtt aacgcggggt gtgacatgcc gaactctccg
    22261 ttacataacg taaaggcatt gtatcgcaga aaagtgttta cgggagatgt aaagcaaatt
    22321 aagtgatcta atgcgcaaca aaaacgcaac aatcgcatga agtggtaaaa cgctgttgaa
    22381 ccttacgctt tgccgatgcc cgtgctggcg agggcaacca taggggcatc accgcccatg
    22441 atgctaggaa cggtgatgtt ttcctttact taaatacctt tattctttat acgagtagct
    22501 cgccaactcg cagtaaaaat tgggaaaagc gcatatttac catgagattt ggagctgaaa
    22561 agtgtttcct gagtaagatt aataggcaac atcttatgtc tggatttata gatacacgtg
    22621 gaattccaat tggtatcttt tgtaggattc aacatatcat gtaagcgctg ggagcttacc
    22681 accataacag gcccatcatc gacagaggag aacaggataa atatattatc gtttcgtgtt
    22741 aatttattag tgttaaaggt tcttaacttt tcttcagttt ctataaatat aggtgaaaca
    22801 tcatcactac gaatccactc tttataaaga taccaggcat ttgctttgat catatcatcc
    22861 gttgatcgta aggcctgatc gataagttgg ataaaaaaat tgttattttt atcaaccagc
    22921 cctttattca tattaaatct gttaataaaa tggatcatct gttgggtatc gaaattcacc
    22981 ttactgaatc gatgttgacg cataaaatta tcgaacttgt catcttcccc agaatgtata
    23041 taactacgta taattggatc ttttatataa ggacaatggc taaatgtttt cttcatattc
    23101 tcaataaagt ttttattaac aatattgaga atttttaatt gcatcaccag ttttactgcc
    23161 agaacggatt tcaatatcat tgcattatct ggcaatgtat caatcatttt tataatgtta
    23221 ttgatataac catcgagatc ttcattccca aatatatctg aataatcaat agttaattcc
    23281 aattgttctg taatgcaatc ttaaaaattc cctgtcgaca ttcgcacatg atcaaacata
    23341 acagctgtca aattcgattt aataaatgat atttctttgg cagacagaaa ctctgctctg
    23401 gtgaagtgcg aagcgtaaag ataacaatta tcgaagtttg aatttctaat ctgacattcg
    23461 cgaaaattac tatgaccaag ttgactctgc gagaagtcaa ctgtatcaag aatattttgg
    23521 ctaaaatcaa cacattcgcc actcgttttg agaaaaatca cattgtgtaa acgtcgacat
    23581 ttaaatatga cattatcaac tgttgcctta gtaaaattgc aattctcaat ttctttacat
    23641 tcgaaagtta catgttgtag atttgatgat aaaaaagaca ccttagaaat aacgtcacct
    23701 ttaaaatgac aatttgttaa atcaaaatca gacaggtcga tatacccctg cgcatcttta
    23761 tccatcagaa aattacaagc gcattctatt ttttcatttc tgtagaattt tacacttata
    23821 gtatcattac taattgagac ttcacgtaac atatttattg catgattttt ttcgttccgt
    23881 tgttttatat ttaaaaatat atcactgata taaataaagt aatctctgtt taaacctgtc
    23941 accccattga aattacgtag taaatcaaga tctactatat tatttccttg caccacatca
    24001 tgtttcatca aagagttatt gccgccagaa tttgtgatat gacctatcat aaaacctctg
    24061 taatacagaa ataaaataac ctggaaaagt agcctgagcg tccagacact accggcgaaa
    24121 ggaaaagaat ctctgataat gcattgaaat aatggacatt cttagtgaag aagaagtgaa
    24181 gtactataaa aaatcgctca tatcccagga aggaagcata aaaaaaggtg cgccaggaga
    24241 cgcaccagtt gttgcaaaat cagcactgtg gggcgtaatt accccttgcc ttttacactg
    24301 ctgataaagg tggaacgggc agatgtcgat cccaggcgtt cagcttcatc aagcagtttt
    24361 aacgccttat caacatcacc tttcgcgaca gcgtttttga tcgcggtatt aaaataactt
    24421 tccgtgtcgt tgagcatcgg ctcgcttttc ttcaccggca ccggagcagg tgcagccaca
    24481 gctggtgccg ccgtgttgcc taccgtaacc ggagctggag cggaagaacc aaatagcggt
    24541 cctaccaaca cgctggagct ggagttcgtt ttgactttca gtttcagtaa gccatcgctg
    24601 gtatgacgag caaccggatc gggaatatcc gggatcgagt taccgacgcc tttggcatag
    24661 gctttagccg ggtcgagcag ttgggtcgtc tgctggagat ctttttccgt ggtaaagacc
    24721 agaacataaa gtttttgctg ccccaacgcc ggtgtcaggc gcataacgcc ttccagccga
    24781 tctgcactca tcacgcctag ttcctggtag gtgaaataac tgctggggaa gaaggctgat
    24841 ggggtcatgt tctgatcaag aatcagcacg ttcggcgcaa aaacgctggt ttgtttgttc
    24901 acttcgctgg tcagcgtcag ggtcagttcg ccaatgtttg ccgggacgct gtacgcagca
    24961 accggaccac tgatgccggg aacgttcagt tgttggccgc cggtcgccag ttgggtggtc
    25021 tgggttttag attgatcgac cggtgtccag gtgagttgtt gcagcgcggc agatggaatg
    25081 gctggcgcgt cgctggtgtt ttgcggtacg tagttaacat cggcaaggct aattccaggc
    25141 gcgcttgcca gtaaccctgc tgataaacag agggcgatga gacttttatt cattttcatt
    25201 gttttcacct caaaatctgg agctcagcgg tagccaggca atagcgcgct aaacccgata
    25261 atcagagggg ctttcgcccc tttcagataa tgacaacctg tttttatgcc ggatgcggcg
    25321 taaacgcctt atccggccca ggttttgcta ttaccaccag atttccatct gggcaccgaa
    25381 ggtccactcg tcgctgtcgc cacgaccgaa gctgccgccg ttgaaatcag caggaacggc
    25441 tttgccgtag ttcgggttaa ccttgctatc gccgttgtag tcgtaacccc atttctcatc
    25501 ccacttggcg taggttgcga agacacgaat agccgggcgt gaccagatgc tgtcgccagc
    25561 ctgccattgt tgtgcgaggg taattttgta ctgattgttc ttgtcgccgg tgcgctggga
    25621 ttcgacgttg tcgtagccga tttccatcac ggtgctcatg attggcgtcc acttgtacat
    25681 cgggcgaata ccaacggtcc accacttggt gccgttgtcg ttatcccagt tgatatcctg
    25741 gtacataccc acgtacatca tgtcccagtt gtcgcccatg gagatcgcac cgtggtcgag
    25801 gatacgcagc atgtgaccgt tgttgttgat attgtaggca aatttttcgt tatcaaacgc
    25861 gacgccagaa ccctgcgaca gacctttacc ctgcgaggtc atcgagtcag tagcgtactg
    25921 aacaacaaac ttgttaaagc ccttcaggac actctgagta tgttcagcag tgaataacca
    25981 gccgtctttc gatgcgccat caaccagacg atagttatca cgcaggttgg cacgaccgta
    26041 gtcgacaccc agttctaatg tgccgcccgg gttgatttcc atctgcgcta aacgcacatc
    26101 gaaaacgtcg ttcgcggttt cgttggtata gtcataaata ttgttgctgg caaaagagga
    26161 agaaccacca gcttcagagg agcgggttgc tgccagagag agtttaccga agccaacatc
    26221 gatgttttcc agaccggcac caggaccaga aatatcccag tagtagaagt cgatcatatg
    26281 aacgtcatga cgttggtaga agcgcttacc tgcccagatg gtggaacctg gcagccattc
    26341 gatcaggttt tttccctgca cgtttgcttc acggaaggcc ggatcggtag cttcccagtc
    26401 attctgttgc gcgacggaat aggccacgtt agtgtcgaaa tagaagctct tatcgccctc
    26461 tttccacact tcctgaccca attttaattc agcataagtt tcacattcgt tgccaagacg
    26521 gtatttactt tgagcaccgg tagtctggaa acactgttgt tcaccgccgc tacctgtcca
    26581 gccaataccg gaacgtgcat agccgtggaa atcaacagcc attgcctgag cagacattac
    26641 gcccgctgcg acggcaaccg ccagaggaag tttgcgcaga gtaatcatca ttctatctcc
    26701 tgagtcattg cttttctttt ttcacatcac ctgtgacagg ctttgtgtgt tttgtggggt
    26761 gcttaaacgc ccggctcctt atgcagtcga cgacatgcag tgccatcctc acggaacaga
    26821 tggcaacgct ctggcggcag gccgatagcg aatgtggcac cttcttctac caacaccacg
    26881 tcgttctggc ggtacaccag gttttgacga atggaaggga tctggatata gatttgggtt
    26941 tcgttgccga gttgctcgac gacctgaact tcaccctcaa ggatgacgtc agcgatatca
    27001 ctcggcagta gatgttccgg gcgaataccc agcgacatat tggctccaac ctggacatca
    27061 cggctttcaa caggcagcca gacttgttga cgatttggca tcggcagctc cacctgcact
    27121 tgatcgattg cggtggcggt cacttttacc ggcaggaagt tcatctttgg cgaaccgata
    27181 aatccggcga caaaacggtc tgccggatag tggtacagtt ccagcggttt cccaacctgt
    27241 gccacgcgac cggcgtccag caccacgatt ttgtcggcca gcgtcatcgc ttcgacctga
    27301 tcgtgggtga cgtaaatcat tgtgcggccc aggcgtttat gcagacggga gatttcgata
    27361 cgcatttgca cacgcagtgc agcatcgagg ttggagagcg gttcatcgag caaaaatacg
    27421 cttggctcgg ccaccagcgt acggccaatc gccacgcgct gacgctgacc accggagagc
    27481 gctttcggtt tgcgatccag caaatgcgcc agttgcagca cttccgccac ctggttgacg
    27541 cgttggttaa tcacctcttt ttttgcgcca gccagtttca ggccaaatga catgttttct
    27601 gctactgaca ggtggggata gagcgcgtaa gactgaaaca ccataccaac gccgcgttct
    27661 gctggcggag tgtcattcat ccgtttctca ccgatgaaca ggtcgccgct ggtgatcgtc
    27721 tcaagcccgg caatcatgcg cagtaaagtc gatttaccgc agccagacgg tccgacaaac
    27781 accacgaatt caccttcatg gatatcgaga ttgatatctt tcgataccac gacctcgccc
    27841 caggctttcg ttacattttg cagctgtacg ctcgccatgc ccttctccct ttgtaacaac
    27901 ctgtcatcga cagcaacatt catgatgggc tgactatgcg tcatcaggag atggcttaaa
    27961 tcctccaccc cctggctttt ttatggggga ggaggcggga ggatgagaac gcggcttctg
    28021 tgaactaaac cgaggtcatg taaggaattt cgtgatgttg cttgcaaaaa tcgtggcgat
    28081 tttatgtgcg catccccaca ttaccgccaa ttctgtaaca gagatcacac aaagcaacgg
    28141 tggggcgtag gggcaaggag gatggaaaga ggttgccgta taaagaaact agagtccgtt
    28201 taggtgtttt cacgagcact tcaccaacaa ggaccataga ttatgaaaat aaaaacaggt
    28261 gcacgcatcc tcgcattatc cgcattaacg acgatgatgt tttccgcctc ggctctcgcc
    28321 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
    28381 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
    28441 gataaactgg aagagaaatt ccctcaggtt gcggcaactg gcgatggccc tgacattatc
    28501 ttctgggcgc acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
    28561 ccggacaaag cgttccagga caagctgtat ccgtttacat gggatgccgt acgttacaac
    28621 ggcaagctga ttgcttaccc aatcgctgtt gaagcgttat cgctgattta taacaaagac
    28681 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
    28741 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
    28801 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
    28861 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
    28921 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc
    28981 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg
    29041 aattatggtg taacggtact gccgaccttc aagggtcaac catctaaacc gttcgttggc
    29101 gtgctgagca ccggtattaa cgccgccagt ccgaacaaag agctggcgaa agagttcctc
    29161 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt
    29221 gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccacc
    29281 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg
    29341 tatgccgtac gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc
    29401 ctgaaagacg cgcagactcg tatcaccaag taatgctgtg aaatgccgga tgcggcgtga
    29461 acgccttgtc cggcctacaa aactgaaacg catgtaggcc tgataagacg catcagtgtc
    29521 gcatcaggca gttgttgtcg gataaggcgt gaaagcctta tccgtcctgg aatgaggaag
    29581 aaccccatgg atgtcattaa aaagaaacat tggtggcaaa gcgacgcgct gaaatggtca
    29641 gtgctaggtc tgctcggcct gctggtgggt taccttgttg ttttaatgta cgcacaaggg
    29701 gaatacctgt tcgccattac cacgctgata ttgagttcag cggggctgta tattttcgcc
    29761 aatcgtaaag cctacgcctg gcgctatgtt tacccgggaa tggctggaat gggattattc
    29821 gtcctcttcc ctctggtctg caccatcgcc attgccttca ccaactacag cagcactaac
    29881 cagctgactt ttgaacgtgc gcaggaagtg ttgttagatc gctcctggca agcaggcaaa
    29941 acctataact ttggtcttta cccggcgggc gatgagtggc aactggcgct cagcgacggc
    30001 gaaaccggca aaaattacct ctccgacgcg tttaaatttg gcggcgagca aaaactgcaa
    30061 ctgaaagaaa cgaccgccca gcccgaaggc gaacgcgcga atctgcgcgt gattacccag
    30121 aatcgtcagg cgctgagtga cattaccgcc attctgccgg atggcaacaa agtgatgatg
    30181 agctccctgc gccagttttc tggcacgcag ccgctctaca cactcgacgg tgacggcacg
    30241 ttgacgaata atcagagtgg cgtgaaatat cgtccgaata accaaattgg cttttaccag
    30301 tccattaccg ccgacggcaa ctggggggat gaaaagctaa gccccggtta caccgtgacc
    30361 accggctgga aaaactttac ccgcgtcttt accgacgaag gcattcagaa accgttcctc
    30421 gccattttcg tctggaccgt ggtgttctcg ctgatcactg tctttttaac ggtggcggtc
    30481 ggcatggttc tggcatgtct ggtgcagtgg gaagcgttgc gcggcaaagc ggtctatcgc
    30541 gtcctgctga ttctgcccta cgcggtgcca tcgttcattt caatcttgat tttcaaagga
    30601 ttgtttaacc agagcttcgg tgaaatcaac atgatgttga gcgcgctgtt tggcgtgaag
    30661 cccgcctggt tcagcgatcc gaccaccgct cgcacgatgc tgattatcgt caatacctgg
    30721 ctgggttatc cgtacatgat gatcctctgc atgggcttgc tgaaagcgat tccggacgat
    30781 ttgtatgaag cctcagcaat ggatggcgca ggtccgttcc agaacttctt taagattacg
    30841 ctgccgctgc tgattaaacc gctgacgccg ctgatgatcg ccagcttcgc ctttaacttt
    30901 aacaacttcg tgctgattca gctgttaacc aacggcggcc cggatcgtct tggcacgacc
    30961 acgccagccg gttataccga cctgcttgtt aactacacct accgtatcgc ttttgaaggc
    31021 ggcgggggtc aggacttcgg tctggcggca gcaattgcca cgctgatctt cctgctggtg
    31081 ggtgcgctgg cgatagtgaa cctgaaagcc acgcgaatga agtttgatta agggagataa
    31141 caaaaatggc aatggtccaa ccgaaatcgc aaaaagcacg tttgtttatt actcacctgc
    31201 tactgctact ttttatcgcg gcgattatgt tcccgctgct gatggtcgtc gctatctcgc
    31261 tgcgtcaggg aaacttcgcg accggtagcc tgatcccgga gcaaatctcc tgggatcact
    31321 ggaaactggc gttaggtttt agcgttgaac aggctgatgg tcgcattacg ccaccgccat
    31381 tcccggtact gctgtggctg tggaactcgg taaaggtcgc tgggatttcc gcgattggca
    31441 ttgtggcgct ctccaccacc tgcgcctacg ctttcgcccg tatgcgcttt ccaggcaaag
    31501 cgacgctgct gaaaggaatg ctgattttcc agatgttccc ggcagtactt tcactggtcg
    31561 cgttgtatgc gttgtttgat cgtctgggtg agtacattcc attcattggc ctgaatactc
    31621 acggcggcgt aattttcgcg tatctgggtg ggattgcgct gcatgtctgg accatcaaag
    31681 gctatttcga aaccatcgac agttcgctgg aagaagcggc tgcgctggat ggtgcgacac
    31741 cgtggcaggc cttccgcctt gtcctgttgc cgctgtcagt accgattctg gcggtggtat
    31801 tcatcctgtc gtttatcgct gccattactg aagttccggt cgcgtcgctg ttactgcgtg
    31861 acgtaaacag ctacaccctg gccgtgggga tgcagcaata cctcaacccg caaaactacc
    31921 tgtggggtga ctttgccgcc gctgccgtga tgtctgcatt accgatcacc atcgtcttct
    31981 tgctggctca acgctggctg gtcaacggcc tgacggcagg tggtgtgaaa ggttaaagat
    32041 gttgttctgc caatgttatg ccgctgcacc ctcaacttac gttatcccaa cttgtgacta
    32101 ttattcggcg ctccacggag cgcctttttt tctttcgtct gcaatctgaa tcgttcgccg
    32161 gttaatattc ccatcataga acttattatt tttacgttat ttgttttccc acttacgata
    32221 attctctttc gtgctctgag tcacggcaat agtattgttt catcaatttt tgataattat
    32281 cacaattaag atcacagaaa agacattacg taaacgcatt gtaaaaaatg ataattgcct
    32341 taactgcctg acaattccaa catcaatgca ctgataaaag atcagaatgg tctaaggcag
    32401 gtctgaatga atacccagta taattccagt tatatatttt cgattacctt agtcgctaca
    32461 ttaggtggtt tattatttgg ctacgacacc gccgttattt ccggtactgt tgagtcactc
    32521 aataccgtct ttgttgctcc acaaaactta agtgaatccg ctgccaactc cctgttagga
    32581 ttttgcgtag ccagcgctct gattggttgc atcatcggcg gtgccctcgg tggttattgc
    32641 agtaaccgct tcggccgtcg tgattcactt aagatttctg ctgtcctgtt ttttatttct
    32701 ggtgtaggtt ctgcctggcc agaacttggt tttacctcta taaacccgga caacacagta
    32761 cctatttatc tggcaggtta tgtcccggaa tttgttattt atcgtattat tggcggtatt
    32821 ggcgttggtt tagcctcaat gctttcacca atgtatattg cggaactggc tccagctcat
    32881 attcgcggaa aactggtttc atttaaccag tttgcgatta ttttcgggca acttttagtt
    32941 tactgcgtaa actattttat tgcccgttcc ggtgatgcca gctggctgaa tactgacggc
    33001 aggcgttata tgtttgcctc ggaatgtatc cctgcactgc tgttcttaat gctgctgtat
    33061 accgtgccag aaagtcctcg ctggctgatg tcacgcggca agcaagaaca gacggaaagt
    33121 atcctgcgca aaattatggg caacacgctt gcaactcagg cagtacagga aattaaacac
    33181 tccctggatc atggccgcaa aaccggtggt cgtctgctga tgtttggcgt gggcgtgatt
    33241 gtaatcggcg ttatgctctc catcttccag caatttgtcg gcatcaatgt ggtgctgtac
    33301 tacgcgccgg aagtgttcaa aacgctgggg gccagcacgg atatcgcgct gttgcagacc
    33361 attattgtcg gagttatcaa cctcaccttt accgtactgg caattatgac tgtggataaa
    33421 tttggtcgta agccactgca aattatcggc gcactcggaa tggcaatcgg tatgtttagc
    33481 ctcggtaccg cgttttacac tcaggcatcg ggtattgtgg cgctactgtc gatgctgttc
    33541 tatgttgccg cctttgccat gtcctggggt ccggtatgct gggtactgct gtcggaaatc
    33601 ttcccgaatg ctattcgtgg taaagcgctg gcaatcgcgg tggcggccca gtggctggcg
    33661 aactacttcg tctcctggac cttcccgatg atggacaaaa actcctggct ggtggcccat
    33721 ttccacaacg gtttctccta ctggatttac ggttgtatgg gcgttctggc agcactgttt
    33781 atgtggaaat ttgtcccgga aaccaaaggt aaaacccttg aggagctgga agcgctctgg
    33841 gaaccggaaa cgaagaaaac acaacaaact gctacgctgt aatcttcctg tccagcacgc
    33901 cgcgccattt cggcgtgctg tctttttact cccgcttcag ccgtttcgaa ttacacagcc
    33961 acagggtgat caccagtaac aggatcgcag ccgagtagat caacacatcc agtggcgatt
    34021 tatgatcgac gatgatcaag cgcacaatgg cggtgatccc aatatagaca aagtaacgta
    34081 gcgggaagtg aaaaccggac tgaaagtact tcacaatcag cgcgataaat tcgaaataga
    34141 gaaagtaaac caccagtcct tctaccagct catatttgct ggtttgttct ggcgcgaaca
    34201 gcacatcagc cagatgcacc gtttctttgc cgaggaagac aaccaaaatc aggccgaggc
    34261 acagcaggcc aagattgagt acggtctgca aaatggtgga gataaactcc acgcgcggac
    34321 gagagagtga cgtcatagca gtacctcctt cacagtggcg agcttcctgt ataacgcaaa
    34381 aatgtgacgg agatctatat tttttgtgta tgttttgttc aacctggatt tggagcgcgc
    34441 ctccaccaaa gccggatata cctacccgta ggccggataa gccgcgcctg gcggcgcatc
    34501 cggcaagaca aaccgcacca aagccggtaa ttcaaaaatc acccattcag gtgattccca
    34561 gccaccagca atgcgcgaaa aatcgacaat gttcctctgg aacaaatgaa acgtaacctg
    34621 ataaaggtgg taaaaatgaa gccttatttt gctgctttga tgttatcagt ctctgttctt
    34681 cctgcttatg ctggtccgct cggtactgct gataaagccg acctaccgca aagcaatgtc
    34741 tccagcccaa tgatggccca gtcgctcagg caaccagacc tgcaaccgat atcgacggac
    34801 aggaagacag aatgttttcg cctttacaca ccagatcgta aaccgggggt gaactgcgtt
    34861 cctgatggct caacaggtca ttaagaatga gggtgccagt gagtgcgaaa actggcacct
    34921 ttcccatgac ttagcggaaa tttacgctac ggtcgctggt catgtcatac aactggaact
    34981 tacgcccaag ttgctgacca ccgtcacgcg tcagcggcgt ccagccaatc gccgcacggc
    35041 tgcgtgtcgg gccagacgag aagagatcca acggtaccga gacatacacg cctttggtga
    35101 agtccccttc gccgtactcc tctttcgaaa cgttggtgat cgtggcatag ccacccacca
    35161 ccacgccgct gtcaaagcgt ttagcgatct ccagcgtgcc gcctttatcc cctgccagat
    35221 actgcccgac gctggctttc accaacacgt cctgggcgaa agatggcgtc cagtaggcgg
    35281 tcagatgtcc ggttttcacg ctgtagtcgg tgaatttcat catatctttt gcactacgcc
    35341 agtcgcgctg tttaacgtag ttggcatcca gaccaaacgc ccagttactg tccagcggac
    35401 gatacaacac ttcggccccc gcaccgccaa acatggtttc gagataacct ccatagacct
    35461 gaccgtagaa gccgttgccc agatgctgga agtagttggc ttgcaggtta ttcacataga
    35521 catcgttctg cacatactcg cgcacatggg tacgaacgcg cggtaagtgc gagtcctgcg
    35581 gtggattggt gtagttaaac ttgtcgtagt tgttggcaag atttgcaaac aggctgccgg
    35641 tggtcagtag atggtccgtc agccacaaat ctgccgttcc catcacgccc agctgataca
    35701 tgtaaaagtt ttccgggcca ccgaccgact ggttcagcac cggatcgata tggaaatcaa
    35761 agcgtgattt gtcgatatac cagccctgct cggtggactt cggaaccact ggctcgacgc
    35821 gtttgtgcgc cagcgtcgtt tcgtggccca acggctctcc ggcgagatga tttttcaggc
    35881 tggcgacatc ggtttccgtc gtcacttgcg gcatgttaag gcgattttcc gtaatgcgga
    35941 tcgttttgat cccatccggc agatcgttca tcacgatccg attagcacgg atgatccctt
    36001 cgcgcgaatc acgatatttc acctgctcgc cggtaacata cagcgtatcg cctttcgcct
    36061 ggatctgcgg atcggcaagt ccggcgttgt atttcagcaa cgttaactga ttcgccacca
    36121 ccgaatgctg caaaatggca tcctgcggct gcggttgata ttgcgggcgg gcgttatcga
    36181 tgtaggacgg gcgcagatcg ttaaagttgg tgcgcagcgt gacgccaaac ataaaggtgt
    36241 tgccacgttc atagctaagg ttaacgtcgg cccaatcggt aacgcgataa atcgccccga
    36301 cgttaaactt acttttttgc tccagcttcc cggcaaaatc ctgctgataa ttattacctt
    36361 catactccag tttcagacgc agcggttgcc agggcgtctg gtattccacg ccgccaaaca
    36421 gtgaggcagg accgtggaac atctggctac cgtcgataga tcccgcctgt ttgtagctgt
    36481 tatcgcgata gcaatattta tcactggctg aacagagcgg attttttaca ttaccgctgg
    36541 tgcccagata tccccagccc aggccgagcg taaaatcgaa cggcccccag gctttgctgg
    36601 caacaagata ttccgcatca aacagccccg taccgccgat atcccgcgca ccaaccgcca
    36661 cttgcggcag ccagtaactc tcttcccaca aacgcagttt gagatcgaag gctttatctt
    36721 tatacgtttg atcgccagag aacgcttcga cgctgctgta ctgccgggtg cgcacgtcgg
    36781 tgtagcgcag cgttgtttcc agccacggga agagttgcac tgaagctgag taataacggt
    36841 actgatcgtt atcgcgatag ttcagactca actccccttc ccgtgccata cgcgcggtgg
    36901 gagtttgtaa taatcctacg ccaccgaaat ccgactgcga cggaccaatg ggcgcaggat
    36961 atgtttcgcc gtagcaagct gtgctaatgc ccagcgccag taagctaagc agatgtcttt
    37021 ttttcattat tggggtatcc gctgcgtcag agtctgaaga atgtcggcgt taagggcatc
    37081 aggcgtctca ctccagacgg aatctgcgag gccaacataa ataatgctgc cgggcatcgg
    37141 ctctacgtga cgcttgttcc agtaagccac cggcgctttt tgcgtgcgtc cgtccgggta
    37201 aacgacccac gcgtagctgc gatccgcacc gctgagcagg ctctggtcag agagatagct
    37261 cgccacgtcg cgacctggag tgaatggctg attgccagga tggctgataa gcccgaacaa
    37321 cgtgaccgtg gacggcggtg gtccgaccca cagcgtgtag ttgccctgca acggcgggtt
    37381 accgcgttcg gcaacgcgga cgatatcggg atcaagattg attttttgcc tgcctgtcac
    37441 tttcaatgcc tggatttgct ggcgtaaggc gttaatggcg gcggcatcgt cggtgctgga
    37501 atctgcgccc tgttctgcca gccgtgtcag caacgcctgc tgctgacgca atgctgccgc
    37561 cgttgccagc tcttcgctaa tcaccgcacc aggccaccag ctgtttgcca gtcgaggttg
    37621 ccctaccaga tcgattaaat gctctgcgcc cgttagcgtt ttggcctcac tgctgccatt
    37681 gctgaacacc ttgacggttc ctgccgcgaa gaccgatgac gctcccacac tcaaaagcaa
    37741 cgcgacaata gtttgtttaa tcattgtttt gccgccttga tgagagtggt tttcaccggg
    37801 aaataatccg cgccgagata ctgttccgac tggcgaattt gcccttcgct gtcgatccag
    37861 tagcggttat gccagcgcgc ctggtcggtg gagacttctt cgtccagcac gcgaaccagg
    37921 gtttcatcgc tgccgacctt gacggtatcg gtgccatccc atttgaagac tgagcgtgcg
    37981 gtggcgtagc gtacctgctg gtactcggtc cagcccatcg tgcgcgtcca tgttgcacca
    38041 tcaacaattt gtgcgggttt aatcagcggg tcggcggcaa ggttattcac ttcaatcagg
    38101 ttgtcgccgc caagcaaagt cttcaccaga cgaccatgtt gtgtgacaag agtggcttga
    38161 tcctgagtga cccatttttg ttgtccgtct tcagcgaagg ccagcaccac aaacaattgc
    38221 ggcccgccat taagctgcat gtactggctg gcgtagggca tattttgaat atcatcgtcc
    38281 gtcagctgta cgcctggcgt gccgaacaga ctgtcccaca gtgaattgcc cagctcttta
    38341 gtcgtggctg aacaggcctg taatagcagg cagataagaa tgagtgcagg tcgcttcacg
    38401 actcttctcc agaggggcag aataaccaca ccggagtgtg gttatggtaa atcccttcat
    38461 acttcaagcc acagatgcaa tgactcaaat tattttgggg atacatactt tattactggg
    38521 tactggtggt cgtggttgtg gtggtcccgg tattagaacc atcaccgcca ccggttgccg
    38581 cgagcgcgac acccaccgcc gagcttacgg tgctgacgct ggtcgcggag gaactccccg
    38641 ccgaaaccga cgttgctgcc gactctgccg cttcgcccac ctgtacaggt gcagcccacg
    38701 cagaagtcgc cgcaagcgca gatatggcaa aaatgccata cagaactttt ttcataacaa
    38761 tttcccttca ttgaatgaat ggagatttac ccaaaaacat ttcgggcggg atcgagtata
    38821 cacaactaaa gcatgcgaat tacggcgcgg ggaattagcg atggagtttt aggataattg
    38881 gaatgaatat caaatataag tttcaactta tatttaaata acaataaaaa tcataagctt
    38941 aaaataaaaa acaaaagtaa ttaatctgcg tattctccga aataaacaac gattaaaaat
    39001 ctattttagg attaacctca cggtatgatt tccgttaaat cacagacaag gggatttatc
    39061 tgataaaaca atgccgactt attgtcggca ttgtttttaa ggttatttaa ctcctcatac
    39121 tatgcattaa tgtcgatatt ggcatcaggc tccggttgcc ggatgcggcg taaacgcctt
    39181 atccggccta catatcggca tcaggcacgc atgatgatta accgcgccac gctttatagc
    39241 ggttaatcag accattggtc gagctatcgt ggctgctgat ttctttatca tctttcagct
    39301 ctggcagaat acggtttgcc agttgtttac ccagctccac gccccactgg tcgaaggtga
    39361 agatgttcag gatcacgccc tgagtaaaga ttttgtgctc atacagcgca atcaacgcac
    39421 ccaggctgaa cggagtgatt tcgcgcagca ggatggagtt ggtcgggcgg ttaccttcga
    39481 acactttgaa cggcaccacg tagtcaagcg ttgccggatc tttaccctga tcgcgatatt
    39541 cctgctcaac cacttcgcgg gatttaccaa acgccagcgc ttcggtctgg gcgaagaagt
    39601 tagacagcag tttctggtgg tgatcagaga gcgggttatg ggtgatagcc ggggcgatga
    39661 aatcgcacgg taccattttg gttccctggt ggatcagctg gtagaacgcg tgctgaccgt
    39721 tagtgcctgg ttcaccccag ataatcgggc cagtctggta atccacaacg ttaccgttac
    39781 ggtcaacata cttaccgttg gactccatat tgccctgctg gaagtacgcc gcgaaacggt
    39841 gcatatactg gtcatacggc agaatcgctt cagtttccgc accaaagaaa ttgttgtacc
    39901 agatgccaat cagcgccagc aggacaggca ggtttttctc ggcaggcgtg gtggagaaat
    39961 gcttgtccat tgcgtgtgcg ccagaaagca gttcaacgaa gttatcaaag ccgatggaga
    40021 gaacaatcga caggccaatc gctgaccaca aagagtaacg gccgccgacc cagtcccaga
    40081 actcgaacat gttggcagta tcaataccaa actcgccaac ggctttggca ttggttgaga
    40141 gcgccgcaaa atgtttcgca acgtgttttt catcacctgc cgctttcagg aaccagtcac
    40201 gcgcgctatg ggcgttggtc atggtttcct gagtggtgaa ggttttagat gctaccagga
    40261 acagcgtggt ttccgggttt acttttttca gcacttccga gatgtgagtc ccatcgacgt
    40321 tagaaacaaa gtgcatgttc aggtggtttt tgtacggacg cagtgcttcg gtcaccatgt
    40381 acgggccgag gtcagaaccg ccgatcccga tgttcactac gtcagtgatt gctttgccgg
    40441 tataaccctt ccactcaccg gagataatcg cttccgagaa ggttttcatc ttctccagca
    40501 ccgcgttgac ttctggcata acatctttgc catcaaccag aatcggggta ttgctacgat
    40561 tacgcagcgc tacgtgcagc acggcgcggt tttcagtgcg gttgatcttc tcgccagaga
    40621 acatcgactt aatcgcgccc gccagatcgc actctttcgc cagatcctgt aatttcgcca
    40681 gcgtctcttc agtgatgcgg tttttggagt aatccaccag catctgatcg tcgaaggttg
    40741 cggagaactt agaaaaacga tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt
    40801 tcatttcatc gaagtgtttc tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt
    40861 ttttcattag caatactctt ctgattttga gaattgtgac tttggaagat tgtagcgcca
    40921 gtcacagaaa aatgtgatgg ttttagtgcc gttagtgtaa tgttgagtgt aaacccttag
    40981 cgcagtgaag catttattag ctgaactact gaccgccagg agtggatgaa aaatccgcat
    41041 gaccccgtcg ttgacaaccg ccccgctcac cctttattta taaatgtact acctgcgcta
    41101 gcgcaggcca gaagaggcgc gttgcccaag taacggtgtt ggaggagcca gtcctgtgat
    41161 aacacctgag ggggtgcatc gccgaggtga ttgaacggct ggccacgttc atcatcggct
    41221 acaggggctg aatcccctgg gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt
    41281 ggtggagcac ttctgggtga aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc
    41341 gctcttccct tgtgccaagg ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa
    41401 attgttgtct ccaaatttgg cggtaccagc gtagctgatt ttgacgccat gaaccgcagc
    41461 gctgatattg tgctttctga tgccaacgtg cgtttagtag tcctctcggc ttctgctggt
    41521 atcactaatc tgctggtcgc tttagcggaa gggctggaac ctggcgagcg attcgaaaaa
    41581 ctcgacgcta ttcgcaacat ccagtttgcc attctggaac gtctgcgtta cccgaacgtt
    41641 atccgtgaag agattgaacg tctgctggag aacattactg ttctggcaga agcggcggcg
    41701 ctggcaacgt ctccggcgct gacagatgag ctggtcagcc acggcgagct gatgtcgacc
    41761 ctgctgtttg tcgaaatcct gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt
    41821 aaagtgatgc gtaccaacga ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg
    41881 gaactggccg cgctgcagct gctcccacgc ctcaatgaag gcttagtgat cacccaggga
    41941 tttatcggta gcgaaaataa aggtcgtaca acgacgcttg gccgtggagg cagcgattat
    42001 acggcagcct tgctggcgga ggctttacac gcatctcgtg ttgatatctg gaccgacgtc
    42061 ccgggcatct acaccaccga tccacgcgta gtttccgcag caaaacgcat tgatgaaatc
    42121 gcgtttgccg aagcggcaga gatggcaact tttggtgcaa aagtactgca tccggcaacg
    42181 ttgctacccg cagtacgcag cgatatcccg gtctttgtcg gctccagcaa agacccacgc
    42241 gcaggtggta cgctggtgtg caataaaact gaaaatccgc cgctgttccg cgcgctggcg
    42301 cttcgtcgca atcagactct gctcactttg cacagcctga atatgctgca ttctcgcggc
    42361 ttcctcgcgg aagttttcgg catcctcgcg cggcataata tttcggtaga cttaatcacc
    42421 acgtcagaag tgagcgtggc attaaccctt gataccaccg gttcaacctc cactggcgat
    42481 acgttgctga cgcaatctct gctgatggag ctttccgcac tgtgccgggt ggaggtggaa
    42541 gaaggtctgg cgctggtcgc gttgattggc aatgacctgt caaaagcctg cggcgttggc
    42601 aaagaggtat tcggcgtact ggaaccgttc aacattcgca tgatttgtta cggcgcatcc
    42661 agccataacc tgtgcttcct ggtgcccggc gaagatgccg agcaggtggt gcaaaaactg
    42721 catagtaatt tgtttgagta aatactgtat ggcctggaag ctatatttcg ggccgtattg
    42781 attttcttgt c
//
LOCUS       DAJVQR010000027        40370 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVQR010000027 DAJVQR010000000
VERSION     DAJVQR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..957)
                     /locus_tag="OQM52_001523"
     CDS             complement(<1..957)
                     /locus_tag="OQM52_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCS1563423.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1203..1829
                     /gene="gstB"
                     /locus_tag="OQM52_001524"
     CDS             1203..1829
                     /gene="gstB"
                     /locus_tag="OQM52_001524"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCS1563424.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2940)
                     /gene="yliI"
                     /locus_tag="OQM52_001525"
                     /pseudo
     CDS             complement(1826..2940)
                     /gene="yliI"
                     /locus_tag="OQM52_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3051..3434)
                     /gene="bssR"
                     /locus_tag="OQM52_001526"
     CDS             complement(3051..3434)
                     /gene="bssR"
                     /locus_tag="OQM52_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1563425.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3647..4972
                     /gene="rimO"
                     /locus_tag="OQM52_001527"
     CDS             3647..4972
                     /gene="rimO"
                     /locus_tag="OQM52_001527"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1563426.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5019..6347)
                     /locus_tag="OQM52_001528"
     CDS             complement(5019..6347)
                     /locus_tag="OQM52_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1563427.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6355..8697)
                     /locus_tag="OQM52_001529"
                     /pseudo
     CDS             complement(6355..8697)
                     /locus_tag="OQM52_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8875..9786)
                     /gene="gsiD"
                     /locus_tag="OQM52_001530"
     CDS             complement(8875..9786)
                     /gene="gsiD"
                     /locus_tag="OQM52_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1563428.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9789..10709)
                     /gene="gsiC"
                     /locus_tag="OQM52_001531"
     CDS             complement(9789..10709)
                     /gene="gsiC"
                     /locus_tag="OQM52_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1563429.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10727..12265)
                     /gene="gsiB"
                     /locus_tag="OQM52_001532"
     CDS             complement(10727..12265)
                     /gene="gsiB"
                     /locus_tag="OQM52_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1563430.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12285..14156)
                     /gene="gsiA"
                     /locus_tag="OQM52_001533"
     CDS             complement(12285..14156)
                     /gene="gsiA"
                     /locus_tag="OQM52_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1563431.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14143..15108)
                     /gene="iaaA"
                     /locus_tag="OQM52_001534"
     CDS             complement(14143..15108)
                     /gene="iaaA"
                     /locus_tag="OQM52_001534"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1563432.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15312..16547
                     /gene="moeA"
                     /locus_tag="OQM52_001535"
     CDS             15312..16547
                     /gene="moeA"
                     /locus_tag="OQM52_001535"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1563433.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16547..17296
                     /gene="moeB"
                     /locus_tag="OQM52_001536"
     CDS             16547..17296
                     /gene="moeB"
                     /locus_tag="OQM52_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1563434.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17475..18137)
                     /gene="fsa"
                     /locus_tag="OQM52_001537"
     CDS             complement(17475..18137)
                     /gene="fsa"
                     /locus_tag="OQM52_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1563435.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18268..19167
                     /locus_tag="OQM52_001538"
     CDS             18268..19167
                     /locus_tag="OQM52_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1563436.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19173..21605
                     /locus_tag="OQM52_001539"
     CDS             19173..21605
                     /locus_tag="OQM52_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1563437.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21751..22566
                     /gene="ybiV"
                     /locus_tag="OQM52_001540"
     CDS             21751..22566
                     /gene="ybiV"
                     /locus_tag="OQM52_001540"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1563438.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22718..23983
                     /locus_tag="OQM52_001541"
     CDS             22718..23983
                     /locus_tag="OQM52_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1563439.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24224..25816)
                     /locus_tag="OQM52_001542"
     CDS             complement(24224..25816)
                     /locus_tag="OQM52_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1563440.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26035..26955
                     /gene="ldtB"
                     /locus_tag="OQM52_001543"
     CDS             26035..26955
                     /gene="ldtB"
                     /locus_tag="OQM52_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1563441.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27013..28131)
                     /locus_tag="OQM52_001544"
     CDS             complement(27013..28131)
                     /locus_tag="OQM52_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1563442.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28128..28595)
                     /gene="mntR"
                     /locus_tag="OQM52_001545"
     CDS             complement(28128..28595)
                     /gene="mntR"
                     /locus_tag="OQM52_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1563443.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28781..28909
                     /gene="mntS"
                     /locus_tag="OQM52_001546"
     CDS             28781..28909
                     /gene="mntS"
                     /locus_tag="OQM52_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1563444.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29181..30764
                     /gene="opgE"
                     /locus_tag="OQM52_001547"
     CDS             29181..30764
                     /gene="opgE"
                     /locus_tag="OQM52_001547"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1563445.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30812..31327)
                     /gene="ompX"
                     /locus_tag="OQM52_001548"
     CDS             complement(30812..31327)
                     /gene="ompX"
                     /locus_tag="OQM52_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1563446.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31380..31445)
                     /locus_tag="OQM52_001549"
     CDS             complement(31380..31445)
                     /locus_tag="OQM52_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563447.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31680..32567
                     /gene="rhtA"
                     /locus_tag="OQM52_001550"
     CDS             31680..32567
                     /gene="rhtA"
                     /locus_tag="OQM52_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1563448.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32866..33369
                     /gene="dps"
                     /locus_tag="OQM52_001551"
                     /gene_synonym="pexB"
     CDS             32866..33369
                     /gene="dps"
                     /locus_tag="OQM52_001551"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1563449.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33773..34519
                     /gene="glnH"
                     /locus_tag="OQM52_001552"
     CDS             33773..34519
                     /gene="glnH"
                     /locus_tag="OQM52_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1563450.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34658..35317
                     /gene="glnP"
                     /locus_tag="OQM52_001553"
     CDS             34658..35317
                     /gene="glnP"
                     /locus_tag="OQM52_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1563451.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35314..36036
                     /gene="glnQ"
                     /locus_tag="OQM52_001554"
     CDS             35314..36036
                     /gene="glnQ"
                     /locus_tag="OQM52_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1563452.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36297..38521
                     /gene="ybiO"
                     /locus_tag="OQM52_001555"
                     /pseudo
     CDS             36297..38521
                     /gene="ybiO"
                     /locus_tag="OQM52_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38518..39444)
                     /gene="rlmF"
                     /locus_tag="OQM52_001556"
     CDS             complement(38518..39444)
                     /gene="rlmF"
                     /locus_tag="OQM52_001556"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1563453.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39720..39980
                     /locus_tag="OQM52_001557"
     CDS             39720..39980
                     /locus_tag="OQM52_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1563454.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
BASE COUNT        10052 a        10664 c         9826 g         9828 t
ORIGIN      
        1 ggagcccgct tcgcctgccc cgagattcac ttcgatctta tcaccaaacc agacgcgctg
       61 agtcacaaag gtggcatcag gtttaattgg cgtcacagtt tcaaagaagc ggaaacccca
      121 ggtcaataat ttctcagact cattaaaacg gatacggtca gttttcgccc ccagcactac
      181 ggagattaaa cgcatatcac cctgggtagc cgaagcaacc agattatatc ccgcacctgc
      241 cgtggttcct gtcttcatgc catcaacatt cagattgctg ctccacagca gacggttacg
      301 gttaggctga cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg cgtactcttc
      361 cggcacatcg tggatcaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg
      421 ccccggcgca tccaggccgt gcaccgtctg gaaggtagtg ttggtcagcc ccagtttttt
      481 ggcataacca ttcatcagac caataaagga ctcctggctc ccggcaacgt aatctgccag
      541 cgcaatacag gcgtcattac cggactggat aatcacacct ttgttcaagt ctgccaccga
      601 aacctgatcg cccggtttga ggaacattac cgatgaacca cgcagtgccg gatttcccgt
      661 cgcccaggca tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag
      721 cgcctgccca accacatagc tggtcatgat tttagtcagg ctcgcaggat ccagtttctc
      781 atccgcattg ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg
      841 cgcatccacg ctcggcgctt caacggtttg ttcggccgcg aatgccgttg gggcaaaaag
      901 gaataaaaat gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa
      961 tgccacccga gtatccattc aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagctccc aataaataat ttttgctgcc actgggggaa
     2041 tttatcgctg ttatagaagg ccatgccgct cacagcgggc gaatctttcc agtaaaaaac
     2101 aggttgctcg gtcccggcga cgatctcccc tttcgcttcc ggtatcttaa agcctgaata
     2161 gttgattccc caggttgcca gcggccagcc gtagtttttg cctttttgcg ggatattaat
     2221 ttcatcacca ccgcgcgggc catgttcatt cagccacagt gcattactcc acggattcat
     2281 cgccattcct tgcggattac gaatgccata agaccagatc tcggcgcgcg caccggattc
     2341 ctttataaaa ggattatcat ccgggatttc gccctggtcg gtcagacgca ccagtttgcc
     2401 ctgtaattta tccagatcct gcgccgtcgg gcgctgattg ttttcgccca gagcaataaa
     2461 aagataacct ttaccgtcga ataccagccg cccgccaaaa tggttgccgg tagacagttt
     2521 tggcatctgg cggaagacgg tgcggaagtc ggtcactttt gagagatcat cacttaagcg
     2581 gccataaccc acagcagttc cggctttgcc atcatcgcca acttcggaat aacttaacca
     2641 gatgcggcga gactgagcaa aatcaggcgc taaaaccacg tccagcaggc cgccctgccc
     2701 gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat aatccttttc ctgcttgcca
     2761 gtgacgcaac tcgccgccgc gcagagtgat taacataccg tgattatcgg gtaaaaaggc
     2821 cagtgcccag ggatggtcga gtttgtcttg cagtacttcg acatttaccg ttgcaggagc
     2881 cgcccagaga gcggaagaaa gacaaataag gggcacaagg aaaaaggatt gtcgatgcat
     2941 aacgcctccg gattgactct ggcttaaagc gtagtcagtg gaggagataa caaattcatt
     3001 tttacaaaaa cttaaacatg aagggggaga cgctttctcc cccttagttt tcaggccttc
     3061 tcaagcatgg cgtgcttctg caggctctgg atactcagcg ttaagctcat cagacaattt
     3121 tcaagcttat cggcgttgac ggtaataaca gtcgggcaat catggtgccc actcatcaaa
     3181 catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca ctcttcctga
     3241 tccagatgaa gattcaaccg cagcgattta tcgtgcagtt cgcgattcag tttaaaaaag
     3301 ttatctcgta gatgattgct ttcgctgacg gacatgtatc cttttgcctt cctcagttgc
     3361 acgtatgcag cgaggtcaac gcgtgcgtcg atcaaccggt tcagcagatc gattcgctgt
     3421 ctgtcaacga acatacttcg atcctcctct ttccagcctt gttgcaatta aatagtatgg
     3481 ctattttttg agcaaaatgc tgtccgggat caattcttca cgccgaatta acaaaattta
     3541 catttccttc gcctccgcca gcgaatgaga ttgcgataca aattttatcc agaactctgt
     3601 agaatccctg ccctgacctt tcctgtaact gaatattttt taagctatga gcaaagtaac
     3661 tccccagccg aaaatcggct ttgtttccct tggctgtccg aaaaaccttg tcgattcaga
     3721 gcgtattctc accgaactcc gcactgaagg ttatgacgtg gtaccgagct atgacgatgc
     3781 gaacatggtg atcgtcaaca cctgcggctt tattgacagc gcggtacaag aatcactgga
     3841 agccattggt gaagcgttga atgaaaacgg caaggtaatt gtgaccggtt gtctgggggc
     3901 aaaagaagat cagatccgcg aagtccaccc gaaagtgctg gaaatcaccg ggcctcatag
     3961 ctatgagcag gttctggagc acgttcatca ctacgtgcca aaaccgaaac acaacccatt
     4021 cctgagcctg gtgccagaac aaggtgtgaa actgacgccg cgtcattatg cctatctgaa
     4081 aatttcggaa ggctgtaatc accgctgcac cttctgcatt attccgtcta tgcgcggcga
     4141 cctgatgagc cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg tagatgcggg
     4201 cgttaaagag attctggtga tctcgcagga tacttccgcc tatggcgttg atgttaaaca
     4261 tcgtactggc ttccacaacg gcgagccggt aaaaaccagc atggtcagcc tgtgcgaaca
     4321 gttatcgaaa ctggggatct ggacacgtct gcactacgtt tacccttatc cgcatgtgga
     4381 cgacgtcatc ccactaatgg cagaaggcaa aatcctgccg tatctggaca ttccgttgca
     4441 gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag atcgccaact
     4501 ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg accctacgct caacctttat
     4561 tgtcggcttc cctggcgaga cggaagaaga tttccagatg ctactcgact tcctgaaaga
     4621 agcacgtctg gatcgcgttg gctgctttaa atacagcccg gttgaaggtg cagacgccaa
     4681 tgccctgcct gaccaggttc cggaagaagt gaaagaagaa cgctggaacc gtttcatgca
     4741 gctgcagcag cagatttccg ccgagcgcct gcaagagaaa gtgggccgtg aaattctggt
     4801 gattatcgac gaagtggacg aagaaggcgc gattggtcgc agcatggcag atgcaccgga
     4861 aatcgacggc gcggtttatc tcaatggtga aaccaacgtt aagccgggtg atatcctgcg
     4921 tgtgaaagtc gagcacgccg atgagtacga tttgtggggt agccgggttt aattaattca
     4981 ggccgggcaa aatcatttgt ccggcctgcg aataatgatt actgccagct aatctgtgcc
     5041 ctgccgtttt tcttactctt atacagccgc tcatccgcca gcattttcaa gcgttcgacg
     5101 gtagtacatt catttgaatg cgctacaccg atactggtgc ttaaattaat gcttcccacc
     5161 tggaaatgaa cgatttcttt tagtatcgat agcaactctt ccagcgtgcc gccagtataa
     5221 atcaccgcaa attcgtcacc tccgaagcgg tagactttac ctttatctac cgggatctgt
     5281 gatttaagga ttttcaccac tttcatcaac acttcatcgc ccagcaaatg accgaaggtg
     5341 tcgttaatgt ttttgaattt atcgacgtca aacagacaca aataaccgcc tttttccggc
     5401 agcgctttta aatcttcatc aaaggatttt ctgcccattg ctttggtcag gtcgtccagg
     5461 aacgccgagt tatacatact gtagagtttt ttgacatcgg cagccgaaaa gaaaagcccg
     5521 ccatccagta cgcgttcaaa atggttatta atgatggctt ctttctccac aataccacga
     5581 ttaatcactc tgcgtaccag atagaggcaa ctggcaccca atatcactgc caccaatgcg
     5641 gcaaagaaaa taatcaatgt acaaacccgc aaataaacat cttttttata cgcaaatacg
     5701 tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt gaagatggcg
     5761 tttttagcat tatattcatc accggtcacc ggtttaccgt tcttaaatag agagaacatc
     5821 aggttctcgc tgtccatcac cagcgatttt gacagtaaag aaaaacgctc cagaccaagc
     5881 aaaaatatca gactacgatt gtgagttacc ggatagaggt aatagacgaa ggttttaccg
     5941 tcaaaagaga taaaccctgt atttttgaca tgtcgatact tcacaacctg cataatatct
     6001 gcacaataat ttttctcgaa ctcctttacc tgaatacatt cgtcactgga aatcagataa
     6061 ggaccgacat cgtacaaata agagggggaa accgagtcgg cgtaagctgt gcgcaaacca
     6121 accaacagct gttcctccgt gggatttagc gtgttgataa gatcaattgc cacgctacgc
     6181 cgatattcag caatatttgc caccgcaaaa gcagtatcaa taacggactg tttcaggcta
     6241 tacacccgtt cctttaccat ttgagtgtag atcccgcagg caactgctga aatcatgata
     6301 aaaatcagca gactgactgt aagtacgaac ttattgattc tggacatacg taaactactc
     6361 ttttactaat tttccacttt tatcccaagc ggagaatggc atgggtttct ggtacagata
     6421 cccctgagcc tgaacacatc ctaatttaat taattcgcgc gcaacgtctt cgctctctac
     6481 gccttccgca atcaccggga tccggttata gcgtgccaga tcgaaaataa tgcgcacaat
     6541 ttttcccgca gtaccgaaaa tatcgccggt aaattgcgag tccaccttta tgctgtctgg
     6601 attaatttca taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat caatcgatat
     6661 ccgtacccct tgtttacgca gttgttcaat attgagataa aaatgttcat taattcgctc
     6721 gatcacggtt tcagtgatct caacacagag gctgttcgcc ttcaggccat tttctttcat
     6781 gatggtggta aaacgctcat gaaagtgcgg ttcgtttaac tgccagggcg aaacattaat
     6841 atgcagtaag aaatcatcct tcagcgacgc acgttgacta aaatgtctga attaacccat
     6901 tgccgtttta ataatctgat aacccagctc attgataaac ccgatatctt cagcaatggt
     6961 aatgaacttc agcggtgaaa tgatcccccg ctccgcagaa acccaacggc acaacgcctc
     7021 cgcgctcaga atggcgcggg tgttaatatc aacgatcggt tgatagacga ggaatagttc
     7081 gccaatttcc attgcatggc gaatattacg cgcaatccag atattctctt ctatttcgtt
     7141 gagtttcgcg tcatcacaga aaataaactc ggagaacata tggtcacgga tactggaaat
     7201 cgccagtttc agtttttctg cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat
     7261 ggcagactta tggatgcgca gattgcggta gccaaagctg ctgtcaaaat cgtttaccat
     7321 cgctatttta cgggtaaatg tctgtacatt ttcccgacac agaagtaaat agagatcggc
     7381 attgaatgtc acgatatcgc agcattgcgc atatttctcg gcaattcgct gacacaattt
     7441 cctgacgatt ttattgcgtt catcaacacc aaacaccgcc tccacggctt ccagaccaca
     7501 cagtttgatg atccccacag ccagatactg attattgttg gactcaataa tcgccatatt
     7561 tttaataaaa ccgctttcct gatcgtaaga gatcagtgag agcaatttat ttgaccgttc
     7621 aatttcggca tctttcattt ccgcgatcga atttttgatg ctgataatct ctttcgcatc
     7681 gccttcgcga attttcagag cctcgatctg atccgttgag tcgggcaaaa acgccagcgt
     7741 attaaccagc tcggtcattt cagaaaattg ccgacggaac acacgagtgg tcatgaaata
     7801 taccaccagc aacacgataa taatgaacgc aaacggtaga taaaaaagtt tatctgatgc
     7861 gttcaataaa ttaccgccag gataataaaa atagatttcg aacaatgcat tatttggcac
     7921 tgctgatgtc tttgcaatat actccgtatc gacagaatca ataatattat aattaccttc
     7981 gctattataa ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatgacc
     8041 ttgtttcaaa aatagaactt catcattagc gctgatttga tcaagatact cgttcagata
     8101 ccgccagttt aaatcgacat gaattttatc taccgctaac aaatcgtgat ctttgccgtt
     8161 gatacgtagc gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtatgccgg
     8221 attccagctg ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa
     8281 atcggcaaaa ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg
     8341 ataaacaacg attttatcat tcgccctccg cgaaaagaga taatatttat tatcatcaag
     8401 taccagtccc accgagtcta aaaatggcat catttccatt ttcagaatca cggcgcgttg
     8461 aatctcctct gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc
     8521 aatatcttta aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg
     8581 ttttaccgcg cgttccgata ccagttgata gttaatcatg aaaaaaagac caataaatga
     8641 cagtgctgcc aataaaaata aaattatcag ccttatcttt tcgtataaac tcagcatgtt
     8701 aatacattta acaaaataac tatctgaacg ctgtgccgct gcaaaaatag tcccttgctg
     8761 cggcttttta accaaattac ccgattctac aaaatcagca gaaagttaca acacttgctt
     8821 agggcaataa tttcctgtga ggtgattacc ctttcaagca atattcaaac gtaactatcc
     8881 tttaattttc ggatccagcg catcgcgtaa accatcgccc aacaaattga acgccagtac
     8941 ggtcagaaaa atagccaggg ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc
     9001 tcgtgcctca ttgagcattg ctccccactc tggtgtcggc ggctgcgcac cgaggccgag
     9061 aaatgacagg ctggcggcag agataatcga ggtgccaatg cgcatggtga aaaacaccac
     9121 gatagaagag acggtcccag gcaggatatg acgcaataaa atggtcatat cgctggcacc
     9181 aatactgcgt gctgactcaa taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac
     9241 caggcgggca aacgcgggga tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc
     9301 gcttcccaac accgcaacaa cagcgatcgc cagtaaaata cccgggaagg caaacagcac
     9361 atcgcaaatg cgcatgatca gccgatccca ccagccttca taatatccag ccaacaagcc
     9421 cagcaacgtc cctatcgcca caccgataaa cacggcaaac acgcccgccg ccagcgagat
     9481 ttgcgcacca accaggacac ggctgaaaat gtcgcgcccc agtgaatcga cgccaaacca
     9541 gtgctgcaaa gaaggtccgt tattcagatt gtcataatca aaataatttt cggcgtcata
     9601 gggagcgatc cagcgtgcaa aaatggccac cacaatcaat aaaataacga ataatgcggc
     9661 ggtcatcgcc atatgctgac ggcgaaatcg tcgccagaat tcatgccacg gtgtacgtac
     9721 ctggtcaggt ttgaccagtg gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag
     9781 tcgcatcctt acttgtacct gatagccggg ttaatggcgg cgtaaagcac atccaccact
     9841 aagttgataa gaataaattc cagcgagaaa agcagaattt ccgcctgaat caccgggtaa
     9901 tcgcgcattt ctacagagtc aacgagtaag cgtccaagtc cgggccagtt gaaaactttc
     9961 tccaccacga tagaaccacc gagcaaaaag ccaaactgta agcccatcat ggtcactacc
    10021 gggatcatcg cgttgcgtag cccgtgtttg aggacaaccc aggtttcgct cacccctttc
    10081 gccctcgcgg tacgcatata atcttcgctt aaaacgtcga caaatgacgc acgggtaaag
    10141 cgcgccatca cggcggccac tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc
    10201 caactgtctg ctcccacagt aggcagccag cccagttcaa cggagaatac ctgaattaaa
    10261 aacatcccca gcgcaaatgc cggaaacgag atccccgaca ccgcaatggt catactcaat
    10321 cgatccggcc aacgattacg ccagacggcg gcgataattc ccgccgccat accaaatatc
    10381 accgcccaga ccatactggt tatggtcagc catagcgttg gcataaagcg gctggcaatc
    10441 tcatcggcaa ccggacgacg cgacaccatc gacaggccaa aatccccctg cacagcattg
    10501 ctgatatagt gccagaactg gtgatacagc ggctgatcca accccagctg ctgacgcacc
    10561 agttctataa cctgcgcatc agcttcgggc ccggcaatca atcgcgccgg atcgccgggc
    10621 agcatgtgga caaataaaaa caccagcacc gagacgataa acagcgtcgg aatcaacccc
    10681 agtaatcgtt tgataacgta ataaagcatt ccactccctg cgttgcttat tgcaaatccg
    10741 cgtcttcaaa gctgaagccg gtatctggca tgatccaaaa accggtcagg tttttactgt
    10801 gtgccgacac cagtttttct accaccagcg ggatccacgg cgattcttgc cagatgatat
    10861 cctgcgccgc cttatataag cgggtctttt ccgccggatc attcgttttc agtgcctgag
    10921 ccaggaagtc atccacctgt ttattgctgt aaaacgcggt attaaacagc gtcggtggcc
    10981 agttttgcga ggcaaacagc ggcgatagtg cccagtcggc ttcgccggtt gaagccgacc
    11041 agccagtgta gaacatccgc acgccactct ctttttgccc tttaccttca acttctgccg
    11101 cccgctgtcc ggcatccatc gcggtcacct gggctttaat cccgacctgc gctaactgct
    11161 gctgggtaaa ttgcagcact ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg
    11221 tactgaaacc gttgggatat cccgcctctt tcagtaattc gcgcgctttc actggatcgt
    11281 aaggccacgg tttataactt tgcgcgtagg cgatacttgg cggtaccaca ccagtagctg
    11341 gagttgcata gcccgcaaag gcaactttca ccagcgccgg acggttaatg gcgtaattca
    11401 gcgcctcacg gaccttcggg ttatcgaacg gcttttgcgt cacgttcata ctgatataac
    11461 gctgcataat tgacggactg gccatcaact cgatattttt gtttttctcc agcagtgtgg
    11521 cctgctcgta gggaatgggg aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc
    11581 gggtgttgtt atccgccacc ggacgccagg ttatgctgtc cagtttgggc aatcctggct
    11641 gccagtaacc cgcgaatttt ttcaccttca caaaatcggt ctgattccag gtatccagtt
    11701 catacggtcc ggttcccacc ggatgaaaac caatctcctt gccatatttt tccagcgctg
    11761 tcggtgaaat catcgcggtc gccggatggg caagaatatt aataaacgct gagaacggct
    11821 gtttgagggt aatctttacc gttgtcggat cgatcgcctc cgttttggca atattcttat
    11881 acaggttata gcgtttaaga tgattcgccg gatcgctggc ccggtccaga ttcgctttta
    11941 ccgccactgc gttgaaatcg gtgccatcct ggaatttaat tccttcccgc aatttcacgg
    12001 tgtaagtaat gccgtcatcg gaaacggtat aactctccgc cagcacgttt ttcagtttca
    12061 tctctttatc cagaccgaac agcccctggt aaaacgattt cgctacggcc tgagataacg
    12121 tgtcattcgc gtcatacgga tcgagcgtgg tgaaattcga tcctaccgcc accaccacat
    12181 ctttggcagc gaatgcacaa gatgccatca acgctgtcgc aatgcccagc gccactaacc
    12241 cactacggtg tacagctctt gccattttat tctccgcctg aatgttatct acgcatgaat
    12301 gcgtattctg attgtggttg tgcgacgtaa tgccccggcc cgacgcattg caacgagacg
    12361 gctgccacct cttcgccacg cagatgaata ttgctgggaa gatcgtccga cagcagtaca
    12421 cgctgcggtc gttgtcggga cggttcagcg accggaactg ccgccagtaa tttacgcgta
    12481 taaggatgct gcgggttttc gaagaccgcg caccgtgaac caatttcaac aatttgcccg
    12541 agatacatca ccgccacacg atgactaatc cgctctacca cagccatatc gtgggagata
    12601 aacagatacg caatgccgaa atcacgctgg agatcgagca acaagttgat aatctgcccg
    12661 cgaatagaaa catccagcgc cgaaacggct tcgtcggcaa tgatcacttt tggattcaat
    12721 gccaacgcgc gagcaatgca gatgcgctgg cgctgaccgc cggaaaactc atgcgggtaa
    12781 cgccaggcat gttcaggtaa caggcccacg cgctccagca accacgcaac gcgtgcagcc
    12841 gcgtctttac ctggcaataa accgtgtaca cgcagcggtt cgataatcga atcaccgatg
    12901 gtctgacgtg ggtccagcga agcgtaaggg tcctgaaaaa taaactgaat atcccggcgc
    12961 aatgcctgaa gtttgccagg tgacaaggta tcgattcgct gaccgttaaa gataatttcg
    13021 ccgccctgcg attcgaccag gcgcagcaac gcccgcccgg tagtggattt accgctgcca
    13081 gactcgccca ccagcgatag cgtttcgcca ggccagagat caaaactgac tttctcaacg
    13141 gcatgcactt cccgcgttac gcgattcaac aaaccgctgc gcaaagggaa acgggtgaca
    13201 agattacgca cccgtaaaac aggttcgcca tccaccaccg ttttctgctc gatggggggt
    13261 tcctgtttcg ctggatgttc aagcgatatc aatgggaaac gtcggggata atctaaccct
    13321 ttcatcgcac caagttgcgg aacagcagct aacagcgcac gggtgtaagg atgttgcggt
    13381 gcatgaaaaa tctgttcgac ggtacccgtt tccaccgcct cgccctgata catcaccagt
    13441 acccgatcgg caatctctgc caccacgccc atatcgtgag tgataaagat aacgcccatc
    13501 gacatctctt tttgcaatac tttgattaat tgcaggatct gcgcctgaat agtgacatcc
    13561 agcgcggtgg ttggctcatc ggcaatcagc accgccgggc ggcatgacag cgccatcgca
    13621 atcatcactc gctggcgcat cccgccagag agttgatgcg aataacgtga aagaatggtt
    13681 tgtgcctccg gaatgcgtac ctgatccagc atccgcttcg cctcgaccat cgcttcttca
    13741 cgactggcgt tctgatgcag acgaattgat tcggcaatct gttcacccac agtaaatact
    13801 gggttcagcg atgtcatcgg ctcctgaaat atcatcgcca tatccgcacc gcgcacatgg
    13861 cgcatttgtg cagcgctctg ctcgctaagt tcaatcacat cacgactgcg ccgccgcaac
    13921 agcattttat cgcactgtac taaaccgccc gcctgttcca acagacgcat caatgccagc
    13981 gcagtcactg acttaccgga gccggattcg cctacaattg ccagcgtctc accgcgttgc
    14041 agactaaaag agagattgcg gaccgcagct attttctgct ggtcctgcat aaaggcaata
    14101 ttaagatttt caaccgccag cacattaccg gcatcaagtt catcactgtg tggcaacggt
    14161 gtcccctttt tcacggtaga taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg
    14221 atacattcct tcggtgttaa acggtagcgc gacattccct tcatggtcga tagcgattaa
    14281 gccaccgcta ccgccaagcg cagggagttt ttccattact acccgctcgc aggcttccgc
    14341 gagacttaat ccgccgtaat ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat
    14401 gaagacttcg cctgtgccgg tacaagaaac cgccacactg gcgttattgg cgtagcatcc
    14461 ggcacccact aagggactat cgccaactcg tccgggtaat ttattggtca ttccgcctgt
    14521 ggacgtggct gccgccagat tgccgtctaa atccaacgcc acggccccca cggtgcccat
    14581 tttctgtttt tcatccagtg gcgcaccgct atggtcgagg actgttgccc cttcctcgcg
    14641 cgctgccagt agttgttcat aacgcaaagg cgtggagaaa atctccggtg agacgcgctc
    14701 catgccatga gcaaacgcaa aattttctgc cccttcgcca atcatcatca catgcgggct
    14761 ttgctccatc accagccggg cggcaagaac cggattacgc agatgactaa cgcccgccac
    14821 cgcaccggct ttcagggtgt taccatccat cacacaggcg tccagttcat gggtttcatc
    14881 acgcgtaaag acagcgccaa ttccggcgtt aaacagtgga cactcttcca gcagacgcac
    14941 cgcttccgtc accacatcca gcgcactttc gccggcttcc agcattttct gcccggtttc
    15001 aacaatggca gacaacgcct cgatgtagcg taattcctgt tgcagactca tctgcgcgcg
    15061 gctaattgca cctgcgccac catgaattgc aatgactgct ttgcccatag tattcgtcca
    15121 ttaagggtgg ctcgccactt ttgctataaa tatcagaatt tttatgaatt actcattcaa
    15181 tttttgaata tagaaagatg gatatggcat gtaaaggcag gagccaacgc tacgcccgag
    15241 gtggcataca gtctatggcg tttttctgac ataataggca aattcgattt tgcctccgca
    15301 ggagtgtttt catggaattt accaccggat tgatgtcgct cgacaccgcg cttaatgaga
    15361 tgctttctcg cgttacccca ctgaccaccc aggaaacgct gccactggtt cagtgttttg
    15421 gtcgtattct ggcgagcgat gtcgtttcgc cactggatgt cccggggttt gataactccg
    15481 ctatggacgg ctacgcggtg cgtttagccg atattgcctc cgggcaaccg ctgcccgttg
    15541 ccggtaaatc ctttgccggt cagccatacc atggtgaatg gcctgcgggt acctgcattc
    15601 gtattatgac cggtgcgccg gtgccggaag gctgcgaagc ggtggtgatg caggagcaga
    15661 ctgaacaaac ggacaatggc gtgcgtttta ctgctgaagt gcgtagcggg caaaatattc
    15721 gccgtcgcgg tgaagatatc tctgcaggtg cggttgtttt cccggcggga actcgcctga
    15781 ctaccgcaga gctgccagta attgcttcac tggggattgc cgaagttccg gtgattcgta
    15841 aagtgcgtgt agcgcttttt tctaccggtg atgaactcca gttgcccggt cagccgctgg
    15901 gcgacggcca aatctacgac accaaccgtc tcgccgtaca cctgatgtta gaacagctgg
    15961 gttgcgaggt aatcaactta gggattatcc gcgacgatcc acatgccctg cgcgccgcat
    16021 ttattgaagc cgacagccag gcggatgtag tgatcagttc cggcggtgtt tcagtgggtg
    16081 aagcggatta caccaaaatg attcttgaag agctggggga gatcgccttc tggaagctgg
    16141 cgattaaacc aggtaaaccg ttcgcgttcg gtaaactcag caatagctgg ttctgcggcc
    16201 tgccgggcaa cccggtttca gcgacgctga ccttctatca actggtacag cctttgctgg
    16261 caaaactaag cggcaacacc gccagcggcc tgcccgcgcg ccagcgcgta cgcacagcgt
    16321 cccgcctgaa aaaaacgcca ggacgtcttg atttccagcg cggcgtgctg caacgcaacg
    16381 ccgacggtga acttgaagtg acgaccaccg gacatcaggg ttcacatata tttagctcct
    16441 ttagtctcgg caactgcttt atcgtgctgg aacgcgatcg cggcaatgtg gacgtgcgcg
    16501 aatgggtgga agtagaaccg tttaacgcgt tgttcggagg cctgtaatgg cggaactcag
    16561 cgatcaggag atgctgcgct acaaccggca aatcattctg cgcggttttg attttgacgg
    16621 tcaggaggcg ctaaaagatt ctcgtgtgct ggtagtcggc ctcggtggac tcggctgcgc
    16681 agcttcgcag tatctggcaa gcgccggtgt cggcaacctg acgctgctcg acttcgacac
    16741 ggtttcgctc tcgaatctgc aacgccagac actgcacagt gatgccacgg tcgggcaacc
    16801 gaaggtggaa tccgcccgtg acgccctgac gcggatcaac ccacatatcg cgattacgcc
    16861 agtcaatgca ctgctagatg acgcagaact tgcagcattg attgctgaac acgatctggt
    16921 gctcgactgt acggataacg ttgcggtacg taatcaactg aacgcaggct gttttgccgc
    16981 gaaggtgccg ctggtttccg gcgcggcaat tcgtatggaa gggcaaatca cggtcttcac
    17041 ttatcaggac ggcgaaccgt gctatcgctg ccttagccgt ttgtttggcg agaacgcgtt
    17101 aacctgcgta gaagcaggcg tgatggcacc gttgataggc gtaattggtt cgttgcaggc
    17161 aatggaagcg atcaaactgc tggcaggtta tggcaaaccg gccagcggga aaatcgtcat
    17221 gtacgatgcg atgacctgtc agtttcgcga aatgaaactg atgcgcaatc cggggtgtga
    17281 ggtgtgcggg cagtaattgc cacctccaag cctgataaga cgcgacaagc gtcgcatcag
    17341 gcatttgctc cagacgccag atgcggcgtg aacgccttat ctggcctacg gttcgggcac
    17401 agatttccaa gcgcggcagg catcggtgca ctccgccacg tcatcagcaa tttatgagga
    17461 tgcagaacgt ccggttaaat cgacgttctg ccaaacgctc cctgccagtc ctgctcaaac
    17521 ttcgtcacag cggcttccac cgccggatag ctaatcatct gttgtgccac atccagtggc
    17581 agagtaattg attcacatcc ggccagtaag cagtccagcg cctgacgcgg ggttttgaaa
    17641 ctcgctgcca gcactttcgc ctgcggcgca tgcattttca ataactggtg taagtcggtc
    17701 acagtctgaa tgccgctacc gccctgagca tcaatacgat taacgtaagg cgcaacatat
    17761 tccgcacctg ccagcgccga cagcagccct tgtgccgcgc catataccgc ggttcccagc
    17821 gtcggaatcc cttccgcttt taacatctta atcgctgcca gcccctcggc tgtcaccgga
    17881 actttcacca cgatatccgc aataatagaa cgcagcttaa gcgcgtcatt caccatccct
    17941 tcagcagtgg tagccattac ctgggcaaac agacgcccct gaccgcccat cgcttcatga
    18001 agttgcggaa gcacaacatc cagcggtttt ttacccgcgg cgataatgct tgggttagtg
    18061 gtcacacccg ccagcggaaa aatacgtgac agcgccttca ccgcaacaac gtctgaagta
    18121 tccagataca gttccataac catcctcaaa atgtactttt tgtctgaatg tagactatca
    18181 cgggaaaact ctctgaacag ttgacgcaca tcaatctaac tttcattcga aagtatttta
    18241 atgtttgaac gaaacagaga ggcaactatg attttcaata ttcagcgcta ctcgacccat
    18301 gatggccccg gtatccgcac ggtcgtattt cttaaaggct gttcgctggg ctgccgctgg
    18361 tgtcagaacc cggaaagccg cgcccgtacg caggatctgc tgtatgacgc acgactgtgt
    18421 ctggaaggct gcgagctgtg cgctaaggcc gcgccggaag tgattgagcg cgcgctgaat
    18481 ggtttgctta ttcatcggga aaagttaacc ccggagcatc tgacggcgtt aaccgactgc
    18541 tgtccgacac aggcgttaac cgtgtgtggt gaagtgaaaa gcgttgagga gatcatggcg
    18601 accgttctgc gcgataaacc gttttacgat cgcagcggcg gcggcttaac gctttcgggt
    18661 ggtgaaccct tcatgcagcc ggaaatggcg acggcgctgc tgcaagcctg ccacgaggca
    18721 ggcattcata ctgcggtaga aacctgtctg catgtgccgt ggaaatatat cgccccttct
    18781 ctgccctata tcgatctgtt tcttgccgat ttaaaacacg ttgccgacgc gccgtttaaa
    18841 cagtggaccg acggtaacgc cgccagggtg ctggataacc tgaaaaaact cgccgcagcg
    18901 ggcaaaaaaa tcattatccg cgtgccgctg attcagggct ttaatgccga cgaaacctct
    18961 gtaaaagcca ttaccgattt tgccgccgac gagctgcacg ttggcgaaat tcattttctg
    19021 ccctaccaca cgctgggcat caacaaatat cacttactta atctgcccta tgacgccccg
    19081 gaaaaaccgc ttgatgcgcc agagctgctc gattttgccc agcagtatgc ctgccagaaa
    19141 gggttaaccg cgaccttacg aggataacaa ccatgaccac actgaaactg gacacgctca
    19201 gcgaccgcat taaagcgcac aaaaatgcgc tggtgcatat tgtgaagccg ccagtctgta
    19261 ccgagcgcgc gcagcactat accgagatgt atcaacaaca tctcgataag ccgatcccgg
    19321 tacgtcgcgc gctggcactg gcgcatcacc tggcgaatcg caccatctgg atcaaacacg
    19381 atgagttgat cattggcaac caggcaagcg aagttcgcgc cgcgccgatc ttcccggaat
    19441 atactgtctc gtggatcgaa aaagagattg atgatctggc agatcgtccc ggtgctggct
    19501 ttgcggtgag cgaagagaac aaacgtgttc tgcatgaagt gtgcccgtgg tggcgcggtc
    19561 agacggtaca ggatcgctgc tatggcatgt ttaccgatga gcaaaaaggt ctgctggcga
    19621 ccggcatcat taaagcggaa ggcaatatga cctccggcga tgcgcacctg gcggtcaatt
    19681 tcccgctgct gctggaaaaa gggcttgatg gtctgcgtga gaaagtagcg gaacgtcgct
    19741 cgcgcatcaa tctgacggtg ctggaagatt tgcacggcga acagttcctg aaagcgattg
    19801 atatcatgct ggtggcagtc agtgaacaca ttgaacgttt cgctgccctg gcgcgtgcaa
    19861 tggccgcgac cgaaacccgc gaaagccgtc gcgatgaact gctggcgatg gcagaaaact
    19921 gcgatcttat cgcccaccag ccgccgcaga ctttctggca ggcgctgcaa ctgtgttact
    19981 tcatccagtt gattttgcag attgaatcta acggtcactc ggtgtcgttt ggtcgtatgg
    20041 atcagtatct ctacccgtac tatcgccgcg acgttgaact gaaccagacg ctggatcgcg
    20101 aacacgccat cgaattgctg catagctgct ggttgaaatt gctggaagtg aacaaaatcc
    20161 gctccggctc ccactcaaaa gcctctgcgg gaagtccgct gtatcagaac gtcaccattg
    20221 gcggacaaaa tctggttgat ggtcaaccaa tggacgcggt gaatccactc tcttacgcga
    20281 tcctcgaatc ctgcggtcgc ctgcgttcca ctcagcctaa cctcagcgtg cgttaccatg
    20341 caggaatgag taacgatttc ctcgacgcct gcgtacatgt gatccgctgc ggcttcggga
    20401 tgccggcatt caacaacgac gaaatcgtga tcccggaatt tattaaactc ggtattgaac
    20461 cgcaggacgc ttatgactac gcagcgattg gttgtataga aaccgccgtc ggtggcaaat
    20521 ggggctatcg ctgtaccggc atgagcttta tcaacttcgc ccgcgtgatg ctggcggcgc
    20581 tggaaggcgg gcgtgatgcc accagcggca aagtgttcct gccacaagaa aaagcgttgt
    20641 cggcaggtaa cttcaacaac ttcgatgaag tgatggacgc gtgggatacg caaatccgtt
    20701 actacacgcg taaatcaatc gaaatcgaat atgtcgtcga caccatgctg gaagagaacg
    20761 tgcacgatat tctctgctcg gcgctggtgg atgactgtat tgagcgagcg aaaagtatca
    20821 agcaaggcgg cgcgaaatat gactgggttt ctggcctgca ggtcggtatt gccaacctcg
    20881 gcaacagcct ggcggcagtg aagaaactgg tgtttgaaca gggcgcgatt ggtcagcaac
    20941 agctggctgc cgcactggcg gatgacttcg atggcctgac tcatgagcaa ctgcgtcagc
    21001 ggctgattaa cggtgcgccg aagtacggca acgacgatga taccgtcgat acgctgctgg
    21061 ctcgcgctta tcagacctat atcgacgaac tgaaacagta ccataaccca cgctacggtc
    21121 gtggtccggt tggcggcaac tattacgcgg gtacgtcgtc tatctccgct aacgtaccgt
    21181 ttggcgcgca aactatggca acgccggacg ggcgtaaagc ccacaccccg ctggcagaag
    21241 gcgctagtcc ggcctctggt actgaccatc tcggccctac tgcggtcatt ggctcagtgg
    21301 gtaaactgcc tacggcagcg attctcggcg gcgtgttgct caaccagaaa ctgaatccgg
    21361 caacgctgga gaacgaatct gacaagcaga aactgatgat cctgctgcgt accttctttg
    21421 aagtgcataa aggctggcat attcagtaca acatcgtttc ccgcgaaacg ctgctggagg
    21481 cgaaaaaaca tccggatcag taccgcgatc tggtagtgcg tgtcgcgggt tattccgcct
    21541 tcttcaccgc gctctctcca gacgctcagg acgatatcat cgcccgtact gaacatatgc
    21601 tgtaatctct tcggcccggc tgcggcgtcg ccgggctaaa tcattcacat catcaatttc
    21661 atccttactt tcattcgaaa tataatttgt gctctgcgtc acattgttat tacactgtta
    21721 cggtttcatt ttcgagccag gagcacacct atgagcgtaa aagttatcgt cacagatatg
    21781 gacggtactt ttcttaacga cgccaaaacg tacaaccaac cacgttttat ggcgcaatat
    21841 caggaactga aaaagcgcgg cattaagttc gttgtcgcca gcggtaatca gtattaccag
    21901 cttatttcgt tctttcctga gctaaaggat gagatctctt ttgtcgcgga aaacggcgca
    21961 ctggtttacg aacatggcaa gcaactgttc cacggcgaac tgacccgaca tgaatcgcgg
    22021 attgttattg gcgagttgct aaaagataag caactcaatt ttgtcgcctg cggtctgcaa
    22081 agtgcatatg tcagcgaaaa tgcccccgaa gcatttgtcg cactgatggc aaaacactac
    22141 catcgcctga aacctgtaaa agattatcag gagattgacg acgtactgtt caagttttcg
    22201 ctcaacctgc cggatgaaca aatcccgtta gtgatcgaca aactacacat agcgctggat
    22261 ggcattatga aacccgtcac cagtggtttt ggctttatcg acctgattat tcccggtcta
    22321 cataaagcaa acggtatttc gcggttactg aaacgctggg atctgtcacc gcaaaatgtg
    22381 gtagcgattg gcgacagcgg taacgatgcg gagatgctga aaatggcgcg ttattccttt
    22441 gcgatgggca atgctgcgga aaacattaaa caaatcgccc gttacgctac cgatgataat
    22501 aatcatgaag gcgcgctgaa tgtgattcag gctgtgctgg ataacacatc cccttttaac
    22561 agctgacctc tctaccggag ccatgcgctc cggttttccc ttctttttac atcctgtaca
    22621 tcacatcaaa tcattaaact tgcattaact tatttataac ttgtattgtc acatgcaaat
    22681 tagcatagtt tcgaatgaaa gttaatgcaa ggtggttatg gcttctactt ttaccagcga
    22741 cacattgcct gccgatcaca aagcagctat ccgtcagatg aagcacgcgc tgcgggcgca
    22801 gcttggcgat gtccagcaga tctttaatca gctaagcgat gacattgcca cgcgagtggc
    22861 tgaaatcaac gcactcaaag cacagggcga tgccgtctgg ccggtgctgt cttatgccga
    22921 tatcaaagca ggtcatgtta ctgcagagca gcgcgaacag attaaacgtc gcggttgtgc
    22981 ggtgataaaa ggccatttcc cccgcgaaca agcgctaggc tgggatcagt cgatgctgga
    23041 ctatctgaac cgcaaccgct ttgacgaggt ctacaaaggc cccggcgata atttcttcgg
    23101 gacgctcagc gcttcacgtc ccgagattta ccccatctac tggtcgcagg cgcaaatgca
    23161 ggcccgccag agtgaagaaa tggcgaatgc gcagtcgttt ctcaatcgtc tgtggacatt
    23221 tgaaagtgat ggaaagcaat ggtttaaccc ggatgtgagc gtcatctacc ctgaccgtat
    23281 ccgccgcctt ccgcccggaa cgacctccaa aggtcttgga gcgcataccg actccggggc
    23341 gctggaacgc tggctgcttc cagcgtatca gcacgttttc gccaacgtct ttaatggcaa
    23401 tctggcgaaa tacgatccct ggcatgcggc acatcgtacg gaagttgaag agtacacggt
    23461 ggacaacacc accaaatgtt ccgtgtttcg gacattccag ggctggactg cgctctcgga
    23521 tatgctgcct ggtcaggggt tgctgcacgt cgtgcccatt cctgaagcca tggcgtacgt
    23581 actgttacgt ccgctgcttg atgatgtgcc ggaggatgaa ctgtgcggcg tagcgcccgg
    23641 aagagtgttg ccggtatcag agcaatggca tccactgtta attgaggcgt taaccagcat
    23701 tccaaaactc gaggccggag actccgtctg gtggcactgc gacgtcatcc attccgttgc
    23761 ccccgttgaa aatcaacagg gttggggcaa cgtgatgtac attcctgcgg caccgatgtg
    23821 cgagaaaaat cttgcctacg cgcacaaggt gaaggccgca ctggaaaaag gcgcatcgcc
    23881 gggcgacttc ccgcgcgagg actatgaaac aaactgggaa ggacgcttta cgcttgccga
    23941 cctcaacatt cacggtaagc gagcgttggg catggatgtt tgagagttaa tggaagtgaa
    24001 ttgatgtggg attaagaaaa tcgattcgac gttactcaga ttgctgacaa gtgcgctttc
    24061 ttaatgcctg atgcggcgtt aacgccttat ccggcctaca aagtcctgca gattcaatat
    24121 attacaggta ctgcgtaggc ctgataagcg tagcgcatca ggctattttg catttgtcat
    24181 cagccgtcgt caccccgaca gtatcggggt gacggataat cacttactcg atccctttac
    24241 tacgcaggta atcttcgtaa ttaccgctaa agtcgatcac gcgttccggg gtgatttcca
    24301 gaatgcgggt cgccagggag cttacgaact cacggtcgtg tgaaacaaag atcagcgtgc
    24361 cctgatacag ttccagcgcc atgttcagtg actcgatgga ttccatatcc aggtggttgg
    24421 tcggttcgtc catgatcaga atgttcggct tctgcatcat taacttacca aacagcatcc
    24481 gccctttttc cccaccggaa agcactttag ctggcttttt gatgtcgtcc tggctgaaca
    24541 gcaaacgacc gagaatgctg cgtaccgcct gctcgtcatc gccttcctgc ttccactgac
    24601 tcatccattc gaacacggtc agatcatttt caaactcata ttcgtggtcc tgggcgtagt
    24661 aaccaatgcg cgcgttttca gaccatttta cggtgccgct gtccggttgc agatcgccca
    24721 ccagcgtttt cagcagcgtt gatttaccga cgccgttggt acccagtacc gcaagttttt
    24781 caccgacctc cagcagcagg ttgagatttt taaacagtgg accgttatca aaccctttgg
    24841 tcagaccttc cacttccagc gcgttacgga acagtttctt atcctgttca aaacggataa
    24901 acgggttctg acggctggag gctttcacct cttccagttt gattttatca atctggcgcg
    24961 cgcgcgaagt tgcctggcga gatttcgagg cgttggcgct aaagcggcta acgaaagatt
    25021 gcaactcagc aatctgcgct ttcttcttgg cgttatcggc cagcagacgt tcacgcgctt
    25081 gggtcgccgc cgtcatgtac tcatcgtagt tacccggata aacgcgcagc tcgccgtaat
    25141 ccagatccgc catgtgggta cagaccatgt taaggaagtg acggtcgtgc gagatgatga
    25201 tcatggtgct gtcacgctcg ttcagcacct gttccagcca gcgaatggtg tcgatgtcga
    25261 ggttgttggt cggttcgtcg agcaggagaa tatccggctc agcaaacagc gcctgcgcca
    25321 gaagcacacg cagcttccag ccaggagcaa cttcactcat cgggccgtag tgctgttcca
    25381 ctggaattcc cacgccaagc aacagttcac cggcgcgagc ttccgcagag taaccgtcca
    25441 tttcgccgta tttcacttcc agatcggcca ctttatagcc gtcttcttca ctcatttccg
    25501 gcaaagcata gatgcggtcg cgctcctgct tcacttccca caactcttta tgccccatga
    25561 tcaccgtatc cagcacagtg aactcttcaa aggcaaactg atcctgacgc agcttaccaa
    25621 tgcgctcgtt gggatcgagg gaaacgttac ccagcgtcgg ctcaaggtcg ccgccgagaa
    25681 tcttcataaa ggtggattta ccactaccgt tcgcgccaat caggccgtaa cggttgccgc
    25741 cgccgaattt gacggaaatg ttttcaaaca acggcttact gccgaactgc atggtgacgt
    25801 tactggaaac taacacaggc gtatcctgaa aagagatatg acaaaccgcg cattatgcca
    25861 taagtctgaa agaagatcct gctcctcgct taaggtctac actcaagcca aaggcaaaaa
    25921 atgtgatttc gtacacatct gatttcactg tgagctggaa tgaacttata atgcgcttcc
    25981 aatactctaa tattctcaac ccaatggcct gccaggcaca aaatctcgct taacatgaat
    26041 atgaaattga aaacattatt cgcagcggcc ttcgctgttg tcggcttttg cagtaccgcc
    26101 tctgcggtaa cttatcctct gccaaccgac gggagtcgcc tggttggtca gaatcaggtg
    26161 atcaccattc ctgaaggtaa cactcagccg ctggagtatt ttgccgcgga gtaccagatg
    26221 gggctttcca atatgatgga agcgaacccg ggtgtggata ccttcctgcc gaaaggcggt
    26281 actgtactga acattccgca gcagctgatc ctgccggata ccgttcatga aggcatcgtc
    26341 attaacagtg cagagatgcg tctgtattac tatccgaaag ggaccaacac cgttatcgtg
    26401 ctgccgatcg gcattggtca gttaggcaaa gatacgccta tcaactggac caccaaagtt
    26461 gagcataaga aagcaggccc gacctggacg ccgaccgcca aaatgcacgc agagtaccgc
    26521 gctgcgggcg aaccgctacc ggctgtcgtt ccggcaggtc cggataaccc gatgggtctg
    26581 tatgcactct acatcggtcg cctgtatgct atccatggca ccaacgccaa cttcggtatc
    26641 ggcctgcgtg taagtcatgg ttgtgtgcgt ctgcgtaacg aagacatcaa attcctgttc
    26701 gagaaagtac cggtcggtac ccgcgtacag tttattgatg agccggtaaa agcgaccacc
    26761 gagccagacg gcagccgtta tattgaagtc cataacccgc tgtctaccac cgaagcccag
    26821 tttgaaggtc aggaaatcgt gccaattacc ctgacgaaga gcgtgcagac agtgaccggt
    26881 cagccagatg ttgaccaggt tgttcttgat gaagcgatca aaaatcgttc cgggatgccg
    26941 gttcgcctga attaatcgtc aaaacgtaaa acaaaggcgg actcataatc cgcctttttt
    27001 atttgccaga ccttaccagg ccgggatcat aactaacaaa acgtatccca ccagcgccgc
    27061 ccacaacagc atcggtattg aatagagatg gaaacgccac cagatgcggc gatcgttcgc
    27121 catacgtagc gcaatcaaat ttgccagcga tcctggtaat aaaccaaagc cccccacgtt
    27181 taccgcccat gccagtaata aagacggagg tacataattg agcagcaaaa tggtactcgg
    27241 cacgttactg atcacctgcg ataaaccgac tgccgttaac cataacccgg gttcagataa
    27301 atgactcacg ttacccaaca cgccttgcaa cgctggaagc tgggtcagta aatggacgtc
    27361 gataaacatc gccataaaca ccagcagcaa cgtccagtcc acactcaaca ccacgcgacg
    27421 cgccagcagc acaaagcccg ccgccacaat caccagtccc cacagctctt gtttgaactc
    27481 cagcgccgtc agaaagacga tatacagccc cagacaattc cacaccagcc gcggtttcca
    27541 ctccggtgtt tgcacccccg tatggtattg cagcgccttt ccagggaaac aacaccagca
    27601 caacagcagg agtgtcagca tcattgcgcc agccatcggt gccatttggg caataaatcc
    27661 ggcaaacgaa agaccagaac gtccccagat aagaatattt tgcgggttgc caattggcgt
    27721 cagtagcgaa ccagcgttga ctgccagcgc ctcaaaaata atcagccgat taaccgggat
    27781 ctcacacagt ctttttagcg tgatagtcag cggaacaaca ataaacagcg cgacatcgtt
    27841 ggtcagaaag gtagaaagca gcgccgccgc cagcaccata aacatcgcca gccgacgctc
    27901 cgtagcaaag cggcgcacca ttttgcgccc cagcacatca aaataaccgc ttaactccac
    27961 acctttggtc agcagcatca ggccgcttaa ggtgatgatg gtgtgccagt cgatagcagc
    28021 aggccaggat ttcggtgcaa agggcacgaa aaagcttaat ccgataccaa caagaattaa
    28081 taactgaaaa aaacgatcgc cttgcagcgt gcgtaaaaaa ggcaggctca tttggcaccg
    28141 tgtttctggg taaacaaacg aaaagcgtcg agcgtctctt cactaacatg gtgctccatg
    28201 ccttccgcgt cgcgacgggc gatttccgga ctgacgccca acaccaacaa gaaattttcg
    28261 actatctgat gtcgctcgcg gctttcctgc gccagcttct ctccttctgc cgttaaaaac
    28321 acgccacgcc aggggatcat ttcaatcagc cccatggttg ccagccgctt aagcatttta
    28381 gccaccgtcg gttgcgaaac tcccagacga gcagccatgt ccacctgacg agcttccccc
    28441 acttccctga tcaagtcaga aatcagctca acgtaatcat caataagctc gcgccgatgc
    28501 gcctctctga cctggcggaa cccttcaacg tgctcttcca cgttcactaa ctgcgtcact
    28561 ttttttgcta ttggcgtacc tgcgcgacga ctcattgtgc ttcctcatta tggtgacgca
    28621 tacatagcgt tctcatttcg tgcgcacatt gtaaaccaga gttgcgaagg tacaaaaaat
    28681 taacgtttta gcaatagcta tataatatag cctgtgctat atctgtatgt aatgcaatca
    28741 tccctcaagg atcgacggga ttagcaagtc aggaggtctt atgaatgagt tcaagaggtg
    28801 tatgcgcgtg tttagtcatt ctccctttaa agtacggtta atgctgctct ctatgttgtg
    28861 cgatatggtc aacaacaaac cgcagcaaga taaaccttca gataaatagc ggcgtcgcgg
    28921 tacgccgctt cactcctgct tttatgcagg cataacgcgt tttggtctga aaaaaaccac
    28981 tttttgtcgg atttgcaatc cccttcgcaa aagatttgtt cgtcagtagt tgacctgaac
    29041 ggcggctcgc tctatcttct tgcagccctg cgtatattgc ggctcgcgga tgcggacccc
    29101 tttcccctct tcacgcactc ttgcaggtat tgacccttga cgccagggta agcacatggc
    29161 gtttgttacg atagtggcat atgaatttaa ccctcaaaga atcgcttgtt acccgtagcc
    29221 gggtatttag cccgtggact gcgttctact ttttacagtc gctattaatt aacctcggct
    29281 taggttaccc cttcagtttg ctctacaccg ctgcgtttac ggctattttg cttttgctat
    29341 ggcgaacatt gcctcgcgta caaaaagttc tggtcggtgt cagttcgctg gtggcggctt
    29401 gttatttccc ttttgctcag gcctacggcg cgcctaattt caatacattg ctggcattgc
    29461 actccaccaa tatggaagag tcgaccgaaa tcctgacgat ttttccgtgg tacagctacc
    29521 tggtcggctt atttattttt gcgctcggcg taatagcaat caggcgaaaa aaagagaatg
    29581 aaaaagcgcg ctggaatacc ttcgacagcc tgtgtctggt attcagtgtg gcgacatttt
    29641 ttgttgctcc cgtgcaaaac ctggcctggg gtggcgtatt taaactgaaa gatactggct
    29701 atccggtatt tcgttttgct aaggatgtca tcgtcaataa taacgaggtg attgaagagc
    29761 aagaacggat ggcaaaactt tccggaatga aagatacctg gacggtcact gccgttaagc
    29821 cgaagtatca gacctatgtg gtagtgatcg gtgaaagcgc gcgtcgcgat gccctcggtg
    29881 cctttggcgg tcactgggac aataccccgt ttgccagcag cgttaacggt ttgatatttg
    29941 ctgactacat tgctgccagt ggctccacgc agaaatcgct tggcttaacg ctcaatcgcg
    30001 tagtcgatgg caaaccacag tttcaggata actttgtcac cctggcaaat cgcgcgggct
    30061 tccagacctg gtggttttcc aaccagggcc aaatcggcga atacgatact gctatcgcca
    30121 gcatcgccaa acgtgcagat gaagtgtact tcctgaaaga aggtaatttt gaagcagata
    30181 aaaacacgaa agacgaagcg ttactggata tgaccgctca agtgctggcg caagagcact
    30241 cgcaaccgca gctgattgtt ctgcatctga tgggctcgca tccgcaggcc tgcgacagga
    30301 cacaaggcaa atacgaaacc tttgtgcaat cgaaagaaac gtcgtgctat ctctatacca
    30361 tgacgcaaac ggacgattta ctgcgcaagc tgtacgatca gttacgcaac agcggcagca
    30421 gcttctctct ggtttacttt tctgaccacg gtctggcctt taaagagcgc ggcaaagacg
    30481 tgcaatacct tgcccatgat gataaatatc agcaaaattt ccaggtgcct tttatggtca
    30541 tttccagcgg cgataaagcg catcgggtga ttaaagcccg ccgctcagcc aatgacttct
    30601 taggcttttt ctcgcagtgg acgggaatta aagcgaagga aataaatatc aaatacccgt
    30661 ttatatctga gaagaaagcc gggccgatat acatcaccaa cttccagtta cagaaggtgg
    30721 attacaacca tctcggaacc gatattttcg acccgaaacc ttaaaaacaa aaatccgccc
    30781 cgagaggcgg atttttatat caccaaagtg attagaagcg gtaaccaaca ccggcaatcc
    30841 aggtgcctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc agagcaacgt
    30901 tttccatcgg gttgaactgc agaccagcac cgtaggagaa accgtagtcg ctggtgtcgt
    30961 gtttgtaggt cgggtattca gtggtctgga atttaccata acccacaccc actacaccgt
    31021 agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg tagtactggt
    31081 ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg aaagaaccga
    31141 tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa ccgcccattt
    31201 tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacggta gaagtcgcag
    31261 ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga catgcaattt
    31321 ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata tatcaaaaat
    31381 taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta acagaacgtt
    31441 tccatacacc gctatccatc taaatttaaa tcactttttc agagaactgc gtaagtatta
    31501 cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa tgattccatc
    31561 aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc tcaaccagca
    31621 ttgggtatat ccagtacact ccacgcttta cttaagtcta gatatttgtg ggagaaagga
    31681 tgcctggttc attacgtaaa atgccggtct ggttaccaat agtcatattg ctcgttgcca
    31741 tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg gtgagcgcac
    31801 cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg ttctttaagc
    31861 catggcgact gcgctttgcc aaagagcaac ggttaccgct gttgttttac ggcgtttcgc
    31921 tgggtgggat gaattatctt ttttatcttt ctattcagac agtaccgctg ggtattgcgg
    31981 tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt cgcccggtag
    32041 atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt cctgctaccg ctggggcaag
    32101 acgtttccca tgtcgattta accggctgtg cgctggcact gggggccggg gcttgttggg
    32161 ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg acggtagcaa
    32221 ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag gctggtgaag
    32281 cactctggca ctggtcggtt attccattgg gtctggctgt cgttattctc tcgaccgctc
    32341 tgccttattc gctggaaatg attgccctca cccgtttgcc aacacggaca tttggtacgc
    32401 tgatgagcat ggaaccggcg ctggctgccg tttccgggat gattttcctc ggagaaacac
    32461 tgacacccat acagctgctg gcgctcggcg ctatcatcgc cgcttcaatg gggtctacgc
    32521 tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt tacatttctg
    32581 tatggttatg cataaccatg cagaatttct cgctactttt cctctacacc gtatttatat
    32641 atcgaattat gcaaaagcat atttattccg aaaattcctg gcgagcagat aaataagaat
    32701 tgttcttatc aatatatcta actcattgaa tctttattag ttttgttttt catgcttgtt
    32761 accactatta gtgtgatagg aacagccaga atagcggaac acatagccgg tgctatactt
    32821 agtctcgtta attactggga cataacatca agaggatatg aaattatgag taccgctaaa
    32881 ttagttaaat caaaagcgac caatctgctt tatacccgca acgatgtctc cgacagcgag
    32941 aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc agtttattga tctttctttg
    33001 attaccaaac aagcgcactg gaacatgcgc ggcgctaact tcattgccgt acatgaaatg
    33061 ctggatggct tccgcaccgc actgatcgat catctggata ccatggcaga acgtgccgtg
    33121 cagctgggcg gtgtagctct ggggaccact caagttatca acagcaaaac cccgctgaaa
    33181 agttacccgc tggacatcca caacgttcag gatcacctga aagaactggc tgaccgttac
    33241 gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag cgaaagatga cgacaccgca
    33301 gatatcctga ccgccgcgtc tcgcgacctg gataaattcc tgtggtttat cgagtctaac
    33361 atcgaataaa tccatcgctg atggtgcaga actttagtac ccgataaaag cggcttcctg
    33421 acaggaggcc gttttgtttt gcagcccacc tcaacgcact tatttagtgc atccatctgc
    33481 tatccccagc tgattaagta aattttttgt atccacatca tcacacaatc gttacataaa
    33541 gattgttttt tcatcaggtt ttacgctaaa taatcactgt gttgagtgca caattttagc
    33601 gcaccagatt ggtgccccag aatggtgcat cttcagggta ttgccctata aatcgtgcat
    33661 cacgtttttg ccgcatctcg aaaaatcaat gagttgcaaa attggcacga ttttttcata
    33721 tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa aaaggaaatg ctatgaagtc
    33781 tgtattaaaa gtttcactgg ctgcactgac cctggctttt gcggtttctt ctcatgccgc
    33841 ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt ccgtttgaat ttaaacaggg
    33901 cgataaatat gtgggctttg acgttgatct gtgggctgcc atcgctaaag agctgaagct
    33961 ggattacgaa ctgaagccga tggatttcag tgggatcatt ccggcactgc aaaccaaaaa
    34021 cgtcgatctg gcgctggcgg gcattaccat caccgacgag cgtaaaaaag cgatcgattt
    34081 ctctgacggc tactacaaaa gcggcctgtt agtgatggtg aaagctaaca ataacgatgt
    34141 gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg aagagcggta ctggctccgt
    34201 tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt cagttcccga acatcgataa
    34261 cgcctatatg gaactgggca ccaaccgcgc agacgccgtt ctgcacgata cgccaaacat
    34321 tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa gcggtaggtg actctctgga
    34381 agcgcagcaa tacggtattg cgttcccgaa aggtagcgac gagctgcgtg acaaagtcaa
    34441 cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac gaaatctaca aaaaatggtt
    34501 cagtactgaa ccgaaataat aacgctacac ctgtaaagcg cactggcagt tccctctccc
    34561 ccctggggag aggattaggg tgaggggcgc aaacccgctc cggggccatt aattaccctg
    34621 aatttgatta tttacaccac ggtaacagga acaacatatg cagtttgact ggagtgccat
    34681 ctggcctgcc attccgcttc tgattgaagg tgccaaaatg accctgtgga tttcggtcct
    34741 cggtctggct ggcggtctgg taatcggatt gctggcaggt tttgcacgca ccttcggtgg
    34801 ttggatagcc aaccacgtcg cgctggtctt tattgaagtg atccgcggca cacctatcgt
    34861 cgtccaggtg atgtttatct atttcgccct gccgatggcg tttaacgact tacgcatcga
    34921 cccatttact gcggcggtgg tcaccatcat gatcaactcc ggcgcgtaca ttgcggaaat
    34981 cacgcgtggt gcggtgctgt ctatccacaa aggttttcgt gaagcaggac tggcgctcgg
    35041 tctttcacgt tgggaaacca ttcgctacgt cattttaccg ctggcactgc gtcgtatgct
    35101 gccgccgctg ggtaaccagt ggatcatcag cattaaagac acctcgctgt ttattgttat
    35161 cggcgtggcg gaactgaccc gtcaggggca agaaattatt gccggtaact tccgcgccct
    35221 tgagatctgg agcgccgtgg cggtgttcta tctgattatt accctggtgc tgagctttat
    35281 tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg aatttaaaaa cgtctccaag
    35341 cactttggcc caacccaggt gctgcacaat atcgatttga acattgccca gggcgaagtc
    35401 gtggtgatta tcgggccgtc cggttccggt aaatcgaccc tgctgcgctg catcaacaaa
    35461 ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc tgaaggtcaa cgatccgaaa
    35521 gttgacgagc gcctgattcg ccaggaagca ggtatggtgt tccagcagtt ttacctcttc
    35581 ccgcatctga cggcgctggg aaacgtcatg tttggtccgc tacgcgtgcg tggcgcgaac
    35641 aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga aagtcggtct ggcagaacgt
    35701 gcacatcact acccttccga actttctggt ggtcaacagc agcgtgtggc gattgcccgc
    35761 gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac cgacttccgc tcttgacccg
    35821 gaactgcgcc atgaagtgct gaaggttatg caggatctgg ctgaagaagg gatgacgatg
    35881 gtgatcgtga cccacgaaat cggttttgcc gagaaagtag cttcgcggct gatctttatc
    35941 gacaaaggcc ggattgcgga agatggcaat ccgcaggtgt tgatcaagaa cccgccgagc
    36001 cagcgcttgc aggaattttt gcagcacgtc tcttaataag acacattgcc tgatgcgcta
    36061 cgcttatcag gcctacagga tatctggcaa cttattaaaa ttgcatgaac ttgtaggacg
    36121 gataaggcgt tcacgccgca tccggcaaaa aagcccgcac gttgtcagca acctgcttaa
    36181 tatcccttcc tccctttcac ccgaaaggga ggcacaccag attcctctca tttaaaatcg
    36241 cccctccccc agcatctata cttatctttt tgctctgttt tctcactgga ggagtcatgc
    36301 ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg gtatccatac
    36361 ccggcgttac aaccacaacg acaacggact caacgactga accggccccg gaaccggata
    36421 tcgaacaaaa aaagcggcct atggcgcact ggcggatgtg ctggataacg acacctcgcg
    36481 taaagagttg atcgaccagt tgcgtactgt cgccgcgacg ccccctgcgg aaccggtccc
    36541 gaagatcgtg ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt
    36601 cagccgccat tatggtgaag ccctttccgc ccgcttcggg caactttatc gcaatatcac
    36661 cggctccccg cataagccat ttaatccaca aaccttcagc aatgcgctga gccatttttc
    36721 aatgttagcg gtattagtgt ttggttttta ctggctgatt cgcctgtgcg cactgccgct
    36781 atatcgcaaa atgggtcagt gggcgaggca aaaaaatcgt gagcgcagca actggttgca
    36841 gcttccggcg atgattattg gggcgtttat tatcgacctg ctgttactgg ccctgacatt
    36901 atttgtcggc caggtattaa gcgacaacct gaatgcaggc agtcgcacca tcgctttcca
    36961 acaaagtttg tttctcaacg cctttgccct gattgaattt ttcaaagccg tactacgcct
    37021 gattttttgc ccaaacgtgg cggagctgcg cccgttcacg attcaggacg agagcgcccg
    37081 ttactggggt cgtcgcctga gctggttaag cagcctgatt ggctatggcc tgattgtggc
    37141 cgtgccgatt atctctaatc aggtgaatgt acagataggt gcgctggcga acgtcatcat
    37201 tatgctgtgc atgaccgtct gggcgttgta cctgatcttc cgaaataaaa aagagattac
    37261 ccagcatatg ctcaactttg cggagcattc gctggctttt ttcagcctgt ttatccgcgc
    37321 ctttgcgctg gtgtggcact ggctggcaag cgcctatttt atcatgctgt ttttcttttc
    37381 gttgttcgat ccgggcaaca gtctgaaatt tatgatgggt gcaacggtgc gcagcctggc
    37441 gattattggt atcgcagcgt ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat
    37501 caccctctcg ccacatactc agcgtaacta tccggagcta caaaaacggc tgaatggctg
    37561 gctttcggcg gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcag taatgttgct
    37621 gttgagcgca tggggattgt tcgatttctg gaactggctg caaaacggcg cggggcagaa
    37681 aaccgtagat atcctgatcc gcatcgcact cattcttttc ttctcggcgg ttggctggac
    37741 ggtgctcgcc agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccgctacc
    37801 cagcgcccgt acgcgtaccc tgctgacgct gttccgtaac gcgctggcgg tgattatcag
    37861 taccatcacc atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc
    37921 aggtgccggg gcattaggtc tggctatctc gtttggttcg caaacgctgg taaaagatat
    37981 tatcaccggg gtatttattc agtttgaaaa cggcatgaac actggagatt tggtgactat
    38041 cgggccgttg accggcacag tggaacggat gtcgattcgc tccgtgggcg tgcgccagga
    38101 taccggggcg tatcacatca ttccgtggtc ttcgataacc acctttgcta acttcgtccg
    38161 cggtattggt tcggtagtgg caaactacga tgttgatcgc catgaagatg ctgataaagc
    38221 caatcaggca ctgaaagatg cggtaacgga attgatggaa aacgaagaaa ttcgcgggct
    38281 gattattggt gaaccgaact ttgccgggat tgtcggctta agcaataccg cgtttacact
    38341 gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg gtacgctttg ccctcgacag
    38401 ccaggtgaaa aaacatttcg acctggcggg cgttcgcgcg ccagtgcaga cttatcaggt
    38461 gctgcctgct ccgggcgcga ccccggctga accgctgccg ccgggggagc caacgcttta
    38521 acgttggcga ttaacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa
    38581 gcgactctgc ttttgcccct gggccatctc tttttttacc accttcaccg cgcccacgtc
    38641 ggtcagagca cgatacaacg gcggtaagtt ttcaccacga gataccagcg aggtaaacca
    38701 catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa agttgacttc
    38761 accgccttca caccacaact cctgttgctg accaccaaag ttcagtgcat cgtctttgtt
    38821 cagccccagg ttacggcgtt tacgctcact acctgcccgc gccgcagcgg cggaatcgtg
    38881 gaatggcggg ttacacaagg tcgcgtcgta ttgctcgttt ttgtggatga tgccgttaaa
    38941 aatcgcccca ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattagc
    39001 actgataatc gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg
    39061 ccagccatat tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa cgccgatatc
    39121 cagaatgctg gcattcgctg gaattgttcc gcttgcttca gccagtaaat cggcaaggtg
    39181 atgaatataa tccgcccggc ccggtactgg tgggcagaga aaaccgtcgg ggatatccca
    39241 gttcgctacg gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg
    39301 attggcaaag tctacgcttt gctcccctgc gggtgtaagc gtgaggaatt gcctgagttc
    39361 aggattgacc tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg
    39421 caaccccggt ttctgggcgg acatagcatt ctcctttaag cagcggcgta agatacccgc
    39481 ataacgcgac agggtaaata tctggctgcg tttatcattc cccaaaacct taacatggct
    39541 atggtttatg gcaattcatc ataaaaatca gcattatgca tgtcattgat ttgtcttatt
    39601 ttgtaaaaat atatcgttca aaaaataagc atttctctgt caattttttg cgtcatttgc
    39661 atcgcgcagg cgacctacac ttatcaggca ctacccgttg tatttatgag gatgtcatta
    39721 tgaaaaagtg cctcacacta ctgattgcca ccgtcctgag cggaatctct ctcacggctt
    39781 atgccgcgca accgatgagt aacctggaca gcggtcagct acggcccgcc ggtaccgttt
    39841 cggcaactgg cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg
    39901 aacaaggcgc gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttaggta
    39961 ctgcaaccat ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt
    40021 cgcctgtttt gtaatcaaaa aacatcaaat atgaacgcaa tgtaaataaa tgtatttctt
    40081 tttcgcgcaa tgggtgatag aaaatcgcat caagtgataa tgcttatcaa aattattatc
    40141 acttgcacga acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc
    40201 gtggcagtga aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca
    40261 atttccctgc cagacaattt cattcgctca cgttctttgc cggtctttgt attggtatta
    40321 cgcctgtggc acaggcactc gccgccgaag ggcaagctaa cgcggatgac
//
LOCUS       DAJVQR010000028        39990 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVQR010000028 DAJVQR010000000
VERSION     DAJVQR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..155
                     /locus_tag="OQM52_001558"
     CDS             <1..155
                     /locus_tag="OQM52_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1563455.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OQM52_001559"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OQM52_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1563456.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OQM52_001560"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OQM52_001560"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1563457.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OQM52_001561"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OQM52_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OQM52_001562"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OQM52_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1563458.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OQM52_001563"
     CDS             complement(5626..7134)
                     /locus_tag="OQM52_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1563459.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OQM52_001564"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OQM52_001564"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1563460.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OQM52_001565"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OQM52_001565"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1563461.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OQM52_001566"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OQM52_001566"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1563462.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OQM52_001567"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OQM52_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1563463.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OQM52_001568"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OQM52_001568"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1563464.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OQM52_001569"
     CDS             complement(14283..15602)
                     /locus_tag="OQM52_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCS1563465.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OQM52_001570"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OQM52_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1563466.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OQM52_001571"
     CDS             16699..17034
                     /locus_tag="OQM52_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1563467.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OQM52_001572"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OQM52_001572"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1563468.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OQM52_001573"
     CDS             complement(17790..19172)
                     /locus_tag="OQM52_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1563469.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OQM52_001574"
     CDS             complement(19358..20302)
                     /locus_tag="OQM52_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1563470.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OQM52_001575"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OQM52_001575"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1563471.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OQM52_001576"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OQM52_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1563472.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OQM52_001577"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OQM52_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1563473.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OQM52_001578"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OQM52_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1563474.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OQM52_001579"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OQM52_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1563475.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OQM52_001580"
     CDS             complement(25947..26768)
                     /locus_tag="OQM52_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1563476.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OQM52_001581"
     CDS             complement(26868..26951)
                     /locus_tag="OQM52_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563477.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OQM52_001582"
     CDS             complement(27044..27379)
                     /locus_tag="OQM52_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1563478.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OQM52_001583"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OQM52_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30047
                     /locus_tag="OQM52_001584"
     CDS             29136..30047
                     /locus_tag="OQM52_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1563479.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30182..31402
                     /gene="mlc"
                     /locus_tag="OQM52_001585"
                     /gene_synonym="dgsA"
     CDS             30182..31402
                     /gene="mlc"
                     /locus_tag="OQM52_001585"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1563480.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31527..32222
                     /gene="bioD"
                     /locus_tag="OQM52_001586"
     CDS             31527..32222
                     /gene="bioD"
                     /locus_tag="OQM52_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1563481.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OQM52_001587"
     CDS             complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OQM52_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1563482.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OQM52_001588"
     CDS             complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OQM52_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1563483.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34283..35137)
                     /locus_tag="OQM52_001589"
     CDS             complement(34283..35137)
                     /locus_tag="OQM52_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1563484.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OQM52_001590"
     CDS             complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OQM52_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1563485.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35767..37805)
                     /locus_tag="OQM52_001591"
                     /pseudo
     CDS             complement(35767..37805)
                     /locus_tag="OQM52_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37866..38975)
                     /locus_tag="OQM52_001592"
     CDS             complement(37866..38975)
                     /locus_tag="OQM52_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1563486.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38958..>39990
                     /locus_tag="OQM52_001593"
     CDS             38958..>39990
                     /locus_tag="OQM52_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1563487.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10163 a         9700 c        10243 g         9884 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtgaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
    29761 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
    29821 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
    29881 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
    29941 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
    30001 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
    30061 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
    30121 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
    30181 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
    30241 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
    30301 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
    30361 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
    30421 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
    30481 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
    30541 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
    30601 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
    30661 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
    30721 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
    30781 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
    30841 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
    30901 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
    30961 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
    31021 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
    31081 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
    31141 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
    31201 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
    31261 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
    31321 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
    31381 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
    31441 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
    31501 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
    31561 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
    31621 aaagggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
    31681 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
    31741 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
    31801 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
    31861 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
    31921 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
    31981 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
    32041 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
    32101 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
    32161 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
    32221 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
    32281 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
    32341 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
    32401 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
    32461 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
    32521 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
    32581 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
    32641 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
    32701 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
    32761 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
    32821 tcgctatgat taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg
    32881 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
    32941 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
    33001 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
    33061 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
    33121 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
    33181 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
    33241 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct
    33301 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
    33361 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
    33421 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
    33481 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
    33541 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgtg cgtaatcgtc
    33601 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
    33661 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
    33721 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
    33781 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
    33841 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
    33901 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
    33961 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
    34021 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
    34081 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
    34141 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg
    34201 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
    34261 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
    34321 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
    34381 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
    34441 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
    34501 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
    34561 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
    34621 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
    34681 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
    34741 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
    34801 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
    34861 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
    34921 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
    34981 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
    35041 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
    35101 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
    35161 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
    35221 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg
    35281 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
    35341 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
    35401 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
    35461 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
    35521 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
    35581 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
    35641 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
    35701 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
    35761 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag
    35821 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
    35881 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
    35941 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
    36001 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
    36061 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg
    36121 acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg
    36181 gcgaggatca catagcccat attccctgca gattcatgag agataaggtc ttcttgctct
    36241 gttggcatca ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg
    36301 tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg
    36361 atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg
    36421 acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg
    36481 tcgatggcat ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc
    36541 gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt
    36601 atgccgacgt taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg
    36661 ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc
    36721 tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc
    36781 ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta
    36841 cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat
    36901 ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca
    36961 tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagctgt gtcgttatag
    37021 cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa
    37081 gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt
    37141 ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt
    37201 gcggcactga tctgtgcggt actgtagctg ccatagcgac tgagaaaacc cccgcaagag
    37261 ttcatcagac ggtacgggac gttcgagttg gtgatatttc cgccatctac gcctgttccg
    37321 tacaggacat gtacagcctc attgccgtaa tctttcagga tccgccgaag attatcactg
    37381 atggtatcca gggcttcgtc ccaacttatc cgttcaaatt taccttcacc gcgctcgccg
    37441 acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg gatagagcgc
    37501 ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac
    37561 tccacccagt acacggtgtc atctttcaca tgcaaacgta gcagacagcg gctcccgcag
    37621 ttaacggtgc aggaactcca gaccgttttc tcttctaccg gagcctctgc cgcccggacc
    37681 atttgggaaa atggcagagt gaaagcactg cttgccagcg cgagactgcc aagtgcggag
    37741 gttttcatca gacttctatg gctgatttca gccttcatga gcgcctctgt ggtatggatt
    37801 ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta
    37861 gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt
    37921 ggtgaagggc gcagagtggt tagcgtattc acacatccgc catggtcgat tttatcgcca
    37981 gacatattgg gctcgtgcca ggctccctgg cccatagcgc taactccagg gagaatgcgt
    38041 ggtgttactt tggctggtag ccgaacttcg ccacgatggt taaacacccg caccatatcg
    38101 ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc ctgacggcag
    38161 gctgccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa
    38221 ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc aaaggttgag
    38281 gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt
    38341 tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc
    38401 tcggggtcgt cacgaaatgc tttgtaggcg acaaaatggc cattgggatc tttacgctta
    38461 tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt
    38521 ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag
    38581 acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg
    38641 aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg
    38701 ttaggaataa tatcttcctg ctcaacggtc atcaggtctt ccagcagaat gtcggcatat
    38761 tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc
    38821 tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt atttcccgcg
    38881 tagttccaga tggactccat tgccagacgg atggatggac ttgaagtagt tgactcaatg
    38941 ctacttaagg tcctgatatg actgacatta atcatgataa cactgtatat ccatccagtt
    39001 ctttctctgg gtctccctat cggtatcagc aagtctacac ctcaagccct gacacaaggc
    39061 tgacacaaat caatctactc agcgtagcta agcctcagtt agtccgcaga gccaatggtc
    39121 gatatactat ccgcttcaga ctcaagggac agaccacacc gtttctttca gtatcgaccc
    39181 gcagcacaga caggagagta gccacaatgc gtcagagaga gcttgcagcc accgctaagg
    39241 ccttcatgtt ggataggcct gaagtctcac tacaggagct tacagagcat ctccggtcta
    39301 tggctgagca gttcctgaca gacgctagtg acgattactg gaatggactt gaggtcgcta
    39361 ctctggtcga cgagaagtcc aacctgaagg agctagccgc tacgcaagcc ttgagtctgg
    39421 accagcagaa gggcataagg ttagcgctgg aagtcctgac cgcagcacag cagagagtcg
    39481 acactggtga tacctctggt ctgattaagc tgattgatga taataacctt actgatgact
    39541 caactatagg cgactctacg tcaattctga ataatgagca gggggataga ccggcagttt
    39601 tcacacagga gcgccagtca tctgtagttt tttcaagcct agtgtcctcg ctgctggctg
    39661 agaaggtcca gaccctgaag acatccagct acaaggacct ctcgtcctca ctcaatactg
    39721 tctcccggtt cctgcctgaa gacatggacc tgatgtcccg ctctgggtgg ctggctgtca
    39781 gggactctat gctggcttct gaggtgagac catcgacaat caacaagctg ctgaccaaag
    39841 cgaagatgtg tctcgattat ggcctgatga atgggcaact tgagggccgc aaccctattg
    39901 aacggatgaa gctgaccaag gatatcgact ccaagcgcag agcgttcaca gacgaggagc
    39961 tggagagact tctggtccgt gttgagtctg
//
LOCUS       DAJVQR010000029        38777 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVQR010000029 DAJVQR010000000
VERSION     DAJVQR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..238)
                     /gene="yefM"
                     /locus_tag="OQM52_001594"
     CDS             complement(<1..238)
                     /gene="yefM"
                     /locus_tag="OQM52_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1563488.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEK"
     gene            521..562
                     /gene="hisL"
                     /locus_tag="OQM52_001595"
     CDS             521..562
                     /gene="hisL"
                     /locus_tag="OQM52_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1563489.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    538..661
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            708..1607
                     /gene="hisG"
                     /locus_tag="OQM52_001596"
     CDS             708..1607
                     /gene="hisG"
                     /locus_tag="OQM52_001596"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1563490.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1613..2917
                     /gene="hisD"
                     /locus_tag="OQM52_001597"
     CDS             1613..2917
                     /gene="hisD"
                     /locus_tag="OQM52_001597"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1563491.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2914..3984
                     /gene="hisC"
                     /locus_tag="OQM52_001598"
     CDS             2914..3984
                     /gene="hisC"
                     /locus_tag="OQM52_001598"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1563492.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3984..5051
                     /gene="hisB"
                     /locus_tag="OQM52_001599"
     CDS             3984..5051
                     /gene="hisB"
                     /locus_tag="OQM52_001599"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1563493.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5051..5641
                     /gene="hisH"
                     /locus_tag="OQM52_001600"
     CDS             5051..5641
                     /gene="hisH"
                     /locus_tag="OQM52_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1563494.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5641..6378
                     /gene="hisA"
                     /locus_tag="OQM52_001601"
     CDS             5641..6378
                     /gene="hisA"
                     /locus_tag="OQM52_001601"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1563495.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6360..7136
                     /gene="hisF"
                     /locus_tag="OQM52_001602"
     CDS             6360..7136
                     /gene="hisF"
                     /locus_tag="OQM52_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1563496.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7130..7741
                     /locus_tag="OQM52_001603"
     CDS             7130..7741
                     /locus_tag="OQM52_001603"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1563497.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7927..8904)
                     /gene="wzzB"
                     /locus_tag="OQM52_001604"
     CDS             complement(7927..8904)
                     /gene="wzzB"
                     /locus_tag="OQM52_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1563498.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9050..10170)
                     /gene="ugd"
                     /locus_tag="OQM52_001605"
                     /pseudo
     CDS             complement(9050..10170)
                     /gene="ugd"
                     /locus_tag="OQM52_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10420..11826)
                     /gene="gndA"
                     /locus_tag="OQM52_001606"
     CDS             complement(10420..11826)
                     /gene="gndA"
                     /locus_tag="OQM52_001606"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1563499.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11935..12294)
                     /locus_tag="OQM52_001607"
     CDS             complement(11935..12294)
                     /locus_tag="OQM52_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563500.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12291..12797)
                     /locus_tag="OQM52_001608"
     CDS             complement(12291..12797)
                     /locus_tag="OQM52_001608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563501.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12943..13506)
                     /locus_tag="OQM52_001609"
     CDS             complement(12943..13506)
                     /locus_tag="OQM52_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563502.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13713..14651)
                     /locus_tag="OQM52_001610"
                     /pseudo
     CDS             complement(13713..14651)
                     /locus_tag="OQM52_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14714..15088)
                     /locus_tag="OQM52_001611"
     CDS             complement(14714..15088)
                     /locus_tag="OQM52_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCS1563503.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15092..16240)
                     /gene="wzy"
                     /locus_tag="OQM52_001612"
     CDS             complement(15092..16240)
                     /gene="wzy"
                     /locus_tag="OQM52_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCS1563504.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16260..17165)
                     /gene="rfbG"
                     /locus_tag="OQM52_001613"
     CDS             complement(16260..17165)
                     /gene="rfbG"
                     /locus_tag="OQM52_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCS1563505.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17158..18048)
                     /locus_tag="OQM52_001614"
     CDS             complement(17158..18048)
                     /locus_tag="OQM52_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCS1563506.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18078..19334)
                     /gene="wzx"
                     /locus_tag="OQM52_001615"
     CDS             complement(18078..19334)
                     /gene="wzx"
                     /locus_tag="OQM52_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCS1563507.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19350..19895)
                     /gene="rfbC"
                     /locus_tag="OQM52_001616"
     CDS             complement(19350..19895)
                     /gene="rfbC"
                     /locus_tag="OQM52_001616"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1563508.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19900..20778)
                     /gene="rfbA"
                     /locus_tag="OQM52_001617"
     CDS             complement(19900..20778)
                     /gene="rfbA"
                     /locus_tag="OQM52_001617"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1563509.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20837..21736)
                     /gene="rfbD"
                     /locus_tag="OQM52_001618"
     CDS             complement(20837..21736)
                     /gene="rfbD"
                     /locus_tag="OQM52_001618"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1563510.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21736..22821)
                     /gene="rfbB"
                     /locus_tag="OQM52_001619"
     CDS             complement(21736..22821)
                     /gene="rfbB"
                     /locus_tag="OQM52_001619"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1563511.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23194..24087)
                     /gene="galF"
                     /locus_tag="OQM52_001620"
     CDS             complement(23194..24087)
                     /gene="galF"
                     /locus_tag="OQM52_001620"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1563512.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24262..25656)
                     /gene="wcaM"
                     /locus_tag="OQM52_001621"
     CDS             complement(24262..25656)
                     /gene="wcaM"
                     /locus_tag="OQM52_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1563513.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25667..26887)
                     /gene="wcaL"
                     /locus_tag="OQM52_001622"
     CDS             complement(25667..26887)
                     /gene="wcaL"
                     /locus_tag="OQM52_001622"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1563514.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26884..28164)
                     /gene="wcaK"
                     /locus_tag="OQM52_001623"
     CDS             complement(26884..28164)
                     /gene="wcaK"
                     /locus_tag="OQM52_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1563515.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28344..29822)
                     /gene="wzxC"
                     /locus_tag="OQM52_001624"
     CDS             complement(28344..29822)
                     /gene="wzxC"
                     /locus_tag="OQM52_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1563516.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29824..31218)
                     /gene="wcaJ"
                     /locus_tag="OQM52_001625"
     CDS             complement(29824..31218)
                     /gene="wcaJ"
                     /locus_tag="OQM52_001625"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1563517.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31273..32643)
                     /gene="cpsG"
                     /locus_tag="OQM52_001626"
                     /gene_synonym="manB"
     CDS             complement(31273..32643)
                     /gene="cpsG"
                     /locus_tag="OQM52_001626"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1563518.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32836..34272)
                     /gene="cpsB"
                     /locus_tag="OQM52_001627"
     CDS             complement(32836..34272)
                     /gene="cpsB"
                     /locus_tag="OQM52_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1563519.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34275..35498)
                     /gene="wcaI"
                     /locus_tag="OQM52_001628"
     CDS             complement(34275..35498)
                     /gene="wcaI"
                     /locus_tag="OQM52_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1563520.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35495..35974)
                     /gene="gmm"
                     /locus_tag="OQM52_001629"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35495..35974)
                     /gene="gmm"
                     /locus_tag="OQM52_001629"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1563521.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35974..36942)
                     /gene="fcl"
                     /locus_tag="OQM52_001630"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35974..36942)
                     /gene="fcl"
                     /locus_tag="OQM52_001630"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS1563522.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36945..38066)
                     /gene="gmd"
                     /locus_tag="OQM52_001631"
     CDS             complement(36945..38066)
                     /gene="gmd"
                     /locus_tag="OQM52_001631"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1563523.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38092..38640)
                     /gene="wcaF"
                     /locus_tag="OQM52_001632"
     CDS             complement(38092..38640)
                     /gene="wcaF"
                     /locus_tag="OQM52_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1563524.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38656..>38777)
                     /locus_tag="OQM52_001633"
     CDS             complement(38656..>38777)
                     /locus_tag="OQM52_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1563525.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10350 a         9713 c         8728 g         9986 t
ORIGIN      
        1 ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
       61 tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct tcgagtgaca
      121 tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga tcttcaacgg
      181 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
      241 aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac tatttattaa
      301 ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc tctttttggt
      361 gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca
      421 aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg
      481 ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg ttcaatttaa
      541 acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca ttcagatctt
      601 ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg ggctttttta
      661 ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg acagacaaca
      721 ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca cgcgaattac
      781 tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg atggcagaaa
      841 acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg gtaatggacg
      901 gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg cttaaccgcc
      961 gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc ggcggctgcc
     1021 gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc ttaaacggta
     1081 aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag aaaggtatct
     1141 cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc gggctggcgg
     1201 atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc ctgcgcgaag
     1261 tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa atggaagaat
     1321 ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc caggcgcgcg
     1381 aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc atcgccctgc
     1441 tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag cgcgtagcga
     1501 tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg aaagcactgg
     1561 gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg ccatgagctt
     1621 taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc tgttaatgcg
     1681 cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc tcgataacgt
     1741 gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata aaaccacggt
     1801 taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc tgagcgacga
     1861 gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca ctgcgcaaaa
     1921 actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg tcacgcgccc
     1981 ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct caacggtatt
     2041 aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt gctcaccgcc
     2101 gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg acgtgtttaa
     2161 cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt
     2221 ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc aggtaagcca
     2281 gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc tggtgattgc
     2341 tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg ctgaacacgg
     2401 ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc gcgttgccga
     2461 ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc aggcactgaa
     2521 cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct ccaaccagta
     2581 cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg atggcatcac
     2641 cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg actacgcctc
     2701 cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca gcctcgggct
     2761 ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt tctccgcgct
     2821 ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca aaaatgccgt
     2881 tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat tactgattta
     2941 gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct gggcggtaac
     3001 ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa gcttactcag
     3061 caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa ttacgcgcaa
     3121 tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga aggtattgaa
     3181 ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg cccgccaacg
     3241 tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac agtgccgacg
     3301 ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg cgtaaaagtg
     3361 gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca ggattttcgc
     3421 accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc ttatatcgag
     3481 ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct ggcgattttg
     3541 cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac gctggcaaac
     3601 gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc gacgccggtt
     3661 gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag cgaacgggtg
     3721 gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat cccctgcgtg
     3781 gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc ctccagtgca
     3841 gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa acaaccctct
     3901 ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg cgtcattgac
     3961 gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat cgcgatggaa
     4021 ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa ctcgcctttg
     4081 aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag ctggtgatga
     4141 tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt gatggcccgc
     4201 acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa gtgctgattt
     4261 gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa ctggtggagg
     4321 gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc gatcgcgcga
     4381 ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac cgcgaaaccc
     4441 tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc catgtagtgc
     4501 gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa ggtggcagca
     4561 agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc acccacggcg
     4621 gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac cacaccgtcg
     4681 aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac aaacgcggta
     4741 tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc gcactggata
     4801 tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt gtgggcgatc
     4861 tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg ggcgtgacac
     4921 tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg ttcaaagcct
     4981 ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc tcgtcgaaag
     5041 gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga actctgtgaa
     5101 gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg acattgtgtt
     5161 gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga tggatcaggt
     5221 gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc tgggcatctg
     5281 cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg acttgctggg
     5341 catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc cacatatggg
     5401 ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg aagacggcgc
     5461 gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca tcgcccagtg
     5521 taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt
     5581 ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc tggagatgtg
     5641 atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca tcagggcgat
     5701 tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga ttacgctgcg
     5761 caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc ggctaagcgt
     5821 caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca ggttggcggc
     5881 ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc gcgcgtggtg
     5941 gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga acgtttcggt
     6001 gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa caagcaagtg
     6061 gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt ggaaacctat
     6121 ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg cacgctggcg
     6181 ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt ggcgtttcag
     6241 tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac tggcgtgcgc
     6301 ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga ggccatcgca
     6361 tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg gtgaaaggcg
     6421 tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa cgctacgccg
     6481 aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg
     6541 tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt tgtgtggcgg
     6601 gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg gataaaattt
     6661 ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc gatcgctttg
     6721 gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc ggtaaatatc
     6781 atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg gaaacgctcg
     6841 actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac atgatgaatc
     6901 aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt gaagtttgcc
     6961 acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg gaagccttcc
     7021 gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa ataatcaata
     7081 ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata tgttaacaga
     7141 acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg tgattgtgca
     7201 acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag ccttagacaa
     7261 aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac tgtggatcaa
     7321 aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact gcgacaacga
     7381 cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca ccagtagctg
     7441 cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac tgctcgccga
     7501 acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca gcggcactaa
     7561 acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag caacggtaca
     7621 tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc tggtgctgtt
     7681 gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac ggcatcagtg
     7741 agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc ccgatgcgca
     7801 aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg cgccagcatc
     7861 gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct taaatgcaca
     7921 ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa tccccgcgcc
     7981 aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg gactatcgcg
     8041 acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa gatcatcaaa
     8101 ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg gtgagaacac
     8161 caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc ctagaaggaa
     8221 caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg
     8281 attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct gctcctgcgc
     8341 gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag caatgttgtc
     8401 cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa tgtattgggc
     8461 caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca atggtaattg
     8521 ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat tatccagcgt
     8581 ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct gcaaatccga
     8641 tactttcggt gcagcctgac catagataac attcatggca ttgttatagc cagcaatttg
     8701 ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg ctactgccaa
     8761 ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca tcttgccacg
     8821 ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt tttgcccaga
     8881 aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc aagtccgcat
     8941 agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat gaattattgc
     9001 ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt tagtcgctgc
     9061 caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct gccatacggt
     9121 tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc aggcgagagt
     9181 tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg cctttcgcct
     9241 tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac ccgctcttca
     9301 taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg agatcaggtt
     9361 attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca gacaataacc
     9421 accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga gacaaacgcc
     9481 ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca gttcattaaa
     9541 gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt ctgcttcagt
     9601 ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc
     9661 aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat ggagattatc
     9721 gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt cagtacgata
     9781 tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga ccataaccgc
     9841 ataaggattt atctcaacta cgtctttaat tactgattct acactggatg tattgaaata
     9901 attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag catcccggta
     9961 ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca aaaactgctg
    10021 aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc gtgacggtaa
    10081 aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg acaagcctac
    10141 atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac ttaactgtga
    10201 atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac gttgtcagat
    10261 ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat gtttattaag
    10321 ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag cccggtgcaa
    10381 tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc attcagtatg
    10441 gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt cacgctgtgc
    10501 ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg caaccgcagc
    10561 ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat cgcgcagcgc
    10621 ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt tagcgatctg
    10681 cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga tgatgcagcc
    10741 agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt cttcagacgc
    10801 agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat acagcgcact
    10861 gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg gaccagagag
    10921 aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg caaacacaga
    10981 ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg tccatttacc
    11041 ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac cgtcttcatc
    11101 ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt caccgttatt
    11161 ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac ctttgagcag
    11221 agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt gaaccatctt
    11281 cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc cgtcttctgc
    11341 cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt tctgcccgcc
    11401 aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac cggtaccgat
    11461 gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct ggaagaaggt
    11521 attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg aatcaatcgc
    11581 agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg tttccagaga
    11641 ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat tttcagcaat
    11701 cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac cacgactttc
    11761 gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga tctgttgctt
    11821 tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt atattattaa
    11881 ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg cttcttactt
    11941 tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg gatacttttc
    12001 tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat taaaagattt
    12061 catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac taatagtcca
    12121 atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat agaagtaaat
    12181 accatcctcc aagcctaatt ggataagatt atctccatat aactcactat attcacttgc
    12241 tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat tcaagtattc
    12301 agaataaaaa tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaat aatacacaca
    12361 aggataaaat atcgtcccaa cttgtaattt gtaatgttga tatcttttat taaacttgca
    12421 attgagatcg caacctgcaa tactgacaat gggtaaatgg gtgataaata cctgtcacct
    12481 tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa tacccaaagt
    12541 attttattat tcctaagtgt aaagacagac agtatagcat aaaaaacaac atcactgcca
    12601 actttattaa ttgcttgttg caaatctaat gtggcaggac ttataccatg tcgagcttgt
    12661 tctgctgttt gaacttgtag atcaatttca ttccataggt agtgaccaaa tatatttcct
    12721 ccagcaggac taaaaggatc accagagatc caaaaactac ggacatacca ataaatgcca
    12781 ccaagaattg ttgccaagct aaaaataaga aatgtctgta tgtttctctt tttcgctatc
    12841 aatataacaa atatgatggc tccatatatg gcaccaaata gttttacgcc acaagcaatt
    12901 ccaataaaaa aaccacataa aaaaattaat tcattttgtg ttttattgtt ttctgagcga
    12961 atatatataa gaatactggc tgcagcaaaa caaagatagc gttaaatcaa tatatgcata
    13021 accaagatga tctttaagtg gattgatagt aaatagtagg attgtagcaa taatgcctgg
    13081 ataataagtt ttcgtaattt cccatgaaca gccaacaagt ccacaggcta ttacaaaaaa
    13141 acagatattt gctaaaaact gagctaacct tacatctcct aactgtaaac ctaaagccat
    13201 taaaagatct gcattttgcg ggaacagagg aaaacgcaaa tattgttcaa caacaattgc
    13261 atggtgctca actaatgaac gagccaaagg taagtgatac atagtatcat cccaatcacc
    13321 gggagctcgc agggacgata acaatgtaac cacaaaaatc aaaacaagaa ataaaataac
    13381 aaacagcgta ctgaactcaa tattatttat tgccagccat ctaaattgct gacgtttaat
    13441 aatgcttaaa acccaacaca ccgtaaaaat taaaaagata gcaatcttat taaattgccc
    13501 gaccatgccc agaaagtaaa ttatcaaaat atttaaagac atccctatgc ataggcaata
    13561 aaaaataaag aaaatttgtt aagtcctgcc accccctcct taaaaaaaga tgacaataca
    13621 atattaccaa tgacaaaaaa tgtcattcca aaagcaaaac aatacacaac atacaaatac
    13681 aacggcaatt caatcatagt aaaatactct atttacatta tcagtttata gtttttttca
    13741 gaaagataaa ataatgcctt catttcacgt ctagttgttg tatttgaatg caaaataatg
    13801 ccacatacaa atgccattat tgacgataac atgatggagg cagttaaaaa ggcagtcgga
    13861 aatcttctca ccaaacctgt atcaatataa tcaacaatga tgggcaatcc taaaattata
    13921 gacaaagcag caagtaaaac gccgataata ctaaaaaagt atagtggccg ttcaagcgag
    13981 taaagtttaa ttattgtttt cagaatcttg aacccatcac tccaagttga tagtttgctc
    14041 acactccctt cagatcgctc gccatatttt gtattaacct caccatactt catacgtaat
    14101 tctaaagcat gaattgtaag ctcagtttcg atttcaaatc cccttgatag acatggaaaa
    14161 gatttaacat agcgtctgga aaatacacga taccccgaca acatatcagt aaaattccct
    14221 ttaaagatag tctgaacagt accagtcaac aatttatttc ctcagcgatg cccttttctg
    14281 tatgtatttt gatctccatt ctcttgacga catcctacaa ccatatccaa ttgatttttt
    14341 atgagatggt taatcatttc tggagcagaa cttgcatcat aagtgtcatc tccatcaacc
    14401 attagataaa tatccgcatc tacatctgag aacatacgtc ttacgacatt ccccttgcct
    14461 ttcagaggaa cagagtgaac tgttgctcca gcatcctctg ccacttgtgc tgtactgtca
    14521 gtcgaattat tatcaaatac atatatagaa gcagttggaa tattcgtttt gaaatcattt
    14581 atgacttttg ctattgcctt ctgctcatta taacaaggaa taattacagc gatattaaaa
    14641 gagtgataca taagcccctc atataaacta tttagctaat gtgcaagtaa ttgtgttttt
    14701 aacaaatcgt ttactaacga aaaatgaaat attttgatgc gcaatatcct aaaaccaaac
    14761 taaaaagaga aaaggtgaac aatgtaaata ttggagataa agctaagaga tcaaataaaa
    14821 cgccgaataa ataactcatc gcccccataa aaaatataaa tataaaatat ctagccttgg
    14881 ttgcagatac tttaaatgag catcttgcat ttgcaaaaaa agaaaaggtt gctgcacata
    14941 taaaaccaac aatgttactt cggccttgac caaaagctaa attattataa cacgcataaa
    15001 atacccccca atgtaaagca gtattgacaa ttccaattaa aaaataacta aaaaaagaac
    15061 tggtcaataa cttccctatt tttaacatcc tttattttgc tccagaagtg aggttattac
    15121 taatttggat attttctata gaaaataccc ataaaactac aacgcctata ataaaaattc
    15181 ccatgggtgt aaaaagaaat ggattagtta tagaaacggt aatactgaaa aaaagtatta
    15241 acgacatcat taatgatgtt tttgtactat ttaaaaaata gcctttcaaa aagataagca
    15301 acataggaac taatgatata aatacaccaa taacgcctgt tttctgaagt atctcaagag
    15361 gtactatttc aatccttaat cgatctaata taaatgaacc aaatcctctt ccaaacaaga
    15421 acgtcgctaa atcaacattt ttataataaa aatataaatc attaaatcta acagagtcag
    15481 aatcttcact gcgtgatccg accatgtaaa gcataattat aaatagaacg ctgcccaata
    15541 ttattataat agtatttaaa tatagcttat gtaatttaaa actggctaat aacagtgata
    15601 atgttgtaaa aagtaataca cctcttgatt ctgtcaataa tatcgcaagc actattatga
    15661 gacattttaa atattttttg tcgacaaaac tgataatcgc accgacacca aaaaagtaga
    15721 aggatttgga aaaaaaagcc ccatcgggac gaaaaaagaa ctcgctagtc aatgataagt
    15781 gttcataaat taaattaaaa tttaacaaac cgaaattcaa caaaattatg tatgaaatat
    15841 ataacagtga tccatataat gcaaatgcat taactatttt aataaaaatc tcacatgaaa
    15901 atacttttaa agtatatctt agtgtaaata gatatataag tgacagcata aaaaggtatg
    15961 gttgcacatc aaacagtata tcatttattt tattaccagc taataagccg acaaagaagc
    16021 cataaaaagg aaataaaagc aaccatataa aaagactgac acatttttgg gtgattgaaa
    16081 aattgaatgt aactaaatta aaaattaaaa gtataaaact aaatacaaat aataaatatc
    16141 gcaaagggaa tattcccaat ggctccagta atcttccacc accaccaata atcagttcaa
    16201 tacataaaaa tgttataaaa attttattta tattattcat attcgtaagg tgatgttttt
    16261 taaaatatgt ttaaaatcaa cattaataaa atataaattt tatcagcctt tttccgcctt
    16321 aacttccaaa aacttaaagc caagatcaaa ttgcctatat aacctttacc caattgatta
    16381 ttcacaaagc tatcatcagc taaggcatta gctattttag ttacttcttt tcgataccac
    16441 ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt tgctcccact
    16501 tggttgtttt catgctgtct gtacagcatt gttggttttc tatcaatata ccacgaataa
    16561 tttctcgtcc gcgcaaatga atataaaaac caatcatgca gacagatatc ttgtgcagca
    16621 tttttattat ttatgatgaa cttcttaaat tcaataagcg tttctttttt tataacatat
    16681 gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga ctgagcctta
    16741 tcaactaaat tttttcgccc tgatggataa tatgcaatta cattgctgga ataacaattc
    16801 gcattattaa ataccaagca gtcaattgca ttttttattt tatctttata ccaaatatcg
    16861 tcctgatcac taaaacagat ataatcatag ttttcaagat cgacatcttt aattaatcta
    16921 taaaaatttt tacctgcccc cccaaactta ttgacgcttg gaagtatatt tataattgag
    16981 ggatatgata attgaaattc ttcacaaata tttaatgtat tatcagttga taaatcatct
    17041 gatataaaaa ttgagatgtc tacatctttt tgttcaataa tactttttaa ttgttcctca
    17101 atccagcatt cgccattata cgtagccata agtacagcta ctcttttttg atcagaacta
    17161 tgcattataa cgaccgcccc cagtaattcc tcttattcca tccagcgtag ccaaaatatg
    17221 ttttttcata tattcaattt tatttttttg agttgcgata attaaagtcg tatgaattaa
    17281 agaaaaaaca acttccctaa tagagagtaa aagaggcaca tgattttttc taagcaaaag
    17341 aaatgcattt cttactcgat aataacgacg tactggcgag tgaattggta tgttaatccc
    17401 acagattttt actgacttat ccccaatagc atgaaccatt tctatatcag gaatgatatg
    17461 aactaatatg ccatagttta agcatctaag acaccattct gtatcgacat aatcaataaa
    17521 aagttcctca tccatcattc caacaatctc aaaaacttct tttgaaacca tggttccgga
    17581 tgagattgtt actgaacttt taaacggtgt ctgtccttca gtgacatgaa ttttctctcg
    17641 aagaccattt tttttgatat tacaaatagg ataataatgt gacttatctc tatcaataaa
    17701 aactgggcca gctatttttg cattttcatt atccatatag caggaaagtt ttttgataaa
    17761 ctcattgcga atacggctat cttgatcaaa aaaaattatc ttctctgccc ctttttcaag
    17821 agaatatcga atcccaatat tttgtgcttt tgctatacct tcattcctac caagacaaat
    17881 taaatgaact ttttcaatgt ctgctaattt atctttcaag gtgaaattag tcctattatc
    17941 gactactaca acgtaacaat tttgctcctt tagttcagtg atcagtgcat tcagattttt
    18001 aagttcggga ttatatgtca caatgacagc gtaaatatta ctattcatta tccaagtgac
    18061 tcagtaattg gttaatttta gttttcatat acagaacaca ttctagtata aagccattgt
    18121 aataacagag catctaaaat tacacgtatg ctccaggata ttgcggcacc cagtaaccca
    18181 tatttttcga ccataaaata caaaagggct aaataaggaa ccaattcagc acaatgaatc
    18241 aaagcagtaa ttttagattt tcctaatgcc tggattgcag agaatggtat cattgccagc
    18301 gcattgaaga aaaaaccgat caataacaca tttaatatat taaaggaatt aatttgataa
    18361 ttctcaccaa accatatttt caatacaagc cctgaatata acaagcctat gattatcacc
    18421 ggcaaacaga ttagaaacat taaaagcaat gatttattta cattacgttt aaattcttta
    18481 aagtctttga tgttacttaa ccttggaaac actgccctcc cgattgctgc aggtattatt
    18541 cctaatttta gaataacctc cgctggtgca gaataaaatg caactttatc tgcccccata
    18601 atatttgaca ctataaatcg atcaaaatac accatgaccg gacttatgat attacttact
    18661 gtcatccaac cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa atgaaccccc
    18721 gaaattttaa tatcattcct gacataatag gcagagacta aaatcgaaat aactcttgca
    18781 aatattaaac cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa tatggccgga
    18841 attcccgcga tgcaagagct tgatatagat ttttgaatat ttacaatgcc aaatttttca
    18901 tccccctcaa gaatggctga ccataattga tttagaataa ataatggaat gcaaatagcc
    18961 aacagtttaa atgctagttg tatatcacta tgttcaacac cagaaatttt tagataatta
    19021 acaattccat cagagaaaat caataataaa aaagcaccaa agcatgaaaa tagcaccaaa
    19081 aaagatgtac ttgttgaaat taccttggtt ctttcatgat gattatcacg atgaattgca
    19141 atttctctaa taacagagcg agtcaggcct acatcaaaaa ttccagcata cccaacaagt
    19201 gcaattgcaa ttgtataaac accgaaacct tcaggcccca aacctcgagc gagaaatcct
    19261 agcgagggga tagcaattaa agttggtatt gcatagccaa aaaggttcca gacactattt
    19321 tttattatgc tcattattac atttctttat catataaaat aatcagcatc tacaaatttt
    19381 tgaccattaa tatctttatc agaaagcaat ggattttgaa ccggccattc tatatttata
    19441 cttttgtcgc tccataaaat cccacgctct tgcatttttg aatagaaatt atttgttttg
    19501 tagacaaatt ctgcttcatc actaagaacg caaaacccat gagcgaatcc ttccggtatc
    19561 cataattgac gcttattctc cgccgacaaa tttacaccaa cccacttgcc aaatgttggt
    19621 gatgatttcc gaatatcaac agctacgtca aaaacttcac cgacaacaca gcgtaccagt
    19681 tttccttgag cataaggttc cagctgatag tgcagtccgc gtaacacacc cttagttgat
    19741 ttagaatggt tatcctgaac aaactcaacc tttcgtccta cagcctcttc gaaaactttc
    19801 tgattgaagc tttccataaa aaagccccgc tcatcaccaa atacttttgg ttcaaaaatt
    19861 agcacatcag gaatttcagt tttaattacg ttcattttat taataacctt taatcatttt
    19921 cagcagatac tgaccataag catttttctt cagcggctcg gctaatacct tcacttgctc
    19981 agcatcaata aagcctttac ggtgagcaat ttcttccggg caggaaactt tcagcccctg
    20041 acgctcttca attgttgcaa tgaagttgct tgcttcaata agactttgat gtgtccccgt
    20101 gtccagccat gcataaccac gccccatcat agcgacagac aaacgtcctt ggtccatata
    20161 aatacggtta atatcggtaa tttccagttc gccacgtgca gatggtttaa ggtttttcgc
    20221 catttctaca acgtcattgt catagaaata aagcccagta accgcatagt tactttttgg
    20281 ttcctgcggt ttttcttcca gactaattgc cgtaccgtta tcatcaaatt ccaccacacc
    20341 gtagcgctca ggatcattta cgtgataggc aaaaaccgtt gcgccactct ctctgttaac
    20401 agcggtatcc attaatttcg gcaggtcgtg accatagaag atattatcac caagtaccag
    20461 cgcacaatca tcaccaccta taaattcttc accaatgata aatgcttgcg ccagaccatc
    20521 cggactcggt tgtactttat attgcagatt aagcccccac tggcttccat caccaagcaa
    20581 ttgttgaaaa cgtggtgtat cctgcggcgt actgataatc agaatatctc gaatacccgc
    20641 caacataagc gttgaaagcg gataataaat catcggttta tcataaatcg gtagcagttg
    20701 tttgctcaca gccattgtca caggataaag acgagtgccg gaaccaccag ccagaataat
    20761 acctttacgc gttttcattt caccattcct tttaattcat ctcgctctag catcatgagc
    20821 gagatgcaat aatttgttaa attgccgtgg tcgtaaataa ttcgttgagc atacgtttca
    20881 cgcccacctg ccagtcaggc aagacaagcg caaagttctg ctgaaatttt tcggtattga
    20941 ggcgagaatt atggggacga cgtgctggtg tgggataggc cgttgtcggc acggcattaa
    21001 gtttgttaag agcaaggttg atccccgctc tgcgcgcttc ttcaaatacc agagcggcat
    21061 aatcgtgcca ggttgttgta ccaccagcaa ccagatggta caaaccagca acttctggtt
    21121 tatttgcggc cacgcgaatg gcatgagcgg tgcaatcagc cagtaattca gcccccgttg
    21181 gtgcgccgaa ttgatcgttt attacagcca actcttcgcg ttcttttgcc aggcgcaaca
    21241 tcgttttggc aaagttattt cctttaccag cgtataccca gctggtacgg aaaataagat
    21301 gctttgcgca atgttcttgt aacgcttttt ccccagctag cttggtttca ccgtagacat
    21361 ttagcggtgc tgttgcatcc gtttccagcc atggcgtgtc accgttgcct gggaaaacat
    21421 aatcagtgga gtagtgtata acccaggctc cgacttcatt agctgctttt gcaatcgctt
    21481 cgacgcatgt cgcgttaagt aattgcgcga aattcggttc tgattccgct ttgtctactg
    21541 cagtgtgagc agcagcatta acaataacat cggggcgaat ttttttgacg gtttcagcca
    21601 caccttcggg gttgctgaaa tctccacaat aatcagtgga gtgaacatcg agcgcaatca
    21661 agttacccag aggagccaga gcacgctgca gttcccaacc cacctgccct gttttgccga
    21721 aaagcaggat attcattact gacggccctc atagttttgt tcaatccacg actgataagt
    21781 accgcttttc acattctcaa cccaatttgt attagccaga taccattcca ccgttttacg
    21841 aatcccgctc tcaaacgttt cttgcggttt ccagcccaat tcgcggctaa ttttatcggc
    21901 atcaatggcg taacggcggt catgccccgg gcgatcggca acataagtaa tttgctcacg
    21961 ataagatttc tctttcggta caatctcatc caacaaatca caaatagtga gcactacgtc
    22021 gatgtttttc ttttcgttat gtccaccaat gttataagtt tcacccgctt taccttcggt
    22081 tacgacggta tataacgcac gagcatgatc ctctacatac aaccagtcgc gaatttgatc
    22141 ccctttacca taaataggta atgccttacc ttccagagca ttcagaataa ccaatggaat
    22201 aagtttttcc gggaagtgat aaggaccata attgttcgag caattagtca caatggttgg
    22261 taaaccataa gtccgcttcc aggcacgtac caaatgatcg ctggaagctt tagacgcaga
    22321 atacgggcta cttggcgcgt aagctgtcgt ttccgtaaat agcggtaacg cttcgttatt
    22381 atttgcttca tccggatggg gtaagtcacc ataaacttca tcagtagaaa tatgatgaaa
    22441 gcggaaactt tttttctttt catcattcag agcagaccag taattgcgcg ccgcttctaa
    22501 aagaacataa gtccccacaa tattggtttc aataaatgcc gcagggcccg ttattgaacg
    22561 gtcaacgtgg ctctctgccg ccaggtgcat caccgcgtct ggctggtgct gtgcgaaaat
    22621 acgcgacatg gctacagcat cgcagatatc tgcatgctca aaagcataac gttcagaatc
    22681 agaaacatca gcgagtgatt ccaggttccc ggcgtatgtt aatttatcga cattaacaac
    22741 actatcttgc gtattattta ttatgtgacg aacaacagca gaaccaataa accctgcgcc
    22801 accagtaaca agtatcttca cttttttatt ccatatagct atagagcatg ctgtgaaata
    22861 gactgctcca gatttgatta atagaggtat taatacacgc taccgcccct ggcttaacag
    22921 ctaccagtgc actgcatgca tctcaacgat gtggcgagcg ttagccactc gcgccaaatc
    22981 cgaaaaattc aaacgttaat tgtcttacca aacagccgta gaaacaagga aaatcctgga
    23041 aaaatttgaa taatatgagc tatgtaactt cttgttttta taggctttat aaaaacggca
    23101 acggcaggaa aattcaatga gcaaaactta tgccacgaat cttcactgcc gttataattt
    23161 tcttatcaac cgttacatcc ggtcagattt tcattattcg cttaacagct tctcaatacc
    23221 tttacggaac ttcgcccctt ctttcaggtt gcgtaggcca tacttcacaa acgcctgcat
    23281 atagcccatt tttttgccgc agtcgtaact gtcgccggtc atcagcattg catcaacgga
    23341 ttgttttttc gccagctcgg caatagcatc agtcagctga atacgtcccc atgcaccagg
    23401 ctgagtacgt tccagttccg gccaaatatc ggcagaaagc acatagcgac ctacggccat
    23461 gatgtctgag tccagcgtct gcggctgatc cggtttttcg ataaattcaa caatgcggct
    23521 gactttaccc tcacggtcca gcggctcttt agtctggatg acggagtatt cagagaggtc
    23581 acccggcata cgttttgcca gcacctggct gcggcccgtt tcgttgaaac gtgcaatcat
    23641 ggcagcaagg ttgtaacgta gcgggtcggc gctggcatcg tcgatcacaa cgtctggcag
    23701 taccacgaca aatgggttgt caccaatggc aggtcgcgca cacaaaatgg agtggcctaa
    23761 acctaaaggt tcgccctgac gcacgttcat aatgatcacg cccggcggac agatggactg
    23821 tacttccgcc agcagttgac gcttcacgcg ctgctcaagg agtgattcta actcataaga
    23881 ggtgtcgaag tggttttcga ccgcgttctt ggacgcgtga gttaccagga ggatttcttt
    23941 gatccctgca gccacaatct cgtcaacaat gtactgaatc attggcttgt cgacgattgg
    24001 tagcatctct ttgggtatcg ccttagtggc aggcaacata tgcatcccga gacccgctac
    24061 aggaataact gcttttaaat tcgtcattat ttcatccacc tgtaaaatgg ttgctgaatt
    24121 atagcttgtt cgattttttt cgccagcatc aattaccctg aattgattac tgaattactt
    24181 gtgatgttac gccgcttcgt tgtggattgc agtagcattg ttcctaagta tggctccatt
    24241 tttccaggaa tggtcgcaaa tctactccct ccgttccggc aatctaaagt taatcttttc
    24301 cacattaaca atatggtgat taatcctgtc gatatcgacg gagctttgcc ctctttcatt
    24361 caccgcatga acatttgcaa gagacagcag tgtttctttt ttcgccataa agacgccgcg
    24421 aacgtctttg cgcatgtcga agttcatgct caatgctggt ccaactgagg attcctgcat
    24481 cacattgata tttcgcataa aaagatgttg cggcttgttg tgtaactcca gcgaggcacg
    24541 cttcatctca atgttagtta gcgacacaaa ggagacggca ttcccggcgg agatttggat
    24601 gccgcgcaat ttataagcaa gatgggtatt atccagttga atattattca ctcggaaatt
    24661 ttgcggtatc gagagatatt tgcctttaat taccccatag ccgattaaca tcccggcgct
    24721 attaatcatt tcaatattat caatcacgaa attgtcacaa ccgtaaatag cgactgtcgc
    24781 gttatcaatg cccgctttct tactgaaatc cggcgtgata ttgcgggcat tgatattacg
    24841 aataacaaaa tgtttaccat tttcaacatg gatcaactgc cgacaatccg atcccgtgat
    24901 attcgccacg acaaagtttt tcactgcctg gtcttccggg tagttgttat cataagtgct
    24961 tcccgcaagg cctatgccga tgccccagtt gattttgccg ttggtacagt tgatacgctc
    25021 gatgacatgg tcagatatca aaatatcact gtcgttaatt gccacgttcc attcaatggc
    25081 gtcgccctgt aagtcgctga acttacagtt ggtaatgttg gcaccgataa tctggttatg
    25141 aaatccctgg cgtaagatgg cgtaattagc gtggctgaca gtgaggttat cgatggtcag
    25201 gttgcgcatg acccgtttgt ttttgccgcc aatataaatc tgcattaccg ggccaaagcc
    25261 gctcatcgcc agccctttga tggtgcagtc agaaccacgc acatccaggg taatgttatg
    25321 cataccgccg ccctctcccc ctgtcacctg gctgccgtcc tgtaagacaa atcgccctct
    25381 gccgttgccg cgcaggcttc caaggatgtg taacgttttg ccgggaggga tgaagatgcc
    25441 ggtgttgata ttgtcacaaa cgaatccggc aggcacgacg accgtttgcc cttcgctgaa
    25501 ggcttgttta aatgaggcga tccagtcatg tgggttgtag tcgttaatgt taacgctttg
    25561 tcgggcggga agtgcgcgag cgaaaggggt atggaggaag gcaagcgccg agcttgccgt
    25621 caggaaggtg cgtcgggaga gtgttttaaa tggcatacgt tctcctctat aaagcctgca
    25681 gcaagctggc gagttctcga ttaatcacct gctggttaaa atcgtgttcg actttttcgc
    25741 acgcacgttt gacgaccgga gccagttcgt cggtatccag ttggctaaac gccgccagac
    25801 gttgtgccag tgcgcgagca tcgttctcag gcagcagcca gccggattta tcggcctcca
    25861 ccagttcagg aattccgcta tgcagagtag aaaccaccgg aataccgacc gccatcgctt
    25921 ccattagcgc caccggaatg ccttccatat cgccatccgc gcccgttacc gatggcaaca
    25981 ggaagacatc cgcgtcgtcg agcatctcct tcacttcatg gctcggttta aagcccggca
    26041 tctctaccac atcttccagt tgatattgtt cgatgatggt gcgcaggcgt cgttcccaca
    26101 ggccaatgcc gaggatgcga tagcgaaatg ccacgccctg ctctttcaac tgacggcagg
    26161 cttcaatcgc cacatgcagg ccttttttct cggttaagcg tgcgacagat atgatttcca
    26221 gcggcgttgc gggcgctttc accggacgcg ggctaaagcg cgtcatgtcc acgcccatac
    26281 gcgatacggc gattttttct ctggggcagc ccattttttg cagccttccg gcccacagat
    26341 cgcttatcgg taacatcagg tcgccacggc gaaacagttg ttgatattcg ggagtgtagt
    26401 ggttgagcac ttcccgactg gagatatcaa taccgtggaa gatggtggca attttgccgc
    26461 gaatgatacc cagttcgcgt agttttgctg cggttacccc cgccgggcca aaatgagcga
    26521 tgaacacatc ggcgcgaaac ggtgttgcga cctgaccaca aatggcagac aaaatcaggt
    26581 tccgcgactc ggcaccatag cgtttgaggt tgagcgcatg ccaggtattt ttacgatgaa
    26641 tgccacgtaa ggtctggctg gcgcggtggc gcagtttcgc tactttgccc gcaggttcgt
    26701 cctgtaacca gcgggttctg gcgacaaggt tgtatttcgt ccatgccgcg tgggtgttct
    26761 ctgtatcgcc tttttgcaaa gcgacaatct ccacttcaaa gcccatatca ataaacgcgg
    26821 taatttgatt gaggacgaag gtttccgacg acagcgggaa tttcaataag aagaagccga
    26881 ccttcatttc acctccccga tgcgctcaag cacagactgc accatctgca ttcccgtctg
    26941 acgttcgcga ctgacggctt cgttaaatcg agcgttcagc accggaagct ggcctaaggt
    27001 atccgcaacc atcgcttgca ggctgccgtc cagtaaatga cgaatatcaa tcgccatctc
    27061 tggcagtccc agctgctgca taatcccggc ggatttatgt tcatagttga tggcaatcgc
    27121 cggagtagca aagttcattg agataatggc ggagtgcagg cgcgtaccga cggtgagttc
    27181 acaggctccg agaattttgc ccatttccag atcgttgagt tcatccatca ctacgtggta
    27241 acgggcagga tcgctgatgt gctggcgcag attgagcgcc accatgcggt cgtctttgtt
    27301 atagctgtcg atacccgtac aggtggagag cgcaatcacc tgatagcctt catcgagaat
    27361 gcgattgacc accccggcaa aggctttttc atacgcttgt tgagtggtgc cgagacgttt
    27421 gtcgaacggt gccagttcgc gcagggtaat tgccactgtt ttctgttgtg cggcaacgtc
    27481 cagccagtgt tgaacggcat agctggcggt gaagtcttcg gtgtggtgat cgaccagcca
    27541 cgcggtatcg acgccatgtt ccacttttgc ggtggtgata tttctgcgtt tcatcaaatc
    27601 aaggctgacc gattcgcgca ggatcagcgc gtcgcagtga ccaaagacat agttcgccag
    27661 ttggttaaat tgctcatcct ggaaggggcc gacgctgtga ccaatcataa acagcggctt
    27721 tttcgccata aatgtgcaaa gcgcatgttc aaactgcggc acgccgtaga gatcgacaaa
    27781 aaacgatccg ccgacctgaa taatggcgtc gtaacctgac agcaggcgca cgaagtcggt
    27841 gaatccctgg gcgatggcga tattgcgcag cttgccagtg tcggtgacgc gtgagagcaa
    27901 tacctgatgc tggtagcggc gacggaggac ttttttaatg cgcccgacaa cgcccgccgc
    27961 gctattgtgt tgtgtcatct gcaggaacag cggatcgccc attaccgggc ggttgagcag
    28021 ccaggaagaa ctgaccggat agcggctcat cacgtccact tcggtatgtg gattgaggat
    28081 gttgatggca tcaagtaagc cgcgcaagat ggcgctgtcg ccacgattgc cgcaagtgtg
    28141 gttgcccaga ataagtaatt tcattttttc ctcataaatt tgatgccagg tgaggctgtg
    28201 tttgttgtgt tcgttgccgt tttttgcctg atgcgacgct ggcgcgtctt atcaggccta
    28261 caggtcccga gcacagaacc gtaggacgga taaggcgttt tacgccgcat ccggcaaccg
    28321 ttgtcggaac cgaaaacagc aattcacccc gcccgtaaaa gcattttcat tttttcgctg
    28381 cgacaaaact gacgcttcac ttccaccacc agcggatggc gtgacagcac aatcatcacc
    28441 acaaacgcca gcacgcccgc ggctatttgc accgccagca gcatccccag cgccagttgc
    28501 cctttcagca caatgcccag cgcataactg accaccagcg tcggcaacga gagataaaac
    28561 ggcagccaca aactcaggat gtactggcga taactggaac caagaaccgg tttaatcatc
    28621 acgaagtaac tcagaatggt gttgataatc tgcaccagca ggaaaccgag cgtgacgccg
    28681 attgcgcccg ccatctgccc accaataaca atcgccggaa taaacagaaa tgttttgaat
    28741 acgttgaatt taaagctgat atcgacccgc gctttcgcca tcagcagcga accaatcggg
    28801 ttacccacgg agcgcagcag acccacaata cacagcaatt gcagcaccgg aataatgctg
    28861 ttccactttt cgccgaatac cagcggtacg aagttattcg acaccaccat cagccccagc
    28921 agcgccggaa agttgataat ccccactatc gacagcagct tgtagaagtt aacacgcagc
    28981 ttttcggtgt cgtcctgaat tttggcaaac gccggaaaca acacgcgggt gatgattggg
    29041 ttcagcttca tcggcggtac gacggccacg ttgtacgcca ggttgtatcc ccctgccacg
    29101 cccgcgccaa gaatacgcgc cagcaccagc gttgaaaggt tggtgttgag atagttgatg
    29161 atgctgtccg ccgtcagcca ggcaccaaaa cgtaagttcg gtgccacaga cgccagcgag
    29221 aagtgcagac cggggcaata aattttgcgt ccaaagtagc caaacagcag tgttctcacc
    29281 gcactattga ccagataacc gaggatcgcg gtcatcgcca gcggccagaa atgggcgcta
    29341 actaccgtaa aagtgaagcc cgccagcacc gcgctggttt cgatcatgcc gattttgttg
    29401 aactccagct ccttctgcat caacgcgcgg aactgttgcc cgtgggggat caccacaaac
    29461 gccagcgaca atgttttaat caacggtgcc aggtccgggt tattcagcac gtcgccgatg
    29521 acatcactca acaaaaacac cgccacacat accacaagcc ccagcccgac gttcagccag
    29581 tacagcgtgg tgagttcaag gtgactgatt tcttttcgct gaataatcga gttagcgata
    29641 ccgaagtcag aaagcgtatc tgccagcgcg ataatcacca gcgacacagt cagcaggccg
    29701 aactggtggt tgtcgataat ccgcgccagc acggtcatct gcaccagccc gaggccgatg
    29761 atgatcaccg tggcaatagc cgaccacttc gcgccgctga tggttttttc acgtaagctc
    29821 atatcaatat gccgctttgt taacgaagcc cttgaatacc gtcaggaaaa cgattttgat
    29881 atcgaaccag acgctccatt cgcggatgta ctcaaggtcg aactcgacgc gtttttccat
    29941 tttctccagc gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc cggtaatgcc
    30001 cggtttcact ttatggcgca gcatgtagcc ttcaatgagc tggcgatact gttcgttatg
    30061 cgctactgcg tgcggacgtg gaccgacaat cgacatcccc ccggtcagca cattgataaa
    30121 ctgcggcaat tcgtccagcg aggtgcggcg cagaaagttc cccactttag tgacgcgcgg
    30181 atcgttctgc gtcgcctggg tcaccacttt gtcgttctcc atcactttca tggaacggaa
    30241 cttccacact ttgatcggct tgccatccat accatagcga gtctggcgga aaataaccgg
    30301 ccctggtgaa ctgagtttca ccgccagcgc aatacagcac agcaccgggg agatcagcag
    30361 cagaataagc gtcgccagca caatgtcttc cgcacgtttt agcaggcggt taacccccga
    30421 aagcggcgtg tcatacagcg gcaccaccgg aacgccgttc atctcttcga gacgtgaatg
    30481 gagaatgttg aaggtaaaga cgtcggggat cagcagcacc gaacaggtgg tatccgccag
    30541 ttgatggacc agttttttca ctcgtgcgcc gtcgcacatt tgcatcgcga tatagacgtt
    30601 atgaatcttg cccgcttttg catcctcgac cagctgttgc aggttacccg cccagtcgtt
    30661 agaaacgccg cccggtttcg ggtcgtggta aacgcccacc acttcaaacc ctaaccacgg
    30721 ctgattacgg aaactctcca tcagcatttg tccggcggct aaatcccccg ctacggcgac
    30781 catccgcttg ttgtagccat gattacgcag ccagcccgcc ccaatgcgaa tacatgaacg
    30841 gcaaaccacc agtccgatgc tggtcagccc ataccacgcc agccagattt tcagttgcgt
    30901 gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta aggtccagtt
    30961 ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc gataaaaatc
    31021 ggtgatgccg cccagcatct ggaacaccac cagcgtaatc agcgccacca acaggtgcat
    31081 gtagaggaat gacagtccgc tgacttcgca aaccagccat agcccggcaa acatgatggt
    31141 gatatctgaa aagcgttgca ccatagagat taacgatgca ttggttttcg ctcgctcgcg
    31201 cttttttaga tttgtcatcg ttgttcctgt tattagcccc ttacccgcag tggggtaagg
    31261 gaagatccga cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg cttccatcag
    31321 cggcacatca ccgcgcgatt ccacattcag gcgcaccacc ggttcggtat tggaggtgcg
    31381 caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcgctgcgat ccaccgccag
    31441 cgcctcacgg ctaaaatgct gttccacgcg gttaatcgcc tcaacgggtt gcgccagttt
    31501 gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca gttcgcccag
    31561 cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca tgccgctgtc
    31621 gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt agatggcgtc
    31681 ttccttgcgc atccgttctt taataaaggc gtgtccggtt ttcgacatta ccggcgtgcc
    31741 gcctgcagca gtcaccacat caatagtgtt ccaagagaga cgcggatcat ggatgatctt
    31801 cgcgccggga tttttttcga ggaacgcttc tgccagcagg ccgacaatgt agtagccctc
    31861 gataaactgc cctttttcgt caaacagaaa acagcggtca aaatcgccat caaaggcaat
    31921 gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg tcgtcgcgac attccggcag
    31981 cagcgggtta ggaataccgt tggggaaatt gccgtccggt gtgttgtgta ctttgattaa
    32041 ttccacgggc gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca ccggacccgc
    32101 tgcgccattc ccggagttga tcaccagctt gagcggcgtg agatttttga cattgatata
    32161 accgaacagg tgatcaacgt aagcgtcacg caggttgatt tgctgatagc gaccgcgttt
    32221 ggtttcatcg acgggaggaa agtcgttggc ttcagccaga cgctggacgt cgcgcagtcc
    32281 ggtatcaccg ctgatcgtgc gagccccctc gcgcaccagc ttcatgccgt tgtaatccat
    32341 cggattatgg ctggcagtaa cttcaatgcc gccatccacg ccgagatgga acgtggcgaa
    32401 atagatctct tcggtgccgg acataccgat atccagcaca tcgacgcccg catcctgtaa
    32461 accttttgcc agcgccagtt ttaaggtttc gctggtgaga cggacatcac cgcctaacac
    32521 aatggttttc ggtttgagaa attcgccata ggcgcgacca atgcgccagg cgatatcttc
    32581 attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg ttaatttttt
    32641 catgtcgtta ccctttttca ggcaatagtt ggccctgacc gaaacggcca attttatttt
    32701 tgtcgttatt tacaccgcgg tttcgcattc attgcctgat gcgacgctgc cgcgtcttat
    32761 caggcctaca aaaccgaacc gtaggtcgga taaggcgttc acgccgcatc cgacaaacag
    32821 cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg aagcgcacca catcatcctc
    32881 ttcgagataa gagccggagc gcacttcaat taaatcgagc ggaattttcc ccgggttttc
    32941 caggcagtgc gtcgccccca gcggaatata aatggactcg ttttcaccaa gcagtttgat
    33001 atcaccatca atggtgactt ttgccgttcc cgcgaccact acccagtgtt ccgcgcggtg
    33061 atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt tcacctggta
    33121 gcggtcgccc gcgtcgatag agtcatattt gccccacgga cgatacactt cgcgatgcac
    33181 ccgatgctca tggcgaccat cggctttgat ctgctcgacc acttttttca catcctgtac
    33241 cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc accactacca gatctttcac
    33301 cccgacggtg gtgaccaggc ctgattcggc atacacatag ctgttttcag ttttgtgatt
    33361 aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct cccataatga
    33421 agaccaggaa ccaacatcgc tccagcccgc atccatcggc accacaacag catctgccgt
    33481 aggttccatg accgcgtaat ccaccgactc ttccgggcag gcgagaaacg cttcttcatc
    33541 cacacgaata aaatcgagat ccggatcgac ggcgctcatc gctttttcgc aggcatcgag
    33601 gatatccggg cgatattttt tcagttcttc gagatagcgt ccggcgcgga acaggaacat
    33661 gccgctgttc cagtaatatt cgccgcttgc tacataggcc tgggcggttt ccagattcgg
    33721 tttttcgaca aactgcgcca cttcaaaggc caccgtatcc tgctcaccca cagacacttc
    33781 accgcgacga atatagccat aaccggtttc tggtagatcc ggcacaatgc caaaggtcac
    33841 cagcttgccc gcttcggcat aaggcatggc attacgcacg gcggcacgga acgcgtcttc
    33901 atcggcaatc acatgatccg ccgccaatac cagcattaac gggtcgctct ccgggctatg
    33961 acgttttgcc gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc ctgccggttc
    34021 gagaataatg ttctcggtga gtttgttcag ttgacgcagc tgttccgcga caataaagcg
    34081 gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc acgccgttca ggcggcagat
    34141 ggtggtttgc agcatggtga gatcgccttt caggcataaa aactgcttgg ggtaaagtac
    34201 gcgggaaagc ggccataaac ggctaccgga gccacctgcc atcacaactg gatagagttt
    34261 cgactgcgcc ataattatcc ccgaatatca tttataaatt gacgtaacac gttctcttta
    34321 tcgagcgtgc gttcggcata ttcacgtgcc accgtgttat gtttgggcag caggagcgcc
    34381 tgacgaatcc ccgccaccag cgcctcgacc gattccggtt ctacgcaaac cgcaataccc
    34441 ggaaaggttt cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat caccgcgtta
    34501 ccgcctaccg ccagaatatt ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt
    34561 ttttgcacca ccagatggca atcgcccatc ttcagcagtg caggtaaagc gtcatacgat
    34621 tgcagcggga aaaattgcat gttgcgcagt ccacgctgct gcgccatttt ttccagccgc
    34681 gctttgccgc cgccctgccc gacaatggca aaaatcagcg gttcatcgcg caggagatcg
    34741 gcagcttcaa taacgttttc cagcccctgc ttttcaccaa tattgccgga gtaaagaatg
    34801 atttttttgt tatccggcag gccaagctgg ttacgaaggg catcaacatc ggcgtctgca
    34861 acatgctgaa aacgggcaat ttccgaccag ttggggaaga agatgacgtt atccgccgcc
    34921 acgccttttt cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac gttatcgacg
    34981 ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg ccactttgcc gcctttgcct
    35041 tttccggcaa ggcccagccc cagcatggcg tccacttcgt aatcctgaat atgcagcacg
    35101 gtacgcgcac cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc
    35161 actacgccaa taatgcgatc cggcttccag cgacgttgcg ccatcagtgg aaagaaactg
    35221 ctgacggcaa aactgccgag atgcaacaag cgtttcaggg tgctcggctg ttttggcacg
    35281 tacagcgggc agcgccacac cgtggcggcc ccttcttctc gtttgtagcg ccaggcggaa
    35341 tagttctcac ccacctgcca ttgcgggtag taaggcggtg cggtaatgac ccgcacctca
    35401 tgaccttgtg ccgccaacca ttccaccatc tcgccggtgt atttgccgat gccggttaac
    35461 tccggcgagt agttaatgcc gtagaccagt attttcataa tccgggtact ccggcacgct
    35521 tctcagcgag aaaataggct cggctgttag cgtgaacatc gttactggcg agcagcgcgt
    35581 ccggcgtcag ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc tcttcttccg
    35641 ctactctgaa gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg ccggagaagt
    35701 tatcgtcata aaaatgctgc cagacaccgt aaaactggcc tgctgttatc ggcagacgca
    35761 gccccagttc cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg tctttctgca
    35821 cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg gttggttctt ttgccaagca
    35881 gaaactcgcc gcgactgttc tcgacaataa agtcgagaga gacaagcggt gtggagcgca
    35941 ctacagtggc aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc ggtcttgatt
    36001 ctcaaggaac cactggtaag tgctggcaag ccccgcttcc agtgagattt cgtgatacca
    36061 gccaagctga tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat ccggtttgct
    36121 ggcatcaaaa accacccgac ctttgtaacc caccactttg gcgatggttt gcgccagctc
    36181 gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg gttgggtatt
    36241 ctccagccag acttcatgcg ccagctccat gacatgaatg ctcgccgccg ccatatcatc
    36301 gacgtgcagg aattcacgca tcggtgtacc gctgccccat accaccacgt ccggcgcatt
    36361 ctgtgccgtc gcctcgtgga agcgacgcag caatgctggt atcacatggg agttactcgg
    36421 gtggaagttg tcatgcggcc cgtacaggtt ggtcggcatg acagtgcggt aatcgcgtcc
    36481 atactggcgg ttgtaagatt cgcacagttt gatcccggcg attttggcaa tggcgtaagg
    36541 ctcgttggtc ggctccagcg tgccctgcaa taactcgctt tctgccatcg gctgttttgc
    36601 cagtttcggg tagatgcagg acgatccgag aaacatcagt ttgttcacgt cgttctgatg
    36661 cgcggcgtga atgatgttgc tctcaatcat catgttctgg tagatgaaat ccgccggata
    36721 ggtgttgttg gcaacaatgc cgcccacttt cgccgccgcc agatagacct ggtcaatgcg
    36781 ttcgctggca aagaaatcat gcacggcgcg gctgtccagc aggttcagtt catcgcgggt
    36841 acgtaatacc agctccacat caccgcgctg ttcgagctgc cgcttgatgg cagaaccgac
    36901 catcccacaa tggccagcga taaaaatgcg ttgtttactc atgcttatga ctccagcgcg
    36961 atcgccacgt cgtagccgtg agatttcagc agagagtgtt ttttcgccgc ttcgaggtca
    37021 ttagccacca tttcagacac catctctctg agggtgattt ccggtttcca gcccagtttt
    37081 tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt ctgccggacg gaagtaacgc
    37141 ggatcaacgg cgataatcac atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg
    37201 gaaaccacaa tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc
    37261 tgtgctgccg ccatttccac aaactgacgt acggagtact gaacgccggt cgcgataacg
    37321 aaatcttccg gctgttcctg ttgcagcatc atccactgca tttttacgta gtctttggcg
    37381 tggccccagt cacgcaggga atccatattg ccgaggtaca ggcacgactc cagcccctgg
    37441 gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg
    37501 gattcatggt tgaagagaat tccgttacaa gcgtacatgc cgtaggattc gcggtagtta
    37561 acggtgatcc agtaggcgta cagtttggcg accgcatacg gagatcgcgg gtagaacggc
    37621 gtggtttctt tctgcggaat ttcctgcacc agaccataca gttcagaggt ggaagcctga
    37681 tagaaacgag ttttcttttc cagaccgagg aagcggatcg cctccagcag gcgcagcgta
    37741 cccatcgcgt cgacgtcagc ggtatattct ggtgactcaa aagagaccgc aacgtggctc
    37801 attgcgccta ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta
    37861 gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc
    37921 tgataaatgt gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt aataccatgc
    37981 acctcgtaac ctttttccag cagaaactct gccaggtaag aaccgtcttg tccggttaca
    38041 ccggtgatga gagcgacttt tgacatgtct tattcctctg tatttttgaa tttattcagt
    38101 ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg caaaccacat ttgccggaag
    38161 cgatttaaaa acactgcttc gtgcacccac gacggtgccg tcgccgattg tgacgcctgg
    38221 ggcaacaaag acatcggttg ccagccagca tttctcgcca atcacaatag gcgtggcgtt
    38281 aatggtgaaa tgttgacttg catggtcgtg gctaccggtg cataaataac ttttttgcga
    38341 tatcaccgaa tgtgcgccaa tggttatttc accgagggta tataaattga cgtcatcgcc
    38401 aacccacgcg taatcaccta aggttaattt ccacggatag gtaattttta ctgacggacg
    38461 aataactacg ttttttccta tttttgctcc gaataaacgt aataaaaaag cccgccagcg
    38521 atacaatact tgtggcgacc aggcaaatat tgttgcctgt actgcccacc ataattgcac
    38581 tttaatagcg ttgccgcccc ggaacccttt cggcaccgag aaaccgctta aatcttgcat
    38641 tggttttcct tatattcaga ctttgttata taaggcttta gtcttacctg tcgtacgttg
    38701 gcgtaaatgc caggataatt ctgcccagaa gccaggtaca tgtaatatct gccgttggac
    38761 ttttttggcg tcagcac
//
LOCUS       DAJVQR010000030        37074 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVQR010000030 DAJVQR010000000
VERSION     DAJVQR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..590)
                     /gene="yhdJ"
                     /locus_tag="OQM52_001634"
     CDS             complement(<1..590)
                     /gene="yhdJ"
                     /locus_tag="OQM52_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1563526.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPT"
     gene            complement(676..972)
                     /gene="fis"
                     /locus_tag="OQM52_001635"
     CDS             complement(676..972)
                     /gene="fis"
                     /locus_tag="OQM52_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1563527.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(998..1963)
                     /gene="dusB"
                     /locus_tag="OQM52_001636"
     CDS             complement(998..1963)
                     /gene="dusB"
                     /locus_tag="OQM52_001636"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1563528.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2292..3173)
                     /gene="prmA"
                     /locus_tag="OQM52_001637"
     CDS             complement(2292..3173)
                     /gene="prmA"
                     /locus_tag="OQM52_001637"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1563529.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3185..4636)
                     /gene="panF"
                     /locus_tag="OQM52_001638"
     CDS             complement(3185..4636)
                     /gene="panF"
                     /locus_tag="OQM52_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1563530.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4626..4868)
                     /locus_tag="OQM52_001639"
     CDS             complement(4626..4868)
                     /locus_tag="OQM52_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1563531.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(4977..6326)
                     /gene="accC"
                     /locus_tag="OQM52_001640"
     CDS             complement(4977..6326)
                     /gene="accC"
                     /locus_tag="OQM52_001640"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1563532.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6337..6807)
                     /gene="accB"
                     /locus_tag="OQM52_001641"
                     /gene_synonym="fabE"
     CDS             complement(6337..6807)
                     /gene="accB"
                     /locus_tag="OQM52_001641"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1563533.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(7785..8759)
                     /locus_tag="OQM52_001642"
     CDS             complement(7785..8759)
                     /locus_tag="OQM52_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1563534.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            8911..10851
                     /gene="csrD"
                     /locus_tag="OQM52_001643"
     CDS             8911..10851
                     /gene="csrD"
                     /locus_tag="OQM52_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1563535.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11156..12199
                     /gene="mreB"
                     /locus_tag="OQM52_001644"
     CDS             11156..12199
                     /gene="mreB"
                     /locus_tag="OQM52_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1563536.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12265..13368
                     /gene="mreC"
                     /locus_tag="OQM52_001645"
     CDS             12265..13368
                     /gene="mreC"
                     /locus_tag="OQM52_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1563537.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13368..13856
                     /gene="mreD"
                     /locus_tag="OQM52_001646"
     CDS             13368..13856
                     /gene="mreD"
                     /locus_tag="OQM52_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1563538.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            13865..14458
                     /gene="yhdE"
                     /locus_tag="OQM52_001647"
     CDS             13865..14458
                     /gene="yhdE"
                     /locus_tag="OQM52_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1563539.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14448..15917
                     /gene="rng"
                     /locus_tag="OQM52_001648"
     CDS             14448..15917
                     /gene="rng"
                     /locus_tag="OQM52_001648"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1563540.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            15985..19785
                     /gene="yhdP"
                     /locus_tag="OQM52_001649"
     CDS             15985..19785
                     /gene="yhdP"
                     /locus_tag="OQM52_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1563541.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            19941..21386
                     /gene="tldD"
                     /locus_tag="OQM52_001650"
     CDS             19941..21386
                     /gene="tldD"
                     /locus_tag="OQM52_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1563542.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21514..22443)
                     /gene="aaeR"
                     /locus_tag="OQM52_001651"
     CDS             complement(21514..22443)
                     /gene="aaeR"
                     /locus_tag="OQM52_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1563543.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22626..22829
                     /locus_tag="OQM52_001652"
     CDS             22626..22829
                     /locus_tag="OQM52_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1563544.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            22837..23769
                     /gene="aaeA"
                     /locus_tag="OQM52_001653"
     CDS             22837..23769
                     /gene="aaeA"
                     /locus_tag="OQM52_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1563545.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            23775..25742
                     /gene="aaeB"
                     /locus_tag="OQM52_001654"
     CDS             23775..25742
                     /gene="aaeB"
                     /locus_tag="OQM52_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1563546.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            25834..26106
                     /locus_tag="OQM52_001655"
     CDS             25834..26106
                     /locus_tag="OQM52_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1563547.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26162..26425)
                     /gene="yhcN"
                     /locus_tag="OQM52_001656"
     CDS             complement(26162..26425)
                     /gene="yhcN"
                     /locus_tag="OQM52_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1563548.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(26790..27260)
                     /gene="argR"
                     /locus_tag="OQM52_001657"
     CDS             complement(26790..27260)
                     /gene="argR"
                     /locus_tag="OQM52_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1563549.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27695..28633
                     /gene="mdh"
                     /locus_tag="OQM52_001658"
     CDS             27695..28633
                     /gene="mdh"
                     /locus_tag="OQM52_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1563550.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(28695..29762)
                     /gene="degS"
                     /locus_tag="OQM52_001659"
     CDS             complement(28695..29762)
                     /gene="degS"
                     /locus_tag="OQM52_001659"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1563551.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(29852..31199)
                     /gene="degQ"
                     /locus_tag="OQM52_001660"
                     /pseudo
     CDS             complement(29852..31199)
                     /gene="degQ"
                     /locus_tag="OQM52_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31353..31751)
                     /locus_tag="OQM52_001661"
     CDS             complement(31353..31751)
                     /locus_tag="OQM52_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1563552.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            31945..33072
                     /gene="zapE"
                     /locus_tag="OQM52_001662"
     CDS             31945..33072
                     /gene="zapE"
                     /locus_tag="OQM52_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1563553.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33291..33719
                     /gene="rplM"
                     /locus_tag="OQM52_001663"
     CDS             33291..33719
                     /gene="rplM"
                     /locus_tag="OQM52_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1563554.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            33735..34127
                     /gene="rpsI"
                     /locus_tag="OQM52_001664"
     CDS             33735..34127
                     /gene="rpsI"
                     /locus_tag="OQM52_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1563555.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34522..35160
                     /gene="sspA"
                     /locus_tag="OQM52_001665"
     CDS             34522..35160
                     /gene="sspA"
                     /locus_tag="OQM52_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1563556.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35166..35663
                     /gene="sspB"
                     /locus_tag="OQM52_001666"
     CDS             35166..35663
                     /gene="sspB"
                     /locus_tag="OQM52_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1563557.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(35706..37073)
                     /gene="dcuC"
                     /locus_tag="OQM52_001667"
     CDS             complement(35706..37073)
                     /gene="dcuC"
                     /locus_tag="OQM52_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1563558.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         8971 a         9288 c         9776 g         9039 t
ORIGIN      
        1 gtcgggtggt tctcatattc atccatcaaa taacgcacgc gcggaaaatc ccagacgtta
       61 cccggtactt tttgatgatt gtatggctgt ggaggatttt tgcgataatc gatcaacgcg
      121 cgctgcgatc cggttttggc ttctaccaga atagcatcac cgttgaatgt gtagttcttt
      181 gcgtctttca ccatcatcag gatgggttcg tacatggagc cgtagtgttt tttcgcctgt
      241 actccagaac tgtcatatga ccagacgatg cgacttttga tggtaaaaag cttgcggcac
      301 tggagatcga taaagggcat gttttccgta ctgttcataa tgtacatgct gccctgcttt
      361 ttcagaacgc ggtggcactc tgcaatcact tcaaacagcc agtcgataaa cagatcttct
      421 ttccaggctt cgatcagacc atcaaaattt ttaccgatgt tatacggtgg gtcggcaaag
      481 agcagatcga cgctttcagt aggtagcttt ttaagttcgg caaaggcatc accgtgaata
      541 atggtcttag cttcattacc aaaccgggtc ggttcacatc ctgttctcat ggtcactccc
      601 tttgtgacac ctataaaaaa ggcgcttccc catgccgagt agcgcctttt taatcaagca
      661 tttagctaac ctgaattagt tcatgccgta ttttttcaat tttttacgca gcgtaccacg
      721 gttgatgccc atcatcagcg cagcacgggt ctggttacca cgggtgtatt gcatcaccat
      781 gtccaacagg ggctgttcta cttcagccag taccagctca tagaggtcat tcacatcctg
      841 accattcagt tgagcaaaat agttcttcag tgcctgttta accgagtcac gcaggggttt
      901 ttgggttacc tgatcctgag agttaacggt agaaacggtc agtacgtcag aatttacgcg
      961 ttgttcgaac atagttctgt cagctcttta tttctgttta cgcaaaattt tcgaagtatg
     1021 cctccaacgc ctccagctgt tcgctggcat cctcaatggc gttgaatgtg cgccgaaact
     1081 ggtcatttgg agcgtgttcc tggagatacc aggaaacgtg tttacgtgca attcggtacc
     1141 cttttgccgg accataaaag tcatgcagtt cccgaacgtg cgcgcaaagc aagcgcttaa
     1201 cctctgccaa aggcagtggg ggcagcaact ccccagtgtc cagataatgc tggatttccc
     1261 gaaagatcca gggtcttccc tgagctgcgc ggcctatcat cagggcatcc gcccctgtat
     1321 agtcgagcac agctctggct ttaagcgggt cagtaatgtc gccattcgcg ataaccggaa
     1381 tggaaacttt ctgcttaact gcccgaatac tgtcgtactc agcttctcca ttgaacaaac
     1441 aggcgcgtgt acggccatga atggtcagag cctgaatgcc acagtcttca gccagttggg
     1501 caatctcttc gcagttacgg tgttccggtg cccagccggt gcgaatcttc agggtaacag
     1561 gaacgtccac tgcattgacg acctcggtaa ggatcgattt aacgacatcc gggtactgca
     1621 agagggctga acctgcgagc ttgcgattca cttttttagc cgggcaaccc atattgatat
     1681 caataatctg ggcaccgctt tccacgttaa tacgtgctgc atctgccatt tctttcggag
     1741 cgctaccagc aatttgcacg gtgcgaatac cgggttcatc aatgtgcacc atccgtaaac
     1801 gagatttgtc gctttcccaa acctgtgggt tagaagacat catctcggat actgtcaatc
     1861 cggctcccat ctcgtagcac aacgtccgaa aaggtctgtc tgtaatgcca gccatgggcg
     1921 ctgcgatcag gcgatttctg agctgatatt gtccgatgcg catgagttaa gaaatgacca
     1981 tactgtgact gcaaggcggc gtatattacg cattttttgc acgagatgaa aggccaaact
     2041 ttgaccaatc ctctgagatg gatcaaagaa ttgcatttaa aatgagcgtg gtgcgataat
     2101 tactcataaa aatcatcata ttagaaaata gtgactaaaa tttacactca aagaaatttg
     2161 agtaagttct caatttttct ttatgaatga aaattttggc acgcaaattt gcgtaaataa
     2221 tcggcaaatt tacgtgcctt ttgtgagctt gctcgcactt cgccccgcgt caccctacgg
     2281 cgatgcgaag gtcaattctt acgaccggta atacggcacc actcttcttt ttccacgacc
     2341 gggtccagtg cgaagctatc ggcataagct tcacaaacgc tctctgcctg gcttgccaga
     2401 ataccggaaa ggcccagcaa accgcctgaa actggcagga cgctgattaa cggtgccagt
     2461 tcacgtaatg ggcctgcaag gatgttagcg accaccacgt cggctttcat ttcttctggc
     2521 tgatctttcg gtaagtagag ttccagacgg tcagaaacgc cattacgttc ggcgttatcg
     2581 cggctggcct gaatcgcctg cggatcgata tcaataccaa tggcttttgc tgcacccagt
     2641 ttcagcgccg cgatcgccag aatgccggaa ccacagccaa agtcgatgac tgttttaccg
     2701 gttaaatcga ggctgtcgag ccattgcagg cacagagagg tggttggatg ggtacccgta
     2761 ccaaacgcca gccctggatc taacatcacg ttgacggcgt tttcgtccgg cacatcacgc
     2821 cagctagggc agatccacag tcgttcacca aagcgcatcg ggtggaaatt atccatccat
     2881 tcgcgctccc agtctttatc ttctagttgt tcgattttat gcgcgaagcc tgcgccgagc
     2941 agcggatggt tttccagaat cgccaccacg tcgttcatat cggtttcagc gtcgaacaga
     3001 ccaatcacat cggtgtcgcc ccacaggcgc gtttcgcccg gcagcggctc aaatactggc
     3061 gtatcgtggg tatcctgaaa agtgatagaa acggcacccg cttccatcag cgcatcgcta
     3121 agatcttccg cgttcgcgcc ggtggtgttc agtttcagtt ggatccaagg catggcaaaa
     3181 ctctttattt atcagtagtc aaaacggtag cttgcgggac ggatgtaccg aaacggtttc
     3241 cgaccaggaa agccagcaaa cttagtagta acgagggcac gatagggtgg aagcccaggt
     3301 actgaatatt cagcgtcgcg agtacggcat acagcacgcc gccaacgatc atcgcactta
     3361 gcgcgccttt ggcgttggcg cgttcccagt aaagacccag caccagcggc cacaggaaaa
     3421 cggcttccag cccaccgaag gccagcaaat tcagccagat gatcatttct ggcggcttcc
     3481 aggcggcaag cagcagcaac gcgccgagaa ctaacgtaat taccgccgac atccgcttca
     3541 gacgcgtctc gttttgcatt tgatccggac ggatattcag atagagatct ttaatgatcg
     3601 tagcggaact ttgcagcagt tgggcgttaa ttgtcgacat gatcgcagcc ataggtgcag
     3661 ccagaaagat cccggcagca aaaggtggca gcacttttac cattaacgtc gggatcacca
     3721 gatctggtac ggtgagatcg gggatcactg cacgacctaa cgctccggcc aggtgcatac
     3781 cgaacatcag gattgcgact ataatcgtac cgatgatgat cccccggtgt actgctttgc
     3841 tgtctttata agagatacag cgcaccgcag tatgcggcag gccaatcacg ccaaagcaca
     3901 ccagtaccca gaacgacgtc ataaaggcag gcgacagaat atcgtcagcg ccttgtggcg
     3961 taaccagttg cggatcgatg gtttgcaagg tctgtactgc gttgcttaag ccgccagcgg
     4021 catgtaccac gccaataagc agcacaacgg tgccaatcag catcacaagc ccttgcatgg
     4081 tgtcgttcag cacgctggcg cgaaagccac caaaggcggt atataacgca atgctgatac
     4141 caaaaatcag cagcccggtt tcataaggaa tacccgccgc ggtttccagc aggcgcgcac
     4201 cgccgataaa ctgcacggtc atcgcaccaa cgaacgcaac cagcaaactc aaactcgcca
     4261 gccacaccag aagacgactc tggtagcggg caaacagcat atcgttcagc gtcactgcat
     4321 tgtagcggcg cgcaagaatc gcaaacttct tgccgagaat accgagtgaa agccagacag
     4381 caggaagctg aatcatcgcc agcaataccc agcccagccc gtatttataa gcagctcctg
     4441 gcccgccgat aaacgaactg gcactgatat aggtcgcggt gagagtcatc gccagcacaa
     4501 taccgcccat agagcggctg ccgaggaaat actcattaag gaaggtgccg gtgctccgtt
     4561 tacgcatcgc ataaaccgag ataccgaaca ccaccaccag ataggcgacc agcggtagaa
     4621 ttacttcaag ctgcatcgtc atcctccagt gggatatcgc gatagataaa tttcaccatc
     4681 gcccagcaca gtccaataaa cagtagcggc gtcaggatgc aggccatctc aaaccagcgc
     4741 ggaaagccgg taaaaccggg ggcaacgcca ggtaagtaag cgtctactaa ccaaactgcc
     4801 agatacaaaa gggtcagccc cagcgcccag cgggcctctt tatgggcctg aacaaaacga
     4861 gtgtccattt tttgtccctg atgggtgaag aaagcgagga ttgtacctta tgggggttgt
     4921 cgatccccag taataaaaaa ggccggaaaa tccggccttt tgacgctttt gcagtcttat
     4981 ttttcctgaa gaccgagttt tttctccaga tagtggatgt tagtgccacc atgctggaag
     5041 ttctcgtcat tcatgatgcg gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc
     5101 agctcctgca gcgcattctt catgcgggca atcgccacgt cacggttttc accgtagcaa
     5161 atcagcttac cgatcattga gtcatagtac ggcggtacgg tgtagcccgc gtagatatga
     5221 gactcccaac gtacgccaaa accgccaggt gcgtggaaac gggtgatttt gcctgggctt
     5281 ggcaggaagg tgttcggatc ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg
     5341 tgaacttctt cttgcttgat cgacagcggt tgaccggcag cgatacgcag ctgttctttg
     5401 atcaggtcaa cgccggtgat catttctgta accgggtgtt ctacctgaat acgggtgttc
     5461 atttcgatga aatagaactc gccgttttcg aacaggaact cgaaagtacc tgcaccgcga
     5521 tagccgatat caacacacgc tttagcgcaa cgttcgccga tgtagcgacg cagttccggg
     5581 gtaatgcccg gtgctggtgc ttcttcgacc actttctggt ggcggcgctg catggagcag
     5641 tcacgttccg ccagatagat agcgttgccc tgaccgtcag ccagtacctg aatctcgacg
     5701 tggcgaggat tctccaggta tttctccatg taaaccatat cgttgctgaa agcagctttc
     5761 gcttccgcac gggtcatgga gatggattgt gccagttcag cgtcgccgcg cactacgcgc
     5821 ataccgcgac cgccgccgcc gccggaggct ttgataatca ccggataacc aatgcgttta
     5881 gcaatggcgc ggtttttatc catatcgtcg cccagcgggc cgtcagaacc aggtacgcaa
     5941 ggaacgcccg cttttttcat cgccgcgatt gcggatactt tgtcgcccat caggcgaatg
     6001 gtttctgctt tcgggccgat gaagataaag ccggagcgtt caacctgctc ggcaaagttg
     6061 gcgttctcgg agaggaagcc gtaacccgga tggattgcta ctgcgccggt gatttcagcg
     6121 gcgctgatga ttgccgggat gttcagataa ctttttactg acggagcagg gccaatacag
     6181 accgtttcat ctgccagtaa tacgtgtttt agatcgcgat ccgtgctgga gtgcacagcg
     6241 acagtcttga tgcccagttc tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg
     6301 ttggcaataa caattttatc cagcatgttc gcctcgttac tcgatgacga ccagcggctc
     6361 gtcaaattct accggttgtc cactttcgac cagaattgct ttcacggtac cggatttgtc
     6421 cgcttcgatc tggttcatca ttttcatggc ttcaacgatg cacagggtat cgcccacgtt
     6481 gactttctga cccacttcga tgaacgcttt tgcgtccggg cttggggtgc ggtagaaagt
     6541 accaaccatc ggggaacgta cgatgtgacc actgatttcc gctgctgctg gcgcttccat
     6601 ggaaggaacg gtcgccggag cggctgcgtt agattgagct ggctgctgca tcattggtgc
     6661 agcgtaagct tgttgcatca cagggaaact tgcggcagga gctgcacggc taatgcgtac
     6721 tgactcttcg ccttcagaaa tttccagttc ggagatgcct gattcttcaa ccagctcgat
     6781 cagtttttta atcttacgaa tatccatgag tgggttccgt actctttgtt tagtgtgatt
     6841 gtgacaggcg tttcaccgcc gtctgtaaag cgtatgcccc gacagctgta tgcatagcga
     6901 taaattccag caggccggga cgccggtcta ttttgcccca gagtgtcaat gttagacttg
     6961 acggacattg tgcagcgatc tgctgctatc ctccggcaaa aaacaaaata taccttcgtt
     7021 gcgccaatgt caccttttcc gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat
     7081 tataacgatt tcgtagcaat tggcagctaa atactggtct tatcagggaa tataatcaac
     7141 agctaacatg taaataacct tcaacaccgt gtaatttgca acaagccgca caattcacga
     7201 aattagcgcc atcatcgacg gaacttctta taacgtaagg tgaaaagctg aaaagccagc
     7261 cctgcgaaga tgaggggctg caatgagata atcttcacag accacaaata atgtagggat
     7321 gccaggatcg cgacaagata gacgacatta cgcagcggtt gctggggatt acccaatttt
     7381 cactgcatcg tctggataga tgtgaatgct aaagcacgca aaatcaacca actgacaatt
     7441 ctcagcatta aataggttcg agtggttaat ttgtagtcaa ataaaatcag attattcacc
     7501 ccatggtacc agaaatcagt aacaggttaa gcgtagtgtc gcccaggcaa agcaccataa
     7561 acctaacagg cgacggaggc gtatcaataa tggctgttta gaggagtatc aatgcgcatc
     7621 aatttgtggt cagtcgaaat catgactttc cagtttggat gatgttgtac aacttcctga
     7681 ccaaggtgtt cgacgaaagc tgtccggcta accgcttttc ccgttaaccg attgaataat
     7741 gatgtcaaaa tgagttccct ctcttattat tccttgctaa atggttagtt aaccttcacc
     7801 agcgtgcgac cctggatctg gttattaatg atggcctcgg caaagttcgg tgcctctgac
     7861 agagatatct cttttgccgc ctgggtatag aatgattccg gtaaatcggc gaccagtcgc
     7921 tgccaggctt gtgcgcggcg ttctggtggc gtcattactg aatccacccc ttgcaaacgg
     7981 acattacgca gaataaatgg catgaccgtg gttggcagag taaaaccacc cgccagacca
     8041 caggccgcca cgcagccgcc gtaattcatt tgcgccagca cttttgccag cactttgtcg
     8101 ccaacagtgt caattgcccc agcccagact tgtttttcca gaggacggga ttcggcaaac
     8161 tcatcacgag ggagaatacc gctggcacct aaacttttca gatattcatg ggtactttcg
     8221 cgaccggaaa cggcaacgac ctgataaccc aacttatgca gcagcgccac ggcggtactg
     8281 ccgacgccac cactggcacc cgtcacgaca atctcaccgt cctgcgggcg aacaccggca
     8341 tcttccagcg ccatcacaca cagcatggcg gtaaaaccgg cagtaccgat aatcattgct
     8401 ttacgcgcgt ccagcccttg cggcatggca accagccagt cacctttcac tcgcgcctgc
     8461 tccgccagcc caccccagtg gttttcacca acgccccagc cagtgagtaa cacctcctga
     8521 ccggcatgaa aacgcggatc ttcgctggtg cgtacagttc cggcaaaatc gatcccagga
     8581 atcatcggaa aattacggat gatttttccc ttaccggtaa tcgccagcgc atctttatag
     8641 ttcaggctcg accagtgaac atcgaccgtg acatcgccct ccggcaggcg acgttcgtcc
     8701 agagtctgta ctgatgcgag agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata
     8761 agtggtcctc atgtgcatga tggattggaa aataatttct gaagactata ctcgctaatg
     8821 gaaaagcaaa gccgatgaag cgcaataaat tgcgagataa atctgatttg ctagtatgcc
     8881 cgcttcctca ctatcggagt taacacaagg atgagattaa cgacgaaatt ttcggccttt
     8941 gttacgctgc tcaccgggtt aacaattttt gtgactttgc tgggctgttc gctaagtttc
     9001 tacaacgcca ttcagtataa gtttagtcat cgcgttcagg cggtggcgac ggcgattgat
     9061 acccaccttg tgtcgaatga cttcagcgca ttaaggccac aaattaccga attaatggtg
     9121 tcagcagata tcgttcgtgt agacctgctg catggtgata aacaggttta taccctggcc
     9181 agaaatggta gttatcgtcc ggttggcacc agcgatctgt ttcgtgaact gagcgttccg
     9241 ttgataaagc atccggggat gtcgttgcgt ctggtttatc aggatccgat gggcaactat
     9301 ttccattcgt tgatgaccac cgcgccgctc acgggggcga ttggctttat cattcttatg
     9361 ctctttctgg cggtacgctg gttacaacgg caacttgccg ggcaagaatt gctggaaacc
     9421 cgggctactc gtatcttaaa cggtgagcgt ggctctaatg tgttgggaac catctatgaa
     9481 tggccgccca gaaccagcag tgcgctggat acgctgcttc gtgaaattca gaacgcacgc
     9541 gaacaacaca gccgtcttga tacgctgatc cgctcttatg ccgcacagga cgtgaaaacc
     9601 ggcctcaata accgactctt tttcgataat cagttagcaa cgttactgga agatcaggag
     9661 aaagtaggta cccacgggat cgtgatgatg attcgtctgc cggatttcaa tatgttgagc
     9721 gatacctggg ggcacagcca ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg
     9781 acatttatga tgcgctaccc tggcgcactg ctggcgcgtt accaccgcag tgattttgct
     9841 gcgctgttac cgcaccggac gttaaaagag gcagagagca tagccagtca gttaatcaaa
     9901 gccgtcgata ccttgccgaa caataaaatg ctcgatcgcg acgatatgat ccacattggt
     9961 atctgtgcct ggcgtagtgg tcaggatacc gagcaggtaa tggaacatgc agagtctgcc
    10021 acgcgtaatg cgggattgca gggtggcaat agctgggcta tttatgatga ctcgttgcct
    10081 gaaaaaggac gcggtaatgt tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc
    10141 ggcccgcgcc tttatcaaaa accggcggtt actcgcgaag gtcaggttca tcatcgcgaa
    10201 ctcatgtgcc gcatcttcga tggtaatgaa gaggttagct cggcggagta tatgccgatg
    10261 gtcttgcagt ttggcttatc ggaagagtat gaccgtctgc aaatcagccg tcttattcca
    10321 ctattgcgtt actggccaga ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt
    10381 cgtccgcgtt ttcagcgttg gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga
    10441 aaacgcataa ttattgaact tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa
    10501 cctgttattc gtttagtgaa tgctttaggg gtacgggtag ccgtcaacca ggctggtttg
    10561 acgctggtaa gcaccagttg gatcaaagaa cttaatgttg agttactcaa gctccatccg
    10621 gggctggtca ggaacattga gaagcgaacg gagaaccagc tgctggttca aagcctggtg
    10681 gaagcctgct ccgggaccag cacccacgtt tacgccaccg gcgtgcgttc gcgaagcgag
    10741 tggcagaccc tgattcagcg cggtgttaca ggcgggcaag gggatttttt cgcgtcctca
    10801 cagccacttg atactaacgt gaaaaaatat tcacaaagat actcggttta acctgccgtt
    10861 taatccgttt tcacgtagaa taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc
    10921 gccagattgt tgcagcacat atgcagatga atgaccttac gcggttgcaa acaggcgagg
    10981 aatgctgctg atgcattaag cctttctgga ctcaggcaga gatttgtaac aaaggaaacg
    11041 aactgcacta attttcaccg tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt
    11101 tggtaaagta agcggatttt cttttccgcc ccagctttca ggattatccc ttagtatgtt
    11161 gaaaaaattt cgtggcatgt tttccaatga cttgtccatt gacctgggta ctgcgaatac
    11221 cctcatttat gtaaaaggac aaggcatcgt attgaatgag ccttccgtgg tggccattcg
    11281 tcaggatcgt gccggttcac cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat
    11341 gctgggccgt acgccgggca atattgctgc cattcgccca atgaaagacg gcgttatcgc
    11401 cgacttcttc gtgactgaaa aaatgctcca gcacttcatc aaacaagtgc acagcaacag
    11461 ctttatgcgt ccaagcccgc gcgttctggt ttgtgtaccg gttggcgcga cccaggttga
    11521 acgccgcgca attcgtgaat ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga
    11581 agaaccgatg gctgccgcaa ttggtgctgg cctgccggtt tctgaagcga ccggttctat
    11641 ggtggttgat atcggtggtg gtaccactga agttgctgtt atctccttga acggcgtggt
    11701 ttactcctct tccgtgcgca ttggtggtga tcgtttcgac gaagctatca tcaactatgt
    11761 gcgtcgtaat tacggttctc tgatcggtga agccaccgca gaacgtatca agcacgaaat
    11821 cggttcggct tacccgggcg atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc
    11881 agaaggtgtt ccacgcggtt ttaccctgaa ctccaacgaa atcctcgaag cactgcagga
    11941 accgctgacc ggtattgtga gcgcggtaat ggttgcactg gaacagtgcc cgccggaact
    12001 ggcttccgac atctccgagc gcggcatggt gctcaccggt ggtggcgcac tgctgcgtaa
    12061 ccttgaccgt ttgttaatgg aagaaaccgg cattccagtc gttgttgctg aagacccgct
    12121 gacctgtgtg gcgcgcggtg gcggcaaagc gctggaaatg atcgacatgc acggcggcga
    12181 cctgttcagc gaagagtaat cggatgcagg caggggaagc gtctgtttac cctgcctggt
    12241 ctgatacgag aatacgcata acttatgaag ccaattttta gccgtggccc gtcgctacag
    12301 attcgcctta ttctggcggt gctggtggcg ctcggcatta ttattgccga cagccgcctg
    12361 gggacgttca gtcaaatccg tacttatatg gataccgccg tcagtccttt ctactttgtt
    12421 tccaatgctc ctcgtgaatt gctggatggc atatcgcaga cgctggcctc gcgtgaccaa
    12481 ctagaacttg aaaaccgggc gttacgtcag gaactgttgc tgaaaaacag tgaactgctg
    12541 atgcttggac aatacaaaca ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg
    12601 cgtcaggatg agcagaaaat ggtgactcag gttatctcca cggttaacga tccttatagc
    12661 gatcaagttg ttatcgataa aggtagcgtt aatggcgttt atgaaggcca gccggtcatc
    12721 agcgacaaag gtgttgttgg tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg
    12781 ctgatttgtg atgcgaccca cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta
    12841 attgcagccg gtaacggttg tacggatgat ttgcagcttg agcatctgcc agcgaatacg
    12901 gatattcgtg ttggtgatgt gctggtgacc tccggtctgg gcggtcgttt cccggaaggc
    12961 tatccggtcg cggttgtctc ttccgtaaaa ctcgataccc agcgcgctta tactgtgatc
    13021 caggcgcgtc cgactgcagg actgcaacgt ttgcgttatc tgctgctgct gtggggcgcg
    13081 gatcgtaacg gcgctaaccc gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt
    13141 ctgatgcaga tgatgccgca ggtattgcct tcgccagacg cgatggggcc aaagttacct
    13201 gaaccggcaa cggggatcgc tcagccgact ccgcagcaac cggcgacagg aaatgcagct
    13261 actgcgcctg ttgcgccgac acagcccgct gctaatcgct ctccacaaag ggctacgccg
    13321 ccgcaaagtg gtgctcaacc gcctgcgcgt gcgccgggag ggcaatagtg gcgagctatc
    13381 gtagccaggg acgctgggta atctggctct ctttcctcat tgcgctgttg ctgcaaatca
    13441 tgccctggcc ggataacctg attgttttcc ggccaaactg ggtgttactt atcttgttgt
    13501 attggatcct ggccttgccc catcgcgtaa atgtgggcac aggttttgtg atgggtgcca
    13561 tactggatct gatcagcggc tcgatgcttg gcgtacgcgt attggcgatg agcatcattg
    13621 cttacctggt ggcgctgaaa taccagcttt tccgcaacct cgcattatgg cagcaggcgc
    13681 tggtcgtcat gttgctttcg ctggtggtgg atattattgt tttctgggca gagtttttag
    13741 tgattaacgt ctctttcaga ccggaagtgt tctggagtag tgtagtcaat ggggtgctct
    13801 ggccgtggat tttcttgctg atgcgtaaag tccgtcagca gtttgcagtg caataaaggt
    13861 ttctatgact tctctgtatt tagcttccgg ttctccgcgt cgtcaggagt tacttgcgca
    13921 acttggcgtg acctttgaac gtattgttac gggcattgag gagcagcgtc agccgcagga
    13981 gagcgcgcag cagtatgttg tgcgtctggc acgcgagaaa gcacaggcgg gtgtcgcgca
    14041 aacggcacag gatctcccgg tgctgggtgc ggatattatc gttatcctga acggagaagt
    14101 gctcgagaaa ccgcgcgacg cagagcatgc ggcgcagatg ttgcgcaaat tatcgggtca
    14161 gacccatcag gtgatgacgg cagtggcgtt ggccgacagc cagcacattc tcgattgcct
    14221 ggtggtcacc gatgtgactt tcagaacgtt aacagacgaa gacatcgcgg gctatgtcgc
    14281 cagcggtgaa ccgttagata aagcaggtgc atacggtatt caggggctgg gtggctgttt
    14341 tgtcaggaag ataaatggca gctatcacgc cgtagtcggc ttaccgctgg ttgaaacgta
    14401 tgaattatta agtaatttta acgcactgcg tgagaaaagg gataaacatg acggctgaat
    14461 tgttagtaaa cgtaacgcct tcggaaacgc gagtggcgta tattgatggc ggtattctgc
    14521 aggaaattca tattgaacgt gaggcgcgac gcggaatagt aggcaatatc tacaagggtc
    14581 gtgtaagtcg tgtacttccg ggtatgcagg cggcttttgt agatattggg ctggataaag
    14641 ccgcgtttct tcatgcatcc gacatcatgc cgcataccga atgtgtggcg ggtgaagaac
    14701 aaaagcaatt cacggtgcgc gacatctcgg aactggttcg tcaggggcaa gatctgatgg
    14761 tgcaggtggt gaaagatccg cttggcacta aaggtgcgcg cctgaccacc gatatcacgc
    14821 tgccttctcg ctatctggta tttatgccag gggcttctca cgttggggtt tcccaacgaa
    14881 ttgaaagcga atcagaacgt gaacgtctga aaaaagtggt cgcagagtat tgcgacgagc
    14941 agggcgggtt tatcatccgc accgcagcgg aaggggttgg cgaggctgaa ctggcctccg
    15001 atgccgctta tctgaaacgc gtctggacca aagttatgga gcgtaaaaag cgcccgcaga
    15061 cccgttatca gctgtacggc gaactggcgc tggcgcagcg tgttctgcgt gattttgccg
    15121 acgccgaact ggaccgcatt cgcgttgact cacgcctgac ttacgaagca ttgctggagt
    15181 ttacctcgga gtacattccc gagatgacca gcaagctgga gcattacacc ggacgccagc
    15241 cgattttcga tctctttgat gtcgaaaacg aaatccagcg agcgttggaa cgcaaagtag
    15301 aactgaaatc cggtggttat ctgattatcg accagaccga agcgatgacc accgtggaca
    15361 tcaataccgg agcgtttgtc ggtcatcgca atctggacga cactattttc aataccaata
    15421 ttgaagcgac gcaggctatc gctcgccagt tacggttgcg taatctgggc gggattatca
    15481 ttattgattt catcgatatg aataatgaag atcaccgccg ccgcgtgctg cattcgctgg
    15541 agcaggcgtt gagcaaagac cgggtgaaaa ccagcgttaa tggtttttcg gcgctggggc
    15601 tggtggagat gacgcgtaaa cgcacccgcg aaagcattga gcacgtactg tgtaacgaat
    15661 gcccaacctg ccacggtcgc ggaacggtga aaaccgtgga aacggtatgc tatgaaatca
    15721 tgcgcgagat tgttcgtgtc caccatgctt acgactccga ccgtttcctg gtctatgctt
    15781 ctccggcagt agccgaagcc ttgaaaggcg aagagtcaca ctcgctggcg gaagtggaaa
    15841 ttttcgttgg caaacaggtt aaagtacaaa ttgaaccgct ctataaccag gagcagtttg
    15901 acgtcgtaat gatgtaaaca gatgctgggc cgccatccgg caaagggttt ttgagtcaca
    15961 tttttagcag acaaggagtg acgggtgagg cgattgccgg ggattttact gcttactgga
    16021 gccgcgctcg ttgtgatcgc tgccctgctg gttagcggcc tgcgtattgc tttaccgcat
    16081 cttaacgcct ggcgtccgga aatcctcaac aaaatagaat ccgcgactgg catgccggta
    16141 gaagccagtc agctctcagc cagctggcag aattttggcc cgacgcttga agcacacgac
    16201 atccgtgcag aactaaaaga tggcggcgaa ttttcggtta aacgcgttac tctggcgctg
    16261 gatgtctggc agagcctgtt acatatgcgc tggcagtttc gcgacctcac tttctggcag
    16321 ctgcgctttc gcaccaacac tcctatcacc agcggtggta gcgatgacag tctggaagcc
    16381 agtcacatca gcgatctgtt tcttcgtcaa tttgaccatt tcgatctccg cgacagtgaa
    16441 gtcagttttc tgacgccatc cggtcagcgc gccgagctgg cgatcccaca actcacctgg
    16501 ctgaacgatc cacgtcgaca ccgtgcggaa ggcctggtaa gcctctccag ccttaccgga
    16561 cagcacggcg tgatgcaggt gcgcatggat ttgcgcgatg atgaggggtt gttaagcaat
    16621 ggtcgcgtct ggctacaggc ggatgacatc gacctgaagc cgtggctcgg taaatggatg
    16681 caggacaata ttgctctgga aacggcacag ttctcccttg aaggctggat gacgatcgac
    16741 aaaggcgatg taaccggcgg tgacgtctgg ctgaaacagg gcggtgccag ctggttgggc
    16801 gagaagcaaa cgcatacgct gtcggtggat aatctgaccg cgcatattac gcgtgaaaat
    16861 ccgggctggc agttctctat tcccgataca cggatcacga tggacggcaa accctggccg
    16921 agcggagcat tgacgctggc ctggataccg gaacaggacg ttggcggcaa agacaataaa
    16981 cgcagtgacg aactccggat tcgcgctagt aatctggagc tggcaggcct ggagggcata
    17041 cgcccgctgg ccgcgaaact ttcacctgca ctgggtgatg tttggcgctc cacacaaccg
    17101 agcggcaaga ttaacactct ggcgctggat atcccgcttc aggcggcaga caagacccgt
    17161 tttcaggcat cgtggagcga tctggcctgg aagcaatgga aattattacc gggtgcggaa
    17221 cacttctccg ggacgctttc cggcagcgtt gaaaatggtt tgcttaccgc gtcgatgaag
    17281 caggcaaaga tgccttacga aacggtattc cgtgcgccac tggaaatcgc cgacggccag
    17341 gcaactataa gctggctgaa caatgacaaa ggtttccagc tggatgggcg taatattgac
    17401 gttaaagcca aagccgtcca tgcgcgcggc ggttttcgtt acctgcaacc tgctaacgat
    17461 gaaccctggc tgggtattct ggctggcatc agtaccgatg atggttcaca agcctggcgc
    17521 tatttcccgg aaaacttgat gggtaaagac ctggttgatt atttaagtgg tgcgattcag
    17581 ggcggtgaag cggataacgc gacgctggtt tatggtggca atccgcaact cttcccctat
    17641 aaacacaacg aaggtcagtt tgaagtgctg gtgccgctgc gcaacgcgaa gtttgccttc
    17701 cagccggact ggcctgcatt aactaacctt gatattgaac tggactttat taacgacggt
    17761 ttatggatga aaaccgatgg cgttaatctg ggcggcgtgc gcgcgagtaa tctcaccgca
    17821 gtgatccctg actactcaaa agaaaaactg ctgattgacg ctgacattaa aggtccgggt
    17881 aaagccgttg gcccttactt tgatgagaca ccgctgaaag attctctggg tgcgaccctg
    17941 caagaactcc agctcgacgg cgatgtgaat gctcgcttac atcttgatat cccgctgaac
    18001 ggcgaactgg taaccgcgaa aggtgaagtg acgctgcgta ataacagtct gtttatcaaa
    18061 ccactcgaca gcatcctgaa aaatttgagc ggtaaattca gctttatcaa tggcgatctg
    18121 caaagtgaac cactgacagc aagctggttt aatcagccgt tgaacgtgga tttttccacc
    18181 aaagaagggg caaaagccta ccaggttgcg gtgaatctca acggtaactg gcaaccggcg
    18241 aaaaccggcg ttctgcctga agcggtgaac gaagcattga gtggcagcgt ggcgtgggat
    18301 ggtaaagtgg gcattgatct gccttatcat gctggcgcga cctataacgt agagctgaac
    18361 ggcgatctga ataatgtgag tagtcactta ccttcaccgt tagccaaacc tgcgggtgaa
    18421 ccactagcgg taaacgttaa ggttgatggc aatctcaaca gctttgaatt aaccggacag
    18481 gctggtgcgg ataaccattt caatagccgc tggttgcttg gtcaaaagct gacgctcgat
    18541 cgtgctattt gggcggcaga cagtaaaacg ctcccgccgt tgccggaaca aagtggcgtt
    18601 gaacttaata tgccgccgat gaatggtgcc gagtggctgg ccctgttcca gaaaggcgct
    18661 gcggagagtg tcggtggtgc agcgagtttc ccacaacaca taacgttacg tacgcctatg
    18721 ttgtcactgg gaaatcagca atggaataac ctgagtattg tttcgcaacc gacggcaaat
    18781 ggcaccctgg ttgaggcgca agggcgtgaa atcaacgcca cgctagcgat gcgtaataac
    18841 gcgccgtggc tggcgaatat caaatatctt tattacaacc cgagcgtggc gaaaactcgt
    18901 ggtgattcaa cgccgtcatc acctttcccg acaacggagc gcattaactt ccgtggctgg
    18961 ccggacgcac aaatacgatg cacagagtgc tggttctggg ggcaaaaatt cggtcgcatt
    19021 gacagtgata tcaccatttc tggcgatacg ttaacgctga ccaatggact gattgatact
    19081 ggtttctcgc ggcttactgc cgatggtgaa tgggttaata atccggggaa tgaacgtacc
    19141 tcgctgaaag gaaaactgcg tgggcagaaa attgatgccg ccgcagaatt ttttggtgtc
    19201 acgacgccca tacgccagtc gtcatttaat gtggattacg atttacactg gcgcaaagca
    19261 ccctggcagc cagatgaagc gacgttgaat ggcatcattc atactcaact gggtaaaggc
    19321 gaaattaccg aaatcaatac cggacatgcc gggcaattgc tgcgcttatt gagcgtagat
    19381 gccctgatgc gtaagctgcg ttttgatttc agagacactt ttggcgaagg gttctatttt
    19441 gactccattc gcagcaccgc gtggattaaa gacggcgtta tgcacaccga cgacacgctg
    19501 gtggatggcc tggaggcgga tatcgccatg aaagggtcgg taaatctggt acgccgcgac
    19561 ctgaatatgg aagcggttgt cgcaccagag atttctgcga cggtgggcgt ggctgcggct
    19621 tttgcggtta accccattgt tggcgcggca gtgtttgccg ccagtaaagt gctggggccg
    19681 ctgtggagca aagtctccat tttgcgctat cacatttcgg gtccgctgga cgatccgcaa
    19741 atcaacgaag tgttgcgcca accgcgtaaa gaaaaagcgc aatgatttga cgagggcgcg
    19801 taattgcccc aatctcatat gataatcgtt gccaaaggcc aacgagccgg aacataactg
    19861 taggccggat agggcgttca cgccgcatcc ggcagccgta aaaaatcctc tactgcagta
    19921 actaacgagt agcaaaaacg atgagtctga acctggtaag tgaacaattg ctagcggcga
    19981 acggcctgaa acatcaggac ttgttcgcga tcctcggtca actggccgaa cgtcgccttg
    20041 attatggcga tctctatttt cagtcgagct atcacgaatc ctgggtttta gaagaccgca
    20101 ttattaaaga tggttcttac aacatcgatc agggcgttgg cgtgcgtgca atcagcggtg
    20161 aaaaaaccgg atttgcttac gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc
    20221 aagcggcgcg caccatcgtc cgtgatagtg gtgatggcaa agtacagacg ctgggcgcgg
    20281 tagagcatag cccgttgtat acctcggtag atccgctgca aagcatgagc cgtgaagaga
    20341 agctggatat cctgcgtcgc gtcgataagg ttgctcgcga agcggacaag cgcgtacagg
    20401 aagtgactgc cagcctcagt ggtgtctatg aattaattct ggttgcggcc accgacggca
    20461 cgctagcggc ggatgtccgt ccgctggtgc gtctttccgt gagcgttctg gtcgaagaag
    20521 atggcaaacg cgaacgcggt gccagtggcg gcggcggtcg ttttggttat gagttcttcc
    20581 ttgccgatct tgacggcgaa gtccgtgcgg atgcatgggc aaaagaagca gtgcgtatgg
    20641 cgctggtcaa tctttctgcc gttgctgcac cagcgggcac catgccggta gtacttggcg
    20701 caggttggcc gggcgtgctg ttgcatgaag cggtaggtca cggtctggaa ggcgacttca
    20761 accgccgtgg cacttcagta tttagtggac aggtcgggga gctggtggct tcagaactgt
    20821 gtaccgtggt tgatgacggc acgatggtcg atcgccgtgg ttcggtggcg attgatgatg
    20881 aaggtacgcc aggccagtac aacgtgctga ttgagaacgg cattctgaaa ggctacatgc
    20941 aggataaact caacgcgcgt ttgatgggga tgacgccgac tggcaacggt cgccgtgaat
    21001 cctacgccca tctgcccatg ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga
    21061 ccccgcaaga aattattgaa tccgttgagt acggtatcta tgcaccgaac tttggtggcg
    21121 gtcaggtgga tatcacctcc ggcaaattcg ttttctccac ttcagaagca tatctgattg
    21181 aaaacggtaa agtaacgaag ccggtgaaag gcgcaacgtt gattggttcc ggtatcgaaa
    21241 ccatgcagca gatttcgatg gttggcaacg acctgaaact ggataacggc gtgggtgtct
    21301 gcggtaaaga agggcaaagt ttgccggttg gcgtgggcca gccaacgttg aaagtcgata
    21361 acctgactgt tggcggtact gcgtaataat ttcattattt tcagaaggat aattaatctt
    21421 tctacgtgca cgaacggtcc cctcgctcct ctggggttag ggtgagggga acccgttggc
    21481 acaggtttgt agaacgtaac agtacaatat gaattacttc tctttcccgc gcccgtgcat
    21541 ctcctgaaac aatttaccga cctcaacaaa ataatccgtc agcgagttga tcacgacctg
    21601 taccttcagc ggcagcttat ctttttcggt atataacgca taaaccgggc gtggatctga
    21661 ctgataacgc ggcagcagga tctccagctc cccacgattg atctcgttga tcacccacat
    21721 cagcggcacg taggcgatcc cggcacccgc cgttagccag cgcaccagcg tcatcggatc
    21781 attagtcaca aatctccctt gcgggatcag gcgagtcgag atcccttccg gtgcaatcag
    21841 ttcaaattca ttgtcgggcc gcacgctgta ttcaagccat gaatggctac ttaaatcggc
    21901 gggtttttcc ggtatgccgt attgtgtgag atagcttttc gcggcgcaca ccaccattgg
    21961 catcgcgcca agacggcggg aaaacaggct ggaatcctgc aacgcgccga cgcggatcac
    22021 cacatccaga ccgtcggcaa tcaggtcggg ggctggaatt ccggtaacca gattggcgct
    22081 caaacctggg tattctttca gcattttggc tgtcagcccg gcgagaacat tttgtgccat
    22141 agttgaagaa cagccaatgc gtagcgtccc gatgggggtg ttattgaagg catacagttg
    22201 ctcatgaaca tcctgcactt catgaagcat acgacggcag ccctggtagt aaattctacc
    22261 ggcttcggtc aggccaatgc tgcgtgtgct acggtttaac agctttacct gcaactcatc
    22321 ttccagtttt gacaccgtct gactgatgga cgaaacgctc atctgtagct gtctggcggc
    22381 ggcggtaaaa gagccaaatt caactacttt ggcaaacacc gacatgcgtt ttagtcgttc
    22441 cattattcac tctgacttaa aagtgattta gatcacataa tatagataac agcataacag
    22501 ttacgctaat atattaaata tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc
    22561 ccttctctgc ggcgacagat gctgaaaata ataacgcctg ctctctctat acaaccaagg
    22621 tcaacatgag tctgtttccc gttatcgtgg tgtttgggct gtccttccca ccgatatttt
    22681 ttgaattgct tttatcactg gcgattttct ggctggtgcg ccgggtactt gtgccaacag
    22741 gtatctacga ctttgtctgg catccggcgt tgttcaacac cgcgctctat tgctgcttgt
    22801 tttatttgat atcgcgactg ttcgtttgag gttgaagtga aaacactaat aagaaaattc
    22861 tcccgtacgg ccatcacggt cgtattagtc attctggcct tcatcgcaat ttttaatgcc
    22921 tgggtctatt acaccgaatc cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt
    22981 gcgatcgcgc cggacgtttc tggactcatt acccaggtga atgttcatga taaccagctg
    23041 gtgaaaaaag gacagatact gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag
    23101 gaagcgcaag ccgatgttgc ttattatcag gtactggcac aggagaaacg ccaggaggcc
    23161 ggacgtcgta accgtctcgg tgtgcaggcg atgtctcgcg aagagatcga ccaggctaac
    23221 aacgtactac aaacggttct gcatcagtta gcgaaagcgc aggcgacccg cgatctggca
    23281 aaactggatc ttgaacgcac ggtgatccga gcgccagcag atggctgggt gaccaacctc
    23341 aacgtctata ccggtgagtt tattactcga ggatcaacgg cggttgcgct ggtgaaacag
    23401 aactccttct atgtactggc ctatatggaa gaaactaagc tggaaggggt gcgtccgggg
    23461 tatcgtgcag agatcacgcc gcttggcagt aacaaagtgc tgaaagggac tgttgatagt
    23521 gttgccgctg gggtcaccaa cgccagcagc acgcgtgacg acaaagggat ggcgaccatc
    23581 gactctgacc ttgaatgggt gcgtttggcg caacgtgttc cggttcgtat tcgtctcgac
    23641 aaccagcaag agaacatctg gcctgcgggc accactgcta cagtggtggt cactggcaaa
    23701 caagatcgcg acgaaagcca ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag
    23761 tttggttaat cacgatgggt attttctcca ttgctaacca acatattcgc tttgcggtaa
    23821 aactggcgac cgccattgta ctggcgctgt ttgttggctt tcacttccag ctggaaacgc
    23881 cacgctgggc ggtactgaca gcggcgattg ttgccgccgg tccggccttt gctgcgggag
    23941 gtgaaccgta ttctggcgct attcgctatc gtggcttttt gcgcatcatc ggcacattta
    24001 ttggctgtat tgccggactg gtgatcatca ttgcgatgat ccgcgcacca ttattgatga
    24061 ttctggtgtg ctgtatctgg gccggttttt gtacctggat atcctcgctg gtacgaatag
    24121 aaaactcgta cgcgtggggg ctggccggtt ataccgcgct gatcattgtg atcaccattc
    24181 agccggaacc attgcttacg ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg
    24241 gtattgtgtg tgcgattatg gcggatttgc tcttttctcc gcgatcgatc aaacaagaag
    24301 tagatcgaga gctggaaagt ttgctggtcg cgcaatatca attaatgcaa ctctgtatca
    24361 agcatggcga tggtgaagtt gtcgataaag cctggggcga cctggtgcga cgcaccacgg
    24421 cactacaagg catgcgcagc aacctgaata tggaatcttc ccgctgggcg cgggccaatc
    24481 gacgtttaaa agcgatcaat acgctatcgc tgacgctgat tacccaatcc tgcgaaactt
    24541 atcttattca gaatacgcgc ccggaattga tcactgatac tttccgcgaa ttttttgaca
    24601 cgccggtaga aaccgcgcag gacgtccaca agcagctcaa acgcctgcgg agagttatcg
    24661 cctggactgg ggaacgggaa acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc
    24721 gttatcagct tctcaagcgc ggcgttatca gtaacacaaa aatcaacgcc accgaagaag
    24781 agatcctgca aggcgaaccg gaagtaaaag tagagtcagc cgaacgtcat catgcaatgg
    24841 ttaacttctg gcgaaccaca ctttcctgca ttctgggcac gcttttctgg ctgtggacgg
    24901 gctggacttc cggcagtggt gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc
    24961 gtttgccgaa tccacgcatg gtggcgatcg actttatcta tgggacgctg gccgcgctgc
    25021 cgttagggct gctctacttt ttggtgatta tccctaatac ccaacagagc atgttgctgc
    25081 tgtgtattag cctggcagtg ctgggattct tcctcggtat agaagtacag aaacggcgac
    25141 tgggctcgat gggggcactg gccagcacca taaatattat cgtgctggat aacccgatga
    25201 ctttccattt cagtcagttt ctcgacagcg cattaggaca aatcgtcggc tgtgtgctcg
    25261 cgttcaccgt tattttgctg gtgcgggata aatcgcgcga caggactgga cgtgtactgc
    25321 ttaatcagtt tgtttctgcc gctgtttccg cgatgactac caatgtggca cgtcgtaaag
    25381 agaaccacct cccggcactt tatcagcagc tgtttttgct gatgaataag ttcccagggg
    25441 atttgccgaa atttcgcctg gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg
    25501 cgccgatccc ggttaacgag gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag
    25561 accatgtgat atctgcccgt agcgatgata aacgtcgtcg gtactttggc cagttgctgg
    25621 aagaactgga aatctaccag gaaaagctac gcatctggca agcgccaccg caggtgacgg
    25681 aaccggttca tcggctggcg gggatgctcc ataagtatca acatgcgttg accgatagtt
    25741 aagtcaaaac cgacgccaaa agcgtcggtt ttttcatggc tatacttagc gatgcacggc
    25801 agaactcgcc gcgaaacgtg acggtggcaa cagatgaata tttatgcctt tgattttgat
    25861 gagattgaga gtcaggagga tttttatcgt gactttagcc aaacctttgg tctggctaaa
    25921 gataaggtac gcgatctcga ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta
    25981 ccacttgaga ttgaatttgt tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta
    26041 atattgctgt ttgatgaggc agaggaagag ctggaagggc atttgcgttt taatgttcgt
    26101 cattagcgca aaaaaaagcc cacgaaccgg gggcaatatc gtcggacaaa acgatgaggg
    26161 tttatttgta cagttcagcc gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct
    26221 ggtaggccgt tgcgcctttc tcttccgctt ttttgttcag catttcacgt atatccattg
    26281 gcgaagacgc cacaccactt acggatacgg tcccgattgc ttcacgattt tgtgcttgtg
    26341 cagcatcaat ggagtcggca gcgaatgcac cgaaagagag aacagaaagt acgcttaatg
    26401 cagctacagt ggttttgatt ttcatgattt ttacctcgac ataatctttt agctgggcct
    26461 ttgtttcgtg acccatatca caaaatcaag tatacactaa ttattgaact aattaatacc
    26521 acactaactg tttcagttga tactaagtgt taaaaatgtg aattttaata acaaaaaagt
    26581 ataaaaaaca cggttattta ttattaatta tagtattttc aattagataa tgaattttgc
    26641 ataatgtgct ttttttaccg tatgtattca ttttgtgaat gatgtgtcgc ggtaaaacgc
    26701 actattcgtt aaatgtatcg caggagaaag aaggggaatg agagggataa gcaacatttt
    26761 ccccgccgcc agaagcgacg gggcagagat taaagctcct ggtcgaacag ctctaaaatc
    26821 gcttcgtaca ggtctttgac ggtgaaaccg ttagcagggg tggtaaagat ggtgtcatcg
    26881 ccagcgatgg tgcccagaat accttctgct ttgcccagtg agtccagcag gcgagcaatt
    26941 aactgcgccg cgccagggct ggtatgaatc acgacaactg catcgttgta gtcgatatcc
    27001 agcaccagat tcttcaatgg actggaggtg gttggtacac ccagttcagc tggcaggcag
    27061 taaaccattt ccattttggc attgcgtgta cgtacagcac caaacttggt caacatccgc
    27121 gagactttag actgattaat attgtcaaag ccttgctcct gcaacgcggc gacgatttcg
    27181 ccctgggagc taaatttctc ttctttaagt aatgctttaa atgctttaac tagttcttct
    27241 tgcttagccg agcttcgcat aagtcacccg atatggtggt tgatacaaca ttattgtgca
    27301 tacagatgaa tttttatgca aacagtcagc cctgaagaag gctgaaataa tgttatgaaa
    27361 gagcgggatt ttatcaaatt tcgttattga gaaacatgcc tgcgtcacgg catgcaaatt
    27421 ctgtttaaaa gtaaattaat tgttatcaaa ttgatgttgt tttggctgaa cggtagggta
    27481 tattgtcacc acctgttgga atgttgcgct aatgcataag cgactgttaa ttacgtaagt
    27541 taggttcctg attacggcaa ttaaatgcat aaacgctaaa cttgcgtgac tacacattct
    27601 tgagatgtgg tcattgtaaa cggcaatttt gtggattaag gtcgcggcag cggagcaaca
    27661 tatcttagtt tatcaatata ataaggagtt taggatgaaa gtcgcagtcc tcggcgctgc
    27721 tggcggtatt ggccaggcgc ttgcactact gttaaaaacc caactgcctt caggttcaga
    27781 actctctctg tatgatatcg ctccagtgac tcccggtgtg gccgtcgatc tgagccatat
    27841 ccctactgct gtgaaaatca aaggtttttc tggtgaagat gcgactccgg cgctggaagg
    27901 cgcagatgtc gttcttatct ctgcaggcgt agcgcgtaaa ccgggtatgg atcgttccga
    27961 cctgtttaac gttaacgccg gcatcgtgaa aaacctggta cagcaagttg cgaaaaactg
    28021 cccgaaagcg tgcattggta ttatcactaa cccggttaac accacagttg caattgctgc
    28081 tgaagtgctg aaaaaagccg gtgtttatga caaaaacaaa ctgttcggcg ttaccacgct
    28141 ggatatcatt cgttccaaca cctttgttgc ggaactgaaa ggcaaacagc caggcgaagt
    28201 tgaagtgccg gttattggcg gtcactctgg tgttaccatt ctgccgctgc tgtcacaggt
    28261 tcctggcgtt agttttaccg agcaggaagt ggctgatctg accaaacgta tccagaacgc
    28321 gggtactgag gtggttgaag cgaaagccgg tggcgggtct gcaaccctgt ctatgggcca
    28381 ggcagctgca cgttttggtc tgtctctggt tcgtgcactg cagggcgaac aaggcgttgt
    28441 cgaatgtgcc tacgttgaag gcgacggtca gtacgcccgt ttcttctctc aaccgctgct
    28501 gctgggtaaa aacggcgtgg aagagcgtaa atctatcggt accctgagcg catttgaaca
    28561 gaacgcgctg gaaggtatgc tggatacgct caagaaagat atcgccctgg gcaaagagtt
    28621 cgttaataag taattgatta gcgaataata aaaaaccgga gcacagactc cggtttttgt
    28681 tttgagcact cgacttaatt ggttgccgga tattcctgaa tggtgacctg cagcgttaac
    28741 tgcttatcat cacgcatcac tacaaccggg atcaccgaac cagggcgaat ttccgccacc
    28801 tgatccatcg tctccagagc agagatggcc ggtttgttat ccaccgaaat aatcagatcg
    28861 ttgacctgaa tacccgcatt cgccgctggg ccgtcaggtg acacttcatt aaccacgatc
    28921 ccttgcagtt gatctatacc accgctctgc gcgtgcagtg gtgcgatctc gcgtccgccg
    28981 ataccaatgt agccgcggat cacgcgacca tcgcggatca gcttatccat aattttggtt
    29041 gctaactgga aaggaatcgc aaagccgata ccttccggcg tttcgccatc gttactctta
    29101 tcaaacgaca gcgtgttaat gcccatcagt tctcccagcg agttcaccag cgcgccgcca
    29161 gagttaccgt ggttaatgga agcatcggtt tgtaggaagt tttgccgccc ggtcgggttc
    29221 agaccgattc gacccgtggc actaataatc ccttgggtaa tggtctgccc gaggttgtac
    29281 gggttaccga tcgccagtac tacgtcgcca atgtgcggta cgcgacgtgc attaattgga
    29341 atggtaggta aaccgccagt ggcattaatt ttaagtaccg ccagatcggt tagagagtca
    29401 gatcccacca gcaatgcttc aaatacacgt ccatcctgta aggcgacgat gatctgatcg
    29461 gcgtcgttga tgacgtgttt attggtgatg atataaccgc gttgatccat gattacaccg
    29521 gatcccaggg tgcggatctc aagctggttg tgagagttgg tgttcaaacc acggttgtaa
    29581 acgttaacca ccgctggcgc ggcgcggcga accgccagat tatagctggc aggcgtctca
    29641 tcggtactgt caaattgcgg agtggaaagc gggttaaggc tgcgcagcga aggcatggca
    29701 accagcagaa tagcgccgac aattaatcca atcgcaacgg aacgtaagag cttcacaaac
    29761 atgatggagg cgtcattaaa aaagggaacg gcagcagcat accacgagtt aaccggacat
    29821 cacacgtcag cctgatgccc ggtttacgac attaacgcat cagcagatag atgctttcat
    29881 tgccgcgtac aatttgcagg gcgatgatgg ccggttttgc cgccagcact ttacgcattt
    29941 cagcaatcga gttcacccga tcgcggttga cgccaatgat cacatcgtct ttttgcaagc
    30001 cagcctgagc agctgggctt cctttgacaa cttcatctat cttaatacct ttgccgccat
    30061 cttttagctg accatcgctc aacgttgcac cttccagcgc tggccgacga agaggtgctg
    30121 gtatcaagcg tcacttctac ttccagtggt ttgccgttac gcagcaggcc aagcttcact
    30181 ttcgtgcccg gctctgtagt cgcgatacga gagcgcaact cagcaaagct attcagcggt
    30241 ttgccgttga ggctggtaat aatatcgccc gctttgacgc ccgctttcgc tgagccagaa
    30301 cttggcaaca cttcgctgac aaacgcgcca cgctgcacgt caaggttgaa ggctttggcg
    30361 atatcggcac tcatctcggt gcctttgatg cctaacaaac cgcgtttgat ttcaccaaag
    30421 tcgataagct gctgcgccag tgttcgcgcc atattactgg ggatggcaaa tccaatcccg
    30481 acgctcccgc cgccaggcgc aaggattgca gtgttgatgc caattaactc accgttaagg
    30541 tttaatagtg caccgccgga gttaccgcgg ttaatggaag catctgtctg gataaagttt
    30601 tccagacctt caagattcaa cccgctgcgg cctaatgcgg aaacaatgcc agaggtggcg
    30661 gtttgcccaa ggccaaatgg gttaccgacc gctacggcaa aatcaccgac gcgcaattta
    30721 tcggagtcgg caatagcgat ttgcgttaat ttgctcgggt tttgaatttg taacagggcg
    30781 atatcgctct ggtcatcgct accaatcagt tttgcatcaa actcgcgccc atcattgagc
    30841 tgaatactga ttttctgtgc ctgattaatc acatggttgt tggtcagcac atagccttta
    30901 ctggcgttga tgatgacacc ggaacctaaa ccttcgaagg gttgtgcagg ttgatccggt
    30961 aaatcatcac caaaaaactt tttgaattct tccgggattt tctgtccctg actggccgtt
    31021 ccttccaccc gtacgctcac cactgccgga agcacttttt ccagcattgg agccagactg
    31081 gggagagggg cctgatcggc aacctggcct ggaatcgacg cgacggcctg aaatgacgcc
    31141 gagagagtta acccggcact taacgctaat gcactcaaca gctgggtttg ttttttcatt
    31201 attcctgctc tcgtacctga atgataagaa aagagattca aaacagtttg atgttattga
    31261 attttcagac ggctaacaat gagacaccag attaaatgat agtcggggag ggagagaaga
    31321 ggagggcgca atggctgcgc ccgaaaaata aattagtcgc gcttcgcgcc agtacgcagc
    31381 aggccggatg cgccttcaga atagtcgcga ggcacctgca ccggtgcctg atcgttgctg
    31441 gcttcagact ctgccagacg attacggaac gggtttgctt cagcagacag ttccggcagc
    31501 aggctgctgg agctttttgc catgtgctga tacagctggc gatagtcgtg cgccatggta
    31561 tccagtaatt ccgcgctgcg ggcaaagtgg ctaaccagct cttcgcgata ctcgtccagt
    31621 tcagctttat tcttttccag ttcgtactgc aacgcctgtt gctggcgtag tttacgatta
    31681 ccaaaacgca tggccacagc accaataatg atgccgacga ctaacccaat tagcgcatat
    31741 tcccaggtca tgaacatctc ccgttgtctt ttgtttccgt agggtgttgg cttcaggctc
    31801 catgcctgcg gctgattatg ccactataac cgttaattcc acagaagtgg aatcccgact
    31861 gcatatcgcg tagtgtagaa cggccttttt ttcgtcaacc gtgaacaacg gcgcaccgat
    31921 tattcaagga ataacaataa gatcatgcaa agcgttaccc caacatcgca atacctgaag
    31981 gcgctcaatg aaggcagcca tcaacccgac gacgttcaaa aagaggccgt cagccgcctg
    32041 gaaattattt atcaagaact catcaatagc aggccaccag cccccaggac gagtgggcta
    32101 atggcgcggg tcggtaagct gtggggtaaa cgcgaagaca caaagcatac gccagtgcgt
    32161 ggcttatata tgtggggcgg tgtaggacgc gggaaaacct ggctgatgga ccttttctat
    32221 caaagcctgc cgggagagcg gaaacagcgc ctgcactttc accgttttat gctgcgggtg
    32281 cacgaagagc taactgcctt acaggggcag accgatccgc tggaaattat tgccgatcgc
    32341 tttaaagccg aaactgacgt gctctgtttt gacgaatttt ttgtttctga tattaccgac
    32401 gccatgctac ttggcggtct gatgaaagcc ctgttcaccc gcggtattac cctggtagcg
    32461 acgtcaaata ttccgccgga cgaactttat cgaaatggcc tgcaacgtgc gcgttttctg
    32521 cctgcaatcg atgccattaa acagcattgt gatgtaatga acgtggacgc tggtgttgat
    32581 tatcgactgc gtacactcac tcaggcgcat ctgtggcttt cgccactcaa cgatgaaacc
    32641 cgggcgcaga tggataaact atggttggcg ctggcggggg tgaaacgaga aaattcaccg
    32701 acgttagaaa tcaaccatcg gccattggcg acaatgggcg tcgagaacca gacgctggcg
    32761 gtttctttta ctacgctgtg cgtcgacgcc cgcagtcagc atgactatat tgcgctctca
    32821 cgcctgtttc acacagtaat gttgtttgat gtgccagtca tgacgcggtt gatggagagc
    32881 gaagcgcggc gctttattgc gctggtggat gagttttacg agcgccatgt caaattagtg
    32941 gtgagtgcag aagtgccgct gtatgaaatt tatcagggtg agcggctgaa gtttgagttc
    33001 cagcgttgcc tgtcacgtct gcaagagatg caaagcgaag agtatctgaa gcgcgagcat
    33061 ttggcgggtt aaaacctgcc acaaatcaca aaaaggggtc gatctttgac cccgacttct
    33121 ctataatcct gcgaccccac gttacaagaa agtttttttc ccaaaacttt ttgtgtgctg
    33181 gcataggcta ttcgaagggg taggtttgcc ggactttgtc gtgtgaacct caacaattga
    33241 agacgtttgg gtgttcacca acgtgtaact atttattggg taagctttta atgaaaactt
    33301 ttacagctaa accagaaacc gtaaaacgcg actggtatgt tgttgacgcg accggtaaaa
    33361 ctctgggccg tctggctact gaactggctc gtcgcctgcg cggtaagcac aaagcggaat
    33421 atactccgca cgtagatacc ggtgattaca tcatcgttct gaacgctgac aaagttgctg
    33481 taaccggcaa caagcgtact gacaaagtgt actatcacca caccggccac atcggtggta
    33541 tcaaacaagc gacctttgaa gagatgattg ctcgccgtcc tgagcgtgtg attgaaatcg
    33601 cggttaaagg catgttgcca aaaggcccgc tgggtcgtgc tatgttccgt aaactgaaag
    33661 tttacgcggg taacgagcac aaccacgcgg cacagcaacc gcaagttctt gacatctaat
    33721 cgggattata ggcaatggct gaaaatcaat actacggcac tggtcgccgc aaaagttccg
    33781 cagctcgcgt tttcatcaaa ccgggcaacg gtaaaatcgt aatcaaccaa cgttctctgg
    33841 aacagtactt cggtcgtgaa actgcccgca tggtagttcg tcagccgctg gaactggtcg
    33901 acatggttga gaaactggac ctgtacatca ccgttaaagg tggtggtatc tctggtcagg
    33961 ctggtgcgat ccgtcacggt atcacccgcg ctctgatgga atacgacgag tccctgcgtt
    34021 ctgaactgcg taaagctggc ttcgttactc gtgacgctcg tcaggttgaa cgtaagaaag
    34081 tcggtctgcg taaagcacgt cgtcgtccgc agttctccaa acgttaattg gcttctgcta
    34141 cggcagaaaa caatttgcga aaaaacccgc ttcggcgggt ttttttatgg ctaaattctg
    34201 aatcagcgta aaaaatggga agttgctttt tgctgccgtc tgaaagacag gtaaaacaaa
    34261 aaccacatcg ccaataaggg tctaagtcaa ctatttcaga ctaaagcgca tctctttttc
    34321 cccatttccg gcatccactc accacaaagg tcgcaaaatc tggtaaacta tcatccaatt
    34381 ttctgcccaa atgtcgggta ttgctcattt tttgtttgat tttcgaacaa agagagtcgt
    34441 tccttattgg gtaacacaac ttctgactgg ccacctggtg gctggtagca gtaaaaattc
    34501 tgactatacc tggaggtttt catggctgtc gctgccaaca aacgttcggt aatgacgctg
    34561 ttttccggtc ctactgacat ctatagccat caggtccgca ttgtgctggc tgagaaaggt
    34621 gtaagtttcg agatcgaaca cgtggaaaag gacaatccgc ctcaggatct gattgacctc
    34681 aacccgaatc agagcgttcc gaccctggtg gatcgtgagc tgaccctgtg ggaatctcgc
    34741 atcattatgg aatatctgga tgagcgtttc ccgcatccgc cactgatgcc tgtttacccg
    34801 gtagctcgcg gtgaaagccg tctgtacatg catcgcatcg aaaaagactg gtacacgctg
    34861 atgaacacta tcatcaacgg ttcagcttct gaagcagatg ccgcacgtaa gcaactgcgc
    34921 gaagaactgc tggcgattgc gccggtcttc ggtcagaagc cgtacttcct gagcgatgag
    34981 ttcagcctgg tcgattgcta ccttgctccg ctgctgtggc gtctgccgca actgggcatc
    35041 gagttcagcg gcccgggtgc gaaagagctg aaaggctata tgacccgcgt ctttgagcgt
    35101 gactctttcc ttgcttcttt aactgaagca gaacgtgaaa tgcgtctggg ccggagttaa
    35161 tctgtatgga tttgtcacag ctaacaccac gtcgtcccta tctgctgcgt gcattctatg
    35221 agtggttgct ggataaccag ctcacgccgc acctggtggt ggatgtgacg ctccctggcg
    35281 tgcaggttcc tatggaatat gcgcgtgacg ggcaaatcgt actcaacatt gcgccgcgtg
    35341 ctgtcggcaa tctggaactg gcgaatgatg aggtgcgctt taacgcgcgc tttggtggca
    35401 ttccgcgtca ggtttctgtg ccgctggctg ccgtgctggc tatctacgcc cgtgaaaatg
    35461 gcgcaggcac gatgtttgag cctgaagctg cctacgatga agataccagc atcatgaatg
    35521 atgaagaggc atcggcagac aacgaaaccg ttatgtcggt tattgatggc gacaagccag
    35581 atcacgatga tgacactcat cctgacgatg aacctccgca gccaccacgc ggtggtcgac
    35641 cggcattacg cgttgtgaag taatacaaaa caggcccagg cggcctgttt tgtcttttta
    35701 atgacttaat aaaatagcgt gattgtggca atcatattca ccacgaaacc gactgccatg
    35761 gggatcgctg tccgcttcac caccgcaaaa ggcgaaacgc ctgcaattcc tgacgtaacg
    35821 accaccaccg cagtaatcgg cgaaaccgcg cgcgctagcg tcgtggcaaa atgcatcggc
    35881 ataatcatta caaccgctgg tacatgtagt ccggctgcga tattcggaat aagactggca
    35941 aatgacataa acggcgcatt gccagagccc atcacaatgg cacaaatggc aatgactagc
    36001 gccataataa tcatcacgcc aataccgccc agaccagaat gctccgcacc cctgataacc
    36061 gcatcaacag tgccaatcgt cgttaagcct ttcgcaaata tttcacccgc gaccaccagc
    36121 gttaccacgt tgacaaactg cgtacccatg ccgtcaaaaa acgcctgcac atcgtccatt
    36181 gtttcgcgca agttatgctt gcggaagaac tcaacaaaca tcgtcaccgt caaacttagt
    36241 aacatcacca ccaccagatg cagttctgac tgcatcagcc cgacgtgggc gaggaacagc
    36301 gagccgagca tcaggattaa cggcatcaca ggtaaaatgg cgtaatagag cggcgggaca
    36361 ttatcgagag ttttttgctc tgcctgttcg tgattgatat ttttatcttt tttgtcaaaa
    36421 gcgcgttgca cgaaaaagtg ggagatcgcc accgagataa tgacgcaaga ggcgaccgga
    36481 agctggtagt ggaagaagta tgtggcaatt ttcattcccg cgacctgggc tgcaaaaatg
    36541 gagttcgttt ccagaatccc ccactcaatg gacatcgtgg ttgcgataac cgccaccgca
    36601 gagaggcgac ttactcccag actcaccagc gtcggaaata aggtgaccat cagcaacata
    36661 cccagaccgg aggcgctggt aataaactgc gccatgattt ggccgatgac gtaagttgcc
    36721 gacagaataa tatacggcga gcgaatgagt tttaacgggc ggcttagcaa gctcaccatc
    36781 gcgcaactgg ctcctatgcg ctccatgtag cgggcataac cgcccaccgc cataatcgac
    36841 agccccaatc cggcaatgcg gttggacagc atgcgcagga tttcattata aatgtcgacg
    36901 atcaagtagc cgctgctttt ggcgggatcg agtacaccac cgaaccctaa ccagactccg
    36961 cacatcatca ggaagacacc tgcggcagcc agcaccacct gaggtttgta gtttttcagg
    37021 atcaaatagc ccatcgtaat taatacgatg acagatataa ttatgccgaa catt
//
LOCUS       DAJVQR010000031        36587 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVQR010000031 DAJVQR010000000
VERSION     DAJVQR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(156..1391)
                     /locus_tag="OQM52_001668"
     CDS             complement(156..1391)
                     /locus_tag="OQM52_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1563559.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OQM52_001669"
     tRNA            complement(1538..1614)
                     /locus_tag="OQM52_001669"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OQM52_001670"
     tRNA            complement(1657..1743)
                     /locus_tag="OQM52_001670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OQM52_001671"
     tRNA            complement(1764..1839)
                     /locus_tag="OQM52_001671"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OQM52_001672"
     tRNA            complement(1898..1974)
                     /locus_tag="OQM52_001672"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OQM52_001673"
     CDS             complement(2077..3462)
                     /locus_tag="OQM52_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1563560.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OQM52_001674"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OQM52_001674"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1563561.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFECIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OQM52_001675"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OQM52_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1563562.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OQM52_001676"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OQM52_001676"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1563563.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OQM52_001677"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OQM52_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1563564.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OQM52_001678"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OQM52_001678"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1563565.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OQM52_001679"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OQM52_001679"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1563566.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OQM52_001680"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OQM52_001680"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1563567.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OQM52_001681"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OQM52_001681"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1563568.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OQM52_001682"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OQM52_001682"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1563569.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OQM52_001683"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OQM52_001683"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1563570.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OQM52_001684"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OQM52_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1563571.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OQM52_001685"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OQM52_001685"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1563572.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OQM52_001686"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OQM52_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1563573.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OQM52_001687"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OQM52_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1563574.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OQM52_001688"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OQM52_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1563575.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OQM52_001689"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OQM52_001689"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1563576.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OQM52_001690"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OQM52_001690"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1563577.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OQM52_001691"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OQM52_001691"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1563578.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OQM52_001692"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OQM52_001692"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1563579.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OQM52_001693"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OQM52_001693"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1563580.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OQM52_001694"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OQM52_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1563581.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OQM52_001695"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OQM52_001695"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1563582.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OQM52_001696"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OQM52_001696"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1563583.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OQM52_001697"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OQM52_001697"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1563584.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OQM52_001698"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OQM52_001698"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1563585.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OQM52_001699"
     CDS             complement(31267..32913)
                     /locus_tag="OQM52_001699"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1563586.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OQM52_001700"
     CDS             complement(32916..32966)
                     /locus_tag="OQM52_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563587.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OQM52_001701"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OQM52_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1563588.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="OQM52_001702"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="OQM52_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OQM52_001703"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OQM52_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1563589.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="OQM52_001704"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="OQM52_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1563590.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36398..36473)
                     /locus_tag="OQM52_001705"
     tRNA            complement(36398..36473)
                     /locus_tag="OQM52_001705"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="OQM52_001706"
     tRNA            complement(36482..36558)
                     /locus_tag="OQM52_001706"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8940 a        10012 c         9216 g         8419 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc attcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gatgtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAJVQR010000032        35748 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVQR010000032 DAJVQR010000000
VERSION     DAJVQR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..247
                     /locus_tag="OQM52_001707"
     CDS             <1..247
                     /locus_tag="OQM52_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1563591.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OQM52_001708"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OQM52_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1563592.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OQM52_001709"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OQM52_001709"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1563593.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="OQM52_001710"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="OQM52_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCS1563594.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKLTALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="OQM52_001711"
     CDS             complement(3258..3542)
                     /locus_tag="OQM52_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563595.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OQM52_001712"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OQM52_001712"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1563596.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="OQM52_001713"
     CDS             complement(4443..5651)
                     /locus_tag="OQM52_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1563597.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="OQM52_001714"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="OQM52_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1563598.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="OQM52_001715"
     CDS             complement(6446..8131)
                     /locus_tag="OQM52_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1563599.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="OQM52_001716"
     CDS             8401..8778
                     /locus_tag="OQM52_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1563600.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OQM52_001717"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OQM52_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1563601.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="OQM52_001718"
     CDS             9225..9512
                     /locus_tag="OQM52_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1563602.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="OQM52_001719"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="OQM52_001719"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1563603.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="OQM52_001720"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="OQM52_001720"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1563604.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="OQM52_001721"
     CDS             11269..11745
                     /locus_tag="OQM52_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1563605.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="OQM52_001722"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="OQM52_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1563606.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="OQM52_001723"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="OQM52_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1563607.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="OQM52_001724"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="OQM52_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1563608.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="OQM52_001725"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="OQM52_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1563609.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="OQM52_001726"
     CDS             16302..16790
                     /locus_tag="OQM52_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1563610.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="OQM52_001727"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="OQM52_001727"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1563611.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OQM52_001728"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OQM52_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1563612.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OQM52_001729"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OQM52_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1563613.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OQM52_001730"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OQM52_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1563614.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OQM52_001731"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OQM52_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1563615.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OQM52_001732"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OQM52_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1563616.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="OQM52_001733"
     CDS             complement(22242..22757)
                     /locus_tag="OQM52_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1563617.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="OQM52_001734"
     CDS             23406..25175
                     /locus_tag="OQM52_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1563618.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="OQM52_001735"
     CDS             25386..25709
                     /locus_tag="OQM52_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1563619.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="OQM52_001736"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="OQM52_001736"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1563620.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="OQM52_001737"
     CDS             complement(26533..27546)
                     /locus_tag="OQM52_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1563621.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="OQM52_001738"
     CDS             complement(27645..29075)
                     /locus_tag="OQM52_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1563622.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OQM52_001739"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OQM52_001739"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1563623.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OQM52_001740"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OQM52_001740"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1563624.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OQM52_001741"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OQM52_001741"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1563625.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OQM52_001742"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OQM52_001742"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1563626.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OQM52_001743"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OQM52_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1563627.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8908 a         8838 c         9185 g         8817 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagctcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttctcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatgctaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaactgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg cgccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta tttactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgccagcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcttggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggg cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctagtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caagtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aatgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 tttccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgcgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttaa
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggcgac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat cccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga ggcgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt ccagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 gcgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatg
//
LOCUS       DAJVQR010000033        34967 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVQR010000033 DAJVQR010000000
VERSION     DAJVQR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..783)
                     /locus_tag="OQM52_001744"
     CDS             complement(<1..783)
                     /locus_tag="OQM52_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1563628.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="OQM52_001745"
                     /pseudo
     CDS             875..961
                     /locus_tag="OQM52_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1366..1842)
                     /locus_tag="OQM52_001746"
     CDS             complement(1366..1842)
                     /locus_tag="OQM52_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1563629.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OQM52_001747"
     CDS             complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OQM52_001747"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1563630.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3277..4317
                     /gene="bioB"
                     /locus_tag="OQM52_001748"
     CDS             3277..4317
                     /gene="bioB"
                     /locus_tag="OQM52_001748"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1563631.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4314..5468
                     /gene="bioF"
                     /locus_tag="OQM52_001749"
     CDS             4314..5468
                     /gene="bioF"
                     /locus_tag="OQM52_001749"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1563632.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5455..6210
                     /gene="bioC"
                     /locus_tag="OQM52_001750"
     CDS             5455..6210
                     /gene="bioC"
                     /locus_tag="OQM52_001750"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1563633.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6203..6880
                     /gene="bioD"
                     /locus_tag="OQM52_001751"
     CDS             6203..6880
                     /gene="bioD"
                     /locus_tag="OQM52_001751"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1563634.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7459..9480
                     /gene="uvrB"
                     /locus_tag="OQM52_001752"
     CDS             7459..9480
                     /gene="uvrB"
                     /locus_tag="OQM52_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1563635.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OQM52_001753"
     CDS             complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OQM52_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1563636.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10845..10989
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10977..11966
                     /gene="moaA"
                     /locus_tag="OQM52_001754"
     CDS             10977..11966
                     /gene="moaA"
                     /locus_tag="OQM52_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1563637.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11988..12500
                     /gene="moaB"
                     /locus_tag="OQM52_001755"
     CDS             11988..12500
                     /gene="moaB"
                     /locus_tag="OQM52_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1563638.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12503..12988
                     /gene="moaC"
                     /locus_tag="OQM52_001756"
     CDS             12503..12988
                     /gene="moaC"
                     /locus_tag="OQM52_001756"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1563639.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12981..13226
                     /gene="moaD"
                     /locus_tag="OQM52_001757"
     CDS             12981..13226
                     /gene="moaD"
                     /locus_tag="OQM52_001757"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1563640.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13228..13680
                     /gene="moaE"
                     /locus_tag="OQM52_001758"
     CDS             13228..13680
                     /gene="moaE"
                     /locus_tag="OQM52_001758"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1563641.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13817..14521
                     /locus_tag="OQM52_001759"
     CDS             13817..14521
                     /locus_tag="OQM52_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1563642.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14726..15439
                     /locus_tag="OQM52_001760"
     CDS             14726..15439
                     /locus_tag="OQM52_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1563643.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15475..16431)
                     /locus_tag="OQM52_001761"
     CDS             complement(15475..16431)
                     /locus_tag="OQM52_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCS1563644.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OQM52_001762"
     CDS             complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OQM52_001762"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1563645.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17669..18430)
                     /locus_tag="OQM52_001763"
     CDS             complement(17669..18430)
                     /locus_tag="OQM52_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1563646.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18563..18973
                     /locus_tag="OQM52_001764"
     CDS             18563..18973
                     /locus_tag="OQM52_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1563647.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18935..20041)
                     /locus_tag="OQM52_001765"
     CDS             complement(18935..20041)
                     /locus_tag="OQM52_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1563648.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20052..21185)
                     /locus_tag="OQM52_001766"
     CDS             complement(20052..21185)
                     /locus_tag="OQM52_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1563649.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21178..22914)
                     /locus_tag="OQM52_001767"
     CDS             complement(21178..22914)
                     /locus_tag="OQM52_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1563650.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OQM52_001768"
     CDS             complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OQM52_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1563651.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OQM52_001769"
     CDS             complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OQM52_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1563652.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24805..25992
                     /gene="rhlE"
                     /locus_tag="OQM52_001770"
                     /pseudo
     CDS             24805..25992
                     /gene="rhlE"
                     /locus_tag="OQM52_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OQM52_001771"
     CDS             complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OQM52_001771"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1563653.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26801..28951
                     /gene="dinG"
                     /locus_tag="OQM52_001772"
     CDS             26801..28951
                     /gene="dinG"
                     /locus_tag="OQM52_001772"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1563654.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28979..29941
                     /gene="ybiB"
                     /locus_tag="OQM52_001773"
     CDS             28979..29941
                     /gene="ybiB"
                     /locus_tag="OQM52_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1563655.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30082..31167
                     /gene="hcxB"
                     /locus_tag="OQM52_001774"
     CDS             30082..31167
                     /gene="hcxB"
                     /locus_tag="OQM52_001774"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1563656.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31396..31656)
                     /locus_tag="OQM52_001775"
     CDS             complement(31396..31656)
                     /locus_tag="OQM52_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1563657.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="OQM52_001776"
     CDS             complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="OQM52_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1563658.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="OQM52_001777"
     CDS             complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="OQM52_001777"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1563659.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32980..34967)
                     /locus_tag="OQM52_001778"
                     /pseudo
     CDS             complement(32980..34967)
                     /locus_tag="OQM52_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8285 a         8969 c         9447 g         8266 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 tctaagtggt tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat
     1201 tttttaagct aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca
     1261 gcttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat
     1321 acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc
     1381 ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct
     1441 ggctccttca tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg
     1501 atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg
     1561 cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac
     1621 gcgggtatca gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc
     1681 atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc
     1741 cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg
     1801 caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt
     1861 tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt
     1921 catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa
     1981 tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga
     2041 caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg
     2101 cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc
     2161 gcagctgcac ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta
     2221 ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa
     2281 agcagccggc ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg
     2341 aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt
     2401 ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg
     2461 caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat
     2521 ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt
     2581 catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac
     2641 catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca
     2701 ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat
     2761 aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca
     2821 acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact
     2881 ccagcggttg cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg
     2941 tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa
     3001 gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc
     3061 gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca
     3121 gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt
     3181 ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc
     3241 gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca
     3301 ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag
     3361 caggtgcatc gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt
     3421 aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc
     3481 gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg
     3541 aaagcggcag gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc
     3601 gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt
     3661 atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat
     3721 tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc
     3781 acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc
     3841 tcaggtggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa
     3901 ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa
     3961 ggcacgccgc ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc
     4021 gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg
     4081 aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc
     4141 aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg
     4201 gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt
     4261 ggacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct
     4321 ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc
     4381 gttatccggt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga
     4441 acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga
     4501 agcagggggc ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata
     4561 gcgtggtgca tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc
     4621 tgctgtttat ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag
     4681 aggaccgtat tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa
     4741 gcccgtcgca gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg
     4801 cttccccctg tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg
     4861 atagcgcgcc actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg
     4921 tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc
     4981 aaaaggtaaa accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg
     5041 ctgcggtgct ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta
     5101 tctacagcac cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca
     5161 ttcgccgtga tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc
     5221 gtgccggagt acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat
     5281 tgatcgtcgg tgataacagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct
     5341 gctgggtcac ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa
     5401 cgctaaccgc tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca
     5461 acggttaata aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa
     5521 catgcagatc tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa
     5581 tacacccacg tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa
     5641 cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat
     5701 aaagatgccg cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg
     5761 acgttcgatc ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca
     5821 ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg
     5881 cagggatcgt tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct
     5941 aatcgctttt taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat
     6001 catattcagc tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa
     6061 ggcatcggtg ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag
     6121 ttgcagcgat tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat
     6181 ctttttttgg gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga
     6241 agtggggaaa accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg
     6301 gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa
     6361 tagtgacgcg ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa
     6421 tccttacacc ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag agggcaggcc
     6481 gatagaatca ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt
     6541 gttagtggaa ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga
     6601 ttgggtaaca caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat
     6661 taatcacgcg atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg
     6721 ggtggcgaac gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac
     6781 ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa
     6841 tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa
     6901 ttgcgagcga gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat
     6961 cacgaaaaaa attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg
     7021 ggataggggc tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta
     7081 gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc
     7141 tcaaagaaat atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca
     7201 ggcatctctt gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc
     7261 tttggggata acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg
     7321 attgacagcg gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc
     7381 agtataattt gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac
     7441 tccttcaggt agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct
     7501 ggcgatcagc cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac
     7561 cagacgttac ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct
     7621 gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat
     7681 ggcgaaatga aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac
     7741 tactatcagc cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg
     7801 gttaacgaac atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt
     7861 gatgtgattg tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat
     7921 ctcaagatga tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc
     7981 cgactggcgg agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc
     8041 gttcgtggcg aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg
     8101 gaactgtttg acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt
     8161 gtttccacta ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag
     8221 cgcatcgtac aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg
     8281 ttggaaaaca acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg
     8341 gagatgatgg acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc
     8401 ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg
     8461 ctggtcgtcg atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac
     8521 cgggcgcgta aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac
     8581 cgtccgctta agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg
     8641 acgccgggta attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt
     8701 ccaaccggat tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat
     8761 cttctttcgg agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg
     8821 accaagcgaa tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt
     8881 tatcttcact cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg
     8941 ggtgagttcg acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa
     9001 gtgtcgctgg tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg
     9061 ttgatccaga ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc
     9121 gataggagca ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa
     9181 cagcagaagt acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc
     9241 gatatcctgg cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg
     9301 cgcccgattg ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag
     9361 aaaatccatg aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa
     9421 gcggcacaaa ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa
     9481 caggatagcg aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag
     9541 gcctacattt tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc
     9601 acgccgcatc cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct
     9661 tcggatttcc gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg
     9721 cggtcatgac gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct
     9781 ttcaccgccg agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca
     9841 acatactgct ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc
     9901 aactcacgcc ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg
     9961 gcgatttcct tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata
    10021 atgatgagat ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc
    10081 gttaacaata actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact
    10141 tcatgcccct gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa
    10201 tgcgtatcca ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta
    10261 aggtgatcca gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg
    10321 ccgccaaaac ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg
    10381 aggcagttgc gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc
    10441 gagccaccat tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt
    10501 gatgagagaa cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca
    10561 gccagcgtac gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc
    10621 aaattgcggg gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta
    10681 tctctaaagg acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca
    10741 ttttccctta ttctgtagcg aaatagcacg atcatgacgc tatatacatg attgcatagc
    10801 gaaagtgtgg atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact
    10861 ctagcctctg cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga
    10921 ttgccaccag ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg
    10981 cttcacaact gactgatgta tttgcgcgta agttttacta cttgcgcctg tcgattaccg
    11041 atgtgtgtaa ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca
    11101 ccaataaagg ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc
    11161 tgggcaccga aaaagtgcgc ctgacaggag gagagccgtc tttacgccgc gactttaccg
    11221 atatcatcgc cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg
    11281 gttaccgtct ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg
    11341 tcagtgtcga cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca
    11401 accaggtcat ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca
    11461 ataccgtgct gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc
    11521 agcatcgccc tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc
    11581 tcttccgtaa gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct
    11641 ggatccacca attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt
    11701 acgccggaga gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc
    11761 gcctgcgcgt ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta
    11821 acctgcgcga tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag
    11881 cggcgctgcg ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc
    11941 aaaacttatc gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa
    12001 gcactgaatt tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag
    12061 aagacgatac ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg
    12121 tcgatgaagc cattgtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga
    12181 tcgccagcga cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg
    12241 atcaggctcc cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag
    12301 tgttccgtat gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag
    12361 cgggcgtcgc taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta
    12421 ccgcatggga aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc
    12481 atccacattt gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc
    12541 gcacatggtg gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt
    12601 tgtcaccatg cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga
    12661 cgtatttgcc actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat
    12721 cccgctgtgt cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga
    12781 gcacaatcgg gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat
    12841 ggaagcgtta accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt
    12901 gcaaaaagat atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg
    12961 tgactttaag gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg
    13021 tgggaacaga tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc
    13081 acctggctgc gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg
    13141 ccgtcaacca gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt
    13201 tcttcccgcc ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag
    13261 ccgttcagcg taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta
    13321 gtcaccttca ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc
    13381 ctcgaacact atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt
    13441 aaccgctggc cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc
    13501 gatgaaatcg tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg
    13561 cagtttatta tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg
    13621 gaaggcgacc gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag
    13681 ttttttgtta gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg
    13741 tttacgctgc gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata
    13801 tcttcaggag atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccctgggc
    13861 tggcttgcaa acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac
    13921 cgcatttgtt gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa
    13981 ccgtgtcttt ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc
    14041 gatgattcaa aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct
    14101 gacgggtctt acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac
    14161 tttcgtcgtt actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg
    14221 cgatttaagt ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc
    14281 gctggtcaac ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt
    14341 gattgtcttt gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca
    14401 gattgatacc cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt
    14461 gtatctggac ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta
    14521 attctttttc accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga
    14581 cacttaacaa gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat
    14641 ccataaatac aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat
    14701 gttttttcta aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga
    14761 ttttcagcac gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt
    14821 ggcaaccgca ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga
    14881 ttcaccacca atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa
    14941 ggatatcaac aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt
    15001 ggtggcgatt gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac
    15061 tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg
    15121 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa
    15181 tgcggcatta atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg
    15241 gccaggcatt atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag
    15301 tgaagagttg tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta
    15361 ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca
    15421 tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat
    15481 cgccgcttca tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat
    15541 cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat
    15601 aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac
    15661 accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc
    15721 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac
    15781 catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac
    15841 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
    15901 aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata
    15961 ccagtaatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata
    16021 gcccagccag ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa
    16081 ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt
    16141 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
    16201 accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac
    16261 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
    16321 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
    16381 ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt
    16441 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga
    16501 tcaacctgcg tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg
    16561 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc
    16621 atggtttcat ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc
    16681 gtctggttaa aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg
    16741 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
    16801 ccgtggagcg ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta
    16861 tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
    16921 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg
    16981 aagaagtagg cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa
    17041 tggcgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
    17101 tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg
    17161 ctctgtccag gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca
    17221 aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag
    17281 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
    17341 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac
    17401 actacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
    17461 ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc
    17521 agctgtttgc cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt
    17581 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
    17641 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
    17701 gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
    17761 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
    17821 ggcgtccgtg ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat
    17881 tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta
    17941 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
    18001 ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt
    18061 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa
    18121 tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac
    18181 cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
    18241 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
    18301 tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa
    18361 acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt
    18421 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
    18481 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga
    18541 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct
    18601 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
    18661 gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt
    18721 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
    18781 ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc
    18841 ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc
    18901 ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
    18961 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
    19021 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
    19081 tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt
    19141 aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt
    19201 gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
    19261 ccatcgtaaa gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac
    19321 caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc
    19381 cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt
    19441 cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg
    19501 cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta
    19561 ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt
    19621 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
    19681 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
    19741 aacgcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt
    19801 tcagcagcag cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg
    19861 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg
    19921 cggcgaacgg gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt
    19981 gtggttcgcg cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
    20041 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
    20101 accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg
    20161 aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg
    20221 cggatcaccg cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc
    20281 agaaaagcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa
    20341 atcagcagcc ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
    20401 agcagcgaac cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac
    20461 atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg
    20521 gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc
    20581 agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata
    20641 aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt
    20701 tcaaaagtct gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg
    20761 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
    20821 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga
    20881 ccgcgaattt tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg
    20941 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
    21001 tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa
    21061 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg
    21121 atctgccgcg tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta
    21181 ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt
    21241 gggatcgggt tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc
    21301 gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
    21361 gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa
    21421 ttcacggcgg gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg
    21481 ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc
    21541 ggtggcgtgt gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc
    21601 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
    21661 cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt
    21721 accggaactc tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag
    21781 caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc
    21841 aaaaatctcc ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
    21901 tttcttggtc agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
    21961 caatattgca cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc
    22021 atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg
    22081 aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat
    22141 gccatcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg
    22201 tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc
    22261 ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
    22321 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
    22381 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
    22441 tttcggttcg cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag
    22501 cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa
    22561 agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
    22621 catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
    22681 cagcgcgccg tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg
    22741 tttcagtaat cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac
    22801 caaccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc
    22861 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
    22921 cctcgtcacc gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg
    22981 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
    23041 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg
    23101 ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt
    23161 catcaacata agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac
    23221 cttcattgag aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg
    23281 ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt
    23341 cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca
    23401 atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt
    23461 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
    23521 tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt
    23581 cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg
    23641 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
    23701 ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga
    23761 aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
    23821 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc
    23881 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
    23941 gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
    24001 ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat
    24061 caacgcatgg gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc
    24121 aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta
    24181 ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc
    24241 ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc
    24301 ccgatcaggc tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg
    24361 ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
    24421 gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt
    24481 ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc
    24541 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
    24601 gattgattaa gattatgact ccatatggga gttgtccagt atggctaaga attttagcta
    24661 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
    24721 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
    24781 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
    24841 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
    24901 cctgctgtgc tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca
    24961 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    25021 cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc
    25081 gaaaacgtcc gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt
    25141 gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg
    25201 ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    25261 ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    25321 gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac
    25381 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    25441 cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    25501 cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc
    25561 cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt
    25621 gtggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa
    25681 tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa
    25741 gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt
    25801 atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat
    25861 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    25921 attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    25981 caacgtggcg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    26041 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    26101 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    26161 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    26221 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    26281 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    26341 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    26401 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    26461 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    26521 tgtgcctgaa aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg
    26581 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    26641 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    26701 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    26761 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct
    26821 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    26881 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    26941 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    27001 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    27061 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    27121 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct
    27181 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    27241 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    27301 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    27361 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    27421 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    27481 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    27541 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    27601 caccgtgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    27661 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    27721 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    27781 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    27841 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    27901 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    27961 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    28021 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    28081 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    28141 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    28201 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    28261 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    28321 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    28381 acaggtggag agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat
    28441 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    28501 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    28561 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    28621 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    28681 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    28741 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    28801 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    28861 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa
    28921 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    28981 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    29041 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    29101 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    29161 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    29221 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    29281 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    29341 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    29401 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    29461 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    29521 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag
    29581 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    29641 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    29701 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    29761 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    29821 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    29881 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta
    29941 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    30001 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    30061 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    30121 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    30181 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    30241 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    30301 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    30361 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    30421 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    30481 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    30541 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    30601 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    30661 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    30721 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    30781 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    30841 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    30901 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    30961 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    31021 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    31081 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    31141 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    31201 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    31261 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    31321 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    31381 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    31441 ggcggaagtg atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc
    31501 ttccagggca tcgagggggg atgcgccatc ggcagaaacc acgccgattt tattcatgtt
    31561 ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag
    31621 agccatagca gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt
    31681 tgtataagtg acgttgtttc gtcgatgtga tgagtatcac gtttttttac gagagataaa
    31741 atcgaagaga attgaccgcc ttgttcaaat aaattgattg atatctaaca tactgataaa
    31801 ccatttattt gttgtactta tggacttgtt ttatgccgcg tggcagtcac agcagcgtta
    31861 aaggcacgga atgccacgca aaatacaaat tatactcgct atgcatctcg ccagttgtag
    31921 ttaacgtaac tgtctgtctt tcgaacctct gcgattatat cctgtatacg ctggtttttg
    31981 taaatctttc tcctgctgac aatgaataca taagcgcacg ccaggaatgg cttcccgacg
    32041 ggcttgcggg atgggggcac cgcactcttc acattcatac aggctttcgc cacgcggaat
    32101 ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt cgttgacggc
    32161 gtcatcgtta gcccaagcgg atgccatatc gtcctcccca tatcaatgct tgtacagtta
    32221 agtgtagcta atatagggac gaacctgggc agttcaagcg tcagatctcc gaccattccc
    32281 gcagcagatt atgataaaga ttgagcagcg acaggatctc ttcattttca ccatagcggc
    32341 ttttcagcga ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc
    32401 ggatcatcga ctggatccac ataaatgatg ccactcgtac gccgcgggtt acgggtgtca
    32461 cgcaatgcag gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat
    32521 gttgtccaaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta
    32581 aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg
    32641 cgccatccac atggaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg
    32701 aaagggtacg cggcaaggca gcggcaaaga acaatgcatg ttggttaacc gcgttcagca
    32761 cctcattttg cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga ttgttcttaa
    32821 cttgtgcacc ttgtgctccg gtggtgacgc gtccatccac ccattcggct tgttccagtt
    32881 gttcgcgaaa atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac
    32941 aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt
    33001 gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttgt tgattgaggc
    33061 gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat tgcgattaac
    33121 ccgatacccc agtttggcat cggcgaccca gtaaccttct gtaaatgctg gcgttcccac
    33181 cgcgccgtct gaacctttat gtatactgcc gatatagcgt gcgcccgcgc caacagagat
    33241 atcatcggtt gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag
    33301 cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt
    33361 atagccgcca atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc
    33421 ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt
    33481 ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca
    33541 tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact
    33601 gttaccactg ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac
    33661 ggcatagtta atatagacat tgccgttttc cgtcaggtgg tacagcgccc cggctttcca
    33721 gttcaccaga ttgcccgact tggcggtgtc gacggtggtg actggagaac cttttgccac
    33781 accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata
    33841 ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg
    33901 cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt
    33961 gcgcgtcagg ccgccgggat gaattctgct gtcaggatga taaatattta ccgccggtaa
    34021 cgtcaccgga ttaacgccat agttagtctg cgtttcacgg gtaaattcca cgccggtact
    34081 gacatcatga ccgatagaac cggtatagaa cgtcgaggtc aggttggtct ggttggtgag
    34141 aattttatta ctcacatctt tggtattcgc cgtgcgtgac taggtccagc tattcacatc
    34201 gctggtgggc tgagtaatat tcgacgcccc gcccataatc gccgtcatca ggtaatcctg
    34261 ctttacgcgc gaccaacggg tagtattgcg aatggtggtg ttatcgttga tgtcgtgctc
    34321 aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa
    34381 gttatgagta tcaacttttc cggaatgatt cagggccgcc gctcccgcag atggggcaga
    34441 atagcccggc aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct gagtgacatg
    34501 cagataatta agatacaaac gatttgctgt accaaggcca aaagcgacag aaggggcgac
    34561 gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgggtttttt cgcccatcac
    34621 attcaggcgc accgcggtag tatcaccaat gacctgattg acgtccagcg tgccgcggcg
    34681 gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc gcggctgctt
    34741 gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc cggacggccc
    34801 tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg
    34861 aataccatca atataaatac tgttagaggt atcggcacca cgcatataaa tggcgtcgcc
    34921 agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgccgg
//
LOCUS       DAJVQR010000034        34904 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVQR010000034 DAJVQR010000000
VERSION     DAJVQR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            859..3144
                     /gene="nrdA"
                     /locus_tag="OQM52_001779"
     CDS             859..3144
                     /gene="nrdA"
                     /locus_tag="OQM52_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1563660.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3378..4508
                     /gene="nrdB"
                     /locus_tag="OQM52_001780"
     CDS             3378..4508
                     /gene="nrdB"
                     /locus_tag="OQM52_001780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1563661.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4508..4762
                     /gene="yfaE"
                     /locus_tag="OQM52_001781"
     CDS             4508..4762
                     /gene="yfaE"
                     /locus_tag="OQM52_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1563662.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4816..5466)
                     /gene="inaA"
                     /locus_tag="OQM52_001782"
     CDS             complement(4816..5466)
                     /gene="inaA"
                     /locus_tag="OQM52_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS1563663.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5645..5887
                     /locus_tag="OQM52_001783"
                     /pseudo
     CDS             5645..5887
                     /locus_tag="OQM52_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5929..7005)
                     /gene="glpQ"
                     /locus_tag="OQM52_001784"
     CDS             complement(5929..7005)
                     /gene="glpQ"
                     /locus_tag="OQM52_001784"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1563664.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7010..8368)
                     /gene="glpT"
                     /locus_tag="OQM52_001785"
     CDS             complement(7010..8368)
                     /gene="glpT"
                     /locus_tag="OQM52_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1563665.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8641..10269
                     /gene="glpA"
                     /locus_tag="OQM52_001786"
     CDS             8641..10269
                     /gene="glpA"
                     /locus_tag="OQM52_001786"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1563666.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10259..11518
                     /gene="glpB"
                     /locus_tag="OQM52_001787"
     CDS             10259..11518
                     /gene="glpB"
                     /locus_tag="OQM52_001787"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1563667.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11515..12705
                     /gene="glpC"
                     /locus_tag="OQM52_001788"
     CDS             11515..12705
                     /gene="glpC"
                     /locus_tag="OQM52_001788"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1563668.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12898..13848
                     /locus_tag="OQM52_001789"
     CDS             12898..13848
                     /locus_tag="OQM52_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1563669.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13889..14359)
                     /locus_tag="OQM52_001790"
     CDS             complement(13889..14359)
                     /locus_tag="OQM52_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1563670.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14414..15616)
                     /locus_tag="OQM52_001791"
     CDS             complement(14414..15616)
                     /locus_tag="OQM52_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS1563671.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15703..16245)
                     /locus_tag="OQM52_001792"
     CDS             complement(15703..16245)
                     /locus_tag="OQM52_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563672.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16524..16949
                     /gene="nudI"
                     /locus_tag="OQM52_001793"
     CDS             16524..16949
                     /gene="nudI"
                     /locus_tag="OQM52_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1563673.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16988..17590)
                     /locus_tag="OQM52_001794"
     CDS             complement(16988..17590)
                     /locus_tag="OQM52_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1563674.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17898..19037
                     /gene="arnB"
                     /locus_tag="OQM52_001795"
     CDS             17898..19037
                     /gene="arnB"
                     /locus_tag="OQM52_001795"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1563675.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19041..20009
                     /gene="arnC"
                     /locus_tag="OQM52_001796"
     CDS             19041..20009
                     /gene="arnC"
                     /locus_tag="OQM52_001796"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1563676.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20009..21990
                     /gene="arnA"
                     /locus_tag="OQM52_001797"
                     /pseudo
     CDS             20009..21990
                     /gene="arnA"
                     /locus_tag="OQM52_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21987..22877
                     /locus_tag="OQM52_001798"
     CDS             21987..22877
                     /locus_tag="OQM52_001798"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1563677.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22877..24529
                     /gene="arnT"
                     /locus_tag="OQM52_001799"
     CDS             22877..24529
                     /gene="arnT"
                     /locus_tag="OQM52_001799"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1563678.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24526..24861
                     /gene="arnE"
                     /locus_tag="OQM52_001800"
     CDS             24526..24861
                     /gene="arnE"
                     /locus_tag="OQM52_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1563679.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24861..25247
                     /gene="arnF"
                     /locus_tag="OQM52_001801"
     CDS             24861..25247
                     /gene="arnF"
                     /locus_tag="OQM52_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1563680.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25241..25513)
                     /gene="pmrD"
                     /locus_tag="OQM52_001802"
     CDS             complement(25241..25513)
                     /gene="pmrD"
                     /locus_tag="OQM52_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1563681.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25618..26973)
                     /gene="menE"
                     /locus_tag="OQM52_001803"
     CDS             complement(25618..26973)
                     /gene="menE"
                     /locus_tag="OQM52_001803"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1563682.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26970..27932)
                     /gene="menC"
                     /locus_tag="OQM52_001804"
     CDS             complement(26970..27932)
                     /gene="menC"
                     /locus_tag="OQM52_001804"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1563683.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27932..28789)
                     /gene="menB"
                     /locus_tag="OQM52_001805"
     CDS             complement(27932..28789)
                     /gene="menB"
                     /locus_tag="OQM52_001805"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1563684.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28804..29562)
                     /gene="menH"
                     /locus_tag="OQM52_001806"
     CDS             complement(28804..29562)
                     /gene="menH"
                     /locus_tag="OQM52_001806"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1563685.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29559..31229)
                     /gene="menD"
                     /locus_tag="OQM52_001807"
     CDS             complement(29559..31229)
                     /gene="menD"
                     /locus_tag="OQM52_001807"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1563686.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31318..32613)
                     /gene="menF"
                     /locus_tag="OQM52_001808"
     CDS             complement(31318..32613)
                     /gene="menF"
                     /locus_tag="OQM52_001808"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1563687.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32692..32997)
                     /gene="elaB"
                     /locus_tag="OQM52_001809"
     CDS             complement(32692..32997)
                     /gene="elaB"
                     /locus_tag="OQM52_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1563688.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33052..33513)
                     /locus_tag="OQM52_001810"
     CDS             complement(33052..33513)
                     /locus_tag="OQM52_001810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1563689.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33578..34495
                     /gene="rbn"
                     /locus_tag="OQM52_001811"
     CDS             33578..34495
                     /gene="rbn"
                     /locus_tag="OQM52_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS1563690.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34686..34874
                     /locus_tag="OQM52_001812"
                     /pseudo
     CDS             34686..34874
                     /locus_tag="OQM52_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8537 a         9340 c         8649 g         8378 t
ORIGIN      
        1 gcgtaatgcc tgaaaggctt tgcctgctgg cgtgacaact cgctttgata tcatatccct
       61 ggcataaatc aatggccgcc gcgacaccgt tagcagagaa aagagatgca atcattgacg
      121 ggagtagaga taaatactcc ttgcgtagaa agataatccg catattaatc tgaccatcat
      181 tttatataag acggcatatt taaacgcatt atacaatatg ggttatcttt cttttgtaaa
      241 ttgtccaacc cgatgcaatg gctattttgt tattatttga tgaataatat cagtgcgtca
      301 taattcaagt taataacctt aagggatacc agttatattt aaactaaatt aaagtaatga
      361 ataattttct tataatataa ggtaaattaa caaaatggct tcgcatttaa caataaccga
      421 atagaaaaca accatttcga catcaacaat cgcttacatt cgcttatata ttgaccacaa
      481 ctgatacatc agattatgtg atgactcgtg cttagatcaa tttttgcaat cattagcaaa
      541 aagattaata aaccatctat atcaatttat ccaacctatt ctgccgttca agaaatcgcc
      601 gaacagttat ttttaacaaa tttttctctt cccattgact ttcccggaca ccttgtctga
      661 cctaaggtgc gcgaaagcca ctttttcctt cctgagttat ccacaaagtt atgcacttgc
      721 aagagggtca ttttcacact atcttgcagt gaatcccaaa cataccccct atatatagtg
      781 ttctaagcag cttcccttac tacaggtagt ctgcatgaaa ctattgcgga aagaattcca
      841 aaaacaggta cgacatacat gaatcagaat ctgctggtga caaagcgcga cggtagcaca
      901 gagcgcatca atctcgacaa aatccatcgc gttctggatt gggcggcaga aggactgcat
      961 aacgtttcga tttcccaggt cgagctgcgc tcccacattc agttttatga cggtatcaag
     1021 acctctgaca tccacgaaac tattatcaag gctgccgcag acctgatctc ccgtgatgcg
     1081 ccggattatc agtatctcgc cgcgcgcctg gcgatcttcc acctgcgtaa aaaagcctac
     1141 ggccagtttg agccgcctgc gctgtacgac cacgtggtga aaatggtcga gatgggcaaa
     1201 tacgataatc atctgctgga agactacacg gaagaagagt tcaagcagat ggacacgttt
     1261 atcgatcacg accgtgatat gaccttctct tatgctgccg ttaagcagct ggaaggcaaa
     1321 tatctagtac agaaccgcgt gaccggcgaa atctacgaaa gcgcccagtt cctttatatt
     1381 cttgttgccg cgtgcctgtt ctcgaactac ccgcgtgaaa cgcgcctgca atacgtgaag
     1441 cgtttttatg acgcggtttc cacatttaaa atttcgctgc cgacgccaat catgtccggc
     1501 gtgcgtaccc cgactcgtca gttcagctcc tgcgtactga tcgagtgcgg tgacagcctg
     1561 gattccatca acgccacctc cagcgcgatt gtgaaatatg tttcccagcg tgccgggatc
     1621 ggcatcaacg ccggacgtat tcgtgcgctg ggcagcccga ttcgcggtgg cgaagcgttc
     1681 cataccggct gtatcccgtt ctacaaacat ttccagacag cggtgaaatc ctgctctcag
     1741 ggcggtgtgc gcggcggtgc ggcaacgctg ttctacccga tgtggcatct ggaagtggaa
     1801 agcctgctgg tgttgaaaaa caaccgtggg gttgaaggca accgcgtgcg tcatatggac
     1861 tacggggtac aaatcaacaa actgatgtat acccgcctgc tgaaaggtga agatatcacc
     1921 ctgttcagcc cgtccgacgt accggggctg tacgacgcgt tcttcgccga tcaggaagag
     1981 tttgaacgcc tgtataccaa atatgagaaa gacgacagca tccgcaagca gcgtgtgaaa
     2041 gccgttgagc tgttctcgct gatgatgcag gaacgtgcgt ctaccggtcg tatctatatt
     2101 cagaacgttg accactgtaa tacccatagt ccgtttgatc cgaccatcgc gccagttcgc
     2161 cagtctaacc tgtgcctaga aattgccctg ccgaccaaac cgctgaacga cgtcaacgac
     2221 gagaacggtg aaatcgcgct gtgtacgctg tctgctttca acctgggcgc aattaatagc
     2281 ctggatgaac tggaagagct ggcgattctg gcggttcgtg cacttgacgc gctactggat
     2341 tatcaggatt acccgatccc ggccgccaaa cgtggagcga tggggcgtcg tacgctgggt
     2401 attggtgtga ttaacttcgc ttactatctg gcgaagcacg gtaaacgcta ctccgacggc
     2461 agcgccaaca acctgacgca taaaaccttc gaagcgattc agtattacct gctgaaagcg
     2521 tctaatgagc tggctaaaga gcaaggcgcg tgcccgtggt ttaacgaaac cacttacgcg
     2581 aaagggatcc tgccgatcga tacctataag aaagatctgg atgccatcgc taatgagccg
     2641 ctgcattacg actgggaagc attgcgtgaa tcaatcaaaa cgcacggcct gcgtaactcc
     2701 acgctttctg ccctgatgcc gtccgagacc tcttcgcaga tctctaacgc cactaacggt
     2761 attgaaccgc cgcgcggtta cgtcagcatc aaagcgtcga aagacggtat tttgcgccag
     2821 gtggtgccgg actacgagca cctgcacgac gcctatgagc tgctgtggga aatgccgggt
     2881 aacgatggct acctgcaact ggtaggtatc atgcagaaat ttatcgatca gtcgatctct
     2941 gctaacacca actacgatcc gtcacgtttc ccgtcaggaa aagtgccgat gcagcagttg
     3001 ctgaaagacc tgctcaccgc ctacaaattc ggggtcaaaa cgctgtatta tcagaacacc
     3061 cgtgacggcg ctgaagacgc gcaggacgat ctggtaccgt caatccagga cgatggctgc
     3121 gaaagcggcg catgtaagat ctgatattga gatgccggat gcggcgtaaa cgccttatcc
     3181 ggcctacggc tcggtttgta ggcctgataa gacgcgccag cgtcgcatca ggctccgggt
     3241 gccggatgca gcgtgaacgc cttatccggc ctacggctcg gatttgtagg cctgataaga
     3301 cgcgccagcg tcgcatcagg cacaggatgc ggcgtaaaat gccttatccg gcattaaact
     3361 cccaacagga cacactcatg gcatatacca ccttttcaca gacgaaaaat gatcagctca
     3421 aagaaccgat gttctttggt cagccggtca acgtggctcg ctacgatcag caaaaatatg
     3481 acatcttcga aaagctgatc gaaaagcagc tctctttctt ctggcgtccg gaagaagttg
     3541 acgtctcccg cgaccgtata gattaccagg cgctgccgga gcacgaaaaa cacatcttta
     3601 tcagcaacct gaaatatcag acgctgctgg attccattca gggtcgtagc ccgaacgtgg
     3661 cgctattgcc gcttatttct attccggaac tggaaacctg ggtcgaaacc tgggcgttct
     3721 cagaaacgat tcattcccgt tcctatactc atatcattcg taatatcgtt aacgatccgt
     3781 ctgttgtgtt tgacgatatc gtcaccaacg agcagatcca gaaacgtgcg gaagggatct
     3841 ccagctatta cgatgaactg atcgaaatga ccagctactg gcaccttctg ggcgaaggca
     3901 cccacaccgt taacggtaaa accgtaaccg ttagcctgcg cgagctgaag aaaaaactgt
     3961 atctctgcct gatgagcgtt aacgcgctgg aagcgatccg cttctacgtc agctttgctt
     4021 gttcctttgc ctttgcagaa cgcgaactga tggaaggtaa cgccaagatt attcgcctga
     4081 ttgcccgcga tgaagccctg cacctgaccg gtactcagca tatgctgaat ctgctgcgca
     4141 gcggcgcgga tgatccggag atggcggaaa ttgccgaaga atgtaagcag gagtgctatg
     4201 acctgtttgt ccaggcggca cagcaagaga aagactgggc ggattatctg ttccgtgacg
     4261 gttcgatgat tggtctgaat aaagatattc tctgccagta cgttgaatac atcaccaata
     4321 tccgcatgca ggcagtcggt ttggatctgc cgttccagac gcgctccaac ccgatcccgt
     4381 ggatcaacac ctggctggtg tctgataacg tgcaggttgc tccgcaggaa gtggaagtca
     4441 gttcttatct ggtcgggcag attgacgcgg aagtggacac cgacgatttg agtaacttcc
     4501 agctctgatg gcccgcgtta ccctgcgcat cactggcaca caactgctgt gccaggatga
     4561 acacccttcc cttctggcgg cgctggaatc ccacaatgtg gcggttgagt accagtgccg
     4621 cgaaggttac tgcggctcct gtcgcacccg actggtcgca ggtcaggtgg actggattgc
     4681 cgaaccgtta gcctttattc agccggggga aattttgccc tgttgttgcc gggcaaaagg
     4741 cgatattgaa atcgagttgt gatcgggtac tagcgccaga tacgacgctt acgcgtatta
     4801 tctggcctac aacgattaca tggcgtagta atacgctttc acctgctccc agtccgcttt
     4861 agggatcggc tccagatatt tttccagctg gcggaagtca tgattaatcg ctttatcgcg
     4921 acgcaagcgg cgacgacttt tctccagatc aagaaaacca gcttctgcct taccttctgt
     4981 tttcacatag atatggcgaa catagcaaca gccatgctga cgattaacgc tatgcatttt
     5041 cttaaacgcc agtgccactg ctttcaacat ggcttgccgt acttcgtcag aataaggcga
     5101 tactgcatgc cgggcatacc agtcagcaat gctgatgaac cccgccatat cttcagtcac
     5161 cagcaacgcg cgccattcac cctcaatttt caccgcttca ccaaaaacga tcttcggcac
     5221 gatgacaccg gcccgttcca gttctttaat taccgcaacc tcacggacaa tcgttggtcg
     5281 gccgaacgga taacgtactg aatgaaacag atgatgcgtc atgcgcttta catacagctt
     5341 tttgccgttg cgctcgacgc attgcacccc gctcatacca ttacggcgat agttaggctc
     5401 ttcaacccag tcgccctctg ttgcccacca gtgattaaat tcgtcgtact ttgctgaaac
     5461 tgccataccc tatcgcctgt catttttatt aacgacaatg actataggtg gttacctgag
     5521 gaaaatctta atgaaacgtg tcgtattaat gaatttcttt tgccgctaca ggtgtgacac
     5581 cgcttttgcc cttcatttgc agcgcataga ttccaccagc cactaccact aatagtgcgc
     5641 ccagcagata aaatgtgaat ttaatcagtc ggatacagcg atgaatttta ttcgacaggg
     5701 attaggcatc gccttacaac cagagttaac gctgaaaagc attgcaggtg aattgtgttc
     5761 cgttcctcac gaaccaacct tctatcgaca gatttcgttg ctgactaaag aaaagccggt
     5821 agaaggcagt ccactgtttt tactacaaat gtgcatggaa caattagtgg cgattggaaa
     5881 aatttgatat gagacaggat ggcgcatgaa cgccatcctg cagtaacatt actctttatt
     5941 aagaaatttt actgccttat cagggaaatc agtaaacagc ccatttacac ccgctttgtt
     6001 atacagagca tcatataact gattcacatc aggagtgtat tcaggcagtt tatctgaccg
     6061 cacggtataa ggatgcacta ccagtttatt ctgctgggca tcttgcacca tgccagtgag
     6121 tttgatatta cccggctgcg atgtctcctc aatcaacata tggtaatccg gaccaatacc
     6181 atctgcatat tccgccacct gtttcatggc tcccggctta aacatccagt cgtagttgta
     6241 attaacccag cttccatccg gctgtttctg ctgcgtttca ttccagtcgg tataggcaat
     6301 cagctgtacc agattgagct ccatgcccat tttgggttcc agctcattct taatacgctt
     6361 cagctcatca gcatcaaaac attgcaaata aactttatcg tctttaccgg tgtaaccata
     6421 tttcttcagc acttccagcg tttttgccgc aatatccttc ccttcctgat gatggaacca
     6481 cggcgctttg atttctggat agataccgat atttttcccg gtagagtgat ttaacccctg
     6541 aacaaattca atctcttctt caaaggtgtg cacccggaag tcggacttac ccattgggaa
     6601 acgccccgga taagtctgta cttttttacc gttttcaata tcgaaacctt cggtaaattt
     6661 cagcgactta atttcatcca gcgtgaaatc tatcgcgtag taacgaccgt ctttgcgcgc
     6721 ccgatccggg aaacgatcgg caacatcagt aacacgatcg agataatggt catgcagaac
     6781 aaccagatgg tcgtctttgg tcatcaccaa atcctgttcc agataatccg ctccctgcgc
     6841 atacgccatc gcttttgctg gcagcgtatg ctccggcaaa tatccactgg caccgcgatg
     6901 ggcgatgact attttttcgt tgctgtccgc cgccattgca ctgcttccca tgactatagt
     6961 gctcatcatg atcgccatgc taaggttttt cagcttcaat ttcatgccat tagcctccgt
     7021 tgcgtttttg cagtaattgt tcatggcgac gtttttcgcc aatcatcaca acaatcaaca
     7081 agataaccgc cagaatgctg ccgccaatca ttaccataaa gccgccatcc cagccgaaga
     7141 agtccacggt gtagccaaca atcgcgctcg ccgccaccga accgcccagg taaccaaaca
     7201 gcccggtaaa gcccgctgcc gtacctgccg cttttttcgg tgccagttcc agcgcatgca
     7261 gaccgatcag catcacagga ccgtagatca ggaagccgat aacaatcata caaatcatat
     7321 cgacggttgg gttacctgcc gggttcatcc agtaaacgat agtcgcgatg gtcaccagtg
     7381 tcataaagaa gacgccggtt gccccacggt taccacggaa gactttgtcc gacatccagc
     7441 cgcacagcag ggtgcccgga atacccgcat actcatagaa gaagtaggcc caggaggatt
     7501 tatccagcgc gaaatgctta acctctttca gataagtcgg tgaccagtcg aggatgccgt
     7561 aacgcagcag ataaacgaac acgttggcga tggcgatgta ccacagcagt ttgttcggca
     7621 gcacgtactg catgaagatt tgcttcgcgg tcagctcctg ttccgctttt tcgttatagt
     7681 cgtccggata atcatttttg tactcttcga tcggcggcaa gccacaggat tgcggggtat
     7741 cgcgcatcat cgcaaaggcg aataatgcca ccagaatggc gcagaaagca ggcatataga
     7801 gcgccgcatg ccagtcattg aaccaggcca tccccagcag gaacagcagc ggcggaatac
     7861 caccaccgac gttgtgcgca cagttccaca ctgacacaat gccgccacgt tctttctgcg
     7921 accaccagtg caccatagta cgaccacacg gcggccaccc catcccctgg aaccaaccgc
     7981 agaggaacaa cagtacaaac atcaccgcaa tgctcgacgt cgcccatggc acaaagccca
     8041 taaacaacat cactgccgcc gccagaatca aacctgcggg caggaaaacg cgcggattcg
     8101 agcgatccga taccgaaccc atgatgaatt tcgaaaatcc ataagcaatc gagatccccg
     8161 aaagggcaaa acctaaatca ccgcgtgaga atccctgctc aaccagataa ggcatagcaa
     8221 gcgcaaagtt cttacgaacc aaatagtaag ccgcatagcc aaagaatatc cccaggaaaa
     8281 tttgccagcg caatcgacga taagtcggat cgatctccgc ggcaggtaag cgcgctttgt
     8341 gtggcgctgg tttaaaaata ctcaacattg atagcctccg tggcccgtgg tcttatttat
     8401 gattaacagc ctgattcagt gagagaacct gccgtttctt gagttgccgc gatgttaaga
     8461 aaacattcat aaattaaatg tgaattgccg cacacattat taaataagat ttacaaaatg
     8521 ttcaaaatga cgcatgaaat cacgttttac tttcgaatca tgagcgaata tgcgcgaaat
     8581 caaacaattc atgtttttac tatggctaaa tggtaaaaaa cgaacttcag agggataaca
     8641 atgaaaactc gcgactcgca atcaagtgac gtgattatca ttggcggcgg cgcaacggga
     8701 gccgggattg cccgcgactg tgccctgcgc gggctgcgcg tgattttggt tgagcgccac
     8761 gacatcgcaa ccggtgccac cgggcgtaac cacggcctgc tgcacagcgg tgcgcgctat
     8821 gcagtaaccg atgcggaatc ggcccgcgaa tgcattagtg aaaaccagat cctgaaacgc
     8881 attgcacgtc actgcgttga accaaccaac ggcctgttta tcaccctgcc ggaagatgac
     8941 ctctcattcc aggccacttt tattcgcgcc tgcgaagaag cagggatcag cgcagaagct
     9001 atagacccgc agcaagcgcg cattatcgaa cctgccgtta acccggcact gattggcgcg
     9061 gtgaaagttc cggatggcac cgttgatcca tttcgtctga ccgcagcaaa catgctggat
     9121 gccaaagaac acggtgccgt tatccttacc gctcatgaag tcacggggct gattcgtgaa
     9181 ggcgcgacgg tgtgcggtgt tcgtgtacgt aaccatctca ccggcgaaac tcaggccctt
     9241 catgcacctg tcgtggttaa tgccgctggg atctgggggc aacacattgc cgaatatgcc
     9301 gatctgcgca ttcgcatgtt cccggcgaaa ggatcgctgc tgatcatgga tcaccgcatt
     9361 aaccagcatg tgatcaaccg ctgccgtaaa ccttccgacg ccgatattct ggtgcctggc
     9421 gataccattt cgctgattgg taccacctct ttacgtattg attacaacga gattgacgat
     9481 aatcgagtga cggcagaaga ggttgatatt ctgctgcgtg aaggggaaaa actggccccc
     9541 gtgatggcga aaacgcgcat tttgcgggcc tattctggcg tgcgcccgct ggttgccagc
     9601 gatgacgacc cgagcggacg taacgtcagc cgtggcatcg tgctgctcga ccatgctgaa
     9661 cgcgatggtc tggacggatt tatcaccatc accggtggca aactgatgac ctatcggctg
     9721 atggctgaat gggctaccga cgcggtatgc cgcaaactgg gcaacacgcg cccctgtacg
     9781 actgccgatc tggcactgcc tggttcacaa gaacccgctg aagttacctt gcgtaaagtc
     9841 atctccctgc ctgccccgct gcgcggttct gcggtttatc gtcatggcga tcgcacgcct
     9901 gcctggctga gcgaaggccg tctacaccgt agcctggtat gtgagtgcga agcggtaact
     9961 gcgggtgaag tgcagtacgc ggtagaaaat ttaaacgtta atagcctgct ggatttacgc
    10021 cgtcgtaccc gtgtggggat gggcacctgc cagggcgaac tctgcgcctg ccgcgctgcc
    10081 ggactgctgc aacggtttaa cgtcacgacg tccgcgcaat ctatcgagca actttccacc
    10141 ttccttaacg aacgctggaa aggcgtgcaa cccatcgcct ggggagacgc actgcgcgaa
    10201 agcgaattta cccgctgggt ttatcaggga ctgtgtggtc tggagaagga gcagaaagat
    10261 gcgctttgat actgtcatta tgggcggcgg cctcgccgga ttactctgtg gcctgcaact
    10321 gcaaaaacac ggcctgcgct gtgccattgt cacgcgtggt caaagcgcgt tgcatttctc
    10381 atccggttcg ctggatttgc tgagccatct gccagatggt caacccgtgg cagacattca
    10441 cagtggactg gaatctttgc gtcagcaggc acctgcacat ccttactctc ttctcgggcc
    10501 acaacgcgtg ctcgatctcg cttgccaggc gcaggcatta atcgctgaaa gcggtgcgca
    10561 attgcagggc agcgtagaac tggctcacca gcggattacg ccgctcggca ctttgcgtgc
    10621 aacctggtta agttcgccag aagttcccgt ctggccgcta cccgcgaaga agatatgtgt
    10681 agtggggata agcgggctga tggatttcca ggcgcatctt gcggcggctt cgttgcgcga
    10741 actggatctt tcggttgaaa ccgcagaaat agagctgccg gaactggatg tcctgcgcaa
    10801 taacgccacc gaatttcgcg cggtgaatat cgcccgtttc cttgataatg aagaaaactg
    10861 gccgctgtta cttgatgcgc ttattcctgt cgccaatacc tgcgaaatga tcctgatgcc
    10921 cgcctgcttc ggcctggccg atgacaaact gtggcgttgg ttgaatgaaa aactaccttg
    10981 ttcactgatg cttttgccaa cgctgccgcc ttccgtgctg ggcattcgtc tgcaaaacca
    11041 gttacagcgc cagtttgtgc gccaaggtgg agtgtggatg ccgggcgatg aagtgaaaaa
    11101 agtgacctgt aaaaatggcg tagtgaatga aatctggacc cgcaatcacg ccgatattcc
    11161 gctacgtcca cgtttcgcgg ttctcgccag cggcagtttc tttagtggcg gactagtagc
    11221 ggaacgtaac ggcattcgcg agccgattct cggccttgat gtgctacaaa ccgccacgcg
    11281 gggtgaatgg tataagggcg atttttttgc gccgcaaccg tggcagcagt tcggtgtaac
    11341 cactgatgag acgctacgcc cgtcgcaggc agggcaaacc attgagaacc tgtttgccat
    11401 cggttcggtg ctgggcggat ttgatcccat cgcccaggga tgcggcggcg gtgtttgtgc
    11461 cgtcagtgct ttacatgccg ctcaacagat tgcccaacgc gcaggaggcc aacaatgaac
    11521 gacaccagct tcgaaaactg cattaagtgc accgtctgca ccaccgcctg cccggtgagc
    11581 cgggtgaatc ccggttatcc agggccaaaa caagccgggc cggatggcga gcgtctgcgt
    11641 ttgaaagatg gcgcactgta tgacgaggcg ctgaaatatt gcatcaactg caaacgttgt
    11701 gaagtcgcct gcccgtccga tgtgaagatt ggcgatatta tccagcgcgc gcgggcgaaa
    11761 tatgacacca cgcgcccgtc gctgcgtaat tttgtgttga gtcataccga cctgatgggt
    11821 agcgtttcca cgccgttcgc accaatcgtc aacaccgcta cctcgctgaa accggtgcgg
    11881 cagctgcttg atgcggcgtt aaaaatcgat catcgtcgca cgctaccgaa atactccttc
    11941 ggcacgttcc gtcgctggta tcgcagcata gcggctcagc aagcacaata taaagaccag
    12001 gtcgctttct ttcacggctg cttcgttaac tacaaccatc cgcagttagg taaagattta
    12061 attaaagtgc tcaacgcaat gggtaccggt gtacaactgc tcagcaaaga aaaatgctgc
    12121 ggcgtaccgc taatcgccaa cggctttacc gataaagcac gcaaacaggc aattacgaat
    12181 gtagagtcga tccgcgaagc tgtgggagta aaaggcattc cggtgattgc cacctcctca
    12241 acctgtacat ttgccctgcg cgacgaatac ccggaagtgc tgaatgtcga caacaaaggc
    12301 ttgcgcgatc atatcgaact ggcaacccgc tggctgtggc gcaagctgga cgaaggcaaa
    12361 acgttaccgc tgaaaccgct gccgctgaaa gtggtttatc acactccgtg ccatatggaa
    12421 aaaatgggct ggacgctcta caccctggag ctgttgcgta acatcccggg gcttgagtta
    12481 acggtgctgg attcccagtg ctgcggtatt gcgggtactt acggtttcaa aaaagagaac
    12541 taccccacct cacaagccat cggcgcacca ctgttccgcc agatagaaga aagcggcgca
    12601 gatctggtgg tcaccgactg cgaaacctgt aaatggcaga ttgagatgtc cacaagtctt
    12661 cgctgcgaac atccgattac gctactggcc caggcgctgg cttaaactcc cttctgatgc
    12721 ccggtaagca tgtggttacc gggcattttt gcgtacacga ttccgtgccc aatgtatgcg
    12781 ttgcaacgca gtgaaaattc ctctgaaaac gtctcgcaaa ggctgaaact ggcagatgtc
    12841 aaaggcctgg gataaccgta atgtcgcgtc atcataaata tcaggtgacg gacaaccatg
    12901 accgaatcaa caacctcctc cccgcatgat gcggtattta aaacctttat attcacaccc
    12961 gaaaccgcac gggattttct cgaaatacat ttaccagaac cactgcgcaa gctttgcaac
    13021 ctgcaaacct tacgcctgga acccactagt tttattgaaa aaagtttacg cgcttactac
    13081 tcggatgttt tgtggtccgt ggaaaccagc gacggtgacg gctatatcta ctgcgtgatt
    13141 gaacatcaaa gctctgcaga aaagaatatg gcttttcggc caatgcgcta tgccactgcc
    13201 gccatgcaga gtcacctaga taaaggctat gacagagttc cgctggtggt gccattgctg
    13261 ttttatcatg gcgaaacatc gccctacccg tactcactta actggctgga tgagtttgac
    13321 gatccgcaac ttgcccggca gttgtacacc gaagcttttc cgttggtgga tatcaccatc
    13381 gtacctgacg atgagatcat gcaacatcgg cgtatagctc tgctggaact gattcaaaag
    13441 catattcgcg accacgattt aatcggcatg gtcgacagga tcaccacgct tttggttaga
    13501 ggcttcacta atgacagcca gctacaaaca ctgtttaatt atctgctgca atgcggcgat
    13561 acctcccgtt tcacccgttt tattcaggag attgccgaac gttcaccact acaaaaggag
    13621 agattaatga ctattgctga acggctacgg caggaaggac atcaaattgg ctggcaagaa
    13681 ggtaaattag taggtttgca gcaaggtaaa ttagaaggtt tgcaggaagg tatgcatgaa
    13741 caagccatta aaattgcttt gcgcatgctg gaacagggca ttgatcgtga ccaagtgctc
    13801 gcggccaccc agctaagcga agccgatctg gcagcgaata accactaatt aacacaggcc
    13861 cacagccgat ccccatgggc ctttgatatc aataactacc ttttatgcgt ggcccatttt
    13921 tgcctgattt aaacatcgcc agtcgttgat ccagggcatc gctgtagagc atcgtgtcaa
    13981 cgccaacagc gacaaagttc gctccccacg ccaggcattg ctgcgccata tcaggagcca
    14041 cagccagaaa accagccgct ttacccgcag cacggatccg ccgaatgctg gtttcaataa
    14101 ttcgctgcac ttccgggtgc ccggcgttat ccgggtagcc caacgacgca gaaagatccg
    14161 ctggtccaat aaacacgcca tcaatccctt cgacgtcgag gatctcgtcc aggttatcca
    14221 gtgccgtttt actttccacc tgcactaaca gacaaagcga atcgttgact tgcgccatgt
    14281 aattctcaat gcgtccccag cgcgcagccc gtgccacact ggccccaaca ccacgctcac
    14341 cgtagggagg gttggacaaa ttttgccgga gggggaatgt gccggtgcag taaaccggca
    14401 cagagtggcg aaatcagacg gataaagtca tggactcaac aacctcaatc cagccatgct
    14461 cactagcgat gtcctggccg tttaaccaac ggcgtagcat attcagtgcc atcattgcgc
    14521 acacttcctg acggatagcc aggctgtagc gtgtagtgct gaaacgcaca cgcagagcga
    14581 aagtgccgtc tggcgtggct agcgcaaagt tgagatgctc gttctcgaaa cccgaaacag
    14641 ccagtgccag cccggcaaaa tggttggccc gccgttctgt aatccagtgc gcagtttgcg
    14701 ccagggtttc ttcctgtgaa ggaatcactt cacacgccag caatggagca cctgcgcgag
    14761 aaagttgcaa agccaataaa ccaccggtga attgctcgct caacgtcagg ctgaactggc
    14821 ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc
    14881 tctgtccggc aacacgttta acgtccagcc acagtttttc catcgcctgt tgctcgcttg
    14941 ccggtccggt gagtttcagt tcgatgatcg gcattgagga gcgatagccc attgttacgc
    15001 ccggcggcag ttgtagagtg tccaggcttt gtgccagatc gctttccgaa cgaccaaagg
    15061 tagttaaacg cagacaaacc ggcggctgtg gtaaagaaaa acgctcgcgc aggcgcggca
    15121 ggatttcgtg ctcgaccatc accttaaatt ctgacggtac gccgggagta aagaacatca
    15181 ggcaacgatt aagctgcacg gcaaaaccac aggcggtgcc taccgggtta ttgataaatt
    15241 cagcactggc aggcagctcc gcttgtttac ggttgctcgg tgccattact cgtccacgtt
    15301 cgtggaaata gcgttccatc tctttgagcc aggcttcatg cagcaccagg ccttcacctt
    15361 ttgctgtcgc agcggcgagt gcgcttaaat catcgctggt cggtcccagc ccgccgttaa
    15421 cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt
    15481 tatcccccac cgtattgcgg cgagataatg gcagcccctg atgaaagaaa aaatcggcca
    15541 gccaggcagc gttagtgtca acgatttgcc cgtataacac ttcatccccg gtggatagca
    15601 tttccacttt taacatggtt atctcctgct tttgttatcg aatcactata acgcaaaggg
    15661 agataaggcg tagtaagaaa ctgaatgccg cgccggaagg gatcagaaac tggcgttaac
    15721 cccaacatac aggccgtcag ccacagcgtt gtcgcggtta ccgtctttac ccgacagatt
    15781 caggtagcga taacccgcct caatactgac tggacgcata atggtgtaac gcgcgccagc
    15841 attcgcttcc tcataacttt gaataccgct ggagagcgaa tccggagagt agtaatactc
    15901 gccaaacaaa cggaagctgt tgccaatttt ccactgcaaa ccacctccta ccgctgcggc
    15961 ataaccttca tcgccgtaat tcgggttggt gtacacgcct tttccgccaa cggtcgccat
    16021 taacggcccg agaggcagat tcaaccccag ccccacgccc gcgacgtcac cgtcgtcgtc
    16081 gttatgtgtc cagttaccgc ttaaagccag gcccgtcgat tcagtcccaa aaccgacacc
    16141 aatattggtg tattctttac ccgcctggcc gctgatgctc attgcattga ccgatgcaga
    16201 aaccaacagc attccggcca gacctgctag cgcaattttt ttcattatcc cctttcccac
    16261 aagcaattaa tcaaaattcc caaagctgcg ggattgtact gcctggcaca gaggattcaa
    16321 tgagcgaaaa aagggatcta gtcacttatt gtaactaaat acttcttaaa taattaaacg
    16381 ccgaaaaaat aaacacactt taccgataca cacaaaaata ataataagtg cagttatcat
    16441 tgcaaaagag attactgtca ctttcacata tttctgagtt accgtattct catggtttct
    16501 ttttatttat aaggaatacc cctgtgcgac aacggactat tgtatgccct ttgattcaaa
    16561 atgatggtgc ttatttgctg tgcaaaatgg ccgacgatcg cggcgttttc cccggtcaat
    16621 gggcgctttc gggtggcggc gtggagtctg gcgaacgaat tgaagaggca ctacgccgcg
    16681 aaattcgcga agaactggga gaacagctgc ttttgacaga aatcacgccg tggaccttca
    16741 gcgatgatat tcgcaccaag acatatgcag atggtcgcaa ggaagagatt tatatgattt
    16801 acctgatttt tgactgcgtt tctgccaacc gagaagtgaa aataaacgaa gagtttcagg
    16861 actacgcgtg ggtaaaacct gaagatctgg tgcattatga tttaaatgtt gccacccgaa
    16921 aaacgttacg tttgaaaggt cttctttaac aatgatgaca gccatcactg gctgtcagat
    16981 tgaaaattta gtggttaacg aattccccat ccagataaac tttgcctttt tccacatgca
    17041 tgactaaacc atccagataa tcaggtttaa atatcgcatc acgcttattt ttagcaatat
    17101 atgtcaggca atgattatgg gtgaaaataa cgatattttt atcaggcgct ttgctttgta
    17161 agtccttaat tgcactataa atctcattac cgcactgaag aagtcgttta tctaccgtca
    17221 gctttttacc cgcggaaaac caggtcgccg actgaatggt ccggacggta ttactggaat
    17281 aaagatcgaa atcagagatg tcagcgctaa aagcgttgcc cagttcacgg gcatcctggg
    17341 tacctttaac cgtaatacct gttttatctg acaagcattg attggttgaa cgatcgcaac
    17401 gttcagcatg gcgaaacaaa acgacaaccg ggtgctgctg tgccagtctg gccaatgttt
    17461 tattgtcgat acgtggcaaa ccattgctac tccaggcggc atgagtaccc agtccggcaa
    17521 ttgcagcgag cgccagtaaa atgatgaaat atttttttga cttcaatgat gagcggcaaa
    17581 aagctaacac gtaaactcca cctatagaca agcgcaacca gacaattacc gtgaaattga
    17641 gcgacatttc tggcgataat tagcagttgg tgtaatatta aaaatcctac gatgtcggca
    17701 aaatgcctca aatttttgcc aaatgcaaag cctaaataag aaaaaatata aaaatttcaa
    17761 tatttacgtc taatattagt ttcttaaggt taagttaata ttctatcctt aaaatttcgc
    17821 tccaaatggc aaaatataca caacactctt tatagcaaat ataagtggac aggtattcaa
    17881 tggcggaagg aaaagcaatg tcagaatttt tgcctttttc gcgaccagca atgggcgtgg
    17941 aggaactcgc tgcagttaaa gaggttctcg aatccggttg gatcacaacc ggtccgaaga
    18001 atcaggcgct tgagcaagcc ttttgccagt tgacgggaaa tcagcatgcc atcgcggtca
    18061 gttcagccac cgccggaatg catatcacgc taatggcgtt ggaaattggc aagggcgatg
    18121 aagtgattac gccttccctg acctgggttt caaccctcaa tatgatttcc ttgctgggtg
    18181 caacgccggt aatggtggat gtcgaccgcg atacgctgat ggtcacgcct gaagcgatcg
    18241 aatcagccat tacgccacgc actaaagcca tcattccggt gcattatgcc ggtgcgccag
    18301 cagatattga cgccattcgc gccattggcg aacgttacgg catcgcagtt atcgaagatg
    18361 ctgcccatgc cgtcggtacg tattacaaag ggcgacatat tggcgcaaaa ggtaccgcta
    18421 ttttttcatt tcatgccatt aaaaatatta cctgtgctga aggtggcctg attgtaactg
    18481 ataatgaaaa ccttgcccgc cagctacgga tgctgaaatt tcacggtctg ggtgtcgatg
    18541 cctatgacag acaaacctgg ggccgtgcac cgcaggctga agtcttaaca ccgggctata
    18601 agtacaatct gaccgatatt aacgccgcga ttgccctgac acagttagtc aaattagagc
    18661 acctcaacac ccgtcggcgc gaaattgccc agcaatatca gcaagcactg gcagctctcc
    18721 cctttcagcc attaagcctt cccgcctggc cgcacgttca cgcctggcat ctgtttatta
    18781 ttcgtgtcga tgaacaacgt tgtggtatca gtcgcgatgc gttgatggaa gcgttaaaag
    18841 aaagaggcat tggtaccggg ttacatttcc gcgccgctca cacacaaaaa tattatcgcg
    18901 agcgttttcc cacgctgtcg ttaccgaata ccgaatggaa tagcgaacgc atctgttcgt
    18961 tgccgctgtt cccggatatg actaccgccg atgccgaccg cgtcatcaca gcccttcagc
    19021 aactcgcagg acaataagcc atgtttgaaa tccaccctgt taagaaagtc tcggtggtta
    19081 ttcccgttta taacgagcag gaaagcttac cggaattaat caggcgcacc accacagcct
    19141 gtgaatcgtt ggggaaagag tatgagatcc tgctgattga tgacggcagt agcgataatt
    19201 ccgcgcatat gctggtcgaa gcctcacaag cggagaacag ccatattgtg tctattttga
    19261 ttaaccgcaa ttacgggcaa cattcagcga ttatggcggg attcagtcac gttactggcg
    19321 acttaattat tacccttgat gccgatctcc agaatccgcc agaagaaatc ccccgcctgg
    19381 tggcaaaagc cgatgaaggt tacgacgtgg tagggactgt acgccagaac cgccaggaca
    19441 gctggtttcg taaaacagct tcgaagatga ttaaccggct tattcagcgc accactggca
    19501 aagcgatggg tgactacggt tgtatgctgc gcgcctatcg ccgtcatatt gtcgatgcga
    19561 tgttgcactg ccatgaacgc agcaccttta tcccgattct ggcgaatatc ttcgcccgcc
    19621 gtgcaattga aattccagta catcatgccg agcgtgagtt tggtgaatcc aaatacagtt
    19681 ttatgcgcct gattaatttg atgtacgacc tggtgacctg ccttaccaca acgccgctac
    19741 gtatgctgag tctgctcggc agcattattg cgattggagg ttttagcatt gcggtgctgc
    19801 tggtgatttt acgcctgacc ttcggaccac aatgggcggc agaaggcgtc tttatgctat
    19861 ttgccgtgct gtttactttt attggcgctc agtttatcgg catgggatta ctcggtgaat
    19921 atatcggcag gatctacacc gatgtccgcg cccgcccccg ctattttgtt cagcaagtta
    19981 tccgtccatc cagcaaggaa aatgaataat gaaaaccgtc gtttttgcct accacgatat
    20041 gggatgcctc ggtattgaag ctctgctggc tgccggttac gaaattagcg ccatttttac
    20101 ccataccgat aatcccggtg aaaaagcctt ttatggttcg gtggctcatc tggcggcgga
    20161 aagagacatt ccggtttatg cgccggataa cgttaatcat ccgctgtggg tggaacgcat
    20221 tgcccaactg tcaccagagg tgattttctc tttttattat cgccatctta tttgcgacga
    20281 gatttttcag ctcgctcccg caggtgcatt taatctgcac ggttcgctgt taccaaaata
    20341 tcgtggtcgc gcgccgctga actgggtgct ggtgaacggt gaaacggaaa cgggcgttac
    20401 attgcaccga atggtaaacg tgccgatgct ggggccattg tggcgcaact gcgcgttgcc
    20461 attgcgccag acgatatcgc tattacgctg catcataaat tgtgccatgc cgcgcgccag
    20521 ctactggaac agacattacc cgccattaaa cacggtaata ttctggaaat cgcccagcgc
    20581 gaaaacgaag ccacctgttt tggtcgcaga acgccggatg acagtttcct tgaatggcat
    20641 aaaccggcat ccgtactgca caacatggta cgtgccgttg ccgatccgtg gccgggtgcc
    20701 ttcagctatg ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc
    20761 agcaaagcac agccggggag cgtgatttct gttgcgccac tgctgattgc ctgtggcgat
    20821 ggcgcgctgg aaatcgtcac cggacaggcg ggcgacggca ttactatgca gggctcgcaa
    20881 ttagcgcaga tgctgggcct ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc
    20941 gcccgacgcc gtacccgggt actcatcctc ggggtgaatg gctttattgg caaccatctg
    21001 acagaacgcc tgctgcgcga agatcattat gaagtttacg gtctggatat tggcagcgat
    21061 gcgataagcc gttttctgaa tcatccacat tttcactttg tcgaaggcga tatcagtatt
    21121 cattccgaat ggattgagta tcacgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg
    21181 atagccacgc cgattgaata tacccgcaac ccgctgcgcg tatttgaact cgattttgaa
    21241 gagaatctgc gcattatccg ctactgcgtt aagtaccgta agcgaatcat cttcccgtca
    21301 acttcagaag tttatgggat gtgtagcgat aaatacttcg atgaggacca ttctaattta
    21361 atcgtcggcc cggtgaataa accacgctgg atttattcgg tgtcaaaaca attacttgat
    21421 cgggtgatct gggcctatgg cgaaaaagag ggtttacagt tcaccctctt cctcccgttt
    21481 aactggatgg gaccacgact ggataacctt aatgcagcgc gaattggcag ctcccgcgct
    21541 attacgcaac tcattctcaa tctggtagaa ggttcaccaa ttaagctaat tgatggcgga
    21601 aaacaaaaac gctgctttac tgatatccgg gacggtatcg aggcgttgta ccacattatc
    21661 gaaaatgcgg gaaatcgctg cgacggcgaa attatcaaca ttggcaatcc tgagaacgaa
    21721 gcgagcattg aggaactggg cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc
    21781 catcatttcc caccgtttgc gggctttcgc gttgtcgaaa gtagctgcta ctacggcaaa
    21841 ggatatcagg acgtagagca tcgtaaaccg agcatccgca atgcccaccg ctgcctggac
    21901 tgggagccga aaattgatat gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc
    21961 accgttgatc ttacggataa accatcatga ccaaagtagg cttacgcatt gatgtcgatg
    22021 cctttcgtgg cacccgtgaa ggcgtgccgc gtctgctgga aatcttgagt aagcataata
    22081 ttcaggccag catttttttc agcgtcggcc cggacaatat gggccgccat ctctggcgac
    22141 tggtgaagcc acagtttttg tggaagatgc tgcgctcaaa cgcggcatcg ctttatggct
    22201 gggatatttt actggcaggt acggcctggc caggtaaaga gattggtcat gccaatgccg
    22261 atatcattcg tgaagcggct aaacatcacg aagtcggtct gcacgcctgg gatcaccatg
    22321 cctggcaagc ccgtagcggt aactgggatc ggcaaacaat gatcgacgat attgcacgcg
    22381 gtcttcgcac tctggaagag attatcggtc aaccggtaac ctgttctgcc gctgcgggct
    22441 ggcgtgccga ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg
    22501 attgtcgtgg ggccataccg ttccgtccac tgcttgaatc aggaaaccct ggcactgcgc
    22561 aaattccggt gaccttaccc acctgggatg aagtgattgg tcgggatgtg aaagcagaag
    22621 attttaacgg ttggttactc aaccgcatcc tgcgagataa aggcacgccg gtttatacca
    22681 ttcatgcaga agttgaaggc tgcgcttatc agcataattt tgtggatctc ctcaaacgcg
    22741 cagctcagga aggcgtgaca ttttgccctt taagcgaact gttatcagag acgttgccgc
    22801 tcggacaagt tgttcgcgga aatattgccg gacgtgaagg ctggctgggt tgccaacaaa
    22861 ttgcgggtag tcgctgatga aatcggtacg ttaccttatc ggcatcttcg cgtttattgc
    22921 ctgctattac ctgttaccga tcagcacgcg tctgctctgg caacccgatg aaacgcgtta
    22981 tgcggaaatc agtcgagaaa tgctggcatc cggcgactgg attgtgcccc atctgttagg
    23041 gctacgttat ttcgaaaaac ccattgccgg atactggatt aacagcattg ggcaatggct
    23101 atttggcgcg aataactttg gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc
    23161 cgcgctggtg acctggttta ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc
    23221 cacagtaatt tatctctcat tgtttattgt ctatgccatc ggcacttatg ccgtgctcga
    23281 tccgtttatt gcattctggc tggtggcggg aatgtgcagc ttctggctgg caatgcaggc
    23341 acagacgtgg aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct gcggcatggg
    23401 ggtgatgacc aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt
    23461 agcgacgcaa aaacgctgga aagatctctt tatttacggc tggctggcgg ttatcagttg
    23521 cgtactgacg gttctcccct ggggactggc gatagcgcag cgggagcctg acttctggca
    23581 ctattttttc tgggttgagc atattcaacg ctttgcacta gatgatgccc aacatagagc
    23641 tccgttctgg tactacgtgc cggtcatcat tgccggtagc ctgccgtggc tgggattact
    23701 ccccggtgca ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt
    23761 gagctggacg ataatgccgc tgctgttttt ctccgtcgct aaaggcaaat tgcccaccta
    23821 tattctttct tgctttgcac ctctggcaat gctgttggcg cattacgcgt tgctggcagc
    23881 aaaaaataat cctctggcgc tgcggattaa tggctggatt aacatcgctt ttggcgtcac
    23941 tggcattatt gccacatttg tgatctcccc ctggggacca atgaacacgc cggtgtggca
    24001 aaccttcgag agctataaag tcttttgtgc ctggtcgatt ttttcgctat gggcattttt
    24061 cggctggtac accttaacaa acgtcgaaaa gacctggtct ttcgccgcgc tttgcccgct
    24121 ggggctggcg ttgctggtag gattttcaat tcctgacaga gtcatggaag gaaaacatcc
    24181 gcaatttttt gtcgagatga cacaagaatc acttcagcca agccgctata ttcttactga
    24241 tagcgtcggt gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta
    24301 tcgccagaca ggtgagttga aatacggcct taattatccg gatgcgaaag ggagatttgt
    24361 cagcggtgat gagttcgcaa actggcttaa tcaacatcgt caggagggga ttattacact
    24421 cgtgctttcg gttgaccgcg atgaagatat caacagtctc gccattccgc ccgcagatgc
    24481 catcgttcgt caggagcgtc tggtgctgat tcagtatcgt cccaaatgat ctggctaaca
    24541 ttagtctttg ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc
    24601 tttgtggcga taaacaaacg gcgcaaacat atcgtgctgt ggctgggact ggcgctggct
    24661 tgtcttggtc ttgccatgat gctctggctg ctggtcttgc agaacgtacc ggtaggcatt
    24721 gcctacccga tgttaagtct gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg
    24781 cacgaaccgg tatcgccgcg tcactggtgt ggggtggcgt tcattattgg tggcattgtg
    24841 atcctcggga gtacggtgta atgtgcctga tatggggatt attcagcgtc attattgcct
    24901 cagttgcgca attaagcctg ggttttgcgg caagtcatct gccgccgatg acgcacttat
    24961 gggattttat cgcggctctg ttagcttttg gcctggatgc ccggatactg ttactcgggc
    25021 tgctgggtta tctgctttcc gttttttgtt ggtataaaac gctacataaa cttgcgctaa
    25081 gcaaagccta tgccttgctc agtatgagtt atgtgctggt atggattgct tcaatggtac
    25141 ttcccggctg ggaagggact ttttcgctga aagcactact gggagtagcc tgtattatga
    25201 gcgggttgat gctgattttc ctgcccacga caaaacaacg ttactgagtt ttccctgccg
    25261 ctgtacactg ccgttcccac tcatcggggc tataactgct tgccgagagg actttcaccg
    25321 tatgcagctt ttcacgatta atacaataaa gcgcattctg caaaggcgag agtaaatccc
    25381 ccactttcac ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg ccgccagcat
    25441 cgcagagcat aagtacatgt ctgttatctt gtttattgca gcacgatttt ttgtgccagc
    25501 cattccattg cattatcctg tttgctaaga gttttcacat caatttacag attaacagat
    25561 aaactcttta tcaacggcaa tggaatggca gaaatgagaa gtgaaacgga gggttaatta
    25621 ttgctgacgt tgcacccact cttttagcgc ctgacgtgaa attttaatac cgccgttttt
    25681 cagctccggc ggtaaagtca gccagcgcac cggttgttga aaacgagcca gcttatcttt
    25741 cacccattcg ccaagatcaa cggtttgctg gtcatactcc accaccgcta ccggccgatg
    25801 accaaactcc ttgtcggcaa cggaaacgat aaacacctgc aaaaccgcag gatgtgcagc
    25861 aataacacgc tcgacttctt ccggctgaat accctcaccg ccactgaaga acagattgtc
    25921 taaacgtccg acaatggtca gcttgccatt atgcatctca ccgcgatcgc gcgtagcgta
    25981 ccagccttcg tcattaacca gtgaaaccag ttgcccgtta cgccagtaac cttctgccat
    26041 actggcagcc cgcagccaca cttcattatt aacgattttc acttcccgac ccggcagcgg
    26101 caaaccaacg tctgccaggc cgtcggcttc tttcgcacat accgtggagg caaactcggt
    26161 cagaccatag ccgcaaaagc aacgaatccc ctgctcgcgc gcctgttccg tcaactcgac
    26221 cgggatagcc gcgccgccaa gtaacaccgc tttcagggaa acggaactgc ggttaaccag
    26281 caaacgccag agttgtgttg gcaccagtga agcgtgagta cagcctgcca gcatttgctc
    26341 caatggctgt ttatcacgca ccgtcatccg cgcaccagcg tataaccagc gccacataat
    26401 tccctgaccg gagacgtgaa acagcggtaa agagagcaac cagtcatcgt gatcgccaaa
    26461 cggaatcagc gataacacac cttgcgcact ggcaagatgg gcctgataag tatggacagc
    26521 ggctttcggc aaaccggtag aacccgaggt caacgtcatt gagcacagac gcgtcggctg
    26581 ccacgcagcg gcatgtacgc cttcaaccag ctgaatgtgc agcgacgtta atgccggaaa
    26641 cgtgttttcc ccatccggca ccagcgcaaa ttgcagcgtc agattgggca gcaattcttc
    26701 aagcaacggt tgcggcagct gagggttcac gggcaacacc cgcgccccgc attgcagtaa
    26761 cgccagccag gcgagcagcg tttgcggcgt attccacgcc cgcaacatca cgccgctgcc
    26821 ctcaaccacc ccctgcgccg caaagcccgt agctaactca tcgacgcgag cacaaagctc
    26881 gcgccagttg agttgctcgt cattaagacg taaggcgatg gcttctcccc gcacttgccg
    26941 ccagtgacgc cacggccagt cagagaagat cataacaact gctcctgtac atcagcgtcc
    27001 actaacggca gtgggctccc cggccagcga cgaacctgct gcgcctgcat cagatccagc
    27061 gtgtccagtc ccggaatggt gtctggcgtt aaccaggcag caatccgcgc cagttgcgtt
    27121 agacctaagc tcgactcaat ggaagaactg atcaccgccg tcagccccag cgcatgcgcc
    27181 gcctgtacct gctcgcgtac tttttccaga ctgcccgtga gcgtgggttt gataactacc
    27241 gcgcgcacgc cctcttcagc cacaaaggca aaatccggct cgcgcaggct ttcatcccag
    27301 gcaatggcaa tgccggtttc acgggcaaac gctcgcgaat catcgcgggt tttgcacggc
    27361 tcttcgagaa acgcgatgcg gtggcgataa tccgggttaa catatttggc aaactgctga
    27421 cctttcagcg gtgtccaggc gcgatttgcg tcaagacgca aatgcagatc cggaattgcc
    27481 tccagcaaca gattcaccac catgccgtcg cgcaccgctt cgtacaatcc cactttgacc
    27541 ttcgccactt tctcgcccgg catatcggca agtttgagga tcagatcgtc cggatcgcca
    27601 ttacacagcg gtgccgcacg gtagttggct gcttgcggca acgtatctgt cagttctgcc
    27661 aatgcacagc ttacgccaaa ggccacggaa ggcatctgcg gtagctcgca atcgcctgcc
    27721 agccagttat ttacccaggc aagcagcacg ctttgcgcct cttcccagtt ttcctggctg
    27781 aagcccggca gtggggagat ctccccccac ccttcgcgct cgccctcacg caggcaaacg
    27841 tacagcccgt cgcgggtttt taaccgcctg tcgcgcagaa ccacccccgc gtccatgggg
    27901 atctgccagc ggtatacctg cgcgctacgc attacggatt ccgtttaaat ttgctgaaat
    27961 caggctgacg tttctggttg aaagcgttgc gaccttcctg accttcttcc gtcatgtaga
    28021 acagcatggt ggcgttgccc gccagctcct gcagccccgc ctgcccgtca cagtcggcgt
    28081 tcagcgcagc tttcaggcag cgcagcgcca tcgggctgtt ttgcagcatt tcgcggcacc
    28141 aacggacggt ttctttttcc agatccgcca gcggtaccac ggtgttgaca aggcccatat
    28201 ccagcgcctg ttttgcgtcg tactgacggc acaggaacca gatttctcgc gcttttttct
    28261 gcccgacgat acgagccatg taggaagcgc cccagccgcc gtcgaaggaa ccgactttcg
    28321 ggccagtctg accgaagatg gcattatctg ctgcgatagt caggtcgcac atcatgtgca
    28381 gaacgtgacc accgccgatg gagtagccag ccaccatcgc gacaaccggt ttcggacagg
    28441 tacggatctg acgctggaag tccagcacgt tcaggtgatg tacgccggaa tcatctttat
    28501 agccgccgta atcaccacgc actttctggt caccaccgga gcagaacgct ttatcgcctg
    28561 cgccagtcag aatgatcacg ccgatattgt cgtcataacg cgcatctgcc agtgcctgga
    28621 tcatctcttt taccgtcaga ggacggaagg cattgcgcac ctgcggacga ttaatggtga
    28681 tttttgcgat accgtcggtg gatttttcat aacgaatgtc ctcgaaacct tcggagcagt
    28741 cgtgccattc aaccggtgcg taaggcattg cttcatcagg ataaatcata ttgtgtcctt
    28801 tagtcagaaa cgcaagatct gcgccagact tgcgattacg ccagcgggat tttcccgatg
    28861 cgcgttatgt ccggcgcgag gaatgacatg gcagtcggca gccagttccg ccgccagggc
    28921 gcggaatttg ctgtcacgtt caccacataa ataataaaac gcaaatgtgc gggcgctaag
    28981 gttagcacgt aaatcaggct ggacggcgag agaagtcgcc tccagcatgg cggcaagggt
    29041 tgcgccatta ttgttgctgc gcagcgccac cagctcccgg cgttgatcgt cattgagcga
    29101 ggcaaaaaca ggctgttgat accagtcggc aaataccgcc gttaacggtt ctgtgcgaaa
    29161 acgctgcgcc cattggcgat cggaacgctg acgttccgca cgttgttcag cattttgcag
    29221 ccccggatgc ccgccttcga caataacccc acaaagcccc gccagcccct ggcaagccgc
    29281 catcatcgcc acccgtccgc caagcgagta ccccaccagc cagaagtcaa ggatgttgta
    29341 actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc
    29401 cgccgaacca ccatgaccta ggagatcaac atacaaccgt gagtagtcgg caaacgcctc
    29461 gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg
    29521 taaacctagt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc
    29581 agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg
    29641 gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga
    29701 tgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc
    29761 agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg
    29821 ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg
    29881 ttgagatcgt aaagcgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc
    29941 cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg
    30001 tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg
    30061 ttaccaacaa acaattgccc ctgttcaggc agatagtcgc tgatgcgatg cgccagttgc
    30121 gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc
    30181 gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca
    30241 atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca
    30301 tcaacaatcc agtactcttc tggttcacag cttgcctgcc attgcaggag ccgtttcccc
    30361 gtcaggctac ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg
    30421 gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg
    30481 ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca
    30541 ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt
    30601 ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca
    30661 cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca
    30721 aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta
    30781 gaaaccagcc aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg
    30841 gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc
    30901 tccggcgggc gatcggcggt taagagaatc agtttttctc cggttaaccc ggcttcaatc
    30961 agtgccggat agagatttgc caccgccgtg ccggaggtca caatcaccgc caccggctgc
    31021 ttgctgactt tcgccagccc cagcgccaga tgccccaacc cacgctcatc gaaatgggtg
    31081 tggtgaatga atgcggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcct
    31141 ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc
    31201 cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag
    31261 tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta
    31321 ttccatttgt aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg
    31381 ctcggggtcg gaaccacgga caatgcccgc gccagcatat aatcgcacga cattgccgct
    31441 aatttttgct gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc
    31501 agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag
    31561 atcgcgcggt aagccagcaa ctgctgccgt tggcagcaac tgatgtaaac agatcacatc
    31621 atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg
    31681 cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat
    31741 atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc
    31801 taactgctgc gcctgcttat catcaggatg atttgctact gttcccgcca gcgcttcagt
    31861 acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt
    31921 ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc
    31981 catcatcgcc gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac
    32041 cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca
    32101 gcccgttttg tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg
    32161 cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga
    32221 gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag
    32281 gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa
    32341 gtcagcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg
    32401 ggtaatcgcg cccaggacgg cagcttcttc atcaccatta cgttgttgcc agtagaattg
    32461 cgggtacgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa
    32521 aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag
    32581 tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca
    32641 cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg
    32701 tgccagcaac agtcctagta ccagcccgac ggccgcgccc acaccaattc cttgccaggg
    32761 tttttcgtgg acgtagtcat cagcacgata aacagcctgc ttcgcccgat agtaataact
    32821 gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg catgcgcttt
    32881 cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt
    32941 ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct
    33001 cgttctccat ttgcgtaaaa cctgtcacta actatagaca agagttgttg attacgcctg
    33061 aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac
    33121 agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac
    33181 aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gctgctgccc
    33241 cactttttca ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag
    33301 atcatcatca cttttcagaa tcctcgcata cgccaccagt tcatcatttt tccaaccgag
    33361 aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg
    33421 ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa
    33481 ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt
    33541 atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac
    33601 ttcagccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca
    33661 tccgacccag agcggacttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact
    33721 gcataccgcc tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga
    33781 tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc
    33841 cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag
    33901 cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga
    33961 tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta
    34021 tcgtattgaa gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg
    34081 cgtgccgcct ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga
    34141 tggaaggcag attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc
    34201 tattttcggc gataccggcc cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt
    34261 catggtgcac gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca
    34321 tagctctaca cgccaggctg cgacactagc ccgtgaggct ggagtcggca agctaatcat
    34381 tacccacgtc agctcgcgct atgatgacaa aggttgtcaa cacctgttac gtgaatgcag
    34441 gtcaattttc ccggcgactg aactggcgaa tgatttcacc gtgtttaacg tttaactctg
    34501 cttattatta aaaacagggt gaaacttgcc ctgttatagc aacccgcgct acatatatcc
    34561 acccacacag gcatattgag cgatttttga tatttattca tctccttacg cactatattc
    34621 agagcaataa gttgcctctt ttcatcttga ttcagcacaa cctttgcaac taaaaggagc
    34681 ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaacccaa ctcagtcaga
    34741 catttgcaga aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt
    34801 tatccggaga tgaagaaagt attgagttac tgcataatat tgcgttaggt tatgatgaat
    34861 ttggaaaaaa agctgggtaa tgctgccaac ttactgattt agtg
//
LOCUS       DAJVQR010000035        34789 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVQR010000035 DAJVQR010000000
VERSION     DAJVQR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..2088
                     /locus_tag="OQM52_001813"
     CDS             <1..2088
                     /locus_tag="OQM52_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS1563691.1"
                     /translation="YDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGG
                     NFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFY
                     LNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKT
                     TSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAIS
                     ASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISA
                     TVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRA
                     IVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRY
                     SEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVS
                     DSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQ
                     DLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRE
                     LMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEING
                     SEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKL
                     RGNVQTAQREVAP"
     gene            2085..3173
                     /locus_tag="OQM52_001814"
     CDS             2085..3173
                     /locus_tag="OQM52_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1563692.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3261..4559)
                     /gene="exuT"
                     /locus_tag="OQM52_001815"
     CDS             complement(3261..4559)
                     /gene="exuT"
                     /locus_tag="OQM52_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1563693.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5043..6455
                     /gene="uxaC"
                     /locus_tag="OQM52_001816"
     CDS             5043..6455
                     /gene="uxaC"
                     /locus_tag="OQM52_001816"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1563694.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6470..7957
                     /gene="uxaA"
                     /locus_tag="OQM52_001817"
     CDS             6470..7957
                     /gene="uxaA"
                     /locus_tag="OQM52_001817"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS1563695.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8040..8591
                     /locus_tag="OQM52_001818"
     CDS             8040..8591
                     /locus_tag="OQM52_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1563696.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8596..9840)
                     /gene="sstT"
                     /locus_tag="OQM52_001819"
     CDS             complement(8596..9840)
                     /gene="sstT"
                     /locus_tag="OQM52_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1563697.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10436..11401)
                     /gene="alx"
                     /locus_tag="OQM52_001820"
     CDS             complement(10436..11401)
                     /gene="alx"
                     /locus_tag="OQM52_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1563698.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11672..12670)
                     /locus_tag="OQM52_001821"
     CDS             complement(11672..12670)
                     /locus_tag="OQM52_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1563699.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12749..13441)
                     /locus_tag="OQM52_001822"
     CDS             complement(12749..13441)
                     /locus_tag="OQM52_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1563700.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13518..14021)
                     /locus_tag="OQM52_001823"
     CDS             complement(13518..14021)
                     /locus_tag="OQM52_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1563701.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14106..15242
                     /gene="rlmG"
                     /locus_tag="OQM52_001824"
     CDS             14106..15242
                     /gene="rlmG"
                     /locus_tag="OQM52_001824"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1563702.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15527..15841
                     /gene="higB"
                     /locus_tag="OQM52_001825"
     CDS             15527..15841
                     /gene="higB"
                     /locus_tag="OQM52_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1563703.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15838..16254
                     /gene="higA"
                     /locus_tag="OQM52_001826"
     CDS             15838..16254
                     /gene="higA"
                     /locus_tag="OQM52_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1563704.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16299..18317)
                     /gene="fadH"
                     /locus_tag="OQM52_001827"
     CDS             complement(16299..18317)
                     /gene="fadH"
                     /locus_tag="OQM52_001827"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1563705.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18543..20894)
                     /gene="ygjK"
                     /locus_tag="OQM52_001828"
     CDS             complement(18543..20894)
                     /gene="ygjK"
                     /locus_tag="OQM52_001828"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS1563706.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20911..21330)
                     /locus_tag="OQM52_001829"
     CDS             complement(20911..21330)
                     /locus_tag="OQM52_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563707.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21308..21661)
                     /locus_tag="OQM52_001830"
     CDS             complement(21308..21661)
                     /locus_tag="OQM52_001830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563708.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21662..21982)
                     /locus_tag="OQM52_001831"
     CDS             complement(21662..21982)
                     /locus_tag="OQM52_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563709.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22116..23549)
                     /locus_tag="OQM52_001832"
     CDS             complement(22116..23549)
                     /locus_tag="OQM52_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1563710.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23612..24061)
                     /locus_tag="OQM52_001833"
     CDS             complement(23612..24061)
                     /locus_tag="OQM52_001833"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1563711.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24058..27144)
                     /gene="ebgA"
                     /locus_tag="OQM52_001834"
     CDS             complement(24058..27144)
                     /gene="ebgA"
                     /locus_tag="OQM52_001834"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1563712.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27327..28310)
                     /gene="ebgR"
                     /locus_tag="OQM52_001835"
     CDS             complement(27327..28310)
                     /gene="ebgR"
                     /locus_tag="OQM52_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1563713.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28528..28859
                     /locus_tag="OQM52_001836"
                     /pseudo
     CDS             28528..28859
                     /locus_tag="OQM52_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28901..30280)
                     /gene="ygjG"
                     /locus_tag="OQM52_001837"
     CDS             complement(28901..30280)
                     /gene="ygjG"
                     /locus_tag="OQM52_001837"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1563714.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30698..32218
                     /gene="aer"
                     /locus_tag="OQM52_001838"
                     /pseudo
     CDS             30698..32218
                     /gene="aer"
                     /locus_tag="OQM52_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32372..32995)
                     /gene="nfeR"
                     /locus_tag="OQM52_001839"
                     /gene_synonym="yqjI"
     CDS             complement(32372..32995)
                     /gene="nfeR"
                     /locus_tag="OQM52_001839"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1563715.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33272..34017
                     /gene="nfeF"
                     /locus_tag="OQM52_001840"
                     /pseudo
     CDS             33272..34017
                     /gene="nfeF"
                     /locus_tag="OQM52_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34071..34146)
                     /locus_tag="OQM52_001841"
     tRNA            complement(34071..34146)
                     /locus_tag="OQM52_001841"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34111..34113),aa:Ile,seq:cat)
     gene            34271..34777
                     /gene="mug"
                     /locus_tag="OQM52_001842"
                     /pseudo
     CDS             34271..34777
                     /gene="mug"
                     /locus_tag="OQM52_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8485 a         8947 c         9142 g         8215 t
ORIGIN      
        1 tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta ttatcgttat
       61 gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac actatttaat
      121 gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga tgggatgcgt
      181 atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac cccagtaatg
      241 gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact tttgggatcg
      301 ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc aggcagctac
      361 agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct tgtgccgttt
      421 accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc aggtaaaact
      481 acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg cctgccatta
      541 catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag tgctttcgag
      601 ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat cagcgccagc
      661 gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg gagtaatccg
      721 ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg tacaaccgac
      781 agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac agtttcactg
      841 aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa cactgatgat
      901 agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca gacaacagcg
      961 caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat gcgtgactgg
     1021 attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga taacgacgac
     1081 aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag caataacaac
     1141 agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg cgatcaaacg
     1201 tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga acttggcgta
     1261 accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg ttgggatggg
     1321 caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa tcatgatcat
     1381 ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta tggcgttaat
     1441 ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt gaaaggcttc
     1501 tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc aggcagccga
     1561 actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt tcagtctggt
     1621 tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa catcattaac
     1681 ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg cgaagtgtct
     1741 gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt gaaaaaattc
     1801 ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg atttacactt
     1861 gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt ccttaagtgt
     1921 ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt tacttgtagt
     1981 gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt gaaacagcct
     2041 aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga aatcgattga
     2101 ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct cctgacgtga
     2161 ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc tggaaggatg
     2221 aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct gacctccaac
     2281 aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact gaacaacgca
     2341 gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta agtagcgatg
     2401 aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag gattctaata
     2461 gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag tttcttacgc
     2521 tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg aaggggaaag
     2581 ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc ctgaatacgc
     2641 tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg ccaagggtgc
     2701 agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa gatctgacca
     2761 tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat gaaatcatgc
     2821 tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct atctatcgtc
     2881 agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa atgtacaacc
     2941 cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg aacagcatta
     3001 acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg atgtgtgtgc
     3061 cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag gccgggtact
     3121 acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat taaccctgca
     3181 aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg cggcttcgtc
     3241 gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt ttcctgcgcc
     3301 acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc cagcaggtca
     3361 aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag tgcaccgaca
     3421 accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc gttcgccgtc
     3481 gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc agacagtgcc
     3541 tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt ggtgaacaga
     3601 ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat cttacgggaa
     3661 acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc accgaggata
     3721 caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc tttcaggtta
     3781 aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc ccaggccggt
     3841 tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag gatctgacca
     3901 acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt aataatatag
     3961 tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa aatcagccat
     4021 gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg ccagctgtgc
     4081 atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg
     4141 ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc
     4201 agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagctagg
     4261 ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac
     4321 attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg
     4381 accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt
     4441 aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc
     4501 acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg
     4561 taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg
     4621 aagatgttag ttacgaccgg gtaaccgaaa aaaaaaccgg cacgccccga acgttgccat
     4681 gaaagtttcg tgctaattcg gcttccgtac cggttaaccc gattcatcta acaaatgaat
     4741 gaatcggcaa tttgcattcc gtcgacttat gatttgcgac ggcagaaaga taacttgtca
     4801 tacaacctta aaaggtgaga gccatcacaa atgtgcgaat atttgtaggg acattacctg
     4861 acgacagcga ggccagtact ggcgcggcct gcagcgagat ttaccacttt gagagtaatt
     4921 tttttaacta cgtttattga tctaactcac gaaaatatct tcggactctg gaaattggtg
     4981 tgataacttt gtcagcatcg caccataagc aagctagctc actcgttgag aggaagacga
     5041 aaatgactcc gtttatgact gaagatttcc tgttagatac cgaatttgcc cgccgtctgt
     5101 atcacgacta cgcaaaagac cagccgattt tcgattacca ttgccatttg ccgccgcagc
     5161 agattgcgga agactatcgt tttaaaaacc tgtatgacat ctggttgaag ggcgatcact
     5221 acaaatggcg cgctatgcgt accaacggtg tggctgagcg tctgtgtact ggtgatgcgt
     5281 ctgaccgtga aaaatttgac gcctgggcgg cgactgttcc gcacactatc ggcaacccgt
     5341 tataccactg gacgcacctc gaactgcgtc gtccgttcgg tatcactggc aaattgcttt
     5401 ctccgtcaac tgccgatgaa atctggaacg aatgtaacga actgctggcg caggataact
     5461 tctccgcgcg cggcatcatg cagcagatga acgtgaaaat ggtcggcacc accgatgacc
     5521 cgatcgattc tctggagcat cacgcagaga tcgccaaaga cggctctttc accatcaaag
     5581 tgctgccgag ctggcgtccg gacaaagcct tcaatatcga acaggcgacc tttaacgact
     5641 acatggcgaa gctgggcgaa gtttccgata ccgacattcg ccgctttgct gacctgcaaa
     5701 ctgccttgac caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg tctgaccatg
     5761 cgctggacgt ggtgatgttt gctgaagcga acgaagcgga actggacagc attctggcgc
     5821 gccgtctggc tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa actgcggtac
     5881 tggtgttcct tggtgccgaa tatgcacgtc gcggctgggt acagcagtac cacattggcg
     5941 cgctgcgtaa taacaacctg cgtcagttca aactgctggg gccggatgta ggctttgact
     6001 ccatcaacga ccgtccgatg gcggaagagt tgtctaagct gctgagcaag cagaacgaag
     6061 aaaacctgct gccgaaaacc attctctact gcctgaaccc gcgcgataac gaagtgctgg
     6121 gcaccatgat cggtaacttc cagggcgaag gtatgccggg caaaatgcag ttcggttccg
     6181 gctggtggtt taacgatcag aaagacggta tggaacgtca gatgacccaa ctggcgcagc
     6241 tcggtctgct gagccgcttt gtcggtatgc tgactgacag ccgtagcttc ctgtcataca
     6301 cccgtcacga atacttccgc cgcattctgt gccagatgat cggtcgctgg gtggaagcgg
     6361 gcgaagcacc ggcggacatc aacctgctgg gcgagatggt gaaaaatatt tgctttaaca
     6421 atgcgcgtga ctacttcgcc attgaactga actaaggtct ggggttgata tgcaatacat
     6481 caagatccat gcgctggata acgtcgcggt cgctttagca gatttggctg aaggcacaga
     6541 agtcagtgtc gataaccaga ctgttacgct gcgccaggat gttgctcgtg gacataaatt
     6601 tgcgttaacg gatatcgcaa aaggggccaa cgtcatcaaa tatggcctgc cgattggtta
     6661 tgcattggcg gatattgcgg cgggggaaca cgtccacgcc cacaatacgc gcacgaatct
     6721 gagcgatctg gatcagtatc gctatcaact tgattttcag gatctgcctg cgcaagcggc
     6781 agatcctgaa gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc gtaacgagct
     6841 gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg cggcaaatcc agaaccgttt
     6901 cctgaaagag accaacaacg ccgaaggtac cgacggcgtg ttcctcttca gccacaccta
     6961 tggctgctca caactgggcg acgatcacat caacacccgc accatgctgc aaaacatggt
     7021 gcgccacccg aacgcgggcg cagtgctggt gattggtctg ggctgtgaaa acaaccaggt
     7081 tgccgcattc cgtgaaacgt tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg
     7141 ccaacagcag gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc tgtataacgt
     7201 gatgcgcaac gataaacgcg agccaggcaa actcagcgaa ctgaagtttg gtctggagtg
     7261 cggtggttct gacggtcttt ctggtattac tgctaacccg atgctggggc gtttctctga
     7321 ctacgtgatt gctaacggcg gtactaccgt actgaccgaa gtgccggaga tgtttggcgc
     7381 agagcagttg ctgatggacc attgccgcga cgaagcaacg tttgaaaaac tggtcaccat
     7441 ggtcaacgac ttcaaacagt actttattgc ccatgatcag ccgatctacg aaaacccatc
     7501 accggggaac aaagcgggcg gtatcaccac gctggaagac aaatcacttg gctgtaccca
     7561 gaaagcgggt tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc
     7621 agggctgaac ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg
     7681 tgcgggctgc catatggtgc tgttcagtac tggtcgcggc acgccatacg gtggatttgt
     7741 gccgacggtg aaaatcgcca ccaacagtga actggcggcg aagaaaaaac actggatcga
     7801 ctttgacgcg ggtcagctga tccacggtaa agcgatgccg cagttgctgg aagaatttat
     7861 cgacaccatc gttgagtttg ccaacggtaa gcaaacctgc aacgagcgta acgacttccg
     7921 cgaactggcg atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt tgttaataaa
     7981 aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca tacctgctaa ggatttatca
     8041 tgaccgcgta ttggctggcc cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa
     8101 catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat agcgccgtta
     8161 ttggcgtaca tttttttctt ctgggcacct atcccgctgg tgccagtgcc atccttaatg
     8221 ccattcgtac attgattacc ttacgcacgc gcagcttatg ggtaatggcg atttttattg
     8281 tgctgactgg cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtta
     8341 tcggtacgat tgtcagtacc tgggcgctgt tccgctgtaa agggctcacc atgcgctgcg
     8401 tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg tcgataggcg
     8461 gcacgatgat tgagggtagt tttctgctta tgaatggcct gaatatcatt cgtttctggc
     8521 ggatgcagaa aaggggaatt gatccgttta aagtagagaa aacccctccc gccatagacg
     8581 aaaggggtta aacaattaat tacgcagggc gctatttgcc agacgatcgt cttccgcctg
     8641 gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc
     8701 ctgcaatacg ccgataataa agccgacggc aaccacctgc atggcgatat cgttcgaaat
     8761 accgaacata ttacaggcca gtgggatcag cagcaaagaa ccccccgcca cgccggatgc
     8821 gccacaggca cacagagaag ccactacgct caacagcagc gctgtgggca gatcgaccgg
     8881 aatacccagc gtattaaccg cagccagcgt caacacggta atggtgattg ctgcgcccgc
     8941 catattgatg gtggctccca gcggaataga aactgaatag gtatcgcgat ccagattcag
     9001 cttttcacac agcgccatat tcaccggaat gttcgctgca gagctgcggg tgaagaaggc
     9061 atacacaccg ctttcgcgca ggcacagcag caccagcggg aacgggttac gacgaatttt
     9121 ccaccacacc agcaatgggt taaccaccag cgccaccagt aacatacagc caaccagcac
     9181 gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca gggtagaaga
     9241 aaccagccca aaaataccga acggtgcgaa gcgaatgacc agtttcacca taaaggtaac
     9301 ggcattcgac atatcattga ccaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa
     9361 gccgaggccg atcgcccaca ccagaatccc gatgtagtta cctttcagca gggcgtcgat
     9421 ggggttggaa accatgctca ttaccagtcc gcgcatcact tcgacaatgc ctgacggcgg
     9481 cgaaatatca cccgcgctac tggacaagtg cagggtagaa gggaaggcaa agctgaagac
     9541 tactgcggcc agagctgcag agaaggtgcc cagcagatag aggaacaaaa taggacggat
     9601 attggttttc tgcccgtgct ggtggttagc aatagatgcc attaccagca tcaacaccag
     9661 gatgggggca acagctttca gtgcgccgac gaacaaagta cctaacagac caacagcttc
     9721 cgccgcgggt tttgagatcc atgccagaag aatccccaga acaaggccga ccaggatttg
     9781 ttttaccagg ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat
     9841 ttttcgatcc tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga cgcatctttc
     9901 aggatgtaac aaagtgtttg caaggtcgag tataaggaaa gttacagctt cgggaagcga
     9961 aaaatgctgg attttaaaga gaggtcatgt tttgcaactt aatggttgca tttatttaac
    10021 ataaacagag ggaaggactg gctactaagg ctgattagat aacatatggg caaaactatt
    10081 tggaatatga gaacggttgc gctcctttaa cgtgttatgc catataaagt cactggcgca
    10141 gggagcgcgc agggggcggc caatcgccgc cgccccctgc acccccaggc tctggcgaac
    10201 aaaatcgccg ctgcgcggtt ccctcagcct atcccttgcg gctaccgggt cgggcgcaag
    10261 gtaacgtccc tgtaagatgc gccctcgccc cggcctccag ggaacgcttc ggcgattttg
    10321 acgccaccaa cacccgtgcg gtctttttgt aaagagctat aagccgatta cataattaat
    10381 agctcacctt tgtctggaat gagttatctc taggaatggc ttaatataca gcgaatcatt
    10441 ctccccgctg cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca
    10501 gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca
    10561 gcatcttgat accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac
    10621 gctctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatt gcaaacaggt
    10681 ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa gatagccgga atgctatcca
    10741 cggcaaaaat cacgtcgctc aattccacca gaatcagtac cagcatcagc ggtgtggcgt
    10801 acagcaatcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca
    10861 aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg
    10921 cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt
    10981 cgaactgcga aatcagccag ctgcctgtga agatcatgat ggtacgcaga acaatcgccc
    11041 cgagcacgcc atacaccagc acgcgacgtt gtaatgccgc cggaacggag aaatagctga
    11101 acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacccg
    11161 tgagaaaggc cagggcctgt ggatcggcaa cggcgcgacc ttcggtttgc accagatacc
    11221 accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag
    11281 cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca
    11341 gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca
    11401 tagaagttcc ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt
    11461 atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg
    11521 tattcctttc gggttgcccg gtgaccggat gtggtttttc actcatcgta atgacgatcg
    11581 accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca ttcagtcagt
    11641 caatggtctg ttacatacct tttacactgt tttactttgg caagtatagt tttacgctat
    11701 ctgccggaaa aatcacccca gtttggcggc ggatctcggt cagcagtttg gctgtgatgc
    11761 gactgaccgc cagccctgga tgatccacca gatgctcatc caccagggta gcgaacagct
    11821 ctgcttcgta gagcatggta ttaatatgct gcggctgggt gagatcctgc atctggctgc
    11881 cacgcggcac aaagcagact ttctggcatt cagacagttt ttcaatcacc agcgatcccg
    11941 cttcgccctg aatctcgctt cccaggacag aatcactgac ctttgagtgc tgcaaggtga
    12001 cgctgaaatc accgtaatcc atcaccacca cgccgtgggc gtcaacgccg cttgccagca
    12061 aactcgccgt tgcctgcacg cttttcggct caccaaataa cgccaccgcc gacgccaggc
    12121 agtaaaagcc gatatccata attgaaccgt tagagaatgc cggattaaag gtgttgggat
    12181 tctcaccgtc caggtaacgt tgataccgcg aggaatactg gcaatagttg aaaaagactt
    12241 tacgcagttt gcctgctttc ggcagcgcct ggcgcaacaa atgaaagttc ggcaggcagg
    12301 cggttttaaa tgcctcaaac agcaccacct gattttcgcg cgcacaggca atggcggcat
    12361 ccacttccgc cagattcgac gccagcggtt tttcgcaaat cacatgaatt ttatggctaa
    12421 ggaaaagttg tgtctgggaa aagtgcaggg aattcgggct ggcaatatac accgcgtcaa
    12481 tggcatcgct ttccgccatc gcttccagcg acgtaaacag atgctcgaca gaaaaatcat
    12541 tggcgaagtg ctgagcctgc tcaaggctgc gggaatatac ggcggttaac ttgtatttac
    12601 cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga
    12661 aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt
    12721 tttcatcttc gacgtgcgca gatacgcatt attctttcgc cggacagcca tgctcactaa
    12781 acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg atttttgcgt
    12841 caatcaccgc gctgacacga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca
    12901 tattgaggtc tggcgcgtta aacccgatag cgtcgatgcc gtactgtttt gccagccaga
    12961 tggtgcgttg gttatgaaat tcttgcgaga taatggtgat atggttttcc ccaaacacct
    13021 ttttggcacg cactaccgaa tccagcgttg agaatccggc atagtcgcaa aagatcactt
    13081 tcgccggtac gcctttagcg atcaaagcct gctgcatacc tgatgcttca tcgtaatttt
    13141 tacgtccgtt atcgccactc accagtagcc atttcacttt tccggcgtgg tacaacgctg
    13201 ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctcgccccc agcaataaac
    13261 ccacgttacg cgccgggacg gcgttgacat cgccccaggt cagctgcttg ctggcgttga
    13321 ccataacgcg atcggcgata aaaatggtcg ctcctgccac aagtaacagt aagcaggcaa
    13381 tctttagagt ccggcgagaa aaacaaatac ggagaagaag gcgggcaaat gcgcgcagca
    13441 tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaaga aagaaaagat
    13501 aacgcctgac atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc
    13561 cgggtgtcga actcaagctg gtggtactgc ggctccatat ggcagcacaa ttgatagaac
    13621 gctttgttat gctctttctc cttcaggtgc gccagctcgt gcaccacgat catgcgcaaa
    13681 aacggttccg gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt tgctttcagc
    13741 ttgctgccct gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg
    13801 atcttattgt catacatcac tttattgatc ggcggggcat tacgcagaaa ctgatttttc
    13861 agatcctgag tatattgcca gagagcttta tcggtggcgt agtcgtgtgt tcccggatag
    13921 cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac ctgcgaaagc
    13981 agctgctcgg gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag
    14041 aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag
    14101 ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga
    14161 cggatgacgt taacccgcta caggcgtggg aagcggcaga tgaatatttg ctgcaacagt
    14221 tggacgacac agaaatcctc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa
    14281 gctgcgcgct ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg
    14341 cgacgcgcga gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag tttctcgaca
    14401 gcaccgccga ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg
    14461 cattgctgga acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta
    14521 ttgccggtgc caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc
    14581 tcggcccgac caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca
    14641 atgaaccgcc gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact
    14701 ggactatcca taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct
    14761 tctttatgca acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta
    14821 atggcgttat tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg
    14881 atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg
    14941 cgttggatcg cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct
    15001 ttaatgctgt gctctgcaac cctccgtttc accaacaaca tgcgctgacc gataacgtcg
    15061 cctgggagat gttccactac gcccgccgct gcctgaaaat caacggcgag ctgtatatcg
    15121 ttgccaaccg ccatctggat tacttccata aactgaagaa aattttcggc aactgcacca
    15181 ctattgcgac gaataataaa tttgtggtgc tgaaagcggt gaagctggga cgtcgtcggt
    15241 aagattggga gttacctgcc ggatgcggcg taaacgcctt atccggccta cgttcagcac
    15301 gttaccccca ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgctcagttc
    15361 tggaatcgcg cttccataaa acaaaaaatc cctgtcacca tcctctacat tctcttgttt
    15421 agcgtttttc tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctgtc
    15481 aacggatgtt atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac
    15541 tcaaaaagca ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc
    15601 tctggggaac acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt
    15661 cccatctctg gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa
    15721 tgaattacgt gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt
    15781 tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg
    15841 attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc accttttctg
    15901 gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg
    15961 ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa
    16021 gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata
    16081 gccgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt
    16141 ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac
    16201 gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac
    16261 gtgccgaatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt
    16321 gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt
    16381 aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc
    16441 cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca
    16501 tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc
    16561 gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg
    16621 ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc
    16681 gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg
    16741 gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca
    16801 atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca
    16861 tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac acgcggcacg
    16921 atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga
    16981 ttgagtttca gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg cagcgtttcg
    17041 taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg
    17101 tgcgcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc
    17161 ccagcaggtc cagcgccgac caccgccaga ttttttttct gcacggcggg aaggattggc
    17221 attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag
    17281 atctgatcga ggcagccctg gttgcagcca atacaagtgt tgatctcatc ggctcgccct
    17341 gattgcgctt ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat cgatactata
    17401 tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg
    17461 gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg
    17521 cgcggcacgg gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat tccggtgttg
    17581 ataatggtcg cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc ggcaaaagtg
    17641 ccgccgcctt cgactaggtc gagcatcgac aaccggtaaa taataatgaa gtcgttgccg
    17701 acgcgttcgc gcaccgtacg cactacttct acggcaaatc gcatccggtt gcggtaatcg
    17761 ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac
    17821 ccttcagagc ccatcacttc gacgccatcg tatccggcct cccgcgccag ttgcgcgcag
    17881 cgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa
    17941 cggttgatgg gggcctgcaa tgcggacggt gcgaccagat gcggttggta gctgtagcgc
    18001 ccggtatgca aaatttgcag ggcgattttg ccgccttcct gatgtacggc ttcggtaatg
    18061 gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg
    18121 cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt
    18181 tcggcataaa acgcagccag ccgttcagca ccgtccgggt attcctccag cccggtgtgc
    18241 attgaaccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac
    18301 agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt agtcggatga
    18361 gttctaattt agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga
    18421 ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc
    18481 ttgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agccgttgca
    18541 aattactgct tacggaaaaa atcgttatac aacatataca aatgagcggc actccaggag
    18601 aaatttggtg cgccttgctg cgcgcctgtc agcgggttgt aattctcctg aattgggcca
    18661 tcggcggtta atcctttggc gtgcctgaag aacgtatccg ccagcttcag ggcatcatca
    18721 cgataaccgt aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc
    18781 ccgcgccagt agatatcagc gccaaaagcc ggattggtta acgccgccgt tcccagcgga
    18841 acaaatgtgt tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcattggcc
    18901 tgcgttgccg caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca
    18961 atcggtttgc ccgcgcagcc gttcgccagt ggtttatctt caatacgtac gtcatagtag
    19021 aactgggtag tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac
    19081 tggcgatagc gtttggcctc ttctggctta ccgaggatgg ttgccatctc cgccagataa
    19141 tggttatcgc tgtacatata gctggcctga tccaccgact cctgcaatag cgagtagccc
    19201 agcaacgttc cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg
    19261 ccgccattag cgacatattt atccaactgt tctttgtcga taaaaccaaa gacggcggca
    19321 tcatcacgcc ccgattccca cgacgcagcg acctgtgccg gaatttccag actgtcgtac
    19381 tggcctttct ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg
    19441 ccttttttca ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt gtcgcgggtc
    19501 gcgccatatt ccggcacccc gttgccgtta tgatcgcggt tacgtaacca ccagtcgtga
    19561 taggccacca gtttcgggta catctctgcc aaccaggctt tatcctgggt gacgttgtat
    19621 acttccatca ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg
    19681 ccatcgccac cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca
    19741 tcctgcggac gcacgctgtc gccaggctgg atctgccagg agaagaccgc gcggatattc
    19801 tctttggcga tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac
    19861 ggccaggtct gattgccgga gaaccagcgt ccggtcaccg acggcgtgac ggtgttataa
    19921 ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg
    19981 acgcgcgtct gttccggcgt cgcatccgga ttggttaacc ctttcttcag gtattcttcc
    20041 cagcgttgct gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc
    20101 atttgctctt tgctaacttc ctgagcggtc agcagatggg aataggtggt gtagagcgtg
    20161 gtcgaaccgt tgatatgcgc cttactggta aagcgattac cgttgatttc agtctgcacc
    20221 ggcagggatt tatgcacctg atattccgat tcgccggagg tcagcagatc ccaggtggcg
    20281 cgcactttgc caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctgatag
    20341 tcagggtatt cgccagcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt
    20401 ttttccagca gttcgccatc ccacaccaga tccagcggtt tattgctgat gattttagtt
    20461 tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg cacatctttt
    20521 gctgtcagtt tttgcaccag cgcgccggga atactgtatg cctccagcgt gaagttgact
    20581 ttcttgccgt cttgccagac agtcaggcgg tcgaaattgc tggccataaa gttgatgtat
    20641 tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc gtctggcaac
    20701 agatgaccat gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa
    20761 tcgtaatcct tcatgtactg cggcgcacca gtacggttaa tcacgttttt ataattgtcg
    20821 gcgttagcgg catgagcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata
    20881 gttttgattt tcatgagatt acagtcctta ttaccagtag aaatattgca tcaggaagat
    20941 ttggtcgctg ggatcgttag aggcattttt catgtagaag cggctatcaa aagccgtggc
    21001 gaaacgtccg ccgccatatt cgtaccagat accaaactgc atgaaagagg tggtttcatc
    21061 gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg gtggagagat aaacgccttt
    21121 taacgcttca tacatggcgc tcaccatcat cccgctttca cttcccggta cgtccagccc
    21181 gtcggcacgt ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa acaggcccgt
    21241 gagatagagt tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg tgatgccttc
    21301 ggcaaggtca tactggatgt aaccgttcat ccattgccgg ataggtgtat ttgtcgtcgt
    21361 agcgatcgta cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt ccggcaggtg
    21421 tggcggtgac gctataacga aagttgccgc ttaagttctg taccttcagc gcggtcataa
    21481 tgtcgcgggt attgggaatg acgtagccca aatcgggtgt gaagtcgccc caccattgca
    21541 ggtcatccag cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca tcattggtac
    21601 gataaccgcc atagaaacgg ttaatcccac cttcgaaacc accccagcta tggtcaggca
    21661 cctaggcatt accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa atcaggccga
    21721 accaggagcc gtacttcatc tccaggccac cttcaatata ggtgccgtcg ctgtatttat
    21781 gtttattctc accctctaaa tccatatatc caccaagccc taattcgccg taaaaacgaa
    21841 atgaaggaga agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt tctgccggac
    21901 gtgcttcttc tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt aatgctctgc
    21961 atggtgcagt gattaatttc attgtgttaa tccatgagct aaagaaaaag tacagccttt
    22021 tatcgttgta attataaaaa tataaaggca gaaactattt tccctctcct catccaggaa
    22081 gagggaaaat gcctgctcat tttttattag tctgcttaat caacattgct ggctggcgtc
    22141 gcttcaatat ggcgctcttc agccgttaat ccctttatat atttgctgta tttccaccac
    22201 gcaaagccga ggaagataac aataccgccg acgttataaa aaatgatggt cagaatattc
    22261 gcgccagtcg ggaatgtcga agcgacaaac cctacggcaa atatcgcaat cagcattgaa
    22321 acaacgataa taccggtgcg gcgggagccc atacggaaat cgcgtggcag gtgatctaat
    22381 ttggcgcgta aattcaggta agccagcatg atgaataacg gcggaagcat ggacgctgcg
    22441 gcggtcatat taataatggt attcatcaga tcctgcacgg tattggaacc gagcatcggg
    22501 ataatcatca gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc cggaacgccg
    22561 ttttcgttaa gttcgacggt tttcttacca aagatgcctt ccgggatttc ggagaagaaa
    22621 attttcaccg gcgttgcggt ccacatcagc agggaaccga acattgcggt aaaggacacc
    22681 agaccgacaa agcgattcat caacgcttcc ggcagaccaa aatacgccgc catgccgtgg
    22741 aatacctgca ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa gacgttaatc
    22801 agcaccgagg agacggaata cagtacgccg ataaaaatcc cggcgaggat gatcacttta
    22861 acgaacgatt tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga ctccgcgcca
    22921 cctgcggcca taaagatcca ggtggtaacg ccgaggaacg cccagttgaa gttcgggatc
    22981 atcgcgtcaa cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc cgtacctgcc
    23041 agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga ggtaattggc
    23101 cccagcattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag caccatactg
    23161 ataacggtgg tggcaaccgg cgtcataata tattcgtagc cgaggaaggc atacgaagca
    23221 taagcaataa cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa ccagtaagtg
    23281 taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccacgc gtagacaccg
    23341 gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa gggaataaaa
    23401 taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc aatattatta
    23461 ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa gccgaatttg
    23521 ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgtgt agggtagcgg
    23581 tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa ccatcttcga
    23641 tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga tacgcttcat
    23701 ggatatcgca aataacgatt tgcccttcgt ggacctctac ggtttctccg cagcctttta
    23761 aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc tctttcggcg
    23821 catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag cgtcgatgcc
    23881 cggtaaatag cgcatcggtc gcggagtcat tttccacacg gtaggtcaat gaatcaccga
    23941 tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca acgcagcgtt
    24001 gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg atgatcctca
    24061 ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact catacgacgc
    24121 caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc cgtagctgaa
    24181 gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt tggagccgag
    24241 gccaagtagc tggtggtcga ggttcagggt gatgtcatca ctgcgctgca gctcgttaca
    24301 gtgctgggca gcgtggatgt tttcctgggt atagtgccag gcgctgaagt taattgggcg
    24361 ctgcggaacc accagcagac cgttgccgtg gcggttagtc agtgccgtcc agcggacatg
    24421 ctgacggttg ccgttgttct gcgggaaggg atagttctcg aacatggcat cgacggtgct
    24481 gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc ccggtccacg
    24541 accgtaatac gccacctgat cgtattcgcc gttaatcccc atggtgaaac cgatgcacgg
    24601 aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa cctggccatc
    24661 ggcagcgatg cgccagatgt aggtacagcg catcccgaag tcaaacaccg gcggtgcaat
    24721 aactgtgcgg ctgatgatca acacttcacc atcgctctgt tctacggcaa agtcgcgcag
    24781 atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact cctgcttgtg
    24841 gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca gcgactcgcc
    24901 atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg cgaagttgta
    24961 gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg cattatttgg
    25021 tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag tggcgataga
    25081 atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt tgaggaacgc
    25141 ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt tcggcgcaac
    25201 gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca cctctgcgtg
    25261 cagggtgtag tcatcaagcg tggtaaacca cagtttattt tcgactttca actcgccgcg
    25321 agtcagcgcg tggattttta ccggcgcgat aacctgtttg tactctttca ggcccgatcc
    25381 cggcgtctga tcggaataga tcaaaccatc aagacagaag ttatagttgt tgggatagtc
    25441 gccgtagtcg ccgccgaatt tataccagac attgccgtgg tcgtcctgtg cctggatccc
    25501 gtggtcgcac cactcccaga cataatgacc ctgaatgcaa tcgtgcttat agaagacgtt
    25561 ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca tgagcatatt cacagatgat
    25621 gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc agcggcacgc gggtgtacat
    25681 ggtggaaata atatcgacca cttcagcatc gcgatcttct tcgtaatgaa ccagtcgcgt
    25741 gtcatccagc gctttcgccg catggtacat cgcgcggatg ttacagccat agccggattc
    25801 attgcccagc gaccagatga tgatcgacgg atggtttttc tgtgcgtgga tatggcgaac
    25861 aatgcgctcg acgtagactt tttcccactg cggatcgtcg gtaatacggc tgatatcgcc
    25921 gacattagca aagccgtgcg attcgacgtc ggtttccgcc atcacaaaca ggccgtagat
    25981 atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga gcggtacgca cggagttgat
    26041 attgtgctgc ttcatcaact ggagatcttt ctcgacgcga tccattccaa cggcgcggcc
    26101 tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc atcacataac ggttattgat
    26161 ccagaacaga ccgtcgcgca ctttgatatc acggaagcca acgcgttgtg gcaccacttc
    26221 cagaacgttg ccgttggcgt ctttcagcgt catgaccaga tggtaaagat aaggggattc
    26281 tgctgaccat tgctgcggct gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc
    26341 aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt tcgccatcaa acagggtata
    26401 ttccagcgtc gtgacgacag gggaggcggc gagattttcc agcaccactt cgcaggaaag
    26461 cgtggcatcg caataggctt cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt
    26521 taggtgtttt ccgaccagat aaacatcgcg gaagatcccc gccgaccacc acatatcctg
    26581 gtcttccacg taggtagagt ccgcccactg catcacgcgc acacacaaca ggttgtcgcc
    26641 ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc
    26701 cacatactga ccgttaacgt agacttcaaa ataggtttcg acgccgtcaa atttaatcag
    26761 cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata cgttgatagg caccggttgg
    26821 gttatcgctg gggacaaacg gcacatcgat ggggaacgga aaaccttcgt cggtatattg
    26881 cagtttgccg tgaccttcca tttgccacat ggcggggacg gtaatatgcc cccagtcagc
    26941 cattaactca gaggtgaagg cttctggtac ttgcagcgga tggtcaaaaa agtggaaatt
    27001 ccactgactg cttaagggca gaaacaggct gctggtttcg cgggcaaagg tacgcgcttg
    27061 cgcaacagaa tcatatgaaa aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag
    27121 ctgaatgttt tcccagcgat tcatagggcc tcccggtaga gagaaatcgg aaccattcat
    27181 ggtgatggta gtttgtgtaa ttgctggggg tttagtaaaa tttttactaa aaagcagcag
    27241 gtaaatgcat ttactttaac tggatcacga aaataaacgg cagcactgtc cggcaaaaat
    27301 gattgccgaa cagtgtgtaa ggggatttaa cgggtcgtgc cgcgcagttt taatttgctg
    27361 ggaacgaaga ctagcagcgg cagcgcgcga ccatcgcggg ctttttcata caccaggtta
    27421 acgccctgac ttcccatcat ctcggaatgg atgcgcacgg tggagagcgg cggaaaggta
    27481 aatcgcgcgg tggggatatc gttaacgctg ataagcgaaa tatcttgtgg gatgttcagg
    27541 ccacgttcat gaattgcccg cagtacgccg atagcaatgg aatcggaagc aacaaacagt
    27601 gccttcggat agtcttcccg cgccagcatt tgttttgcca gttcataacc cgacgaactg
    27661 gaaaaaccgc cgcgccagat atcctcttcg cgtaccactt gtttcagtcg gccatattcc
    27721 gcaaaggcga cctcacgaat atccgccttg ccaggctcat cttcaccgcc aataaaacca
    27781 atacgattaa cgccctggtt gatatagaag tcgatgattt ctttactgat gcgtgccaga
    27841 tcgatatcca ccgcatcgta accgctgccg ggttcgtgaa agtcgataaa acagatattg
    27901 tcggtcaacg cgctggcagc ggcgcgcagg gcgggcgtgg gtttgccgac aattaaaata
    27961 ccggtgacgt ttttaatgtc tggtaatccg ttgtgttcat aacagttggt cagttcgatc
    28021 gccagctttt cgcactgagt ttcaatgccg tggcggatcg ccagatagta aggatcgttg
    28081 atctccagct cctgctggta gctgtagata gccagaatat ggtgttggtt gactgcgcct
    28141 gtctgtagtt tacgggcgct actggtcttg tactccagct tttcggcgat ctcgagaatg
    28201 cgatgtttcg tctcttcttt cacattcaat gtcggatcgt catttaagac cctggatact
    28261 gtcgccaggg atacgccagc ttcgattgcg atgtctttta atgttgccat gtttaccttt
    28321 tttactttcc ctgatgtgcg cccccattgt agaggaagtg cttatcgggt cacctgtttt
    28381 cagtaaataa agcgcgtttc tttgacgctg taacggtaac acagcggctt aggaatgtga
    28441 tcacgcctgc attctccatt tttagcctac aggattgaga aaaacttttc tatagtttag
    28501 taaaatttta ctcgaaggga ggaagcaatg gaaaccgtgg cttacgctga ttttgcacgt
    28561 ctggaaatgc gcgtcggaaa gattgtggaa gtgaaacgcc atgaaaacgc cgacaagctg
    28621 tacatcgtac aggttgatgt ggggaaaaaa cactgcaaac cgtgaccagc ctggtgccgt
    28681 actacagcga agaggagctg atggggaaaa cggtggtggt cttatgcaat ctgcaaaaag
    28741 cgaaaatgcg tggtgaaacg tcggaatgca tgctgttgtg tgcggaaacc gatgacggca
    28801 gtgaaagcgt gttgttaacg ccggagcgga tgatgcctgc gggcgtacgc gtcgtataaa
    28861 aagatcggat ggcgacgtcg tatcgccatc cgatttgata ttacgcttct tcgacactta
    28921 ctcgcatggc cgccagcgcc ttacgcgccg ctttgatcac cagttcacac tgttcaatgg
    28981 tcagtgtcag tggcggttca atgcggatcg ttttggcgtt attgagcgtt ccggccacca
    29041 gtacgcgctg gcggaacatc tcgctggcaa agttatagcc gatttcgtta tcaacaaact
    29101 caatcgccat caacatccct ttaccacgcg cttcctgtac cagatcggga tattcccgcg
    29161 ccagttgacg gaaaccgtcc agcaacatat cgcctttttg ctcagcctga gccggtaagt
    29221 tctgctccag caacacattg atggtcgcca gcgccgccgc acaggccagc gggttgccgc
    29281 caaaggtggt ggtatgcagg aatgggttgt cgaacagaac tgaaaacacc tcttcagtgg
    29341 cgatggtcgc gccaatcggc atcacgccgc cgccgagggc ttttgccaga cacagaatgt
    29401 ccggctgaac gttctcatgt tcgcaggcaa acatcttgcc cgtgcgcccc atgcccgttt
    29461 gtacttcatc gaggatcatc agtgcgccga actcatcgca cagcttacgc acggcggtga
    29521 gatagcccgg cggcggcaga attacgccac cttcgccctg aatcggttcg aggatcaccg
    29581 cagccacatc atcaccggtt tttttgcact cattaagagc cgtgcgcatg gcttcgatat
    29641 tgccaaacgg cacatgacgg aaccctggca gcagtggcat aaacggtttg cggaaggtcg
    29701 atttcgccgt ggccgacagc gcgccaagtg atttaccgtg gaacacgccg ctggtggcaa
    29761 taaaagtaaa tttgccgcgc ggcgactgat aagccttcgc cagcttcagc gccgcttcaa
    29821 cggactcggt gccgctatta cagaagaagc tgtatttcag tttaccgggc gttagcgcag
    29881 caagggtttt cgccagcata gcccgtaacg gatcgagtag ctcctggctg tgcagtggtt
    29941 gtttcgcaag ttgattctgt acggcggaaa ccacaactgg attacggtgc cccacgttga
    30001 aaattccaaa acctcccagg cagtcgataa actcctgtcc ctgggtgtcg acaagcgtat
    30061 ttaaacctcc cgcttgccac tctacggctc cgtaatcccc gccggcggta acagatttgc
    30121 gatactctaa aaaccccgga ttgacatgct ctttgaagta ttcaatcacc tctcggttaa
    30181 gtgctttcat ctcctcatga tccagcgttc gcttctcaat gagattcagg gcgtgggcgc
    30241 tgcacgctaa agccgatgcg ctcgaaggta acctgttcaa aatatgctcc ggaggctcgc
    30301 gtatcacatg ataccgattt aagtattgca gggattgcgc cactccggct gcgatcgcga
    30361 aaatcgggat aaacacaaat gcggttatca tttgtgcaat atttaatgct atccgttaac
    30421 atttgttgtt attttgcacg gtttttgtcg ggtatggatt tgatgattgc tgcgcaaatg
    30481 cacgttttca gggcaataat tgcctgattt ttgggcaaga taattaacgt tttcgtatag
    30541 taacagagtg aaagtatttt aaatcaaaga attagatttg tgctttaatc gcaaattgcg
    30601 atctaaatca aattaatcgg ttaaagataa ccgcagcggg gccgacataa actctgacaa
    30661 gaagttaaca accatataac ctgcacagga cgcgaacatg tcttctcatc cgtatgtcac
    30721 ccagcaaaat accccgctgg cggacgatac cactctgatg tccactaccg atctgcaaag
    30781 ctatatcact catgctaatg acacttttgt gcaggtgagc ggctttacct tgcaagagtt
    30841 acaagggcag ccgcacaaca tggtgcgtca cccggatatg ccaaaagcgg cgtttgcgga
    30901 tatgtggttc accctgaaaa aaggggagcc ctggagcggc atcgtgaaaa atcgccgcaa
    30961 aaatggcgac cattattggg tgcgggccaa tgcggtaccg atggtgcgcg agggaaaaat
    31021 cagtggctat atgtcgattc gtacccgggc gacggatgaa gagattgcgg cggtggagcc
    31081 gctgtacaaa gcgttgaact ccggacgtac cagtaagcgt attcataaag gcctggtggt
    31141 gcgtaaaggc tggctgggta aactgccttc attaccgctt cgctggcgga cgcgtggagt
    31201 gatgaccctg atgtttatct tgctggcggc catgctttgg tttgttgctg ccccggtggt
    31261 gacgtatttc ctctgtgtgt tagtggtatt gttggcaagc gcttgttttg aatggcagat
    31321 tgtgcgcccg atagaaaatg tcgcccgtca ggcactgaag gtggcgaccg gagagcgtaa
    31381 tagtgttgag cacctgaatc gcagcgatga gctggggctg acattacgcg cggtagggca
    31441 gcttggcctg atgtgccgtt ggctaattaa cgatgtctca agccaggtgt ccagtgtcag
    31501 aaatggcagt gagacgctgg cgaaaggcac cgatgaactg aacgaacata cccagcagac
    31561 agttgataac gttcagcaaa cggtggcgac catgaaccaa atggcggcgt cggtgaaaca
    31621 gaactctgcc acggcgtcgg ctgccgataa actttctatc accgccagta atgcggcagt
    31681 gcagggtggg gaggcgatga ccacggtgat caagacaatg gacgatatcg ccgacagtac
    31741 ctagcgcatt ggcaccatta cttcgctgat taacgatatt gcgtttcaga ccaatattct
    31801 ggccctgaat gcggcggtgg aagcggcgcg tgccggcgaa cagggcaaag gttttgcagt
    31861 ggtggcaggg gaagtgcgtc atttagccag ccgcagcgct aatgctgcca acgatattcg
    31921 caagctgatt gatgccagtg ctgataaggt gcaatccggt tcgcagcagg tacacgccgc
    31981 cggacggacg atggaagata ttgtggcaca ggtgaaaaac gtcacccagt tgatcgccca
    32041 gattagccat tcaacgctgg aacaggccga tgggctttcc agcctgaccc gtgcagtgga
    32101 tgagcttaac ctgatcaccc agaaaaatgc cgagctggtg gaagagagtg cgcaggtgtc
    32161 ggcgatggtg aaacaccgcg ccagccgact ggaagacgcg gtgacggtgc tgcattaatc
    32221 ggtatgccgg atccggcggt tggagcacaa tgcctgatgc gatgctggcg catcttatca
    32281 ggcctgcggg gtgtgtagcg ggtgtaggcc ggataaggcg cgtcaagcgt cgcatccggc
    32341 aattgcaccg cgccactggc ggatgcggcg attaatccag ttgcgtaata tcaaaagcgg
    32401 cgcggtcgat caccgcaatg atctttttta tttgtgcatc actgatatcg ctctggttga
    32461 cgcgtaaatc cagcaccgct ttaaaattat ccagcgcccg cttcatttgc gggttctggc
    32521 gcagcgccgc gccaacgcaa cgcgctttga tgcgttcttc aatcatctcc acttgttcgc
    32581 ggttttcttc cagccactgc gcgccttgtt cggtcagcgc aatctgcttc ttaccacctt
    32641 cctcttcgcg gatggtaatc agcgactgct cctgcagaaa atccagcgtc gggtagatga
    32701 cgcccgggct tggggtgtaa ttcccctggg ttagattctc aatcgcttta atcaattcgt
    32761 aaccgtggct gtcatcgcgc gagagaatat ccagaatcac cagacgcaat tcaccgtgac
    32821 caaagaaacg ttgccgacga ccgccgccgc gaccgtggcg accaccgcaa cattgaccgt
    32881 gttcatgctg gtgaccgtgt ccgcagtgct cgtgttctga cttctcacct ttgcagcagc
    32941 cctcatggcg tggctggcct tcatgtttac aacacccttc gtgatgatgg ctcatatcag
    33001 cctccttttg cttgattaga tatatctaat ttatatctga tgtgagcatt actgcaagta
    33061 caaaaatgat ttttaaacgt ggaagcacct cacatctagg ccggataaga cgttcacgcc
    33121 gcatccggca gcattgctat ggcactgaat gccttcacat acttcagaca tatcagttct
    33181 acaaatcgct tgcatttatc atgattaaca atcattatca tttgcgaatt ttatttagat
    33241 atatctgatt accatcacga aggcgataac aatgaataac accccccgct acccgcagcg
    33301 cgttcgcaat gatctgcgct tccgtgaact gactgtgcta cgcgttgagc gcatcagcgc
    33361 cggttttcag cgcattgtcc ttggcggcga ggcgttggac ggttttacat cgcgcggctt
    33421 tgacgatcac agcaaactct tctttcctca acctgacgct cactttgtgc cgccaacggt
    33481 aacggaagag agcatcgtct ggccggaagg accacgccca ccgtcgcgtg actatacgcc
    33541 actgtatgac gaactacgcc atgaactggc gatcgatttc tttattcacg acggtggggt
    33601 cgccagcggc tgggcgataa acttacggtg gcaggtccgc gcggttcgct ggtggtgccg
    33661 gaagattacg cgtatcagct gtatgtctgc gatgaatccg gaatgcccgc actgcgccgc
    33721 cgcctggaaa cgttgagcaa acttgccgtt aaaccgcaag ttagtgcgct ggttagcgtg
    33781 cgggataacg cctgtcagga ttatctcgcg caccttgatg gttttaatat cgaatggctg
    33841 gcacatgatg agcaggcggt agatgcgcgt ctggcgcaga tgcaaatccc tgccgatgat
    33901 tacttcatct ggataaccgg cgaaggtaaa gtcgttaaga atttaagccg ccgctttgaa
    33961 gcggaacagt atgacccaca gcgggtacgt gcagcggctt actggcacgc aaagtaactg
    34021 atatataaaa agaaaaaggc tgacgatttc tcgtcagcct ttgctatatc tggtggcccc
    34081 tgctggactt gaaccagcga ccaagcgatt atgagtcgcc tgctctaacc actgagctaa
    34141 ggggccatgg tagcggatta taaagtaact ccgtgccgca atccagccat taccgcgcgc
    34201 ctgctgtttt tataaacaat gtattttcaa tccgttatac ttttcctggt gatgttagaa
    34261 aggagtaaat atggttgagg atattttggc tccagggtta cgggtcgtgt tttgcggtat
    34321 caaccctggg ctttcatccg ccgggactgg ttttcccttt gctcatccgg caaatcgctt
    34381 ctggaaggtg atatatcagg ccgggtttac cgaccgtcag ttgaagccgc aggaggcaca
    34441 gcatctgctg gattatcgtt gtggcatcac caaactggta gaccgtccaa cggtgcaagc
    34501 caatgaagtt tcaaagcagg agctgcacgc aggtgggcgt aagctgattg aaaaaattga
    34561 agattatcag ccgcaggcgt tggctattct gggcaaacaa gcatatgaac agggattcag
    34621 ctagcgcggt gcacagtggg ggaaacaaac gctcaccatt ggttcgacgc agatttgggt
    34681 gctgccaaat cccagcggtt taagtcgcgt ttcactggag aaactggttg aagcgtatcg
    34741 cgagctggac caggcgctgg tagtgcgtgg gcgataaaaa acgtcaccg
//
LOCUS       DAJVQR010000036        34445 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVQR010000036 DAJVQR010000000
VERSION     DAJVQR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..332)
                     /locus_tag="OQM52_001843"
     CDS             complement(<1..332)
                     /locus_tag="OQM52_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563716.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OQM52_001844"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OQM52_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1563717.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OQM52_001845"
     CDS             complement(1693..2073)
                     /locus_tag="OQM52_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCS1563718.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OQM52_001846"
     CDS             complement(2087..2503)
                     /locus_tag="OQM52_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563719.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OQM52_001847"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OQM52_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1563720.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OQM52_001848"
     CDS             complement(3504..3971)
                     /locus_tag="OQM52_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1563721.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OQM52_001849"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OQM52_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1563722.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OQM52_001850"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OQM52_001850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1563723.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OQM52_001851"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OQM52_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1563724.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OQM52_001852"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OQM52_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1563725.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OQM52_001853"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OQM52_001853"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1563726.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OQM52_001854"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OQM52_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1563727.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OQM52_001855"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OQM52_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1563728.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OQM52_001856"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OQM52_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1563729.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OQM52_001857"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OQM52_001857"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1563730.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OQM52_001858"
     CDS             complement(14626..15441)
                     /locus_tag="OQM52_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1563731.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OQM52_001859"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OQM52_001859"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1563732.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OQM52_001860"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OQM52_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1563733.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OQM52_001861"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OQM52_001861"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1563734.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OQM52_001862"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OQM52_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1563735.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OQM52_001863"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OQM52_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1563736.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OQM52_001864"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OQM52_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1563737.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OQM52_001865"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OQM52_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1563738.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OQM52_001866"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OQM52_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1563739.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OQM52_001867"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OQM52_001867"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1563740.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OQM52_001868"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OQM52_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1563741.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OQM52_001869"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OQM52_001869"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1563742.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OQM52_001870"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OQM52_001870"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1563743.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OQM52_001871"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OQM52_001871"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1563744.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OQM52_001872"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OQM52_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1563745.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OQM52_001873"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OQM52_001873"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1563746.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OQM52_001874"
     CDS             31575..32189
                     /locus_tag="OQM52_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1563747.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OQM52_001875"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OQM52_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1563748.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OQM52_001876"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OQM52_001876"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1563749.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8697 c         9302 g         8338 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gtcacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgtc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcatcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 atttcctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ctctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgta acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgataatt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgtat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtaa tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgcag ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ttgtagcggg aaagcgtatt atgaacaccc
    34441 cgcgc
//
LOCUS       DAJVQR010000037        33879 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVQR010000037 DAJVQR010000000
VERSION     DAJVQR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..248)
                     /locus_tag="OQM52_001877"
     CDS             complement(<1..248)
                     /locus_tag="OQM52_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1563750.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            864..1001
                     /locus_tag="OQM52_001878"
     CDS             864..1001
                     /locus_tag="OQM52_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563751.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1601..2380
                     /gene="ipaJ"
                     /locus_tag="OQM52_001879"
     CDS             1601..2380
                     /gene="ipaJ"
                     /locus_tag="OQM52_001879"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCS1563752.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3327..4256)
                     /gene="virB"
                     /locus_tag="OQM52_001880"
     CDS             complement(3327..4256)
                     /gene="virB"
                     /locus_tag="OQM52_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCS1563753.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4473..4709)
                     /locus_tag="OQM52_001881"
     CDS             complement(4473..4709)
                     /locus_tag="OQM52_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1563754.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4713..6614)
                     /gene="ipaA"
                     /locus_tag="OQM52_001882"
     CDS             complement(4713..6614)
                     /gene="ipaA"
                     /locus_tag="OQM52_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCS1563755.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6623..7621)
                     /gene="ipaD"
                     /locus_tag="OQM52_001883"
     CDS             complement(6623..7621)
                     /gene="ipaD"
                     /locus_tag="OQM52_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCS1563756.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7672..8763)
                     /gene="ipaC"
                     /locus_tag="OQM52_001884"
     CDS             complement(7672..8763)
                     /gene="ipaC"
                     /locus_tag="OQM52_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCS1563757.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8783..10525)
                     /gene="ipaB"
                     /locus_tag="OQM52_001885"
     CDS             complement(8783..10525)
                     /gene="ipaB"
                     /locus_tag="OQM52_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCS1563758.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10531..10998)
                     /gene="ipgC"
                     /locus_tag="OQM52_001886"
     CDS             complement(10531..10998)
                     /gene="ipgC"
                     /locus_tag="OQM52_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCS1563759.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11055..11681)
                     /gene="ipgB1"
                     /locus_tag="OQM52_001887"
     CDS             complement(11055..11681)
                     /gene="ipgB1"
                     /locus_tag="OQM52_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCS1563760.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11697..12086)
                     /gene="ipgA"
                     /locus_tag="OQM52_001888"
     CDS             complement(11697..12086)
                     /gene="ipgA"
                     /locus_tag="OQM52_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCS1563761.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12099..13583)
                     /gene="icsB"
                     /locus_tag="OQM52_001889"
     CDS             complement(12099..13583)
                     /gene="icsB"
                     /locus_tag="OQM52_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCS1563762.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13897..15513
                     /locus_tag="OQM52_001890"
     CDS             13897..15513
                     /locus_tag="OQM52_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCS1563763.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15523..15885
                     /gene="ipgE"
                     /locus_tag="OQM52_001891"
     CDS             15523..15885
                     /gene="ipgE"
                     /locus_tag="OQM52_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCS1563764.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15885..16343
                     /gene="ipgF"
                     /locus_tag="OQM52_001892"
     CDS             15885..16343
                     /gene="ipgF"
                     /locus_tag="OQM52_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCS1563765.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16352..17467
                     /gene="mxiG"
                     /locus_tag="OQM52_001893"
     CDS             16352..17467
                     /gene="mxiG"
                     /locus_tag="OQM52_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCS1563766.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSNEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17475..17726
                     /gene="mxiH"
                     /locus_tag="OQM52_001894"
     CDS             17475..17726
                     /gene="mxiH"
                     /locus_tag="OQM52_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCS1563767.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17739..18032
                     /gene="mxiI"
                     /locus_tag="OQM52_001895"
     CDS             17739..18032
                     /gene="mxiI"
                     /locus_tag="OQM52_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCS1563768.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18038..18763
                     /gene="mxiJ"
                     /locus_tag="OQM52_001896"
     CDS             18038..18763
                     /gene="mxiJ"
                     /locus_tag="OQM52_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCS1563769.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18760..19287
                     /locus_tag="OQM52_001897"
     CDS             18760..19287
                     /locus_tag="OQM52_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCS1563770.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19229..19924
                     /gene="mxiN"
                     /locus_tag="OQM52_001898"
     CDS             19229..19924
                     /gene="mxiN"
                     /locus_tag="OQM52_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCS1563771.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19917..20324
                     /gene="mxiM"
                     /locus_tag="OQM52_001899"
     CDS             19917..20324
                     /gene="mxiM"
                     /locus_tag="OQM52_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCS1563772.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20308..20736
                     /gene="mxiM"
                     /locus_tag="OQM52_001900"
     CDS             20308..20736
                     /gene="mxiM"
                     /locus_tag="OQM52_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCS1563773.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20866..21498
                     /gene="mxiE"
                     /locus_tag="OQM52_001901"
     CDS             20866..21498
                     /gene="mxiE"
                     /locus_tag="OQM52_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCS1563774.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21485..23185
                     /gene="mxiD"
                     /locus_tag="OQM52_001902"
     CDS             21485..23185
                     /gene="mxiD"
                     /locus_tag="OQM52_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCS1563775.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23204..24271
                     /gene="mxiC"
                     /locus_tag="OQM52_001903"
     CDS             23204..24271
                     /gene="mxiC"
                     /locus_tag="OQM52_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCS1563776.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24284..26344
                     /gene="mxiA"
                     /locus_tag="OQM52_001904"
                     /gene_synonym="virH"
     CDS             24284..26344
                     /gene="mxiA"
                     /locus_tag="OQM52_001904"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCS1563777.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26357..26758
                     /gene="spa15"
                     /locus_tag="OQM52_001905"
                     /gene_synonym="spaK"
     CDS             26357..26758
                     /gene="spa15"
                     /locus_tag="OQM52_001905"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCS1563778.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26762..28054
                     /gene="spaL"
                     /locus_tag="OQM52_001906"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26762..28054
                     /gene="spaL"
                     /locus_tag="OQM52_001906"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCS1563779.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            28147..28485
                     /gene="spa13"
                     /locus_tag="OQM52_001907"
                     /gene_synonym="spaM"
     CDS             28147..28485
                     /gene="spa13"
                     /locus_tag="OQM52_001907"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCS1563780.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28472..29350
                     /gene="spa32"
                     /locus_tag="OQM52_001908"
     CDS             28472..29350
                     /gene="spa32"
                     /locus_tag="OQM52_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCS1563781.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29344..30225
                     /gene="spa33"
                     /locus_tag="OQM52_001909"
                     /gene_synonym="spaO"
     CDS             29344..30225
                     /gene="spa33"
                     /locus_tag="OQM52_001909"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCS1563782.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30230..30880
                     /gene="spa24"
                     /locus_tag="OQM52_001910"
                     /gene_synonym="spaP"
     CDS             30230..30880
                     /gene="spa24"
                     /locus_tag="OQM52_001910"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCS1563783.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30895..31155
                     /gene="spaQ"
                     /locus_tag="OQM52_001911"
     CDS             30895..31155
                     /gene="spaQ"
                     /locus_tag="OQM52_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCS1563784.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31155..31925
                     /gene="spa29"
                     /locus_tag="OQM52_001912"
                     /gene_synonym="spaR"
     CDS             31155..31925
                     /gene="spa29"
                     /locus_tag="OQM52_001912"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCS1563785.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31932..32960
                     /gene="spa40"
                     /locus_tag="OQM52_001913"
                     /gene_synonym="spaS"
     CDS             31932..32960
                     /gene="spa40"
                     /locus_tag="OQM52_001913"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCS1563786.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32973..33215
                     /locus_tag="OQM52_001914"
     CDS             32973..33215
                     /locus_tag="OQM52_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563787.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10545 a         4951 c         6803 g        11580 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgtccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacacttgtt gactacctgt gcggcatctg
      361 gtatcaaggt ggcgtgaagc cggttcaggt tctcatgcgg aacgttggaa cctatatccc
      421 cccgatacca aaggcgaaat gtaagtgaca aaatgcaatg aacacgttct ccgttaactg
      481 aggagcatgg tgataaataa aggtaaatgc agaaggtgta gagtctcgca gatttctgac
      541 atcatagagc ctaacattag atgcataacg ctacagattg tagaatctct acaggtgaaa
      601 tatacactgg ttggcgttta tgtcgcaaaa catgtcattc aggttcatct caccaataag
      661 gatatgagtg aagtggagga taagtgagtc tgctgtcaga gttttctggt gtatgtcagt
      721 aactgcgaac catgtctgct tgatatggga gcttatggag gctctctgta caaggttagg
      781 gagctaacaa agcagggata aaaagtccgc ctgatgcagg cacggttcgt taaatctttt
      841 gccattggta acaaaatggt gcgatgaatg ttcaggctat ctttatcttg atggtggttc
      901 agctgtttgg taaagaaatc gctgtaacaa tagaagaact gcaggcagta ctggtcccac
      961 atcgtttgct cagtcaactg gtgaagttcc gtagcgcgta aattaatgct ttgcatgaaa
     1021 cgttactgga gtggatgaaa gaatacctgc atatatcatt actgcgttgg attaccagta
     1081 caacagattg aatgaattgg acatgttgat agacggcatt gaaaagcagc ttataagcat
     1141 agtgcggcag agttaattgg agagcacttc gccttctgag tattaagtaa gcatggtggt
     1201 acctgcggat actatctatt cactgtgctc ggtcggcagc cttactgacc aaggagttgg
     1261 gggagttgat aacaaagttg aaaaaacagc tctcaaccaa ggtggttatt gtaacaatag
     1321 caaataagct ggtgtgacct gtatcacaat aaccgattat ggacgtaaat acgacaaaaa
     1381 tatgtcagta tcaggcggta ttaattgctg atgctgatgg acattaactc ttaataaaag
     1441 ggtggatgct gaaaggttgt tatggtgacg gacgtgatga cgacggaaaa gagtatgagg
     1501 ttttggattt aaagttcgtt cggggaataa ggacccggtc agctaattct catagtagtt
     1561 cgcattgctg gatgcaatac tgtgctgcaa ttactcgtcg atgtcggaac aacggaagcc
     1621 ttgcaaacgg ggatgtattc atacaggtgt tatgttatat ggggttttat tgcagggggc
     1681 tataccaaga gaatatatga tatctcatca gactgatgtg agggtaaatg agaatagagt
     1741 aaacgaacaa ggttgttttt tggcgaggaa gcagatgtat gacaattctt gtggagctgc
     1801 cagtttactc tgtgctgcaa aggaattagg ggttgataaa ataccacaat acaaagggtc
     1861 tatgtccgaa atgacaagga agagttctct tgatttggat aatcgttgtg agcgggattt
     1921 atacttaata actagtggta attataatcc gagaatacac aaagataata tcgcggatgc
     1981 cggttattct atgcctgata agatagtcat ggctacgagg cttcttggat tgaatgctta
     2041 tgtcgttgaa gaaagtaaca ttttttcgca agtaattagt tttatttatc ccgatgcaag
     2101 ggacttatta attggcatgg gatgtaatat agtacatcaa cgagatgtgt tatcttctaa
     2161 ccaaagagtt ttggaagctg ttgctgtatc ctttattgga gttcctgtcg gcctccactg
     2221 ggtgttgtgt agacctgatg gaagctatat ggatccagct gtaggagaaa attattcttg
     2281 tttttcaact atggaattag gtgcaagaag aagtaattct aactttatag gatatactaa
     2341 aataggtatt tccatagtta taactaatga ggctttgtaa attgtcgttt tttatctgtg
     2401 aaggagcttg aatattttgg ggtggggggc ataacaaatg atattactga gtacaaattt
     2461 agctatattg ggcggattag ttgtgtggga agacgaaatg tttctcttga aataagtgaa
     2521 ctccctcaag gaatgattgt gtgcttaaat atttaagtgc tggtaagttt gagaaggctg
     2581 acatattttg cagagcaaac agtgattgac ttggatgata tttttatttt agtcactcgt
     2641 tgtaattgga gtatgcttta ttttatttga aaatagatgt attttatacc cgcaatttaa
     2701 actgtaagcc ctatgagggg tatggatatt ataagttact ctttgaccaa gcataacttt
     2761 tggggttaga aaaataaatc tcttgttaat aattaatatg attttaaaat caaacttgtt
     2821 tataattata aaataatgta agtcggtcat tttatgggaa gattaggaga ataaaacagt
     2881 aattatattt gagaaatctg ctaatatatt cttaaagctt ttttgatact gtgaacgaag
     2941 tctgttaatg gtggattttt tgtttatcac catgtaaaaa aaattgagta gtaaaagcgt
     3001 tgactgctaa tcctcgctgt ttttattgaa acgattaaaa atacgctgaa tattttcggc
     3061 cagtcactca tattcacata aaattttgac catcatgccg catcccttag ttgcacaatt
     3121 cattttcaat gatatgaata ttactaattt caccagataa ttttctcgtg gtcagcaggg
     3181 tatgagtttt caccgtaatt ttaaggaaac acagtgcgtg gggattgaat aacagatagc
     3241 cgacggaatt accccaccgg ctgaaagtca agttcaacga agatttttat tatctgaatt
     3301 gggcagttta catcagtgtt cgatgtttat gaagacgata gatggcgaga aattatatcc
     3361 cgaatagctt catctatttc tctctgaact gttttgttta ttttagataa tgtaaatatt
     3421 agagttctgt ttgttttatt ctcttttctt tttatgtaag tccgtttgtc tttagaaata
     3481 aataacgagt caacttttag cgaagggttt tgctttctgt ttttgctttt ctttatgatg
     3541 tttaaaattt cttttttata tatgtccggg ggcaaatttg tatcaaggtc ttttatttct
     3601 tcctttaata ttggtagtgt agaactaaga gattcattag ccttttcaag tcctttataa
     3661 taattgaata atatcttgta gtcattaaag ttaagttctg acgcgattgg aaatagagat
     3721 attatttcct gtggaacgct tgctgcctga aaggcacgag tgactttcgc gcgagacaga
     3781 ttctcttttt tggctatgtc tttataggac atccctgata ctttcagaaa attaagacca
     3841 ataccaagtt ctcggatgct atgctctttt gctgtttgta tatcgtttgc tagttttctg
     3901 gcatcaagag tagatatata ttcttttgaa tatagaactt caagatctgc tcctgcatat
     3961 attgcagatg ctcttctacg agtgccatcc agaatttcaa ttctaccatc aatctccctt
     4021 cctattacag ggaagaattg ttgtagcttt atagttttta tgatatcggc taatgattct
     4081 tctgttaggg attcttgatc gcgtacattc gttttttggt tgacgaaggt taaatcttgg
     4141 atccttttat gaggaatctt ggctttgata aaggaaactt tattaccaga atggagtgta
     4201 aactctttct tttggccttc ctttatactt aacaagtcgt tgcacaaatc caccatatca
     4261 caccctgttt attcatattg atttatattg acaaaagtta aatgcagtgt gagcaagatt
     4321 gtatgtaact gttgatgata gtcaacggta gtcttaaaaa ttactatttt aatagctgga
     4381 ttgatgcaat ttatctttat tggataaaag aataatattc ttgtggagct ctgatgtgag
     4441 agctccatag atgaaaaaat gcttatcaat tactatattg attttgtact atttttgact
     4501 acagaaataa gatctccaat agtaataatg tgttctaatg tagattcatc tatacgtaga
     4561 ttgaactcaa aactaatcat atccagcatt tcaatcagag aaagagaatc ggcagaaaga
     4621 tcttcaataa atttagtttc ttcagatagt ttgctgtacg ctataccata gcaaaaggcc
     4681 actattgata atattttttc ttttatcata ttttaatcct tattgatatt ctttaatact
     4741 tttgataggg aagtggttac atcttttgct gctttatata tattattgtt ttcagctgta
     4801 atatcattgt tattttttaa atcagatata tcatctgtaa gtaattctgc agaggtatcg
     4861 tcaatttttg acaatacctt gctgagggcg ctagatactt cttttgcctt ttcatagatc
     4921 gcatgatttt tatcaattgt atctgcgttg gtttctgtta cgtagtttct tatatttgca
     4981 tctgtaactt ttaatttgtt gtttattcta tgttctgttg tggattttgt aatgtccttt
     5041 gatttttcct gtaactctcg ttcttgtgtt cctgatttag ttccaatgag agatataaga
     5101 ttacttaggg agtttgttac tttttttgaa gcttcaaata tcgtttcccg tgttaaatat
     5161 gtgtctccat ttcttgataa aaaatcagtc tgaagtactt ttgaatcagt gtttgagttg
     5221 aatttattga tttgcgaagc tactctatga gaaagagaag cgctactttt agttgtatct
     5281 gatgagttat cattactgct tgtcactcct tccgggtgga gagggggggt ggttgaagag
     5341 ttctgtatgt tcagaggcct gtgtccccga gaaagagagt tatttgcaga tctttcttga
     5401 ctactcgtag ttaacaggtc tgaacctatt ccatccgaat tatctggagt atggttgaaa
     5461 gccttttcta aagctgaatt atttgcaaat gtctggacag tatctgatat agagtgtggt
     5521 agaacggaag ttactttttg aactagtgct ctttgtggat ttagtaatga gtttccgtta
     5581 cgggataata gatcgtttgt ctgagaagta ggagactgtg catcattttc tgggttctcg
     5641 tgatagctat tgtcatatac tctgttgtca aaaactctat tatcgtaagt tctattatca
     5701 ttatttatat gatagtgaat tacaggttgg gatgttttct cattggctcc gggggttggt
     5761 ccgccatctg gcatatcttc gggaggtgta gggaggccat cttggatagg atgcgaatct
     5821 tttatatctt ctaaaatatc attaaggact tttctcaaca gctcaggaaa cacggctact
     5881 gtatttaagt ctcttgacag tttacctata ccaaatcctt tactatcaat agtattttta
     5941 cgttgattgc gcaaactatc aaaggcaagt tttgcctgtg cgtcaactaa atcatctagt
     6001 ttgagagatg aaacagtttc attacttaac tttaattcag caagaatatt attcttgata
     6061 aatgatgata cttcagtatt gaggagtttt tgtcccaatg ataaagcttc accagatgtg
     6121 tgttgttgtg ctaatagtgc aagtctgtgc tcaatgtttt gtcgtaattg atggagtttc
     6181 ttattctttg ccgaccatgg tccaaaggca gaatcttggg ataatttatc ttttataatt
     6241 agttccgaat actgtgcgac aattttttga agtccctttt cgttttcaaa attaagacca
     6301 ttttgatgta ctaaggagta caaaacattt gaaaattcga gaatctcgct tttggcatac
     6361 gcattcattg agttggaata aatgcttctt aacacatctt ctttccctga ggaagaaaat
     6421 aaaaaatcaa gacatttctg attaaaagac gttgcaaagt tttctttttc agacactgat
     6481 gctggtgttt ttagagaagt ttgcgaacta tggatatcgg atattttgct ttttaactcg
     6541 ctgtattctg ttgatgatgg tgaagttgcc ttatataaga atgttggcgc ttgagtatta
     6601 tttacattat gcatggcgca cctcagaaat ggagaaaaag tttatctgta tctgtacatg
     6661 agcttattgt actactcaaa acctttacta aattatcaaa aatactattg gcattactgt
     6721 atttttgaac taaagtttga agattatttt tcattgtttc atcttcggca gagaatccgg
     6781 cattccatgc ctgatatttt gcattatcta gcacaacctc gccatttcca cctagattat
     6841 ctaagctttt taacatattg tctattgggg tcatgtttat actgacaaca tatcccccgt
     6901 ttttttgaga taccttgccg attgttccac ctaattctgt aagccattta tttgcttgtt
     6961 cctgactaac agtattattt gctggatata gcggtttatc tttatatttt tccttgagtt
     7021 cttccaatgc ctttttaagc gagttgactt gtaatttcac ggagtttccg tcgttacctc
     7081 cgggagagat ccagccggca agactggaaa gaacagcgct aaaatcttga tacatttgag
     7141 tatatgaact aacggcatgt tcatatactt tcagatactg ttcattaata tcattgatgg
     7201 agtttgcaat tttagcccac agttctctat gagatatcat ttgatctcca tcaagctcgg
     7261 cttctttcgg agctgaatgt aataattctc ttgcgtcttt attaattgga tattcgtgcc
     7321 tggaaagaat atctaatagt tgagcggatt tatttacatc catgctaatc tgagatgaat
     7381 gtaatgctat ttcttctagc gatgttttag tcaacgtttt ttgtgaaagc tctttcttta
     7441 atgcctgatt tgttgttctg atattatgaa gggtgtcgtt gagcatagta agggaactta
     7501 caggatgaga actggtcgtt gtttttatat cagaattaac tgtttcggtt gatgaaccgt
     7561 tggtattgtt tggactgaat gatgaggtgg aaatactatt agtcagagtt gttatattca
     7621 tggttatttc cttacaggaa gagccatata ttattatggc tcttggatat attaagctcg
     7681 aatgttacca gcaatctgac tggctgtcga attctttgat tggttgatgc tgtcaattat
     7741 attcaataat ttttgtatta attgatttgt cgcttgggat gcttctttag atacttggga
     7801 tgcttcctct gcttgtttac tgctggcctg actgattagt tgttcttctt cttcaagagc
     7861 agatgcataa cgccctcctg atgtggatat attaccagca acggctgatg tttccattgt
     7921 tgcacggcca atgttttgtt tttgctgcgc agaaagggta ttgagctcgt aagttcttct
     7981 ctgggtgtca attttatcct gcaaagaaat atcgggagaa ctaagagaag ttttatgttc
     8041 agttgacagg gatatattat ctggcgccag tttattttta cctaaaaatt ttgtgctaga
     8101 gttagtttgt ggtgaggtga tatttgtatc tatttgttta tttaacccta atttagaacc
     8161 tgcaagctct ttttcaagag attgagcagt ggcaaggttc tttcttaagg ctcctttttg
     8221 gtcgctaatc cctgaatgcg tttttttggc accgataccc gttataccta cttgtgtgac
     8281 tgctccagta atgcttgatg atagggctgc caggccttgc cgaacaatgt tctctgcagc
     8341 tgattttgta gcatcgaacg caatcaatga caattgagag cccaatttag tttctgcagt
     8401 gcggagagca gaaagtagaa cggctacact aataattaaa gaaacagcat tagaagaaag
     8461 actggaaata tccagagtgt tctctggagt aagggtgtga ctcatctctg aaagatcttt
     8521 catctttttg tcagtaaggc gagcaatgat ttctgaatca tgctgaacct gctctgataa
     8581 ctttaaaagt tgatcatcat ttaatgtggt tgttaatacg ggatttttac cgacattaag
     8641 tggaatatgc gctgcaatct gctgataatt ttgtgatttt tgtgtttcgg aagaactttg
     8701 agtttgtttt gtggatatat ctgtatataa aatctgggtt ggttttgtgt tttgaatttc
     8761 cataacattc tccttatttg tatcaagcag tagtttgttg caaaattgct tttgcaacat
     8821 cagttctatt agcctgagag ttggacattg aggctaatag atctgcaatt acttcctgca
     8881 attggccgaa tttttctatt gcttcactga tatattttga cagttgttca acttgatatt
     8941 tcgataatgt caggtctgct agatttgtcg acgcgctgtt ctggaaaaca gcagaagcga
     9001 cacttcctcc cgcttgtgtg gcagagttaa cactttctcc taataaaact gcttggttta
     9061 aatgggtgga aataatttgt ttggatatta cttcatcacc cgctgcacca agaaatttat
     9121 ttaatctggc aacagcatta gtgattaaat cgtccagttg agaagaaaaa ttcttgagaa
     9181 actttggtat aaggtctgtg agggttttac ctattatttt gccaatattt tctgcaagtt
     9241 ttgctgctgc ctgtttacca acagtggcta cgagaacgac tgctgcaact aggacaagag
     9301 cgcctgcgat tgcccccaga atagagccaa tcattttggc ttttttcgag tcgacgccca
     9361 agccttcgag catttttgta aatgcatctg aaaggagttt gattaagggt tcaatgactg
     9421 ctttcatgat cggattcagg gcttgttcca tgaaggaatt gccggtcgct gcttgtacta
     9481 tagcatccgt aaccataaga gctaaaccaa cagctgccag tgctagagag gctcctccag
     9541 aaaaagctgc tgcaacaaca ctaacgatag ttaaaagtgc cccaagtatt ttcccaacac
     9601 aacccattac tctgttgagt tcttctgctt tacgtacttc agcagcatac tcatcagatt
     9661 ttctctccat ttcagttttt cttgattctt ggagagactg gaatagagcc agatcatttt
     9721 ttaaagattc ttcattattt tttccaacta gttgaataaa ggttgccatc aattgagtaa
     9781 cactggcaag tccggttaat gatttctgct gggttgatag ctgttcagca gatgctgtgt
     9841 ttgaaaatgc agaaaaagag tctatttctt tttcgagttg cattgattta tctgtaagtt
     9901 tgctatgaat tgacagggtt ttctgctcaa tcaatgtcct gtctttaact gctgcgtctt
     9961 ttttgatagt gagttgtatt tcttcccggc ttaatttttt cttttctggt gactctgggt
    10021 cgagttcgga taatcttgtt tgaatttggt taattttatt ttctaggtct tttattttag
    10081 aatctgcgtt ttttagttta ttaatttgtt tttcatagtc tctggttagt ccttcagttt
    10141 cagatagaag agtgttaatt ttatcggaga attctaggtt tttttgctgt cttgcctgtt
    10201 gctgggactt ccaagcagta attttatttg ttaatgcagt taaagatttt tcaccgagta
    10261 tttgaataag gtttccaatt aaaagcgtta gttgggaact tgcatttaat gactttggtg
    10321 ctttaagttc agggattaat atatttgaag ttgagtgtgc attagttgta ttaatatttt
    10381 ggttagcagt aagatcagca attgtaagag aaaataattt gttagctgca tcatttgcag
    10441 cttggatagt attgtctcca agctcagtgg aagccaatat tttggcaaga ggaaaaccag
    10501 tggttgtggt gcttacatta tgcataataa ttactccttg atatcctgaa ttgcgtccaa
    10561 gtatgattgt gcttttattt ttaatttttc atcattgctg tgttgaatta cgagttcgaa
    10621 gcactcttta gcttttaagg gggctttcaa ccgaagctgg cattgtccag tatggaatac
    10681 tggtgtatag tcattttttc ctaatgcaaa agcgacagca taaaggtctg ctgcttgttg
    10741 gaactgttct tttatctgat aaatagctgc gagtcccata atgtagtcta cattgtaaaa
    10801 gtcgtatata cataaaaacc tgaagaaaac ttcagcttcc tctattcttc ctttgttgta
    10861 aaagtcataa gcatatgaat aaatgtcatc catcatatca tcaggaattg cattaatatc
    10921 tttcagtgta gcgccagagt taattgcatc aattactgca gtagagatgc tttcattttc
    10981 ggtgatattt aaagacataa ggtctccttt ttgtctgctt tactttaata agtataagat
    11041 ttaatataaa agatttaatt tgtattgctt tgacggtata cagcctttag aacctctgcg
    11101 cttatttcta tttgtaaaac ttttgttgat tcattaaata ttttatcgta gacggaactg
    11161 gatactagat ttgtaagatc cttcatttgt gtgttaaaca aatccatgtc actgctatag
    11221 cgttttaaaa tctttgctct gaggccagat gaggcaatct cgtttagtgc tgtatgactg
    11281 atagatgttt gtgctgattt ttcatcaagt tttatatccg ggaatttttt ttcaatagct
    11341 gcaaatatgt tttttttgtt atctgaggtc aaaacaactc cattgtgtat ggccatttca
    11401 tctatacttc tgtttatcat tgaagagaca taagtggttc gctcttggct catccaacaa
    11461 aatagttgat tccccgaatt actatctttt attttgtcta tgacgctgtc cctgatttta
    11521 tttatattca ccgggcatcc aaaattcttc tgaggaaagc gttgttttaa gttaaaaaca
    11581 gataatattt tctttacaag cttattgcga cttaacgaat caaatgatgg gcgaggaatc
    11641 gcgaattcaa cctgtggaag tattttgttt agaatttgca tgataccccc tatatgttag
    11701 ttcacttctg aagtgatgtt tgcaatggtt agtttaatac aaaataaaaa ttcatacaaa
    11761 gattcaatga gttcatcagg tgaaagatat tttttgtagt gtttcttggt caaaacacat
    11821 tgatgagtat cagggagaag acagatagaa gaaccataat ctctactcaa agagtagtta
    11881 aggatgagta tgtgttctgg attaataata ttttctgtaa aaagttcatg ttcattaaga
    11941 agtccggtca aaaagatgta ttcatcatta tctgttatac aaacaggtat ttgttcttcg
    12001 agcaatataa aggcttgatt tttatcgtta aaatcaagct ttgcacctgt tatttcataa
    12061 agtttatcat atagtttgcg acacatttct atggccttct atatattaga atgagagtta
    12121 ttcaataaaa gcttattgat ctgtaataag tcgtgaatgt aaagtttggt tccaagatct
    12181 ggcgatttaa gagaattgta ataatcaata atttttattg atttttcgat gatttcttct
    12241 tttttatctg ttgcttcggt ttttaattcg tttaatacac gagtcatatt gctgggtgct
    12301 gattttacta agtatgagtg gcaagcgttg atgaccgcat caaagtcttc tgaagataca
    12361 ttttcagtag gatgtttgta aagtgaaatt gataaaggct tgaatgtttt tggtgtgtgg
    12421 ataaggttaa ttttattgaa acttttaaga tagctgcgtc gtaattgagt caatctggaa
    12481 agatcaaaat cagattcaat atacttcatt atttcgttat aagactgatt gagactttca
    12541 atgcgttgcc taactttatt tgcatacgcg tatacatcgt taggtgtagc aaccaattta
    12601 acgtcaggga gtggaaagta tatttcagaa tttcctgctt taagaacatt aagtgccata
    12661 ccagcacagt tttcagattt actgattaat ttataatttg ccaattgtct gaaagcatct
    12721 gctttctttt tgcatataaa ttttgacatg tttgtttcat caaggccaaa aaggttatgg
    12781 ctgattttac catcctttgt tttattatca cctgataatg gaatgtatac tttatcagca
    12841 ctgacccccc aataaccata ggggtctttt ttttgattag ctctgggaaa aaatattttt
    12901 ttgttgttat tttgatcttg ttttgaattt aataagtttc taacctcttg gcctgcatca
    12961 agtctttcgg ctgtttggtc tgaaatcata tttagtttgt cttgcttata ggtatcgaca
    13021 ctgtaacctg agcgtttttc aaaaaggtag tttatttctg tatcttttgt aacattcccg
    13081 ccgggatacc agctgagata ggtatgagtt ggttcctgta gtaaattgtt ttttatactg
    13141 atactagtat gccctaaatg ggttatgcct ctatttcccc agcaacgaaa aactaaatgt
    13201 ctttcaagat ttttggctgt accgatgcca tgaaaacgct ctgcggtatt caacattaat
    13261 ttgatgtctc tacctgtgaa agagtgttga attgagtttt tagctgcttc ataggcggca
    13321 aacgcacttt ctgcagattt ttcgctagct aatgtattga ggaaaagcaa taaggatttt
    13381 tgattctcta gtcgataatt ttttagttgt tcatttaata tctgtgaatt tatctttgca
    13441 ttaatagtgc ttaatgttct gaaaaagaga tcctttaggt tgaatttctg tgctatcaat
    13501 ataggtccat gctctgtatc ttcaacgcgt ataggccctt ttgtattgct tgcgtcaatg
    13561 aaattgctaa ttttgaggat catactttat taactctcca ttacttggtg attgtgggga
    13621 tgtaaaatgt atttggtata taatatattc aattaataaa tttagaaact tgagcctgtt
    13681 aacataatca aattttcttt tgctgtacat aatatgtacc tcgtgagcat atgtagtgct
    13741 cgtttcatca tgaaatccca caagataaag tgcctgatgt atcaggctcg gagtgttata
    13801 gaaaaagaga gaaccctgtt gaataagtat cttttttgtt gtaaaacgag gtacaaaact
    13861 gcaatcaatg ccccatttat gactgaggat aattaaatgc acataactaa tttgggattg
    13921 catcaggttt catttcaaag cggagattcc tataaaggcg cagaagaaac cggaaagcac
    13981 aaaggtgtaa gcgtgatttc atatcaaaga gttaaaaatg gagaaagaaa taaggggatc
    14041 gaagcgctta atcgattata tttacagaac caaacaagct taacaggaaa aagccttttg
    14101 tttgcgcgtg acagagctga agttttttgc gaggcaataa agttggcagg tggtgatacg
    14161 tcaaaaatta aagccatgat ggaacgatta gatacctata aacttggtga agttaataaa
    14221 agacatatta atgagcttaa taaagtaata agtgaagaga taagagcaca gctaggcatt
    14281 aaaaataaaa aggaattgca aacgaaaata aaacaaatat ttactgatta tttaaataat
    14341 aagaattggg gaccggtcga caagaatatt agtcatcatg ggaaaaatta tggttttcaa
    14401 ttaactcctg cctctcatat gaagataggg aataaaaata tatttgtcaa agagtataat
    14461 ggaaaaggaa tttgttgtgc ttctacgaga gagagtgatc atatcgcgaa tatgtggctt
    14521 tcaaaagtgg tggatgatga ggggaaggaa attttttcgg gtatcagaca tggagttata
    14581 tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgctgctcg taataaagct
    14641 gaggagttag taagcgcagc attatatagc agaccagagt tattatcaca ggctttgtct
    14701 ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac cagtttaacc
    14761 gggggagagg agagtatgtt aaaagatcag gtgaacgcat taaaagggtt aaactctaaa
    14821 agggggaagc caacgaaatt attaattcgg aatagtgatg gtcttctgaa agaagtaagc
    14881 gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct aaaaatgggc
    14941 ttaggctgga ggaatgttga caagcttaat gatgaatcaa tatgttcttt gttgggggat
    15001 aatttcctca aaaatggtgt gattggaggt tgggctgcag aagcaataga aaaaaatcca
    15061 ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattgt aaccaaaaag
    15121 ttacaaaaaa atgataatgg agagccgtat aaattgtcac aaagaatgac tctattggct
    15181 tatactatag gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag aacaggtatg
    15241 caggatgctg aaattaaaag agaaataata agaaaacatg aaacaggtca gttttctcaa
    15301 ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct aatgaatagt
    15361 ggtaatatgg aaatccaaga gatgaatact ggtgtgcccg gaaataaagt catgaaaaaa
    15421 ttgccgctat cttcgttaga gctatcttat tctgaaagaa taggggaccc aaaaatatgg
    15481 aatatggtga aagggtattc gtcatttgta taagaggaat atatggaaga tttagcagat
    15541 gttatttgcc gagccttggg tatcccttta attgatatcg atgatcaagc aatcatgctt
    15601 gatgatgatg tgctaatata tattgaaaaa gaaggagatt caataaattt attatgccct
    15661 ttttgtgcat tgcctgaaaa tataaatgat cttatatatg cgttgagcct aaattactca
    15721 gaaaagatat gcttagctac tgatgatgaa ggcggaaatc taattgcgcg attagatctt
    15781 accgggataa atgaattcga ggatgtatat gttaatacag aatattatat ttcgcgtgtg
    15841 cgttggctga aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc cgtttcgtat
    15901 ttattttgct gtgttttatc ccatatttag ggagagctga ttgttgggat aaggctggtg
    15961 aaaggtacaa tattccatca agtttgttaa aagcgattgc ggaaaaagaa tccggattta
    16021 ataaatctgc agtcaatgtt aataacaatg gaagtaaaga ttatggtata atgcaaataa
    16081 atgactttca ttctaaaaga cttagagaaa tgggatattc tgaggaaatg ctaattagtc
    16141 atccatgtct ttctgtacat tatgcagcaa agttattaaa tgagtttatg atgatgtatg
    16201 gaagagggtg ggaggctgtt ggagcctata atgcaggaac gtctccgaaa aagaaaaaag
    16261 aaagattaaa gtacgcagag gatatctata gacgctattt gcggatagca gcagagagca
    16321 agcagaataa tcgaaggata taagaggatt tatgtctgag gcaaagaact caaatcttgc
    16381 accattcagg ttattagtga aactcacaaa cggtgtcggg gatgagtttc cgttatatta
    16441 tgggaataac ttaattgttc tcggacggac gatagaaaca cttgagtttg gaaacgacaa
    16501 tttcccagaa aatattattc ctgtaacaga tagtaaatcg gatggtataa tttatttgac
    16561 cataagcaaa gataatatct gtcaatttag taatgaaaaa ggtgagcaaa ttgatatcaa
    16621 ttcacaattt aattcatttg aatatgacgg gatttctttt caccttaaaa atatgaggga
    16681 ggataaatcg agaggccata tactaaacgg aatgtataag aaccactcgg tgtttttttt
    16741 ctttgctgtt attgttgtgt taattataat tttttccctc tctttaaaga aagatgaagt
    16801 aaaagaaatt gctgaaatta tagatgataa gagatatgga atagtcaaca ctggacagtg
    16861 caattatatt cttgccgaaa cacaaaatga tgcggtatgg gcgtctgtag ccttaaataa
    16921 aaccggattt accaaatgca gatatattct ggtgtcaaac aaagaaataa atagaattca
    16981 acaatatatc aatcaacgat tcccatttat caatttgtat gtgttgaatt tagtgagtga
    17041 taaagcggaa ttactggtgt ttttaagtaa agaaagaaat agttcaaaag ataccgaact
    17101 tgataaattg aaaaatgctc tgattgttga atttccgtat atcaaaaata ttaaattcaa
    17161 ctatcttagc gatcataatg caagggggga tgctaaaggt attttcacaa aggtaaatgt
    17221 ccagtataaa gagatttgtg aaaataataa agttacatat tctgtcagag aagaactgac
    17281 agatgaaaag cttgagctca ttaatagact gatttctgaa cataaaaata tatatggaga
    17341 tcagtatatt gagttttctg tacttttgat agatgatgat tttaaaggta aatcatatct
    17401 taacagcaaa gacagttatg taatgttgaa tgataaacac tggttttttt tagataaaaa
    17461 taagtgagga taaaatgagt gttacagtac cgaatgatga ttggacattg agttcattat
    17521 ctgaaacttt tgatgatgga actcaaacat tacaaggtga actaacattg gcactagata
    17581 aattagctaa aaatccttcg aatccacagt tgctggctga ataccaaagt aaattatctg
    17641 aatatacatt atataggaac gcgcaatcca atacagtgaa agtgattaag gatgttgatg
    17701 ctgcaattat tcaaaacttc agataatagg gagcattcat gaattacatt tatccagtca
    17761 atcaggttga tattatcaaa gccagtgatt ttcaatctca agagatatca agtctggaag
    17821 acgtcgtgtc ggctaaatat agtgatatta agatggatac agatattcaa gtatcacaaa
    17881 taatggagat ggtaagcaat ccagaatcat taaacccaga atctttggcc aagttacaga
    17941 cgacgctctc aaattattca ataggagtat cattagctgg cacgttagca agaaaaacag
    18001 tttcggctgt tgaaacttta ttaaagtctt aatttatatg attaggtata aaggttttat
    18061 tttattcttg ttgctgatgt tgattggatg tgagcaacgt gaagagttaa tttctaattt
    18121 atctcaaaga caggcaaatg aaataatatc tgtgctagaa cgccataata ttactgctag
    18181 aaaagttgat ggaggtaaac aggggatctc ggtacaagtc gaaaagggga catttgcatc
    18241 ggcagttgat ttgatgcgca tgtacgattt gccaaatccg gagagagttg atatctcaca
    18301 aatgtttcct acagattcat tagtgtcttc tccaagagct gaaaaggccc gtttatatag
    18361 tgctattgag caacggctgg aacagtcttt agtttctatt ggtggtgtta tttcggcaaa
    18421 aatacatgtt agctatgatc ttgaagaaaa aaatatatct tcaaaaccga tgcatatatc
    18481 agtaatcgct atatatgact caccgaaaga gtctgaacta ttagttagta atattaagcg
    18541 atttttgaaa aacacctttt ctgatgttaa gtatgaaaat atatctgtca tattaactcc
    18601 gaaagaagaa tatgtttata caaatgtaca acctgttaag gaagttaaat cggaattttt
    18661 aacaaatgaa gtaatatatt tatttctcgg gatggctgta ctagttgtca ttcttttggt
    18721 atgggcattc aaaacagggt ggttcaagag aaacaaaata tgataagaat ggatggaatt
    18781 tataaaaaat atctttcaat aatttttgat ccagcgttct atataaatag aaatcggttg
    18841 aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa gtgagattaa taatctcata
    18901 attaataaat atgatctaaa ttgcgatatt gaacctttaa gcggggtaac cgctatgttt
    18961 gttgccaact ggaatttact tccagctgtt gcttatttta tcggtagtca agagtctcgg
    19021 ttaattaatc atagtgagat ggttattagt tattatgggg gcaaaatcag taagcaaggg
    19081 gaggccgcta ttcgaagtgg tttttggcat cttattgcgt ggaaggaaaa tatatctgtt
    19141 ggtatatatg aaagaataaa cttattattt aatccaattg ctttggaggg aaattatacg
    19201 cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc aatatgcaaa aaggcacttt
    19261 accggtatcc agacatcatg cctatgatgg tgttgttata aagagaattg aaaaggaatt
    19321 atgtaaaacc ataaaagata gagacactga gagtaagaag aaagcaattt gtgttataaa
    19381 ggaggctacg aaaaaagctg aatcactgcg tatagatgct gtatgtgatg gttatcaaat
    19441 tggtatacag actgcttttg agcatattat tgactatatt tgtgaatgga agttaaagca
    19501 aaatgaaaat agaagaaata ttgaggacta tataactagt ttgctttcag aaaatttaca
    19561 tgatgagagg atcattagca ctttattgga gcagtggctt agttcgttgc gtaatacagt
    19621 aacagaatta aaagtggttt tgcccaagtg taatttagca ctgaggaaaa agttagaact
    19681 tgatctccat aaatatcgct cagatgtcaa gattatttta aaatattctg aagggaataa
    19741 ttatatattc tgtagtggta atcaagtagt ggagtttagt cctcaggatg taatatcagg
    19801 tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat aaaaaatatt tcaaagagtt
    19861 agcacataag aaactgagac aaattgcaga agacttgtta aaagagaatc ctgttaatga
    19921 ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt aaatagtgaa gaagttgtta
    19981 atttaaatga gagattatca tcttcgcaat catttgatga ggatataatc tatgaaataa
    20041 tgcaatattt ctcacagagt gaattaaatt ccatagataa tgatgaactg cacaataaga
    20101 ttgaacaatt attcaattca cgttttcctt atcttacagc tgcacaaaaa agttcacttc
    20161 ttaataaact catagatgca aatcaatatg tcgatttgca tgagggattt tatgcatcat
    20221 tgtccatata taacaatatt gatttctata ttaagactac aaccttcgat tcattaatta
    20281 gtgtctttga agcagggaga gaggcagatg attcgacatg gtagtaataa gttgaaaata
    20341 tttattttaa gtatattgct attaacactg agtgggtgtg ctttaaagtc atcatctaat
    20401 tctgaaaaag aatggcatat tgttcctgta agtaaggatt atttttctat tccaaatgat
    20461 ttattatggt cgtttaatac aaccaataaa agtataaatg tttactctaa atgtattagt
    20521 ggtaaggcgg tttatagttt taatgcaggt aaattcatgg gcaactttaa tgttaaggaa
    20581 gtagatgggt gcttcatgga tgcacaaaag atagctatag ataaactatt ttctatgctg
    20641 aaagacgggg ttgttttaaa aggtaataag ataaatgata ccatccttat agagaaggat
    20701 ggggaagtta aattaaaatt aattcgaggg atataattgt attgtgagta aatataaagg
    20761 tctaaataca agtaatatgt tttacattta ctctagtgga catgaaccag ttaacgttga
    20821 gcttgtaaaa gataaagaac gtaacataat tgagctggct ccagcatgga agggtttttt
    20881 tttgtccgaa atcaaaatat aaaattcagt gataatgtga actatcatta tcgctttaac
    20941 attaatagtt gtgcaaaatt tcttgctttt tgggactatt tcagtggtgc tctagtagag
    21001 catagccatg ctgaaaaatg tattcatttc tatcatgaga atgatcttcg ggatagttgt
    21061 aataccgaat cgatgcttga taagttaatg ttaagattca tcttttcatc tgaccagaat
    21121 gtaagtaatg cactggctat gatacgaatg actgagtcat atcatttggt tctttatctt
    21181 ctgagaacta ttgagaaaga aaaggaggta agaataaaga gtctgacaga gcattatggt
    21241 gtgtcggagg cttattttag atctttatgt agaaaagctt taggggcaaa agtcaaagaa
    21301 caactgaaca cttggcggtt agtaaatggt ttattagatg tatttttgca caatcaaaca
    21361 atcacttcag ctgccatgaa taatgggtat gcatcaacat cccatttttc aaatgagata
    21421 aaaacacgac taggcttttc tgcaagagaa ttatcgaata ttactttttt agtgaaaaaa
    21481 ataaatgaaa aaatttaata ttaaatcttt gactctcttg attgtattgt taccattgat
    21541 agttaatgct aataatattg actctcattt attagaacaa aatgacatag cgaaatatgt
    21601 tgctcaaagt gatacggtag gatctttttt tgaacgtttt tctgcgttat taaattatcc
    21661 tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct ggggagtttg atttatctaa
    21721 tccagaagaa atgcttgaaa aactgacatt acttgtggga ctcatatggt ataaagatgg
    21781 aaatgcatta tacatatatg actctggaga attaataagc aaagttattt tgctggagaa
    21841 tatatcactt aattatctta ttcagtatct gaaggatgct aacctttatg atcacagata
    21901 tcctataaga ggaaacatta gtgataaaac attctatata tctggtcctc cggcattggt
    21961 tgaactggta gctaatacag caactttgct tgataagcag gtttcttcta ttgggacaga
    22021 taaagttaat tttggggtta taaaattaaa aaatacattt gttagtgaca gaacttataa
    22081 tatgagaggg gaggatattg tcatcccagg ggtggctact gttgtagaac gtttattaaa
    22141 taatggtaag gctctgagta acagacaagc ccaaaatgat ccgatgccac cctttaatat
    22201 tacacagaaa gtatctgagg atagtaatga cttttcattt agttcagtta ccaatagttc
    22261 tatattagag gatgtatcac ttattgcata tccagaaacc aattctatat tagtcaaagg
    22321 taatgatcag caaatacaga ttattcgcga tattatcact cagttagata tagccaaacg
    22381 acatatagaa ttatcattat ggataatcga tattgataaa tctgaattaa ataaccttgg
    22441 tgttaattgg caaggaacgg cttcatttgg tgattcgttt ggtgcgtcat ttaatatgag
    22501 ctcatcagca tcgataagca ctttggacgg caataagttt attgcttcag ttatggcatt
    22561 aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg attttgacac aagagaatat
    22621 tccggctata tttgataata acaggacttt ttatgtgtca ttagttggtg agcgcaattc
    22681 ttcgttggag catgttacgt atggaacatt gataaatgtt atacctcgct tttcatctcg
    22741 gggacaaatt gagatgtctt taacgataga ggatggaaca ggaaatagtc agtcaaatta
    22801 caattataat aatgaaaata catctgtcct tccagaagtt ggaaggacca aaatcagtac
    22861 aatagcgaga gttccacaag gcaaaagttt gttgataggt ggttatacgc atgaaacgaa
    22921 ctctaacgaa ataatatcta ttccttttct ctcctctatt cctgtaatcg gtaatgtgtt
    22981 caagtataaa acaagtaata tcagtaatat tgtaagggtt tttctgattc aaccccgtga
    23041 gataaaggag tcaagttatt ataatactgc agaatataaa agcctcatat ctgaaagaga
    23101 aatacaaaag actacacaaa taatcccatc tgaaactact ttgctggaag acgaaaaatc
    23161 attggtttca tacttaaatt actaactata aagtaggtga tgtatgcttg atgttaaaaa
    23221 tacaggagtt tttagctctg cattcattga taagttgaat gcaatgacaa attcagatga
    23281 tggagatgag actgctgatg cagagcttga ttctggcttg gctaatagca agtatattga
    23341 ctcatctgat gagatggctt ccgctctttc gtcatttata aacagaagag accttgagaa
    23401 actgaaagga acaaatagtg atagtcagga acgtatttta gatggggaag aagatgaaat
    23461 taatcacaag atttttgatt taaagagaac gttaaaagat aaccttcctc tagatcggga
    23521 ttttatagac agactaaaga gatattttaa agatccaagt gatcaagtct tagcattaag
    23581 ggaacttttg aatgaaaaag atcttactgc tgaacaagtc gaattattaa ctaaaattat
    23641 taatgagata atatcaggta gtgaaaaaag tgttaatgct ggaataaatt cagctataca
    23701 ggctaaatta tttggcaaca aaatgaaact tgaaccacag cttttgcgtg catgttatcg
    23761 tggttttatc atggggaaca tatcaacaac agatcagtat atagaatggc ttggtaattt
    23821 tggttttaat cacagacata caattgtgaa ttttgtagag cagtcactga ttgtagacat
    23881 ggattctgag aaaccgagct gtaatgctta tgagtttggt tttgtgttat ctaaattaat
    23941 tgcaattaag atgattagaa cttcagacgt aatttttatg aagaaactgg aatcctcaag
    24001 cttgctaaaa gatggcagtt taagtgcaga gcagctattg ctaactttat tatatatttt
    24061 tcaatatcca agtgaaagtg agcaaattct tacttctgtt atagaagtat cacgagccag
    24121 tcatgaggat tctgtagtgt atcaaacata tctatcttct gttaatgaaa gtcctcatga
    24181 tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat attctacgag agcttgtcac
    24241 aagtgcatac aagaaagagc tttctagata acaggagata aaagtgatcc agtcttttct
    24301 taagcaagta agtactaagc ctgaattaat tatattagtg cttatggtta tgatcattgc
    24361 tatgttgatc ataccattac ctacatacct tgttgatttt ttaatagggc ttaatattgt
    24421 acttgctatt ctcgttttta tgggatcatt ttatattgaa agaatattga gtttttctac
    24481 attcccttcg gttttgttga taacgacatt atttcgtctc gctttatcaa taagtactag
    24541 tcgactaatt ttagttgatg cggatgcggg taaaattatt actacatttg gccagtttgt
    24601 cattggtgat agtctggctg taggttttgt tattttttca atagtaactg ttgtgcagtt
    24661 cattgttata acaaaaggat ctgaaagggt tgcggaggta gctgctcgct tttctcttga
    24721 cggtatgccg gggaaacaaa tgagcattga tgcggattta aaggctggaa ttattgatgc
    24781 cgcaggagct aaagaaagac gcagtattct ggaacgtgag agtcaattgt atggttcatt
    24841 tgatggagca atgaagttca ttaaaggtga tgcaattgct ggcatcatta ttatttttgt
    24901 caatttaata ggaggtattt ctgttggtat gagtcaacat ggaatgtccc tatctggtgc
    24961 attatctact tataccatac taactattgg tgacggattg gtctctcaga ttcctgctct
    25021 gttaatttct ataagtgcag gatttatcgt tacaagagtt aatggtgata gcgataatat
    25081 gggacgtaac ataatgtccc agatatttgg gaatcctttt gttctcattg ttacatcagc
    25141 tcttgccttg gcgataggga tgttgccagg ttttccgttt tttgtttttt tcctgatagc
    25201 agttactttg acggctttat tctattataa aaaggtcgta gaaaaagaaa aaagtctgtc
    25261 tgagtctgat tctagtggct atactggtac atttgatatt gataatagcc atgactcatc
    25321 tttggcaatg atagaaaatc tggatgctat tagttcagaa accgttcctt taattttatt
    25381 atttgccgaa aataagataa atgcaaatga tatggaaggt cttattgaaa ggataagaag
    25441 tcagttcttt attgattatg gtgtcaggct tccaactatt ttatatagaa caagcaatga
    25501 gcttaaggtc gatgatattg ttttgttaat aaatgaggtg cgtgccgata gttttaatat
    25561 atattttgac aaagtgtgta ttacagatga aaatggagat atagatgctc taggtattcc
    25621 tgttgtttca acttcatata atgaacgtgt tatttcttgg gtagacgttt catatacaga
    25681 aaatctaact aatattgatg ctaaaattaa aagtgctcaa gatgaatttt atcaccagtt
    25741 gtcacaagct ttattaaaca acataaatga gatttttggt atacaagaaa caaaaaatat
    25801 gttagatcag tttgaaaatc ggtatcctga tctattaaag gaagtcttcc gacatgtgac
    25861 tatacagaga atttctgagg tattacaaag attgcttgga gaaaatattt ctgttcgcaa
    25921 tttaaaactt attatggagt ctttggcgct ttgggctccc agagaaaaag atgtcataac
    25981 attagttgaa catgtccgtg catcactttc taggtatatt tgtagtaaaa tagctgtttc
    26041 tggtgagatt aaagttgtga tgctttccgg atatattgag gatgcaataa gaaaagggat
    26101 aaggcaaacc tctggtggct ctttcttgaa tatggatata gaggtttcgg atgaggtaat
    26161 ggaaacttta gcacatgctt tgagagaatt gagaaatgca aaaaaaaatt tcgttctttt
    26221 ggtatcagta gatatacgta ggtttgttaa aagacttata gataacagat ttaagagtat
    26281 actcgttata tcgtatgctg agattgatga agcatatacc attaatgtat taaagactat
    26341 ttagtgaggt ttaaatatga gtaacattaa tttagttcaa ttagttagag atagtctttt
    26401 cacgattggt tgtcctccat caattattac agatttagac agccattcag cgattaccat
    26461 ttctttggat agtatgcctg ctattaatat agctcttgtc aatgagcagg ttatgttatg
    26521 ggctaatttt gatgcaccaa gtgatgtaaa acttcagtca tctgcttata acatattaaa
    26581 tttaatgtta atgaacttta gttatagcat taatgagctg gtagagcttc atcgttctga
    26641 tgagtactta cagttaagag ttgttattaa agatgactac gttcacgatg ggattgtttt
    26701 tgcagagatt ctgcatgagt tttatcagag gatggaaatc ttaaatgggg tcttataatc
    26761 aatgagctat acaaaattgc tcactcaatt atcttttcct aatagaatct cggggccaat
    26821 cttggaaaca agtcttagcg atgtttcgat tggtgagatt tgtaacattc aggctggaat
    26881 tgaaagtaat gaaattgttg caagagctca ggttgtagga tttcatgatg aaaaaacaat
    26941 attaagcttg attggaaatt ctcgtggact ttcacggcaa acgttgatta agcccactgc
    27001 ccagtttctt catacgcaag tcggccgtgg attattggga gcagtagtca atcctttagg
    27061 ggaggttact gataagtttg ctgttacaga taacagtgaa attctttatc gacctgtaga
    27121 taatgctcct ccgctatata gtgaaagggc tgcaattgag aagccttttt taacaggtat
    27181 taaggttatt gattctttac tcacgtgtgg tgaaggacag cgaatgggga tttttgcgtc
    27241 agctggttgt ggcaaaactt ttctcatgaa tatgctcatt gaacatagtg gtgctgatat
    27301 atatgttatt gggttaattg gtgagcgagg tcgagaggtt actgaaacgg ttgattattt
    27361 gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat gcaacttcgg attactcttc
    27421 ggttgatcgt tgtaatgctg catatatagc cactgctata gccgaatttt ttaggactga
    27481 aggacataaa gtagcgcttt ttattgattc attaacaagg tatgccagag cattacgtga
    27541 tgtggcctta gccgctggag aatcacctgc cagaagaggc tatccggttt cggtttttga
    27601 tagcttaccc agacttcttg aaaggccagg aaagttaaag gcaggtggct ctattactgc
    27661 attttacact gttcttttgg aggatgatga ttttgctgat ccattagctg aagaggtaag
    27721 atccatttta gatggacata tatatttgag cagaaatcta gcccaaaaag gacaatttcc
    27781 tgcaattgat tccttaaaaa gtataagcag ggtatttaca caggttgttg atgaaaaaca
    27841 tcgtattatg gccgctgcat ttcgggagct actttctgaa atagaagagc taaggacaat
    27901 tattgacttt ggtgaataca aaccggggga gaatgcctct caggataaaa tctacaacaa
    27961 aatatctgtt gttgagagtt ttctgaagca agattatcga ctcggtttta cttatgagca
    28021 gacaatggag cttattggtg aaacaattag ataaggtttt aaaaatcaaa gataaatatc
    28081 aacggtctgt taaattaatt gaagcacaca tattaacact aaaaaaaaaa ctctttgtat
    28141 cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga gaatgactta
    28201 gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa gatcgctata
    28261 gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg aagaattgaa
    28321 gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct tcataaagag
    28381 agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa acagttgata
    28441 cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa acctaaattt
    28501 tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata atattgatag
    28561 tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa ttgcatctaa
    28621 ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa tttatagtgg
    28681 cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata ttccagaatc
    28741 aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa gggaaaaaat
    28801 aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg cagacaagaa
    28861 cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat ctgataatag
    28921 aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata ttgctgacat
    28981 catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa tgaataaaaa
    29041 tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg aaaatgataa
    29101 aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg gggctggaca
    29161 ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag atcaatttgt
    29221 aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca gatttgatag
    29281 cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac agagtgaaga
    29341 agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt atatgcaaag
    29401 caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg cagtgaaagt
    29461 ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac attatctttt
    29521 ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt actgcattta
    29581 tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact gtctgataag
    29641 attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca tttatgctgt
    29701 ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg taatcaagtt
    29761 tctcttgact cattgcatca tcttttagag ttttgcttag gttcatctaa tgtaaggctg
    29821 gctactttaa aaagaattcg cactggtgat ataatcatag ttcagaaact ttataattta
    29881 ttattgtgta atcaagttat tattggggat tatattgtga atgataataa tgaggcaaaa
    29941 attaatctgt cagaaagtaa tggtgagtca gaacacacag aagtttcttt ggcattattc
    30001 aattatgatg atatcaatgt aaaagtggac tttattcttt tagaaaaaaa tatgacaatc
    30061 aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga catagttaaa
    30121 catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt ttctattgag
    30181 gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca tgctgagtga
    30241 catgtccctc atcgctacac tttctttttt tacgctatta ccatttttag ttgctgctgg
    30301 tacctgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac ttggtttgca
    30361 gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt tgttcgtgat
    30421 gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggccctcaaa aatttgatac
    30481 aatatctgat attgtccgtt tttctgattc tggcttgatg gaatataagc aatacttgaa
    30541 aaagcataca gatcttgagt tagcacgttt tttccaaagg tctgaagaag aaaatgctga
    30601 tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg catatgcgct
    30661 aagtgaaatc aaagatgcgt tcaaaatagg tttttattta tatttgccat ttgttgttgt
    30721 tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga gtccaatcac
    30781 aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg gtatcctgtc
    30841 taaggccttg attgaacaat atatcaatat tcctgcttag atgatggagg taatgtgtct
    30901 gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc cttatggcca
    30961 gtaggtatag ctacggttat tggattatct attggtttgt tacagacagt gactcaactc
    31021 caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc tttgctactt
    31081 ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat gtttttaatt
    31141 aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg tttttaatac
    31201 tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca tttttaaata
    31261 acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca tcaggattaa
    31321 ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat ttccttatgt
    31381 tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc ttttggatat
    31441 ttcatgctgt tggtagcatt attgacaacc agcgtggggc aacgcttagt agttcaattg
    31501 atcctgccaa tggtgttgat acgtctgagt tggcaaaatt tttcaatctt ttttctgcag
    31561 ttgtatttct atacagtggt ggtatggtct ttattttaga atccatacaa ttgtcttata
    31621 atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta acatttctga
    31681 ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta ttgttactat
    31741 cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct ttttccgtat
    31801 cattaactat taaaagttta cttgcaatat ttattatttt catctgttct tctactattt
    31861 acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat ctatttgtta
    31921 gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta aaggatgccg
    31981 caaaaaaagg acagtcattt aaatttaagg atttaacgac tgttgttatt attctggtag
    32041 ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta tacagatatg
    32101 taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg gttattgtct
    32161 tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg ttgccaacat
    32221 tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt tcagtattaa
    32281 accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa tttttcaaaa
    32341 gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt aatgaccgaa
    32401 aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt tgggggaggc
    32461 tgtttaagga tataatatta tttttcttgg cattttctat tcttgttatt attcttgact
    32521 ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag gagataaaaa
    32581 gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt gagttgcata
    32641 tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta gtggtaatga
    32701 acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct gcacctttta
    32761 tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca aatgaagttg
    32821 gtataccgac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca catacaaaat
    32881 atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt tggcttgagc
    32941 aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt aatttctgta
    33001 ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt aactccattg
    33061 ctaatgaggt attgaaccca atatctttcc ttagcaataa ggatataata aatgttttac
    33121 tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt tgtgctctgg
    33181 agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact cttttgctga
    33241 tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag aaaattgtta
    33301 tgtttttgtt aggaactgca ctaaataata tatgataaga cagcaaaaac gactgaccat
    33361 aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg ttaaaactct
    33421 gttatatagt attcgcgatt atgcaaagtc tgtaaatgat catgagatac taaccgagag
    33481 taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta aaagtagtaa
    33541 atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac caaatgatta
    33601 gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt tttgagactg
    33661 taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg gagacaggta
    33721 aattatggac gaaaagaagc ttaaagccct tgcggctgaa ctggctaaag gcctcaaaac
    33781 tgaggccgac ctcaatcagt tttcccgtat gctgacgaaa tatcccggct cttaaaacga
    33841 tagtggctca ggctttcatt tttcagcgtt ccccagaag
//
LOCUS       DAJVQR010000038        33516 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVQR010000038 DAJVQR010000000
VERSION     DAJVQR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="OQM52_001915"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="OQM52_001915"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1563788.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="OQM52_001916"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="OQM52_001916"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1563789.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="OQM52_001917"
                     /pseudo
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="OQM52_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="OQM52_001918"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="OQM52_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1563790.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="OQM52_001919"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="OQM52_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1563791.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /gene="radC"
                     /locus_tag="OQM52_001920"
     CDS             complement(3421..4089)
                     /gene="radC"
                     /locus_tag="OQM52_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1563792.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="OQM52_001921"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="OQM52_001921"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1563793.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="OQM52_001922"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="OQM52_001922"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1563794.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="OQM52_001923"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="OQM52_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1563795.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="OQM52_001924"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="OQM52_001924"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1563796.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="OQM52_001925"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="OQM52_001925"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1563797.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="OQM52_001926"
     CDS             8207..9070
                     /locus_tag="OQM52_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1563798.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="OQM52_001927"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="OQM52_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1563799.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="OQM52_001928"
     CDS             10406..11023
                     /locus_tag="OQM52_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1563800.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="OQM52_001929"
     CDS             complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="OQM52_001929"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1563801.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12960..13583
                     /gene="gmk"
                     /locus_tag="OQM52_001930"
     CDS             12960..13583
                     /gene="gmk"
                     /locus_tag="OQM52_001930"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1563802.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13638..13913
                     /gene="rpoZ"
                     /locus_tag="OQM52_001931"
     CDS             13638..13913
                     /gene="rpoZ"
                     /locus_tag="OQM52_001931"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1563803.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13932..16040
                     /gene="spoT"
                     /locus_tag="OQM52_001932"
     CDS             13932..16040
                     /gene="spoT"
                     /locus_tag="OQM52_001932"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1563804.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16047..16736
                     /gene="trmH"
                     /locus_tag="OQM52_001933"
     CDS             16047..16736
                     /gene="trmH"
                     /locus_tag="OQM52_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1563805.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16742..18823
                     /gene="recG"
                     /locus_tag="OQM52_001934"
     CDS             16742..18823
                     /gene="recG"
                     /locus_tag="OQM52_001934"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1563806.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="OQM52_001935"
     CDS             complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="OQM52_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1563807.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20342..21733
                     /gene="xanP"
                     /locus_tag="OQM52_001936"
     CDS             20342..21733
                     /gene="xanP"
                     /locus_tag="OQM52_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1563808.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21854..23563
                     /locus_tag="OQM52_001937"
     CDS             21854..23563
                     /locus_tag="OQM52_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1563809.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="OQM52_001938"
     CDS             complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="OQM52_001938"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1563810.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25944..27327)
                     /locus_tag="OQM52_001939"
                     /pseudo
     CDS             complement(25944..27327)
                     /locus_tag="OQM52_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27620..27714
                     /locus_tag="OQM52_001940"
     tRNA            27620..27714
                     /locus_tag="OQM52_001940"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27654..27656,aa:Sec,seq:tca)
     gene            27986..29197
                     /locus_tag="OQM52_001941"
     CDS             27986..29197
                     /locus_tag="OQM52_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1563811.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29609..30652
                     /locus_tag="OQM52_001942"
     CDS             29609..30652
                     /locus_tag="OQM52_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1563812.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31167..31595
                     /locus_tag="OQM52_001943"
                     /pseudo
     CDS             31167..31595
                     /locus_tag="OQM52_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31625..32776)
                     /locus_tag="OQM52_001944"
                     /pseudo
     CDS             complement(31625..32776)
                     /locus_tag="OQM52_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32841..33489
                     /locus_tag="OQM52_001945"
                     /pseudo
     CDS             32841..33489
                     /locus_tag="OQM52_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8400 a         8809 c         8255 g         8052 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccggaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctac tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gatgcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacgcgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttcgc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gccgctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt aggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat gatttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgaag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccata cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg accgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gccccagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgggt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccggcaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 gttggctaaa ggcccgctaa cggtttgcag cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cgctacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccttccagt agtcatcgtc ccactgtttt atttgctgtt gcaggcggga
    12601 aatttcttcc tgtgctctgg ctggcgacca ggccggacag accgcccaca cagatgattg
    12661 ccagcacaag atacttatta atatcgccat ccatactttc atcttcacct ccgctgtagt
    12721 caggctggca gatatacaac gtggtgaagg caaagccgag tggcaaaaac ggagtctgcg
    12781 aggacgcttc ctgagaatcg tctttattgc agtaaatcac aggcaaatgc ggaaacagct
    12841 acgcaaaacg caacaacttt gcgcaaaaag tgtgagcaag ggctacgtca catggccgcg
    12901 ccgtgtataa taagctcgta tgtaggcttt atttcgctaa tcacatacga aagatactca
    12961 tggctcaagg cacgctttat attgtttctg cccccagtgg cgcgggtaaa tccagcctga
    13021 ttcaggcttt attaaaaacc caaccgttgt atgacaccca ggtttctgtt tcacacacca
    13081 cacgccaacc gcgtcctggt gaagtccacg gtgaacatta tttctttgtt aatcatgatg
    13141 aatttaaaga aatgattagc agagatgcgt tcctcgaaca cgcagaagtt tttggtaatt
    13201 actatggcac ttcgcgtgag gccattgagc aagtactggc gaccggtgtc gatgtttttc
    13261 tcgatatcga ctggcagggc gcgcagcaaa ttcgccagaa gatgccgcac gcgcggagta
    13321 tctttatttt accgccgtcc aaaattgaac tggaccgccg tctacgcggt cgcggtcagg
    13381 acagcgaaga ggtcattgca aagcgtatgg cgcaagctgt tgcagaaatg agccattacg
    13441 ccgaatatga ttatctgatt gtgaatgatg acttcgatac cgcgttgacc gatttgaaga
    13501 ccattattcg cgccgaacgt ctgcgcatga gccgccaaaa gcagcgtcat gacgctttaa
    13561 tcagcaaatt gttggcagac tgaacctgat ttcagtatca tgcccagtca tttcttcacc
    13621 tgtggagctt tttaagtatg gcacgcgtaa ctgttcagga cgctgtagag aaaattggta
    13681 accgttttga cctggtactg gtcgccgcgc gtcgcgctcg tcagatgcag gtaggcggaa
    13741 aggatccgct ggtaccggaa gaaaacgata aaaccactgt aatcgcgctg cgcgaaatcg
    13801 aagaaggtct gatcaacaac cagatcctcg acgttcgcga acgccaggaa cagcaagagc
    13861 tggaagccgc tgaattacaa gccgttaccg ctattgctga aggtcgtcgt taatcacaaa
    13921 gcgggtcgcc cttgtatctg tttgaaagcc tgaatcaact gattcaaacc tacctgccgg
    13981 aagaccaaat caagcgtctg cggcaggcgt atctcgttgc acgtgatgct cacgaggggc
    14041 aaacacgttc aagcggtgaa ccctatatca cgcacccggt agcggttgcc tgcattctgg
    14101 ccgagatgaa actcgactat gaaacgctga tggcggcgct gctgcatgac gtgattgaag
    14161 atactcccgc cacctaccag gatatggaac agctttttgg taaaagcgtc gccgagctgg
    14221 tagagggggt gtcgaaactt gataaactca agttccgcga taagaaagag gcgcaggccg
    14281 aaaactttcg caagatgatt atggcgatgg tgcaggatat ccgcgtcatc ctcatcaaac
    14341 ttgccgaccg tacccacaac atgcgcacgc tgggctcact tcgcccggac aaacgtcgcc
    14401 gcatcgcccg tgaaactctc gaaatttaca gcccgctggc gcaccgttta ggtatccacc
    14461 acattaaaac cgaactcgaa gagctgggtt ttgaggcgct gtatcccaac cgttatcgcg
    14521 taatcaaaga agtggtgaaa gccgcgcgcg gcaaccgtaa agagatgatc caaaaaatcc
    14581 tctcggaaat cgaagggcgt ttgcaggaag cgggaattcc ctgccgcgtc agtggtcgcg
    14641 agaagcatct ttattcgatt tactgcaaaa tggtgctcaa agagcagcgt tttcactcaa
    14701 tcatggacat ctacgctttc cgcgtgatcg tcaatgattc cgacacctgt tatcgcgtgc
    14761 tcgggcaaat gcatagcctg tacaagccgc gtccgggtcg cgtgaaagac tacatcgcca
    14821 ttccaaaagc gaacggctat cagtctttgc acacctcgat gatcggcccg cacggtgtgc
    14881 cggttgaggt ccagatccgt accgaagata tggatcagat ggcggagatg ggtgttgccg
    14941 cgcactgggc ttataaagag cacggcgaaa ccagcaccac cgcgcaaatc cgcgcccagc
    15001 gctggatgca aagcctgctg gagctgcaac agagcgccgg tagttcgttt gaatttatcg
    15061 agagcgttaa atccgatctc ttcccggatg agatttacgt tttcacaccg gaagggcgaa
    15121 ttgtcgagct gcctgccggt gcaacgcccg tcgacttcgc ttatgcagtg cataccgata
    15181 tcggtcatgc ctgcgtgggc gcacgcgttg accgccagcc ttacccgctg tcgcagccgc
    15241 ttaccagcgg tcaaaccgtt gaaatcatta ccgcaccggg cgctcgcccg aatgccgcgt
    15301 ggctgaactt tgtcgttagc tcaaaagcgc gcgccaaaat tcgtcagttg ctgaaaaacc
    15361 tcaagcgtga tgattctgta agcctgggcc gtcgtctgct caaccatgct ttgggtggta
    15421 gccgtaagct gaatgaaatc ccgcaggaaa atattcagcg cgagctggat cgcatgaagc
    15481 tggcaacgct tgacgatctg ctggcagaaa tcggacttgg taacgcaatg agcgtggtgg
    15541 tcgcgaaaaa tctgcaacat ggggacgcct ccattccacc ggcaacccaa agccacggac
    15601 atctgcccat taaaggtgcc gatggcgtac tgatcacctt tgcgaaatgc tgccgcccta
    15661 ttcctggcga cccgattatc gcccacgtca gccccggtaa aggtctggtg atccaccatg
    15721 aatcctgccg taatatccgt ggctaccaga aagagccaga gaagtttatg gctgtggaat
    15781 gggataaaga gacggcgcag gagttcatca ccgaaatcaa ggtggagatg ttcaatcatc
    15841 agggcgcgct ggcaaacctg acggcggcaa ttaacaccac gacttcgaat attcaaagtt
    15901 tgaatacgga agagaaagat ggtcgcgtct acagcgcctt tattcgtctg accgctcgtg
    15961 accgtgtgca tctggcgaat atcatgcgca aaatccgcgt gatgccagac gtgattaaag
    16021 tcacccgaaa ccgaaattaa tgttttatga acccaacacg ttatgcacgc atctgcgaaa
    16081 tgctcgccag gcggcagcct gatctgaccg tctgcatgga gcaggtccac aaacctcata
    16141 acgtttctgc gattattcgt accgcagatg ccgttggcgt acatgaagtt cacgccgtct
    16201 ggcctggtag ccgcatgcgc accatggctt cggcagcggc gggtagtaac agctgggtac
    16261 aggtgaaaac acaccgcacc attggcgatg ccgtcgctca tctcaaaggc cagggcatgc
    16321 agattctggc aacccatctt tctgataatg ctgtcgattt ccgcgaaatt gattacactc
    16381 gcccgacctg cattttgatg ggacaggaga aaacgggcat cacgcaggaa gcattggccc
    16441 tggcggatca ggacatcatc attccgatga tcggcatggt gcagtcgctg aatgtttccg
    16501 ttgcctcagc cctcattctt tacgaagccc agcgtcagcg gcaaaatgca ggcatgtacc
    16561 tgcgtgaaaa cagcatgttg ccggaagcag agcaacaacg cctgttgttt gaaggcggct
    16621 atccggtgct ggcgaaagtc gcaaaacgca aaggcctgcc ttatccccac gtcaatcagc
    16681 aaggcgagat cgaagctgat gccgactggt gggctactat gcaggctgca gggtaagtgc
    16741 catgaaaggt cgcctgttag atgctgtccc actcagttcc ctaacgggcg ttggcgcagc
    16801 acttagtaac aaactggcga aaatcaacct gcataccgtg caggatctac tcttacacct
    16861 tcccctgcgc tacgaagatc gcacccatct ctaccccatc ggagaactac tgccgggcgt
    16921 ttatgccacg gtggaaggcg aagtgctgaa ctgcaatatc tccttcggcg gtcggcggat
    16981 gatgacctgc cagatcagcg acggttccgg catcctcacc atgcgctttt tcaatttcag
    17041 cgcggcaatg aaaaatagcc tggcgacggg ccgccgtgta ctggcttatg gcgaagcaaa
    17101 gcgcggtaaa tatggtgcgg agatgatcca cccggaatac cgcgtgcagg gcgatctcag
    17161 cacgccagaa ttacaggaaa cgctcacgcc ggtttatcca acaacggaag gcgtaaagca
    17221 ggccacgctg cgtaaattaa ccgaccaggc gctggatctg ctcgacacct gcgccattga
    17281 agaactcctg ccgccggaac tgtcacaagg aatgatgacg ctaccggaag cgttgcgcac
    17341 tttgcaccgc ccgccaccga cgctacagct tagcgatctg gaaaccgggc agcatccggc
    17401 gcaacgtcgt ctgattctgg aagaactgct ggcgcacaac ctcagcatgt tagccttacg
    17461 tgccggagca cagcgttttc atgcccagcc gctgagcgcc aatgacacgc tgaaaaataa
    17521 actcctcgcc gccttaccgt tcaagccaac gggcgcacag gcacgcgtag tggcggagat
    17581 cgagcatgat atggcgctgg atgtgccgat gatgcgcctg gtacagggcg atgtaggttc
    17641 cggtaaaacg ctggtcgccg ccctcgccgc gctgcgtgcg attgcccacg gcaaacaggt
    17701 ggcactgatg gcaccaaccg aattactcgc cgagcagcac gccaataact tccgcaactg
    17761 gtttgcaccg ctcggtatcg aagtgggctg gctcgccggt aagcagaaag gcaaagcgcg
    17821 actgtctcag caggaagcca tcgccagcgg tcaggtgcag atgattgtcg gtacacacgc
    17881 catcttccag gaacaggtgc agtttaacgg cctggcgctg gtgattatcg acgaacagca
    17941 tcgttttggc gtgcatcagc gtctggcatt gtgggagaaa ggccagcagc agggcttcca
    18001 tccgcatcag ttgatcatga ccgccacgcc gatcccccgc acgctggcaa tgacggcgta
    18061 tgccgatctc gatacctcgg tgatagatga gctgccgcca ggccgcacgc cagtgactac
    18121 ggtcgctatt cctgataccc gccgtaccga catcattgac cgcgtgcgcc acgcctgcat
    18181 aactgaaggt cgtcaggcgt actgggtttg tacgttgatt gaagagtcag agttactgga
    18241 agcgcaggcg gcggaagcta cctgggaaga gttgaaactg gcgctaccag aattgaatgt
    18301 tggcctggta cacgggcgga tgaaaccagc cgagaaacag gcggtgatgg cgtcgtttaa
    18361 acaaggtgag ctacatctgc tggttgccac taccgttatt gaagtcggtg ttgatgtgcc
    18421 taacgccagc ctgatgatta tcgaaaaccc tgagcgtctg ggtctggcgc agttacacca
    18481 gctgcgcggg cgcgtaggtc gtggcgcggt ggcttctcac tgcgtgctgc tctacaaaac
    18541 gccgctttct aaaacggcgc aaattcgcct gcaagtgctg cgcgacagta acgacggttt
    18601 tgtgattgcg caaaaagatc tggagattcg cggccctggc gaattgttag gcacgcgtca
    18661 gacgggtaat gctgaattta aagtggcgga tttactgcgc gatcaggcga tgatcccgga
    18721 agttcagcgc ctggcgcgac atattcacga acgttaccca caacaggcaa aagccttgat
    18781 agaacgctgg atgccggaga cagaacgtta ctcgaatgcg taaaaggcgg cggtgagaag
    18841 accgccgctt catcggttaa ccggcaaaaa tcggcaacat taaatacagc ttaatcacca
    18901 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    18961 gcgacgggcc aaagcgttca gtgatcgcct gcatgttggc gattgccgtt ggcgttgcac
    19021 cgaggccaaa accacagtga cccgcagcca gcactgccgc atcgtagttt ttgcccatca
    19081 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    19141 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    19201 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    19261 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    19321 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    19381 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    19441 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    19501 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    19561 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    19621 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    19681 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    19741 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    19801 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    19861 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    19921 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    19981 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    20041 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    20101 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    20161 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    20221 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    20281 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    20341 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgctcaga ctcaaaacag
    20401 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    20461 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    20521 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    20581 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    20641 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    20701 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    20761 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccact
    20821 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    20881 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    20941 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    21001 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    21061 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    21121 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    21181 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    21241 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    21301 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    21361 gatccagttg actggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    21421 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    21481 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    21541 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    21601 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    21661 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    21721 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctctttc
    21781 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    21841 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    21901 agtggtgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    21961 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg atcaccgttt
    22021 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gtcagcccgc
    22081 gcccctggtg gcaaaaagtg tcgacattgc gctaagcagt cgccaactga ccgaaccacg
    22141 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    22201 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    22261 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    22321 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    22381 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    22441 gactaacctg ggtgccgaca tcgcccgcgg gacattaacc ggaaacgcgc agcgtaacgc
    22501 cgacggcagc tggcaagtgg aaaatctgcg catggcggat atccgtctac aaagcgaaaa
    22561 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgctt
    22621 ggaagtcatc gatgctcgtc tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    22681 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caggaaggca aactgtcgat
    22741 gaacgccagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    22801 attttccccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    22861 cagaacgtca gggaactggc tgcgtgacgg taaaacgttg atccttgatg atgcggcaat
    22921 tgccgggctg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    22981 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    23041 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    23101 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    23161 cgcgacattc aatcgtgttg atgttcgtcg cccgtcgctg gcgctgaccg ccaacagcag
    23221 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    23281 aagtgtttca caaacgccac aacgtcagac ccatatcagc ctgaatggac gcggtgtgcc
    23341 ggtgaatatt ttgcaacaat ggggatggcc tgaattaccg ttgactggcg acggcaatat
    23401 tcagcttacc gccagtggag atattcaggc caatgccccg ctgaaaccta cggttagcgg
    23461 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    23521 cgtttccagc ggtgaagtta catcgacgga gccggtgcgg taaacatcaa tctcgccggg
    23581 tacgactcag acgtacccgg cattccgtca atagattaca acgtaattgt cagcgcattc
    23641 ccttgaggcg tcaccaccag cccctgctca ctttcagcct gcgaaccgcc ttgcagacca
    23701 tttactttca caacattgcg caagcacagc gtccagttct tcgcctcgcc cgcaccagtc
    23761 acagtaatcg tgttgccagt acgtgctgct tttaaagtaa agatcaccga tccgtcagca
    23821 gcaggcactt cacagacggc ttcatgcccg tcttgcagat taaagaggtg gaatgccgtg
    23881 ccttcgtgcc acacgtaatc gggacgttga tcgttgttgc ccagcgccag tagagtgtta
    23941 tcacgcacat aaacgggcag actcaggaag ccgtgctgct gtttatgcca gcgactaccg
    24001 tcgagttcat cgttgtgcca caggtgtgtc cagcgacctt ccggcaggta gaactgcaca
    24061 tcgcccgctt cagtgaacac cggcgcaacc atcacgttgt cgcctaacat gtattgacgg
    24121 tcaaggtaat cacaagccgg atcgtccggg aactccatca tcatggcccg catcatcggc
    24181 gtaccccgcg cgttcgcacg cgcagcttca cgatacagat acggcatcat gcggcatttc
    24241 agttgcgtga agaagcgcac cacatcacag gactcatcat cgtaggccca cggcacacga
    24301 taagatttgc taccgtgtaa acggctatgg ctggagagca aaccaaacgc gcaccagcgt
    24361 ttgtaaacgt gcgccggagc ggtattttca aagccgccga tatcgtggct ccagaagcca
    24421 aaacctgaaa ggccaataga caaaccaccg cgcaggcttt ccgccattga ttcgtagtta
    24481 gcgtaacaat cgccacccca gtgtaccggg aatttctgcg caccgacgga ggccgagcgg
    24541 gcaaacaaga cagcttcttc ctcaccaacg gtgtccttga gcacgttcca caccagttcg
    24601 ttgtagatgt acgcataatg gttatgcatt ttctgcgaat cggaaccgtc aaaccactga
    24661 acatcaattg ggatacgttc gccaaagtcg gtcttaaagc aatcaacgcc catcgcgacc
    24721 agacctttca gtttgtcggc gtaccatttg caggcatccg gattggtaaa gtcataaatc
    24781 gccagacctg gctgccattt atcccactgc catagcgaac cgtccgggcg tttgagtaaa
    24841 tagcctttct cttgtaactc tttaaagacg ggggattttt gaccgatata ggggttaatc
    24901 cagacgcaga ttttcagccc tttcgctttc aggcggcgga tcatcccttc cgggtcaggg
    24961 aaagtcagcg ggtcccactc aaaatcgcac cactggaagg ctttcatcca gaaacagtca
    25021 aagtggaaaa catgcagcgg cagattgcgt tccgccatac catcgataaa gctgtttacc
    25081 gtcgcttcgt cgtagttggt ggtaaatgaa gtggttagcc acaggccgaa ggaccacggg
    25141 ggcggcagcg ccggacgacc agtaaagcgg gtataacgat caagtaccgc tttcggcgtc
    25201 gggccgtcga taacaaagta ttcgagatat tcactctcaa cgctgaactg cactttggag
    25261 actttctccg atcccacttc aaaagagaca cactggggat gattgaccag taccccataa
    25321 ccacggttag tcatgtagaa cgggatattt ttatacgcct gttcagtact tgtgccgccg
    25381 tcccggttcc aggtctctac cgtctggcca ttgcgcacca gggcagtaaa gcgctctccc
    25441 agaccgtaaa ctgtttcgcc aacgccaaga tcaagccgct caaacatata attgcgttga
    25501 ttattcgtgt cctgcacgta gccattattt ttcacctgac taccggtaat acgttcgccg
    25561 ttgcgcagaa aatccagtga ccagaactca cctttgctga cacgcgcgct taagttgcca
    25621 cttttaaact cagcataacg ttctgtgttt tcgattgtga ccttcacgtc ctgcaaaata
    25681 ttgagcggat aatgaggacc gttattcagc gccccctgaa aatgctcaat ccgcacaccg
    25741 acaatacctt cctgtgggga gaaaaagcgc agcgtaaata aaggcgtatc aagctgccag
    25801 gtacgttcac gcacatcacg gggggcagca tagaccacca tttcattatc ctgctgttca
    25861 acctcgaata cctgaagcgg gtgaatcaaa ttgaggccag gttgaatcaa ccagtttcca
    25921 tcgctaattt tcattgccgt tccttagttc tgcacttctt gagaggtgaa ttgttgctgg
    25981 caacggcgtt taccctgagc cagctgttcc ataacggttt tcagattgtg cgtcgtgagt
    26041 gagtagtagc gtttagcgat aatcgcgctc agcaaataac agatcgccgg aacaatcgtg
    26101 aatagcgcaa taatgatgct aatcgtggcg ctgttctgcg ctttttctgc cgcatcatat
    26161 ccgccataag ccagcatcca gccgataaga gcgccgccga aggccaaccc cagtttgagc
    26221 acaaacagcg tgccagcaaa actgatcccg gtcaggcgtt taccattgca ccactcgccg
    26281 tagtcgacgg tatcgaacat cattacccac tggataggtg tcaccagttg atgcaacaca
    26341 ccaatcacga agatgaagac aaacatagtg atgctggcct gcatgggaac aaagaacatc
    26401 gcgaggctaa tcactgccag cagggcgttc gtccaccaga agatagtgac tttacatttc
    26461 cagtcggtca gaggttttgc cagtgcggaa ccaatcaggt tacccacgca ataagtggtg
    26521 agaaaagcga caaacacttc cggcgtgccc aaaatccatg tgacgtaata catcatcgcc
    26581 ccaccgcgta cgcacaccgc caggatattg aaaatggtta gtaaaccgac aatccgccac
    26641 tggtcgtttt gccagatatc acgtaaatct tcccgcatag acgttgttgt aggtggtgct
    26701 tcaacgcgtt ctttagtggt gaagaaacaa aatgccagca tcatgaatgc caccacggaa
    26761 aggaccacga taccgccctg gaaaccgagt ggtttattat caccgccaat taaattaacc
    26821 agtggcatca tcagaacagt agaaagcatg cctcccgcgg tcgccagcac aaaacgccag
    26881 gattgcagcg agatacgctg agtcgggtca ttggtgatta caccacccaa tgcgcagtaa
    26941 gggatattga cgacggtata aagtaaggta agttagggtg taagtaattg ctgcatagat
    27001 cattttgccg ttcatactga gatctggcgt gctataggcc agtacacaga cgatcccgaa
    27061 tggcagtgcg ccaaacagta cccacggacg aaatttaccc cagcgagagc gcgttcgatc
    27121 ggccaacaac cccatgcaag gatcggaaat cgcatccagt gcacgagcga ccaaaaacat
    27181 ggttccgaca aatccggcag gaatgccaaa aatatcggta taaaagaaca tcatatataa
    27241 cattacgtta tcgaaaataa tgtggctggc ggcgtctccc atgccataac caattttctc
    27301 tttaacggac aacacttcac tcctcatctg cttttccttg atgctatagg ggtgctgaga
    27361 ccgataacgc attttgtaaa ccatccctgt gagagcgaat attccgatat ctggttatta
    27421 atttatgatt cttgttttat gtgatcgtgg tagcgttaat tccgctcata tatcattgta
    27481 aaatatgggt tttatataaa ttataatgct ttcgtgataa tacgctgcgt ttattaggcg
    27541 gaaaaaactg atctggggga tgtagaaact caaggaagta gctataatgc gccccgcctc
    27601 catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg aggcggctgg acttcaaatc
    27661 cagttggggc cgccagcggt cccgggcagg ttcgactcct gtgatcttcc gccagtttac
    27721 ctcttctgaa ctctcccaat gtcaataaat ctcttctaat cccttgtata tcagtattta
    27781 tttgtatcct gaggtcaacc tacttcaccc cgtatcaagt ttcttttggg ggtactttag
    27841 ggggtgttat ttcctattga aatacaagca cccccataat aggaaaaatg tgatacataa
    27901 caacggcttt taacaatatc tttgttatac aaggggtgta ttgggggtat caggttagtt
    27961 actggcagaa atccccctca ggaacatgaa ttcatcaaaa gccggaggat gtcatggcat
    28021 taacgatatc aaagtgaaaa ccgcaaaacc caaagacaaa ccctataagc ttgctgatgg
    28081 cggcggtatg tatctactga ttaacactaa tggttctaaa tactggcgca tgaagtaccg
    28141 ctttgctggt aaagaaaaga tgctttctat tggcgtatac cccgatgtaa cgctagccga
    28201 tgctcgcgag aaacgcagtg aagccaggaa actcctcgct gctggaggcg atccagggga
    28261 ggcaaaaaag gaagaaaaaa tcgcgcagca aatgagtctg aaaaatacgt tcgaggctat
    28321 tgctcgcgaa tggcaccagt taaaagctga tcggtggtca ttacgctatc gtgatgaaat
    28381 cattgatacc tttgaaaaag acatttttcc ctacattggc aagcgcccta ttgccgaaat
    28441 caaaccaatg aagttactgg aagcactgcg taaaatggaa aaacgtggag ctctggagaa
    28501 aatgcgcaag gtccgccagc gctgtggtga ggtgttccgc tatgcaattg ttactggccg
    28561 ggcagactat aacccagccc ccgatcttgc cagtgcttta gctacaccga aaaaagtaca
    28621 ttttcccttt cttactgcta atgaacttcc ccacttcctc aacgatctgg cgggttatac
    28681 cggaagtata atcactaaga cagctactca gatcattatg ttaaccggcg tacgaacaca
    28741 agaactgcgt tttgcacgct gggaagatat cgattttgag acaaaattgt gggagatccc
    28801 ggcagaagta atgaagatga agcgacctca tattgtgccg ctctctgagc aggtcattat
    28861 gctatttaag cagcttgaac caatctcaaa acatcatccc ctagtcttta tcggtagaaa
    28921 tgatccccgc aaaccaatta gtaaggaaag cattaaccaa gtaattgaat tacttggata
    28981 taagggaaga ctcacagggc acggtttcag acacaccatg agtacaattc tgcatgaaca
    29041 aggctttaat tccgcctgga ttgaaatgca gttagctcat gtggataaaa attccatcag
    29101 gggtacctat aatcatgccc tgtatctcga tggccgccgt gaaatgatgc aatggtacgc
    29161 tgattacatt gattcactct ccagccggga aagttaagtc agaaatactt ttccctgatg
    29221 ttttcctcaa ttaagtattt tatcctctgg cctacaaggc tggaggattt ttgttattat
    29281 attatttttt tgcgtcttca aacttattac aaaaacataa ttaaaaacaa attgacattt
    29341 ttaaattggc aattacaaaa tacagccttg cagttatttc gataccttta gcaaaggaat
    29401 agttgattca gatataattt agcatttcaa tcatctgaca tcattataaa aaatgagatt
    29461 cacataaaga taaaattatt gttctcaagg aaagcatgaa ggggcttcgt aagcaatatt
    29521 cattcggtgc caagacagga cttgatacac agaatctcaa cttttatgct ctcagactat
    29581 atcaccacat gttttacagg ctcagaaaat gaacgataga ttatgctttg aagtgcacga
    29641 taacaagggt tatttcgtat ttccggatac ctggtttggc ccattgctgg gggagtttga
    29701 agaagtgctt gatgcatacg acgctgatga aatcagcgag acgagttaca tcaacaaact
    29761 ccgacgcctt gctcaacggg agccggactt tattgatata cacgctcatc tggcatatgc
    29821 atttctggaa cagaatgccc cgcgtaaggc actgaacgcc gctcttaaag gactggcggc
    29881 aggtaatcgt atcatcccgg aaagtttctg tggtgaaata atctggatgc atcccgaaaa
    29941 ccggccttat ctgcgcgcgc tttatgcagc aattctcgca aatgtgcact tacagcgcca
    30001 tcaggatgcg gttatgctca ctgataaaat actggcatac aatcctgaag ataatcaggg
    30061 ggctcgctgg ttgttgggtt cagagctgtt acgcaccggc gatcatgagc gagccttcag
    30121 tgtactgaaa gagcatgctg atgaattttc accttactgg tatgaactcg gtctcctgca
    30181 ttttctgaac ggggaacatg ttaaggccgc cacagctttt cgccatggct ttgccacaaa
    30241 cacttacatc gcagaaatgc tgtgcggaaa tctgcatcca tttccacttg ctgtgtggca
    30301 tgacttctcc ggctctctgg atacggcaga agattactat gcaacatact caccattatg
    30361 gggacaatat ccggaagcat tgctgtttgt aaactggctc tataaccatt cctccgtgct
    30421 gcatgaacga tcagaaatca ttaaatgcgc cgaaatgctg attcaggaag atgatttcga
    30481 aatatgcgaa agtattttaa gacagcagga gcacctgtgg aaaagaattg acaagacact
    30541 ttctgaggaa attgtacaga aatgcagaaa tatgaacggt gaatatatct ggccctggat
    30601 attgcccttt tccgcagcag ggatgaaaca ttcaagcatc cagtatcagt agatattgca
    30661 ttagtgtaac tttaaaacaa gtaataattt attatccaat aataacactg tgttccttcc
    30721 ccaggtgatt accctggggt tattttctga aagtttcctt tgcaaaagga tagcgttgta
    30781 aattacttta acgaattact ccatgaactc attaaagttc tttcgtaata ctttatcgtc
    30841 aaacacgtta tagctggcat caaaaaccac agatttaccc tgccagcggg taatcccctc
    30901 ctctggcgtt ttcggttgaa ctggtatccc cgtccaacac aggcagccat tataaacagt
    30961 aaaaaattgt attcgctgtt atgaaatcaa tagtcctgac gtactgttga atgtgcttgt
    31021 gttctgaacc tcgtacaacc agtacatttg atatcgaatt ataaacaaaa cattgcatcc
    31081 aactttttta atatggacaa tagttgtcca tacggtggtt ttatattcga taaatcaatg
    31141 gatttcattg agtcacagga ctggtaatgg atgaaaatgc tttaaggttt gcctcatact
    31201 ggcgcaactc gcttgcagat gctgagtcag gaaagggctg ttttgaacgg aaagacgccc
    31261 aaaatttcac tcactggcat gggatagcgg cgggacgtct tgacgaagcg attgtcaata
    31321 aattttttaa gggagaaaaa gacgatgtcg aaactgtcaa tgtcatctta cgcccaaaag
    31381 tttatttccg gttactgcag catggtaagg accgttccgc aggcacgcct gatattgtta
    31441 caccgatagt gacgccagcc ttgctaagcc gtgagggttt tttatatccg acgccagcga
    31501 cctccattcc cagagacctg cttgaacctt tgccaaaagg agcattttcg attggtgaga
    31561 ttgggcagta tgacaaatac aagacgaccc atactgatcc tacccacgta atgtggacac
    31621 ggccctaagc gagattatgg ttttcaaatt gttccgggct gagaccgcca caggcactgt
    31681 gacgacgcca gcgattgtaa tcacactcga tataattaaa caccgttgcc cgcattattt
    31741 cccggctgct aaagcgttcc ccgtggatac attccacctt cagcgaatga aagaagcttt
    31801 ccacacaggc attgtcataa cagtagcctt tcgcactcat actgccacgc aggttgtgtc
    31861 gcttcagcat tgcctgataa tcccctgaac agtattgacc accacggtcc gtatgaacaa
    31921 tgacgctttc cgggcgtctt ctccgccaca acgccatttg cagtgcatca caggccagtt
    31981 gtgctgtcat tcgcggtgac atcgaccagc caatgacggc gcgtgaccac aggtcgatga
    32041 ctactgcgag atacaaccag ccctcatcgg tacgcaagta cgtgatgtca cccgcccact
    32101 tctggtttcg ggccactggc gctgaagtcc tgctccagca gattctccaa tacgggcagg
    32161 ccatgtgcac ggtagctgac cgggctgaac ttccggctgg ctttcgcccg cagcccctga
    32221 cgacgcaggc tggcggcaat ggttttaata ttgaactccg gcagttcgtc agcaaggcgg
    32281 ggagcaccgt atcgctgctt tgcctcaaag aatgccttat ggacagcggc atcgcaggtg
    32341 agccgaaact gttggcgcag gctcatctgg tgacgacgcc tgagccagac ataccagccg
    32401 ctgcgggcaa cccgaagtac acgacacatc gctttgatgc tgaactctgc ccgatgattt
    32461 tcgataaaga catacttcat ttcaggcgct tcgcgaagta tgtcgcggcc ttttggagga
    32521 tggacagttc ctcagcctgc tccgccagtt gtcgtttaag gcggacattt tcagcggcca
    32581 gttcgctttc gcgctctgac gaactcattt gttgctgctg tttactgcgc caggcataaa
    32641 gctgagattc atacaggctg agttcacggg ctgcggcggc cacaccgatg cgttcagcga
    32701 gtttcagggc ttcgttacga aattcaggcg tatgttgttt acggggcttc ttgctgattg
    32761 atactggttt tgtcatgagt cacctctggt tgagagttta ctcacttagt cctgtgtcca
    32821 ctattggtgg gtaagatcac cctccattaa aactaaaacg ggcgctttct gatatatcat
    32881 ctgtaagcgc agtaatacca ttagtagcac ctcgcgcatt gattattgaa tattataact
    32941 ggaatgttat atttcttgtt atgatgctgt tcagtattat aataatgtta ttatcaccaa
    33001 agataactca tgcaaatgag aaagtaatat gtcaaaacag tgcatccttt gtgaccaaag
    33061 agtttatctg cggcactata cttgtctcat taatactcag ttctctattt tgctacatat
    33121 caatatcacc atcaattttc atgcatactt atggcctgag tacttttagt tactcaatta
    33181 ttttctcctt ctcagtttta tttttcataa tcggaaatca attatcaaag attgaaaaat
    33241 tatccaacct atggttttta ttacccaata gccaatttta tcgcattaca aataaatggt
    33301 tcaaaaacag gatttgccgc atctattaca ggttttactc aaacaataag cgctggtaca
    33361 atcagtttcc tgtcagtaac cgtaagcgac aacggtgttg gtcccggaac tactttaggt
    33421 cttggttcac taataacttc attactgtca atagccgtaa tcgccattgc aggaaataat
    33481 aaaaagtaag tgtactctga ctgcgtatgt atctac
//
LOCUS       DAJVQR010000039        33136 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVQR010000039 DAJVQR010000000
VERSION     DAJVQR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(2..94)
                     /locus_tag="OQM52_001946"
     tRNA            complement(2..94)
                     /locus_tag="OQM52_001946"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OQM52_001947"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OQM52_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1563813.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OQM52_001948"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OQM52_001948"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1563814.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OQM52_001949"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OQM52_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1563815.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OQM52_001950"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OQM52_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1563816.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OQM52_001951"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OQM52_001951"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1563817.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OQM52_001952"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OQM52_001952"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1563818.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OQM52_001953"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OQM52_001953"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OQM52_001954"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OQM52_001954"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1563819.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OQM52_001955"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OQM52_001955"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1563820.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OQM52_001956"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OQM52_001956"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1563821.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OQM52_001957"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OQM52_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCS1563822.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OQM52_001958"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OQM52_001958"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1563823.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OQM52_001959"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OQM52_001959"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1563824.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OQM52_001960"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OQM52_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1563825.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OQM52_001961"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OQM52_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1563826.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OQM52_001962"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OQM52_001962"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS1563827.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OQM52_001963"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OQM52_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1563828.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OQM52_001964"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OQM52_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1563829.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OQM52_001965"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OQM52_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1563830.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OQM52_001966"
     CDS             complement(20900..21019)
                     /locus_tag="OQM52_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCS1563831.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OQM52_001967"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OQM52_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1563832.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OQM52_001968"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OQM52_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1563833.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OQM52_001969"
     CDS             complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OQM52_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1563834.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKS
                     VLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIY
                     QSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATC
                     APLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACP
                     LLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKW
                     LNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OQM52_001970"
     CDS             complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OQM52_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1563835.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OQM52_001971"
     CDS             complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OQM52_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1563836.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26945..27295
                     /gene="hypA"
                     /locus_tag="OQM52_001972"
     CDS             26945..27295
                     /gene="hypA"
                     /locus_tag="OQM52_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1563837.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27299..28171
                     /gene="hypB"
                     /locus_tag="OQM52_001973"
     CDS             27299..28171
                     /gene="hypB"
                     /locus_tag="OQM52_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1563838.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28162..28434
                     /gene="hypC"
                     /locus_tag="OQM52_001974"
     CDS             28162..28434
                     /gene="hypC"
                     /locus_tag="OQM52_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1563839.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28434..29555
                     /gene="hypD"
                     /locus_tag="OQM52_001975"
     CDS             28434..29555
                     /gene="hypD"
                     /locus_tag="OQM52_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1563840.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29552..30562
                     /gene="hypE"
                     /locus_tag="OQM52_001976"
     CDS             29552..30562
                     /gene="hypE"
                     /locus_tag="OQM52_001976"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1563841.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30636..32714
                     /gene="flhA"
                     /locus_tag="OQM52_001977"
     CDS             30636..32714
                     /gene="flhA"
                     /locus_tag="OQM52_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1563842.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32751..33104)
                     /locus_tag="OQM52_001978"
     CDS             complement(32751..33104)
                     /locus_tag="OQM52_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1563843.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7805 a         9239 c         8627 g         7465 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagcaggat aatgccgata
    24661 ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt gttccatcaa cgcatacaga
    24721 ccaccgacga acgcggtgat catgccgagc accagcagcg cgatccccca ccacagcggt
    24781 gcattaccgc ccagcagtga cagggttaac atgcccagca ggccaatttt catgactacc
    24841 gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg cacccagccg
    24901 tgcagcggaa taatcccagc cagcaggcca aagccaatca caccgagcag ccagatatcg
    24961 gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccataa
    25021 cgctgccaca gcagccagca agcaatcacc agcagcagag tgccaagacg cctcagcgca
    25081 aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacaa ggccatgact
    25141 tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac ggcggcagcc
    25201 atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg ccagtcaatg
    25261 ttatagaggc tgacaaacag accgcaaagg ccgagggtaa tcagccaaat cgcgttaagc
    25321 ggggagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc aaccgcgcca
    25381 gtcagtacag tgaagcccgc ggctgccgta tacagactac caaccgcgcc gccaattccg
    25441 gctatccagc cacttagcgc tttttgaaaa gagaaatgcc agaacagcgg cggcgacaaa
    25501 ccatgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    25561 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    25621 ctgacgcggg cgatatcggt gttatccacc agatgcaggg ctttagtcgg gcacatccgc
    25681 acacaggccg gaccttgttc atcaaagcta caaaggtcac atttgacggc gatcgcgcga
    25741 atacctggca cccagtcaag caatgtgctg acacgcgccg gagcaggcgg tgccggtggc
    25801 gctttcgggg tattggcgtt tgccggaata tccagcggac ggctgccgga aaattcaatt
    25861 gcgccaaacg ggcaggcgat gccgcacagt ttgcagctta cgcacagact ttcattcaac
    25921 tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca
    25981 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattgagcat cactctcagg
    26041 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaggtg
    26101 tggcagccga tacagagcgt ggagtcggca attacaaaac gattcaccag gcattcctca
    26161 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    26221 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    26281 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatggcggt
    26341 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    26401 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    26461 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    26521 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    26581 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    26641 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    26701 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttactcgttt aacagagatg
    26761 ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga gatttttaag
    26821 ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    26881 cgcagggata atcaacactg gcgcaattat tgcttgtagc tggcaatagt taatgggagg
    26941 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    27001 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    27061 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    27121 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    27181 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    27241 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    27301 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    27361 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgcaga tgaaaatcac
    27421 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    27481 tcatggcgaa gcgggcactc acacaccggg catgagccag cgtcggatgc tggaagtcga
    27541 aattgacgtg ctggacaaaa acaaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    27601 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    27661 gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc gcggttattg aaggcgacca
    27721 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggt acaccagcga ttcaggtgaa
    27781 caccggtaaa ggctgccatc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    27841 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    27901 cttcgatctc ggtgaaaaac acaaagtagc ggtgctttcc gttaccgaag gtgaagacaa
    27961 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    28021 cctgttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa
    28081 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    28141 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    28201 ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    28261 ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt tcacgttggc
    28321 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    28381 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    28441 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    28501 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgtggcg
    28561 gtcataccca cgccattttt aaattcggcc tcgaccagtt actgcctgaa aacgttgagt
    28621 ttatccacgg tccgggttgc ccggtgtgcg tactgccgat gggtagaatc gacacctgcg
    28681 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac
    28741 cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    28801 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgcaaa gtggtgttct
    28861 tcggcttagg ttttgaaacc accatgccga ccaccgccat cactctgcaa caggcgaaag
    28921 cgcgtgatgt gcagaatttt tacttcttct gccagcacat tacgcttatc ccgacgctgc
    28981 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    29041 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    29101 tggtcgctgg tttcgaaccc cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga
    29161 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg ggtggtaccg gatgccggta
    29221 acctactggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac agcgaatggc
    29281 gcggcttagg cgtgattgaa tcttctggcg tgcacctgac gccggattat caacgattcg
    29341 atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg cgcgcgcgtt
    29401 gtggtgaggt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    29461 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    29521 ggtatcagta tcgtcagcag gagagtgaag cgtgaataat atccaactcg cccacggcag
    29581 cggcggccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    29641 gtggctggca gagcaggaag atcaggcacg tcttgatctg gcgcagctgg tagcggaagg
    29701 cgaccgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    29761 taatatcggc aagctggcga tttgcggcac cgcgaatgac gttgcgggca gtggcgctat
    29821 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    29881 agccgtagtg accagcatgg cagaaaccgc ccgcacggca ggcattgcca tcgttactgg
    29941 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    30001 gggcgcaatt ccggcgaata ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt
    30061 gctggttagc ggtacactcg gcgaccacgg ggcgactatc cttaacctgc gtgagcagct
    30121 ggggctggat ggcgaactgg tcagcgactg cgcggtgctg acgccgctta ttcagacgct
    30181 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt
    30241 ggttcatgag ttcgcggcag cctgcggttg tggtattgaa atttctgaat cagcgctgcc
    30301 ggttaaacct gccgtgcgcg gcgtttgcga attgctggga ctggacgccc tgaactttgc
    30361 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggcagc
    30421 gttacattcc catccactgg ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa
    30481 aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc ctcgatttac cacacgccga
    30541 accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    30601 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    30661 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    30721 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    30781 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcgtcgcgt gaaaaagaca
    30841 cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc agcattttgt
    30901 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    30961 ccgcaggtgg gctatacccc aaatttggtc actattgcct gatgccactg gcggcgggag
    31021 ggcatatttt tggtggctgt gaatttattc gttatgacga gcgtccctgg agcgaaaaag
    31081 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    31141 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    31201 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    31261 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagcatt gtcttacgca
    31321 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    31381 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    31441 aagagatgct gctgatcaat ctctacgagc gggacgattt agccccctat gaacgcatgt
    31501 tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    31561 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    31621 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    31681 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    31741 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    31801 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    31861 gtgaaactgg ctcgggtaaa gagctgattg cccgtgcgat ccataatctc agtgggcgta
    31921 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg
    31981 atctgtttgg tcatgagcgt ggggctttta ccggtgccaa cgcccagcgc attggtcgtt
    32041 ttgaactggc ggataaaagc tctctgttcc tcgacgaagt gggcgatatg ccactggagt
    32101 tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca
    32161 aaatcattca gacggacgta cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg
    32221 tcgccgaccg agagttccgt agcgatctct attaccgcct gaacgtattc ccgattcacc
    32281 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    32341 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ctgcgcacct
    32401 tgagcaacat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcag
    32461 tattgctaac acgcggcaac gtgctgcagc tgtcattgcc agatattgct ttaccggagc
    32521 ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga
    32581 ttgtgcgcgt gctgaaagaa accaacagcg tggttgccgg gcctaaaggc gctgcgcaac
    32641 gtctggggct gaaacgcacg accctgctgt cacggatgaa gcggctggga attgataaat
    32701 cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga
    32761 ggttttttcg gacgatattt ttccggcagt tctggcaccg gacgcttgtc atcgatgaga
    32821 tgacgcacgg ttaagatcgg atgacgccac agcattctcg gcccggccca acgcataatc
    32881 tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg cttacaggct
    32941 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    33001 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    33061 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc tccacctcat
    33121 taagatgcat ttatat
//
LOCUS       DAJVQR010000040        30867 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVQR010000040 DAJVQR010000000
VERSION     DAJVQR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            69..1727
                     /locus_tag="OQM52_001979"
     CDS             69..1727
                     /locus_tag="OQM52_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1563844.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1724..2680)
                     /locus_tag="OQM52_001980"
     CDS             complement(1724..2680)
                     /locus_tag="OQM52_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS1563845.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2831..3946
                     /gene="macA"
                     /locus_tag="OQM52_001981"
     CDS             2831..3946
                     /gene="macA"
                     /locus_tag="OQM52_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1563846.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3943..5889
                     /gene="macB"
                     /locus_tag="OQM52_001982"
     CDS             3943..5889
                     /gene="macB"
                     /locus_tag="OQM52_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1563847.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5962..6186)
                     /gene="cspD"
                     /locus_tag="OQM52_001983"
     CDS             complement(5962..6186)
                     /gene="cspD"
                     /locus_tag="OQM52_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1563848.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6509..6829
                     /gene="clpS"
                     /locus_tag="OQM52_001984"
     CDS             6509..6829
                     /gene="clpS"
                     /locus_tag="OQM52_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1563849.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6860..9136
                     /gene="clpA"
                     /locus_tag="OQM52_001985"
     CDS             6860..9136
                     /gene="clpA"
                     /locus_tag="OQM52_001985"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1563850.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9539..9626)
                     /locus_tag="OQM52_001986"
     tRNA            complement(9539..9626)
                     /locus_tag="OQM52_001986"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9590..9592),aa:Ser,seq:gga)
     gene            complement(9880..10098)
                     /gene="infA"
                     /locus_tag="OQM52_001987"
     CDS             complement(9880..10098)
                     /gene="infA"
                     /locus_tag="OQM52_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1563851.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10383..11087)
                     /gene="aat"
                     /locus_tag="OQM52_001988"
     CDS             complement(10383..11087)
                     /gene="aat"
                     /locus_tag="OQM52_001988"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1563852.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11129..12850)
                     /gene="cydC"
                     /locus_tag="OQM52_001989"
     CDS             complement(11129..12850)
                     /gene="cydC"
                     /locus_tag="OQM52_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1563853.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12851..14617)
                     /gene="cydD"
                     /locus_tag="OQM52_001990"
     CDS             complement(12851..14617)
                     /gene="cydD"
                     /locus_tag="OQM52_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1563854.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14740..15705)
                     /gene="trxB"
                     /locus_tag="OQM52_001991"
     CDS             complement(14740..15705)
                     /gene="trxB"
                     /locus_tag="OQM52_001991"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1563855.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16249..16743
                     /gene="lrp"
                     /locus_tag="OQM52_001992"
     CDS             16249..16743
                     /gene="lrp"
                     /locus_tag="OQM52_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1563856.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16878..20906
                     /gene="ftsK"
                     /locus_tag="OQM52_001993"
     CDS             16878..20906
                     /gene="ftsK"
                     /locus_tag="OQM52_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1563857.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDAPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21061..21672
                     /gene="lolA"
                     /locus_tag="OQM52_001994"
     CDS             21061..21672
                     /gene="lolA"
                     /locus_tag="OQM52_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1563858.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21683..23026
                     /gene="rarA"
                     /locus_tag="OQM52_001995"
                     /gene_synonym="mgsA"
     CDS             21683..23026
                     /gene="rarA"
                     /locus_tag="OQM52_001995"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1563859.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23117..24409
                     /gene="serS"
                     /locus_tag="OQM52_001996"
     CDS             23117..24409
                     /gene="serS"
                     /locus_tag="OQM52_001996"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1563860.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24649..27092
                     /gene="dmsA"
                     /locus_tag="OQM52_001997"
                     /pseudo
     CDS             24649..27092
                     /gene="dmsA"
                     /locus_tag="OQM52_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27103..27720
                     /gene="dmsB"
                     /locus_tag="OQM52_001998"
     CDS             27103..27720
                     /gene="dmsB"
                     /locus_tag="OQM52_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1563861.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLLRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27722..28513
                     /locus_tag="OQM52_001999"
     CDS             27722..28513
                     /locus_tag="OQM52_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1563862.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28549..29175)
                     /locus_tag="OQM52_002000"
     CDS             complement(28549..29175)
                     /locus_tag="OQM52_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1563863.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29490..30638
                     /locus_tag="OQM52_002001"
     CDS             29490..30638
                     /locus_tag="OQM52_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1563864.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7590 a         7596 c         8306 g         7375 t
ORIGIN      
        1 cgtattagca aggcctttcc cgttatactg cgagcgtaaa ggataagtca catatttctg
       61 gaggggatat gattcttgag cgcgttgaaa ttgtgggttt tcgcggtatc aaccgtttgt
      121 cgttgatgct cgaacaaaac aacgtcctga ttggggagaa cgcgtggggt aaatccagct
      181 tgctggacgc cttaaccctg ctgctatcgc cagaatcaga tctctaccat tttgagcgcg
      241 acgatttctg gttcccgccg ggagatatca acgggcgaga acatcatctg catattattt
      301 tgaccttccg cgaatcgctg ccaggccgac atcgggttcg ccgttatcgg ccgctggaag
      361 cgtgctggac gccatgcacc gatggctatc accgtatttt ttatcgtctg gaaggggaga
      421 gtgcggaaga cggcagcgtg atgacactgc gcagttttct cgataaagac ggacatccga
      481 ttgatgttga ggatattaac gatcaggcac gccatctggt gcgtttaatg ccggtgctgc
      541 gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg cacggtgcca aatgtcccca
      601 atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg tgagttgtcc tcacatccgc
      661 aaaatctctc tgatggacag attcgtcagg gactttccgc aatggtgcag ttgcttgagc
      721 attatttctc tgagcagggg gctggacagg cgcgatatcg tttaatgcgg cggcgggcca
      781 gcaatgagca acgaagctgg cgctatctgg acatcatcaa ccggatgatt gaccgacctg
      841 gagggcgctc gtatcgggtt attttgctcg gcctgtttgc tactttattg caggcaaaag
      901 gcacattgcg actggataaa gacgcgcgtc cgttattgct aattgaagat ccagaaaccc
      961 gcctgcatcc gattatgctt tccgttgcct ggcatctgtt gaatcttttg cctttacagc
     1021 gtattgctac caccaactct ggcgagctgc tttcgttaac tccagtggaa catgtttgcc
     1081 gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct ggggccaagt ggcttgagca
     1141 ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa tcgtccgtca tcgctgtttg
     1201 ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg ggttatcaat gaactggcgc
     1261 gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt cattgagttt gcccagtccg
     1321 ggctaaagcc actggttaaa tttgcccgcc gaatggggat tgaatggcat gtactggtcg
     1381 atggcgatga agcagggaag aaatatgccg ctacggtacg cagcctgttg aataacgatc
     1441 gggaagccga acgagaacat ttaacggcgt taccggcgct ggatatggaa cattttatgt
     1501 atcgccaggg attttccgat gtgttccacc gcgtggcgca aatcccggaa aatgtaccga
     1561 tgaatctacg caaaattatc tcgaaagcga tccatcgctc ttccaaaccc gatcttgcca
     1621 ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc cgtaccaacg ctgctgaaaa
     1681 aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc ggattaaccg cgaaacatcg
     1741 tggccatttg tggctgaata gcgtcgagca tctcatagcg ccgacggtat tcagcccgtt
     1801 ttttactggc gatttcggca atctcttttc gtgctatctg tgctggaagg cggtaatggc
     1861 gttcagcatc acatacgccg ccaaccgact cccagaacgc gttgtaatca gcatggatct
     1921 tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg cgtttcattg ctgacggcaa
     1981 taatctgctc tacctgcaaa cgttgggcaa acagacaggc cgcttccatc acgaggcgtt
     2041 tgggaaatag cccgtggcag gctttcgtcg cattctggat ttcctgatgt ggaatttccc
     2101 attttgcgcc ttgcagtccg ccaataaaca tcgttctttt cccctgatat tcacacaggg
     2161 taaacgtgat ctctgccaga ggaatacctt cgctgttgcg gaacaggatt gtgctgtcac
     2221 cttctttatc cattgagatc atcatggtca gctcaagcgt gaactgctca ccgtttttgc
     2281 cttccagttt agccagttgc agcccggggg tattcaaata taagctgaat tcttccgccg
     2341 acatacaccc gcggagtaac gcataatggt aacgtaacgc ctccagcaat tgcttacggc
     2401 taagattcgc ggcaaggtaa gggcgatgca gacgcacagg cagtcgcggc tggcgcgtta
     2461 acaacacatt gagattaggc cagtgggaaa gttcgttcat ccactcaacg cttaaacgcg
     2521 gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact acagcgatgc cagaatttac
     2581 ccggccgaca ctgtccacgt gccagactaa gaaaaagtga caggctgctg agagattcag
     2641 atggcgtaaa ggtccgttca gttagctgcg acatattcat gaaatcaatg gttatacatg
     2701 acgtcgattt caccattgcg tatcttaacc aaacatcaat agtgtgatta ctaacgtaaa
     2761 ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc ggattggcat atggagtatt
     2821 cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt tacgttattg cgctggtgat
     2881 agtcatcgcc ggactgatta cgttatggag aattcttaac gcacccgtgc cgacttatca
     2941 gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg ctggcgaccg gaaagctgga
     3001 cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt cagttgaaaa cgctgtcggt
     3061 ggcgattggc gataaagtaa aaaaagacca gcttttaggg gtcattgatc ctgaacaggc
     3121 tgaaaaccag atcaaggagg tcgaagcaac gctgatggag ctacgcgcgc agcggcagca
     3181 ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc cgtcagcaac gtctggcaca
     3241 aacgcaagcg gtttcactgc aggatctcga taccgccgcg acggagatgg ctgtgaaaca
     3301 ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat caggcctctc tcgatacggc
     3361 taaaaccaat ctcgattaca cccgtatcgt tgccccgatg gctggggaag tgacgcaaat
     3421 caccactctg caaggccaga cggtgattgc cgcacagcaa gcaccgaaca ttctgacgct
     3481 ggcagatatg agcactatgc tggtaaaagc gcaggtttct gaagcggatg taatccacct
     3541 gaagccgggg caaaaagcct ggtttacggt gcttggcgat ccactgacgc gctacgaggg
     3601 gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac gacgctattt tctattacgc
     3661 ccgttttgaa gtacccaacc ccaatggttt gctgcggctg gatatgaccg cgcaagtgca
     3721 tattcagctc accgatgtga aaaatgtgtt gacgatccct ctgtcggcgt taggcgatcc
     3781 ggttggcgat aatcgttata aagtcaaatt gttgcgtaat ggtgaaacac gcgagcgtga
     3841 agtgacgatt ggcgcacgta acgataccga tgttgagatt gtcaaagggc tggaagcggg
     3901 cgatgaagtg gtgattggtg aggccaaacc aggagctgca caatgacgcc tttgctcgaa
     3961 ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc aggttgaggt gctgaagggc
     4021 atcagcctca atatttatgc gggtgagatg gtcgcgattg ttggcgcttc gggttccggt
     4081 aaatcgaccc tgatgaatat tctcggctgc ctggataagg ccaccagcgg cacctatcgc
     4141 gtagccggtc aggatattgc cacgctggac gccgatgcgc tggcgcaact gcgccgcgag
     4201 catttcggct ttattttcca gcgttaccat ttgcttccgc atttaaccgt cgagcagaac
     4261 gttgaagtac ccgccgtcta tgccggtctt gagcggaaac agcgactgct tcgtgcccag
     4321 gagttgctgc aacggctggg gctggaagac cgtacagagt attatccggc acagctttcg
     4381 ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga tgaacggcgg tcaggtaatt
     4441 cttgccgatg aaccgaccgg cgcactggac agccattctg gcgaagaggt gatggcgatc
     4501 ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg tcacccacga tccgcaggtc
     4561 gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg aaattgtgcg caatcctccc
     4621 gccattgaaa aagtgaatgt tgctggcggg acggagcctg ttgtcaatac agtgtctggc
     4681 tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga tggcatggcg ggcgctggca
     4741 gcgaataaaa tgcgtacttt actgaccatg ctggggatta ttatcggtat tgcgtcggtg
     4801 gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg tgctggcgga tattcgttct
     4861 attggtacga atactattga tgtctatccc gggaaagatt ttggcgatga cgatccgcaa
     4921 tatcagcagg cgctgaagta cgacgactta atcgccatcc aaaaacaacc gtgggtcgcc
     4981 tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt ataacaatgt tgatgttgct
     5041 gccagtgcca atggcgtgag cggcgattat tttaatgtct atggcatgac cttcagtgaa
     5101 ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc aggtcgtggt tctcgacagt
     5161 aatactcgcc gccagctttt cccccataaa gcagatgtgg ttggcgaggt gattctggtc
     5221 ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa aacagtcgat gtttggtagc
     5281 agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt ccgggcgagt tatgggccag
     5341 tcgtggctta actccattac tgtcagggtg aaagaaggat ttgacagcgc cgaggcggaa
     5401 cagcaactca cgcgtttact ttcactgcgc cacggtaaga aggatttctt tacctggaac
     5461 atggacggcg tcttgaaaac tgttgaaaag accacacgta ctttacaact gtttctgacg
     5521 ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg taatgaatat tatgctggtg
     5581 tcagtgaccg agcggacgcg ggaaattggc attcgcatgg ctgtaggtgc gcgagcaagc
     5641 gatgttttgc aacagttcct gatcgaagcc gtactggttt gcctggtcgg tggcgcgttg
     5701 ggaataacac tgtcactgtt aattgctttc accttgcagc ttttcttacc cggctgggag
     5761 attggttttt caccgttggc gctgctgctg gcgtttctct gctcgacggc caccgggatt
     5821 ttatttggct ggttacccgc acgaaatgcg gcacgactgg atccagtaga tgctctggca
     5881 cgggagtaat ttttgagata aaaatgccag ccgatcgggc tggcattttg cctttaggat
     5941 gtacacaatg agacagaaga gctatgcgac tgccgcttct acttcgacgg gcacaataac
     6001 actggcgtga ttgccttttg gcccctggtg gacatcaaac tgaacggatt gtccagcttt
     6061 tagcgttctg taaccatcca tctgaatggt ggaataatga gcgaaaatat cttcgccgcc
     6121 gccttcaggg cagatgaaac caaacccttt ggcattgttg aaccacttaa cagtaccctt
     6181 ttccatgctt cgacatcctt cgcaaatctt atacaagtaa gatggaataa accggggtca
     6241 gagagggggc tgttcaaaac ctcgccaact ctagaaatac aatttagaga attagggcga
     6301 gccgtcaagc atttgacagg ggccaggggg caggtatgaa tcaaaaattt gaagcagtta
     6361 acgctattga caggaatgtg acagatgtcg ctgatgccaa cgatagatga tagttatcta
     6421 tcatgtggag tagattggtc aggcaaataa gctcttgtca gcggcagggc gttctgccga
     6481 taaccgtaac cgaagatgat aactgacaat gggtaaaacg aacgactggc tggactttga
     6541 tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg ccatctatgt ataaagtgat
     6601 attagtcaat gatgattaca ctccgatgga gtttgttatt gacgtgttac aaaaattctt
     6661 ttcttatgat gtagaacgtg caacgcaatt gatgctcgct gttcactacc aggggaaggc
     6721 catttgcgga gtctttaccg ccgaggttgc agaaaccaaa gtggcgatgg tgaacaagta
     6781 cgcgagggag aatgagcatc cattgctgtg tacgctagaa aaagcctgaa tgcaggcata
     6841 aaaattgggg gaggtgccta tgctcaatca agaactggaa ctcagtttaa atatggcttt
     6901 cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc gagcacttgt tactggcgct
     6961 gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt tctgtggatt tggttgcgct
     7021 ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc gttctgcctg ccagtgaaga
     7081 ggagcgcgac acacagccga cgctgagttt tcagcgtgta ctgcaacgtg cggtcttcca
     7141 tgtccagtcc tccggtcgca atgaggttac cggtgcaaac gttctggtcg ctatcttcag
     7201 cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat gaagtcagcc gtctcgatgt
     7261 ggtgaacttt atctctcatg gcacgcgtaa agacgagccg acacagtctt ctgatcctgg
     7321 cagccagcca aacagcgaag aacaagctgg tggggaggaa cgtatggaga atttcacgac
     7381 gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca ctaattggtc gtgagaaaga
     7441 gctggagcgt gctattcagg ttctctgccg tcgccgtaaa aacaacccgc tgctggtggg
     7501 ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt gcctggcgaa ttgttcaggg
     7561 tgatgtgccg gaagtgatgg ctgactgtac gatttactct ctcgatatcg gttctctgtt
     7621 agcgggcaca aaatatcgcg gcgactttga aaaacgtttt aaagcgttgc tcaagcagct
     7681 ggagcaggac actaacagca tcctgtttat tgatgagatc cacaccatta tcggtgcggg
     7741 tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc aaaccgttgc tctccagcgg
     7801 taaaattcgc gtaattggtt cgacaaccta tcaggagttc agcaacattt tcgagaaaga
     7861 ccgtgctctg gcgcgtcgct tccagaaaat tgatattact gaaccgtcga tcgaagaaac
     7921 tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg caccacgacg tgcgttatac
     7981 cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa tacattaacg atcgtcatct
     8041 gccggataaa gccattgatg ttatcgacga agcaggcgct cgcgcacgtc tgatgccggt
     8101 aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag tccgtggtgg cccgtattgc
     8161 acgcattcca gagaagagtg tttctcagag tgaccgcgat accctgaaaa aactcagcga
     8221 tcgcctgaaa atgctggtct tcggccagga taaagccatt gaggcgctga ctgaagccat
     8281 taagatggcg cgtgcaggtt taggtcacga acataaaccg gtcggttcgt tcctgtttgc
     8341 cggtcctacc ggggtcggga aaacagaggt gacggtacag ctttcgaaag cgttgggcat
     8401 tgagcttctg cgctttgata tgtccgagta tatggaacgc cataccgtca gccgtctgat
     8461 tggtgcgcct ccgggatacg ttggttttga tcagggcggt ttgctgaccg atgcggtaat
     8521 caagcatcca catgcggtgc tgctgctgga cgaaatcgag aaagcgcatc cggacgtgtt
     8581 caatattctg ttgcaggtga tggacaacgg tacgctgacc gataacaacg gacgcaaagc
     8641 ggacttccgt aacgtggtgc tggtgatgac cactaacgcc ggggtacggg aaactgagcg
     8701 caaatccatt ggtcttatcc accaggataa cagcaccgat gcgatggaag agatcaagaa
     8761 gatctttaca ccggaattcc gtaaccgtct cgacaacatt atctggttcg atcatctgtc
     8821 aactgacgtg atccatcagg tggtggataa attcatcgtc gagttgcagg ttcagctgga
     8881 tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt aactggctgg ccgagaaagg
     8941 ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc atccaggaca acctgaaaaa
     9001 accgctcgcc aacgaactgc tgtttggttc gctggtggac ggcggtcagg tgacggttgc
     9061 gctggataaa gagaaaaatg agctgactta cggattccag agtgcacaaa agcacaaggc
     9121 ggaagcagcg cattaatctg attgtcaggt aggttggtga agtccgtaat ctcgtcaggg
     9181 gttacggact ttttatttat ggggtggaac ggtgaaaacc ctatttttgg aggtgaaggt
     9241 aagttgttga taattagtgc tgctggaagg taaggataaa aaagggtgct gcaggagaat
     9301 gggatggttt tgctttatta acaacgggct aaacgtgtag tatttgagtt cactgccgta
     9361 caggcagctt agaaatcgac ggggtgcggt aaaacctttg cgaacgcgtt cactgccgta
     9421 caggcagctt agaaattcac aggtaacata ctccacccgc ccaccatgtt cactgccgta
     9481 cagacagata aaatgcgaaa aaaaagctcg cactttcgta cgagctcttc tttaaatatg
     9541 gcggtgaggg ggggattcga acccccgata cgttgccgta tacacacttt ccaggcgtgc
     9601 tccttcagcc actcggacac ctcaccaaat tgttttgcta ccaaacctca tgggtggcaa
     9661 cggggcgcta ctatagggag ttggagtaaa acggtcaaga agaattttaa tgataattat
     9721 tgtttgctca tactgtaaac aacttgtgca gtatatctac atcgagacaa gttatggact
     9781 tatacttcca aagtacttca tacatatcac aaaataaaaa ggccggttaa accgaccttt
     9841 tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac ggaagacaat
     9901 gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc ccgtcaggat
     9961 gcggatgtag tttttgcgca ttttaccgga gatgtgtgca gtaaccacgt gaccgttttc
    10021 taactctacg cggaacatgg tattaggcaa cgtttcaaga acggtacctt gcatttcaat
    10081 attgtcttct ttggccatct aatcctctgg ggtatcacta ccgtaatttg aaccggcaag
    10141 ataatgccga agttctgtaa ataagtaaag atttgcgcgc taaatcgcaa caaacaggtt
    10201 cggcacatta ctccgaaaac acacggctaa gccgcaccaa aagcgcaacg tataagggag
    10261 cggtgagata aacgatgggc gttacctgac gcgaaaaatt ccttatcggc agcggggtaa
    10321 tgagcgtaac caactctgcg accgcaatta taacactctg gggagaaatg tgccgaaaac
    10381 attcattctt gtggtgaaaa caagcaccgt ggtacccaga aattattcgg caatcgtccg
    10441 aggcgcattt gattgagata attaaggtaa tcccggcggg gaatttcgca ggcaccaagc
    10501 gatgctgtgt gatcgttaag gacctggcag tcgataagct taccgccatg accgataaat
    10561 tcctcacaga ataccagaag cgccgtttta gacgcatttt ccatccggct gaacatggac
    10621 tcgccacaaa atagcgttcc ctgggccacg ccgtacatac cgccgacaag ctcatcttca
    10681 cgccagactt caatggagtg ggcatgaccg agttcgtgaa ggcgatggta ggcttcgacc
    10741 acgccacgcg tgatccaggt tccttcttcg cgatcgctgg cacagccttc aatgacctga
    10801 ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt tatgaaatcg cttcatacta
    10861 cggctgatat gcagtgattc tggccatagc accgcgcggg gatcgggcga ccaccagagg
    10921 atggggtcgc ctggagaaaa ccacggaaaa ataccacgct ggtaagccat taacaggcgc
    10981 gcagggctaa gatcgccccc aagtgccagc aggccgttag gctcacgtaa tgcgccttcc
    11041 ggggaaggga aggctattga atggcgagaa agctgaacca agcgcatgac cgcaaaactc
    11101 cacgcaagtc ggatcgttca ataatagctt acaaaccctg cttgaactgg taataacgcc
    11161 cctgtctggc aagcagttct gcgtgagtac cttgctcaat aatttgcccg ttgtccatca
    11221 ctattatttg ttggaaacga gagagtccgc gaagtcgatg ggtgaccatt aacaccgttt
    11281 tctcacgcat catttttgca agcaattcaa ggatctggct ttcggttgtg gcatctaagc
    11341 cttcggtagg ttcatccagc aacaccagtg gcgcatcatg taacagcgta cgggcgatag
    11401 ccagacggcg cagttcacca ccggagagct ggcgtccgcc ttcacctaac caactgttga
    11461 gacctgcatc ctcgagcagc ttctccagac caacgcgacg caagatctcc gacagagcct
    11521 catcactact gccaggcgag gcgagtaaca gattatcacg cagtgtggcg ctaaacagat
    11581 gcactcgctg aggaacaacg ctgatggtct gtcgtagagc cgcttcattc aggctggcta
    11641 tggggctatc gttaagcaaa atctcgccct gttgcgggtc ccatgcgcgg gtcagcagtt
    11701 gtaacagtgt tgatttgccg catccggttc gcccgagaat cgctatatgt tccccggcgt
    11761 ttacctgaag agaaatccct ttaagtgcct gttgggattg ctccggataa gtgaactgaa
    11821 catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt ttgggtatca gggaaggtga
    11881 cctccggttt ttgatccgtt aagtcagaga tacgtacggc agaggcaatg acttgcccca
    11941 gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa cgcggctaac gcacaaaaga
    12001 caaacagggc aattaatgcg ccgggttgag cattgccgcc aacgccgcca gacgccatcc
    12061 acagcatcag gatcaccgct aacgcgccaa tgagcagcat tatcgcttgc gacaatgcgg
    12121 tcagttcaga ttgacggcgt tgcgcttcca gccattgaat ttctgtattc tctagttgcg
    12181 tgcgataacg atcgctggca ccaaaaatgg tcagctcagc ttgcccttgc agccaggccg
    12241 tcagttgttg gcgatactgt ccgcgaagat gagtcagatt ttgcccggtg ctttttcccg
    12301 cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa cataacgctg cccagcgtaa
    12361 aggcgagggt gaaatcaagg aaacttaacc cgattgtcac caccataatc accacaaaag
    12421 cgcccaccag cggcgagata acgcgcaggt aaagatgatc gagcgtatca acatccgcca
    12481 ccacgcggtt gagcaattcg ccctgacgat agcgcgccag tccggcaggg gagaggggca
    12541 acaatttgct gaaggtgtaa atgcgcagat gctgcaacac gcggaaagtc gcgtcgtgac
    12601 ttaccagacg ttcaaaataa cgcccggcag tacgggtgat tgcggcacca cgcacgcccg
    12661 cagcgggtag catatagttg aagctgtaca gtccggcaac ccccgcaacc gctgaggccg
    12721 agaggaacca gccggaaagt gtcaacagac cgatactggc gagcagcgtc acaattgcca
    12781 gcacaatacc aagacttaac atccatttat gacgtttata cagtgccaga tagggtagca
    12841 aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa tgtggcgaat gggccaccag
    12901 ccacacttaa ttccgcgtaa cgtccttgct caataatccg gccatcctgc ataacccaaa
    12961 tgacatccca gtcagcaaga tcttctaact ggtgggtgac cattaacgtt gtctggcgca
    13021 gagaggcggc attcagcgcc tccattacgc gctgttcact gtgagcatca aggctggcag
    13081 cgggttcatc caacagtaat agcgaacagg gatttagtaa cgcacgggcc accgccacgc
    13141 gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc aacaggtgta tcaacgcctt
    13201 gtggcaggag cggtagaaac tcgctgaccc aggcgttatc cagcgctgct tgtaattctt
    13261 gttcgctggc atcaggtcgc gccagtagta cgttatcccg caattttgct gccggtaatt
    13321 gtgggttttg cccaacccag gagagatgtt tacgccatga ttctggtgat aaatcacgta
    13381 attctatccc gttgattcgt aatgatccct gatatgagag aaaaccagaa agcgcgttca
    13441 gcagtgagct tttacctgaa ccgctgcggc caaccaacac tgcacgttgg cctgctggca
    13501 aagtaaagtt cagcggtccg gccagcgttt taccttccgg cgacgtgata aacagatcct
    13561 cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc ctcaccgcgc tgcggatggg
    13621 cgagcggggt ttccataaac gttttcagac tgtcagctgc accaacagcc tgggctttag
    13681 catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa ctctggcgca aggatcaggg
    13741 ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg accaaaatcc agctcgccga
    13801 gataggaaaa accaaagtag accgccacca gagcaattga cagcgaggta aaaaattcga
    13861 gaatgccgga ggataaaaac gccagtcgta gcacttccat tgtccgttgg cggaaatctt
    13921 ccgaagcaga acgaatactt tcaatttcag cttcaccacg accaaaaata cgcaatgttt
    13981 ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg agcaagagcg agaaagttac
    14041 gccggttagc atcggcagcc cccattccaa ccagcgccat aaacaacgga attagcggtg
    14101 ctgtgcccag cagaatgagc gccgcagccc agttagaggg gaagatcgcc accacaatca
    14161 gcaacggcac cgacactgcc agcgccattt gcggcagata gcgtgcatag taatcatgca
    14221 tatcgtcaat ttgctcgagt accagcgtcg cccagctccc cgcaggttta ccctgaatcc
    14281 acgctggccc tgcttgttgc agacggtcga gaacctgacg gcggatggca aagcggatat
    14341 gctgcccggc gtgataaccc acccgttcgc gtaaccagac cacccatgcg cgcaatacaa
    14401 aggtcagaac cagtaacgta aagggaagca gcagggcttc acggggaata ttctccataa
    14461 tcatatgttg cagaattcgc gccatgaacc aggcctgggc aatgatcaat atgccgctta
    14521 caaagcccag cagacgagaa atattcagcc aacgttggga gatgacgctt tgctgtttta
    14581 accagcgggt taactctttt tgacgagatt tattcattgc acgcttagca ggtgagttat
    14641 cagaattatt tgcagagcaa tgttacaacg gggaaaaaat aaaggcgacc catagtcgca
    14701 aggtgtcgcc ttctttactt ttgttactga tttgtaaaat tattttgcgt cagctaaacc
    14761 atcgaggtag cgttccgcat caagtgctgc catgcagcca gtaccggccg aagtaatggc
    14821 ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag acgccaggaa tgctggtctg
    14881 ggtggcatta ccatgaatac ccgactgtac tttgatgtag ccgttttcca gttccagctg
    14941 cccttcgaaa atcgcagtat tcgggctgtg accgatagca acaaacagac cggcaacgtc
    15001 gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga cgaacgccag tgacacccat
    15061 ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc agaatgatgt tgccgttctc
    15121 cactttatcc atcagacgct taataaggat tttttccgcg cggaaaccgt cacggcggtg
    15181 aatcagatgc acttccgaag cgatgttaga cagatacagc gcctcttcaa ccgcggtatt
    15241 gccgccgccg atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca
    15301 agcagaaacc ccacggcctt taaaggcttc ttcagagggc aggccgagat agcgtgcaga
    15361 agctccggtg gcaataatca gcgcgtcgca agtgtattcg ccgttatcgc cattcagacg
    15421 gaacggacgg ttttgcagat ccaccttgtt gatatgatca aaaatgatct cagtttcaaa
    15481 cttggtggca tgttcgtgca tgcgcgccat taataacgga ccggtcagat cgtttggatc
    15541 accaggccag ttttccactt ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt
    15601 aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg
    15661 gcctgaaccc aggataagca gtttactgtg tttggtcgtg cccatgagat ccccatagtt
    15721 gttggcagac aatgggcagg attgtaggga atttacagac gtaaaaaaag agtatgacga
    15781 ttttgttaac aatttgtgca atcggcagca tcgataagca ggtcaaattc tcccgtcatt
    15841 atcacctctg ctacttaaat ttcccgcttt ataagccgat taaatgatga ataaacgccc
    15901 ctgttaatga atatctggca tgttgtacta aaaatcgatg ttttgctttg acaatcccct
    15961 ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat tcttatatgc gcataaccat
    16021 gtatgtaaat accatgttta ccgtgctagt gaaatctacg tatggcgtgg acagacgcca
    16081 ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc accgtagacc caggcattgc
    16141 gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt agcgactctg aacagtgatg
    16201 tttcagggtc agacaggagt agggaaggaa tacagagaga caataataat ggtagatagc
    16261 aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta acattcttaa tgagttgcaa
    16321 aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg tgggactttc cccaacgccg
    16381 tgccttgagc gtgtgcgtcg gctggaaaga caagggttta ttcagggcta tacggcgctg
    16441 cttaaccccc attatctgga tgcatcactt ctggtattcg ttgagattac tctgaatcgt
    16501 ggcgcaccgg atgtgtttga acaattcaat accgctgtac aaaaacttga agaaattcag
    16561 gagtgtcatt tagtatctgg tgatttcgac tacctgttga aaacacgcgt gccggatatg
    16621 tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc tgcctggcgt caatgacaca
    16681 cggacatacg ttgttatgga agaagtcaag cagagtaatc gtctggttat taagacgcgc
    16741 taacacggaa caggtgcaaa atcggcgtat tttgattaca ctcctgttaa tccatacagc
    16801 aacagtactg gggtaacctg gtactgttgt ccgttttagc atcgggcagg aaaagcctgt
    16861 aacctggaga gcctttcttg agccaggaat acactgaaga caaagaagtc acattgacaa
    16921 agttaagtag cggccgccgc cttctggagg cattgctgat ccttattgtc ctgtttgccg
    16981 tctggttgat ggctgcctta ctaagcttta acccttcgga ccccagctgg tcgcaaacgg
    17041 cctggcatga acctatccat aatttaggtg ggatgcccgg tgcgtggttg gcagatacgc
    17101 tgttctttat ttttggcgtg atggcttaca ccattcccgt cattattgtc ggcggttgtt
    17161 ggtttgcctg gcgtcatcag tccagcgacg aatacattga ttattttgcc gtttcgctac
    17221 gcatcattgg cgttttggcg ctcatcctta cctcctgtgg tctggcggca atcaacgctg
    17281 acgatatctg gtattttgcc tccggtggcg tcattggcag cttactaagc actacgctac
    17341 aaccactgct acacagtagc gggggaacta ttgcgctgct ctgcgtttgg gcagcgggcc
    17401 tgacgttgtt caccggttgg tcatgggtga ccattgctga aaaactcggc ggctggattt
    17461 taaacattct caccttcgcc agtaatcgta cccgtcgcga tgatacctgg gtcgatgaag
    17521 atgagtatga agacgacgaa gagtatgaag atgaaaatca cggcaaacag catgaatcac
    17581 gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa acggttggcg gaaaaattca
    17641 ttaatccgat ggggcggcaa acagacgctg cgttgttctc cggtaagcgg atggatgatg
    17701 acgaagagat tacctacact gcacgcggtg tggctgccga cccggacgac gtcctatttt
    17761 cgggcaatcg tgcaacgcag ccagaatatg acgaatacga tccattatta aacagtgcgc
    17821 caattaccga acctgtcgct gtagcagctg ctgctaccac ggcgacacaa agctgggctg
    17881 cgccggttga acctgtgact cagacgccgc ctgttgcctc tgttgatgtt ccaccttcgc
    17941 aacctacagt agcctggcag cctgtaccgg gtccacaaac gggagagccg gttattgctc
    18001 ctgcaccgga aggttaccca cagcagtcac aatatgcgca gcctgcagtg caatataatg
    18061 agccgctgca acaaccagta cagccgcagc agccgtatta tgcacctgca gctgaacaac
    18121 ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc
    18181 aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagact gagcaaactt
    18241 atcagcagcc agccgctcag gagccgttgt accaacagcc gcaacccgtt gaacagcagc
    18301 ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg ccgctttact
    18361 attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg gcctggtatc
    18421 aaccgattcc agaaccggtt aaagaaccag aaccgatcaa atcttcgctg aaagcacctt
    18481 ctgttgcagc agtacctcca gtagaagccg ctgccgctgt ttctccgctg gcatctggcg
    18541 tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc
    18601 tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg gattggcccg cagttgccac
    18661 gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc gtcttacggt attaagctgc
    18721 cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca gcgcaatcag tatgattctg
    18781 gcgatcagta taacgatgat gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt
    18841 tcgcccagac acagcagcaa cgctatggcg aacagtatca acatgatgcg cccgtaaacg
    18901 cagaagatgc agatgctgcg gcagaggctg aactggctcg tcagtttgcg caaactcaac
    18961 aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg gatgattttg
    19021 aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgctg tttacgccaa
    19081 ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc acagcagcag tatcagcagc
    19141 cacaacagcc ggttgcgcca cagcagcagt atcagcagcc acaacagcag gttgcgccgc
    19201 agccacaata tcagcagccg caacaaccgg ttgcgccaca gcagcaatat cagcagccgc
    19261 aacaaccagt tgcgccgcag ccgcagtatc aacagccgca acaaccggtt gcaccgcagc
    19321 cgcagtatca gcagccacaa cagccagttg cgccacagcc gcaggatacc ctgcttcatc
    19381 cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa accgacgacg ccactgcctt
    19441 ctctggattt gctgacaccg ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg
    19501 aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt ccgtattaaa gccgatgtcg
    19561 ttaattactc tccggggcca gttatcactc gctttgaact taacctggct cctggcgtaa
    19621 aagcggcgcg catttctaac ttgtcacggg accttgcccg ttcactttcg acggtggcgg
    19681 tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg tctggagtta ccaaataaaa
    19741 aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc gataatccgt
    19801 cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt gccgatctgg
    19861 cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaaatct gtcggtgtga
    19921 acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt ttcatcatga
    19981 tcgacccgaa aatgctggag ctttcggttt atgaaggcat tccgcacctg ttaacggaag
    20041 tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac gagatggagc
    20101 gtcggtataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa
    20161 ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag ccgggtgaca
    20221 gtatggatgc ccagcatccg gtgctgaaaa aagaaccata cattgtcgtg ttggttgacg
    20281 aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata gctcgtctgg
    20341 cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aactcagcgt ccatcggttg
    20401 atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt accgtatcca
    20461 gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg ctgggtatgg
    20521 gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat ggtgcttttg
    20581 ttcgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt cgcccacagt
    20641 atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg tgcgggtggt ttcgatggcg
    20701 ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag
    20761 cgtcaatttc tggcgtacag cgtcagttcc gcattggtta taaccgtgca gcgcgtatta
    20821 tcgaacagat ggaagcgcag gggattgtca gcgaacaggg gcacaacggt aatcgtgaag
    20881 tgctggcccc accgccgttt gactaattaa tgcattgccg gataaggcgc ggtagcgtcg
    20941 catccggcac tctatcaact gaaaattcag tattttcttc tttcctcaag ctgattatta
    21001 gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg aggaataatg
    21061 atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg
    21121 gctgatgccg caagcgatct gaaaagccgc ctggataaag tcagcagctt ccacgccagc
    21181 ttcacacaaa aagtgactga cggtagcggc gcggcggtgc aggaaggtca gggcgatctg
    21241 tgggtgaaac gtccaaactt attcaactgg catatgacac aacctgatga aagcattctg
    21301 gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc tacggcaacc
    21361 tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa ccagtccagc
    21421 gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac gccgaaagcc
    21481 agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac aatccatcag
    21541 tttagcgcgg tggagcagga cgatcagcgc agcagttatc agctgaaatc ccagcaaaat
    21601 ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac ggtagatgat
    21661 caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg ataatacttt
    21721 tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg gtcagcaaca
    21781 tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatt tgcattctat
    21841 gatcctctgg gggccaccgg gtaccggcaa aacaaccctc gctgaagtga ttgcccgcta
    21901 tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggcgtgaaag agattcgcga
    21961 ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc cgcactattc tttttgttga
    22021 cgaagttcac cgtttcaaca aaagccagca ggatgcattt ctgccacata ttgaagacgg
    22081 caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta attcggcact
    22141 gctttcccgt gcccgtgttt atctgttgaa atccctgagt acagaggata ttgagcaagt
    22201 actaactcag gcgatggaag acaaaacccg tggctatggt ggtcaggata ttgttctgcc
    22261 agatgataca cgacgtgcca ttgcagaact ggtgaatggc gacgcgcgcc gggcgttaaa
    22321 tacgctggaa atgatggcgg atatggccga agtctatgat agcggtaagc gggtcctgaa
    22381 gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc cgctttgata acaaaggcga
    22441 tcgcttttac gatctaattt ccgcactgca taagtcggta cgtggtagcg cacccgatgc
    22501 ggcgctgtac tggtatgcgc gaattattac cgctggtggc gatccgttat atgtcgcgcg
    22561 tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc gatccacggg cgatgcaggt
    22621 ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg aacgcgccat
    22681 tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct acactgcgtt
    22741 taaagccgcg ctggccgatg ctcgcgaacg cccggattat gacgtgccgg ttcatttgcg
    22801 taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc gttatgctca
    22861 tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgccggaaa tagcacaaac
    22921 acgctattat ttcccgacaa acaggggcct tgaaggcaag attggtgaaa agctcgcctg
    22981 gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt atcgttgcgg
    23041 taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca tttaattcga
    23101 taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc
    23161 tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg ctcttgaaga
    23221 gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta actcccgatc
    23281 gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac gtctggaagt
    23341 gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt tacaggctga
    23401 aattcgcgat atcgcgctga ccatccctaa cctgcctgca gatgaagtgc cggtaggtaa
    23461 agacgaaaat gacaacgttg aagtcagccg ctggggtacc ccgcgtgagt ttgactttga
    23521 agttcgtgac cacgtgacgc tgggtgaaat gcactctggc ctcgactttg cagctgcagt
    23581 taagctgact ggttcccgct ttgtggtaat gaaagggcag attgctcgca tgcaccgcgc
    23641 actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg agaactatgt
    23701 tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga aatttgctgg
    23761 cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact atgcgctgat
    23821 cccaacggca gaagttccgc tgactaacct ggtgcgcggt gaaatcatcg atgaagatga
    23881 tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg gttcatatgg
    23941 tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa tggtgcagat
    24001 cgtgcgccca gaagactcaa tggcggcgct ggaagagatg actggtcatg cagaaaaagt
    24061 cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt tgcactggcg acatgggctt
    24121 tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca cctaccgtga
    24181 gatctcttcc tgctccaacg tttgggattt ccaggcacgt cgtatgcagg cacgttgccg
    24241 cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg gtctggctgt
    24301 tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc gtattgaagt
    24361 accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat acccaatttt
    24421 tctgaatcta aaaagcgcct ttagggcgct ttttttgtct ccctttgata ccgaacaata
    24481 attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24541 ttcaaattat tctatgtata caagcctata tagcgagctg ctatagaaat aattacacaa
    24601 tacggtttgt tactggaatc aatcgtgatc aagcttgagt gagccattat gaaaacgaaa
    24661 atccctgatg cggtattggc tgctgaggag agtcgccgtg gtttggtaaa aacgacagcg
    24721 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    24781 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    24841 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    24901 gtcgaaacga caataccggc gatgacaatt acgacggcct acactaggtt cgcgcctgcc
    24961 tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga ccgcctgaaa tatccgatga
    25021 aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg
    25081 acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag tctatctatc
    25141 tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccg ccgggaaata
    25201 ccctggtcgc gcgcctgatg aactgctgcg gcggttatct taaccattac ggtgactact
    25261 cttccgcgca aattgcggaa ggtttgaacc atacctacgg cggctgggca gatggtaaca
    25321 gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt tggtaataac ccaggcgaaa
    25381 cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcacgccag aaatctaatg
    25441 ctcgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt
    25501 ggatccctat tcgtccagga acagatgccg cactggttaa cggtctggcg tacgtcatga
    25561 tcactaaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc tatgacgaga
    25621 aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa agcttatatt ctgggtgaag
    25681 ggccagatgg cgtggctaaa acaccggaat gggcctcgca aatcaccggt gttccggcag
    25741 acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt atcagccagg
    25801 gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg
    25861 cgattctgac cggtaacgtt ggtattaatg gagggaacag cggcgcgcgt gaaggttcat
    25921 acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc agcatttcga
    25981 tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg cgtgatggtg
    26041 tgcgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat gccggtaact
    26101 gcctgattaa ccagcattct gaaatcaacc gtacccatga aatcctccag gatgataaga
    26161 agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa tatgctgaca
    26221 tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcactggat gcatcctgcg
    26281 ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga
    26341 ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa cagtttactg
    26401 aaggccgtac ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc
    26461 ctgaactgcc aacgtttgaa gagttccgca agcaggggat ctttaaaaag cgcgacccac
    26521 aagggcatca cgttgcttat aaagccttcc gtgaagatcc gcaggcaaac ccactgacta
    26581 cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc gctacctggg
    26641 aattgccaga aggcgatgtg atcgatccac tgccgatcta cacgccgggc tttgaaagtt
    26701 atcaggatcc gctgaacaaa cagtatccgc tgcagcttac aggtttccac tataaatctc
    26761 gcgttcactc aacttacggc aacgttgatg tgctgaaagc ggcttgccgt caggaaatgt
    26821 ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa cggcgataaa gtcaggatct
    26881 ttaacgatcg tggtgaggtt catattgagg cgaaagtgac gccacgaatg atgccgggcg
    26941 tggtcgcact gggtgaaggc gcctggtatg acccggatgc aaaacgtgtc gataagggcg
    27001 gttgtatcaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg aatccgtcac
    27061 atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga
    27121 ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac
    27181 tacaaagatt tgacgccaga agtcagtttc cgccgcattt atgaatatgc tggcggcgac
    27241 tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct gtcgatttcg
    27301 tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa
    27361 cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca ttggctgccg ttactgtcat
    27421 atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat gaccaaatgc
    27481 gatggttgtt atgaccgtgt tgcggagggt aaaaagccga tctgtgttga atcttgcccg
    27541 ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta aaaaacatgg cgatctggcg
    27601 gccgttgcgc cgttgctgcg agctcacttt accaaaccga atattgtgat caaacccaat
    27661 gccaatagcc gcccgaccgg ggataccact ggctatctgg caaacccgaa ggaggtgtga
    27721 gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg ggcaatgtgt
    27781 ggcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc gagcagaagc
    27841 ccagcagcgt gttatcgcct gcatgtttgg cttatgggta ctgatgggca ttggctttat
    27901 cgcctctatg ctccatcttg gttcaccgat gcgcgccttt aactcgctca accgggtagg
    27961 ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgctg taggcggcat
    28021 cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc tatggctggt
    28081 ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat
    28141 tgataccgtt ccgacctggt acagcatctg gacgccgatg ggctgggcga tgcgtctgct
    28201 gccagccatt tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac
    28261 agagctggca actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg
    28321 tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc
    28381 acagctaaaa ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct
    28441 ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc
    28501 cgtcgcaagc taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt
    28561 cgttaacgtg tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa
    28621 cgtcgccagt ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa
    28681 ccatgtcatc aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat
    28741 ttcattaaag gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc
    28801 tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt
    28861 tttacctgtc gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc
    28921 cgggcgagca atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc
    28981 gttaggaccg gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag
    29041 gtcacccagc gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga
    29101 aagtaaacca gcctggtgat caacaagcaa aacggcagca tcatttttat caagacgaac
    29161 atacggtttg gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag
    29221 acgcgatgca ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat
    29281 ttttcgactg aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga
    29341 ttaatcatct cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc
    29401 tgcgatatgc gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg
    29461 aaatttgaac ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt
    29521 tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc
    29581 tttggctcgc ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt
    29641 ttaccggcaa ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta
    29701 accgcagcta ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga
    29761 tgattggttt ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga
    29821 tttgggtggt tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt
    29881 tgcttgctgc gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca
    29941 gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag
    30001 ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga
    30061 cgtcaattac ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta
    30121 tctcaggaat cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag
    30181 gggtgagcaa tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg
    30241 gacaatggcc gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc
    30301 aggtctttgt cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag
    30361 cgttattcat cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcctgggctt
    30421 gtgagaaagt tgaacatcat caactggtgg cgatgaacca agccttactg ttgagctata
    30481 ccgtgggaag tctgcttggc ccgtcattta gcgctatgct aatgcagaat ttctccgata
    30541 atttattgtt tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgcttcgca
    30601 acgccggtca tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa
    30661 cttactgttt cacactctgc ttttttgttt cttctatctg acttgctttt ttccaaattt
    30721 tattcgttta aaaataaaat gtgcagcagg ttataatttt gcatttcgct atttccgcac
    30781 ttcttatttg ccgcgcataa tccctcgttt tacagatgcc cctttaattt tggcgaagga
    30841 tttgtctatg gctgggaatg ttcagga
//
LOCUS       DAJVQR010000041        30286 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVQR010000041 DAJVQR010000000
VERSION     DAJVQR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..805)
                     /gene="wcaD"
                     /locus_tag="OQM52_002002"
     CDS             complement(<1..805)
                     /gene="wcaD"
                     /locus_tag="OQM52_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1563865.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRL"
     gene            complement(780..1997)
                     /gene="wcaC"
                     /locus_tag="OQM52_002003"
     CDS             complement(780..1997)
                     /gene="wcaC"
                     /locus_tag="OQM52_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1563866.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1994..2482)
                     /gene="wcaB"
                     /locus_tag="OQM52_002004"
     CDS             complement(1994..2482)
                     /gene="wcaB"
                     /locus_tag="OQM52_002004"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1563867.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2485..3324)
                     /gene="wcaA"
                     /locus_tag="OQM52_002005"
                     /pseudo
     CDS             complement(2485..3324)
                     /gene="wcaA"
                     /locus_tag="OQM52_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3417..5579)
                     /gene="wzc"
                     /locus_tag="OQM52_002006"
     CDS             complement(3417..5579)
                     /gene="wzc"
                     /locus_tag="OQM52_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1563868.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5582..6025)
                     /gene="wzb"
                     /locus_tag="OQM52_002007"
     CDS             complement(5582..6025)
                     /gene="wzb"
                     /locus_tag="OQM52_002007"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1563869.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6031..7170)
                     /gene="wza"
                     /locus_tag="OQM52_002008"
     CDS             complement(6031..7170)
                     /gene="wza"
                     /locus_tag="OQM52_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1563870.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7479..7628
                     /locus_tag="OQM52_002009"
     CDS             7479..7628
                     /locus_tag="OQM52_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563871.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7829..9412
                     /locus_tag="OQM52_002010"
     CDS             7829..9412
                     /locus_tag="OQM52_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1563872.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9864..11717)
                     /gene="asmA"
                     /locus_tag="OQM52_002011"
     CDS             complement(9864..11717)
                     /gene="asmA"
                     /locus_tag="OQM52_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1563873.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11739..12320)
                     /gene="dcd"
                     /locus_tag="OQM52_002012"
     CDS             complement(11739..12320)
                     /gene="dcd"
                     /locus_tag="OQM52_002012"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1563874.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12412..13053)
                     /gene="udk"
                     /locus_tag="OQM52_002013"
     CDS             complement(12412..13053)
                     /gene="udk"
                     /locus_tag="OQM52_002013"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1563875.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13371..13613
                     /locus_tag="OQM52_002014"
                     /pseudo
     CDS             13371..13613
                     /locus_tag="OQM52_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13612..14553
                     /gene="mdtA"
                     /locus_tag="OQM52_002015"
                     /pseudo
     CDS             13612..14553
                     /gene="mdtA"
                     /locus_tag="OQM52_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14553..17675
                     /gene="mdtB"
                     /locus_tag="OQM52_002016"
                     /pseudo
     CDS             14553..17675
                     /gene="mdtB"
                     /locus_tag="OQM52_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17676..20741
                     /gene="mdtC"
                     /locus_tag="OQM52_002017"
     CDS             17676..20741
                     /gene="mdtC"
                     /locus_tag="OQM52_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1563876.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20742..22157
                     /locus_tag="OQM52_002018"
     CDS             20742..22157
                     /locus_tag="OQM52_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1563877.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22154..23557
                     /gene="baeS"
                     /locus_tag="OQM52_002019"
     CDS             22154..23557
                     /gene="baeS"
                     /locus_tag="OQM52_002019"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1563878.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23554..24276
                     /gene="baeR"
                     /locus_tag="OQM52_002020"
     CDS             23554..24276
                     /gene="baeR"
                     /locus_tag="OQM52_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1563879.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24467..24799
                     /locus_tag="OQM52_002021"
     CDS             24467..24799
                     /locus_tag="OQM52_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS1563880.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24946..26307
                     /gene="yegQ"
                     /locus_tag="OQM52_002022"
     CDS             24946..26307
                     /gene="yegQ"
                     /locus_tag="OQM52_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1563881.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26649..26966)
                     /locus_tag="OQM52_002023"
     CDS             complement(26649..26966)
                     /locus_tag="OQM52_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563882.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27372..28271
                     /gene="yegS"
                     /locus_tag="OQM52_002024"
     CDS             27372..28271
                     /gene="yegS"
                     /locus_tag="OQM52_002024"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS1563883.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28353..29126)
                     /locus_tag="OQM52_002025"
     CDS             complement(28353..29126)
                     /locus_tag="OQM52_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1563884.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29232..30272)
                     /gene="gatD"
                     /locus_tag="OQM52_002026"
     CDS             complement(29232..30272)
                     /gene="gatD"
                     /locus_tag="OQM52_002026"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1563885.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7007 a         8045 c         7827 g         7407 t
ORIGIN      
        1 ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat acagccagag
       61 aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc
      121 actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta
      181 tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt ttgatgctga
      241 gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc
      301 gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg
      361 aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg
      421 ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt
      481 gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga
      541 caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt
      601 taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc
      661 gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt
      721 cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc agcaggtagc
      781 tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg
      841 cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt
      901 ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt
      961 tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc
     1021 gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc
     1081 tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg
     1141 ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc
     1201 atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc
     1261 actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct
     1321 tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta
     1381 ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc
     1441 tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc
     1501 ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg
     1561 gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca
     1621 tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag
     1681 tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga
     1741 tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc
     1801 gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgacg
     1861 ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc
     1921 gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta
     1981 aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc accagcgcgt
     2041 tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg
     2101 tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac
     2161 atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat
     2221 cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc
     2281 ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat
     2341 acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg
     2401 aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt
     2461 tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca gacgtttgcc
     2521 gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat tgcggatctg
     2581 gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt tgtgcttgcg
     2641 gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca tttcgccgtg
     2701 attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt actccaccac
     2761 catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga acaaacactc
     2821 tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt tatagaacag
     2881 gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg aataaacttc
     2941 tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt gctgtttatg
     3001 ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga tcccggtgat
     3061 gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc cgctgttaat
     3121 gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact gttgcagttg
     3181 ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt agtcctggcg
     3241 caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg gcatatagat
     3301 tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg gtttgcttgc
     3361 cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag gcgtcgttat
     3421 ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc gctggcgcgg
     3481 cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc aaagcggctc
     3541 agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat taacgtggtt
     3601 ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg cgtatcaatc
     3661 aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg ttcgctcatt
     3721 aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa tttggcaata
     3781 gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc cgacagccca
     3841 ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc gcagtcgatc
     3901 aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca gacaaaggtt
     3961 ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc ctgcatcatc
     4021 gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc ggttggattc
     4081 cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt tttgacgcta
     4141 tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat tccgtgttct
     4201 tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag cacacccacg
     4261 atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt cttcggtttt
     4321 agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac ggtgctggct
     4381 tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg ctgaccagac
     4441 tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc cgttacgcga
     4501 ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag cgtgcggtac
     4561 gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt cagttcgttc
     4621 aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag cggcagatca
     4681 acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc gaggcggcta
     4741 cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc cgccgatttg
     4801 cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc gcggatctgt
     4861 tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc gttctccgtt
     4921 accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt ggtgacggta
     4981 aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc ttcttttttc
     5041 agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag ggtgtagttt
     5101 ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg gcggttaaag
     5161 gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc cgccccgaaa
     5221 atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac cgttttacca
     5281 agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg tttgttggct
     5341 aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat ttgtaccagt
     5401 gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca gagggcaaat
     5461 acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac caggcgacca
     5521 atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt ttctgtcatt
     5581 cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt tcaagtaatg
     5641 tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt tcacattcgt
     5701 tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc tcgcataagc
     5761 gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac aggcggcggc
     5821 tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg acgctgatag
     5881 cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc actttcagtt
     5941 ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata ttgccgacac
     6001 agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc tggctgtatc
     6061 cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac ggttccagcg
     6121 ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga attctgtcgc
     6181 catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa tcttgccgtt
     6241 acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt tggaggtggt
     6301 catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc taaagtccat
     6361 tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat catcattacg
     6421 cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt cgccgttttg
     6481 catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca ccacgttgcg
     6541 ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa tggtcagtgg
     6601 tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat aggccttttg
     6661 tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg tcgctaagcg
     6721 cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa ccttgccgat
     6781 atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc tggagctgcg
     6841 gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat taagaacgtc
     6901 ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca tattggggcg
     6961 cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg gataaacatt
     7021 caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt tgcccatcgt
     7081 cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg acaccaataa
     7141 tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc agagtaatta
     7201 tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt aattgaataa
     7261 aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat tgacagaata
     7321 attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt tagctaccaa
     7381 tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga tgagaaaggg
     7441 ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa tattggctcg
     7501 ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta acaattcggc
     7561 aattggctat ttttaagaat tatattaagt atcattcaat atggttttta ggagtttctt
     7621 taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat aatgttttat
     7681 tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa tatgagataa
     7741 atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg tgacatttgt
     7801 catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt ctatctgggc
     7861 cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc tggtctttat
     7921 tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg ttaccgggct
     7981 gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc tggtcaccct
     8041 gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact taatcatgct
     8101 gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac ggctggaagg
     8161 gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg tggtgacgca
     8221 aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg tcgggatggt
     8281 cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga tgttgatggc
     8341 cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc tctgcttaag
     8401 cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg tcattccgaa
     8461 aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca atcagttggc
     8521 tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc ggactaccga
     8581 agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg ccaaaaccgc
     8641 gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac ggcggatgat
     8701 tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt cgcgccacga
     8761 tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc tggagcgaaa
     8821 tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc tcggtgttgt
     8881 tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc tgcgggtgtt
     8941 gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc tggagcagtt
     9001 ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg tggaagggat
     9061 tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg aagtggaaga
     9121 gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg cgaatggtca
     9181 tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa aacgtgaata
     9241 tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac ctggcgaaga
     9301 agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc gcgtgcagaa
     9361 ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt gatggtatcg
     9421 gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg ttgtctgtgg
     9481 taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc gtttcctgta
     9541 ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat gttacctcgc
     9601 gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg cgatttaccg
     9661 ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt tcaccggctt
     9721 gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat cttacatatt
     9781 cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc
     9841 tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac atctttgcca
     9901 ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc ttcgtcctgt
     9961 agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg ccagttgcca
    10021 taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct ttccccgttc
    10081 cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc cagattcaac
    10141 atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt taacgtcacg
    10201 acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac gttatcgaag
    10261 ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat catctgctgg
    10321 aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc ttgccagttg
    10381 tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag tgacattttt
    10441 ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc aacgttctcc
    10501 agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt gcccggcagt
    10561 gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa acccgacttg
    10621 ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac gttactggcc
    10681 tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc atcaatacgc
    10741 gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc ggcaccgttt
    10801 tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg cgggaattgc
    10861 agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc gctcagcgta
    10921 ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga ctcctgccac
    10981 tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc accctgcaac
    11041 tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga agcatctacc
    11101 gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc ggagaactca
    11161 aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg gattgtcacc
    11221 tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag actggatata
    11281 tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc
    11341 ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac tgcccctttt
    11401 agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc cacgtccaga
    11461 cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag agacattcgc
    11521 ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc gagctgcaat
    11581 tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc attcggattc
    11641 accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat catcagcgtc
    11701 gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc gattcggctg
    11761 gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc
    11821 gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg acgcagcgcc
    11881 agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca gcccggatcg
    11941 atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga acgcccgtcc
    12001 agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc cagcgccagc
    12061 tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc gctcatcacg
    12121 cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa cgctgccgtg
    12181 tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc gccgttaata
    12241 cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca ggcttcaata
    12301 tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct taacgccaat
    12361 tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag cttattcaaa
    12421 gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc cgccgcgcgg
    12481 cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac
    12541 ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt taacgtcacg
    12601 cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg agaagttaag
    12661 ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga tgaccttctt
    12721 cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt aaaccggcag
    12781 gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc tgtgatccat
    12841 cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat ggctttgatc
    12901 tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt gctcacgtaa
    12961 ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc cagcgatacc
    13021 gataatgacg cactgatgag actgatcagt catatattta gcgacctgat taacctggat
    13081 gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca tccgtctgat
    13141 accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa ttcccggtgg
    13201 caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt gcgcaaatta
    13261 tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt ttgtactagc
    13321 ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta ataagcaaac
    13381 aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc agtttaggtt
    13441 tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc acccaactcg
    13501 ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat gccgggcgca
    13561 tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc attccaggaa
    13621 ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca gttcaaagtt
    13681 gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc taacgcccgc
    13741 cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg tcaggagctg
    13801 gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga tgaagcaagc
    13861 gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt cgatggtcgc
    13921 gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac caccggaatt
    13981 gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga aagcgatatc
    14041 gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc ctgggatcgc
    14101 accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca aatcgatgcc
    14161 acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc gctgtttccc
    14221 aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt agtgatccca
    14281 acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa tagcgaaaac
    14341 aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt ggtgatccgt
    14401 gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct gaccgaaggg
    14461 gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa agccaccagc
    14521 cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg agcagcacag
    14581 gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg atggtggcga
    14641 tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg ccggaagtgg
    14701 actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat gtcatgacct
    14761 ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg aaacagatgt
    14821 cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta acattaccgc
    14881 tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac ttgttgccga
    14941 gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg ccgatcatga
    15001 cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg gtggaaaccc
    15061 gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt tccggcggtc
    15121 agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc ggcctgacca
    15181 gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa ggtagcctcg
    15241 acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc gccgaagagt
    15301 atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc gatgtcgcaa
    15361 ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa gaacaggcca
    15421 ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc gacagcattc
    15481 ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg acagtgcttt
    15541 ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa ttgatgatgg
    15601 ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg gcgaccatca
    15661 ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg gtgtttctcg
    15721 atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga ttcgtggtcg
    15781 atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc gaaaaaccgt
    15841 tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg ctgaccttct
    15901 cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg cgactgttcc
    15961 gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg tcgctgaccc
    16021 tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa cagaacagct
    16081 tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt cgtggactgg
    16141 cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg ctgctgctta
    16201 gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac aacggcatta
    16261 ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc cagcgacaac
    16321 gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc tcatttgttg
    16381 gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac ctcaaaccgt
    16441 tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg gcggtagata
    16501 aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc gatactcagg
    16561 tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg ctcagtacct
    16621 gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat gtctccagcg
    16681 actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc gccagccgtc
    16741 tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt cagcgactga
    16801 tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac aataccgaaa
    16861 ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac ggtggcgtgg
    16921 tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc atcaaccatc
    16981 tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat tcgctgggcg
    17041 atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg gatatcacca
    17101 cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact gtctggctga
    17161 ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag agctttattc
    17221 acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg ctggcattaa
    17281 tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg ctgatcggta
    17341 ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag cgcgagcaag
    17401 gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt ccgatcctga
    17461 tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc ggggtcggcg
    17521 cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc agccaggtgc
    17581 tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca ttgtggacca
    17641 aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc ctcttcattt
    17701 accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc atactgggtt
    17761 tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt atggtcagcg
    17821 cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg ccgctggagc
    17881 gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg ctcggcagca
    17941 cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg cgtgatgtgc
    18001 aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc cgcccgacct
    18061 atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca tccgatactt
    18121 attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg atttcgcaaa
    18181 tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg cgcgtcgggc
    18241 tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc actgccatca
    18301 gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac cgctggcaga
    18361 tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt attcactaca
    18421 acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca gtgcaggatg
    18481 tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc cgcaaactgc
    18541 cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca gagttgcagg
    18601 agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc accattcgcg
    18661 cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg attctggtgg
    18721 tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg gtgccggttt
    18781 cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat aatctttcgc
    18841 tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg gtgctggaaa
    18901 acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg caaggtactc
    18961 gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg ttcctgccgc
    19021 tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg acgctttctg
    19081 tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg tgcggctgga
    19141 tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc cgcatgttgg
    19201 tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat acccgtctgg
    19261 tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa accttcttcc
    19321 cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt atttcgtttc
    19381 aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    19441 ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg tttatcaccc
    19501 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtga
    19561 agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat attcgcgttg
    19621 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    19681 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    19741 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca
    19801 tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac gccttcggtc
    19861 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    19921 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    19981 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg
    20041 tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg accggaaaat
    20101 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga
    20161 cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg aactcgcagg
    20221 tgatcctaat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    20281 gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt ggagcgctgt
    20341 tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat
    20401 taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac
    20461 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    20521 cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta ttgtcgggcg
    20581 gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt ctggtgatga
    20641 gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    20701 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat cttcccgaca
    20761 gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    20821 ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    20881 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    20941 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    21001 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    21061 tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg atgaaaatcg
    21121 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    21181 cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg cactggatct
    21241 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg ttaatgccga
    21301 actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg gcggttggca
    21361 tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga
    21421 ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    21481 ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    21541 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    21601 tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg
    21661 tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc tttggttatc
    21721 gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg tttatgacta
    21781 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca
    21841 actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    21901 cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    21961 ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc gacagcggca
    22021 ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg
    22081 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    22141 aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat
    22201 tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    22261 gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc tgttaagtga
    22321 tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca acaacgatcg
    22381 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    22441 gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa acaacaaagt
    22501 actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    22561 cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc gcaatactga
    22621 tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc tggcaacgtt
    22681 actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg taaaacgact
    22741 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    22801 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca cgctggagaa
    22861 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    22921 agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat tcacgccgga
    22981 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatcttca
    23041 tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat
    23101 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa
    23161 actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc gtttaatgca
    23221 gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg gcagcctgaa
    23281 aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    23341 tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg
    23401 caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    23461 ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca ttacagtaga
    23521 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    23581 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    23641 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actggcgtat
    23701 gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg caccgatggc
    23761 ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat ggtgacggca
    23821 aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga ctacatctgc
    23881 aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg ccgttgtaaa
    23941 ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga cgaaggtcgt
    24001 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgctt
    24061 aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt
    24121 tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa aaatctgcgc
    24181 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt
    24241 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta ctttgttagc
    24301 cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca gcaaactgac
    24361 attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca gtaaacagca
    24421 cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg ctggttggtt
    24481 tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg gcaatggtga
    24541 gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc
    24601 ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa gcaacggcaa
    24661 attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc agatgtacgc
    24721 caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca caagccagac
    24781 ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc ggcgctttac
    24841 cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc cctaaccgct
    24901 tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt taaaccggaa
    24961 ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta tggcgcagat
    25021 gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga attcaaccac
    25081 gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt ttatgtcgtg
    25141 gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct gaaaccggtg
    25201 gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat gctggtgcgt
    25261 gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt gaactgggcg
    25321 acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg cgagctgtcg
    25381 ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga gatcttcgtt
    25441 cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta tatcaacaag
    25501 cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa cgtccaggaa
    25561 gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc ggtgcaaaat
    25621 gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat cgaagaagcc
    25681 cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta catcatgaac
    25741 tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg cgtgcattcg
    25801 ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc acaggtttac
    25861 cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct gctggaaact
    25921 ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca tactcacgac
    25981 gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt tgttggtgag
    26041 tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa attctccgtt
    26101 ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct tgagcacatg
    26161 gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac tgtgtggctc
    26221 ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt ctccggggaa
    26281 accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg atggaaaatt
    26341 cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt cgctgaaaaa
    26401 cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg taatctccct
    26461 tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg ataaccgtct
    26521 tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac gtaacggtat
    26581 cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac gcttacaaaa
    26641 aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa tcctgcacct
    26701 tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa atgggctgca
    26761 tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga agcttcggct
    26821 tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc aagaccaaat
    26881 ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa aatactaatt
    26941 aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca acgacactct
    27001 ctagtgttta aatataataa tggcatgatt attataattg aataggatta taataaatgt
    27061 tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt cagggtaact
    27121 ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat attaggtata
    27181 tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc attgcaagct
    27241 cctggtggta accactgaat cctccatact tgaactgact ttttatcctc cgactttcat
    27301 cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca atcataaagg
    27361 cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt aaaagtactg
    27421 acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg acgatccatg
    27481 tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc cggaagttgg
    27541 gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt tctacggcgt
    27601 tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta ggaaccgcca
    27661 atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg aaactggcaa
    27721 ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc tgttttatta
    27781 atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa aaattaaaag
    27841 ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat actctgcaac
    27901 cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc ctggtcattg
    27961 gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac gcgttaatta
    28021 acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg gctctcgtat
    28081 caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg tcgtggtttg
    28141 atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg agtgggcaaa
    28201 acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtccat
    28261 tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg ccggatgcgg
    28321 tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc
    28381 ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat aatgtcaatt
    28441 ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga atcgagaacg
    28501 gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc ttcattaaag
    28561 gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta
    28621 atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt atggcggacc
    28681 gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa cgctgccggg
    28741 agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc gataagcatt
    28801 gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt gactgccgcc
    28861 tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa cccgacttcc
    28921 tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg aaagcgcgta
    28981 accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca
    29041 aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc
    29101 ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta
    29161 aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca gcatcacgct
    29221 ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg agcgatgtca
    29281 cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt
    29341 aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg
    29401 gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt
    29461 gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg ggcatgagga
    29521 ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc gaggataagc
    29581 tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg
    29641 ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga gctaatgtcg
    29701 atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag caggccaatg
    29761 gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa atgaaaagca
    29821 tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat atccgtgggt
    29881 agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc atcacgccgc
    29941 gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca ctctggacaa
    30001 gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa atcatcaaca
    30061 ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat tggataataa
    30121 tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt
    30181 tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc aacgcgcacg
    30241 ataccatcag tatcattcac tactgatttc ataaaaactc ctgatt
//
LOCUS       DAJVQR010000042        30035 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVQR010000042 DAJVQR010000000
VERSION     DAJVQR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            271..654
                     /gene="secE"
                     /locus_tag="OQM52_002027"
     CDS             271..654
                     /gene="secE"
                     /locus_tag="OQM52_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1563886.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            656..1201
                     /gene="nusG"
                     /locus_tag="OQM52_002028"
     CDS             656..1201
                     /gene="nusG"
                     /locus_tag="OQM52_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1563887.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1360..1788
                     /gene="rplK"
                     /locus_tag="OQM52_002029"
     CDS             1360..1788
                     /gene="rplK"
                     /locus_tag="OQM52_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1563888.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1792..2496
                     /gene="rplA"
                     /locus_tag="OQM52_002030"
     CDS             1792..2496
                     /gene="rplA"
                     /locus_tag="OQM52_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1563889.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2909..3406
                     /gene="rplJ"
                     /locus_tag="OQM52_002031"
     CDS             2909..3406
                     /gene="rplJ"
                     /locus_tag="OQM52_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1563890.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3473..3838
                     /gene="rplL"
                     /locus_tag="OQM52_002032"
     CDS             3473..3838
                     /gene="rplL"
                     /locus_tag="OQM52_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1563891.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4158..8186
                     /gene="rpoB"
                     /locus_tag="OQM52_002033"
     CDS             4158..8186
                     /gene="rpoB"
                     /locus_tag="OQM52_002033"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1563892.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8263..12486
                     /gene="rpoC"
                     /locus_tag="OQM52_002034"
     CDS             8263..12486
                     /gene="rpoC"
                     /locus_tag="OQM52_002034"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1563893.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12728..13861)
                     /gene="thiH"
                     /locus_tag="OQM52_002035"
     CDS             complement(12728..13861)
                     /gene="thiH"
                     /locus_tag="OQM52_002035"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1563894.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13858..14628)
                     /gene="thiG"
                     /locus_tag="OQM52_002036"
     CDS             complement(13858..14628)
                     /gene="thiG"
                     /locus_tag="OQM52_002036"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1563895.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14630..14830)
                     /gene="thiS"
                     /locus_tag="OQM52_002037"
     CDS             complement(14630..14830)
                     /gene="thiS"
                     /locus_tag="OQM52_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1563896.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14814..15569)
                     /gene="thiF"
                     /locus_tag="OQM52_002038"
     CDS             complement(14814..15569)
                     /gene="thiF"
                     /locus_tag="OQM52_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS1563897.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15562..16197)
                     /gene="thiE"
                     /locus_tag="OQM52_002039"
     CDS             complement(15562..16197)
                     /gene="thiE"
                     /locus_tag="OQM52_002039"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1563898.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16197..18092)
                     /gene="thiC"
                     /locus_tag="OQM52_002040"
     CDS             complement(16197..18092)
                     /gene="thiC"
                     /locus_tag="OQM52_002040"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1563899.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18173..18273)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18326..18802)
                     /gene="rsd"
                     /locus_tag="OQM52_002041"
     CDS             complement(18326..18802)
                     /gene="rsd"
                     /locus_tag="OQM52_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1563900.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18897..19670
                     /gene="nudC"
                     /locus_tag="OQM52_002042"
     CDS             18897..19670
                     /gene="nudC"
                     /locus_tag="OQM52_002042"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1563901.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19710..20774
                     /gene="hemE"
                     /locus_tag="OQM52_002043"
     CDS             19710..20774
                     /gene="hemE"
                     /locus_tag="OQM52_002043"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1563902.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20931..21602
                     /gene="nfi"
                     /locus_tag="OQM52_002044"
     CDS             20931..21602
                     /gene="nfi"
                     /locus_tag="OQM52_002044"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1563903.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21645..22235
                     /locus_tag="OQM52_002045"
     CDS             21645..22235
                     /locus_tag="OQM52_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1563904.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22422..22694
                     /gene="hupA"
                     /locus_tag="OQM52_002046"
     CDS             22422..22694
                     /gene="hupA"
                     /locus_tag="OQM52_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1563905.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22707..23402
                     /locus_tag="OQM52_002047"
     CDS             22707..23402
                     /locus_tag="OQM52_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1563906.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23404..23829)
                     /gene="zraP"
                     /locus_tag="OQM52_002048"
     CDS             complement(23404..23829)
                     /gene="zraP"
                     /locus_tag="OQM52_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1563907.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24067..25443
                     /gene="zraS"
                     /locus_tag="OQM52_002049"
     CDS             24067..25443
                     /gene="zraS"
                     /locus_tag="OQM52_002049"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1563908.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25440..26765
                     /gene="zraR"
                     /locus_tag="OQM52_002050"
     CDS             25440..26765
                     /gene="zraR"
                     /locus_tag="OQM52_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS1563909.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26762..28051)
                     /gene="purD"
                     /locus_tag="OQM52_002051"
     CDS             complement(26762..28051)
                     /gene="purD"
                     /locus_tag="OQM52_002051"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1563910.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28063..29652)
                     /gene="purH"
                     /locus_tag="OQM52_002052"
     CDS             complement(28063..29652)
                     /gene="purH"
                     /locus_tag="OQM52_002052"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1563911.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7030 a         7828 c         8265 g         6912 t
ORIGIN      
        1 gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata acatttgacg
       61 caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt accagaacct
      121 ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt tgagggcgta
      181 taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg gggtgaaaat
      241 gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc tcaaggaagc
      301 gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct ggcggcgatt
      361 gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc cgtagtaatt
      421 ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc taccattgct
      481 tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg ccaggaaaca
      541 ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat cctgtgggga
      601 ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt ctgagatgtc
      661 tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg aaggccgcgt
      721 agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt ttggtgaagt
      781 catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca aaagcgaacg
      841 taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga gctggcactt
      901 ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc gtcctgcgcc
      961 aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg gtgataagcc
     1021 gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg gtccgttcgc
     1081 tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga aagtgtctgt
     1141 ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg aaaaagccta
     1201 acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat tggaatacaa
     1261 tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata tcacggggag
     1321 cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa agtacaagcc
     1381 tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt aggtccggct
     1441 ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc aaaaactgat
     1501 tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga ccgttctttc
     1561 actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc aggtatcaag
     1621 tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc tcagctgcag
     1681 gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc gatgactcgc
     1741 tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga aatggctaaa
     1801 ctgaccaagc gcatgcgtgt tatccgtgag aaagttgatg caaccaaaca gtacgacatc
     1861 aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga aagcgtggac
     1921 gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg tggtgcaact
     1981 gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca aggtgcaaac
     2041 gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct ggctgaccag
     2101 atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc aatgcgcgtt
     2161 gttggccagc tgggccaggt tctgggtccg cgtggcctga tgccaaaccc gaaagtgggt
     2221 actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca ggttcgttac
     2281 cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt tgacgctgac
     2341 aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa accgactcag
     2401 gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc aggtgttgca
     2461 gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg cggtgatttt
     2521 gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga gatattcggg
     2581 ttgtggcaag gcggcaactg agtgagtcgc caagagcata gctaactatg tgactggtgc
     2641 gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa gatttgttcg
     2701 ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca agaaacttaa
     2761 tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg gcttgtttct
     2821 gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc cagagatttt
     2881 ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca aacaagcgat
     2941 tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg cggattcccg
     3001 tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag ctggcgtata
     3061 catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc cgttcgagtg
     3121 cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac acccgggcgc
     3181 tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg tcaaagccgc
     3241 tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa ctctgccgac
     3301 ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg ctggcaaact
     3361 ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc agttatcttt
     3421 ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt aaatgtctat
     3481 cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg ttgtagaact
     3541 gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag ctgtagctgc
     3601 tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga aagctgctgg
     3661 cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg gtctgaaaga
     3721 agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga gcaaagacga
     3781 cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag ttaaataagc
     3841 caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt ttagtcacca
     3901 gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact gctgtgcctt
     3961 tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt tctgtgtaaa
     4021 tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt tccattttcc
     4081 ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt cgacttgtca
     4141 gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt cgtaaggatt
     4201 ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag cttgactcgt
     4261 ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct gctttccgtt
     4321 ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc agctaccgcc
     4381 ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc tattccgcac
     4441 cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa ggcaccgtaa
     4501 aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca gacaacggta
     4561 cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt agtccgggcg
     4621 tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg tataacgcgc
     4681 gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag gacaacctgt
     4741 tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc gccctgaact
     4801 acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa atccgtgata
     4861 acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca tcttttgaca
     4921 tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg cgccacattc
     4981 gccagctgga aaaagacgac gtcaaactga ttgaagtccc ggttgagtac atcgcaggta
     5041 aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc gcagcgaaca
     5101 tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag cgtatcgaaa
     5161 cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta cgtgtcgacc
     5221 caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc cctggcgagc
     5281 cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc gaagaccgtt
     5341 atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc gaagaaatcg
     5401 aaggttccgg tattctgagc aaagacgaca tcattgatgt tatgaaaaag ctcatcgata
     5461 tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt cgtatccgtt
     5521 ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta gagcgtgcgg
     5581 tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat atgatcaacg
     5641 ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg tctcagttta
     5701 tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc gcactcggcc
     5761 caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac ccgactcact
     5821 acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg atcaactctc
     5881 tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat cgtaaagtga
     5941 ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa ggcaactacg
     6001 ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa gacctggtaa
     6061 cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac tacatggacg
     6121 tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg gaacacgatg
     6181 acgccaaccg tgcattgatg ggtgcgaata tgcaacgtca ggccgttccg actctgcgcg
     6241 ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac tccggtgtaa
     6301 ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt atcgttatca
     6361 aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac aacctgacca
     6421 aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg tctctgggtg
     6481 aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc ggtgaactgg
     6541 cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac ttcgaagact
     6601 ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc cacattcagg
     6661 aactggcgtg tgtgtcccgt gacaccaagc tgggtccgga agagatcacc gctgacatcc
     6721 cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt tacattggtg
     6781 cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt gaaactcagc
     6841 tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct gacgttaaag
     6901 actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt caggtcttta
     6961 ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg cagctcaaac
     7021 aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg ttcagccgta
     7081 tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa ctgccgcgcg
     7141 atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg gaacagctgg
     7201 ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg aaacgccgca
     7261 aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag gtatatctgg
     7321 cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt aacaagggtg
     7381 taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt acgccggtag
     7441 acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag atcctcgaaa
     7501 cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg ctgaaacagc
     7561 agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg ggcgctgacg
     7621 ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt ctggctgaaa
     7681 acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa gaagcagaaa
     7741 ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc ctgtacgatg
     7801 gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac atgctgaaac
     7861 tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac agcctggtta
     7921 ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg gagatggaag
     7981 tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc actgttaagt
     8041 ctgatgacgt gaacggtcgt accaagatgt ataaaaacat tgtggacggc aaccatcaga
     8101 tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt tcgctgggta
     8161 tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct gtcggggtaa
     8221 aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga tttattaaag
     8281 tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc tctggcttcg
     8341 ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac catcaactac
     8401 cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg gccggtaaaa
     8461 gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt catctgtgag
     8521 aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg ccacatcgaa
     8581 ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg tatcggtctg
     8641 ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc ctatgtggtt
     8701 atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga gcagtatctg
     8761 gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga agcaatccag
     8821 gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga agagctgaac
     8881 gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact gctggaagcg
     8941 ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc ggtactgccg
     9001 ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc tgacctgaac
     9061 gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct ggatctggct
     9121 gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt agacgccctg
     9181 ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc tctgaaatct
     9241 ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct cggtaagcgt
     9301 gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct gcatcagtgc
     9361 ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg caagctggaa
     9421 ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg cgaagaagct
     9481 gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct gaaccgtgca
     9541 ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga aggtaaagct
     9601 atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg tgaccagatg
     9661 gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct gatgatgtct
     9721 accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc tcaggacgtt
     9781 gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga aggcatggtg
     9841 ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc tctgcatgcg
     9901 cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt agtagcgaaa
     9961 accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt accgaaaggt
    10021 ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa aatgctgaac
    10081 acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca gatcatgtac
    10141 accggcttcg cctatgcagc gcgttctggt gcatctgttg gcatcgatga catggtcatc
    10201 ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga aattcaggag
    10261 cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat cgatatctgg
    10321 gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac tgaaaccgtt
    10381 attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta catgatggcc
    10441 gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat gcgtggtctg
    10501 atggcaaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt ccgtgaaggt
    10561 ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct ggcggatacc
    10621 gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt ggcgcaggac
    10681 ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac tccggttatc
    10741 gaggggggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt aactgctgaa
    10801 gacgttctga agccgggtac tgctgatatt ctcgttccgc gcaacacgct gctgcacgaa
    10861 cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg ttctgttgta
    10921 tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct ggcgcgtggc
    10981 cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat cggtgaaccg
    11041 ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg tgcggctgct
    11101 gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt gaagtcggtt
    11161 gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa actgatcgac
    11221 gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact ggcgaaaggc
    11281 gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca caccatgccg
    11341 gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg ccagaccatt
    11401 actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga ttccgcagaa
    11461 cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc tcagggtaac
    11521 gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg taaagcgatt
    11581 gttcagctgg aagatggcgt acagatcagc tctggtgaca ccttggcgcg tattccgcag
    11641 gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga cctgttcgaa
    11701 gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt ttccttcggt
    11761 aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag cgatccgtac
    11821 gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg tgtagaacgt
    11881 ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct gcgtggtgtt
    11941 catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct gcagggcgta
    12001 aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa agctaccatc
    12061 gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc tcgcgtcaag
    12121 atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc ccgcgatctg
    12181 ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc atcgttccag
    12241 gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga actgcgcggc
    12301 ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta cgcgtaccac
    12361 caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca ggtgactgca
    12421 gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg ttctgataac
    12481 gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatgggtg
    12541 ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga aacgaggaag
    12601 aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa acacaacgct
    12661 ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta cgttcttaca
    12721 ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct ttccataccg
    12781 gctgcaaacc ctgagcggtt aacacggcag caaccgcttc cggtctgcga tcgtcgtgcg
    12841 gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc gttttcgaga
    12901 aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac ggtgattccc
    12961 gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag atggtttgca
    13021 ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac gggcgcaggc
    13081 gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat agcaaatgtt
    13141 cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc gcgccgaggc
    13201 ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc cagaagaagt
    13261 cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat gtctcctgat
    13321 aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc tccgccagcg
    13381 gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga tgacgacgaa
    13441 agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga tgttcaaagc
    13501 ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc gttttgcgct
    13561 tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa agattggaaa
    13621 gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc agacgctgcg
    13681 cctgttgggc cagttgttcc agatagccac tggcggcagg cgataacagc gccatcatgt
    13741 cgtcgcgggt gagttgcgag gcatttagcg cccgctctac gtcagcagcc gttttgccgt
    13801 tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgattgctg aaggttttca
    13861 tgccgatgcc tccagaaatc cggtcagcgg gctggtggca tgagcaaaat aactgcggct
    13921 gcccggtccg gactgacgtg ccagcaggcc tacttctacc gccagacgaa atgccttcgc
    13981 catgttgacg ggatcgtccg cgacggcaat cgccgtattc actaacaccg cgtcggcccc
    14041 catttccagc gcctgcgtgg catggctggg aacgccgatg ccagcatcga caaccaccgg
    14101 aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc cctgattcga
    14161 gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca gacgtttaca
    14221 caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta ccagcgtttc
    14281 ggcggctttc agggtttcga tgggatcggg caacagccag cgggcgtcag ggtgaatctc
    14341 caattttagc cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga aaatggcttc
    14401 ttccgctgtt ttcgccccgg aagtattcgg cagcagggcc acacccgccg cgataagcgg
    14461 ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca gtgtcaccag
    14521 ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga atttccctgt
    14581 gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct caaccccctg
    14641 caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc gtccactgct
    14701 cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt tggtcgagtt
    14761 gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga tcgttaaaca
    14821 ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa cgccaggctg
    14881 cgccactggc tcgacttacc gtcgaacagt cggagttctc ccgcaggcgt ctctataccg
    14941 cttaataact taatggcttc cagtgcctgc aaagtgccca tcaccccgac caccgggcca
    15001 accacgcccg ccgtgcggca gttgcgttct ggctgctggt tatctggcca caggcagcgg
    15061 taacacccct gctcccaggg cggtgtcagt accatcaact gaccgccaaa tccgaccgcg
    15121 ctggcggtga taagcggcgt gttgagtgcc acgcaggcgg cattaatctc ctggcgagtc
    15181 gccatattgt cggtacagtc gagcaccaca tcggcttgtg caaccgcatc tttcaacgcc
    15241 tcacccgtta accgttgttg taatgctgtc agttgaatgt cgggattcaa ctgtgtcagt
    15301 cgctgttggc tgacctgcga tttcgggctg ttgatatctt cagtggtaaa gaggatttgt
    15361 cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt cccgacgcca
    15421 gcccctgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat aatcagcacc
    15481 tggctgtcga gcagtttttg ctgcccgtca agagcgatat cgtcgagcag aatttggcgg
    15541 ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca gcaactgtgc
    15601 cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga tactgccgac
    15661 acccgttgct atcaccgcag gcgcgcgtgc cagactgata ccgccaatcg ccacggtggg
    15721 ataatccgcc aggcgctcaa catgccgtgc caactgttcc agcccctgcg gtgcagaagg
    15781 catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg ggcgtgctgc
    15841 cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca ggcctgccgc
    15901 gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga cgccatacgc
    15961 ctgatgcttg atcgcgagcc gccagtaatc gttgataaac aatcgcgcgt tatagcggcg
    16021 gcccagcgca attgccgcca cgacatcggc ttccacctct tcatcgcgcc gatctttgat
    16081 gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact gtacgctgtc
    16141 caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct gatacatcac
    16201 gcttcctcct tatgcaggta gatttctccg cctctggcac ggaagttctc cgacatatcc
    16261 gccattccca cttcaatggt ttgcgcggcg gcgtaatcgc gtacttcctg gctgattttc
    16321 atcgagcaga attttggccc acacatggag caaaaatggg cgactttgcc ggactcttgg
    16381 ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag attaaactgg
    16441 tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat ttgcgcgccc
    16501 ggatgccctt ttgccaggtc agcagcgtgg gcggcaatct tataagtgat aagcccctgc
    16561 ttaacatctt ctttattggg cagacccaga tgctcttttg gcgttacgta acagagcatc
    16621 gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg gtcatagccc
    16681 ggtgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg gcagtgctct
    16741 aactcctcgg tcatattgcg gcggatcatc tgcatcggca catggcctgg gccttcaatc
    16801 atcacctgta cgtcatattc ccaggcgatt ttggtcagtt cgcccagcgt atgcaattcg
    16861 gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc gtcgcccagc
    16921 gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg ttgatagagg
    16981 aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc gcgagagacg
    17041 ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc cgcatggata
    17101 gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa cgcttcccag
    17161 gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg cactgtaccg
    17221 atcggcaccg gactgttacg caaaatccac tcgcgggttt cgtgaatata gcgaccggtg
    17281 gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt ttccacttct
    17341 tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt taccaggaag
    17401 ttacggccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg gataatcgca
    17461 cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg cagatgtgcg
    17521 ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg ctcgcggccc
    17581 atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg gcgggcgtag
    17641 tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa gccgctaaaa
    17701 cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga actgcgcacg
    17761 gtaagttctt cggtatcggc gcgcgcatcg atccacggct ggcgtagttt tgccagccct
    17821 tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc gtagaccgga
    17881 atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag cgtcgggcta
    17941 agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat ataaatgcgt
    18001 tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc ccgggcgcgt
    18061 tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtt aaggacgtgg
    18121 cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg atagcagatt
    18181 aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc gaataaacgg
    18241 tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg cgttgaatgt
    18301 aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg gcgtcaagca
    18361 ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg ccgatgtcag
    18421 ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc aggctggaat
    18481 cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc ttcgcggcgc
    18541 gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata ctgaaatgtc
    18601 cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt tcgtttagcc
    18661 tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc acgagcagat
    18721 gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg cgctccgtca
    18781 ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa actgctcagt
    18841 gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca agagctatgg
    18901 atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa caaaaattat
    18961 ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc
    19021 gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttaatacaa cagcagcggc
    19081 gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg tttcaactgg
    19141 ccggacgtgg cgtgcaactg gcggagtttt accgatcgca taaatactgt ggttactgcg
    19201 ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgtagccat tgccgtgagc
    19261 gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat gattcgctcc
    19321 tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc ggattcgtcg
    19381 aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag agcggaatta
    19441 aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag tctttaatga
    19501 ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa gaattgctcg
    19561 aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc accgtagcgc
    19621 gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga tgatacactg
    19681 accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat cgttatctgc
    19741 gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc caggcgggtc
    19801 gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg tcgctgtgca
    19861 aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac ccgctggatg
    19921 cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta gggctctatt
    19981 ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc gacgtcgata
    20041 aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca
    20101 ttcgtcacga actgaaaggc gaagtgccgc tgattggttt ttccggcagc ccgtggacgc
    20161 tggcaaccta catggtggaa ggcggcagca gcaaagcctt caccgtgatc aaaaaaatga
    20221 tgtatgccga tccgcaggcg ctgcacgctc tgctcgataa actggcgaaa agcgtcactt
    20281 tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc gacacctggg
    20341 gcggcgtgct taccgggcgc gattatcaac agttctcgct ctattacatg cataaaattg
    20401 ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg tttaccaaag
    20461 gcggcggaca gtggctggaa gccatggctg aaaccggttg cgatgcgctg ggcctcgact
    20521 ggacaacgga catcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg ttgcagggta
    20581 atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa gtagcgacta
    20641 tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac ggcattcatc
    20701 aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga ctgtctgaac
    20761 agtatcaccg ctaagagcct gatccatcag gctattttat ttgccatttt gaggccgggc
    20821 agtgctcaaa atcctcacgt actacgtgta cgctccggtt tttccgcgct gtccggcctc
    20881 aaactgcctt caacaattac gcctgttgaa ccaggctcta aggagcgatt atggatctcg
    20941 cgtcattacg cgctcaacaa atcgaactgg cttcttctgt gatctgcgag gatcgactcg
    21001 ataaagatcc accggatctg atcgccggag ccgatgtcgg gtttgagcag ggcggagaag
    21061 tgacgcgagc ggcgatggtg ctgctgaaat atccctcgct tgagctggtg gagtataaag
    21121 ttgcccgcat cgccaccacc atgccttaca ttccaggttt tctttccttc cgcgaatatc
    21181 ctgcgctgct ggcagcgtgg gagatgctgt cgcaaaagcc ggatttagtg tttgtcgatg
    21241 gtcatgggat ctcgcatcct cgccgtcttg gcgtcgccag ccattttggc ttattggtgg
    21301 atgtgccgac cattggcgtg gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca
    21361 gcaaaccggg cgcgctggcc ccactgatgg ataaaggcga gcagctggcc tgggtctggc
    21421 gcagcaaagc gcgctgtaac ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca
    21481 gcgcgctggc gtgggtacaa cgctgcatga aaggctatcg tctgccggag ccaacgcgct
    21541 gggcggacgc ggtggcctcg gaacgtccgg cgttcgtgcg ctatacagca aatcagccct
    21601 aattcaggta aactgcggat aatttccgta tttgagaact caacatgtta caaaacccaa
    21661 ttcatctgcg tctggagcgc ctggaaagct ggcagcatgt cactttcatg gcttgcttat
    21721 gcgaacgcat gtaccccaat tacgccatgt tctgccagca aaccggtttt ggtgatgggc
    21781 aaatttaccg tcgtattctc gatctcatct gggaaacgct gaccgttaaa gacgcaaaag
    21841 taaatttcga cagccaactg gagaaatttg aagaagcgat tccttcagcc gacgatttcg
    21901 atctgtacgg cgtttatccg gcaatcgatg cctgcgtggc gttaagtgaa ctggtccatt
    21961 cgcgtttgag tggtgaaacg ctcgaacacg cggtggaagt gagtaagacc tccatcacga
    22021 ccgttgcgat gctggaaatg actcaggctg gtcgcgaaat gggcgatgaa gagcttaaag
    22081 aaaacccagc tgtagagcaa gaatgggaca ttcagtggga aatattccga cttttagctg
    22141 agtgcgaaga acgcgatatc gagctgataa aaggccttag ggcagacctg cgtgagtcgg
    22201 gtgagagcaa tattggtata atttttcagc aataagacca gaaaacgtga tttaacgcct
    22261 gatttgtcgt acctggagtc ttccctttcg ccccccgtct ggtctacatt tggggggcga
    22321 aaaaaagtgg ctatcggtgc gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac
    22381 tcgatgctta gcaagcgata aacacattgt aaggataact tatgaacaag actcaactga
    22441 ttgatgtaat tgcagagaaa gcagaactgt ccaaaaccca ggctaaagct gctctggagt
    22501 ccactctggc tgcaattact gagtctctga aagaaggcga tgctgtacaa ctggttggtt
    22561 tcggtacctt caaagtgaac catcgcgctg agcgtactgg ccgcaacccg cagaccggta
    22621 aagaaatcaa aattgccgca gctaacgtac cggcatttgt ttctggcaag gcactgaaag
    22681 acgcagttaa gtaagattgc gtggcagtga acagttttaa cgaaggggtg gtttcacccc
    22741 ttttgtcttt ctggcgtcga tcattgatgc tggcaggtgc gctgtttctc actgcctgta
    22801 gccacaactc ttcacttcct ccctttaccg ccagtggatt tgctgaagac cagggcgcgg
    22861 tacgcatctg gcgaaaagac agcggcgata atgtgcatct gcttgccgtg tttagcccgt
    22921 ggcgcagtgg cgataccacg acgcgagagt atcgctggca gggcgataac ctcacgctca
    22981 tcaatatcaa tgtttacagc aaaccgccgg tgaacattcg cgcgcgtttt gacgatcgcg
    23041 gcgatctgag ctttatgcaa cgtgaatccg atggggaaaa gcagcagctt tctaacgacc
    23101 aaatcgattt ataccgttat cgtgctgatc agatccgcca gattagcgat gccttacgtc
    23161 aggggagagt cgtgctgcgc caggggcgct ggcatgcgat ggaacagacc gtgaccacct
    23221 gcgaagggca aaccattaaa cctgatttag attcgcaggc gatagcgcat atcgagcgcc
    23281 gccagagccg ctcttctgtt gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc
    23341 aattactgtt agtggcaaac tctgatttct gtcgctggca acccaacgag aaaacgttct
    23401 gatttaccag tggcccatac ccatatgacc gccaccaccg cagccgccgt agcccatgcc
    23461 cattccggct ccgcgcggaa tacccgcttc agccatcgcg atatctcgtt tcacccgtaa
    23521 ctcatctaac gactgacgca aattctccat ctctttggcg accgcgttaa ttttgctgct
    23581 atccggtggg ttcgcggcta acagggcatt gtattcataa cgcttcgtca ccagttgctg
    23641 ttgcagtgcg ctgctttgag cgtaaaagtc attatggatt ttctgccacg ctgtctgttg
    23701 ttcgctggtc aaaggcgcgg cattttgctg ccacataccg tgtccgccgt gagcaaatgc
    23761 agatgtcgat cccatcgcca ttgctgaaag cgccatcatt accagggcaa ttttcgtgtt
    23821 ccgtttcatg gttaatcctc cagtggttgt cttacttcgg gtattgcatc ttccgtgcca
    23881 acgatgaaac gctgatatga cgggtaatct ggcatgataa acgagtaaaa atgactcgcc
    23941 tgctgcgggt agcgagtcat ttttactcat tgaaacttaa gcctttgtgt tacagcgcag
    24001 ggtaagcgct gataaaagat ggcatgattt ctgctgtcag aaagggatga gcaggcaaag
    24061 aagaagatgc gttttatgca acgttctaaa gactccttag ctaaatggtt aagcgcgatc
    24121 ctccccgtgg tcattgttgg gctggtaggg ttgtttgcgg tgacggtgat tcgcgattat
    24181 gggcgcgaga ctgccgccgc cagacaaacg ctgctggaaa aaggcagtgt acttattcgc
    24241 gctcttgaat ccggctcgcg cgtcggcatg gggatgcgca tgcatcatgc gcagcagcag
    24301 gccttactgg aagaaatggc cgggcagcct ggagtacgtt ggtttgcggt cacggatgaa
    24361 caaggaacaa tcgtgatgca tagcaactcc ggcatggtgg gaaaacagct ttattcccct
    24421 caggaaatgc agcagttaca tccgggagat gaagaagcgt ggcggcggat cgatagcgca
    24481 gacggcgagc ctgttctgga aatttatcgc cagtttcaac cgatgtttgc tgctggaatg
    24541 taccggatgc gccatatgca gcagtatgcc gcgacaccac aagcaatttt catcgctttc
    24601 gacgccagta atattgtgag tgccgaagat cgtgagcaga gaaacaccct gattattctc
    24661 ttcgccctgg cgacggtctt gctggcaagc gtattgtcat tcttctggta tcgccgctat
    24721 ctgcgctcgc gccagcttct acaagatgaa atgaagcgca aagagaagct ggtggcgctg
    24781 gggcatcttg cggcaggcgt tgcccacgaa atccgtaacc cactttcctc gattaaagga
    24841 ctggcgaaat actttgccga gcgcgcgcct gcagggggag aagcgcatca actggcgcag
    24901 gtgatggcga aagaggccga ccgtttaaac cgcgtggtaa gcgagttgct ggaactggtt
    24961 aagccaacgc atctggcttt gcaggcggtg gatctcaaca cgctgattaa ccactcatta
    25021 cagctggtaa gccaggatgc aaacagccgg gagatccagt tacgctttac cgccaacgac
    25081 acattaccgg aaattcaggc cgatccggac aggctgactc aggtcctgtt gaatctctat
    25141 ctcaatgcta ttcaggcgat tggtcagcat ggcgtgatta gcgtgacggc cagcgaaagc
    25201 ggcgcgggcg tgaaaatcag cgttaccgac agcggtaagg gaattgcggc agatcagctt
    25261 gaagccatct tcactccgta cttcaccacc aaagccgaag gcaccggatt ggggctggcg
    25321 gtcgtgcata atattgttga acaacacggt ggtacaattc aggtcgcaag ccaggaggga
    25381 aaaggctcaa cgttcaccct ctggcttccg gtcaatatta cgcgtaagga cccacaagga
    25441 tgacgcacga taatatcgat attctggtgg tggatgatga cattagccac tgcactattt
    25501 tgcaggcttt actgcgcggc tggggctata acgtcgcgct ggcgaacagc gggcgacagg
    25561 cgctggagca ggtgcgggaa caggtttttg atcttgtgct ttgcgatgtg cgaatggcgg
    25621 agatggacgg catcgccacg ctgaaagaga tcaaagcgtt aaacccggca attccggtgc
    25681 tgattatgac tgcgtactcc agcgtcgaga cggcggtaga ggcactgaaa actggggcgc
    25741 aggattatct catcaagccg ctggatttcg ataacctaca ggcgacgctg gaaaaggcgc
    25801 tcgcgcatac gcacagtatt gatgctgaaa cacctgcggt gactgccagc cagttcggta
    25861 tggtcggtaa aagcccggcg atgcaacacc tgctcagtga aatcgccctc gtcgcgccat
    25921 cggaagccac ggtgctgatc cacggcgatt ccggcaccgg taaagagctg gtcgccaggg
    25981 cgattcacgc cagtagcgca cgtagcgaaa aaccgctggt aacgctcaac tgtgcggcac
    26041 tcaacgaatc cttgctggaa tctgaattgt tcggtcacga aaaaggggcg tttactggag
    26101 ccgataaacg ccgggaaggg cgctttgttg aggcggacgg cggcacgctg tttctcgatg
    26161 aaattggcga tatctcgccg atgatgcagg tgcgtctgct gcgtgcgatt caggagcgcg
    26221 aagttcagcg tgtcggtagc aaccagacta tctcggttga tgtccggctg attgcggcga
    26281 cccatcgcga tcttgccgca gaggtgaatg ccgggcgttt tcgccaggat ctctactatc
    26341 gcctgaatgt ggtggcgatt gaagtaccat cgctgcggca acggcgggaa gatatccctc
    26401 tgctggctgg ccattttctt cagcgctttg ccgagcgtaa tcgcaaggcg gtaaaaggct
    26461 ttacgccaca ggcgatggat ttgctgatcc actacgactg gccgggaaat attcgtgaac
    26521 tggaaaacgc ggtggaacgg gcggtggtgc tgctgaccgg ggaatatatt tccgaacgcg
    26581 agctgccgct ggcgattgcc agtacgccga tcccgctggg gcaaagtcag gatattcagc
    26641 cgctggtgga agtggaaaaa gaggtgattc tggcggcgct ggagaaaacg ggcggcaaca
    26701 aaaccgaagc cgcccgtcag ttagggatca cgcgcaaaac gctattggca aaactgtcgc
    26761 gttagttctg ctcgcgttcg atggcgcgcc aaccgatatc tttccggcag aagcaatcat
    26821 cccagtggat atcggtcatt aaggcgtagg cgcgtttctg cgcttctgcc acggtatgac
    26881 ccagcgcggt gacgcacagt acgcgcccgc cgctggttac cacctgctcg tcatccgcca
    26941 gttttgtgcc cgcgtggaac actttgccgt cagccacttc ttccagcggc aggccgtgga
    27001 tcacatcgcc ggtgcggtaa tcgcccggat atccacccgc agccatcacc acgctgagag
    27061 aagcacgttc atcccactct gacgttttct cgtccagttt gccttcacag gccgccaggc
    27121 agagctcaac cagatcggac ttcatgcgca gcataatcgg ctgggtttcc ggatcgccaa
    27181 agcggcagtt aaattcaata accttcggat tgccctgttt gtcgatcatc aggcccgcat
    27241 agagaaaacc ggtgtaggtg ttgccttccg ccgccatgcc tttcacggtt ggccagatga
    27301 tacgttccat ggtgcgctga tgaacttcat cggttactac cggcgcgggg gagtaagcgc
    27361 ccatcccgcc ggtgtttggt ccgttatctt tatcgcctac gcgtttgtga tcctggctgg
    27421 tagccatcgg cagcacatgc tcgccgtcca ccatcacgat aaagctcgct tcttcgccat
    27481 cgaggaactc ttcgataacg atgcgatgac ccgcgtcgcc aaaagcgttg cccgccagca
    27541 tatcgtgaac agccgcttcc gcttcttcca gcgtcatcgc cacgataacg cctttcccgg
    27601 cagccagacc gtccgcttta atgacgattg gcgcgccttt ctcacgcaga tacaccagcg
    27661 caggttctac ctcggtgaag ttctggtatt ccgccgtagg gatcttatgg cgggccagga
    27721 aatctttggt aaacgctttt gagccttcca gttgggccgc acctgcggtt gggccgaaga
    27781 ttttcagccc ggcggcgcgg aaggtatcga ccacgccttt caccagcggc gcttccgggc
    27841 cgacgatggt cagatcaatc ttttcgtttt gtgcgaaatc caacagcgcc gggatatcgg
    27901 tcacgccgat agcaacgttt tgcagcgcgg gttccagcgc agtgcctgca ttacccggag
    27961 cagcaaaaac agtctcaacc agcggcgact gggccgcttt ccaggccagc gcgtgctcgc
    28021 gcccgccgtt accaatcact aatactttca tctattgctc cattaatggc ggaagtggcg
    28081 catgtcggtg aagagcatcg caataccgtg ctcgtcggcg gcggcaatca cttcgtcatc
    28141 acggatggaa ccgccaggct ggattacgca ggtcacgccc gcagcggcgg cggcatcaat
    28201 accgtcgcgg aacgggaaga acgcgtcaga agccatcgag gaacctttca cttccaggcc
    28261 ttcatcggca gctttaatac cggcgatttt cgcggagtac acgcggctca tctggcccgc
    28321 gccaataccg atggtcatat tgtttttggc atagacgata gcattggatt tcacgaattt
    28381 cgccactttc cagcagaaca gcgcatcacg cagttcctgt tcggtcggct gacgttgggt
    28441 gacgacgcgc agttcttccg caccgaccat ccccaggtca cgatcctgaa ccagcagacc
    28501 gccgttcacg cgtttgaaat caagacccgg aacacgctcg ccccactgac cgcaggtcag
    28561 aacgcgtacg ttctgcttgg cggcggtgat tttcagggct tcttcgctgg cggaaggcgc
    28621 aataatcact tcgacaaact gacgagaaat gatggcctgt gcggtttccg catccagctc
    28681 gcggttaaag gcaatgatgc cgccgaatgc ggaggttggg tcggttttgt acgcgcgatc
    28741 gtaagcatca agaatggaat tgccgatagc tacaccgcaa gggttggcgt gcttcacaat
    28801 cacacatgcc ggctcggcga actctttcac gcactccagc gccgcatcgg tatcggcgat
    28861 gttgttataa gagagggctt taccctgaac ctgggttgcg gtagcaacgg aggcttcttt
    28921 cacattctct tctatataga aggcagcctg ctggtggctg ttctcgccgt aacgcatatc
    28981 ctgcttctta atgaagttca ggttcagcgt gcgtgggaag cgaccggcgg cttctttgct
    29041 ttcaccgtgg tacgccggaa ccatgctgcc gaagtagttg gcaatcatgc tgtcgtaggc
    29101 ggcagtgtgt tcgaaggctt tgatggcgag gtcgaaacgg gttgcaagcg tcagcgatcc
    29161 ttcgttgtca tccatctctt taataatggc gtcgtagtcg ctgctcttca ccacgattgc
    29221 gacatcttta tggttcttag cggcggagcg caccatggtc gggccgccga tatcgatgtt
    29281 ctcaaccgca tcttccagcg agcagccttc acgagcaaca gtctgggcga acggatacag
    29341 gttaacaacc accatatcga taggctggat ctgatgttct tccataatgg catcgtcctg
    29401 gccgcgacgg cccagaatgc caccatgtac tttcggatgc agggtcttca cgcgtccatc
    29461 catcatctcc gggaaaccgg tgtaatcgga aacttcggtt accggcagac ctttttctgc
    29521 taacagacgg gcagtgcccc ctgtagacag cagctccaca ccgcgtgcgg aaagtgcctg
    29581 ggcgaattcg acgataccgg ctttgtcaga aacactgagc agagcgcggc ggactggacg
    29641 acgttgttgc atggtaaatc ccctgggttt gactattaca gagagcgtta gctgaatttt
    29701 tcgcgaaaaa ctcagctaac gcccctaacg gggcatcctt atttttcgcc cgcattgtaa
    29761 cgaaaacgtt tgcgcaacgc tcgcgaattt ttctctttca atggtgatca caattttgac
    29821 agtggttacc gtgggaaaaa tacagaaatt acattgatga ttgtggataa ctctgtgcgt
    29881 aaaaaggtat aaagcggggt tttgctgggg aatgcagcag tcagtcattt ttctgcaatt
    29941 tttctattgc ggcctgcgga gaactcccta taatgcgcct tcatcgacac ggcggatgtg
    30001 aatcacttca cacaaacagc cggttcggtt gaaga
//
LOCUS       DAJVQR010000043        29686 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVQR010000043 DAJVQR010000000
VERSION     DAJVQR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            58..852
                     /locus_tag="OQM52_002053"
     CDS             58..852
                     /locus_tag="OQM52_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1563912.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            874..2283
                     /locus_tag="OQM52_002054"
     CDS             874..2283
                     /locus_tag="OQM52_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1563913.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="OQM52_002055"
     CDS             2261..3040
                     /locus_tag="OQM52_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1563914.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="OQM52_002056"
     CDS             3037..3897
                     /locus_tag="OQM52_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1563915.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="OQM52_002057"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="OQM52_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="OQM52_002058"
     CDS             complement(4637..4897)
                     /locus_tag="OQM52_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1563916.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="OQM52_002059"
     CDS             complement(4888..6159)
                     /locus_tag="OQM52_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1563917.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="OQM52_002060"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="OQM52_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1563918.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="OQM52_002061"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="OQM52_002061"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1563919.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="OQM52_002062"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="OQM52_002062"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1563920.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="OQM52_002063"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="OQM52_002063"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1563921.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="OQM52_002064"
     CDS             11501..11653
                     /locus_tag="OQM52_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS1563922.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11847..12938
                     /locus_tag="OQM52_002065"
                     /pseudo
     CDS             11847..12938
                     /locus_tag="OQM52_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13084..14121)
                     /gene="iap"
                     /locus_tag="OQM52_002066"
     CDS             complement(13084..14121)
                     /gene="iap"
                     /locus_tag="OQM52_002066"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1563923.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14373..15281
                     /gene="cysD"
                     /locus_tag="OQM52_002067"
     CDS             14373..15281
                     /gene="cysD"
                     /locus_tag="OQM52_002067"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1563924.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15283..16710
                     /gene="cysN"
                     /locus_tag="OQM52_002068"
     CDS             15283..16710
                     /gene="cysN"
                     /locus_tag="OQM52_002068"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1563925.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16710..17315
                     /gene="cysC"
                     /locus_tag="OQM52_002069"
     CDS             16710..17315
                     /gene="cysC"
                     /locus_tag="OQM52_002069"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1563926.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17365..17688
                     /locus_tag="OQM52_002070"
     CDS             17365..17688
                     /locus_tag="OQM52_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1563927.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17882..18193
                     /gene="ftsB"
                     /locus_tag="OQM52_002071"
     CDS             17882..18193
                     /gene="ftsB"
                     /locus_tag="OQM52_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1563928.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18212..18922
                     /gene="ispD"
                     /locus_tag="OQM52_002072"
     CDS             18212..18922
                     /gene="ispD"
                     /locus_tag="OQM52_002072"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1563929.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18922..19401
                     /gene="ispF"
                     /locus_tag="OQM52_002073"
     CDS             18922..19401
                     /gene="ispF"
                     /locus_tag="OQM52_002073"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1563930.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19398..20447
                     /gene="truD"
                     /locus_tag="OQM52_002074"
     CDS             19398..20447
                     /gene="truD"
                     /locus_tag="OQM52_002074"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1563931.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20428..21189
                     /gene="surE"
                     /locus_tag="OQM52_002075"
     CDS             20428..21189
                     /gene="surE"
                     /locus_tag="OQM52_002075"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1563932.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21183..21809
                     /gene="pcm"
                     /locus_tag="OQM52_002076"
     CDS             21183..21809
                     /gene="pcm"
                     /locus_tag="OQM52_002076"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1563933.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21949..23088
                     /gene="nlpD"
                     /locus_tag="OQM52_002077"
     CDS             21949..23088
                     /gene="nlpD"
                     /locus_tag="OQM52_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1563934.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23151..24143
                     /gene="rpoS"
                     /locus_tag="OQM52_002078"
     CDS             23151..24143
                     /gene="rpoS"
                     /locus_tag="OQM52_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1563935.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24263..24670)
                     /locus_tag="OQM52_002079"
     CDS             complement(24263..24670)
                     /locus_tag="OQM52_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1563936.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24817..25410
                     /locus_tag="OQM52_002080"
     CDS             24817..25410
                     /locus_tag="OQM52_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCS1563937.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25410..26357
                     /locus_tag="OQM52_002081"
                     /pseudo
     CDS             25410..26357
                     /locus_tag="OQM52_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26630..27193)
                     /locus_tag="OQM52_002082"
     CDS             complement(26630..27193)
                     /locus_tag="OQM52_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563938.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27358..27609
                     /locus_tag="OQM52_002083"
     CDS             27358..27609
                     /locus_tag="OQM52_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1563939.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27611..>29686
                     /locus_tag="OQM52_002084"
     CDS             27611..>29686
                     /locus_tag="OQM52_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1563940.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQE"
BASE COUNT         7251 a         7366 c         8034 g         7035 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggagt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggca gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac ctgccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacattcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcctggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttta aatttatatt ttttatgtga atatatagtc tctatttatt
    11761 attatgaatt tcaaatgaaa atcattaatt tattcataat gtaaatgttt tattgactga
    11821 tatatatcat agatagtttt taaaatatgc gtaaatatcc tttaagttta ctgaaggata
    11881 aaaatattgt gactttcttt gatttctggg gaaaaaaccg acgtggcgag aaagagggta
    11941 tcgacgacta tcaccttctt tgctggcatt tgctggatgt ggccgcaatg ggctatttaa
    12001 tggttaaaag aaattgcttc gggctggctg attattttcg tcaattaggg atttctgaca
    12061 aggaacaggc agctcgattt ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg
    12121 cccgctcttt tcaacaactt tacctgcccc ctgaattcaa gattcaggaa ggtgccagaa
    12181 aaaattacga aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa
    12241 gtgaatgtca ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga
    12301 tagaaatgtg gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg
    12361 aattggataa ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca
    12421 agccactgtt tccgcgaata gagatcccca cattctggga tgatgacgag ggcgttgaac
    12481 ttataaaaca actttcctgg tatatctctg caaccgtcgt acttgcagac tggacagggt
    12541 cgtcaacgcg attttttcca cgcgtcgcac aggcaatgga tattgaagat tactggcaaa
    12601 aggctttagt tcaggctcaa aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc
    12661 ctttcaccgg aattaatacg ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa
    12721 aggtgctgga tctggatatc aatcagccag gaccacagtt gtttattctg gaagatgtga
    12781 ccggcgcagg taaaacagaa gcggcgctta tcctggcgca caggttgatg gctgcgggta
    12841 acgctcaggg gctgtttttt ggcctgccaa caatggcaac ggccaatgcc atgtacgatc
    12901 gactggtcaa aacctggctt gctttctatt cgccagaggt gttccccgct ccagcggaga
    12961 taaaccggcg caatacggtg cggttttccc ggaagtaaac cggacgcact ggatgcgatg
    13021 atgggtatca cttagaattc cccgcccctg cggtagaact cccagctccc attttcaaac
    13081 ccatcaagac gccttcgcca gctccttcac caacggtagc attatccgca taacgtcacg
    13141 gcagcgacgt tctattcttc caggaagtgc cttatcaata tgttgttgat tatccagtct
    13201 tacgtcatgc cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc
    13261 atccttattt ccaagattcc agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt
    13321 gtcgaatatt tctgcgtcat tacaaccccc agtgcctttc ggataatttt tattcaaacc
    13381 cggattggtc gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt
    13441 taatttcctt actgcttcag gggtttttac accgctgttg aaatacaatt tatcgccaac
    13501 aattaagtta tcgagattaa tcaccagcag cgtatttttc ttttcggtgt cactcatccg
    13561 cttgagtaaa ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg
    13621 aataccatac tcggtaggcg tatttttcag gcgttctgcc agttccagca tgacacctaa
    13681 acctgcggcg ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc
    13741 agcatcgctc agcggggcgt aagtatccag atgcgccata atgatgatct gctgcggcgc
    13801 tttgccttca tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt
    13861 atcgcgggcg gtataaatat accgactatt aaatgtccga atatcactgc gataacccat
    13921 ttgctgaaac tgttggcgaa tataatcggc agataacatt tctgctggag ttccggtcat
    13981 gcgtcccgga aagaaagtag caatatgtcg tgcctgggta ttagcaaaat cgccaggttt
    14041 aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg cagcggtacg
    14101 gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca
    14161 tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg
    14221 gtgccttaag cactttttga tattagcttt gccaaatcgt tattccgtta aggaactact
    14281 cattctaatt ggtaatttca ttcgttctct tacgctccct atagtcgaaa catctgatgg
    14341 caagaaaata gcggtattgc aaaggaacgg ttatggctca aaaacgactt actcacctgc
    14401 ggcaactgga ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc
    14461 cggtgatgct ctactctatc ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg
    14521 cgttttatcc aggtacgctg cctttcccgt tgctgcatgt cgataccggc tggaaattcc
    14581 gcgagatgta tgagttccgc gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc
    14641 ataaaaaccc ggaaggcgtg gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac
    14701 ataccgatat tatgaaaact gaaggcctga aacaggcgct gaacaaatac ggttttgatg
    14761 ccgccttcgg tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag cgaatctact
    14821 ctttccgtga ccgcttccat cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca
    14881 actacaacgg gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga
    14941 ccgagcagga tatctggcaa tacatctggc tggaaaatat cgacattgtg ccgctgtatc
    15001 tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt gatgatgatt gatgacaacc
    15061 gtatcgacct gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg
    15121 gctgctggcc gctgaccggt gcggtggagt caaatgcaca aacactgccg gaaatcatcg
    15181 aagagatgct ggtttccacc accagtgaac gtcagggtcg cgtgattgac cgcgaccagg
    15241 cgggatccat ggagctgaaa aaacgtcagg ggtattttta agatgaacac cgtacttgca
    15301 caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc
    15361 ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca aaagtaccct gattggtcgt
    15421 ctgctgcacg atacccgcca aatctacgaa gatcagctct catcgctgca taacgacagt
    15481 aagcgtcacg gaacccaggg cgaaaagctg gatctggctc tgctggtgga cggcctgcaa
    15541 gctgagcgcg aacagggcat cactattgat gtggcctacc gctatttctc taccgagaag
    15601 cgtaaattta ttatcgccga caccccaggg cacgagcagt acactcgcaa tatggcgact
    15661 ggcgcatcga catgtgaact ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat
    15721 caaacccgtc gtcacagttt tatctccaca ctgttgggga tcaaacatct ggtcgtggcg
    15781 atcaacaaaa tggatctggt ggattacagt gaagagacgt tcacccgtat tcgtgaagat
    15841 tatctgacct ttgccgggca gctgccgggt aatctggata tccgctttgt gccgctctcc
    15901 gcactggaag gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg
    15961 acactgctcg aagtgctgga aaccgtggag atccagcgag tggtggatgc ccagccaatg
    16021 cgcttcccgg tgcagtacgt taaccgccca aatctcgatt ttcgtggcta cgccggaatg
    16081 ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa
    16141 tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt tgccggagaa
    16201 gcgatcaccc tggtgctgac ggatgagatc gatatcagcc gtggtgatct gctgctggcg
    16261 gcagacgaag cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa
    16321 cagccgcttt ccccgggcca gagttatgac atcaaaattg ccggtaagaa gacgcgtgct
    16381 cgtgttgatg gcattcgtta tcaggttgat attaataacc ttacccaacg cgaagttgaa
    16441 aacctgccgc tgaacggcat cggcctggtg gatctcactt ttgacgagcc actggtgtta
    16501 gatcgctatc agcaaaatcc ggttaccggt gggctgattt ttatcgatcg cctgagcaat
    16561 gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct
    16621 gaattcagtg cattcgaact ggaattgaat gccctggttc gtcgccactt tccgcactgg
    16681 ggcgcgcgcg atttgctggg ggataaataa tggcgctgca tgacgaaaac gtcgtctggc
    16741 atagtcatcc ggtcactgtg caacaacgcg aactacacca cggtcatcgt ggtgtagtgc
    16801 tgtggtttac cggcctctcc gggtccggta aatcaacggt cgccggggcg ctggaggagg
    16861 cgttacataa actcagcgtc agtacgtatc tgctggatgg cgacaatgtt cgccacggat
    16921 tatgcagcga tctcggtttt agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg
    16981 aagtggcgaa tttgatggtt gaagccggac tggtggtgct gaccgcattt atctcgccac
    17041 accgcgccga acgccagatg gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt
    17101 ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc aaaaggctta tataagaaag
    17161 cgcgtgccgg tgaactgcgc aactttacgg gaatagattc cgtttacgaa gcgcctgaat
    17221 cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa tttggtacag caattattag
    17281 atctgttgag acagaacgat attatcagat cctgagacac caccgggttt ccatgcccgg
    17341 ttcgtcaaag taacaggatc aaatatgcgt aatagccata acattacact aacaaataac
    17401 gacagcctta ctgaggatga agaaactaca tggtcactgc ctggtgccgt ggtcggtttt
    17461 atctcctggt tatttgcgct ggcgatgccg atgttgattt atggctctaa cacgctgttc
    17521 ttctttctct acacctggcc tttctttctg gcactgatgc ctgtcgcggt agtggtgggg
    17581 attgcgctgc attcattgat ggacggaaag ctacgctaca gtattgtttt caccctggtg
    17641 acagtaggca ttatgtttgg cgcactgttt atgtggctac tgggctaatt tgtactttct
    17701 tgtctcctct gttcataatt caaaccgtaa ctaataatga gattatgttc tgcacgccct
    17761 gggtatacgt aacaatgggc aaatgtggta catttgcccg cgttgtcgcg gtatccccaa
    17821 cagaggatgt agagtcgtct tcggatgcat gggatgatga tgcagttttt cagggggcag
    17881 gatgggtaaa ctaacgctgc tgttgctggc tattctggtc tggctacagt attcgctgtg
    17941 gttcggtaag aacggtatac atgactatac ccgcgtcaat gatgatgtgg cggcactgca
    18001 agctacaaac gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct
    18061 caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc
    18121 gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca
    18181 aaacaatcga taaatcagcc cgggaattaa catggcaacc actcatttgg atgtttgcgc
    18241 cgtggttccg gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct
    18301 ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg
    18361 ggtgacacgt gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct
    18421 ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct
    18481 ggcaggtctg aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc
    18541 ttgtttgcat caggatgacc ttgcgcgatt gttggcgttg agcgaaacca gccgcacggg
    18601 agggatcctc gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc
    18661 cattgctcat accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc
    18721 tcgtgagctg ttacatgact gtctgacgcg cgctctaaat gaaggcgcta ccattaccga
    18781 cgaagcctcg gcgctggaat attgcggatt tcatccgcag ttggtcgaag gccgcgcgga
    18841 taacattaaa gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac
    18901 catccatcag gagaatacat aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc
    18961 ggtgaaggcc caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg
    19021 cattctgatg gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg
    19081 ctgggggata tcggcaaact gttcccggat accgatccgg catttaaagg tgccgacagc
    19141 cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac
    19201 gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc
    19261 gtgtttattg ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact
    19321 acggaaaaac ttggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta
    19381 ctcattaagg caacaaaatg attgagtttg ataatctcac ttacctccac ggtaaaccgc
    19441 aaggcaccgg gctgctgaaa gccaatccgg aagactttgt ggtggtggaa gatttgggct
    19501 ttgagcctga tggtgaaggt gagcatattc tggttagaat cctcaaaaac ggctgcaata
    19561 cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct
    19621 tcgctgggca aaaagacaaa catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg
    19681 gcaaggaaat gcccgatctg agcgcctttc aactggaagg ctgccaggtg ctggagtatg
    19741 cgcggcacaa gcgcaagctg cgtttagggg cgctgaaagg taacgccttt accctggttc
    19801 tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag
    19861 gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg
    19921 cgctgcgctg ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg agtttttggt
    19981 tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag
    20041 acgttaatca agttgttgac ggcgatgcgc tacaattagc cggacgtggt agctggtttg
    20101 tagcaaccac cgaagaactg gcggaattac agcgtcgcgt caacgataaa gagctgatga
    20161 taaccgccgc attgccgggc agtggcgaat ggggaactca gcgtgaagcg ctggcattcg
    20221 aacaagcagc tgtcgccgca gaaactgaat tacaagcttt actggtgcgc gaaaaagttg
    20281 aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt aagctggaac tggtgggatg
    20341 acgtcaccgt agagatccgt ttctggcttc cggcgggtag ttttgcaacc agcgttgtca
    20401 gggaacttat caacacaaca ggtgattatg cgcatattgc tgagtaatta tgacggggta
    20461 catgcacccg gtatacaaac gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg
    20521 gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc tgacactgga atcctccctg
    20581 cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt
    20641 gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt
    20701 aacgccgggc cgaatctggg ggatgatgtt atttattccg gtacggtagc cgccgcgatg
    20761 gaaggccgtc atttaggttt tccggcgctg gccgtctcgc ttgacgggca taaacattac
    20821 gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc
    20881 accggacgta ttcttaatat taacgttcag gatttaccct tggatcaaat caaaggtatt
    20941 cgcgtgacgc gctgcggtac acgacatccg gcagatcagg tgatcccgca gcaagatccg
    21001 cgcggcaata cgctgtactg gattggcccg ccgggcggta aatgtgatgc cggtccgggg
    21061 accgattttg ctgcggtaga tgagggctat gtctccatca cgccgctgca tgtggattta
    21121 actgcgcata gcgcgcaaga tgtggtttca gactggttaa acagcgtggg agttggcacg
    21181 caatggtaag cagacgcgta caagcacttc tggatcaatt acgtgcgcaa ggtattcagg
    21241 atgagcaggt gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt
    21301 ttgaacaaaa agcctgggac aatatcgcct tgccgatagg tcaggggcag acaatttcgc
    21361 agccgtatat ggtggcgcga atgaccgaat tactcgagct gacgccgcag tcgcgggtgc
    21421 tggaaattgg cactggttcg ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg
    21481 tttgctcggt tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc
    21541 ttgatttaca taatgtttca acccgtcatg gtgatggatg gcaaggttgg caggcacgtg
    21601 cgccgtttga cgctatcatt gttacggcgg caccgccgga aattccaact gcgctaatga
    21661 cgcagctgga cgaaggcggg attctcgtct tacccgtagg ggaggagcac cagtatttga
    21721 aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg
    21781 tccctttagt gaagggtgag ctggcttaaa acgtgaggaa atacctggat ttttcctggt
    21841 tattttgccg taggtcagcg tatcgtgaac atcttttcca gtgttcagta gggtgccttg
    21901 cacggtaatt atgtcactgg ttattaacca atttttcctg ggggataaat gagcgcggga
    21961 agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct atggctggca
    22021 ggctgttctg acacttcaaa tccaccggca ccggtcagct ccgttaatgg caatgcgcct
    22081 gcaaatacta attctggtat gttgattacg ccgccgccga aaatggggac gacgtctaca
    22141 gcgcagcaac cgcaaattca gccggtacag cagccacaaa ttcaggctac tcaacaaccg
    22201 caaatccagc cagtgcagcc agtagctcag cagccggtac agatggaaaa cggacgcatc
    22261 gtctataacc gtcagtatgg gaacattccg aaaggcagtt atagcggcag tacctatacc
    22321 gtgaaaaaag gcgacacact tttctatatc gcctggatta ctggcaacga tttccgtgac
    22381 cttgctcagc gcaacaatat tcaggcacca tacgcgctga acgttggtca gaccttgcag
    22441 gtgggtaatg cttccggtac gccaatcact ggcggaaatg ccattaccca ggccgacgca
    22501 gcagagcaag gagttgtgat caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa
    22561 ccgacaatta cgtattctga gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac
    22621 aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc aagcacaacc
    22681 gagccgactg tcagcagtac atcaaccagt acgcctatct ccacctggcg ctggccgact
    22741 gagggcaaag tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg gattgatatc
    22801 gcaggcagca aaggacaggc aattatcgcg accgcagatg gccgcgttgt ttatgctggt
    22861 aacgcgctgc gcggctacgg taatctgatt atcatcaaac ataatgatga ttacctgagt
    22921 gcctacgccc ataacgacac aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa
    22981 aaaatagcaa ccatgggtag caccggaacc agttcaacac gcttgcattt tgaaattcgt
    23041 tacaagggga aatccgtaaa cccgctgcgt tatttgccgc agcgataaat cggcggaacc
    23101 aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt atgagtcaga
    23161 atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg
    23221 tttttgacga aaaggcctta gtagaagagg aacccagtga taacgatttg gccgaagagg
    23281 aactgttatc gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg
    23341 agattggtta ttcaccactg ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac
    23401 tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag taacttgcgt ctggtggtaa
    23461 aaattgcccg ccgttatggc aatcgtggtc tggcgttgct ggaccttatc gaagagggca
    23521 acttggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc cgcttctcaa
    23581 catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta
    23641 ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg
    23701 agttgtccca taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata
    23761 agccagttga tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc tcggtagaca
    23821 ccccgctggg tggtgattcc gaaaaagcgt tgctggacat cctggccgat gaaaaagaga
    23881 acggtccgga agataccacg caagatgacg atatgaagca gagcatcgtc aaatggctgt
    23941 tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg attcggtttg ctggggtatg
    24001 aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc
    24061 agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata
    24121 tcgaagcgct gttccgcaag taagtaagca tctgtcagaa aggccagtct caagcgaggc
    24181 tggccttttt cttttgtttg ggaagcttgt gtttttgtcg cctggataag acacgtcagc
    24241 gtcctacccg gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca
    24301 gctcctgctc ttctccactc aaacgcccca aaaattcctc atccacgctg tcaccaatag
    24361 gtatcgccgc agcaagtagc ttttctcctt cggcagtcag ccagacaaag cgtcgccgct
    24421 tgtcagcgac gtcatgttca cgtctgacta agccacgatt ttccattctt gccaacattt
    24481 ctgctagcgt tgctttggtg ctgactgctg cttctatcag tgccacctgt tcaataccag
    24541 gtttatcagc aatggcgcgc atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt
    24601 gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc
    24661 gtaacgccat gtaaactccg ggctaattct ctgtcgctat catagcggtt ttttagtgac
    24721 gaagaagatg ttgttacttt tcaatccttg ccgctgtgga aatgaaggag tatgttaata
    24781 ataacgttcg tatacgaata attaagagaa taagcaatga aactgatcgt cgggatgaca
    24841 ggggctaccg gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga gatgccgaat
    24901 gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct
    24961 tacagcgctc gcgatgttgc tgccctcgca gacttcagcc ataacccggc ggatcaggcg
    25021 gcgatcatct catccggttc ttttcgtacc gacggcatga tcgttattcc gtgcagtatg
    25081 aaaacgctcg ccggtatccg cgctggttac gccgatggcc tggtagggcg cgcggcggac
    25141 gtcgtgctca aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc
    25201 atccatctcg aaaatatgct cgcactttca cgcatgggcg tggcgatggt gccgccgatg
    25261 cctgcctttt ataaccatcc cgaaacggta gatgacattg tccaccatgt ggtagcccgc
    25321 gtgctggatc aatttggcct cgaacatccc cacgccaggc gctggcaagg attgccgcag
    25381 gcccggaatt tttctcagga gaatgaataa tggcatttga tgatttacgc agctttttac
    25441 aggcgcttga tgaccacggc cagttactga aaatcagcga agaagtgaac gctgagccgg
    25501 atctggcagc agcagctaac gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt
    25561 ttgataatat tcgcggcttt accgatgccc gcgtggcgat gaataccatc ggttcctggc
    25621 agaaccacgc gatttccctt ggcctgccac caaacacccc ggtcaaaaag cagattgatg
    25681 agtttatccg ccgctgggat aacttcccga ttgccccgga gcgccgcgcc aatccagcct
    25741 gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc
    25801 gcctgaatga tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc
    25861 cgctcgaccc ggataacttc ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg
    25921 gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga tatcgccctg catctgcata
    25981 aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac gatccgatca
    26041 tcacgctgat gggggccacg ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg
    26101 cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt
    26161 ggggttcaga agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc
    26221 cgttcggtga gtttaccggg cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg
    26281 ataaagtctc ttatcgcagc aaaccgattt tcgaatcact ctatctcggt atgccgtgga
    26341 ccgaaatcga ctacctatga ctaggtaatt tattcaagat aaccgggaag agattaaaaa
    26401 agacgggaat agattggaat caacaagtta ggaactcaag gttttcaaga taaagcgagt
    26461 ccaaaaatgt taaacggttc gatatcaata aaacattcaa gataactcac aggttcttac
    26521 aatagccttt aaagaccgtt caaaaacgat tcaggtgttt attatagcgc tgatgggtgc
    26581 aagcgccaaa accaatgtaa ttttagcaca gctaggcact tgcactgggt tatgcgctgg
    26641 ctgctttctg aggtagattt aagtgatcgc ccttagcttc ggacaacgcg taaatctctc
    26701 taaccttttc gtctggcaaa ttaccaaggc ttaacgctat ggctctacga actccaacta
    26761 gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag taattgctcc gctccttttc
    26821 tcccaagaac atcagctact tgttgttttt cttgtggttt aagaaatgac aagaacatta
    26881 gaatttgagc atcaacatta tccgcattct cttttttggg ttctttggaa tcttttagtt
    26941 caacatcacc ctctcccgtc atgagccatt ccaaggtaac attttcgctt tctgctatct
    27001 tttttaactg atacgggcgt ggtatcggtg cgtaatcccg cctgttgtaa taatttttta
    27061 aagtgccttc attgatcccc catgcccgag cagccgcatt tcttgaaggg tatcgttcta
    27121 ctaacctaaa gattctttcg ataatcggtt cttttccatc ggcaggaaaa gaaccttggg
    27181 gtgcttttct cattcttggt tcctttctat ctaattcatt gataaataat aaaattaaaa
    27241 aataaaccat aaaaacacca aagaaccctt tgcagggaat ctttggtatc ttaatcttta
    27301 tccatacgga tatccgaccg gatatccgcg cgggtaattt tgtaggttaa aggaaagatg
    27361 agacaaagag aagatagtga ccgcgattgg ccttcgctga agattcagct ggcgctcaaa
    27421 gataacgggt ggtcttcctt ccgggagctt tcactccatg ctggttatgc tcaggattca
    27481 ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac gcgtaatttc tgacgctata
    27541 ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta acgatggatt tatcaggaag
    27601 gcgtcgtgat atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac caggaatgcc
    27661 agccaccaca aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt
    27721 tagtcgccgt cgggaaggca caaaagccat tgagtaccac attgattgtc tgcctgaaat
    27781 aacacgaaag gcactgcgtg aacgctatgt ggaacagctg gtggctacag agaacaatgt
    27841 ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga
    27901 agcatatcgc ggttctccac aactgatgga agaacgcctg aatgcgctga ccgaaaacca
    27961 gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc
    28021 cggtaacccc gggcgactga aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga
    28081 gctgccggag cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg
    28141 tactatcagc cgcgatccac tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc
    28201 cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac gaaataaaac cggaagaaat
    28261 cagctggctg cctgaatttc tggcgcagta tcgccaggtg aacggcagac ccatgtcaga
    28321 agcctacgag gattttgtcg cagaatggca gcgtcgccat gcggacgaac cgtacatgct
    28381 ggaggtgatg ccttcctatg atgttgtccg gtatgccatg aaaaaactgc cggaagtggt
    28441 gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag cttgaaggat tcacccgccg
    28501 cgactggaca gctatgccgg tgaattatgt ctggattggt gacggtcacg gtatgaagct
    28561 gaaatgtgcg catccgatcc acgggcgtcc attctcaccg gaagtcacgt ttgtgatcga
    28621 tggtggaaca cggtttgttg ttggctggag tcttgacctt gccgaaaacg tgttcgccgt
    28681 tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa ccgttcctgt attactcgga
    28741 taacggttcc ggtgaaacgg ctgacatgct ggacaaagag gtcgtgggta ttctgccacg
    28801 cctggggatt aaacacccta ccggtattgc cggtaatcca cagggacgcg gcattattga
    28861 acgcctgaac cggacactgc cgatgcgtat agccaggagg tatcgcacct attttggcaa
    28921 gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat ctgcgttcag cgtttaacgc
    28981 cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg gcaatgcgcg agctgccatc
    29041 gtgggcggag cttattgagg ctattcgtga aggcgtggaa tggtacaaca accgcccgca
    29101 ttctgagctg ccgatgaaac cgaacggacg gcattacagc ccgacagagt tcagaaaaaa
    29161 acgccaggca gaagaagaca ccgaaattga gtggctgagt gatcttgagc tgcgcgacat
    29221 gttccgccca atggtggaac gaccggtaag acgctgcgaa attcagtggc tgaacaatat
    29281 ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa gtgcttatca gttatgacat
    29341 tcatgacgcc gagcgaatta cggtacgtcg caaggacggc agctttatct gtgaggcgat
    29401 atggaacggc aataaacgcg cagcctttgc tgtcagtgct gaatatcaca aacaacagca
    29461 gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt cgtgatgctg aagatgaagg
    29521 tattcagatt ctggaacaca aacaggctga accctggctg agtaatgttt atcgcccggt
    29581 tggtaatgtg gtggcagttc agcaaccgga atatgaagaa gagcatgacg aagaatttga
    29641 acgcgatttc cggctgggga tgcaaaaact ttttgccatg caggaa
//
LOCUS       DAJVQR010000044        29394 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVQR010000044 DAJVQR010000000
VERSION     DAJVQR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(1..439)
                     /locus_tag="OQM52_002085"
                     /pseudo
     CDS             complement(1..439)
                     /locus_tag="OQM52_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(414..1214)
                     /gene="otsB"
                     /locus_tag="OQM52_002086"
     CDS             complement(414..1214)
                     /gene="otsB"
                     /locus_tag="OQM52_002086"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS1563941.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1381..2367)
                     /gene="araH"
                     /locus_tag="OQM52_002087"
     CDS             complement(1381..2367)
                     /gene="araH"
                     /locus_tag="OQM52_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1563942.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2382..3896)
                     /gene="araG"
                     /locus_tag="OQM52_002088"
     CDS             complement(2382..3896)
                     /gene="araG"
                     /locus_tag="OQM52_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1563943.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3966..4955)
                     /gene="araF"
                     /locus_tag="OQM52_002089"
                     /pseudo
     CDS             complement(3966..4955)
                     /gene="araF"
                     /locus_tag="OQM52_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5752..6255
                     /locus_tag="OQM52_002090"
     CDS             5752..6255
                     /locus_tag="OQM52_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1563944.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6335..6586)
                     /locus_tag="OQM52_002091"
     CDS             complement(6335..6586)
                     /locus_tag="OQM52_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1563945.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6701..6787)
                     /gene="azuC"
                     /locus_tag="OQM52_002092"
     CDS             complement(6701..6787)
                     /gene="azuC"
                     /locus_tag="OQM52_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1563946.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7050..7373
                     /locus_tag="OQM52_002093"
     CDS             7050..7373
                     /locus_tag="OQM52_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1563947.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7544..8041
                     /gene="ftnA"
                     /locus_tag="OQM52_002094"
     CDS             7544..8041
                     /gene="ftnA"
                     /locus_tag="OQM52_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1563948.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8078..8317)
                     /locus_tag="OQM52_002095"
     CDS             complement(8078..8317)
                     /locus_tag="OQM52_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1563949.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8507..9718
                     /gene="tyrP"
                     /locus_tag="OQM52_002096"
     CDS             8507..9718
                     /gene="tyrP"
                     /locus_tag="OQM52_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1563950.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9780..10445)
                     /gene="yecA"
                     /locus_tag="OQM52_002097"
     CDS             complement(9780..10445)
                     /gene="yecA"
                     /locus_tag="OQM52_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1563951.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10641..10727)
                     /locus_tag="OQM52_002098"
     tRNA            complement(10641..10727)
                     /locus_tag="OQM52_002098"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10691..10693),aa:Leu,seq:taa)
     gene            complement(10740..10813)
                     /locus_tag="OQM52_002099"
     tRNA            complement(10740..10813)
                     /locus_tag="OQM52_002099"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10779..10781),aa:Cys,seq:gca)
     gene            complement(10868..10943)
                     /locus_tag="OQM52_002100"
     tRNA            complement(10868..10943)
                     /locus_tag="OQM52_002100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10908..10910),aa:Gly,seq:gcc)
     gene            complement(11095..11643)
                     /gene="pgsA"
                     /locus_tag="OQM52_002101"
     CDS             complement(11095..11643)
                     /gene="pgsA"
                     /locus_tag="OQM52_002101"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1563952.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11700..13532)
                     /gene="uvrC"
                     /locus_tag="OQM52_002102"
     CDS             complement(11700..13532)
                     /gene="uvrC"
                     /locus_tag="OQM52_002102"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1563953.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13529..14185)
                     /gene="uvrY"
                     /locus_tag="OQM52_002103"
     CDS             complement(13529..14185)
                     /gene="uvrY"
                     /locus_tag="OQM52_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1563954.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14481..14657
                     /locus_tag="OQM52_002104"
     CDS             14481..14657
                     /locus_tag="OQM52_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1563955.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14644..14868
                     /locus_tag="OQM52_002105"
     CDS             14644..14868
                     /locus_tag="OQM52_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1563956.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14901..15521)
                     /gene="sdiA"
                     /locus_tag="OQM52_002106"
                     /pseudo
     CDS             complement(14901..15521)
                     /gene="sdiA"
                     /locus_tag="OQM52_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15751..16503)
                     /gene="yecC"
                     /locus_tag="OQM52_002107"
     CDS             complement(15751..16503)
                     /gene="yecC"
                     /locus_tag="OQM52_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1563957.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16500..17168)
                     /gene="tcyL"
                     /locus_tag="OQM52_002108"
     CDS             complement(16500..17168)
                     /gene="tcyL"
                     /locus_tag="OQM52_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1563958.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17183..18169)
                     /gene="dcyD"
                     /locus_tag="OQM52_002109"
     CDS             complement(17183..18169)
                     /gene="dcyD"
                     /locus_tag="OQM52_002109"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1563959.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18274..19074)
                     /gene="tcyJ"
                     /locus_tag="OQM52_002110"
     CDS             complement(18274..19074)
                     /gene="tcyJ"
                     /locus_tag="OQM52_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1563960.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19162..19713)
                     /gene="fliZ"
                     /locus_tag="OQM52_002111"
     CDS             complement(19162..19713)
                     /gene="fliZ"
                     /locus_tag="OQM52_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1563961.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19759..20478)
                     /gene="fliA"
                     /locus_tag="OQM52_002112"
                     /pseudo
     CDS             complement(19759..20478)
                     /gene="fliA"
                     /locus_tag="OQM52_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20643..22295)
                     /gene="fliC"
                     /locus_tag="OQM52_002113"
     CDS             complement(20643..22295)
                     /gene="fliC"
                     /locus_tag="OQM52_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1563962.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22561..23967
                     /gene="fliD"
                     /locus_tag="OQM52_002114"
     CDS             22561..23967
                     /gene="fliD"
                     /locus_tag="OQM52_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1563963.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23992..24402
                     /gene="fliS"
                     /locus_tag="OQM52_002115"
     CDS             23992..24402
                     /gene="fliS"
                     /locus_tag="OQM52_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1563964.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24402..24767
                     /gene="fliT"
                     /locus_tag="OQM52_002116"
     CDS             24402..24767
                     /gene="fliT"
                     /locus_tag="OQM52_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS1563965.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24845..26332
                     /gene="amyA"
                     /locus_tag="OQM52_002117"
     CDS             24845..26332
                     /gene="amyA"
                     /locus_tag="OQM52_002117"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1563966.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26366..26779)
                     /locus_tag="OQM52_002118"
     CDS             complement(26366..26779)
                     /locus_tag="OQM52_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1563967.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26966..28171
                     /gene="yedE"
                     /locus_tag="OQM52_002119"
     CDS             26966..28171
                     /gene="yedE"
                     /locus_tag="OQM52_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1563968.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28168..28401
                     /gene="yedF"
                     /locus_tag="OQM52_002120"
     CDS             28168..28401
                     /gene="yedF"
                     /locus_tag="OQM52_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1563969.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28508..29178
                     /locus_tag="OQM52_002121"
                     /pseudo
     CDS             28508..29178
                     /locus_tag="OQM52_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29218..>29394)
                     /locus_tag="OQM52_002122"
     CDS             complement(29218..>29394)
                     /locus_tag="OQM52_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1563970.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
BASE COUNT         7319 a         7586 c         6997 g         7492 t
ORIGIN      
        1 atgcgattat tcaccccacg tttgcgtaat tcatgcgcaa agggcaacag gtgataatcg
       61 tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc cagcaacgca
      121 tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc gagccgataa
      181 tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc aaggtcctgt
      241 tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tagcggctga tcctcattcc
      301 ctgtttcacc actccagcca aaccacagtc cgcctgcggc tttcagtgcc cccagtatgc
      361 caacggcaag gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc cggttagata
      421 ctacgactaa acgactcata gtcatcactc ctgttatttt ctcttttttg ttgtaatgcg
      481 gcggttatca tttcaagcca gctccagaca tccggcacac ccgccagtcg ccatgatgcc
      541 tgagttgcac ctgtgccaat ttttactgac attccgccca gtcggttaac gactgcgaag
      601 ccagattcat cggttaaatc atcgcccaga aatacgggcg ttcgcccgat aaaaggagct
      661 tcctgcataa aggctgcaat cgcttcacct ttactggtac ctctcggttt gatctcgaca
      721 acacactttc cctgctgtaa cgccatttgt ggccagatct gagtaatacg ttgcgctaat
      781 gtcattaatg cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa agccatccct
      841 ttcgcctcca gctccgcgcc gggatactga gcgatgaccg tatgcagttg cacgctaata
      901 tcacgcgcaa tcgcatccgg cagatgaacg atatgtgttt taccattgat gtcacggcgc
      961 tccgccccat gcacgcccgc taacgggaag cgataaggtt ttgccagtgc gtcaagctcc
     1021 accattgagc gccctgatat caatgccaat gcaccatcac ttgcggttgc cagtagctgt
     1081 agtccttgca gaatattgtc aggcacgacg acctgatcgg gatgcggttt gatttccgcc
     1141 agcgttccat caagatcaaa aaaccaggca tatttcgcgg atagttcagg ggtttcggtt
     1201 aacggttctg tcacccggct ctcctccttc tcttttctta ttgtgttgcg cagaccgcgc
     1261 tttacagaca tgtaagtata gacagtgtga cggaggtcgc catttgcaac gaagaacagc
     1321 caaccgttgt ggcggctggc tgtggtggga aaaaacgtta agttgttgtg gaaaaaagca
     1381 tcagacagtg cgcttcgctt tttgcttgta acggtcgaag atcaccgctg ccagcaggat
     1441 taagccgcga accacgtact gcgcgaaagg agaaatatta agcaggttca tggcgttttc
     1501 cacggtgcct aaaattaaga tacccgccac cacatatgag atttttccga tgccaccttt
     1561 cagagaaacg ccacctaaaa cgcaggcgga gataacaata agctcataac caatcgacgt
     1621 cattggctgc ccactagtca tacgtgaagc cagaataatt ccggctatcg cagataccag
     1681 gccagagaga acaaagataa taattttggt gcgaacaacc ggtacacccg ccagacgcgc
     1741 agcctcttcg ttcccgccaa tcgccagggt attacgacca aaggtggttt tattcagcag
     1801 caaaccaaag ataatcagac acgcgacggt gagccagatt ggcgcaggca gaccgaacca
     1861 gttagcgtaa ccaagggcaa agaagctttc atcttcgata ccgaccgctt taccgtctga
     1921 aatgatgtac gccagacctc gaacaatctg catcgttgcc agtgtcgtga tcagagcatt
     1981 tattttcagt ttggcgataa caaagccatt gaccaggcca cagagaacgc ccagcaacaa
     2041 acccgctgcc acgccaatcc acaggctttc agtcaggttg ataaccaccg ccgtggtgac
     2101 acccgcacag gcaattacgg aggcgacaga aaggtcaaag tcaccggaag caaggcagaa
     2161 caacatgcca cacgccacca tccccgacat ggaaattgcc aggcccaacc ctttcatatt
     2221 aatgaaggtg gcaaaatttg ggacaaaaat ggcacaggcg ataaagagca ccgcaaacac
     2281 caccagcatg ccgtactgat cccagatacg cccgaagctg aatgacgact taggtgcgcc
     2341 agaccccgat gtagaaacag aagacatcat actctcctta ctcaggcaac tgcctggctg
     2401 actttaggca tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg taacaattca
     2461 ccggcgattt cgccttcccg catcaccaca atccggtcgg caacgccgag gacttcaggt
     2521 aagtcgctgg aggcaaacag caccgccacg ccctgcgccg ccagcgcata aattacgtta
     2581 tatatttcgt gcttagcgcc aacatcaatg ccgcgcgtag gttcatccag caaaatgacc
     2641 ttcatctctt ccgataacca gcggcccaga atggcttttt gctgatttcc gcctgagaga
     2701 ttcatgatca gttgctccgc gcccggcgtt ttgatgttga gcgaacgaat gtgctgatcg
     2761 gcattgtttt cttcccaact gttgttgatt acacaaccgc cgagcacatg tttgcgtctg
     2821 gcactgatgt tgatattgtc gcgaacggag tgcacgggaa taatgccttc cgctttgcga
     2881 gcttccgggc agagcatcat gcctgcggca atggcgtggc tcggtttacg aatatcgatc
     2941 ggctgttggt cgatataaac ctgaccggcg gtgatttgcg tcccgccaaa caagcctttc
     3001 attaattcgc tacgccccgc ccctaccaga ccaaacagcc caacaatttc accactgcga
     3061 accgccagac ttattggcgt acgcacgcct agtgctttca cagcatcaag acgcaggcgc
     3121 tcctcgccat aactacgagg ttgccagccg tagatatcgc caatgtcgcg cccgaccatc
     3181 gcctgcacca gcgcgtcgtg gtcaacctgc tgcatatcgg taaaggtttt gacataacgt
     3241 ccatctttaa agacagtgat ggcatcgctg agggcaaata tttcttccat acggtgagaa
     3301 acgtataaga tcacccgccc ctcttttcgc agttcacgaa taacgcggaa aagattgccg
     3361 atttcgcggg cagaaaggga gctggttggc tcatcaaagg cgataatttt agcgttacgc
     3421 gccagcgctt tggcgatttc aaccatctgc cactgaccaa tggcgagata tttcagcggc
     3481 gtgtccgggt caatatccat accaagatgt ttaagttgta aacccgcctc ataattcagc
     3541 aatgagcgat tcacaatgcc gcctttatgc ggcagctggc cgagatagat gttttccgcg
     3601 acggtcattt ccggcacgag atgcagttcc tggtaaataa tcgccacacc cgcgttaagt
     3661 gctgcggtcg tgtcggaaaa ggacatttcc tgcccattaa tcactacaga acccgtggtt
     3721 ggcgcatagt tgccgctgag gatttttaag agagttgatt ttcctgcgcc attttcaccc
     3781 atcaacgcat gaacctgacc ggcatagcag tcaaaactaa tatccgtcag cgccttaacg
     3841 ccgggaaacg ttttaccgat gccgcgaaat gagagatacg gggtagactg ttgcataacg
     3901 tctccgtgaa tcactggtcg ttcacacccc ctctgcatca tgcagagggg aatttttccg
     3961 gcaaattact taccgcctaa accttttttc tccagttctt ctttaaagtt gtcacgcgtg
     4021 atcagtacca cgtctgtaac ttcggtaaat tttggcgttt caacgtcttt tgctacccag
     4081 ttgtaaagca tttcgctgga tttatagcca tgtacgtccg ggcttggcag cagggaaccg
     4141 tagaagccgg ttgcctgtgc tttagacagt tcgcttactg catccacacc gttaatacca
     4201 ataccgatga tatcggctgc tttaaagccc tgaccttcgg ttgcacgtac gccgccaagt
     4261 acggtgctgt cgttcatacc gacgatcagc caatgtttaa cttccggatg ttgaaccagc
     4321 attgagttgg cagcgtcaaa tgcccccggg atgtcgttag atttggtagg tacctgataa
     4381 atttgttttt ccgggaatcc tgcagctttc agtgcgtcca tagaacccgt tgtacggcgg
     4441 cgggcggtat ccagttcgtt ggcggtaatc gccatcactg cgctttcttt gacatcccag
     4501 ccacgtttct gcatctcttt atacagttcc tagccctgac gttcgccaat tttagtcgcc
     4561 gccatcatca ccagaggaac ggtatccatt ggcttacctt tggcgttaac aaactggtca
     4621 tccactgcaa tgactttcat atcgtagcca cgcgctttcg cgacgatggc agagccgagt
     4681 ttcgggtccg gagtacaaat aacgaaacct tttgcgccac tggctgccag gctgtcgatc
     4741 gcgttcaatg ttttttcgcc atccggcacg gcaatcttaa taacctcaaa ccctaaatcc
     4801 ttcccggctt tatcggcaaa cttccattcg gtctggaacc acggctcttc cggttgcttc
     4861 accagaaaac cgagcttcag gttctccgcc atagcggatt gtgacataac ggctgccaga
     4921 ccaatggctg ccagggcttt agtaaatttg tgcatggttc tctccagctt tagtgtcgtt
     4981 ttgtgtaggg caaaaacgaa tgacattcgt taaattaatc ggaaaacaaa gcattacctt
     5041 ttatctaaaa gataagtgac cgtgttgaca tagttttagc gagaaattaa ttctccatag
     5101 gagagcaata tcacatcgca gaattacagt gagaacgtgc ataaatttag cgggaaaaga
     5161 cataaggaaa agccaatttg tcagacaaat tgttgaatgc acagcagatt aatccataag
     5221 attagcctgg aaatccttgt tgtcttcggt acccatgcgg gatgtcttct ttttaaccag
     5281 tcaataggcc gcattacctg gcgttgagtt tttgaaatgg tgtaataacc acaactcaaa
     5341 gatgtggaaa atgcgcgtca ttcatttcgc cattaattat cactgtgctc attaattaac
     5401 agaacacgta taatgagagc catctcgcaa aaatgaaaaa acgttttata aaatcatcac
     5461 ttcatcatga attcaaattc atcgattaat atcaacaaga tacaaaaagc actatcatta
     5521 aaattcattg catttacatt gatttcacca atgaaatgta aaaatatata aacttgatga
     5581 tttaagcatt ttcttataac cgttcagacg ttattcttat ttcagatcat cgtcagaatt
     5641 gactccacga tcacatttcg gaccggcaga aaggaattat tctgcaaaca gtaattatgg
     5701 tgttttgatt tatcttgcac ctctccactt ctggatataa ggatattagg tatggcaacc
     5761 gctggaatgc ttctcaaact caactctcaa atgaaccgcg agttttacgc atccaatctc
     5821 taccttcacc tgagtaactg gtgttctgaa cagagtctga acggcaccgc cactttcctt
     5881 cgcgcccagg cacagagtaa tgtgacccaa atgatgcgca tgtttaactt tatgaagagt
     5941 gtcggcgcta cccccatcgt taaagccatt gatgttccag gtgaaaaact gaactctctg
     6001 gaagaactgt tccaaaaaac gatggaagaa tacgagcaac gttcaagcac tctggcgcag
     6061 ttagccgatg aagcgaaaga actgaatgat gattcaaccg tcaatttcct gcgcgatctg
     6121 gaaaaagaac agcagcatga tggcctgttg ctgcaaacca ttcttgatga agtgcgcagt
     6181 gcgaaacttg cgggtatgtg ccctgtgcag accgaccaac atgttctgaa tgtcgtgtca
     6241 caccagctgc attgatcata catcggcgct aatacattgc gccgatgaag tttttgagaa
     6301 accgctgcct catctgtttg aagcagcggt ttttttaatg ggattcaccc tgtggggtaa
     6361 acttgagttc aatcagcgcg atggcttttt ggattgcccg catggtgacc gggtctgcgg
     6421 cagcgggatg atgagtaaag tcgatattct tcagctgact ggacattttt tcacgaactt
     6481 caacgggcgc gattacatcg agaacatcca gaatttgttt gataaccaac tggcaagcta
     6541 ccacatcaga aaccagttcc tgatcggcat tcagcggctg ggacatcgta aactcctgat
     6601 agcattttga aagccgttat agtagcggct tcacatcttc agcgatagtc acatccaccg
     6661 tcatcaggac acaaaaaaac ctgccggagc aggttttttg ttatcggaac atattgcctg
     6721 gcggtacgtc tttgaacgtc ttgcaatagt tattgaacat acttttcagg attttgcgca
     6781 gtttcatcgc ggcactccga ccatttgtta tacaggtgtt attgtctttg cgcttataat
     6841 atgacaacca tcacaaaaat caatctttat gtgatacaaa tcacataaat acccctttaa
     6901 tgttataaaa atgataatca aaaaacagcc cccctatttc tgacacctac agatggcaag
     6961 aaatagcgcc tgccaggcgt cttttccggc cattgtcgca gcgctgtaac gcgtaaaata
     7021 gtgctttctc ttactcttct ggctggacca tgagacttct gattctgact ctttcattaa
     7081 taacgctggc ggggtgtacg gttactcgcc aggcccatgt gagcgaagtt gacgcggcaa
     7141 caggtattgt acggttggtt tatgaccagg cttttttgca gcatgcccat accgatcgtt
     7201 atgtcagtcg cgggatcgcc gatcgcgcat gccagcagga aggctatacc cacgcgatcc
     7261 cctttggtca gccagtaggc aactgtagcc tttttgcggg ttccttatgt ttgaatacgg
     7321 aattcacttt gtcatatcag tgccatcatt ccgccttccc tgtctttctc taaatatttt
     7381 aatcagcgag gggatcttcg ctgattaaag aaatagcggt aatgcgtttt attcccattc
     7441 gtatttttaa taattgaagt ttatatttta ccttttgcaa ataataaaat aacaaattat
     7501 agtgacgcca cagcaacaaa tataaccttt gtggagcact atcatgctga aaccagaaat
     7561 gattgaaaaa cttaatgagc agatgaacct ggaactgtac tcttcactgc tttatcagca
     7621 aatgagcgcc tggtgcagct atcatacctt cgaaggtgct gccgcgttcc tgcgccgtca
     7681 cgcccaggaa gagatgacgc atatgcagcg tctgtttgat tacctgactg ataccggcaa
     7741 tttaccgcgt attaataccg ttgaatctcc gtttgctgaa tattcctcac ttgatgaatt
     7801 attccaggaa acctataaac acgaacaatt aatcacccag aaaattaacg aactggctca
     7861 tgctgcaatg accaatcagg actacccaac atttaatttc ctgcagtggt atgtttctga
     7921 gcagcatgaa gaagagaaac tgttcaaatc gattattgat aaattaagcc tggcaggcaa
     7981 aagcggcgaa ggtctgtatt ttatcgacaa agaactctct accctcgaca cacaaaacta
     8041 atgctctccg gcggcaggtt tctgccgccg tttcaaatca gtgacggcaa atcttactct
     8101 gatcggtcga aaaaccttct tctgaaggta taaatttgcc ttttgctgcc agaaacgcta
     8161 ccagctctcc cgctgtcacc ccttctgccg agcacgtgtg aaaacgtgcc tgttcaccaa
     8221 aacgcgcttt aatcgcagct tccagactgg catgcgtata ttgctcgcct gattcaatca
     8281 tcatatttaa cacttcatga ccgtgaatag agtccatcgt tcctcctcaa aaaaagccta
     8341 gcgtagcgat taccgcttat gaagactttg cgccagcgca ggactgaatg cttttattgt
     8401 acatttatat ttacacaata tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc
     8461 tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac
     8521 cctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc
     8581 gctggctgcg gccggtgttg gttttagcgt tacgttaatc ttgttgattg ggctttgggc
     8641 gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc cggcagatac
     8701 cggtctgggc acgctggcaa aacgctatct gggacgctac ggtcaatggc tgacgggctt
     8761 cagtatgatg ttcttaatgt atgctctgac tgcagcatac atcagcggtg ccggtgaatt
     8821 gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg ctggcgtgct
     8881 gttgttcact tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt
     8941 taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg tattgctgct
     9001 accgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc tggcactgtc
     9061 tgcaatcccg gtgattttta cgtcgtttgg ttttcacggt agcgtgccga gtattgtcag
     9121 ctatatggat ggcaacattc gtaagctacg ctgggtgttt ataatcggta gtgccatccc
     9181 cctggtggca tatattttct ggcaggtggc gacgcttggc agcattgatt caacaacctt
     9241 tatgggattg ctggctaatc atgctggatt aaacgggctg ttacaggcgt tacgcgaaat
     9301 ggtggcctct ccgcatgttg agctggcggt gcatttattt gctgatttag ccctcgccac
     9361 gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttgt ttcagcgttc
     9421 aaataccgtt ggtggacgat tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt
     9481 tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg tggcgctggc
     9541 ggtactggca ttgattatcc cttcactatt gacctggcaa agcagaaagc acaatcctca
     9601 ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat
     9661 tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag tggggtgatc
     9721 agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa tgtgacctct
     9781 taatgcaggc agcactgctt aaatttctta ccactgccgc acggacaagg atcgttacgc
     9841 cccggtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg atgcgccatc
     9901 cagtatgcat gtaaatcaag cgccgccagt cgaatggcat ctacgctctc ttcaaacgct
     9961 tctggcgaca ttttctctac ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc
    10021 tccagcgctg gttttaaaga atcgggcaac gttgaccagt cagaaagtgc cacgccccgc
    10081 atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag
    10141 ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt atcggccata
    10201 tgttgaaaag ccaggttcat aaagcgcgtc atctctttct ctgacgccca acgcggcaca
    10261 tagtcagccc caccccacac ggcaaccagc cactgttctg gttcaatctc ttgcggagaa
    10321 ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc gtggtcagtg
    10381 ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa cggtcccgtt
    10441 ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat gccggtccgt
    10501 atcagagata ctttttgagt ggctttgccg gtgattaaaa attaaggagg gtgtaacgac
    10561 aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt attactttga
    10621 ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac agcgcgaacg
    10681 ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg ggaagaaagt
    10741 ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta cataaccgct
    10801 ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgac gccgatgtct
    10861 tttaaactgg agcgggaaac gagactcgaa ctcgagacct cgaccttggc aaggtcgtgc
    10921 tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta
    10981 tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag
    11041 tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg
    11101 atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac
    11161 cgcagccaca aagaaaagtg caataccgac gtactcaacc caaatgttcg gacgccacag
    11221 cagccaggcc aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacggc
    11281 cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc
    11341 acgggcgatc atcgttgccg ccggtaatgt cacccaccag ctgtgataat gctcggttac
    11401 cagcaccatg gcgatagcca cgagaacttt atctgccaca gggtcaagga aagcaccaaa
    11461 ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc
    11521 gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa
    11581 tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg
    11641 cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat
    11701 caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt
    11761 gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac
    11821 atttgccgac gttttggccc gacgccttca atggtttcca gggaactggt atttttgacc
    11881 ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat gtgattcatc gcgaatatgc
    11941 tggataacat gcagcgcggg tgaatctggc ggcaaactaa atccctcacc ttccggctca
    12001 aagaacagcg tttccagtcc agccttacga tctgctcctt tggcaacgcc aagtagcagc
    12061 ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc
    12121 tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc aatggcttta
    12181 ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcct
    12241 gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag
    12301 gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact
    12361 tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc
    12421 gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc
    12481 ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa
    12541 tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg
    12601 cgcatctggc tgccttgtaa atagaactga cctacgaagg tttctaccac ctcgctcagt
    12661 tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga
    12721 atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcaagg
    12781 tcgtcgccgg tattggaaac aaactgtttt tcggtgacgc gtcgcaccgc ctgaatttgg
    12841 tcgcgaatac atgccgcttc ttcaaactcc agattctggc tggcagtttc catacggcta
    12901 atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc
    12961 tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct
    13021 atctggtatt gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga
    13081 atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac
    13141 gggccgaaat attcaccttt ggcatgcttt gcaccacgat gcatcgccag acgcgggtgg
    13201 gtatcgccac tcaggaagat aaaaggataa gatttatcat cgcgtagcaa aacgttgtaa
    13261 cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtatgc
    13321 gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg
    13381 aggttgctgc ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag
    13441 ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt tacggttttt
    13501 aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca
    13561 gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga
    13621 acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct
    13681 cattgacctt ctggccctta gtgatcatca gcataatctg caattcacgt tcagacaaac
    13741 tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga
    13801 tatcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct
    13861 gcggagccgc gcctttgctg aggtagccag cagcacctgc ctgcatgact ttcgctggta
    13921 aagggttttc tgtatggacc gtaagcatga tgattttaac atcagctgtg gaacgcgcga
    13981 ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt
    14041 caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc tctccgacga
    14101 cttttatacc cttaatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt
    14161 catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga
    14221 tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta aaaatggtgg
    14281 gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt
    14341 caccaacggc ttaccggatc gtttcacagt aagccctacg atgaaatgtg acaaaaattg
    14401 actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac
    14461 aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt aacgtatttt
    14521 atagcactgc cgatatcacg ctcaaaaaac aaccactgct atttttagca aagcttatgg
    14581 tatactccgc cgccttaaca tttttcacca caaattttca ttgcaacatg acgaggaaaa
    14641 taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc
    14701 tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc
    14761 cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca
    14821 gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc
    14881 tgagagcgtg cctttcagcc ggttattgac aaaatcatcg ctatctctgc tgatgtcttc
    14941 ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat ctctggcgtc
    15001 atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac cagtaactgc
    15061 atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgtgctgca acgggaaaag
    15121 gacaaaaagc ccagcgcccg atttggcagc attaaatact gagtgacacc gcggcgtaaa
    15181 ccatgtgcgt gcgcggcttc ccataatggc tgtgcttcgc tgaataagtc atcattccac
    15241 attaaatggc cctgactaaa gttttcaggg ttcagcacag gatcaattgc gagaaaattt
    15301 tttgcctgat aataactaac ccacgactca gggtaattgg tgtagaaagc cactttaggt
    15361 cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc cagctgctga
    15421 gcctgaagct ctatttcatg gtaggcctct tctgcggcct ccatcctctg aaaacgcaac
    15481 agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg caacgcccct
    15541 gagagtgagt atcatttata atgataattc aaactatcac ataaataaga tttatatata
    15601 atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct tatgttatta
    15661 atgagttatg ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc ccgccccgac
    15721 aggtgaatcg tcggggctga ttttttctta ttattgcagc agaagcttct cgaggaactg
    15781 gcgggtgcga ggctgctggg gatcggcaaa taacgctttt gctgccccct gctcgactat
    15841 ccgcccctgg tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg
    15901 tgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggatggtgt tcaggacttc
    15961 gcccaccagc tctggatcca gcgccgacgt tggctcgtca aacaaaatca cctcaggacg
    16021 cattgccagt gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca aacgacgtgg
    16081 atagctggtt tctttaccgg ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt
    16141 ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt tttccagcac
    16201 cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct gacgcaactg
    16261 gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatagtga tatcgccaac
    16321 ggtgatcgtt cccgcttccg gttgttccag cagatttatg ctacgtagca atgtggtttt
    16381 gccggaacca ctcggaccga taattgccac cacttcgcca ggctttacct cgaggtcgat
    16441 accgtgcagc accgtctgac cgtggaattt tttcaccagg ttcttaactt caatggcact
    16501 cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg caacgtcgat
    16561 aacacagtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt gaacacctcc
    16621 agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct ccggcacctg gattgtcgcg
    16681 gccagcgacg tatcttttac cagactgata aagctgttcg acagcggcgg caacgccacc
    16741 cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat accaatactg
    16801 gcagcggctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag cgtttcggcg
    16861 gcataggcgg cagtgttcag cgacagacca atcattgctg acggaatggg atctaattcg
    16921 ataccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag cggcgtacca
    16981 cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg cgacaggcgc
    17041 atcagcgcga gaataaaccc gagtagtaag ccaaaaaaca tgccgccgat actgagttgc
    17101 agggtatacc cagccccttt caacaggaac ggcaaggaat caataaccag ttgtatactt
    17161 tcttgcatga gcgttcccgg agctaaacgt ggggatgata ggcgaacagc gcaggcgcgc
    17221 cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga ctgataccgt
    17281 caatcagccc cgccatcgct tttccggtat acacaggatc aagcagaatg ccttccagac
    17341 gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa
    17401 aatagtcatc ccagagtaaa atttccgctg atgcggtcag ctccagttct ttcgcaatcg
    17461 cctgttgtag gttaaccact ttcggcaatt gatcggcaac ggaacgcgac acggtcacgc
    17521 caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccagtccg gcgtgagttc
    17581 cggcactgcc cgatgcgact accaccgacg aaatattaac cgccccttca cactgttgcg
    17641 cgatttccag cgcactctcc acataaccta gcgcgccaag agcattagaa ccgccaaccg
    17701 gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgccagttct tccagttggg
    17761 cattgggatc ggtcagtgcg tcgcacattt caatctgggt attgaacaga tccagcaaca
    17821 aacgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt tccagcagcg
    17881 ccacgcagtg cagaccgagt ttcgccgcga ctgcggcagt ctggcgcaca tggttagact
    17941 ggatcgcgcc ggcagtaatc agcgtatcgg caccttcgcg cagggcatct gccgcgagaa
    18001 attccagctt acgtaattta ttgccgccca ttgccatggg ggtgacctca tcccgtttga
    18061 tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc ggcgttggcg
    18121 cgccgataaa ctccaaccgt ggaaaacggg ttaaattatg cagtggcata acagcctccg
    18181 atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc
    18241 tagtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag caccaaacca
    18301 tttttcggaa agagcttgca gagtgccgtc tttttgcatt tccgcaattg catcattcac
    18361 cgctttcagc aggtcttcat ttcctttacg cagcgccacg ccagactcct gacgggagaa
    18421 tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc accagatcca gcgccgccag
    18481 acgatcaacg aggatcgcat cgatacgccc tacgcgcaga tcctgatatt tggtcgggtc
    18541 atcatcatag gtacgcacat cgacaccctg aacattctgc cgcagccact cttcatagtt
    18601 ggtgcccaga ccgacaccca cttttttgcc tttcagatca gcagccgttt taatggtgcc
    18661 ttcgttacct tttttcacca gcgcctgaat accggaaatg gtgtaagggg tagagaaatc
    18721 gtattttttc ttgcgctcgt cagaaatggt gacctgatta atcaccacat caatgcgttt
    18781 agagtccagc gacgccagca taccgtccca tttggtcggt tttagtgacg cctcaacgcc
    18841 aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc
    18901 cccctgaaaa ctgaacggcg gataagtgcc ttccagccct accagcagcg tgccgctctc
    18961 tttgacttta ttaagcagac cttcatctgc aaaactttta acgctcatgc ccgcaaccag
    19021 cgccacggcc atcacaccca tcaatgcctg acgtcccaga tgtgctaatt tcatattcac
    19081 cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca caaaaacaac
    19141 tccgctacat cttattctta tttaatatat atcagaagca ggcaggctgg aggatttctg
    19201 cacaagtccg gtacaggttt ggcgctgata gtgttgatac ttccgtaatg cgatgcggta
    19261 attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg
    19321 cagctgatcg agggaaatat tttgctcatg tagatgatta cccaaacggc gcagacgaac
    19381 gacatattca cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa atttcttaaa
    19441 gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc aaaatcggca
    19501 caatgccgcc catgattgtt gttccgtttg ccagcctttt tcatcaagca gcgtgtccag
    19561 gcgggaaatc tcgattttat tcactatttt gccgtcgcga actaaggtaa tgcgatcgag
    19621 taatttacgg caatgcgcgc aatgggtctg gctgtgttta aagtctttaa gatagcggct
    19681 taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt caaagttaaa
    19741 gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt taatgtcctg
    19801 gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa tctctttgag
    19861 attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg gcaacgtttc
    19921 gatggcttcc atcacccgct ggcgcagatt actgtccagc agttgttgta gcgggttttc
    19981 tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcac gccactcatc
    20041 gtaggagaag agctggctgt tattggtgtc gagcagcatt tgacgataat cggcaatatc
    20101 gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt cctgctccag
    20161 ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc
    20221 acggctgcga agttcatcca gcatagcgcc acggatacgc tacactgcgt aagttgtaaa
    20281 tgccgttcct tgtagggcgt catagcgttc gacagcatta agtaacccaa tgccgcccgc
    20341 ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc
    20401 gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta caccttcagc
    20461 ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg catgattatc
    20521 cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt atttgggggt
    20581 tacgcaaaaa gccgaatgta aaaaaccccg ccggtggcgg ggtttgagag gcattcagac
    20641 gattaaccct gcagcagaga cagaacctgc tgcggtacct ggttggcttt tgccagcacg
    20701 gagttaccgg cctgctggat aatctgcgct ttcgacatgt tggacacttc ggtcgcatag
    20761 tcggcgtcct gaatacggga ctgcgcttca gacaggttgg tagtggtgtt gttcaggttg
    20821 gtgactgcgg aatccaaacg gttttgcacc gcacccaggg aggagcggaa tttgtcgatg
    20881 gagctgatgg cttcatccag agctttcagg ggatcggctg tcgtagctgc ttttgtttca
    20941 gcatctgtcg tcaatttacc atcagcatct ttataaactg ccttaccgct gccgttagtc
    21001 actgaaccat catcctgtag gtaaaaatct acagtatcag atgtggtcaa cgcctcgttg
    21061 ttatcaacat aaaccgaatc aacacctttt gttacagcgc cagttgtgcc gttgacgcta
    21121 taacctgcag tagtgaaatc aacattgttt cccgaggttg atacagcttt atcgaaagct
    21181 gcctgatcaa cttttgcacc tgttactgaa taagtaatag tgtccggcgt agtactatta
    21241 ccggtaaagg agatagaggt accgccaatg tcaatggttg tcgctttggt attattggct
    21301 gcaagtgtgg acagactagc ctcagtcaaa gaagcaccag agccgttttt cgttaagcgc
    21361 ccagttgtat caatgtagag tttatctcca ttgcttgccg taattttacc atcactgctg
    21421 atctgaacat cctgtgcaga accatcgata gtaatagtgc ccttagcggt atcaccaact
    21481 cccggggtca aatatttctg aacatcggca gctgaagccg ttgtggtatc aaaagagtat
    21541 gacttacttg cactgtcata cttgtaattc gttgcactag ccgccgttcc gaagccgtta
    21601 ttaatggttg cctgaacagt atcaccatca ctaactccag ccagcaaatc agacgcttta
    21661 gcagcatcgt aacccgcact cacagtatat ttgttgccga ccactgttgc attagcagct
    21721 accaagtcag ctttagatgc agcagtatta gccacagccc cgccaccatt cacattaaac
    21781 ccattcagcc ccagcgtatc agagtcaatt ttcttcagat caatagtgat agtctggccg
    21841 tcattcgcac caacctgaat tttcatcgaa ccgtcttttg ccagtacgtt cacgccgttg
    21901 aactgggtct gaccggatac gcggtcaatt tcgtcgagac gggatttgat ttcgtcctga
    21961 atggagtcca gatccgaatc agagttagtc ccggtagaag cctgaaccgt cagctcacgg
    22021 atacgctgta agttgttgtt gatttcggac agcgcgcctt cagtggtctg tgcaacagaa
    22081 ataccgtcgt tggcgttacg tgcagcctga gtcaggcctt taatgttaga agtaaaacgg
    22141 ttagcaatcg cctgacccgc ggcgtcatcc ttcgcgctgt taatacgcaa gccagaagac
    22201 agacgctcga tagaactcga cagcgcagac tggttcttgt tgatattatt ttgagtgatc
    22261 agcgagaggc tgttggtatt aatgacttgt gccatgattc gttatcctat attgcaagtc
    22321 gttgattacg ttttgggttt ccacccgtcg gctcaatcgc cgtcaaccct gttatcgtct
    22381 gtcgtaaaac aacctttaga atttttttca caaacagcca ttttttgtta gtcgacgaaa
    22441 tactcttttc tctgcccctt attcccgcta ttaaaaaaaa caattaaacg taaactttgc
    22501 gcaattcagg ccgataaccc cggtattcgt tttacgtgtc gaaagataaa aggaaatcgc
    22561 atggcaagta tttcatcgct gggagtcggg tcaggtctgg atttaagttc aatccttgat
    22621 agcctcaccg ccgcgcaaaa agcgacgcta acccctattt caaatcagca atcgtcgttt
    22681 accgctaaac ttagcgccta cggtacgctg aaaagcgcgc tgacgacttt ccagaccgcc
    22741 aatactgcat tgtctaaagc cgatcttttt tccgccacca gcaccaccag cagcaccacc
    22801 gcgttcagtg ccaccaccgc gggtaacgcc atcgccggga aatacaccat cagcgtcacc
    22861 catctggcgc aggcgcaaac cctgaccacg cgcaccacca gagatgatac gaaaacggcg
    22921 atcgccacca gcgacagcaa actcaccatt caacaaggcg acgacaaaga tccgattacc
    22981 attgatatca gcgcggctaa ctcatcgtta agcgggatcc gtgatgccat caacaacgca
    23041 aaagcgggcg taagtgcgag catcattaac gtgggtaacg gtgaatatcg tctgtcagtc
    23101 acatcaaatg acaccggcct tgataatgcg atgacactct cggtcagcgg tgatgatgcg
    23161 ctacaaagtt ttatgggcta tgacgccagt gccagcagca acggtatgga ggtctcggtc
    23221 gccgcccaga atgcgcagct gacggtcaac aacgtcgcca tcgagaacag cagcaacact
    23281 atcagcgacg cgctggaaaa catcactctg aacctgaacg atgtcaccac gggcaaccag
    23341 acgctaacca tcactcagga cacctccaaa gtgcaaacgg cgattaaaga ctgggtgaat
    23401 gcctataact cgctaataga taccttcagc agcctgacca aatacaccgc cgtagatgcg
    23461 ggagctgata gccagagttc tagcaatggc gcactgctcg gcgactccac gctgcggacg
    23521 attcagacgc agttgaagtc gatgctgagt aataccgtca gttcttccag ctataaaacg
    23581 ctggcgcaga ttggtatcac gaccgatccc agcgatggca aactggaact ggatgccgac
    23641 aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag gtgcattgat tgttggcgat
    23701 ggtaaaaaaa ccggcatcac aaccaccatc ggcagcaacc tgaccagttg gctttcgaca
    23761 acgggcatta ttaaagccgc taccgatggc gttagtaaaa ccctgaataa gttaactaaa
    23821 gactacaacg ccgccagcga tcgcattgat gcgcaggtcg cgcgctacaa agaacaattt
    23881 acccaactgg acgttttaat gacctcgtta aacagcacca gcagctactt aacgcagcag
    23941 ttcgaaaaca acagtaattc caagtaatca ttattcatcg ggagacaggt catgtacgcg
    24001 gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa gcaccgtaat gagcgccagc
    24061 caacagcagt tggtcaccat gctatttgat ggagtgctga gcgcactggt tagagcgcga
    24121 ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa agcgatcaac
    24181 atcattgaga acggactgcg ggtgagtctt gatgaagaga gcaaagacga attaacccaa
    24241 aacttaattg ctctttatag ctatatggtc aggcgtttgc tgcaagccaa tttacgcaac
    24301 gatgtctcag cagtcgaaga ggtggaagca ttaatgcgta atattgccga tgcctggaaa
    24361 gagtctttac tctccccttc ttcgattcag gacccagtct gatgaacaat gcaccgcatt
    24421 tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc ctggcaacgg
    24481 aagaacaatg ggacgaactc atcgctagcg aaatggcgta tgtgaatgcg gtgcaggaga
    24541 tcgcacacct gactgaagag gttgccccgt ccactacgat gcaggagcag ctccgcccga
    24601 tgctgcacct gattctcgac aacgaaagca aggtaaagca gttattacag attcggatgg
    24661 atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta agtgcctatg
    24721 gcgatcaggg cggctttgtg ctggctccgc aggataacct cttttgaatc ttaatgagcc
    24781 gatggctcgc gaataatccg attacggcta cgcttctaat attccccttg aatggagtcg
    24841 aagaatgcgt aatcccacgc tgttacaatg ttttcactgg tattacccgg aaggcggtaa
    24901 gctctggcct gaactggccg agcgcgcaga cggttttaat gatattggta tcaatatggt
    24961 ctggttgccg cccgcctata aaggcgcatc gggcggctat tcggtcggct acgactccta
    25021 tgatttattt gatctaggtg agtttgatca gaaaggcagc atccctacta aatatggtga
    25081 taaagcacaa ctgctggccg ccattgacgc cctgaaacgt aatgacattg cggtgctgct
    25141 ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc gcgtgcagcg
    25201 tgtaaatgct gatgaccgta cgcaaattga cgaagaaatc attgagtgtg aaggctggac
    25261 gcgttacacc ttccccgctc gtgccgggca atactcgcag tttatctggg atttcaaatg
    25321 ttttagcggt atcgaccata tcgaacaccc tgatgaagat ggcattttta aaattgttaa
    25381 cgactatacc ggagaaggct ggaacgatca ggttgatgat gaactcggta atttcgatta
    25441 tctgatgggt gagaatatcg attttcgcaa tcatgccgtg acggaagaga ttaaatactg
    25501 ggcgcgctgg gtgatggaac aaacgcaatg cgacggcttt cgtcttgatg cggtcaaaca
    25561 tattccggca tggttttata aagagtggat cgaacacgta caggaagttg cgccaaagcc
    25621 gctgtttatt gtggctgaat gctggtcgca tgaagtcgat aaactgcaaa cgtatattga
    25681 tcaggtggaa ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat tccatgaagc
    25741 atcgcgcatg ggtcgcgact acgacatgac gcagatcttc actgggacgc tggtggaagc
    25801 cgatcctttt cacgccgtaa ccctcgttgc caaccacgac acccaaccgt tgcaagccct
    25861 cgaagcaccg gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt tgttgcggga
    25921 aaatggcgtt ccttcggtgt tctatccgga cctctacggt gcacattacg aagatgtcgg
    25981 tggtgacggg caaacctatc cgatagatat gccaataatc gaacagcttg atgagttaat
    26041 tctcgcccgt cagcgtttcg cccacggtgt acagacatta tttttcgacc atccgaactg
    26101 cattgccttt agccgcagtg gcaccgacga atatcccggc tgcgtggtgg tcatgtcgaa
    26161 cggagatgat ggcgaaaaaa ccattcatct gggagagaat tacggcaata aaacctggcg
    26221 tgattttctg gggaaccggc aagagagcgt agtgacagac gaaaacggcg aagcgacctt
    26281 cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt aaatccatcc
    26341 ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc gctttatcca
    26401 gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata ccatcgtact
    26461 caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta acgttagtga
    26521 gatcatcaga catcaatgtc aacttacccg gcacggcaat cccccgctgc cactgacggc
    26581 aatccaaggt atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc ggactgacca
    26641 gcgcacgttg cggccctttg gtctgccagt agcccgccag ccagtccggc gcgggggttt
    26701 tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg agcgcgcctg
    26761 caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt gtggcattaa
    26821 agctctgatg tcgccagcct ttccaggttc agagagaaat ttgatctaac tattattttt
    26881 gtgaattatt accccgaaaa taaatttgca tggtgtatga ttcgcggctc ttgtttcaaa
    26941 ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg cctggttgat
    27001 taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca cttactattt
    27061 tggcattact ggcacctttt gggctgtcac gggtgaattt acccgttggg gcggtcagct
    27121 cctgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta tccatctgga
    27181 aggatcgcca ttaacccgga tcgacggcat gatgatcctc ggtatgtttg gcggctgctt
    27241 tgctgctgcg ctgtgggcca acaatgtcaa actacgcatg ccacgcagcc gtatccgcat
    27301 tatgcaggcc atcattggcg gcattatcgc cggttttggc gcgcgtctgg caatgggctg
    27361 taacctggcg gcgttcttta ccggcattcc tcagttctcg ctgcatgcct ggttctttgc
    27421 catcgccact gccattggtt catggtttgg cgcgcgcttt accctgctgc ccatcttccg
    27481 tattcccgtg aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa aaccggatca
    27541 ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg gctgggcgct
    27601 gctcacggcg atgaaccaac caaaactggg gctggcaatg ctgtttggcg tcggctttgg
    27661 tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata tgtggatcac
    27721 cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtgagtg ccatcgggat
    27781 cttcagttac gtacagttag gcgttgaacc taaaatcatg tgggcgggac caaatgcggt
    27841 aattggtggt ttactttttg gttttggcat cgtgctggca ggcggctgcg aaaccggctg
    27901 gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg gcaacgtgat
    27961 cggctcaacg attctggcgt attactggga tgatttcgct ccggcgctgg ccaccgactg
    28021 ggacaaaatc aacctgctga aaacttttgg tccgatggga ggcctgctgg tgacatattt
    28081 gctgctgttt gctgcgctga tgttgattat cggctgggaa aaacgcttct tccgccgtga
    28141 ggcaccgcag actgctaagg agatcgcatg aaaaatatcg ttcctgatta ccgccttgat
    28201 atggtgggtg aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat gccgcaatta
    28261 aaaaaaggtg agatcctgga agtggtgagc gactgtccgc agtcgatcaa taatattcca
    28321 ctggatgcgc gtaatcacgg ctacacggtg ctggatattc agcaagacgg gccgaccatt
    28381 cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg gaacatcgtt
    28441 acctctgaca catagatttt acccgccccc tctcctattc ttttcagtat gatctcagga
    28501 ggttgctatg tgtggacgct ttgcccaatc ccaaacgcgt gaggattacc ttgcgcttct
    28561 cgcggaagat attgaacgcg atattcccta cgatcccgaa cccattggca gatacaatgt
    28621 cgcgccggga accaaagttc ttctgctcag tgaacgtgat gaacaccttc atctggatcc
    28681 ggttttctgg ggatatgctc ccggatggtg ggataaaccg ccgctgatta acgcccgcgt
    28741 agaaactgcc tcaaccagtc gcatgtttaa accgctctgg caacatggtc gggcaatctg
    28801 ttttgccgat ggctggtttg agtggaaaaa agaaggcgac aaaaaaagcc ttattttatc
    28861 tatcgcgctg acggacaacc tgtttttatg gccgcgatag gtagcatacc gtttgagcgt
    28921 ggtgacgaag ccgaaggatt tttgatcgtc actgcggcgg cagatcaagg tctggtagat
    28981 attcatgacc gccgcccgct ggttctgtcg ccagaagctg cacgggaatg gatgcggcaa
    29041 gacattggtg gtaaagaagc ctcagaaatc gctaccagaa gctgtgtgcc agcaaaccag
    29101 ttcacctggc accctgtgtc gcgcacggtg ggtaatgtca aaaaccaggg cgcggagtta
    29161 attcaacctg tttgttgaca tcctccacgc cctgaatgac gaggacccct gctacgttca
    29221 ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gagcggcctg actgcaccat
    29281 ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagctc catttacatc
    29341 ggcgttcgct gtatatccac atacctggca tctgaattta ctttgtgaca ggcg
//
LOCUS       DAJVQR010000045        29188 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVQR010000045 DAJVQR010000000
VERSION     DAJVQR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..213)
                     /locus_tag="OQM52_002123"
     CDS             complement(<1..213)
                     /locus_tag="OQM52_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCS1563971.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OQM52_002124"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OQM52_002124"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1563972.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OQM52_002125"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OQM52_002125"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1563973.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OQM52_002126"
     CDS             2894..3634
                     /locus_tag="OQM52_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1563974.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OQM52_002127"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OQM52_002127"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1563975.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OQM52_002128"
     CDS             complement(4192..4794)
                     /locus_tag="OQM52_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS1563976.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="OQM52_002129"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="OQM52_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1563977.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="OQM52_002130"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="OQM52_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1563978.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="OQM52_002131"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="OQM52_002131"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1563979.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OQM52_002132"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OQM52_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1563980.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OQM52_002133"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OQM52_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1563981.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="OQM52_002134"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="OQM52_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1563982.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="OQM52_002135"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="OQM52_002135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1563983.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="OQM52_002136"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="OQM52_002136"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1563984.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OQM52_002137"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OQM52_002137"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1563985.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OQM52_002138"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OQM52_002138"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1563986.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="OQM52_002139"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="OQM52_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1563987.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="OQM52_002140"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="OQM52_002140"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1563988.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="OQM52_002141"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="OQM52_002141"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1563989.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OQM52_002142"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OQM52_002142"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1563990.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="OQM52_002143"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="OQM52_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /locus_tag="OQM52_002144"
     CDS             20653..21009
                     /locus_tag="OQM52_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1563991.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="OQM52_002145"
     CDS             21336..21995
                     /locus_tag="OQM52_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1563992.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="OQM52_002146"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="OQM52_002146"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1563993.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OQM52_002147"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OQM52_002147"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1563994.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /locus_tag="OQM52_002148"
                     /pseudo
     CDS             complement(24948..25604)
                     /locus_tag="OQM52_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OQM52_002149"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OQM52_002149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1563995.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="OQM52_002150"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="OQM52_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1563996.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTNT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="OQM52_002151"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="OQM52_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="OQM52_002152"
     CDS             27338..27679
                     /locus_tag="OQM52_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1563997.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..29149)
                     /locus_tag="OQM52_002153"
                     /pseudo
     CDS             complement(28072..29149)
                     /locus_tag="OQM52_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
BASE COUNT         7374 a         7174 c         7505 g         7135 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aaccaacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac
    29041 ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc
    29101 gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc
    29161 tccattaacg gactgtctct cccttgtc
//
LOCUS       DAJVQR010000046        29035 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVQR010000046 DAJVQR010000000
VERSION     DAJVQR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            111..1142
                     /gene="mdtN"
                     /locus_tag="OQM52_002154"
     CDS             111..1142
                     /gene="mdtN"
                     /locus_tag="OQM52_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1563998.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1142..3184
                     /gene="mdtO"
                     /locus_tag="OQM52_002155"
     CDS             1142..3184
                     /gene="mdtO"
                     /locus_tag="OQM52_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1563999.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3181..4647
                     /gene="mdtP"
                     /locus_tag="OQM52_002156"
     CDS             3181..4647
                     /gene="mdtP"
                     /locus_tag="OQM52_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1564000.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4845..5084
                     /locus_tag="OQM52_002157"
     CDS             4845..5084
                     /locus_tag="OQM52_002157"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564001.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5094..5264
                     /locus_tag="OQM52_002158"
     CDS             5094..5264
                     /locus_tag="OQM52_002158"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1564002.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5313..6725
                     /gene="fdhF"
                     /locus_tag="OQM52_002159"
     CDS             5313..6725
                     /gene="fdhF"
                     /locus_tag="OQM52_002159"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1564003.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6726..6992
                     /locus_tag="OQM52_002160"
     CDS             6726..6992
                     /locus_tag="OQM52_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCS1564004.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7086..7775
                     /locus_tag="OQM52_002161"
     CDS             7086..7775
                     /locus_tag="OQM52_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1564005.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7853..9166)
                     /gene="gltP"
                     /locus_tag="OQM52_002162"
     CDS             complement(7853..9166)
                     /gene="gltP"
                     /locus_tag="OQM52_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1564006.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9508..10104)
                     /gene="nrfG"
                     /locus_tag="OQM52_002163"
     CDS             complement(9508..10104)
                     /gene="nrfG"
                     /locus_tag="OQM52_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1564007.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10101..10484)
                     /gene="nrfF"
                     /locus_tag="OQM52_002164"
     CDS             complement(10101..10484)
                     /gene="nrfF"
                     /locus_tag="OQM52_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1564008.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10477..12195)
                     /locus_tag="OQM52_002165"
     CDS             complement(10477..12195)
                     /locus_tag="OQM52_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1564009.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RAWDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12215..13171)
                     /gene="nrfD"
                     /locus_tag="OQM52_002166"
     CDS             complement(12215..13171)
                     /gene="nrfD"
                     /locus_tag="OQM52_002166"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1564010.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13168..13839)
                     /gene="nrfC"
                     /locus_tag="OQM52_002167"
     CDS             complement(13168..13839)
                     /gene="nrfC"
                     /locus_tag="OQM52_002167"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1564011.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13836..14402)
                     /gene="nrfB"
                     /locus_tag="OQM52_002168"
     CDS             complement(13836..14402)
                     /gene="nrfB"
                     /locus_tag="OQM52_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1564012.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14447..15883)
                     /gene="nrfA"
                     /locus_tag="OQM52_002169"
     CDS             complement(14447..15883)
                     /gene="nrfA"
                     /locus_tag="OQM52_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1564013.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16276..18234
                     /gene="acs"
                     /locus_tag="OQM52_002170"
                     /pseudo
     CDS             16276..18234
                     /gene="acs"
                     /locus_tag="OQM52_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18434..18748
                     /locus_tag="OQM52_002171"
     CDS             18434..18748
                     /locus_tag="OQM52_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1564014.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18745..20394
                     /gene="actP"
                     /locus_tag="OQM52_002172"
     CDS             18745..20394
                     /gene="actP"
                     /locus_tag="OQM52_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1564015.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20572..21864
                     /locus_tag="OQM52_002173"
     CDS             20572..21864
                     /locus_tag="OQM52_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1564016.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22018..23667)
                     /locus_tag="OQM52_002174"
     CDS             complement(22018..23667)
                     /locus_tag="OQM52_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1564017.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23818..25167)
                     /gene="ghxP"
                     /locus_tag="OQM52_002175"
                     /gene_synonym="yjcD"
     CDS             complement(23818..25167)
                     /gene="ghxP"
                     /locus_tag="OQM52_002175"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1564018.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25873..26337)
                     /gene="soxR"
                     /locus_tag="OQM52_002176"
     CDS             complement(25873..26337)
                     /gene="soxR"
                     /locus_tag="OQM52_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1564019.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26423..26746
                     /gene="soxS"
                     /locus_tag="OQM52_002177"
     CDS             26423..26746
                     /gene="soxS"
                     /locus_tag="OQM52_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1564020.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26749..28326)
                     /gene="pdeC"
                     /locus_tag="OQM52_002178"
                     /pseudo
     CDS             complement(26749..28326)
                     /gene="pdeC"
                     /locus_tag="OQM52_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28755..>29035
                     /locus_tag="OQM52_002179"
     CDS             28755..>29035
                     /locus_tag="OQM52_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCS1564021.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7281 a         7775 c         7350 g         6629 t
ORIGIN      
        1 agcgggcaaa tatcaatctg gcatttgtcg gaattattta taccgccctt tttgctctct
       61 acgccatgct gttctggctg gcatttttct aatctatttg agacgcgctc atggaaagta
      121 cgccgaaaaa agcacctcgc agtaaattcc ctgctctgtt agtggttgcg ttggcgctgg
      181 ttgcccttgt tttcgttatc tggcgcgtag acagtgcgcc atcaactaat gacgcttacg
      241 cgtcagcaga taccattgat gtggtgccgg aagtcagcgg tcgcattgta gaactggcgg
      301 tcaccgacaa ccaggcggtg aaacagggcg atttgctgtt ccgcatcgac ccgcgcccgt
      361 acgaagccaa cctggcgaaa gccgaagcct ccctcgcggc gctggataag caaattatgc
      421 tcacccagcg tagcgttgac gcccaacagt ttggtgccga ctcggttaat gccacggtag
      481 aaaaagcccg tgccgccgcg aaacaggcca cagatacatt acgccgcacc gagccattac
      541 tgaaagaggg ttttgtctca gcagaagacg ttgaccgtgc aagaacggcg cagcgcgccg
      601 cagaagcgga tcttaatgcc gtattgttac aggcgcagtc agccgccagc gccgtcagcg
      661 gcgtcgatgc gctggtggcg caacgtgcgg cggtcgaagc ggatattgcc ctgaccaaac
      721 tacatctgga aatgacgacc gttcgcgcgc catttgatgg ccggatcatt tccctcaaaa
      781 cctccgtcgg gcaatttgct tctgccatgc gccctatttt taccctcatc gacactcgtc
      841 actggtacgt gatcgccaac ttccgcgaaa ccgatctgaa aaatattcgc tcaggtacac
      901 ccgcaacgat tcgcctgatg agtgacagcg gcaaaacctt cgagggtaaa gtggattcga
      961 ttggctacgg cgtgctaccg gatgacggcg gcctggtgct gggcggcctg ccaaaagtgt
     1021 ctcgttctat taactgggtc cgcgttgccc agcgttttcc ggtcaaaatc atggtcgata
     1081 aacctgaccc ggaaatgttc cgcatcggcg cttcggcagt cgctaatctt gagccgcaat
     1141 aatgagcgcg ctcaactccc tgccattacc ggtggtcagg ctgctggcgt tctttcatga
     1201 agagttaagc gagcggcgac ccggccgcgt gccgcagatc gtgcaactct gggtaggctg
     1261 cctgctggtg attctgatct cgatgacttt tgagatccct tttgtggcgt tatcgctggc
     1321 agtgctgttt tacggtattc agtcgaacgc gttttacacc aaatttgtgg cgatcttgtt
     1381 tgtggttgcc acggtgctgg agatcggcag cctgtttttg atctacaaat ggtcatacgg
     1441 cgaaccgttg atccgattga tcatcgccgg gccgatcctg atgggctgca tgtttttgat
     1501 gcgcacccat cggctgggac tggtcttttt cgctgtcgcc atttacgggc aaaccttccc
     1561 cgccatgctc gactatccgg aagtagtcgt gcgcttaacg ctgtggtgta tcgttgttgg
     1621 cctctatcca accttgctga tgacgttaat cggcgtgctg tggtttccca atcgtgccat
     1681 tacgcaaatg catcaagcgc ttaatgatcg gcttgatgat gccattagcc acctgacgga
     1741 cagcctcgca ccgctacccg aaacgcggat tgaaagagag gcgctggcgc tgcaaaaact
     1801 gaatgtcttt tgtctcgcgg acgatgccaa ctggcgaact caaagcgcat ggtggcaaag
     1861 ctgcgtggca acggtaacct acatttactc gacgctgaat cgctacgatc ccacctcttt
     1921 tgctgattct caggcaatta ttgaatttcg gcaaaaatta gcctcagaaa tcaacaagct
     1981 gcagcatgcc gttgctgaag gtcagtgctg gcaaagcgac tggcggctca gtgaaagtga
     2041 agcggtggcg gcacgggaat gtaacctgga gaatatctgc cagacgttgt tacaactggg
     2101 ccagatgaac ccgaatacgc cgccaacgcc cgccgccaaa ccgccatcaa tggtcgccga
     2161 tgcttttacc aatccagact atatacgcta cgcggtaaaa acattgctcg cctgtttgat
     2221 ctgttacacc ttttacagcg gcgtggactg ggaaggcatt cacacctgta tgctgacctg
     2281 cgtgatcgtc gctaatccaa atgtcggttc gtcgtaccag aagatggtgc tgcgttttgg
     2341 cggggccttt tgcggcgcga ttctggcgct gttattcacg ctactggtca tgccctggct
     2401 ggacaatatt gtcgaattgc tgtttgtgct ggcaccgatt ttcctgttgg gcgcatggat
     2461 tgccaccagc tctgaacgct cttcttatat cggcacacag atggtggtca ccttcgcgct
     2521 cgccacgctc gaaaacgttt ttggcccagt gtacgacctg gtggaaattc gcgatcgcgc
     2581 cctgggtatc atcattggta ccgtggtgtc cgcgatgatt tacacctttg tctggcctga
     2641 aagtgaagcg cgcacgctgc cgcaaaaact ggctggcgcg ctgggtatgt taagtaaagt
     2701 aatgcggatc ccacgccagc aggaagtcac ggctctgcgc acttatctgc aaattcgtat
     2761 cggtctgcat gcggcgttta atgcctgtga agagatgtgc caacgcgtgg cgctggagcg
     2821 tcaactggac agcgaagaac gcgccttact gattgaacgt tcgcaaacgg ttattcgtca
     2881 gggccgcgat atccttcacg cctgggatgc cacctggaat tcggcgcagg cgctggataa
     2941 cgcactacag ccggacagag caggtcagtt tgccgacgcc ctggagaaat acgctgccgg
     3001 tctggcaacc gcactcagcc gttctcctca aataacgctt gaagagacac ccacctcgca
     3061 ggccatcctg cccaccttat taaaacagga gcaacacgtc tgccagcttt tcgcccgctt
     3121 gccagactgg acagccccgg cattaacgcc cgccacggaa caggcacaag gagccacgca
     3181 atgatcaatc gtcaactttc acgtctgctg ttgtgcagca ttctcggcag cacgacgctg
     3241 atttccggct gtgccctggt acgtaaggat tctgcgcctc atcaacagct caaaccggaa
     3301 caaatcaaac tggccgatga tattcatctt gccagctccg gctggccgca ggcgcagtgg
     3361 tggaaacaac tcaatgaccc gcagttggat gccctgatcc aacggacgct aagtggttca
     3421 cacaccctcg ccgaagcgaa actgcgggaa gaaaaagcgc aatcgcaggc cgatttgtta
     3481 gatgccggtt cacagttgca ggtggcagcg ttagggatgc ttaaccgcca acgtgtctcg
     3541 gcgaacggct ttttaagccc ttatgcgatg gatgcgcccg cactggggat ggacgggccg
     3601 tactatacgg aagccacagt aggtttgttt gccggactgg atcttgattt gtggggtgtg
     3661 catcgctcag cggttgccgc cgccattggc gcgcataatg ccgcgctggc agaaaccgca
     3721 gcagtagagc tatcgctgac cacgggcgta gcgcagcttt attacagtat gcaggccagc
     3781 tatcagatgc tcgatctgtt agaacaaact cgcgatgtga ttgattacgc ggtgaaagcg
     3841 caccaaagta aagtggcgca cggtctggaa gcacaagtgc ctttccacgg cgcgcgggca
     3901 cagattctgg cggtcgataa acaaattgct gccgtcaaag ggcaaatcac cgaaacgaga
     3961 gaatctctgc gcgcattgat tggcgcggga gccagcgata tgccggagat caaacctgtg
     4021 gcattaccac gagtccagac cggcattccg gcgacactct cttatgagtt gctcgccaga
     4081 cgcccggatc tgcaagccat gcgctggtat gttcaggcgt cattagatca ggtggattcc
     4141 gcgcgggcgt tgttctatcc gagctttgat atcaaagcgt ttttcggcct tgatgccatc
     4201 cacctggata ccttattcaa aaaaaccagt cgccagttca acttcatccc gggtctgaaa
     4261 ttgccgctgt ttgacggtgg acggttgaat gccaatctcg aaggcacgcg cgccgccagc
     4321 aacatgatga ttgaacgtta caaccagtca gtactgaacg cggtgcgtga cgttgccgtc
     4381 aacggcacgc gtctgcaaac gctcaacgac gagcgagaaa tgcaggctga acacgtggaa
     4441 gcaacgcgct tcacccagcg cgctgccgag gccgcctatc agcgcggctt aaccagccgc
     4501 ttacaggcca ctgaagcccg gttgccagta cttgccgaag agatgtcatt actgatgctg
     4561 gacagccgcc gggtgatcca aagcattcag ttgatgaaat cgctgggcgg cgggtatcag
     4621 gcggctcccg tcgtcgagaa aaaataaaat gtctgccgcg tgatggctgt cacgcggtat
     4681 ttcgtttcgt cacgtcaaaa ctgacgacag cctgtttttc gtcagagttt tgaataaata
     4741 gtgcccgtaa tatcagggaa tgaccccaaa taaaatgtgg cataaaagat gcatactgta
     4801 gtcgagagcg cgtatgcgtg atttgattaa ctggagcgag accgatgaaa aaagtcgtca
     4861 cggtttgccc ctattgcgca tcaggttgca aaatcaacct ggtcgtcgat aacggcaaaa
     4921 tcgtccgggc ggaggcagcg caggggaaaa ccaaccaggg taccctgtgt ctgaagggtt
     4981 attatggctg ggatttcatt aacgataccc agatcctgac cccgcgcctg aaaaccccca
     5041 tgatccgtcg ccagcgtggc ggcaaactcg aacctgtttc ctgagatgag gcactgaatt
     5101 acgttgccga gcgcctgagc gccatcaaag agaagtacgg tccggatgcc atccagacga
     5161 ccggctcctc gcgtggtacg ggtaacgaaa ccaactatgt aatgcaaaaa tttgcgcgcg
     5221 ccgttattgg taccaataac gttgactgct gcgctcgtgt ctgacacggc ccatcggttg
     5281 caggtctgca ccaatcggtc ggtaatggcg caatgagcaa tgctattaac gaaattgata
     5341 ataccgattt agtgttcgtt ttcgggtaca acccggcgga ttcccaccca atcgtggcga
     5401 atcacgtaat taacgctaaa cgtaacgggg cgaaaattat cgtctgcgat ccgcgcaaaa
     5461 ttgaaaccgc gcgcattgct gacatgcaca ttgcactgaa aaacggctcg aacatcgcgc
     5521 tgttgaatgc gatgggccat gtcattattg aagaaaatct gtacgacaaa gcgttcgtcg
     5581 cttcccgtac agaaggcttt gaagagtatc gtaaaatcgt tgaaggctac acgccggagt
     5641 cggttgaaga tatcaccggt gtcagcgcca gtgagattcg tcaggcggca cggatgtatg
     5701 cccaggcgaa aagcgccgcc atcctgtggg gcatgggtgt aacccagttc taccagggcg
     5761 tggaaaccgt gcgttctctg accagcctcg cgatgctgac cagtaacctc ggtaagccgc
     5821 atgcgggtgt aaacccggtt cgtggtcaga acaacgtgca gggtgcctgc gatatgggcg
     5881 cgctgccgga tacgtatccg ggataccagt acgtgaaaga tccggctaac cgcgaaaaat
     5941 tcgccaaagc ctggggcgtg gaaagcctgc ctgctcatac cggctatcgc atcagcgagc
     6001 tgccgcaccg cgcagcgcat ggcgaagtgc gtgccgcgta cattatgggc gaagatccgc
     6061 tgcaaactga tgcggagctg tcggcagtac gtaaagcctt tgaagatctg gaactggtca
     6121 tcgttcagga catctttata accaaaaccg cgtcggcggc ggatgtcatt ttgccgtcaa
     6181 cgtcgtgggg cgagcatgaa ggcgtgttta ctgcggctga ccgtggcttc cagcgtttct
     6241 tcaaagcagt tgaaccgaaa tgggatctga aaacggactg gcaaatcatc agtgaaatcg
     6301 ccacccgtat gggttatccg atgcactaca acaacaccca ggagatctgg gatgagttgc
     6361 gtcatctgtg cccggatttc tacggtgcga cttacgagaa aatgggcgaa ctgggcttca
     6421 ttcagtggcc ttgccgcgat acttcagatg ccgatcaggg gacttcttat ctgtttaaag
     6481 agaagtttga taccccgaac ggtctggcgc agttcttcac ctgcgactgg gtagcgccaa
     6541 tcgacaaact caccgacgag tacccgatgg tactgtcaac ggtgcgtgaa gttggtcact
     6601 actcttgccg ttcgatgacc ggtaactgtg cggcactggc ggcgctggct gatgaacctg
     6661 gctacgcaca aatcaatacc gaagacgcca aacgtctggg tattgaagat gaggcattgg
     6721 tttgagtgca ctcgcgtaaa ggcaaaatta tcacccgtgc gcaggtcagc gatcgtccga
     6781 acaaaggggc gatttacatg acctaccagt ggtggattgg tgcctgtaac gagctggtta
     6841 ccgaaagctt aagcccgatt acgaaaacgc cggaatacaa atactgcgcc gtgcgcgtcg
     6901 agccgatctc cgatcagcgc gccgccgagc agtacgtgat tgacgagtac aacaagttga
     6961 aaactcgcct gcgcgaagcg gcactggcgt aatacagtcc tttctacagc ctcctttcgg
     7021 aggctgtttt tttatccatt cgaactcttt atactggtta ctccctaccc aatcgtatta
     7081 tcaaaatgaa aaaaattatc gcattgatgt tgtttttgac attctttgcc cacgcgaacg
     7141 actccgagcc tggcagccag tatttaaagg cagcagaggc cggggaccga cgtgcacaat
     7201 attttcttgc cgacagctgg tttagctccg gcgatttgag caaagccgaa tattgggcac
     7261 agaaagccgc cgacagcggt gatgctgatg cctgcgcgct gttggcgcag atcaaaatca
     7321 ccaatccggt cagtctggac tatccacaag caaaagttct tgcagagaaa gcggcgcaag
     7381 cgggcagtaa agaaggtgaa gtaacgctgg cgcatattct ggtaaatact caagcgggta
     7441 aaccggatta tccaaaggcg atttcgctgt tagaaaacgc ctcggaagat ctggagaatg
     7501 actccgccgt cgatgcccaa atgctgcttg gtttgattta cgccaacggc gtgggcatta
     7561 aggccgacga tgacaaggca acctggtatt tcaaacgcag ctctgcaatt tcccgaaccg
     7621 gttattccga gtactgggcg ggaatgatgt tcttaaacgg tgaggaaggt tttatcgaga
     7681 agaacaaaca aaaggcgctg cactggttga acctgagctg tatggaaggg tttgataccg
     7741 ggtgtgaaga gtttgaaaaa ttaaccaacg gttaaggtag gcctgataag acgcggcaag
     7801 cgtcgcatca ggcatccaca cattgccggg ggatatcccc ggcaatcttc aattattgat
     7861 ccgcagtttt atcaaatttg cccagcactt cacgctcata agccagcgcc tttttgcggt
     7921 caaatttgtg ttcccacttg gcaatcacca gcaccgccag cgcattaccc accacgttca
     7981 gcgcagtacg cgccatgtcg aggatacggt caacaccagc aataaacgcc agaccttcca
     8041 gcggaatacc tacgctaccc agcgttgcca gcaacaccac aaacgacacg ccagccacgc
     8101 cagcaatccc tttcgaggtc accatcagcg tcagcaccag aatgatttcc tgccagatgg
     8161 acaggtcaat gccatacagc tgagcgatga aaatagcggc aatactttga tacagcgtcg
     8221 aaccatcaag gttaaaagag taaccggtcg gcaccacgaa gctggtgatc gacgccggtg
     8281 ctccgtaggc ttccatcttc tcaataattc gcggcagtac gctttcagag ctggcagtgg
     8341 agtacgccag aatcagctca tctttcagaa tacgaatcag gatccagacg cttaacccgc
     8401 acagacgtgc cacaattccc agcactacca gcgcgaagaa cagaatggcg aaatgcacca
     8461 gcagcaccag tttcgccagt ggccacagag acgagaaacc aaagttagcc accgtcaccg
     8521 caatcagcgc aaacacaccc accggtgcat aacgcatcac catgtgagtc actttaaaca
     8581 tggtttcaga gatggagcgg aacacggtca ccagcggttc acgatgcgtc gcgggcaggg
     8641 aagaaagccc cagaccaaac agcaccgaga aaaagatgat cggcagcatt tcgcctttcg
     8701 ccatcgacgc cacaatgttc gtcggcacca gcgacaaaat cgtgcccata atgccgtggg
     8761 aactgctttg taccgcttcc gtagtgctct gatatttcga gatatcgacg gtcgccagct
     8821 gcgacatatc caccccggca ccgggctgga agacgttcgc cagagtgatc cccaaaatga
     8881 tggcgacggt ggtgatcacc tcgaagtaaa taatggtttt cgcgccaata cgcccgagct
     8941 gtttggcatc accaacaccc gcgataccca ccaccagcgt ggagatcaca atcggcacaa
     9001 caatcatttt gatcagatgg atgaagatat cacccgccgg agagagcaaa ttgacgacca
     9061 gccagtcgcg gctgtcgcta tggtagtgca ggtagcttcc caggagaatg cccagcacca
     9121 tagcaaacag aatctgccag gccaggctga attttatatt tttcatcaga ataacttcct
     9181 caatggagcg ccgttccccg ttcagaaacg cgagaacgtg acatactgcc agggcatgaa
     9241 ttgtgttgcg ttggtttgta gattttttta acgcttatga tcagtatcat ttgcgcggta
     9301 tgttgcgcaa ccctttaaca acagagcttt tcgctgcatt aatgcataat aacgcgagtt
     9361 tatggcagga ttaatgaata accatttgtt atggaaagat ttttatttgt ttaatctgca
     9421 ataatactga ttggtgatta tttttggcat atgttcattc gattattttt tatgcagctc
     9481 aatgacataa tcttcatcac cagatgatta atccgatctc cgctgcaaca gttttgccat
     9541 attaatcgac tcgaccagct gtgtccggtt gatccggggt gagttgagat ccatcacttt
     9601 ttgccatagt tcgatggctt gcgcgtaatt cgcctgcata aacgcatcgg aagccagcag
     9661 catcagagcg gtgatttcgt tagagtccag cgcgagggct ttgtcgatca ttgcgcgagt
     9721 ctgggcggtc atatgctggc tggcctggta ataaagcacc gtcgccagcg ccgcatacag
     9781 ttcagcgttc tcaccacgta gttgcaacgc ctgacgatac gccagcagcg aattgctgta
     9841 atcgttttgc cacagatagt actcgcccag taacgcccat tgttcgctgt tttgtggatt
     9901 agcgcggatt ttatcctgca atgcctgaag ctgcgcttcc ggggtttgct ggctggcaaa
     9961 ctgatgtagc ggatcgcgct gacgctgata ctccgcacgt accgcctgcc attttgggct
    10021 taacagataa ctgccgacac agaggaatac catcaatatc gtgagtgttg tcagcatttt
    10081 caccggtatt tgaggctgtt tcatcgctta gccctcactc gccagaggat cagtgccatc
    10141 agcaataaca acaccactgg cagcgcccac aacaccagcg tctgacccgt taacggtggg
    10201 ttatagcgaa caaaatctcc gtagcgttcg gtcatccagc caatgatttc gacttcgttc
    10261 ttcccctccg acaccatgct ataaacctga tggcgcatac tgaccgccac cggcgcattg
    10321 gattcgagta agttttgatt ctggcactgc ggacaacgta actggctggc aatatttaac
    10381 gcctgttgct gttgttgcgg attggcgaat tgccaggtgt ctacaacctg agcgcgcgca
    10441 aaacaggtaa ataacagcag tagcgtgaga agccctttat tcatcgcgct tcttcccccg
    10501 ccatccgctt aacaatgcgc ccgcaatcat caacaatcct cccccccaga tccagcgcac
    10561 accgctttgt acatacaaac gaaaagcgta acgatccgct ctggtttttt cacccatgac
    10621 cgcataccag tcatggatgc cgttccagcg aattgacggt tccatcattt gctgacggcg
    10681 tgcttcataa aaacgccgct ccggcgttaa ttcaccaatg cgttgctgat ggtcaaacag
    10741 cgccactatc gctttttcgc tggtgtaatt gcctctggct tgcagatcga gtcgctcaaa
    10801 acggaaggtg tatcctgcca gcgtcacctg ctgacccggc tgtaaattca ggctgatctc
    10861 ctgacgactg acactggaaa ccacgatccc cgcggcaaat aacagcacgc cagcatgagc
    10921 taccagcgcc ggaagctgca cgcgtttgcc agagacaaac gtcgccagca caatcaccac
    10981 cagcatcaac agaccaaacg gtaacgtcgc gcggttaaaa tacggcgcgc caacggagag
    11041 gcgtccccag cccagcaggc cgtaaatcat cggataaagc gttcccacca gcacgatcag
    11101 tagcactgcg caaaacagca acagcgtagc gaggattaac atttcccgcg ataaccccga
    11161 aaaacgcacc gccgggccac cgtcccacgc tcgccagcca tacagagcca aagacgcaag
    11221 gctaatcagt gcaaacaggc tgaacaacgg cacggcgcgg acgttatcca gtgcgaacgc
    11281 atgaaccgaa accagaatgc cagaacggac aattaaggtg cccagcagcg acagcatcag
    11341 agtaactatc gccagcaaca gcgaccagtg gcggaaaatc ccccgctggc gtgtcaggga
    11401 caaactgtgc agcagcgcag tggcagaaag ccagggtaat aaagaggcgt tttccaccgg
    11461 atcccagaac caccagccgc cccagccgag ttcgcagtag gcccaccagg aaccgaggat
    11521 gatccccgcc gttaatgcac tccagccagg taacgcccag cgccagcaaa ttcgggcgct
    11581 ggccgcatca aactcgccgc gcagtagact cgccagcgcc acgctcgccg ctaccatcaa
    11641 accgccatag ccaaggtaaa gcagcggtgg atgaaagata agaccgggat gttgcagcat
    11701 cggattgaga tcgcggcctt cgattgctgg tggaaatatc cgcacgaagg gatcggacca
    11761 cagcaccaca aacagtagca gtgcggcgag catgagagat aaaacggcta gcgtcagcgg
    11821 aaatagcgga tcgctttgct gccgataatg ccaggcaaac agcgcgctcc aggcggaaag
    11881 cagcagcacc caaagcagca gcgaaccttc atgaccgccc cacaccgccg ccagtttgag
    11941 ttcccacgac agcaggctgt agctatgttg ggcgacataa atgaccgaga aatcgctgat
    12001 gagaaaacaa tacgtcagta cgccaaaggc gagcagcagg agcgcgaact gcgccagaat
    12061 gccgatgcaa gtgagtcgca tcatggcagg ccagcgcaac cgcactcccg caaaggccgt
    12121 caacggggtc aacacgttga ccccgagact taacaacaac gccagaaaac cgacttcagg
    12181 aaggaatacg tccagaaacc acctcttttc tggcttacgc caccgtcaac tgtccggcgt
    12241 agagaatgaa aaagcgcagc atcaacacgc cgaccagact ggccccacac gccgccagca
    12301 cggcaggaat gccggaactg cgattgaccc acggtttgag caacattggc acaatcagcc
    12361 ccagcccggc gacaccaagc cagaaccacc acgtccagaa accgccacct aatgccgcca
    12421 ccagcgcacg aactttaccg tcatcgccca gcgccagacc gacaaaaaac gccaccagca
    12481 ggaagatttc accccatacc acggggattt ccatccggtg aacaaagtgc gcttccgtgg
    12541 aatgcgggtt actgcgttgg cgtatcgcca tggcgatcag cgccaccgcc gcaccggacg
    12601 agatgccgga gaagaggagc agcaccggca ggctcgggtt attgaggaac ggatacgatt
    12661 tcagcgccga cagcagaaag ccggtataag cccccagcag caccgccaac accagcatca
    12721 gcgtttccag tccgcgatga acgggcgtca gcaggctcag aaccttttgc acgatcccca
    12781 gcttcggcaa ccagcgttgt tgcagggcaa gcaaatcatg ttcaaagatt ttcgccagcc
    12841 acagcaccag caccaccatg tagagctgaa acagcatcac ccccatcgac atcaccgagg
    12901 taaaactgta gtggaacatc agcttccaga aggtccacgg tcttgtcagg tggaagacga
    12961 ggatcaacag accgaggatc accgcgcccg gcccgacaat cagcgtggtg cgcagcaacg
    13021 tactgtctgc accgcccgcc tgcgggtaga agcgacgtaa cagcacggcc agcgtcacca
    13081 gaccggcaga aataccaatc aaaaacaggt agatggcaat cggccagtcc cacaccagcg
    13141 attcaaaatg aaatgcggaa gtctgcgtca ttggctcacc tccccgtatt taaacggtac
    13201 gcggtacagc ttcggtttgg ttcccagcgc cagcttgtag cggtaagtag gcttctggcg
    13261 cagcagttgc gaaatctcac tgttaggatc gtccagattg ccaaacgtca gcgccttcgt
    13321 cgggcaagct tcaacgcacg cgggaagctt accggcctgc aaattggtct tacgacagaa
    13381 atcgcatttg tccgccgtct tcgtgaccgg atggataaag cgcacgcggt acggacaggc
    13441 ggcgatgcag tactgacaac cgacgcagag atcaggatta acatcgacaa tcccgctagc
    13501 ggcatcacga aaagacgcac cggtcgggca gacgtcaacg cacggcgcat gatcgcagtg
    13561 ctggcaagac ttacggaaga agcgatattt cacatcagga aattcgccct gcggctcgct
    13621 acgaataatc gtcaggcgcg agacgccttc cggcaccttg ttcacttccc gacaagcatc
    13681 catacaggcg gtacagccga tgcataacga ctcgtcatgc accatgccgt aacgcacccc
    13741 attgatattc aacgttttcg ccaccacgcg ccctgcggtg ccgctgacgg ctgccagcac
    13801 gccgacgccg gtgagaaact ggcgacgaga ccaggtcatg gctgctcctt aagcaacgga
    13861 acagacgccg ggttaaagtt cggattggtg cgctgatcgc tgtggcaatc gacgcaaatc
    13921 ttaatccgtc ctttgtcact taacgtctgc atcgtatctt gctgtggatg cagggaatgg
    13981 cagctggcgc acgccacttt ggttacgtgg acatcgtgcg gccagaacgc tttttgcaac
    14041 tgttccggca gatgacagga catacagacg ctgttctgct ctccgacctt atacatcggc
    14101 tcgttaaagc gcatcacatc tttcacccct tcgcggtgtt gcggtgatgc ttggccgtga
    14161 cagttggtgc aggtgaccgg caatttgtta ttcgggttga taacggaagc atgtttgcca
    14221 tgcatacctt cggtatctgg tttatgacag tccagacagg cggcatccgg gttacgctgc
    14281 tgggtaactt cgtagcgatc accagatgcc tgcaccgcag gggtagcggt aattccgttc
    14341 aggctccaca acaggcccga cgccagcacc ccagcagtta ataacgaacg taatacgctc
    14401 atattcactc cattgtgttt tccgcccctt gcgaggcgga acggggttat tggcttaaca
    14461 gaccgttttt acgtgcctgc tcttcccact gcgggatcac cgttttgatg aagtcctgct
    14521 tctcggcctt gatttgttcc atgttcaggc caatggcctg ctgggctttc tctttggtcg
    14581 agatatccgg gatctggatt tcatgggtga tacctttggt cgccagcagg cgcgccagtt
    14641 tggtgcgcgc atccgccgct ttatccatcg ccgtaccgag catccgtaga ccttcttccg
    14701 gtgcgtgcat atgaataccg tgggaagcaa tcgccagatc ccaacgccac tgggcatgac
    14761 ggatatcgtc ctgaatcggc ttcatttcgg cttccgtcgc gcctgcatcc agcgccgctt
    14821 tcgcttcgaa gtgagcgtga accagttgat cttcaacctt gattttcagg tcgttaatcg
    14881 attgcttacg ttccgcgacc actttttgca aggcagcttt atcctgggta tggcagttcg
    14941 cacaagtctg ggcgaagtta tcaaacggat taccaatttt atggtcggtg tagagtttgc
    15001 cttcggcgtt ctgcactttt ggcatatggc agtcgataca ggtcacgttg tttttaccgt
    15061 gaatgcccgc tgtccaggtt tcatattccg ggtgctgcgc tttcagcatt ggcgttttcg
    15121 acagggagtt agtccagtca gagaaggcaa ttttgtcgta atactgctcc atattttcga
    15181 ctttcatgcc gtcatcccac gggaatttaa ccgctttgtt tttgccgtcg aagtaatact
    15241 ccacatggca ctgaccgcaa accatcgatt gctggtcgaa acgtccggct ttctcaaaag
    15301 gtttaccaat ggcttccatc gcgcgagccg catacggacg ggaaagggtt aactccggtt
    15361 tgcctttggc gaactctggt gaggcggtgt tatggcagtc ggcacaacct aagttgttga
    15421 cgatttccgg gccgccgcgc gcccatttac cgtggaagta gccatcttcg ccgtctttct
    15481 ggatcagacg cgccacatcc gggcttttac aactccagca cgccatcggt agcggaccat
    15541 cttcagcgtt tttcggcgca ccggtacgca gggtttcacg cacatcggtc acagcaaagg
    15601 catgtccacg aggcttgttg taatcgcgcg agaagggata ccccgcccac aggatcacca
    15661 gccgtggatc ttccgccagg gcgtcaacac gctctgactg ttccgaggtg gctttccagg
    15721 agagatattg atcgtgatgc tgcggggcaa aggtttcatt cttcgcttcc acagttacag
    15781 gttttgcggg agctgccgtt tgttcagcgt gaacagaagt gaaaaagaaa aaaggaatca
    15841 ataagctgaa gatacggcgt gcgtttattt ttatccttgt cataggggct tcatccgaat
    15901 tgcgccattg ttgcaatggc ggtttttatt gtttttcacg acagtaaccg cacctacact
    15961 gtcatgacat tgctcacccc tatgtgtaac aaataaccac actgtgaatg ttgtctttaa
    16021 tcaattgtaa gtgcatgtga aataccactt tagagttagt cagtatcttc ctctttttca
    16081 acagcatgca taactgcatg ttcctcaaag aattaatcaa cttttgttgg tgaccttcaa
    16141 aaattaccct gccgtttatt tgcaccattc tacttttgcg tgatctgtcg cccaaatact
    16201 aaacaaaact gccaataccc ctacatttaa cgcttatgcc acatattatt aacatcctac
    16261 aaggagaaca aaagcatgag ccaaattcac aaacacacca ttcctgccaa catcgcagac
    16321 cgttgcctga taaaccctca gcagtacgag gcgatgtatc aacaatctat taacgtacct
    16381 gataccttct ggggcgaaca gggaaaaatt ctcgactgga tcaaaccgta ccagaaggtg
    16441 aaaaatacct cctttgcccc cggtaatgtg tccattaaat ggtacgagga cggcacgctg
    16501 aatctggcgg caaactgcct tgaccgccat ctgcaagaaa acggcgatcg caccgccatc
    16561 atctgggaag gcgacgacgc cagccagagc aaacatatca gctataaaga gctgcaccgc
    16621 gacgtctgcc gcttcgccaa taccctgctc gagctgggca ttaaaaaagg tgatgtggtg
    16681 gcgatttata tgccgatggt gccggaagcc gcggttgcga tgctggcctg cgcccgcatt
    16741 ggcgcgatgc attcggtgat tttcggcggc ttctcgccgg aagccgttgc cgggcgcatt
    16801 attgattcca actcacgact ggtgatcact tccgacgaag gcgtgcgcgc cgggcgtagt
    16861 attccgctga agaaaaacgt tgatgacgca ctaaaaaacc cgaacgtcac cagcgtagag
    16921 catgtggtgg tactgaagcg tactggcgga aacatcgact ggcaggaagg gcgcgacctg
    16981 tggtggcacg acctggttga gcaagccagc gatcagcact aggcggaaga gatgaacgcc
    17041 gaagatccgc tgtttattct ctatacctcc ggttctaccg gaaaaccaaa aggcgtactg
    17101 cacactaccg gcggttatct ggtgtacgcg gcgctgacct ttaaatatgt ctttgattat
    17161 catccgggtg atatctactg gtgcaccgcc gatgtgggct gggttaccgg acacagttat
    17221 ttgctgtacg gcccgctggc ctgcggcgca accacgctga tgtttgaagg cgtaccgaac
    17281 tggccgacgc ctgcccgtat ggcgcaggtg gtggacaagc atcaggtcaa tattctctat
    17341 accgcaccca cggcgatccg cgcgctgatg gcggaaggcg ataaagcgat tgaaggcacc
    17401 gaccgttcgt cgctacgcat tctcggttcc gtgggcgagc caattaaccc ggaagcgtgg
    17461 gagtggtact ggaaaaaaat cggcaacgag aaatgtccgg tggtcgatac ctggtggcag
    17521 accgaaaccg gcggtttcat gatcaccccg ctgcctggcg ctaccgagct gaaagccggt
    17581 tcggcaacac gtccgttctt cggcgtgcaa ccggcgctgg tcgataacga aggtaacccg
    17641 ctggaagggg ctaccgaagg tagcctggtg atcaccgact cctggcctgg tcaggcgcgt
    17701 acgctgtttg gcgatcacga acgttttgag cagacctatt tttccacctt caaaaatatg
    17761 tatttcagcg gcgacggcgc gcgtcgcgat gaagatggct attactggat caccgggcgt
    17821 gtggacgacg tgctgaacgt ctccggtcac cgtctgggga cggcagagat agagtcggcg
    17881 ctggtggcgc atccgaagat tgccgaagcc gccgtagtag gtattccgca caatattaaa
    17941 ggtcaggcga tctacgccta tgtcacgctt aatcacgggg aggaaccgtc accagaactg
    18001 tacgcagaag tccgcaactg ggtgcgtaaa gagattggcc cgctggcgac gccagacgtg
    18061 ctgcactgga ccgactccct gcctaaaacc cgctccggca aaattatgcg ccgtattctg
    18121 cgcaaaattg cggcgggcga taccagcaac ctgggcgata cctcgacgct tgccgatcct
    18181 ggcgtggtcg agaagctgct tgaagagaag caggctatcg cgatgccatc gtaacccaca
    18241 attgccggat gcgaatcggt aacggtttgt aggcctgata agacgcgaca gcgtcgcatc
    18301 aggcattgat tgccggatgc ggcgtaaacg ccttatccgg cctacattcg gcaagggtta
    18361 cccgagcgtt aaccttctcc cataagggag cgggaattaa aacaatccct acattacctc
    18421 tggagaatct gtgatgaatg gtacgattta tcagcggata gaagacaatg cgcatttcag
    18481 ggagttagtc gaaaaacggc aacggtttgc caccatcctg tcgattatta tgctggcagt
    18541 ttatatcggc tttattttac tgatcgcctt cgcgcccggc tggctgggca cgccgctgaa
    18601 tccgaacacc agcgtcacac gcggtattcc aattggtgtt ggagtgattg tgatctcctt
    18661 tgttctcacc ggtatctaca tctggcgggc gaacggcgaa ttcgaccgtc ttaataatga
    18721 agtcctgcat gaggtacaag catcatgaaa agagttctga cggcgcttgc cgccacactc
    18781 cctttcgcag ctaacgccgc ggatgctatt agcggggccg tagagcgcca gccaacgaac
    18841 tggcaggcga ttattatgtt cctgattttc gtcgtgttta cgctcggcat tacctactgg
    18901 gcatcaaaac gcgtacgttc tcgtagcgac tactacaccg caggcggcaa tatcactggc
    18961 ttccagaacg ggctggcgat tgccggggac tatatgtccg ccgcctcatt cttggggatc
    19021 tccgcgctgg tgtttacctc cggctatgac gggctgatct actcgttggg cttcctggtg
    19081 ggctggccaa tcattctgtt tctgattgcc gaacgtctgc gtaacctggg acgctacacc
    19141 tctgccgatg tggcctctta tcgtctgaaa caagggccga ttcgtattct ttcggcctgt
    19201 ggttctctgg tagtggtggc gctttacctt atcgctcaga tggtaggcgc aggtaaactg
    19261 atcgagctgc tgtttggcct taactatcac attgcggtgg tgctggtcgg cgtgctgatg
    19321 atgatgtacg tcctgttcgg cggcatgctg gcgaccacct gggtacaaat tatcaaagct
    19381 gtgctgttgc tgttcggtgc cagctttatg gcctttatgg tgatgaaaca cgtcggcttt
    19441 agcttcaaca atctgttcag cgaagcgatg gcggtacacc cgaaaggtgt cgacatcatg
    19501 aaaccgggcg gactggtgaa agatccgatc tccgcgctct ctctggggct gggactgatg
    19561 tttggtacgg cgggcttgcc gcacattctg atgcgcttct ttacagtcag cgatgcccgc
    19621 gaagcacgta agagcgtgtt ctacgccacc ggatttatgg gctacttcta tattctgacc
    19681 tttattatcg gcttcggcgc gatcatgctt gttggtgcga atccggaata taaagacgcg
    19741 gcgggccatc tgattggtgg taacaacatg gcggccgttc acctggcgaa tgcagtgggc
    19801 ggcaacctat tcctcggttt tatttcagcg gttgctttcg ccactatcct cgcggtggtt
    19861 gcggatctga cgctggcggg cgcatcggcg gtttcgcatg acttgtacgc taacgtcttc
    19921 aaaaaaggcg cgaccgaacg tgaagagctg cgggtatcaa aaatcaccgt actgatcctc
    19981 ggcgtgattg cgattatcct cggcgtgctg tttgagaatc agaacatcgc ctttatggta
    20041 ggccttgcgt ttgccatcgc ggcgagctgt aacttcccga tcattctgct ttccatgtac
    20101 tggtcgaaac tgactacccg tggcgcgatg ctgggcggct ggctggggct gattaccgca
    20161 gtggtgctga tgatcctcgg cccgactatt tgggtacaga tcctcggtca cgaaaaagcc
    20221 atcttcccgt atgaataccc ggcgctgttc tctatcagcg tggcattcct cggcatctgg
    20281 ttcttctcgg caaccgataa ctcagcggaa ggggcgcgcg agcgtgaact gttccgcgcg
    20341 cagtttatcc gctcccagac cggctttggc gttgagcaag gccgcgcaca ttaatctctc
    20401 cccttccccg gtcgcctgac cggggaatac gcttcctctc caacatgcat caccttttcc
    20461 cataatatta aacaaataaa ctcattaaaa aatgagcgat ttttgacagt cgtagaaaat
    20521 gataatgcag agaatatgcc ttttctttct tgttaattat aaggatattt tatgcgctat
    20581 aatggtttaa ataatatgtt tttccctctt tgccagatta acgataacca ctctatcaca
    20641 agttcatcac atacaaagaa aacaaaatca gataattaca gcaaacatca taaaaacacg
    20701 ttaattgaca ataaagccct ctctcttttc aaaatagatg atcatgaaaa agtgataggc
    20761 ttgattcaga aaatgaaaag aatttatgat agtttaccat caggaaaaat cacgaaagaa
    20821 acggacagga aaatacataa atattttata gatatagcgt tatatgcaaa taataaatgt
    20881 gacgatagaa ttacgagaag agtttacctt agtaaagaaa aggatgtatc cattaaggtg
    20941 gtatatttta taaataatgt cgccgtccat aataatacta tcgaaattcc acagatagta
    21001 aatggtggtt acgatttttc acaccttagc ctgaagggta tcgtgattaa agatgaagat
    21061 ttatccaatt cgaattttgc aggttgcaga ctacaaaacg ctattttcca ggactgtaat
    21121 atgtataaaa cgaattttta ttacgccata atggaaaaaa tactttttga taattgtatt
    21181 ctcgatgact caaatttcgc tcagataaaa atggccgacg gaactctaaa tgcatgctcc
    21241 gctatgcatg ttcaattcta caatgcagca atgaatagag ccaatattaa aaataccttc
    21301 cttgactatt caaattttta tatggcgtac atggctgagg taaatcttta taaagtaata
    21361 gcgccatatg ttaatttatt taaagccgac cttagtttct ctaaactcga tttaattaac
    21421 tttgaacatg ctgatctgtc tcgcgtcaat ctgaacaaag caatcctcca gaatataaac
    21481 ttaattgata gcaaactctt ttgtacgtgg ctgacaaata cattcctcga aatggttata
    21541 tgtaccggct ctaatatggc taatgttaat tttaataatg ccaatttaag caactgccat
    21601 ttcaactgtt ctattttaac aaaagcctgt atgtttaata cccgtctcta tcgggttaat
    21661 ttcgatgagg ctagcgtcca gggaatgggc atttccattc tccgtgggga ggaaaatatc
    21721 cccattgata gtgataccct ggtaacacta cagaaattct ttgaagaaga ttgtacctct
    21781 catactggca tgtcacaaac tgaggataat attaatgcag tcgctatgaa gattactgca
    21841 gatattatgc aacacgcaga ttgaggttaa gtcgaagaac agccaatgaa ttgactttac
    21901 aagagagata acttatttgt tttagaatca tgaatgttcg cccgagctaa cctgttaaag
    21961 gtgcaaaaga ttgcaccttt ttcagattac ctcgggcctg gttacgtcgc tttcggctta
    22021 ctgattttcc tcaattagca acgcttcaag caaatcgaga tcgtgcagta atttttgcag
    22081 cgtttcgttg ctgatctccc gcgtggcgcg caggtggtac agttcagcac gttcagaacg
    22141 caatgccgcc agacggaagc gacgctcaag gttctcttcc tgcacggaac tttcaacgtc
    22201 gttacgtcca tcggcgcgac gacgcaggtt accaatgacg cgagaactga cctccgtaag
    22261 caactggtta tcgatattct cttcggtatc cgccgccaga cgctcctcca ttttctggat
    22321 cgccacaatc gccacttctg ccgttgccgc tcgcgcaata cgttcttctt tcaattgttg
    22381 cgaatggtcc gcgacttcaa tgtgttgtag cagaataggc aacatcacca cgccgacaaa
    22441 cagcgagaag agaatgacgc cagccgccag gaacaccagc tcatagcgcg ccgggaagac
    22501 gttaccatcc ggcaagagca gcgggattga gagcacaccg gccagagtga tcgccccacg
    22561 caccccagcg aaagacgcga tcaggatttc tcgtgtggtc cacgaaccaa actccatcgg
    22621 cttcttcttc aggaagcggt tgctgaactt tttcatcgtc cacagccagc cgaaacggac
    22681 cagcatcagc gccgcatata tcagaataat atcggtaaac agcatccaga tttcgacgtt
    22741 agggtcgatt tctgccgcca tcagcgacgt ctccagaata cccggcagtt gcagacctaa
    22801 cagcaggaac accatgccgt taaagacaaa ttccagcatc gcccaggtgc tgtttgcgcg
    22861 caggcgcatt gccagtggcg cacggcgcat cacaccggag cgggtgatgg tcatcccggc
    22921 ggcaaccgca gcgaggatgc ccgaaacgcc aatatgttcg gcaatcagat aagaagcgaa
    22981 tggcagcaag aacagcagaa cgatctgcgt cgcgggttca tcaccgcccc agcggctgag
    23041 gaatcgcagc gagcgaccat acagccagct caccacaaaa ccggcaagaa taccgccaat
    23101 ggctactttc ataaattcga ccgtcgcacc gccgacagtg aagatcatcg tccccatcgc
    23161 cactgccacg gcaaacttca acgacaccag gccggaagcg tcattcatca acgcttcgcc
    23221 ctgcaaaatg cccatgattt ttttcgggat gcgcccttcg ccaacaatcc cggagagcgc
    23281 cacagcatcg gtcggagaaa gtaccgccgc cagcgcaaag gcggggatca gcggaatgcc
    23341 cggcaccacc cagtaaataa ggaagccaat gccgaccacg gtgaccacca ccagcgccag
    23401 cgcgaggccg aaaatctctc gaccatgttc gagaaattca cgggtcggcg ttttccagcc
    23461 atcagcgaac agcaacggcg ggataaacaa gactaaaaag agttcaggat caaactccac
    23521 atgcaaacca aacgtcggcc acgccagtag cgcaccgatg gcgatttgca taagcggaag
    23581 cgggatctga aagggcatga cacgagtgac caccccggac agcgagacca cgagggtcat
    23641 tatcagtatg gtgaagaaga tttccatgcg ttccctgttt gcgatgtttt ttatgatctg
    23701 agaaagacgt tatgcctgaa ttctcatttt ttcagaatat cgcaaaagct cacacaatgt
    23761 gcggagaaaa taaaaacggg cagccattgc cacccgtttt cagaatccga tgaaagatta
    23821 gatagcccag ccacccgcat agaaggtcac cagcgccacg gcgataacga ccgtaccgat
    23881 gttcaacttg cgccattcac cggaaaccag acgaccaatc accagagtcg cgaagccgat
    23941 catgatgcct gttacgatgt tacaggtcag cacgatgaat accgccgtaa ccagacccgc
    24001 catcgcatca acaaaatcag caaagtcgat tttcgccacg ttgctcagca tcagcaggcc
    24061 aacgtacatc agcgccggag ccgttgcata ccccggaacg aggtaagaaa gcggagagag
    24121 gaacagaatc agcaggaaca gcacgccaac ggtgatagcc gtcaacccgg ttttaccgcc
    24181 cgccgccgta cccgccgcag actcgatgta taccgctgcc ggagctgcac ccaccaggcc
    24241 agagaaaacg ctgctcatgg agtcagtggt cagtgctttc ccgccgtcga tgatctgccc
    24301 atctttatcc agcaggttcg cctggccggc gacagcgcgg atagttccgg tggcatcaaa
    24361 tactgccgtc atcaccagcg ccagaacgct tggcaggact acaggattca gcgcgcccat
    24421 aatgtccagg ctgccaatca gtgaattgcc gttttcatcg ctcaatgaag gcatggcgaa
    24481 aacgccggag aaatggacgt taggatcgaa gatcaaaccg acaattgaga taccgataat
    24541 ggtcagcaga atgccaccag ggactttcag tttttccaag ccgatgatca ccgccagacc
    24601 tgccagtgac ataatcaccg ggaaggtcgc gaaatcaccc agcgcaacgg gcagaccatc
    24661 aagcgggttt ttaatcacca gaccgacacc attagcggca atgagcagca ggaacagacc
    24721 gataccgatc cccgtgccgt gcgccacacc gtgcggcaag ttgcgcaaaa tccagctacg
    24781 gatacccgtg gcagaaatta ccgtaaacag cacacccatc aggaacacgg cacccagcgc
    24841 gacaggtacg ctaatatgtt gccccagcac caggctgaat gcggtaaacg ccgtcaggga
    24901 gatggcgcaa ccaatcgcca acggcagatt agcccacaga cccatcacga tagaaccgag
    24961 tccggcaacc agacaggttg caacgaaaac tgccgcaggc gggaagcctg ctttacccaa
    25021 catacctgga acgacgatga ccgagtagac catcgccaga aacgttgtta acccggcaac
    25081 cacttcctga cggacagtgc ttccacgttg tgaaatttta aaccaggcgt cgagtgaacc
    25141 gccggtacgc gctgatggcg tagacataag aaaacatccc ctgagagttt aattttcgtc
    25201 aagatgcgtg gcggacaatc ccctgccagc gaaacgttat cttcctgctc caggtttgcg
    25261 gcaatttggt gcgtcgcaaa aaagcaaacg tttagctccg cgcatacaaa acgggtggta
    25321 aaaaaaagca aacgattatc cggcgatcat agcggctttt tcaaccgaag tttgctgctt
    25381 ttttctcttt tattgtcttg acgatgaaaa accaggcaat tgatgcgcaa acgttatcgt
    25441 cgatgtgcaa gattgcagcg ttatcgagcc gtataccgtt ctggattgcg ataaaaaatc
    25501 gtctgagatg acaaaaatat tgcggctaaa aagtatactt ttacactggc gaagaaacgt
    25561 cggtgacata caattaaagc atcaacacca accggaacct ccaccacgtg ctcgaatgag
    25621 gtgtgttgac gtcgggggaa accctcctgt gttccagcgg gatagagaga aagacaaaga
    25681 ccggaaaaca aactaaagcg cccttgtggc gctttagttt tgtgtttgcg gataattcca
    25741 aaagagctcg atcagtcccc tcgccccttc ggggagaggg ttagggtgag gggaacaggc
    25801 agcaccagtg caaatattta ccctcacccc ggccccatag ttaacgatga gtcagcttgt
    25861 ccttgtggcg ctttagtttt gttcatcttc cagcaagcgt gcgccggtac cttcttctcc
    25921 taagcggtcg cccgggttac gcaacgggca atcactgcgc gaaaggcagc cacaaccaat
    25981 acatccgtcc agttcgtcac gcagcgccac taaggtatga atgcgccgat ccaactcttc
    26041 tcgccattgg gacgaaagct gtttccactc tttcgcactt aacgtatgcc cttctggcaa
    26101 cacgccaaac gcttcaccaa tggtcgccag cggaatgcca atacgctgag cgattttgat
    26161 aattgcgaca tatcgcaaca catcacgttt atatcgccgc tgattgccgc tgttacggat
    26221 actagtaatc aaccctttgc tttcatagaa atgcagcgcc gataccgcca caccgctgcg
    26281 tttcgccact tcgccagggg ttagcagcgc tttaatgcgg ggtaatttct tttccataaa
    26341 tcgctttacc tcaagttaac ttgaggaatt atactcccca acagatgaat taacgaactg
    26401 aacactgaaa agaggcagat ttatgtccca tcagaaaatt attcaggatc ttatcgcatg
    26461 gattgacgtg catattgacc agccgcttaa cattgatgtc gtcgcaaaaa aatcaggcta
    26521 ttcaaagtgg tacttgcaac ggatgttccg cacggtgacg catcagacgc ttggcgatta
    26581 cattcgccaa cgccgcctgt tactggccgc cgttgagttg cgcaccaccg agcgtccgat
    26641 ttttgatatc gcaatggacc tgggttatgt ctcgcagcag accttctccc gcgttttccg
    26701 tcggcagttt gatcgcactc ccagcgatta tcgccaccgc ctgtagtttt attgcccgcg
    26761 cgttaactcc cgcgcgggta attgctccat ccattgcata aacacctgcg gcggcatcgc
    26821 cttcgcaaaa aaccatccct ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg
    26881 ctcctcagtt tcgacgcctt cagcgatcgt ttttaacccc aggctgtgcg ccagctcgat
    26941 gatgtgttcc gcaatcaaat gactggtttt gtgggtggtc agcgtttcaa caaacgattt
    27001 atcgattttc aaaatatcga cattcaatga tttaaggtta tgcaagttag agtagccaat
    27061 accaaaatca tcaattgcca cttcgtaacc agcctggcgg aaagccagaa ttaatcggcg
    27121 tcattttttc gacatcaaga aatgcatgct cagtcacttc aaatttaatt tgctgcggac
    27181 gcaccgcgta ttgctctgtt ttctgattga ttcgcgctat caaccgtgac gtatgaaaat
    27241 cggaggccga caggttaata gaaacatagc gatcggcatg tgttgccagg tacgcgccca
    27301 gatcgcggaa gacattatca ataacataat cagttatctg ttctatcatc ccctcctttt
    27361 ctgccagcgg aataaactct gccggattca ttatttgccc ctgctcacca ggccaacgta
    27421 acaacgcttc agcgccgata catttttctg ttttgatatc gattattggc tggtaataaa
    27481 gacaaagttg atgtttttcg agggcgcgtt gcaatttacg tttgggagat aaatagtttt
    27541 gtcgaatacg tagccagagt aatagcagaa caagactccc caaaataccg gcgggcaacg
    27601 caaaaataag atgattataa aaatgggtta taagacgttg atatgaagtc gcaacaatgg
    27661 ctgcaattgg gcgttttgtc gaataaattg tcgcatataa atagccattt ctttgtacgg
    27721 ttaaatcctt caaatgaatc agcggagaaa acgttgctgc cgaggcctct ttgcttaacg
    27781 agaaaaaggt tttcatcacc gtatcataca caccccattg caatgtcggg tcatcagaca
    27841 tcacttcact ccagaagaga gggttgataa ccgccacata atttccccgc tgcatatagg
    27901 tcattttata gccagagaaa aaaggcgtat cgcggtaata atagatagaa acgttaggtt
    27961 cacgcttata atcggccggt gcaatcgtat agccgtttac aggcgctatc agcgatgagc
    28021 ataaaaaatg gttatcacga attcattaat ataaagatag ccacgaataa tattcaacat
    28081 tcgcttttga tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac
    28141 taaatctgcc tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg
    28201 gagctgatta ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat
    28261 gggaaagaga actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg
    28321 actcattgct atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaccctggc
    28381 taccgacgtc tttagataaa tcttacggta taaatagcac ttttgcctga tttgaaattg
    28441 ataccttttt aatcgtcacc tggatgaaaa taattcattg atattcaaaa ttaattttct
    28501 acaggaatac agaggcggcg ggaagatatg cccttaatgc cactgaaaaa ccacataatt
    28561 tgtacagcaa taaaagtcac ggcctaatat ggctaacaaa caatcaattg tgatatagtt
    28621 cacaaaatta atgaaacaaa cagagtgttt catttttgtg ttctgcaagc cccgcgatag
    28681 ccagagtgta ccacgcctcc cgtgaacaac gccgcgctgt ccagggcatc ggctcttttc
    28741 agaggaattg tttgatggct accctcacca ctggcgtggt tcttcttcgc tggcaacttc
    28801 ttagtgccgt aatgatgttt ctggccagca cactcaacat ccgttttcgt cggtctgatt
    28861 atgtcgggct tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa
    28921 tggggttgct tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagtcgg
    28981 tgatgataga agagatgaat cagacaccgc cacaatggcc aatgattttg acctg
//
LOCUS       DAJVQR010000047        28874 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVQR010000047 DAJVQR010000000
VERSION     DAJVQR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OQM52_002180"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OQM52_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1564022.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OQM52_002181"
     CDS             complement(2138..2584)
                     /locus_tag="OQM52_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1564023.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OQM52_002182"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OQM52_002182"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1564024.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OQM52_002183"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OQM52_002183"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1564025.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OQM52_002184"
     CDS             complement(5347..6009)
                     /locus_tag="OQM52_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1564026.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OQM52_002185"
     CDS             6182..6595
                     /locus_tag="OQM52_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1564027.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OQM52_002186"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OQM52_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1564028.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OQM52_002187"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OQM52_002187"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1564029.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OQM52_002188"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OQM52_002188"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1564030.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OQM52_002189"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OQM52_002189"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1564031.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OQM52_002190"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OQM52_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1564032.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OQM52_002191"
     tRNA            complement(9861..9948)
                     /locus_tag="OQM52_002191"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OQM52_002192"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OQM52_002192"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1564033.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OQM52_002193"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OQM52_002193"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS1564034.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OQM52_002194"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OQM52_002194"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1564035.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OQM52_002195"
     CDS             14005..14592
                     /locus_tag="OQM52_002195"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1564036.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OQM52_002196"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OQM52_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1564037.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OQM52_002197"
     CDS             14984..15841
                     /locus_tag="OQM52_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1564038.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OQM52_002198"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OQM52_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1564039.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OQM52_002199"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OQM52_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1564040.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OQM52_002200"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OQM52_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1564041.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OQM52_002201"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OQM52_002201"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1564042.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OQM52_002202"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OQM52_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OQM52_002203"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OQM52_002203"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1564043.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OQM52_002204"
     CDS             complement(22884..24023)
                     /locus_tag="OQM52_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1564044.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OQM52_002205"
     CDS             complement(24069..26165)
                     /locus_tag="OQM52_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1564045.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OQM52_002206"
     CDS             complement(26165..26911)
                     /locus_tag="OQM52_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1564046.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OQM52_002207"
     CDS             complement(26908..27552)
                     /locus_tag="OQM52_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1564047.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OQM52_002208"
     CDS             complement(27659..27964)
                     /locus_tag="OQM52_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564048.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OQM52_002209"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OQM52_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS1564049.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6983 a         7315 c         7592 g         6984 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgc
//
LOCUS       DAJVQR010000048        28614 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVQR010000048 DAJVQR010000000
VERSION     DAJVQR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..638
                     /locus_tag="OQM52_002210"
                     /pseudo
     CDS             1..638
                     /locus_tag="OQM52_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            635..1021
                     /locus_tag="OQM52_002211"
     CDS             635..1021
                     /locus_tag="OQM52_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564050.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1046..3259
                     /gene="tssI"
                     /locus_tag="OQM52_002212"
     CDS             1046..3259
                     /gene="tssI"
                     /locus_tag="OQM52_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1564051.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3282..3727
                     /locus_tag="OQM52_002213"
                     /pseudo
     CDS             3282..3727
                     /locus_tag="OQM52_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3749..4156
                     /locus_tag="OQM52_002214"
     CDS             3749..4156
                     /locus_tag="OQM52_002214"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCS1564052.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4153..6441
                     /locus_tag="OQM52_002215"
     CDS             4153..6441
                     /locus_tag="OQM52_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1564053.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6438..7427
                     /locus_tag="OQM52_002216"
     CDS             6438..7427
                     /locus_tag="OQM52_002216"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564054.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7527..7730
                     /locus_tag="OQM52_002217"
     CDS             7527..7730
                     /locus_tag="OQM52_002217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564055.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7748..8068
                     /locus_tag="OQM52_002218"
     CDS             7748..8068
                     /locus_tag="OQM52_002218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564056.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8134..8366)
                     /locus_tag="OQM52_002219"
                     /pseudo
     CDS             complement(8134..8366)
                     /locus_tag="OQM52_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8476..9246)
                     /gene="yafV"
                     /locus_tag="OQM52_002220"
     CDS             complement(8476..9246)
                     /gene="yafV"
                     /locus_tag="OQM52_002220"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1564057.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9427..9873
                     /gene="ivy"
                     /locus_tag="OQM52_002221"
     CDS             9427..9873
                     /gene="ivy"
                     /locus_tag="OQM52_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1564058.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9916..12360)
                     /gene="fadE"
                     /locus_tag="OQM52_002222"
     CDS             complement(9916..12360)
                     /gene="fadE"
                     /locus_tag="OQM52_002222"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCS1564059.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12600..13178
                     /gene="lpcA"
                     /locus_tag="OQM52_002223"
     CDS             12600..13178
                     /gene="lpcA"
                     /locus_tag="OQM52_002223"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1564060.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13384..14151
                     /locus_tag="OQM52_002224"
     CDS             13384..14151
                     /locus_tag="OQM52_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1564061.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14122..14862)
                     /locus_tag="OQM52_002225"
     CDS             complement(14122..14862)
                     /locus_tag="OQM52_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1564062.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15246..15767
                     /locus_tag="OQM52_002226"
                     /pseudo
     CDS             15246..15767
                     /locus_tag="OQM52_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15943..16440
                     /gene="rayT"
                     /locus_tag="OQM52_002227"
     CDS             15943..16440
                     /gene="rayT"
                     /locus_tag="OQM52_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS1564063.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16657..18369)
                     /locus_tag="OQM52_002228"
                     /pseudo
     CDS             complement(16657..18369)
                     /locus_tag="OQM52_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18341..19126
                     /gene="lafU"
                     /locus_tag="OQM52_002229"
     CDS             18341..19126
                     /gene="lafU"
                     /locus_tag="OQM52_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1564064.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19197..20252
                     /gene="dinB"
                     /locus_tag="OQM52_002230"
     CDS             19197..20252
                     /gene="dinB"
                     /locus_tag="OQM52_002230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1564065.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20304..20597
                     /gene="yafN"
                     /locus_tag="OQM52_002231"
     CDS             20304..20597
                     /gene="yafN"
                     /locus_tag="OQM52_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCS1564066.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20600..20998
                     /gene="yafO"
                     /locus_tag="OQM52_002232"
     CDS             20600..20998
                     /gene="yafO"
                     /locus_tag="OQM52_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCS1564067.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21008..21460
                     /locus_tag="OQM52_002233"
     CDS             21008..21460
                     /locus_tag="OQM52_002233"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1564068.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21706..22749
                     /locus_tag="OQM52_002234"
     CDS             21706..22749
                     /locus_tag="OQM52_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1564069.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22811..23425
                     /locus_tag="OQM52_002235"
                     /pseudo
     CDS             22811..23425
                     /locus_tag="OQM52_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23482..24939)
                     /gene="pepD"
                     /locus_tag="OQM52_002236"
     CDS             complement(23482..24939)
                     /gene="pepD"
                     /locus_tag="OQM52_002236"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1564070.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25201..25659
                     /gene="gpt"
                     /locus_tag="OQM52_002237"
     CDS             25201..25659
                     /gene="gpt"
                     /locus_tag="OQM52_002237"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1564071.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25751..26995
                     /gene="frsA"
                     /locus_tag="OQM52_002238"
     CDS             25751..26995
                     /gene="frsA"
                     /locus_tag="OQM52_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1564072.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27053..27454
                     /gene="crl"
                     /locus_tag="OQM52_002239"
     CDS             27053..27454
                     /gene="crl"
                     /locus_tag="OQM52_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1564073.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27493..28548)
                     /gene="phoE"
                     /locus_tag="OQM52_002240"
     CDS             complement(27493..28548)
                     /gene="phoE"
                     /locus_tag="OQM52_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1564074.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7060 a         7119 c         7599 g         6836 t
ORIGIN      
        1 atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat caaaatactg
       61 gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat accgttacag
      121 atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta tctgttcagc
      181 aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc gctaaaaaat
      241 ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag aatgaagact
      301 ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat gttgatttta
      361 aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa tgtattaact
      421 gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt ggttacttag
      481 atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg atgaatgtgg
      541 gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat atcataatgc
      601 ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta ttatagtgat
      661 tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg cagttgctca
      721 atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca tcttttttat
      781 gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat ttgttggtga
      841 gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc aagatgttcc
      901 tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt ggtttcatta
      961 taaagacggg caatggagag tgattactta tattttttgg ccaccagggg ctagatttta
     1021 atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata aaccagttgg
     1081 aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg gtatgagcac
     1141 aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac aggctgcggc
     1201 ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag tagctgctgc
     1261 gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc aggatgcagc
     1321 ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg gtagtgcggt
     1381 gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg ggaatctgtt
     1441 ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg tttcccccgc
     1501 tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta tcgaactgga
     1561 cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg tcggccatga
     1621 gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc tgacccatac
     1681 cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg aagtactgga
     1741 tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc gcgcctggca
     1801 ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg aatgggggat
     1861 ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg atgccatcaa
     1921 tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag ggctgaaact
     1981 ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg gaaagtgggt
     2041 gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg tggccagtcc
     2101 gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt acttcactac
     2161 gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc caggcagccg
     2221 ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta cgttgatgaa
     2281 tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc tgtttttacg
     2341 ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca cgtttgaact
     2401 gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc acaccaaagg
     2461 cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg ataaatatgg
     2521 ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa acagttcctg
     2581 ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc agatcccgcg
     2641 tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc ccatcatcgt
     2701 ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca tggcgtcgca
     2761 gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata tgctgcgctt
     2821 cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc tcaataccac
     2881 ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca ttgtcaaaaa
     2941 tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg atggtctttc
     3001 cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg ttggcgatca
     3061 atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct cgattgagtt
     3121 ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg gcagtggcat
     3181 tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc cgttacctac
     3241 ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc ctttaccgta
     3301 ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc aatatcttcg
     3361 tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat acgctcaggc
     3421 cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa aaaaaaacct
     3481 aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg aagctgccat
     3541 gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt accagcgtca
     3601 ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca ctcagcgtcc
     3661 ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc agcctgctaa
     3721 aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg atgaccccat
     3781 ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta tcgccattat
     3841 tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga ttttgagttt
     3901 tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg ggcgctcgat
     3961 acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca acagtcgccc
     4021 ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg aaatccgcat
     4081 tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga tgaccacact
     4141 gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg cggtacgcag
     4201 acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt ggatgtgctg
     4261 tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc ggcaaagatg
     4321 gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat ggtcgggatg
     4381 gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac cactgggcaa
     4441 aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt cacctgttcg
     4501 cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg gcggctgaac
     4561 tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt acagggtcgg
     4621 gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga cccggaagag
     4681 cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac cgactgcagc
     4741 tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt tatggagacg
     4801 ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg ggtggcctcc
     4861 agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga acgcctgtcg
     4921 catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg ttacgacgat
     4981 aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca gtttgcctat
     5041 gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac ctaccactgg
     5101 gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg cgaggattac
     5161 cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc ggagcagaag
     5221 tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc gggcggcgga
     5281 atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact ccccggcgaa
     5341 cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac ggacccggca
     5401 ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg ccgggcgctg
     5461 gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat acgggagacg
     5521 gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc ggagacggtc
     5581 tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt actgagcagc
     5641 caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg gatgctggcg
     5701 cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga agggctggtt
     5761 gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg cgacggacgg
     5821 ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa tgcggacggg
     5881 tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga acagcaggag
     5941 cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg gaccttcagc
     6001 tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga aggggaacgg
     6061 ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg gtggctgacg
     6121 gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg caatcccacc
     6181 gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag cgggcatctg
     6241 ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag cctgcaccgt
     6301 caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc cgacaaccgg
     6361 ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc ggtactcccc
     6421 cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac ggtgaagtcg
     6481 gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc agcggctggc
     6541 tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac aaagcgggca
     6601 acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg ggttacgggc
     6661 gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac cagcagctgg
     6721 agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag acgcggtatc
     6781 agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat accgaccggg
     6841 gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag gagacaacgt
     6901 ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg gtggctgcca
     6961 tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg acgggcacgg
     7021 tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag gcgggatttg
     7081 gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg cagcggctgc
     7141 cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg tattatgcgc
     7201 cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg ttgaacttat
     7261 atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat tctttaggaa
     7321 tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga attggacagc
     7381 caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa aaaggtgatg
     7441 ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt ggtgcagatt
     7501 ttttctcaaa aaaacatgtc ttcaaagtgg cagtttctga tctcgatgat ttagttaaaa
     7561 ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt ttagaatcaa
     7621 gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg aataatgagg
     7681 tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag ttagtggaga
     7741 ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc cttgtttcca
     7801 tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat ctgacgattg
     7861 tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta gtgcaacgag
     7921 tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg actggagagc
     7981 taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt tttttgataa
     8041 aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat aataaccgga
     8101 aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca cactaaacat
     8161 cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc gcagttcctg
     8221 tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac ggtaacgccc
     8281 gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat caccggcgtg
     8341 tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga aatggtgact
     8401 tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc ccccggccct
     8461 aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg ccggaaactt
     8521 ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg ttgccttatg
     8581 cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt caccgcgata
     8641 atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg cctgattctc
     8701 aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg gccagttggc
     8761 gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa aacgtaagtc
     8821 gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc gcgcattgcc
     8881 cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat cataaaaatg
     8941 taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact ccgtttgtaa
     9001 tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca tccagttcac
     9061 tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc tggtaaacat
     9121 ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat caaaatggcg
     9181 caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa tcttcaaact
     9241 aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc ttatcaggca
     9301 gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat ctgatttgca
     9361 aattatcgtg ttatcgctag gctttaggag gttaataaca tgggcaggat aagctcggga
     9421 ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac cagtgcaatg
     9481 gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa agctgcgttt
     9541 aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg tacttatact
     9601 cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc gtgcaaaccg
     9661 catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa tcagatgacg
     9721 gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg gctgaatgtg
     9781 aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac cggcagcctg
     9841 gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac ggagcctttc
     9901 ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca actttaccgg
     9961 cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca gacggctttc
    10021 ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg tcagcgcgtt
    10081 gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt tacagatccg
    10141 ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta aaccaaccgg
    10201 gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag tggcgttcgg
    10261 cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca gataatgacg
    10321 tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg ggaagttttg
    10381 cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc cccagtgcac
    10441 cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa cggcagaggc
    10501 gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac gacgtttcag
    10561 gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg cgctcaggcg
    10621 gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc tggttaaacc
    10681 gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac cgatatgttt
    10741 gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca tctcttccag
    10801 cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc gggtcagaat
    10861 gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg ccaggaagtt
    10921 gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa taatcgactg
    10981 ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag gtttttcgcc
    11041 gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag cgttcccggc
    11101 aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt tgaactgacg
    11161 gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc cggttgagtt
    11221 ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc gccagccttg
    11281 cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat ctttaccgcg
    11341 cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa tttccacgcc
    11401 cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac cgccgagtaa
    11461 tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg gtgccagcgt
    11521 aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct gccattcgcc
    11581 catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt ccgggctggt
    11641 cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt gatctttctg
    11701 ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg gcacgccgac
    11761 ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag actgggcata
    11821 agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga agcgatgctc
    11881 tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa tctggaaatc
    11941 attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct cttcagcggt
    12001 caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct ggaacaagtc
    12061 gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg acatcggcgg
    12121 catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac gcataggcgc
    12181 aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg accacagacc
    12241 agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa ataagctcac
    12301 gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca aaatcatcat
    12361 aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg ttgtagtgga
    12421 tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac attgttgctg
    12481 tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt acttctcgct
    12541 tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag gatatcctca
    12601 tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg gctaactttt
    12661 taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta gcagacagct
    12721 ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc gacgctatgc
    12781 actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac ccggcgattg
    12841 ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc aatgatattt
    12901 tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg atctccacct
    12961 ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag gggatgaaag
    13021 tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat atcgaaattc
    13081 gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa gtgatccata
    13141 tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg ccggataagg
    13201 cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga cgcgtcttat
    13261 catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc cgcatccgac
    13321 ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt gtggaggtta
    13381 cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg ctttagtttc
    13441 accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg gggcattacc
    13501 ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt taattccccc
    13561 atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc tcatattcgc
    13621 caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg cgagttatgg
    13681 gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc actggaaacc
    13741 ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg gctgctgcat
    13801 aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt taaatatatc
    13861 gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct ttcggacggg
    13921 cgctatgtaa tggcgtattg ctcgactaat ttacactgga tcacccgccg cgcgccgttt
    13981 ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca gaccacaccg
    14041 aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac ctggcaaaaa
    14101 attatgccag gcgaatggcg cttattttgc ctcggggagc gtgtagtttg atgccagttg
    14161 tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat tggccacaga
    14221 aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca gctgctccca
    14281 gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca tgcggaatgg
    14341 gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa cgaactggaa
    14401 gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac aatcgccgtg
    14461 gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg ggaatccaat
    14521 attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga cgctataaaa
    14581 cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc cggaatattt
    14641 acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt aaagatcgag
    14701 cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact gctgcttata
    14761 ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa acgaaacgca
    14821 cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta ttccttgata
    14881 aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt ctggatttgg
    14941 aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg gtttacaaat
    15001 ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat ttcctaatat
    15061 tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta ctcctccggt
    15121 cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc gttcttgctc
    15181 ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt actcgccagc
    15241 gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg ccgctcaagg
    15301 cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc agcgatcacc
    15361 tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc aagccttacg
    15421 tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt ttttatgcct
    15481 acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac cactaccgtc
    15541 gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt ttccatatcc
    15601 acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag tttattgagt
    15661 cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc tggcaggacc
    15721 attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc ggataaggcg
    15781 tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg cgtcttatca
    15841 tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag cgtcattgtc
    15901 ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga atatcgtcgt
    15961 tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg tcgaagccat
    16021 cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa gcgagacaga
    16081 ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat atggacatta
    16141 cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca gttcacccat
    16201 gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat tcgcaataca
    16261 aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca tggctgggta
    16321 aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg agggttatat
    16381 cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat cattttataa
    16441 ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg ctttaacggt
    16501 aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct gatgcgacgc
    16561 tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat aaggcgttta
    16621 cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc ccataatctt
    16681 caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg gcgcaaacaa
    16741 ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca ccggatcttt
    16801 ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact ggttgagcat
    16861 attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg cctgattcac
    16921 tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca gctcctgctt
    16981 gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg ccgccagcag
    17041 aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg tgacgatatc
    17101 gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt ggctgtaatt
    17161 gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac ggttatgcaa
    17221 ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa tcttgttcac
    17281 atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca gagccttcgc
    17341 tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt tccccagcac
    17401 gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat ccgccggaat
    17461 atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg gcttgaggcg
    17521 gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg aaatcacctg
    17581 ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca ccagcgccac
    17641 cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt cccaactgac
    17701 ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt tctcttccgc
    17761 ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca gggcgctgaa
    17821 cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca tgatcccggt
    17881 ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat gggcgatatc
    17941 gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca gcagggaagg
    18001 gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga aggcggcgtc
    18061 ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt tgatcgccag
    18121 aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt ccatcgcgcc
    18181 gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg cctggttgat
    18241 caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg cgtccagggt
    18301 aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga taaagttgat
    18361 tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc acgggcagtc
    18421 gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc atccgccaaa
    18481 acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg tgaatgagaa
    18541 aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa ccagtatcaa
    18601 caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg ttccccaggg
    18661 attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg gcagcgcgca
    18721 gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg actcgctcta
    18781 taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca atatctacaa
    18841 caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg aagaggccgg
    18901 aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc tgctggacgc
    18961 gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga ccaaaagtgc
    19021 gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc agccgctggt
    19081 gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc tgaatcttta
    19141 cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg tgagcaatgc
    19201 gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg cgcgacaatc
    19261 ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg ggggtgatca
    19321 gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct actgggatgg
    19381 cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc tacaaagaag
    19441 cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa ccgttgtcac
    19501 tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct gcgaccctca
    19561 tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca tctgcgggcg
    19621 tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc aacggtcagt
    19681 ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg gaaaaaatcc
    19741 ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg acctgcggtg
    19801 atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt ggccgcattt
    19861 tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg ttacgaaaat
    19921 ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa tgtgaagcga
    19981 ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa cctgatttac
    20041 tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc acccaggagc
    20101 acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa acctgggatg
    20161 aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg cttgacccgc
    20221 aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata ttctggtgtg
    20281 cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg gtcaatatca
    20341 ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg gttctttcta
    20401 ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta atggacatgc
    20461 ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt gctgcaagat
    20521 tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat gatgatttca
    20581 atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc tgcaacttac
    20641 agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg acggtgtttt
    20701 gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc cattaatcaa
    20761 atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc cgccacagtt
    20821 gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg gtgcgtttga
    20881 tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac tggctcgaga
    20941 taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc gtttttgaat
    21001 ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc agcaactatg
    21061 cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac tacaacaaat
    21121 tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga aatcacaagt
    21181 gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg aacactatat
    21241 cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg ctttgttaaa
    21301 accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg caaaaccctt
    21361 ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc ggggagcgtg
    21421 gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg caatgaaaat
    21481 aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag gtaaatcatg
    21541 acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt tttttcttgc
    21601 cctggattcg tctgccattt cctgattttt taattgttac tctcaataat tcaagtcgca
    21661 gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg caattatatt
    21721 cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat cgagagttta
    21781 gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt agtggggatg
    21841 ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc cgcaggccgt
    21901 ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg gcaaacagat
    21961 attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc tttggatgac
    22021 gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca gcatccgtgg
    22081 cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca acaagttgat
    22141 caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca tctgcaactg
    22201 ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca tattgcctcg
    22261 ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc gcgttttaat
    22321 cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag tccggttctg
    22381 gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg ggtcttacat
    22441 cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc acgcggtgat
    22501 tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct ggcgcatggg
    22561 gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa atacacagcg
    22621 acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa acaactcatc
    22681 ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg tcagggcagg
    22741 ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa aacagtggag
    22801 ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag aatgttgtct
    22861 cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag acgtcgcggt
    22921 aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg cgctgatttc
    22981 tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta ttcagtggat
    23041 ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg gcattgggcg
    23101 ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc tgcgttcgtc
    23161 ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatttggc
    23221 atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca aacggctggc
    23281 gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg cggtgctgaa
    23341 atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac gaacgtttac
    23401 agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc
    23461 atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag
    23521 tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt
    23581 gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca
    23641 ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc gctgataggt
    23701 ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag gatatgcgcc
    23761 tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc tcaccacgta
    23821 gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt tattgtcagt
    23881 catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca cgtcggaatt
    23941 gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc
    24001 aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat ttttctcttt
    24061 ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt tcagtgcgtc
    24121 gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag
    24181 tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac ccgccaggaa
    24241 gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc cggagtgacc
    24301 gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg
    24361 atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt agatttcacc
    24421 ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc
    24481 gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca gcgggccgtg
    24541 aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc cgttatccgc
    24601 acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc
    24661 tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg
    24721 taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa tcaggatatt
    24781 acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc
    24841 agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc
    24901 ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca
    24961 cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg
    25021 cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
    25081 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
    25141 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
    25201 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
    25261 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
    25321 gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
    25381 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
    25441 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc tgttgcgatt
    25501 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
    25561 ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca gccgtgggat
    25621 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcattg
    25681 ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa gtattctgga
    25741 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
    25801 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
    25861 ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
    25921 ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg tgatgagcga
    25981 tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg gtggcaactg
    26041 gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta cggaagaaga
    26101 tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca ttgccgccta
    26161 tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa accgcgccta
    26221 tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta ccgtacccgg
    26281 cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt tcccgacagt
    26341 attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt atgaacgtta
    26401 ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg gcttttcttc
    26461 aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa aggcgctgcc
    26521 taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa
    26581 cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg cctgtcttgg
    26641 tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc cggaaatgta
    26701 tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag cgttacgcgt
    26761 ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct gcccaacgcc
    26821 aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact cacgcttaat
    26881 cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg tgtatcggaa
    26941 ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt gttaaaaatt
    27001 tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag caatgacgtt
    27061 accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag gcccgtatat
    27121 tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa
    27181 cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc tggaagcgca
    27241 ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag gcgactggaa
    27301 gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt
    27361 tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac cggcgaatga
    27421 ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat gcatcacatc
    27481 cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat cattattaat
    27541 attcagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg ctgacatatt
    27601 tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt catcaccgat
    27661 gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca gaccaaagtc
    27721 aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc cgccagttat
    27781 tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat tggcatcgta
    27841 tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt gcaggttctg
    27901 ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc tgccgccaaa
    27961 gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt cgcggttttc
    28021 gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga agaagtcggt
    28081 gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg tctgcgccga
    28141 ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat acaacgcgcc
    28201 caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg caaaagcgag
    28261 acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact ccgcttccca
    28321 ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac caaaacggat
    28381 ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg ctttaacttt
    28441 gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct gtacagatgc
    28501 agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga
    28561 ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagt
//
LOCUS       DAJVQR010000049        28412 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVQR010000049 DAJVQR010000000
VERSION     DAJVQR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            566..1336
                     /locus_tag="OQM52_002241"
     CDS             566..1336
                     /locus_tag="OQM52_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1564075.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1327..1800
                     /locus_tag="OQM52_002242"
     CDS             1327..1800
                     /locus_tag="OQM52_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1564076.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1907..2446
                     /gene="dnaT"
                     /locus_tag="OQM52_002243"
     CDS             1907..2446
                     /gene="dnaT"
                     /locus_tag="OQM52_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1564077.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2449..3186
                     /gene="dnaC"
                     /locus_tag="OQM52_002244"
     CDS             2449..3186
                     /gene="dnaC"
                     /locus_tag="OQM52_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1564078.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3235..3729
                     /locus_tag="OQM52_002245"
     CDS             3235..3729
                     /locus_tag="OQM52_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1564079.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3969..6260
                     /gene="opgB"
                     /locus_tag="OQM52_002246"
     CDS             3969..6260
                     /gene="opgB"
                     /locus_tag="OQM52_002246"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1564080.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6398..7420)
                     /gene="lgoD"
                     /locus_tag="OQM52_002247"
                     /pseudo
     CDS             complement(6398..7420)
                     /gene="lgoD"
                     /locus_tag="OQM52_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7559..8473
                     /locus_tag="OQM52_002248"
     CDS             7559..8473
                     /locus_tag="OQM52_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1564081.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8688..10049
                     /locus_tag="OQM52_002249"
     CDS             8688..10049
                     /locus_tag="OQM52_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564082.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10098..11762)
                     /gene="tsr"
                     /locus_tag="OQM52_002250"
     CDS             complement(10098..11762)
                     /gene="tsr"
                     /locus_tag="OQM52_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1564083.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11881..12327)
                     /gene="hpaR"
                     /locus_tag="OQM52_002251"
     CDS             complement(11881..12327)
                     /gene="hpaR"
                     /locus_tag="OQM52_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1564084.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12599..13888
                     /locus_tag="OQM52_002252"
     CDS             12599..13888
                     /locus_tag="OQM52_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1564085.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13885..15351
                     /gene="hpaE"
                     /locus_tag="OQM52_002253"
     CDS             13885..15351
                     /gene="hpaE"
                     /locus_tag="OQM52_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1564086.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15353..16204
                     /gene="hpaD"
                     /locus_tag="OQM52_002254"
     CDS             15353..16204
                     /gene="hpaD"
                     /locus_tag="OQM52_002254"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1564087.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16214..16594
                     /locus_tag="OQM52_002255"
     CDS             16214..16594
                     /locus_tag="OQM52_002255"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1564088.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16662..17465
                     /gene="hpaH"
                     /locus_tag="OQM52_002256"
     CDS             16662..17465
                     /gene="hpaH"
                     /locus_tag="OQM52_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1564089.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17476..18279
                     /gene="hpaI"
                     /locus_tag="OQM52_002257"
     CDS             17476..18279
                     /gene="hpaI"
                     /locus_tag="OQM52_002257"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1564090.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18301..19656
                     /gene="hpaX"
                     /locus_tag="OQM52_002258"
     CDS             18301..19656
                     /gene="hpaX"
                     /locus_tag="OQM52_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1564091.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19666..20556
                     /gene="hpaA"
                     /locus_tag="OQM52_002259"
     CDS             19666..20556
                     /gene="hpaA"
                     /locus_tag="OQM52_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS1564092.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20583..21520
                     /locus_tag="OQM52_002260"
                     /pseudo
     CDS             20583..21520
                     /locus_tag="OQM52_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21747..23305
                     /gene="hpaB"
                     /locus_tag="OQM52_002261"
                     /pseudo
     CDS             21747..23305
                     /gene="hpaB"
                     /locus_tag="OQM52_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23323..23835
                     /locus_tag="OQM52_002262"
     CDS             23323..23835
                     /locus_tag="OQM52_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1564093.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24228..26378
                     /gene="btsT"
                     /locus_tag="OQM52_002263"
     CDS             24228..26378
                     /gene="btsT"
                     /locus_tag="OQM52_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1564094.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26428..26631
                     /locus_tag="OQM52_002264"
     CDS             26428..26631
                     /locus_tag="OQM52_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1564095.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26642..27598
                     /gene="yjiA"
                     /locus_tag="OQM52_002265"
     CDS             26642..27598
                     /gene="yjiA"
                     /locus_tag="OQM52_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1564096.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27667..>28412)
                     /locus_tag="OQM52_002266"
     CDS             complement(27667..>28412)
                     /locus_tag="OQM52_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCS1564097.1"
                     /translation="LENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTEDFNL
                     DDFSQFITAFEKVYMHGWLKKRIKSQREMVCYSALVAINNDMPFDSVINQINQHADNS
                     GFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNE
                     YWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKD
                     VCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6951 a         7428 c         7126 g         6907 t
ORIGIN      
        1 tccttaaatg tttccagcaa attaatagat taagcgaata aattatcctt agcattcact
       61 ttcatatcag catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat
      121 caaatgataa cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag
      181 aaatacttaa tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt
      241 cccgcagaat gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac
      301 ttgttaacac taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaacaa
      361 taaattcatt atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc
      421 tttttatcac cgagcttgtc ccaataacca ggattaatca gcgcaatttt gatcttaacc
      481 taccgcaaaa caaccgtgct tcatgataca aatggattct cattatctga acaatacgca
      541 gcacgtctat gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct
      601 ttgtatccag tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga
      661 gctttcctca cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc
      721 gaacgccata gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa
      781 tcacgatcgc ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc
      841 ggagcatcat ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt
      901 acgttaccca agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa
      961 actcaataac ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat
     1021 gtatatccgc cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac
     1081 cgctttcgcc gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac
     1141 ccccaccatt gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa
     1201 tgccgtcgcc gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc
     1261 cagtctgctg acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct
     1321 acgcggatgg gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc
     1381 attcctgcgg tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt
     1441 gcgctgcttg gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat
     1501 attgagtggt caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg
     1561 cgctggtatc tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg
     1621 ggcatatcgg cttataccgc aatgatttcg tcggtaaaaa tcagccagtt aggttacagc
     1681 gaaccgttga tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg
     1741 ttatccatcg gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa
     1801 aattgcctcg cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata
     1861 aaagttatct cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt
     1921 tttgaccccg gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc
     1981 aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt
     2041 cacgcctgca cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag
     2101 cgatgtcgct ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc
     2161 catggggaaa ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc
     2221 ggcgctatgg ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat
     2281 tgcctactgg caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc
     2341 gcgcagcctg caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac
     2401 ggtcagtgaa cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt
     2461 ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa
     2521 acgggtgaag agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc
     2581 gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca
     2641 ctgcatcaga actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg
     2701 ttaagcaaag cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt
     2761 tctggtaagc caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg
     2821 ctacgcggta aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat
     2881 accttcagga atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat
     2941 ctgctggtga tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc
     3001 aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt
     3061 aatatggaag agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt
     3121 aacagtttat gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag
     3181 tattaatccc cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg
     3241 aaaactatta aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg
     3301 gtgcatgctg cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa
     3361 aacagcacga cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc
     3421 ggaaaccaag ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc
     3481 gcgaagcaaa agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa
     3541 aagctgggcc tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag
     3601 ggtttgctga aaacaaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg
     3661 ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc
     3721 atttcctgaa ccgcgaaatg ttacccggct gacgcggctc tctcctgccc ttttccctgc
     3781 tcctggacgg ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat
     3841 aaactaattc ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg
     3901 ccattacatt gtgcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg
     3961 atcgcccatt gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg
     4021 catggaaagc gggacgtaac acctggtggt ttgcagccac gttaacggtg ctggggctat
     4081 ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg
     4141 acgcggttct ctatacctta accaacagcc tgaccggtgc tggcgtcagc aaatacatcc
     4201 tgccgggtat cggcattgtg ctggggctga cggcggtgtt cggtgcgctg ggctggatcc
     4261 tgcgccgtcg tcgtcatcat ccgcaccatt ttggttacag cctgctggcg ctcttactgg
     4321 cgctgggttc ggtggacgcc agcccggcat ttcgtcagat aacggaactg gtgaaatccc
     4381 agtcacgcga tggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc
     4441 ctgacccgaa actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg
     4501 ataacgaagc tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg
     4561 atttcagcca cacgcagcag ctgccaggaa cggattacac gattgcgggc atggtggctt
     4621 ctcagtgcgg cataccgctg ttcgccccct ttgaaggcaa cgcctccgcc tctgtctcca
     4681 gcttcttccc gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact
     4741 atttcgtgca gggcgcaaat ctgcgttttg ccggtaaaga tgtgttcctg aaatcgcacg
     4801 gcttcgacca cttatacggc tcagaagagc tgaaaagcgt ggtagccgac ccgcactatc
     4861 gcaacgactg gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag
     4921 agctttcccg ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc
     4981 cggatggttt tatctcgcgc acctgtaacc gcaaaaaata tgattttgac ggtaagccga
     5041 atcagtcatt cagcgcggta agttgcagcc aggagaatat cgcgacgttt atcaacaaaa
     5101 tcaaagcgtc accgtggttt aaagatactg tcatcgtcgt ctcttctgac catttagcga
     5161 tgaacaacac ggcgtggaaa tacctcaata agcaggatcg caataacctg ttttttgtca
     5221 ttcgtggcga caagccgcag caaaagacgc tggcagtgaa gcgtaacacg atggataacg
     5281 gcgcgacggt gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt
     5341 tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga
     5401 agccagatat catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc
     5461 agcagaaaaa catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg
     5521 tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata ctcagcaccg ctgcgtttcc
     5581 agctggccga tttcgctcca cgcgacaatt tcgtctgggt tgacagttgc tacaagatgg
     5641 cacaactctg ggctccggaa ctggcacttt ccaccgactg gtgtgtctcg caggggcagc
     5701 ttggcggtca gcaaattgtt cagcatgttg acaaaacaac atggaagagc aagacggcat
     5761 ttaaagatac ggtcatcgac atggcgcgtt acaaaggcaa tgtcgatacg ctgaagattg
     5821 ttgataacga tattcgctac aaagccgaca gtttcatctt taacgtcgcc ggtgcgccag
     5881 aagaggtgaa acagtttagc gggatttccc gtccggagtc gtggggccgc tggtccaacg
     5941 cgcagctggg cgatgaagta aaaatcgagt acaagcatcc gctgccgaag aaatttgacc
     6001 tggtgattac cgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag
     6061 gcaatgaaga acaaactctt gtgctgggca atgaagtgac caccaccaca ctgcatttcg
     6121 ataacccaac cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg
     6181 aagggaatat cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag
     6241 tggtagaacg tgaagggtaa ttcctgactg atgtgtaggt cgcatagggt gcgacctaca
     6301 cgccttctcc ggcttacaac acattattaa gaaacaaaaa aaacagcgcc caacacttcc
     6361 ccggacgctg catggcaact ttaataatct cgccttatca gaaagtaatt acacccttaa
     6421 tcaactcacg attgttaatc acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat
     6481 agcgatgggt taacatcatg tcggcagtga ttttcccttc cgccatcagt cgaccgactt
     6541 tagcgaaatc ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct tttttatgga
     6601 attccggatc ggagaactgc aactcacctt taaacagacc gacaaatacc accgtgccgc
     6661 cgtgacgaat cagattcacg gtattattca tcgcatgttg attacctgtc gcgtcgatca
     6721 ctttctgcgc cagcgaacca ccaaactgcg cccgcagctg cgcgtcaaaa tcttcggctg
     6781 acgggtccag cacgggcaac tccagacgcg ttgccacatg ttcacggcgc gccggactgg
     6841 tatccgccac caccacctgt gcgccatcgg ctttagcgat tgccgccgcg cccagaccga
     6901 ttggccccgc cccgaccacc agcacctgct cgccgggagc aatggctgcg cgacgcaccg
     6961 catgagcgct aatagcgaaa ggttcaatca atgcggccgc ctgcgggtca ataccgtctg
     7021 cgggcaaaat gttcgccacc ggcacgctca aatactcact aaaaccgcca tcctgatgca
     7081 cgccaatgac tgaaattttt tcacagcagt tggtacgccc gcttttacac gccgggcatt
     7141 gctgacaggc aacataaggg atcacagcaa cttgctgacc atttttaaga tcagcaatat
     7201 ttttacccag cccaacaatc tccccacata tttcatggcc taaaacccgt ggataactaa
     7261 aaaatggttg atttccaccc taggcatgaa tatcggtacc gcaaatcccg acagacttaa
     7321 tttttattaa tgcttcattg tcacccggaa taggtatctc gcgttgtttc cagactaatt
     7381 ctttcggctg ctggcaaatt aaaacattca tcgtagacat gattcggcta tccatttttt
     7441 gttgccttag atatcaatga aaatgaaaaa tggcagcgga acggaaaatc tgttttgtga
     7501 aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg actcacaagg aggaaaatat
     7561 gagtcgttca caaaatttac gccacaatgt gattaaccag gtcattgatg atatggcgcg
     7621 gggtcatatc ccttccccac ttccatcgca aagtgcgctg gcggaaatgt acaacatcag
     7681 ccgcactaca gtgcgtcata ttctcagcca attacgcgaa tgcggcgtct taacgcaggt
     7741 tggaaacgac tatgttattg cgcgtaaacc tgaccatgat gacggtttcg cctgtactac
     7801 cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct tttttcacga tgataaacca
     7861 gcgtcagtta cgccctaggg aaactttttc tgaactgcaa ctggctcggg ccgcgggtgt
     7921 cagcccggtg gttgtaagag aatatctttt aaaattcggc cgttataatc ttattcaaag
     7981 cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa tcctacgccg agcagctgtt
     8041 tgagctacgc gaaatgctcg aaacacattc acttcagcac tttctcaacc tgcctgatca
     8101 tgatccccgc tggctgcagg cgaaaaccat gctagaacgt catcggctac tgcgtgacaa
     8161 catcggcaat agctttcgca tgttctcgca acttgacaga gattttcact ccttactgct
     8221 ttcagctgcc gacaatatct tttttgatca atcgcttgag atcatctcag tcatctttca
     8281 ttttcactat caatgggacg aaagcgatct taaacagcgc aatatcattg ccgtcgatga
     8341 acatatgacc attctcagcg ctctgatttg ccgcagtgac cttgatgcca cccttgcgct
     8401 ccgtaaccat ttgaattcag ctaaacaatc aatgattcga tccattaatg agaacacgcg
     8461 ttatgctcat taagtacgga ttaaaaacag caaactatta gcgggtgcac ggttttatca
     8521 aaagataaat accgggaaat tcccgcttac ctatgctcac aatcagtcga ctaaaaatgt
     8581 ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc gttaatgtca accttgcgta
     8641 atgcacaatt acgtaaacaa ttaaaattat aaatacagga gtccggcgtg gaaaaagaaa
     8701 atatcaccct cgatccgcgt tcttcattta ctccatcttc gtcggcagat attcccgtgc
     8761 caccagatgg attagttcaa cgcagtaccc gaattaaacg cattcaaacc accgccatgt
     8821 tgttattatt ttttgcggcg gtaatcaatt atctcgaccg cagttcgctg tcggtagcaa
     8881 atttaacgat tcgtgaagaa ttgggattaa gtgccaccga aatcggcgct ttgctctccg
     8941 tgttttcact cgcttacggc attgcgcaac ttccttgcgg cccactattg gatcgtaaag
     9001 gcccacgcct gatgctggga ctggggatgt tcttctggtc actgttccag gccatgtctg
     9061 gcatggtgca cagctttacg cagttcgtgt tggtgcgtat cggtatgggg attggtgaag
     9121 cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg gttcaacatc aaagagcgcg
     9181 gacgcccgat gggcttcttc aacgcagctt ctaccattgg cgttgctgta agcccaccga
     9241 ttctggcggc aatgatgctg gtgatgggct ggcgcgggat gtttattacc attggtgtac
     9301 tggggatttt tctcgccatc ggctggtata tgctctatcg caaccgcgag cacgtagaac
     9361 tgactgccgt tgaacaagct tatctcaatg caggtagcgt caatgcccgc cgagatccgc
     9421 tcaattttgc cgaatggcgc agcctgttcc gtaaccgcac aatgtgggga atgatgctcg
     9481 gattcagtgg catcaactac actgcgtggc tgtatctggc ctggcttcct ggttacctgc
     9541 aaacagccta taacctggat ttaaaaagca cagggttgat ggcggctatc cctttcctgt
     9601 ttggggctgc cgggatgctg gtcaacggtt acgttactga ctggctggtc aaagggggaa
     9661 tggctccgat taaaagccgt aagatctgca ttattaccgg gatgttctgt tctgccgcct
     9721 ttacgctgat agtaccacaa gcgacaacat ccatgatggc ggttctgctg attggcatgg
     9781 cactgttctg tattcacttt gccggaacat cctgctgggg cttgatccac gtcgcagttg
     9841 cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc atctgcgcct
     9901 ctttcgcgcc gatcattact ggttttattg ttgatacaac ccactcattc cgtctggcac
     9961 taatcatttg cggttgcgtc accgcagcgg gagcactggc gtacatcttc ctggttcgcc
    10021 agccgatcaa cgacccgcgt aaagattaaa ctccctatgc ccgataacat tttgcttatc
    10081 gggcattttt atggcgatta aaacgtttcc cagttctccc cgctatctgc cactgccatt
    10141 ttacgcggcg tagctggcgt cacggttttt accacagccg atgtttcacg ctgctgttgc
    10201 tgctgttgct gaatccggaa cactgccaca gcttcggtca ggcgactggc ctgctcttcc
    10261 agcgcggcgg cggcagcggc agattcttcc accagcgcgg cgttctgttg agttacccgg
    10321 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10381 gaagctattt cgcccataat gtccgtcacg cgggtcacag cactgacaat ctccgccatt
    10441 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10501 atcaggcttt taatttcacg aaccgcctgg gcgctacgct gggccagatt acgcacttct
    10561 cccgcaacca ccgcaaaacc acgtccttgc tcacccgcac gcgctgcttc aaccgccgcg
    10621 ttcaaagcca gaatattggt ctggaaggta atgccgtcaa ttacgctgat aatatcggcg
    10681 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg
    10741 ccgccgcgtt gcgccgtttc agaagcactt aatgccagat ggctggcctg gcgcgcattc
    10801 tcggcgttct gtttcaccgt tgcggtcagt tgctccatgc tggctgccgt ctcttccagc
    10861 gaagcggcct gttgctcggt gcgcgaagag agatcattat tgccggtagc gatttcgctg
    10921 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    10981 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    11041 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    11101 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    11161 atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg
    11221 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11281 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt
    11341 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11401 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc
    11461 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11521 atattattct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11581 aacaacgcga cccagctgcc attcagcgtg ggttgctgct ggcgaatggt ttgtaaaaca
    11641 gtgaaatttt ctttgtcatt ctttaaggca ttaaaaaaca gaccgcctga tgtcagttgt
    11701 aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11761 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11821 gaaaaggtgt aacccgccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    11881 ttattcatta tcgcccggga tgtcttcttg tcgggaattg cccagagcaa taaattcctc
    11941 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    12001 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggtcagcga
    12061 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    12121 catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    12181 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12241 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12301 caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat
    12361 atagaaacaa ttaatcatca cgttaccggg actgagctgc ccccgcaatt cttcatttta
    12421 tcaaaattga tgatatttta ttaacttact gatatttaat attttattat ctttaaatgt
    12481 aaatagtttg ttaattagat cacatttata tcacttactt ttgccaaact acgcaatcgg
    12541 gaataaaagt aatcattaac atattaatga ttaagatgat cgaatccgag gagtggtaat
    12601 gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg cctggcagga
    12661 agcgttccag caatctccct acaaagcccc gcctaaaact gcggtctggt ttattaaacc
    12721 gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg aaaaagtact
    12781 gagcggtgcg actgttgcgc tgattgtggg gaaaacggcg acgaaagtac gtgaagaaga
    12841 tgcggcagag tacatcgccg gatatgcgct ggataacgac gtcagcctgc cggaagagag
    12901 cttttaccgt ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca ttggcgaaac
    12961 cgtggctctc agcaatgtcg ataatctgac tatctatacc gagatcaacg ggcgtcctgc
    13021 cgatcactgg aataccgccg atttacaacg taacgccgca cagctgctga gcgccctgag
    13081 cgaatttgcc acgctgaatc caggcgatgc tattctgctc ggcacgccac aggcgcgcgt
    13141 ggaaatacag ccaagcgatc gcgtgcgtgt tctcgcagaa ggtttcccgc cgctggaaaa
    13201 tccggtagtg gacgaacgtg aagtgaccac gcgcaagagc ttcccaacgc tgccacaccc
    13261 gcacggtacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg aactggaatt
    13321 taagccaccg gaagagccgc tggtgttcct gaaagcgccg aataccctca ctggcgacaa
    13381 ccagacctcc gtgcgcccga acaatattga atacatgcac tacgaagcgg agctggtggt
    13441 agttattggc aagcaggcgc gtaacgtcag cgaagccgat gccatggatt atgtcgcggg
    13501 ctacaccgtg tgtaacgact acgccattcg cgactatctg gaaaactact accgccctaa
    13561 cctgcgggtc aaaagccgcg acggactgac gccgatgctt tcaaccatcg tgccgaaaga
    13621 ggcgatcccg gacccgcata atctgaccct tcgcaccttc gtcaacggcg agttacgcca
    13681 gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct acttaagcga
    13741 atttatgacc ctgaatccgg gcgacatgat cgccaccggc acaccaaaag gcttatctga
    13801 cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc tggtgaaccg
    13861 aattgtgagt gaggaaacag cgaaatgaaa aaagtaaatc attggatcaa cggcaaaaat
    13921 gttgcaggta acgactactt ccagactacc aatccggcaa cgggtgaagt gctggcggat
    13981 gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa agaggcgttc
    14041 ccgaaatggg ctgatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg tctgggcgat
    14101 ctgatcgacc agaacgtgcc tgagatcgcc gcgatggaaa ccgcggacac gggcctgccg
    14161 atccatcaga ccaaaaatgt gttgatccca cgcgcttctc acaactttga atttttcgcg
    14221 gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat gctcaactac
    14281 acgctggtgc aaccggtagg cgtttgtgcg ctggcgtcgc cgtggaacgt gccgtttatg
    14341 accgccacct ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt gctgaagatg
    14401 tcggaactct ccccgctgac cgccgaccgc ctgggtgagc tggcgctgga agctggtatt
    14461 ccggcgggcg tgctaaacgt ggtacagggc tacggcgcaa ccgcaggcga tgcgctggtc
    14521 cgtcatcatg acgtgcgtgc cgtgtcgttc accggcggta ccgccaccgg gcgcaacatc
    14581 atgaaaaacg ccgggctgaa aaaatactcc atggagctgg gcggtaaatc accggtgctg
    14641 atttttgaag atgccgatat tgagcgtgcg ctggacgccg ccctgttcac catcttctca
    14701 atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcagag catctatcca
    14761 gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga tccgaacgat
    14821 ccgaataccc aggttagggc gcttatcagt cagcaacact gggaaaaagt ctccggctat
    14881 atccgtctcg gcattgaaga aggcgcaacc ctgttggcgg gcggcccgga taaaccgtcc
    14941 gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caacggtgct ggcggacgta
    15001 gataaccgta tgcgcgttgc ccaggaagag attttcgggc cggtcgcctg cctgctgccg
    15061 tttaaagacg aagccgaagg cttacgcctg gcaaacgacg tggagtacgg cctcgcgtcg
    15121 tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat tgaagcaggc
    15181 atggtgttcg tcaacaccca gaacgtgcgc gacctgcgcc agccatttgg cggcgtaaaa
    15241 gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc ggaaatgaag
    15301 aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg atatgggtaa
    15361 gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatctctctg aactaccagg
    15421 aaaaaaccac ggttgtcgcc agggtgcgat cgacgggcat aaagagatca gcaagcgttg
    15481 ccgggaaatg ggcgtcgata ccattatcgt tttcgatact cactggctgg tcaacagtgc
    15541 ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg agctgccgca
    15601 ttttattcgc gacatgacct acaactacga gggcaacccg gagttggggc agctaattgc
    15661 cgaggaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aatattccca gcctgaaact
    15721 ggaatacggc acgctggtac cgatgcgcta catgaatgaa gacaagcact tcaaagtggt
    15781 ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc tgggcgaagc
    15841 gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt
    15901 atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga
    15961 gttcgaccgc cagatggacg agcgtgtggt gaagctgtgg cgcgaaggcc agttcaaaga
    16021 gttttgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga
    16081 cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat
    16141 taccgagctg ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgctgcccgc
    16201 gtaaggacgt tttatgccgc actttatcgt ggaatgtact gaaaatattc gtgaagaagc
    16261 ccgcttacct gagctgtttg ccagtgttaa cactgcactg gcggcaacgg ggatttttcc
    16321 gcttggggga atacgtagcc gggcgcactg gatcgatacc tggcaaatgg ctgatggcaa
    16381 gcacgattac gcctttgtgc atatgacgct aaaaatcggc agcggacgca gtctggaaag
    16441 ccgtcaggaa gtgggcgaaa tgctgtttga tctgattaaa acgcacttcg cctcactgat
    16501 ggagagccgt tacctagcgc tttcatttga gattgcagag ctgcacccga cgttgaattt
    16561 caaacagaac aacgtacacg cgttgtttaa ataaatttgc accagcttcc aggccggatg
    16621 ataaggcgct gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacacaccc
    16681 acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga
    16741 tctcgctgga ttacccggag atcaccatcg aagacgccta cgccgtgcag cgcgaatggg
    16801 ttcgcctgaa aatcgccgaa agtcgcacgc tgaaaggcca caaaatcggt ctgacctcga
    16861 aagcgatgca ggcaagctcg cagatcagcg aaccggatta cggtgcactg ctggacgaca
    16921 tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag
    16981 tggagctggc ttttgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg
    17041 tttacaacgc cacggactat gtgatcccgg cgctggagct gatcgacgct cgctgccaca
    17101 acatcgatcc ggaaacccag cgcccgcgca aagtattcga caccatttcc gataacgccg
    17161 ccaacgccgg agtgatcctc ggaggtcgtc cgatcaaacc agacgaactg gatctgcgtt
    17221 ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg
    17281 tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg
    17341 tacaactgga aaccgggcaa atcattctcg gtggttcgtt caccctcccg gttccggcgc
    17401 gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg
    17461 tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgtccgcag
    17521 attggattat ggctggggct gagcagcagc tacagcgcgg agttactggc cggaccagga
    17581 ttcgactggt tattgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc
    17641 caactacagg cgattgcgcc ctaccccagc cagccagtgg tacgcccatc gtggaacgat
    17701 ccggtgcaaa tcaaacaact gctggacgta ggcacacaaa ccttactggt gccgatggta
    17761 caaaacgccg acgaagcccg taaagcggta cgcgccaccc gttatccccc cgccggtatt
    17821 cgcggtgtgg gcagtgcgct ggctcgcgcc tcgcgctgga atcgcattcc tgattacctg
    17881 caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag
    17941 aacttaccgc agattctgga cgtggaaggg gtcgacggcg tgtttatcgg cccggcggat
    18001 ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca ggccgccatt
    18061 gagcaggcga tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct gatcgccaat
    18121 gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac
    18181 accaccctgc tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtaa tagttcttct
    18241 atttcgatgg cgaagcaaaa caacaactct gtctactaaa cgtacgatgt ggagcgcatc
    18301 atgacgacct ctaccctgca atataaagat aataaaactg ttgaaattga acatcgtgtc
    18361 attaataaat tatttcgtcg cctaatcatt tttcttttta ttctgtttgt tttttcgttt
    18421 cttgatcgca tcaatatagg atttgccgga ctgacaatgg gcaaggatct tggcctgacg
    18481 tccacaatgt ttggcctcgc cgcaacactc ttttatgtca cgtacgtcat gtgcgggatc
    18541 cccagtaata tgatgttagc gaaaattggc gctcgtcgat ggatcgctgg gatcatggtg
    18601 gtctggggaa ttgcttccac ctgcacaatg tttgcaacaa gccccgaaac actctatcta
    18661 ctgcgtatgc tggttggtat tgctgaggcg ggttttttac ctggtatttt ggtttatctg
    18721 acctggtggt tcccggcata tcatcgggcc agagccaatg ccttatttat gatagcgatg
    18781 ccagtcacta tgatgctggg attgatcctt tcaggctaca ttctggcctt ggatggactg
    18841 tggaatctaa aaggctggca atggctcttt ttgctggaag gtctgccatc ggtcattctt
    18901 ggtgtcgtca cctggtttta tctcaacgac acaccagacc aggcgacctg gcttgatgaa
    18961 aacgaaaaac aggctttaaa aacgatgatt ctccgtgaac aagagagtgc atttgcccat
    19021 gcatctgttc cacgctcaac cttacgtgaa gtcctgaccc cagccatcat gctatataca
    19081 cttgcttatt tttgcccgac taatactctg agtgcgatta atatctggac gccgcaaatc
    19141 ctacagagtt tcaatactgg cagtagcaac atcatgattg gtttgctggc ggccatcccc
    19201 caattctgca caatttttgg catgatctgg tggagtcgtc gttccgaccg tcttaaagaa
    19261 cgcaaaaagc atacgattct tccctacctg tttgccgctg ccggatggtt actcgcttct
    19321 gcgacagatc acagcctcat tcaactgagc agcatcatta tggcctcaat gggatcattt
    19381 accgcgatga ctatcttttg gacaacaccg gatcaagtta taagcttgca atcgcgtgcc
    19441 gttgcgatgg ctgtcatcaa tgctgtcggc aatgtaggtt cagcagtcag tccattactt
    19501 atcggtattt tgaaagatgc aaccggcagt tttagctcag gtttgtggtt tgtagcagga
    19561 ttgctggtac tgggcgcgtt agtcatgacc cgaatcccta tggggagtcg ggaacactct
    19621 ctttcatctg aacttatcgc accaaaaccg caataaggag tcatgatgtc cagtgaagct
    19681 atctttgaac atggttatga aaatattgat ttggtccggg aatatgatgc ccgctacgct
    19741 ggtgaagatg tgcattatga aacatttgcc cggttagcga atttctttgg acgcgatatg
    19801 cgccctcact ggcacgatag atattttcaa ctccattatc tggtcactgg acgcattact
    19861 ttacaactgg atgagcactt ctattctgtg cgcgcaccgc tttttgtact aacaccacca
    19921 tcggtgccac atactttctt tactgatcct gatagcgatg ggcatgttct gaccgtacgt
    19981 caggagttaa tttggcccct ggtagaaaag ctctggcctg gtagtggaga tgccgcaatg
    20041 ctacaaggta tctgtctgtc actggaggga atggaacata cactggcggc attaaatcat
    20101 tattggccga taatatccct ggagtttcaa caaaataagc aaggacggga aattctattg
    20161 caatcactgg cgcaaagtat ttttacaatc ctactgcgtg aagcaccacc aaatgatatt
    20221 tcaacatgca gcgtgcgcgg cgagatgtgt ttattccaga agtttaatcg gctgattgac
    20281 gaaaattatc gccaacattt aatggtccct gaatatgcaa caatgctcgg tatgagtgaa
    20341 tcacggctta ccgagctgtg ccgacgtttt gccaatcaat cgccgaagag attgattttc
    20401 gaacgagtga gccgggaagc cagacggctt ctgctctata gcgagcaaag catcaaccag
    20461 atagcccttg atcttggcta caaagatccc gcctatttcg cccgtttttt caaccgaatg
    20521 atgggttgct caccaacaca attccgtggt cgctgaccat ttatcaacct gagagactcg
    20581 acatgaaaat atctttacgc tcactatttg tggcgtgcct gattgcgcca gtggcattca
    20641 cctacgcctc cgaaccgcaa taaatgaaaa ttgtactact tggaacgaaa ggtggccctt
    20701 ctttattaaa agctgaccgt ttaccgatgg caacggcact tattattggc aataaaacct
    20761 ggttaattga tgctggttac ggggccagct tacaactggt aaaaaataat attccactgc
    20821 gccagattga taatatttta ctcacccatt tacacagtga tcatattctg gactatccat
    20881 cagtcttaat gaatgcatgg gctagcggtc tgcaggacaa aactattcat gtattcggtc
    20941 cgccaggcac ccgagccatg accgctgcca gttggcaggt cttcgatcgg gacatcacac
    21001 tgcgtatgga agaagaagga aaacccgatc ctcgcaaact cgttacagct caagatgttg
    21061 gtgaaggcgt agtttataaa gatgataccg tgaccgtgac tgcattaaag gtccctcatt
    21121 ctcctttccc tgacggtgag gcttttgctt atcgcttcga cacacaaggc aagcgaattg
    21181 tcttctcagg agatacctcg tactttcccc cactggcaac tttctcagaa ggcgctgata
    21241 tacttgtgca tgaggccgta catgagccct ccgtcgctaa gctggcagac agtattggca
    21301 atggtaaaac actggcaaaa gccattgctt ctcaccatac gactattgag gatgtaggca
    21361 aaattgccaa tgaagcaaaa gtgaaaaaac tcatccttag ccacctggta ccaatcaccg
    21421 ttccggatga aacatggaag caagaagcac agaaaactat tcaggtccta tcgtcgttgg
    21481 tcatgaaaat atgacgatta ccattccatt cgccccataa aaaattccgt ccccgttagc
    21541 ccttgttagc ggggaatatt atgatttgcc ttactgtaag ttgatcacga tcgcagttta
    21601 ctatttaact ccaccttgct gtcgaaaagt accaacgttc gcctgaaacg tctcttcccg
    21661 acaaccgcgt tcaacgcttc aattaaataa caaaaacaac acacaaaatt aacaatcgta
    21721 ttccgattaa tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac
    21781 gtcctttcac cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct
    21841 atggcgagcg agtgaaagac gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg
    21901 ttgcccaact gtacgacgcg ctgcacaaac cggagatgca ggactctctg tgttggaaca
    21961 ccgacaccgg cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg
    22021 acctgcgcca gcaacgcgac gccatcgctg aatggtcacg cctgagctat ggctggatgg
    22081 gccgtacccc agactacaaa gccgctttcg gttgcgcact gggcgcgaat ccgggctttt
    22141 acggtcagtt cgagcagaac gcccgtaact ggtatacccg tattcaggaa actggcctct
    22201 actttaacca cgcgattgtt aacccaccga tcgtcatttg ccgaccgata aagtgaaaga
    22261 cgtttacatc aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt
    22321 ggttgccacc aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt
    22381 gatgggcgaa aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt
    22441 gaaattaatc tcccgcgcct cttatgagat ggtcgcgggt gctaccggct cgccatacga
    22501 ctacccgctc tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtact
    22561 gatcccatgg gaaaacgtgc tgatctaccg cgattttgat cgctgccgtc gctggacgat
    22621 ggaaggcggt ttcgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact
    22681 cgacttcatt acggcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg
    22741 tggtgtgcag gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag
    22801 tgactcgatg tgttcagaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc
    22861 cgcactgcaa acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat
    22921 agaacgcaac gttaccagtg gcctgatcta cctcccttcc agtgcccgtg acctgaacaa
    22981 tccgcagatc gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt
    23041 ccagcgcatc aagatcctca aactgatgtg ggacgccatt ggcagcgaat ttggtggtcg
    23101 tcacgagctg tatgaaatca actactccgg tagccaggat gagattcgcc tgcaatgtct
    23161 gcgccaggca caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct
    23221 gtcggaatac gaccagaacg gctggactgt gccgcacctg cacaacaacg acgatatcaa
    23281 catgctggat aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacaa
    23341 cgcctgcgct ttcatgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc
    23401 gagggcgacg ccggacaatg cgggattacg gcaacggctg tctgctcggt cacggataca
    23461 ccaccgtcgc tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc
    23521 aacggcaagt tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc gcgccacttc
    23581 gcgggcatga caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt
    23641 ccgctggcgc agccggtgct aaaaggttcg ctggccagtc ttgaaggaga gatccgcgat
    23701 gtgcaggcga ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt
    23761 gcagaaggtc acggacttat ttactttaaa cgccgtttcc atccggtgat gctggaaatg
    23821 gaagctgcga tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg
    23881 atctcaatca catatctacc cgatgaataa ttcttgccca accgctcacc gccattcctg
    23941 accgcttaca tccctaaaat aaccactcag ttatttacct tactttacgc gcacgtatct
    24001 ctggcaacat cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag
    24061 aacggcttcg gcctatgatt aacaaattac tctatggatt ttggtttgca ggaaggcagc
    24121 aagtgagtga atccccggga gcttacaaaa gtaagtgtca gaggtgaacg aacgcagctg
    24181 ccgcacctgt aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa
    24241 aactattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg
    24301 tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt
    24361 cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac
    24421 tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga
    24481 ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg
    24541 tgggaccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg
    24601 gggtagtgct ggccggtgcg gtacaggact ttatggtgct gtttatctcc tctcgccgta
    24661 acggcgcatc tctcggtgag atgatcaaag aagagatggg gccagtaccg gggactatcg
    24721 cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg
    24781 ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg
    24841 cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg
    24901 ttattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc
    24961 cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct
    25021 atgcgtttgt ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg
    25081 caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc
    25141 cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag
    25201 gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg
    25261 cgctgatctc ttccggtacg acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca
    25321 ttggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgcagcgt
    25381 ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgctggc cttggcatca
    25441 ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga
    25501 aagacgttac cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag
    25561 agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg
    25621 gtgcgccgac gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg
    25681 acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc
    25741 tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc
    25801 cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg
    25861 gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc
    25921 tgtggccgct gttcggtatc tccaaccaga tgctggcagc cgtagcgctg gtactgggca
    25981 ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg
    26041 tatggctgct tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc
    26101 agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg
    26161 acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg
    26221 caggcctgag tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa
    26281 cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa
    26341 tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc ccgcttcggc
    26401 ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat
    26461 ctcggccagg cggcgaagat gttgattggt attccagact acgacaacta cgtcgagcat
    26521 atgaagacca accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc
    26581 cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag
    26641 catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggca aaactaccct
    26701 gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt
    26761 cggcgaagtc tccgttgatg accaactgat tggcgaccgc gccacgcaga tcaaaacgct
    26821 gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaggacgcac tactcgacct
    26881 gctggataat ctcgacaagg gcaatattca gttcgatcgt ctggtgattg aatgcaccgg
    26941 catggccgat cccggcccga ttattcagac ctttttctcc catgagattt tatgccagct
    27001 ctacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa
    27061 ccagttcacc atcgcccagt cccaggtggg ctacgccgac cgtattctgc tgactaaaac
    27121 cgacgtcgca ggcgaagcag agaaactgcg tgaacgcctg gcgcgcatca acgcccgcgc
    27181 accggtctac accgtcaccc acggcgacat cgacctgggc ctgctgttca acaccaacgg
    27241 ttttatgctg gaagagaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggataa
    27301 acaaaacgat atttcgtcaa ttgtggtgga actggattac ccggtagata tcagcgaagt
    27361 ttcccgcgtg atggaaaacc tgctgctgga atcggcggat aaactgctgc gttacaaagg
    27421 gatgctgtgg attgacggcg aacctaaccg cctgctgttc cagggcgtcc agcgcctcta
    27481 cagcgccgac tgggacaggc cgtggggcga tgaaaaaccg catagcacaa tggtgtttat
    27541 cgggattcag ttgccggagg aagagattcg ggcggcgttt gcgggattga ggaagtaagt
    27601 tcgggaaaga tcaacttttt gagagttcca ccgcaacatt attacgagtg gaactcttta
    27661 gatatgtcag accaaccaaa gcttcttcaa ttgcgttttc attttttcat gtctcatttt
    27721 cagttcagcc aatccccatt ctgaagaatt acaaacatct tttgttaaat aaaaagagct
    27781 tttactatta agtttctcat aaatagattt ctttttgata aaatcatagt gttgtgtttc
    27841 agaatttttg gaaccactta tgagtgttaa attacctatt ttatgcagcc agtagacatg
    27901 ctcttgaggt tggaatctat taatccagta ttcattaact aatgcttgag gaagaatatg
    27961 ctcaattgtg attctccccg tataggtttt aatcacgctc tcatcttgtt gctccatatc
    28021 taagcgaagc aaaattgctt ttattaagtt cacttgattc ggacgaggct catataaatc
    28081 ttcatccaga gcagctataa aacctgaatt atctgcatgt tggtttatct gatttatcac
    28141 agaatcaaaa ggcatatcat tatttattgc taccagagca gaataacaaa ccatttctct
    28201 ttggctttta attcgttttt ttaaccaccc atgcatatat accttctcaa aggcggtaat
    28261 aaattgagaa aaatcatcta aattaaaatc ttctgtacga gccattcgat tcatgaatgc
    28321 cataaccgga ggaatccact catctacccc taaattactt agcgatgcta tttttcgtcg
    28381 tattgaaggg tgttcaaaat cattctctaa aa
//
LOCUS       DAJVQR010000050        28387 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVQR010000050 DAJVQR010000000
VERSION     DAJVQR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..123)
                     /gene="yjdI"
                     /locus_tag="OQM52_002267"
     CDS             complement(<1..123)
                     /gene="yjdI"
                     /locus_tag="OQM52_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCS1564098.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLF"
     gene            304..1935
                     /gene="dcuS"
                     /locus_tag="OQM52_002268"
     CDS             304..1935
                     /gene="dcuS"
                     /locus_tag="OQM52_002268"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1564099.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1932..2651
                     /gene="dcuR"
                     /locus_tag="OQM52_002269"
     CDS             1932..2651
                     /gene="dcuR"
                     /locus_tag="OQM52_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1564100.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3222..4562
                     /gene="dcuB"
                     /locus_tag="OQM52_002270"
     CDS             3222..4562
                     /gene="dcuB"
                     /locus_tag="OQM52_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1564101.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4640..6286
                     /gene="fumB"
                     /locus_tag="OQM52_002271"
     CDS             4640..6286
                     /gene="fumB"
                     /locus_tag="OQM52_002271"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1564102.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6409..7038
                     /locus_tag="OQM52_002272"
     CDS             6409..7038
                     /locus_tag="OQM52_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1564103.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7177..8598)
                     /gene="melB"
                     /locus_tag="OQM52_002273"
     CDS             complement(7177..8598)
                     /gene="melB"
                     /locus_tag="OQM52_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1564104.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8701..10056)
                     /gene="melA"
                     /locus_tag="OQM52_002274"
     CDS             complement(8701..10056)
                     /gene="melA"
                     /locus_tag="OQM52_002274"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1564105.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10339..11247
                     /gene="melR"
                     /locus_tag="OQM52_002275"
     CDS             10339..11247
                     /gene="melR"
                     /locus_tag="OQM52_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1564106.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11446..13713
                     /gene="adiA"
                     /locus_tag="OQM52_002276"
     CDS             11446..13713
                     /gene="adiA"
                     /locus_tag="OQM52_002276"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1564107.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14038..14799
                     /gene="adiY"
                     /locus_tag="OQM52_002277"
     CDS             14038..14799
                     /gene="adiY"
                     /locus_tag="OQM52_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1564108.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14936..16273
                     /gene="adiC"
                     /locus_tag="OQM52_002278"
     CDS             14936..16273
                     /gene="adiC"
                     /locus_tag="OQM52_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1564109.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16377..18020
                     /gene="eptA"
                     /locus_tag="OQM52_002279"
     CDS             16377..18020
                     /gene="eptA"
                     /locus_tag="OQM52_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1564110.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18017..18685
                     /gene="basR"
                     /locus_tag="OQM52_002280"
     CDS             18017..18685
                     /gene="basR"
                     /locus_tag="OQM52_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1564111.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18686..19786
                     /gene="pmrB"
                     /locus_tag="OQM52_002281"
     CDS             18686..19786
                     /gene="pmrB"
                     /locus_tag="OQM52_002281"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1564112.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19762..19851)
                     /gene="pmrR"
                     /locus_tag="OQM52_002282"
     CDS             complement(19762..19851)
                     /gene="pmrR"
                     /locus_tag="OQM52_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1564113.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19963..21465)
                     /gene="proP"
                     /locus_tag="OQM52_002283"
     CDS             complement(19963..21465)
                     /gene="proP"
                     /locus_tag="OQM52_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1564114.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21729..22607)
                     /locus_tag="OQM52_002284"
     CDS             complement(21729..22607)
                     /locus_tag="OQM52_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS1564115.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22604..24832)
                     /gene="crfC"
                     /locus_tag="OQM52_002285"
     CDS             complement(22604..24832)
                     /gene="crfC"
                     /locus_tag="OQM52_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1564116.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25235..25570
                     /locus_tag="OQM52_002286"
     CDS             25235..25570
                     /locus_tag="OQM52_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1564117.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25728..26171
                     /gene="yjdN"
                     /locus_tag="OQM52_002287"
     CDS             25728..26171
                     /gene="yjdN"
                     /locus_tag="OQM52_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1564118.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26304..27092
                     /gene="phnC"
                     /locus_tag="OQM52_002288"
     CDS             26304..27092
                     /gene="phnC"
                     /locus_tag="OQM52_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1564119.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27117..28133
                     /gene="phnD"
                     /locus_tag="OQM52_002289"
     CDS             27117..28133
                     /gene="phnD"
                     /locus_tag="OQM52_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1564120.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28239..>28387
                     /locus_tag="OQM52_002290"
     CDS             28239..>28387
                     /locus_tag="OQM52_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCS1564121.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7293 a         7415 c         6931 g         6748 t
ORIGIN      
        1 aaataacttg ccgttgccac gtacgcaatt gccagaatgc tgacatatcg cagtgttgaa
       61 atagacatcg atcttttcgc cggtataaca gcgataaccc ccgtccagta gcgcctgatc
      121 catgaccttc cctctctcat cagtatgatt atcgctataa gcatagcccc ggaatcttca
      181 tacagcactg gcagtttccg ttgccagagt ctcttcccct atatagccaa tcatttttac
      241 tccctttgaa ttacccgcct catcagagat aatgcttaag aaatttgtca cacaaggaag
      301 ctgatgagac attcattgcc ctaccacata ttacgcaaac gtccgatgaa attgagtacc
      361 actgtgatct taatggtcag tgcggtactg ttctcggtgc tattggtggt gcatctgatt
      421 tacttctcgc aaatcagtga tatgacgcga gacgggctgg ccaacaaggc actggcagtg
      481 gcgcgtaccc tcgccgactc gccggaaatc cgtcagggct tgcagaaaaa accgcaggag
      541 agtggcatcc aggccatcgc ggaagccgtg cgcaaacgca acgatctgct gtttattgtc
      601 gttaccgata tgcaaagtct tcgctactca catcctgaag cccagcgtat tggtcagcca
      661 tttaaaggtg atgacatcct taatgcgctg aatggcgaag aaaatgtcgc tatcaatcgc
      721 ggttttctgg cgcaggcttt acgcgtattt acccccatct acgatgaaaa tcataaacaa
      781 attggcgtgg tggcgatcgg ccttgagtta agccgcgtga cccaacagat caatgacagt
      841 cgctggagca ttatctggtc ggtattattt ggcatgctgg tcggactgat tggcacctgc
      901 attctggtta aggtactgaa aaaaatcctt ttcggcctgg aaccctacga aatctccacg
      961 ctgtttgagc aacgccaggc catgttgcag tctatcaaag aaggcgtcgt tgccgtggac
     1021 gatcgcggcg aggtcacgct gatcaacgat gccgcacaag aattgctgaa ttaccgtaag
     1081 tcgcaggacg atgagaaact gtcgacgctc agccactcat ggtcacaggt ggtagatgtc
     1141 tcggaagtgt tacgcgacgg tacgccgcgc cgcgacgaag agattacgat taaagaccgg
     1201 ctattactga tcaacaccgt tccggtgcgc agtaatggcg ttatcatcgg tgccatttca
     1261 accttcaggg acaaaactga agttcgtaaa ctgatgcagc gactcgacgg tctggtcaac
     1321 tatgctgacg cacttcgtga acgatcccac gaatttatga ataaattgca tgtgattctc
     1381 ggattattgc atctgaagag ttataagcag ttggaagatt acattctcaa aacagccaat
     1441 aactatcagg aagagattgg ctctctgctg ggtaagatca aatctccggt tatcgctggt
     1501 tttttaatca gcaagattaa ccgcgcgacc gatttaggcc atacgctgat tttaaacagt
     1561 gaaagccagc tgccagacag cggcagtgag gaccaggtcg cgacgctgat taccacattg
     1621 ggaaatctga tagaaaacgc gctggaagca ttagggccgg aacccggtgg cgaaattagc
     1681 gtaacattgc actaccgtca cggctggctg cactgcgaag ttaacgatga tggaccgggg
     1741 atcgcacccg acaaaatcga tcacattttt gacaaaggtg tctcgacaaa aggaagcgag
     1801 cgaggcgtcg gtttagcact tgtcaaacaa caggtagaaa atctcggcgg cagcatcgcc
     1861 gtggaatcgg aaccggggat tttcacacaa ttttttgtcc agataccctg ggacggggag
     1921 aggtcgaaca gatgatcaat gtattaatta tcgatgacga cgcaatggtc gcggagctga
     1981 atcgccgata cgtagcacaa atcccaggct ttcaatgctg tggaacagcc tcgacgctgg
     2041 agaaagccaa agagattatc ttcaatagcg atgcgcctat cgacctgata ttgctcgata
     2101 tctatatgca aaaagagaac gggctcgatt tactgcctgt cctgcataac gcgcgttgca
     2161 aaagtgatgt gattgtcatc tcctccgcag ccgatgcggc aaccattaaa gattcgctgc
     2221 attacggtgt cgtggattac ctgatcaaac ccttccaggc ttcccgcttt gaagaggcgc
     2281 tcaccggctg gcggcaaaag aaaatggcgc tggaaaaaca tcagtattat gaccaggccg
     2341 agcttgatca gctaatccac ggcagcagct ccaacgaaca ggatcctcgt cgcttgccaa
     2401 aaggcttaac gccgcagacg ttacgcacgc tgtgccagtg gattgacgcg catcaggact
     2461 atgagttttc aaccgacgag ctggctaacg aggttaacat ctcgcgtgtt tcctgccgaa
     2521 aatacctcat ctggctggtc aactgccaca tcttgttcac cagtatccat tatggcgtca
     2581 cggggcgtcc ggtttaccgt taccgtattc aggcagaaca ctactcactg ctgaaacaat
     2641 attgccaata acggatcgct ggttatctgt aagtaataat tattaccgta atgcaaaata
     2701 cccctggcac aagttacatc gttaatcagt aaaacttgtg ccagatcaaa tataattatc
     2761 cctccatcat cgttaaaaat taatatctct tcagatgaac ggtgttttta atttcaaaac
     2821 gataacaaaa gttaattaac tattatgtca cccgcattat gtgtattttt acccacaaat
     2881 gggtagatca gattaatcta taaacctaat gatatctgcc ctgagaacaa aaaatagacc
     2941 gataaatatc aataagataa cagcaaacaa aacattaaca tctgcgcagt acaaactata
     3001 aacccatcgc cagagagttt ttctctctga aaaagccgct tatcacagtg cataaatttg
     3061 ccgctgcttt aatcagccaa tattcactgt gaggtatttg ctaaagccgg taacgaccaa
     3121 acggatattt agtcaggctc tgaaaacagt tcatacaaaa cagaacgtga ctgtgatcta
     3181 ttcagcaaaa atttaaatag gattatcgcg agggttcaca catgttattt actatccaac
     3241 ttatcataat actaatatgt ctgttttatg gtgccagaaa gggtggtatc gcgctgggtt
     3301 tattaggcgg tatcggtctg gtcattctgg tcttcgtctt ccaccttcag ccaggtaaac
     3361 caccagttga tgtcatgctg gttatcattg cggtggtggc ggcatcggcg accttgcaag
     3421 cttcgggcgg tcttgatgtc atgctgcaaa ttgccgagaa gctgctgcgc cgcaacccga
     3481 aatatgtctc aattgtcgcg ccgtttgtga cctgtacact gaccattctt tgcggtacgg
     3541 gtcatgtggt ttacaccatt ctgccgatca tctacgacgt cgccattaag aacaacatcc
     3601 gtccggaacg tccgatggcg gcaagttcta tcggtgcaca gatggggatt atcgccagtc
     3661 cggtgtcggt tgcggtcgtg tctctggttg cgatgctggg taatgtcacc tttgatggtc
     3721 gccatcttga gttcctcgat ctgctggcaa tcaccattcc atcgacgtta atcggtatcc
     3781 tggcgatcgg tatcttcagc tggttccgcg gtaaagatct ggataaagac gaagagttcc
     3841 agaaattcat ctccgtaccg gaaaaccgtg agtatgttta cggtgatacc gcgacgctgc
     3901 tggataaaaa actgccgaaa agcaactggc tggcaatgtg gattttcctc ggggcaatcg
     3961 ctgtagtcgc ccttcttggt gctgattcgg acctgcgtcc atccttcggc ggcaaaccgc
     4021 tgtcgatggt actggttatt cagatgttta tgctgctgac cggggcgctg attattatcc
     4081 tgaccaaaac caatcccgcg tctatctcaa aaaacgaagt cttccgttcc ggtatgatcg
     4141 ccatcgtggc ggtgtacggt atcgcatgga tggcagaaac catgttcggt gcgcatatgt
     4201 ctgaaattca gggcgtactg ggtgaaatgg tgaaagagta tccgtgggcg tatgccattg
     4261 ttctgctgct ggtttccaag tttgtaaact ctcaggctgc ggcgctggcg gcgattgttc
     4321 cggtcgcgct ggcgatcggc gttgatccgg catacatcgt ggcttcagca ccggcttgct
     4381 acggttatta catcctgccg acttatccga gcgatctggc agcgattcag tttgaccgtt
     4441 ccggcaccac ccacatcggt cgcttcgtca tcaaccacag ttttattctg ccggggttga
     4501 ttggtgtgag cgtatcgtgc gtcttcggct ggatcttcgc cgcgatgtac gggttcttat
     4561 aaatgcactt tgcgtgccgc ccgtgactac gcggcacgcc attttcgaat aacaaataca
     4621 gagttacagg ctggaagcta tgtcaaacaa accctttatc taccaggcac ctttcccgat
     4681 ggggaaagac aataccgaat actatctact cacttccgat tacgttagcg ttgccgactt
     4741 cgacggcgaa accatcctga aagtggaacc agaagccctg accctgctgg cgcagcaagc
     4801 ctttcacgac gcttctttca tgctacgtcc ggcacaccag aaacaggttg cggctattct
     4861 tcacgatcca gaagccagcg aaaacgacaa gtacgtggca ctgcaattct taagaaactc
     4921 cgaaatcgcc gccaaaggcg tgctgccgac ctgccaggat accggcaccg cgatcatcgt
     4981 cggtaaaaaa ggccagcgcg tgtggaccgg tggcggtgat gaagaagcgc tgtcgaaagg
     5041 cgtctataac acctatatcg aagataacct gcgctactcg caaaacgcgg cgctggacat
     5101 gtacaaagag gtcaacaccg gcactaacct gcctgcgcaa atcgacctgt acgcggtaga
     5161 tggcgatgag tacaaattcc tttgcgttgc gaaaggcggc ggctctgcca acaaaacgta
     5221 tctctaccag gaaaccaaag ccctgctgac tcccggcaaa ctgaaaaact tcctcgtcga
     5281 gaaaatgcgt accctcggta ctgcagcctg cccgccgtac catatcgcgt ttgtgattgg
     5341 cggtacgtct gcggaaaaca acctgaaaac cgtcaaatta gcaagcgctc actattacga
     5401 tgaactgccg acggaaggga acgaacatgg tcaggcgttc cgcgatgtcc agctggaaca
     5461 ggaactgctg gaagaggccc agaaactcgg tcttggcgcg cagtttggcg gtaaatactt
     5521 cgcgcacgac attcgcgtta tccgtctgcc acgtcacggc gcatcctgcc cggtaggcat
     5581 gggcgtctcc tgctccgctg accgtaacat taaagcgaaa atcaaccgcg aaggtatctg
     5641 gatcgaaaaa ctggaacaca atctaggtca gtacattcca caagaactgc gtcaggccgg
     5701 tgaaggcgaa gcggtgaagg tcgaccttaa ccgcccgatg aaagagatcc tcgcccagct
     5761 ttcgcaatat ccggtttcca ctcgtttgtc gctcactggc accattattg tgggtcgaga
     5821 tattgcacac gccaagctga aagagctgat tgacgccggt aaagaacttc cgcagtacat
     5881 caaagatcac ccgatctact acgcgggtcc ggcgaaaacc cctgccggtt atccatcagg
     5941 ttcacttggc ccaaccaccg caggtcgtat ggactcctat gtggatctgc tgcaatccca
     6001 cggcggcagc atgatcatgc tggcgaaagg caaccgcagc cagcaggtta ccgacgcgtg
     6061 tcataaacac ggcggcttct acctcggtag catcggcggt ccggcggcgg tactggcgca
     6121 gcagagcatc aagcatctgg aatgcgtcgc ttatccggag ctgggtatgg aagctatctg
     6181 gaaaatcgaa gtagaagatt tcccggcgtt tatcctggtc gatgacaaag gtaacgactt
     6241 cttccagcaa atcgtcaaca aacagtgcgc gaactgcact aagtaacctc ttcggcccag
     6301 cgcctggcag catgctgcca ggtgatcccc ctgggccacc tcttttgcga ttgtaatttc
     6361 atgcttgctg gtgaatagtc agtattttcc ctgacttgcg aactcaccat gacccgcaca
     6421 ctcaagccgt taattcttaa cactggcgca ctggcgctaa cgttaatcct gatttatacc
     6481 ggcatttcgg cccatgacaa actcacctgg ctgctggaag tgacaccggt gattatcgtc
     6541 gtgccgctac tacttgccac cgccagacgt tacccgttaa cgctgctgct ctatacgctc
     6601 atcttctttc acgccatcat cctgatggta ggcgggcagt acacctacgc gaaagtcccc
     6661 gttggttttg aggtgcagga atggctgggg ttgagtcgta atccgtatga caagctgggg
     6721 cactttttcc aggggctggt gcctgcactg gtggcacgag aaattctcgt tcgcgggatg
     6781 tacgtgcgcg gacgtaaaat ggtggcgttt ctggtctgtt gcgtggcgct ggcgataagc
     6841 gcgatgtatg aattaatcga gtggtgggcg gcactggcga tgggccaggg agcggatgat
     6901 tttctcggta cccagggcga ccagtgggac acgcaatccg atatgttctg cgcgctgctt
     6961 ggcgcattaa cgacagtgat attgctcgct cgctttcatt gccgccagct acggcgctat
     7021 ggcttgataa ctggataaca acgtgcactt tgttgatgcc ggatgcggcg tgaattcctt
     7081 atccggtcta caaaatttgc aaatccaaag cattacatta agacgtggcc tgataaacat
     7141 atcgcatcag gccattttcg ttagctattg tccacgtcag gctttcacat cactcactgc
     7201 accgaccgga atatcagcat gaacaggctc gggcggtact ttgcgatatt tatccagcag
     7261 atgaatctgg atcctgcgca gcgtgtcgcc attgaggcga tagaaacgga agtagagaat
     7321 cagcgttacc atgaaaaaca gagtcggtag agcaatcata ataaactgca tacctaatag
     7381 ggcttgcgta gactgttcaa cattcggtac atagccaatc atccctaaca caaccgcaat
     7441 gaaaaaagcc gcaaaggctg aaccgccctt caccaccata gtctgcacgg agtaagcgat
     7501 gctttcacag cgtacgtgca gtttatattc accgtaatca acggtatctg ccaccatgat
     7561 gacctgtaat acccagaaaa gcgccgttcc cacattcagc aaaatacccg caatcacaat
     7621 gaggacgacg ttgtgatagc tcattaatgc cattaacagg agaacaccac agcttaacac
     7681 cggaagaata gatgctccgg cccataaaat gcgtcgggat aatgatttaa ctaagcgtgg
     7741 gaagaatact aacgtcacca ggttagcagc tcccgcatac gacagataat aggggaacaa
     7801 atccgcatca ccgataacat atgagaaata atagatagca aatccggtaa taatgttgct
     7861 ggctacatta taagcaagag ccatacccaa gaggcatgaa agctgatcgt ttttataaat
     7921 aagcgcaacg atggctttaa gtgtcagatg gcttccttca gcagacgatt gattgtctga
     7981 cgaaaagact tcatgcacat tgcgcagagt gatgatggtt gaaacaataa aaaaggcgat
     8041 caataccaga gtgaacatct gaaagccaaa tccccgatcg ccaccgccga cataattaac
     8101 aaatggtagc gtcacacctg ccgtaacaaa gcctgccaga ctggcaaaaa aacgcggata
     8161 aggaaccagt tgttcgcgtt cacgtttatc gagcgtgatg gttggaacca gcgaccagaa
     8221 gggaatatcc ataatggtgt aagtcatgcc ccagaggatg taggtcacgc aaacaaagac
     8281 aatctgagta gtaccttcaa acagatgcgc actaaagagg agaaataaga ttacagagtt
     8341 tgccaacgta ccgatcagga tccagggttt gaacttaccc catcgcgatc gcgtagcatt
     8401 tacaatccat cccataatcg gatcgttaat agcatcccag atcctcgcca ccagaaacaa
     8461 agtaccgacc aaacccacag acagcccgac gacatcggtg tagtaataca tgaggtacat
     8521 atacacaatg ccgatcgcaa aatccttccc gaacgctcca aatccataac tgagttttgt
     8581 agtcattgaa atgctcatag ggtatcgggt cgctgttacc aacgcccgcc ttctgttatt
     8641 ggtatcgtca gaggtgtgcg ccggaataaa tcccccggcg cagtagcgtt tagtcgcgtt
     8701 ttaacggtgc aaccagcttg gcagccagtc gccgtgggcg gcaatcaggt cgtcaacaag
     8761 agcatatatt tcgtcaatgc ccagcacggc ggcagtatgc gggtccatca tcgcggcgtg
     8821 gtaaacacgg tcgcgatttt ccgtaagaat agcttcggtc agcagcgtct gtacgttgat
     8881 gttggtttgc atcagggcgg ccagatgcga aggtagcgta ccgactttgg tcggctgaat
     8941 gccattagca tcaaccagac aggctacttc cacgcaacat ccttgtggca ggttatcaat
     9001 caaaccatcg ttacggacgt tgccgtaaat cacactcggc tcgccagtcc agatagcgtt
     9061 catgattgtg ctggcatatt cccgtgacgg tttaatatca atccgggagg cgtttttata
     9121 ctcctccagc tctttatgcc agttcgccag ctgctcgacg cagcgtttcg ggtactcatc
     9181 cagcggtact ttataacgct caatcaaatc ctcacgacct agcttaataa accacggcgt
     9241 atattcggca aaatgttctg atgattccgt gacgaagtag cccagctttt tgaacatttc
     9301 gtagcgcaca atattctggc agcgagtatt accatgaata tttggcttcg gtgcctgccc
     9361 tgcttcataa gccgccagca gttccgggta gagattcacg taactgccgt cggcggtttt
     9421 gcgctccagc tccaggtaaa acgccatatg gttgatacct gcgcaacggt aacgcagcgt
     9481 agccgggtcg atattgaggt cacgcgccag ctcttccgcc gttccctgca ccgaatggca
     9541 cagcccgacc tgtttgatat acggatagcg ggcatacatc gcccaggtat tcatcgccat
     9601 tgggttaaca tagttgagca tggtggcatc ggggcagact tccgtcatgt tctcgcaaat
     9661 ttgccacaga tgcggaatgg tacgtagcgc gcgcataata ccgcccggcc ccaacgtatc
     9721 ggcaatggtt tgttccagac catgccgctt acagacctcg aaatcagtca ccgtgcaagg
     9781 ttcataaccg ccaatctgaa atgccaccac gacaaaatcg gcatcctcta aggcttcttt
     9841 ctgttgggtg tggcaggtga ttttgccgct ggcccctgct gaatccatca gcttacgcac
     9901 cacaatatgc gactcttcca ggcgggtggg gtcaatgtcc atcagggcaa tatgcgccgt
     9961 tttcagcgcc tcgcgatgga acacatcacc aagaatattt ttaacgaaaa tcgtcgaacc
    10021 agcgccgata aatgtaattt tgggtgcaga catcatgcag atctcctggc ttgcttgaat
    10081 aacttcatca tggcaggcga atctgcagaa aacctccggc ttccaggttg agcattccca
    10141 taaactcaga tcctgcgtga agcagcagta aatctgagtt tatgggaata aattacctga
    10201 aaagtagagt tttcggcctc atgatgctcg gacagtcccc tcgcccctct gggaagaggg
    10261 tgaaaaccgt gctcccactc gcagtcatcc tccctcactc ctgccataat tctgatattc
    10321 taggaaagag agccatccat gaatacagat acgtttatgt gcagcagcga cgaaaaacag
    10381 acccgcagcc cgctgtcgct gtattcggaa taccagcgga tggagattga gtttcgcgca
    10441 ccgcatatca tgcccaccag ccactggcat ggtcaggtcg aagtgaatgt gcctttcgat
    10501 ggcgatgtgg aatacctgat caacaatgaa aaagtgaata tcaatcaggg tcatatcacg
    10561 ctgttctggg cctgtacacc gcaccaacta acagataccg gaacctgtca gagcatggcg
    10621 atttttaatc tgccgatgca tctgtttctc tcctggccgc tggataaaga cctgattaac
    10681 cacgtcactc acggcatggt gatcaaatca cttgccacac agcaacttag cccgtttgaa
    10741 gtacgccgct ggcagcagga attaaacagt ccgaatgagc aaattcgcca gctcgccatt
    10801 gatgaaattg gcctgatgct caagcgattt agcctctcta gctgggaacc gattctggtc
    10861 aataaaacct cgcgcacaca caaaaacagc gtctcgcgcc atgcgcaatt ttatgttagc
    10921 cagatgctgg gctttattgc cgaaaactat gatcaggcgc tgaccatcaa cgatgtggct
    10981 gagcacgtca aacttaacgc caactatgca atggggatat tccagcgggt catgcaattg
    11041 acgatgaaac agtacattac cgcgatgcgc atcaaccacg ttcgcgcgtt actgagcgat
    11101 accgataaaa gtattctcga tattgccctg acggcaggct ttcgttcaag tagtcgtttt
    11161 tacagcacgt tcggcaaata tgtcggcatg tcgccgcaac aataccgcaa acttagccaa
    11221 cagcgccgcc agacgtttcc cggctaaaga cgatatcagt atcagccaaa aaaatagttt
    11281 agccgtcgat aataagcagt gaaaataatt taaaataatc acataacgta ttgtttataa
    11341 aactaaatat ttcaaaaatg tttgtttttc acgcgcttta cagcccgaaa aggccggaag
    11401 atacttgccc gcaacgaaga ttccttcata accgggtaag caatgatgaa agtattaatt
    11461 gttgaaagcg agtttctcca tcaagacacc tgggtcggta acgccgttga gcgtctggca
    11521 gatgctttaa gccagcaaaa tgttaccgtg attaaatcca cctcctttga tgatggtttt
    11581 gccattctct cttcaaacga agccattgac tgcctgatgt tcagctatca aatggagcat
    11641 ccggacgaac atcaaaacgt cagacaattg atcggtaagc ttcatgagcg ccaacaaaac
    11701 gtgccggtct tcctgttggg cgatcgggaa aaagccctcg ccgcaatgga ccgggatctg
    11761 ctggagcttg tcgatgaatt cgcctggatt ctggaagata ccgccgactt tatcgccgga
    11821 cgcgccgttg ccgcgatgac ccgctaccgc cagcagctgt tgccgccact gttcagcgcg
    11881 ctgatgaaat atagtgacat ccatgaatat tcctgggcag cgccaggcca ccagggcggc
    11941 gttggtttta ccaaaacacc cgccggacgt ttctaccatg actactatgg tgaaaatctg
    12001 ttccgtaccg acatgggcat cgaacgaact tccctcggtt ctttgcttga ccatactggc
    12061 gcatttggcg aaagcgaaaa atatgccgca cgcgtatttg gtgccgatcg ctcctggtcg
    12121 gtagtcgtcg gtacttccgg ctctaaccgc accatcatgc aggcttgcat gaccgataac
    12181 gatgtcgtgg tcgttgaccg taactgccat aaatccatcg aacaaggttt gatgctgaca
    12241 ggcgcgaaac cggtctatat ggtgccaagc cgcaaccgct acggcattat cgggccaatc
    12301 tatccgcagg aaatgcaacc tgaaaccttg caaaagaaaa tcagtgaaag cccgctgacc
    12361 aaagacaaag ccgggcaaaa accgtcttac tgcgtggtaa ccaactgcac ctatgacggc
    12421 gtgtgttata acgctaaaga agcgcaggat ctgctggaaa aaacctccga tcgtctgcac
    12481 tttgacgaag cctggtacgg ctatgcacgt ttcaacccga tctatgccga tcactatgcc
    12541 atgcgcggcg aacctggcga tcacaacggt cctaccgttt tcgccaccca ctccatccac
    12601 aaactgctga atgcgctgtc acaggcttct tatattcatg tacgtgaagg tcgtggggcg
    12661 attaacttct cccgcttcaa ccaggcctac atgatgcatg ccaccacctc cccgctgtat
    12721 gccatctgcg catccaacga cgtggcggtg tcgatgatgg acggcaacag cggcctgtca
    12781 ctgacacagg aagtgattga cgaagcggtt gatttccgtc aggcgatggc gcggctatat
    12841 aaagagttca ccgctgacgg tagctggttc ttcaaaccgt ggaacaaaga agtcgtcacc
    12901 gacccacaaa ccggcaaaac ctatgacttt gctgacgcac caaccaaact gctgaccacc
    12961 gttcaggact gctgggtaat gcatccgggc gaaagctggc acggcttcaa agatattccg
    13021 gataactgga gtatgctcga cccaattaaa gtcagcatcc ttgctccggg aatgggtgaa
    13081 gatggtgaac tggaagaaac cggtgttccg gcggcgctgg tcactgcctg gcttggtcgc
    13141 cacggcattg tgcctacccg caccactgac ttccaaatta tgttcctgtt ctctatgggc
    13201 gtaacccgtg ggaaatgggg gactctggtt aacacccttt gctccttcaa acaccactat
    13261 gacgccaaca caccgctggc gcaggtgatg ccggaacttg ttgaacaata tcctgacact
    13321 tacgcgaaca tggggattca cgatctgggt gacaccatgt ttgcctggct gaaagaaaac
    13381 aaccctggcg cacggttgaa cgaagcctat tccggcctgc cggtggcgga aatcaccccg
    13441 cgtgaagcgt acaacgcgat tgtcgacaac aatgtcgaac tggtatccat tgaaaatctg
    13501 ccaggacgca tcgcggcaaa ctcagttatc ccgtatccgc caggaatccc gatgctgctg
    13561 tctggtgaaa acttcggcga taaaaacagt ccgcaagtaa gttatttacg ctcgctgcaa
    13621 tcctgggacc accatttccc tagatttgaa cacgaaactg aagggactga aattattgac
    13681 ggtatttacc acgttatgtg cgtgaaagcg taaccactat tccgctgaag gcgtaattgt
    13741 ttaaataaca ttacgccgcc tggccttagg ccttttgagt atggcaacgt tttcataaaa
    13801 attgctgcaa acaaaaatgt catacttttt gcgcggcccc accccacgct tttttttgcc
    13861 tgttatttat cctgtaaaaa aatatgtaca tgagaaatta ctataaaaat ttgtaatatt
    13921 agtaaaactc gttattttta tgcatgttta tattcatcat gcaattatat aaccatttcc
    13981 cggtatcgct ttgctttagc gagaactggt cttttattcg cactcaggag tacatgtatg
    14041 aggatttgca gcgaccaacc ttgtattgtt ttattgactg aaaaagatgt ctggataagg
    14101 gtgaatggga aagaacctat tagccttaaa gctaaccata tggcgttatt aaattgtgaa
    14161 aataatatta tcgacgtctc ctctcttaac aacactttgg ttgctcatat tagtcacgac
    14221 atcatcaaag attacctccg gtttctgaat aaagatctct cgcaaatacc agtatggcaa
    14281 cgtagcgcta cgcccatact caccctgcca tgcctgacgc cagacgtctt tcgcgttgcc
    14341 gcgcaacaca gcatgatgcc cgcagaaact gagtcagaaa aggaacgaac acgcgcatta
    14401 ttattcacgg tgctatcccg ttttctcgac agtaaaaaat tcctttcatt aatgatgtat
    14461 atgttacgta attgtgtaag tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa
    14521 gactggaatc ttagtatggt agccagttgt ttatgtctta gcccaagtct gttaaagaaa
    14581 aagttgaaaa gcgaaaacac cagttatagc caaataatca ccacctgccg catgcgttat
    14641 gccgtaaatg aattaatgat ggacggtaaa aatatctctc aggtatcaca gtcctgcggc
    14701 tacaacagta cgtcgtactt tatttctgtc tttaaagact tctacggtat gacgccgctg
    14761 cattatgtta gtcagcacag agaacgcact gtcgcctgat ttttaacctt aacgaagagc
    14821 tatattaata acggcatcag cgataacccg gtcgataata attcaactat cgaatgcagg
    14881 cgtatgatat gacgtaattt attgtcacga agctcgcctt cgcaggagtt taattatgtc
    14941 ttcggatgct gatgctcaca aagtgggctt aatccccgtc accctgatgg tgtcggggaa
    15001 tattatgggg tcaggtgttt ttctgttacc tgcaaacctg gcctccactg gcgggattgc
    15061 catttatgga tggttggtga cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa
    15121 aatgtcgttc ctcgacccaa gtcctggtgg ttcttacgct tacgcccgcc gctgctttgg
    15181 cccgtttctc ggttatcaaa ccaacgtcct ctactggctg gcctgctgga tcggcaatat
    15241 cgccatggtg gtcattggcg taggatattt aagttacttc ttcccgattc tgaaagatcc
    15301 attggtatta accatcacct gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt
    15361 cggtccgaaa atgatcaccc gcgtgcaggc cgttgccact gtgctggcgc tgatccccat
    15421 cgtcgggatt gccgtatttg gctggttctg gttccgtggt gaaacctata tggcggcatg
    15481 gaacgtcagc ggcctgggca ccttcggtgc gattcaaagt acccttaacg ttacgctgtg
    15541 gtcgttcatc ggtgtggaaa gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg
    15601 caatgtccct atcgccacca ttggtggggt attgattgcc gccgtttgct atgtgctctc
    15661 caccaccgcg attatgggga tgatccctaa tgccgcactg cgcgtttctg cctcgccatt
    15721 cggtgatgcc gcacggatgg cgttgggtga caccgctggg gccattgttt ccttctgcgc
    15781 agctgcgggt tgcttaggtt cactgggcgg ctggacgttg ctggcgggtc aaacggcgaa
    15841 agccgctgcc gatgacggac tgttcccacc gatttttgcc cgtgtaaata aagcgggtac
    15901 gccagtggcg gggctgatta tcgtcggtat tttgatgacc atcttccagc tcagcagcat
    15961 ttcaccaaac gcgaccaaag agttcggtct ggtttcttcc gtctcggtca tctttacact
    16021 ggtgccatat ctttacacct gtgcggcgtt actgctgctc ggccacggtc actttggtaa
    16081 agcacgcccg gcatatctgg ctgttactac cattgccttc ctctactgca tttgggccgt
    16141 ggtggggtcc ggggcgaaag aggttatgtg gtcatttgtc accctgatgg tcatcaccgc
    16201 catgtatgcg ctgaattaca accggctaca taaaaacccg tatcccttag atgcaccaat
    16261 aagcaaagat taattcccct taatccagca aacataaaag ccaaccttaa gaacttaagg
    16321 ttggcttaat tttgctttgc gagcatatgc gcactttgtt cgatggaaac accgtgatgt
    16381 tgaagcgcct actaaaaaga ccctctttga atttactcgc ctggctattg ttggccgctt
    16441 tttatatctc tatctgcctg aatattgcct tttttaaaca ggtgttgcag gcgctgccgc
    16501 tggattcgct gcataacgta ctggttttct tgtcgatgcc ggttgtcgct ttcagcgtga
    16561 ttaatattgt cctgacacta agctctttct tatggcttaa tcgaccactg gcctgcctgt
    16621 ttattctgat tggcgcggct gcacaatatt tcataatgac ttacggcatc gtcatcgacc
    16681 gctcgatgat tgccaatatt attgatacca ctccagcaga aagttatgcg ctgatgacac
    16741 cgcaaatgtt attaacgctg ggattcagcg gcgtgcttgc tgagctgatt gcctgctggc
    16801 taaaaatcaa acctgccacc tcgcgtctgc gcagtgttct tttccgtgga gccaatattc
    16861 tggtttctgt actgctgatt ttgctggtcg ccgcactgtt ttataaagac tacgcctcgt
    16921 tgttccgcaa taacaaagag ctggtgaaat ccttaagccc ctctaacagc attgttgcca
    16981 gctggtcatg gtactcccat cagcgactgg caaatctgcc gctggtgcga attggtgaag
    17041 acgcgcaccg caacccgtta atgcagaacg aaaaacgtaa aaatttgacc atcctgattg
    17101 tcggcgaaac ctcgcgggcg gagaacttct ccctcaacgg ctacccgcgt gaaactaacc
    17161 cgcggctggc gaaagataac gtggtctatt tccctaatac cgcatcttgc ggcacggcaa
    17221 cggcagtttc agtaccgtgc atgttctcgg atatgccgcg tgagcactac aaagaagagc
    17281 tggcacagca ccaggaaggc gtgctggata tcattcagcg agcgggcatc aacgtgctgt
    17341 ggaatgacaa cgatggcggc tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca
    17401 ccgcgctgaa tctgcctgat cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc
    17461 acgggctgga agagtacatc aataacctgc agggtgatgg cgtgattgtc ttacacacca
    17521 tcggcagcca cggtccgacc tattacaacc gctatccgcc gcagttcagg aaatttaccc
    17581 caacctgcga caccaatgag atccagacct gtaccaaaga gcaactggtg aacacttacg
    17641 acaacacgct ggtttacgtc gactatattg ttgataaagc gattaatctg cttaaagaac
    17701 atcaggataa atttaccacc agcctggttt atctttctga ccacggtgaa tcgttaggtg
    17761 aaaatggcat ctatctgcac ggtctgcctt atgccatcgc cccggatagc caaaaacagg
    17821 tgccaatgct gctgtggctg tcggaggatt atcaaaagcg gtatcaggtt gaccagaact
    17881 gcctgcaaaa acaggcgcaa acgcaacact attcacaaga caatttattc tccacgctat
    17941 tgggattaac tggcgttgag acgaagtatt accaggccgc ggatgatatt ctgcaaactt
    18001 gcaggagagt gagtaaatga aaattctgat tgttgaagac gatacgctgt tattgcaggg
    18061 actgattctg gcggcgcaaa ccgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat
    18121 ggcggaacaa agccttgagg acggtcatta cagcctggtg gtactggatt tagggttacc
    18181 cgacgaagat ggactgcatt ttctcgcccg tatccggcag aaaaaataca ccctgccggt
    18241 actgatcctc accgctcgcg atacgctgac cgacaaaatc gccgggctgg atgtcggtgc
    18301 cgatgactat ctggtgaagc cttttgcgct ggaagagtta cacgcccgta tccgcgccct
    18361 gctacgacgc cataataatc agggcgaaag tgagctgatt gttggcaatc tgacgctgaa
    18421 tatgggtcgc cgtcaggtat ggatgggcgg tgaagagttg attctgacgc ccaaagaata
    18481 tgctctgctg tcacggttaa tgctcaaagc aggcagtccg gtacatcggg aaattctcta
    18541 caacgacatc tataactggg acaatgaacc ctcgaccaac accctggaag tgcatatcca
    18601 caatctgcgc gacaaagtgg gcaaagcccg tatccgcacc gtgcgcggct ttggctatat
    18661 gctggtcgcg aatgaggaaa actaattgaa tctgatgcgt tttctgcgcc gacctatatc
    18721 gctgcgccaa cggctgatat tgaccatcgg ggccattttg ttggtgtttg agctgatcag
    18781 cgtcttctgg ctatggcatg aaagtaccga gcagattcag ctgtttgagc aggcgctacg
    18841 cgacaatcgc aacaacgatc gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct
    18901 gattgtcccc ggcgtcttta tggtcagcct gacgctattt atctgctatc aggcggtacg
    18961 ccgcatcacc cgcccgctgg cggagctgca aaaagagctg gaagcgcgca ccgccgacaa
    19021 cttaacgccc attgccattc acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt
    19081 gaacgatctg gtcagtcgcc tgaccagcac gctggataac gaaaggttgt ttaccgctga
    19141 cgtcgcgcac gaactgcgaa cgccactggc gggggtgcgt ttgcatctgg aactgctggc
    19201 gaaaacgcat cacattgatg tagcaccgtt agtggcacgg cttgatcaga tgatggagag
    19261 cgtctcccag ctgctgcaac tggcgcgtgc cggacagtca ttttcttccg gtaattatca
    19321 acatgtaaaa ctgctggaag atgtcattct cccctcgtat gacgaactca gtaccatgct
    19381 cgaccagcga cagcaaaccc tgctattgcc agagagcgcc gccgacatca ccgttcaggg
    19441 tgatgcaacc ctgctgcgga tgttattgcg aaacctggta gaaaacgccc atcgttacag
    19501 cccgcaaggc agcaacatta tgattaagct gcaagaagat ggcggagcgg tcatggcagt
    19561 tgaagatgaa ggaccaggta ttgatgagag taaatgcggg gagttgagta aagcgtttgt
    19621 acgtatggac agccgttatg gcgggattgg tctggggtta agtattgtca gccgcattac
    19681 acagttgcat cacgggcagt ttttcctgca aaaccggcaa gagacttccg gcacgcgggc
    19741 ctgggtacgg ctgaagaaag atcagaacgt ggcaaaccag atataaagaa agctgctgac
    19801 caccagcacg ctgaacacgg tagttaaact ttcataaaca cggtttttca ttgcactctc
    19861 ctccgttaac ctggaggaga gtatgcgcgt cagagattaa gtgaacctta agagttcaat
    19921 tggcctgata agacagcgtc acatcaggcc atccgtttca gcttattcat caattcgcgg
    19981 atgttgctgc accaggcggg tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatt
    20041 atcgattttc tgctcgatat tatcgtaatg ctcgacgaga atttccttcg cttcctgtat
    20101 atctgacgcc gccggtgtcg cacctttcaa cggacgattt gccgtctctt tcatggttac
    20161 gccggtgatt aaaccaatca ccgccactac catcaggtaa taggcaggca tcatcagatt
    20221 ctgcgagctt tcgaccagcc aggccgccag tgttggcgtc agaccggcaa ccagcaccga
    20281 aatattaaat gccgccgcca gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa
    20341 ggtagaagcc ataacgccca taaagcagtt aaggatcacc gccagcatca gtaacccggc
    20401 aaaaatcagg ccgatgacgt tactgttaat cagaataaac gccgggatcg ccaacacaaa
    20461 cagtgcaaca ctaccaagta gcacaaacgg acgacggcca aaacggtcac tcagcaagcc
    20521 catcaccggc tggacaaaca gcataccgat cataatggcg ataataatca gcaccccgtg
    20581 gtcttcggag taatgcaggt tatgcgacaa ataactcggc atataggtca gcaacatgta
    20641 gtaagtcacg ttggtggcaa ttaccagacc aatacatgtc aacaggctgc gccagtattt
    20701 agtggcaatc tctttaaacg agactttcgg gccatcctgc aaaccttcgc ggtcgccctg
    20761 ttccagttta tcaacatgct gctggaacgc cggagtttct tccagcgcat ggcgcaggta
    20821 aagcccgata atccctaacg gcagagcaat aaagaacgga atacgccagc cccagtcgag
    20881 gaagttcgct tcgccgacaa tggtcgaaat taacaccacc acgcccgcac ccagcacaaa
    20941 cccggcaata gaaccgaagt ccagccagct gcccataaag ccacgtttac ggtcagggga
    21001 gtattccgca acaaatatcg acgccccggt atattcacca ccgaccgaga aaccttgtgc
    21061 catcttacag atcaacagca gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat
    21121 taagccaata cagaacgtac tgatcgacat aatcacaata gtgatagcga ggatcttctg
    21181 gcgaccatat ttatcgccca acataccaaa gaagagtccg ccaagcggtc gaatcagaaa
    21241 gggaacggag aaagtggcaa gtgcagcaac catctgcacg ctggggtcag cccccgggaa
    21301 aaaaacttta cctaatgcgt aagcaacaaa accataaaca ccaaaatcaa accattccat
    21361 tgcattaccc agtgatgctg cggtaatggc tttacgcagt ttaccgtcat caataatggt
    21421 gacatcacga agggtaatcg gttttacttt tttccttttc agcatagctt tcctcgcaga
    21481 gttggcccat ttccgcaaac gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg
    21541 aattgcagga tgcaatctct gtaacaagcg tagcgggttt gcttacaccc tccggttaag
    21601 gaatgtacaa tcaaaccagt tgacgcctga attgggcagt taatgacctt tttaccctac
    21661 cagtgtttaa atttgtgatc aacttcacac atggatttcg tttaggactc attgatgtaa
    21721 ctgattattt acgccaaatt tacagagaag atttcctcga ccattgcccc caccaaacgc
    21781 tgcgctgaac agagtcgtgg catccccagc gcgacagttt gccagcgttg ggtcactgac
    21841 cagcaaacgg gttgtttatc tgccgtggtc cactttccca gccaacttaa aaaatcagca
    21901 tgatccccgc ccgtcggaac ggtcgaagag gcaaaccact gatggtaata gtgacgcgta
    21961 gtcggtgccg cattgatact ctctgccaga tgttgtgtcg ctttctcgcg caaatcaccc
    22021 agcaacaaca ccaccgcttg cgggcttccc agacggcagg catcgccaaa tgcaccccag
    22081 cgcaactcct ccagaacgat aaaacagcgc ccaggcaacg accaggcgtt atacgcccca
    22141 gcacgccagc gggtaaaaat ttgctcgctc tgatcacctg cctgcaccag taggcctcgt
    22201 tgcgttaacg ccttttcctt ccagcgacaa cgggctgaaa accactcata caaggcggca
    22261 atttgctggg ggagctccgg gaaagcagag tgctggcggt gatccatttt ttgcagcagc
    22321 gccagatgct gcgcgatctg cgtcagcgcc agatgaccgg gatccagcat cgacgccagt
    22381 ttttccacca ttgtcagtcg caggctaagc ggcgcattta acatgccgcc gttcgtccat
    22441 gtctgaagct gcacacgcga aagaagttcc tgcatcagtc gttcacgaaa ctgctgctga
    22501 tgggcttgag gatggcgggc atcatcgccc tgcgccagat ccactaaaaa gcgcggatga
    22561 accgactcca gcacgcgacc ggggccgtcc agtaacgtct tggtcaatat cgttctgccg
    22621 tgaaaagtgt ttgaatatca tcgcgtaaca gctgagcgtc ggtgtaaatc cagctgtgag
    22681 tcatcacagt ctgctgcaat tgctgctgca tcagcctgac caccgattca ttttgttgac
    22741 gcagagccag actttcgcgt aaacgcgtct gtaattctgt caaacatgaa gcgaactcag
    22801 cgaaaaaagt attcatgcct gccgtaacag attcatcgac ctgctctgcc agaactttag
    22861 ccatttgttg gcaatagaga tcgacttctg cgcttaatgc tcgttgcaac acactgtaat
    22921 caaccgtttc tgtcggggat ttctcatttc cccgtcccca gtcgggctga ttcaaccagc
    22981 gcgaaaaagt ctcacgcaca acgcctaaac gcgtgctctg ctcgtccgtt gcttcctggc
    23041 gcgaaatgac ttcactgaac agctggcgag tgttgaagtg gggaactacg ccgtgaaaaa
    23101 caggaaaatg aaacccagga cgaaaccctg actcgctcaa ttccattttg acttgttgct
    23161 caatggggcg aataacatcg gttaacaccc ggcaaagggt ggattccatc tcggcaaaac
    23221 gcagcgtaaa gtcgcgactg atggtgttct gcgccgtctg taacagtgtc tcacagcggg
    23281 tacgaatctc gcttaacggc tccgaatcat cctgaaacaa ggcggctaac tgcgcattca
    23341 ccgcatcttg ctgttgacgc agaaagtggt tggcggaggt cagggccagc tcgatttcat
    23401 gtttaatctc gccgctcacc tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga
    23461 cctgatggat attttgccgc aattgttcac aagcgacgtt taacccgtgc gcacgaaaat
    23521 ccaggtattc ccgcgcctgc tgcgcgtaat tcaacagttt atgcgcagca gatcgcagag
    23581 catacaatga ggcgttagcg taagcggcat gaagcaacgc ctgaattggc tgggcgaaca
    23641 gcgaatcttc ccacaactga tcggcagcat gacgaatatg ttcgaggtcc gccagatcgg
    23701 catgacgcca gcgcctgccg agcgcggcat gggcaaaatc ttccacccag cgttgttgct
    23761 ctggcgctgg taacttaccg ttgttggcta actcatagcg cgcccgattc gccaggtagc
    23821 cccacatcga cgacaccggg aatatctgct gtggcgtaat acagcctttc atcagcgtcc
    23881 cggaaatcag tgcccgcacc tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact
    23941 tattgaccag cacatacagc ggcaccgatt gccccaccgc caaaatcgcc tcacggacct
    24001 cttcatcgga gatcgatttc agttgcgtat aatccagcac cgccagtacc gccgaggcgc
    24061 gtgccagctg ctggttaagc attttttgca gatgcggttg cccggcttca tttggcccgg
    24121 gggtatccag taacgtcaac tgaccgggat aactttccag ccccgccaga tggacaaact
    24181 ccacttcaat cacgggaata tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt
    24241 ccagcgcctt cgccagtcgc actaaatcat tcaaactttt cagacaatga aaaataggct
    24301 gggcacccag ataatatttt tcgaaagcga cgccattttc gatccgctgc ataagcgcac
    24361 gcatatcttt atctatttcc agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc
    24421 gctgttgtaa ttgttgaatt aaacaatcga ttggcgcgac atgtgaaaaa tgcagtaccg
    24481 gttccttttg cccgggcgta tggcgaataa gcgtcggcag cgcagtcatt gggcgattac
    24541 gattaggcag aacctccgta ccaacaatgg cattaatggt ggttgatttc cctgctttca
    24601 tggtaccgac aattgccagc accatttcca gtcgggaaat tttacacaac tcattattca
    24661 gcatcgcgtg acgttcggcg atattaggct gactccaggg taaagccagt tgtggcgcgt
    24721 cgtctccggg tacagagaga ggcatttttt ccagtaactg caactgttgg cgagaaagct
    24781 gtaacaggcg ttcagcctcc tgacttaact catacagggt ctgtgtgtac atagaaaatt
    24841 cttccttaaa gcaaattttg ttacttttat ttagccagat tgtttttgag ttctgttttc
    24901 ggcttttata attactgcaa gaaataattt tatatttagt gtgttgtttt tttatcagaa
    24961 taaataacgt cttctgatgc gtttaaaacg tcagaaagat aaaaatatca tgtgaataaa
    25021 aaaaagaaca agtagagcat taacattatc ttaaataata aatagaggca aaaagattat
    25081 tttctttttg cgtttccttt caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc
    25141 gacaaactca gggattttgt gtataattgc ggcctttttc ggcaatctgc cgttttttgg
    25201 cgcttttgcc ctgctgactt ttgaggaaat ccacatgtca ttaccacact gcccaaaatg
    25261 caactccgaa tacacttacg aagataacgg catgtacatc tgcccggaat gtgcctacga
    25321 atggaacgac gcagaacctg cacaggaaag cgacgagctg atcgttaaag atgctaacgg
    25381 caatctgctg gctgacggcg acagcgttac catcattaaa gatctgaagg tgaaaggtag
    25441 ctcttcgatg ctgaaaattg gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca
    25501 taacatcgat tgcaaaatcg acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa
    25561 aaagaactga ttgtattgtg atcggtaagc cggataaggc gctcgcgccg catccggcaa
    25621 cgatgccgac tgcctgatgc gacgcttgcg cgtcttatca ggcagcctct catctgtata
    25681 aatttcgaac tacacttaac tggcttctct taactgaggt caccatcatg ccgttaagtc
    25741 cctacctctc ttttgccggt aactgttccg acgcgattgc ctattatcaa cgtacgttgg
    25801 gcgcggaact gctctataaa atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg
    25861 ccgagaactg cccttccgga atgcaatttc ccgataccgc catcgctcat gccaacgtgc
    25921 gcattgccgg aagcgacatc atgatgagcg atgctatacc gtcaggaaaa gccagctact
    25981 ccggctttac gctggtgctc gattcgcaac aggtcgaaga aggaaaacgc tggtttgata
    26041 accttgccgc taacggaaaa atcgaaatgg cctggcagga aactttctgg gcgcatggct
    26101 ttggcaaagt caccgataaa tttggcgtac cgtggatgat taatgtcgtc aaacaacaac
    26161 caacgcaata acccgccggg aggcccgccc tcccgcactg tcatcgaatt cccgttaact
    26221 cttcatctgt tagtcacttt taattaacca aatcgtcaca ataatccgcc acgatggagc
    26281 cactttttta gggaggctgc atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa
    26341 ccttcaatca gcatcaagcg ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg
    26401 tggctctgct tgggccgtcg ggttccggca aatccaccct tttacgtcat ttaagcggtt
    26461 tgattaccgg cgataaatcc gccggtagcc atatcgagct actgggccgc actgtccagc
    26521 gcgaaggccg cctggcccgc gatatccgca aaagccgcgc caacaccggc tacatctttc
    26581 aacaattcaa cctggtgaac cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg
    26641 gcagcacgcc gttctggcgc acctgtttta gctggttcac cggcgagcag aaacagcgcg
    26701 cgttacaggc gctgacccgc gttggcatgg tgcattttgc ccatcagcgc gtttccaccc
    26761 tctccggcgg ccagcagcaa cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag
    26821 tgattctggc cgatgaaccc atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg
    26881 acaccctgcg cgacatcaac cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg
    26941 tggattacgc cctgcgctac tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct
    27001 acgacggcag cagccaactg tttgataacg aacgttttga ccatctctac cgcagcatta
    27061 accgcatcga agagaacgcg aaagctgcct gatatcccca tcattgagga aaacgaatga
    27121 acgctaagat aattgcctcg ctggccttca ccagcatgtt cagcctcagc accctgttaa
    27181 ccccggcaca cgccgaagag caggaaaagg cgctgaattt cggcattatt tcaacggaat
    27241 cacagcaaaa cctgaaaccg caatggacgc cgtttttaca ggatatggag aagaaactgg
    27301 gcgtgaaggt caacgccttc tttgccccgg actacgcggg cattatccag gggatgcgct
    27361 tcaataaagt ggatattgcc tggtacggca acctgtcggc gatggaagcg gtggatcgcg
    27421 ccaatggcca ggtcttcgcc cagacggtcg cggcggatgg atcgccgggt tactggagcg
    27481 tgttgatcgt caacaaagac agcccgatca acaacctgaa cgatctgctg gcgaagcgga
    27541 aagatctcac cttcggcaat ggcgatccta actccacctc tggcttcctc gtccccggtt
    27601 actacgtctt cgctaaaaac aatatctccg ccagcgactt caagcgcacc gtcaacgccg
    27661 ggcatgaaac caacgcgctg gccgtcgcca acaagcaagt ggatgttgcc accaacaaca
    27721 ccgaaaacct cgacaagctg aaaacctccg cgccggagaa gcttaaagag ctgaaggtga
    27781 tctggaaatc gccgctgatc ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa
    27841 ctaccaaaga caagatctac gacttcttta tgaactacgg aaaaacgccg gaagagaaag
    27901 cggtgctgga acgcctgggc tgggcgccgt tccgcgcttc cagcgacctg caactggtgc
    27961 cgattcgcca gctcgcactg tttaaagaga tacaaagcgt gaaaggcaat aaagggctga
    28021 atgagcagga caagctggcg aaaaccaccg agattcaggc gcagctggat gacctggacc
    28081 gcctgaacaa cgcgttaagc gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag
    28141 gtcggataag acgccccggc gtcgcatccg acaatgtgta ggcgttgatg ccggatgcgg
    28201 tgcaagcacc ttatccggcc tacagaccgg agccaaacat gcaaaccatc accatcgccc
    28261 cacccaagcg tagctggttc tcgcttctga gctgggccgt tgttctcgac gtgctggtcg
    28321 tctcgtggca gggcgcggaa atggccccgc ttacgctgat taaagacggc ggcaacatgg
    28381 caacctt
//
LOCUS       DAJVQR010000051        27535 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVQR010000051 DAJVQR010000000
VERSION     DAJVQR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OQM52_002291"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OQM52_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1564122.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OQM52_002292"
     CDS             complement(1614..1937)
                     /locus_tag="OQM52_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1564123.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQM52_002293"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQM52_002293"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1564124.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQM52_002294"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQM52_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1564125.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQM52_002295"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQM52_002295"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1564126.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQM52_002296"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQM52_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1564127.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OQM52_002297"
     CDS             7537..8574
                     /locus_tag="OQM52_002297"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1564128.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQM52_002298"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQM52_002298"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1564129.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OQM52_002299"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OQM52_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1564130.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQM52_002300"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQM52_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1564131.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OQM52_002301"
     CDS             10745..11626
                     /locus_tag="OQM52_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1564132.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQM52_002302"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQM52_002302"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1564133.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OQM52_002303"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OQM52_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1564134.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQM52_002304"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQM52_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OQM52_002305"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OQM52_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1564135.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OQM52_002306"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OQM52_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1564136.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OQM52_002307"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OQM52_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1564137.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OQM52_002308"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OQM52_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1564138.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OQM52_002309"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OQM52_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1564139.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OQM52_002310"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OQM52_002310"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1564140.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OQM52_002311"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OQM52_002311"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1564141.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OQM52_002312"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OQM52_002312"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1564142.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OQM52_002313"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OQM52_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1564143.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OQM52_002314"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OQM52_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1564144.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQM52_002315"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQM52_002315"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1564145.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OQM52_002316"
     CDS             24893..25273
                     /locus_tag="OQM52_002316"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1564146.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OQM52_002317"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OQM52_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1564147.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQM52_002318"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQM52_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1564148.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27535
                     /locus_tag="OQM52_002319"
                     /pseudo
     CDS             26442..27535
                     /locus_tag="OQM52_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6789 a         6853 c         7223 g         6670 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgta
//
LOCUS       DAJVQR010000052        26903 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVQR010000052 DAJVQR010000000
VERSION     DAJVQR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..434)
                     /locus_tag="OQM52_002320"
     CDS             complement(<1..434)
                     /locus_tag="OQM52_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS1564149.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHP"
     gene            complement(421..1443)
                     /locus_tag="OQM52_002321"
     CDS             complement(421..1443)
                     /locus_tag="OQM52_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1564150.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(1457..2959)
                     /locus_tag="OQM52_002322"
     CDS             complement(1457..2959)
                     /locus_tag="OQM52_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1564151.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3099..4055)
                     /gene="ytfQ"
                     /locus_tag="OQM52_002323"
                     /pseudo
     CDS             complement(3099..4055)
                     /gene="ytfQ"
                     /locus_tag="OQM52_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4365..4895
                     /gene="ppa"
                     /locus_tag="OQM52_002324"
     CDS             4365..4895
                     /gene="ppa"
                     /locus_tag="OQM52_002324"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1564152.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5275..5616)
                     /locus_tag="OQM52_002325"
     CDS             complement(5275..5616)
                     /locus_tag="OQM52_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1564153.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(5619..9398)
                     /gene="tamB"
                     /locus_tag="OQM52_002326"
     CDS             complement(5619..9398)
                     /gene="tamB"
                     /locus_tag="OQM52_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1564154.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9395..11128)
                     /gene="tamA"
                     /locus_tag="OQM52_002327"
     CDS             complement(9395..11128)
                     /gene="tamA"
                     /locus_tag="OQM52_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1564155.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11334..11972
                     /gene="msrA"
                     /locus_tag="OQM52_002328"
     CDS             11334..11972
                     /gene="msrA"
                     /locus_tag="OQM52_002328"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1564156.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12295..13638
                     /locus_tag="OQM52_002329"
     CDS             12295..13638
                     /locus_tag="OQM52_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1564157.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13700..13906)
                     /locus_tag="OQM52_002330"
     CDS             complement(13700..13906)
                     /locus_tag="OQM52_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1564158.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14231..14785
                     /locus_tag="OQM52_002331"
     CDS             14231..14785
                     /locus_tag="OQM52_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1564159.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(14848..15786)
                     /locus_tag="OQM52_002332"
     CDS             complement(14848..15786)
                     /locus_tag="OQM52_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564160.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(15998..16738)
                     /gene="cysQ"
                     /locus_tag="OQM52_002333"
     CDS             complement(15998..16738)
                     /gene="cysQ"
                     /locus_tag="OQM52_002333"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1564161.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            16928..18871
                     /gene="cpdB"
                     /locus_tag="OQM52_002334"
     CDS             16928..18871
                     /gene="cpdB"
                     /locus_tag="OQM52_002334"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1564162.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(18997..19377)
                     /locus_tag="OQM52_002335"
     CDS             complement(18997..19377)
                     /locus_tag="OQM52_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1564163.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            19466..20326
                     /gene="qorB"
                     /locus_tag="OQM52_002336"
     CDS             19466..20326
                     /gene="qorB"
                     /locus_tag="OQM52_002336"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1564164.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            20435..21400
                     /locus_tag="OQM52_002337"
     CDS             20435..21400
                     /locus_tag="OQM52_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1564165.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            21508..22170
                     /gene="ytfE"
                     /locus_tag="OQM52_002338"
     CDS             21508..22170
                     /gene="ytfE"
                     /locus_tag="OQM52_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1564166.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22215..23627)
                     /gene="cycA"
                     /locus_tag="OQM52_002339"
                     /gene_synonym="dagA"
     CDS             complement(22215..23627)
                     /gene="cycA"
                     /locus_tag="OQM52_002339"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1564167.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(23935..24555)
                     /gene="fklB"
                     /locus_tag="OQM52_002340"
     CDS             complement(23935..24555)
                     /gene="fklB"
                     /locus_tag="OQM52_002340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1564168.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            24774..25319
                     /locus_tag="OQM52_002341"
     CDS             24774..25319
                     /locus_tag="OQM52_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCS1564169.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25303..25950)
                     /locus_tag="OQM52_002342"
     CDS             complement(25303..25950)
                     /locus_tag="OQM52_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1564170.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26162..>26903
                     /locus_tag="OQM52_002343"
     CDS             26162..>26903
                     /locus_tag="OQM52_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCS1564171.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYT"
BASE COUNT         6610 a         7141 c         6608 g         6544 t
ORIGIN      
        1 atgggatggt ttatcggaat cgactcttcc gaaacgagat agctgacgcc gcgcaaaaag
       61 aacatccccg caagcgtaat gataaatgcc gggatcttca gggcgtcgat cagaagcccc
      121 ataaatgcgc cgaaggcaca gcccatcacc agcactagcg gaaacgccag cagcggcgag
      181 aggccgaaat cgccaaccac ttttgccaga aacacgccag taaaggcgat caccgaaccg
      241 acggagagat cgataccgcc agagaggatc acaaaggtca tgccaacggc gattatccca
      301 agaaaggcgt tatcggtcag gatattgcag atcacccttg tggaagcaaa accgggaaac
      361 tgggtcaggc agtagagata gcccagcaca aagacgccga tggtgatcat cagcggcaag
      421 ttacgtttta tcatggctac gtactccttt aatcagactg ataaagcgtt gcgactgaac
      481 aatcagcaca caaagcacca ccaccgcttt caccacctgg ttcatctccg gcggaaagcc
      541 cgaaagcaaa attccggtgt tcatcccctg aataatcagc gcccccacta ccgagagcag
      601 caggttaaag cgcccgccca tcagcgatcc gccgccaatc accaccgcga gaatggcgtc
      661 cagctccagc cataatccgg cgttgttggc atcggcaccg cgaatatccg ccgccacgat
      721 aatgcccgca atcgccgcac atagcccgct caacacgtag gtgagcatga cgatgattcg
      781 cgtgtttacc ccggcatttt ttgccgcccg aatgttgata ccaacgactt cgataaacat
      841 ccccagcgcc gttttgcggg tcaacagcca gaacaggaga agcgtcagca ccgcgataat
      901 gaccggcgtt ggcaggaaca acagcgatcc gctgccaaac catgagagat ccggcgagtt
      961 aaacgtgacg atctgcccgg cggtgatcag ttgcgccacg ccgcgcccgg cgaccatcag
     1021 gatcagggtg gcgacaaacg gctgaatttt gaggatcgct accagtatgc cgttccacaa
     1081 tcccgccagg atgccggtgc ccagggcgct taacaaaaca atcggcaggc tgaatcccgc
     1141 gaccgtcatc gcagccgttg tggctccggc gatagccatc accgccccca cagagagatc
     1201 gataccaccg gtggcgatca ccagcgtaat accaatcgcc agtaacgcaa cgggagctgc
     1261 acggttaaga atgtcgatag ggctaccgaa caaacgccca tcctgaagca ccacctgcca
     1321 gaaatgcggg gctaccaggc tatcgaccag cagcaccagc aacagtgccg ccagctgcgg
     1381 catccccgtc ggccagtgaa aacgcctttt tggcggcgta gtgtcaggga gagattgagg
     1441 catcacactg ttctccttac gccgcaatgg cgttcatgat cgccggaacg gaaagctgtg
     1501 ccagcgggat ctccgccacc tgtttgcgat cgcgcatgat gatcacccgg tcggcatagc
     1561 ccaccagctc ttccagttcg gaggagatca ccagcagcgc cagaccgtca gcacacagcg
     1621 tttcaatcag gcggatgatc tcggcgtggg caccaacatc aatgccacgg gttagctcat
     1681 caaggatcag aaattgcggt cgggtcagta gccaacgtga aagcagcact ttttgctgat
     1741 tgccaccgga gagaaattca atcggttgtt cagttgaagg tgtgcgaatg ccaagctggc
     1801 ggataaagcg ttcggcaatc tcttgctgct ctttgcggga aatgggtcgt agccagccgc
     1861 gctgggcctg gagagcgagg atgatatttt cccgcaccga ggcggcggcg atgatgccat
     1921 cggttttcct gtcttccggg cagaatccga tgcccagtac tgaagcctga tgtggcgatc
     1981 gcagggtttg cggtttgcct ttgatcaacg ccgtgccgct gtcagcaggt ttgataccga
     2041 agatcacttc ggcggtttcg gtacgtccgg atcccagcaa gccagccaga ccgacgatct
     2101 cgccggggcg cacttcgaga tcaaacggtg ctatcgttcc ttttttgccg taatttttga
     2161 acgcggcaac gggtttgtcg ctcaacaatg ttcgcccggc acgctgtagc gcgtgggtat
     2221 ccagctcgcg ccccagcatc atttttacca gttcgatctg cggtagctcg cgcgtttccc
     2281 gacagcctac gaaactgccg ttgcgtaaga cggtgatccg atcgctgacc tgatagacct
     2341 gatcgagaaa gtgagtgaca aagatcaggc tgacgccgcg atcgcgcaac tgacgcatca
     2401 ggccaaataa tagctccatt tcctgggtgt cgaggctggc ggtgggttca tcgaggatca
     2461 gcactttggc ggagagatcg atagcccggc aaatggcgac gatttgctgc atcgccaccg
     2521 aaaaacggtt aagcggttcg cgcacgtcga gggagaaacc gtaagatgcc atcagttcgg
     2581 tggcgcgctt ttccatctct ttacggcgta gaaggccgaa acgtttgggt tcgcgaccta
     2641 tgaatagatt atcagcaacc gacatattgg gtagcaggtt gacttcctga tagacggtgc
     2701 cgataccgag ttgttgtgca tgggcggtat ttttcggtga gatagtctgg ccttccagcc
     2761 agatggtgcc gcgatcggcg tggtatacac cagttaatgc tttgattagc gttgattttc
     2821 ccgccccgtt ttcaccaagc agcgccatga tttcgccacg gcgcaggctg aaatcaacgt
     2881 tgtctaacgc tttgacgccg gggaaaaatt tacttaatcc ttcggtgcgg aggatctcct
     2941 ggtgttggtc ggtcgtcatg gtttcccccg gtgtaaagcg tgctcccagt aggcatgata
     3001 agacgcggta agcgtcgcat caggcttcag tgcaccatcg ccggatgcgg cgcgagcgcc
     3061 ttatccggcc tacgaatcgg catcaggcat agcaaccctc aataccccat atttttcttc
     3121 ttctctaact cttcttttgc ggtatcaggc aggtaaaggg tggatttggt taacgtcagc
     3181 ttttcaggca tggtgccgtc ttttttgtat ttctccagcg catcgaaagc gggtcccgcc
     3241 atgttcggcg tcagttcaac actggcgttc gcttcgccat ccatcatcgc tttgtagatg
     3301 tccggtacac cgtcaatgga acccgtgagg atatctttgc ccggtttcag gcccgcttct
     3361 ttaattgcct gaattgcacc aatcaccatg tcgtcgttat gggcgtaaac catgcagatg
     3421 tttttgccgt tgttttccgc tttgataaag ctctccatga cttctttgcc tttactgcgg
     3481 gtgaagtcac ctgactgcga gcggataatt ttgatatttg gcgcattctt aatggcttcg
     3541 gcaaagcctt tcttacggtc aatggcgacg ctggccccaa cggtgccctg cagctccacc
     3601 acgttgcatg gtttgccatt cacttctttt accagccagt caccgatcaa cttaccttcg
     3661 aggatgttgt cggcagtaac ggtggtcata tagagagatt tgtctttcac atcgatggaa
     3721 cggtcgagca agaagaccgg gatttcggca tctttcgcct cttttaatac cggttcccaa
     3781 cccgtcgcaa ccactggagc gataaagatc gcatccaccc cttatgcaac gaaggaacgt
     3841 accgctttaa tctggttttc ctgcttttgc tgaccatcgg caattttcaa cgtaattccg
     3901 cgtttttcgg cctcactttt cgccacattg gtttctgcgg cgcgccagcc ggattccgat
     3961 ccgacctgcg aaaatcctac ggttaatgga gcggccaacg ccatagacga catggctgcc
     4021 gagactgcag agactacaag taagcgtttc cacatagggt tgtcctcgtc ggggtaatta
     4081 ttattggtga aaagatgttc gcgaaaaaac tatagacaat tcgttatgta acggattgcg
     4141 ttacatcaca cttcaaaaaa gtaaataaaa cgttaatcac aagtttgtaa tcgctttcat
     4201 ctcactatga aaaatgcggc tacggttatg gattttcctg ctctgtatac cgtcttaaaa
     4261 ctggcgaaaa aggaaaatga agacgaaaac aagcaaagac attcggcgcg agttggctat
     4321 aatacttggc acttgtttgc cacatatttt taaaggaaac agacatgagc ttactcaacg
     4381 tccctgcggg taaagatctg ccggaagaca tctacgttgt tattgagatc ccggctaacg
     4441 cagatccgat caaatacgaa atcgacaaag agagcggcgc actgttcgtt gaccgcttca
     4501 tgtccaccgc gatgttctat ccgtgcaact acggttacat caaccacacc ctgtctctgg
     4561 acggtgaccc ggttgacgta ctggtcccga ctccgtaccc gctgcagcct ggttctgtga
     4621 tccgttgccg tccggttgcc gttctgaaaa tgaccgacga agccggtgaa gatgcgaaac
     4681 tggttgcggt tccgcacagc aagctgagca aagaatacga tcacattaaa gacgtaaacg
     4741 atctgcctga actgctgaaa gcgcaaatcg ctcacttctt cgagcactac aaagacctcg
     4801 aaaaaggcaa gtgggtgaaa gttgaaggtt gggaaaacgc agaagccgct aaagctgaaa
     4861 tcgttgcttc cttcgagcgc gcaaagaata aataagttct tctagcgcaa taaccctgaa
     4921 cgccgggctt cggttagtaa gggttttttt atgcccgcga taaataaact ctctattcca
     4981 ccatcattat tctcagcggt tgcaaggctt gaacggtaag aacaagcaaa cccgaccacc
     5041 attttgctgt tcatagccac ttgctggaag ttagccgacc tcactcatac tcaccgttca
     5101 aagcgtgccc ccgttttacc cctgcacatt tgtgaacgcc agcagagttt tttatgcgat
     5161 tgcagatatg gaaagcggct cggagagaaa agcaaaagat ggaaaagtta caaaaaaaaa
     5221 tcgcgagata cgtcgcataa aacaacgcca cccgaaggag gcgtgctttt attattactt
     5281 atccctgtct aaccagtcgc cgctttcaat tagctttaat ccatcgacgg gtcgttgata
     5341 aacgtacatc catgcactcc catacggcgt ctgaatcaac tggcgcgcgt attcaccgcc
     5401 cctggtgcgc aaggcatcaa gttcggccag cgtggcgttg tcaatacgat aaacttcacc
     5461 gtgtaccgtt ccgttccccg gaactgcgcc tggatagtgg cccaggctat acaactggta
     5521 gttatcgata ctgaaatcgc ccagtaactg ggcattggtc atccagtgac tgttgccttg
     5581 tttgtggcgt aaactgccgt agacaaatat tcgcattgct aaaactcgaa ctgatagagc
     5641 aaatccagtg cctggtctac accagacacg gcttccagat atagcttagg catcaggcga
     5701 taacgtaacg tgagtgttgc tatagagtca aatataccca cgccgtattt cacttgcaga
     5761 cctggcaata catagccgct gaccactacc tgggaggagt cgcctactcc cagggtgtcg
     5821 agcgctaaat tgcttacgcc aaacgtctcg ccgattttac ccacaatctg gccactttgc
     5881 gcaaccccca gaccaatcag catcgaggtc attgccgcac tgtcgctctg atcgctctcc
     5941 agcccttgtc cacgtagcaa ataagacaat gcagcttgtt gcgacatcgc cgggtcagag
     6001 aagatctccg ctttcggttc gtccgccaga ccagtgacgc gaactccggc gattacatcg
     6061 tcttctgtag catccgggtt acgaatagct tcaatattaa gatacggttg atccggcgga
     6121 ccagagaaca gtaactcacc cttacgcaca atcagatcct gaccataggc atggaagcgc
     6181 ccttcaggga tgttgatctg cccgttcagg cccagccctt gtttgtcctg tacgacgtta
     6241 agatcgcccg tcagccgcgc tttcaggcca aaggcgtcaa tgcgcacatt gttgccaacg
     6301 tggacaatca ggttactgtt aatcggaatc gacgccgttt tcggctcttc cggttgcagg
     6361 ttatcgttaa gcatcaccac atcgctggag acgcctactg cgctttccgg cagatcgtgc
     6421 accacgatgc gcgcccacgg gacatccacg cgaccatcga gggtaaacag gtttggtgta
     6481 gcctcgaata caacatctgg cgatacatcc attcgtacca tcggcggcac ggtgatccgc
     6541 actttactgc ctttcgccgt tactcgtgcc cgccagtttt caatttggct ccagtcggca
     6601 tcaccgttca ggtagatttc accctgctgg gtccgtactg taccggcaag cgtcgagcgc
     6661 ataccgttaa agttgaccgc aagctggctc ggctgcatat caaacggcat aaagttgccg
     6721 tcgatatcca cacccgtaac ctgaagctga ccaaacaact gcgggctttg cacatcacca
     6781 cccagacgca agttggcact caccatcccc gctgcttttt ccccacgggt aaagatgggg
     6841 tttatcatcg caaggttgaa gttacggata ttgacgttgc caccaagatt acggcggcct
     6901 tgcggatcgg tcacctgcac ctgtccatca aactggccgt tattggtcag gcggatggtc
     6961 cagcccaatt cggcacggtt gttacgcaat tccgccgtca gattcagtgt ctgaaacgcc
     7021 accggcagcg ccgcatcgtt gacggtttgc gttacctgca cgttacgccc cgaaagggtg
     7081 atactgccct gcggcagccc ctctttcgtg gtgtcccagg caacatccgc tttacccgtg
     7141 aagataccgc tggcctgagt ggtttctggc ataaacggtt tcagcatggc gaggtcgaag
     7201 cggttgagat tcaccaccgc acgcccttcg gcccccgcat cgatagtttg cggcacgcac
     7261 agttccgcat tcgggttaag ccagcagtgt ggcccgatgc tgattttttg ctccttattg
     7321 cggtaatcca gcgcaatatc gcgggtcagc gaccacgggc cgaccggcgt ctggaagcgg
     7381 gtattgctaa gagttccctt ccagcgttct tctttgcgat caaaacttcc tgccagatta
     7441 agctgcccgg agacaggctc gccctgaatc cgcaactgta gctcgtgctg cttttcgctg
     7501 cctttggcat tcagggtgac gaggttgata tttacatccg gctgcgaaat ttgctcaacg
     7561 cgtacgtcga gtttcccggc gatctgatcg gtggatttaa tgtcgccttc cacgcgaacc
     7621 tgcgccacgg aaagttcctg ccagcgcagg ccgcgcgcgg tgatatctgc cagtagttgt
     7681 ggcgcttcca ccgtgccgcg tactttcacc agccctttcg ctgtaccgcc aagccccggc
     7741 agcgcgttat ccagccccgg cgcgttgatg gtggcatcaa gattgagatc ttttaccccc
     7801 agctcgcctt tcacttcggc actgtttggc ccgagttcca gatgaagccc agggatcatc
     7861 cactgcatat aactgttgcc tttcagcgtg ccgtcaacgt tcactttgtt ctgtttgacg
     7921 ttaccggtca gcttcagttc tggcacctcc atctgccagg tgccgccgta caggctaccg
     7981 cgggttttaa tcaagccatt gagtttcgac ggccactccg ggaactcttt ggcggtgtta
     8041 atgccattaa gcgttagctc accgcgccaa ctaatggcct gctgccagtc gagcaacgcc
     8101 ttgagttcag ttttcccttc cagcgccgcg acggtgagtt tgtcgagatt gacctgctgt
     8161 tcattacctt tggcatcaag ggtaatggtg gctggcggga tctcctgtcc cttcactgcc
     8221 gtacgcatag agagcgtgta atcggtcatt ttgccggtaa gtttcagttt cagatcatcc
     8281 gcctgatact gcttctcacc agtgaacggc cagtaaagct gtttgctgtt cacttccacg
     8341 ttgagtggca atccggcttc cgccagtcgc gtctgggcgc gtaaatccat atccaccgga
     8401 ccggaaaggt taacgcctat ctccagctgt tcgcgcagcg cgccgcccac tttcagcttc
     8461 accttttcac ctttcaacgg ctccacgttc agtgtgctgt tgagggtgat atccaccggc
     8521 cagttgtctg acagctgcgc cgtgccgctg gcgttgacga tcccctggtt ggaatcgata
     8581 tccagggcgt ccagtttagt attgccgtca atgctgctca ctttcagcag catggtgcgc
     8641 acggtgatgt ccgtgtcgcc cgtcacgcgc agctgctcgc ccttaaactc ttcaatgttc
     8701 aggttaagcg gcaaatgcac gtcggtcatt tccggcaata ccgggcgaga aaagagatct
     8761 ttcagcgttt cgccgagcgg cttttcctcc ggctgcggat tttcaatttt cggttcgacc
     8821 acttcttcct gcgccacttc caccactttc ggcagagcaa tcagcaggcc tttcagcgac
     8881 gtcggtttca gggtcagggt tttctcctgc cagttcaggc cggaggtgaa gtccatcacc
     8941 gacaccgtgg tgtcatcaat cttgatgttg acgttgtcca gcgccacccg tgtcagggtg
     9001 atgggatacg gcgtggagag atccagcgga ccgctatctt cctcttcttc aacctgttca
     9061 gaaggaggca tttttttact gtcgatgttg acctgaatgt ctttcagcgc caggtcatta
     9121 atacaaacgc tactgttcca caggcactca agaccgaccc ccagatgcag attacccgct
     9181 tttaccgcca cgcctagctg ctcataacga acgtcagaca aggtgagatc gcgccagccg
     9241 ccggtgacct tgccaatatc cagtcctggc acccagcgat ccgccgcttt aaatacaaga
     9301 tgcaggccgc tggtggtgcc caccagaaac gccaccgatc ccagcaacag taagataacg
     9361 ataaccacgc cgaggctgat ttttttccat aaactcataa ttctggcccc agaccgatgt
     9421 aaaactgtaa cccgtgttcg tctttatccg cgaccggtac ggcaaaatcg agtttgattg
     9481 gcccgaccgg cgattcccag cgtacgccga ccccggtacc ggttttaaag tcgctgcggc
     9541 gaatatcgct taccgcttcg ccactatcga caaacaccgc gccccaccat tttccggtca
     9601 cgttgtactg gtattccagc gatccggtta tcaacttcga ggcccctttc aggtcaccgt
     9661 tggcgtattt cggggcgata gatttgtatt tgtagccgcg aatactgcga tcgcccccga
     9721 cgaagaaacg cagatccggc ggtactttgt cgaaatcacc ggtttcaatc cagcccagcg
     9781 tgccgcgtgt cacaaagcga tggcgatcgt acagtgtgcg gatccagacg tgctgcgcct
     9841 ggaaaacgga gaaatcgaca tctgaacccc aggccgtgtt ggagtagtcg atagagtagc
     9901 gttgcgagtc tccccaggtt ggcatcaggc caccacgaga acgcgtgcgg ctaatcatca
     9961 ccccaggata aaacagcatc gtggtgttgg taatttcacc ctgggtaaag tggtcgaggc
    10021 tccagcgcag gttaatggca cgctgccagc cgctggagag atcccagtag cgagaagcca
    10081 ccagcgtagt ggagtcagat tcggtatcgt tcaggtcagt gcgcttaaaa ccgccctgca
    10141 ccaaataata ttgttccagt ggattcttca gcagcggcat tttatagctg aagtcgaggg
    10201 tctgttccgg cgcggaaata ctggtactgg tggtcaaact gtgaccgtat gagttcatcc
    10261 acggcttttt ccacgtcgct ttcacgcgcg gtcccacgtc cgtagagtaa ccgaccccgg
    10321 tttcgatggt gttttctgtt cgcggcgaaa ccacgcccgt caatggtaat actttcgttt
    10381 cgcgcgcttt atcaaattgt ggagccacca ccaccgagtt aaaccagccg gtagcagaaa
    10441 gtcggcggtt cagttccgcc agatctttcg attcatactc atcgccctct ttaaacggca
    10501 ccagattttg caggtattca tcgcggattt gtgatccttc aaaggtcaca tgcccaaagc
    10561 ggtaacgttc gccactgtta taatcaatat cccagaaggc tttatgcagg ccgagcgcaa
    10621 tgcccagctg cgctttggta aattcgctat cgaaataacc tttacgcaac gcaatactgg
    10681 ttaaggactt tttgaaattt tcataatcgc cctggttcag taccgtacca atagccgggc
    10741 gagtatcgag caatttcaaa tagtctttat cggtccgcgc gccgccgcgc aataccacat
    10801 cggtgccgcc aattaacacc ggcacacctg gcgtgacttt ggcgatcaat acctgccgcc
    10861 ctttctttgg cggtggacgg agatcaaatt caatggtcgg ctggtaataa cccagcgctt
    10921 tcagaccttc acggatggca tcatcgacgc gtgcgcgaaa gcgacggtct ggcgtcactt
    10981 catcactttc aatcgtagaa agctgcgcac gaacgttctt ttccagctgt cccgataacc
    11041 cctcgacctg tagacggacg ttcgcggcga cggcagatcc gcttaagcag agtaagctta
    11101 cacagcataa ctgtcggata tagcgcacat tttctcctga atatcctttt ttcctgcccc
    11161 tggaacgccg ttaaacggct taacaaaaat ccagtaatat ggattaaaaa gcagactaaa
    11221 ccccaaatat ttcttatgtt ttactttaga cctattcacg gtggttattg tgtgcaaata
    11281 cgcctcttgt tacaacctta accccaatga ccgattttcg ggagagcgac accatgagtt
    11341 tatttgataa aaagcatctg gtttcccccg ccgatgccct gcctggacgt aacaccccga
    11401 tgcccgtagc cacgctgcat gcggtcaacg gtcactcaat gaccaatgta cctgacggaa
    11461 tggagattgc catttttgcg atgggttgtt tctggggtgt ggagcgtctg ttctggcagt
    11521 tacccggcgt ttacagcacc gccgcaggct ataccggcgg ctatacgcca aatccgactt
    11581 atcgggaagt gtgctccggt gatacgggtc atgccgaagc ggtacgcatt gtttacgatc
    11641 cttccgtcat cagctatgag cagttgctac aggtattttg ggagaatcac gatcccgccc
    11701 agggaatgcg tcagggcaat gaccacggca cgcagtatcg ttcagcgatt tatccgctga
    11761 ccccagaaca ggatgccgca gctcgcgcca gtctggaacg ttttcaggcg gcgatgcttg
    11821 ccgccgatga cgatcgtcac atcaccacgg aaatcgctaa cgccacaccg ttttattatg
    11881 ccgaagatga ccaccagcaa tatctgcata aaaacccata tggttattgt ggaattggcg
    11941 gaattggcgt ctgtctgccg ccggaagcat agcgttacgg gtacaaatgt agtttgttga
    12001 taaagtgctc tttgtctatg cctgatgcgg cttaaacgac ttaccaggcc tacaaaatcg
    12061 cccaaattca atatattgca aggactgcgt aggcctgatc ggcatagcgc atcaggcagt
    12121 attgcattta tcagcagtct gaattttaac cctctggcga ctttacagta ccttacgcta
    12181 tactagccac tgaaaatgcc ggttcacttt cttcgaatcg gctttcaatg tgtatttcac
    12241 acaaattaat caactttccc ttccgagggt ctggcctgaa aggtcggata agatatgtta
    12301 aacagtattt tagtcatact ctgcttgatc gctgtaagtg cgttcttctc gatgtccgag
    12361 atctcgcttg ccgcctcacg caaaatcaaa cttaaactgc tggctgatga aggcaatata
    12421 aatgcccaac gcgttctgaa tatgcaggaa aatcccggca tgttctttac cgtggtccaa
    12481 atcggtctga acgcagtggc gattctcggc ggtatcgtcg gtgatgcggc attttctcca
    12541 gcttttcaca gcctgttctc ccgctatatg tcggcagagc tctctgagca actgagcttt
    12601 attctctctt tctcgttagt gactggcatg tttatcctgt ttgcggattt aaccccgaaa
    12661 cgcatcggta tgattgcgcc agaagcggtg gctttgcgta tcatcaaccc gatgcgcttc
    12721 tgcctgtacg tttgcacccc gctggtgtgg ttcttcaacg gcctggcgaa catgatcttc
    12781 cgtattttca aactgccaat ggtacgtaaa gatgacatca cttctgatga catctacgcg
    12841 gtagtggaag ccggtgcgct ggcgggcgtg ttacgtaaac aggaacacga gctgattgaa
    12901 aacgtctttg agctggaatc ccgtaccgtt ccgtcttcaa tgacaccgcg tgaaaacgtg
    12961 atttggtttg atctccacga agatgagcaa agcctgaaga ataaggtggc ggaacatccg
    13021 cactctaagt tcctcgtctg taatgaagat attgaccaca tcatcggtta tgtcgattct
    13081 aaagacctgc tgaaccgcgt gctggctaac caaagcctgg cactgaacag cggcgtacaa
    13141 attcgcaaca cgctgattgt gccggatacg ttaacccttt cagaggcgtt ggaaagtttt
    13201 aaaaccgcag gtgaagactt cgcggtgatc atgaacgagt acgcgctggt ggtggggatc
    13261 atcaccctca acgacgtgat gaccacgctg atgggcgatc tggtcggtca ggggctggaa
    13321 gagcagattg tcgcccgtga tgagaactca tggctgattg acggcggcac cccaattgac
    13381 gacgtcatgc gcgtgctgga tattgacgag ttcccgcagt cgggcaacta cgaaaccatc
    13441 ggcggcttta tgatgtttat gctgcgtaag atcccgaaac gcaccgattc ggtgaaattc
    13501 gccggctaca aatttgaagt ggtggatatc gataactacc gcatcgacca gctgctggtg
    13561 acccggatcg acagcaaggc caccgccctt tcgccaaaac tgcctgacgc taaagataaa
    13621 gaagaaagcg tcgcgtaacc cccagaaaca tcaacggctc ctgaatcagg agccgtctta
    13681 ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc tgacggttaa
    13741 cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga atcttaccct
    13801 tatcaaattc aaaagcgcca atgtccttga tgtataaccg tccacggaac aggatcttta
    13861 cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt atctcctgct
    13921 gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc aaattttaat
    13981 gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt ttttacctaa
    14041 aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta taagcattca
    14101 tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg gaaattcgat
    14161 aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca tatcataaga
    14221 aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt tgccgatgat
    14281 ggcttccgca catcagttcg aaatcggtca gcgagtgccg ccgattggca ttaccgatcg
    14341 gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca gcgcgcagtt
    14401 agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa aagagaaaaa
    14461 cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt accagaccac
    14521 caccattgtt aacaccgacg acgcgattcc gggttccggc atgtttgtgc gcagcagtct
    14581 ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca atggcgtcgc
    14641 gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg ataaagacgg
    14701 tcgcgtgcaa tgggccaaag acggggcgct cacaccggaa gaggtgcagc aagtgatgga
    14761 cctgctgcaa aagttgctca aataatggca ataacataaa ttctggaaaa ataattttcc
    14821 cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacacc gaactaatac
    14881 tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gatttatatt tctcgcaaat
    14941 cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg catccacttg
    15001 ttcggctaat tcgacttgtt ggcaacctgc attaaccaga aaattataaa taagagagta
    15061 atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc ctccatagat
    15121 tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa ttattttttc
    15181 gtaaatatac catccatcgc attctttaat tccaccgcct ggtatttgga gttcaaattt
    15241 accccgtttt aatttgctta ctacatcctc caataaggca taaccgaaag atacgtcacc
    15301 aaacgcctgg cgaatataac ctttaatcct ttttggtatg tcagactgtt ttttctgcaa
    15361 aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt ttttgaaatc
    15421 catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa acccactgcc
    15481 tcgcaatgtt attgtatatt cagcaccgtt atctttaagg ctgaaactga taagaaaatt
    15541 atccatggca ttaacagttt cattttttga tgaaagcact tgataactat ttttatcaat
    15601 ctgtgttgcg caacgaaagg actcccgcaa agttaacaga ctatcataat tcgaaaccca
    15661 atcttttttc ttaaatgcat gctgaatatt attaaaaaaa tcgtttgaat gtgtttttgt
    15721 atgcgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc tgatcctggg
    15781 taacatatgg ctaccttatt ttatttggtt attttatgtc gccattaaat atctcattat
    15841 agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttactagca attgaatttt
    15901 tgcactttaa atacaatctc tttttattta taacttttta ggttgggata aatcaggatg
    15961 gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatggac actctgaacc
    16021 ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc cagtcgtgaa
    16081 cgtgcgctcc ggcagctgca gccacagcat gtccggcggc ggtgtcccaa atattcgttg
    16141 gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat ttcagcgaag
    16201 agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc agctccgcat
    16261 ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc tgaatcagct
    16321 tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac attacgttca
    16381 ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc gcaatgttaa
    16441 cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg tctaccagcc
    16501 agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca gaaaggaccg
    16561 gaatatccgg tgccagcgta cgtaaaccgt ccatgataac ggtgtgagcg gcaatatccg
    16621 ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc gtcccgtcgt
    16681 agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga tctaacattt
    16741 ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc tatcgttaat
    16801 gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg cattcttcac
    16861 tgttctataa gtaaggcgtt tattctccct tttctttcgt attcccgatg ataaaaggat
    16921 gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg ccagtgtgaa
    16981 tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca acatgatgga
    17041 tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta cggcaagcct
    17101 gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg gcgatttgat
    17161 tcaggggagt ccgctggccg attacatgtc ggcgaaagga ttaaaagcag gtgatgttca
    17221 tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg gcaatcatga
    17281 atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat tcccttatgt
    17341 aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt atttaattaa
    17401 agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg gctatattgg
    17461 cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggaa aagtgacggt
    17521 gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga aaggtgccga
    17581 tgtcattgtc gttctggcgc attccgggct gtctgccgat ccgtataaag taatggcgga
    17641 aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt ttggccatgc
    17701 tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata tcaccaaagg
    17761 cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc ttggggtggt
    17821 cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga aagcggaagc
    17881 acggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca gcaagctggt
    17941 agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc caatcggtaa
    18001 atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga ccgtgcaagt
    18061 cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc cggatctggc
    18121 aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca aaaatgaccc
    18181 ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg ccgatcttta
    18241 tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga aagagtggct
    18301 ggagtgctcc gccggacagt ttaaccagat tgctcctaac agcacgaaac cacagtcact
    18361 catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg tgaattatca
    18421 gattgatgtt acccaacccg cccgctatga cggcgagtgc cagatgatta atgccaatgc
    18481 agagcggatt aagaacctga cctttaatgg caagccgatt gatccgaacg ctatgttcct
    18541 ggttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg gcgacagcca
    18601 tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga ttgctgatga
    18661 gtcgaaacgt gcgggggaaa ttcatccggc ggcagataac aactggcgtt tagcaccgat
    18721 agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata aagccgcagc
    18781 gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg atatcgggtt
    18841 tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct ataaatcaac
    18901 cgcatccggc atttattggc ggatgcgatg ctgccgtatc ttatccaccc tacaagccat
    18961 gaaccgtaga ccggataagc gcagcgcatc cggcagttat gccgcacgtt catcccgcac
    19021 cgccaacacc tcgggcaaat tcaactcaat ccagtccgcc agtgcagcaa ccttttcgct
    19081 cacctgctcg cccagcggcg tgaggctata ttccacatgc ggcggcacca ccggatacgc
    19141 gatacggtta agaaaaccat cctgttctaa cgcctgtaac gactgcgcaa gcatcttttc
    19201 actcacccca ccgattttgc gccgcaggtc gctaaagcga tgagtacctt cgcgtagcgc
    19261 caccagaatc aacacccccc aacggctggt gacgtgtttc aacacctcgc gcgacgggca
    19321 ctgttccgca aagagattac cctctttcag ttgttgtgac aggctaacct gactcatttc
    19381 atacttacct ttttgcacgt acttactaaa agtaagttta ggtgttagcg tatttaaaca
    19441 caagacaaaa cgatggagac ttcccatgat cgctattact ggtgccactg gccaacttgg
    19501 tcactatatt attgaatcct tgatgaaaac ggttcctgcc agccaaatag tggctatcgt
    19561 tcgtaatccg gcaaaagccc aggccctggc agcacaaggc attaccgtgc gtcaggctga
    19621 ctacagcgat gaagccgcac tgacatctgc acttcaggga gtggaaaaac tactgctgat
    19681 ctcttccagc gaagtgggtc aacgtgcccc gcagcatcgt aatgttatta atgccgcaaa
    19741 ggcggctggc gtgaaattta tcgcttatac cagcctgcta catgcagata cctccccgct
    19801 cggcctcgcc gatgagcaca tcgagacgga gaaaatgctg gctgattctg gcatcgttta
    19861 caccctgctg cgcaacggct ggtacagcga aaactacctc gccagcgccc cggcagcact
    19921 ggaacacggc gtatttatcg gtgcggctgg cgatggcaaa atcgcctcag caacgcgggc
    19981 agattatgcg acagctgcgg cacgcgtgat tagcgaagcc ggtcacgaag gcaaggttta
    20041 cgaactggcg ggcgatagtg cctggacgtt gacacagtta gcggcagagc tgaccaaaca
    20101 gagcggcaaa caggttacct atcaaaatct gagcgaagcc gatttcgccg cggcactgaa
    20161 aagcgtcgga ctgcccgacg gactggcgga tatgctggcg gattctgacg ttggcgcatc
    20221 gaaaggtggt ctgtttgata acagcaaaac gcttagcaaa ttgattggct gcccaacgac
    20281 aacgttagcc gaaagcgtaa gccatctttt taatgttaat aactagttaa ttaaagtggc
    20341 atcctcccgc atcctctctg ataatgacgg gatgccggga gcaatcatgt ctgcttcctg
    20401 aacgtttctt ctgacagacc aatggatgcc agtaatgatt agcggcgtgc tgtacgccct
    20461 gttagcaggg ttgatgtggg ggcttatttt tgtcgggccg ttgatcgtgc cggaataccc
    20521 ggcgatgttg cagtcgatgg ggcgttatct ggcgttaggg ttaattgcgc tacccattgc
    20581 ctggctggga cgcgtgcgtc tgcgtcagtt ggcgcgtcgg gactggctta ccgccttgat
    20641 gctcactatg atgggcaacc tcatttatta cttctgcctt gccagtgcca ttcaacgtac
    20701 tggcgcgcct gtttccacga tgattatcgg caccctgccg gtggtgattc ctgtctttgc
    20761 caatctgctt tatagccagc gcgacggcaa actcgcgtgg ggaaaactcg ccccggcact
    20821 gatttgtatt ggcatcggcc tggcgagtgt gaatattgct gagttaaacc acggactccc
    20881 cgattttgac tgggcacgtt atacctctgg catcgtgcta gcgttagttt ccgtggtctg
    20941 ctgggcatgg tatgccctgc gcaacgcccg ctggctgcgg gaaaatcccg acaaacatcc
    21001 gatgatgtgg gcgacggcgc aggcgctggt cacgctgccg gtttctctca tcggctatct
    21061 cgtcgcctgt tactggctga atatacaaac gccggacttc tccttacctt ttggcccccg
    21121 tccgctggtg tttattagtc tgatggttgc gatagccgtg ctttgctcat gggttggcgc
    21181 actctgctgg aacgtcgcca gccagcgatt accgacagtg attctcgggc cgctgattgt
    21241 tttcgaaacg ctggcaggtt tgctgtacac ctttttactc cgccagcaaa tgccgccgct
    21301 aatgacgctg agcggtatcg cgctgttagt gattggcgtg gtcatcgcgg tcagagcaaa
    21361 accagaaaaa cctttaactg aatctgtctc agaaagttga catgctggca gtgagttaaa
    21421 taagcctctg ctacgtaagg gttatagctt ttgccttaaa ggtgtattta aaatacatct
    21481 tatcttatta agaatgaggt atcagctatg gcttatcgcg accaaccttt aggtgaactg
    21541 gcgctctcta tccctcgcgc ttcggctctg tttcgtaaat atgatatgga ttactgctgt
    21601 ggcggtaagc agacgctggc gcgcgcggcg gcacgtaaag aactggatgt tgaggtcatt
    21661 gaagctgaac tggcaaagct tgctgaacaa ccgattgaga aagactggcg tagcgccccg
    21721 ctggcagaaa tcattgacca tatcatcgtg cgctaccacg atcgtcaccg cgagcagctg
    21781 ccggagctga tcctgcaagc gactaaagtc gagcgcgttc acgccgacaa accgagcgtg
    21841 ccaaaagggc tgacaaaata cctgaccatg ctgcatgaag agctttccag ccacatgatg
    21901 aaagaagagc agatcctctt cccgatgatc aaacaaggca tgggcagcca ggcaatgggg
    21961 ccaatcagcg taatggaaag cgagcacgat gaagcgggcg aactgctgga agtgatcaaa
    22021 cacactacca ataacgtcac accgccgcca gaagcctgca ccacctggaa agcgatgtat
    22081 aacggcatta atgaactgat tgatgacctg atggatcaca tcagtctgga aaacaatgta
    22141 ctgttcccac gcgccctagc gggtgagtga tctaaaagct ggatggcatt gcgccatcca
    22201 gcatgataat gcggttattt ccgcagttca gcagcccgct tcttaccaat aaacagccag
    22261 cccaacccca gcgcgataaa ccacagcggg gtaaccagca gcgcctggcg agtgtcatct
    22321 tccagtgtca gcaacacgac cacgaacaca aagaacgcca tacataccca gcacatcagc
    22381 ttgccgagcg gcatcttgta gatcgacttt tcatgcagat gaggacgctg tttgcggtac
    22441 accaggtaag agcaaaggat aatcgtccag acgaacataa acagaatcgc ggaaacggtt
    22501 gtaatcatcg tgaacgcgcc aatcacgcta ggattcacat acaacatcac cacgccgccg
    22561 agcagacaga tacacgagaa ggtcaaccct ttcgcgggta ctgcgcgctt agaaagttta
    22621 gcgaacgctt tcggcgcaac accttcctgc gccagaccaa acagcatacg gctggtagag
    22681 aagacaccgc tgttagcgga agacgccgca gaggtcagca ccacaaagtt gatcacgctg
    22741 gcagccgcag gcaaacctac caacacaaac agctcaacga acgggctttt ctccgggact
    22801 accgaactcc acggtgtcac ggacataatc acaatcagcg cgaagacgta gaacataatg
    22861 atacggatcg gaatggagtt aatcgcgcgt ggcagtgatt tctccggatc tttggtttcc
    22921 gcagctgttg tacctaccag ctcaatcccc acgaaagcga aaaccgctat ctggaatccg
    22981 gcaaagaagc cacttaaacc tttcgggaac cagccgccgt cattccacaa atgcgcgaat
    23041 gacgcttcca caccggtcgg tgactgaaag tgcatcgcca ccatgaccag gccgacgaca
    23101 atcagcgaca cgatggcgac gattttgatc atcgcaaacc agaactccat ctcaccgaac
    23161 attttcacgg tggcaaggtt gagcgtcagc agcagcacta tcaccgccag cgaggcgacc
    23221 cagtcggaga gatcggggaa ccagaactga gcataagccg tgatcgccac cacgtctgcc
    23281 ataccggtta caacccagca gaaccagtaa gtccagccgg tgaaatatcc cgcccacggc
    23341 cccagtaaat cggaagcgaa gtcactaaaa gatttgtatt ccagattcga aagcagcaat
    23401 tcccccattg cccgcatcac gaaaaagagc ataaaaccaa tgatcatata aacgaaaatg
    23461 atcgacggcc cggcaaggct aatcgttttg ccggacccca taaacaaccc agtaccaatg
    23521 gcacctccaa tggcaataag ctgaacatgt cggtttgtga gattgcgccg tagcgactgt
    23581 tcagccggag cctgatcatc ggcaacgact tttacctgat ctaccatgtt ttttcttcct
    23641 gtacctgtct gtgttgttca ggctctacgg cctttagtca gtctatgata cgacgaatct
    23701 gtatcgggac tcatcgatat taggtaacaa tacgcgggat gaatactaag atttagatct
    23761 aatgttaatt ttatgtttaa agtgagtgtc atatcacggt gataacgcga aacagctcac
    23821 aaaaatacac gcttacgggg tctttgcaac acaaaatagc gccgtttggc aaactgaagg
    23881 gtttattgct gaatgcctgc tcccctctgg ttcgagggga acagaatgcg ctgcttagag
    23941 gatttccagc agttcgactt caaacaccag ggtgctgaac ggagggatgg atgcacctgc
    24001 gccgcgctcg ccatatgcca gttcctgcgg gatagtcagt tcccatttag aacctaccgg
    24061 catcagagtc agtgcttcaa tccagccagg gatcacgcca ttaaccggga attcagcggg
    24121 ttcaccacga gcaacggagc tgtcaaacac ggtgccgtcg atcagtttac cggtgtaatg
    24181 aacacgaacg cggtcggtac gtgccggaat tgcgccttca ccctggttga tcacgcggaa
    24241 ttgcaggcca gattcggtgc tattcacacc ttcttttttg gcgttttctt ccaggtattt
    24301 cacgccttca gcagccatcg cctggaaacg ctgacgacga acggcatcgg cgcgttcgtg
    24361 gatttcacgc agcgcgcgat gcaccacatc aaccggcaca gccggatgtt tgccttccag
    24421 cgcatcggca atacctgcaa ccagtgcttc tggcagcagc ccttccaggc cagattcact
    24481 cagttgttgc cctacctgca aaccaatgcc gtagcttgct tgcgcttcga tggtgtcaaa
    24541 agttggggtg gtcatcgtat ttcctttctt agggtataaa agtagcgggc agcatatcag
    24601 ccacacttct ttgggtaaaa ctttgtcgca ggaatgatga caaatcccgg ggaaaaagaa
    24661 acaatagcaa tatcccgtat gaatataata aaggcgggtt taccacggac atcatagcgt
    24721 tagcttattc ataggctatg attgaggaac aagacgtgga gcaggaggaa accatgcccg
    24781 ggcgctttga actaaaacca accctggaga aagtctggca cgcgccggac aattttcgct
    24841 tcatggaccc gctgccgccg atgcatcgtc gggggatcat tattgccgcc attgtattgg
    24901 tggtcggatt tctgcttcca tctgatgata cgcccaacgc gtcggtggtg acgcgtgaag
    24961 cgcagttaga tattcagtcg caatcacaac cgccgacaga agaacagccg caaactaccc
    25021 aaacgcaacc attccagcca gatagcggaa ttgataatca atggcgctct taccgtgtcg
    25081 aacccggtaa aacgatggcg cagctgttcc gcgaccacgg actgcccgct accgatgtct
    25141 atgcaatggc gcaggtagaa ggcgcgggta agccgctgag taatttgcaa aatgggcaga
    25201 tggtgaaaat ccgccagaac gccagcggcg tggtgacggg cttaaccatt gatacgggga
    25261 ataatcaaca ggtcctgttt acccgccagc cggatggcag ttttattcgt gcgcggtaga
    25321 tgaagggtca accaaaagcc agctaaggat tgtctctttg atgaccttac gtctggcttc
    25381 cagtgcctgg ggggaaacca tactgatatg ataaactagg ccaagcgtat gctggttgat
    25441 caaataatat cccccaagcg cagcaatatt aatattcacc tgcaatgggt cgatacctgg
    25501 cttaaaaata ttgtatttct tcccttcatc caataaggat tccattaact gcaagtgagc
    25561 atggttaatt tctaagagac gctgtgattt tgcataatgc acacctttac tttgattttc
    25621 actatgcaca atttttaaaa accaaggatt agcaatataa taatcccatg taaaatcaat
    25681 aattttctca atggcttttt gtggcggaag atctgagaaa tcaagggcct tttctttttg
    25741 tcgaatatcg ttccaggtat attccagcac ttcgataaat aaattttctt tattaccaaa
    25801 gtggtgataa accgtctgtt tactacaccc tgctgccttt acaatattat ctactctggc
    25861 cccttcataa ccatattcgg caaattcatt aaccgcacat agcagcaatt tttccttcag
    25921 gctctgtacg ttgctggtat caatatacat aacgcttccc cacgacctct tcgtggtgac
    25981 tatcataacc tgatttgatt tcagcttcag aaaaaagggc aaaaacttgt actttcatca
    26041 ggttatcaaa tgtcctgtga tcgccatcgc atatcaacca actagatggt ttatcttttt
    26101 atcctccagt cgcaagctaa tcatcaccaa ggaaacaaaa ttcaaaaaag agagctattg
    26161 tatgaaatcc actcgtcctg ttgccgtgat taccggagcc actcgcggta ttggaaaggg
    26221 ctgtgcgcag gagcttgctc gtggcgggtt taacctgctg attaacgatc gtcccgatgc
    26281 cgacagcgta gaaaaactgc atatcacaca acaagaatgt ttggcggaag gcgtggaagt
    26341 gatttgcttc cctgctgatg tcggtgatct atctctgcat gaggagatgc tcgacgcggc
    26401 gcaaaaccag tgggggcggc tggactgtct gctcaataac gcggggattt cagtgaaaaa
    26461 acgcggtgac ctcctcgacc tcgaaccaga cagcttcgat caaaacattg ccattaacac
    26521 ccgcgcgccg ttcttccttg ctcaggcatt cagtaagcgc ttactggcac agccgaaacc
    26581 ggaagcggag ctgccgcacc gctcgattat tttcgtcagt tccatcaacg ccatcatgct
    26641 ggcgatgaat cgcggcgaat acaccattgc caaaaccgcc gtttctgccg ctgctcgtct
    26701 gtttgctgct cgcctttgta atgaacaaat tggcgtttat gaagtgcgcc ctggtctcat
    26761 caaaactgat atgactattc cggcaacggc ttattacgac gaactgattg ccaaaggtct
    26821 ggtgccgtgg ggacgttggg gttatccggc tgatattgct tccaccgtcc gcgcgatggc
    26881 ggaaggtaaa ctgatttaca cct
//
LOCUS       DAJVQR010000053        25995 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVQR010000053 DAJVQR010000000
VERSION     DAJVQR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..346)
                     /locus_tag="OQM52_002344"
     CDS             complement(<1..346)
                     /locus_tag="OQM52_002344"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1564172.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVTTTVSKNH"
     gene            complement(479..784)
                     /locus_tag="OQM52_002345"
                     /pseudo
     CDS             complement(479..784)
                     /locus_tag="OQM52_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            852..1467
                     /gene="tnpA"
                     /locus_tag="OQM52_002346"
                     /pseudo
     CDS             852..1467
                     /gene="tnpA"
                     /locus_tag="OQM52_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1795..2877
                     /locus_tag="OQM52_002347"
     CDS             1795..2877
                     /locus_tag="OQM52_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCS1564173.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3366..4164)
                     /locus_tag="OQM52_002348"
                     /pseudo
     CDS             complement(3366..4164)
                     /locus_tag="OQM52_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4174..4725)
                     /gene="ybcL"
                     /locus_tag="OQM52_002349"
     CDS             complement(4174..4725)
                     /gene="ybcL"
                     /locus_tag="OQM52_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS1564174.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4894..5226)
                     /gene="emrE"
                     /locus_tag="OQM52_002350"
     CDS             complement(4894..5226)
                     /gene="emrE"
                     /locus_tag="OQM52_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS1564175.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5571..5885)
                     /gene="fliE"
                     /locus_tag="OQM52_002351"
     CDS             complement(5571..5885)
                     /gene="fliE"
                     /locus_tag="OQM52_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1564176.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            6099..7758
                     /gene="fliF"
                     /locus_tag="OQM52_002352"
                     /pseudo
     CDS             6099..7758
                     /gene="fliF"
                     /locus_tag="OQM52_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7751..8746
                     /gene="fliG"
                     /locus_tag="OQM52_002353"
     CDS             7751..8746
                     /gene="fliG"
                     /locus_tag="OQM52_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1564177.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEEHAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8739..9425
                     /gene="fliH"
                     /locus_tag="OQM52_002354"
     CDS             8739..9425
                     /gene="fliH"
                     /locus_tag="OQM52_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1564178.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9425..10798
                     /gene="fliI"
                     /locus_tag="OQM52_002355"
     CDS             9425..10798
                     /gene="fliI"
                     /locus_tag="OQM52_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS1564179.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10820..11161
                     /gene="fliJ"
                     /locus_tag="OQM52_002356"
                     /pseudo
     CDS             10820..11161
                     /gene="fliJ"
                     /locus_tag="OQM52_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11158..12285
                     /gene="fliK"
                     /locus_tag="OQM52_002357"
     CDS             11158..12285
                     /gene="fliK"
                     /locus_tag="OQM52_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1564180.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12390..12854
                     /gene="fliL"
                     /locus_tag="OQM52_002358"
     CDS             12390..12854
                     /gene="fliL"
                     /locus_tag="OQM52_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1564181.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12859..13863
                     /gene="fliM"
                     /locus_tag="OQM52_002359"
     CDS             12859..13863
                     /gene="fliM"
                     /locus_tag="OQM52_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1564182.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13860..14273
                     /gene="fliN"
                     /locus_tag="OQM52_002360"
     CDS             13860..14273
                     /gene="fliN"
                     /locus_tag="OQM52_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1564183.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14276..14641
                     /gene="fliO"
                     /locus_tag="OQM52_002361"
     CDS             14276..14641
                     /gene="fliO"
                     /locus_tag="OQM52_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1564184.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14641..15406
                     /gene="fliP"
                     /locus_tag="OQM52_002362"
                     /pseudo
     CDS             14641..15406
                     /gene="fliP"
                     /locus_tag="OQM52_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            15416..15685
                     /gene="fliQ"
                     /locus_tag="OQM52_002363"
     CDS             15416..15685
                     /gene="fliQ"
                     /locus_tag="OQM52_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1564185.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15694..16479
                     /gene="fliR"
                     /locus_tag="OQM52_002364"
     CDS             15694..16479
                     /gene="fliR"
                     /locus_tag="OQM52_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1564186.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16764..17387
                     /gene="rcsA"
                     /locus_tag="OQM52_002365"
     CDS             16764..17387
                     /gene="rcsA"
                     /locus_tag="OQM52_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1564187.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17431..17619)
                     /gene="dsrB"
                     /locus_tag="OQM52_002366"
     CDS             complement(17431..17619)
                     /gene="dsrB"
                     /locus_tag="OQM52_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1564188.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17782..18009
                     /gene="yodD"
                     /locus_tag="OQM52_002367"
     CDS             17782..18009
                     /gene="yodD"
                     /locus_tag="OQM52_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS1564189.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18307..19122
                     /locus_tag="OQM52_002368"
                     /pseudo
     CDS             18307..19122
                     /locus_tag="OQM52_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(19119..20813)
                     /gene="yedQ"
                     /locus_tag="OQM52_002369"
                     /pseudo
     CDS             complement(19119..20813)
                     /gene="yedQ"
                     /locus_tag="OQM52_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20984..21166)
                     /locus_tag="OQM52_002370"
     CDS             complement(20984..21166)
                     /locus_tag="OQM52_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1564190.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21245..22162)
                     /locus_tag="OQM52_002371"
     CDS             complement(21245..22162)
                     /locus_tag="OQM52_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1564191.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22335..23255
                     /gene="yedA"
                     /locus_tag="OQM52_002372"
     CDS             22335..23255
                     /gene="yedA"
                     /locus_tag="OQM52_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1564192.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23244..23714)
                     /gene="vsr"
                     /locus_tag="OQM52_002373"
     CDS             complement(23244..23714)
                     /gene="vsr"
                     /locus_tag="OQM52_002373"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1564193.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23695..25113)
                     /gene="dcm"
                     /locus_tag="OQM52_002374"
     CDS             complement(23695..25113)
                     /gene="dcm"
                     /locus_tag="OQM52_002374"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1564194.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25180..25720)
                     /locus_tag="OQM52_002375"
                     /pseudo
     CDS             complement(25180..25720)
                     /locus_tag="OQM52_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25760..>25995)
                     /locus_tag="OQM52_002376"
     CDS             complement(25760..>25995)
                     /locus_tag="OQM52_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564195.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         6539 a         6659 c         6408 g         6389 t
ORIGIN      
        1 cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga
       61 tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga
      121 ccttgcggct taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt
      181 ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca
      241 gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac
      301 tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct
      361 gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg ctgttttcct
      421 gagcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc
      481 aggggaacgc cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg
      541 actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg gcatttttcc
      601 actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg gccttatgat
      661 tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc
      721 tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt
      781 tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc ggaaggcact
      841 gtgttttcct gatgcatgct caccgggtct ttgttaccaa gtaccgacgc cagatttttg
      901 accatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag
      961 ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tctgttaatt aactatcccc
     1021 ccaaactggc gatatccagt ctggtaaaca aacagcctca aaggcgtatc gggtaggtta
     1081 ctgcgacggg atcgaccaga tattgctgcc aggtattacc gcaaagatgt tttgtggcgt
     1141 cccggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg ccaatacatt
     1201 gaacaacagc aaacacctgg tcagatgaaa aaacgagcct tatatccccg ccctgaagga
     1261 cgggctttta cggtgcactg gataagcctg gagattatct acacctgcat ggacatcatt
     1321 gtactatgcc ccgtacatgc tccatcacat cctcaacatc aaagtattag agccttgcaa
     1381 accattatgc gtctcagttt gtctcatcta tcttacgcaa aaaaaataga actaatatca
     1441 agcggcgcta tattgcgtat taaataaaac cacctcaatt acattaataa atctaaataa
     1501 atatcatgca aataatttcc atggttattt ttaggctatt taaattaaaa aaataaatta
     1561 tgagtcaatc ataaataaca acctttaata taatttgact accaaattta tattcgttca
     1621 caaaatagtc atgcattgtg tgcaatagaa acagttcaga taaaaatagg gatctacttc
     1681 acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat tggatgaata
     1741 tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct cacgatgaaa
     1801 aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat gtctgctcag
     1861 gcagcagaga tttacaataa agacagtaac aagctggatc tgtacggaaa agttaatgct
     1921 aagcactact tctcttctaa cgatgcagat gatggcgaca caacttatgt tcgtctgggc
     1981 ttcaaaggcg aaactcaaat caacgatcaa ctgactggtt tcggtcagtg ggaatatgaa
     2041 ttcaaaggca accgtgctga atctcaaggt tcttccaaag ataaaacccg tcttgccttc
     2101 gctggcctga aattcggtga ctacggctcc atcgattatg gccgtaacta cggtgtagca
     2161 tacgatatcg gtgcatggac cgacgttctg ccagaattcg gtggcgatac ctggacccaa
     2221 acagatgtgt tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa caacgacttc
     2281 tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa tgaccgcact
     2341 gacgtaactg aagctaacgg tgatggtttc ggtttctcca ctacttatga gtatgaagga
     2401 ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta atgatcaggt tatctacggt
     2461 aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg tctgaaatat
     2521 gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat gactgttttt
     2581 ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca atatcagttc
     2641 gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga cttgggtgcg
     2701 tggggtgatc aggacctgat tgaatatatt gatgtaggtg caacctatta cttcaacaaa
     2761 aatatgtcca cttttgttga ttacaaaatc aacctgattg ataagagcga tttcacgaaa
     2821 gcatctggcg ttgctaccga tgatatcgtt gctgtaggat tggtttacca gttctaatct
     2881 gattactcaa agatatgttg tgggaggctt tgcctcccca acatataagt ggcgctctca
     2941 agccactttc cttagaagca ctatcttgct tctgactata taaattaccc tttattttct
     3001 gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt atcaattaga
     3061 tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca tccctttaat
     3121 tatcccctca acctcttgta aatttcccac tcttgagctc cacatagccc aaacgccgga
     3181 tgcaaaaaaa atcatacgtg catctctgaa attgtgatgg aggaagtgag tatcaattat
     3241 atgtgcttat aaacgttccc gattggcaca gaagtcaccg gattgttcag gctccaatag
     3301 gttgagtatg tcgtatttat tttccaaaat aaaaatcgat gctgttgctc cttaataaaa
     3361 tacttttact ctctggacgc ttttcttagc tctcttggtg ttatattaaa acgattatgg
     3421 aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat ctcgctgatg
     3481 ggttttactg tcgtttgtat agcaggacaa cgcattattt aacctcacgt cgtccaatat
     3541 actttgaaaa cttattccct cgcttgccag acggcgatgc aatgtggaaa cagaaatgta
     3601 gagatatctc gctaccttgt ttgctgtcca ttttgttcta ggttcggata gcattaggtt
     3661 ataacaacga cttattaatg attgtttact atctgataaa agtaaacaat taacatgatg
     3721 tgctcctaat gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg ttgagaagcc
     3781 tcgggaaata gatgttttta gttgctccca acaatatatt aactcggggt tctgaggtaa
     3841 gcagaaacct gtcgtgttac gaatttgatc agttatcgca taaaattttt gaaacttttc
     3901 aattagatca atgggataat aaagcatttc tgcaagataa agacctgctt ctggataatt
     3961 cacaatataa aattcatatc cacagggaaa taatattatc tgattattat caacagttag
     4021 actatgcgtc tcccaattga taactttttt tccctgacgg atacgacata aagctggcat
     4081 aagaggctta accctgtgaa tctcatgatg tttatgcatc cgtatttctt caatctttag
     4141 gttagactta tctcttgcca gcatactctt accctatttt atctcataaa ctggtgttat
     4201 ctcagcggtt gcaattttat tggcattaag catataacca actaacgctc cgctggaatt
     4261 agaatccaca ggaatcttat cagtttttag agcccatact ttaaactggt aatgatgggg
     4321 tttatctcct ttgggaggac atgctccacc aaatcctgca taaccaaaat catttcggcc
     4381 ttgaacagca ccagtcggaa gttttgtacc atcacgtctt cctgcatcag tgggcaaata
     4441 tgttacagtt gctggaatat tagcaacagt ccagtgccac catccactgc ctgtaggagc
     4501 atctggatca tacacagtta cggcaaagct tttggtgcct tcaggagcac cagaccaggt
     4561 taatgagggc gatgtattac caccttcaca cccaaatcca gaaaatacat gagacgttgt
     4621 aagttgctct cctgttttta tttcattact agtgacctga aatgctgcgg cctgcgcaga
     4681 aaatgttatg aatgccaata cacttgaaac gataagtttt ttcataaaaa cctcattgtt
     4741 gtgacctatc gttattttat ttgatgtgcc ttgatctcat tatgcataaa ggcgcaatga
     4801 tcgcgcaaat gcaatcataa ttataaaggt gccccgaaca ttagccataa tatgcacaaa
     4861 tcagattcct gttatctcaa ggagaattat tttttaatgc ggtgcacttc gtgacaacaa
     4921 gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatagctg gcaggtccag
     4981 tcgttgacca aaaaatcccc aggaaagcaa actaatcaga acgataccga ctcctgacca
     5041 gatagcataa gcaatccctg taggtatata agccagcgtc tgagctaata accagaatga
     5101 tgcacaatag caaataattg tgccaacaga gggccataac cgtgtaaacc cctctgaaaa
     5161 cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat aaatataagg
     5221 gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt ttgaacacaa
     5281 ctcaccgttt tcatcctggt gtatgtgtat ccaaggggct ctggcgtggc aagagataaa
     5341 ggcaggaaca ataatgatca gaaggaaccc caggctaacg aatgagttca ctcttcctgg
     5401 gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc agaaaacaac
     5461 caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc catgccccac
     5521 gctgtgggat cggatataat taaacaagca tagtcaacag gttacacaac ctacacctgc
     5581 atggacatca cttcctgata cgccgccacc agcttattac gcacctgaat ccccatttgc
     5641 atagaaactg aggctttttg catatcggtc atcacatcgt ttagcgccac gccgggttca
     5701 ccgagggtga atttttctgc ctgcgtgcgg gcgactgttt gtgtatcgct tatgcgatcg
     5761 agcgcggcgt gcagctgccc ggcaaaacta atggtcggtt gcggcagtga ttcctgcgca
     5821 cgcgcactca tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct
     5881 gacattttcg tctcccggat aatttctggt aacaaagcct accagtaagt caataagaca
     5941 aaggcgctaa atagcaacaa aaaaactggt ttattggcgg atagaaaaaa cgaaagcaca
     6001 aataatggga gcgtcaattt ttcgagtgtg ctgacccgag agtgagtctt gttccacttt
     6061 gccaataacg ccgtccataa tcagccacga agtgcgcgat gaatgcgact gcagcccaga
     6121 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgtta
     6181 ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
     6241 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
     6301 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgag gttccggcag
     6361 ataaagttca cgaactgcgc ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt
     6421 tcgaactgct cgatcaggaa aagtttggta tcagccagtt cagcgaacag gtgaattatc
     6481 agcgggcgct ggaaggcgag ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg
     6541 cgcgcgtaca tctggcaatg ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt
     6601 ctgcatcggt gacggtaaat ctgttacccg gccgcgcact cgatgaaggg caaattagcg
     6661 ctattgtaca tctggtttcc agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg
     6721 tggatcaggg cgggcatctg ttaacccagt ccaataccag cgggcgcgat cttaatgacg
     6781 ctcagttgaa atatgccagc gatgtcgaag gccgtattca gcagcgtatt gaagcgatcc
     6841 tgtcacctat tgttggtaac ggtaatattc acgcctaggt cacggcgcaa ctggacttcg
     6901 ccagtaaaga acaaacggaa gaacagtatc gccctaacgg tgatgaatct catgcggcgc
     6961 ttcgttcacg ccagcttaat gagagcgagc aaagcggttc cggttatccg ggcggcgtac
     7021 cgggggcgtt gtcgaatcaa ccggcacctg cgaataacgc gccaatcagc acgcctccga
     7081 caaatcaaaa taaccgccag cagcaggcga gcaccaccag caatagtggg ccgcgtagca
     7141 cacagcggaa tgaaaccagt aactacgaag tcgatcgcac cattcgtcat accaaaatga
     7201 acgtgggcga tgtgcaacgt ctgtcagtcg cggtagtggt gaattacaaa accttgccag
     7261 acggtaaacc attgcctctc agcaacgaac agatgaagca aattgaagct ctgacccgcg
     7321 aggcgatggg cttttctgaa aaacgcggtg attcgctcaa tgttgttaac tcgccgttca
     7381 atagcagtga cgaaagcggc ggagcactgc cgttttggca acagcaagtg tttatcgatc
     7441 agttgctggc tgccggtcgc tggttgctgg ttgctggtac tgctggtggc gtggttgctg
     7501 tggcggaaag cggtacgccc gcagctaaca cgtcgcgccg aggcggtgaa gactgtgcag
     7561 caacaggcgc aggcccgcga ggaagtggaa gatgcggtgg aagtccgtct gagcaaagac
     7621 gaacaactcc aacaacggcg cgctaaccaa cgactggggg cagaagtcat gagccagcgt
     7681 atccgtgaaa tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg ccagtggata
     7741 aataacgatc atgagtaacc tgacaggcac cgataaaagc gtcatcctgc tgatgaccat
     7801 tggcgaagac cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag tgcaaaccct
     7861 gagcgctgca atggcgaacg tcacgcagat ctccaacaag cagctaaccg atgtgctggc
     7921 ggagtttgag caagaagctg aacagtttgc tgcactgaat atcaacgcca acgattatct
     7981 gcgttcggta ttggtcaaag ctctgggtga agaacatgcc gccagcctgc tggaagatat
     8041 tctcgaaacc cgcgataccg ccagcggtat tgaaacgctc aactttatgg agccgcagag
     8101 cgccgccgat ctgattcgcg atgagcatcc gcaaattatc gccaccattc tggtgcatct
     8161 gaggcgcgcc caggccgccg atattctggc gttgttcgat gaacgtctgc gccacgacgt
     8221 gatgttgcgt atcgccacct ttggcggcgt gcagccagcc gcgctggccg aactgaccga
     8281 agtactgaat ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg gcggcgtgag
     8341 aacggcagcc gaaattatca acctgatgaa aacgcagcag gaagaagccg ttattaccgc
     8401 cgtgcgtgaa ttcgacggcg agctggcgca gaaaatcatc gacgagatgt tcctgttcga
     8461 gaatctggtg gatgtcgatg atcgcagcat tcagcgtctg ttgcaggaag tggattccga
     8521 atcgctgctg atcgcgctga aaggggccga gcagccactg cgcgagaaat tcctgcgcaa
     8581 tatgtcgcag cgtgccgccg atattctgcg cgacgatctc gccaagcgtg gtccggtgcg
     8641 tctgtcgcag gtcgaaaacg aacagaaagc gattctgctg attgtgcgcc gtcttgccga
     8701 aactggcgag atggtgattg gcagcggcga ggatacctat gtctgataat ctgccgtgga
     8761 aaacctggac gccggacgat ctcgcgccac caccggcaga gtttgtgcca atggtcgagt
     8821 cggaagaaac cattattgaa gaggccgaac ccagccttga gcagcaactg gcgcaactgc
     8881 aaatgcaggc ccacgagcag ggttatcagg cggggattgc cgaaggtcgc cagcaaggtc
     8941 acgaacaggg ctatcaggaa ggactggccc aggggctgga acaaggtctg gcagaggcga
     9001 aggctcaaca agcgccaatt catgcccgga tgcagcaact ggtcagcgaa tttcaaacta
     9061 cccttgatgc gcttgatagt gtgatagctt cgcgcctgat gcagatggcg ctggaggcag
     9121 cacgtcaggt gatcggtcag acaccaacgg tggataactc ggcactgatc aaacagatcc
     9181 aacagttgtt gcagcaagaa ccgttattca gcgggaaacc acagctgcgc gtacacccgg
     9241 atgatctgca acgtgtggat gatatgctcg gcgcaacttt aagtttgcat ggctggcgct
     9301 tgcggggcga tcccaccctc caccctggcg gatgtaaagt ctccgccgat gaaggcgatc
     9361 tcgacgccag tgtcgccact cgctggcaag aactctgccg tctggcagca ccaggagtgg
     9421 tgtaatgacc acgcgcctga ctcgctggct aaccacgctg gataactttg aagccaaaat
     9481 ggcgcagttg cctgcggtac gtcgctacgg gcgattaacc cgcgctaccg ggctggtgct
     9541 ggaagccacc ggattacaat tgccgctcgg cgcaacctgt gtcattgagc gccagaacgg
     9601 cagcgaaacg cacgaagtag aaagcgaagt cgttggcttt aacggtcaac ggctgttttt
     9661 aatgccgctg gaggaagtgg aaggtgtcct gcccggcgcg cgtgtatatg ccaaaaacat
     9721 ttcggcagaa gggctgcaaa gcggcaagca gttgccgctc ggtccggcgt tattaggtcg
     9781 tgttctggac ggtagcggta aaccgctcga tggcctgccc ttccccgata cgacggaaac
     9841 cggtgcgctg attaccccgc catttaaccc gttgcaacgt acaccgattg aacatgtgct
     9901 ggacaccggc gtgcgcccaa tcaatgccct gcttaccgtc ggacgcgggc agcgtatggg
     9961 gctgtttgcc gggtcgggcg ttggtaaaag tgtgctgctg gggatgatgg cacgctacac
    10021 ccgcgccgat gtcattgtcg tgggtttgat tggtgaacgt gggcgcgaag taaaagattt
    10081 tattgagaat atcctcggtg ccgaaggacg tgcacgctca gtggtgattg ccgctccggc
    10141 ggatgtttct ccgctcctgc gaatgcaagg tgccgcctat gccacgcgca ttgccgaaga
    10201 ttttcgcgat cgtggtcagc atgtgttgct gattatggac tccctcaccc gctacgcgat
    10261 ggcccagcgt gaaattgcgc tggcgattgg cgaaccacct gccactaaag gttatccacc
    10321 gtcggtgttt gccaaattac cggcactggt cgagcgtgcc ggtaatggca ttagcggcgg
    10381 cggctcgatt accgcgtttt ataccgtgct caccgaaggc gatgaccagc aggaccccat
    10441 tgctgactcc gcgcgggcaa tcctcgacgg tcacattgtg ctgtctcgcc gactggcgga
    10501 agccgggcac tatccggcta tcgatattga agcgtcgatc agtcgcgcaa tgacggcgtt
    10561 gatcagtgag caacattacg cgcgagtgcg caccttcaaa cagctgttgt cgagttttca
    10621 gcgtaaccgc gatctggtta gcgtcggcgc gtatgccaaa ggcagcgatc cgatgctcga
    10681 taaagccatc gccctgtggc cgcagctgga gggctatttg caacaaggca tttttgaacg
    10741 cgcggactgg gaagcgtctc tccaggggct ggagcgtatt ttcccgacag tgtcataacc
    10801 caggagataa cggcagatgg cggaagaaca gctcaaaatg ttgatcgatt atcagaatga
    10861 atatcgcaat aacctcaata gcgatatgag tgccgggatg accagcaacc gctggatcaa
    10921 ctatcagcag tttatccaga cgctggaaaa agccattact cagcatcgcc agcaacttaa
    10981 tcagtggacg cagaaagttg acattgccct gaacagctgg cgagaaaaga aacaacgttt
    11041 gcaggcctgg cagacactgc aggaacggca atccacggcg gcactgcttg cagaaaaccg
    11101 cctcgatcag aaaaagatgg atgagttcgc ccagcgcgcc gccatgagga aacctgaatg
    11161 attcgcttag cgcccttgat taccgccgac gttgacacca ccacattgcc tggcggcaaa
    11221 gccagcgatg ctgcacaaga ttttctcacg ttgttgagcg aagcattagc aggcgagaca
    11281 acaactgaca aagcggcccc ccagttgctg gtagcaacag ataagcccac gacaaaaggc
    11341 gagccgctgg tcagcgatat tcttgccgat gcgcaacaag cggatttact gatccctgtg
    11401 gatgaaacac ttcctgtcat caacgacgaa caatccacat caacaccgtt aaccaccgct
    11461 cagacgatga cgttggctgc ggtggctgac aaaaatacga caaaagacga aaaagcggat
    11521 gatcttaatg aagacgtcac cgcaagtctg agcgcccttt ttgcgatgtt gccgggtttt
    11581 gacaatacgc ccaaagtgac tgatgcaccg tcaaccgtgt taccggcaga gaaaccaacg
    11641 ctcttcacaa aactgacttc tgcgcaactc actacagcac agcctgatga cgcccccggc
    11701 acaccagctc agccattaac accgctggta gcagaagccc agagtaaagc ggaagtcatc
    11761 agcacgcctt cgccggtgac cgctgacgcc agcccgctaa tcactccaca ccagacacag
    11821 ccactgccca ccgtcgccgc gcctgttttg agtgcaccgc tgggttctca cgaatggcaa
    11881 caatcattga gccagcatat ttcgctgttc acccgccagg ggcaacaaag tgcagagttg
    11941 cgtctgcacc cacaggattt aggtgaagtg caaatttccc tcaaagtgga tgataaccag
    12001 gcgcagatcc agatgatttc accgcatcag cacgtacgcg ccgccctgga agcagcgctg
    12061 ccggtactgc gtacgcagct ggccgaaagt ggcattcagt tagggcaaag caacattagt
    12121 ggcgaaagct ttagtggtca gcagcaggcc gcttcccagc aacagcaaag ccaacgcaca
    12181 gcaaaccatg aacctctggc gggggaagac gacgatacgc ttccggttcc cgtctcttta
    12241 caagggcgtg taacaggtaa cagcggcgtt gatattttcg cctaacgtca gaggtagcac
    12301 cgtaatccgc gtcttttccc cgctttgttg cgctcaagac gcaggataat tagccgataa
    12361 gcagtagcga cacaggaaga ccgcaacaca tgactgatta cgcgataagc aagaaaagca
    12421 agcgatcgct ttggatcccg attctggtat tcattaccct cgcggcctgt gccagcgcag
    12481 gttacagcta ctggcattcg catcaggttg ccgctgacga caaagcgcag caacgcgtcg
    12541 tgccctcccc ggtcttctat gcgctggata cctttacggt caatttgggc gatgcggatc
    12601 gcgtgcttta tatcggcata accctgcgac tgaaagatga agcaacccgc tcgcggctga
    12661 gtgagtattt gccggaagtc cgtagtcgct tgctgttact gttttcacgt caggatgctg
    12721 ccgtactggc gacagaagaa ggcaagaaaa acctgattgc cgagattaaa accacacttt
    12781 ccaccccgct tgttgccggg caaccgaaac aggatgtcac cgacgtgctg tataccgctt
    12841 ttattctgcg ataacgacat gggcgatagt attctttctc aagctgaaat tgatgcgctg
    12901 ttgaatggtg acagcgaagt caaagacgaa ccgacagcca gtattagcgg cgaaagtgac
    12961 attcgtccgt acgatccgaa tacccaacga cgggttgtgc gcgaacgttt gcaggcgctg
    13021 gaaatcatta atgagcgctt tgcccgccat tttcgtatgg ggctgttcaa cctgctgcgc
    13081 cgtagcccgg acattaccgt cggggccatc cgcattcagc cgtaccatga atttgcccgc
    13141 aacctgccgg tgccgaccaa cctgaaccta atccacctga aaccgctacg cggcacaggg
    13201 ctggtagtgt tctcgccgag tctggtgttt atcgccgtgg ataacctgtt tggcggcgat
    13261 ggacgcttcc cgaccaaagt ggaaggtcgc gagtttaccc atactgaaca gcgcgtcatc
    13321 aaccgtatgt tgaagctggc gctggaaggc tatagcgacg cctggaaagc gattaatccg
    13381 ctggaagttg agtacgtgcg ttcggaaatg caggtggaat ttaccaatat caccacctcg
    13441 ccgaacgaca ttgtggttaa cacgccgttc catgtggaga ttggcaacct gaccggcgaa
    13501 tttaatatct gcctgccatt cagcatgatc gagccgctac gggaattgtt ggttaacccg
    13561 ccgctggaaa actcgcgtaa tgaagatcag aactggcgcg ataacctggt gcgccaggtg
    13621 cagcattcac agctggaact ggtggccaac tttgccgata tctcgctacg cctgtcgcag
    13681 attttaaaac tgaaacccgg cgacgtcctg ccgatagaaa aacccgatcg catcatcgcc
    13741 catgttgacg gcgtcccggt tctgaccagt cagtacggta ctctcaacgg tcagtatgcg
    13801 ttacggatag aacatttgat taacccgatt ttaaattctc tgaacgagga acagcccaaa
    13861 tgagtgacat gaataatccg gccgatgaca acaacggcgc aatggacgat ctgtgggctg
    13921 aagcgttgag cgagcaaaaa tcaaccagcg aaaaaagcgc tgccgacgcg gtgttccagc
    13981 aatttggcgg tggtgatgtc agcggaacgt tgcaggatat cgacctgatt atggatattc
    14041 cggtcaagct gaccgtcgag ctgggccgta cgcggatgac catcaaagag ctgttgcgtc
    14101 tgacgcaagg gtcagtcgtg gcgctggacg gtctggcggg cgaaccgctg gatattctga
    14161 tcaacggtta tttaatcgcc cagggcgaag tggtggttgt tgccgataaa tatggcgtgc
    14221 ggatcaccga tatcattacc ccgtccgagc ggatgcgccg cctgagccgt tagtgatgaa
    14281 taaccatgct actgtgcaat cttccgcgcc ggtttctgct gagccactgc tgcaggtgag
    14341 cggcgcactc atcgccatta ttgccctgat cctcgctgct gcctggctgg taaaacggct
    14401 gggatttgcc cctaaacgca ctggcgttaa cggtctgaaa attagcgcca gtgcttcgct
    14461 gggcgcgcgt gaacgggtcg tggtggtcga tgtggaagat gcacggctgg tgctcggcgt
    14521 taccgcaggg caaatcaatc tgctgcataa acttccccct tcagcaccaa cggaagagat
    14581 accgcagacc gattttcagt cggtcatgaa aaatttgctt aagcgtggcg ggagatcctg
    14641 atgcgtcgtt tgttttctgt cgcaccggtc cttctctggc tgattacgcc cctcgccttc
    14701 gcgcaactgc cgggtatcac cagccagccg ctacctggcg ggtatcacca gccagccgct
    14761 acctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt
    14821 gacgttcatt ccagcgattt tactgatgat gaccagtttc acccgcatca ttattgtttt
    14881 tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg tattgctggg
    14941 gctggcactg tttttgacct tttttattat gtcgccagtg atcgacaaaa tttatgtcga
    15001 tgcgtaccag ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg aaaaaggggc
    15061 gcagccgctg cgtgagttta tgctgcgtca gacccgcgag gcagatttag ggttgtttgc
    15121 cagactggcg aataccggcc cgttgcaggg gcctgaagct gtgccgatgc gcattttgct
    15181 cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca cgattttcat
    15241 cccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg ggatgatgat
    15301 ggttcccccc gccaccattg ctctgccctt taaactgatg ctgtttgtac tggtggatgg
    15361 ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag gcaaaatgac
    15421 acctgaatcg gtcatgatga tgggaactga agcgatgaaa gtcgcgctgg cactggcagc
    15481 cccgctgttg ctggtagcgc tggtcacggg ccttatcatc agtattttgc aggcagccac
    15541 gcagattaac gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat ttatcgccat
    15601 cattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca ccttgttcac
    15661 taacctgccg tatatcatcg ggtagcccgt acaatgatgc aggagacaag cgatcaatgg
    15721 ctttcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct catttctacc
    15781 gcgccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct ggcaatgatg
    15841 atcacgttcg ccattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt
    15901 gctctgtggc tggccgtaca gcagatcctg atcggcattg cgcttggttt taccatgcaa
    15961 tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca
    16021 tttgcgacat ttgtcgatcc tggcagccat ctcaatatgc ccgttttagc gcgcatcatg
    16081 gatatgctgg cattactgct gttcctgaca tttaacggtc atttatggtt gatttcactg
    16141 ctggtcgata cctttcacac cctgccgatt ggtagcgaac cgttgaacag caatgcgttt
    16201 ctggcaccca ccaaagcagg gagtttgatt ttccttaacg ggctgatgct ggcgttaccg
    16261 ctcattactc tgttgctgac cctgaatctg gcattaggtt tactgaatcg tatggccccg
    16321 caattatcca tttttgttat tggatttcca ttaactctga ctgtcggcat ctctttaatg
    16381 gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga aatttttaat
    16441 ttgctggctg atattattag tgaattacca ttaatataat tccgtaacgt ttatcatgct
    16501 accctaagga ttatccgaaa aataatatct gcgaacatct tccaggatac tcctgtagcg
    16561 aaatatttgt tttaagctca ctcacatatc gcaacattta ctttaagaca attccaggca
    16621 aattatacaa cactttacgg gatagtaagt ccgcctgaaa aattgcgaga gtggcgcatt
    16681 aggtgaccca tgttgttccg tttagtcatg atgaaatatt caggtaaggg gaattatcgt
    16741 tacgcattga gtgagggtat gccatgtcaa cgattattat ggatttatgt agttacaccc
    16801 gactaggttt aaccgggtat ctgttgagta gaggggttaa aaaaagagaa atcaacgaca
    16861 ttgaaaccgt tgatgacctt gccatagctt gtgattcaca gcgtccttca gtggtgttta
    16921 ttaatgagga ctgtttcatc cacgatgctt ctaacagtca gcgcatcaag cacatcatta
    16981 atcaacatcc caatacgtta tttatcgttt ttatggcaat tgccaatgtt cattttgatg
    17041 aatatctatt ggtcagaaaa aatttattga tcagttctaa atcgattaaa ccggaatctc
    17101 tcgacgatat ccttggcgat attctgaaaa aagagacaac gataacctcg tttttaaata
    17161 tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc
    17221 agggaaccat tcaaatctcc gaccaaatga atatcaaagc caagaccgtt tcatcgcata
    17281 aaggtaatat taaacgtaag atcaaaacgc ataataaaca ggttatctac catgtcgtcc
    17341 gactgacgga taatgtgact aatggtattt ttgtcaacat gcgctaacac attctgactg
    17401 gtggtttccc accagtcagg ctgaataaga ttactctgct ttctcaacaa agataccgtc
    17461 ctgatgccct gcttcattaa agaaccagat gccgagcggg tagtcttcca gcgcaaccag
    17521 gtacattgtg ccttcactaa actcctcaac tgccagtacc actccaggac gacgcggacc
    17581 gccatccgtt ttgactgtta cccgatcatt caccttcatc gttttcctcc tgtggctttg
    17641 tgccagtgta gaacaatttc gtttttctgg cagcgccaag cgcgcgcgag tgctgatttt
    17701 ctcgacggtc tatacttaag agatgccagg cggacttaac gactggcggc aacaacagag
    17761 taacggttgc gaggaaagat gatgaaaacc gcaaaagtgt acagcgatac cgcaaaacgt
    17821 gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca atgaaattag cgaaagcgaa
    17881 gttcatcgca gccagaacga ttctgaacac gttagcgtcg atggacgtga atatcataca
    17941 tggcgtgaat tggcggatgc cttcgaactg gatattcatg acttcagcgt ctctgaagtg
    18001 aatcgttgaa tgcacaataa aaaaatcccg accctgaggg tgtcgggatg aaacttgctt
    18061 aagcaagaag cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat
    18121 ctgcaatttt tttcgctgac aagaaaatat tcgcgctatg aatgattaac gctacttatg
    18181 aatagaaatg tgacgcgcgt caccccttgg tggctctggt gtcaacggga tcgctcccga
    18241 aaaaatcatc tttgattatt tgtgctgttt tgagaatcat cctggtaagg ggagtactgc
    18301 gcgaccatgt tttcaattca acaaccacta ctggttttta gcgatcttga tggcaccctg
    18361 ctggacagtc atagttatga ctggcaaccg gcagccccct ggctcagccg tttacacgaa
    18421 gccaatattc ccgtcattct ctgtagcagt aaaactttag cggaaatgct gtacttgcaa
    18481 aaaatgttgg ggctacaagg cttaccgctg attgcagaga atggcgcagt gatccagctt
    18541 gctgaacaat ggcaggatat agacggtttt ccacgcatca tctcaggtat tagccatggc
    18601 gaaatctgcc aggttttaaa tacgctacgc gagaaagaac attttaaatt cacgactttt
    18661 gatgatgtcg acgatgcaac cattgccgaa tggacgggat taagccgtag ctaggcggcg
    18721 ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg
    18781 gcacaattta tcgctcgtct gaacgaactg ggcttgcagt ttatgcaggg tgcgcgcttc
    18841 tggcacgtac tggatgcctc tgccggaaaa gatcaggctg ccaactggat tatcgcgacc
    18901 tatcaacaat tgtcaggcag acgcccaacc acacttggcc tgggcgatgg gccaaacgat
    18961 gcgcctttac tggaggtaat ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg
    19021 gtacatctgc atgatgagga tccggcccgc gtctggcgaa cgcagcgtga aggaccggaa
    19081 ggctggcgtg aagggctgga ccattttttc tccgcccgtt aagcgttatc gctcgcgaat
    19141 acccgattgc gcccggcctg ttttgcgagg taaagccgac ggtcggccaa tgactggagt
    19201 tgttcaaaat cataatcacc ggtttcctcg ctgctactta cccccagcga ggcactgatg
    19261 cgtatcgttg tactcttggc gatcagcatc tctttttcat ttaacttcag gcgaatacgt
    19321 tctgcgacct ccgcagcctg cgtcagattc gcgcctggca gaatcacaca aaactcctca
    19381 ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa tccggcagca
    19441 tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg
    19501 tcaagatcga cctgaatgac agaaaaagga tgttggtgcg tctgacacaa tttagcgagc
    19561 ggacgggctt tttcgaacag tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac
    19621 gcctgccact gcaacgagct ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca
    19681 taccaggaga ggagtaacat ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg
    19741 atactgccga aatcgccgcg cacgccttcg cttagcgtat ggacgcgcgc cagcacaccg
    19801 tcgaaatggt ccagacgttc ccagctaaca tagcgactgt ccatacgaat gccgccccgc
    19861 gtgtcatgtt ccattgcctg cgccagcaag gccagttcac gaggatcaaa aatattccct
    19921 gttggatggt caggattgga agaggtcaaa aatctcagct tactgtcata gagctgatac
    19981 tcaccatcga ggtttttatc gatggcgttt cttaaaaatt gctgcatagt acgcacggga
    20041 atactcatcc ccagcacgcc ataccagtag ttattactgt ctaccggaac actgacggta
    20101 acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg
    20161 tgacgatttt ctcgttgcga atggccgata aaccagggtt gggtgacata gccgtaataa
    20221 cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa tccagcacgc
    20281 gagacataca tcgcctgttc aaccatcgac gaggagttgt gcgccagtcg cagcaagtaa
    20341 ccaacttcca gtgcagcggt aatttcattg tcgaggcttt cattttcgcg agacaggaaa
    20401 ttcccctcgc tgactaatgc atccgatacg ccattgactg gcagggtgcg ccgtcggttg
    20461 agttcgattt gccaggcgtg ctcgtcgcga tgctgttcga actcggttac ggcattacgc
    20521 agagaggtga aatccagtgg cgccacgaga gcttcgcgca tgccattacg caaaaagatc
    20581 agtttgtcga cgttatactg caaatgctta tcgagcgcgt tggctacgtt ttccagatga
    20641 ttacgctagc tggagatata ggcatcttcc agcaccacca cttcacgcca ggtgagcaag
    20701 gtggaaaaaa gcaataccac gataaagcag agattaacga catgaccggg ccccaggcgg
    20761 cgtgcgagtt ttttcaacca gctctggttt tccatttttg tctcgtgctg caccctgacg
    20821 actcccaacc tgctttttta tgattctggc ataacccggc aatagcgtac cgcaaaacag
    20881 cgataatttt ataatggtgg catgataaga cgcgactatc attcattgca ggcattggga
    20941 caccaggccg gatgtgatcc ggcctggtgt taatgatacc ctttcaaact tcttccgcag
    21001 aacgactccc ctcccccgga acaagatcgg tttcatgaaa agcttcccgc ttgaccccgt
    21061 aaccgtcata ccaacgacac tcaaccatac cgctggagta tccagtgaca atcatccgcg
    21121 ggccgccctc tttaaccgta acttcctcac taaccataaa gctcatactc gcctcctttt
    21181 ttcgagtgaa acttgttcac cttagttgaa gatggcgaat tttgcataat agccagtgcg
    21241 agtattaatg tgcctgaccg cgcattttcg ctactgcttt cactcccagc accacgatgc
    21301 cgccgatgat aaatccaaga atcagattta aaacagtcgg taatatcatc gccaccactg
    21361 cactttgctg cccggcgata tgttcaatag cgtgatgcag cggcgcaata ccatgtacca
    21421 caatcccgcc gccgacgagg aacatcgcca gcgtgccgac aatcgataat gctttcatca
    21481 gccaaggcgc gataatcaat aaccctttac ctaatgcctg catcagcgcg ctagattttt
    21541 ccgccagcca ataccctagg tcatcaatct taacgataac ccctaccaga ccgtagacgc
    21601 ccacggtcac taccagcgcg atgcctgaaa gcaccagcac ctgattaagc aacggcgcct
    21661 ctgccacaat ccccagcgtg atggcgacga tttccgcaga caaaataaaa tcggtacgaa
    21721 tcgccccttt aattttgtcc ttttcaaact tcagcggatc ctgcgccgcc agcttctcca
    21781 gacgctgctg gctctgcgct ggatcttctt tatgtttacg cgcctccagc atatgcagca
    21841 ctttctctac cccttcaaag cagagaaacg cgccaccaat catcaacagt ggcgtaatcg
    21901 cccacgggat aaacgcactg atgatcagcg ccagcggcac cagaatcact ttattgatca
    21961 gcgatccttt cgccacgccc cagaccacgg gaagttcccg gttggcccgc acgcctgaaa
    22021 cttgttgcgc attgagcgat aaatcatccc ctaatacacc ggcggttttc ttcgccgcca
    22081 gtttgcccat cacagagata tcgtccagca gtgtggcgat atcatcgagc aacgtcagta
    22141 aactactccc ggccaaaagc attccctctc atttttattg ttgaagggtg taagtatgaa
    22201 gcaaaaacgg cagctctgat acttgcttca caggtcaaca tttatttaac aatcaagtgc
    22261 aattatatct ctcgccagca gaatgatttt cacgtttact atgaaccacc tttttatttc
    22321 cgccgtgagg gattatgcgt ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt
    22381 tgtatatcat ttggggctca acctattttg tcattcggat tggcgtggga agctggcctc
    22441 cgttaatgat ggcgggcgtt cgattccagg cagccggtat tttattgctg gcatttttgc
    22501 tactgcgcgg acacaaactt cccccgctac gtccgctgct caatgccgcg ctgattggcc
    22561 tgttattgct ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa aatgttcctt
    22621 ccggcatcgc cgccgtagtg attgcaaccg tgcccctctt taccctgtgc ttcagtcgcc
    22681 tgtttggcat taaaacgcgc aaactggaat gggtgggtat tgccattggg cttgccggaa
    22741 tcatcatgct caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg attctgattt
    22801 taatcggctc gattagctgg gcgtttggct cagtttatgg ctcgcgcatt accttacctg
    22861 tagggatgat ggcgggtgcg attgagatgc tggcggcagg cgtggtgtta atgatcgcat
    22921 cgatgattgc gggtgaaaaa ctgacggcgc tcccttccct gtcaggcttc cttgcggtcg
    22981 gctatctggc gctgtttggt tcgattatcg ccattaacgc ttatatgtat ttaatccgta
    23041 atgtcagtcc ggctctcgcc accagctacg cctacgttaa cccggtggtc gcggtcttgc
    23101 tgggtacggg actgggtggc gaaacgctgt cgaagattga atggctggcg ctcggcgtaa
    23161 ttgttttcgc ggtggtactg gtcacgttgg gaaaatatct cttcccggcc aaacccgttg
    23221 tcgcgccagt tattcaggat gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc
    23281 gcgctggcac cttcgccgca gatccactct tccagacgct cggtaagcgc cgcatccgtc
    23341 agcttctcgc gcctacgtaa cgcacactcc cagacaatca gtacgcgcca gccgagctcc
    23401 tgcaagcgac tgatatcgcg gcgatcgcgt tcaacatttt tacctatctt ctccagccag
    23461 aactcagttc gagtcgcagg cactttaaac agatagcagt gatgatgatg ccagaagcag
    23521 ccgtgggtaa atatcacgca gcgatattca tcaacgacaa aatccgggcg tccgggcaga
    23581 ctggcgtcct gaacgcgaaa cgcaaggccc tgcccggtta acaaactggc gaggcgcttc
    23641 tcaatcgccg tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg
    23701 tgaacgtcga ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt tgatttttgg
    23761 ctcaagcagt tttgccaccg cggcaaagac cggcacgacc accgagttgc cgaactggcg
    23821 ataggcctga gtgtccgaaa ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc
    23881 cattaagcgc gcgcattcac gaggcgttaa ccgacgtgga cgatgttgct gattcagcgg
    23941 atcgtcaaag tctttctcac ccgtggccat atcccaaccg cgatcgatta aaatttccgc
    24001 gccatctttg taataacgag cagacagcgt acgggtgacg ctttgcggat tgttcggata
    24061 aaccattcca taaccgaagc cgttaccgcg cgcctgatgt tttttcgcat atcgatagag
    24121 gtacttccac agcaccggcg tcaggatata tttcgcctca accatcgggt ccagcagctg
    24181 cgccagcgtc actcgctgtg cagggaaaca ttcgctgata tcacgcagag taaaatcggc
    24241 tttcagattc agatcgcgac gaaaacccac cagcacgatg cgttcacggt gctgcggcag
    24301 gaaatgttta ccatcgatga ttttcggatc gtccggcccg ttatcttctg catcggcaac
    24361 gtcatagccc agttcgtcca gcgtctgcat gatgatgcgg aacgtttttc ccttatcgtg
    24421 actcttcagg tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat
    24481 gcgcaccaca tcaaagaaca gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc
    24541 gagcgagttc tttttcgata cgccagccag cgaaaatggc tgacaaggga aaccggccag
    24601 taaaacatcg tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct catcactcac
    24661 gccttcttga tgactgaggg tgatgtcgcg gatatcttca ttaaaatgat gcgtcgccgg
    24721 gtcgcaataa tggttggctt tataagtgcg taccgcatgt ttgttccatt cgctggtaaa
    24781 cacgcactgt ccaccaatcg attcaaaacc acgacggatg ccgccaattc cggcaaatag
    24841 atcgataaag cgaaacgcat aatgcggatg atgttccggt ggtttgggta acaacgtttg
    24901 cagatgggca aactctttct cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg
    24961 ttttaaaatt gccgcgctcc agtgattctc acccacacca ttaagctgcg ccaccagcat
    25021 tttaacatca taaatttgca gcagtttctc cagcattgcc tgtgcggcat tcccggtgct
    25081 gtatgaatcg gttactgata tattttcctg catagattta accggccatc tgagatggcc
    25141 gacaggttaa cataattact gccgtttagg gccacgcgct cagccagcgg gtgaaaacgc
    25201 atctatcacc ttgtgatcga taccttcatt ctcccccgcc agttcggcac cgagcttcgc
    25261 catcaaacag cgccaagctc aatgcccctg aaacggcaaa tacgcgcgcc aggccaatcg
    25321 cccccagggc ttccagccga tccgcatcct ggacgatttt agcttccgtt gttaaggggg
    25381 cgatttgcgc gctgaaactg tgagcggcaa tggcatgaca aacggcctca attttctccg
    25441 ccggaaattg cacgaactct tcacggagca ggcgacgcgt ctcttctgcc gccaggattg
    25501 aagaacgctg ccgttgcgga tgatttttcg ccaggctcac gatatcgtga aaataacagg
    25561 cggttaaaat caccagcata tcgacatcgt catctgccgc cagtttctgt gccgtagccc
    25621 agacacggcg aaaatgacac aggtcatgcg ccgcgtcctg atgttgatga tggtttttca
    25681 accagttctc gaactatgcc tgccagtgtt gtaactccat acgcgctccg ttgccaaaga
    25741 gtctcaacct tagcagtttt tattcatagc gtctgcaacg tgcggaacga ggtcgggtgt
    25801 accatgcaat gccgcctaac aaggcaccaa ccagggccat aaacagaaat ccgaccaatg
    25861 ctcctaacca cttccccgcg gtagatccta aatcactttc aaatcccgac acgggggcat
    25921 cggggatccg gctcatcacc cagatgtatc gcgccattgc ccagacgaaa tagccacacc
    25981 atgtatagaa agccc
//
LOCUS       DAJVQR010000054        25897 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVQR010000054 DAJVQR010000000
VERSION     DAJVQR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..85
                     /gene="ldrD"
                     /locus_tag="OQM52_002377"
     CDS             <1..85
                     /gene="ldrD"
                     /locus_tag="OQM52_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCS1564196.1"
                     /translation="AFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(172..1851)
                     /gene="bcsG"
                     /locus_tag="OQM52_002378"
     CDS             complement(172..1851)
                     /gene="bcsG"
                     /locus_tag="OQM52_002378"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1564197.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1848..2039)
                     /gene="bcsF"
                     /locus_tag="OQM52_002379"
     CDS             complement(1848..2039)
                     /gene="bcsF"
                     /locus_tag="OQM52_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1564198.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2036..3607)
                     /gene="bcsE"
                     /locus_tag="OQM52_002380"
     CDS             complement(2036..3607)
                     /gene="bcsE"
                     /locus_tag="OQM52_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1564199.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3880..4068
                     /locus_tag="OQM52_002381"
     CDS             3880..4068
                     /locus_tag="OQM52_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1564200.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4080..4832
                     /gene="bcsQ"
                     /locus_tag="OQM52_002382"
     CDS             4080..4832
                     /gene="bcsQ"
                     /locus_tag="OQM52_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1564201.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4829..5188
                     /locus_tag="OQM52_002383"
     CDS             4829..5188
                     /locus_tag="OQM52_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS1564202.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5185..7446
                     /gene="bcsA"
                     /locus_tag="OQM52_002384"
                     /pseudo
     CDS             5185..7446
                     /gene="bcsA"
                     /locus_tag="OQM52_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7457..9796
                     /gene="bcsB"
                     /locus_tag="OQM52_002385"
     CDS             7457..9796
                     /gene="bcsB"
                     /locus_tag="OQM52_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1564203.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9803..10909
                     /gene="bcsZ"
                     /locus_tag="OQM52_002386"
     CDS             9803..10909
                     /gene="bcsZ"
                     /locus_tag="OQM52_002386"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1564204.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10986..11327
                     /locus_tag="OQM52_002387"
     CDS             10986..11327
                     /locus_tag="OQM52_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS1564205.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11242..11493
                     /locus_tag="OQM52_002388"
                     /pseudo
     CDS             11242..11493
                     /locus_tag="OQM52_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11575..13563
                     /gene="hmsP"
                     /locus_tag="OQM52_002389"
     CDS             11575..13563
                     /gene="hmsP"
                     /locus_tag="OQM52_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1564206.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13753..15039
                     /gene="dctA"
                     /locus_tag="OQM52_002390"
     CDS             13753..15039
                     /gene="dctA"
                     /locus_tag="OQM52_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1564207.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15260..16756
                     /locus_tag="OQM52_002391"
     CDS             15260..16756
                     /locus_tag="OQM52_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1564208.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16852..17781)
                     /gene="kdgK"
                     /locus_tag="OQM52_002392"
     CDS             complement(16852..17781)
                     /gene="kdgK"
                     /locus_tag="OQM52_002392"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1564209.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18013..18780
                     /gene="pdeH"
                     /locus_tag="OQM52_002393"
     CDS             18013..18780
                     /gene="pdeH"
                     /locus_tag="OQM52_002393"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1564210.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18850..20910
                     /locus_tag="OQM52_002394"
     CDS             18850..20910
                     /locus_tag="OQM52_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1564211.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21092..22414)
                     /locus_tag="OQM52_002395"
     CDS             complement(21092..22414)
                     /locus_tag="OQM52_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1564212.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22825..23838)
                     /gene="yhjD"
                     /locus_tag="OQM52_002396"
     CDS             complement(22825..23838)
                     /gene="yhjD"
                     /locus_tag="OQM52_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1564213.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23887..24786)
                     /locus_tag="OQM52_002397"
     CDS             complement(23887..24786)
                     /locus_tag="OQM52_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1564214.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25306..>25897
                     /locus_tag="OQM52_002398"
     CDS             25306..>25897
                     /locus_tag="OQM52_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1564215.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLN"
BASE COUNT         6168 a         6503 c         7050 g         6176 t
ORIGIN      
        1 ggccttctgg catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt
       61 gaactggctg aataagcgga agtaacgtgt catgcgggcg tcaggctgcc gtaatggcaa
      121 tttgcgcccg gatcaggccg cagaggttaa actctgcggc ctttttcgtg cttactgcgg
      181 gtaaggcacc cagtcgccgc cgttcaggcg aacgtacggt ttatcttggt attgaataac
      241 tactgcattt gagttctcgg agaccggtgc tgtttgtggc aacccactgg tgagtttttt
      301 ccagtcaaca ttgtcttcgg tgaaaatctt gccatcaaga acgcgaacca ccagatcgga
      361 gatagccagg aagctgctcg gttggtcaat gacaatcggt gccccctgat gtggtgcctt
      421 catgccgaag aatttcaccc caacggggac gtctgtgata gacgggctag ggatatcacg
      481 taggccagat acctgcattc tgtcgccctt cagcgcgccg ccgtgttccg gcaccacgac
      541 caccatcact ttacgacccg atttctccag ttcagtaaag aaggcgtcca gttcatcaaa
      601 gaatttctgc gcccgcgctt tgtaatccgc tgttttgctg acccccggat aatggttgcc
      661 gtcatgcagt ggaagcgtgt tgtagaacgt ggcactacgg ctgtttttat ctttttcggt
      721 aacgtccagc cagcggttaa gcacggcggt atcgtcataa accggcgaac catcaaagcc
      781 cagcaaaata accggcagat ttgtttgatc catcaattca gtctgcatgc cgccattttc
      841 gcgaacttct ttcaaaaaac cgccgaactg gccgttatgt cccatcatca ggtgctgggt
      901 aaagcccagt ttcgaaaggt tatcaaacag atagcagtcg ttatttgccg gttgatacag
      961 attagtgtgc gaagtctgcc cgcagctggc gcgcagtaaa cggatcgccg ccgggccact
     1021 gtaggaggtg gcggagttaa agttcttgaa atcaatatcg aaatgcgacc acagtggatg
     1081 cgacatcaac ccggcggctt ctatatccga ccaggaaagc gaacagatgt taatcaccag
     1141 tagttcaaat ggctgagcat cagcgggcag cgaagacggg aaggtcgatt tacgtttcgc
     1201 ctccgcgtta tagaaattat tcagccaggc gttaaggttc gccgttgttg gcggtgcagt
     1261 ttgtgcgggc atatcaccca ctaccggtgc gccacccgtc gccgcaacgg ttgccgctgc
     1321 gttaccaccc gtcgttgtta cagtggtcgt cggttgtccg gctggccaca aggagaaact
     1381 tggtcccgcc agggtaagta cgttcagcca tagcagtatg gcaaccacaa aaacggtaat
     1441 gcgaatccat tgtgacagga ataaccaggc cactaataaa acaaaaatgg ccccaatcat
     1501 ctgccagtta ataaagcgtg tgacaaggtc gattaaataa tcggtactga accccgccac
     1561 ctgcgaaccc tggctcatta tgctttccgg gccaggcaac cagatgtcat gccagaacaa
     1621 agcaaagccg atcggcaggg caatccagtg gcgcaagcga tgcaggctgt aacgcggaat
     1681 gggcatcagc agaaacgcgg caaacaccaa attgaggagc gggtggaagt taagatatcc
     1741 cgcccacaac aggccaaact taaccagaaa atagaagttc cagccggaaa ggccgcgcca
     1801 gtattgccag agggaagaag gcatggcggt attttgcgta aattgagtca ttttttggtt
     1861 gccctggctt tttccgtgcg gcgtaacgtc ccggccggtt taacataacg aggtttagca
     1921 aagaacaaac gtaaggtgtc gcgaatgcgt cgcaaagagt gccgcgccag atagcccagc
     1981 gggaaaaata tcagtgcgca aacgataata atttcaatga tatcgctgat ggtcatcatg
     2041 atgagcgctc cacagcatca tctaacagtc gcatgggttc tggtattcgt cgccagtggc
     2101 gaccatcgtg ctcggcattg atgaccggtt tagaactttg cgttaaaggc agcggcattc
     2161 cccattgttc tggggcaagc aggcgcatct gcaccagctc ggcactgatt tgatcatctt
     2221 caaaccagac catacggttt gagaaaatgt cgccagtagg caatgggaaa atatgattca
     2281 acgcggtatc cagatcgtta atccgacaga atgagagaaa cagcaccagc cgattaccgc
     2341 caatggtcat gatatcgccg gtacggttag ggcgacatag cgtcagggct tgctcaacgc
     2401 ggatacccgg taccggacgt agggcaacca gaacgccttt accgtgggca ggcaagagag
     2461 ggttattcat catgttgttg acggcattac agaacacatc ccacttctgg aaaccacgca
     2521 gtttgagcgg ctgggtcatt gacagcaagg tagtgatatc ttccggtaca tagcgactaa
     2581 acttctgccc ttgcacgctt tcgatcatcg tcagacaacg ggagagtggc gcattccacg
     2641 gaataaccat atttgcaccg caggccaata acaaacgttc atcggtggcg cgcaggctag
     2701 cggtattttc ccgcacgagg attttcatcg cactaccgcg ctggcgacgc agggtatgaa
     2761 tgctgcgggc cagtggctcg atttgcgcat tttgctgtaa agaaaagacc accgtcgccg
     2821 cctgagcggt acgggcttca ttgaacagga cttcgttatt gttgaacagt tgccagtgtt
     2881 ccgatagcgg cggcgcacct tccagtacag caacattact cagaatgcgt ttttcgtcgc
     2941 tgcgtggttg gatctccgcc tcttcgcttt gaactaatgt ccagatacca ttttgttgct
     3001 gaacgctaag ctgctgacgg gcgctgaccc ctttttcgtt gcaccagaag gcaatatcca
     3061 gcaaatgttg gtcaccctga aaacgcaaac tggcaagacc aaaaagtgaa cggtattcct
     3121 caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac
     3181 aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag
     3241 gaatattttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa
     3301 tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa
     3361 ataattttat tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc
     3421 tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg
     3481 ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca
     3541 tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt
     3601 ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag aaataaacct
     3661 accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag gtcacaagac
     3721 gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt
     3781 gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt
     3841 ctcatgaatg gtacggttat ttcataggga tcaagcaaaa tgaataacaa tgaaccagat
     3901 actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc gttaaagcag
     3961 gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca gttggccgcg
     4021 gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg gattggtgaa
     4081 tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg
     4141 cattagcctg gtcattacaa atgttgggag aaaatgtcct ggtggtcgat gcctgcccgg
     4201 acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc tgggccagag
     4261 cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt
     4321 tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac tggcaaaccc
     4381 ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga
     4441 ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg
     4501 agcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg catcagcaag
     4561 cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg
     4621 acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc
     4681 gtgatgaagc gatggctgaa tgcctggcgg ctaagcaacc agtaggtgaa tatcgcagtg
     4741 atgcgctggc ggctgaagag atactgacgc tggcgaactg gtgcctgttg aactactccg
     4801 ggctgaaaac gccagtcggg agtgcatcat gagtatcctg acccggtggt tgcttatccc
     4861 gccggtcaac gcgcggctta tcgggcgtta tcgcgattat cgtcgtcacg gtgcgtcggc
     4921 tttcagcgcg acgctcggct gtttctggat gatcctggcc tggattttta ttccgctgga
     4981 gcacccgcgc tggcagcgta ttcgcgcaga acataaaaac ctgtatacgc atatcaacgc
     5041 ctcgcgtccg cgtccgctgg acccggtccg ttatctcatt caaacatgct ggttattgat
     5101 cggtgcatcg cgcaaagaaa cgccgaaacc gcgcagcggg cattttcagg tctgcaaaat
     5161 attcgtggac gttaccatca atggatgaac gagctgcctg agcgcgttag ccataaaaca
     5221 cagcatctgg atgaaaaaaa agagctcggt catttgagtg ccggggcgcg gcggttgatc
     5281 ctcggtatca tcgtcacctt ctcgctgatt ctggcgttaa tctgcgttac tcagccgttt
     5341 aacccgctgg cgcagtttat cttcctgatg ctgctgtggg gggtagcgct gatcgtacgg
     5401 cggatgccgg ggcgcttctc ggcgctaatg ttgattgtgc tgtcgctgac cgtttcttgc
     5461 cgttatatct ggtggcgtta cacctctacg ctgaactggg acgatccggt cagcctggtg
     5521 tgcgggctta ttctgctctt cgctgaaacg tacgcgtgga ttgtgctggt gctcggctac
     5581 ttccaggtag tatggccgct gaatcgtcag ccggtgccat tgccgaagga tatgtcgctg
     5641 tggccgtcag tggatatctt tgtcccgact tacaacgaag atctcaatgt ggtgaaaaat
     5701 accatttacg cctcgctggg tatcgactgg ccgaaagaca aactgaacat ctggatcctc
     5761 gatgacggcg gcagggaaga gtttcgccag tttgcgcaaa acgtgggggt gaaatatatc
     5821 gcccgcacca ctcatgaaca tgcgaaagcg ggcaacatca acaatgcgct gaaatatgcc
     5881 aaaggcgagt tcgtgtcgat tttcgactgc gaccacgtac caacgcgatc gttcctgcaa
     5941 atgaccatgg gctggttcct gaaagagaaa cagctggcga tgatgcagac tccacaccac
     6001 ttcttctcgc cggacccgtt tgaacgcaac ctgggacgtt ttcgtaaaac gccgaacgaa
     6061 ggcacgctgt tctatggtct ggtgcaggat ggcaacgata tgtgggacgc cactttcttc
     6121 tgcggttcct gtgcggtgat tcgtcgtaag ccgctggatg aaattggcgg cattgctgtc
     6181 gaaaccgtga ctgaagatgc gcatacttct ctgcgtttgc accgtcgtgg ctatacctcc
     6241 gcgtatatgc gtattccgca ggcggcgggg ctggcgaccg aaagtctgtc ggcgcatatc
     6301 ggtcagcgta ttcgctgggc gcgcgggatg gtataaatct tccgtctcga taacccgctc
     6361 accggtaaag gactgaagtt tgctcagcgg ctgtgctacg tcaacgccat gttccacttc
     6421 ttgtcgggca ttccacggct gatcttcctg actgcgccgc tggcgttcct gctgcttcat
     6481 gcctacatca tctatgcgcc agcgttgatg atcgccctat tcgtgctgcc gcatatgatc
     6541 catgccagcc tgaccaactc gaagatccag ggcaaatatc gccactcttt ctggagtgaa
     6601 atctacgaaa cggtgctggc gtggtatatc gcaccaccga cgctggtggc gctgattaac
     6661 ccgcacaaag gcaaatttaa cgtcaccgcc aaaggtggac tggtggaaga agagtacgtc
     6721 gactgggtga tctcgcggcc ctacatcttc cttgttctac tcaacctggt gggcgttgcg
     6781 gtaggcatct ggcgctactt ctatggcccg ccaaccgaga tgctcaccgt ggtcgtcagt
     6841 atggtgtggg tgttctacaa cctgattgtt cttggcggcg cagttgcggt atcggtagaa
     6901 agcaaacagg tacgccgatc gaaccgcgtg gagatgacga tgcccgcggc aattgcccgc
     6961 gaagatggtc acctcttctc gtgtaccgtt caggatttct ccgacggtgg tttggggatc
     7021 aagatcaacg gtcaggcgca gattctggaa gggcagaaag tgaatctgtt gcttaaacgc
     7081 ggtcagcagg aatacgtctt cccgacccag gtggcgcgcg tgatgggtaa tgaagttggg
     7141 ctgaaattaa tgccgctcac cacccagcaa catatcgatt ttgtgcagtg tacgtttgcc
     7201 cgtgcggata catgggcgct ctggcaggac agctacccgg aagataagcc gctggaaagt
     7261 ctgctggata ttctgaagct cggcttccgt ggctaccgcc atctggcgga gtttgcgcct
     7321 tcttcggtga agggcatatt ccgtgtgctg acttctctgg tttcctgggt tgtatcgttt
     7381 attccgcgcc gcccggagcg gagcgaaacg gcacaaccat cggatcaggc tttggctcaa
     7441 caatgatgat aacgcgatga aaagaaaact attctggatt tgtgcagtgg ctatggggat
     7501 gagtgcgttc ccctctttca tgacgcaggc gacgccagca acgcaaccac tgatcaatgc
     7561 tgagccagct gtagccgccc agacggaaca aaatccgcag gtggggcaag tgatgtcagg
     7621 cgagcaggga gctgatgccc ccatcgtggc gcagaacggt ccttcgcgtg atgtgaagct
     7681 gacctttgcg caaatcgcgc cgccgccggg cagcatggtg ctacgtggta ttaacccgaa
     7741 cggcagcatt gagtttggta tgcgcagcga tgaagtggtg acgaaggcga tgctcaactt
     7801 cgaatacacg ccatcgccat cgttactgcc tgtccagtcg cagttaaagg tttatctcaa
     7861 tgatgagctg atgggcgtgc tgccagtgac caaagaacag ttgggtaaaa aaacgctggc
     7921 gcaaatgccc attaatccac tgtttattac cgacttcaac cgtgtgcggc tggagtttgt
     7981 cggccattat caggacgtgt gcgaaaaccc ggccagcacc acgctttggc tggatgttgg
     8041 gcggagcagt ggactggatc tgacctatca gaccctgaat gtgaagaatg acctgtcaca
     8101 cttcccggtg ccattctttg acccgcgcga taaccgtact aacaccttgc cgatggtctt
     8161 tgcgggtgcg ccggatgttg ggctgcaaca agcatctgcc attgtcgcct cgtggtttgg
     8221 ttcgcgttct ggctggcgtg ggcagaactt cccggtgctc tataaccaat tgccggatcg
     8281 caatgccatt gtctttgcaa ccaacgacaa acggccggac ttcctgcgcg atcatccggc
     8341 ggtaaaagcc ccggtgattg agatgattaa ccatccgcag aatccttacg tcaaactgct
     8401 ggtggtgttt ggtcgtgacg acaaagacct gttgcaggca gcgaaaggta tcgctcaggg
     8461 taacattctg ttccgtggtg aaagcgtggt agtgaatgaa gtgaaaccgc tgctaccgcg
     8521 taagccgtac gatgcgccga actgggtacg taccgatcgt ccggtcacat ttggcgaact
     8581 gaaaacctat gaagaacagt tacaatccag cggccttgag ccagcagcga ttaacgtttc
     8641 gctaaacctg ccgccggatc tctacctgat gcgcagtacc ggcattgata tggatattaa
     8701 ttaccgctac accatgccgc cggtgaaaga cagttcgcgg atggatatca gcctgaataa
     8761 ccagttcctg caatccttca acctgagcag caaacaggag gcgaaccgtc tgctgctgcg
     8821 tattccggta ttacaaggtt tgctggatgg caaaacagat gtctctattc cggcgctgaa
     8881 actgggcgcg accaaccagc tgcgcttcga ctttgagtat atgaacccga tgccaggcgg
     8941 ttcggtagat aactgtatta ccttccagcc ggtgcagaat catgtggtga ttggtgacga
     9001 ttccaccatc gacttctcga agtattacca cttcatcccg atgccggatc tacgcgcctt
     9061 tgctaacgcg ggcttcccat tcagccggat ggcggatctg tcgcaaacca tcaccgtgat
     9121 gccgaaagcg cctaacgaag cacagatgga aacgttgctg aatactgttg gttttatcgg
     9181 cgcacagacg ggcttcccgg cgattaatct gacggtgacc gatgatggca gcaccattca
     9241 gggcaaagat gccgacatca tgatcatcgg tggtatcccg gacaaactga aagacgataa
     9301 gcagatcgac ctattggtgc aggcgaccga aagctgggtg aaaacaccga tgcgccagac
     9361 cccgttcccc ggcattgtgc cggacgagag cgatcgcgcg gcagaaaccc agtcaacgct
     9421 gacctcttcc ggtgcgatgg cggcggtgat tggcttccag tcgccgtata acgaccagcg
     9481 cagcgtgatt gcgctgttgg cagatagccc acgcggttat gaaatgctta acgatgcggt
     9541 gaacgatagc ggcaaacgcg ccaccatgtt cggttcggtc gcggtgatcc gcgagtccgg
     9601 tatcaacagc ctacgtgttg gcgacgttta ttacgtaggt catctgccgt ggttcgagcg
     9661 cttgtggtat gcgctggcaa accatccgat tctgctggcg gtgctggcga ctatcagtgt
     9721 gatattgctg gcatgggtac tgtggcgtct gctgcgaatt attagtcgtc gtcgtcttaa
     9781 cccggataac gagtaattga agatgaatgt gttgcgtagt ggaatcgtga cgatgctgtt
     9841 gttggctgcc tttagtgttc aggcagcctg tacctggcct gcctgggagc agtttaaaaa
     9901 ggattacatc agtcaggaag ggcgcgtcat cgaccccagc gacgcgcgca aaatcaccac
     9961 ctccgaaggg caaagttacg gtatgttctt tgccctggcg gctaacgacc gtgcagcttt
    10021 cgataatatt ctcgactgga cgcagaacaa tctcgctcag ggttctttaa aagaacgttt
    10081 gcccgcctgg ctgtggggca agaaagagaa cagtaagtgg gaagtgctgg acagcaattc
    10141 ggcctccgat ggtgatgtct ggatggcctg gtcgttgctg gaggcggggc gtttgtggaa
    10201 agagcagcgt tataccgaca tcggcagcgc gttgctaaaa cgtatcgcgc gggaggaagt
    10261 ggtgacggtg cctgggctgg gttccatgtt gttaccgggc aaagtgggtt ttgctgagga
    10321 taacagctgg cgttttaacc ccagctacat gccgccgacg ctggcgcagt atttcacccg
    10381 ctttggcgcg ccgtggacca cgctgcgcga aaccaatcaa catttattgc tggaaaccgc
    10441 cccgaaaggc ttttcgccag actgggtgcg ctatgagaaa gacaaaggct ggcagctaaa
    10501 agccgaaaaa acattgatca gcagctacga cgctatccgc gtttacatgt gggtaggcat
    10561 gatgcctgac agcgatccgc aaaaagcgcg gatgctcaac cggtttaaac cgatggcgac
    10621 attcactgag aaaaacggtt atccgccgga aaaagtggat gtggctacgg ggaaagcgca
    10681 gggtaaagga ccggtcggtt tttctgccgc catgctgcct tttttacaaa accgcgatgc
    10741 gcaggcagtt cagcgccagc gcgtggccga taactttccc ggcagcgata cctattacaa
    10801 ctatgtgctg accctgtttg gacaaggctg ggatcaacac cgtttccgct tctcgacaaa
    10861 aggtgagtta ttacctgact ggggccagga atgcgcaaat tcacactaaa catattcacg
    10921 ctttccctcg gtctggccgt catgccgatg gtcgaggcag caccaaccat cagcagaccc
    10981 tgcggatgga cagcggcaac actaatgccg tgcgcgggct ggcgaatatt taccgccagc
    11041 agtcgccaga aaaagctgaa gcgtttatcg cctcgctctc tgccagccag cggcgcagca
    11101 ttgatgatat cgaacgcagc ctgcaaaacg accgtctggc acagcaggca gaggtactgg
    11161 aaaaccaggg caaatgggcg caggcggcag cacttcagcg gcaacgactg gcgctgggcc
    11221 ccggcagcgt atggattact taccgactgg caggaagaag ctgcgcggca atccaacgat
    11281 ggcggcagca gtcagggctt cggctacacg gcgcgggcat tacttgaacg acgtgttact
    11341 tccaactggt ttgttggcac ggcaattgat atccagcagg tgaaagatta cgcacccagc
    11401 catttcctgc tctacgtacg ttattccgcc gccggatggc agggtgacat ggatttaccg
    11461 ccgcagccgc tgatacctta cgccgactgg taagttttca gatagcgcct ctcttaatgc
    11521 cgctgcgatc gggtatactc gggcggcaat ctgggatttc cggggggaga caatttgcgc
    11581 gtaagtcgct cgttaacaat caagcagatg gcaatggtgg cagccgttgt cctggtgttc
    11641 gtttttattt tttgcaccgt tttgctgttc catctggtcc agcagaatcg ctataacacg
    11701 gctacgcaac tggaaagcat tgctcgctct gtccgcgaac ccttatcttc agctattttg
    11761 aaaggcgata ttcccgaagc ggaagctatt cttgccagca ttaaaccggc aggcgtggtc
    11821 agccgtgccg atgtagtgct gcctaaccag ttccaggcgc tgcgtaaaag ttttattcca
    11881 gagcgtccgg tgccggtaat ggttactcgc ctgtttgagc taccggttca aatctcgctg
    11941 ggcgtctact cgctcgaacg tccggcaaat ccgcagccaa tagcctatct ggtgctacag
    12001 gcggattcct tccgtatgta taagttcgtg atgagcactc tctcaacgtt agtgaccatt
    12061 tacttacttt tgtcgcttat cctgaccgtc gccatcagct ggtgcattaa ccgcctgatt
    12121 ttgcatccgt tacgcaatat tgctcgcgaa cttaacgcca tcccagccca ggagcttgtt
    12181 ggtcaccaac tggcattacc gcgtctgcat caggacgatg aaatcggtat gttggtgcgc
    12241 agttacaacc tcaaccagca attgctgcag cgccattatg aagaacagaa cgaaaatgcg
    12301 atgcgcttcc cggtgtcgga tttgccgaac aaagccttgc tgatggagat gctggagcag
    12361 gttgtcgcgc gtaaacaaac caccgcgctg atgatcatca cctgtgaaac cctgcgtgat
    12421 actgcgggcg tgctgaaaga ggcgcaacga gaaattctgc tgctgacgct ggtggaaaaa
    12481 ctcaaatcgg tactgtcgcc acgtatgatc ctcgcgcaga ttagcggtta tgactttgct
    12541 gtcattgcca acggtgtaca ggaaccgtgg cacgcaatca ctttgggtca gcaagtactc
    12601 actatcatga gcgagcgcct gccgattgaa cgtattcaac tccgtccgca ctgtagcatt
    12661 ggcgtggcga tgttctacgg cgatctcacc gccgaacagc tttacagtcg cgctatttct
    12721 gcggcattta ccgctcgcca taaaggcaag aatcagattc agttctttga tccgcagcag
    12781 atggaagcag cccagaagcg gttgacggaa gagagcgata tccttaatgc actggaaaat
    12841 catcagtttg caatttggtt acagccacag gtcgagatga ccagcggtaa actggtcagt
    12901 gcggaagtgt tactgcgtat ccagcaaccg gatggcagtt gggacctgcc ggatggctta
    12961 atcgatcgca ttgagtgctg tgggctgatg gttaccgtcg gtcactgggt gctggaagag
    13021 tcctgtcgat tgcttgcagc ctggcaagag cgcggcatta tgctgccctt gtcggtaaac
    13081 ctctctgcgc tgcaactgat gcacccgaat atggtggcgg atatgctgga actgttaacc
    13141 cgctatcgca ttcagccggg aacactgatt ctggaagtga cagaaagccg acgtattgac
    13201 gaccctcatg ctgcggtggc aatcctccgt ccgctgcgca atgccggagt tcgggtggcg
    13261 ctggatgatt tcggcatggg ctacgcaggg ctgcgtcagc tgcagcatat gaaatcgttg
    13321 ccaatcgacg tactgaaaat cgacaaaatg tttgttgaag gcttgccgga agatagcagc
    13381 atgattgctg caattatcat gctggcgcag agcctgaact tacaaatgat tgccgaaggc
    13441 gtggagactg aagcacaacg agactggctg gcaaaagcgg gcgttggtat tgcccagggc
    13501 ttcctttttg ctcgcccact ccctattgaa atcttcgaag agagttacct ggaagaaaag
    13561 tagctacccc aaactgatta caaaacttta aaaagtgctg gtttgtgcga gccagctcaa
    13621 actttttaac ctttttgttt caattatgat ccaggtacat ttctgtgatg ttgtctgggt
    13681 gttattttaa ggccgcaggt accccccccc ccataacctt acaagacctg tggttttact
    13741 aaaggacacc ctatgaaaac ctctctgttt aaaagccttt actttcaggt cctgacagcg
    13801 atagccattg gtattctcct tggccatttc tatcctgaaa taggcgagca aatgaaaccg
    13861 cttggcgacg gcttcgttaa gctcattaag atgatcatcg ctcctgtcat cttttgtacc
    13921 gtcgtaacgg gcattgcggg catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc
    13981 gcactgcttt actttgaaat tgtcagtacc atcgcgctga ttattggtct tatcatcgtt
    14041 aacgtcgtgc agcctggtgc cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg
    14101 gtagcggttt acgccgatca ggcgaaagac cagggcattg tcgccttcat tatggatgtc
    14161 atcccggcga gcgtcattgg cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt
    14221 gccgtactgt ttggttttgc gctccaccgt ctgggcagca aaggccaact gatttttaac
    14281 gtgatcgaaa gtttctcgca ggtcatcttc ggcatcatca atatgatcat gcgtctggca
    14341 cctattggtg cgctcggggc aatggcgttt accatcggta aatacggcgt cggcacactg
    14401 gtgcaactgg ggcagctgat tatctgtttc tacattacct gtatcctgtt tgtggtgctg
    14461 gtattgggtt caatcgctaa agcgactggt ttcagtatct tcaaatttat ccgctacatc
    14521 cgtgaagaac tgctgattgt actggggact tcatcttccg agtcggcgct gccgcgtatg
    14581 ctcgacaaga tggagaaact cggctgccgt aaatcggtgg tggggctggt catcccgaca
    14641 ggctactcgt ttaaccttga tggcacatcg atatacctga caatggcggc ggtgtttatc
    14701 gcccaggcca ctaacagtca gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg
    14761 ctgctttctt ctaaaggggc ggcaggggta acgggtagtg gctttatcgt gctggcggcg
    14821 acgctctctg cggtgggcca tttgccggta gcgggtctgg cgctgatcct cggtatcgac
    14881 cgctttatgt cagaagctcg tgcgctaact aacctggtcg gtaacggcgt agcgaccatt
    14941 gtcgttgcta agtgggtgaa agaactggac cacaaaaaac tggacgatgt gctgaataat
    15001 cgtgcgccgg atggcaaaac gcacgaatta tcctcttaat ctcaccactt atgcccgcac
    15061 tcccttcccc gagtgcgggt gtttgcgcat aattgacccc tgtgcctgaa cttaccacct
    15121 gggtgagttc aggtttattt cacttcttcc gtgacatttt catgttcttg cggtctaaca
    15181 cgaagtgttt ttaacgtcat ttagactgac cgacaacggg cagttttttt attaccagga
    15241 tagttgatca ggggttcaca tgcagggcat aaaaattcga cttttagcgg gcggtttgct
    15301 gatgatggcc actgctggct atgtgcaggc agatgcgctc cagcctgatc cagcatggca
    15361 acaggggacg ctttccaacg gtttacagtg gcaagtgctg accacccccc agcgtcccag
    15421 cgatcgtgtt gaaattcgcc tgctggttaa taccggttcg ctcgccgaaa gtacacaaca
    15481 gagcggttac agtcacgcca tccctcgtat tgcgctaacg caaagcggtg gccttgacgc
    15541 agcacaggcg cgttcattgt ggcagcaggg gatcgaccct aaacgcccga tgccgccggt
    15601 aattgtctct tatgacacca cgctgtttaa tctgagtttg cccaataacc gtaacgattt
    15661 gctgaaagaa gcgctctctt atctggcaaa tgccactggc aaattgacca tcacaccaga
    15721 aaccatcaac cacgcgctgc aaagtcagga catggtggca acctggcctg ccgatactaa
    15781 agagggctgg tggcgctatc gtctgaaagg gtcaaccttg ttaggtcacg atcctgccga
    15841 tccgctgaaa caacccgttg aagcggaaaa aattaaagat ttctatcaga aatggtacac
    15901 cccggatgca atgacgctac tggtggtggg aaacgtggat gcgcgctcgg ttgtcgacca
    15961 aatcaacaaa acgtttggcg aactgaaagg caaacgtgaa acgccagctc cggtgccgac
    16021 gctttctccg ctgcgtgcgg aagcggtgag tattatgact gacgcggtgc gtcaggaccg
    16081 gttatctatc atgtgggata cgccgtggca gccgattcgt gaatcagccg cactgctgcg
    16141 ctactggcgt gcggacctgg cccgcgaggc gctgttctgg catgttcagc aagcgttaag
    16201 cgccagtgac agcaaagaca tcggtcttgg atttgactgc cgtgtgctgt atctgcgtgc
    16261 gcagtgtgcc atcaacatcg aatcaccaaa cgacaagctg aacagcaacc ttaatctggt
    16321 ggcgcgtgaa ctggcgaagg ttcgcgataa aggtctgccg gaagaagagt tcaatgcgtt
    16381 agtggcgcaa aagaaactgg agctgcagaa actgtttgcc gcctatgcac gggctgatac
    16441 cgatattctg atgggccagc ggatgcgttc gttgcaaaat caggtcgtcg atatcgcgcc
    16501 ggagcagtat cagaaactgc ggcaggattt ccttaacagc ctgactgtgg agatgttaaa
    16561 tcaggatctg cgtcagcagt tgtcgaatga tatggcgtta atactgctgc agccgaaagg
    16621 cgagccggaa tttaacatga aagcgttgca ggcggcctgg gatcaaatca tggccccatc
    16681 taccgccgct gcgaccacct ctgtcgccac ggatgacgta catcctgaag tgacggatat
    16741 tccacctgca cagtaatgcc tgatggtgat gagttagcac aaccatcagg tatttatgga
    16801 tgagctggat agaggggtaa cactttatcc agccttttgc atatgctgcg tttacgctgg
    16861 catcgcctca cgcgggataa tcgcgccgcg atactgaata acggtacttg cggtcaggtg
    16921 cccgcgtttc gccgcgtttt ccgcactgcc gcccgtcaaa cgtaccgcca gataaccggc
    16981 gctgaaagag tcgccagctg cggtggtatc gatcactttt tctttcggca gtttcaccgc
    17041 cggaacatca actaacgctt caccagcaat ggacaccagg caagaatccg ccccgcgttt
    17101 caccaccact tctttcacgc ccgcgttatg ggtgcgcgca atgacgtctt ccaccggctg
    17161 ttgaccccac agcgcgtctt cgtcgtccag cgtcaggaag gcgatatccg tgcattccag
    17221 catttgctgg tacacctgct gtgtctcttc tttgctggcc cacaggcgcg gacgatagtt
    17281 attgtcgaaa atcacttttc cgcctttggc gcggcattcg cgcagcaggg aaagcagctt
    17341 ttcgcggctg gtcgggctta agatcgccag gctaatcccg ctcaggtaga gataatcgaa
    17401 attcgccagc tcttcgcaaa tcgccgcaga ctgctcgctc gccagccaga atttggcggc
    17461 ggcttcgtta cgccagtagt agaacgtacg ttcgccggtg ctgtcggttt caatgtagta
    17521 aaggcccgga agacggtttt ccatccgttg ggtcagggaa gtatcaacgt tctcgccgtg
    17581 ccaggcgtcc agcatctgct ggctaaaact gtccgttccc agcgccgtta cgtaatgaac
    17641 ggttaatgcc gcaggatcga cctgacgggc gatatagacg gaagtgttca gggtatcgcc
    17701 gccgaaaccg cgcttaacgt ccgcgccttt ctcggaaagc tcaatcatgc attcgccaat
    17761 cacggcaatc tttttggaca tagtcgtgaa cctgatctgt aaaaaattag cgttagtttg
    17821 cgctgtggtg gtttgctggt caaccatatt aaaacagtgt tccattattt ttttgagcca
    17881 gaacgtattc ctgaaagatt ccgttgtgga gtgaggaaat ttaccccggc caggcggtaa
    17941 agttctgccc ttacgcgccg ataatctttg tcgagtccgg gcagcatcac ttttaaacac
    18001 aggacatctt tgatgataag gcaggttatc cagcgaataa gcaaccctga agcaagcatc
    18061 gagagcttgc aggaacggcg tttttggttg cagtgtgagc gtgcttacac ctggcagccg
    18121 atctatcaaa catgcgggcg gttaatggcc gtggagctat taacggtggt cacgcatccc
    18181 ttgaaccctt cgcaacgcct gccgccggat cgctatttta ctgaaatcac cgtcagccat
    18241 cggatggagg ttgtgaaaga gcagattgat ttgctggcgc aaaaagccga cttctttgta
    18301 gagcacggcc tgctggcatc ggtcaatatt gatggcccta cgctcatcgc cctgcgtcag
    18361 caaccaaaaa tcctgcgcca gattgagcgt cttccctggc tgcgtttcga actggtggag
    18421 catatccgtc tgccgaaaga ttcaaccttt gcctcgatgt gtgaatttgg cccgctgtgg
    18481 ctggatgatt ttggtaccgg gatggcaaat ttctctgcac taagtgaagt gcgttatgac
    18541 tacatcaaaa tcgcgcgaga actgtttgtg atgctgcgtc agtcgccgga aggccgcaca
    18601 ctcttttctc agcttttaca tctaatgaat cgctattgtc gcggggtgat tgtcgagggc
    18661 gtagaaacac cggaagagtg gcgtgatgtt cagaactcgc ccgcattcgc cgcacaaggc
    18721 tggtttcttt cacgcccggc accgatggaa acgctgaata cggcggttct ggcgctataa
    18781 gctgcctcat tttccgccgg gctggactat ctttaggact ggcacaggaa gaatatgagg
    18841 aagcgaacga tgagcaaggc aggcaaaata accgctgcga tttcaggggc tttcttgttg
    18901 ttgattgtcg tggcgatcat tttgattgca acttttgact ggaatcgact caaaccgacc
    18961 atcaaccaga aagtctctgc ggagttgaat cgtccgttcg ctatccgtgg caatctgggc
    19021 gtggtgtggg agcggcaaaa acaagaaact ggctggcgca gctgggtgcc gtggcctcat
    19081 gtacatgcag aagacatcat tcttggtaat ccaccggata ttcccgaagt cacgatggtg
    19141 catttgccgc gagtagaggc aacgctggca ccgctggcgc tgctgaccaa aacggtctgg
    19201 ctgccgtgga tcaagctcga aaagcccgac gcgcgcctga ttcgcctctc tgaaaagaac
    19261 aataactgga cgtttaatct tgccaacgat gacaacaaag acgcgaatgc aaagccgtcg
    19321 gcatggtcgt ttcggctgga taatattctt ttcgatcaag ggcggatcgc cattgatgac
    19381 aaagtaagca aagcggatct ggaaattttt gttgatccgt taggcaagcc gctgccattc
    19441 agcgaagtta ctggatcgaa aggtaaagcg gataaagaaa aggtgggcga ttacattttt
    19501 ggcctgaagg cgcagggacg ttataacggt gaaccgctca cgggtacggg aaaaataggc
    19561 ggtatgctgg cgctgcgtgg cgaagggacg ccgtttccgg tacaggctga tttccgttca
    19621 ggtaataccc gtgttgcttt tgatggcgtc gtgaatgacc caatgaagat gggcggtgtc
    19681 gatttacggc ttaaattttc tggcgattca ctgggtgatc tctatgaact gacgggcgtt
    19741 ctgctgcccg ataccccgcc gtttgaaacg gatggtcggc tggtagcgaa aatcgacact
    19801 gaaaaatcgt cggtctttga ttatcgcggt tttaatgggc gaattggcga tagcgatatc
    19861 cacggttctc tggtctacac caccggaaag ccacgaccaa aactggaagg tgatgtcgag
    19921 tcgcggcaat tgcggctggc ggacctggga ccgttgattg gcgttgattc cgggaaaggg
    19981 gcagaaaagt cgaaacggtc tgaacagaag aagggcgaaa aaagcgttca gcctgcgggc
    20041 aaagtgctgc cttatgaccg cttcgaaacc gataaatggg acgttatgga tgccgatgtt
    20101 cgcttcaaag ggcggcgcat tgagcatggc agtagcctgc cgattagcga tctttctact
    20161 catatcatcc tcaaaaatgc tgacctgcgc ctgcaaccgc tgaaatttgg catggcgggc
    20221 ggcagcattg cggcgaatat tcatctggaa ggcgataaaa agccgatgca ggggcgggca
    20281 gatattcagg ctcgtcgact gaaactgaaa gaactgatgc ccgatgtgga actgatgcag
    20341 aagacgctgg gggaaatgaa cggtgacgcg gaactacgcg gtagcggtaa ctcggtggcg
    20401 gcgcttttag gcaacagtaa cggcaacctg aaactgttga tgaatgacgg gctggtgagc
    20461 cgcaacctga tggagattgt tgggctgaat gtcggcaact acattgtcgg tgcgatattt
    20521 ggtgacgatg aggtgcgggt gaactgcgcg gcggcgaatc tgaatattgc caacggcgtg
    20581 gcgcgcccgc agatttttgc tttcgatact gagaacgcgt tgattaatgt taccggcacg
    20641 gcaagttttg cttcggaaca gctggatttg actattgatc cggagagtaa agggattcgg
    20701 attatcacac tgcgttcgcc gctgtatgtg cgtgggacgt ttaaaaatcc gcaggctggg
    20761 gtgaaagccg gaccgctgat tgcccgtggt gctgttgctg cggcactggc aacgctggta
    20821 acaccggcgg cggcgttact ggcactgatc tcaccttccg aaggggaggc taatcagtgt
    20881 cggacgatat tgtcgcagat gaagaagtga tgataattgc cggatgtctg acatccggca
    20941 attaaacaga tttagatttt tggcaaagtg tgctttgttc atgccggatg cggcgtgaac
    21001 gccttatccg gcctacaaat ccttgtgaat tcaatatatt gtatggaaat tcagtcctga
    21061 taagcgtagc gcatcaggct tttcacactt attacaacga ctgatgtcgc gtctcatggg
    21121 tcagcagcag ggcgattaac gtcaagccag ccatcgccgc cagatataac cccaccgcac
    21181 ctaacccgta gttagcctgc aaccaggctg cgatatacgg cgcaacggaa gccccgagaa
    21241 tcgacgatac gttgtaagag aacgatgctc cggtgtaacg cacttctgtc ggaaacagct
    21301 ctggtaacag cgcacccatc ggcccgaagg tcagccccat cagacttaac cccagcagca
    21361 ggaaggcaaa aaccagaatc gggttgccag aaccgagcag cgggttaaag gcgaacagcg
    21421 cgaacaggat gatcagcgtg gtgatgatca ccatgctttt acggcgacca aaggcatcag
    21481 ccagtaatcc ggcgactggc accatcacgc caaaaccaat aactgccatc atcagcatcc
    21541 acaacacttc gttacgcggc aggccaagcc caactggcgc ggcggcggta ctgaaggtca
    21601 tagagtagac cgtcatgatg taaaacagcg tataggttgc cagcataatg aacgtaccca
    21661 gtacggttac gcgaacatgt ttggtcagca gcgtacccag cgggatcttc acctgttttt
    21721 tcgctttagc gactttctca aacaccggcg actcatgcag cgacacgcga acatacaggc
    21781 cgataatgac cagcaccgcc gagaagataa atggcacacg ccagccccag ctcataaact
    21841 gctcatcggt cagtagccag gaaagcagca agaaagtgcc gttggcaaag aagaagccaa
    21901 tcggtgcgcc tagctgtggg aaggagccat acagtgcacg tttgcgcggt ggggcgtttt
    21961 cagtcgccag cagcgccgcg ccgccccatt caccgcctaa gcccagaccc tgaccaaatc
    22021 gagccagcgc cagcagcagt ggggcgaaaa taccaatcgt ggcatagccc ggcagcagac
    22081 caatcaccac ggtcgaaatc cccatcgtta gcaacgaggc gaccagcgtc gctttacgcc
    22141 caacgcgatc gccaaaatga ccaaaaacgg cagagccaat ggggcgcgcg acgaaggcga
    22201 tggcgaaggt ggcgagcgac tgtagcgttg ctgccgttgg atcgccctgc gggaagaaga
    22261 tatgcggaaa cacaataacg gccgcagtgg cgtaaatgta aaagtcgaag aactcaatgg
    22321 ccgtgccaat gagagaggcg acaaggactt tattacgcga gttgatcggc gtgtattctt
    22381 gttcgtggtc gagtgttgtg gctgttgctt gcataatctt ttcttatttt ttggctaacg
    22441 aatagccata ttacgcacag caaaagtgat atttcaatgt gtaacaaatc gcggaaatgg
    22501 tcaaaaacca ggcaaaaagg ggataatgtt taggccagtg atttttcttc tatgaaatgc
    22561 ttcacataat tttataaata gtggataaaa ctggtttcag gttaaataaa agttaccaac
    22621 tggatttata tgctggggtt atgagttgga gtgaaattgt ttgctcccaa atagatggct
    22681 gaagaggctc tggggaagag tttcaagagg ttatgtgcgc attatagccg gatgcgatgc
    22741 tggcgcatct tatccggcct acggggagcc aggccgaata aaatcgtatg ccggataagg
    22801 cgtttacgcc gcatccggcc tattttaagg ctgcgttttc cctggcattc gcgggtcgtc
    22861 tttatattcg gcggtggcaa tccacgccgc gcaaaacagc gtcaaacggg cgaagaagta
    22921 gaaaaatgcc atcagcccca gcacggaacc aaatgctgcg ccagaggggg atttcatcag
    22981 cgatggcagg gtgtaggtca tcacgatttt aatcacttca aaaccaatag cagccagaaa
    23041 tgttccgcga atcagggctt ttttgcgcgg acggtggcgc ggcaaccgcc agaagatcca
    23101 gaagaaaagc agatagttgg cgaagatgga aatcgccagg ccaatcaatc gccacgtcgg
    23161 tttcagccac tcaatgctgt tcaggtgcag ggcgctaata atcatttgct gcgccgaacc
    23221 ggcaaccgag gtgatcgaaa gcgtcacaat cagcgcaatc aacaaaccaa tcagtgaaat
    23281 aaaatcacgc agatatttta cccagaactt ctcctgatct tgcggcgagc gttcccagac
    23341 atcgcgcgac tgggcacgaa tcgcttcacg caggttaccc atccagttga tgccggaata
    23401 aagtgccacc gccaggccga caagccctac agtcgtacgc tgctgaacgg cggtgttgat
    23461 ggtgtttttc aacgtggcgg ctagtgtcgg atcgctgatg ttttgcagaa ttttgtcgaa
    23521 gatatcctgt agcaacatcg gatgggaggc cagcacaaag ccccctgcgg caaacgacac
    23581 catcaaaatc gggatcatcg acaaaaatga gaaataggtg atagccgcac caaactggtt
    23641 acccagccga tcgttaaagc gttctgtcgc gcgaataagg tgcgcaataa ccggttggcg
    23701 ttggactttt tcggcagtgg tggtgacggt ttccagcgcc tggctaactt tgccgccttt
    23761 ctcgctatta tccaactgct taatcggctc gtgctccaga tcctgggtgg gacgtttgat
    23821 ctcgttttcc tgcgtcatcc gctcttttgt ccttctgtcg tggtcattct taaaaagtat
    23881 agtcggtcag tccaggtgct ctttcattac gccagccagc cactgcataa acaggtttac
    23941 acgccgggaa agctcccgac gctgcgggta aaccagcgaa agggagagcg gctcggcacg
    24001 gtagcctggc aacacttcaa taagccgccc ggcacgcagg gcttcgcgca cggcaatgcg
    24061 cgggatctga ataatcccca gaccggcaag accggcggcg agatagtttt cgctactgtt
    24121 taccgtcaac atgccgccag acttaaacca ctggacgcca ttaacgctgg caacctcaaa
    24181 acctaacgga tgtacaccca ggtgcggtgt ataacgcact attgcgtgtg aagtcagatc
    24241 atcggggctt tgcggataac caaagcgcgt cagatagtgc ggactggcac agttgaccat
    24301 ggttagtttg ccgaggggac gggcgataac gccatcttcc ggtaacgcgc cagtgcgtat
    24361 cacgcaatca aaaccatcat gaagaatatc taccggacgg ttatggctac tcagttccag
    24421 ctcaattccc ggatgctgat agagaaattc cgacagacgc ggcagtaaca ggtttttcgc
    24481 gattcctgac gggatgtcga tgcgtaattt accgctgata ctggtggcat cctgttgaaa
    24541 cagaccgtcc agttcgctga gattactcaa cacatctttt gcccgttgat aataggtcat
    24601 gccttctggc gtgagtttga cccgtcgcgt agtgcgctgg agaagctggg tgccaagttg
    24661 atgttccagt gcctgtatct ggcgcgaaac acttcccttt ggcaaagcaa agaaatccgc
    24721 cgcgcgggaa aaactttcca gctccgcgac tttgatgaac aactgcattg cgtgaatttt
    24781 atccatttct ttgaccgatt gttgttcaca gcgaaacagt aacgcgcata tcgcattgtt
    24841 tattgttttt tctaacatct aatgccgggt ttcacaatgt tgtcatgacg aatgtggcga
    24901 tttctcagca ggaaaagggc ggaatagctt tatgagaaag gtgaatcccc ttaaacagag
    24961 cgtatttttc agtgattccc tttaaagtga atagaggacg tattggctat ttgtgcttat
    25021 ttttgtctca ttgccatttg ttattttggc ttgtagtcgc ggaatttcat ttttaattgc
    25081 atgcgtacta accaccaata taaggaaatc ctcaaaattg ttttaaatag tgccctcgac
    25141 atgtaatttc aggcgtttag ctgtaactat ttatatcggt aactattttc tacgcgtcat
    25201 ctctgtagaa tggccaccgc caaatggcga attcttattt tttctcgcgg gtaataccct
    25261 tccactactt aagtcttttt aacgtattca ggtggagatt cagttatgca aatagtcatg
    25321 tttgacaggc agtcaatatt tattcatgga ataaaaatca gtttacagca gcgtattcca
    25381 ggagtgagta ttcagggggt cagtcaggca gacgagttat ggcaaaagct ggaaagttac
    25441 cctgaagcct tagttatgct cgatggcgat caggatggtg agttttgcta ctggttgctg
    25501 caaaaaaccg tggtgcagtt tcctgaggtt aaggtgttaa tcacggcgac ggattgcaat
    25561 aaacggtggt tacaggaagt tatccatttt aatgtgctgg ccattgtgcc tcgcgattca
    25621 accgtcgaga catttgcgtt ggcggtaaat agtgcggcga tggggatgat gtttctgcct
    25681 ggagactggc ggacgacgcc ggaaaaggac attaaagatc tcaaatcgtt aagcgcccgc
    25741 cagcgggaga ttttaaccat gttagcggcg ggcgaatcaa ataaggagat cggcagagcg
    25801 ctgaatatca gtaccggaac ggtaaaagcg catctggaat ccctgtatcg ccgtctggaa
    25861 gtgaaaaatc gcacccaggc ggcaatgatg ttaaata
//
LOCUS       DAJVQR010000055        25427 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVQR010000055 DAJVQR010000000
VERSION     DAJVQR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            537..1559
                     /locus_tag="OQM52_002399"
     CDS             537..1559
                     /locus_tag="OQM52_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCS1564216.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1559..2539
                     /locus_tag="OQM52_002400"
     CDS             1559..2539
                     /locus_tag="OQM52_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCS1564217.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2536..3294
                     /locus_tag="OQM52_002401"
     CDS             2536..3294
                     /locus_tag="OQM52_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1564218.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3304..4113
                     /locus_tag="OQM52_002402"
     CDS             3304..4113
                     /locus_tag="OQM52_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCS1564219.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4113..4967
                     /locus_tag="OQM52_002403"
     CDS             4113..4967
                     /locus_tag="OQM52_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCS1564220.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            4993..5550
                     /locus_tag="OQM52_002404"
     CDS             4993..5550
                     /locus_tag="OQM52_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCS1564221.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            5487..6947
                     /locus_tag="OQM52_002405"
     CDS             5487..6947
                     /locus_tag="OQM52_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1564222.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6997..7251)
                     /locus_tag="OQM52_002406"
     CDS             complement(6997..7251)
                     /locus_tag="OQM52_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1564223.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7452..8186
                     /locus_tag="OQM52_002407"
     CDS             7452..8186
                     /locus_tag="OQM52_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1564224.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8188..8799)
                     /gene="emtA"
                     /locus_tag="OQM52_002408"
     CDS             complement(8188..8799)
                     /gene="emtA"
                     /locus_tag="OQM52_002408"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1564225.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8899..9813
                     /gene="ldcA"
                     /locus_tag="OQM52_002409"
     CDS             8899..9813
                     /gene="ldcA"
                     /locus_tag="OQM52_002409"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1564226.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            9908..11644
                     /locus_tag="OQM52_002410"
     CDS             9908..11644
                     /locus_tag="OQM52_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1564227.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            12255..13669
                     /locus_tag="OQM52_002411"
                     /pseudo
     CDS             12255..13669
                     /locus_tag="OQM52_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13837..14907)
                     /gene="dadX"
                     /locus_tag="OQM52_002412"
     CDS             complement(13837..14907)
                     /gene="dadX"
                     /locus_tag="OQM52_002412"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS1564228.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14917..16215)
                     /gene="dadA"
                     /locus_tag="OQM52_002413"
     CDS             complement(14917..16215)
                     /gene="dadA"
                     /locus_tag="OQM52_002413"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1564229.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16545..18077
                     /locus_tag="OQM52_002414"
     CDS             16545..18077
                     /locus_tag="OQM52_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1564230.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(18129..18848)
                     /gene="fadR"
                     /locus_tag="OQM52_002415"
     CDS             complement(18129..18848)
                     /gene="fadR"
                     /locus_tag="OQM52_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1564231.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            19069..20610
                     /gene="nhaB"
                     /locus_tag="OQM52_002416"
     CDS             19069..20610
                     /gene="nhaB"
                     /locus_tag="OQM52_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1564232.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20756..21286
                     /gene="dsbB"
                     /locus_tag="OQM52_002417"
     CDS             20756..21286
                     /gene="dsbB"
                     /locus_tag="OQM52_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1564233.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(21332..22600)
                     /gene="umuC"
                     /locus_tag="OQM52_002418"
     CDS             complement(21332..22600)
                     /gene="umuC"
                     /locus_tag="OQM52_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1564234.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22600..23019)
                     /locus_tag="OQM52_002419"
     CDS             complement(22600..23019)
                     /locus_tag="OQM52_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1564235.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            23392..24292
                     /gene="hlyE"
                     /locus_tag="OQM52_002420"
                     /pseudo
     CDS             23392..24292
                     /gene="hlyE"
                     /locus_tag="OQM52_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(24499..24945)
                     /locus_tag="OQM52_002421"
     CDS             complement(24499..24945)
                     /locus_tag="OQM52_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1564236.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(25037..>25427)
                     /locus_tag="OQM52_002422"
     CDS             complement(25037..>25427)
                     /locus_tag="OQM52_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1564237.1"
                     /translation="KAIAGYGVALDLTLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFI
                     PAAEFTGDPQNTTLGLSVNGEKRQQGTTADMIHKIVPLIAYMSKFFTLKAGDVVLTGT
                     PDGVGPLQSGDELTVTFDGHSLTTRVL"
BASE COUNT         6266 a         6563 c         6241 g         6357 t
ORIGIN      
        1 gaataaacgc attcagtaaa ttactctctc taagaaaata aatatcttca ttgttgttaa
       61 tgacattaat aacgtcgttc ttaaaatcct caaagtcttg ttgcggtaac ttctggttac
      121 gctgcttact atcagcaagc ggtgaatccg gacgacaaca acataggttt tgtataaccc
      181 ccagcattct tttcacctcc gttaacagta tattcacgac actgaaacga gagaatgctg
      241 ataattttct cggcgagata tcaaaaatcg ctcattataa aacaagtaac ttcaacgtcg
      301 cgtaaccgtg gacgataaaa aaacaataat gtcattcata tttaaataat ttagccttaa
      361 atgatttaaa taatattagc cacctgataa atacattttt aatgatagag agggtcaaca
      421 ggcttgcgga agtcgtcaca tcttcatgtt ttcaatagct aaactttgat attgaacatg
      481 atttttatcg atatatatga aagtatatat cgattttact tttcaggatg ttcaccatgc
      541 ccataaccag acggacgttt gcgcaggcac ttgcatctac cctgctcctg cagtctctac
      601 cgtccttttc acaaacagtt aatcgttttg cttcgcaatc tcttcccgaa gcccaaaaca
      661 ttacacgcat cgtcagcgca ggcgcgcccg ccgatttgct cttgctggct gtcgcgccag
      721 aaaaaatggt cggtttttcc tcttttgatt ttgcccgtca ggcattaatt ccattgccag
      781 agcacattcg ccagttaccc aggctgggac gactcgccgg gcgcgccagc acactatcgc
      841 tggaagggct tatggcgtta catcccgatt tggtcgttga ttgcggcaat acggatgaaa
      901 ccttgatctc ccaggcacgg caggttagcg aacagacaca aatcccctgg ttattgctta
      961 acggaaaact ggcgcaatca gcagaacagt taacaacgct tggcaaaacg ttaggcgaag
     1021 agcaccgcgc cgcagaacaa gccaatctcg ccagccactt cgttggtgaa gctcaggcat
     1081 tcgccacctc acccgccgct aacctccgct tttatgctgc gcgcggtccc cgagggctgg
     1141 aaacgggctt acagggttcg ttgcataccg aggcggcgga attattaggt ttgcacaacg
     1201 tcgcgcaaat agccgatcgc cacggcctga cacaggtttc catggaaaat ctcctgcgct
     1261 ggcagccgga tattattctg gttcaggagg ccgttactgc agattttatt cgtcgtgatc
     1321 ctctctggca gggcgtgaaa gccgttgcgg aacaacgcat cttattttta agtggcttgc
     1381 cctttggctg gctcgatgcc ccgccgggaa tcaaccgtct tctgggatta cgcagacttc
     1441 acgcctggct ggatcccgcc atcaatcgcc agtttaaaag tgacatgcag cattacgccc
     1501 aactgttctg gcattgttca ctcagtgatg ccgactatca aaaattggtg gcgagctaat
     1561 gagaatcgta aacggatgta ttttgctgct ggcagctatc agtatcactt ttgccgccgt
     1621 aagtggggct tatcatctcg atatgcagca actgctggcg ctaattcttc gtcaagaaaa
     1681 tgagtctgtg caggaacaaa ttgtcttctg gcaaatccgc gtgccgcgta ttcttgctgc
     1741 gctgttactg ggcgcagcgc tggcaggagc cggaaccacc tatcagggaa tgttgcgtaa
     1801 tcccttagtt tcaccggata ttttaggcgt ctccgccggt gccggacttg gtgcctgcgc
     1861 ggcaatcctg tggggattac ccattgttta catccagctg tacgcatttt gcggcgggct
     1921 gatggtggtc gcgggcgtct ggttgattac gcgtcgggtg acccgccacg atccgatctt
     1981 aaccctggtg ctggtaggca ttgcgttagg aacgttgtgc ggagcgggga tatcgctaat
     2041 taaaacgctg gccgatcctt acacccagtt accgtcaatt accttctggt tgctgggggg
     2101 actgtccacc gtgaccttac gcgatctctg ctatgctgca ccgatcattc tcataggctc
     2161 cttgcccctg ttttttctcc gctggcgcat gaatttactc acgctttccg acgacgaagc
     2221 ccgttcgctg ggactgaacg tgacgcgtct gcgctttgga ttgattgtct gcgccacact
     2281 aatcaccgcc agcacggtag caatagcggg cataattggt tggatcggtc tggtcgttcc
     2341 gcacattgcc cgcttgttga ccggtcataa ccatcagcag ctgttgccta tggcaatgtg
     2401 tactggcgct attctgctcc tgcttaccga tacgctggcg cgcagcattg gaacaacaga
     2461 aatcccactt ggcattctga ccgcttttgt tggcgcaccg tttttcctgc ttttgttgtt
     2521 acgcggaggt cgccagtgac tttgcttgcc gttcgtcatg cgtctctggg ttactcccgc
     2581 catccagtgc tacgcgacgt gtccttcacc ctgtcgcaag gaacaatttg ttgtctgctt
     2641 ggcgcgaatg gttgcggcaa aaccacactc atgcgctcca ttcttggcgt tataccgtta
     2701 ttaaaaggcg agatcctact ggatagcctc tctgtgcaga cactcagcca tcgccagcgc
     2761 gcacaggcta ttgcctgggt gccgcaggcg cacgacggta tttttgcttt cagtgtgctg
     2821 gatatggtgt tgatgggcct ggcccccacc ataggcgctt tttccgttcc cggtaagcaa
     2881 gaacgcctga aagccacgga gcaactggaa aagctgggca ttttgcatct ggcgacacga
     2941 cgctggaata cgcttagcgg cggtgaacgt cagctggctt taatcgcccg tgcactcgtc
     3001 caacaaccgc gcttgttgtt actggatgag cccgcctcca gcctcgattt cggtcatcag
     3061 atccaacttc tggacacgct ggcgcagctc aaaaataacg gcatgacaat gttaatgtcg
     3121 actcaccata cgctccatgc caacgccatt gccgacagca ttatccaagt agagccagat
     3181 ggacgagtaa cacaaggctt accgaccgaa cagttaacaa cgaacaaact ggcggccctg
     3241 tatcgggttt ctgccgacca aattcatcat cacctctctg caattagccg ctaaggaaca
     3301 atcatgctca ttgatgatat tgattttgca gacctttatc tgcagcaact caaactggct
     3361 caccgcacag aaaaaacgcc cgatcactgg gatcaacggg cggagaaaat ggcggaaaat
     3421 tgcgcctcgc caacggacag ctacctgcaa caactgatcg ctaaaattga cctgcaaggc
     3481 gctcagacct tattcgatat gggctgcgga ccaggcaccg tatcgctggc gctggctgac
     3541 aaactcacta cggtttatgg cgttgattac agtcagggga tgctgaacgt tgccgcccgc
     3601 cgcgccgccg cactgaaggc tgacaatgtc cactggatcc agcgcgcctg ggaagaggac
     3661 tggagtgacc tgccacgctg cgatattgcc gtggcttccc gctccactct ggtggctgat
     3721 atgcgccatg caatgagcaa actgaataat caggcacggc ttcgcgtcta tacaactcat
     3781 ctggtcagca cctcgttcgt ctctccagcc attcagcggg cggctggtcg ggaagtgatc
     3841 gaattaccca actatatctt tgcgctcaac gtcctgtatc agatgggaat ttatgcccac
     3901 gttgatttta tccgtggaca aaattgccaa caggataaca gcacctggga acgttttgag
     3961 cagaacgtca gctggagtct tggcgcgctt aacgacgatg agcgtgagcg gttatatcac
     4021 tggtatcaac aacaagatgc tcgtgccctc gcccctgcca gccgcgactg ggcgctcatc
     4081 tggtgggata gcgtaccgca ggaggcactg tgatgatttt cctttctcag gcacaaatcg
     4141 atgcgttact gctggaagat atccagggag gtgacctgac cacgcgagca ttaaatattg
     4201 gacaccagcc tggctatata gcgttttttc accgtcaggg cggctgcgtc agcggcgttt
     4261 ctgtcgcgtg taagatgtta actacgctgg gattaactat tgatgacgcg gtcagcgacg
     4321 gttcacaagc gaacgcgggt caacggctaa tccgtgcgca gggtaatgcc gcagcacttc
     4381 atcagggatg gaaggcagtc cagaatgtgc tggagtggag ttgcggtgtt tctgattatc
     4441 tcgctcaaat gctggcattg cttcgtgaac gttatcctga cggcaatatc gcctgcaccc
     4501 gaaaagcaat tccgggcacc cgcttactgg cttcgcaggc aattctggct gccggaggac
     4561 taattcatcg tgccggatgt gcggaaacca tattattgtt tgccaaccac cgccattttc
     4621 ttcatgacaa tcagggctgg tcaggcgcaa tcgctcagtt acgtcgccac gcacccgaga
     4681 agaaaattgt tgttgaagcc gacacgccga aagaggctat cgccgcgtta cgcgcacaac
     4741 cagacgtact tcagctcgac aaatttagtc cgcaacaggc aactgaaatt gcgcaaatag
     4801 cgccgtcgct ggctcctcac tgcacgctgg ctctgaccgg cggaattaat ctgacgacac
     4861 tcaaaaatta cctcgactgc ggcattcgcc tttttattac ctccgcgcct tattacgcgg
     4921 caccagctga cattaaagtc agtctgcaac ccacagcctc tatttaattc tttgttaaac
     4981 ggaatttatt ctatgagact caaaaaacgt tatctctgca cagcattatc acttgccttt
     5041 acccagcagg ccgtagcggc tcaggagagc gacacgctga ccgtatggtc cagtccggta
     5101 tcatcgacga cgaccaccgt tctcgatcaa cccaccatga aggccttgga taaacagaat
     5161 gtcgctcagg cattaagtgt cgtccccggc gtggtgctgc aaaagtcagg cagccgcaac
     5221 gaagaacagg taaaagttcg tggctttgat agtcggcagg tgccggtcta tttcgacggt
     5281 gtgcccattt atgtccccta tgacggcaac ctcgatctgg cgcggattct gaccaacaat
     5341 ctgggggcag tcgaagtttc caaagggtat tcgtcgctgc ttcatgggcc taatcagatg
     5401 ggcggagcca ttaatatcac caccctgaag aagcaagtct gggatatcgc cagggatgga
     5461 gccgtagtca ggacaatgcc tacgatatgc atgcttcatt tgccgccagc agcgatcttg
     5521 ggtatttgca agtcagcggt agccagctaa agcaggattt tctcggcctg ccgcatggtg
     5581 taaataatga tattgcaggc aaacacggca agatgattaa ttcatcggct gatgataaac
     5641 gcggcattgt gaagctcggt tttacaccgc gcgaaaacga tgaatacacg ctgacttaca
     5701 ttaagcagga tggtgaaaaa gataacccgc catacagcgg aaattgtggt caaaaatcac
     5761 gctactggca gtggccagag tatgacaaag aaagttttta ttatcaggga gcgacccaac
     5821 taaacgaccg ttttaccctg aaaagtcggc tgtatcgcga cacctttgaa aatacgctga
     5881 tgatgtacaa ctcgctggct gatttgaaaa ataaaaaagg cagctacagc cattattccg
     5941 attacagcga cggtgccgga ttacaactgg cagccgatgt gcgtgaaaac gatctgctgt
     6001 cgtttgccgt taactggaaa gatgacgtac accgggaaaa aggtgcgccg cacgccgctt
     6061 acgatcgcta tgaagatcgt acctggtcgc tcgccagtga atatcaatgg gctgctgctg
     6121 ataatgtcga tgtcgtggcg ggaatcagct atgactggcg cgatagcgta gaagcgaaaa
     6181 aacacgagaa agatggcagc atcacccatt atgacgacaa caatcagtca gcttttaact
     6241 ggcaggtgat ggggaaatac cactttgcca atgaagacac tctggcgctt tcgtactatg
     6301 accgcacacg ctttccgacg ctgaaagaac gctataccac atccaaacct gcgtataacc
     6361 agatagcgat tgttaacccg cagctcaagc cggaacgcgc tcgcggggtg gatttaacct
     6421 ggaatggtgc cttcacgcgc gactggggat ttgaggtcag cgtttactat aaccgggtga
     6481 gtgatgccat cctctcgcac aatatcaatg ccgataccat tcaaaatcag aacagcggca
     6541 cggtggatta cagcggtctg gatgccggta ttaaggggaa aatcagcaat atactggatg
     6601 taggattgag ctacgctctg atccacgctg acgccaaacg caaagacatc ggcaagataa
     6661 ccgatcttcc aacgcagaca atgaccgcat ggatgacact caaaccatgg gagccgttaa
     6721 gcgtaacgct gtcggaagag gcgcgttcct ccagctacag caacagtgac ggttcacaaa
     6781 aagccgccgg ttttgcggtg acccacattc gagccgatta caccttaggt catggcttca
     6841 gcgtcaatgc gtcggtcaat aacctgtttg atacccaata tgcctacagt gaagggttta
     6901 ttgaagaagg tcgcaatttc tgggcaggtg tcgagtatac gttctgatgt gaaagcggat
     6961 agcggcatct gtatcgctat ccgccttatg aaaatcttat tctcttcgca ggaggcggaa
     7021 tacgcccagc accaggatag cgcccaccac cgccaccagg aagctgtgca aattaaaacc
     7081 gctgatggag ccgtcaatgc caaacatggt cgccagccag ccgccgacca ccgcaccgac
     7141 tatcccgaga atacaggtca ggaaaaatcc accaccatca cgccccggca tgattagctt
     7201 gccgataatg ccggctatca ggccaaaaat aatccaggca ataattccca tgttcaggcc
     7261 ctcgtttcga tgaatcggtc gcaagaaatg cttacgtcaa cgctctaagt ataggcaagg
     7321 gcgggatcga tgctgggcgt ttgtcggctg atttaagaac aacatgtaaa aaatattcac
     7381 gcatttgtat taagttttgt taactatgac cgataaacca aagacagttt gtcagtcagg
     7441 agtttttccg cgtgagtcat taccatgagc agttcctgaa acaaaatccg ttagccgtcc
     7501 tgggcgtgtt acgcgatttg cacaaagccg caattccttt gcgtctcagt tggaatggcg
     7561 ggcagctgat cagcaaaata ctggcaataa ccccggataa actggtgctg gatttcggca
     7621 gtcaagccga agacaacatc gccgtgctaa aggcacagca cattaccatt accgccgaaa
     7681 ctcagggtgc gaaagtcgag tttactgttg aacaactaca gcagagtgaa tacttgcagc
     7741 ttccggcatt tattaccgta ccgcctccca ccttatggtt tgtacaacga cgccgatatt
     7801 tccgcatctc cgccccactc catccgcctt atttttgcca gaccaaactg gcggataaca
     7861 gtacgttacg tttccgcttg tatgatttgt cgttaggcgg catgggcgca ttactggaaa
     7921 cagcaaagcc tgccgaatta caagaaggta tgcgcttcgc tcaaattgaa gtcaacatgg
     7981 ggcaatgggg tgtttttcac tttgacgccc agttaatctc caccagcgag cgcaaagtga
     8041 ttgatggcaa gaatgaaacc atcaccactc cccgtctgag cttccgtttt cttaacgtca
     8101 gcccgacggt ggagcggcaa ttacagcgga ttattttctc tctcgagcga gaagcccggg
     8161 aaaaagcgga caaagtgcgc gactgattta catcgcgtcc agtgcctgct caagtttgta
     8221 gatatagcgc ggagcctgcg gcgcaggatg atttcgcgct acgtgatcga ggaactcgtc
     8281 agcatccaaa tcgttgattt tgctgatcgc ctttttccga tctgacgaga aagtccgtag
     8341 cagcgcacct gccccgttag cgtatgacac caccagcgca tattgcagta ccttcggatc
     8401 ttcaatgcct gccagcgggc cggtttccag aatattcagg taagccgccc ccattgaaat
     8461 attacgctct ggatttttca gctcgctggt cgtcggctca ccactccagc ccatacggcg
     8521 ataaacatca cgtccggagg ttgaagcttt taactgcatc aaaccaatgg cattcgattt
     8581 actcaccgcg ttcggattac cacccgattc gatagcgata atcgccgtga tcaattgtgg
     8641 atcgacgccc caggctgcac cggctttctg gcttattggc atccactgca tcgcacgttg
     8701 caccggaact ttcgcgttcc acggcgggtt cgtatagtca tgctttgatg aacaacccgc
     8761 taataacaca atcaaaaagg caaaccatct caatttcact tcttctatcc ttatagcctg
     8821 gaatcgccgt tgaggtgaca acggcacacc taagcggcat gatatacatt tcgtttctgt
     8881 tgtcagcaag gaattgccat gtctctgttt cacttaattg ccccatcggg ttacagcatt
     8941 aaacagcacg ccgcgctgcg tggtatccag cgccttaccg acgcggggca tcaagtcaat
     9001 aacgtcgagg ttattgcccg tcgttgtgag cgttttgcgg gtacggaaac ggagcgtctg
     9061 gaagatctca attcccttgc cagactgact acccccaaca ccatcgtgct ggctgtacgc
     9121 ggcggttacg gtgccagtcg tttactggca gatattgact ggcaggcact ggtggctcgc
     9181 caacaacatg acccgttact catttgcgga catagcgatt ttaccgccat tcagtgcggt
     9241 cttctggcgc aaggcaatgt catcaccttt agcggcccca tgctggtagc aaactttggt
     9301 gcggatgaac tgaacgcctt taccgagcac catttctggc tggcgttacg caatgaaacc
     9361 ttcaccattg aatggcaagg ggaaggcccg acatgtcaga ccgaaggcac gttgtgggga
     9421 ggcaatcttg cgatgctgat ttcactgatt ggtacaccgt ggatgccaaa aattgagaac
     9481 ggtattctgg tgctggaaga tattaacgtg caccctttcc gcgtcgaacg tatgctgttg
     9541 cagctctatc atgcgggaat tttgccacgt cagaaggcga ttattctcgg tagctttagc
     9601 ggcagcacgc ccaatgatta tgacgcaggt tacaacctgg agtcagtgta cgcgtttttg
     9661 cgttcccgcc tgtcgattcc actcattacc ggcctcgatt ttggtcatga acaacgcacg
     9721 gttacgctgc cgttgggcgc acatgccatt ctgaataaca cccgggaagg cgctcagttg
     9781 acgattagcg gtcatcctgt tcttaaaatg taaaaaaatc acgcttcagg gctaagtaaa
     9841 acgctgtctc tgccgctaat atggtagggt ttatataaga gacagcgtaa tcaggagtaa
     9901 ccgaccattg gatgccacaa caataattag ccttttcatc ttaggatcca tccttgtcac
     9961 cagcagtata ttacttagtt cattttcttc ccgtcttggc attcctattc tggttatttt
    10021 tctggcgatc ggcatgctgg cgggagtcga tggcgtcggc ggtatcccgt ttgataatta
    10081 ccccttcgcc tacatggtaa gtaacctggc gctggcgatt attttgctcg acggcgggat
    10141 gcgcactcag gccagttcct ttcgtgtggc gttaggaccg gcgctgtcgc tggcgacgct
    10201 gggcgtactt atcacctctg gtttaaccgg catgatggcg gcgtggctgt ttaatcttga
    10261 tttgattgaa ggcttattaa tcggcgctat cgtcggctcc accgatgctg cagcggtctt
    10321 ttctctgctg ggtggtaagg ggcttaacga acgtgttggc tcgacgctgg aaattgaatc
    10381 cggcagtaat gatccaatgg cggtctttct gacgattacc ctgattgcaa tgatccagca
    10441 gcatgaaagc agtgtcagtt ggatgttcgt ggtcgatatt ctgcagcaat ttggcctcgg
    10501 gattgtcatc ggtcttggcg gtggttattt actgctgcaa atgattaatc gcatcgccct
    10561 gcctgccgga ttatatccat tgctggcatt aagcggcggt attttaattt ttgctttaac
    10621 cacggcgctg gaaggcagcg gtattctggc tgtttatctg tgcggttttc tgctgggtaa
    10681 tcggccgatt cgcaaccgct acggcatcct gcaaaatttc gacggcctcg cctggctggc
    10741 gcaaatcgcc atgttcctag tgctggggct attggttaac ccaagcgatc tgctgcctat
    10801 tgccattccg gcgctcattt tgtcggcatg gatgatattc ttcgcccgtc ccctttcggt
    10861 atttgctgga ttgctccctt tccgcggttt caatctgcgt gagcgcgtgt ttatcagctg
    10921 ggtgggatta cgcggcgcgg tgccaatcat cctagccgtg ttcccgatga tggcggggct
    10981 ggagaatgcg cgactgttct ttaatgtcgc cttctttgtg gttctggttt cactgctctt
    11041 gcagggaaca tcactctcgt gggcggcgaa aaaagccaaa gtggtggttc cgcccgtcgg
    11101 acgtccggtg tcacgcgttg gcctggatat tcatccggaa aatccgtggg agcagtttgt
    11161 ttatcaattg agtgctgata aatggtgtgt aggtgcggca ctgcgcgatt tgcatatgcc
    11221 aaaagagacg cgtattgcag ccctgtttcg tgataaccag ttgcttcatc ctaccggcag
    11281 cacccgactg cgcgaaggcg atgtattgtg tgtaattggt cgggaacgcg atctcccggc
    11341 gctcggtaaa ctgttcagcc agtcgccacc ggtcgcgctg gatcaacgct tctttggtga
    11401 cttcattctc gaagccagcg ccaaatatgc tgatgttgcg ctgatatatg gcctggaaga
    11461 cgggcgagaa tatcgtgata aacagcaaac gctgggtgaa attgtccagc agttgctagg
    11521 cgcggcaccg gttgtcggtg accaggtaga gtttgccggg atgatctgga cggtggccga
    11581 gaaagaagat aatgaagtgt tgaagattgg cgttcgggtg gctgaagagg aagctgaatc
    11641 ttaaatttaa tgcagcatct atcgccggat gcgatgcttt actgaacgca gcctgagcct
    11701 gtaataaact tgtcatttcc tgattttgat atgctcatcc caacatcaga agaaggttaa
    11761 attatggatg acaaaccatt gcaggctctg gctaatgaac tcaaaacacc ggctcaagaa
    11821 agtcagcgta agggcttttt ccgactgtaa caggtaataa aaaccagccc cgcattaccg
    11881 gtcaaccatc aggggcccct gcctgacatc gcctgaacat attcccccac gcaacctgcc
    11941 gcactgacat agcccacaca ataggcgttt catccgcctg tagcaccgga tgcatgagcg
    12001 atttcttata gttattcctt tgcttaataa cgattatttc cgggtcgttc actctcgtat
    12061 tttctttaat acgtcagagc tacctaggcc gtaatacagc atgtaccaga cccgacacgg
    12121 catttctgac caggaaaaaa gtcaacgatg agtagtgata tcagcgcaaa tgacgtcctg
    12181 tggctccttt gcggtgacca cattcacgtc tccggcgctg ccagaggata tttatttcaa
    12241 ggaaacgtag cattatggct agctttaaag acatctgccc attcgccccc gccattcccc
    12301 catcttccgt taacccaggc gtacgacgcc cgccagggcg gctcttctca agaatcaaat
    12361 tttgcagaac atgaagtatc tcagcccgca aaaattcagc tggggtgatg ccccctggca
    12421 gatcattgac ctcagtatcg cgggcaaagt gaacatacag gtggacaaca ataccatcat
    12481 cacgttgggg actcgtctga atcagcaaca taacgagttc atgatggtcg ccaaatggtg
    12541 cgaatgggct atacagcagg atggtttgca agaaaacctg caaaaaaacc tgtacgaaat
    12601 cctggaagaa aaccagcaaa acaaacagtc agaaattccc caagaagacc tgaaagaaag
    12661 tctggaagaa atcaaggaaa atatcctgga agaaaacctg ccagcaagcc ggattgaaaa
    12721 ccgggcagaa gcattgcgca gaatgaagga atgtctcatt acaagacgga gtatgctcaa
    12781 tcttagcaac ctgggattga cttcactccc tgagaatttg cctccacatc tgattgaatt
    12841 ttactgcagt aaaaacgtgt tgaccgcgtt accgaaggtg atgccaaagt ggctgctggt
    12901 gcttgactgt acagataacg tcttgatctt gttaccgaag gtgcagccct caaaactgat
    12961 ggtactgaag tgctatgaaa acagtattat ctggctgcct gagctgtcga ctaacctgag
    13021 agtgattaac tgttctgaaa acttcttgca atttttaccg ccgtcgatgc cccagtacct
    13081 gtataaacta tcctgtgccg gcaacaatat taactcaata cctgatgaga tgctggagaa
    13141 cttgactcgc ctgaaagtat ttgactgttc tagtaacgat ttgatctctt caccacggct
    13201 gccgcccaaa ctaataattt actactgtgg agaaaacaag tttaaaacgg tacaggtgcc
    13261 gcagccccag agcctgaagg tgtttgactg taatggtaac ccgtgggaca aagacaattt
    13321 accgacgctg ctcaaagccg tcgagggcct gaaaaaacag cagggcctga aggacctttt
    13381 ggactttttg cacaaggaag gctaggttga cctggaagga ctcgaggagc tggaggacct
    13441 tatggatctg gagttcctgg atgacccgga actcctggag cgcgtgaagg tacaggagga
    13501 cctggagctc ctggatcaac agttgggcct gttgagtctg gaaagacaac aggactcgca
    13561 gcctgttaat caacaatctg aacatgaacc cgaatctgca tcaagggtga agcgtgattt
    13621 atctgaagtc gactccgagt caacaatgaa gcgtaagcgt tttatgtaga acgcctgtgc
    13681 ggcgagttca ggttccgggg aaatagtgtc cattctttta atggacacta tcatgggcaa
    13741 tggctctgtc acattaacaa ctacagttgc tgaccagccg gatggatgat tttttcgcaa
    13801 ccagaagacg ctgcctccga tccggcttac aacaagttac actgtcacaa ccgggacgcg
    13861 taacgccagt gcgcacatca actcatagcc caccgttccg gcagcggcgg cgacatcatc
    13921 aattttgatc tccttgcccc acagctcaac cggcgtacca atccccgcct gcgggcaagg
    13981 cgttaaatca accgccagca tatccatcga gacggtcccc accgtcatgg tgcgcacgcc
    14041 gtccactaaa acaggagcac cggtaggcgc atggcgcgga taaccgtcgg cgtatcctgc
    14101 ggcgacaatg ccgattcgct gttcatcgcg cgcagtatag cgaccgccgt agcccacacg
    14161 ctcgcccgct tttagcgtct ggacaccaat aatctcactg cttagcgtca tcaccggacg
    14221 taatccggta ttggcgatat cacgccactg accggacggc gaagcgccat acaaaataat
    14281 gccaggccga acccagtcaa aatgcgcttc cgggtgccac agagtcgccg ccgaattgga
    14341 caacgaacgc cgacactcca gcccctccgc cgcctgctca atacgcgcca tcgcgccgga
    14401 aattccatca ggatgttccg cttcagcaaa atgcgacatc agggtcattt cgccaacatt
    14461 cgccattgcc cgcaactgct gccagacggt aagcacgcga tcggactgga agcccaaccg
    14521 attcatccca ctgttcactt taagataaat atccaacggt gcttttagcc gcgcattttg
    14581 cagtgctttg agctgccagt tgctgtgtac gcaggtggtc aggcggtgct ggtcataaat
    14641 ctccagatcc tgagcatgga aaaatccttc cagcatcagg atcggcccct tccagccgcg
    14701 ctcacgtaac gttattgcct cttccaggtt aagtaatgca aagccatcgg tggccccgag
    14761 cgcgctccag atacgctcaa taccatgccc gtaagcgttc gcttttacca ccgaccagac
    14821 gcgcgcgtgc ggcgcggcct ggcggacaat ggacagattc tgttttaatg cctgcagatc
    14881 gaggctggcc tgtatcggac gggtcatctc gtttccttag ctgtgtgcgc catgtaaatg
    14941 gcccggacgt gatggcgtaa atccacggct gtagcgcgct acgcttagat cctcatatgg
    15001 gatcgctggc gtgcgaccag agagcagatc gcttaacaac tgaccggaac cgcaagccat
    15061 cgtccagccg agcgtgccgt gaccggtatt cagccacaga tttttaaagc gtgtactccc
    15121 gacaaccggc gtaccgtctg gcgtcatcgg gcgcaaaccc gtccagaaag tcgcctgctc
    15181 gacatgaccg ccgcgtggat agagatcgcg aaccaccatt tccagcgttt cacgacgcgg
    15241 ttgcaacagc tcggtgttaa aaccaacaat ttccgccatt ccgccaacac gaatgcggtt
    15301 atcgaaacgg gtaatggcga ttttgtaggt ttcatcaaga atggtggata ctggcgcacc
    15361 atcttcctgc gcaattggaa tagtcagcga gtagcctttc agcggataca ccggaatatc
    15421 aacaatgcct ttgagcatcg ccgtcgagta agaaccaaac gccatcacat acgcatcggc
    15481 cttaatcact tcatcgccaa acttcacgcc gtagatttgc tcgccgtcgc aaagcagttg
    15541 gtcaacgggc gtattaaagc ggaatttaac ccccgcctgc tctgccatcc gcgccagatt
    15601 ctgggtaaac agctggcagt ctccggtttc atcattgggt aactgcaggc cgcccgtcag
    15661 tttgtgcgct acttctgcca gcgcgggatc cacttccgcc aggcggctgg attccagcag
    15721 ctgatacggt acgccggcat cttccagcac ggcgatatcg cgggtcgcat tttcatactg
    15781 ttgttcggta cggaacagtt gcagcgtccc accctgacgc ccttcatact gaatattggt
    15841 ttcggcgcgc aatgctttca ggcaatcacg gctgtattcc gccagacgca ccatccgccc
    15901 tttgttttcc atgtagtggc tggtgtcgca gttacgtaac atttgccaca tccatttcaa
    15961 ctgaaactgc gtaccgtcga gacgaaccgc cagcggcgca tggcgctgga acatccattt
    16021 aatcgctttt aaaggcacac ctggtgctgc ccacggtgca gcatatccgg gggagatttg
    16081 cccggcattc gcggcactgg tttccagggc ggcccccggc tcccgatcaa tgacggtgac
    16141 ctcatgtcct gcctgattta agtaccaggc gctggcaacg cctaccacac cacttcccag
    16201 tatgacaact cgcatagcca ctccgataac ggtagataca gtaaaagaat aataatctaa
    16261 ttacatcttg ataactcagt tgaaaatatt attcaacata cggctttttt atggtgagct
    16321 gactcacatc tccctgactc tgcggcaata tggcgtcttt agtatctcct gctgaacgcc
    16381 ctttttatcc agcataaaat tccgttcaga agcggattag tggcactctg ataataagaa
    16441 atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac aggtgttcta tgcttgaaat
    16501 gaggtgctcc acacagagag cgccgacaac aatgaggtgc gcgtatggcg acgatcgatt
    16561 ctatgaataa ggacaccaca cgtttgagcg atggacccga ctggacgttc gacctgctgg
    16621 atgtttatct ggcagagata gaccgggtgg cgaaactcta ccggctggat acctacccgc
    16681 accagattga agtgataacc tcagaacaga tgatggatgc ctattccagc gtcggcatgc
    16741 caattaacta tccgcactgg tcattcggta aaaagtttat cgagactgaa cggctgtata
    16801 agcacggtca gcaagggctg gcctatgaaa tcgtcattaa ctctaacccg tgtatcgctt
    16861 acttgatgga agagaacacc attactatgc aggcgctggt gatggcacac gcctgctatg
    16921 gacataactc tttctttaaa aacaattgct tattccgcag ttggaccgac gccagttcga
    16981 ttgtcgatta tctgattttt gcccgtaaat atattaccga gtgcgaagaa cgttatggcg
    17041 ttgatgaagt agaacggctt ctggactcgt gccacgcgct gatgaactac ggcgtggacc
    17101 ggtacaaacg cccgcaaaaa atctcgctgc aagaagagaa agcccggcag aaaagtcgcg
    17161 aagagtatct acaaagtcag gtcaatatgc tctggcgtac cctgccgaag cgcgaggaag
    17221 agaaaacggt tgctgaagcg cgccgctatc cgtccgaacc acaagaaaac ctgctctatt
    17281 ttatggagaa aaatgcgcca ctgctggaat catggcagcg tgaaatcctg cgtattgtgc
    17341 gtaaggtgag ccagtatttt tatccgcaaa aacagactca ggtgatgaac gaaggctgtg
    17401 cgaccttctg gcactatacc atccttaacc atctgtatga tgaagggaaa gtaacggaac
    17461 gttttatgct ggagtttttg cacagccaca ccaatgtggt cttccagccc ccctataaca
    17521 gcccgtggta cagcggcatc aacccgtatg ccctcgggtt cgccatgttc caggatatta
    17581 aacggatttg tcagtcgcca acggaagaag acaaatactg gttcccggat atcgccggtt
    17641 ccgactggct ggaaacgctg catttcgcga tgcgtgattt caaagatgag agttttatca
    17701 gccagtttct gtcaccgaaa gtgatgcgtg atttccgctt cttcaccgtg ctggatgatg
    17761 atcggcataa ttatctggag atttccgcta ttcataatga agaaggttat cgggagatcc
    17821 gtaaccggtt atcgtcgcaa tataacttaa gtaatctgga gccgaatatt cagatctgga
    17881 acgtggattt gcgcggcgac cgttcgctga cgctgcgtta tattccacat aatcgcgcac
    17941 cgctggatcg ggggcgcaaa gaagtcctga agcatgtgca tcgcctgtgg ggatttgatg
    18001 tgatgctcga acagcaaaac gaagacggca gcatcgagtt gctggaacgt tgcccgccaa
    18061 gaatgggaaa tctgtaaaaa caacaaaaaa cccctcgttt gagggggttg ctctttaaac
    18121 ggaagggatt atcgcccctg aatggctaaa tcacccggca gatttttctg catccggtgc
    18181 caaatctcgc cactctcatg cccatagcga cgcactgttt cgtacacctg atcgtgcgcg
    18241 ccttcactgc acaacgccga cagtttgtgg tagaagccca gcgccagact gcgcgcttcc
    18301 ggattggcga aatagtgacg accaatgcgc gtatacagcc ctttcatccc gttaaggatc
    18361 agaccgtaaa tcgggttgcc ggaagcaaac gccaggccgc ggaatatgtt gtaatccagc
    18421 tcggcaaagg catcggcgtg atcggccact tcattagcgg tagccagcac ttcctgcgct
    18481 ttatcgggat gctgacgaaa cgcggtgcga ataaaaatag tggaaatatt ggtacgcacc
    18541 gacagcaaat tatcaataag ctgcggcaca ctttcgtgat ccagtcgcgc cagtgtttca
    18601 aggatattta aaccggaagt ttcccagaaa ttattcacct tcgtcggctt gccatgttga
    18661 atggtcaacc agccatctcg tgccagacgc tgtaacactt cacgtaacgt agtacgcgtt
    18721 acgccaatta attctgaaag ttcacgttct gcgggcaaaa tagtcccggg agggaagcgg
    18781 ttattccaga tactttcaat aatgtactct tccgcgaaac ccgccgggct ttgcgcctta
    18841 atgaccatag tgagatttcc ataacacagc aaaacaaagt tggactcatc ataccagagg
    18901 gctaactacg ctgatagcag acaaaaagaa gaaaaaggga tcaaggcttc attttccgca
    18961 tctctttccc cttgactatc aagacttgcc tcactcctgc ctgaccgtta agctttgcgt
    19021 ttaaacataa aaacatcatt gattatttgt aaggtaaggg aaaccatcat ggagatctcc
    19081 tggggccgcg cgctatggcg caattttttg ggccagtccc ccgactggta caaactcgcc
    19141 ctcatcattt tcttaatcgt aaacccgtta attttcctca tcagcccttt cgtcgcgggc
    19201 tggttgctgg tagcggaatt tattttcact ctggcgatgg ccctgaaatg ctacccgcta
    19261 ctccccggtg gtctgttggc tatcgaagcg gtattcatcg gcatgaccag cgcggaacac
    19321 gtccgtgaag aggtggcggc aaatcttgaa gtcttgctgt tattgatgtt tatggtggcg
    19381 ggtatctatt ttatgaaaca gctgttgctg ttcatattta cccgtttgct gttaagcatt
    19441 cgctccaaaa tgctgctgtc gctctctttt tgcgtggcgg cagcgttcct ctccgcgttc
    19501 ctcgatgcct taaccgtcgt ggcggtggtg atcagcgttg cagtcggttt ttatggtatt
    19561 tatcatcgcg tagcctcttc ccgtaccgaa gacaccgacc tgcaagacga tagtcatatc
    19621 gacaagcatt acaacgtggt tctggaacag ttccgtggct ttctgcgtag cctgatgatg
    19681 catgccggtg tcggcaccgc attaggcggc gtaatgacca tggtgggcga accacagaac
    19741 ctgatcatcg ctaaagcggc tggctggcat tttggcgatt tcttcctgcg catgtcgccg
    19801 gtgaccgttc cggttctgat ttgtggcctg ttaacctgcc tgctggtaga gaagctgcgt
    19861 tggtttggct acggtgaaac gctgccggag aaagtccgcg aagtgttgca acagtttgac
    19921 gatcaaagcc gccaccagcg tacccgtcag gataaaatcc gtctgattgt ccaggcgatt
    19981 attggcgtct ggctggtgac tgcgctggcg ttgcatctgg cggaagttgg cttgattggt
    20041 ttgtcagtca ttattctggc aacatcattg accggtgtca ccgatgaaca tgctatcggt
    20101 aaagccttca ccgaatctct gccattcacc gcactgttga cggtgttttt ctcggtagtc
    20161 gcggtgatta tcgaccaaca actgttttcg ccgattattc agtttgtgtt gcaggcatct
    20221 gaacatgctc agctgtcgct gttctatatt ttcaacggtc tgctgtcatc catttcggat
    20281 aacgtcttcg tggggacgat ttatatcaac gaagcgaaag cggcaatgga aagtggcgct
    20341 atcacgttga agcaatacga gctgctggcg gtcgccatta ataccggtac caatctgccc
    20401 tctgtcgcta cgccgaacgg tcaggctgcg ttcctgttcc tgctgacctc tgcactcgcg
    20461 ccattgattc gcctctctta tggccgcatg gtgtggatgg ccctgcctta caccctcgta
    20521 ctgacactcg tcggcttgct ctgcgtcgag tttacgcttg cccctgtaac cgaatggttt
    20581 atgcaaatgg gctggatagc aacgctttga taacaactta ccgggcatat aaatgcccgg
    20641 tttgcctttt cgcccgataa ttgtccaatt gcgtcctatt tttgctgcct cctggtggcg
    20701 cagcgaatga attggtttaa actgcgcact ctatgcatat tgcagggaaa tgattatgtt
    20761 gcgatttttg aaccaatgtt cacaaggccg gggcgcgtgg ctgttgatgg cgtttactgc
    20821 tctggcactg gaactgacgg cgctgtggtt ccagcatgtg atgttactga aaccttgcgt
    20881 gctctgtatt tatgaacgct gcgcgttatt cggcgttctg ggtgctgcgc tgattggcgc
    20941 gatcgccccg aaaactccgc tgcgttatgt agcgatggtt atctggttgt atagtgcgtt
    21001 ccgcggtgtg cagttaactt acgagcacac catgcttcag ctctatcctt cgccatttgc
    21061 cacctgtgat tttatggttc gtttcccgga atggctgccg ctggataaat gggtgccgca
    21121 agtgtttgtc gcctctggcg attgcgccga gcgtcagtgg gattttttag gtatggaaat
    21181 gccgcagtgg ctgctcggta tttttatcgc ttacctgatt gtcgcggtgc tggtggtgat
    21241 ttcccagccg tttaaagcga aaaaacgtga tctgttcggt cgctaatcca ttcggcgctc
    21301 ctgcgggagc gcttttttcc tgccgctata tttatttgac ccgcagtaaa tcagaacttc
    21361 gcgttgtata acgtggtgaa agcatagctc gcttcatctg ccattgttgt tggatcccct
    21421 gcccggcaaa atagagtgtt cctctgccct ctttagcatt cagagtatcc attaccgcca
    21481 tcaattgctc actcccgggg cgcggtgcgt tgtcatcgaa taaattgagc tgcgcgactc
    21541 cctgactgaa gaaatccccc agcatcacgc ccgctttttg gtagcgatgg cccgcttgcc
    21601 agatggcatc cagagatcgc gtagcagcgt taatgatatc cctgctgtcc tgagtgggcg
    21661 tcagcagttt taccgacgcg ctattgccgt aataaggttc attgagcgca aatggtgacg
    21721 tcttaataaa tgtggagata aaccgacaat attgatgctc actgcgaagt ttttccgccg
    21781 cccgggcagc gtaactacaa atggcctgcc gcatcgacgt ataatccgtg atgcgttcac
    21841 caaacgagcg ggaacagata atttcctgct tcgtcggtgc aaactcttcc agttgcaaac
    21901 agggttcgcc gcgcagttca cgcaccgttc tttcgagcat gacattaaaa tgtttacgga
    21961 taaaccggat atctgtatcc gccaaatcga gaacggtttt gatccccatc gcgtccagtt
    22021 ttttgctgat ccgccgtcca atcccccaga cgtcatccac ggggagagca gacattaatt
    22081 tacgctggcg ttccagattt gataaatcca ccaccccacc cgtctgccgc tgccattttt
    22141 ttgccgcatg attggcaagc ttagccaacg ttttggtctg ggcgatcccc acaccaacag
    22201 taagatgggt acgttgtagc accgttgcgc gaatttctct gccaaaatca gtcagatcgc
    22261 gacaattacg cacacctgtc agatcgcaga atgcttcatc aatactgtaa atctcgacgc
    22321 ggggcgatag ttcttccagc gtcgacatca cccgattgct catgtctgcg taaagctcat
    22381 aattgctgct aaagcaaacc acaccacagc gacgaaacag atctttttgt ttgaaccagg
    22441 gatcgcccat tttaacgccc agcgcctttg cctcagcgtt tcgggcgata acgcaaccgt
    22501 cattattcga tagcacaacc accggtttac cccataaatc agggcgaaac accgtctcac
    22561 agctggcata aaacgcgttt acatcacaga gggcaaacat cagcgcatcg ccttaacgac
    22621 gtggatcacc acaccaaaga catccagcgt atcttcgcta ctgatggtaa tgggcgagta
    22681 cgcactgttc atgggaataa gctgtaccgt cgggcgtagt tgcaattttt tcaccgtaaa
    22741 ctcgccgtca acagcagcga tgacaatatc accatggctg gcggtaatag cactatcgac
    22801 aatcagtaaa tcaccgtcac taattccacc atcaatcata gaatcaccac ttgctttgac
    22861 gaagtaagtc gcgctgggat gctggatcaa cagttgattc agatcgatgc gctgttcaac
    22921 gtaatctgct gccggtgaag gaaagccaca ctgaacaaga tcgctaaata gcggaaaagt
    22981 cacaatttcg cggagatccg caggcttgat aaacaacata ataatctgcc tgaagttata
    23041 ctgtttttat atacagtggt ctgttcttgc cagcagatca atactgattc aggctatcaa
    23101 tatttgtcgc tgcatagact gctgattttt cgttctctta tcctgtgctc acgtggcctt
    23161 ctggcgacga cgctcatcca gcagaaatga aaaatatcac ccggcaaaaa aatagaatag
    23221 aagcattcgc cataatgaca ttaaacattg tttgatattt atcatattaa tagaaataaa
    23281 gacattgacg catcccgccc ggctaactat gaattagatg aagtaaaatt tattaatagt
    23341 tgtaaaacag gagtttcatt acaatttata tatttaaaga ggcgaatgat tatgactgaa
    23401 atcgttgcag ataaaacggt agaagtagtt aaaaacgcaa tcgaaaccgc agatggagca
    23461 ttagatcttt ataataaata tctcgatcag gtcatcccct ggcagacctt tgatgaaacc
    23521 ataaaagagt taagtcgctt taaacaggag tattcacagg cagcctccgt tttagtcggc
    23581 gatattaaaa ccttacttat ggatagccag gataagtatt ttgaagcaac ccaaacagtg
    23641 tatgaatggt gtggtgttgc gacgcaattg ctcgcagcgt atattttgct atttgatgag
    23701 tacaatgaga agaaagcatc cgcccctcat taaggtactg gatgacggca tcacgaagct
    23761 gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg cttccgggaa
    23821 actgctggcg ttagatagcc agttaaccaa tgatttttca gaaaaaagca gctatttcca
    23881 gtcacaggta gataaaatca ggaaggaagc gtatgccggt gccgcagccg atgtcgtcgc
    23941 cggtccattt ggtttaatca tttcctattc tattgctgcg ggcgtagttg aagggaaact
    24001 gattccagaa ttgaagaaca agttaaaatc tgtgcagagt ttctttacca ccctgtctaa
    24061 cacggttaaa caagcgaata aagatatcga tgccgccaaa ttgaaattaa ccaccgaaat
    24121 agccgccatc ggggagataa aaacggaaac tgaaacaacc agattctatg ttgattatga
    24181 tgatttaatg ctttctttgc taaaagcagc ggccaaaaaa atgattaaca cctgtaatga
    24241 gtatcagaaa agacacggta aaaagacact ctttgaagta cctgaagtct gataagcgat
    24301 tattctctcc atgtactcaa ggtataaggt ttatcacatt taaaaacaca gacgtttttt
    24361 aaagattgag agttacctgt tgataataag gtatcgggta actctcatta tgacagagcg
    24421 ctttcaaaaa cagggctacg aaatggcgag catccagacg tatattaaga aagcccccat
    24481 cagcatctat taaatttatc actgcgccca gtcaggttta tttaagatat gatcctgcca
    24541 gtcaatcact tctgattctt tcactgcgat atgacgcaca gagatacgtt caccatgcat
    24601 tgccgctttc gaaccagtaa gtagcggatg ccacgcaggt aaatctttac cttccgccag
    24661 caaccgataa gcgcaggtca ttggcagcca ttcgaatgtt ggcagatttt cacgggttaa
    24721 tttaatgcag tcgggttcaa actcgaaacg acgttcgtag ttccgacatt gacaggtttt
    24781 aatattgagc tggcgacagg cgacgttagt gaagtagatt tcgtcggtgt cttcatccat
    24841 cagtttatgc aagcaacact gaccgcaacc atcacacaac gactcccatt ccgcatcgct
    24901 catttcgtcc agggttttac tttgccagaa aggtacatcg ctcatcaggt gttccgccat
    24961 tacgttaaaa cgcaccttat aaccagtctg gcacagcgat gcaagttttg ccgccgcttt
    25021 ctggcggcaa aaagtattac aaaacgcgag ttgtcaaaga atgcccatcg aaagtgactg
    25081 tcagctcatc accgctttgc aacgggccga cgccatcagg cgtgcctgtc aacacaacgt
    25141 caccggcctt gagggtaaaa aacttgctca tataagcgat caacggaacg attttatgga
    25201 tcatgtccgc agtcgtacct tgctggcgtt tttcgccgtt tacgctcagg cccagcgttg
    25261 tattttgtgg atcgccggta aattccgccg cggggataaa cccggaaagc ggacaagagt
    25321 tatcaaacgc tttagccttt tcccacggct gcccggcttt cttcattttt ccctgaacat
    25381 cacgcaatgt cagatcaagc gccacgccgt aaccggcaat ggctttg
//
LOCUS       DAJVQR010000056        24892 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVQR010000056 DAJVQR010000000
VERSION     DAJVQR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..601)
                     /gene="yeaG"
                     /locus_tag="OQM52_002423"
     CDS             complement(<1..601)
                     /gene="yeaG"
                     /locus_tag="OQM52_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1564238.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVW"
     gene            1037..1783
                     /gene="mipA"
                     /locus_tag="OQM52_002424"
     CDS             1037..1783
                     /gene="mipA"
                     /locus_tag="OQM52_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1564239.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            1873..2634
                     /locus_tag="OQM52_002425"
     CDS             1873..2634
                     /locus_tag="OQM52_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1564240.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(2685..3569)
                     /locus_tag="OQM52_002426"
     CDS             complement(2685..3569)
                     /locus_tag="OQM52_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1564241.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(3653..4648)
                     /gene="gapA"
                     /locus_tag="OQM52_002427"
     CDS             complement(3653..4648)
                     /gene="gapA"
                     /locus_tag="OQM52_002427"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1564242.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            4990..5403
                     /gene="msrB"
                     /locus_tag="OQM52_002428"
     CDS             4990..5403
                     /gene="msrB"
                     /locus_tag="OQM52_002428"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1564243.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5445..5717
                     /locus_tag="OQM52_002429"
     CDS             5445..5717
                     /locus_tag="OQM52_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1564244.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6087..7163
                     /locus_tag="OQM52_002430"
     CDS             6087..7163
                     /locus_tag="OQM52_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1564245.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7190..8569
                     /locus_tag="OQM52_002431"
     CDS             7190..8569
                     /locus_tag="OQM52_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564246.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            8586..9631
                     /locus_tag="OQM52_002432"
                     /pseudo
     CDS             8586..9631
                     /locus_tag="OQM52_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            9652..10488
                     /locus_tag="OQM52_002433"
     CDS             9652..10488
                     /locus_tag="OQM52_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCS1564247.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            10493..11440
                     /locus_tag="OQM52_002434"
     CDS             10493..11440
                     /locus_tag="OQM52_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCS1564248.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            11450..12430
                     /gene="ydjG"
                     /locus_tag="OQM52_002435"
     CDS             11450..12430
                     /gene="ydjG"
                     /locus_tag="OQM52_002435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCS1564249.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            12567..13325
                     /locus_tag="OQM52_002436"
     CDS             12567..13325
                     /locus_tag="OQM52_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1564250.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            13443..14801
                     /locus_tag="OQM52_002437"
     CDS             13443..14801
                     /locus_tag="OQM52_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564251.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(14894..15535)
                     /gene="pncA"
                     /locus_tag="OQM52_002438"
     CDS             complement(14894..15535)
                     /gene="pncA"
                     /locus_tag="OQM52_002438"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1564252.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(15546..16562)
                     /gene="ansA"
                     /locus_tag="OQM52_002439"
     CDS             complement(15546..16562)
                     /gene="ansA"
                     /locus_tag="OQM52_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1564253.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(16729..18585)
                     /gene="sppA"
                     /locus_tag="OQM52_002440"
     CDS             complement(16729..18585)
                     /gene="sppA"
                     /locus_tag="OQM52_002440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1564254.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            18746..19297
                     /locus_tag="OQM52_002441"
     CDS             18746..19297
                     /locus_tag="OQM52_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1564255.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19414..20457
                     /gene="selD"
                     /locus_tag="OQM52_002442"
     CDS             19414..20457
                     /gene="selD"
                     /locus_tag="OQM52_002442"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1564256.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            20462..22423
                     /gene="topB"
                     /locus_tag="OQM52_002443"
     CDS             20462..22423
                     /gene="topB"
                     /locus_tag="OQM52_002443"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1564257.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            22550..23590
                     /locus_tag="OQM52_002444"
     CDS             22550..23590
                     /locus_tag="OQM52_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1564258.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23707..>24892)
                     /gene="gdhA"
                     /locus_tag="OQM52_002445"
     CDS             complement(23707..>24892)
                     /gene="gdhA"
                     /locus_tag="OQM52_002445"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1564259.1"
                     /translation="LVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRF
                     HPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELY
                     RHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGY
                     GLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDE
                     SGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDV
                     DAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQN
                     AARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQG
                     VI"
BASE COUNT         6341 a         5787 c         6422 g         6342 t
ORIGIN      
        1 gccagacctt cactacccgg aacttagtga tatcgccacc aaattcatgc aggcgttttg
       61 ccgcccatgg cgacatgatg gtgccgagat aacggcgagg aatgccatac tctttttcca
      121 gaatctgagc atcttcctgc ggattgaaaa gacagaacgg atgatcgttg accgggctac
      181 gctcaccgtt ggcgctcaat acataaatcg gtacgagctg cattaatgat ttcagtcgct
      241 cagcaagcga tgatttaccc ccacccacag gccccagcag atacaggatt tgtttcttct
      301 cttccagccc ctgagccgcg tgtttcagat aagagacaat ctgttcaatc gcgtcttcca
      361 tgccgtaaaa ctcttcaaat gccggataac gtgcaatgac ccggttagaa aagagtcgag
      421 aaagtctggg gtcctgggct gtatcgacca tgacaggctc accgatagcc atcaatagcc
      481 gctcagccgc gttggcataa gcactgcgat cttgccgaca agtggtaaga aactcctgca
      541 gtgtgaactc ttcgtccttg gcagcttcat atcgctggcg atagtgatcg aatatattca
      601 tggtatgccg tcctttcgtt ttttagcaca ggttaagagc cgttcatatg aatagtagag
      661 gctcccggaa gaggtaaact gaacgacgtc tcttgctaaa acacagcaac tctcatgcca
      721 ttttatcgac gatgagaaca acgtgcccgg tgatacacct tcaaaattaa gcgtagatgg
      781 caaatggaaa acttgcctgc acggctttct taatttcaat ggcatatcaa caactcatcc
      841 ataaattttg cataattaat gtaaagacca ggctcgccag taacgctaaa tccatttggc
      901 tgtaagcgcg gtgtcatccg cggcaggaaa attaaacagt tactttaaaa aatgaaaacg
      961 taaaaaggtt gggtttcgat gtattgacgg gtaaactttg tcgcccgcta aacatttgtt
     1021 taaggaatga ttaattgtga ccaaactcaa acttctggca cttggagtgc ttatcgcaac
     1081 gtctgcaggc gtagcgcacg ctgaaggtaa attttccctg ggcgcaggcg taggtgtcgt
     1141 tgagcaccca tataaagatt acgataccga tgtttaccca gtaccggtaa tcaactatga
     1201 aggcgataac ttctggttcc gtggcttagg tggtggttac tacctgtgga atgacgcaac
     1261 ggataaactt tcaattaccg cttactggtc gccgctttac ttcaaagcga aagacagtgg
     1321 cgatcaccaa atgcgtcacc tggatgaccg taagagcacc atgatggctg gtctgtctta
     1381 tgctcacttt acccagtacg gttacctgcg taccaccctg gctggcgata ccctggataa
     1441 cagcaacggc attgtctggg atatggcctg gttgtatcgt tacaccaacg gtggcctgac
     1501 cgtgactccg ggtattggtg tgcagtggaa cagcgaaaac cagaacgaat actattatgg
     1561 cgtatcgcgc aaagagtccg ctcgcagcgg tctgcgtggc tataacccga atgacagctg
     1621 gagcccttac cttgagctga gcgccagcta caacttcctc ggcgactgga gtgtttacgg
     1681 taccgcgcgc tacacccgtc tgtctgatga agttactgac agcccgatgg tggataaatc
     1741 ctggactggc ctgatttcta ccgggatcac ctacaaattc tgataatgca tctttccagg
     1801 gcattttgtg cataaaaaca gggcgtcacg ccctgttttg cattacggtg gtgcaatacc
     1861 tgggaggaaa caatgcaaca aaaaatgatt caatttagtg gcgatgtctc actgccagcc
     1921 atagggcagg gaacatggta tatgggcgaa gatgccagtc agcgcaaaac agaagttgct
     1981 gcactacgcg caggcattga actcggttta accctcattg ataccgccga aatgtatgcc
     2041 gatggcggtg ccgaaaaggt ggttggggaa gcattaaccg gtctgcgaga gaaggtcttt
     2101 ctcgtctcta aagtctatcc gtggaatgct ggcgggcaaa aagcgataaa tgcatgcgaa
     2161 gccagtttac gccgtctcaa tactgactat ctcgatctct acttattaca ctggtctggc
     2221 agtttcgctt ttgaagagac tgttgctgcg atggaaaaat tgatcgccca gggaaaaatc
     2281 cgtcgctggg gcgtttctaa ccttggttat gccgatatgc aggaactctg gcagctgccg
     2341 gggggaaatc agtgtgccac taatcaggtg ctttaccatc tcggttcacg agggattgag
     2401 tacgatctac tcccctggtg ccagcaacag cagatgccgg tgatggctta cagtccatta
     2461 gcccaggccg ggcggttgcg caatggactg ttaaaaaacg cggtagtcaa cgaaattgca
     2521 catgtccaac aacatgcagc tgcgcttgag gtcgaacttt cgtcagcgga attagctatg
     2581 ctggataagg catatccggc accaaaagga aaaactgcgc tggatatggt gtgatgttga
     2641 tacgtgccgg atgcatcgca cccggcacac aacattaacg caaattaacg tttggcaacg
     2701 cgaatggatt gcgccagatg tgcaggtttc tctttggtga ctttttgcgt ttctgaagcg
     2761 taagccgttt ctacacaaac aaaggtcttg tagccatcgt ccggcatgtc gcccatgcta
     2821 actgaaagcg ccggtcccgg gttccagccg acaacgttca gatgatgctg gtggcctacg
     2881 gcgataatac gattcagcgc ttcatcatta atcacgctgc aatcttgcgg attcagatac
     2941 acgcgatcgg tacggtcagg gaaggtctga ataccgtcgg tcagtacatc ttctttcgcg
     3001 tcattcactt tatcaatgaa gcgatcgccc agcccactga cgcttacctt agcgatatca
     3061 cccacgttaa agtaggtatg cagggcagag gtggtttcaa attcgccatg tgattcaaga
     3121 tcgatttcac aggttttacc cacgcggaaa tgcgctaaca gcgtaaagtc gtgcggccag
     3181 aattttttcg tctcttcgct ttgtgtcaat tcaaaagtca gcgctacgcc atcagcatct
     3241 tcatgatgtg atttcagcgt ccacggcagg ttgcgggcaa aaccgtgcgc aggcagacct
     3301 tgttgtgccg ccggaccaaa ccacggccag caaaccggta cgccaccgcg aatagcgacg
     3361 ccatttttga acggtgtgtt gttgctcaac cacagaactt cttcttcacc cgcaggtttc
     3421 cacgagagaa ggtgtgcgcc ctgtaatgca aaagaggctt ttacctgggg atgatcgacc
     3481 acaatgaggt ccagttcatc cagtttacga cgggagagga caggggagat ttgttcgatg
     3541 accggaaggg caaaaatttt cttaatcatg acgcagtcct ttaacttcat tttatcaggt
     3601 aaaaaaaaga gcgaccgaag tcgctctttt tagatcacag tgtcatctca acttatttgg
     3661 agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg
     3721 ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag
     3781 tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc
     3841 ctttcatttc gccttcagca gcagctttaa cggcagcttt gatctgctcg taagttgcag
     3901 ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg
     3961 ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac
     4021 cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag
     4081 acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt
     4141 cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc
     4201 aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga
     4261 acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga
     4321 tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa
     4381 cacccacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt
     4441 taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag
     4501 tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa
     4561 cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa
     4621 aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa
     4681 ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg
     4741 cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactt ttcctttggg
     4801 cttgagacca catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga
     4861 ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc
     4921 cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga
     4981 tgtgagtaaa tggcgaataa accttcggca gaagaactga aaaaaaattt gtccgagatg
     5041 cagttttacg tgacgcagaa tcatgggaca gaaccgccat ttacgggtcg tttactgcat
     5101 aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattccgaa
     5161 accaagtatg attccggctg tggctggccc agtttctacg aaccggtgag tgaagaatcc
     5221 attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac
     5281 tgtgatgccc atctggggca tgtctttccc gacgggccgc aaccaacagg cgaacgttat
     5341 tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga aatcaacggt
     5401 tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca
     5461 ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga
     5521 aatggcctga tggcgttgcg ttgaccgagg aacaaaaaga gaattgcctg caactggtga
     5581 tgctgtggca agcccgccat aataccgaag cacagcatat gacaattgac acaaatggtc
     5641 agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta
     5701 ttgccatgtt taagtaagtg gttcatcccc tttttgccgc ggctatgaag gggatagcca
     5761 acaatgaccc cacgactcgt ctcaattatc tcgacccgac ctggctaagt cccagtccga
     5821 tctcttgcag tcgcgttcac gcctgaatga cttctcctga acttaagtct cagacctatt
     5881 tggccggtaa tccctctcga atttgctctt tttgcgtccc gccttccacc aacctgtaac
     5941 agacccagcc attttctgcg taattgtgat ttgagttgta aaatgcaggt cattatctgt
     6001 gttgtgcaga tttaatattg acctcaaaaa tcatgtgggt aagtatgatg gccatctaag
     6061 caaaacaaaa cacagaggat gacgcaatga aagcactggc tcggtttggc aaggcctttg
     6121 gcggctacaa gatgattcat gtcccacaac ccatttgtgg cccggaagac gtagtgactg
     6181 aaattaaagc cgcggcaatc tgcggcgcag acatgaagca ctacaatgtc gatagcggtt
     6241 ctgatgagtt taactctatc cgcggccatg agttcgcagg ttgtattgcg caggttggtg
     6301 aaaaagtcaa agactggaaa gtggggcaac gcgtcgtgtc ggataacagc ggtcacgttt
     6361 gcggcgtttg tccggcctgt gaacagggtg attttctgtg ttgtacagaa aaagtaaacc
     6421 ttggtctgga taacaatacc tggggcggtg gtttttccaa atattgtctg gttcctggtg
     6481 aaattctcaa aattcatcgt catgcgttgt gggaaatccc tgatggtgtt gattatgagg
     6541 acgcagccgt acttgaccct atctgtaatg cctataaatc catcgcgcag caatcgaaat
     6601 tccttcctgg tcaggatgtt gtcgtcattg gcactggccc actcgggctg ttttccgtac
     6661 aaatggcgcg gattatgggg gcggtaaata tcgtcgtcgt tggtctgcaa gaagatgtgg
     6721 cggtccgctt cccggttgca aaagaactgg gggcgacggc agtagtaaat ggttctaccg
     6781 aagatgtggt ggcgcgctgc cagcaaattt gtggcaaaga taatctggga ctggtgattg
     6841 aatgctccgg tgccaatatc gcactgaaac aagccatcga aatgctccgc ccgaatgggg
     6901 aagtggtacg cgttggaatg ggcttcaaac ctcttgattt ctcgattaat gacattaccg
     6961 cctggaataa aagcatcatt gggcatatgg cctatgactc cacctcatgg cgtaacgcta
     7021 tccggctatt agccagcggc gctatcaaag tcaaaccgat gatcacgcat cgtatcggcc
     7081 tgtcgcaatg gcgcgaaggg tttgatgcga tggtcgataa aaccgcaatc aaagtgatca
     7141 tgacttacga ctttgatgaa taactactct cctgaaggaa cgttatcgca tggaacagat
     7201 aacaaaaccg cattgtggtg cacggctgga tcgcttaccg gattgccgct ggcattcgtc
     7261 aatgtttgcc atcgtcgcgt ttggcttgct tgtctgctgg agtaatgccg ttggtggctt
     7321 gatcctcgcg cagctgaaag cgttgggctg gacagataat tccactacag ccacattctc
     7381 agcaatcacg accgccggaa tgtttctcgg tgcgctggtg ggcggcatca ttggcgacaa
     7441 aaccggtcgc agaaatgcgt tcatcctcta tgaggccatt catattgcct cgatggttgt
     7501 cggtgctttc tcaccgaata tggattttct tattgcctgc cgttttgtga tggacgttgg
     7561 gctgggagct ttactggtta cactgtttgc tggtttcacc gaatatatgc ccggtagaaa
     7621 tcgcggaacg tggtccagtc gggtttcctt tattggcaac tggtcatatc cgctctgctc
     7681 actgattgcg atgggactca cgccgctgat tagtgcagag tggaactggc gggtacaact
     7741 gcttattcct gcaatattgt cgcttatcgc tacggcgctg gcctggcgct actttcctga
     7801 atccccgcgc tggctggaat cgcgcggacg gtatcaggaa gccgagaaag tcatgcggag
     7861 tatagaagaa ggcgtcatac gccagacggg taaacctttg ccgcctgtgg ttattgctga
     7921 tgacggtaaa gcgccacaag cggtgccgta ttcagcctta ctgacaggag tattactgaa
     7981 acgcgtgata ttaggctctt gtgtgctgat taccatgaac gttgtgcagt acacactaat
     8041 taactggttg ccaacaatat tcatgaccca ggggattaat ttaaaagact cgattgtttt
     8101 aaataccatg agtatgtttg gcgcgccatt tggtattttt attgccatgc tggtgatgga
     8161 taaaattccg cgtaaaacaa tgggtgtggg gctattaatc ctgattgcgg tgctcggata
     8221 tatctattca ctgcaaacca gtatgttgct cataacgctg attggtttct tcctgattac
     8281 tttcgtctat atgtacgttt gctatgcctc ggcagtgtat gtccctgaaa tctggccgac
     8341 agaggccaaa ctccgtggct ccggtctggc gaatgcggta gggcgaatca gtggtattgc
     8401 cgcaccttat gccgttgcag tgctgctcag tagttatggt gtaacgggag tctttattct
     8461 tctgggggct gtttcaaata ttgtcgcaat tgctatcgcc accattggaa ttgaaaccaa
     8521 aggtgtctcc gttgaaagtt taagtattga tgcagtagcc aataaataat acgggtggat
     8581 tcaaaatgaa aaattcaaaa gcaatattgc aggtgccggg cacaatgaaa attatttcag
     8641 cagaaatacc agtgcctaaa gaagatgaag ttttgattaa agtagaatat gtcggtattt
     8701 gtggttcaga tgtacatggt tttgaatcag gcccgtttat tccgcctaaa gacccaaatc
     8761 aagaaattgg cctgggtcat gaatgcgccg ggacggttgt ggctgtggga agccgcgtgc
     8821 gcaaatttaa accgggggat cgggtaaata tcgaacctgg cgttccttgc ggtcactgtc
     8881 gttactgtct ggaaggcaaa tataacatct gcccggacgt tgattttatg gcgacacaac
     8941 ccaactaccg cggcgcatta acgcactatc tgtgtcatcc ggagagcttt acttacaaac
     9001 tgcccgacaa tatggacacg atggaagggg cgctggtgga gcctgccgca gtcgggatgc
     9061 atgccgcgat gctggcagat gttaaaccgg gtaagaagat aattattctg ggagcaggtt
     9121 gtattggttt gatgacgttg caagcgtgca aatgcctggg cgcaacggaa attgccgtcg
     9181 ttgatgtact ggaaaaacgt ctggcaatag gggaacagcc tggtgcgaca gtggttatta
     9241 atggcgcaaa agaggacact attgcacgct gtcagcaatt taccgaagac atgggcgcag
     9301 atattgtttt cgaaacagcg ggttctgcgg tcaccgttaa acaggcacct ttaatctggt
     9361 aatgcgcggc ggtaaaatta tgattgttgg tactgtaccc ggcgattcgg caatcaattt
     9421 cctcaaaatc aatcgcgaag tcactatcca gacggtattc cgctatgcca atcgttatcc
     9481 ggtcacgatt gaagctattt cttcagggcg attcgatgtg aaatcgatgg tgacgcatat
     9541 ttacgattat cgggatgtac aacaggcatt tgaagagtca gttaacaaca aacgcgacat
     9601 tattaaaggc gttattaaaa ttagcgatta attaatacgg gagaacagaa catgctcgca
     9661 gatatcagat attgggaaaa cgatgccaca aataaatatt atgcaattgc ccattttaac
     9721 gtatggaatg cagaaatgtt gatgggcgtt aaagacgcag ccgaagaagc gaaatccccg
     9781 gttattattt cttttagtac aggttttgtc ggtaacacct catttgaaga tttctctcac
     9841 atgatggtat caatggcaca aaaagcaacg gtgccggtaa taactcattg ggatcatggt
     9901 cggagtattg agattattca taacgcctgg actcatggta tgaattcatt aatgcgtgat
     9961 gcttctgcat ttgatttcga agaaaatatt cgtttaacca aagaggctgt tgatttcttt
    10021 catccgctgg gtattccggt agaggcggaa ttagggcatg tcggtaatga aaccgtttat
    10081 gaggaagcgc tggcgggcta tcactatacc gatcctgacc aggcggctga atttgttgaa
    10141 cgtacgggct gtgactcatt ggctgtcgcc atcggcaacc agcatggggt ttatacgtca
    10201 gagccgcaat tgaactttga ggtcgtcaaa cgcgtacgcg atgctgtttc tgttccgctg
    10261 gttttgcatg gcgcatcggg gatcagtgat gccgacatta aaactgcaat ttcgttgggt
    10321 attgcgaaaa tcaacattca tacggagctc tgtcaggccg caatggtagc agtgaaagaa
    10381 aaccaggatc agcctttcct gcatttagaa cgtgaagtac gtaaagctgt aaaagaacgg
    10441 gcattggaaa aaattgaact gttcggttct gatggcaaag cggaataatg atatggataa
    10501 tctcgacgtt atttgtatag gtgccgctat tgttgatatt ccattacaac cggtcagtaa
    10561 aaatatcttt gatgtagatt cttatcctct tgaaagaatc gcaatgagca ccgggggcga
    10621 tgcaataaat gaagcaacaa ttatttctcg tctggggcat cgtaccgcat taatgagccg
    10681 cattggtaaa gatgcctcag ggcaatttat tctcgaccat tgtcgcaaag agaatattga
    10741 tattcaaagc ctgaaacagg acgtcagcat agatacatct attaacgttg gactggtgac
    10801 ggaggatggc gagcggacgt ttgtcacgaa ccgtaacggc agtttgtgga aattaaatat
    10861 tgacgatgtt gattttgcgc ggtttcctca ggcgaaatta ttatcactgg ccagtatttt
    10921 caacagtcct ctattggatg gtaaagcgct aacagaaatt tttacgcaag cgaaagcccg
    10981 gcagatgatc atttgtgccg atatgatcaa accgcggttg aatgaaacgc tggatgatat
    11041 ttgcgaagca ttgagttatg tcgattatct gtttccaaat tttgccgagg aaaaattact
    11101 cacagggaaa gagacactgg atgaaattgc tgactgcttt cttgcgtgcg gcgtaaaaac
    11161 ggtggtgatt aaaacgggta aagacggctg ctttatcaag cgtggtgaca tgacgatgaa
    11221 ggtgccggcg gtcgcaggaa taaccgccat cgacaccatt ggcgcgggcg ataactttgc
    11281 ttcaggtttt attgcggcac tgttagaagg caaaaatctg cgtgaatgcg cacgctttgc
    11341 caatgcaacg gcggctatct cggttctaag cgtcggtgcc accaccggcg taaaaaacag
    11401 aaagctggtc gaacaattgc tggaagaata cgaaggataa tgaaggcaaa tgaaaaagat
    11461 acctttaggc acgacggata taacgctttc gcgaatgggg ttggggacat gggccattgg
    11521 cggcggtcct gcatggaatg gcgatctcga tcggcaaata tgtattgata cgattcttga
    11581 agcccatcgt tgtggcatta atctgattga tactgcgcta ggatataact ttggcaatag
    11641 tgaagttatc gtcggtcagg cgttaaaaaa actgccccgt gaacaggttg tagtagaaac
    11701 caaatgcggc attgtctggg aacgaaaagg aagtttattc aacaaagttg gcgatcggca
    11761 gttgtataaa aacctttccc cggaatctat ccgcgaagag gtagaagcca gcttgcaacg
    11821 tctgggtatt gattacatcg atatctatat gacgcactgg cagtcggtgc cgccattttt
    11881 tacgccgata gctgaaactg tcgcagtgct taatgagtta aaggctgaag ggaaaattcg
    11941 cgctataggc gctgctaacg tcgatgctga ccatatccgc gagtatctgc aatatggtga
    12001 actggatatt attcaggcga aatacagtat tctcgaccgg gcaatggaaa acgaactgct
    12061 gccgctatgt cgtgataatg gcattgtggt tcaggtttat tccccgctag agcagggatt
    12121 gttgaccggc actatcactc gtgattacgt tccgggcggc gctcgggcaa ataaagtctg
    12181 gttccagcgt gaaaacatgc tgaaagtgat tgatatgctt gaacagtggc agccactttg
    12241 tgctcgttat cagtgcacaa ttcccactct ggcactggcg tggatattaa aacagagtga
    12301 tttaatctcc attcttagtg gggctactgc accggaacag gtacgcgaaa atgtcgcggc
    12361 actgaatatc aacttatcgg atgcagacgc aacattgatg agggaaatgg cagaggccct
    12421 ggagcgttaa atactcaaca cgattcacct ctgtttctgt gaaaactgtg atgaaaatct
    12481 gtgattcaaa taggttattt tgaaagtaaa catcggttat gcgataatcg cgctaatgtg
    12541 atgtaaaaag atacaggggt gtaatcgtgg cggcaaaaga caggattcag gcaattaagc
    12601 aaatggttgc caacgataaa aaggtgacag tctcaaattt gagtgggatt tttcaggtaa
    12661 ccgaagaaac cattcgccgc gatcttgaga agctggaaga tgaaggcttt ttgaccagaa
    12721 cctatggtgg tgctgtttta aatacagcgg tgttgacgga gaatatccat ttttataagc
    12781 gcgcttcatc gttttatgaa gagaagctgc ttattgcacg caaggcacta ccctttatcg
    12841 acaataaaac caccatggca gccgattcca gtagtaccgt aatggaattg ctcaaattgt
    12901 tacaggaccg tagtgacctg acgttgctaa ccaactcggc ggaagcgatt catgttctgg
    12961 ctcagtcaga aattaaagtc gtttcaacgg gtggggaact aaacaaaaat acactttccc
    13021 tgcaaggaag aattactaaa gagatcatca gccgctacca tgtcgatatc atggtaatga
    13081 gttgtaaagg tcttgatatt aacagcggcg cgctggactc taatgaagcg gaagctgaaa
    13141 tcaaaaagac aatgatccgt caggcgacag aagttgcgtt attggttgat cactctaagt
    13201 ttgatcgtaa agcttttgtc cagttagctg actttagtca tattaattat ataataactg
    13261 ataaatcacc gggtgcagag tggatagcat tttgcaaaga caataatatc cagctggtgt
    13321 ggtaatttaa gcacacgttg tcaatattat ttgttcctgc gactggaaat aaaatgatga
    13381 taaagatgcg gcaagtgaat ttgccgcaat tgctaccttt tatagcaaat ccgaggcacc
    13441 caatggaaca atatgatcaa attggcgcaa gactggaccg cttgcctttg gcccggtttc
    13501 attatcgtat atttggtata ataagcttta gtctgttatt aacggggttt ttgagttact
    13561 caggtaatgt cgtcttagca aagctggtaa gcaatggatg gtcaaataat ttcctctatg
    13621 ccgcctttac ctcggcatta atgtttggtt atttcatcgg ctcacttact ggtgggttta
    13681 ttggtgacta ctttgggcgg cgcagggcgt ttcgcataaa tcttctcatc gtcggtattg
    13741 ctgcaacagg ggccgctttt gtccctgata tgtactggct cattttcttt cgcttcctga
    13801 tgggaacagg aatgggggcg ctgattatgg ttggctatgc ctcatttacg gagtttatcc
    13861 ccgcgacggt gcgtggaaaa tggtccgcgc ggctctcatt tgttggtaac tggtcgccca
    13921 tgctgtctgc ggcgataggc gtggtggtta tcgctttttt tagttggcga ataatgtttc
    13981 ttctgggtgg tattggcata ctgttagcct ggtttctctc aggtaaatac tttatcgagt
    14041 cgccacgatg gctggaaggg aaagggcaaa tcgccggtgc agaaagccaa cttcgtgaag
    14101 tagagcagca aattgaaagg gagaagagta ttcgtttacc ccagcttact ttgaaccaga
    14161 gcaacagcaa ggttaaggta atcaagggta ccttcaggct cctgtttaaa ggggaaatgt
    14221 tacgacgtac attagttgcg ataactgttt taattgcaat gaacatttcg ctttatacca
    14281 ttaccgtatg gataccgacc atatttgtta actccggcat tgatgtcgat aaatcaatat
    14341 taatgaccgc tgttattatg attggtgctc cggtaggaat atttattgcg gcattaatta
    14401 ttgatcattt tcctcgtcga ttatttggct ccgccttact tattattatt gccgtgttag
    14461 gctatatcta ttcaagtcag actacagagt gggcgatttt aatctatggt ctggtgatga
    14521 tcttcttttt atacatgtat gtttgcttcg cgtcggcggt ttatatcccg gagctttggc
    14581 caacgcattt acgcctgcgc ggttcgggtt tcgttaatgc cgtcggacgg atcgtcgcag
    14641 ttttcacgcc ctatggcgtt gcggcattat taacacatta tgggtcgatc acggtgttta
    14701 tggtgcttgg tgtcatgtta gtgctctgtg cgctggttct ctccattttt ggcatcgaaa
    14761 cgcggaaggt gtcgctggaa gagatttctg aggtgaatta aatctttcgt tgatggcatc
    14821 aattgccaga tgtaccactg acgtgtttta tttggcctac aatgccagta cagaattgta
    14881 ggccagcgta aaattacccc tgcgtctctt cccagtctgc cagcgtatat agcgttgccc
    14941 cagctgctga catctccata aacgcgtgtg cactgtcctg gggctggata ttcacgccac
    15001 gacagccatc ggtaatcacg tttaccttat aacctaactg taacgcgtcc agcacggtaa
    15061 acttcacgca atagtcagta gccaggccca taacgatcaa ttcatcgatt tcatgatcgc
    15121 gcaaccagtc atcaagcgcg gttttctgcc gacgtccgtt atcaaaaaag gcactgtaac
    15181 tgtcaactaa aggattttcg cctttatgga acaccgctgc gatctctttt tgattcagta
    15241 acggatgtaa ttgtgcgcct tcactgttct gcacacagtg ttcaggccag aaagtttgcc
    15301 gcaggccgcc gagttggcct ggcgtataag gctctacgcc gtgctggctg gcaaagctgc
    15361 cgtgattcgc cggatgccag tcctggctgg cgataaccgc ttctccgcgc gactggcacc
    15421 agtcaataag gcggttagca acctccaccg tactgtcacc ttccggtacg gcgagcgcgc
    15481 caccagcaca gaaatcattt tgtaaatcga ccagtaacag ggcgcgatgg ggcattgcag
    15541 tctccttaat catccggcgt cagttcgccg cgcaggtttt ggctcatggc cttgcgaatg
    15601 gtttcagtat ccagttcctg gctcagcagg taatgcagtt tggttagcgt ggcttctaca
    15661 gtcatatctg caccgtcaat aacgccggca tgggcgaggg cgttaccggt ggcataacca
    15721 ctcatattca ctttaccgga catacattgt gtcaggttga ccaccacaat accgcgatcg
    15781 ctggcttctt gtaattcctg caggaaggct ttgttttgtg gcgcattacc cacgccatag
    15841 gagcgcagaa tcaatgcttt caccggttgg cgcagaaaat tgcgcacgac gtcagcagaa
    15901 atccctggat aaatcgtcac cacgccaatt ggttgtggcg tgattggatg aacgattaat
    15961 tccccttcac cgtgcggggc gggtggcgta ttcaaacgac gaatatggat accagcttcc
    16021 agtaacggag gaaggtttgg agaggcaaac gcatcaaaac catcggcatg ggctttggta
    16081 gtgcggttgc cgcgatacaa tcggttattg aaaaagagcg ttacttcgtt gatcggataa
    16141 ttcggcgcaa cgtacaacgc attcagcaga ttaatttgtc cgtcagagcg taactcagcc
    16201 agcgggattt gtgaccctgt cacaatgacc ggtttaccga gattctcgag catgaacgac
    16261 agcgcagagg cggtatacgc catcgtgtcg gtaccgtgta gaatgacaaa accatcatag
    16321 tcgtcatagt gcgctttaat atcttcagca atatgctgcc agtcttccgg cgtcatatca
    16381 gaagaatcca tcaacggcgt atattcatga atggtgaaat ctggcatctc cgggcgatgg
    16441 aattccggca tcagcgccag ttggcgttgt agatgacctg acaccggaat ataaccctgc
    16501 tcggaacgct gcatcccgat ggtcccgccc gtgtaggcga cgtaaattga tttcttttgc
    16561 atgatattga tatgaatgat atcgaaagag caaaagtata tacgtgaggc aggaggagcg
    16621 cacctgatac tatgcggata aggaaatatg gggcgagaca gcgggaagag gattcgtcga
    16681 ggtaaaaaat aatgtggccg acgatctgtc ggccactcag tacaagactt aacgcacgtt
    16741 ggcgcaggtc aggcaaaacg cataacggtt ttgtgggtcg ttaaacgcgg ccagcttgtc
    16801 gctttcactt ttaacggtag aggctaccga ggcaagcggt gcaggtaaca tggcctggaa
    16861 cgcatctggc aacattgccc ggacagaacc agacatgttg tccatcactt tgtcgaagaa
    16921 ggtcggttca tcaacgtagt attccagatg ccactgtttc acttttgcga gctctgctgc
    16981 tttggcaacc gcatcatcga aatccccgag actatcgacc agcccgttag cttttgcatc
    17041 ctgaccggtc cagacgtggc cctgggcgat tttatcaatt tgctccggcg tcgaatgacg
    17101 cgcatcagca accagcgtga taaagcgttt atagccattc tcaatgctta attgcatcat
    17161 ctgctgcgct tccggcggca gtgccctggt gatagaaaca tccgccagcg gtgaagttga
    17221 gacaccatca gtatgaacac caatcgaatc cagactattt tctacggtgg tgatcacgcc
    17281 gaagataccg atagaaccgg tcagggtgct ggggttagcc acaatgtaat tagctggcgt
    17341 ggaaatccag taaccaccag atgccgccat gccgcccatc gatacaacca cagacttacc
    17401 cgctgcccgg gctgctgcca gttcagcgcg aatcacttca gacgcggtaa cgctgccgcc
    17461 tgggctatta acacgcagga caatcgcttt cactttcggg tcaaggcgag cgtcgcggat
    17521 ttgtgccgca gtggtatcac cgccaacatt cccctgagtt tcctcgccat ccataattgc
    17581 gccattagcg aagacgacac cgatgctgtc accggtatct gccggcgttt tcaatgcgta
    17641 atcgtaataa ctgatggcgc gataattttt atcagtctta ctccagccga actctttggt
    17701 cagagctttt tcgatttcgg cactcgatgc cagtgcatcg accagcttgt tttccagtgc
    17761 atatttcgcg gtatcgccac cggttttggt taaaccctca agcaaccctt gcgcgccagg
    17821 gaatacctgc tgagcaggga tctgccggtt agcggcaaca gtattcagat agttttgcca
    17881 cagctcacca atccagcggc tgtcagcttc gcgggctgcc ggtgacatat catcacgaat
    17941 aaacggttca acggcagatt tatacgtacc cacgcggaac acatgggtgg aaactttcag
    18001 cttatccagc aacgatttgt agtacagacc gttggtagca aaaccgtgca gatcaaccac
    18061 gccttgcggt gacagccaaa ttttattggc gaaactggcg agataatatt gcccctggct
    18121 gtagttctcg ccaacggcat aaaccggttt cccgctgtca cgaaactctt tcagggcttt
    18181 gccgatgtac tgcatagacg gctggtcgcc gcctgcgaag tttttcagat ccatcacgat
    18241 accggtgata ttgcggtcgt ccttcgcctg gcgaatggtg ttgacgatat caaacagtga
    18301 gttttcctgc agacgatcgg aactggcacc aagcagctgg cggcttaatt tactaaaccg
    18361 ctgagaactg tctggtttat cgacgatcac accagaaatg tccagcagca gtgcgccacg
    18421 actggccgtt tctttcgaat caccaccact gacctgcatc cagatcccca caccaaccag
    18481 tacgaggaaa ataaagaaca ggttaagtac catttcacgg acgaaattca gcagacgcca
    18541 cgtccattta aaaaatccgg caataaatcg ccaaagggtt cgcatgtatt ctcccaactt
    18601 aaggtcacac ctgtcacagg cgcaatagat gtggttatcg taatgaccca accggcactt
    18661 gtcagcagga attgcggact gtgctgtaac aaaatccact ctcgtgttaa ttttgtgaat
    18721 aaatatcacg acaggagtta atcaaatgga tgcactcgaa ctattgatca atcgccgtag
    18781 cgcctcccgc ctggctgaac ccgcgccaac gggtgaacaa ctgcaaaaca tcctgcgtgc
    18841 gggtatgcgt gcgccggacc ataagtccat gcaaccgtgg catttttttg tgattgaagg
    18901 ggaagggtgc gagcgtttca gcgccgtact ggaacagggg gcgattgctg ccggtagtga
    18961 tgataaagct atcgacaaag cccgtaatgc gccgttccgc gcaccgctca tcatcacggt
    19021 ggtggcgaaa tgcaaagaaa atcataaagt cccgcgatgg gaacaggaaa tgtctgccgg
    19081 atgcgcggtc atggcgatgc aaatggcagc cgttgcccag ggatttggcg gcatctggcg
    19141 cagtggcgca ttaactgaaa gtccggtagt gcgtgaagca ttcggttgcc gtgagcagga
    19201 taaaattgtc ggttttctct acctcggcac gccgcagttg aaagcatcta cgtcgattaa
    19261 cgtcccggac ccgacgccgt ttgtaactta tttctgataa ttgtcgcgaa actgtctgga
    19321 ttgtgagcaa aggcgcagga attcagacac tctcacttat cacttcacgg aatgagggtt
    19381 accatacccg gattgcattg acaggagatg tccatgagcg agaactcgat tcgtttgacc
    19441 caatacagcc acggagctgg ttgcggctgt aaaatttccc caaaagtgtt ggaaaccatc
    19501 ctgcacagtg agcaggcgaa gtttgttgat ccgaatttgc ttgtgggtaa tgaaacccgc
    19561 gacgatgcgg cggtgtacga tctgggcaat ggcaccagcg ttatcagtac caccgacttc
    19621 tttatgccga tcgttgataa tcctttcgat tttggccgca ttgcggcgac taacgccatc
    19681 agcgatatct tcgcgatggg gggcaaaccg attatggcga ttgcgatcct cggctggccg
    19741 attaacaaac tttccccgga aattgcccgc gaagtaaccg aaggtggacg ctatgcatgc
    19801 cgtcaggcgg gtattgcgct ggctggcggt cactccatcg atgcgccgga gccgattttt
    19861 ggtctggcgg taacggggat cgtaccgacc gagcgggtga agaaaaacag taccgcacaa
    19921 gccggatgca aactgttcct gacgaaaccg ctggggatcg gcgttcttac cacggctgag
    19981 aaaaaatcac tgttgaaacc agaacatcag ggactggcga cggaagtgat gtgccggatg
    20041 aacatcgcag gcgcgtcctt tgccaacatc gaaggcgtaa aagcgatgac cgacgttacg
    20101 ggctttggtc tgctgggcca cttgagcgaa atgtgtcagg gggctggtgt gcaggcacgc
    20161 gtcgactatg aagcgatccc gaaactcccc ggtgttgaag agtacattaa gttgggcgca
    20221 gtacctggcg gcactgaacg taactttgcc agctacggtc atctgatggg tgaaatgccg
    20281 cgtgaagtgc gcgatctgct gtgcgatccg caaacttctg gcggtttgct gctggcggtc
    20341 atgccggaag cagaaaatga ggttaaaact acagccgccg agtttggcat tgaactgacg
    20401 gcgattggcg aactggtgcc agcgcgcggc ggtcgtgcca tggttgagat ccgttaattt
    20461 aatgcggttg tttattgccg aaaaaccgag tctggcgcgc gccattgctg atgtcctgcc
    20521 caaaccgcac cggaaaggcg atggctttat cgagtgcggt aatggtcagg tggtgacttg
    20581 gtgtatcggt cacctgcttg agcaggcgca gccagacgcc tacgacagcc gctatgcgcg
    20641 ctggaatctt gcggatttgc cgattgtccc ggaaaagtgg caattacagc cacgaccctc
    20701 cgtgaccaaa caacttaacg tcatcaaacg cttcctgcat gaagccagcg aaatcgttca
    20761 cgccggggac ccggatcgtg aagggcaatt gctggtggat gaagtgctgg actatctgca
    20821 actggcaccg gaaaagcgcc agcaggtgca gcgttgcttg ataaacgacc tgaacccgca
    20881 ggcggttgag cgggcgatcg accgtcttcg ttccaacagt gagtttgtgc cattgtgcgt
    20941 ttctgcgctg gcgcgagcgc gtgccgactg gctgtacggc atcaatatga cccgtgcgta
    21001 taccattctc ggtcgcaatg ccggttatca gggcgtactt tccgtgggac gcgtgcagac
    21061 acccgtgctc gggctggtgg tgcgccgcga tgaagagatt gaaaacttcg tggcgaaaga
    21121 cttctttgaa gtcaaagcac atatcgtgac acctgccgat gagcggttta ccgctatctg
    21181 gcaaccgagc gaagcgtgtg aaccgtacca ggatgaagaa gggcgcttgt tacatcgtcc
    21241 actggcggag catgtggtta accgcattag tggtcaaccg gctattgtca ccagctataa
    21301 cgataaacgg gaatcagaat ccgcgccgct gccgttttcg ctttcggcgt tgcagattga
    21361 agcggcaaaa cgcttcggtc tgagcgcgca gaacgtgctt gatatctgcc agaagctgta
    21421 cgaaacgcac aagctaatca cttatccgcg ttccgattgt cgctatttgc cagaagaaca
    21481 ttttgccgga cgccacgcgg tgatgaatgc catcagcgtt catgcaccgg atctgttgcc
    21541 gcagccagtg gtagatccag atatacgcaa ccgctgttgg gatgacaaaa aggtcgatgc
    21601 gcaccacgcc atcatcccga ccgcacggag ttctgcgatc aacctgacgg agaacgaagc
    21661 gaaggtctat aacctgattg cccgtcaata cctgatgcag ttctgcccgg atgcggtgtt
    21721 ccgcaagtgt gttatcgaac tggacattgc caaaggcaaa tttgtcgcta aagcccgttt
    21781 tcttgctgaa gcaggctggc gcacgctgtt aggcagcaaa gagcgcgatg aagaaaacga
    21841 cggcacgcca ttgcctgtgg tggcgaaagg cgatgagttg ctgtgtgaaa aaggtgaagt
    21901 ggtagagcgg caaacccagc cgccgcgcca ttttaccgat gcaacactgc tttcggcgat
    21961 gaccgggatc gcgcgctttg tgcaggacaa agatctgaaa aaggtccttc gtgcgaccga
    22021 tggtctgggg acagaagcaa cccgtgccgg gattattgaa ctgctgttca agcgtggttt
    22081 tctgaccaaa aaagggcgct atatccactc caccgacgcc ggaaaagcgt tattccattc
    22141 gctgccagaa atggcgacgc gaccggacat gaccgcgcac tgggaatcgg tgctgacgca
    22201 aatcagcgaa aagcagtgtc gctatcagga ctttatgcag ccgctggtgg ggacgctata
    22261 tcagcttatt gatcaagcca aacgtacgcc ggtgcggcag tttcgcggca ttgtggctcc
    22321 gggcagtggt ggcagtgctg ataagaaaaa ggctgcaccg cgtaaacgta gtgcgaaaaa
    22381 aagtccgcca gcagatgaag ccggaagcgg ggcgatagcg taagcgagcg aatctttcgt
    22441 gctattcgag tcatattctg aaatatccag cggatcaaga aagttcgttg gatatttttt
    22501 tgcatggata aaattatcgc ctctaaagta tgtaataaca gggaatgtga tgaaaaaagt
    22561 tttattgcaa aaccatcctg ggagcgagaa gtattctttt aatggctggg gaatatttaa
    22621 tagtaatttt gaacgggtga ttaaagaaaa taaggccatg ctgctttgta agtgggggtt
    22681 ttatttaaca tgtgttgtcg ctgtaatgtt tgtattcgca gcgataacat ccaacggttt
    22741 gaatgaaaga ggcctgatta ccgcgggatg ctcttttctt tatctattaa ttatgatggg
    22801 gcttattgtt cgggccggtt ttaaagcaaa aaaagaacaa ctgcattatt atcaggctaa
    22861 aggtattgag ccgctcagta tcgagaagtt acaggcgcta caattgatcg caccttatcg
    22921 attctatcat aagcaatggt ctgaaacgct agagttctgg ccgcgaaagc ctgaacctgg
    22981 caaagatacc ttccaatatc atgtgcttcc ttttgactcg atcgatatca tcagtaaaag
    23041 acgcgagtct ttagagaatc aatggggtat cgaagatagc gaaagttatt gtgccttaat
    23101 ggagcatttt ctttctggcg accatggagc caataccttt aaagcaaaca tggaggaagc
    23161 cccagagcag gttatcgcct tgttgaataa atttgctgtt tttccctcag actatatctc
    23221 tgattgcgct aataatcgct ccggtaaatc ctcggcgaag ctaatatggg cggcggaatt
    23281 atcatggatg atctcgatat caagcacagc ttttcaaaac gggacaattg aagaagaact
    23341 ggcctggcat tatataatgc ttgcttctcg aaaggcgcac gagttgttcg aaagcgaaga
    23401 agattatcaa aaaaatagtc taatgggatt tctttactgg catatctgct gctatcgcag
    23461 aaagttaacg gatgctgaac ttgaagcgtg ttatcgttac gacaagcagt tttgggagca
    23521 ctacagtaag aaatgccgtt ggcccataag aaatgttccc tggggagcat catccgttaa
    23581 atactcataa acgcctgaaa ttttgccggg ggcgctgcgc ttgaccggcc tacattagag
    23641 cgtaactgca atgaattgtg cccatctgta ggcctgataa gcgtagcgcc atcaggcatt
    23701 tacaacttaa atcacaccct gcgccagcat cgcatcggca accttcacaa aaccggcaat
    23761 gttcgcgccc tgcacgtagt tggtttgctc accttcacca ccatgctcaa cacaggcatg
    23821 gtggatatcc agcatgatgt gatgcaaacg tgcgtcaact ttctcggctt tccagcccag
    23881 gcgcgcagcg ttttgtgcca tttccaggcc cgacgttgct acaccgccag cattagccgc
    23941 tttacccggt gcaaacagta cgcctgcctg ctggaacagt tcagtcgctt cgatggtggt
    24001 cggcatatat gccccttcag cgacggcttt aacgccattg gcgataagct gatgcgcggc
    24061 gtcaacatcc agttcgttct gggtggcgca aggcagggcg atatcaaccg gtacagacca
    24121 cggctgttgg ccttcgagat agaccagacc aaattctttg gcgtaatctg ccgctcgacc
    24181 atcgcggctg gctttgattt cgataagacg tgccagtttc tctttcgtga atccgctttc
    24241 atcaactaca gtgccgctgg agtctgacgc agtgatcaca cgagcaccaa attccatcgc
    24301 tttttcgata gcgtactggg cgacgttgcc ggagccagaa acggaaacgc tcatcccttc
    24361 aaaacccata ccgtggcgtt ttaacattgc ttctgtgaaa taaaccagac cgtagccggt
    24421 agcttccggg cgaataagac taccgccaaa tgaaaggccc ttaccggtga agacgcaggc
    24481 ggtattgttg gagagctttt tcatcatccc cgccataaag ccgacttcac gaccaccaac
    24541 gccgatatca cctgccggaa cgtcggtatc cgcgcccagg tggcgataca gttcagtcat
    24601 cagcgtctgg caaaaacgca tcactttacc ttcgcttttt cctttcggat cgaaatcgct
    24661 gccgccttta ccaccgccca tcggcagggt agtcagggca tttttgaagg tttgttcaaa
    24721 gccgaggaat ttgagaatgg aaaggttaac tgacggatgg aagcgcatac cgcctttgta
    24781 cgggccgatg gcagagctga actgcacacg ccatgcacgg ttgacctgta tctggttgcg
    24841 atcatcaacc cataccacgc gaaactggat cacgcgctcc ggttcaacca ga
//
LOCUS       DAJVQR010000057        23934 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVQR010000057 DAJVQR010000000
VERSION     DAJVQR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(1..386)
                     /locus_tag="OQM52_002446"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OQM52_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OQM52_002447"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OQM52_002447"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1564260.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OQM52_002448"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OQM52_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1564261.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OQM52_002449"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OQM52_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1564262.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OQM52_002450"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OQM52_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1564263.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OQM52_002451"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OQM52_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1564264.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OQM52_002452"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OQM52_002452"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1564265.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OQM52_002453"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OQM52_002453"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1564266.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OQM52_002454"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OQM52_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1564267.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OQM52_002455"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OQM52_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1564268.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OQM52_002456"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OQM52_002456"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1564269.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OQM52_002457"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OQM52_002457"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1564270.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OQM52_002458"
     CDS             10748..11212
                     /locus_tag="OQM52_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1564271.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OQM52_002459"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OQM52_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1564272.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OQM52_002460"
     CDS             12236..13672
                     /locus_tag="OQM52_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1564273.1"
                     /translation="MPLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OQM52_002461"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OQM52_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1564274.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OQM52_002462"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OQM52_002462"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1564275.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OQM52_002463"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OQM52_002463"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1564276.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OQM52_002464"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OQM52_002464"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1564277.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OQM52_002465"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OQM52_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCS1564278.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OQM52_002466"
     CDS             complement(20505..20798)
                     /locus_tag="OQM52_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1564279.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OQM52_002467"
     CDS             complement(20795..22084)
                     /locus_tag="OQM52_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1564280.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OQM52_002468"
     CDS             complement(22140..23078)
                     /locus_tag="OQM52_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCS1564281.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OQM52_002469"
     CDS             complement(23098..23862)
                     /locus_tag="OQM52_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCS1564282.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5911 a         5805 c         6301 g         5917 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga actgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgcc
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgat
//
LOCUS       DAJVQR010000058        23851 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVQR010000058 DAJVQR010000000
VERSION     DAJVQR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(12..584)
                     /locus_tag="OQM52_002470"
     CDS             complement(12..584)
                     /locus_tag="OQM52_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS1564283.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            802..1662
                     /gene="tesB"
                     /locus_tag="OQM52_002471"
     CDS             802..1662
                     /gene="tesB"
                     /locus_tag="OQM52_002471"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1564284.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1711..2997)
                     /gene="amtB"
                     /locus_tag="OQM52_002472"
     CDS             complement(1711..2997)
                     /gene="amtB"
                     /locus_tag="OQM52_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1564285.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3027..3365)
                     /gene="glnK"
                     /locus_tag="OQM52_002473"
     CDS             complement(3027..3365)
                     /gene="glnK"
                     /locus_tag="OQM52_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1564286.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3546..5327)
                     /locus_tag="OQM52_002474"
     CDS             complement(3546..5327)
                     /locus_tag="OQM52_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1564287.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5320..7092)
                     /locus_tag="OQM52_002475"
     CDS             complement(5320..7092)
                     /locus_tag="OQM52_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1564288.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7122..7580)
                     /gene="decR"
                     /locus_tag="OQM52_002476"
     CDS             complement(7122..7580)
                     /gene="decR"
                     /locus_tag="OQM52_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1564289.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7734..8552)
                     /gene="cof"
                     /locus_tag="OQM52_002477"
     CDS             complement(7734..8552)
                     /gene="cof"
                     /locus_tag="OQM52_002477"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1564290.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8652..10344
                     /locus_tag="OQM52_002478"
                     /pseudo
     CDS             8652..10344
                     /locus_tag="OQM52_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10409..11104
                     /gene="queC"
                     /locus_tag="OQM52_002479"
     CDS             10409..11104
                     /gene="queC"
                     /locus_tag="OQM52_002479"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1564291.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11156..11554)
                     /gene="fadM"
                     /locus_tag="OQM52_002480"
     CDS             complement(11156..11554)
                     /gene="fadM"
                     /locus_tag="OQM52_002480"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1564292.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11648..12019)
                     /locus_tag="OQM52_002481"
     CDS             complement(11648..12019)
                     /locus_tag="OQM52_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1564293.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12170..14041)
                     /gene="ppiD"
                     /locus_tag="OQM52_002482"
     CDS             complement(12170..14041)
                     /gene="ppiD"
                     /locus_tag="OQM52_002482"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1564294.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14233..14505)
                     /gene="hupB"
                     /locus_tag="OQM52_002483"
     CDS             complement(14233..14505)
                     /gene="hupB"
                     /locus_tag="OQM52_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1564295.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14714..17068)
                     /gene="lon"
                     /locus_tag="OQM52_002484"
     CDS             complement(14714..17068)
                     /gene="lon"
                     /locus_tag="OQM52_002484"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1564296.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17256..18530)
                     /gene="clpX"
                     /locus_tag="OQM52_002485"
     CDS             complement(17256..18530)
                     /gene="clpX"
                     /locus_tag="OQM52_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1564297.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18656..19279)
                     /gene="clpP"
                     /locus_tag="OQM52_002486"
     CDS             complement(18656..19279)
                     /gene="clpP"
                     /locus_tag="OQM52_002486"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1564298.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19526..20824)
                     /gene="tig"
                     /locus_tag="OQM52_002487"
     CDS             complement(19526..20824)
                     /gene="tig"
                     /locus_tag="OQM52_002487"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1564299.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21168..21485)
                     /gene="bolA"
                     /locus_tag="OQM52_002488"
     CDS             complement(21168..21485)
                     /gene="bolA"
                     /locus_tag="OQM52_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1564300.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21790..22368
                     /locus_tag="OQM52_002489"
     CDS             21790..22368
                     /locus_tag="OQM52_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1564301.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22412..>23851
                     /gene="ampG"
                     /locus_tag="OQM52_002490"
     CDS             22412..>23851
                     /gene="ampG"
                     /locus_tag="OQM52_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1564302.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLL"
BASE COUNT         5578 a         6599 c         5775 g         5899 t
ORIGIN      
        1 gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt cacctgagtt
       61 ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg cacggcggtt
      121 tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat caccggctga
      181 acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact caacagaata
      241 cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa tggtgactgt
      301 ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc ggctaacgac
      361 gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt ctgacggatc
      421 cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc tgaaatagac
      481 gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca agccgccaac
      541 gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt ctccttatta
      601 tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa tagtaacgtc
      661 aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt cagactattt
      721 cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct tgctgcggca
      781 gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac attgttaaat
      841 ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg tttacgccag
      901 gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac cgtcccggaa
      961 gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag taagaaaccg
     1021 attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg ccgggttgct
     1081 gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc accagaagcg
     1141 ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct gccttcggaa
     1201 acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga taaattcatc
     1261 tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa aggtcacgtc
     1321 gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga tgacctgcgc
     1381 gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt agctctacag
     1441 ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga ccattccatg
     1501 tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga gagcacctcg
     1561 gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg cgtgctggtt
     1621 gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag ccggaggtga
     1681 aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct cgccgtggct
     1741 gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa ccgtcagatc
     1801 cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa tggcgatgct
     1861 ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc ccacgccgcc
     1921 cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac aaacgccgtg
     1981 cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca tggtaacgcc
     2041 ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa ccccaatgta
     2101 gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg ctcccagcag
     2161 agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg ccgccgccgt
     2221 tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc ccactgaccc
     2281 ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga ccatcggcag
     2341 gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca gatacgcgcc
     2401 caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga aatccagcgc
     2461 accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa tgtaagagag
     2521 cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt ccgccagcgc
     2581 cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta cgtggatata
     2641 ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca accagttaat
     2701 gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa ccacccagag
     2761 aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca cgtttttgcc
     2821 gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca gcaccagcgc
     2881 agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg cagccattac
     2941 cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta tcttcatttt
     3001 tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc gccggtacga
     3061 atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat ttttccggtg
     3121 taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc aatcgccaca
     3181 tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta cagctcggca
     3241 tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg aataccaatg
     3301 gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac ggtcaccagc
     3361 ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag aaggtattgc
     3421 aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa gattgaaaat
     3481 gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat gaaatattgc
     3541 gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc ttcgcccgca
     3601 agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg ctggtgagtg
     3661 ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc aataatagtt
     3721 gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc cagagcatgt
     3781 tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag gatcaggatt
     3841 tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc gactgagaga
     3901 ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg cgccagctcc
     3961 gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc ccgccccagc
     4021 gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac cattgccacg
     4081 ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag gtgaatctca
     4141 ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt accactgccg
     4201 gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat gttctttagc
     4261 accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt gccactctgt
     4321 aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc aaacacgcgc
     4381 tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc gattaagggt
     4441 tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc aatggtgccg
     4501 ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga aaacagactc
     4561 agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc catatagtgt
     4621 gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa ctgctggata
     4681 acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag ataggcgcgc
     4741 acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac cagcaccacc
     4801 gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat cgccaccagc
     4861 atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac gtagagatcg
     4921 cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg ttgggtatca
     4981 aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa ctgttgcact
     5041 gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc ggcggcaaac
     5101 agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg caggttattt
     5161 ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac ttctgccgcc
     5221 gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg cgaaccatac
     5281 gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga cggcctcctc
     5341 gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat acatatcgcg
     5401 ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga tatgtccgtg
     5461 ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg cactgatgat
     5521 taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct ctgtgcgtcc
     5581 gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta ataacgctcg
     5641 agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct cgcccacttc
     5701 tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta accgcgcgac
     5761 atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat tcgccacggt
     5821 gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc agctatcgag
     5881 ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga cgtcgaaatg
     5941 acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc aaatacccag
     6001 catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt cagtctgcgg
     6061 atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca cgggttcgct
     6121 accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt atgcagcact
     6181 accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa tcatcagacc
     6241 taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca ccaccatcca
     6301 actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga tggtcgggtc
     6361 gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt cggcatccgc
     6421 ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc ggatactggt
     6481 gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca gcttaaagcg
     6541 ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca tcggtaacag
     6601 ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca cagcgcagcc
     6661 cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca cgacgcgatc
     6721 gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt aaaactccgg
     6781 gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca gttggtaaga
     6841 cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa cggcaatcag
     6901 caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt ctgtcacgcc
     6961 atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga taatgacaag
     7021 caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc ttaattgagc
     7081 aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat gggtaaagaa
     7141 gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag ccccggcacg
     7201 ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac ctggacgcgc
     7261 atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg catttcggta
     7321 accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt tatcagcaca
     7381 aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt gccgataagg
     7441 ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt cagattaacg
     7501 gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc cagcagctta
     7561 cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt taattctatt
     7621 ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc acaaaatgac
     7681 aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc tcgttattcg
     7741 ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga gacagcctga
     7801 tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg cggcatcgca
     7861 ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt catcgcatca
     7921 ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac cgtcaatgca
     7981 gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga aaaacacaaa
     8041 tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag atcgtcgtga
     8101 tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac atcgattatc
     8161 tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc tttcccggta
     8221 aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg ctgatacagc
     8281 accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc ttccagagaa
     8341 tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc cccgagaata
     8401 tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc gcgttcacgc
     8461 agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat caataaagtg
     8521 ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg acacggttaa
     8581 tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa ataatattgt
     8641 tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg tggcaacctt
     8701 cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag cgaacgccat
     8761 gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca ggcgcagtca
     8821 ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct acgcaatgcg
     8881 atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct ggcgcaactg
     8941 gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg gcaaaacgat
     9001 acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc aggctttttg
     9061 cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac ccgcttcgat
     9121 aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc tactgacagg
     9181 ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga tgcagtaaaa
     9241 gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact ggatcaatta
     9301 tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt ctttttacat
     9361 cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct ggcgcatccg
     9421 caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc ggagctggtg
     9481 cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc ggttgagtac
     9541 tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca tcccgtgcaa
     9601 atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag tggcatcagt
     9661 ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg gcaggcgcga
     9721 aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt cggagaaaac
     9781 ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg gcaagtaccg
     9841 gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat agaacttcat
     9901 accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact cacaattatt
     9961 tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga cctcatgatg
    10021 ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct acgttgcgat
    10081 ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc gacgctggac
    10141 gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc ggtttttagc
    10201 cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg cattagtgcc
    10261 cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt taccgaagcc
    10321 tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc gttaccataa
    10381 cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt tcagtggagg
    10441 ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag tccattgcgt
    10501 gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg aactggcgct
    10561 gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg agctggcggt
    10621 cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag ccgatggcat
    10681 cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg caatatatgc
    10741 gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt tctccggcta
    10801 cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt tgggcatggc
    10861 gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa cctgggcgct
    10921 ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct gctataacgg
    10981 cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca acggtttgaa
    11041 tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa ccgggttgag
    11101 gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa aacgtttact
    11161 taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct ttctgcgttt
    11221 taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct tccggctcca
    11281 gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac tgactggtaa
    11341 tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata ttgaccacga
    11401 cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc accccatccc
    11461 agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg taaacgtcga
    11521 gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac gttgttgtta
    11581 taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt gccactatgc
    11641 aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg cccatccccg
    11701 gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga taactgacaa
    11761 tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg
    11821 cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt actgctgctt
    11881 tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc gccacagaag
    11941 ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta atgagcagtg
    12001 ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa tcaccttaac
    12061 gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac
    12121 cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat tattgctgtt
    12181 ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc agagcttcaa
    12241 agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc tgatcttccg
    12301 gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct tgcatatcgg
    12361 tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc
    12421 taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc agaccggcag
    12481 cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc tcagcatcca
    12541 ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt tcctgaacat
    12601 ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg aatgcgcggt
    12661 cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca cctaccagac
    12721 cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc aggttatctt
    12781 tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca ccggccagag
    12841 actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag tacgcatcga
    12901 gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct aacgatttca
    12961 ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa gatttgatga
    13021 caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg gggatagtgg
    13081 catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg gcagattttt
    13141 ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc gctttcgctt
    13201 catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg gtgaattgat
    13261 cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc tgcatcgttg
    13321 cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt
    13381 tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc gccagcgcgt
    13441 taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc gccagctcgt
    13501 cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc tgttgggtag
    13561 tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc tggttgagga
    13621 taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa
    13681 tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg tactgatcca
    13741 acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc atatagcctt
    13801 cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga ttacgctcgc
    13861 tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact tttgcggcgt
    13921 agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac gacacgataa
    13981 tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca
    14041 tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc
    14101 aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac agaaagtcac
    14161 cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc acatccccac
    14221 tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga agctcggtac
    14281 tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag tacgggcagc
    14341 acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc cttctttcag
    14401 agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag ctttagagat
    14461 atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc tcttcctctt
    14521 tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc
    14581 agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc taacttagcg
    14641 agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta ttagtgcatt
    14701 ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca ttttgcagcg
    14761 ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg gcaattacgt
    14821 tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact gttttaatcc
    14881 cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt acctgaccac
    14941 gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg gtcaggcaag
    15001 aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc gtcgcacctt
    15061 ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc cccagttttt
    15121 ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc acttcgccga
    15181 gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca atggtcagca
    15241 agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg ttttcgttat
    15301 cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta tcgccgttaa
    15361 tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg cggcacagtt
    15421 tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa cgaataatgc
    15481 cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta cgttcaatct
    15541 gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta taaccggaga
    15601 ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag ttcgacgtcg
    15661 cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg ctgaacgcta
    15721 cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct cgcatgtcag
    15781 aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc actttcgcca
    15841 ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga ccacggattt
    15901 ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg gtggctttgg
    15961 caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg cagaggatcg
    16021 gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg cgatctttca
    16081 cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt
    16141 tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa ccacgcacta
    16201 cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct gcttccgctt
    16261 tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg gcttcgtttt
    16321 cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt tgctcgttca
    16381 gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga atgcgtttct
    16441 caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc agacgttcgt
    16501 taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc atatgtgcag
    16561 caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc agcacttctg
    16621 gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca gtacgcacca
    16681 gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc ttcgcagaaa
    16741 agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca
    16801 ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg gtcccgacgg
    16861 tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg accagcataa
    16921 tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc cgcccgacaa
    16981 ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat acggggattt
    17041 caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt tccgccaggt
    17101 aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag tatatgggga
    17161 tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga taaaatcccc
    17221 cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc ctgttgcgct
    17281 tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga ctcgtcgata
    17341 accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag cagtgcggct
    17401 tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt cttagcgata
    17461 gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa cagcgcctga
    17521 tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc ttcttcgctc
    17581 agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat aagaccaaac
    17641 ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac
    17701 ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg gtgggaaatc
    17761 actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga ggtatcaacc
    17821 tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc agctaccgta
    17881 ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc tcgggtaatg
    17941 gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac aatcccgcgc
    18001 tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat gttttcaacg
    18061 tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt gaacgggacg
    18121 tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt cggaccgatc
    18181 agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt gcgcagacgt
    18241 ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg gccgataacg
    18301 taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc
    18361 ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg
    18421 cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct tttgccgcaa
    18481 aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac
    18541 ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag gcgcaagccg
    18601 cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc tggcatcaat
    18661 tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg gcggaaagga
    18721 agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta tgaagcgcca
    18781 taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt tcgatatcgg
    18841 tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa ttcggcaggc
    18901 aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag gccgcctggc
    18961 ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag atagacatcc
    19021 cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt tctgggtttt
    19081 ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct tcaacctggc
    19141 cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa gagcgctcac
    19201 cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca aagttatctc
    19261 gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc tgctgtaccg
    19321 attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg gcatcaccat
    19381 atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt ttcgcacggc
    19441 tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg cctttgtgcg
    19501 aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg ttgaaagtgg
    19561 tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt tcttccagag
    19621 caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg ataacttctt
    19681 tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc tcttcgtcag
    19741 ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact acgcggcgtt
    19801 tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg ttgccaccga
    19861 aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc gcagccggta
    19921 cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg cggttacgga
    19981 tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga ccttctacgg
    20041 aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc agttcacgct
    20101 cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg ttttctgcgt
    20161 ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg tggcctttga
    20221 taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg aaatcagacg
    20281 ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg gttacgcggt
    20341 cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta cgcagagtat
    20401 ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca acttcgatcg
    20461 cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag taagtgaagt
    20521 cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga ttgatttttt
    20581 ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg tcctggcgta
    20641 cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg cggaagccgt
    20701 caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc tcgatgctgt
    20761 cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca actgaaactt
    20821 gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc aagcacaggt
    20881 tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt taccagaatc
    20941 atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta tgagtcgaga
    21001 acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt cgtctgaatg
    21061 ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt ttttcataaa
    21121 atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta cgcgatgctt
    21181 cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc ccactcctta
    21241 atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc cgctaaagta
    21301 ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc gctgaccagc
    21361 acaactttaa aatggctttc agagccggct gggacattgt gacgatagct ttcatccact
    21421 acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg ctcacgtatc
    21481 atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa atactagccg
    21541 cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct ggtttttccg
    21601 tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat cagcgtttct
    21661 tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc cacttcccct
    21721 cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag agtctctgag
    21781 agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat gcttgcagga
    21841 tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc acagcaggat
    21901 ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac cgatcaggcg
    21961 ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg cgctctgcgt
    22021 ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat ggttggtccg
    22081 aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt gtcccagggc
    22141 aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac cgcgcagaat
    22201 ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc gttccaggcc
    22261 tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat cgctgatatg
    22321 tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt tctgccctct
    22381 ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac gtatttttca
    22441 acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac cgctcgccct
    22501 gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca aaaccattgg
    22561 tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac cgctgatgga
    22621 ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca cgcaaatcct
    22681 gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac tccgctggat
    22741 ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg tcttcgatgc
    22801 gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa tcagcgtgct
    22861 gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg cagataaatg
    22921 gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct gtattatcgc
    22981 gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc tggaacaagc
    23041 ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta ttttgcttct
    23101 tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca cgtttttgat
    23161 tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc ttggcttact
    23221 ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt cgctgttccg
    23281 ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact ggctgctgtc
    23341 gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa acctctgtgg
    23401 cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata agtcattttc
    23461 cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt atgtcggccc
    23521 cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat tctccgtcgc
    23581 cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg aatatacacg
    23641 agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct ttgccatgtg
    23701 gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga cgatggacgc
    23761 gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg ttttagtcgc
    23821 cctttctggc gtcgtgcttg gtggtttgct g
//
LOCUS       DAJVQR010000059        23760 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVQR010000059 DAJVQR010000000
VERSION     DAJVQR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..854)
                     /gene="rluF"
                     /locus_tag="OQM52_002491"
     CDS             complement(<1..854)
                     /gene="rluF"
                     /locus_tag="OQM52_002491"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1564303.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGR"
     gene            1202..2149
                     /locus_tag="OQM52_002492"
     CDS             1202..2149
                     /locus_tag="OQM52_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCS1564304.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2219..3025
                     /locus_tag="OQM52_002493"
     CDS             2219..3025
                     /locus_tag="OQM52_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1564305.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3035..3442
                     /locus_tag="OQM52_002494"
     CDS             3035..3442
                     /locus_tag="OQM52_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS1564306.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3442..3936
                     /locus_tag="OQM52_002495"
     CDS             3442..3936
                     /locus_tag="OQM52_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCS1564307.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4002..4799
                     /locus_tag="OQM52_002496"
     CDS             4002..4799
                     /locus_tag="OQM52_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1564308.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4810..5598
                     /locus_tag="OQM52_002497"
     CDS             4810..5598
                     /locus_tag="OQM52_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1564309.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5650..6879
                     /gene="sorE"
                     /locus_tag="OQM52_002498"
     CDS             5650..6879
                     /gene="sorE"
                     /locus_tag="OQM52_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1564310.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7261..7950
                     /gene="pepE"
                     /locus_tag="OQM52_002499"
     CDS             7261..7950
                     /gene="pepE"
                     /locus_tag="OQM52_002499"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1564311.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8041..9672)
                     /locus_tag="OQM52_002500"
     CDS             complement(8041..9672)
                     /locus_tag="OQM52_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1564312.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9892..13575)
                     /gene="metH"
                     /locus_tag="OQM52_002501"
     CDS             complement(9892..13575)
                     /gene="metH"
                     /locus_tag="OQM52_002501"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1564313.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13775..14599
                     /gene="iclR"
                     /locus_tag="OQM52_002502"
     CDS             13775..14599
                     /gene="iclR"
                     /locus_tag="OQM52_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1564314.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTYRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            14916..17100
                     /gene="espL4"
                     /locus_tag="OQM52_002503"
                     /pseudo
     CDS             14916..17100
                     /gene="espL4"
                     /locus_tag="OQM52_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17069..18806)
                     /gene="aceK"
                     /locus_tag="OQM52_002504"
                     /pseudo
     CDS             complement(17069..18806)
                     /gene="aceK"
                     /locus_tag="OQM52_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(18990..20294)
                     /gene="aceA"
                     /locus_tag="OQM52_002505"
     CDS             complement(18990..20294)
                     /gene="aceA"
                     /locus_tag="OQM52_002505"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCS1564315.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20324..21925)
                     /gene="aceB"
                     /locus_tag="OQM52_002506"
                     /pseudo
     CDS             complement(20324..21925)
                     /gene="aceB"
                     /locus_tag="OQM52_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22194..23123)
                     /gene="metA"
                     /locus_tag="OQM52_002507"
     CDS             complement(22194..23123)
                     /gene="metA"
                     /locus_tag="OQM52_002507"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1564316.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23280..23723
                     /locus_tag="OQM52_002508"
     CDS             23280..23723
                     /locus_tag="OQM52_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1564317.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5852 a         6059 c         6032 g         5817 t
ORIGIN      
        1 cgccccggcg aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt
       61 ttttccatct taacgactgg acgtttgatg cccgctgttt tcggtttggc cttcgcttta
      121 ggttttgcct cggaagagga attttcgatg agcttaaaga ggtcgattaa ctcatcgtcg
      181 gttaaatcgc gccactcccc cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta
      241 cgttccagct ttttcacttc atagccgaaa tgctcgcaca tgcgccggat ctgacggttc
      301 agcccctgca ctagggtaat gcggaagaca aacggcgctt cttttttgac tttgcacttt
      361 ttcgtcaccg tcccgaggat cggcaccccc gcccccatac cacgaataaa ttcatcggta
      421 atcggtttat cgaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc
      481 ttattcacca gatcgccgtg attggtgagg aaaatcagcc cctgggagtc tttatccagg
      541 cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg
      601 ccatcttcgg tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct
      661 tcggcttccc gaggctcaat caactgaccg tttactttca caatgtcgcc gggtttcacc
      721 tgatcgccaa tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga
      781 tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat
      841 gagtcgggca gcataaattc tcctgtaaaa cgcggaatat accgtacctc ttagcgggga
      901 aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa
      961 aagtgtctcc ggaagtacga tgcaaatttg cctggcaaaa taaggataat caacatatta
     1021 cgaatcgctg cacacctgtg ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa
     1081 gcgctatttc tccccatgca aagagtgaaa aatcgctttg aaaggccctc atgatggggt
     1141 tatatcagat caagcggtga gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc
     1201 aatggaaaac agtgacgata tccgtttgat tgtgaagatt gcccaactct attacgaaca
     1261 ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt taccgcacca ccatcagccg
     1321 cttgcttaaa cgaggccgcg atcagggaat ggtcaccatc gccatcaact atgactacaa
     1381 cgaaaatctc tggctggagc agcaactgaa gcaaaagttt ggcctgaaag acgttgtggt
     1441 ggtgtcggga aatgatgagg atgaagagac tcaactggcg atgatggggt tacacggcgc
     1501 gcaactgctg gatcgcttgc tggagcctgg cgatattgtc ggtttttcct ggggtcgcgc
     1561 ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt taatctgcgt
     1621 accaattatt ggcggaccgt ccggtaaact cgaaagccgc tatcatgtaa acacattaac
     1681 ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc gcggattttc cggctctgct
     1741 ggataaccca ttaattcgta atgggatcat gcagtctcag cactttaaaa ccatctctgc
     1801 ctactgggat aatctggata tcgccctggt gggaattggc tcaccggcca ttcgcgacgg
     1861 cgctaactgg catgcgtttt atggtggtga agagagtgac gacctgaatg cccgccaggt
     1921 tgctggcgat atttgctcgc gcttttttga tattcacggc gcaatggttg aaacgaatat
     1981 gagcgaaaaa acgctctcta tcgaaatgaa taaattaaag caggcgcgat attccattgg
     2041 cattgccatg agtgaagaaa aatacagcgg aattattggt gcactgcgtg gaaaatatat
     2101 taattgtctg gtaacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg
     2161 atgtttaccg taataaaaat tacggcgggt ccctattatc taaaatcagg agtctgttat
     2221 gcaaacgtgg ttaaatttgc aggataaaat cattattgtc accggcggcg catccggtat
     2281 tggtctggcg attgtagagg aattattagc acaaggcgcg aatgtacaga tggtcgatat
     2341 tcacggtggc gatggtcaat atgaaggtca taaaggctat cagttttggc cgaccgatat
     2401 ttctagcaca aaagaggtaa atcatacggt agcggaaatt atccagcgtt ttggtcgcat
     2461 cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc
     2521 gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca
     2581 gaaaggtgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg
     2641 cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc tcagaaggac aaagctgtta
     2701 cgccgctacc aaagccgcgc tcaatagctt cacgcgctcc tggtcgaaag agctgggtaa
     2761 acacggtatc cgtgtggtcg gtatcgcgcc cgggattctg gaaaaaaccg ggctacgcac
     2821 gccggaatat gaagaggcgc tggcgtggac gcgcaatatc accgtcgagc agctgcgtga
     2881 aggctatacc aaaaacgcca ttcctattgg gcgcgccgga agattagcag aagtagctga
     2941 ttttgtttgt tatctgttgt ctgaacgcgc cagctacatc accggagtaa ccactaacat
     3001 tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc aacgccattt tttgtgccca
     3061 cggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtttacggca acgccaatgt
     3121 tgaagccgtt gcatttgtgc ccggtgaaaa cgccggagac attgcggtaa agctggaaaa
     3181 gttagtaagc attcacaatc aagatgagtg gctgattgcc gtggatttac agtgcggcag
     3241 cccgtggaat gcggcagcaa tgcttgcgat gcgcaatcct cgtttgcggg taatcagcgg
     3301 cctttctctg ccgctggcgc tcgaactggt tgataaccag gacagcatga atgttgatga
     3361 actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc
     3421 aacgacagag gaggatttct gatgaacatt acgctcgccc gcattgatga ccgtctgatc
     3481 cacggccagg tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat tattatctgc
     3541 aatgacgaag tttataacga tgaagtgcgc cggacattat tgcgtcaggc tgcgccaccg
     3601 ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg cggtttatca caacccgcaa
     3661 tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcgatggtt
     3721 cgccaggggg taaaaatagg caccctgaat attggcggca tggcctggcg gcccggtaaa
     3781 aaacagttaa ccaaagcggt ttcattagat gatgatgata ttaacgcatt tcatgagtta
     3841 aataatcttg gtgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt
     3901 atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcctgt gtacatatgc
     3961 tttaacaggc agggatgacg acacttaaaa ggtgacaaat catggaaata agtaccctac
     4021 aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat
     4081 ttcagactca tcgcccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa
     4141 aaaccggaat aatactcggc ggtacgctgg aattgatagc tctctgctgg atgaacgtcg
     4201 gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg
     4261 ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc
     4321 aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag
     4381 cagcagaaga ggcgcggttt cgcactctcg atattctgca tgtctccgcg cttggcgtgc
     4441 aggcgctgcg cgtcgccatt ccggcactga ttgtctcact gttcgtcagc gccgatatgg
     4501 tcagcaatat gttaaacgcc attcccgaat ttgtgacccg aggactacag attgctggcg
     4561 gttttatcgt ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg aaatatttga
     4621 tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagc ctgctggcgt
     4681 tcggtggcgt cggcgtgatc atggccctgg tctacatcca gctaaatcca cagtggtgta
     4741 aagctgaacc tcaactccag gccaccgcct ccaccgccct tgaccaactt gacgattaac
     4801 ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc
     4861 tccaacctgc aacaggcgtc ctttaacttt gaacgtattc acgggctggg cttttgctac
     4921 gacatgatcc ccgccatcaa gcgactttac ccattaaaag aggatcaggt tgcggcgctc
     4981 aggctacacc tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgcg
     5041 gaaattgatg acggtaccat caacggcatc aaagtcggtc tgatgggacc attggcagga
     5101 gttggcgatc cactggtctg gggaacgctg cgcccgatta ccgccgcgct cggcgcatct
     5161 ctggcacttt cgggcaacat tctcggcccg ctgctgttct tctttatttt caacgcggtg
     5221 cgtctggcga tgaagtggta tggcctacag ctcggctttc gcaaaggggt gaatatcgtc
     5281 agcgatatgg gcgggaatgt gctgcaaaaa ctcaccgaag gcgcgtcgat tctcggactg
     5341 tttgtgatgg gcgtgctggt gaccaaatgg acgtcaatca acgtaccgtt ggtggtttca
     5401 caaacgcatg ccgccgatgg ctccaccgtc accatgaccg tgcagaacat tctcgaccaa
     5461 ctttgccctg gtttgctggc gctcggtctg acgctactaa tggttcgtct gctcaacaaa
     5521 aaaattaacc cggtatggct gattttcgcc ctgtttggct tagggattat cggcaatgct
     5581 ctgggcttcc tgtcctgatt cttcgccccg gcacgactgc cgaggccatc gctcaacatg
     5641 aggtggttta tgaaaacaac agctctgcgt ctttatggta aacgtgattt acgcctggaa
     5701 acctttgacc ttcctgaaat gcaggaggat gaaatcctcg caacggtggt cactgacagc
     5761 ctgtgcctct cttcctggaa agaggccaat ctgggtgaaa accataaaaa agtacccgac
     5821 gatgtgacga ccaacccaat catcatcggc cacgaatttt gcggcgatat tctggccgtg
     5881 ggtaaaaagt ggcagcacaa attccagccg ggtcagcgtt atgtgattca ggccaacctg
     5941 caactccccg accgcccgga ttgccccggc tactccttcc cgtgggtaga cggcgaagcg
     6001 acgcatgtgg ttattcccaa cgaggtcatg gaacaatatt gcctgctggc atacgacggc
     6061 gaaacctatt ttgaaggctc actggttgaa ccgctttcct gcgtgattgg cgcgttcaac
     6121 gccaactatc atcttcagga aggtagttat aaccacacga tggggattcg cccgcaaggg
     6181 cacacgctga tcctcggcgg caccggacca atgggactgt tggcgattga ttatgcgctg
     6241 catggaccag ttaacccgtc gctgctcgtc attaccgata ccgacaacga caaattgagt
     6301 tatgcgcgca agcactatcc atcagaaccg caaacactga ttcattatct caatgccacc
     6361 gatgcagcat ttgatacgtt aatggcgctg agtggcggtc acggcttcga tgatattttc
     6421 gtctttgtgc ctaatgaagg actggtgact ctcgcctctt ccttgctggc gacagatggt
     6481 tgcctgaatt tcttcgccgg accgcaggat aaacatttca gcgcgccaat taatttctac
     6541 gatgtgcatt atgcatttac ccactacgtg ggcacgtcag gcggcaatac cgacgacatg
     6601 cgcgcggcgg tcaaactgat cgaagagaaa aaagtgcagg ccgcaaaagt ggtaacacat
     6661 attcttgggc tgaatgccgc gggcgaaacc acgcttgaat tgcctgccgt cggcggcggc
     6721 aaaaagctgg tgtataccgg gaaatacctg ccgctgacgt cactcacgca gattcaggat
     6781 caagcactgg cggcgattct ggcgcgtcat caggggatct ggtccggtga ggtggagcaa
     6841 tacctgctcg cccatgcaga ggcaatttcc catgattaat cgcgctactt aactctgcat
     6901 gtcgctggct ggtcgcccca gcaacttcgc gaccggatgt gcggctgatt gctgaagcag
     6961 cacaatttgc acgagaagga taaaaaatat cagcgcctta aggggcgctc aaattggtgg
     7021 caaagtacac attgtttatg ccggatgcgg aatgaagcct tattcggtct acataatttt
     7081 gaaaatccaa tatattgcag ttatctggta ggccaggtaa gcgtagcgca tcaggcaatg
     7141 ctgtgtttgt catcagtctc agcgcccggt gcaatacgtg acatcgtgag ccatgtcagt
     7201 aagtggttaa attcgcagtg gtagcatgcc ttccgtgatt tcttactaac cggagaagta
     7261 atggaactgc ttttattgag taactcgacg ctgccgggta aagcctggct ggaacatgca
     7321 ctgccgctaa ttgctgaaca gttgcagggt cgccgctcag cggtgtttat ccctttcgct
     7381 ggcgtaacgc agacctggga tgattacaca gagaaaacgg ctgcggttct cgctccgctg
     7441 ggtgtttctg tcaccggtat tcatagcgtt gtcgatcccg ttgccgcgat tgaaaatgcg
     7501 gaaattgtga ttgtcggcgg cggtaatact ttccagttgc tgaaacagtg ccgcgagcgc
     7561 gggctgctgg ctccaattac tgacgtggtt aaacgtggcg cgctgtatat tggctggagc
     7621 gcaggcgcta accttgcttg cccaaccatt cgtaccacca acgatatgcc gattgtcgat
     7681 ccgcaaggtt tcgatgcgct aaatctgttc ccgttgcaaa tcaacccgca cttcaccaac
     7741 gcgctgccgg aaggccataa aggtgaaacc cgtgagcagc gtattcgcga actgctggtc
     7801 gtcgcgccag aactgacgat tattggtctg ccggaaggta actggatcac agtgagtaaa
     7861 ggtcacgcta cgctgggtgg cccgaacacc acttatgtgt ttaaggctgg tgaagaagcg
     7921 gttccgctgg aagctggtca ccgtttttaa gtgaattgcc ggatgcatga tccggcatct
     7981 tgttcatagt gcctgatgcg acgcttgacg cgtcttatca ggcctacagg attcacaata
     8041 ttaatactcg tcccgtcctt catcttcatc cggctgttcc agcacactgt aagccaccga
     8101 acaaaacagc gagttcaggc gctgcatatc tcccagtaag cctaaatgta gcgaactggt
     8161 ttcaatgctc tgcacgtttt gctgatgcag gcgatcgacg tgggcgtggg aatagcggcg
     8221 attaagaatg cgaaaacgat gtttgctgcg acgcaaacga cgagcgctgg tgacatcgcc
     8281 agagaagaac accgacattg ccagctttaa attactgagc aattgctcat agagcgcatc
     8341 cagttccttc aacccatcaa gcgaaaatgc ccgccgtgct gccagcgatt tatcggcaat
     8401 ttcgctgccc atgcgctcga cgatatcgga ggcctgttca aggttgagcg acatttcgat
     8461 gatctccgcc cagcggcgcg actcttcctc cgccaactct tcttttggca tccgcgccag
     8521 atacagctta atggcggtgt agagaacgtt gatatcatct gccagcttac gcagctcttt
     8581 ctcctgccgt ggctcgccgt gcatcacttt attcagccct tccatcatct gttccatggc
     8641 gtcgccaatg cgcagggttt cgcgcgcggc gttcgccaga gcaagcgtgg gcgtgtccag
     8701 cgcgctgaca tccagatgtt taggccgtag ctgggtatcc agttccggtt catcgcgaat
     8761 aatcgttttg caaaaccgtg ccatcgggtc aacaaatggc agcatgacca ggcaacgtac
     8821 aaggttgtag aagacgtgga aatagatcac cagttccgct tttggcaatg acagcttccc
     8881 cattgtctct gccagcaaat ggacaaacgg caggataatc aggctaccca ccagcttaaa
     8941 cagcagacta cccagcgcga cacggcgggc tgcggcattg gcggcactgt tgttgagcat
     9001 cgccagcagg ccggaaccga ggttagctcc aatcaccaga cagagcgcca cggggaagga
     9061 gataatgcct gcagcggtca gagtcgcggt cagcagtaca gcagcaaggc tggagtagct
     9121 gataatggcg aacatcgcgc caatcagcgc atccagcaga atatcgccgg tcagcgaggc
     9181 aaagatcacc tgaacgccgt ttgcctgggt gatcggcgtt acggcctgca caatcaactc
     9241 cagcgccagc aaaatcagcc caagaccaat accgacgcgg cccagttgcc cggcgcgtga
     9301 ctgtttgcgt ccgaggaaaa aaatcacgcc gataaaaata agtaacggtg acagccagga
     9361 taagtcgaag gtgagaatac gcgccattag cgccgtcccg acatctgccc ccagcacaat
     9421 gaccagagcc ggtgcgaggg ctaccagatc ctgagcgacg aacgaggtca ccagcatggt
     9481 ggtggcatta ctgctctgta ccagtgcggt aacgccgatc cccgcgcaaa aggcgagcgg
     9541 ctttttttcg acgctccggc taaggacagt acgcaaacgc gcgccgaaga cgcgcattac
     9601 gccggttcga acaatatgag tcccccagac cagcagggcg acggcagaaa gcaggtgaag
     9661 cagcgttaac acgagttcag attctcctta tcgttatatg ttcctgaagc aattgaatga
     9721 ccaaaggacc ggaaacacgc tgactggcat ctttactgtg ccttttgaca ttcgtttttc
     9781 cggctaagat tattccagcc tgtttccagt ataagtgttt catattaata aagagacagg
     9841 gcactcattg agcgccctgt ttgttgtaag gaaaagtgac agatttgtga atcagtccgc
     9901 gtcatacccc agattcggtg ccagccagcg ctcaacatcg gagacgctca tacctttacg
     9961 gcgggcatgg tcttcaacct gatcgcgctg aatttgcgcc accgcgtagt acttgctgtc
    10021 cggatgactg aaataccaac ccgaaaccga tgcaccaggc cacatcgcga aagattctgt
    10081 gagtttcatg ccagtatgtt tttccacttc cagcagctcc cagatggtgg ctttttccgt
    10141 atgttccggg caggccggat agcccggtgc cggacggatg ccctggtagt tttcgcggat
    10201 cagttcttcg ttgctgaggt tctcgttcgg cgcatagccc cagtagactt tacgcacacg
    10261 ctcatggaga tactccgcaa aggcttcggc taaacggtcg gcaagcgctt tcaccatgat
    10321 tttgttgtaa tcatcgtgct gcgcttcaaa ggcatcagcc agtgcgtcct cttccagccc
    10381 gccagtcacg gcaaatgcgc cgatgtaatc tgctttacca gaaagcttcg gcgcaacgaa
    10441 gtcagcgaga cagtagttag cgaatcccgt tttttcggtc tgttgacgca gatggtggct
    10501 gacgttgatc acatgggtgc gcgtttcgtc acggtagatt tcaatgtcat cgcccacacg
    10561 gtttgccggg aacaggccca ccacgccacg cggattcagc gttttctcgg cgcttaattt
    10621 atccagcatg tcgttggcgt ctttaaacag ccgctgcgcc tcaacgccca ccacttcatc
    10681 ttccagaatg cgaggatact tcccggccag cgaccaggtc ataaagaacg gtgtccagtc
    10741 gatgtaatta cgcagcgttt cgatgctggc ttccacttcc tgcacgccga gacggtgcgc
    10801 caccggcggc gtgtaagcct gccagtcaaa tgcgaaatcg ttatcgcgcg ccgcttccag
    10861 cgtgaccggt ggtgtgcgtg gtttcttgcg cccgtgctga atacgcacgg tttcgtactc
    10921 cttgcaggta cgagcgacaa aatcatcacg ctgagtatcg gaaagcagcg ccgctaccac
    10981 accaacggtg cgcgaggcat tctgcacata caccgtaggg ccgctgtagt tctgctcgat
    11041 tttcaccgcc gtgtgcgctt ttgaggtggt cgcgccgcca atcagtaacg gaatggcgaa
    11101 gccctgacgc tccatctctt tcgccacgtt aaccatctcg tccagcgacg gcgtgataag
    11161 ccccgaaagg ccaatcaaat cagcattcac ttctttggcg atacggagaa ttttttccgc
    11221 aggcaccata acgccgagat cgacaatttc gtagttgtta cattgcagca ccacaccaac
    11281 gatattttta ccgatgtcgt ggacatcgcc tttcacggtg gcgatcacca tcttgccgtt
    11341 ggttttaccc tgctctttgc tggcttcaat aaacggttcg agatacgcca ctgcctgttt
    11401 catgacgcgc gccgatttga ccacctgtgg caggaacatt ttgccttcgc caaacagatc
    11461 gccgaccacg ttcatgccgt ccatcaacgg gccttcaatc acttcaatcg ggcgcgtagc
    11521 ctgctggcgg gcttcttcag tatcctgctc gataaactcg gttatgcctt tgaccagcga
    11581 gtattccaga cgtttattca cgtcccacga gcgccactcc gcttgctggg cgttagcagt
    11641 gtcgtccgct ttactgccgc gatatttctc ggcaagctcc agtaaacgct cagtgccatc
    11701 gtcacgacga ttaagaatca catcttccac cgcgtcgcgc agttcagcgg gtaggtcgtc
    11761 gtaaatcgcc agttgcccgg cgttgacgat ccccatatcc atgccattgc gaatagcgta
    11821 gtagaggaac actgcgtgaa tggcttcgcg caccggatcg ttgccacgga atgagaaaga
    11881 aacgttagat acgccgccgg aaatcagcgc gtgcggcagt tcgcgtttga tgtcttcaca
    11941 cgcgccgata aagtcctgcg cgtagttgtt gtgctcttca ataccggtgg cgaccgcgaa
    12001 gatgttcggg tcgaagatga tatcttctgg cgggaagcca acctcttcgg tgaggatttt
    12061 gtacgcccga cggcaaatct cgattttccg tgcgcgagta tcggcctgtc cctgttcgtc
    12121 aaaggccatc accaccactg ccgcaccgta gcgacgcaac aatttcgcgt gatggataaa
    12181 ggcgtcgacg ccctctttca tcgagataga gttaacaatg cctttgccct ggatacactt
    12241 cagacctttt tcaatgacat cccattttga ggagtcgatc ataatcggca cgcgagcgat
    12301 atccggttca ccggcaatca gattgagaaa acgcaccatc gccgcttcgg catcgagcat
    12361 cccttcatcc atgttgatat cgataatctg cgcgccgttt tccacctgct gacgcgccac
    12421 atccagcgcc tcgctgtatt tctcttcttt gatcaggcgc ttgaacttag cagagccggt
    12481 gacgttggtg cgttcaccca cgttcacaaa caggctatcg tcgccaatgt tcagcggctc
    12541 caggccagac aaacggcagg ctacgggaat ttccggcagt ttgcgcggcg ctaatccttc
    12601 tactgcacga ctcatcgctg caatatgttg cggcgtggtc ccgcagcaac caccgacgat
    12661 attgagaaag cccgcttgcg cccattcacg tatctgtttt gccatcgtgt cggcgtcgag
    12721 atcgtattca ccaaaggcgt taggcagccc ggcgttcggg tgggcggtga cgtagcattc
    12781 cgcaatccgt gatagttcct gcacgtactg gcgcagttca tcgggcccca gcgcacagtt
    12841 caggccaaag gtcagcgctt cggcgtggcg caatgagttg taaaatgctt cggtggtctg
    12901 cccggagagc gtgcgcccgg aggcgtcggt gatggtgccg gagatcataa tcggcagctc
    12961 aacgcccagc gcttcaaact ccgttttcac cgcaaagacc gccgctttgg cgttaagggt
    13021 gtcgaaaacg gtttcaatca ggatcagatc cgcaccacct tccaccagcg ctttggtgga
    13081 ttcgcgatag gccgccgcca gctggtcaaa agtgatgttg cggaatgccg gatcgttgac
    13141 gtccggagaa atagacgccg tgcggttggt tgggccaaga acaccggcaa cgtagcgcgg
    13201 tttctccggc gtgcgcgcgg tccactcgtc agcacaggcg cgcgccagtt tcgccgccgc
    13261 aaagttgatt tccgccgaca gggattccat ctggtaatcc gccatcgcaa tggtcgtgga
    13321 gttgaaggtg ttggtttcga tgatatccgc gcccgcttca aagtaggcgt tgtggatagc
    13381 ggcgatcact tccggtttac tgagtaccag caggtcgttg ttgcctttga ggtcgcatgg
    13441 ccagtcggca aagcgttcac cacgaaaatc ggcttcgttc agtcgatagc tctggatcat
    13501 ggtgcccata ccgccgtcca acaccagaat acgttcattt aactgcgcac gcagttgttc
    13561 cactttgctg ctcacacttg ctcccgacac gcttaaccca gatttaataa aaattcaaca
    13621 aaccatactg gaataaacgc atctgtggta aaagcgacca ccacgcaaca tgagatttgt
    13681 tcaacattaa ctcatcggat cagttcagca actattgcat tagctaacaa taaaaatgaa
    13741 aataatttcc acgatacaga aaaaggaggc tgtcatggtc gcacccattc ccgcgaaacg
    13801 cggcagaaaa cccgccgttg ccaccgcacc agcgactgga caggttcagt ctttaacgcg
    13861 tggcctgaaa ttactggagt ggattgccga atccaatggc agcgtggcac tcacagagct
    13921 ggcgcaacaa gccgggttac ccaattccac gacctaccgc ctgctaacca cgatgcaaca
    13981 gcagggtttc gtgcgtcagg tcggcgaact gggacactgg gcaatcggcg cacatgcctt
    14041 tatggtcggc agcagctttc tccagagccg taatttgtta gcaattgttc accctatcct
    14101 gcgcaatcta atggaagagt ctggcgaaac ggtcaatatg gcggtgcttg atcaaagcga
    14161 tcacgaagcg attattatcg accaggtaca gtgtacccac ctgatgcgaa tgtccgcgcc
    14221 tatcggcggt aaattgccga tgcacgcttc cggtgcgggt aaagcctttt tagcccaact
    14281 gagcgaagaa caggtgacga agctgctgca ccgcaaaggg ttacatgcct atacccacgc
    14341 aactctggtg tctcctgtgc atttaaaaga agatctcgcc caaacgcgca aacggggtta
    14401 ttcatttgac gatgaggaac atgcgctggg gctacgttgc cttgcagcgt gtattttcga
    14461 tgagcaccgc gaaccgtttg ccgcaatttc tatttccgga ccgatttcac gtattaccga
    14521 tgatcgcgtg accgagtttg gcgcgatggt gattaaagcg gcgaaggaag tgacgctggc
    14581 gtacggtgga atgcgctgac tttttctggc gggcagaggc aatattctgc ccatcatacc
    14641 tgagtggcaa tagaataagg gtgtctgtta atcgcattga cgccaaaata acttaatgtc
    14701 atacacttca cacactttta ttaactcagc attattttta aacatcaaac cacttaatta
    14761 ttaacaaaat cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag
    14821 cgatttatga tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta
    14881 aaattcaaga catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt
    14941 ctttctctgc aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat
    15001 tgttttataa agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa
    15061 aaagcttctc agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg
    15121 tcattaaatt ttccacgtcc gtcttacaag ctgataagaa agaaattacg atgattaata
    15181 aaaacgaaaa cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg
    15241 aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccaa aaatcagata
    15301 gaaaagaaaa agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc
    15361 ccaaaaccgt atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa
    15421 tgttgattac aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt
    15481 caacaaaaaa tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg
    15541 cgttgctgcc tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc
    15601 aaatggcatc agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg
    15661 gctaagaata aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc
    15721 taatgtaact aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta
    15781 ttctctgatg gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt
    15841 tattaaccaa tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa
    15901 aggaactgtg gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc
    15961 aattggtcag aatcaccaac ttacgcaact gatgattcag ctccagaaaa tgccagaact
    16021 acatagaaca gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc
    16081 tataaattat ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg
    16141 atatgaaggg ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa
    16201 tggtttttct ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat
    16261 tctgaacgtc ttacctaaac tggccgcaac acatcattta gataatgaac aggtgtataa
    16321 attcctgagt gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg
    16381 tgatgccgac atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca
    16441 tttgactaaa gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag
    16501 actatacctg gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt
    16561 gccctgtctg gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc
    16621 taaaagtctt gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt
    16681 tattaatact atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa
    16741 aatatgaagc ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg
    16801 atacagcata aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca
    16861 cgcctgtttg gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa
    16921 aaatactctg cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc
    16981 cttaatacat taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt
    17041 gcggagaaaa attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa
    17101 atctttgcct gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt
    17161 gtagtgcgcg ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac
    17221 caatacgcgg gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg
    17281 agacgctgta ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt
    17341 cgcggaaatt cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc
    17401 cgtgacgggt gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg
    17461 caagctggcg aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca
    17521 cttgttccag ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt
    17581 acaatctgct caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt
    17641 aataccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc
    17701 attcggccca cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca
    17761 gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc
    17821 ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca
    17881 ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg
    17941 cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac
    18001 tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa
    18061 acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc
    18121 gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc
    18181 agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg
    18241 aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt
    18301 ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc
    18361 cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc
    18421 tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac
    18481 cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac
    18541 agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta
    18601 gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg
    18661 tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg
    18721 gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca
    18781 atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga
    18841 tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc
    18901 gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg
    18961 gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc
    19021 agcgcggtga ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct
    19081 gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc
    19141 ggctgctgca ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc
    19201 aggtcgaaca tgttaaacca catgctgtgg atacctgcta gggtgatgaa ctggaacttg
    19261 tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc
    19321 tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg
    19381 atagcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc
    19441 aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga
    19501 gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg
    19561 caatctgagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga
    19621 acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt
    19681 ggcactaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg
    19741 aagtgaactg ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg
    19801 acaccgccaa aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc
    19861 ggatcgcccg gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg
    19921 ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac
    19981 atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca
    20041 atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gttgttgatg
    20101 tagccttttt tcgactcacc gtgcagcaga cgccacattt tcgctgcgcc cagttgcgcc
    20161 agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc actgtatggg
    20221 cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc aatttgttgt
    20281 gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct aacaggcggt
    20341 agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt tccatcaagc
    20401 gtgcggcatc gtcaaaacgg ccctgggaga aacgttcttc gcccagttcg ctggcaatga
    20461 ctttcatctc ttcgcccagc atctggcgga acaaggcttt ggtcaccggt ttgccattgc
    20521 tcaacgtttt ttgatgatgg atccactgcc agatcgaggt acgggaaatt tcagccgtcg
    20581 ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc cacgcttcga
    20641 tgtactgcac agccacgcga atgttagcgc gcataccttc ttcggtgcgt tcaccatcac
    20701 aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcaat acttcaagct
    20761 gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtgtcc gcaaggcctg
    20821 ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta tccgctttta
    20881 ctttgttgag cacctggtta ttacgctctt catctttgct cggaataaac gcggccatgc
    20941 cgcccatcgc aaaagcaccg cgtttatggc aggttttaat cagcaggcgt gagtaagcat
    21001 tcaggaatgg tttatccatc gtcacggcct gtctgtctgg caggacacga tcgggatagt
    21061 ttttcaacgt tttgatatag ctgaagatgt aatcccagcg accgcagttc agaccaacaa
    21121 tatggtcgcg cagcgcgtga aggatttcgt ccatctggaa cacggcgggc agcgtttcaa
    21181 tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct gcatagctga
    21241 agacttcgct ccaccaggcc gcttcctgct aggactgggt tttcggcaga tagaaatagg
    21301 gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc gcaaaatcaa
    21361 acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc aagtgcagac
    21421 cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt ttgcctgctt
    21481 cattggtata gctgatggta ccgttaaccg catcgcgcag gttaatttgc ccgtcgatca
    21541 ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact ttcacattgg
    21601 cgttcagtgc gttgatcacc atcttgcgct ctaccgggcc agttatctct acgcggcggt
    21661 cttctaagtc cgcaggaatc ccgcgaattt tccaatcagc atcgcgaatg gaagccgttt
    21721 ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga atgcgcgctg
    21781 ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga aattctaccg
    21841 cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttgtgaaa gccagttcat
    21901 cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg aaaacttccc
    21961 ctctgtgaac gaaggaacgt tgtgcgcttt tcgttcagca caactaagac tactcattta
    22021 aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac ttattgataa
    22081 caatatgaat aaaatttatt cacttgctga ggtgcgtttc ggaaataaaa aaggcacccg
    22141 aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga agattaatcc
    22201 agcgttggat tcatgtgccg tagatcgtat ggcgtgatct gatagacgta atagttgagc
    22261 cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggttt gttttgcgga
    22321 tcgttgtgcg ggaaatagtt atacggtaca tccgggtcta gtccggcttc cacatcgcgt
    22381 aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac aaaggcaatg
    22441 cgcttatctt tgctggcaaa cagatatgca tccccttctt ccgtctctgc cagaatttcc
    22501 agatcggtgt aatcacgaat caacgctgcc ggaaagtcag catagcgcga atgcggtgcc
    22561 aggaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat atgatgctcg
    22621 taaacgccag agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag gatattgagc
    22681 gcggcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt cgaccactcc
    22741 agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag gcccagcggc
    22801 gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa gttacagtag
    22861 aagttgttca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat gcgcaacagc
    22921 tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt ttcagtttca
    22981 atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc ctgaccagac
    23041 gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac ggcgggtagc
    23101 tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt atacgtttag
    23161 acatccagat agctgaagat aaccagaaaa tttgccaatg tcgagcctgc atgttgaagg
    23221 tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa ggagagaaaa
    23281 tggttattag tattcgccgc tcacggcatg aggaagggga ggaactcgtt gcgatttggt
    23341 gtcgatctgt cgatgccacc cacgattttt tatcagcaga gtatcgggcc gagctggaag
    23401 agctggtgcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat gaacgggagc
    23461 agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt atcgatcctg
    23521 atgtgtgcgg ctgcggcgtg ggtcggatgc tggtggagca tgcgctctcg atggcaccgg
    23581 aactgacaac caacgttaat gagcaaaatg agcaggcggt tgggttctat aagaaggtgg
    23641 gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat ccgttgctga
    23701 atctggcgta tgtgggggaa taagccgcct ctgccgtgcc tgcccaagct ggagaagacg
//
LOCUS       DAJVQR010000060        23408 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVQR010000060 DAJVQR010000000
VERSION     DAJVQR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OQM52_002509"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OQM52_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1564318.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OQM52_002510"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OQM52_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1564319.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OQM52_002511"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OQM52_002511"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1564320.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OQM52_002512"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OQM52_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1564321.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OQM52_002513"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OQM52_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1564322.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OQM52_002514"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OQM52_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1564323.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OQM52_002515"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OQM52_002515"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1564324.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OQM52_002516"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OQM52_002516"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1564325.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OQM52_002517"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OQM52_002517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1564326.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OQM52_002518"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OQM52_002518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1564327.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OQM52_002519"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OQM52_002519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1564328.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OQM52_002520"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OQM52_002520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1564329.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OQM52_002521"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OQM52_002521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1564330.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OQM52_002522"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OQM52_002522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1564331.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OQM52_002523"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OQM52_002523"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1564332.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OQM52_002524"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OQM52_002524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1564333.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OQM52_002525"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OQM52_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1564334.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OQM52_002526"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OQM52_002526"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1564335.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="OQM52_002527"
     CDS             19508..20329
                     /locus_tag="OQM52_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS1564336.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="OQM52_002528"
     CDS             20316..22424
                     /locus_tag="OQM52_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS1564337.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..>23408
                     /locus_tag="OQM52_002529"
     CDS             22421..>23408
                     /locus_tag="OQM52_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1564338.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLS"
BASE COUNT         5929 a         5609 c         6093 g         5777 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcgg
//
LOCUS       DAJVQR010000061        22921 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVQR010000061 DAJVQR010000000
VERSION     DAJVQR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OQM52_002530"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OQM52_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1564339.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OQM52_002531"
     CDS             1207..1869
                     /locus_tag="OQM52_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1564340.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OQM52_002532"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OQM52_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1564341.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OQM52_002533"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OQM52_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OQM52_002534"
     CDS             2795..3100
                     /locus_tag="OQM52_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1564342.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OQM52_002535"
     CDS             3103..3507
                     /locus_tag="OQM52_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1564343.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OQM52_002536"
     CDS             3497..3796
                     /locus_tag="OQM52_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1564344.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OQM52_002537"
     CDS             3982..4278
                     /locus_tag="OQM52_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1564345.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OQM52_002538"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OQM52_002538"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS1564346.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OQM52_002539"
     CDS             5627..5995
                     /locus_tag="OQM52_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1564347.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OQM52_002540"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OQM52_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCS1564348.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="OQM52_002541"
     CDS             7203..7904
                     /locus_tag="OQM52_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1564349.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="OQM52_002542"
     CDS             7927..8091
                     /locus_tag="OQM52_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564350.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="OQM52_002543"
     CDS             complement(8303..9613)
                     /locus_tag="OQM52_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1564351.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="OQM52_002544"
     CDS             complement(9641..10972)
                     /locus_tag="OQM52_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1564352.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OQM52_002545"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OQM52_002545"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1564353.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="OQM52_002546"
     CDS             complement(12683..13072)
                     /locus_tag="OQM52_002546"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1564354.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OQM52_002547"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OQM52_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1564355.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OQM52_002548"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OQM52_002548"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1564356.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OQM52_002549"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OQM52_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1564357.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OQM52_002550"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OQM52_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1564358.1"
                     /translation="MHITYDLPVTIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OQM52_002551"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OQM52_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1564359.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="OQM52_002552"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="OQM52_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1564360.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="OQM52_002553"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="OQM52_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="OQM52_002554"
     CDS             21379..21726
                     /locus_tag="OQM52_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564361.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21895..22545
                     /locus_tag="OQM52_002555"
     CDS             21895..22545
                     /locus_tag="OQM52_002555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564362.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNYLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6185 a         5779 c         5281 g         5676 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agtaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattact tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
    22921 g
//
LOCUS       DAJVQR010000062        22538 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVQR010000062 DAJVQR010000000
VERSION     DAJVQR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            16..225
                     /gene="fumD"
                     /locus_tag="OQM52_002556"
     CDS             16..225
                     /gene="fumD"
                     /locus_tag="OQM52_002556"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1564363.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            681..1307
                     /locus_tag="OQM52_002557"
     CDS             681..1307
                     /locus_tag="OQM52_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1564364.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1328..3429
                     /locus_tag="OQM52_002558"
                     /pseudo
     CDS             1328..3429
                     /locus_tag="OQM52_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3442..4080
                     /locus_tag="OQM52_002559"
     CDS             3442..4080
                     /locus_tag="OQM52_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1564365.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4144..4812
                     /locus_tag="OQM52_002560"
     CDS             4144..4812
                     /locus_tag="OQM52_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1564366.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4809..5594
                     /locus_tag="OQM52_002561"
     CDS             4809..5594
                     /locus_tag="OQM52_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1564367.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5598..6410
                     /locus_tag="OQM52_002562"
     CDS             5598..6410
                     /locus_tag="OQM52_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564368.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6422..7844)
                     /locus_tag="OQM52_002563"
                     /pseudo
     CDS             complement(6422..7844)
                     /locus_tag="OQM52_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7970..8275)
                     /locus_tag="OQM52_002564"
     CDS             complement(7970..8275)
                     /locus_tag="OQM52_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1564369.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8383..8459)
                     /locus_tag="OQM52_002565"
     tRNA            complement(8383..8459)
                     /locus_tag="OQM52_002565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8423..8425),aa:Val,seq:gac)
     gene            complement(8464..8540)
                     /locus_tag="OQM52_002566"
     tRNA            complement(8464..8540)
                     /locus_tag="OQM52_002566"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8504..8506),aa:Val,seq:gac)
     gene            8848..10104
                     /locus_tag="OQM52_002567"
     CDS             8848..10104
                     /locus_tag="OQM52_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1564370.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10145..11518)
                     /gene="mdtK"
                     /locus_tag="OQM52_002568"
     CDS             complement(10145..11518)
                     /gene="mdtK"
                     /locus_tag="OQM52_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1564371.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11733..12374
                     /gene="ribE"
                     /locus_tag="OQM52_002569"
     CDS             11733..12374
                     /gene="ribE"
                     /locus_tag="OQM52_002569"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1564372.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12414..13562)
                     /gene="cfa"
                     /locus_tag="OQM52_002570"
     CDS             complement(12414..13562)
                     /gene="cfa"
                     /locus_tag="OQM52_002570"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1564373.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13853..15064)
                     /locus_tag="OQM52_002571"
     CDS             complement(13853..15064)
                     /locus_tag="OQM52_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1564374.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15177..16109
                     /locus_tag="OQM52_002572"
     CDS             15177..16109
                     /locus_tag="OQM52_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1564375.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(16106..17131)
                     /gene="purR"
                     /locus_tag="OQM52_002573"
     CDS             complement(16106..17131)
                     /gene="purR"
                     /locus_tag="OQM52_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1564376.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17430..17519
                     /gene="ynhF"
                     /locus_tag="OQM52_002574"
     CDS             17430..17519
                     /gene="ynhF"
                     /locus_tag="OQM52_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1564377.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17685..18854
                     /locus_tag="OQM52_002575"
     CDS             17685..18854
                     /locus_tag="OQM52_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564378.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19000..19581)
                     /gene="sodB"
                     /locus_tag="OQM52_002576"
     CDS             complement(19000..19581)
                     /gene="sodB"
                     /locus_tag="OQM52_002576"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1564379.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19709..20536)
                     /gene="mepH"
                     /locus_tag="OQM52_002577"
     CDS             complement(19709..20536)
                     /gene="mepH"
                     /locus_tag="OQM52_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1564380.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20871..21218
                     /gene="grxD"
                     /locus_tag="OQM52_002578"
     CDS             20871..21218
                     /gene="grxD"
                     /locus_tag="OQM52_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1564381.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21257..21757)
                     /gene="tnpA"
                     /locus_tag="OQM52_002579"
     CDS             complement(21257..21757)
                     /gene="tnpA"
                     /locus_tag="OQM52_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1564382.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21765..>22538
                     /locus_tag="OQM52_002580"
     CDS             21765..>22538
                     /locus_tag="OQM52_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1564383.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVS"
BASE COUNT         5852 a         5247 c         5829 g         5610 t
ORIGIN      
        1 aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg agaaatgtgc
       61 agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc aaagcatatt
      121 gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg cagtgagctg
      181 gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt caggatgagg
      241 cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca ttctaaataa
      301 caactgctga aattatacga atttcggtat gtattttaat ttatagcgat aatgttattt
      361 ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta tttcaaatga
      421 taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat aacgatcaat
      481 gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat tagtttatcg
      541 gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt tggggacaga
      601 gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag cactgtgttt
      661 gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat attggcttaa
      721 cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca attgcccctg
      781 cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca gtagggttga
      841 taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt tgccaccgct
      901 gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc tcccgtatca
      961 aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc ggcctgtatg
     1021 gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa tgcatgaacg
     1081 tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc gtcgatcata
     1141 aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg gccaccgtaa
     1201 ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca aacgcctgcc
     1261 caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc ttgcaaagga
     1321 aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca cgacaggaaa
     1381 tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg gcttcggcta
     1441 caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag cgaataaatt
     1501 agtctttgct accggcccat tacctggatc tggtgccccc tgtagttctc gcgtaaatat
     1561 cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat gggtggcttt
     1621 tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg gaaggcgaaa
     1681 tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc cgatttctta
     1741 tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag cccggaaacc
     1801 tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg catgttgaat
     1861 agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa aaacctgaaa
     1921 gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga gatgaagcgt
     1981 ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt cgtgccaagt
     2041 acgccacaat cgtgggcaga gtattcagat cccaagtcac gctggacagc acgtaaaggg
     2101 ctgttttggg gcgcggctga aggtggtccg attgaaacgg gtgaaattcc gccaggcaat
     2161 cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggacc ggcggcagag
     2221 aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat gacccaaatg
     2281 aatattcctc gggtgaaaga gtttggcgtg cccagcacag gtggtaacac ttgtgtagca
     2341 aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga taaagacgat
     2401 ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg cctatggtgt
     2461 aactacgggc agttgcatcg cgactttact tattgttaca gcaaaggtgt gttcaagcgt
     2521 gttctgccag ctgaagagta tgcagaaatt cgctgggatc aactggaagc gggtgacgtt
     2581 aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct gagtcacctg
     2641 gctgatggtt catatgccat cgcagaacgc tggaatttgg gtaaagagta ctggggctac
     2701 gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa tgaagcgtca
     2761 gcgcaggtcg gttccattgt taagtgtatg ttcaaccgtg actgcatgac gcatacccat
     2821 atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc gaaagaactt
     2881 tttggttctg aagatgctta cgatgaaacc aaaaactaca cgccaatcaa tgacgcaaaa
     2941 attaaatatg caaagtggtc gctgttgcgg gtctgtttgc ataacgccgt cactctgtgc
     3001 aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg gggcgatctg
     3061 gcgcttgaag ccaaattctt caaagcgatc accggcgaag agatgactca ggaaaaatta
     3121 gatttagctg cagagcgtat ctttacgttg catcgtgcct acacggtaaa accgatgcaa
     3181 accaaagata tgcgtaatga acacgatctt atctgttcct gggttttcga caaggatccg
     3241 cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat gcatgcttcg
     3301 ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgtcc aacccgcgaa
     3361 acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca caatctactg
     3421 cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc gaaagtcagc
     3481 gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt tgatacggta
     3541 accgaaccag aacctgctga aatcctggct gagttcattc gccagcattc tgcggctggg
     3601 cagctggtgg cgcgcaccgt gtttctctcg ccaccctatt cggttgcaga agaggaactg
     3661 tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc ttgtatgacg
     3721 ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta cgcggcgatg
     3781 tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt tcgttttgag
     3841 tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc atattacttc
     3901 caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga atatgccgat
     3961 atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg atttatgacc
     4021 tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca gaacccctga
     4081 aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt aggggataat
     4141 cagatgtcct tcactcgacg caaatttgtt ctggggatgg gaacagtaat cttttttact
     4201 ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg gtatgccatg
     4261 attcatgacg agtcacggtg caatggctgt aatatttgcg cccgcgcatg ccgtaaaact
     4321 aaccacgtcc ccgctcaggg aagccgttta tcgatagcac acattccagt caccgataac
     4381 gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga agatgctccg
     4441 tgcatcgacg tatgcccgac gggggcatcg tggcgtgatg agcaggggat cgtgcgggta
     4501 gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta cctggttcgc
     4561 taccttaacc ctgtcactaa agtggcagat aaatgtgatt tctgcgctga gtcccgactg
     4621 gcaaaaggct ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt gatctttggt
     4681 cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta tcaatatcag
     4741 ctacctggtg ccggaaaacc gcatctgtat cgccggttcg gtcaacattt gattaaaaag
     4801 gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta gcgaactatg
     4861 tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga gtattgctgg
     4921 ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc gtgaagaaat
     4981 cagccaccga tcatggtgag aaggtttatc tttacagcaa agcggtcaga ttatggcact
     5041 ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat cactttgcga
     5101 tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc ggatttttgt
     5161 tactggcatg ctggctcggc tttgtgctga tcaatgccgt tggggataat ggtcaccact
     5221 atcgcattcg tcgtcagggg tggctggaac gagcggcaaa acaaacgcga ttttatttgt
     5281 ttggcattat gcagggggaa gaacatcctt tcccggcaac aacacagtct aaatttaatc
     5341 ccttacagca ggtcgcctat gttggtgtca tgtatggatt gctgccgttg ttactattga
     5401 cggggctgtt gtgtctctat ccgcaagccg tgggagatgt gtttcctggc gtaagatact
     5461 ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc ttcggtcatc
     5521 tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg gtcgatggct
     5581 atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg cgtatcggag
     5641 cggtgatgta tcgctggttc ctgcgccatt tcccacgcgg cggtagttat gccgatattc
     5701 gtcatgcact gattgaggaa ggatatacgg actgggcgga gagtctggtt gagtatgcgt
     5761 ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct atgcaaaaac
     5821 tggccacaga ccccggagag ataccatttt gcagtcagtt tgcccgcagc gacgatcatg
     5881 ctcgcatcgg ctgttgtgag gataacgccc gaatcgcaac cgctgggtat gccgcgcaaa
     5941 ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt cacattggca
     6001 gctccggtga acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata agcagcgctg
     6061 gagatagtag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca ttgggtgaac
     6121 ggtgtcatgt cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc gcaaacgcca
     6181 gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat tcaacggtag
     6241 tgagcacggg cgtggtcgac tcaattattc tcgggctagg gggaagcgcc gcactggcat
     6301 atcatgacgg cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac aatattcgaa
     6361 ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga tagctttacc
     6421 tttaatcaaa cgaaagacgc cgacgagcac gggatgcagg ctttacgacc gcccgagcca
     6481 tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg tcgtgcatca
     6541 accagggcaa cgcgccgaac gctacgcgtc cacgaatatc ttcgggttgc tgtaacgtat
     6601 aatcttcgcc aacatcaggg atttcagatc cccgcttcac gtaaggccag tagtcgacga
     6661 atagactctg aagggattgc cgcataatta tcgatgaaaa ccctggcaag gcctttactg
     6721 aaccgtttat ggtcgtgttc attcagatga ggaacaattt cctgtacggc ttcatgcagg
     6781 cgcagaatga cataacgcca gggaacagta tgtttttctc gtttattgcg ttcgacttcc
     6841 tggagatttt tttctgccca gtcaaatggg tcgcgttgtt tgcgctcggc aaaccaggct
     6901 tgagcaaagg aatcgacatt caggctctct aaggctatgc gttggctcca gtctggatca
     6961 gcaaacttga tttcctctac tatcaatcta aatacccgat ccaaaaggcc ctcttcacct
     7021 ttttgaatct cagcatttaa tgcctgatcg gtgacgatgt gtaagggctc gtagggaata
     7081 gggcaataga aatcggcttc gggtaaaata cccgtgcgcg acatcaacgt aatatttagc
     7141 ttttcacttg cgttatcgcg gtgaaaaacg acgtgttgtt tatcatcttc aatgaacgaa
     7201 ccatgctgaa tagccactgc cattgccgca tccagtccgc tcaaggatgt tcccataata
     7261 cccacgttac acgcatcgac ctttgcttcc atcaggcctg accacgggct gggaaaatac
     7321 gttctggttg cttcttcttc atcaggccag acgtgacccg tggcaatcac cgctaaatca
     7381 aacgtctcgc tgggtaaatc ctgatttgta gcgagcatga cgccagcatt tgtaatttgc
     7441 agatcggtaa cctggcatga ttcataaaca gccactgcaa atttttgctg tcgtgcctgg
     7501 tctactaatc gtaaaaattg atcgcggaaa tattcgccca gcagaattcg cggtaaaaac
     7561 tgacgatcgt gcaaggtttc ttttttaacg ccataacgct ggagatggct ggcttcttgc
     7621 ttttgtagcc attcgagata cgtacaatta atcggcggta tttcaatact ggcaatattt
     7681 gccagcatca tttttgagtt ttcctcatca ctgtatggca tcccgacacc ggcctcgtca
     7741 gcctgctcga agatagaaat agaaagtgga gtttgttgct gtagaagcga gaataaggtg
     7801 tagatccccg taggcccggc acccacaata gcaatttttt tcattgttac ttacctgtac
     7861 gcattctttc gaatttagtg ttatcagata agtttggttg agatttttag aatttgagaa
     7921 atttcagaca gggatgaaaa gggcgctaac cgcccttgat taaattctgt caggcgagtt
     7981 ttgcctgatt gatttgacta agtggctcat tgacatcgaa aactttggcg acaacttcct
     8041 caacgccgag atttttcagt cgggcagtat gtttttccag ataggcaagg gcgctttttt
     8101 catcagtgaa aaggtagatc ccaccggctt cgtggttctt ttcactttct gtccatacct
     8161 tccacagaaa accaggttcc tgattaatcg actcagcaag tggtttaagc tgctcagcca
     8221 ttgcgtcgcc aaatgggcca ttaaaagcaa aatgaagttg taacaaagtt gccatagcga
     8281 ttctccttct gataacgcat acgaagacag tgtaaatctt ttaacgtatt ggatacagag
     8341 aagaaacaga gggaaaaagg aaaagaccag attaagaaaa tctggtgcgt ccgagtggac
     8401 tcgaaccacc gacccccacc atgtcaaggt ggtgctctaa ccaactgagc tacggacgca
     8461 ggatggtgcg ttcaattgga ctcgaaccaa cgacccccac catgtcaagg tggtgctcta
     8521 accaactgag ctatgaacgc aacgttgtag gtgacaacgg ggacgaatat tagcggcaga
     8581 gtggaaaggt ggcaagaggc aaaacgtaat tttctgcgct atttcgaccg tttgcagagc
     8641 ttttaagcaa attggctata ttttgttgat ttgcaagggc gatttttatt caggatcgca
     8701 tttacatctg atacaaccca ataattcgtg tatcagagtt taaacagagt tgtttgccac
     8761 catctcaaat ctgtcgtgga taaatcctcc gtagccttta cacttaggta gcaataaagg
     8821 cacacagatg cagcaaagga ataaatcatg ggatctgatg cgaaaaactt gatgagcgac
     8881 gggaatgtgc aaattgttaa gaccggcgag gtccttggcg cgacgcaact tactgaaggc
     8941 gagttgattg ttgaagctgg cggaagagcc gaaaataccg tggtcacggg ggctggctgg
     9001 ttgaaagtgg caaccggtgg gatcgccaaa tgcacacagt acggtaacaa tggcacgcta
     9061 tcggtcagcg acggtgccat tgccacagat attgttcagt ccgagggagg cgcaattagt
     9121 ctctctacgc tcgctacggt taatggccgc catcccgaag gtgaattcag cgttgatcag
     9181 ggttatgcct gcggtttgtt gctggaaaat ggcggtaacc tgcgtgtact ggaaggacat
     9241 cgcgcggaaa aaatcattct cgatcaagag ggcggcctgt tggttaatgg gacaacctca
     9301 gcggtcgtgg tagatgaagg tggtgaattg ttggtgtatc caagtggaga agccagcaat
     9361 tgtgagatta atcagggcgg cgtttttatg ctggccggga aagccagtga tacgttgctt
     9421 gctggtggca ccatgaataa tctcggtggt gaagactctg acactattgt tgagaatgga
     9481 tccatttatc gtctggggac ggatggcctt cagctctaca gttccggtaa gacgcaaaac
     9541 ctgtcggtga atgtgggggg gcgggctgaa gtgcatgccg gtacgctgga aaatgcggta
     9601 atacaaggtg gaacagtgat cctgttgtca cccaccagcg cggacgaaaa ttttgtcgta
     9661 gaggaagatc gcgcaccggt tgaactgacc gggagtgttg cattactgga cggcgcttca
     9721 atgattattg gttatggcgc agagctgcaa caatcaacga ttactgtaca gcagggcggt
     9781 gtgttgattc tcgacggcag tacggtaaaa ggtgacagcg tcactttcat tgttggtaac
     9841 atcaatctga atggcggaaa actgtggctg atcactggtg cggcaacgca tgtgcaactg
     9901 aaagtgaaac gcctgcgcgg agagggagcg atttgcctgc aaaccagtgc gaaagaaatt
     9961 tcacctgact tcatcaatgt gaaaggggaa gttaacgggg atatacgcgt tgagataaca
    10021 gatgccagtc ggcaaactct gtgtaacgct ctgaaattac agccagacga agacgggatt
    10081 ggcgcaacgc tccagcctgc gtaaaaacac aggcgcaacc cgtgaaggtt gcgccttgtc
    10141 tttattagcg ggacgctcgt tgcagaatga tggctgacgg catacgttgc aggaaccgca
    10201 tacgcagcat catcataatg gctgccgatg tcaggccgat aataaagcct atccagaagc
    10261 ctgctggccc cataggttca acgaccagat cggtcagtgc cagaatatag ccgcttggca
    10321 agcccagcac ccagtaagct gtaaaggtga tatagaaaat ggaacgcgta tctttataac
    10381 cacgcaaaat cccactgcca atcacctgga ttgagtcaga aatctgatat accgccgcca
    10441 gcaacatcaa atgcgcagcc agcgttacaa cctcgggatt gtcgttgtac aacagggcga
    10501 tttgctcccg cagtgaaacc gtgaaaatgg ccgtcagggt tgccatacag acacccacca
    10561 taagcccggt ccgcgcagcg gtttgcgcat ccagcgttga gccctgaccc agacgataac
    10621 ctacgcggat agttaccgct gccgccagcg acattggaag cacaaacatc agtgaactaa
    10681 agttcagggc aatctggtgt cctgcgacat caacaatacc gagcggagac actaacagag
    10741 ccacgacggc aaacagtgtc acttcaaaga acagtgccag cgcaatcggc aaaccgagtt
    10801 gaatcagtcg tttcataacc gcaggctcgg gcttagcggt gcctttttcg ttacgaatat
    10861 cgcgcatgga gcgggcgcgt ttaatgtaag aaaccatggc aaggaacatg acccaataca
    10921 ccgccgcagt agccacgcca caaccaacgc caccgagctc aggcataccg aaatgaccat
    10981 aaataaagat atagttcacc gggatgttca ccagcaggcc gataaagccc attaccatac
    11041 ccggcttggt ttttgccaga ccttcacatt ggttacgggc aacctggaag aacagatatc
    11101 ccggcgcgcc ccacaacaac gcacgcagat aacccacggc tttgtccgcc agagccggat
    11161 cgatgttttc catgtagcgg ataatgtaac ctgcattcca cagcaccagc ataatgagaa
    11221 cggaaacaaa acctgccagc cagaaacctt gtcgcacctg atgcgcaatg cgctcacgtc
    11281 gaccggaacc atttaattgc gcgataaccg gcgttaatgc cagcagcagt ccgtgaccaa
    11341 agaggatcgc cggaagccag atagaagtac cgatagcgac cgccgccatg tcggtggcac
    11401 tatagccgcc cgccatcacg gtatcgacaa aacccatcgc agtttgggcg atttgcgcga
    11461 gaatcaccgg gattgctaat gctaataaca gacgcgcttc actgatatac ttctgcacgt
    11521 gaacaccttt tatttgtagt tatatgagag actaaaaagc cgcctgaatg ggcagcaaaa
    11581 agaagaagca ggggaaattc agtctattgt agcgaggatt tattaattct ccagtgaaaa
    11641 aatagccatt cttacccctt cactggtaag ggcgttttcc acctgctatt gtgctgaaca
    11701 gaatgtctta actgatttca ggagttgtaa gtatgtttac ggggattgta cagggcaccg
    11761 caaaactggt gtcgattgac gagaaaccaa attttcgtac gcatgtggtg gagttacccg
    11821 accacatgct ggacggcctg gaaaccggtg cttccgtggc gcataacggt tgctgcctga
    11881 ccgtgacgga aattaacggc aaccatgtca gttttgacct gatgaaagaa acgttacgca
    11941 ttaccaatct tggcgattta aaagtggggg attgggtaaa cgttgagcgt gcggcgaaat
    12001 tcagtgatga aattggcgga cacttaatgt caggtcatat tatgaccacc gctgaagtgg
    12061 cgaaaatatt aacctcggaa aataatcgcc agatctggtt taaagtccag gatagtcagt
    12121 tgatgaaata tatcctgtac aaaggattta ttggcatcga cggtattagc ctgaccgtcg
    12181 gcgaagtcac gccaacgcgt ttttgcgtcc atttaattcc ggaaacactg gaacgcacga
    12241 ctcttgggaa gaaaaaactt ggcgcacgcg tcaacattga aatcgatcca caaactcagg
    12301 cagtggtaga tacggtagaa cgtgtgctgg cggcacgaga aaatgccatg aatcaaccag
    12361 gtacagaagc ctgatctgaa ctcgcccccg aagagggggc gatagaatag cctttagcga
    12421 gcgactcgaa ggccgttttc aacaccgcgt gagaacacca cctgccagag ctgaatatca
    12481 cgggcgcgga aagcacctgc gcaggcattc agataatagg taaacatccg tttaaagcgt
    12541 tcactgtagt tatccgcaat ttctggccat gcggcgagga atcgttcata ccacgccatc
    12601 aacgtagtat cgtaatcagc accgaagtta tgccagtctt ccatcacaaa gtggggttcg
    12661 ctggattgag caatctggcg tacagagggc aggcaaccgt tcggaaaaat atatttatta
    12721 atccagggat caacattcag atcggttttt ttcgaaccga tagtatggag caggaatata
    12781 ccttccggtt tcaaattacg atccaccacc gcaaaatagg tatcgtaatt tttcggtccg
    12841 acgtgctcga acatccccac agaaacaata cgatcaaact ggtcgttcag gtcacgataa
    12901 tcttgcagca aaatggtgac atccaggcct ttacagcgtt cctgagccat tttttgctgt
    12961 tcggcagaaa tggtgacgcc gaccacgctt acgtcatagt tagatgccat gtagtgcgct
    13021 agtccgcccc agccgcagcc aatatccagt acgcgcatcc ctggttttaa ctgcaatttt
    13081 tcacaaatca ttttgagctt cgcctgctgg gcagattcca gattatcggc atctttccag
    13141 taagcgcagg aatattgcat gaagggatca agcatgcggc tgaacaagtc attacccaaa
    13201 tcgtaatgct ccttgccgac tatccaggca cgttttttac tctgcagatt gaagagacga
    13261 gcgccggcaa tacgcagcgt gtctttgaaa tgatggggga gttggttctc gagacctgcg
    13321 cgtaagactt tgctaaaaaa catatccagt cggtcacatt cccaccagcc atccatataa
    13381 ctttcgccta accccaaaga gccttcctgc agaacgcgtt taaaaaaatc ggggtttttc
    13441 acacgaatat ccgccggggc agaaccgtta atggctatac cggcacggct aagtaattcg
    13501 ttggcgatac ggtaccagtt gtcatccggt acactgactt cttctataca cgatgaactc
    13561 atagtttctc catcactggt cggtgatcag aaccttaaaa cagcgtagac gctttttggg
    13621 ctttgtgaga aatctcgcag aaaaaaccgc gagtctataa tccgacgtag aacagaggga
    13681 aaggaggaac ccttgccgaa atgaccatcc atagtgagtc gggaacgatc ctgtccccgt
    13741 aaaaacataa tatttatcgt agctttaaca actcgaaatt agtataggcc gctaatttca
    13801 atcatacaac aaaattttgt tagcagccta agtataagta tatcgatata gatcagtgtg
    13861 attcgctatg agcgacttcg gcattgccat gattcgggca gtcaacttct tcacaacgtt
    13921 gcatcatgta acccagcgcg accagcacta ctgttgataa catcacgctg gtggtggtga
    13981 gcaatggcgt gctgatacta ataagccagg aaactaccag acttgcgagg aagcacagac
    14041 ccagttgcag agtgttctgc aacgctgcgg cgcgaccggt tgcatgtggg aagggacgca
    14101 gcgcctgggc gacaacaata gggtagatcg cgccattggc aatcgccatc acacagaatg
    14161 ggatcaggat ttcgaccagc gacacatggc taatgaagcc cgcagcccag gtcgcaatga
    14221 cgctgacagc aaacagcacc agcaaccacg gtaataactg cttgccttgc catttctgca
    14281 gtgcggcgcg acagccataa ccaccaatca gaaacgcgat agtttgcggg acataactta
    14341 aaccaataac tgccgggctg tagcccattt cactaaggat gaacggtgaa ccggtcagcc
    14401 atgcaaaaaa actggctgaa caggctgcgt atatcagcac gttgccgcga taggttttag
    14461 aacgtagcag gtcggtaaag gtcagaccat cctgactatt gttacgggcc ttcgtcgtgg
    14521 gtttgagcca gaaaataggc agaatcagca ccacggtaat ggcaaacagg gtggcgaaaa
    14581 tcgcctgcca ggaaaaatgg accagcagcc agcttcctaa caggggagcc agtgccggag
    14641 acagacccac cagcggcatg atggtcgcaa aaatacggtt gactttctgt gaaggataat
    14701 aatctgtcac taatgcttgc cagataaccg ccgcggcgca gacccccaca gcctgtacaa
    14761 aacgcaatac cagcagcgtg gcggcgtttt ctacccacag catccccaga ctacctaacg
    14821 caaaaattgt caggccgatt aataacaccg gtttacgacc ataacggtcg gagagtggcc
    14881 cccacagaag ctgggctgcg gcaaaaccgg caaggaacag actaaggctg gcactgacag
    14941 cagacgcagg cgtttgcagg tcggcctgta tggcggcgaa agcaggcaga tacatatcgg
    15001 ttgccagaaa accgagtacg ctcaaacccg ccagccagac taaaaatctt ttcccaggtt
    15061 gcatgtatat tctctaaatc tctcaattaa acaccgccgc aaagtgtagg gagtgaaatt
    15121 agtgttgtga aacggtaata tttgatgact ggtttcaaaa attttgcagg cagactatgt
    15181 ggtcagaata ttcactcgaa gttgttgatg cggtagcgcg taatggtagt tttagcgctg
    15241 cggcacagga gctgcatcgt gttccttctg cggtcagcta taccgtgcgt cagctggaag
    15301 agtggctggc ggtaccgctc tttgaacgac ggcaccgtga tgtggagctg accgctgctg
    15361 gagcgtggtt tctcaaagaa gggcgctctg ttgtcaaaaa aatgcagatc acccgccagc
    15421 aatgtcagca gatagcgaac ggctggcgcg gtcagttagc tatcgcagtg gataatattg
    15481 tcaggccaga acgtacacgg cagatgatcg ttgattttta tcgccatttt gatgatgtcg
    15541 aacttcttgt ctttcaggaa gtgttcaacg gtgtctggga tgcgctttcc gacgggcgcg
    15601 tggaactggc aattggcgca acacgcgcga ttccggtagg cggtcgttat gccttccggg
    15661 atatggggat gctaagctgg agttgcgttg ttgccagtca ccacccgctg gcgttgatgg
    15721 atggcccgtt cagcgatgat acgttgcgca actggccgtc gttggtgcgc gaagacacct
    15781 cgcgaacgct acctaaacgt attacctggt tgctggataa ccaaaaaaga cttgtcgtac
    15841 ctgactggga atcatcggca acctgtatct cggcaggatt atgcataggg atggtgccaa
    15901 cacatttcgc caaaccgtgg cttaatgagg ggaagtgggt agcacttgaa ctggaaaacc
    15961 ccttcccgga ttcggcatgt tgtctgacat ggcagcaaaa tgatatgtcg cctgcgttaa
    16021 cctggctgct tgagtatctc agcgatagtg agacgctgaa taaggagtgg ctgcgggagc
    16081 cggaagagac tcccgcaacg ggtgattaac gacgatagtc gcggaacggg ccgtcagcca
    16141 cggagcggcg ttcaatcaaa cgcggatgca cttcaatgga ctgcggttct tcacgtttgt
    16201 tgacgatacg atccaacagc atgttgaacg ctgtttcacc cagcgaatct tttggctgat
    16261 ggatcgtggt cagcgccggc gtaaaatagc gcgcgttgcg cacgttatca taaccgatca
    16321 gcgaaacatc ctgcgggacg cgcaggccca tttcatcagc agcacaaagt gcgcccattg
    16381 ccatgatatc gccaccacag aagacggcag tagggcgatg cggctgcgac aggatttgct
    16441 gcatggcgcg ataaccggat tcaggttcaa agtcaccctg cacaatccag ctttccggca
    16501 ccttgatcat cgcttcttcc atcgccttca taaaaccggc aaggcggcct gcgccggtgt
    16561 tacgttccag cgggccgggg atgacgccga tttcgcggtg accgcgttca atcagataac
    16621 gcccggccat gtagccgcct tcgaacgcgt tatcaatgac cgcatcggtg aagtcagctt
    16681 ttgcttcacc ccagtccatc accaccattg ggatatggcg atactcttcc agcatcgcca
    16741 gcaacggctc tgggtactca gaacacatca ccagcagacc atcgacgcgt ttttgcgcca
    16801 tcatcgacag ataagcccgc tgtttctcaa gattgttcca cgcattaccc agaatcaggg
    16861 tgtaaccttt ctggaagcaa tttttttcaa ctgcttcaat gatctcggca aaataggccg
    16921 cttcgctgct ggtcgccagc aaaccgatag acttggtgtg gttaaccttc aggctacgcg
    16981 ccaccgcgct aggggagtag tgtaattctt taatcgctgc ccacacggcg ttgcgcgttt
    17041 cttcagcgac gaaacgtgtt ttgttgatca cgtgtgacac agttgtagtg gaaacgtttg
    17101 ctcgtttcgc tacatctttt attgttgcca ttccatttca ctccagaccc taacttcagc
    17161 tcctgcaaaa tcgcaaggta aacgtttgcc tttacacacc ttacgcacaa caaaagtgtt
    17221 gcggtacgcc ggaaaaacga cacggagcgt caggaagggg tcaatggcca gtacgcaaat
    17281 agtgtaagcg tggaattttg gccgatcttg acgaaaaggg gaagtggtaa aaacacttat
    17341 cattataaat gacgggagat tttttcatca cagtgtgtaa aaatgcgtaa tatcactttt
    17401 tgtgtggtaa taaaaaagga gaaaacttga tgagcaccga ccttaaattt tcactggtaa
    17461 caacggttat cgtcctcggt ttgatcgtag ccgtgggttt gactgccgcg ctgcactgat
    17521 ttcctctcga gggagcgcgt tctccctcgc atctccttcc tgtttgttag caaaatggca
    17581 aaagtgtttt ctctttttgt tattttcatt tttttgtgat tgctgtcatg ctttcgccac
    17641 tttaatgaat atcgcgtaat gacgcgaaga ggtggagata aggcatgaaa attaactatc
    17701 cgttgctggc gctggcgatt ggcgcgtttg gtatcgggac aacggagttc tcgccaatgg
    17761 gcttgttgcc cgtcattgcg cgcggtgtgg atgtctcgat tcccgctgcc ggaatgttaa
    17821 tcagtgccta tgcagttggc gtaatggtgg gcgcgccgct gatgacgctt ctactttctc
    17881 atcgtgcccg ccgcagtgcg ttgattttcc tgatggcaat tttcacgcta ggcaacgttc
    17941 tttccgccat cgcgccggat tatatgaccc tgatgctttc acgtattttg accagcctga
    18001 atcacggagc attttttggt ttgggttcag tcgtggccgc aagcgtggtg ccaaaacata
    18061 aacaggccag cgcagttgcc actatgttta tggggttaac cctggcaaat atcggtggcg
    18121 tgccggcggc gacctggttg ggtgaaacca tcggctggcg gatgtcattt ctggcaacgg
    18181 cggggctggg agtgatttca atggtaagtc tgttcttctc attacctaaa ggtggtacag
    18241 gggcacgacc tgaagtgaaa aaagagctgg cggtattaat gcgtccgcag gtgctgtctg
    18301 cattgctgac gacggtactg ggagctggtg caatgtttac cctctacacc tatatctctc
    18361 cggtactgca aagtattacc cacgcaacac cggtgttcgt cacggcaatg ctggtgctga
    18421 ttggtgtcgg attctctatc ggtaactatc tcggcggcaa actggcagat cgttcagtta
    18481 acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat tatgctggca atcccgttcc
    18541 tggcccgcaa tgagttcggc gcagctatta gcatggtggt gtggggcgca gcaacctttg
    18601 cggttgtacc tccgttacag atgcgcgtga tgcatgtcgc cagtgaagcg ccaggtctgt
    18661 cttcatcagt caatattggt gcctttaatc ttggaaatgc gctgggagca gctgctggtg
    18721 gtgcggtaat ttccgctggg ctgggataca gctttgtgcc ggtgacgggg gcgattgtcg
    18781 cgggactggc attattgctg gtgtttatgt cagccagaaa acaacctgaa acagtttgcg
    18841 ttgctaacag ctaattaaac aaaaacgcag aaggataacc ttctgcgtta cagttgctga
    18901 caacgtgcga tttatcttgc cgaatgcgct gttaacgcct catccgcgtt ggcatggtaa
    18961 gcgaagcgca tcaggcaatg ctgcatttgc catcagttat tatgcagcga gatttttcgc
    19021 tacgaattcc cagttcacca gcgcccagaa gtgctccaga tagccaggac gtgcattgcg
    19081 atagtcgatg taataagcgt gttcccagac atcaacggtc agcagcggag tcgcatcggt
    19141 ggtcagcgga gtacccgcgt tagaggttga aacgatagcc agtttgccat cgctgttttt
    19201 caccagccag gtccagccag aaccaaagtt tttgatcgct gcatcagtaa actgcgcttt
    19261 gaaatcggca aagctgccaa aagatgcggc gatagcttca gcgacttttc cagtcggttc
    19321 gccaccggcg ttcggtgcca ggcagttcca gtagaaagta tggttccaga cctgagctgc
    19381 gttgttgaat acgccacctt cagagctgcg aataatctct tccagtgatt taccttcaaa
    19441 cgcggtacct ttaatcaggt tgttcaggtt agtgacataa gtctgatgat gcttgccgta
    19501 gtgatactcg atagtttccg cagaaatgtg cggtgccaga gcatctttag catatggtag
    19561 tgcaggtaat tcgaatgaca ttgctactct cctttattat taatttgcat acgtacaata
    19621 gccttattgt gcgtatggat agggtagcaa attaaaaggt gagacaaaag atgaacttac
    19681 cctgttgccg taacaacagg gtaaagtttt agcgaagtgt ttttggggtc attacccgac
    19741 gagcgccaac atagtggcgc tgccagtagt cttcactgag agaagtgatt tgaatttcct
    19801 gacctgtgcg cggtgactga ataaatttgc cgttgccgac atacacgccg acatgatcgg
    19861 ctgtaccgcg tccctgagta cggaaaaaga ccaggtcgcc gtttttcagc tcactacgtt
    19921 cgataggtcc tgcatcacgc aggtgataca tttcattcgc cgtacgcgga atacgaattt
    19981 tcaccaaatc tttataagcg taataaacca ggccgctgca atcaaaaccg gtacgcggtg
    20041 agctgccgcc ccaacgatat ggcttaccaa tttgctgcat cagtttattc attgccactt
    20101 tcgtggcttt ttgcaccttc gctttgtgcg cgtcggcaag ggttcctgac gcggcatttt
    20161 tgactttcac acaatgcgat ttatagccct tacgtttggt acacttttcg gtaaagctgg
    20221 ctgaggccgt tttattgacg gcgtgtttgc tgcgagtttt ggcgttttta gaagcggtaa
    20281 tggaagattt tttaacagaa gaagcggttt tgcttttggt catactttta ctggcggctt
    20341 ttttcgctgt tttctggctg gctttctggg tctttttggt ggttgccgtg cttttctttt
    20401 tatctgcttt tttggtgata tgagtggtag cagaactctc cctggcttgc tttgaagcat
    20461 gggccatagg cgttaaaggc agggtggtaa aaagtaaagc acagagcgtg atcgaaatac
    20521 ggtttatccg cgccactgag cgtccctctg ttaaggcagg tctatcatgc ctgcgtctga
    20581 tttacaaaac ttgatgattc taatgcatag aagaagagaa agttaatagc ttttattgtt
    20641 aaaaaatacg tttcgttggc gagcgcaaaa aaatgcagca gtgaaagcta aaaatggtta
    20701 taaatacgcc agtttgccgc gcatcagaga agaaacgaat atttgaaatg caacaaatag
    20761 agcaaaggga taaaatagca gaagcgtgat gaaaatgtta ttttccgatg ccgcactgac
    20821 ccgctacaat gtcagattac tgccgtaaag aagtttgagg aagcaagaca atgagcacca
    20881 ctatcgaaaa aatccaacgc cagattgctg aaaacccgat cctgctgtac atgaaaggtt
    20941 caccgaaact gccgagctgc ggtttctctg cccaggcagt ccaggcgctt gccgcatgtg
    21001 gcgaacgttt tgcctatgtt gatattctgc agaatccgga cattcgtgcg gaactgccga
    21061 aatatgctaa ctggccgacc ttcccgcaac tgtgggttga cggcgagctg gtcggcggtt
    21121 gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca gcagctgatc aaagaaactg
    21181 ccgctaaata caagtctgaa gagccggacg cggaataatc cgtcctgcaa tactgacgga
    21241 tgcgaacggg catccgtcag tcctttatcc ggtgcgccgt aaaacctcgt ccttcagggc
    21301 ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca atgtactggc
    21361 ggatgacgga tattggcgca ccttcgcagc tactggcaaa atagccagga ctccacaaaa
    21421 cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt aacctattcg
    21481 atatgccttt gaggctgttt accagactgg atatcgccag tttgggagga tagttaatta
    21541 acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagctgca aaatcagcac
    21601 atacatttga aaagtaagtg cgtagttttt ctgtcgtgtc atggtcaaaa atctggcgtc
    21661 ggtatttggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc cttccacgcc
    21721 ggatatcggt ttcttttttc acagaccaaa gtatcatttg acctgtgaaa cgattacagg
    21781 catttaaatt ccagttaaga cccggtggtc aacaggagcg tgaaatgagg cgcttcgcag
    21841 gcgcatgtcg tttcgttttc aatcgtgctc tggcgcttca gaatgagaat catgaggccg
    21901 gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaacgcca
    21961 ctgaaacgca atggcttaaa gattcgccct cacagccatt gcaacagtca ctgaaagacc
    22021 ttgagcgggc ttacaaaaac ttcttccaga agcgggctgc ttttccccga ttcaaaaagc
    22081 ggggacagaa tgctgcattc cgctacccgc aggacgttaa gctcgatcag gaaaacagcc
    22141 gtatttttct gccgaaactt ggctggatgc gctaccggaa tagccgtcag gtcacgggag
    22201 ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg gtacatcagt attcagacag
    22261 aaagtgaagt atcaactccg gttcaccctt cagcatcaat gatcgggctg gatgctggcg
    22321 tggctaaact cgccacgctg tcagatggca cagtctttga gcctgtaaac agttttcaaa
    22381 aaaaccagaa gacgctggcg agacttcagc gacagttaag ccggaaggtc aaattcagca
    22441 acaactggca gaagcagaaa cgcaaaatac agcgactgca ttcctgtatc gcaaatatcc
    22501 gcagggacta ccttcacaaa gtcacaacga ccgtcagc
//
LOCUS       DAJVQR010000063        22281 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVQR010000063 DAJVQR010000000
VERSION     DAJVQR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..1028)
                     /locus_tag="OQM52_002581"
     CDS             complement(<1..1028)
                     /locus_tag="OQM52_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564384.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="OQM52_002582"
     CDS             complement(1130..2110)
                     /locus_tag="OQM52_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1564385.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="OQM52_002583"
     CDS             complement(2118..2828)
                     /locus_tag="OQM52_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCS1564386.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OQM52_002584"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OQM52_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1564387.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="OQM52_002585"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="OQM52_002585"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1564388.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="OQM52_002586"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="OQM52_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1564389.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="OQM52_002587"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="OQM52_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1564390.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="OQM52_002588"
     CDS             9405..9515
                     /locus_tag="OQM52_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1564391.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OQM52_002589"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OQM52_002589"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1564392.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OQM52_002590"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OQM52_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1564393.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="OQM52_002591"
     CDS             12397..13029
                     /locus_tag="OQM52_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1564394.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OQM52_002592"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OQM52_002592"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1564395.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="OQM52_002593"
     CDS             16561..17493
                     /locus_tag="OQM52_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1564396.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="OQM52_002594"
     CDS             complement(17534..18964)
                     /locus_tag="OQM52_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1564397.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OQM52_002595"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OQM52_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1564398.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OQM52_002596"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OQM52_002596"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1564399.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="OQM52_002597"
     CDS             complement(20825..21094)
                     /locus_tag="OQM52_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1564400.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="OQM52_002598"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="OQM52_002598"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1564401.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="OQM52_002599"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="OQM52_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1564402.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5583 c         5333 g         5850 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcactgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacat ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcactgg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacactc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgaggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgccca cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DAJVQR010000064        22026 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVQR010000064 DAJVQR010000000
VERSION     DAJVQR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..2299
                     /locus_tag="OQM52_002600"
     CDS             <1..2299
                     /locus_tag="OQM52_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCS1564403.1"
                     /translation="ESLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLR
                     LEISVPQIAIKSNARGYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNL
                     NSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFD
                     SVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEI
                     SDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQ
                     QESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTL
                     ADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGD
                     DEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGY
                     NTQIKDLSLGVSWNYSKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTS
                     NTSIDNEGHITQNLGLTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARV
                     GYNYSDNGSQQQLNYALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLK
                     TDWRGYTVVPYATSYRENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGA
                     RVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQ
                     ICHISYQLTEQQINSAITRMDAICR"
     gene            2290..3273
                     /locus_tag="OQM52_002601"
     CDS             2290..3273
                     /locus_tag="OQM52_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1564404.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3371..3911
                     /locus_tag="OQM52_002602"
                     /pseudo
     CDS             3371..3911
                     /locus_tag="OQM52_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3919..4434
                     /locus_tag="OQM52_002603"
     CDS             3919..4434
                     /locus_tag="OQM52_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1564405.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4400..5137
                     /locus_tag="OQM52_002604"
     CDS             4400..5137
                     /locus_tag="OQM52_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1564406.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5248..6258
                     /gene="pyrD"
                     /locus_tag="OQM52_002605"
     CDS             5248..6258
                     /gene="pyrD"
                     /locus_tag="OQM52_002605"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1564407.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6432..6974
                     /gene="zapC"
                     /locus_tag="OQM52_002606"
     CDS             6432..6974
                     /gene="zapC"
                     /locus_tag="OQM52_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1564408.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(6971..8080)
                     /gene="ycbX"
                     /locus_tag="OQM52_002607"
     CDS             complement(6971..8080)
                     /gene="ycbX"
                     /locus_tag="OQM52_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1564409.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8324..10432
                     /gene="rlmKL"
                     /locus_tag="OQM52_002608"
     CDS             8324..10432
                     /gene="rlmKL"
                     /locus_tag="OQM52_002608"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1564410.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10444..12351
                     /locus_tag="OQM52_002609"
     CDS             10444..12351
                     /locus_tag="OQM52_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1564411.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12481..13734
                     /gene="pqiA"
                     /locus_tag="OQM52_002610"
     CDS             12481..13734
                     /gene="pqiA"
                     /locus_tag="OQM52_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1564412.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13739..15379
                     /gene="pqiB"
                     /locus_tag="OQM52_002611"
     CDS             13739..15379
                     /gene="pqiB"
                     /locus_tag="OQM52_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1564413.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15376..15939
                     /gene="pqiC"
                     /locus_tag="OQM52_002612"
     CDS             15376..15939
                     /gene="pqiC"
                     /locus_tag="OQM52_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1564414.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16195..16362
                     /gene="rmf"
                     /locus_tag="OQM52_002613"
     CDS             16195..16362
                     /gene="rmf"
                     /locus_tag="OQM52_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1564415.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16432..16950)
                     /gene="fabA"
                     /locus_tag="OQM52_002614"
     CDS             complement(16432..16950)
                     /gene="fabA"
                     /locus_tag="OQM52_002614"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1564416.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17019..18779)
                     /locus_tag="OQM52_002615"
     CDS             complement(17019..18779)
                     /locus_tag="OQM52_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1564417.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            18964..19416
                     /gene="matP"
                     /locus_tag="OQM52_002616"
     CDS             18964..19416
                     /gene="matP"
                     /locus_tag="OQM52_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1564418.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19492..20538)
                     /gene="ompA"
                     /locus_tag="OQM52_002617"
     CDS             complement(19492..20538)
                     /gene="ompA"
                     /locus_tag="OQM52_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1564419.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(20894..21403)
                     /gene="sulA"
                     /locus_tag="OQM52_002618"
     CDS             complement(20894..21403)
                     /gene="sulA"
                     /locus_tag="OQM52_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1564420.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21622..>22026
                     /gene="sxy"
                     /locus_tag="OQM52_002619"
     CDS             21622..>22026
                     /gene="sxy"
                     /locus_tag="OQM52_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1564421.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGE"
BASE COUNT         5620 a         5177 c         5771 g         5458 t
ORIGIN      
        1 tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta aacaactgga
       61 caaacaagtg tgtgtgccat tggcggagat tattccggat gccagcgtaa cgtttaatgt
      121 gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta atgcccgtgg
      181 ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg gatattcgtt
      241 tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca gctattttct
      301 gaatttaaac agtggcgtta atttaggccc atggagatta cgcaacaatt caacatggag
      361 tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc agcgggcggt
      421 tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg attttttcga
      481 tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc cagacagcct
      541 gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga taacgattaa
      601 gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg aaattagtga
      661 tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag cggacggcag
      721 cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc aggggcgaat
      781 caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc aggagagcaa
      841 atttgcccag accacgttgc agtggggcgg accgtgggga acgacatggt atggtggtgg
      901 acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc ttggcgattt
      961 cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc aaagcgaaca
     1021 taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg gcactaattt
     1081 tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg acaccatgta
     1141 taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc cgatgtggtc
     1201 gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata tctcccagca
     1261 attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact ggcataccga
     1321 tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc tctcgctggg
     1381 ggtttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg tgtttgcact
     1441 aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct ataccaggaa
     1501 aaaaaattac gcctggatga cctctaacac cagtatcgat aacgaagggc acattacaca
     1561 aaacctgggt ttaacggaga cactactcga tgacggtaac ctgagctaca gcgtgcaaca
     1621 gggatataac agcgagggga aaacggctaa tggtagcgcc agcatggact acaaaggggc
     1681 gtttgcagat gcccgagtgg gctacaacta cagcgataac ggcagtcaac aacaactgaa
     1741 ctacgctctt tcaggcagtt tagttgccca ttcacagggc attaccctgg ggcaatcgct
     1801 gggggaaact aacgtgctga ttgcagcacc aggcgcagag aatactcgtg tggcgaacag
     1861 caccgggctg aaaactgact ggcgcggata taccgttgtc ccctatgcca cttcttatcg
     1921 ggaaaatcga atcgcacttg atgcggcatc gttaaaacgt aacgtggatc ttgaaaatgc
     1981 agtagtcaac gtggttccca ccaaaggggc gttggttctg gcggagttca atgcccatgc
     2041 gggagcaagg gtattaatga aaacatcaaa gcagggtata ccgctgcgtt ttggcgcgat
     2101 agcgacgctg gacggcatac agactaatag cggcattatt gatgatgatg gctcgctcta
     2161 tatgtccggt ttgcccgctc agggagcgat aactgtacgc tggggcgaag ctcccgatca
     2221 aatttgtcat atcagttacc agcttaccga acaacaaatt aactctgcga ttacgcgaat
     2281 ggatgccata tgcagataat ctttggagaa aaatgcgtgt tattactacg actatttttt
     2341 gccgccgtct taatgctatg gtgcgctcaa actgctgctt atatcgggca gtgtcatacc
     2401 actcagggga atccgtatat tggcgtcaat tttggcgtta aaactctgga tgaagaagaa
     2461 aatacggctg gagtagttaa agacaaattt tatcagtgga acgaatcgaa tgattattat
     2521 gtttcctgtg attgcgataa agacaatgtc agaagtggcc gatgggcatt cgccgcggat
     2581 tcaccgttag tctatttagg cgacaactgg tacaaaatta atgactatct tgccgccaaa
     2641 gttttattgc aggttaaagg cagttctcct actgcggttc ctttcgaaaa cgtgggcaca
     2701 ggggcagata cacgatggca tatttgcgat cccggcggtc aacgtttagg tggccagggg
     2761 gctagcggta atagcggtag cttttccctg aaaatattgc agccgttcgt tggttcggtc
     2821 gtcattcctc ctatggcgct ggcgcgatta tttgaatgct acaacatacc cgcaggtgat
     2881 tcctgcacga ctactggtac accggtttta gtgtattacc tgtctggtac gatcaattca
     2941 cttggctcat gttccgtcaa tgccggagaa acaatcgagg ttgatctggg cgacgtattt
     3001 gcggctaact ttcgtgttgt agggcataag cctcttgggg ccagaacggc agaacttgca
     3061 attccggtca ggtgtaacac gggaaacgca gggttagtta acgtcaatct gagtctgacg
     3121 gcaacctcag accccagcta tccccaggcg attaagacgt cacgtcctgg cgtgggcgtg
     3181 gtggtgaccg atagccagca caacattatt tcccctgctg gtgggacact accgctctct
     3241 attcctgatg atgcagacag tatccgcgaa tgaatgtcta tccagtcagc acgacaagcg
     3301 taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat tttgattaac
     3361 gcgtgaacgt atgaagaaaa aaacgatata tcagtgcgtt attttgttct ttagccttct
     3421 taacatccat gtcgggatgg ctgggcctga acaagtcagt atgcatattt atgggaatgt
     3481 ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc atattggtga
     3541 ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg atttgaatat
     3601 tgatatcacc ggttgtgccg ctggtattac tggcgcggac gtccttttta gcggcgaggc
     3661 tgacaccctt gcgccgacac tgctcaaact aactgacaca ggcggaagcg gtggtatggc
     3721 aacggggatt gccgtgcatt cttgatgcgc aaagtcagca agaaatcccg ctcaatcagg
     3781 tccagcctct tacgccctta aaagccgggg ataacacact caaatatcaa cttcgttata
     3841 agtccacaaa ggcgggagca acgggcggta atgcgacggc ggttctctat tttgatctgg
     3901 tttaccagtg aaggagtgat gttgaaacgg ataatctgga ttctgttctt actgggatta
     3961 acggggggct gtgagctatt tgcccatgat ggcacggtca acattagcgg atcgtttcgc
     4021 cgtaatacat gcgtgctggc acaggatagc aagcaaatca atgtgcagtt aggcgatgtc
     4081 tcgctgacac gtttttttca tgggaattat ggcccggaga aatctttcat cattaatttg
     4141 caggattgcg gaacagatgt cagcacggtt gatgtcacct tttcaggaac ccccgacgga
     4201 gtgcagagcg aaatgctgtc cattgaaagt ggtacggatg cagccagtgg gctggcaatt
     4261 gcgattctgg atgatgcgaa gatattgatt ccgcttaacc aggcgagtaa agactacagc
     4321 ctacatagcg gtaaggtgcc attaactttt tatgcgcaat tgcgacctgt caatagcgat
     4381 gtgcagtcgg gtaaagtgaa tgccagcgcg acatttgtac ttcattatga ctaatacgtg
     4441 gaacagatta gcgctcttga ttttcgccgt tttatcgctg ctggtggcgg gtgaattgca
     4501 ggcaggagtg gtggtcggcg gaacgcgatt tatctttccg gcagacagag aatcgatatc
     4561 tattttactg actaatacca gtcaggaatc ttggcttatt aacagcaaaa tcaatcgccc
     4621 aacgcgttgg gccggggggg aagcgtcgac agtgccagca ctattactgg ccgctccgcc
     4681 acttattctc ctgaagcccg gtactacagg cacgttgcgc ttgctgagaa cggaaagcga
     4741 catcttgcct gtggatcgcg aaacgctatt tgagttaagc attgccagcg tgccatccgg
     4801 caaagttgaa aatcagagcg taaaagtggc gatgcgctcg gtatttaaac tgttctggcg
     4861 acccgaaggt ttgccgggcg acccgctgga agcttatcaa caattacgct ggacacggaa
     4921 ttcgcagggt gtacaactca ctaacccaac gccttactac attaacctga ttcaggtgag
     4981 cgtaaatggt aaagcgttaa gtaatgcggg agtcgtgccg cctaaaagcc agcgtcagac
     5041 aaactggtgt caggtaatag caccgtgtca tgtcgcctgg cggacgataa atgattacgg
     5101 cgggttgagt gcaaagaagg agcaaaatct gccctgaaac aggttctgaa aacgtttgcg
     5161 ttttttttgc cgcaggtcaa ttcccttttg gtccgaactc gcacataata cgcccccggt
     5221 ttgcacaccg ggaatccagg agagttcatg tactacccct tcgttcgtaa agcccttttc
     5281 cagctcgatc cagagcgcgc tcatgagttt acttttcagc aattacgccg cattaccgga
     5341 acaccgtttg aagcactggt gcggcagaaa gtgcctgcga aacctgttaa ctgcatgggc
     5401 ctgacgttta aaaatccgct tggtctggca gccggtcttg ataaagacgg ggagtgcatt
     5461 gatgcgttag gcgcgatggg atttggatcg atcgagatcg gtaccgtcac gccacgtcca
     5521 cagcctggta atgacaagcc gcgtctcttt cgtctggtag atgccgaagg tttgatcaac
     5581 cgtatgggct ttaataatct tggcgttgat aacctcgtag agaacgtaaa aaaggcccat
     5641 tatgacggcg tcttgggtat taatatcggc aaaaataaag atacgccagt ggagcagggc
     5701 aaagatgact atctgatttg tatggaaaaa atctatgcct atgcgggata tatcgccatc
     5761 aatatttcat cgccgaatac cccaggatta cgcacgctgc aatatggtga agcgctggat
     5821 gatctcttaa ccgcgattaa aaataagcaa aatgatttgc aagcgatgca ccataaatat
     5881 gtgccgatcg cagtgaagat cgcgccggat ctttctgaag acgaattgat ccaggttgcc
     5941 gatagtttag ttcgccataa tattgatggc gttattgcaa ccaataccac tctcgatcgt
     6001 tctctggttc agggaatgaa aaattgcgat caaaccggtg gcttaagtgg tcgtccgctt
     6061 cagttaaaaa gcacagaaat tattcgccgc ttgtcactag aattaaacgg tcgcttaccg
     6121 atcatcggtg ttggcggcat cgactcggtt atcgctgcgc gtgaaaagat tgctgcgggt
     6181 gcctcactgg tgcaaattta ttctggtttt atttttaaag gtccgccgct gattaaagaa
     6241 atcgttaccc atatctaatt atttcttcga attcctcttt ataagataat cagggcttta
     6301 ttttcagacc tggttgtttt atattcatcg ttgttgctta tttagacatt ttgtactttt
     6361 attgttgagg ttattaagcg aagcgacaat ggattgtgtt gctgcggttt tatagggtag
     6421 gggagaggca gatgcgaatt aaaccagacg ataactggcg ttggtattac gatgaagagc
     6481 acgatcgaat gatgctcgat ttagccaatg gtatgctatt tcgctcacgt tttgcgcgca
     6541 aaatgttgac tccagatgcc ttttctcccg caggcttttg tgttgatgat gccgcgctct
     6601 atttctcttt tgaagaaaag tgccgcgatt ttaatttatc taaagaccaa aaagccgaac
     6661 tggtgctgaa tgcactggta gcaattcgtt atctcaaacc gcaaatgccg aaaagctggc
     6721 attttgtctc ccatggtgaa atgtgggtgc ccatgccggg cgatgcggcc tgcgtttggt
     6781 tgagcgatac tcacgagcag gtcaatttgc tggtcgttga atctggcgaa aatgccgcgc
     6841 tatgcctgct ggcacaaccc tgcgttgtta ttgcgggtcg cgcgatgcag ttgggcgacg
     6901 ccattaaaat catgaacgac aggctgaaac cgcaggtgaa tgttgacagc ttcagcctcg
     6961 aacaggcagt ctaacgcgtc agcttaaggg cagtcttcgg tacacagcta cagcaaagaa
     7021 tggtgccatc atcgcccatt gctgattttt tcagcggtgt gacttcgcct tctaaaagct
     7081 gaacacggca acttccacaa atgcccgcgc ggcaagaata agggatacga attccctgat
     7141 tttctaattg ctccagcaac acttgttggt tatttccacg aaatgcctgt ccctgccagt
     7201 caatatctac atttgcgtcc ggttgttgcg tgatgttggc ggtatcatca gcggcagctg
     7261 cgccgtaaat ttttgccgga gccgttgcca gaatttccac ctcatcgcca acgcgaatca
     7321 cgccgctatt acgggcaatt aaattctgac caaaatcgac atcgccgtta tcctgggcag
     7381 tgcggaaaga ttgcaatgtt tttaatggtt cgcctgccgg atgtttttgc cctttttctg
     7441 ggctgacggt ggtgaaaata cagcggctac aaggtttaac cacatcaaac accacatcac
     7501 caatgcgaat cactttccag ctatcttctt cccaggctga cgcgccggaa accaccagat
     7561 tggggcggaa ttgctccatt tttacactgg ccggacaacg ttgttggaga tcacgtaacg
     7621 aggcttcgtt agcaagaagg taaggatagc catcagcaaa tgacagaggt acagtgttgt
     7681 ggcgtttcac gcgccgggtc atttgtggcc ccacccagcg taattgcact tcgcgggaga
     7741 aaaatccact tagccatttg ttgatcgcgt ctggcgcaat tcgcgcggta aaatgtgtgc
     7801 cccaaacttc ggttggtgcg tcttgtgtgg cgaaatcagc aaaacgaaca tatgcgctac
     7861 tgccatctgg tgcggtgaga tgcaagccat catgcacagg tgaaggagta aaccgtacca
     7921 tctggggaaa ctggcgagcg gtaataaacg taccgtcagg ttccgtgatc ataaagatgc
     7981 gatcgaaggc cagaccactg acatcagcca gagcatgtgt aagaccaatg ccgcgcatcg
     8041 atttaacagg atgaataaaa agccgggtta atgtcgccac ggtgcggtcc tcaaatgaaa
     8101 ataagccctc aactttatga catgacgcgc ttattagcta taatgcgcaa caattttctt
     8161 agcgcctgtc ccgttaggct attttatttg tcagtttggc cctgggttgt gctcgaaatc
     8221 ctcacgtact tgtgtacgct ccggtttctc cgcgctctcc atatccagac tgactgcaac
     8281 aattacgcct actgcggtag gctcttagag taaaagtgac gatatgaatt ctctgtttgc
     8341 cagtacggcc cgtgggctgg aagagctgtt aaaaactgaa ctggaaaatc tgggggccgt
     8401 tgaatgccag gtggttcagg gtggggtcca tttcaagggc gacacacggc ttgtttacca
     8461 gagcctgatg tggagccgcc tggcctcgcg tattatgttg ccgctgggcg agtgtaaggt
     8521 ttacagcgat ttagacctct atctcggtgt tcaggcgatc aactggacag agatgtttaa
     8581 tcctggcgcg accttcgctg tccacttcag tggtttgaat gacaccatcc gcaacagtca
     8641 gtacggcgcg atgaaagtga aagacgcgat cgtcgatgct ttcacacgga aaaatctgcc
     8701 gcgtccaaat gttgatcgcg atgcgccgga tatccgcgtt aacgtctggc tgcataaaga
     8761 aaccgccagt atcgctcttg atctgagtgg tgatggttta catctgcgtg gctatcgcga
     8821 tcgtgctggt attgcgccga tcaaagaaac cctggcagcc gctattgtga tgcgatccgg
     8881 ctggcagtca ggaacaccgc tgctcgatcc gatgtgtggt tccggtacgt tgctgattga
     8941 agcggcgatg ctggcgaccg atcgcgcacc aggcttgcac cgtgggcgtt ggggctttag
     9001 cggctgggcg cagcatgatg aagctatctg gcaggaagtg aaagcggaag cgcaaactcg
     9061 cgcccgtaaa ggcctagctg agtatagctc tcatttttac ggttcggaca gcgacgcacg
     9121 ggtgattcaa cgtgcgcgca ctaacgcccg tcttgcgggg attggtgaac tgatcacctt
     9181 tgaggtgaaa gatgtcgcgc aactgaccaa tccgctgccg aaagggccgt acggtacagt
     9241 gttgagcaac ccgccatacg gtgaacgtct ggacagcgaa ccggcgctga ttgcgctgca
     9301 tagtctgctg ggccgaatca tgaaaaacca gttcggtggc tggaatctct ctttgtttag
     9361 tgcctcgccg gatctgctaa gctgcctgca gctgcgtgca gacaaacagt acaaggcgaa
     9421 aaacggcccg ctggactgcg tacagaaaaa ttaccacgtt gccgaaagca caccagacag
     9481 taaaccggca atggtagcgg aagattacgc caaccgcctg cgtaagaacc tcaaaaaatt
     9541 cgagaagtgg gcacgccagg aagggattga atgttaccgc ctgtatgacg ccgatctgcc
     9601 ggaatataac gttgccgttg accgttatgc cgactgggtg gtggtgcagg agtatgcgcc
     9661 gccaaaaact attgatgcgc acaaagcgcg tcagcgtctg ttcgatatta tcgctgcaac
     9721 catttcggta ctggggattg cgccaaacaa actggtgctg aaaacccgtg aacgccataa
     9781 gggcaaaaac cagtaccaga aactgggcga gaagggcgag tttcttgaag ttaccgaata
     9841 taacgctcac ttgtgggtga acctgacgga ttatctcgat actggtctgt tcctcgatca
     9901 ccgtatcgcc cgccgtatgc tcggtcagat gagcaaaggc aaagatttcc tcaacctgtt
     9961 ttcttatacc ggcagcgcca ccgtgcatgc gggattaggc ggtgcacgca gcaccaccac
    10021 cgtggatatg tcgcgtactt atctggaatg ggcagaacgc aacctgcgtc tgaatggcct
    10081 gactaggcgt gcgcatcgcc tgattcaggc cgattgcctg gcgtggctgc gtgaggcaaa
    10141 tgaacagttc gatctgatct ttatcgatcc gccaaccttc tctaactcaa aacgaatgga
    10201 agatgcgttt gatgttcagc gcgatcatct ggcgctgatg aaagatttga aacgtctgct
    10261 gcgtgcaggt gggacgatca tgttctcgaa caacaaacgt ggcttccgta tggatctcga
    10321 cggcctggca aaactgggac tgaaagcaca agaaattacg caaaaaacgc tctcccagga
    10381 tttcgcccgt aaccgccaga tccacaactg ctggctgatt accgcagcct gaaaggaata
    10441 gtaatgtcat taatcagtat gcatggcgca tggctgtcgt tcagcgacgc gccgcttctc
    10501 gataacgcag aactgcatat cgaagataac gaacgtgttt gtctggtggg ccgcaacggc
    10561 gcaggaaaat cgacgttaat gaaaatcctc aaccgtgaac aagggctgga tgacggtcgc
    10621 attatttatg agcaagattt gattgtagct cgtctgcaac aggatccgcc gcgtaacgtt
    10681 gagggtagcg tttatgattt cgttgccgaa ggcattgaag aacaagcgga atatctgaaa
    10741 cgctatcacg atatttcgcg cctggtgatg aacgacccga gcgagaaaaa tctcaacgaa
    10801 ctggcgaagg ttcaggaaca gctggatcac cacaacctgt ggcagctgga aaaccgcatc
    10861 aacgaagtgc tggcgcaact ggggttagat cctaacgttg cgctgtcgtc gctttccggc
    10921 ggctggttgc gtaaagcggc attaggacgc gcgctggtga gcaatccgcg cgtattgctg
    10981 ctggacgaac cgactaacca tctggatatc gaaaccatcg actggctgga ggggttcctt
    11041 aaaaccttca atgggacgat tattttcatc tcccacgacc gttcgtttat ccgcaatatg
    11101 gcgacgcgca ttgttgatct cgatcgcggc aagctggtga cctatccagg gaattacgac
    11161 cagtacctgc aggaaaaaga agaagccctg cgcgtggaag aactacaaaa tgccgagttc
    11221 gatctcaaac tggcgcagga agaagtgtgg attcgccagg ggatcaaagc acgccgtacc
    11281 cgtaatgaag gccgcgtacg cgccctgaaa gcgatgcgtc gcgaacgtgg tgaacgtcgc
    11341 gaagtgatgg gtaccgcaaa gatgcaggtg gaagaggcca gccgctccgg taagatcgtt
    11401 ttcgaaatgg aagacgtttg ctaccaggtt aacggtaagc aactggtgaa agatttttct
    11461 gcccaggttc tacgtggcga caaaattgcc ctgattggtc cgaatgggtg cggcaaaacc
    11521 acgctgctga aactgatgct cggtcagctt caagcggaca gcgggcgtat tcacgttggc
    11581 accaaactgg aagtggctta tttcgatcag caccgcgcgg aactggatcc cgataaaacg
    11641 gtgatggata accttgccga aggtaagcaa gaggtgatgg ttaatggcaa gccacgccac
    11701 gtattgggct atttgcagga ctttctgttc catccgaaac gggcgatgac gccagtacgt
    11761 gcgttttctg gcggtgagcg gaaccgcttg ctgctggcgc gtttgttcct caaaccaagc
    11821 aacttattga ttcttgacga accgaccaac gatcttgatg tcgaaacgct ggaactgctg
    11881 gaagaactga tcgacagcta tcagggcacg gtattgctgg ttagccacga tcgtcagttt
    11941 gtcgataaca ccgttacaga atgctggatc ttcgaaggcg gcggtaaaat tggtcgttat
    12001 gttggcggtt atcatgatgc ccgtggtcag caagaacaat atgtggcact caaacagcct
    12061 gcggtgaaaa aaagcgaaga agccgccgcg ccaaaagcag aaactgtaaa acgcagcagt
    12121 agcaaactaa gctataaatt gcagcgcgaa ctggagcagc taccgcaatt gctcgaagat
    12181 ctggaggcga agctggaagc cctacagacg caagtggcgg atgcttcctt cttcagtcag
    12241 ccgcatgagc agacgcaaaa agtgcttgct gatatggctg ctgcagagca ggagctggag
    12301 caagcctttg aacgctggga gtatcttgaa gcgttaaaaa atggtggctg atcgcaaaat
    12361 agtcgattta aacagagaca gctgacaacg gtaaaattgc ctgatgaact gggtttatca
    12421 ggcatgaaaa ccaacacttt gttagtcatc tgtaaaatag cgcatcatta aggagtacca
    12481 atgtgcgaac atcatcatgc cgcgaagcac atcctgtgct cgcagtgtga catgctggtg
    12541 gcgttaccgc gccttgagca tggtcagaaa gcggcatgtc cccggtgtgg cacaacgtta
    12601 accgtggcgt gggatgcccc tcggcagcgt cccaccgcct atgcgttggc tgcactgttc
    12661 atgctgttgc tgtccaactt gtttcctttt gtgaatatga acgttgcagg agttaccagt
    12721 gaaattacat tactggaaat tcccggcgtg cttttttctg aggactacgc cagcctcggc
    12781 acctttttcc tgttgtttgt gcaactggtt cccgcgtttt gtctgataac cattctgtta
    12841 ctggtgaatc gcgcggaatt accggtccgt ttaaaagagc aactggcacg ggtgcttttt
    12901 caactcaaaa cctggggaat ggcggagatt ttcctcgcgg gtgtgctggt cagtttcgtt
    12961 aaactgatgg cttacggcag cattggggta ggcagcagct ttctcccctg gtgtttattt
    13021 tgtgtcctgc aactgcgcgc ttttcagtgc gttgatcgtc gctggttatg ggacgacatc
    13081 gccccgatgc cagaactgcg ccagccgcta aaaccaggcg tcacggggat acgtcagggg
    13141 ctgcgttcgt gctcctgttg tacggcaatc cttcctgctg atgaacccgt gtgcccgcgt
    13201 tgtagtacca aagggtacgt tcggcgtaga aacagcctgc agtggacact cgcgctgctt
    13261 gtaacgtcca tcatgctgta tcttccggct aatattttgc ccatcatggt gacggattta
    13321 ttaggctcga agatgccgtc gacgattctc gctggggtca ttctgttatg gagcgaaggc
    13381 tcttatcccg tcgctgcggt gatctttctg gccagtatta tggtgccaac gttaaagatg
    13441 atcgccatcg cgtggctgtg ttgggatgcc aaagggcatg gcaagcgcga cagtgaaaga
    13501 atgcatttga tttatgaagt tgttgagttt gtaggccgct ggtcgatgat tgacgttttc
    13561 gttatcgcgg tgctctcggc gctggtgcgt atgggaggtt taatgagtat ttatccggca
    13621 atgggtgcat taatgtttgc tttagtcgtc ataatgacaa tgttttctgc tatgacgttt
    13681 gacccgcgtt tgtcgtggga tcgtcaacct gaatcagagc atgaggagtc ctgacagtat
    13741 ggaatctaat aatggggaag ccaaaatcca gaaagtgaag aactggtctc ccgtgtggat
    13801 atttcctatc gtcacggcgc tcattggggc ctgggttctt ttttatcatt acagccatca
    13861 gggaccggaa gtgaccctga tcaccgcgaa tgcggaagga attgaaggtg gcaaaaccac
    13921 cattaaaagc cgtagcgttg acgtcggcgt ggttgaaagc gccacactgg ctgatgattt
    13981 gacgcacgtt gaaatcaaag cgcggctgaa ttccggtatg gaaaaattgc tgcataaaga
    14041 caccgtcttt tgggtggtga aaccgcagat tggtcgcgaa gggattagcg gcctgggaac
    14101 gctgctgtct ggagtttata tcgaactgca gccaggcgcg aaaggcagca aaatggataa
    14161 atacgatttg ctggactcac caccgttggc cccgcctgat gcgaaaggta tccgtgtgat
    14221 tctcgatagc aaaaaagccg ggcagctctc gccaggagat ccggtgctgt tccgtggcta
    14281 tcgggtaggt tcggttgaaa ccagcacctt cgatacgcaa aaacgcaata tcagttatca
    14341 actgttcatc aatgcacctt atgaccgact ggtgaccagc aatgttcgct tctggaaaga
    14401 tagtggcatt gcggttgatc tgacgtcagc ggggatgcgt gtggagatgg gctcattgac
    14461 aacgctgctt agtggcggtg ttagctttga tgtgccggaa ggtctggatt tagggcagcc
    14521 agtggcaccg aaaacagctt tcgttttgta tgatgatcag aagagcattc aggattcgtt
    14581 gtacaccgat cacattgatt atctgatgtt ctttaaagat tcggtacgcg gtctgcaacc
    14641 gggagctccg gtagaattcc ggggtattcg cctgggtacc gtaagcaaag tgccattctt
    14701 tgcgccgaat atgcgtcaga catttaacga tgattaccgt attccggtac tgattcgtat
    14761 cgagccagag cggctgaaaa tgcagcttgg cgaaaatgcg gatgttgttg agcaccttgg
    14821 cgaattgttg aaacgtggtt tacgcggatc gctgaaaacc ggaaacctgg tcactggtgc
    14881 actgtatgtt gatctcgatt tctatccaaa tacgcctgca ataaccggta ttcgtgaatt
    14941 taatggttat cagattatcc cgactgttag cggcggcttg gcgcaaatcc agcaacgact
    15001 gatggaagcg ttggataaga tcaacaaact gccattgaat ccgatgattg aacaggcaac
    15061 cagtacgctt tctgaaagtc agcgcacaat gaaaaacctg caaacgacgc tggatagcat
    15121 gaacaagatc ctcgccagcc agtcgatgca gcagttgccg acggatatgc agtcaacgtt
    15181 gcgtgaattg aatcgcagca tgcagggctt ccagcctggc tccgcagcct acaacaagat
    15241 ggtggcggat atgcagcgcc ttgatcaggt gttgcgagaa ctgcaaccgg tgctgaaaac
    15301 gctcaatgag aagagtaacg cgctggtatt tgaagcgaag gacaaaaaag atccagagcc
    15361 gaagagggcg aaacaatgaa aaagtggcta gtgacgattg cagcactgtg gctggccgga
    15421 tgcagctccg gcgaaattaa taaaaactat taccagttac ctgtggtgca gagcggtaca
    15481 caaagtaccg ctagccaggg caatcgtctg ttatgggtag agcaggtcac tgttcctgac
    15541 tatctggcgg ggaatggtgt ggtttatcaa accagtgatg tgaagtatgt gattgccaac
    15601 aacaacttgt gggccagccc gttggatcaa cagttgcgca acaccctggt tgccaacctg
    15661 agtacgcaac tgcccggctg ggtggttgcc tcccagcctc tgggaagcgc ccaggacacg
    15721 ctcaatgtta ccgtaacgga gtttaacggt cgctatgatg gcaaggtcat tgtcagtggt
    15781 gagtggctgt tgaaccacca gggacaactg atcaaacgtc cgttccgtct ggaaggagtg
    15841 caaactcagg atggttacga tgagatggtt aaagtgctgg ccggtgtctg gagtcaggaa
    15901 gccgcttcta ttgcacaaga gataaagcgt ctaccttaat tataaagatt tgtaaatata
    15961 accgtctccg gtatgttgcc tgaggcggtt tttttgtttc taacgtgcgg aaaaatttgt
    16021 tcctcttcac attttttgta caaccgacat gcccgtgtag ctcacaaata tgacagtggc
    16081 gtgaattttg cgcattgacg gcagttatga ttcgcggtat tgcttaactg tgattgcaca
    16141 tttagtaatc actgttttct tttccaccag aaaccagtat gagggaaacg aggcatgaag
    16201 agacaaaaac gagatcgcct ggaacgggca catcaacgtg gttatcaggc cggcatcgcc
    16261 ggacgctcaa aagaaatgtg tccctatcag acgctgagtc aaaggtcaca atggctggga
    16321 ggctggcgag aagccatggc ggacagggta gtaatggcct gattctgtct ctttaaaaag
    16381 aaacctccgc attgcggagg tttcgccttt tgatactctg tctgatgata atcagaaggc
    16441 agacgtatcc tggaacagac cgactttcag gtcgctggcg gtatagatca gacgacaatc
    16501 aaccagcact tcgccatctg ccagacccat aatcagacga cgattaacaa tgcgtttaaa
    16561 gtgaatacgg taggtcactt ttttcgctgt cggcagtacc tgaccagtga atttcacttc
    16621 gccaacgccc agcgcgcggc ctttaccttc gccgcccagc cagccgaggt agaaccctac
    16681 cagctgccac attgcgtcca ggcccaggca tcccggcata accggatcgc caatgaagtg
    16741 gcatccgaag aaccacagat ccggattgat atccagttct gcttcaacat accctttgtc
    16801 gaagttacca cccgtttcgg tcattttgac cacacggtct atcatcagca tgttcggtgc
    16861 tggcaattgc gggcctttag cgccaaacag ttcaccgtga ccagaggcaa gaaggtcttc
    16921 ttttgtatag gattcgcgtt tatctaccat gttctctgta agccttattt tattgaagca
    16981 cgcaggatag ctaacacgtg tacgctgaac aagtccgatc agttcggaat aaaccagttc
    17041 agccaacgta atggccatgg aaaacggtga cgtccttcct gttgcgatgc ttgcgcgata
    17101 cgttcctgga tggtttgcat cagcgtcgtt tggccttcgc catcccacac cagatttaat
    17161 aataacggca gtgcgtcagt cacatcgtct actgcccaga tggtgaattt gccttcttct
    17221 accgctttca ccagttcact gtgaagactt aaatggcgaa cgttagcagt ggggataatg
    17281 acaccttgtt tccccgttaa ctcacgttgc tggcaaatag caaagaagcc ttcgattttc
    17341 tcatttaaac caccgaccgg ctggacgcgg ccgaactgat cgactgaacc tgtgatagcg
    17401 atactctgat tcactggcac atcagcgagg gcgcttatca gggcgcagag ttcagccatc
    17461 gaggcactat cgccatccac ttcactgtat gactgctcaa atgtcagcga tgctgagaag
    17521 gggatctgtt gctcaagctg tagttccgac atcaggaacg cctgcatgat catcatccct
    17581 ttcgcatgga tattgccgcc aagctccgct ttgcgttcga tgtcggtgaa ttcaccatcg
    17641 ccaatatgca caacgcagct aatgcgagaa ggttcgccaa aagcgcgtgg atgacccgga
    17701 aattcaatga ccgaaagggc gttaatttgc ccgatgcgtt cgccttcggt ttcaatcagg
    17761 atttgctcct gaaggatctc atcctgcata cgttcagcga ggaaaccttc gcgccattca
    17821 cgctgctgca gcattaagtt tagctgctcg ccggagaagg tgtcgccatc acacagggag
    17881 gcgacctctt tacactggcg gaggatccac tgcgggctaa gcggaagtgt ttcttgttca
    17941 ccggtgtagc gtgctgcttc gcggataagt accggccaag catccgctcc cggggcaggt
    18001 aagtgattat gtctggcggt aaatgtcacc cagcgacacc actggcttac tgactccgca
    18061 tcgacaatct gcagagtatc ttcaaattcg ctataaatag cctgctctga aagctctggc
    18121 tccatctcct ggaaatcagc caatgattcg cgttcgccta ccagaatgac tttcagcttc
    18181 aatggcattg aaggcacaga gacggggaga gggcgcgact catcaaacgc aacccagtca
    18241 aaacgctcgc ggttaacgat atttttcagc cgcatccaca gcagaggttg cgccagcagt
    18301 gtacgcaaag agatgatgag aataccgcca tttgcctgat gcaccagacc aggctgcagg
    18361 gtaatgtcgc cattaaactg gcgcaggcag ccaaagagtt gctccgcttc tacccagtcg
    18421 gcagcgacaa cttgcgttaa agtcgcaaaa ttatcatctg cactcactgc gtgacgtaag
    18481 cggatggagt ggccggaaac ctcatagtga tcgcccacca gttggcctgc atcgctttgt
    18541 agcgtacgcg cggcatcggc aataagattc agatactcag actcttccgg ggccttagcc
    18601 agcataaaag aggaggatgc tcgcgtatgc agcaactgct ccagcgcaaa ttgcagtcgc
    18661 ggttgagtat cactgaataa aggatcgttt tcgtcaatca aatgtggctg agcaaatatt
    18721 tcctgatagc tatcggtatc aggaaccagg tcacgccatg caagtttcgt aatggtcaaa
    18781 gttgatgttt tttagtctgt tgtcaaagcc gcgattatac cgtaaccggc actacagcac
    18841 acgtagaaag caccgacaat actcctggct gggcgttaaa gctcacagga tggagattct
    18901 tttcttcact ggcctaaaaa gctgatattc tgtaaagagt tacacggtaa cattgagatc
    18961 gctatgaaat atcaacaact tgaaaatctt gaaagcggtt ggaaatggaa gtatctggtg
    19021 aagaagcatc gcgaagggga gttaattacc cgttacatag aagccagtgc cgcccaggaa
    19081 gccgtcgatg tgttgctctc gctcgaaaat gaacccgtgc tggtaaatgg ctggattgac
    19141 aagcatatga atccggaact ggtcaatcgc atgaagcaga ccattcgggc cagacggaag
    19201 cgccatttta atgcggaaca ccagcatacg cgcaaaaaat cgatcgatct ggaatttatt
    19261 gtctggcaac gtctggctgg tcttgcacag cgtcgcggta aaacgctttc tgaaacgatt
    19321 gttcagctga ttgaagatgc ggaaaacaaa gagaaatacg cgaataaaat gtcttctctt
    19381 aagcaggatc tgcaggcatt gctgggtaag gaataactga cgaaagtcag ttcaatttac
    19441 taaaggcaaa aaaaaccccg cagcagcggg gtttttctac cagacgataa cttaagcctg
    19501 cggctgagtt acaacgtctt tgataccttt aacttcgatc tctacgcgac gatccggagc
    19561 caagcagtcg atcagtgcag cacgctgttt cacgttgtca caggtgttgc cagtaaccgg
    19621 gttggattcg cccataccac gtgcggagat cttgtctgcc gggatacctt tggagatcag
    19681 gtaatcaaca acagactgag cacggcgctc ggacagaccc tggttgtaag cgtcagaacc
    19741 gatgcggtca gtgtaaccca gaacaactac ggaaccgtct ttcggatcta ggttgctcag
    19801 ctggctgtac agctgatcca gagcagcctg accttccggt ttcagggttg ctttgttgaa
    19861 gttgaacaga acgtcagact tcagagtgaa gtgcttggtc tgtacttccg gtgccggagc
    19921 tggagcaact accggagctg cttcgccctg accgaaacgg taggaaacac ccaggctcag
    19981 cagaccgttg tccggacgag taccgatggt gtttgcgtca ccgatgttgt tggtccactg
    20041 gtattccaga cgggtagcga tttcaggagt gatcgcgtac tcaacaccac ctgcgaaaac
    20101 cggagaaacg ccagtgtcgt ggtctttaaa ggatgcgcca ccaggtacgt tagccttggt
    20161 gtctgcacgc cataccatac cacccagacg agtgtagatg tccagatcgt cagtgattgg
    20221 gtaacccagt ttagcggtca gctgaacgcc ctgagctttg tatgcgccgt tgatgttgtc
    20281 gcctttgtac ggcatacgac ctaaccagtc gtaacccatt tcaaaaccaa catacgggtt
    20341 aacctggtaa ccaccaaaag cacctgcacc cagttggttt tcgtgggtcg gaccattgtt
    20401 aggaataaaa ccagtgtcat ggtactggga ccagcccagt ttagcaccag tgtaccaggt
    20461 gttatctttc ggagcggcct gcgctacggt agcgaaacca gccagtgcca ctgcaatcgc
    20521 gatagctgtc tttttcattt tttgcgcctc gttatcatcc aaaatacgcc atgaatatct
    20581 ccaacgagat aacacggtta aatccttcac cgggggatct gctcaatatt aactctaccg
    20641 atatcttcgg cttatgccga gcacccctgg cgatgtaaag tctacaacgt agttgaaaac
    20701 ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc ttgtataagg tatgtttaat
    20761 ctttttgtca gcgacaattt acagaaaaaa aacgcggaaa ccgcttcaga caagccttcg
    20821 caaggaaaat tagtcacgac tgaaagcatt ggctgggcga caaaaaaagt tctaggatta
    20881 atcctaaatt tacttaatga tacaaattag agtgaatttt tagcccggaa agttgtctcg
    20941 ttgtgtgaga ggatgcgctt accggacgca taataaaccc catagcgtta ccttcatttg
    21001 ccgcatcaac aagttcagca tgctcttctt cagtcaaatc atctgtcaac caaccgatca
    21061 ccacactgta attgcccgtg cgtaaagcgc gaaccattga ctccactgtg tggcaagggg
    21121 agagctggct aatctgcatt actttcgtta agggtagccc agatgcctga acccattccc
    21181 gactcagttt ttgttgcggt gttaaccaga gttgccagcg cgattgctga ccgagttgct
    21241 gtaacaatgg caacagtaga agttgcgtca tcatgggctg atcttcgcga tagacaactt
    21301 cactgataag cccggctgta gtgttttccg tagagacacg cgcaatttta cttgctgcgg
    21361 atgagaacga cgaagaacga tgtgcatagc ctgaagtgta cataatcaat ccagcccctg
    21421 tgagttactg tatggatgtg cagtacatcc agtgacaaca aagatcaacc ctattttcgg
    21481 aaagagcctc gcaaattttg tcgttggtga cgggaaaaca taaattaatc ttgcccctta
    21541 agaataagtt gcctattttc gtagttaacg gatccgttaa tgtgaatcat tcttttatgt
    21601 tatgatttta aaaggaattt tatgaaaagc ctctcctata agcggatcta taaatcacaa
    21661 gaatacctgg caacgttggg cacaattgaa taccgatcat tgtttggcag ttacagcctg
    21721 accgttgacg acacggtgtt tgcgatggtt tctgatggtg agttgtatct tcgggcttgt
    21781 gagcaaagtg cacagtactg tgtaaaacat ccgcctgtct ggctgacata taaaaagtgt
    21841 ggccgatccg ttaccctcaa ttactatcgg gttgatgaaa gtctatggcg aaatcaactg
    21901 aagctggtgc gtctgtcgaa atattctctc gatgcagcgc tgaaagagaa aagcacgcgc
    21961 aatacccggg aaagactgaa agatttgccc aatatgtctt ttcatctgga agcgattctc
    22021 ggggag
//
LOCUS       DAJVQR010000065        21610 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVQR010000065 DAJVQR010000000
VERSION     DAJVQR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..85
                     /locus_tag="OQM52_002620"
     CDS             <1..85
                     /locus_tag="OQM52_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1564422.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(143..2212)
                     /gene="glyS"
                     /locus_tag="OQM52_002621"
     CDS             complement(143..2212)
                     /gene="glyS"
                     /locus_tag="OQM52_002621"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1564423.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2222..3133)
                     /gene="glyQ"
                     /locus_tag="OQM52_002622"
     CDS             complement(2222..3133)
                     /gene="glyQ"
                     /locus_tag="OQM52_002622"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1564424.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3228..3527)
                     /locus_tag="OQM52_002623"
     CDS             complement(3228..3527)
                     /locus_tag="OQM52_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1564425.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3702..4697
                     /gene="wecH"
                     /locus_tag="OQM52_002624"
     CDS             3702..4697
                     /gene="wecH"
                     /locus_tag="OQM52_002624"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1564426.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4739..5176)
                     /locus_tag="OQM52_002625"
     CDS             complement(4739..5176)
                     /locus_tag="OQM52_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1564427.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5222..5563)
                     /locus_tag="OQM52_002626"
     CDS             complement(5222..5563)
                     /locus_tag="OQM52_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564428.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5732..7186)
                     /gene="xylB"
                     /locus_tag="OQM52_002627"
     CDS             complement(5732..7186)
                     /gene="xylB"
                     /locus_tag="OQM52_002627"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1564429.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7258..8580)
                     /gene="xylA"
                     /locus_tag="OQM52_002628"
                     /pseudo
     CDS             complement(7258..8580)
                     /gene="xylA"
                     /locus_tag="OQM52_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8946..9938
                     /gene="xylF"
                     /locus_tag="OQM52_002629"
     CDS             8946..9938
                     /gene="xylF"
                     /locus_tag="OQM52_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1564430.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            10016..11557
                     /gene="xylG"
                     /locus_tag="OQM52_002630"
     CDS             10016..11557
                     /gene="xylG"
                     /locus_tag="OQM52_002630"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1564431.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11535..12716
                     /gene="xylH"
                     /locus_tag="OQM52_002631"
     CDS             11535..12716
                     /gene="xylH"
                     /locus_tag="OQM52_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCS1564432.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12794..13324
                     /locus_tag="OQM52_002632"
     CDS             12794..13324
                     /locus_tag="OQM52_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1564433.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13436..13972
                     /locus_tag="OQM52_002633"
     CDS             13436..13972
                     /locus_tag="OQM52_002633"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1564434.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(14080..14904)
                     /locus_tag="OQM52_002634"
     CDS             complement(14080..14904)
                     /locus_tag="OQM52_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1564435.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15224..17254
                     /gene="malS"
                     /locus_tag="OQM52_002635"
     CDS             15224..17254
                     /gene="malS"
                     /locus_tag="OQM52_002635"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1564436.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17432..18685
                     /gene="avtA"
                     /locus_tag="OQM52_002636"
     CDS             17432..18685
                     /gene="avtA"
                     /locus_tag="OQM52_002636"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1564437.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18836..19309)
                     /locus_tag="OQM52_002637"
     CDS             complement(18836..19309)
                     /locus_tag="OQM52_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1564438.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19411..20259)
                     /gene="yiaJ"
                     /locus_tag="OQM52_002638"
     CDS             complement(19411..20259)
                     /gene="yiaJ"
                     /locus_tag="OQM52_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1564439.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20459..21457
                     /gene="yiaK"
                     /locus_tag="OQM52_002639"
     CDS             20459..21457
                     /gene="yiaK"
                     /locus_tag="OQM52_002639"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1564440.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21469..>21610
                     /locus_tag="OQM52_002640"
     CDS             21469..>21610
                     /locus_tag="OQM52_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1564441.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5439 a         5511 c         5301 g         5359 t
ORIGIN      
        1 acggaagtca ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta
       61 tctgaacata aaacactatc agtaagttgg agtcattacc aaaaaagcct gccagatggc
      121 aggcttttta ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca
      181 gttcgcgcag tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca
      241 tgaccatcac tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca
      301 gcgcatcctg gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa
      361 cctgcatcgc cagtttaatt tcttccggct ctttcagggt agaggcattc acgcgatcgc
      421 tcagcacttc gtcagatttc gccaggatgt tagatacacg tttgttcgcc gccgccagtg
      481 ctgcagctgc ttccagggta cggaagtgcg ataccgcttt catgcgggca tcgaaatcag
      541 ccggacgagt cggacgacgc gccagtaccg cctggatggt gtcaacggta taaccttcgt
      601 cctgatacca ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg
      661 cattagtcag cttatcgcca tacagacgca ccgcttcttc ggtcagcgtt tgcagatcaa
      721 ggttgaggtt cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa
      781 acgggtcttt gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca
      841 tcttatcagc aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa
      901 agcgcggctg atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac
      961 gcgcgtagtg catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca
     1021 tcaggtcaca cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct
     1081 gttcggcgat ccagccagcc agcgcctgga tgcggtcagt cttgtcacgc agcgtaccca
     1141 gctgttgctg gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttgc
     1201 ggtcggtgtt gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac
     1261 cagagataat ttgctgcgga tctttcgatt cgatgttggc aacgaagata aagttcggca
     1321 gcagtttgcc gtcgttcgca tacaccggga agtacttctg gtcacctttc atggtgtaaa
     1381 ccagggcttc agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg
     1441 gccactccac cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc
     1501 caatcttacg cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat
     1561 cggcgatgac tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg
     1621 tgaattccgg ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca
     1681 gaatggttgc cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca
     1741 cgaagtgcac gtcgcttgca ccccagcgca tcagtttcgg gatcggcagt ttcgccagcg
     1801 aagtcgcaac catattcggc agcagtgctt cggtgctttc gcccttcaca tgggcgcgat
     1861 acagcagcca ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccac
     1921 aaccacgcgc ccaaccttct gccgctttgc tcggttttcc ttcagcgtcg aacgcctggg
     1981 caatcgccgg gccgcgtttt tcgatttcac gatctggttg tgcttccgcc aggttagcta
     2041 ctttcagcgc cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggcctg
     2101 cgttatccag ctccgcagta aagttcgcag caaaggactc tgccaggctg cgcagtgctt
     2161 ttggtggcag ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct
     2221 cttacttatc tttgttgcac atcgggaagc cgagggcttc acgggaagcg taatatgctt
     2281 ctgccactgc tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcggtgacgg
     2341 agatggcttt acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt
     2401 aggctggcag cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact
     2461 gctcaaagca ggtgaacagg aagtccacat ccgcgtattc gaagttgtaa gtggactgct
     2521 ccacttcgtt ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc
     2581 agactaggtc gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt
     2641 aggtgatctc gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga
     2701 actgcgtcac ttccatgccg tttagccaca cttcccagcc cagtccccag gcccccagag
     2761 tcgggttttc ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca
     2821 gctctttcag agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca
     2881 cctggaactg atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg
     2941 ggcgacgaga aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac
     3001 aggtcattgg gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc
     3061 agccctggcg agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat
     3121 caaacttttg catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat
     3181 acccgctgga tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa
     3241 agatgcaaaa aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta
     3301 taaccgcgca tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc
     3361 tcgccggtgt tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa
     3421 cgcagaggat caacccttac ccgttgtgct ttctgcacgg gtgacggtgt agaacaaccc
     3481 actagcacca taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct
     3541 ggtttgcagg tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc
     3601 ttaagcctct ataggctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga
     3661 tttatcggtc agactttttt taaccagata cagagacaca gatgcagccc aaaatttact
     3721 ggattgataa cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct
     3781 ggtatgtgac caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga
     3841 attctgcctc tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg
     3901 gcgaacgcag cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata
     3961 gcgcaatcgc actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga
     4021 aaaacctgct gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga
     4081 tttatctggt ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc
     4141 taatggtggt gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt
     4201 ttgaatggct gccaattaac ttatatatca atggcgatac tttttactac attctgtatg
     4261 gcatgttggg ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga
     4321 gcgccgcgct gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc
     4381 agtggcgcgg aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct
     4441 gcgcaatcgc gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac
     4501 ttggcttaat ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg
     4561 cgctgcgcac ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct
     4621 tttgcgcgac gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa
     4681 acagattagt gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt
     4741 attcgctgta ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac
     4801 gaatattctt ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa
     4861 aacctttttc actgagtaat aatgtcgcat tccacagacc taccatcagt agagcaacag
     4921 agataataaa gacagtcagg caggtcatat aataaatgga agtggtcggt atgccttcat
     4981 acttgtcccg aacggtcttt tgataagacg cagcagaaaa cagtcctaac accagtacgg
     5041 caaaataata ccctttttca tttaactgca tctctgcatt ccatagccct aacaggtagg
     5101 taacgatacc accaacgaga gctatccatg acacaatact aaaggccggt gaataggttg
     5161 atattttgtt gtccatcaca gtattccttt cattcctgaa tgtgtaagag ctttatgttg
     5221 ctcataccga gcgaaacaaa cgcatttgac caaacaagca gacaaaaaag gcgatggggt
     5281 agatcaccat ttcataagca ttgataggtg catttaaaac accgataaac aagagaccga
     5341 tagtgattaa cgcaactaac tggcagatat gggtaaaaaa attatccagt tgcccgagtt
     5401 tttctgcgcg aagatatgca tagttaccaa aagttgctgt cattaacacg ccaagaaaat
     5461 agccttttcc acttagcaat gggcatgaga tccatagccc aaccagataa accagcgccc
     5521 cgacaacaaa taatagtttt gccacagttt ttgaggtttt cataacgatc tccatatcta
     5581 ccagcgatac attacgagta accaacgaaa gacaaaactg aaaaatgcca ttaacaaatg
     5641 attttcagaa taaattcata ctaaatatta attaattagt gagatatata gatgtgaatt
     5701 atcccccacc cggtcaggca ggggataacg tttacgccat taatggcaga agttgctgat
     5761 agaggcgacg gaacgtttca cgtcgtggct gataagcggc ataacgctgc gcatctggta
     5821 gatgcgactg ttctaacggt agctgcggca acaactcaat gagcgatttc tctggattcg
     5881 ccgcaatctg cgccagcctt gctgcgccca gtgctggccc cacatccccc cccgtacggt
     5941 aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca ctacgcgccc
     6001 cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg acatccatgc
     6061 catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca ttggggccat
     6121 gttgatgagt caaaccaaag aaaaccccct tcgcctgggg attattgtgt ggtgtacgct
     6181 cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc tgttgagctg
     6241 cagcgattaa agctgggaca ttgcacaggc cggttaattt cgcggcccaa tccagacacg
     6301 atgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca tggcaaaagc
     6361 tgtgtacagc gctttctggc ttgcttaaga acccttcgct gacagcaaaa tagacccccg
     6421 acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca actgcaccag
     6481 ctgcattgtc gccaccgcct gcgacaactg gcaccgtcgc cataccccac gctttcgcaa
     6541 cttcaggtaa caaagcacca gtaatttcgc tgccttcgta taatgcgggc atctggtcac
     6601 gagataagtc gcaagcctgc agcatgacgt cactccagtc acgctttgcg acatccagcc
     6661 acatggtgcc agctgcgtca gacatatcgc tggcaaactc ccccgtcata cgcagacgca
     6721 agtaatcttt cggtaataat actttgtcga tttgacggaa tatctccggc tcatgccgct
     6781 gaacccatag caatttaggc gcagtaaatc cgggcatcat caggttgccg gtaatcactc
     6841 gtgattgcgg aactctcgct tccagcaaag tgcactcttg cgcacagcgc ccgtcgttcc
     6901 acaaaatggc agggcgcaat acccgttgtt gagcatccag taaggttgct ccatgcatct
     6961 ggccggcaat acccaatgct ttaacgtcct gcagagaatg ctgatcgccc agagctttca
     7021 ttgcgcgatc agttgcctgc caccactgtt ccgggtcttg ttccgaccaa agtggatgcg
     7081 ggcgcgaaac ggtcagcttt tccgtttgcg aagcaaccac ctcaccctgc tcgttgagca
     7141 aaataacttt tacgcccgag gtgccaagat ctatcccgat atacatatcg atcgttcctt
     7201 aaaaaaatgc ccggtatcgc taccgataac cgggccaacg gactgcacag ttagccgtta
     7261 tttgtcgaac agataatgat ttaccagatt ttccagttgc tcctggcgac cactctgatg
     7321 caccggagac aaattatgtt cctgagcata tttggctaaa tctgccagtg acatttggcc
     7381 tttcaggatt tgctggccca attcgctatt ccagccggaa taacgctgcg cgatgcgttt
     7441 atccagctcg ccatcttcaa tcatgcgcgc tgcaattttc agcgccagtg ccatcgtatc
     7501 catcgcgccg atatgaccgt aaaacagatc atatttatca gtactttgac gacgtacttt
     7561 ggcatcgaag ttcagaccac cggtggtgaa accgcctgct ttgagaattt catacatcac
     7621 cagcgcattc tcttccacac tgttcgggaa ctggtcggtg tcccagccca gttgcgcatc
     7681 gccacggttg gcgtcgacag aaccgaacag gccaagcgca atggcggtgg ctatttcatg
     7741 atggaaagag tgacctgcca gcgtcgcgtg gttagcttca atgttcagtt taatctcttt
     7801 ttccagacca aactgtttca ggaagccata gaccgtcgcg gcatcgtaat catattgatg
     7861 tttagtcggt tcttgcggtt tcggttcgat aagcaacgtg ccctagaagc cgattttatg
     7921 tttatgctca accaccatct gcataaagcg gcccagttgt tcacgctcct gacgcaagtc
     7981 ggtatttaac agcgtttcgt aaccttcacg accaccccac aggacatagt tttcaccgcc
     8041 caatttatgg gttgcttcca tcgctgtaac aacttgcgtt gccgcccagc tgaagacttc
     8101 aggatctggg ttcgtcgccg cacccgcgcc atagcgaggg tttgtaaagc agttagcggt
     8161 tccccacagc agcttcacgc cgctctcttc ttgcttggct gccaggacat caaccatttg
     8221 cgcaaaatta ttgatgtact cttttaacga cgcgccctca ggggaaacat ccacatcgtg
     8281 gaagcaataa aatggcacat gtaacttgtg gaaaaactca aatgcgacat ctgctttacg
     8341 cttcgccaac gccagtgcct caccaggctg ctgccacgga cgattaaacg cccccacacc
     8401 aaacatgtcc gccccgttcc agcagaaggt gtgccagtag caggcggcaa aacgcaagtg
     8461 ctcttccata cgcttaccca acaccagttc gtcgggattg tagtgacgga atgctaacgg
     8521 gtttgaggat tttgagcctt cataacgaac gcgatcgagc tggtcaaaat aggcttgcat
     8581 attgaactcc ataatcaggt aatgccgcgg gtgatggatg atgtcgtaat attgggcact
     8641 ccctttcaat tgctcaatta tgttatttca cactgctatt gagataattc acaagtgtgc
     8701 gctcgctcgc aaaataaaat gggatgatga aaatgggtaa ttcctcgaag ataaaaatgc
     8761 aataagtaca attgcgcaac aaaagtaaga tctcggtcat aaatcaagaa ataaaccaaa
     8821 aatcgtaatc gaaagataaa aatctgtaat tgttttcccc tgtttagttg ctaaaaattg
     8881 gttacgttta tcgcggtgat tgttacttat taaagctgtc ctctaactac agaaggccct
     8941 acaccatgaa aataaagaac attctactca ccctttgcac ctcacttctg cttaccaacg
     9001 ttgcggcaca cgccaaagaa gtcaaaatag gtatggcgat tgatgatctc cgtcttgaac
     9061 gctggcaaaa agatcgagat atttttgtga aaaaggcaga atctctcggt gcgaaagtat
     9121 ttgtacagtc tgcaaatggc aatgaagaaa cacaaatgtc gcagattgaa aacatgatta
     9181 accggggtgt cgatgttctt gtcattattc cgtataacgg tcaggtatta agtaacgttg
     9241 taaaaaaagc caaacaagaa ggtattaaag tattagctta cgaccgtatg attaacgatg
     9301 cggatatcga tttttatatt tctttcgata acgaaaaagt cggtgaactg caggcaaaag
     9361 ccttggtcga tattgttccg caaggtaatt acttcctgat gggcggctcg ccggtagata
     9421 acaacgccaa gctgttccgc gccggacaaa tgaaagtgtt aaaaccttac gttgattctg
     9481 gaaaaattaa agtcgttggt gaccaatggg ttgatggctg gttaccggaa aacgcattga
     9541 aaattatgga aaacgcgcta accgccaata ataacaaaat tgatgctgta gttgcctcaa
     9601 acgatgccac cgcaggtggg gcaattcagg cattaagcgc gcaaggttta tcagggaaag
     9661 tagcaatttc cggccaggat gcggatctcg caggtattaa acgtattgct gccggtacgc
     9721 aaactatgac ggtgtataaa cctattacat tgctggcaaa tactgccgca gaaattgccg
     9781 ttgaattggg caatggtcag gagccaaaag cagatacctc actgaataat ggcctgaaag
     9841 atgtcccctc ccgcctcctg acaccgatcg atgtgaataa aaacaacatc aaagatacgg
     9901 taattaaaga cggattccac aaagagagcg agctgtaagc gttacgcccc ggcgcggagc
     9961 gggggcgtga tttctctcca tgccgcgtga atgaattggc ttaggtggag tcgttatgcc
    10021 ttatctactt gaaatgaaga acattaccaa aaccttcggc agtgtgaagg cgattgataa
    10081 cgtcagcttg cggttgaatg ctggcgaaat cgtctcactc tgtggggaaa atgggtctgg
    10141 taaatcaacg ctgatgaaag tgctgtgtgg tatttatccc catggctcct acgaaggcga
    10201 aattattttt gcgggagaag agattcaggc gagtcacatc cgcgataccg aacgcaaagg
    10261 tatcgccatc attcaccagg aattggccct ggtgaaagaa ttgaccgtgc tggaaaatat
    10321 cttcctgggt aacgaaataa cccacaatgg cattatggat tatgacctga tgacgctacg
    10381 ctatcagaag ctgctcgcac aggtcagttt atccatttca cctgataccc gcgttggcga
    10441 tttagggctt gggcaacaac aactggttga aattgccaag gcacttaata aacaggtgcg
    10501 cttgttaatt ctcgatgaac cgacagcctc attaactgag caggaaacgt cggttttact
    10561 ggatattatt cgcgatctac aacagcacgg tatcgcctgt atttatattt cgcacaaact
    10621 caacgaagtc aaagcgattt ccgatacgat ttgcgttatt cgcgacggac agcacattgg
    10681 tacgcgtgat gctgccggaa tgagtgaaga cgatattatc accatgatgg tcgagcgaga
    10741 gttaaccgcg atttacccta atgaaccaca taccaccgga gatgaaatat tacgtattga
    10801 acatctgacg gcatggcatc cggtcaatcg tcatattaaa cgagttaatg atgtctcgtt
    10861 ttccctgaaa cgtggcgaaa tactgggtat tgccggactc gttggtgccg gacgtaccga
    10921 gaccattcag tgcctgtttg gcgtgtggcc cggacaatgg gaaggaaaaa tttatattga
    10981 tggcaaacag gtagatattc gtaactgtca gcaagccatc acccagggga ttgcgatggt
    11041 acccgaagac agaaagcgcg acggcatcgt tccggtaatg gcggttggta aaaatattac
    11101 cctcgccgca ctcaataaat ttaccggtgg cattagccag cttgatgacg cggcagagca
    11161 aaaatgtatt ctggaatcaa ttcagcaact caaagttaaa acgtcgtccc ccgaccttgc
    11221 tattggacgt ttgagcggcg gcaatcagca aaaagcgatc ctcgcccgct gtctgttact
    11281 taacccgcgc atcctcattc ttgatgaacc taccaggggt attgatattg gcgcgaaata
    11341 tgagatctac aaattaatta accaactcgt ccagcagggt attgccgtta ttgtcatctc
    11401 ttccgaatta cctgaagtgc tcggccttag cgatcgtgta ctggtgatgc atgaagggaa
    11461 actaaaagcc aacctgataa atcataacct gactcaggag caggtgatgg aagccgcatt
    11521 gaggagcgaa catcatgtcg aaaagcaatc cgtctgaagt gaaattggcc gtaccgacat
    11581 ccggtggctt ttctgggctg aaatcactga atttgcaggt cttcgtgatg attgcagcta
    11641 tcatcgcaat catgctgttc tttacctgga ccaccgatgg tgcctactta agcgcccgta
    11701 acgtctccaa cctgttacgc cagaccgcga ttaccggcat cctcgcggta ggaatggtgt
    11761 tcgtcataat ttctgctgaa atcgaccttt ccgtcggctc aatgatgggg ctattaggtg
    11821 gcgtcgcggc gatttgtgac gtctggttag gctggccttt gccacttacc atcattgtga
    11881 cgttggttct gggactgctt ctcggtgcct ggaacggatg gtgggtcgcg taccgcaaag
    11941 tcccttcatt tattgtcacc ctcgcgggca tgttggcatt tcgcggcata ctcattggca
    12001 tcaccaacgg cacgactgta tcccccacca gcgccgcgat gtcacaaatt gggcaaagct
    12061 atctccccgc cagtaccggc ttcatcattg gcgcgcttgg cttaatggct tttgttggtt
    12121 ggcaatggcg cggaagaatg cgccgtcagg ctttgggttt acagtctccg gcctctaccg
    12181 cagtagtcgg tcgccaagct ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga
    12241 atgattaccg tggcgttccc actcctgttc tgctgctgac gttgctgtta ctcggcggaa
    12301 tgtttatggc aacgcggacg gcatttggac gacgcattta tgccatcggc ggcaatctgg
    12361 aagcagcacg tctctccggg attaacgttg aacgcaccaa acttgccgtg ttcgcgatta
    12421 acggattaat ggtagccatc gccggattaa tccttagttc tcgacttggc gctggttcac
    12481 cttctgcggg aaatatcgcc gaactggacg caattgcagc atgcgtgatt ggcggcacca
    12541 gcctggctgg cggtgtggga agcgttgccg gagcagtaat gggggcattt atcatggctt
    12601 cactggataa cggcatgagt atgatggatg taccgacctt ctggcagtat atcgttaaag
    12661 gtgcgattct gttgctggca gtatggatgg actccgcaac caaacgccgt tcttgatttt
    12721 gataaaaatt ttctcaaagc cggttacgta ttaccggttt tgagtttttg catgattcag
    12781 caggaaaaga accatgttta ctaaacgtca ccgcatcaca ttactgttca atgccaataa
    12841 agcctatgac cggcaggtag tagaaggcgt aggggaatat ttacaggcat cacaatcaga
    12901 atgggatatt ttcattgaag aagatttccg cgcccgcatt gataaaatca aggactggtt
    12961 aggagatgtc gtcattgccg acttcgacga caaacagatc gagcaagcgc tggctgatgt
    13021 cgacgtcccc attgttgggg ttggcggctc gtatcacctt gcagaaagtt acccacccgt
    13081 tcattacatt gccaccgata actatgcgct ggttgaaagc gcatttttgc atttaaaaga
    13141 gaaaggcgtt aaccgctttg ctttttatgg tcttccggaa tcaagcggca aacgttgggc
    13201 cactgaacgc gaatatgcat ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt
    13261 ttatcagggg ttagaaaccg cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga
    13321 ctgactacaa acgctgccac cgcaaaccgg gattattgcc gttactgacg cccgagcgcg
    13381 gcatattctg caagtatgtg aacatctaca tattcccgta ccggaaaaat tatgcgtgat
    13441 tggcatcgat aacgaagaac tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc
    13501 tcagggcgcg cggcaaatgg gctatcaggc ggcaaaactg ttgcatcgat tattagataa
    13561 agaagaaatg ccgctacagc gaattttggt cccaccagtt cgcgtcattg aacggcgctc
    13621 aacagattat cgctcgctga ccgatcccgc cgttattcag gccatgcatt acattcgtaa
    13681 tcacgcctgt aaagggatta aagtggatca ggtactggat gcggtcggga tctcgcgctc
    13741 caatcttgag aagcgtttta aagaagaggt gggtgaaacc atccatgcaa tgattcatgc
    13801 cgagaagctg gagaaagcgc gcagtctgct gatttcaacc accttgtcga tcaatgagat
    13861 atcgcaaatg tgcggttatc catcgctgca atatttctac tctgttttta aaaaagcata
    13921 tgacacaacg ccaaaagagt atcgcgatgt aaatagcgag gtcatgttgt agcgatgatg
    13981 agaattgtcg gcgtcacatc agggaactca gcacagccac ctgatgcgag caaaagcctt
    14041 atccgaccga caaatcagat aaggctcgag gaaatgaaat tacatatgcg ccgcgattaa
    14101 ccgttggtta tcctggtaca ttgcgaacag gtagttgtta taactcttcc ctttggtcga
    14161 gtagcccttc agcttgtgaa tcatcgctgt ggcagtcact tcctgatccg ctttacgcag
    14221 ctgtgcacgc gatttacgga acgaagagta agccgggtgc gtattcaggt tagtgacata
    14281 ggcgctcacc gattctttga cagaactaaa ctgtgagtac cctttcactt tacccggcgc
    14341 attggtacaa cgtcctttca tgcatttcat gccgaacaga ttgttattgt tgcgcgccag
    14401 cttcgacgtt ccccaaccgc tttctgctgc agccatcgtc gccaccatac tggtagggat
    14461 aatgtctacg cgttcaagca aggtattcca cgggattttt cgcgtattac cagaccactt
    14521 caccttgtag cgtttggcga tgtctttcag acgcgcacgc tcagcaggtg cccattggcc
    14581 ctgatactgt tttgaaatca gccagttacg ttctgcagta atggctgcat tttggctggt
    14641 aatgtaaggc attacggtcc ggagaaacgc ctttttcctt ggtgttccgg aagggtattt
    14701 tcgcaaatca ggaagtgaac tactctttgc actattgcga gaatactctt gtttactgct
    14761 tacctgttta ttactagctt tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt
    14821 cttcgctaac acctcactcg aaaacaccag agtgagtaac ataagaatca tcgccccata
    14881 tcgtcgtatg ggagtcaaaa tcatcaggtc tcctggtcgg atttaatcat tccaacacct
    14941 tatatttttc acaaatttga gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag
    15001 caccccaagg aatagtccaa atctgaaact atgtcacgag ttaacgattc agattggcgc
    15061 taaatcgcag aaaatgtggg ggttatcgca aaattcagcc gttttttgcg cgcgatcgct
    15121 cacccttgct tctcatcctg tggacttacc gctcagggat gagttttgtc tggcttatcg
    15181 ctggcaaact gtctgaaatc gcagcaataa ggactcatcc accatgaaac tcgccgcctg
    15241 ttttctgaca ctccttcctg gcttcgccgt tgccgccagc tggacttctc cggggttccc
    15301 tgcctttagc gaacagggaa cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac
    15361 gcgtccactc acgctaaatt ttgaccaaca gtgctggcag cctgcagatg cgataaaact
    15421 caatcagatg ctttccctgc aaccttgtag caacacgccg cctcaatggc gattgttcag
    15481 ggacggcaaa tatacgctgc aaatagacac ccgctccggt acgccaacat tgatgatttc
    15541 catccagaac gccgccgaac cggtagcaaa cctggtccgt gaatgcccga aatgggatgg
    15601 attaccgctc acgctggatg tcagcgccac tttcccggaa ggagccgccg tacgggatta
    15661 ttacagccaa caaattgcga tagtgaagaa cggtcaaata acgttacaac ccgctgctac
    15721 cagcaacggt ttactcctgc tggaacgggc agaaactgac acatccgccc ctttcgactg
    15781 gcataacgcc acggtttact ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa
    15841 tgaccagagt tacggacgtc ataaagacgg tatggcggaa attggcactt ttcacggcgg
    15901 cgatttacgc ggcctgacca acaaactgga ttacctccag cagttgggcg ttaatgcttt
    15961 atggataagc gccccatttg agcaaattca cggctgggtc ggcggtggta caaaaggcga
    16021 tttcccgcat tatgcctacc acggttatta cacacaggac tggacgaatc ttgatgccaa
    16081 tatgggcaac gaagccgatc tacggacgct ggttgatagc gcacatcagc gcggtattcg
    16141 tattctcttt gatgtcgtga tgaaccacac cagctatgcc acgctggcgg atatgcagga
    16201 gtatcagttt ggcgcgttat atctttctgg tgacgaagtg aaaaaaacgc tgggtgaacg
    16261 ctggagcgac tggaaacctg ccgccggaca aacctggcat agctttaacg attacattaa
    16321 tttcagcgac aaaacaggct gggataaatg gtggggaaaa aactggatca gaacggatat
    16381 cggcgattac gacaatcctg gattcgatga tctcactatg tcgctggcct ttttgccgga
    16441 tatcaaaacc gaatcaacta ccgcttctgg tctgccggtg ttctataaaa acaaaacgga
    16501 tactcacgct aaagtcatcg aaggctttac acctcgcgat tacttaaccc actggttaag
    16561 tcagtgggtc cgcgactatg ggattgatgg ttttcgggtc gataccgcca aacatgttga
    16621 gttgcccgcc tggcagcaac tgaaaaccga agccagctcc gcgcttcgcg aatggaaaaa
    16681 aactaacccc gacaaagcat tagatgacaa acctttctgg atgaccggtg aagcctgggg
    16741 ccacggcgtg atgcaaagtg actactatcg ccacggcttc gatgcgatga tcaatttcga
    16801 ttatcaggag caggcggcga aagcagtcga ttgtctggcg cagatggata cgacctggca
    16861 gcaaatggcg gagaaattgc agggtttcaa cgtgttgagc tacctctcgt cgcatgatac
    16921 ccgtctgttc cgtgaagggg gcgacaaagc agcagagtta ttactattag cgccaggcgc
    16981 ggtacaaatc ttttatggcg atgaatcctc gcgtccgttc ggtcctacag gttctgatcc
    17041 gctgcaaggt acacgttcgg atatgaactg gcaggatgtt agcggtaaat ctgccgccaa
    17101 cgtcgcgcac tggcagaaaa tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg
    17161 caaacaaacg acactttcgc tgaagcaggg ctacggcttt gttcgtgagc atggcgacga
    17221 taaagtgctg gtcatctggg ctgggcaaca gtgacttttc cggcttcccg ttcgtcagta
    17281 cctcgggaag ccgccaacca ggataaaatg tcagccctaa tcagcgttgc gggataaagc
    17341 accgctcact cttcaacaga ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt
    17401 ccttgctaca gagttcgaca gatatcccgc tatgacattc tccctttttg gtgacaaatt
    17461 tacccgccac tccggcatta cgctgctgat ggaagatctg aacgacggtt tacgcacgcc
    17521 tggcgcgatt atgctcggcg gcggtaatcc ggcgcagatc ccggaaatgc aggactactt
    17581 ccagacgcta ctgaccgaca tgctggaaag tggcaaagcg actgatgcac tgtgtaacta
    17641 cgacggtcca caggggaaaa cggagctact cacactgctt gccggaatgc tgcgcgagaa
    17701 gttgggttgg gatatcgaac cacagaatat tgcactaaca aacggcagcc agagcgcgtt
    17761 tttctactta tttaacctgt ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct
    17821 gttcccgctg gcaccggaat acattggcta cgctgacgcc ggactggaag aagatctgtt
    17881 tgtctctgcg cgtccgaata ttgaactgct gccggaaggc cagtttaaat accacgtcga
    17941 ttttgagcat ctgcatattg gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa
    18001 tccaacaggt aatgtgatta ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca
    18061 acacggcatt ccgctggtga ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt
    18121 cagtgaaggg cgcccgctat ggaatccgaa tatcgtactg tgcatgagtc tttccaagct
    18181 ggggctgcct ggctcccgct gcggcattat catcgccaat gaaaaaatca tcactgccat
    18241 caccaatatg aacggcatta tcagcctggc acctggcggt attggtccgg cgatgatgtg
    18301 tgaaatgatt aagcgtaacg atctgctgcg cctgtctgaa acagtcatca aaccgtttta
    18361 ctaccagcgt gttcaggaaa ctatcgccat cattcgccgc tatttaccgg aaaatcgctg
    18421 cctgattcat aaaccggaag gagccatttt cctctggcta tggtttaagg atttgcccat
    18481 tacgaccgag cagctctatc agcgcctgaa agcacgcggc gtgctgatgg tgccggggca
    18541 caacttcttc ccgggactgg ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa
    18601 ctacgtacca gagccggaga aaattgaggc gggggtgaag attctggcgg aagagataga
    18661 aagagcctgg gctgaaagtc actaaaaatt gcctgatgcg ctacgcttat caggcctaca
    18721 tgatttctgc aatatattga atttggaaga atttgtaggc cggataaggc gtttacgccg
    18781 catctggcat aaacaaagcg cactattccg gcattgagaa acgccggaaa acgtttcaaa
    18841 caggctgctg ctgtagccga tgccgctgca ctttctcgac atccatgcac tgcaacgcct
    18901 ttgtcgggca ggcttcaaca caggccggcc ccgtctcccg atgccagcac agatcgcact
    18961 taatcgccct cgcctttttg cgcgaagaaa cgacctccat cgcaccaaac gggcaagcca
    19021 gcatacagct tttacagcca atgcaacgtg tttgttcaac gaaaatatgc ccatgttcgc
    19081 ggcttatcgc gtcaacaggg cagacattcg cgcacggtgc atcttcacac tgatgacagg
    19141 ctactggcgt ggtccagctg tggtctttaa tgacacgaat acgggaaata aactcgtctg
    19201 gtgacaacgc agcgcaatcc tgattctcct gatgcgacac tgcgcaagcc acttcacagg
    19261 tacggcaacc gatacatttc gtcgcatccg caataataaa ccggttcatg atcattcctt
    19321 tgcactaaaa cagcagagtg ccagagagcg cgtccctact gctttgatcc aacgaaggaa
    19381 atgttaaagt tttggcactt gtccaaagcg ttatgtgatc gcgcccaggt catccctgac
    19441 agtaaatccc agttcattag aaatagcctg cgcggtttca cgcagtggtt tcaggagatt
    19501 tttctctccc acctgtttca gacgtgatgt cgaaagcgaa atcgacacgg cgtacggcac
    19561 ccgcccatga atatcaaaca ccggaacagc aatacaggag acgccgagtt cgttttcttc
    19621 tctgtccatc gccgctccgc tttcacgaat gtgcgccagt tcgtcgaaca tcgcgggcag
    19681 ctcggtaatg gtattgcggg ttaacggctg gatctcatgc tgatggtttt cccagtatga
    19741 cttcacgtag tccgggtgac caaacgccat gtagatcttg cccattgcgg aacagtagag
    19801 cggcatatgc tggccaatat aggcacgggt tcgcagcatc ccggttgtgg gttccagctt
    19861 ataaatcaaa atagcgtgat cgtcttcgcg gctggagaag ttaatggttt caccagtggc
    19921 gatgttcagt gcctcaagat gcggagcggc gatatgaatg atattcagcg aagacagcgc
    19981 cttctgcccg acggcaataa atttggtagt caggcggtaa ctccctgcgg cgggcgcggt
    20041 ggtcacatag ccgcaggact gtaatccctg caataagcga tggacggtac tcttatttaa
    20101 accagccagc tccgaaagat gcgccaacgg acaaccgttt ggatagttgc tcaaaatctc
    20161 aatcagcatc aacccgcgaa acagactctg gcttccggct ggacgctctt tttcctgcgc
    20221 catctcgttc tctttttttc ccatcacttc tttccccatt ttgtcgcgtc ctgatggtag
    20281 cgcaaagtgt gccgtagttc acgatctcga cagataattt ataaccactt gatttttatg
    20341 tcttttgaaa ttcatcaatc agattgcttt gttaaaaagt gatcgatata tttgaaatct
    20401 agttccgcat attgaaattt taagccaaaa aagcgatcaa aaaacaagga agcctaggat
    20461 gaaagtgaca tttgagcagt taaaagcagc ctttaatcgg gtcttaattt cacgcggcgt
    20521 tgacagcgaa acggctgacg cctgtgcaga gatgttcgcc cgcaccaccg aatccggcgt
    20581 ttattctcac ggcgttaatc gtttccctcg tttcattcaa caactggaaa acggcgatat
    20641 cattcctgat gcccaaccca aacgtataac cagcctcggc gcaattgaac agtgggatgc
    20701 ccagcgttcg atcggcaacc tgacggcgaa aaagatgatg gatcgcgcca ttgaactggc
    20761 tgccgatcac ggtattggtc tggtggcact tcgtaatgcc aaccactgga tgcgcggcgg
    20821 cagctacggc tggcaggcgg cggaaaaagg ctatattggc atttgctgga ccaactccat
    20881 cgccgtaatg ccgccgtggg gcgcaaaaga gtgtcgcatc ggcactaacc cgctgatcgt
    20941 cgccattcct tccacgccga tcaccatggt cgatatgtcg atgtcgatgt tctcttacgg
    21001 catgttagaa gttaaccgcc tggcaggccg tcagctcccg gtcgatggtg gctttgatga
    21061 tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca ttttgccgat
    21121 gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcta ctctcctttc
    21181 cgacggcgca tcggttgccg aagtcaccca ggacaacagc gacgaatacg gcatttcaca
    21241 aatttttatt gccattgaag tggacaagct tatcgacggt cccacccgcg atgccaagct
    21301 gcaacgtatc atggattacg ttactaccgc tgaacgcgct gacgaaaacc aggccatccg
    21361 cttacccggc cacgaattta ctaccctgct ggccgaaaac cgccgtaacg gcatcactgt
    21421 tgatgacagc gtgtgggcca aaatccaggc gttatgagga gataagtcat gatttttgga
    21481 catatcgcgc agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc gctcgatttt
    21541 ctgcgcgcta ccaacttcaa cgtcctggag ccgggcgttg tcgaaatcga cggcaagaat
    21601 atttatgcgc
//
LOCUS       DAJVQR010000066        21513 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVQR010000066 DAJVQR010000000
VERSION     DAJVQR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OQM52_002641"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OQM52_002641"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1564442.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="OQM52_002642"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="OQM52_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1564443.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="OQM52_002643"
     CDS             complement(2650..3201)
                     /locus_tag="OQM52_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1564444.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="OQM52_002644"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="OQM52_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1564445.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="OQM52_002645"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="OQM52_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OQM52_002646"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OQM52_002646"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1564446.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OQM52_002647"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OQM52_002647"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1564447.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OQM52_002648"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OQM52_002648"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1564448.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OQM52_002649"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OQM52_002649"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1564449.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OQM52_002650"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OQM52_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1564450.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="OQM52_002651"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="OQM52_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1564451.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="OQM52_002652"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="OQM52_002652"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1564452.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OQM52_002653"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OQM52_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1564453.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OQM52_002654"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OQM52_002654"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1564454.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OQM52_002655"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OQM52_002655"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1564455.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OQM52_002656"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OQM52_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1564456.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18171..18566)
                     /locus_tag="OQM52_002657"
     CDS             complement(18171..18566)
                     /locus_tag="OQM52_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1564457.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OQM52_002658"
     CDS             complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OQM52_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1564458.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19482..20075
                     /locus_tag="OQM52_002659"
     CDS             19482..20075
                     /locus_tag="OQM52_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1564459.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20220..20672
                     /gene="tabA"
                     /locus_tag="OQM52_002660"
     CDS             20220..20672
                     /gene="tabA"
                     /locus_tag="OQM52_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1564460.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20795..>21513
                     /locus_tag="OQM52_002661"
     CDS             20795..>21513
                     /locus_tag="OQM52_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1564461.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5196 a         5324 c         5668 g         5325 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggcaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcga cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaagcggtg
    17941 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat
    18001 atatgcattt ttattttcta tgattctgga ggggaagtgt cgttcggtat caataattgc
    18061 agacatttcc tgcttttttc tttttcattt tcacgcaatc agaaatgcat tttattccat
    18121 tcatccgact tatagggcgg agggtgaaaa cgaacggcta acactattgc ttatagctca
    18181 gggatacgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacacccacc
    18241 gccgtccagt ttggataagg tggggcgcta aatatttcat ttttcaccgt catgatgtct
    18301 tcagattgtt tttccggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    18361 catcccgcag ctgccagggt cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga
    18421 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    18481 tcgccggaac gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca
    18541 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    18601 gatttatatt tatcgggagc cgcttttagg gggacgacgt tagtaaacaa aacccagaca
    18661 tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac gcgccatcaa
    18721 tggtatgcat cgcgccggta acaaaactgg cttctggccc tgctaaccat gcgaccatac
    18781 cagcgacctc ttccggttgc ccatgtcttt tgatggccat aaaactatgc aacatatcgc
    18841 gcattggccc gttggcggga ttaacgtcgg tatcaattgg tcctggctgg acgacgttga
    18901 tggtgatccc tcgcggtcca aaatcacggg ccagcccgcg cgccatgcct tgcagggcag
    18961 atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca ttcacggagc
    19021 cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaaca gaggcatgat
    19081 aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt aattccaggg
    19141 catcgccaaa gacgccaata cctgcattta ccaccaggat atccaatgcg ccgctcttac
    19201 gaacgacatc aataacagcg tctctgtcag cactatctgt gaatactgct gtcgctccag
    19261 tctcttgtgc cagacgttca gcggcatctt tcgaccccgc ataggtgaat cgtacattgg
    19321 ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacgactg ccaccgagga
    19381 tgagaactgt cttaccggta aaagcgccca taaggactcc ttgatttatt atgtaacatg
    19441 cattacaaaa ctgttttaac tttctgtcaa caggttttgt aatggtcact aaaaaacaat
    19501 ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct gcggcaaaag
    19561 tgctttttca ccaaaaaggt ttcgatgcag taagtgtctc tgaagttact gattatcttg
    19621 gtattaaccc cccgagtctc tacgcggctt ttggcagtaa ggctgggtta tttagccgtg
    19681 tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt cgtgatgatc
    19741 gtccggtagg cgagtgcctg gttgaggtat taaaagaagc ggcgcgcaga tatagccaaa
    19801 acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat ccacaagcgc
    19861 gtgatattgc cattcaatat tatcacgccg cagaaacgac catttatgac tatatcgcca
    19921 ggcggcatcc acaaagcgca caatgtgtga ctgattttat gagtaccgtg atgtcagggc
    19981 tttctgcgaa ggcacgggag gggcactcca tcgaacaact ctgtgcaaca gctgccctgg
    20041 caggggaagc gataaaaact cttctcaagg agtgatgctg gccttgatcc gaaaggcggg
    20101 aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc tggcggaatt
    20161 gatcatcttt tcgtataacg atagaaatga aacattgttt taattaagga gtggaaaaga
    20221 tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta cgccaggcga
    20281 ttgagcatat caaagcacac gttacggcag aaacgccaaa gggcaagcat gatatcgaag
    20341 gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa gctcgccgtg
    20401 cggagtacca tgcccgctat cttgacattc agattgtgtt aaaaggtcag gaaggcatga
    20461 ccttcagcac gcaacctgca ggcgcgccgg ataccgactg gttagctgat aaagacatcg
    20521 catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt gattttgttg
    20581 tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggcgcgcca gcccaggttc
    20641 gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat aactccaggt
    20701 ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa aatttgtaat
    20761 gcttagacgt tcttattaac tcaaggagtt cgtcatgggc aaaatatcag atttgaatta
    20821 ttctcaacac attacattag ccgacaattt taaacaaaaa aatgaagcct tagatacctg
    20881 gtatgtggga atgaatgatt ttgcccgaat tgccgggggg cagaatagca gaagcaatat
    20941 tctttctcct agagcatttt tggagttttt ggcaaagata tttaccctgg gttatgtgga
    21001 ttttagcaaa cgctccaacg aagcgggtag aaatatgatg gctcatattg agttctcatc
    21061 ttattctaaa gatactgatg gcaatgaaaa aatgaagttt tacatgaata atcctgaagg
    21121 ggagcgagcg gatttatcta aggtgaagat agaaattaca cttgcctcag cttctactaa
    21181 agggattcgt gaaggacata cagtcattat atttaaacaa tctgatggtt cgactaatcg
    21241 ttatgaaggg aagtcttttg aaagaaaaga tgatagttca ttacacctga ttactaacaa
    21301 ggttctggcg tgttaccaac gcgaagctaa caagaaaata gcgcgtctat taaataatca
    21361 tcagaagtta aataatatac aggagttaaa tgattcgcag gagttaaata attcacagaa
    21421 gttaaataat tcgcaggagt taaataactc gcaggtgagt tgtaaaggtt cagttgattc
    21481 tacgattacg gatttattag aaaaagcatt gaa
//
LOCUS       DAJVQR010000067        21275 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVQR010000067 DAJVQR010000000
VERSION     DAJVQR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..444)
                     /locus_tag="OQM52_002662"
     CDS             complement(<1..444)
                     /locus_tag="OQM52_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1564462.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OQM52_002663"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OQM52_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1564463.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OQM52_002664"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OQM52_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1564464.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OQM52_002665"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OQM52_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1564465.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="OQM52_002666"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="OQM52_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1564466.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="OQM52_002667"
     CDS             complement(5214..6812)
                     /locus_tag="OQM52_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1564467.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OQM52_002668"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OQM52_002668"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1564468.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="OQM52_002669"
     CDS             complement(8491..9069)
                     /locus_tag="OQM52_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1564469.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OQM52_002670"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OQM52_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1564470.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="OQM52_002671"
     CDS             10508..10687
                     /locus_tag="OQM52_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1564471.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="OQM52_002672"
     CDS             complement(10807..11166)
                     /locus_tag="OQM52_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1564472.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="OQM52_002673"
     CDS             complement(11528..11872)
                     /locus_tag="OQM52_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1564473.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="OQM52_002674"
     CDS             12004..13914
                     /locus_tag="OQM52_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1564474.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="OQM52_002675"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="OQM52_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1564475.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="OQM52_002676"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="OQM52_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1564476.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="OQM52_002677"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="OQM52_002677"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1564477.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="OQM52_002678"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="OQM52_002678"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1564478.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OQM52_002679"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OQM52_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1564479.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OQM52_002680"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OQM52_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20410..20976)
                     /locus_tag="OQM52_002681"
     CDS             complement(20410..20976)
                     /locus_tag="OQM52_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1564480.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21275
                     /locus_tag="OQM52_002682"
     CDS             21188..>21275
                     /locus_tag="OQM52_002682"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1564481.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5265 a         5272 c         5465 g         5273 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg tatttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt tttctgcgct
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DAJVQR010000068        21264 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVQR010000068 DAJVQR010000000
VERSION     DAJVQR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            15..923
                     /locus_tag="OQM52_002683"
     CDS             15..923
                     /locus_tag="OQM52_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564482.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            1162..1221
                     /locus_tag="OQM52_002684"
     CDS             1162..1221
                     /locus_tag="OQM52_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1564483.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1538..1597
                     /gene="ibsE"
                     /locus_tag="OQM52_002685"
     CDS             1538..1597
                     /gene="ibsE"
                     /locus_tag="OQM52_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1564484.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="OQM52_002686"
     CDS             complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="OQM52_002686"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1564485.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="OQM52_002687"
     CDS             complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="OQM52_002687"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1564486.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6061..7362)
                     /gene="ygiF"
                     /locus_tag="OQM52_002688"
     CDS             complement(6061..7362)
                     /gene="ygiF"
                     /locus_tag="OQM52_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1564487.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7604..8224
                     /locus_tag="OQM52_002689"
     CDS             7604..8224
                     /locus_tag="OQM52_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1564488.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8288..9526
                     /gene="cca"
                     /locus_tag="OQM52_002690"
     CDS             8288..9526
                     /gene="cca"
                     /locus_tag="OQM52_002690"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1564489.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="OQM52_002691"
     CDS             complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="OQM52_002691"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1564490.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10601..10969)
                     /gene="folB"
                     /locus_tag="OQM52_002692"
     CDS             complement(10601..10969)
                     /gene="folB"
                     /locus_tag="OQM52_002692"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1564491.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11074..11691
                     /gene="plsY"
                     /locus_tag="OQM52_002693"
     CDS             11074..11691
                     /gene="plsY"
                     /locus_tag="OQM52_002693"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1564492.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11704..12635)
                     /gene="ttdR"
                     /locus_tag="OQM52_002694"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11704..12635)
                     /gene="ttdR"
                     /locus_tag="OQM52_002694"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12842..13685
                     /gene="ttdA"
                     /locus_tag="OQM52_002695"
                     /pseudo
     CDS             12842..13685
                     /gene="ttdA"
                     /locus_tag="OQM52_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13682..14287
                     /gene="ttdB"
                     /locus_tag="OQM52_002696"
     CDS             13682..14287
                     /gene="ttdB"
                     /locus_tag="OQM52_002696"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1564493.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14335..15798
                     /gene="ttdT"
                     /locus_tag="OQM52_002697"
     CDS             14335..15798
                     /gene="ttdT"
                     /locus_tag="OQM52_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1564494.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="OQM52_002698"
     CDS             complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="OQM52_002698"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1564495.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17092..17307
                     /gene="rpsU"
                     /locus_tag="OQM52_002699"
     CDS             17092..17307
                     /gene="rpsU"
                     /locus_tag="OQM52_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1564496.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17418..19163
                     /gene="dnaG"
                     /locus_tag="OQM52_002700"
     CDS             17418..19163
                     /gene="dnaG"
                     /locus_tag="OQM52_002700"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1564497.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19358..21199
                     /gene="rpoD"
                     /locus_tag="OQM52_002701"
     CDS             19358..21199
                     /gene="rpoD"
                     /locus_tag="OQM52_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1564498.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5130 a         5878 c         5459 g         4797 t
ORIGIN      
        1 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
       61 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      121 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      181 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg
      241 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
      301 cagaagcgcg tgctaacctt gcactcgcag aagcagtaag tgcgcaacaa aacgtagaaa
      361 ccactcgcca gaccgcagaa gccgatcgtg ctaaacaagt tgccctaatc gctgccgcgc
      421 aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg
      481 cagagatgca ggcggcggct atcgttgagt tagccgaagc tacacataaa aagggtctgg
      541 cggaagcaga agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa
      601 ccagccttaa attcaaactg gccttgttgc aggcgctacc tgcggtaata gaaaaatccg
      661 ttgagccgat gaaatctatt gacggtatca agattattca ggtcgatggt ctgaatagtg
      721 gcagcgctgc gggtgatgca aacacgggta atgtgggggg cggaaaccta gcggaacaag
      781 cattatcagc cgctctctct taccgcacac aggctccgct gattgactcc ttgctcaatg
      841 aaattggcgt ttcaggcggt tcactggcgg cattgacttc acccttaatc tcaacaactc
      901 ccgtcgccga aaacgtaaaa taaattttca gctatgaacg tccctggcac aaaaagccgg
      961 ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt gcaacgtttt
     1021 gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag cgttgcgcga ggtccggcgc
     1081 tcatcaacaa tcggggagtc agcaaagggc tgaaacggga aagcccctcc cgaggaaggg
     1141 gccttacaga aggaaagggt tatgatgaag ctcgtcatca tactgattgt gttgttactc
     1201 gtaagtttcg cagcttatta acagccaatc agagggggga gaaatcctcc ctcacccttg
     1261 ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa cacatatccc
     1321 tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgctcag
     1381 gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt
     1441 gcgcggggtc cggcgctcat caacaatcgg ggagtcagca aggggctgaa acgggaaagc
     1501 ccctcccgag gaaggggcct tatagaagga aagggttatg atgaagttcg tcatcatact
     1561 ggttgtgttg ttactgttaa gtttcccgac ttactaacaa ctgatcagag gggggagaaa
     1621 tcctccctta cccttgttcc tttactttag gttgaaaaaa caacagcgtc aataggcctg
     1681 cctgctacga agcgagatct gtgaaccgct ttccggttag ccttttttat cctgttggat
     1741 cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca gcacttcgcc
     1801 gccgttggcc cagacttctt tactcccggc gatctcttct ggtttatagt cgccgccctt
     1861 caccagtaaa tctggcagga tcccggcgat caagcgctgc ggcgtgtctt cttcaaacga
     1921 caccacccag tcgaccgctt ccagtgcgcc cagcacaatc atacgctgtt cgagcgggtt
     1981 aacggggcga gaatcccctt tcagccgttt ggtggaggca tcgctgttca cagcgacaat
     2041 caagcggtca cccagtttgc gggcatttgc cagatagaag acgtgtccgg cgtgcaggat
     2101 gtcaaagacg ccattggtca tcaccacttt ttcaccgcgt ttacgcgctg ccgctacggc
     2161 cagcttcagt tcctcttcgg tcatcacgcc aaagccggta tctgcgcgcc cacgcactgc
     2221 attttccagc tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca ccacgccagc
     2281 cgccgcattg gcaaagaagc aggcttcttc cagcgaatta cccgctgcca gcgttgccgc
     2341 caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca tacacttcct gcgcctgggt
     2401 tggcatatgc agcggcgctt tacccggttg cagcagcgac ataccctgtt cggaacgggt
     2461 cactaacaga gccgagagtt cgtaatcggc aatcagtttc atgccgcgct caacaatctc
     2521 ttcttcggtc ttacatttac cgacaacagc ttcaaattcc gagagattcg gtgtcagcag
     2581 cgtagcgccg cggtagcgct caaaatcggt accttttgga tcaatcagca ccggaacgcc
     2641 cgctttacgc gccagttgga tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc
     2701 agaaagcacc agcgcgccaa tcgaactcag cgcctgatta atccgctcgt gcagcggctg
     2761 cggatcaaca ccttcgaaac cttcttcaaa atccagacgg atcagctgtt ggttgcggga
     2821 aagtacccgt aatttggtga tggtcggatg cgtcggtaca gaaacgaagt cgcatttgac
     2881 gttgacgtcg gccagagatt tactcagcgc gcgcgctgca tcgtcaatgc ccgtcaaccc
     2941 gaccaggcgt gcattagcac cgagagaagc gatattcatc gccacgttag ccgcgccgcc
     3001 cggacgttct tcgatggtat tcactttaac cacgggcacc ggcgcttccg gcgagatacg
     3061 actggtgggg ccgtaccagt aacgatccag catcacatca ccaaccacca tcactcctgc
     3121 acgttcaaac tctggcagcg ttactttcat tcctgtctcc tgagagattc aaaatttgcg
     3181 cgcgataata ccatacttca ttcttccacc agccacttct gccagcttgc ccgcaccaga
     3241 tcacgctctg cggtgaagca atcctccgac acatggcccg gcaattcctg taatgccaga
     3301 tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca gcgccatcgc ttcctgctct
     3361 tccataatgt cgttttgcgc cagcagctcc agaatacgca cgttgtctga ccagcgcgtt
     3421 aacttcggtt tttcatgagc gtagcgcaac accagatatt gggtaataaa ttcgatatcg
     3481 gtaattccgc cttcatcggc tttgatatca aagcgattac gatgtttatt gccgagatga
     3541 gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca gagttttacc ttctcgcggc
     3601 agtgtcataa tctcacgacg caccgcgtca aagtgcgcag tgagctgcgg atcgccgtac
     3661 actacacgcg cacgcaccag cgcctgatgt tcccacgtcc aggcctcgtt tttctgataa
     3721 tcggcaaatg cttctgcgga tgtcaccagc attcccgccg ccccggacgg acgcagtcga
     3781 gcatccactt catacaaaat gccggaagag gtacgcgtac tgaacagatg cataatgcgt
     3841 tgcgccagac gcagataaaa ctgccgcccg tcgatttccc gctcgccgtc agtcatcgca
     3901 tccattgggc aatcatggag gaagataagg tcaagatcgg aactgtagcc taactcccag
     3961 ccgcccagct tgccgtagcc gaccaccgca aaaccgcgcc cttcacgttc gttgaggtga
     4021 tttggcttac cgtagcgggc caccatttga ccccacgcct gctgaacgac ggcatctatc
     4081 atagcttctg ccagccaggt taagtgatcg ctcactttca ttaccggtag cgtaccggcg
     4141 atatccgctg ccgcaatgcg taacagctgc gcctgtttga actgacgcag cgcctcaagc
     4201 tgttgttctt cgtcatcttc cggcacgcgc agcaaatact ggcgcagttc atcacggtag
     4261 gcatcggtcg ccgtcggctg ataaagggta tttggatcga gcaattcatc cagcaataat
     4321 ggataacgcg ctagctggct ggcaatcatc ggcgatgcgg cacacagaga aatcaaatgt
     4381 ttaagcgccg cagggaattc actgagcaat tctaaatagg ttgtgcgggt aacaatcccc
     4441 accagcaagg cggtaatgcg cgacagcgtg acagcagcgt cttcacgcgc acagacatca
     4501 cttagcagat gtggcatcag atggtcgagc acctgacgtc ctcgcggccc gatagtacgc
     4561 ttatccagct ctttgcggaa atcagcaatc atcgttagga cctgtttgcg atcatcctcg
     4621 ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca acgcatcctg ccacagctca
     4681 cgccactgtt ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca
     4741 ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcag ttgcggccag
     4801 tcagcaaagt ccatcgccca cgccagccgc gcacggttaa gctcatcaga aggaagcgtt
     4861 tgggtttgtt cgtcgttaat gctttgcagc aggttttcca gacgccgcag gaacagatat
     4921 gccactcgca attgttcagc atcgttttca gaaagcagat gcagcgcggc aatggcgctg
     4981 agcgttggca gtaaagagcg cgactgcagc aacggttcgc gcccaccgcg aatgagctgg
     5041 aacacctgaa cgataaattc aatttcgcga atgccgcctg cgccgagttt gatattgtcg
     5101 gtcaaaccac gtcgacgcac ttcacgggca atcatccctt tcatgttgcg cagcgactga
     5161 atcacgctga aatcgatgta acgacggaaa acaaacgggc gcagcatcgc acgcaactcg
     5221 ttagcataga cgccttcgct atcgcccata atccgcgctt tgaccatcgc gtaacgctcc
     5281 cagtcgcgtc cctgctcctg gtaataatcc tccaacgcgg caaagctcag caccagcggg
     5341 ccactttcgc caaacggacg cagtcgcata tccacgcgat agacgaagcc atcctgcgtt
     5401 ggttgatcca gcactttaat cagccgctgc cccatgcggg taaaaaactg cgcgttatcc
     5461 agttcccggc gtccgccctg cgtacaacca tgttccggcc aggcaaaaat cagatcgata
     5521 tcagaggaga aattcagttc cccgccgccc agcttaccca tgcctaagat cagcagcggt
     5581 tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc ggcagcaggc gtcatacagc
     5641 cagtcacgcg ccgcaacaat cagcgtctcc gccagataac tgagctgctg caatatgctc
     5701 tcttcagtaa ccagtgccag cgtttgcgcc catgcgatgc gcaccataat gcgccgccgg
     5761 aatagccgca gctcgcgcat taacccggct tcgtcgctca cattactgag cgcctcctgc
     5821 aaccacacgc tgtaatgttg ccattcgtca gcctgtggcg gttggctttc cagttccgtc
     5881 agccactctg gatgcgcaat cacgctgtcc tgcacaaaat cactgaaagt aagtactgac
     5941 ttcgcctgtg cactaagtga ttcctcggct aaaagctctg gcagccgctc aacaacggtc
     6001 tgccagtact gctgtaacgg tgaagagagc ggcttcataa gcgattttat ccttgcctga
     6061 ttaacgtttt ccgctgtgca accagaacgg ttcctgattg tttgcctcat tacggaaatg
     6121 ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc agcccctgcc agttctccag
     6181 ccaggcttgc gcgacgacag gatcatagta acccgccagc aacagtattg agtcaatatc
     6241 acgcatcagg cgtggcaact ggtcacggta gcgatcgcct aacggctggc agaaaacgct
     6301 tttcagttca gcggcatggc gggaaagatg gatatcggca aagcgtttga aggagtcgct
     6361 aattttgccc tgcgctttgg catctaaaaa cggctgccat gctttgctta ccagccattc
     6421 ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag accgccgtca cggcagaaac
     6481 cgcagaagcg atggtcgctt cgcattgagt cagcaaatca cgtaagtgag tactcgcttt
     6541 acgcggcaca atgccaccga acagcatcag tgtatgacgg actaggctaa tggctgccag
     6601 cacctgctct ttcgccgcat cgttgccgcg tacccacagt tcttcatgat actgccattg
     6661 cgctaacacc agctcgagcg ccgcttccag cccctgttcc acatcggctt ttgccgcaac
     6721 atgcaaaatg gtggtcggtt tgatttcacg cgccggattg ccctgcgcca gatgatagcc
     6781 acgcgccgct ttgctcaggc tgccctggcg taatccggtt tgcgatacca gttggttcgc
     6841 cagtttcagc accgcgcgcg tgtcgccgct aagcagctcc agttccagct cgcagatagg
     6901 ctcagcaaat tcacccgctt tcacttcccc ctggtcgaga gcgatttcaa ttcggctgcc
     6961 atcgatctct actagccatt tttcgcgata aaaatcggtg ctaaacagcg actgcacgcg
     7021 ggaagcgaga tcggcaggca attcgccgtt cggccagatt tccgtcggta actgcgccag
     7081 gtcgagcgtc ggttcgctca acgccacgtt atattccggg cgctggtgta agccgcctgt
     7141 cactcttcct gcaactttca tggtcatctc atagcgaccg ttttcgccac gaatacgtaa
     7201 gcccatatcg tgcctacgca gccagttatc cggcgtttcg tagtaaatat tcagcaactg
     7261 cacggggtca tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg cctcaacggc
     7321 actgtgatta acaataaact ttaattcgat ttcctgagcc atggccttta cttatcggtt
     7381 atgtcacata agtgacgatg aacggcgaac ttaatgcgat cttttttgtc agtagatagt
     7441 attttgcgcc aaattgccat gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg
     7501 gcaagacctg acacagagga ttccgattga tgtcgaatgc tttgcgtagc gacactgatg
     7561 ccactactat cgttccactt tccatgacaa taacgacagc ctgatgccaa aattacgcct
     7621 gatcggatta actttactcg cacttagcgc gactgccgtc tcacacgctg aagaaacgcg
     7681 ctatgtttct gacgaactga atacctgggt ccgtagcggt ccgggagatc attatcgcct
     7741 cgtgggcacg gttaacgccg gcgaggaagt gaccttatta caaactgacg ccaacaccaa
     7801 ttatgcccag gtgaaagaca gctctggccg taccgcctgg atcccgttga aacaacttag
     7861 cactgagcca agcctgcgct cccgtgtgcc agatctggaa aatcaggtca aaaccctgac
     7921 cgataaactc accaatatcg ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa
     7981 agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa
     8041 cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc gtacagctgg atgacaaaca
     8101 gcgcaccatc atcatgcaat ggtttatgta tggcggtggc gtgctggggc ttggcttgct
     8161 gctcggtctg gtactgccgc acctgatccc aagccgcaaa cgcaaagatc gctggatgaa
     8221 ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa ttgtgtattt gacgaaggga
     8281 tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg gatgcattgt taggactatc
     8341 ggtcaaagac agagattggg tggtggtcgg cagtacgcca caggagatgc tcgacgcggg
     8401 ctaccagcag gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc atgaagagta
     8461 tgcgctggca cgtaccgaac ggaaatccgg ttccggttac accggtttta cttgctatac
     8521 cgcaccggat gtcacgctgg aagatgatct taagcgtcgc gatctgacca ttaatgcgct
     8581 ggcccaggac gataacggcg agattatcga cccgtacaac ggtctgggcg atctgcaaaa
     8641 tcgtctgttg cgccatgttt cccccgcttt tggcgaagat ccgttacgcg tactgcgcgt
     8701 ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt attgccgatg aaactctgac
     8761 gctgatgcgc gagatgaccc atgcgggtga actggaacac ctgacgcctg aacgggtatg
     8821 gaaagagacg gaaagcgccc tcaccacccg caatccccag gtgttcttcc aggtactgcg
     8881 cgattgcggc gcacttcgcg ttttattccc ggaaattgac gcactgtttg gcgttccggc
     8941 acctgccagg tggcatccgg aaatcgacac gggtattcat accttaatga cgctctcaat
     9001 ggcggcgatg ctgagtccgc aggttgatgt ccgtttcgca actttatgcc acgatctcgg
     9061 taaaggactg acgccgccag aactttggcc gcgtcatcat gggcatggcc cggcgggtgt
     9121 taagttagtg gaacaactat gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc
     9181 cagactggtg gctgagtttc acgatctcat ccgcaccttc ccaatgctga acccaaaaac
     9241 catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa ccgcagcgta tcgagcaact
     9301 ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt tttgaatcag cggactaccc
     9361 gcaaggccgt tggttgcgcg aaggctggga agtggcgcag tcagtgccga caaaagcggt
     9421 cgttgaagcg ggatttaaag gcgtggagat tcgcgaggag ctgacccgac ggcggattgc
     9481 ggcggtagcc agctggaagg aacaacgttg cccaaagcct gactgagtat aaaactaaac
     9541 gttagcacga accgtcccct cgccccttcg gggagagggt tagggtgagg ggaacagacc
     9601 agcaccggtg cgaacaatca cccccacccc ggccctctcc ctacagagcg agggagaaaa
     9661 cccggcctgc tcccgaacct tttctcaatc aaaagaacac gacatacacc gcagccgcca
     9721 caataaagcg ataaatggcg aacgggataa acgaaatgcg cttaatcaat tgcaggaagg
     9781 ttttaatcgc tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga
     9841 tatcgccggt tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca
     9901 tcatcggtac cgccagcagg aacgagaact cggaagcagc gtaacggctc acgcccatca
     9961 gcatcccacc tgaaatggtc gccccggaac gggagaaacc cggccacagc gccagacact
    10021 ggaaacagcc aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg
    10081 gctctttcgg cttcaggcat tcggcggcaa tcagcaacaa accgccaacg accagcgcat
    10141 acatcacatt tatcgggtta aacaatgact taatcgtgtc gtggaataat agccccagta
    10201 ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt taaacgacct ttgctttcac
    10261 cttcgtgctg caacgggcgg ccaaagtgga tgccaatcag gccaaacaga cgccgccaga
    10321 acatcactac taccgccaga attgatccta actggatcac aacttcaaag gttttcgccg
    10381 tgtcgccctc aaaccccaac aagtgaccga caataatcat atggcccgtg ctggataccg
    10441 gcagaaattc tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca
    10501 tatcgctcat caataaaccc ctaaattatt aaaatgtacc gcttgtccga actactgcag
    10561 tatgaccagg ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat
    10621 tattgccacg ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc
    10681 tgagtttgat acgcacccac ggcgagttga agcgtgctaa cagcagctcc gccacctctt
    10741 cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac gtggctgacc accgtttctg
    10801 caatgtcagc gtaactgagg caatccgcca catcatcact tttcgccgct ttacggttat
    10861 cccatgccat ttcgatatcg aacactaact tctgttcgat ggtctgttcc cagtcgtaaa
    10921 caccaatagt ggtgattacc gaaagttgct ctataaatac aatatccatc acgtcctgcc
    10981 tgctttttgg ctaaccggat accacttccg gcgaaatgtg cgtattatcc acagattcat
    11041 cgttgaacac gaattttcaa aacggaacag cttatgagtg caatcgcgcc tggaatgatc
    11101 ctcatcgcgt acctctgcgg ctccatttcc agtgccattc tggtttgccg cttgtgtggg
    11161 ctgcccgatc cgcgaaccag cggctccggc aatccaggcg caaccaatgt gttacgtatc
    11221 ggtggcaagg gagcagccgt agcagtactg attttcgacg ttctgaaagg aatgttgcct
    11281 gtctggggcg cgtatgaatt aggtgtcagc cccttctggc taggcttaat tgccatcgcc
    11341 gcctgtcttg gacacatctg gcccgttttc ttcggtttta aaggaggaaa aggcgttgct
    11401 accgcttttg gagcaatcgc accaattagc tgggatctca ccggagtaat ggcgggaacc
    11461 tggttactga ccgtgctatt gagcggatac tcgtcgctgg gagcgattgt cagtgcactg
    11521 attgctccgt tttatgtctg gtggtttaag ccacaattca ccttcccggt ttcgatgctc
    11581 tcttgcctga tcctgctgcg tcatcatgac aacatccagc gtctgtggcg tcgtcaggag
    11641 acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg atttctggtg
    11701 gatctacatg acctgatagc cttcatcggg cttgcccagc cgttgctggc accatgccgc
    11761 cagaaattcc gcgcagacac gaatttcatg ctgcgataga gcagctcccg gtaaacagcc
    11821 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    11881 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    11941 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    12001 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    12061 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    12121 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    12181 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    12241 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    12301 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    12361 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    12421 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    12481 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt
    12541 tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    12601 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    12661 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    12721 ttcgcgttga ttcacgaact gttaattaca tattaacggt ggcaaaaaat ataaaaccac
    12781 atttttagtg ttagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    12841 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    12901 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    12961 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    13021 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    13081 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    13141 cgtggaagag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    13201 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    13261 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    13321 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    13381 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    13441 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attttgcgcc cgattggcag
    13501 ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag gactcaaccg
    13561 tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg catcgtcgcg
    13621 gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt
    13681 tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    13741 cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc accgccgttt
    13801 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    13861 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc cgacaaccag
    13921 tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa aactggtggt
    13981 tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca aggcgttaca
    14041 tgtgattttc ccagcaggct gcgcggtgct ggcggcaacc caggtagaag agattgaaga
    14101 agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt
    14161 cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa
    14221 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    14281 caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa cacgatgaaa
    14341 ccttccactg aatggtggcg atatcttgcg ccgctggcgg tcatcgccat tattgctcta
    14401 cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt ttttactggc
    14461 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc
    14521 tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct cgcccagcca
    14581 ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc taattcggtt
    14641 atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggcttgga
    14701 cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt tctcggctat
    14761 gcggtgatgt tctccgagct gatcctggca cctgtaacac cgtccaactc ggcgcgtggt
    14821 gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    14881 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc
    14941 gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat tggactgatg
    15001 aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat gttgccgctc
    15061 agcattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc gccggtactg
    15121 aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    15181 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc
    15241 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    15301 ctgcgcatta tcagttggga cgacattgtc agtaataaag cggcatggaa cgttttcttc
    15361 tggctggcct cgcttatcac cctcgcgacc ggactcaaca ataccggttt tattagctgg
    15421 tttggcaaac tgttagcagg cagcttaagt ggttattcgc cgacgatggt gatggtgacg
    15481 ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc gtatacctgc
    15541 gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    15601 ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    15661 ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta ctggcgactg
    15721 ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt actgtggatg
    15781 cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca cccaatagtt
    15841 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc cgagatccgc
    15901 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt
    15961 acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca tttcagccag
    16021 cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc gcttaaagcc
    16081 cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa
    16141 ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa tcgtgttcgc
    16201 cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    16261 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta acggtccgcc
    16321 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc
    16381 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    16441 accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt ccagcatcgg
    16501 cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc aggcaaacgc
    16561 cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag ggcctgcggt
    16621 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat
    16681 caacggtacg gttttacgca catgatcgcg ggaggccagt tcaggcacga cgccgccgta
    16741 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    16801 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    16861 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    16921 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    16981 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    17041 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    17101 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    17161 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    17221 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    17281 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    17341 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    17401 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    17461 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    17521 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    17581 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    17641 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    17701 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa
    17761 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    17821 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc
    17881 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    17941 atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac
    18001 gcagttacga tcgtttccgc gaacgggtga tgttccccat tcgcgataaa cgcggtcggg
    18061 tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg
    18121 aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    18181 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    18241 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    18301 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    18361 gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc
    18421 tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta
    18481 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    18541 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    18601 cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    18661 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    18721 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    18781 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    18841 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    18901 agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    18961 cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct
    19021 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    19081 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    19141 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    19201 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    19261 ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc
    19321 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    19381 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    19441 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    19501 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    19561 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    19621 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    19681 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    19741 ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    19801 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    19861 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    19921 agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga
    19981 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta
    20041 cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga
    20101 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    20161 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    20221 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    20281 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    20341 aaaactgcac gatgtctctg aagaagtgca ccgcgccctg caaaaactgc agcagattga
    20401 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    20461 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat
    20521 ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    20581 taacatcggc ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    20641 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    20701 caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg
    20761 ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct
    20821 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    20881 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    20941 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    21001 ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg cgtttcggta tcgatatgaa
    21061 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    21121 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg
    21181 tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag
    21241 tctctctgca caaacgccac cttt
//
LOCUS       DAJVQR010000069        21160 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVQR010000069 DAJVQR010000000
VERSION     DAJVQR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            20..793
                     /locus_tag="OQM52_002702"
                     /pseudo
     CDS             20..793
                     /locus_tag="OQM52_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="OQM52_002703"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="OQM52_002703"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1564499.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OQM52_002704"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OQM52_002704"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1564500.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OQM52_002705"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OQM52_002705"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1564501.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="OQM52_002706"
     CDS             complement(4991..7225)
                     /locus_tag="OQM52_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1564502.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OQM52_002707"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OQM52_002707"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1564503.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="OQM52_002708"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="OQM52_002708"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1564504.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OQM52_002709"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OQM52_002709"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1564505.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="OQM52_002710"
     CDS             complement(12980..14320)
                     /locus_tag="OQM52_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1564506.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OQM52_002711"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OQM52_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1564507.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="OQM52_002712"
     CDS             complement(16108..16557)
                     /locus_tag="OQM52_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1564508.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OQM52_002713"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OQM52_002713"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1564509.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="OQM52_002714"
     CDS             complement(17357..17686)
                     /locus_tag="OQM52_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1564510.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OQM52_002715"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OQM52_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1564511.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="OQM52_002716"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="OQM52_002716"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1564512.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..>21160
                     /gene="ppnN"
                     /locus_tag="OQM52_002717"
                     /gene_synonym="ygdH"
     CDS             19881..>21160
                     /gene="ppnN"
                     /locus_tag="OQM52_002717"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1564513.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRM"
BASE COUNT         5209 a         5639 c         5049 g         5263 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 tcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccatcata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc actggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga
//
LOCUS       DAJVQR010000070        20914 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVQR010000070 DAJVQR010000000
VERSION     DAJVQR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..484
                     /locus_tag="OQM52_002718"
     CDS             <1..484
                     /locus_tag="OQM52_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1564514.1"
                     /translation="KWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVE
                     NMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVEC
                     FFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV
                     "
     gene            586..738
                     /gene="hokA"
                     /locus_tag="OQM52_002719"
     CDS             586..738
                     /gene="hokA"
                     /locus_tag="OQM52_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1564515.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(925..1137)
                     /gene="cspA"
                     /locus_tag="OQM52_002720"
     CDS             complement(925..1137)
                     /gene="cspA"
                     /locus_tag="OQM52_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1564516.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1418..1708)
                     /locus_tag="OQM52_002721"
                     /pseudo
     CDS             complement(1418..1708)
                     /locus_tag="OQM52_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2142..2852
                     /locus_tag="OQM52_002722"
     CDS             2142..2852
                     /locus_tag="OQM52_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1564517.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2902..3876)
                     /gene="ghrB"
                     /locus_tag="OQM52_002723"
     CDS             complement(2902..3876)
                     /gene="ghrB"
                     /locus_tag="OQM52_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1564518.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3980..4630)
                     /locus_tag="OQM52_002724"
     CDS             complement(3980..4630)
                     /locus_tag="OQM52_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1564519.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4793..6991
                     /locus_tag="OQM52_002725"
     CDS             4793..6991
                     /locus_tag="OQM52_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1564520.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6960..7400)
                     /locus_tag="OQM52_002726"
     CDS             complement(6960..7400)
                     /locus_tag="OQM52_002726"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1564521.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7397..7960)
                     /gene="tag"
                     /locus_tag="OQM52_002727"
     CDS             complement(7397..7960)
                     /gene="tag"
                     /locus_tag="OQM52_002727"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1564522.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8118..8816
                     /locus_tag="OQM52_002728"
     CDS             8118..8816
                     /locus_tag="OQM52_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1564523.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9045..10253
                     /locus_tag="OQM52_002729"
     CDS             9045..10253
                     /locus_tag="OQM52_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564524.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10577..12268
                     /gene="eptB"
                     /locus_tag="OQM52_002730"
     CDS             10577..12268
                     /gene="eptB"
                     /locus_tag="OQM52_002730"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1564525.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12360..12436
                     /locus_tag="OQM52_002731"
     tRNA            12360..12436
                     /locus_tag="OQM52_002731"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12394..12396,aa:Pro,seq:cgg)
     gene            13180..14787
                     /gene="dppA"
                     /locus_tag="OQM52_002732"
     CDS             13180..14787
                     /gene="dppA"
                     /locus_tag="OQM52_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS1564526.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15095..16114
                     /gene="dppB"
                     /locus_tag="OQM52_002733"
     CDS             15095..16114
                     /gene="dppB"
                     /locus_tag="OQM52_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1564527.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16124..17026
                     /gene="dppC"
                     /locus_tag="OQM52_002734"
     CDS             16124..17026
                     /gene="dppC"
                     /locus_tag="OQM52_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1564528.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17037..18020
                     /gene="dppD"
                     /locus_tag="OQM52_002735"
     CDS             17037..18020
                     /gene="dppD"
                     /locus_tag="OQM52_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1564529.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18017..19021
                     /gene="dppF"
                     /locus_tag="OQM52_002736"
     CDS             18017..19021
                     /gene="dppF"
                     /locus_tag="OQM52_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1564530.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPEVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19051..20322)
                     /locus_tag="OQM52_002737"
     CDS             complement(19051..20322)
                     /locus_tag="OQM52_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1564531.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20798..20905
                     /locus_tag="OQM52_002738"
     CDS             20798..20905
                     /locus_tag="OQM52_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1564532.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         5051 a         5136 c         5484 g         5243 t
ORIGIN      
        1 gaagtgggtt accgatgtta ctgaatttgc agtcaatggg cgcaagctgt atttgtctcc
       61 agtaatagat ctcttcaaca acgaagttat ttcttacagc ctttcggaaa gaccagtgat
      121 gaacatggtt gagaatatgc tcgatcaggc attcaaaaag cttaatcctc acgagcatcc
      181 tgttctgcac tctgaccagg gatggcagta tcgtatgaga agatatcaaa atatccttaa
      241 agaacatggt attaaacaaa gcatgtccag aaaaggcaat tgtctggata atgctgtggt
      301 ggagtgtttc tttggaacct taaagtcgga gtgtttttat cttgatgagt tcagtaatat
      361 aagcgaactg aaggatgctg ttacggaata tattgaatac tataacagca gaagaattag
      421 cctgaaatta aaaggtctga ctccaattga atatcggaat cagacctata tgcctcgtgt
      481 ataactgtcc aactttttgg ggtcagtaca aagtgaggct accctatgcc tgaacaccat
      541 aaggttagcc tcttactcgt tggaaatcaa cacagggggc tgggaatgcc gcagaaatat
      601 agattacttt ctttaatagt gatttgtttc acgcttttat ttttcacctg gatgataaga
      661 gattcactgt gtgaattgca tattaaacag gagagttatg agctggcggc gtttttagcc
      721 tacaaattga aagagtaaga gtcttcgacg ggaaattatt cccgccttac ttacggcgtt
      781 gcgcattctc attgcaccca tatttattct tcacaaaaat aataatagat tttattacgc
      841 gatcgattat ttatttcctg aaaaaaaata aaaaaatccc cgccaaatgg cagggatctt
      901 agattctgtg cttttaagca gagattacag gctggttacg ttaccagctg ccgggccttt
      961 agcgccgctt tcgatggtga aggacacttt ctgaccttcg tccagagatt tgtaaccatc
     1021 gttctggata gcagagaagt gtacgaacac atctttagag ccatcgtcag gagtgatgaa
     1081 gccgaagcct ttgtcagcgt tgaaccattt tacgatacca gtcattttac cggacatagt
     1141 gtattacctt taataattaa gtgtgccttt cggcgatatg gcgtgcttta cagattttga
     1201 agcgttaaag gaatgtgcac tacgaggggt atcaacgata actcttgaag ggacttgcct
     1261 tactacactg ctttaatggt ctgtacgtca aaccgttgat gtgcattaag ccactcattg
     1321 gcgggtgatg caacaattat ttttcatatt tatgattaat cggttggaaa acggtcctgt
     1381 catcaggacc gtaaacagca ataaagtgga taaaagtcta tttcatcaac tgcttactta
     1441 atgccgggtt ggcttgaatc aaacgcatca attttagttc ggcacttgaa ggcttcacgc
     1501 gtctggattc ccattccttt accatggcga ctgatacgcc caaaacccgg gcgaaatcat
     1561 cgatttttaa tcctgtccct tttcataact gctcaatttc ggtacaggac gttgttctgt
     1621 gcgtcagggt aattttctgc gtttcatctt taaaaacaat ctgttccagg ctgctcaaca
     1681 gctcatgcat tggatcttta tattccattg aaaactcctc aaatcacact gcgggatcgt
     1741 gaattgcatc gaagctcatt aagaatagtc gggaaaagca gacacagcgg ggtgaagtca
     1801 gggcatgctc tggctatgat tcgattcagc ctgtcattta gcgtgactta tagccaccta
     1861 acgatctgat aaatgccgat atatctctta tcactcctgt tttgtaaaga tttcgcaaat
     1921 ctaaccttct gcgaaggcgt aaatctctgg ctacaaaaaa ggcaacggca ctatcatttt
     1981 ttaacaatga aacaacaaat taaccttgtt tcattttctc catgatttta ccaggaagat
     2041 tacgtagaaa gggtatcctg caagcctgtc ctggactatc cttgtcacgt cagacacgcg
     2101 tgtgtcgttg tgcgcttttt ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct
     2161 gctctcgctg gtttgcgcct cttgcggcgt tattaatggt agttagcctg agtgggtgtt
     2221 ttgataaaga aggcgatcag cgtaaagcgt ttatcgactt cctgcaaaat acagtgatgc
     2281 gtagcggtga acgtctacca accctgactg ccgatcagaa aaaacagttt ggtccttttg
     2341 tctctgatta cgcgattctg tatggttatt ctcagcaggt gaatcaggcg atggattccg
     2401 gtctgcgccc ggtcgtagac agcgttaacg caattcgcgt accgcaggat tacgttacgc
     2461 aaagcggtcc gctgcgtgaa atgaatggtt cgctgggtgt gctggcgcaa caactgcaaa
     2521 atgcgaagtt gcaggccgat gctgcgcact ctgcgctaaa acagagcgat gacctgaaac
     2581 cggtatttga tcaggcattc accaaagtgg taacgacgcc agctgatgca ttgcaaccgt
     2641 tgattccggc ggcacaaact ttcacgcaac aactggtgat ggttggagac tacattgctc
     2701 agcaaggtac tcaggtgagc tttgtcgcaa atggcatcca gttcccgact tcacagcagg
     2761 caagtgaata taacaaactg attgcgccat taccggcaca acatcaggcg tttaatcagg
     2821 cctggactac agcagttact gcaacccaat aaagagtaaa agcccgagcg attatcgggc
     2881 tttacttcac gcagtcgcag cttagtccgc gacgtgagga ttcacacagt tcttctcaac
     2941 ctttccttgt aacgcatcaa tcaaattatc cacggcacag gcggccatgc cataacgcgt
     3001 ctcatgggtg gcagatccaa tatgcggtac tgcgacgacg ttggccattg agagcaacgg
     3061 cgaatctacg gacagtggct cttgttcgaa gacatccagc ccggcggcgt gaatttcgcc
     3121 tttctgcaat gctgcgatca gtgcattttc gtcaaccacc gggccacgtc cggcattaat
     3181 gaaaatggcg gaggatttca ttttggcgaa ttgttctgcg ccaaacagat gatgcgtctc
     3241 atcagttaac ggcaggatca ggcaaacgaa atctgactct tgtaacagag tatccaaatc
     3301 gcagtagcgg gcgttgaagc gttcttctgc ttctttatgg tggcggcgcg cgttatagag
     3361 gatgggcatg ttgaagccaa agtgcacacg ttgtgccagc gccatgccga tccgtcccat
     3421 cccgacaatg cccagtgttt tatggtgaac gtcagtgccg taccagtccg ggcctatgct
     3481 cgcggtccat tcgcctgctt ttacccgttc tgctacctcc acaacccgac gagcggtaga
     3541 caacaccagc gccatcagcg tatcggcgac ggtttctgtt aatacggttg gcgtgtgcat
     3601 cagcagaatt tttcgggcgg taagcgcatc gacatcaaaa ttgtcatagc cgacggagat
     3661 cgttgatgtg gcacgcagtt tcggcatttt ttccagcaat gcggcatcta cattctcgtt
     3721 tgaacccagt aaaccttcag cttctgcaaa aattgctgca ttttgaacga cggtttgtgg
     3781 gctgaggttt gccacctggt gaacggtgaa atgcgcttgc aggcgttgca gtaaatcatc
     3841 aggtaaggct ttgtagagga taacggacgg cttcatgctt ctctccattc actgataagg
     3901 cgatcacctt agcctgaaat atgatgaagg aaaactgaag aatgccaggt gatttctgca
     3961 tcacctggca tgaaagggat tacagcgggc ttaaggtaat ttctacacgg cggttttgcg
     4021 ccttaccttc tgcggtgctg ttgctggcga ttgggttagc cgggccaagg ccctgagtac
     4081 ggatgcggct ggcgtccacg ccctgggtga tcaacgcgct ggcaacggaa tccgcacgct
     4141 gctgggagag acgcatgttc aggtcgtgac cccccgtgct gtcggtataa ccaatcacgt
     4201 taaccgccgt tttcggatac tctttcagca ccattgccac gccggtcagg gtgttagcgc
     4261 ctgccggttt caggggagcg ctgctgctgt cgaaggtcac attgttcggc atattgagga
     4321 taatgttatc cccgctgcgg gttacgctaa caccagtgcc gcgcattttg tcacgcagct
     4381 tcgcttcctg cacatccatg taataaccca cgccgccgcc cagagctgcg cctgctgctg
     4441 cgccaatcag cgcgccttta ccgcgatctt tcttcgaaga agagagcgca ccaatacccg
     4501 cgcccacgag agagcccaga cctgcgccga tagcagattt gcctgcttcg cgttcgccgg
     4561 tgtaagggtt agttgtgcag ccagataccg ccagagcgcc actcactacg gcggcaagta
     4621 agataaacac gtttcttcat tgttaatcct taataacctt tttattcttt gtcacgggtt
     4681 ccgtggcggg agattatgcc gcgtgaacat gaagattatt cctgggaata ctcggaaatt
     4741 tgtaagtaat atttaactgc tcaatacatc taacctttca ggagtcttcg gtttggccaa
     4801 ctcatcctca cgatattccg ttctgactgc cgcccactgg gggcccatgc tggttgaaac
     4861 cgacggcgaa accgtgttta gctcgcgtgg cgcgttagcc acaggaatgg aaaactcctt
     4921 gcagagcgcg gttcgcgacc aggttcacag caatacgcgg gtacgatttc caatggtgcg
     4981 caaaggcttt ctcgcgtcac cggaaaaccc gcaaggcatt cgtgggcagg atgaatttgt
     5041 tcgcgtgagt tgggatgagg cgctggcagg cggttatacc gggcatctgg gggattattc
     5101 gaccggtgcg gcacaggcga tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca
     5161 gcaaaccagt tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa
     5221 cccactcaat acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt
     5281 ttccgcactg cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac
     5341 cgtcgatttc tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc
     5401 gctgatgctg gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct
     5461 ggcgcgttgc accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg
     5521 aatagcgaaa aacgccgaat gggcggcaga gatttgtggt gttggcgcag cgaaaatccg
     5581 cgagctggcg gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca
     5641 gcgccaacag tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg
     5701 gcaaatcggc acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtga
     5761 ccccacgcgg cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga
     5821 tgcggtggat aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc
     5881 atatcaacac aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg
     5941 cggtgccaac tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc
     6001 ggagctggtg gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatattgt
     6061 tctgcctgcg actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa
     6121 tcagcatctg gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt
     6181 tgatgttttt gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga
     6241 aggaaaaagt cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc
     6301 aagccagcag gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga
     6361 gatgccggaa aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc
     6421 gctggcgcat ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc
     6481 cgattacggt tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca
     6541 gggcaatgcc gaaccagaac agttgcaggt actttctgct catccggcgc accgcctgca
     6601 cagccagctg aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt
     6661 caccattcat cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt
     6721 gtggaactcc cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg
     6781 cgtgatttgc attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg
     6841 taaaaacggc gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg
     6901 ctgtgcgggt aatacgacgc tggcatggct ggaaaaatac aacggtccgg aactgacact
     6961 tacagcgttt gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc
     7021 catgcgcaat cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt
     7081 ttttgataaa cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc
     7141 ttaccaatac cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct
     7201 tccataatgc tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg
     7261 ttggcaagat aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata
     7321 aagggatgcc cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa
     7381 cgttgcgctt cccgaatcat ggtttatttc ccagatagca acagcagcca accacatgat
     7441 cattcaccag cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa
     7501 aaccacgttt tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt
     7561 cgctcaacgt tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt
     7621 cgggaaacgg ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg
     7681 cctgaatttt ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct
     7741 cttcctgcat tgctgcgacc ttcaccggat cgaactgatg aaagtaggcg cgatagtttt
     7801 cgcgtttttt gaggacggtg atccacgata acccagcctg ctgcccttca aagcagatca
     7861 tttcgaacag ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa
     7921 tataaagcgg gccctgactc acccagccgc aacgttccat actttccctc gctatgtacc
     7981 cgatcgtaaa aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata
     8041 cagggctact atgcccgata tccttcatac aatgacaata atattgccga attcggctct
     8101 tccctggagt cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc
     8161 tggcgagcgt ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat
     8221 atatcgttga tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc
     8281 aacctgatta caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac
     8341 tggaagtcaa tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct
     8401 ggaattttcc tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg
     8461 gcacaaccac ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt
     8521 tatggcatgc cagcgtgagt agcttaggct ggcgtgttga ctcccggctt ggcgatctcc
     8581 gaccctgggc gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat
     8641 caggcctgag ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag
     8701 gcgctgatat gttgctcaat caaaatattg ccgcctatgc tgcgctaact caggcagaaa
     8761 ataccactaa taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta
     8821 acagtcacaa ttgaaaccat tgaataacaa cagttgtggc gatagtgggt gctaacttac
     8881 caaataataa atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat
     8941 tccgttctga tggcatttca tgccgttttt ccccaagcat aaaatgcact tcgttatggt
     9001 tgtcggcaga gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt
     9061 atcagcgtac ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt
     9121 cggtttatac ccggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa
     9181 gccaggtcgc tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg
     9241 cgggcaaatt gcaggaacgt tttggcgtta aacgcgtcac cgtggcttcc ggcattttgc
     9301 tgggattagg cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg
     9361 ccggtgtgct ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg
     9421 tgaagtggtt cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc
     9481 tgggtagcct gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa
     9541 aaacctttgt gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa
     9601 tgaaagacgc accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca
     9661 cgctggcaga gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg
     9721 cctgcatgag cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac
     9781 accttgatgt ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag
     9841 gtcgtctggt gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg
     9901 gtcaggtgat ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga
     9961 cgttctttgc agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc
    10021 catcactggt cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt
    10081 acctcggttt cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt
    10141 tctatgtgac cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga
    10201 cgattcgtca gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag
    10261 ccgcgagcta ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac
    10321 aatgactcgt aaaatttggg ccagttaact ggcccttttt actgcttttt gtaacgctaa
    10381 agtctctttt caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca
    10441 ctttttcgct ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc
    10501 tccgcacttt tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg
    10561 tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt
    10621 gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag
    10681 ctatgcgcac gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac
    10741 cgtactggtg accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg
    10801 tattctggcg tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct
    10861 taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc
    10921 aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct
    10981 tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg
    11041 gcagcgtatt cgcagcctgg tggtcgtcgt actggcgggt attatggttt gggcaccgat
    11101 tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag
    11161 ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct
    11221 gtatgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa
    11281 gaagtttaca taccaggctc cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg
    11341 tgaaaccacg cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa
    11401 actggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa
    11461 actctcactg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac
    11521 attaaaagaa cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta
    11581 cgctatgcag agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg
    11641 tgagcagatt ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt
    11701 agacgaaatg cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca
    11761 taccaaaggt tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa
    11821 gccggaatgt attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga
    11881 caactcggtg acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa
    11941 gaaagcgatt gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct
    12001 gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg
    12061 gatgtcagat aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa
    12121 agaagccgac atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg
    12181 tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc
    12241 gcagacaaaa gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg
    12301 cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc
    12361 ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat
    12421 cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggtttttt tgcatttgta
    12481 gctcatgagg atgagaacct ccgggggcag ggaggctcga ctcgacgtag gcctgataag
    12541 acgcgccagc gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc
    12601 cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata tctcactatc
    12661 cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttg
    12721 cgttacttat ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat
    12781 cgctcagata tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc
    12841 aaatgttgct aaatattaag ctgattgttc tggttatgtt ggagtagcac aaatttctct
    12901 gctgcaaata gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt
    12961 aaatcccggt tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg
    13021 ggcattttat ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct
    13081 cataacgttg acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca
    13141 aagggttaaa acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag
    13201 tcagggatgc tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag
    13261 gctaaaactc tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt
    13321 acctccggca ccacctatga cgcctcttcc gtcccgcttt ataaccgcct ggttgaattt
    13381 aaaatcggca ccaccgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac
    13441 ggtaaaacct ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa taaagaattc
    13501 aaaccgacgc gcgaaatgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac
    13561 gcgcaaaacc cgtaccataa agtttctggc ggcagctacg aatacttcga aggtatgggc
    13621 ttgccggagc tgatcagcga agtgaaaaag gtggacgata acaccgttca gtttgtgctg
    13681 actcgaccgg aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc tattctgtca
    13741 aaagaatatg ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca
    13801 atcggaaccg gtccgttcca gttacagcag taccaaaaag attcccgtat ccgctacaaa
    13861 gcgtttgatg gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc
    13921 cctgatgctt ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac
    13981 ccgaacccgg cagacatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg
    14041 ccggggctga acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg
    14101 aaagttcgcc aagcactaac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat
    14161 cagggcgcgg gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac
    14221 gacgttcagg attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt
    14281 ctggaaaaag gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg
    14341 aacgctcgcc gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc
    14401 aaaatcgtca cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag
    14461 acggtaatga tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg
    14521 ttcagctgcg ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt
    14581 gaagatctga ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac
    14641 aagcaagcgc aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc
    14701 gtgtttgaac cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt aggcaaacat
    14761 cacttcgaaa acgtctctat cgaataatta aaagccatac aagactgatg gcaaaggcaa
    14821 aactgcctga tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta ttgaatttgc
    14881 acgattttgt aggccggata aggcgttaac gccgcatccg gcataaacaa agcgcacttt
    14941 gtcaacaatc tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa
    15001 tacttcattc tctgtccgac aagggcggga attgatttgt gagcaataca gacacgcagt
    15061 tccaggctgc gagtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt
    15121 gggactcgtc atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat
    15181 gatcccgggc gatccggtga tgatcatggc aggcgaacgt gggatctccc ctgagcgtca
    15241 cgtgcagctg ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta
    15301 catttggggc gtaatgcacg gcgatctagg catttcaatg aaaagccgaa ttccagtatg
    15361 ggaagagttc gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg cgatgatttt
    15421 tgctacggca gtcggtattc cggttggtgt gctggctgcg gttaaacgcg gttccatttt
    15481 cgatcacact gcagttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg
    15541 catgatgctg atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt
    15601 gagcgatatg gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac
    15661 cgccatctgg ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcccgc
    15721 cattgtgctg ggtactattc cgctagcggt cattgtgcgt atgacacgct cctcgatgct
    15781 ggaagtgctg ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg
    15841 ggtgattatc gtccatgcgc tgcgtaacgc gatgctgccg gtagtgaccg ttatcggcct
    15901 ccaggtggga acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg
    15961 tctggggcgc tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg
    16021 cgtattgctg gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg
    16081 cgtggtgaac ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga
    16141 aaataaagtg attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt
    16201 taaacgcaac aaaggcgcgg tcgtcgggct ggtttacgtc gccatcgtgc tgttcatcgc
    16261 gatctttgcc aactggattg cgccttataa cccggcggat cagttccgcg atgcgctgct
    16321 cgccccgcca gcctggcagg aaggcggcag tatggcacac ttgctgggca ccgatgacgt
    16381 cggtcgtgat gtgctgtcgc ggctgatgta cggcgcgcgt ttatcgctgc tggttggctg
    16441 tctggtggtg gtgttgtcgc tgattatggg cgttattctc ggcctgatcg ccggttactt
    16501 cggcggcctg gttgataaca ttatcatgcg cgtggtcgat atcatgctgg cgctgccaag
    16561 tctgctgttg gcactggtgc tggtggcgat tttcggcccg tcgattggta acgccgcgct
    16621 ggcgctgacc ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt
    16681 ggaggtgaat cgcgattacg tcaccgcctc tcgcgtggca ggtgccgggg cgatgcgcca
    16741 gatgtttatt aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg
    16801 tttctctaac gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc
    16861 gccaacgcct gagtggggca ccatgctctc cgacgtgttg cagttcgcgc aaagcgcctg
    16921 gtgggtcgtg accttccctg gtctggcgat cctgttgacg gtgctggcat ttaacctgat
    16981 gggtgacggt ctgcgtgacg cgctcgatcc caaactaaag cagtaagagg ttcgagatgg
    17041 cgttattaaa tgtagataaa ttatcggtgc atttcggcga cgaaagcgca ccgttccgcg
    17101 ccgtagaccg catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt
    17161 ccggctccgg taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc
    17221 gcgtaatggc agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag
    17281 agcgccgcaa cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc
    17341 ttaacccgtg ctacaccgtg ggtttccaga ttatggaagc gattaaagtg catcagggcg
    17401 gcaacaaaag tacccgccgt cagcgggcga ttgacctgct gaatcaggtc ggtattcccg
    17461 atccggcttc gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg
    17521 tgatgatcgc catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca
    17581 ccgcgctgga cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga
    17641 aagagaacat ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagctgcgc
    17701 ataaaatcat tgtgatgtat gcaggtcagg tagtagaaac gggcgatgca cacgccatct
    17761 tccatgcgcc gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc
    17821 aggacaaaga acgtctggcg tcgttgccag gtgttgttcc cggcaagtat gaccgcccga
    17881 acggctgcct gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac
    17941 cggcgctgaa tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg
    18001 ccgggaggcc cacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc
    18061 tatcgacctg aaaaaacatt atccggtgaa gaaaggcatg ttcgtgccgg aacgtctggt
    18121 gaaggcgctg gacggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg
    18181 cgaatctggc tgcggtaaat cgaccctcgg tcggttgctg acgatgatcg aaacgcccac
    18241 cggcggcgag ctgtattatc aggggcagga tctgctcaag cacgatccgc aggcgcagaa
    18301 gctgcgtcgg cagaaaatcc agatcgtctt ccagaatccc tacggttcgc tgaatccgcg
    18361 taagaaagtt gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga
    18421 acagcgtcgg gaaaaagccc tgtcgatgat ggcaaaagtc ggcctgaaaa ccgagcacta
    18481 tgaccgctat ccgcacatgt tttccggcgg acaacgtcag cgtatcgcta ttgcccgtgg
    18541 tctgatgctc gacccggagg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc
    18601 agtgcgtgcg caggtgctga atctgatgat ggatttgcag caggagttgg ggctttctta
    18661 tgtctttatc tcccacgacc tgtcggttgt ggagcacatt gctgatgaag tgatggtgat
    18721 gtacctaggc cgctgcgtgg agaaaggaac gaaggaccaa atcttcaata acccgcgcca
    18781 tccgtacacc caggcgctgc tctccgcgac gccacgcctg aacccggacg atcgtcgtga
    18841 acgcatcaaa ctcaccggtg aactgccaag cccgctgaat cctccgccgg gttgcgcctt
    18901 caacgcccgc tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga
    18961 ctacggaggt caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta
    19021 actcacaaaa ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa
    19081 gcatagcaaa atgccgtaaa gcagaatcag ccaggcttta aatccgcgaa gtttttccag
    19141 ttgtgcgact ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg
    19201 gcttcccaac tgaaagaaca ccagcactga tacacgaaac gaaacccaaa tcgtcaacgt
    19261 aatgacgata aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc
    19321 aataattcgg cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata
    19381 aatgccaaag aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat
    19441 gatatgtcca gggatcacct gcgcggccag tgccagtgcc gagatatttt gttcaaaggc
    19501 agaaacggct tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac
    19561 cgcgatgagc gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac
    19621 tttatctgct tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac
    19681 cgcagaaaag aagcaaaatg gaatggtaag caggacatcg cggaaaaaga cggaggcttg
    19741 cgggaaggcg gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac
    19801 aataatccct actttgacca ccaccattgg cccagaaatc ttaaacagta atcgttcacc
    19861 accagacgca atcgctatca gtacggcgaa aatagcgact ttataaagta gagattgtga
    19921 aagatcggca tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga
    19981 gagagagtag ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat
    20041 tccccagttc ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct
    20101 ttcagaaagg gtttttaaat aaatgtcctg cactacccag gtgacaggat aagcaatgat
    20161 cgctgcggta ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt
    20221 tccggcacca atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa
    20281 cggcaatttc tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag
    20341 aagtcggcct cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg
    20401 aatacgcagt atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca
    20461 attctacaaa attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac
    20521 tattgcaaat aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc
    20581 tgacagtaaa aagcaatcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa
    20641 tgttgcgggg gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt
    20701 tgcaggtatg tacctgacaa cataacgggg gctaatcttg aacctagacc actcaagaat
    20761 agccgcgaaa cgttgtcatt acaacacagg cggctatatg acgctcgcag agctgggcat
    20821 ggccttctgg catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt
    20881 gaactggctg aacaagcgga agtagcgtgt catg
//
LOCUS       DAJVQR010000071        20779 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVQR010000071 DAJVQR010000000
VERSION     DAJVQR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..579
                     /locus_tag="OQM52_002739"
     CDS             <1..579
                     /locus_tag="OQM52_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1564533.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OQM52_002740"
     CDS             597..1235
                     /locus_tag="OQM52_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1564534.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OQM52_002741"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OQM52_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1564535.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OQM52_002742"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OQM52_002742"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1564536.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OQM52_002743"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OQM52_002743"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1564537.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OQM52_002744"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OQM52_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1564538.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OQM52_002745"
     CDS             complement(5552..6613)
                     /locus_tag="OQM52_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1564539.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OQM52_002746"
     CDS             complement(6610..8007)
                     /locus_tag="OQM52_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1564540.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OQM52_002747"
     CDS             8163..9161
                     /locus_tag="OQM52_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1564541.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OQM52_002748"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OQM52_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OQM52_002749"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OQM52_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OQM52_002750"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OQM52_002750"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS1564542.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OQM52_002751"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OQM52_002751"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS1564543.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OQM52_002752"
     CDS             complement(14232..14855)
                     /locus_tag="OQM52_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1564544.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OQM52_002753"
     CDS             complement(14852..15274)
                     /locus_tag="OQM52_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1564545.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OQM52_002754"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OQM52_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OQM52_002755"
     CDS             complement(16089..17141)
                     /locus_tag="OQM52_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS1564546.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OQM52_002756"
     CDS             complement(17172..18014)
                     /locus_tag="OQM52_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCS1564547.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OQM52_002757"
     CDS             complement(18001..18882)
                     /locus_tag="OQM52_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS1564548.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OQM52_002758"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OQM52_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="OQM52_002759"
     CDS             complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="OQM52_002759"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCS1564549.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5668 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacacca gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DAJVQR010000072        20698 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVQR010000072 DAJVQR010000000
VERSION     DAJVQR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OQM52_002760"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OQM52_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1564550.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OQM52_002761"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OQM52_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1564551.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OQM52_002762"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OQM52_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1564552.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OQM52_002763"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OQM52_002763"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1564553.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OQM52_002764"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OQM52_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1564554.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OQM52_002765"
     CDS             complement(8217..9110)
                     /locus_tag="OQM52_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1564555.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OQM52_002766"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OQM52_002766"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1564556.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OQM52_002767"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OQM52_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1564557.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OQM52_002768"
     CDS             complement(10241..11473)
                     /locus_tag="OQM52_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1564558.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OQM52_002769"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OQM52_002769"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1564559.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OQM52_002770"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OQM52_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1564560.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OQM52_002771"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OQM52_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OQM52_002772"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OQM52_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1564561.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OQM52_002773"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OQM52_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1564562.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OQM52_002774"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OQM52_002774"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1564563.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OQM52_002775"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OQM52_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1564564.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4978 a         5722 c         5026 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccatta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaa
//
LOCUS       DAJVQR010000073        20689 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVQR010000073 DAJVQR010000000
VERSION     DAJVQR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            83..352
                     /locus_tag="OQM52_002776"
     CDS             83..352
                     /locus_tag="OQM52_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1564565.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            346..711
                     /locus_tag="OQM52_002777"
     CDS             346..711
                     /locus_tag="OQM52_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1564566.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OQM52_002778"
     CDS             complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OQM52_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1564567.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1538..2221
                     /gene="gntX"
                     /locus_tag="OQM52_002779"
     CDS             1538..2221
                     /gene="gntX"
                     /locus_tag="OQM52_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1564568.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2280..2855
                     /gene="nfuA"
                     /locus_tag="OQM52_002780"
     CDS             2280..2855
                     /gene="nfuA"
                     /locus_tag="OQM52_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1564569.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3216..4532
                     /gene="gntT"
                     /locus_tag="OQM52_002781"
     CDS             3216..4532
                     /gene="gntT"
                     /locus_tag="OQM52_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1564570.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OQM52_002782"
     CDS             complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OQM52_002782"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1564571.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OQM52_002783"
     CDS             complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OQM52_002783"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1564572.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9676..12381
                     /gene="malT"
                     /locus_tag="OQM52_002784"
                     /pseudo
     CDS             9676..12381
                     /gene="malT"
                     /locus_tag="OQM52_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="OQM52_002785"
     CDS             complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="OQM52_002785"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1564573.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="OQM52_002786"
     CDS             complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="OQM52_002786"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1564574.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14851..16449
                     /gene="rtcR"
                     /locus_tag="OQM52_002787"
     CDS             14851..16449
                     /gene="rtcR"
                     /locus_tag="OQM52_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1564575.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16431..17189)
                     /locus_tag="OQM52_002788"
     CDS             complement(16431..17189)
                     /locus_tag="OQM52_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1564576.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="OQM52_002789"
     CDS             complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="OQM52_002789"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1564577.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="OQM52_002790"
     CDS             complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="OQM52_002790"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1564578.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18597..20102
                     /gene="glpD"
                     /locus_tag="OQM52_002791"
     CDS             18597..20102
                     /gene="glpD"
                     /locus_tag="OQM52_002791"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1564579.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20371..20601)
                     /locus_tag="OQM52_002792"
                     /pseudo
     CDS             complement(20371..20601)
                     /locus_tag="OQM52_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4890 a         5693 c         5216 g         4890 t
ORIGIN      
        1 gagtcagtag atactctacc ctgcttaagt gctactttgt gctatcttgc atgtaacaca
       61 aagtagcact tagggaactg atatgcagac agtgaaatta cggcaacaag gaggagccat
      121 gatcgttact atcccacgag atcttgccat cgatcttggc tggtcaccgg gcacagagct
      181 taccgtggag aaaaaaggcg atagcgttaa tttgcgcgcc acagagcaca aaccgcgtgg
      241 gcgtcttacc gtcgcgcagt tgttgagcca aatcgatgaa agcgaaatta cggagctaaa
      301 tcagtccaca gaaggatggg ctgaagggaa aaaaggtaat gaagcatggt aaaggcacgg
      361 acgccacatc gtggtgagat ctggtatttt aaccctgatc cggttgccgg gcatgaactt
      421 caggggccac attattgcat tgtggtaacg gacaaaaaac tcaacaatgt tttaaaagtt
      481 gctatgtgct gcccgatttc aacaggggca aatgcagcac gttccacagg ggtgacggtg
      541 aacgtcctcc cccgtgatac gcaaaccggt aacctgcatg gcgttgtact ttgtcaccag
      601 ctaaaagccg tcgatcttat tgcccgtggc gctaaatttc ataccgttgc cgatgaaaaa
      661 ttgattagtg aagttatcag taaactggtg aatttaatcg acccacaata attagctctc
      721 acttaccacc aacaccctct gcttcaacgc caccagcagg tgacaaaact cgaccggatg
      781 cgaaataaac ggcgcatggg ccgctttggc gaagatatat gattcgctgt gaggccaaag
      841 tttatccagc atcggcacca ctttgcgcgg caccagaccg tcgagatagc catacaatcg
      901 caaaaacggc atggacacgt tttgcagcgg ctgacggaga tcgaccgttt tcaggatttc
      961 cagcccgcca ttaagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa
     1021 cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt tgtaacgcca ggaaccgctc
     1081 cactgtacgc tgaaaatcat cactgagttg ctgctgaaat cccgccagca cgtccggttt
     1141 tatccccggc cactcgtcac gagcactaaa acaaggtgac gacgccacgg tgaccagcgc
     1201 ctgaacacgc tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca
     1261 gcctaaccaa atggctttat caggtgcctg ttgcagcacg gcttcggcca tatcagcaag
     1321 tgacagcgca ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa ggtgcagcgt
     1381 aaaatgcgag ctaagttcct cgtcaatgca acgccacact tcggcattca gtccccatcc
     1441 gtgcagcagc acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat
     1501 ccgctattgt tctcttttga cttacaagga tgaacatatg ctaacagtac cgggattatg
     1561 ctggctatgc cgaatgccac tggcgttagg tcattggggg atttgttcgg tctgttcacg
     1621 cgccacccgc acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactccca
     1681 tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg tcacggttgc
     1741 cgactatgcg ccgccgttaa gtccgcttat ccaccagctt aaattttccc ggcgcagtga
     1801 aatcgccagc gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac
     1861 cgggttgcaa ttgccggatc gcatcatcag cgttccgtta tggcagcggc gtcactggcg
     1921 tcggggattt aatcagagcg atttgctgtg tcagccgtta tcacgctggt tgcactgcca
     1981 atgggatagc gaagccgtca cacgtacacg ggccactgcg acccagcatt ttctcagtgc
     2041 ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt gaattgcccg tgcaaggtcg
     2101 ccatatggtg attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca
     2161 gttgctttta cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta
     2221 gagcctcgat gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta
     2281 tgatccgtat ttccgatgct gcacaagcgc actttgccaa actgctggca aatcaggaag
     2341 aagggacaca aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggtg
     2401 tttcttattg tccgccggat gccgtggaag ccaccgacac tgccctgaaa tttgacctgc
     2461 tgaccgcgta tgttgatgag ttaagcgcac catacctgga agatgcagag atcgactttg
     2521 ttaccgacca gttgggttct cagttaacgc tgaaagcccc gaacgccaaa atgcgtaaag
     2581 tggcagacga tgcaccgctg atggagcgcg tggagtatat gctgcagtcg cagatcaacc
     2641 cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa gacggttacg
     2701 ccattctgca atttggcggc ggctgtaacg gttgttccat ggtcgatgtg acgctgaaag
     2761 aagggatcga gaagcagctg ctgaacgaat tcccggagct gaaaggtgtg cgcgatctca
     2821 ccgaacacca gcgcggcgaa cactcctact actaagttat cccctctttt gtagattgcc
     2881 cgatgcgacg ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga cgttggcaca
     2941 gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat aatatgacca acctctcata
     3001 atttaaattt accccgctct ggtgattctc aaacgccaaa tgttacccgt atcattcaca
     3061 tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg ggtatcagtc
     3121 gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt cagaaaattg
     3181 acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt gttgctatcg
     3241 gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc atcgctctcg
     3301 tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa gttattggct
     3361 ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg ggttttggcg
     3421 caatgctggg caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc accacgctga
     3481 ttgccaaatt tggtaaaaag cacatccagt gggcggtggt gttaaccggt tttaccgttg
     3541 gttttgccct gttctatgaa gtgggcttcg tgctgatgct gccgctggtg tttaccatcg
     3601 cggcttccgc gaatattcca ttgctgtatg ttggtgtacc aatggcggct gcactgtctg
     3661 tgacccacgg cttcctgcca ccgcatccgg gcccgactgc gattgccacc attttcaatg
     3721 ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc gtgattctcg
     3781 ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaatcccg gaaggtctct
     3841 acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc gtctggacct
     3901 ctctggtgcc ggttgtgctg atggcgatgc gtgcgattgc cgaaatgatc ctgccgaaag
     3961 gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggca acgctgattg
     4021 ccgtgctgat tgcgatgttc acctttggtc tgaaccgtgg tcgttcaatg gatcagatta
     4081 acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc atcggtggtg
     4141 gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt gcttccatga
     4201 tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc gtactgcgta
     4261 tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg gcaccgctga
     4321 ttgcaacgac gggtgttagc ccagagctga tggttattgc ggtgggttcc ggtagtgtga
     4381 ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt aacctgacta
     4441 tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg tgcggtctgg
     4501 taggctgtct gctgctgaat atggtgattt gataacactg actgccggat gcatcgcatc
     4561 cgacatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct
     4621 tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg gaaagcttgc
     4681 gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca
     4741 gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca
     4801 aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg
     4861 gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct
     4921 gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta
     4981 gatccccgct ctcccagtaa ccacgcagcg ttggcaggtc atgtgtcgcc gcaaccgcca
     5041 tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg ttttcgaaat
     5101 agagcacttt gtaagagtac acaccgctgc tgcgcagctt accaacaatc tctaccggta
     5161 cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa
     5221 tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat
     5281 acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt
     5341 tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg gtgatgatat
     5401 gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata tccggcggcg
     5461 cgccaaccga tgctttcagg caatatagtt cacggtcaca ccaggtttcc gccccacctt
     5521 ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta
     5581 tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga taaaaatcga
     5641 cgtcatcacg atgttcttcg cagaactgac gcacttctgg tgaatccacg ttcttataca
     5701 tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta
     5761 gcgcatcaaa ggcagcctgc cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa
     5821 acgcggccat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg
     5881 tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcgcgc gcctgttgca
     5941 gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat
     6001 cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa gacgggctgt
     6061 atgggctggc gctctccgga tttgccggat agagcgcatg aatcgggttc aggccaatga
     6121 acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa
     6181 taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc cacagctttt
     6241 gtttattcag caaagcctgc ggttcgtaac aacgtttcgg ggcgacaatc acccggcaat
     6301 gcgcgcgctg gtcgtcctgg gtgagtgtta gcgtgtgata accttccggc agcttcgtcg
     6361 gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgcgttcct tcttcggtgg
     6421 tcagctgcca gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg
     6481 tataaaccat gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca
     6541 tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat
     6601 tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca
     6661 tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat
     6721 ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct
     6781 ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg aaatccgcca
     6841 tcaccagata cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat
     6901 gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct
     6961 tatctttctt ccgccatttc accgggtcgt agcctttggc cagaatagcc ttcacttgtt
     7021 ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt
     7081 tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg
     7141 taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca
     7201 gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa
     7261 ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata atattcttcg
     7321 ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag
     7381 cctgcaggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct
     7441 ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct
     7501 caataccggt acgaactttc acaaactccg ccagacagac tttattcgcc tgcttgatct
     7561 cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat
     7621 cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc agtgcagggt
     7681 tgcactgttt gatccagcga cgtggggtaa taccgttggt aacgttatgg aatttgttcg
     7741 gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg
     7801 caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttat
     7861 cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacca
     7921 gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc agtaagcctt
     7981 tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag
     8041 cgaaagtttt gctggtgatg gcccaggcgt catcccagct catctggtgc tcatcggtca
     8101 gcacgcgcag cagttctgga atcgcgatag ttggatgggt atcgttcagc tgaataactt
     8161 cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc aaaatatccg
     8221 ctaccgaaca ggcacactgg aagtattgct gcatcaggcg cagcttttta ccggcagtat
     8281 ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgcct tgctgttcgg
     8341 cgcgcaggaa atcaccgtcg ttaaatttag ttagatcaaa cggatgcgca tgcgtcgcct
     8401 gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg
     8461 cttgaccggt aatggtaaac tccggctccc agcgtccgtc tttcatcact ttaccgccaa
     8521 tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc
     8581 agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac aaaccatatt
     8641 gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag tcgaggaagc
     8701 acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct tcttccagca
     8761 ggtcagtcag gttgatgtca taagccttca acgaatcctg tacatcctga taccagccga
     8821 gattcaacag gttgttgccc gtcagacgac caatcaaaaa ctccattgag atgtagttaa
     8881 catgtcgctg attcgccacc ggtttggcga atggctgagc acgcagcatt tcggccagtg
     8941 cttcactcac tgccagccac cactggcgag gagtcatttc agccgcagaa tttaagccat
     9001 aacgctgcca ctgacgtgaa agcacttcct gaaattgctt atcgttaaaa ataggttgtg
     9061 acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct
     9121 cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca
     9181 aggaaatgtg atctcgacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa
     9241 tgccatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa
     9301 aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata
     9361 tccagtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt agatggcact
     9421 ttcatcagga atgaagaaga aacccttgct taaatgaatc tgatgaacat aagggaaacc
     9481 agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaagatt tggaattgtg
     9541 acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa aggtttctgg
     9601 ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc attttccaca
     9661 gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg actcgaccat
     9721 accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt ccggctggcg
     9781 ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg ggcggcaggc
     9841 aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga gcgtttcgcc
     9901 agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat atgtgagacg
     9961 atggagcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct tttcattgag
    10021 ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct gatcactaat
    10081 ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa tctcaccctg
    10141 gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt tcgtgaccaa
    10201 ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa gcagtttttt
    10261 gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg cgatgacgtt
    10321 tccggttggg cgacggcact atagctaatc gccctctccg cccggcagaa tacccactca
    10381 gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc ggattatctg
    10441 gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt gaaaagcgcc
    10501 attttgcgct caatgaacga tgccctcatc acccgtgtga ccggcgaaga aaacgggcaa
    10561 atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga tgataccggc
    10621 gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg ccagtgggaa
    10681 ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga
    10741 tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat
    10801 attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct gctggaagag
    10861 tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt gttattgcag
    10921 gcgtggctaa tgcaaagcca acatcgctat ggcgaagtta acaccctgct agcccgtgct
    10981 gaacatgaaa tcaaggacat cagagaaggc accatgcatg cagaatttaa cgctctgcgc
    11041 gcccaggtgg cgattaacga tggtaatcag gatgaagcgg aacggctggc aaaactggca
    11101 ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc ggtgctgggt
    11161 gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctagtgca gcaaaccgaa
    11221 cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat tcagcaaagt
    11281 gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacgcagga aaaagcgttc
    11341 cagctgatca acgagcagca tctggaacag ctgccaatgc atgagtttct ggtgcgcatc
    11401 cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc ggcgcgtagc
    11461 gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct ggcaatgttg
    11521 attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagtt gaaccgtctg
    11581 gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc caacaaagtc
    11641 cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg gttgcgtcat
    11701 acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg caacattgcc
    11761 cgcgcacaaa tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga agaactcaat
    11821 gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt gctgcttaat
    11881 caactgtact ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct ggacgcatta
    11941 aaactggcga atcgcaccgg atttatcagt cattttgtca tcgaaggcga agcgatggcg
    12001 caacaactgc gccagctgat tcaacttaat acgctgccgg aactggaaca gcatcgcgcg
    12061 cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca tttcgatgag
    12121 aatttcgttg aacgtctgct aaatcatccg gaagtgccag aactaatccg caccagcccg
    12181 ttgacccaac gtgaatggca ggtcctgggg ctgatctact ccggttacag caatgaacaa
    12241 attgcgggcg aactggaagt ggcggcaacc accatcaaga cgcatatccg caatctgtac
    12301 caaaaactcg gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa actgctgaag
    12361 atgatgggtt atggggtgtg agtttagccg gataatgcat tggatccggt ttacatcttt
    12421 acatcattca atactcaccc gcgttacgcc atctgtttct atcaaaccaa accgcaccgg
    12481 caagaaacgc tccaccaccg cgatattggt cagcagatgg catgaaggat gggcgaccgt
    12541 aaattctccc gcgcccgcca gcgccatcgg cagtaccagt tggtcggcga gatattcccc
    12601 caccgccgct gggcttgcca ggtagcgttt cacctctttc actaactgcg cggctaccac
    12661 ctcggcactg acgcgctttt caccgacgac gaaaaagcgt tcagtgatat tttcactttc
    12721 gacttccagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat gaatattctg
    12781 ttcatgcagg gaaaaaatcc ccgccagtgt agcgatttca cgctcagcaa catggcgcgg
    12841 cacaccagct aatagaactt ctccacgcat ctgcacaatg ttcccgcgct cgccaagttg
    12901 caaggtgcta aacgatgcca cgggcgagac ttccgttgcc accacaccgc ctccggcagg
    12961 ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg ccagcagcgg
    13021 ctccagcacc cggcggataa aatccgcagg cggcgccgac gggttatcgg tgccgccgct
    13081 cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg tttgcagcac
    13141 cagcgtacaa cttccggcgc taccgatagc aaagcggtaa tcgccgccgc gcacggtgcc
    13201 aggccggaag accagacgct gcgatcccag ctccgcgcct tccaccgttg ccctacaaat
    13261 ttccgcagcc gcttttaccg cggtcagatg ctggcgcagc agccccggtt tcgcccgtcc
    13321 ggctcgaatg ccagtgatgg taaatggctg gccggttatc atcgacaggc tcagcgccga
    13381 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
    13441 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
    13501 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
    13561 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
    13621 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
    13681 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
    13741 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
    13801 aaagagctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
    13861 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
    13921 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
    13981 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
    14041 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
    14101 tccgcgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
    14161 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
    14221 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
    14281 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
    14341 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
    14401 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
    14461 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
    14521 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
    14581 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
    14641 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
    14701 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaattta ttgtttctaa
    14761 aaagattata tctttatatc cgtaaacgga gatttcccgc aaagccgatt taccgataat
    14821 gaaatatcgc cttttataag gatatctaag atgcgtaaaa cagtggcttt tggctttgtc
    14881 ggtaccgtgc tggattatgc cggacgcggc agtcagcgct ggtcaaaatg gcgtccgtca
    14941 ctctgtatat accagcaaga atcgttggtc atcgatcgac tggaattatt gcacgatgcc
    15001 cgctcgcgct cgctatttga aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca
    15061 gaagtggtgg gcgttgagat tgaactgcat aacccgtggg atttcgaaga ggtttacgcc
    15121 tgcctgcatg atttcgcccg tggttacgag tttcagccag aaaaagaaga ctatttaatt
    15181 cacatcacca ccggcaccca cgtcgcgcag atttgctggt ttctgctggc ggaagcgcgt
    15241 tatctacccg cccggttgat acaatcttca actccgcgca aaaaagaaca gccgcgcggc
    15301 ccaggtgaag tgacgattat cgatctcgat ttaagccgtt ataacgccat cgccagccgc
    15361 tttgccgagg aacggcagca aacgctcgat ttcctcaaat ccggtattgc cacgcgtaac
    15421 ccccacttca accgcatgat tgagcagatc gaaaaagtgg cgattaaatc ccgcgcgccg
    15481 attctgctta acggcccgac cggcgcaggt aagtcatttc tggcgcgacg catcttcgag
    15541 ttaaaacagg cacggcatca gtttagcggc gcgtttgtgg aagtgaactg cgccaccctg
    15601 cgcggcgata ccgcgatgtc gacgctgttt ggtcatgtga aaggcgcgtt taccggggcg
    15661 cgggaatctc gtgaaggatt attacgcagc gccaacggcg gaatgttgtt tcttgatgag
    15721 attggcgaac tgggcgcaga cgaacaggca atgctgctga aagctattga agagaaaacc
    15781 ttttacccgt ttggcagcga tcgccaggtg agcagtgatt ttcagcttat cgccggaacg
    15841 gtgcgcgatt tgcgccagct ggttgccgaa ggcaaattcc gcgaagatct gtacgcgcgg
    15901 atcaatctct ggaccttcac cctgccgggt ctacgccagc gccaggaaga tattgaaccg
    15961 aacctggatt atgaagtgga gcgccacgcc acactcactg gtgacagcgt gcgttttaac
    16021 accgaagcgc ggcgtgcctg gctggccttt gcaacatctc cccaggcaac atggcgcggt
    16081 aactttcgcg aactttctgc cagcgtcacg cggatggcca cctttgccac tagcggacgc
    16141 atcactctgg acgtggtgga agatgagata aaccgtctgc gctataactg gcaggagagc
    16201 cgcccctccg cgcttacggc gttgctgggc gcagaggcag aaaacatcga tctcttcgac
    16261 cgtatgcaac tggaacacgt tatcgctatc tgccgccagg caaagtcgct ttctgctgcc
    16321 ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca gcgtcaatga cgcggatcgg
    16381 ctacgcaaat acctggcgcg ttttggtctg acgtgggaag ccgtgcagga tcagcacagc
    16441 tccagttgaa tatggtggtc cgtcagcacc tgcatcacgc ttactggcgg cggggcgtcg
    16501 gtgtagacgg catcaaccat gctgatgctc cccatattga ccatcgcatt acggccaaat
    16561 tttgaatggt caaccaccag cataacgtgg cgcgagttct caataatggc gcgcttggta
    16621 cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc tgtcgatgcc gctgatcccc
    16681 agaatgccga aatcaaggcg gaactgggag ataaaatcga gcgtcgcttc gccaatgatc
    16741 ccgccatcgc ggctacgtaa ttcgccaccg gcgagaataa tgcggaaatc ttcttttacc
    16801 atcaacgtgt tagcaacgtt gagattgttg gtgacaatgc gcaaattgct gtgattgagc
    16861 agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagcgt cgagccattg
    16921 gggatttgct ccgccacttt gcgggcgatg cgctcttttt cttcggtctg ggtggcttta
    16981 cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg ccgcgccgcc atgatggcgc
    17041 aggatcaggt tttgctctgc cagctcgttg aggtcgcggc gaatagtctg cgggctgacg
    17101 gagaagtgct ccaccagttc ctcggtactg acataaccct gctgtttaac cagttcgata
    17161 ataccgttgt gacgttgtgt ttgtttcatt tataaatccc tggaattatt ttcgttttcg
    17221 cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaatccgg cgatgtgtgc
    17281 tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc agatcagcgc
    17341 aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt cgccacgtag
    17401 ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc acggcccgct
    17461 gaatttttgc tgcatatagc cgcttaacag ggcgctgata agcgtaatga caattagctt
    17521 accgctaccg aggcgttttt ccaccgcgcc gccgagatac caccaccaaa gcaggttaaa
    17581 gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa
    17641 tttcagtgtt ggatcgaatg gccaggccag ccataacatc acttcctgat cgccgagaat
    17701 ttgcatggca ataaacacca ccacgcaggc gatcatcatc acccaggtca ccggacctgc
    17761 gcgttcacgc aaggcagcaa agaaaggata acggcgataa tgcaggccac tgccggtatg
    17821 gcctgccagc cagctggccg ccagataacg cggatctgcc gggttttcga gaaaacgcgc
    17881 cagctccgcc cgcacgcgct cggcctggga ctcatccgcc agccagacat cgctttggtt
    17941 atgttgttga atcgtgagga taacaccctg cgtcgccatg taatcaacaa acgcctgcgc
    18001 cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt attccacaca
    18061 aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa ctgtcgctgc
    18121 caggcttcaa agccgccgtc aatgctatag accacatcgt agccctgttg cagcagatac
    18181 tgcgccgccc ctttgctgct attgccgtga taacacatca ccatcaccgg agtgtcaaag
    18241 tcgttatcac gcataaacgc gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca
    18301 tgccccatag cgaaactctg tggatcgcga atatcgacca gcaccgcctc tttttcctgc
    18361 aacttctggt gcgcgtcggc aacgttaata cattcgaact gatccatgcg tctctctttc
    18421 tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag ataaacgcca
    18481 taatgttata catatcactc taaaatgttt tttcaatgtt acccaaagcg cgattctttg
    18541 ctaatatgtt cgataacgaa catttatgag ctttaacgaa agtgaatgag ggcagcatgg
    18601 aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg tgctggtatc gcggcagacg
    18661 ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct tgcgcgacct
    18721 cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac tatgaattcc
    18781 gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct gaaaatggct ccgcatatcg
    18841 ccttcccgat gcgttttcgc ctgccacatc gtccgcatct gcgcccggcg tggatgattc
    18901 gcattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg ggatcaactg
    18961 gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat taagcgcgga ttcgaatatt
    19021 ctgactgttg ggtagacgac gcccgtctgg tactcgccaa cgcccagatg gtggtgcgta
    19081 aaggcggcga agtgcttact cggactcgcg ccacctctgc tcgccgcgaa aacggcctgt
    19141 ggattgtgga agcggaagat atcgataccg gcaaaaaata tagctggcaa gcgcgcggcc
    19201 tggttaacgc caccggcccg tgggtgaaac agttcttcga cgaggggatg catctgcctt
    19261 cgccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgc gtgcataccc
    19321 aaaagcaggc ctacattctg caaaacgaag ataaacgcat tgtgttcgtg atcccgtgga
    19381 tggacgagtt ttccatcatc ggcaccaccg atgtggaata caaaggtgat ccgaaagcag
    19441 tgaagattga agagagtgaa atcaattacc tgctgaaagt gtataacgcg cactttaaaa
    19501 agcagctaag ccgtgacgat atcgtctgga cctactccgg tgtgcgtccg ctgtgtgatg
    19561 atgagtccga ctcgccgcaa gccattaccc gtgattacac ccttgatatc catgatgaaa
    19621 acggcaaagc accgctgctg tcggtattcg gcggtaagct gaccacctac cgaaaactgg
    19681 cagaacatgc gctggaaaaa ctgacgccgt attatcaggg tattggcccg gcgtggacga
    19741 aagagagcgt gctgccgggt ggtgccattg aaggcgaccg cgacgattac gccgctcgcc
    19801 tgcgccgccg ttacccgttc ctgactgaat cgctggcacg tcactacacg cgcacttacg
    19861 gcagcaacag cgagctgctg ctcggcaatg cgggaacggt aagcgatctc ggggaagatt
    19921 tcggtcatga gttctacgaa gcggaactga aatacctggt ggatcacgaa tgggtccgcc
    19981 gcaccgacga tgccctgtgg cgtcgcacaa aacaaggcat gtggctgaat gcggatcaac
    20041 aatctcgtgt gagtcagtgg ctggtggaat atacgcagca gaagttgtcg ctggcgtcgt
    20101 aaattaacgt aaggtgatca ggtcagattt caatctggcc tgagactgat gacaaacaca
    20161 aaactgcctg atgcgctacg cttatcaggc ctacgtggtt tatgcaatat attgaatttg
    20221 catggtcttg taggccagat aagacgttca cgtcgcatcc ggcatgaaca aagcgcactt
    20281 tgtcaaaaat ctaacctact ttttaattca ggaaattacc gcaaagccca cgccatcata
    20341 cccaacgtaa caaaactcag gcacgttccc cactcgcccc gagaaaaata gcattaatgc
    20401 gcccagcgtc agcataaaaa tattgagcag aaggtaaatt gttggcgtaa agagtaaatt
    20461 gcaaacctca ggaatagcgg caagcccagc ccccagcatg gcaataaccg ctgcaaaaaa
    20521 ttgcgtcagt attgggatgc cgaaaaaagt cattaccagc gaggtcaaaa tccattttct
    20581 attttgcatt attctttcca ttctttctga atggcgaaat tatactcccc gagtcccctt
    20641 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccct
//
LOCUS       DAJVQR010000074        20603 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVQR010000074 DAJVQR010000000
VERSION     DAJVQR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            97..396
                     /locus_tag="OQM52_002793"
     CDS             97..396
                     /locus_tag="OQM52_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1564580.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            406..930
                     /gene="ygaP"
                     /locus_tag="OQM52_002794"
     CDS             406..930
                     /gene="ygaP"
                     /locus_tag="OQM52_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1564581.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OQM52_002795"
     CDS             complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OQM52_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1564582.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2049..2498
                     /gene="alaE"
                     /locus_tag="OQM52_002796"
     CDS             2049..2498
                     /gene="alaE"
                     /locus_tag="OQM52_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1564583.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2535..2879)
                     /locus_tag="OQM52_002797"
     CDS             complement(2535..2879)
                     /locus_tag="OQM52_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1564584.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3031..3261
                     /locus_tag="OQM52_002798"
     CDS             3031..3261
                     /locus_tag="OQM52_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564585.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="OQM52_002799"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="OQM52_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1564586.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="OQM52_002800"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="OQM52_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1564587.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="OQM52_002801"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="OQM52_002801"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1564588.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="OQM52_002802"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="OQM52_002802"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1564589.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7701..8903
                     /gene="proV"
                     /locus_tag="OQM52_002803"
     CDS             7701..8903
                     /gene="proV"
                     /locus_tag="OQM52_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1564590.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8896..9960
                     /gene="proW"
                     /locus_tag="OQM52_002804"
     CDS             8896..9960
                     /gene="proW"
                     /locus_tag="OQM52_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1564591.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10018..11010
                     /gene="proX"
                     /locus_tag="OQM52_002805"
     CDS             10018..11010
                     /gene="proX"
                     /locus_tag="OQM52_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1564592.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11202..12381
                     /locus_tag="OQM52_002806"
                     /pseudo
     CDS             11202..12381
                     /locus_tag="OQM52_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12505..13242
                     /gene="ygaZ"
                     /locus_tag="OQM52_002807"
     CDS             12505..13242
                     /gene="ygaZ"
                     /locus_tag="OQM52_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1564593.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13232..13567
                     /gene="ygaH"
                     /locus_tag="OQM52_002808"
     CDS             13232..13567
                     /gene="ygaH"
                     /locus_tag="OQM52_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1564594.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13658..14188
                     /gene="emrR"
                     /locus_tag="OQM52_002809"
     CDS             13658..14188
                     /gene="emrR"
                     /locus_tag="OQM52_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1564595.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14315..15487
                     /gene="emrA"
                     /locus_tag="OQM52_002810"
                     /pseudo
     CDS             14315..15487
                     /gene="emrA"
                     /locus_tag="OQM52_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15504..17042
                     /gene="emrB"
                     /locus_tag="OQM52_002811"
     CDS             15504..17042
                     /gene="emrB"
                     /locus_tag="OQM52_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1564596.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OQM52_002812"
     CDS             complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OQM52_002812"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1564597.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OQM52_002813"
     CDS             complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OQM52_002813"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1564598.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19400..19818)
                     /locus_tag="OQM52_002814"
                     /pseudo
     CDS             complement(19400..19818)
                     /locus_tag="OQM52_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(19815..20381)
                     /gene="yqaB"
                     /locus_tag="OQM52_002815"
     CDS             complement(19815..20381)
                     /gene="yqaB"
                     /locus_tag="OQM52_002815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1564599.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4972 a         5272 c         5145 g         5214 t
ORIGIN      
        1 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
       61 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
      121 gccagtaccg aacaggcagc ggccttattg aaagcaatga gccaccctaa acggttgctg
      181 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
      241 ggactgagcg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
      301 aacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
      361 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
      421 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
      481 gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
      541 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
      601 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
      661 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
      721 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
      781 ttaaacggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
      841 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
      901 gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga
      961 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     1021 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     1081 cagtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag
     1141 cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc
     1201 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     1261 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     1321 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     1381 taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga
     1441 tcacttatta tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag
     1501 ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag
     1561 taatttaggt tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgac
     1621 aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg
     1681 ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcatga
     1741 caaacaccga attctctacg cttatagcgt tacctccccc ccaaactttc attagtgaaa
     1801 ttaaaggcaa gatcatatct aattgattta attgaaaata aatcatattg gcatgtaaaa
     1861 atccacaatg tacaaaaaac gagccgttac ggaatatttt atctacaaaa actaactaaa
     1921 taaaaatttt tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag
     1981 atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga
     2041 gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga
     2101 tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct
     2161 tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta attgcatggc
     2221 catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct
     2281 ggataaaaaa tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg
     2341 cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca
     2401 tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac
     2461 tgtttaaagt cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat
     2521 cgccagtcgc gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca
     2581 acacgcgaac taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag
     2641 agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt
     2701 gaactgcgtt cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg
     2761 cgattaacat agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca
     2821 tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata
     2881 tttgatcctc gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct
     2941 ttcagaaaag tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga
     3001 aaattacatc gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt
     3061 gatgatggcg tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct
     3121 gtattgaagt cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct
     3181 caggcgttgc tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc
     3241 gcgcgcgatg ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg
     3301 ggtacagcag ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag
     3361 tgcaaaaatg ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt
     3421 tgcctggtga atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga
     3481 tcactatttg ctatatattg tgtagttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcaggaaac gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg
     4981 cgcgtcaggg ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccctatga cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac
     5221 actatgacga actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacagtaaa ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 atttttcccg cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag
     5701 ccctggattt caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg
     5881 gcgaactgtt tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg
     6001 ggtctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 aaaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc
     6781 agcgaaaagc tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa agtgactatt cccattgggt aatatatcga cataaacaaa
     7681 taaagaaatc attctattgc atggccatta aattaaaaat taaaaatctt tataaaatat
     7741 ttggtgagca tccacagcga gcgttcaaat atatcgaaca aggactttca aaagaacaaa
     7801 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
     7861 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
     7921 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
     7981 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
     8041 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
     8101 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     8161 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     8221 gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg
     8281 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     8341 atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc
     8401 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     8461 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt
     8521 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa actcctggct
     8581 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     8641 tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa
     8701 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     8761 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
     8821 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     8881 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     8941 agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccggc tgacggcggt
     9001 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     9061 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     9121 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     9181 ggtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc
     9241 atcacctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc
     9301 atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaactgcc
     9361 ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga
     9421 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     9481 ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc
     9541 atctttgcgc tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg
     9601 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     9661 cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggcc
     9721 ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     9781 cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc
     9841 ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     9901 aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa
     9961 ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
    10021 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct
    10081 gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa
    10141 accttccaga cgctgctggt cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa
    10201 cccagcgaag tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc
    10261 accgccgtga actggacgcc actccatgac aacatgtacg aagctgccgg tggcgataag
    10321 aaattttatc gtgaaggggt atttgttaac ggcgcagcac agggttacct gatcgataag
    10381 aaaaccgccg accaatacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc
    10441 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg
    10501 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
    10561 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
    10621 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
    10681 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat
    10741 accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc
    10801 aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg
    10861 ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
    10921 gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg
    10981 gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgttttcgc
    11041 ctcatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtccta tcatgcctac
    11101 aaaccacatc gcacatttca acaagctatc tttcatccca tattcatcaa catccgctat
    11161 tattgatttc cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg
    11221 agcttagccc ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta
    11281 actattacgc ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt
    11341 cggcaggctt tattgttacc gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg
    11401 ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag
    11461 cgggcggtat gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg
    11521 cattaaccgg tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg
    11581 cttcaccgga taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga
    11641 tattgctggc acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct
    11701 tttgggttgc ttcggtgtta atggcactga tggcgctggc attatggcgt ggtctgccac
    11761 aaatgaaatc agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt
    11821 tcatcagcga taagatcctg cgcacccgcg cgttgctggg ctgcatgacc tttgccaact
    11881 tcagcattct ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg
    11941 atggtgacat tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg
    12001 cgggcggttt tgccgacaag ggcaaatcgc accacaactt tcggtctgct tctgctgtta
    12061 ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat tatcggcatt
    12121 ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt aatttatcga
    12181 atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
    12241 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt
    12301 tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat
    12361 cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
    12421 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
    12481 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
    12541 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
    12601 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
    12661 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
    12721 gggagtagtt tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg ccatgttttg
    12781 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta
    12841 tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat
    12901 aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
    12961 gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc
    13021 gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
    13081 ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct tgcaggcgta
    13141 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
    13201 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
    13261 actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa
    13321 tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc
    13381 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
    13441 cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
    13501 tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat
    13561 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
    13621 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
    13681 tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct
    13741 tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct
    13801 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
    13861 aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc
    13921 gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgta aaagcgataa
    13981 cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
    14041 tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
    14101 agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga
    14161 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
    14221 aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct
    14281 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
    14341 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
    14401 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
    14461 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
    14521 tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgatgttct
    14581 ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc
    14641 ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat
    14701 tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
    14761 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag
    14821 tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct
    14881 ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc
    14941 ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg tttcccgtcg
    15001 tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc
    15061 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg
    15121 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
    15181 ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc aaaatgcgac
    15241 cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca
    15301 gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca ataccactaa
    15361 ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc
    15421 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
    15481 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg
    15541 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctgaac tccaccattg
    15601 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc caaggaacgt
    15661 gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
    15721 caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt
    15781 cttgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg
    15841 ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc
    15901 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt
    15961 gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca
    16021 tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg
    16081 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
    16141 tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg
    16201 aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc
    16261 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg
    16321 gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc
    16381 agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg
    16441 tagggattat tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat aaactggata
    16501 tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
    16561 atacctttga accaggtatg gatttttgcg cgtcggcctg gccgcagttt atccaggggt
    16621 ttgcggtggt ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg
    16681 aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg
    16741 gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga
    16801 ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc
    16861 ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc
    16921 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg
    16981 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact
    17041 aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt
    17101 actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg
    17161 ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag
    17221 tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc
    17281 actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc
    17341 gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc
    17401 tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta
    17461 tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg
    17521 gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc
    17581 atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt
    17641 tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc
    17701 atttgttatc atcatccctg aattcagaga tgaaattttg gccactcccg agtggccttt
    17761 ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt
    17821 ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca
    17881 gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg
    17941 ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg
    18001 gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg ccgccgttaa
    18061 tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca
    18121 gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt
    18181 caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg
    18241 gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat
    18301 ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc
    18361 cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa
    18421 tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac
    18481 cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa
    18541 ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat
    18601 tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc
    18661 actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg
    18721 ccggagatgc accaaacaga taaggaataa cccaaccgaa acgatagtaa ttgcggataa
    18781 cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact
    18841 tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca
    18901 tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt
    18961 tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac
    19021 ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca
    19081 taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt
    19141 ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac
    19201 ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct
    19261 gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg
    19321 ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac
    19381 catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa
    19441 ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc ccccaggaga
    19501 tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata
    19561 agcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg ttttacgcaa
    19621 tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac ccattgttgc
    19681 tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca ccagcacgac
    19741 ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca acgagaaaag
    19801 cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc tgcacgggcc
    19861 gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg cacgcccata
    19921 cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac gtgatcagca
    19981 gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc ctcagcgatg
    20041 gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact tttcaccact
    20101 tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc ttctgttttt
    20161 tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat tgcctgagca
    20221 atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta ctgaagaccg
    20281 tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc caggattgtg
    20341 ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc gcaagtcgaa
    20401 aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga aggagaagaa
    20461 aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag gaaatttttt
    20521 ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta
    20581 ctctatccag ctgagctacg gat
//
LOCUS       DAJVQR010000075        20521 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVQR010000075 DAJVQR010000000
VERSION     DAJVQR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OQM52_002816"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OQM52_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1564600.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OQM52_002817"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OQM52_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCS1564601.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OQM52_002818"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OQM52_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1564602.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OQM52_002819"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OQM52_002819"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1564603.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OQM52_002820"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OQM52_002820"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1564604.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OQM52_002821"
     CDS             4449..4814
                     /locus_tag="OQM52_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1564605.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OQM52_002822"
     CDS             4964..5482
                     /locus_tag="OQM52_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1564606.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OQM52_002823"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OQM52_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OQM52_002824"
     CDS             6713..7195
                     /locus_tag="OQM52_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1564607.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OQM52_002825"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OQM52_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1564608.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OQM52_002826"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OQM52_002826"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1564609.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OQM52_002827"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OQM52_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1564610.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OQM52_002828"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OQM52_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1564611.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OQM52_002829"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OQM52_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1564612.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OQM52_002830"
     CDS             10939..11730
                     /locus_tag="OQM52_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1564613.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OQM52_002831"
     CDS             11751..13037
                     /locus_tag="OQM52_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1564614.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OQM52_002832"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OQM52_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1564615.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OQM52_002833"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OQM52_002833"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1564616.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OQM52_002834"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OQM52_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1564617.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OQM52_002835"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OQM52_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1564618.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OQM52_002836"
     CDS             complement(16985..17275)
                     /locus_tag="OQM52_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1564619.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OQM52_002837"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OQM52_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1564620.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OQM52_002838"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OQM52_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1564621.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OQM52_002839"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OQM52_002839"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OQM52_002840"
     CDS             19137..20378
                     /locus_tag="OQM52_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1564622.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5357 c         4815 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAJVQR010000076        20284 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVQR010000076 DAJVQR010000000
VERSION     DAJVQR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="OQM52_002841"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="OQM52_002841"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1564623.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="OQM52_002842"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="OQM52_002842"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS1564624.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3001)
                     /locus_tag="OQM52_002843"
                     /pseudo
     CDS             complement(1729..3001)
                     /locus_tag="OQM52_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3184..4389)
                     /locus_tag="OQM52_002844"
     CDS             complement(3184..4389)
                     /locus_tag="OQM52_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564625.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4489..4845)
                     /locus_tag="OQM52_002845"
     CDS             complement(4489..4845)
                     /locus_tag="OQM52_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCS1564626.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4981..5088)
                     /gene="rprA"
                     /locus_tag="OQM52_002846"
     ncRNA           complement(4981..5088)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQM52_002846"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="OQM52_002847"
     CDS             complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="OQM52_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1564627.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6775..9831
                     /locus_tag="OQM52_002848"
     CDS             6775..9831
                     /locus_tag="OQM52_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1564628.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9828..10238
                     /gene="menI"
                     /locus_tag="OQM52_002849"
     CDS             9828..10238
                     /gene="menI"
                     /locus_tag="OQM52_002849"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1564629.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10338..10526
                     /locus_tag="OQM52_002850"
     CDS             10338..10526
                     /locus_tag="OQM52_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1564630.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11074..11442
                     /gene="sufA"
                     /locus_tag="OQM52_002851"
     CDS             11074..11442
                     /gene="sufA"
                     /locus_tag="OQM52_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1564631.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11451..12938
                     /gene="sufB"
                     /locus_tag="OQM52_002852"
     CDS             11451..12938
                     /gene="sufB"
                     /locus_tag="OQM52_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1564632.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12948..13694
                     /gene="sufC"
                     /locus_tag="OQM52_002853"
     CDS             12948..13694
                     /gene="sufC"
                     /locus_tag="OQM52_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1564633.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13669..14940
                     /gene="sufD"
                     /locus_tag="OQM52_002854"
     CDS             13669..14940
                     /gene="sufD"
                     /locus_tag="OQM52_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1564634.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14937..16157
                     /gene="sufS"
                     /locus_tag="OQM52_002855"
     CDS             14937..16157
                     /gene="sufS"
                     /locus_tag="OQM52_002855"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1564635.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16170..16586
                     /gene="sufE"
                     /locus_tag="OQM52_002856"
     CDS             16170..16586
                     /gene="sufE"
                     /locus_tag="OQM52_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1564636.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16735..17739
                     /gene="ldtE"
                     /locus_tag="OQM52_002857"
     CDS             16735..17739
                     /gene="ldtE"
                     /locus_tag="OQM52_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1564637.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="OQM52_002858"
     CDS             complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="OQM52_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1564638.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="OQM52_002859"
     CDS             complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="OQM52_002859"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1564639.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5316 a         4861 c         5175 g         4932 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg ttttgctgtt catttgcggt aattcattag
     2401 ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca
     2461 gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca
     2521 tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta
     2581 tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat
     2641 ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg
     2701 ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg
     2761 cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca
     2821 aaccgatacc ggaaattaaa taggcaatgc ccgcgttgtc agtggaaaac ttttctgaca
     2881 gagatgtcat attttgggca agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt
     2941 aaatacaaag aacagccagg ataaatggcg tgctgaaaga taatttcgtt accgtacgta
     3001 ttgttctccg acggtttaca taattactat actgaagatg attttccctg ggtacatggc
     3061 ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc
     3121 ttagttagcg cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt
     3181 gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct
     3241 gcgtaagccg ataaacagtg ccagtaaaaa accgatggca gtatcgaacc acataatatc
     3301 ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact
     3361 gcccgtactg taatagatcc ctgtagcttt acctttagcg taagggaaac gttcagccat
     3421 taacgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa
     3481 tattatcacc acataaaatg tgggatgcag gcagacggta aacagagcaa taaatgagat
     3541 aaaggtgtac agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt
     3601 aataaataca caaagcagcg aacccacggt gtagatactg agtagtttga tcgacatagt
     3661 gtatgacatg cctgcaacaa attgtccgta ctgtgccagc cactggctaa ccagataaaa
     3721 cgttgccatt gagatatagc catataaggt ataactggct aaatcgataa ttgaacagcg
     3781 atgttccgta gagctggtgg ttttctttat gacaggtaag cgacgacccg gatggggtgg
     3841 gaacgtacaa cggtataaaa acagagcgtt aataaacata atgcctgcag caatcataaa
     3901 ggaccaaccg aaccacagtt cagcccacac taacaggcta atgattagcg gtaataaaaa
     3961 ttgtccgctg gaaacaaatg ctttaattaa aatattggct gtcccaggtg agcgtggaaa
     4021 agcttccatc aaactgggat aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag
     4081 aaagccaaaa acataagcga taatgatgtt attggtctgc aggatgtcaa aaaagaaggc
     4141 catatagcag cacatcccga gcatgataaa agggcggcga ccaaagcgat cggataataa
     4201 tcctgcaaaa agcaagacac ttaatcgacc aatgcccagc gatgagataa ctatcgagac
     4261 acccgcggca ttagtctgcc aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat
     4321 gacgcccata ccatgcacca ggtaattaaa atacaaccca agtgcggtag ggtaataggg
     4381 atttttcata ggttctactt agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt
     4441 atccattcat tattgttgta acaacgacaa ttcctttctg tggaaaaatt aacaaagccg
     4501 ctccagatcg tgtgcatata attcagcggc taccgattga tagatcttcc agtcaataaa
     4561 attgccatca gggtagactt gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt
     4621 gttgccgatg cggttattta ttttctctat gatggcatga agatgttttt ttcgttgctg
     4681 acgcatagcc aacagttgct cgactacaca atcaggaatg gcgcatttgc cattttccca
     4741 ctgacgccag ctttcactgt caccggtctg ggcaatccag gtcgcgcatt catcaatagt
     4801 catggcaaaa atatggcgga gtgcctggag ttcataagcg ttcatcgtgt aatggctgca
     4861 tgaaataaac aataaaatat aaataacatt atcagcctgc tgacggcttg aagagagtca
     4921 cagtatcttg tgcaacatta ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg
     4981 ttaaaaaaaa gcccatcgtg ggagatggga aaagactaca cacagcaatt cgttgtttca
     5041 ctcaggggat ttccatgctt ataaatcaat atgttgattt ataaccgtga gctaatagta
     5101 ggcatacgga ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat
     5161 ttgcgacagg tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca
     5221 aatatcaatt agcggtgatt aacaatcagg cggcagagcc gcctgatgag aaattactta
     5281 ttcggtttct cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggtggc
     5341 gggacttctt ccacccacgc ggcaaacaga cgccaggaaa cggctaacag aaccggacca
     5401 ataaacagac cgatcatccc gaaagcaatc aaaccaccaa taacgccaga gaggatcagg
     5461 atcagcggta aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg
     5521 ccaaccacac cgctccacac taacaatacc gttccccagg tggtatcgcc ggtccagtag
     5581 agccagataa tcgccggaat cagtaccggc aacgggccaa gctggacaag gcaggagagg
     5641 atcattaaca ccgttagcaa cgttgcataa ggtacgccgg atacggcgag gccgataccg
     5701 ccaagcactg cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc
     5761 tgtgccgcca gcagcacggc ggcatctccg cgaacgcctg ccagacgggt tgcaaaatgg
     5821 cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc
     5881 agcgcacaat gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg
     5941 gtggtgccaa tataagggcg gactttcgcc atgatcgccg taccccccat atccagcaag
     6001 ttgtgccagc ctgcatacag cttcgcgcca atcaccggaa tggtattaag ccacgccaga
     6061 tcgggtaacg tcatgtcgcc gctggaaatg gctttaatta gcgggccgct gccgtcgacg
     6121 atactgttaa ccagcaaagc aatagggatg ataaacacca tcactaataa cagcgtcatc
     6181 accagaacgg cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc
     6241 caggtggcga taaccaccgt accggcccat gcaaagccga gaataaaggg ttgaacaatc
     6301 cacagacatg ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca
     6361 tccctgggct gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt
     6421 cgacgcgacg tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt
     6481 aacaggccga ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa
     6541 ctcaggcaac acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc tgcggctacg
     6601 ttcgttcacc ccagtcactt actattgtaa gctcctggag ttcactcgct tgccgccttc
     6661 ctgcaactcg aaatccatag agtaaataaa catcaccttt cagtgcgcta ttgcagccgc
     6721 ctgaaagaat agtgtatcga cctacatcac agaccgcagg aaagggtcaa tataatgatt
     6781 ccacagattt ccctggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg
     6841 gagcaacaag gttttaccgg cgatgcggcg acaagttatg ccgatcgtct gacaatgtcg
     6901 accgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca
     6961 gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct gatctttacc
     7021 ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat
     7081 atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt
     7141 gaggccgggg tgataaaaga ccaactcaat cagtacctga aaccgttcgg ctactttttt
     7201 gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca
     7261 tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg
     7321 gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgagct ggcggaaacg
     7381 ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt
     7441 cagcaacgcc agttaatcat cgacaacttc cccaaactta accgctttct taccggttac
     7501 gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg
     7561 ggttcagaag ggacgctggc ctttattacc gaagcgcggc tggatattac gcgcttgcct
     7621 aaagtgcgcc gtctggtgaa tgtcaaatat gactcttttg actctgcgct gcgtaacgcg
     7681 ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat
     7741 ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga tatgcctgac
     7801 aaagagatgc tcggactgaa cattgtggaa tttgctggtg atgatgaggc gctgattgac
     7861 gagcgagtta atgcactctg tgtgcggctt gatgagctga ttgccagcca acaggcaggt
     7921 gtgattggct ggcaggtgtg ccgcgagctg gcgggggttg aacgtatcta tgcgatgcgc
     7981 aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct
     8041 gaggatacct gcgtaccgcc ggaacatctg gccgattata ttgctgaatt tcgcgcgctg
     8101 ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac
     8161 gtccgtccgg cactggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct
     8221 gatgacgtgg tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa
     8281 ggttttcgcg ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc
     8341 aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca
     8401 gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg
     8461 cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt
     8521 ttatgcttca actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac
     8581 cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg cttgttggcg
     8641 gatcgcggcg ttgacccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg
     8701 cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc
     8761 tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcttg ttcgacccag
     8821 tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc
     8881 cgttatttac gcccgcttcg cgaccacctc gttgctacgg tcgagagcta cgcaccgctg
     8941 atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc
     9001 tcggaaaaac atatcggcat ggttgatttg ccactgctgt cggtcccctc gctacaacaa
     9061 caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca
     9121 gagcagaaag cgcgcacggt gttggtggtg caggacccct ttaccagcta ttacgatgcg
     9181 caagtggtgg cggattttgt ccgtctggtt gaaaaattag gtttccagcc tgtgttactg
     9241 ccattttcgc caaatggcaa agcccagcat atcaaaggct ttcttaatcg ttttgcgaag
     9301 acggcgaaaa agacggcgga tttcctcaac cgtatggcta agcttgggat gccaatggtg
     9361 ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc gctgggcgag
     9421 gaacgtggcg agtttaacgt cttacttgcg aatgaatggc tggcaagcgc acttgagtca
     9481 catccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaggtt
     9541 accgctttgc cgggcgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa
     9601 ctggaaaatg tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa
     9661 aaccatgaaa attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg
     9721 ccgcgtaacc gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa
     9781 ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac
     9841 ggaaaatcac cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg
     9901 atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc
     9961 ggacaaagca gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta
    10021 tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa
    10081 tcaatgctaa tcacgtccgc tcggcacgag aagggcgggt gcgcggcata tgtaaaccgt
    10141 tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt
    10201 tgtgctgttc gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga
    10261 cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt
    10321 ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt
    10381 tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag
    10441 aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact
    10501 tcgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac
    10561 ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat
    10621 attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga
    10681 ttattcttat cgccccttca agagctaagc cactgagagt gccggagata agcgccggat
    10741 ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc
    10801 tgcacttttt tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca
    10861 cagccttaat gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc
    10921 gtgcctgtta acccacacat caggggctat gcttattaaa agcaggcaaa aagaatgttt
    10981 tacggtaaag cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt
    11041 tatacgctga aagcgatgaa gtgaggtaaa tagatggaca tgcattcagg aacctttaac
    11101 ccacaggatt tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgc
    11161 gagctggtgg caaagcagcc aggtatggtc ggcgtgcgct taggtgtgaa gcaaacgggc
    11221 tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg
    11281 tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaat cgatgccgtt tattgatggc
    11341 acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa
    11401 gcccagaatg aatgtggctg tggcgaaagc tttagggtat aggcggtact atgtctcgta
    11461 atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaac tataaagaag
    11521 gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc
    11581 gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct
    11641 atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga
    11701 attatcagga ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg
    11761 cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg
    11821 aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg
    11881 ccattttcga ctctgtttcg gttgccacca cttatcgcga aaaactggcg gagcagggaa
    11941 ttattttctg ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc
    12001 tcggcaccgt ggtgccgggg aatgacaact tctttgcagc acttaatgcg gcggtagcct
    12061 ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct
    12121 attttcgcat taacgcggaa aaaaccgggc agtttgagcg cacaattctg gtggccgacg
    12181 aagacagcta cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt
    12241 tgcacgcggc agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg
    12301 tacaaaactg gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc
    12361 gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag
    12421 cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt
    12481 actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca
    12541 tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga
    12601 acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc
    12661 agtgcgactc aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat
    12721 gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac atcacgtatt ggtgaagatc
    12781 aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta
    12841 acggtttctg caaagacgtg ttctcagaac tgccgctgga atttgccgtt gaagcacaaa
    12901 aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta
    12961 aagatttaca cgtcagcgtg gaagataaag ctatcctgcg tggattaagc ctcgacgttc
    13021 gtcccggcga agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg
    13081 caacgcttgc cgggcgcgaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca
    13141 aagatttgct tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt atggccttcc
    13201 agtatccggt ggagattcca ggcgtcagta accagttttt cctgcaaacg gcacttaatg
    13261 cagtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg
    13321 aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg
    13381 gtttttccgg cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg
    13441 agttatgcat tcttgatgag tcggactccg ggctggatat tgacgcatta aaagtggtcg
    13501 ccgatggcgt gaactcgctg cgtgatggca agcgctcatt catcattgtt acgcactacc
    13561 aacgcattct cgactacatc aagcctgatt acgttcatgt gctatatcag ggacgaattg
    13621 tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta
    13681 ccgaacagca gtaatgcgct gcaacagtgg catcacttgt ttgaagctga agggacaaaa
    13741 cgctcaccgc aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt
    13801 aaacatgaaa actggaaata tacgccgctg gaagggctga ccaatagtca gtttgtcagc
    13861 attgcgggag atatatcccc acagcagcgt gatgccttag cgttaacgtt agacgccgtg
    13921 cggctggtgt ttgtcgatgg acgttacgtg cccgcgctga gcgatgcaat tgaaggcagc
    13981 ggatatgaag tgagcattaa cgacgaccgt caggggttac ccgacgctat tcaggcggaa
    14041 gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc
    14101 ggtcaacggc cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa
    14161 gaggtgaaca ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg
    14221 gtgatcgaac attttgtcag ccttaatgat gctcgtcatt ttaccggggc acggttcact
    14281 atcaatgtcg cagcgaacgc ccacttgcag catatcaagc tggcgtttga aaacccggtc
    14341 agtcaccact ttgcccataa cgatttgttg ctggctgacg atgccaccgc atttagccat
    14401 agtttcctgc tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa
    14461 aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc
    14521 cgtacctggc tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc
    14581 gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc
    14641 aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat
    14701 acaaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg
    14761 gggcgtattg atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat
    14821 gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag
    14881 gggcttaaac agcaggtgct ggcccgaatc ggtcaacggc tgccaggagg tgcaagatga
    14941 ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt
    15001 tgccgctggc ttatctcgac agcgccgcca gtgcacagaa accgagccag gtgattgacg
    15061 ccgaggccga gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa
    15121 gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg
    15181 cccgttcggc ggaagagctg gtgtttgtcc gcggcacgac ggaagggatc aatctggtcg
    15241 ccaatagctg gggcaacagc aatgtgcggg cgggcgataa catcatcatc agtcagatgg
    15301 agcaccacgc taacattgtc ccctggcaga tgctttgcgc acgcgttggc gcagagctgc
    15361 gtgtgatccc tctcaacccc gacggtacgc tgcaactgga gacgctgcct acgctgtttg
    15421 atgagaaaac tcgcctgctg gcaattactc atgtctccaa cgtgcttggc acagaaaatc
    15481 cactggcgga aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg
    15541 ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct ggattgcgac ttttacgtgt
    15601 tctccgggca taaactgtat ggccccaccg gaattggcat tctttatgtg aaagaagcct
    15661 tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga
    15721 gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg
    15781 ggggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaatagca
    15841 tagccgagta tgaacagaat ctgatgcatt atgcgctatc acagctggaa tctgtaccgg
    15901 atctcactct ctatggccaa caaaacaggc ttggcgttat tgcttttaat ctcggtaaac
    15961 accacgccta tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac
    16021 atcactgcgc tatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc
    16081 tggccatgta taacacccat gaagaagtgg agcgtctggt gaccggcctg caacgtattc
    16141 accgtctgct gggataacag ggaggcacta tggctttatt gccggataaa gaaaaattgc
    16201 tgcgtaattt tttacgctgc gccaactggg aagagaaata tctctacatt attgagctgg
    16261 gccagcgtct gccagaatta cgcgccgaag acggaagccc acaaaatagc attcagggct
    16321 gccagagtca ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt gaattgcagg
    16381 gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc
    16441 agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc
    16501 tcacccaaca tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc
    16561 gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca
    16621 ttgccggatg gtcaatgttt acactgaccg gcacaggagt gttttggttt caggtgaaca
    16681 taagcaccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa
    16741 cgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg
    16801 gcggttgatt atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat
    16861 acggtgcaag aaggggataa aaaccttcag gctattgccc gacgttttga tactgcagca
    16921 atgttgatcc ttgaagccaa taacactatc gccccggtgc caaaacctgg tacgacgata
    16981 actattcctt cacaactgtt attacctgat gcaccgcgtc aggggattat cgttaacctt
    17041 gcagagctgc gcctttatta ttatccgccg ggagaaaata ttgtgcaggt ttatccaata
    17101 ggtattggat tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg gcagaaaatc
    17161 cctaacccaa cctggacgcc tacggcaggc attcgtcagc gttcgctgga gcgtggcatt
    17221 aaattaccgc cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc
    17281 ctcgcgcatg gtaatggcga atacctcatt catggcacca gtgcgccgga cagcgttggt
    17341 ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc
    17401 agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggaa
    17461 cctaacggga tgcgttatgt tgaagtacat cgaccactat cagcagaaga acagcaaaac
    17521 gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct
    17581 gtagatcaga agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg
    17641 agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt
    17701 gaaccagagc aagggaatat gttacgcgcg acgcagtagt ggtaaatagc agacaaaaaa
    17761 atggcgcaca atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta
    17821 gccatgttgt ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg
    17881 gaacgcattg cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg
    17941 tcagaagaca gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc
    18001 aggattaccg cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag
    18061 ttaatctcca tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt
    18121 tgatgggaag gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta
    18181 gataaaaaaa ttgagagaaa acaggatgct tccatcggat tcatcttaaa taaagtgaga
    18241 ttatatagtg aaatccgatt tgatttttta ttgcttctgg ttatcgatta aataaaaaaa
    18301 gcgcccatca gggcgcttcg atatacaaat taattcacaa aagcaatatt acaggacgtg
    18361 aacagatgcg gtgttagtag tgccgctcgg taccagtgca ccagaaacca taactacgac
    18421 gtcacctttg tgtgccagac cgctctgcag agccagttct ttacccagac ggtagaaatc
    18481 atcagtagaa gtgatctctt taaccagctg cggcacaacg cctttgctca gtaccaactg
    18541 atgagccgtt ttttcgttgg tggtcagtgc caggatggtg gcatccggga agtatttacg
    18601 tactgcgcga gcagatttac cgccctgggt agcaaccacg atcagcggag catccagttt
    18661 ttcagcagtt tcaacggcac cacggcatac cgcttcggtg atgcgcagtt tacggttgtc
    18721 attgttgaac tcgagacggc tgttcatcac gcggtcggta cgttcgcaga tggtcgccat
    18781 gatagaaacc gcttccagcg ggtatttacc ttttgcggat tcaccagaca gcatcactgc
    18841 gtcagtaccg tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg
    18901 gtttttgatc atggaatcca gcatctgggt cgcagtgata acgactttac gtgcacggat
    18961 acatttttcg atcatcatct tctgggcgaa gataacttct tctaccggga tttctacacc
    19021 caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt
    19081 gaggccttcc tggttttcga ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt
    19141 caggtgctca cggatttcga taacgtcaga acgcttacga ataaaggaag cagcaacaaa
    19201 gtctacgcct ttttcgcaac caaagatcag gtcctgtttg tctttttcag ccagtgctgg
    19261 cagagcaatg gaaacgccag gcaggttcac acctttgttt tcgcccaggt caccgttgtt
    19321 cagcacttta cagataactt tgttaccttc aatggcggta acttccatac cgatcagacc
    19381 atcgtcaacc agtacggtgt tgccaacaga caggtcagta gtgaaacctt catacgttac
    19441 cgcaaccatt tcgctgttgc cgataacaga tttatcagtg gtgaaagtaa aggtctgacc
    19501 agctttcaga gaaacatcgt taccgccttc cagtttcatg gtgcggattt ccggaccttt
    19561 ggtatcaagc aggatagcgg cggttttacc agttttgctc atcacgttgc gcagattctg
    19621 aatgcgctga ccgtgttctg catagtcacc atgagagaag ttcagacgca taacgttcat
    19681 gccagcgtcc agcattttag ctaacatctc ttcagattcg gttttcggtc cgatggtgca
    19741 aacaattttg gtctttttca tgacagtctt agtctttaag ttgagaagga tgggagaaac
    19801 ttgctttctg ggcgcactgc cggaaagcga atccggtatt acgaaagtgg tggtgcctcg
    19861 ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc
    19921 aacgtgagtg tctgtttttg agtcgttgag ttctacagtc caaagtgctg aaaccattca
    19981 agagtcaatt ggcgcgcatt atacgctgaa attaatccaa aaggaaaaca aaaacagcgt
    20041 ttcaaaaaat atacaacttg ttagacaata aattgttatc aaagatttaa cgttccaggg
    20101 aaaaggttac gcaaaaactt cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg
    20161 aggataagtg aaagttcatc cggtgtataa atatatcatc gctcaattgc tacagttaaa
    20221 ataatttatc gcgttatttg tctgattttg tttgattttt gtttaattgg ttagattgat
    20281 atat
//
LOCUS       DAJVQR010000077        19308 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVQR010000077 DAJVQR010000000
VERSION     DAJVQR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="OQM52_002860"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="OQM52_002860"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCS1564640.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWACLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="OQM52_002861"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="OQM52_002861"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1564641.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OQM52_002862"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OQM52_002862"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1564642.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="OQM52_002863"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="OQM52_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1564643.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="OQM52_002864"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="OQM52_002864"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1564644.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="OQM52_002865"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="OQM52_002865"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1564645.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="OQM52_002866"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="OQM52_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1564646.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="OQM52_002867"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="OQM52_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1564647.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="OQM52_002868"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="OQM52_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1564648.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="OQM52_002869"
     CDS             11296..11511
                     /locus_tag="OQM52_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1564649.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OQM52_002870"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OQM52_002870"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1564650.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="OQM52_002871"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="OQM52_002871"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1564651.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="OQM52_002872"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="OQM52_002872"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1564652.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="OQM52_002873"
     CDS             complement(16247..16786)
                     /locus_tag="OQM52_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1564653.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="OQM52_002874"
     CDS             complement(16802..17320)
                     /locus_tag="OQM52_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1564654.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="OQM52_002875"
     CDS             complement(17631..17822)
                     /locus_tag="OQM52_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS1564655.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="OQM52_002876"
     CDS             17840..17992
                     /locus_tag="OQM52_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564656.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..>19308
                     /gene="pdeF"
                     /locus_tag="OQM52_002877"
     CDS             18174..>19308
                     /gene="pdeF"
                     /locus_tag="OQM52_002877"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1564657.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFEL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4545 a         4700 c         5251 g         4812 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt gtctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgagctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaat
//
LOCUS       DAJVQR010000078        18803 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVQR010000078 DAJVQR010000000
VERSION     DAJVQR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(139..3654)
                     /locus_tag="OQM52_002878"
     CDS             complement(139..3654)
                     /locus_tag="OQM52_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1564658.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OQM52_002879"
     CDS             complement(4107..5369)
                     /locus_tag="OQM52_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1564659.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OQM52_002880"
     tRNA            complement(5567..5642)
                     /locus_tag="OQM52_002880"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OQM52_002881"
     CDS             complement(5748..6455)
                     /locus_tag="OQM52_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1564660.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OQM52_002882"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OQM52_002882"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1564661.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OQM52_002883"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OQM52_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1564662.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OQM52_002884"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OQM52_002884"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1564663.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OQM52_002885"
     CDS             complement(11639..11914)
                     /locus_tag="OQM52_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1564664.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OQM52_002886"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OQM52_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1564665.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OQM52_002887"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OQM52_002887"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1564666.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OQM52_002888"
     CDS             13874..14200
                     /locus_tag="OQM52_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1564667.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OQM52_002889"
     CDS             14384..15103
                     /locus_tag="OQM52_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1564668.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OQM52_002890"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OQM52_002890"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1564669.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OQM52_002891"
     CDS             16442..17404
                     /locus_tag="OQM52_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1564670.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="OQM52_002892"
     CDS             complement(17505..>18803)
                     /locus_tag="OQM52_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1564671.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4584 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAJVQR010000079        18043 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVQR010000079 DAJVQR010000000
VERSION     DAJVQR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            436..1119
                     /locus_tag="OQM52_002893"
     CDS             436..1119
                     /locus_tag="OQM52_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1564672.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1163..5825
                     /locus_tag="OQM52_002894"
                     /pseudo
     CDS             1163..5825
                     /locus_tag="OQM52_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6193..7848
                     /gene="lldP"
                     /locus_tag="OQM52_002895"
     CDS             6193..7848
                     /gene="lldP"
                     /locus_tag="OQM52_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1564673.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7848..8624
                     /gene="lldR"
                     /locus_tag="OQM52_002896"
     CDS             7848..8624
                     /gene="lldR"
                     /locus_tag="OQM52_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1564674.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8621..9811
                     /gene="lldD"
                     /locus_tag="OQM52_002897"
     CDS             8621..9811
                     /gene="lldD"
                     /locus_tag="OQM52_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1564675.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10007..10480
                     /gene="trmL"
                     /locus_tag="OQM52_002898"
     CDS             10007..10480
                     /gene="trmL"
                     /locus_tag="OQM52_002898"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1564676.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10533..11354)
                     /gene="cysE"
                     /locus_tag="OQM52_002899"
     CDS             complement(10533..11354)
                     /gene="cysE"
                     /locus_tag="OQM52_002899"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1564677.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11434..12453)
                     /gene="gpsA"
                     /locus_tag="OQM52_002900"
     CDS             complement(11434..12453)
                     /gene="gpsA"
                     /locus_tag="OQM52_002900"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1564678.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12453..12920)
                     /gene="secB"
                     /locus_tag="OQM52_002901"
     CDS             complement(12453..12920)
                     /gene="secB"
                     /locus_tag="OQM52_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1564679.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12983..13234)
                     /gene="grxC"
                     /locus_tag="OQM52_002902"
     CDS             complement(12983..13234)
                     /gene="grxC"
                     /locus_tag="OQM52_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1564680.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13376..13807)
                     /locus_tag="OQM52_002903"
     CDS             complement(13376..13807)
                     /locus_tag="OQM52_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1564681.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14052..15596
                     /gene="gpmM"
                     /locus_tag="OQM52_002904"
                     /gene_synonym="pgmI"
     CDS             14052..15596
                     /gene="gpmM"
                     /locus_tag="OQM52_002904"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1564682.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15606..16889
                     /gene="envC"
                     /locus_tag="OQM52_002905"
     CDS             15606..16889
                     /gene="envC"
                     /locus_tag="OQM52_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1564683.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16893..17852
                     /locus_tag="OQM52_002906"
     CDS             16893..17852
                     /locus_tag="OQM52_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1564684.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17839..18043)
                     /locus_tag="OQM52_002907"
                     /pseudo
     CDS             complement(17839..18043)
                     /locus_tag="OQM52_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4545 a         4661 c         4674 g         4163 t
ORIGIN      
        1 ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg aaatagccat
       61 ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca tttacgtctc
      121 tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca attatctgtg
      181 aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc atcattagaa
      241 tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa tgatacattt
      301 ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt aatgtaaagc
      361 aacatagctc accaataata caatggacaa ctctaccggc attgagcgct ggtcggagga
      421 aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac tgtttcagtt
      481 ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag tagtttactg
      541 aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca agcacagcta
      601 aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag tacaaagact
      661 ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt taccgagttt
      721 aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt agcgggtcga
      781 gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact ggtcaactta
      841 ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga aaaacctgtc
      901 gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat gaaatccaca
      961 ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg tgtatcgccc
     1021 aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt cgacaatctt
     1081 ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt acgcttataa
     1141 caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga accctgcgac
     1201 agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat ctgggcgcag
     1261 taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg gggcattggc
     1321 gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag ctggcgacgg
     1381 ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca gtggttccag
     1441 tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca tcgggtcaaa
     1501 aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat cagaaggcga
     1561 tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa tggccctcgg
     1621 ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt ccaaagcgga
     1681 aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg acagcctcgc
     1741 atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg ctgagaccga
     1801 agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga ctaatagcat
     1861 ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag gaaatggcag
     1921 ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg atggcgtgga
     1981 tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg ccttaggcac
     2041 agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca gtgccaacgg
     2101 tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata ccgtctctgt
     2161 cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga tcaagtctga
     2221 cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg tagcaaaaaa
     2281 ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca ccaacctaca
     2341 atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt gatgaaaaca
     2401 ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact agtagcccaa
     2461 ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt aaggatgcgg
     2521 ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc accaatatca
     2581 ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg aacgaaacta
     2641 aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc aacgttaaag
     2701 atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg aaaaccacca
     2761 acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat attgccacta
     2821 acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag gatgcgctga
     2881 aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc accagcaaaa
     2941 taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt aacggctctc
     3001 agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc actaacacca
     3061 ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg
     3121 ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc aaaatcacca
     3181 atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt agccagcttt
     3241 atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg aatactgacg
     3301 gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg
     3361 cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg ctttgggatg
     3421 ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc gtgatcactg
     3481 atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc tcacaactcc
     3541 acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc aatgcagacg
     3601 gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat gtcggtgatg
     3661 ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg gacgccggtg
     3721 aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta atcactaacg
     3781 tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca caactctata
     3841 ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac gctgacggca
     3901 ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc ggtgacgccc
     3961 tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc ggtgctggag
     4021 cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct aatggcagta
     4081 ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg aatatgatga
     4141 ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca acctatatcg
     4201 aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta gcctttaacg
     4261 atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc gcgtctggtg
     4321 ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt attgcgctgg
     4381 gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact agcgtaacgg
     4441 aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt gcattgtcaa
     4501 ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca gaagcccatg
     4561 acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact acgccaacca
     4621 agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa gactcgctgg
     4681 caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt ttgaacactc
     4741 tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc gcaaaccatg
     4801 caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag actggctaca
     4861 ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc ggtagtgaag
     4921 acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat gcggttaacg
     4981 tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc attactaacc
     5041 tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac ggtattggcg
     5101 acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc gtagatgcca
     5161 acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc gctgacaaca
     5221 gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct gtaggttctt
     5281 ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc gatgcggtta
     5341 acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc aaagctgatg
     5401 gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg actcgtatca
     5461 gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa
     5521 gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat aacaaactgt
     5581 ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg accggtctgc
     5641 cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact tacaacggtg
     5701 aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat
     5761 tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc ggtattcagt
     5821 ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct gatgcgttac
     5881 gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc tctgatgagg
     5941 attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc aattcttcgc
     6001 ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt gccaatacat
     6061 aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc agtgccactt
     6121 ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg cgtggactct
     6181 aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa tatctggctt
     6241 tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat taagctcaaa
     6301 ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt ggctttgctg
     6361 ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt cttctatggt
     6421 ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat ctcggtgaaa
     6481 accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga ccagcgtctg
     6541 caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc aggctttggc
     6601 gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc gctgtacgcc
     6661 gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat gggcattcca
     6721 atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca gatggtgggg
     6781 cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat tatggacggc
     6841 tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc gtttgccatc
     6901 gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat ctcttcgctg
     6961 gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg cgtattccgc
     7021 ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg ttacaccgcg
     7081 ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt aacactgtgg
     7141 agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga gtgggtgatc
     7201 aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt ggtcagcgag
     7261 gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg caccgccatt
     7321 ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga cgctatcagc
     7381 accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg catggtgctg
     7441 gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact ggcactggcg
     7501 cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg ggtattcctg
     7561 accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa
     7621 caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg cgtcaccggt
     7681 aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa
     7741 gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg tatggtgggc
     7801 gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg attgttttac
     7861 ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc
     7921 tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc ggcgtatcac
     7981 gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac
     8041 gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc
     8101 agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt ctggaagccc
     8161 gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca cctggcgaca
     8221 aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat atcgcctcac
     8281 aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc gtgctgctgc
     8341 aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga
     8401 tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct gtcattgacg
     8461 ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac cttagttttg
     8521 ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg attacccgcc
     8581 tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt ccgcagccag
     8641 cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact atatggatgg
     8701 tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag aagtggcact
     8761 gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc tgtttaatga
     8821 gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt atgcgcgtcg
     8881 tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta ctctctcgac
     8941 ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa tgtggttcca
     9001 gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag caaaagcagc
     9061 gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac gctaccgtga
     9121 tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc aggcggtgac
     9181 gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt taggtaatat
     9241 ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc tggggaataa
     9301 cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct gggatggccc
     9361 gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac gttttggtgc
     9421 tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac tctcttccgc
     9481 tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc tggcggatag
     9541 cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact
     9601 gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg tagctaacct
     9661 gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg cgaaatcgat
     9721 cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc ctacggcact
     9781 ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc ctaaccctct
     9841 ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga aacgacaccg
     9901 cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa aaggccgcac
     9961 ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc taaacatcgt
    10021 actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt gcgctaatac
    10081 cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca agcgcctgcg
    10141 ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg actatcgcgc
    10201 gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag gtacgcctgc
    10261 tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg aaacacgcgg
    10321 cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca ttccgatggt
    10381 gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt atgaagcctg
    10441 gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg ccggatagat
    10501 gattacatcg catccggcac gatcacagga cattagatcc catccccata ctcaaatgta
    10561 tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct gtctggttta
    10621 ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg ttgcagcacc
    10681 acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag gattttcgcg
    10741 cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc agatttaccc
    10801 gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac cgccgtttca
    10861 cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt tgctgccggg
    10921 tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc caatgcgcga
    10981 cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg aaaacccttc
    11041 aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg caccgcctga
    11101 atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc ttcaaccact
    11161 tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag catgtagctc
    11221 agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact ggccagcatt
    11281 ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac aatttccagt
    11341 tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt tctgcgcggg
    11401 ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct cgtccttgcg
    11461 tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat ataatacttg
    11521 ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt cgcggacttc
    11581 tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc tttgtacatc
    11641 catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt cggtacaggt
    11701 aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg cacccagcgc
    11761 cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac gcgcattcgc
    11821 accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt tcaccgcgcc
    11881 gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt tgccgcagtg
    11941 cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg aaatagctgt
    12001 cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg ccagcggaat
    12061 ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt ccgcttccag
    12121 ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa tctggcgcag
    12181 cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg ctgccagcgc
    12241 agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg ggagaaacgc
    12301 ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc cccagaggac
    12361 aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg agccggcacc
    12421 gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc ttcagtacct
    12481 tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac cggcgcaagg
    12541 ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg agcatacggg
    12601 aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc ttcgataccc
    12661 gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc ttcgcccaag
    12721 gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag ttgggaagat
    12781 gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac gtgcggcgcg
    12841 ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa agtcatttca
    12901 gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa atactgcgca
    12961 atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca cgtgcatcca
    13021 atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc tggggaaccg
    13081 tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca tcgatcggca
    13141 actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg caatacgggc
    13201 aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt ttttaccctg
    13261 aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta taacaataag
    13321 tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag gtaaattatt
    13381 tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct ttcagcacga
    13441 atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac tgcatgccag
    13501 aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca acattgttgg
    13561 ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta cggaagtcat
    13621 cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc gtagcttcac
    13681 cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca agaaccgcca
    13741 ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat tgcataattt
    13801 cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa aggagtatac
    13861 ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg cggggcacga
    13921 cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat tttgatcttc
    13981 agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact cttgagtatg
    14041 aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg gatggctatg
    14101 gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg gtaatggatg
    14161 cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa gtcggtctgc
    14221 ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc ggccgcatcg
    14281 tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc tttgctaatc
    14341 cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta cacattatgg
    14401 gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg atggtagaac
    14461 tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac ggtcgcgaca
    14521 ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt gctgcgctgg
    14581 gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc gataaccgtt
    14641 gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag ttccaggccg
    14701 atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac gagttcgtga
    14761 aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac ggcgatgcgc
    14821 tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca ttcgtgaacg
    14881 ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg atgctgaccg
    14941 aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg gttaacacct
    15001 tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa accgaaaaat
    15061 atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa ggcgaagatc
    15121 gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa atgagctccg
    15181 cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac accattatct
    15241 gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg gcggttaaag
    15301 cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag tccgtgggtg
    15361 gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat ccggcaacgg
    15421 gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt ggtgataaga
    15481 acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg ttgtcgctga
    15541 tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg gaataatccc
    15601 tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg cgcaggtttg
    15661 caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg tgcgcctttt
    15721 ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc gccgcaaaag
    15781 agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa ttgaaaaagc
    15841 aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac acgctcaatc
    15901 aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag cagcaaaaag
    15961 ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag ggcgagcata
    16021 ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt ttacaggctt
    16081 atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag caaacgcgtg
    16141 aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa caaacgctgt
    16201 tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag cgtaaaaaga
    16261 cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt gaactgcgtg
    16321 ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg aaagcgcgtg
    16381 ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa gcgacgcgca
    16441 aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga actggtggcc
    16501 tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg catcgctatg
    16561 gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc tctgaaggta
    16621 ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg caaggttacg
    16681 gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc tataatcaga
    16741 gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca ctggtgggca
    16801 gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag ggtcaggcgg
    16861 tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt aacgttcttg
    16921 catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt gccatcgtca
    16981 ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg ccttccgcta
    17041 tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc aaagcgcata
    17101 acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa cagccgctgg
    17161 agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt attcgtagtg
    17221 cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc aagatgacct
    17281 ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat ctttacttcc
    17341 tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt actggcgtga
    17401 aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac atccgtgtgc
    17461 aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc attgggcatc
    17521 ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg ccagacatta
    17581 cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct acaccgccga
    17641 aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg tgcaaaccga
    17701 agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc gaaagcatca
    17761 gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc aaacagcccg
    17821 aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc gatggtgcga
    17881 ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct ggtaaccgac
    17941 cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta aatatctcgg
    18001 caatcatccg ttggcgggta agaatatccg gctctttgcg cac
//
LOCUS       DAJVQR010000080        17870 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVQR010000080 DAJVQR010000000
VERSION     DAJVQR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            68..592
                     /locus_tag="OQM52_002908"
     CDS             68..592
                     /locus_tag="OQM52_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1564685.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            673..1347
                     /locus_tag="OQM52_002909"
     CDS             673..1347
                     /locus_tag="OQM52_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1564686.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1363..3825
                     /locus_tag="OQM52_002910"
     CDS             1363..3825
                     /locus_tag="OQM52_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1564687.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3841..4874
                     /locus_tag="OQM52_002911"
                     /pseudo
     CDS             3841..4874
                     /locus_tag="OQM52_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4956..5294)
                     /gene="rcnB"
                     /locus_tag="OQM52_002912"
     CDS             complement(4956..5294)
                     /gene="rcnB"
                     /locus_tag="OQM52_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1564688.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5513..6343)
                     /gene="rcnA"
                     /locus_tag="OQM52_002913"
                     /pseudo
     CDS             complement(5513..6343)
                     /gene="rcnA"
                     /locus_tag="OQM52_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6465..6737
                     /gene="rcnR"
                     /locus_tag="OQM52_002914"
     CDS             6465..6737
                     /gene="rcnR"
                     /locus_tag="OQM52_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCS1564689.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6829..6925
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6960..7748
                     /gene="thiM"
                     /locus_tag="OQM52_002915"
     CDS             6960..7748
                     /gene="thiM"
                     /locus_tag="OQM52_002915"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1564690.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7745..8545
                     /gene="thiD"
                     /locus_tag="OQM52_002916"
     CDS             7745..8545
                     /gene="thiD"
                     /locus_tag="OQM52_002916"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1564691.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8610..9431
                     /locus_tag="OQM52_002917"
     CDS             8610..9431
                     /locus_tag="OQM52_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1564692.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9479..10225
                     /locus_tag="OQM52_002918"
     CDS             9479..10225
                     /locus_tag="OQM52_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1564693.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10199..11164)
                     /locus_tag="OQM52_002919"
     CDS             complement(10199..11164)
                     /locus_tag="OQM52_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCS1564694.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11161..12164)
                     /locus_tag="OQM52_002920"
                     /pseudo
     CDS             complement(11161..12164)
                     /locus_tag="OQM52_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12204..13256
                     /gene="fbaB"
                     /locus_tag="OQM52_002921"
     CDS             12204..13256
                     /gene="fbaB"
                     /locus_tag="OQM52_002921"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1564695.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13564..14418
                     /gene="gatY"
                     /locus_tag="OQM52_002922"
     CDS             13564..14418
                     /gene="gatY"
                     /locus_tag="OQM52_002922"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1564696.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14447..15709
                     /gene="gatZ"
                     /locus_tag="OQM52_002923"
     CDS             14447..15709
                     /gene="gatZ"
                     /locus_tag="OQM52_002923"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCS1564697.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15719..16171
                     /gene="gatA"
                     /locus_tag="OQM52_002924"
     CDS             15719..16171
                     /gene="gatA"
                     /locus_tag="OQM52_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1564698.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16202..16486
                     /gene="gatB"
                     /locus_tag="OQM52_002925"
     CDS             16202..16486
                     /gene="gatB"
                     /locus_tag="OQM52_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1564699.1"
                     /translation="MKRKIIVACGGAVATSAMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16490..17845
                     /locus_tag="OQM52_002926"
     CDS             16490..17845
                     /locus_tag="OQM52_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1564700.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4657 a         4090 c         4551 g         4572 t
ORIGIN      
        1 gagaaatttg tttccaagag atacagactc ttaacaacca aaaataaatt ttattaagga
       61 acatattatg aaacgttcaa ttattgctgc cgctgtcttt tcttcttttt ttatgagcgc
      121 tggagtattt gctgcagacg ttgataccgg aacattaacc attaaaggaa atattgcaga
      181 atctccgtgt aaattcgaag cgggtggtga ttcagtaagt attaatatgc cgactgtacc
      241 aaccactgtc tttgaaggta aagctaaata ttctacctat gatgatgcag tcggtgtaac
      301 cagcagcatg ttaaaaatta gctgcccgaa agaagttgct ggtgtaaaac tctcgttgat
      361 cacaaacgac aaaataaccg gtaacgataa ggcgatagcc agtagcaacg ataccgtagg
      421 tgataacagc gatgtcctag atgtttctgc accttttaac attgagagtt ataaaacagc
      481 ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag ataactcagt
      541 gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt aataaataaa
      601 attgcaagtt tctccaggta ataaacatgg ccgctatccc catggcggct ttttaattta
      661 agaggcatta agatgaaagg attattatct ttactcattt tttctatggt ccttcctgca
      721 catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa taaagaagtt
      781 atggtgcagt tgatgaacca gggaaatcgt tcttcgctgc tgcaggcgtg gattgatgat
      841 ggcgatacgt cgttaccacc agaaaaaatt caggttcctt tcatgttaac gccaccagtg
      901 gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc gaataaactg
      961 cccactaata aagaaagcat tttttatctg aatgttctgt atattccacc aaatagtcca
     1021 gagcaagaag gcaagaatgc actgaagttt gcgatgcaaa acagaattaa gttgttttac
     1081 cggccagcgg gtattgctcc ggtaaataaa gcgaccttta aaaagttact ggtaaatcgc
     1141 agtggcaatg gattggtgat aaaaaatgac tcagctaatt gggtgacgat ttcggatgtc
     1201 aaagctaata atgttaaagt caattatgaa actattatga ttgccccctt agaaagtcag
     1261 agtgttaata tcaaaagtaa taatgcaaat aactggtatc tgaccattat caatgaccac
     1321 ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag aatgacccca
     1381 cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc tgaagaaacc
     1441 tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa tattcgtctt
     1501 gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa taagcaatgg
     1561 cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt atcaagagaa
     1621 gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca atgtttaaca
     1681 tttgagcaac ttgttcaggg tgggagctat agctgggata tcggggtttt tcgtctcgat
     1741 ttcagtgtcc cgcaggcttg ggtggaagaa ctggaaagtg gctatgttcc accggaaaac
     1801 tgggagcggg gtattaatgc gttttatact tcttattatg tgagtcagta ttacagcgac
     1861 tataaagcgt cgggtaacaa caagagtaca tatgtacgtt ttaacagcgg gttaaattta
     1921 ctggagtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa tccaggggtg
     1981 tggaaaagca ataccctgta tctggaacgt ggatttgccc aatttctcgg cacgcttcgc
     2041 gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag cggtgtgcgg
     2101 ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa attttacgcc acgggtgcag
     2161 gggattgctc agagtaacgc gctggtaact attgaacaga atggttttgt ggtttatcag
     2221 aaagaggttc ctcctggccc gtttgcgatt acagatttgc agttggccgg tggtggagca
     2281 gatcttgatg ttagcgtgaa agaggcggac ggctcggtaa ccacctatct ggtgccttat
     2341 gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat atgattttgc ggcgggggcg
     2401 agcaagcaaa gtgattttgt ccaggcaggt tatcagtatg gttttaataa cttattgacg
     2461 ctgtatggcg gcacgatggt tgctaataat tactatgcgt ttaccctcgg aacgggttgg
     2521 aacacgcgca ttggcgcaat ttccgtcgat gccacgaagt cgcatagtaa acaagacaac
     2581 ggcgatgtgt ttgacgggca aagttatcaa attgcctaca acaaatttgt gagccaaacg
     2641 tcgacgcgtt ttggtctggc ggcctggcgt tattcgtcgc gtgattaccg gacatttaac
     2701 gatcacgttt gggcaaacaa taaagataat tatcgccgtg atgaaaacga tatctatgac
     2761 attgccgatt attaccagaa cgattttggc cgtaaaaata gcttttctgc caatatgagc
     2821 cagtcattgc cagaaggctg gggttctgtg tccttaagta cgttatggcg agattactgg
     2881 gggcgtagcg gcagcagtaa ggattatcag ttgagttatt ccaataactg gcggcggata
     2941 agctataccc tcgcggcaag ccaggcgtat ggcgagaatc atcatgaaga gaaacgtttt
     3001 aatattttta tatcaattcc ctgtgattgg ggtgatgacg ttacgacgcc tcgtcggcaa
     3061 atatatatgt ctaactcaac gacgtttgat gatcagggtt ttgcctcaaa taatacggga
     3121 ttatcaggaa cagtagggag tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa
     3181 catcagggaa atgaaacgac agctggggcg aatttgacct ggaacgcgcc ggttgcgaca
     3241 gtgaatggca gttatagtca gtcgagtact tatcgacaga ctggagccag tgtttcaggg
     3301 ggcattgtcg cctggtcggg tggcgttaat ctggcgaacc gtctttccga aacgtttgct
     3361 gtgatgaatg cgccaggaat taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca
     3421 aaccgtaatg gagtggtggt atacgacgga atgacacctt atcgggaaaa tcacctgatg
     3481 ctggatgtgt cgcaaagcga tagcgaagca gaattacgtg gcaaccggaa aattgccgcc
     3541 ccttatcgcg gcgcggttgt actggttaat tttgataccg atcagcgcaa gccctggttt
     3601 ataaaagcgt taagagcgga tggtcaacca ttaacgtttg gttatgaagt caatgatatc
     3661 catggtcata atattggcgt tgtcggccag ggaagccagt tatttattcg caccaatgaa
     3721 ataccgccat cggttaatgt agcaattgat aagcaacaag gactttcatg cacaatcacc
     3781 ttcggtaaag agattgatga aagtagaaat tatatttgcc agtaatagtc aggtggttct
     3841 atggaaattc gcataatgct atttatatta atgatgatgg ttatgcctgt gagctatgcg
     3901 gcatgttata gtgagttatc tgttcagcac aatttggttg ttcaggggaa ttttgcactt
     3961 actcaaacac aaatagcgac atatgagcat aattttaatg attcgtcatg cgtgagtaca
     4021 aatactatca cccctatgag cccatcggat attattgttg gactttataa cgataccatt
     4081 aaattaaatt tacattttga atggaccaat aaaaacaaca tcacgttgtc aaataatcag
     4141 agcagtttca ccagtggtta ttcggtaacg gtgacacctg cggccagtaa tgcaaaagtg
     4201 aatatttctg cggggggcgg tggttcagtg atgattaatg gtgttgcgac attatccagt
     4261 gcttcatcat cgacacgcgg gagtgccgca gtacaatttc tactgtgttt attaggtggc
     4321 aagtcatggg gtgcatgtgt aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc
     4381 tattccttca atctgacatt gtcatacaac ccgataacca caacctgcaa acaggacgat
     4441 ttattaatta ctttagacag tattcccgtt ttacaattac cagccacagg taacaaagca
     4501 acaataaata gtaaaaaaag ggatattatt ctgcgttgta aaaatttatt aggtcaacaa
     4561 aatcaaacat cacggaaaat gcaggtgtat ttatcaagtt ctgacttgtt aaccaacagc
     4621 aacacaatac tgaaaggtgc ggaagataat gcgtaggatt tattcttgaa agtaatggtt
     4681 cgccagtcac acttttaaat atcactaaca gcagtaaagt atacacaaat ttaaaagaaa
     4741 ttgcggcaaa gtcaaaactt acagatacaa cggtttcaat tccgataaca gccagttact
     4801 acgtctacga tacaaacaaa gttaaatctg gcgcactgga ggcaaccgca ttaatcaacg
     4861 tgaaatacga ctaataacaa gcaaaacgag caagtggcta ataataaaaa ataacaaggt
     4921 gtctgcattt ccccctcatg atgagggggc tttttttagc gatgataaaa aatctcaccg
     4981 tcgtaggctt taatgatttt accgtcggtg tcgctgatca acacgtacgc gccacccata
     5041 taggtccagt gcgtcccggc atcaggcgcg ggcaggttac gtaactgcca ctgcttaatg
     5101 ttgtactcat cggtgcggta catctctggg acggtatcac ctggtttgaa atgcttaaag
     5161 tcagcaatga aactacttaa ctcatactta gaaattcccg aggtattggt cgaacccgcg
     5221 gtggctggtg cggcccaggc ggcactggta accagcaaaa gcacacccag caacatctta
     5281 tttttaatgg tcataaactc tccactttgg ggatctgtaa tatcagtcgc atatctttcc
     5341 tgtcatacaa tataactgac aagcgatatt ttgttagtga tcccatgatt tcgtaaaaag
     5401 tgtaaatgaa aggatgccct aaaagcatcc ttagcgagca gagagtcagc ggaaatagct
     5461 gatttaagaa tcatcctggg ctgaagataa catgcaagcc taaaggattg agttatcgca
     5521 ttatgcccat gaagccatgt acgcccatat acacaccgac taagccaatc aacagactgg
     5581 aaaaataggg ggcgcgttta gcgagagtgt taaatccgct ccagcgtttt gcgacctgct
     5641 gaacgctgat tgctgcgcca acgcctacgg tgacaagcgt taacgccagg ccaatgctga
     5701 aactgacgac cagtgttgcg cccagtgtca gggctttcaa ctgaatgcat atcaacagca
     5761 cggtaattgc tgccgggcag gggataaagc caccggttaa gccaaataac aaaatttgcc
     5821 agttgatgac ctctctacca tcaaagcgtc gtttaatgtc attggcatgg gctcgtgcat
     5881 gggcatcctg atactcgcca tgttcgtgat ggtgatgatg tccttggtcg tggtgatgtt
     5941 cgtgatcgtg atgatggtga tgatgctcat aatcatgccc gtgcatattc tccagccagt
     6001 tgcgttcgcc gcgctaggta cgccagaaca tccagaacgc ggtgccaata atgatcactg
     6061 cggaaatcag ctggagccac ggttctgctg attgagcagt aaagcgctta ctgatcacca
     6121 tcccgccaaa ggcaatcaac cagaccaccg cggtatgcga aatggttgct gccagtccga
     6181 gcatcaccgc ctgtttaatg gtgcctttga tggcgatgat aaacgccgcc atcatcgttt
     6241 ttgagtgccc tggttccagg ccatgaagcg caccaagtaa gatggcgctg gggatgaaga
     6301 accaggcgtt tccttgctga agaagagttg taaattcggt catgataata attcttagta
     6361 ttaattcggc aatctgattc tactcccccc cagtacctga tactaccccc ccagtagatt
     6421 aatagtgcta tgatttttca tattcttgtt aaccaggtgt tgccatgtct catacaatcc
     6481 gtgataaaca gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc gtggcgctca
     6541 agaaaatgct cgacgagccg cacgaatgcg ctgcagtttt acaacagatt gctgctatcc
     6601 gtggagcggt aaacggtctg atgcgggaag tgattaaagg tcatctgacg gaacacatcg
     6661 ttcaccaggg ggatgagcta aaacgtgaag aagatctgga tgtcgttctg aaggtgctgg
     6721 attcatatat caaataattt attaacgcga ttgtaaaact gccgtttttc ctcgtttaca
     6781 acgggggcgc tggacattac catcctcctc tgcgatttat catcgcaacc aaacgactcg
     6841 gggtgccctt ctgcgtgaag gctgagaaat acccgtatca cctgatctgg ataatgccag
     6901 cgtagggaag tcacggacca ccaggtcatt gcttcttcac gttatggcag gagcaaacta
     6961 tgcaagtcga cctgctgagt tcagcgcaat ctgcgcacgc gttacacctt tttcaccaac
     7021 attcccctct tgtgcactgc atgaccaatg atgtggtgca aacctttacc gccaatacct
     7081 tgctggcgct cggtgcatcg ccagcgatgg ttatcgaaac cgaagaggcc agtcagtttg
     7141 cggctatcgc cagtgccttg ttgattaacg ttggcacact gacgcagcca cgcgcacagg
     7201 cgatgcgtgc tgccgttgag caagcaaaaa gctctcaaac acgctggacg cttgatccag
     7261 tagcggttgg tgcgctcgat tatcgccgcc atttttgtca tgaactttta tcttttaaac
     7321 cggcagcgat acgtggtaat gcttcggaaa tcatggcatt agctggcatt gctaatggcg
     7381 gacggggagt ggataccact gacgccgcag ctaacgcgat acccgctgca caaacactgg
     7441 cacgggaaac tggcgcaatc gtcgtggtca ctggcgaggt ggattatgtt accgatggac
     7501 atcgtatcat tggtattcac ggtggcgatc cgttaatgac caaagtggta ggaactggct
     7561 gtgcattatc ggcggttgtc gctgcctgct gtgcgttacc aggcgatacg ctggaaaatg
     7621 tcgcatctgc ctgtcactgg atgaaacaag ccggagaacg cgcagtcgcc agaagcgagg
     7681 ggccaggcag ttttgttcca catttccttg atgcgctctg gcaattgacg caggaggtgc
     7741 aggcatgaaa cgaattaacg ctctgacgat tgccggtact gatccgagtg gtggtgcggg
     7801 gattcaggcc gatcttaaaa ccttctcggc acttggcgct tatggttgct cagttattac
     7861 tgcactggtg gcgcaaaata cccgtggcgt acagtcggtg tatcgcattg agcctgattt
     7921 tgtcgccgcc cagctcgatt cggtgttcag cgatgtgcga atcgatacca ctaaaatcgg
     7981 tatgttggcg gaaaccgata ttgttgaagc ggtggcagaa cggttgcaac gttatcagat
     8041 ccaaaacgtg gtactcgaca ccgttatgct ggcaaaaagc ggcgacccgc tgctttcacc
     8101 ttcggcggtt gctacgctgc gcagtcgatt attgccacag gtttcattaa taacgccaaa
     8161 cttgcccgaa gctgccgcct tgctcgacgc gccacacgcg cgcaccgaac aggaaatgct
     8221 ggaacaaggg cgatcgctgt tggcgatggg ctgtggcgca gtgctaatga aaggtggtca
     8281 tctggatgat gagcaaagcc cggactggct gtttacccgc gagggtgaac aacggtttac
     8341 cgcaccgcgc attatgacca aaaacaccca cggcactggt tgtacactct ctgcggcgtt
     8401 ggctgcacta cgcccgcgcc atacaaactg ggctgacacc gtacaggagg caaaaatctg
     8461 gctttcatcg gcgttagccc aggccgacac gctggaagtt ggtcacggta ttggtccggt
     8521 tcaccacttc cacgcctggt ggtgagcacc gtactatagc tgctttggtt attgtcagcc
     8581 tttggcactg acattcaggg atgaaaccaa tgcagctaag aattaccagt cgaaaaaaac
     8641 tcaccgctct attatgcgct cttgggctaa tttccatcgt tgcaatttat ccgcgtcaga
     8701 cggtgaattt tttctattcg acagcagttc agattacaga ctacatccac ttctacggtt
     8761 atcgcccggt taaatctttc gctattcgca ttcctgccag ttacaccatt cacggattag
     8821 atgtttcgcg ctggcaggag cgtatcgact ggcagcgggt ggcaaaaatg cgcgacaacg
     8881 atatccgctt acagtttgct tttattaagg ctacggaagg cgaaaagctg gtggacccct
     8941 atttttcgcg taactggcaa ttaagccgcg aaaatggcct gctgcgcggg gcgtatcatt
     9001 atttctcccc gtcggtatcc gcttcagttc aggcgcgatt atttctgcaa acggtggatt
     9061 tctcacaagg cgatttccct gccgtgctgg acgtagaaga acggggaaaa ttatcggcaa
     9121 aagaattacg caagcgggta agtcagtggc taaaaatggt cgaaaaacgc acgggaaaaa
     9181 agccgattat ttactcagga gccgtttttt atcacaccaa tctggcgggc tatttcaatg
     9241 aatatccgtg gtgggtggct cactattatc aacgtcgtcc ggacaatgac ggcatggcct
     9301 ggcgcttctg gcagcattcc gaccgtggac aggtagatgg catcaatggt ccggtggatt
     9361 ttaatgtgtt taatggcacg gggatgagtt gcaggcattc gttgatggga ttaaagaaac
     9421 gccttaaata agaagcagtg atatactccc accagactcc ctggcaggaa aaagacaaat
     9481 ggaacaagcg catacccagc tgattgccca actgaacgag cgtattttag cggcggataa
     9541 cacgccgctt tatattaagt ttgccgaaac ggtaaaaaat gccgtgcgca gcggggtgct
     9601 ggagcatggc aatattttgc ccggtgagcg tgatttaagt cagttaaccg gcgtttcgcg
     9661 cattacggtg cgcaaggcga tgcaggcgct ggaagaagag ggcgtggtga cgcgttcgcg
     9721 cggttacggc acgcagatca acaacatctt cgaatactcg ttgaaagaag cgagaggttt
     9781 ttctcagcag gtggtattgc gcgggaaaaa gcccgatacg ctatgggtta ataagcgtgt
     9841 cgtgaaatgt cccgaagaag ttgcgcagca gctggcggcc gaagcgggaa gtgatgtctt
     9901 tttgcttaag cgtattcgct atgtcgatga agaagcggta tcgattgagg aatcgtgggt
     9961 tccggcgcat ttaattcatg atgttgatgc catcggaatt tctctttatg actatttccg
    10021 cagccagcac atttacccac aacgtacgcg ttcccgcgtt agcgcccgga tgccggatgc
    10081 cgagtttcag tcacatattc agttagatag caaaataccg gtgctggtga tcaagcaagt
    10141 ggcgcttgac caacagcaac ggcctattga gtacagcatt agccactgtc gcagcgatct
    10201 atacgttttt gtgtgcgagg agtagttcct cgcgcgttgg cgcacaatca ccgccccgat
    10261 gcccgacaac ccacgacgcc actgcattgc ccagtaatac ggcatccgcc agtggcagac
    10321 cagaggccag cccggcaagt acgccacctg catgactgtc gcccgcccca atggtgtcta
    10381 caacttgcgt tggaaatgcc ggaatgcagc cagaagcgtc gttgctgaaa taccatgcgc
    10441 cttctttatc gagacgaacg atcaacggcg cggcaaattt ctcctgccat tgcttgccaa
    10501 gtgttgttat ctctgcggat aaagcaaaac gttcggcggc aatctcagcc tcttgacgat
    10561 tgagtgacac taaaggtcga caggccatga tccgcgccag taatgcgtcc gggatatcgc
    10621 caatacgtgg gcaaaaatcg ataaacggcg tcacgtcttg cagcttttcc agccattcca
    10681 ctaacaattc gccgcagggc gaggccagtt gataaccgga aaaatagagc aggttgccag
    10741 gcgcaacggt taatcgtgcc aaccattggc gattccattg attttccaca ccgctgaatg
    10801 acataaaagt gcgttcgcca tccggctcaa ccagtgccag acaccagccg ttatcgcttt
    10861 cggcgttatc gatcaggctg attaacccct ctttcgccat ccggttgcga ataatctccg
    10921 cccacacgcc ctgaccgagc ggcaaggcat tacccgcttc gatgccgagg cgctttaacg
    10981 ccacggcaat attcagtgcg cagccgccaa cgttaacgct ctgctgtttc agttcgatat
    11041 cgcacccacg ccaggggagg gcataagcgt cggcgatcac gtcaatgacc gccgcgccga
    11101 cgaccatcac cggctgacgc gtggttaatt caggcagcag tgtgtgtaat cgagcgccgc
    11161 tcataccgcc tcccgttgtt gacgatattt cgccagcgct gtggcatagc ggttgaaatc
    11221 aagctgattt accgcatcca gctccgcctt taacgcagga tcgacagcgt taatgccatg
    11281 caacgcgccg caaatcgccg tcgccatagc acctatggtg tctgtgtcgc cgccaagatt
    11341 ggcgcacagg acggcgcagc ggtttgggtc ggtttgtgcc agttcaacta gcgcaatggc
    11401 gcacggaacg gactcaatag tgctggtgcc tgcgccaacg acctggtaaa gctgttcgct
    11461 ggcggattcg gtgccgtcgg cattacgcac aattttcagc gcaatctcca gacgtgccgc
    11521 cagtgaggcg ctgaacgtgg tggtgcgttt ttgctgtgca tgtcgcgcaa ttgaaggcaa
    11581 tgaatcaaca atcgctgacc agcgttctcc gtcaatggca cgagaaatcg cccatgcgat
    11641 gactaccgcg cctgcaaccg ccagatcgga tttatgggtc gggctggacg ccagcgccac
    11701 atcatcaata aaggaatcaa catcacgcgc tggaagcaaa caacctaatg gcgagacgcg
    11761 catcgctgcg ccgttggtta cgccgttgtt ttccagttca gcaacgggtt taccgtcgcg
    11821 aatggcgtta agcgcaatct tcgaggtcgg acctagtacg tttttgttaa aggcgtcgaa
    11881 acgcaacgcc cagtcgagaa tattacgccc aatcagattc ggatcgatct tgccttcaca
    11941 ttccagtaac gcatccgcca gacacaggcc atcgaggtat cgtcggtgaa ttcggcgcgg
    12001 ttaaaataac aggccgcgtt attctccttt ggtccaggca gaaaacggtc aatccagcca
    12061 aagtgtgctt taacgcggct gcgtggccaa agctcggagg gcatccccat cgcatcccct
    12121 aacgcctgcc cataaagagc accgagaata cgttctgttt tcatttaact tccccttgaa
    12181 tcggaattta cgggagcata gtaatgacag atattgcgca gttgcttggc aaagacgccg
    12241 acaacctttt acagcaccgt tgtatgacta ttccttctga ccagctttat ctccccggac
    12301 atgactacgt agaccgcgtg atgattgaca ataatcgccc gccagcggtg ttacgtaata
    12361 tgcagacgtt gtacaacacc gggcgtctgg ctggcacagg atatctttct attctgccgg
    12421 ttgaccaggg cgttgagcac tctgccggag cttcatttgc tgctaacccg ctctactttg
    12481 acccgaaaaa cattgttgaa ctggcgatcg aagcgggctg taactgtgtg gcatcaactt
    12541 acggcgtgtt ggtgtcggta tcgcggcgct atgcgcatcg cattccattt ctcgtcaaac
    12601 ttaatcacaa cgagacgcta agttacccga atacctacga tcaaacgctg tatgccagcg
    12661 tggagcaggc cttcaacatg ggcgcggtgg cggttggtgc gactatctat tttggctcgg
    12721 aagagtcacg tcgccagatt gaagaaattt ctgcggcctt tgaacgcgcg cacgagctgg
    12781 gcatggtgac agtgctgtgg gcctatttgc gaaactccgc ctttaagaaa gatggcgttg
    12841 attaccatgt ttccgccgac ctgactggtc aggcgaacca tctggcggca accataggtg
    12901 cagatatcgt caaacaaaaa atggcggaaa ataacggcgg ctataaagcg attaattacg
    12961 gttataccga cgatcgcgtg tacagcaagt taaccagcga aaacccgatt gatctggtgc
    13021 gttatcagtt agctaactgc tatatgggcc gggctgggtt gataaactcc ggcggtgctg
    13081 caggcggtga aactgacctc agcgatgcag tgcgtactgc ggttatcaac aaacgcgcag
    13141 gcggaatggg gctgattctt ggacgtaagg cgttcaagaa atcgatggct gacggcgtga
    13201 aactgattaa tgccgtgcag gacgtttatc tcgatagcaa aattactatt gcctgatacg
    13261 cctcatacga acagcaccac cccgtaggac ggataagacg tttacactgc atccggcaac
    13321 ctatgcctga tgcgacgcgg aagcgtctta tcatgcctac atagcactgc cacgtatgtt
    13381 tacaccgcat ccggcataaa aacacgcgca ctttgctacg gcttccctat cgggaggccg
    13441 tttttttgcc tttcactcct cgaacaattt tcatattgtt gtttttgcga tcattatctc
    13501 gacatttaaa agcaaataat ttcattatat tttgaaatcg aaagcaaacg acaagatatg
    13561 aaaatgtacg tggtatcgac aaagcagatg ctgaacaacg cacagcgcgg cggttatgcg
    13621 gttccggcat tcaatattca caatctcgaa acgatgcaag tggtggtgga aaccgctgcc
    13681 aacctgcatg cgccggtcat catcgccgga acgcctggca catttactca tgctggtaca
    13741 gaaaatctgt tggcgctggt cagcgcgatg gcgaagcaat atcaccatcc actggcaatt
    13801 catctcgacc atcacacgaa atttgacgat atcgctcaga aggttcgttc tggcgtgcgc
    13861 tcagtcatga ttgacgcctc gcatttgcct tttgcgcaaa atatttcacg ggtcaaagag
    13921 gtggtggatt tttgccatcg ctttgatgtc agcgtcgagg cggagctggg gcaacttggc
    13981 ggccaggaag atgatgtgca agtcaatgaa gtcgatgcgt tgtacaccaa ccccgcacag
    14041 gcgcgtgaat ttgccgaggc aaccgggatt gattccctgg cggtcgccat cggcacggct
    14101 cacggtatgt atgccagcgc accggtgctt gatttttctc gactggagaa cattcgccag
    14161 tgggtgaact taccgctggt gctgcatggc gcgtcaggat tatcgactaa ggatattcag
    14221 caaaccatca aactggggat atgcaaaatc aacgttgcaa cggagctgaa aaatgccttc
    14281 ttgcagtcgt taaaaaatta cctgaccgag caccccgaag cgaccgatcc ccgggattat
    14341 ttgcagtcgg ctaaatccgc aatgcgcgat gtggtgagca aagtgattgc cgattgtggc
    14401 tgcgagggca gggcataacg cacctgccat ttaacaagga aaaaacatga aaacgttaat
    14461 tgcccggcat aaagctggtg aacatatcgg catatgttca gtctgttctg cccatccgtt
    14521 ggttatcgaa gcggcgctgg catttgatcg caacagcacg cgcaaagtgc tgattgaagc
    14581 aacgtcaaac caggtcaatc aatttggcgg ttataccgga atgacaccgg cagactttcg
    14641 cgaatttgtt tttacgattg ccgataaagt tgggtttgca cgcgaacgca ttattctcgg
    14701 cggcgatcat ctgggaccaa actgctggca gcaagaaaat gcggatgcgg cgatggaaaa
    14761 atccgtcgag ctggtaaaag catatgttcg tgctggcttc agtaaaattc atcttgatgc
    14821 gtcaatgtcc tgcgcggggg ataccatacc gttagcacca gaaacggttg cggaacgagc
    14881 tgctgtgctt tgctttgctg cggaaagtgt ggcgacagat tgccagcgtg agcaactgag
    14941 ctatgtcatt ggcactgaag ttccggttcc gggcggtgag gccagcgcca ttcagtcagt
    15001 acacatcacc catgttgaag atgccgccaa tactttacgt acgcatcaaa aggcctttat
    15061 tgcccgtggg ctgacagagg cgttaacacg tgtgattgcc atcgtggtgc agccgggtgt
    15121 ggaatttgat cacagcaata ttatccatta tcagccgcag gaagcgcagg cgctggcgca
    15181 atggatagaa aacacccgaa tggtttatga agcacattct accgattacc agacccggac
    15241 ggcttattgg gaattagtcc gcgatcactt tgcaatattg aaagtcggtc ccgcattaac
    15301 ctttgcttta cgcgaggcga tatttgcact ggcacaaatt gagcaggaac ttatcgcccc
    15361 cgaaaatcgc agcggttgcc tggcggtaat tgaagaagtg atgctcgacg aaccgcaata
    15421 ctggaaaaaa tattatcgca cgggttttaa cgattcatta ctggatattc gttacagcct
    15481 gtcggatcgt attcgttatt actggccgca tagtcggatt aaaaatagcg ttgaaacgat
    15541 gatggtgaat ctgcaaggcg tggacatccc actgggcatg attagtcagt atcttcccaa
    15601 acaatttgaa cgcattcagt ccggggaatt atcagcaatg ccgcatcagc ttattatgaa
    15661 taaaatttat gatgttttgc gcgcctatcg ctacggctgt gcggaataag gacggtatat
    15721 gactaacctg tttgttcgta gcggaatttc ttttgtcgat cgtagcgaag ttttaaccta
    15781 tatcggtaat gagatgctcg cgaaaggtgt ggttcatgat acctggccac aggcattaat
    15841 tgccagagaa gcagaattcc ctaccgggat aatgcttgag cagcacgcca ttgcaatacc
    15901 gcattgtgag gcgattcatg ctaagtcgtc agccatttat ctgttaaggc caacaaataa
    15961 agttcatttt cagcaagcgg atgatgataa cgacgtggcg gtatcgttgg ttattgcgtt
    16021 gattgtggaa aatccgcagc agcaattgaa acttttacgc tgtttatttg gcaagttaca
    16081 acagcccgat atcgtcgaga cactaattac tcttcctgaa actcagttaa aggaatactt
    16141 cacaaagtat gttttagatt cagacgaata aatccctctg taacaataaa aaggactatt
    16201 tatgaaacgc aagattattg tcgcttgcgg aggcgcggtt gcgacctctg cgatggcggc
    16261 ggaagaaatt aaagagttgt gtcagagtca taatattcct gttgaattaa tccagtgtcg
    16321 ggttaatgaa atagaaacct atatggatgg tgtgcatttg atatgtacca ctgccagggt
    16381 ggatcgtagt tttggcgata ttccgttagt tcacggcatg ccttttgttt ctggtgtcgg
    16441 tatcgaagca ttacaaaata aaattctgac tatcttacag ggatgaccta tgttttcaga
    16501 agtcatgcgt tatattctcg acctcggccc tacagtgatg ctgccggttg tcatcattat
    16561 tttttctaaa atattaggca tgaaggcagg cgattgcttt aaagcgggtc tgcatatcgg
    16621 gattggcttt gttggcattg gccttgtgat tggcttaatg ctggattcca ttggtccggc
    16681 ggcgaaagcg atggcggaaa atttcgacct gaatctgcat gtggtcgatg tcggctggcc
    16741 gggctcttca ccaatgacct gggcgtcgca aattgcgctg gtggcgattc cgattgcgat
    16801 attggttaac gtggcgatgt tactgacccg tatgacgcgg gtggtaaatg ttgatatctg
    16861 gaatatctgg catatgacct tcaccggcgc gttgctgcac ctggcaaccg gttcatggat
    16921 gatagggatg gcgggtgtgg taattcacgc ggcgtttgtt tataagctcg gcgactggtt
    16981 tgcccgcgat acccgaaatt tctttgagct ggaaggtatt gctattccgc acggtacgtc
    17041 ggcgtatatg gggccgattg cggtgctggt cgatgctatc atcgagaaaa tcccaggcgt
    17101 taaccgaatt aaatttagcg ccgacgatat tcagcgcaaa tttggccctt ttggcgaacc
    17161 tgtcaccgtg ggttttgtga tggggctgat tatcggcatc ctcgcgggtt acgatgtcaa
    17221 aggtgtattg cagctggcgg taaaaacggc ggcagttatg ctgctgatgc cacggatgat
    17281 taaacccatc atggatggtt taacgcccat cgctaagcag gcccgtagtc gtttacaggc
    17341 gaagttcggc ggtcaggagt tcctgattgg cctggatccc gcattactgc tggggcatac
    17401 ggcggtggta tcggcaagcc tgatttttat tccgctcacc attttaattg ctgtttgtgt
    17461 tccgggtaat caggtactgc cgtttggcga tcttgccact atcggcttct tcgtggcgat
    17521 ggcggtcgcg gtgcatcgtg gaaatctgtt ccgcacctta atctcgggtg tcatcattat
    17581 gagcatcacc ctgtggatcg cgacgcaaac tattggtttg cacacccaac cggcggctaa
    17641 tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg gatcagggcg gttctcccat
    17701 tacctggtta ctgattcagg ttttctcccc gcaaaatatt cccggtttca ttattatcgg
    17761 cgcaatttat ctgaccggta ttttcatgac ctggcgtaga gcgcgtggtt ttactaaaca
    17821 agagaaagcc gttctcgcag aataattttt acctgagggg gaggttatcc
//
LOCUS       DAJVQR010000081        17602 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVQR010000081 DAJVQR010000000
VERSION     DAJVQR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(1..639)
                     /gene="chbR"
                     /locus_tag="OQM52_002927"
                     /pseudo
     CDS             complement(1..639)
                     /gene="chbR"
                     /locus_tag="OQM52_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(647..997)
                     /gene="chbA"
                     /locus_tag="OQM52_002928"
     CDS             complement(647..997)
                     /gene="chbA"
                     /locus_tag="OQM52_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS1564701.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1056..2404)
                     /gene="chbC"
                     /locus_tag="OQM52_002929"
                     /pseudo
     CDS             complement(1056..2404)
                     /gene="chbC"
                     /locus_tag="OQM52_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2489..2809)
                     /gene="chbB"
                     /locus_tag="OQM52_002930"
     CDS             complement(2489..2809)
                     /gene="chbB"
                     /locus_tag="OQM52_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1564702.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3108..3446)
                     /gene="osmE"
                     /locus_tag="OQM52_002931"
     CDS             complement(3108..3446)
                     /gene="osmE"
                     /locus_tag="OQM52_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1564703.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3648..4475
                     /gene="nadE"
                     /locus_tag="OQM52_002932"
     CDS             3648..4475
                     /gene="nadE"
                     /locus_tag="OQM52_002932"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1564704.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4705..5592
                     /gene="cho"
                     /locus_tag="OQM52_002933"
     CDS             4705..5592
                     /gene="cho"
                     /locus_tag="OQM52_002933"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1564705.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5552..6127)
                     /gene="ves"
                     /locus_tag="OQM52_002934"
     CDS             complement(5552..6127)
                     /gene="ves"
                     /locus_tag="OQM52_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1564706.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6330..6815)
                     /gene="spy"
                     /locus_tag="OQM52_002935"
     CDS             complement(6330..6815)
                     /gene="spy"
                     /locus_tag="OQM52_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1564707.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7145..8113)
                     /gene="astE"
                     /locus_tag="OQM52_002936"
     CDS             complement(7145..8113)
                     /gene="astE"
                     /locus_tag="OQM52_002936"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1564708.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8106..9439)
                     /gene="astB"
                     /locus_tag="OQM52_002937"
                     /pseudo
     CDS             complement(8106..9439)
                     /gene="astB"
                     /locus_tag="OQM52_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9436..10914)
                     /gene="astD"
                     /locus_tag="OQM52_002938"
     CDS             complement(9436..10914)
                     /gene="astD"
                     /locus_tag="OQM52_002938"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCS1564709.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10911..11945)
                     /gene="astA"
                     /locus_tag="OQM52_002939"
     CDS             complement(10911..11945)
                     /gene="astA"
                     /locus_tag="OQM52_002939"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1564710.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11942..13162)
                     /gene="astC"
                     /locus_tag="OQM52_002940"
                     /pseudo
     CDS             complement(11942..13162)
                     /gene="astC"
                     /locus_tag="OQM52_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13608..14414
                     /gene="xthA"
                     /locus_tag="OQM52_002941"
     CDS             13608..14414
                     /gene="xthA"
                     /locus_tag="OQM52_002941"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1564711.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14581..15291
                     /locus_tag="OQM52_002942"
     CDS             14581..15291
                     /locus_tag="OQM52_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1564712.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15296..15973
                     /gene="ydjY"
                     /locus_tag="OQM52_002943"
     CDS             15296..15973
                     /gene="ydjY"
                     /locus_tag="OQM52_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS1564713.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15988..16695
                     /locus_tag="OQM52_002944"
     CDS             15988..16695
                     /locus_tag="OQM52_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1564714.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16695..17243
                     /locus_tag="OQM52_002945"
     CDS             16695..17243
                     /locus_tag="OQM52_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1564715.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17253..17579
                     /locus_tag="OQM52_002946"
                     /pseudo
     CDS             17253..17579
                     /locus_tag="OQM52_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4355 a         4678 c         4300 g         4269 t
ORIGIN      
        1 atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg tcaccatatt
       61 ctccagcgcc gattcactaa actgctcttt atcatgcatc ttttctaccg tacttttcag
      121 ccactacggt acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac ggttaatgac
      181 ataaaaagta accatctcaa caaactcttc cagccctgtt tcgcggaaat ttaatgaaga
      241 aatcactgtt tccacatagg tgagaaaagc gttattggtc cggtatacct gcgaagcaac
      301 aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac tgatcccaac
      361 gttcaatatg cgcgtggcac caaactcata aaaactttga tggtgcgaac ctaacggaat
      421 aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct ggaaataacg
      481 cccggttaat accagagtaa attcataata gtcgtgctga tgcagtccgc tgatactctc
      541 agttttgtta tagataaaca catggaagtt tttgccatta aacaactgct gttctcgggc
      601 agtggcaatt tccggcgcgt taatcactgg ctgcatcatc gactccttat gccttcagtt
      661 tttcatgaag ctcaattaat tcagtaatca gttcacgcgc aagcatggac gtcattaaat
      721 gatcctgagc gtggaccagc accagactca ctttcatctt accttcgccc gcatcgcctt
      781 caatcagttt cgtctgtacc agatgcgctt cattcaatgc catccgtgac tgatccatca
      841 tggctttcgc tgcggcaaaa tcgccctgct tcgcctgttt caatgcggca tacgccaggc
      901 tacgcgcttg tccagagttg atgataagcc ccatcaccac ttcttccagc tcttcagctt
      961 ctgtttgcgt atcaggaata ttatcgagat ccatcataca tcgttcctct ctcttcttac
     1021 cggcaagatt acccgtaccg gcatcgatta aaatttcagg ggttagcgat atcttcttcg
     1081 ctctcttctt tatcaatcgc attttgtgct ttgttagcca ccacaacaaa gggcagataa
     1141 attaacgttg cgatgccaag gttgaagagt gcgaccagca atgcggcgac gctaccgttg
     1201 gtgttaaaga aggctcccag accggttggc atggtccacg gtgcaatatt ggtcaccgga
     1261 ggaatgatgc ccatgtagta cgctgcgagg gtgattgccg ccagaatcgg ttgtaccagt
     1321 acaaacggga taaacatcac cgggttcatg ataattggca gaccaaacag aatcggttcg
     1381 ttaatctgga agatgccgga cggcagcgcc agttttgcca cctgacgata atcagcacgg
     1441 cgagaggcga taaagatagc caggatcagg cctaaagtcg caccactgcc cccaaggaaa
     1501 ataaaggagt ccagcatcgg cttggcccag atatggaagg tcttaccggc tgccagcgcc
     1561 gcttcgacgg aaccatattg ctgataggtc gcgatatttt ccagcgccca cggcgtcata
     1621 atgccgttgt ccagtgcggt cagcgccagc gcgccatgaa taccgaagaa ccagagcagt
     1681 ggaacaaaga tcacataggc ccagcccacc acgctaccca acgatgccag tggggttgag
     1741 atggtatcca taatgatctg atggaagttg gtgccccagg tattcaacgc ccaggcaata
     1801 atccccatca cggaaagaat aataaagccg ggaattaatg ccgagaaaga acgcgatact
     1861 gaagcaggta cgctgtcggg cagtttaatg acccaattgc ggcggacaat aaaggtaaac
     1921 atttctgcca ccaccaggcc aataataatc ccggagatga tattcgcccc acctaaccag
     1981 tttgcgccaa ccgcataggc ctcaccgaca ctatatgggg tgacggtcat aaatgctgca
     2041 acggataaca acccagccgc cagcgcatcg actttacgct cttctgccag cgccatgcca
     2101 ataaagaacg gtgccattaa agacattatt cctaatgttc cgttatatac gttgccgcca
     2161 atacctttca gaccattaag tgtttcaatg gttgaggcgt cgaggcgaat acctaaggaa
     2221 taaaaaacga cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc
     2281 ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct
     2341 gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac
     2401 tcatagaaaa atacccttaa aaaccgcaat ttaaatatta cggtattgat ttatgaaata
     2461 actctttgac gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa
     2521 ccgcagcctt aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt
     2581 caaccggttt gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc
     2641 ctaataacac gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt
     2701 caataatgac cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag
     2761 agatagacat gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct
     2821 catcgacgat tatctgtcag ccagacactc cgcaagcctt gacctgcttc catgctctgg
     2881 gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct
     2941 gagcaatagt atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct
     3001 cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg
     3061 gcgcaatggc cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc
     3121 tgtggatcag tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta
     3181 tcatctaacg cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc
     3241 tggcaagtac cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt
     3301 gcaacctgcg cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg
     3361 tctttggtac gatcataagc tgtacaaccc gccagcatgg ttaataccgc cgctgcactc
     3421 agaattcctg ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa
     3481 gcctggaata cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg
     3541 attcctgtat gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa
     3601 cgggttagct ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac
     3661 aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc
     3721 gtagtgtcga ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc
     3781 tcgggatcag cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta
     3841 atgagctgcg ccaggaaact cgcaacgaat cactgcaatt tattgccgta cgcctgccct
     3901 atggtgttca ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc
     3961 gcgtattaac cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag
     4021 caggcattga actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag
     4081 cacaatatag cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag
     4141 aagccattac cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt
     4201 atcgtctcaa caaacgccag ggtaaacagt tactgacggc attaggttgc ccggaacacc
     4261 tttataagaa agccccaact gccgatcttg aagatgatcg cccttctctg ccagatgaag
     4321 tggcactcgg cgtgacctat gacaatatcg acgactatct ggaagggaaa aacctacctg
     4381 aacaggtcgc cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc
     4441 caattaacgt tttcgatgat ttctggaaaa agtaataatt tgcacctatt ggattgtgcg
     4501 aaaaagagta atttgttcac gccggatgcg gcgtgaacgc cttattctac ctacaaaact
     4561 atgcaaattc aatacattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg
     4621 tgtaaccttt gtcatcaacc cgcctctttt ttaatcgcct cccgcctgtt acactggata
     4681 gataaccagc attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt
     4741 gaagctgcgg caatttatga atatcccgaa catttacatt cattccttaa tgacttaccc
     4801 acccgaccag gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc
     4861 aaaagcgtta atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc
     4921 atgctacggc aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg
     4981 ctccttgaag cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc
     5041 aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa
     5101 gaggtggatt tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct
     5161 ttgcaagcat tgcagagcat cgctgatgaa caaaaacttt gttatggcct gttgggactg
     5221 gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca
     5281 tgctgcggta aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt
     5341 ttgcgagtgg tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca gcacccggaa
     5401 atgactcaat atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa
     5461 gaagcgacaa cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt
     5521 tgtaagccgc tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag
     5581 cgagccagtt gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt
     5641 gcctgccatc gaaccagcac acgccctggt cggtagtgag cagtttgtcg cccagttgcc
     5701 acgccccgtt aatcacaaac accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt
     5761 cggcaattct gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg
     5821 acatctgccc tgccgtcagt ttcgctttta ccacctggtc cgccgtaaag gcaaaaggct
     5881 gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt
     5941 ccagcaacgt cactatcctt tccatgccgg gaaacaaaga aaactcccca ttcgccgcga
     6001 tggatgcaat gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt
     6061 cgcgcgtttc accggcagcg tttcgccaca gattcaccga cattttacgc atatcaaagt
     6121 attccatact cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatgg
     6181 ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga
     6241 gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac
     6301 cggcggtctt aagttttttg gctgaaagat tattcagcag ttgcaggcat tttacctttt
     6361 gccgctggac gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc
     6421 ggcgtcagga tgttgtaaat tttgttctgg gtttccatgt gcgccagcat gttagctttg
     6481 cgctgttctt ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg
     6541 gcaatgatgt catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc
     6601 tggcctttca tgatttcgtg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg
     6661 aacatcatgt cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca
     6721 gccggtgctg cggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg
     6781 gtagaggcaa caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat
     6841 ttattacggc tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc
     6901 gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt
     6961 atggagaaaa aaagaaaatt tgatggagag tgatgagaga atattacaac acgatgattt
     7021 tgcagagatt atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga
     7081 ttaatctgta ttaatttata agaaagcaac ttaatacccg cagaatgatt tctgcgggta
     7141 agtattagct tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaacggat
     7201 tagggaataa cacatactct acatcatggg ttacggtaaa acgttcctct ccgtcctgcg
     7261 ccagcaatgt tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt
     7321 cgaaggacgg cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggtgttc
     7381 tcacgatacc gacactctca ccagatagca gcgcagcaat tgcgctggca gttactgcaa
     7441 actggcgaag atcgttttgc ccaaagggca acgctttgcc aagttccaac gtacaggcca
     7501 gcgcgccaaa atgtctgacg ctgaaatggg taaacgtacc accaggctcc tgatggaaca
     7561 ccagcgcctc cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt
     7621 cgcgttgcgg taatacaccg aactgcggat gcaaggagcc acgaattgcg gtatgtagat
     7681 caaggtgcca acgcacagat tctttgccct ggtcataaaa atcttccagg cactgttcca
     7741 gttcgcgcgc ccgacaggtt tctccgcttt cagcaaatag ctgccaacga ccgccaaaca
     7801 ttcggttcat atcgctatgg caataacgtt tcccttgctt cagcgcagga ggattcccga
     7861 ggatcaccag caaccgccaa cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg
     7921 cgtccagcat ttccacaggt gccgtctcat taccgtggat ccccgcagaa atcaccagcg
     7981 cgccttgcgg tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc
     8041 gaacgccgtt gatttctcgc tcggtgataa ccggtttttt acccgttaag gtcagagcaa
     8101 gaaaattatc cattgccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt
     8161 aattggctca atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga
     8221 tcggcagcag taagacgatc gcggtagtaa cgatccaccc agtcattgag cgcattaaac
     8281 aaggtatcgt tcatcatcac cgccggattc accgcccggc gttcttcttc tgtcaatacc
     8341 acccgcaacc gcaggcacgc cgggccgccg ccattcgcca tgctttcacg taagtcaaag
     8401 acttttagtt cgctaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact
     8461 ccggcgtgtt cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc
     8521 aacagttggc tgttaaacag ataggtagac accgcatccg acacggaaac ctgagttgcc
     8581 ggaacttcta tcgccataaa accgttcacc cgcgtgccag taactgtgac tggcgagcga
     8641 acgcctgttg gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa
     8701 aaacgctctg gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca
     8761 cctgattaag ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg
     8821 aaggccgggt atcattgcct tcttctcgcc cgtagacaaa aagtagcata cccggttcac
     8881 cgtaatgacc gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct
     8941 gcggcaacgc cgaatggacg ctaaatttct cttcgtcctt aaaaatcgct tttaacagcg
     9001 attcagtgac gggcgcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga
     9061 gatgcacttt gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc
     9121 acattggcga agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt
     9181 ccagtacctg ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac
     9241 gctcgtgcgg cggaatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca
     9301 gtaagccctg ttttgccgcc agtcgcgggt tagacacctg aaaacggtga cgggtagagg
     9361 cttcattacc aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat
     9421 taacttccca ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt
     9481 ggcgggcaat gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc
     9541 ggcataccag gcgctggggc gatggttacc ggaagcacca atgccgccga atggggcggt
     9601 actggcagca ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa
     9661 cagttgatcg aatttttccc gctcggggga aaccagaccg caaaagagtc cgaagcgagt
     9721 gttgttcgcc atccgaatcg cctcatcaaa agtatcataa cgccagacgc gcaaaaacgg
     9781 cccgaacacc tcttcatccg gcacaccgcc aacgcccgtc atttcaatga tccctggtgt
     9841 cagcaacgat gtccctgctt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc
     9901 ttccagttgc tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc
     9961 aataaacggc tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac
    10021 cagactagca agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca
    10081 ggtgcagcgt tgcccggctg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc
    10141 gatatccgcc acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat
    10201 tttctccggc tgaccggaga gctggcgatg cagctggtag ccggtattgg cgctaccggt
    10261 aaacagcaaa ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc
    10321 ctgcaccagg ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc
    10381 ttcgccactc cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa
    10441 tgccggaacg atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc
    10501 cagcacgccg tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt cactacgctg
    10561 ctcgccggta cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt
    10621 cacttcggtt gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa
    10681 ttcggcttta ttgctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc
    10741 caatgagagc cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgttcgac
    10801 ctgagcggca tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt
    10861 cacacgcgat gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt
    10921 ttctcctctg cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca
    10981 tccagttgtg cggcggttaa aatcaaacgc tcggttgccg gatcggtacg cgccagcacc
    11041 acgcggaaat ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca
    11101 ggctgccctt ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg
    11161 tcacactcaa gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct
    11221 tctttctcca gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc
    11281 tgggcttcct gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata
    11341 aatgcctttt gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag
    11401 cgtttaccga ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt
    11461 tcagcaacca ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat
    11521 ttcgacagca aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac
    11581 agctcgctgc tgccggtgtg atcgttacta agaaacagcg tcggcaatgc gttatagaca
    11641 ttcagctctt ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc
    11701 agcccaaccg ccacctcaat agcgcaaatc cccgccacgg tgcctgtctc gctatcttcc
    11761 agcacgaaca catagccctg ctcgcttttg ggcagttcac cttgccaggt tttgattgcc
    11821 ctttcgatac gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc
    11881 gttttgctgg caagctgcat cagcgccgag acatctgagc gctcaacggg acggatgacc
    11941 atcatgatga acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg
    12001 tcgtcacctc ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg
    12061 caatcagtac catcacgcct gctttcgccg cttccagaga gatctgtttc gcttgcccgg
    12121 cgtaatcggc attcagtaca cagccaatca acaaacctaa gccgcgaatt tcactgaaca
    12181 aaccatagcg gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa
    12241 cgccattaag catctctggt gtgttgatga gctccagcac tttgcctgct accgccgagg
    12301 ccagcggatt accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact
    12361 cttcggttgc caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtggtta
    12421 acagatcggg cgtcacgccg tagtgcatat aggcatacag ctcaccagtg cgcccgacgc
    12481 cggtttgtac ctcatcaaaa atcagcaagg cattgtggcg gtcacacaat tcacgcagac
    12541 cttgtaaaaa cgcgttgctg gctggcacca caccgccttc gccctagatg ggctcgacaa
    12601 tcaccgcaca ggtggcgtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag
    12661 catgacgaat atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg
    12721 cactgacagt aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccactct
    12781 tatggctgcc gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg
    12841 cttccgcacc ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt
    12901 tcgccagtcg cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg
    12961 cctgttcgtt cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa
    13021 tgccacccgc gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc
    13081 cacgtaccgg tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt
    13141 cacgcgtaat tggctgagac atagcgacct ctacagtaaa taattcgtta tttgtatgtt
    13201 aacaataagt aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata
    13261 aaagtgcata aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca
    13321 tttaaagtgc atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac
    13381 gcaatgttat gcagaagtaa aatataattc tagaattgtg atcattgacg aaatttactg
    13441 gaaattactg cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatcg
    13501 ttgacatcat taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa
    13561 ttctgctacc atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct
    13621 cttttaatat caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc
    13681 accaaccgga tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg
    13741 aagaggtggc gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg
    13801 tggcgctgct gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg
    13861 aagaggcgca gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg
    13921 tgatcaacgg ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa
    13981 aagcgcagtt ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc
    14041 cggtactgat tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg
    14101 aagaaaaccg taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg
    14161 aatggatgga caggctgatg agctgggggg tggtcgatac cttccgccat gctaatccgc
    14221 aaacagcaga tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg
    14281 gtctgcgcat cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg
    14341 gcatcgacta tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga
    14401 ccttccgccg ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg
    14461 agaaaaatca acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca
    14521 ctgcgttttt agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg
    14581 atgaacgctg agcgtaactt tctgtttgcc tgtcttattt ttgcgctggt catttacgct
    14641 atccacgctt tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatt
    14701 cgccagagcg gattgttcgg ttatagcctc tatattctgt tattcatcaa tgcccccctc
    14761 tttctgttac caggaagcat actggtgatc gctggaggaa tagtttttgg cccgctctta
    14821 gggacacaac tctcattaat tgccgcaacg ctggcctcct cgtgctcatt cctgctggcg
    14881 cgctggctgg gacgtgattt actgctgaaa tacgtgggcc atagccatac cttccaggcc
    14941 attgaaaaag gcattgcgcg taacggtatt gattttctta ttctgacccg cttaatcccg
    15001 ttgtttcctt acaatattca aaattacgct tacggattca ccaccatcgc cttctggcct
    15061 tataccctta tttcggcact cacgaccctg cccggtattg ttatttatac cgtgatggca
    15121 agcgatctcg ccaatgaagg cattacgctg cgctttattt tacaactctg tctggcgggc
    15181 ctggcgctgt ttattctcat ccagctcgca aaactctacg cccgacacaa acatgtggat
    15241 ctgtctgctt cgcgccgcag cccacttact cacccaaaaa atgaaggata gaacgatgtc
    15301 gcaacattat tcagtgtcat ggaaaaaagg actggctgca ctctgcttac tggctgttgc
    15361 cgggcttagc ggctgcgatc aaaaagagaa tgcggcggca aaagtggaat acgacggact
    15421 ttcgaacagc caaccgctgc gcgtcgatgc caataaccat acggtaacca tgctggtgca
    15481 aattaatggt cgtttcctca ccgacgacac tcgtcacggt attgtgttta aagatggctc
    15541 caacggacat aaatcgctgt ttatggctta tgcgcccccg aaagcatttt atgaagccct
    15601 gaaagaggca ggtggtacgc cgggcgaaaa catgacgatg gataataaag aaacgactca
    15661 tgtcacaggc agcaaactgg atatttcggt caactggcaa ggggcggcaa aagcgtattc
    15721 cttcgatgaa gtgattgttg acagtaatgg caagaaactg gacatgcgct ttggcggtaa
    15781 tttaacggca gcagaagaga agaaaacggg ttgcctggtg tgtctggata gctgcccggt
    15841 cggcatcgtc agcaatgcaa catacactta tggtgcggtg gaaaaacacg gcgaagtgaa
    15901 attcaaaggc aatgcctcag ttctcccggc ggataacacg ctggcaacgg ttacctttaa
    15961 aatcaccgaa taaagccaag ataaaggatg atgaagatac aatcgcgtaa aatctggtac
    16021 taccgtataa ccctcatcat cctgttgttc gctatgctgc tcgcatgggc gctgcttccc
    16081 ggcgtccatg agtttatcaa tcgcagcgtt gcggcgtttg ccgccgtgga ccaacagggt
    16141 atagaacgct ttattcagtc ttacggcgca ctggcggcag ttgtctcgtt cttgttgatg
    16201 attttacagg ccattgctgc accgctacct gcgtttttga tcacctttgc caatgcgtcg
    16261 ctgtttggcg cgttctgggg cggcttgctg tcgtggacca gttcgatggc cggcgcggcg
    16321 ctgtgctttt ttatcgccag agtgatgggc cgcgaagtgg tggaaaaatt aaccggcaaa
    16381 accgtgcttg acagtatgga cggctttttc actcgctacg gcaaacacac catcctggtc
    16441 tgtcggttat tgccttttgt ccctttcgat ccaatcagct atgctgccgg tctgacttca
    16501 atacgttttc gctcgttttt tatcgccacc gggcttggtc aattaccggc gactattgtt
    16561 tattcctggg caggcagcat gttaacaggc ggtactttct ggtttgtcac cgaactgttt
    16621 attctgtttg ccctgaccgt ggtgattttt atggcgaaga aaatatggct tgagcgccag
    16681 aagaggaatg actgatgggt ttaccgccgc ttagcaaaat tcctttcatt ttacgtccgc
    16741 aggcgtggct gcatcgtcgc cattacggcg aggtgctaag cccgattcgc tggtgggggc
    16801 ggatcccgtt tatcttttat ttggtgtcaa tgtttgttgg gtggctggag cgcaaacgct
    16861 caccgctcga tccggtagtg cgatcgcttg tcagcgcgcg cattgcgcaa atgtgcctgt
    16921 gtgagttttg cgtggatatc accagtatga aagtcgccga gcgtaccggc agcaccgata
    16981 aactgctggc agtggctgac tggcggcaaa gcccgctctt tagcgatgaa gaacggctgg
    17041 cgctggagta cgccgaagcc gcaagcgtca caccgccaac agtcgatgat gccctgcgta
    17101 cccgactggc tgcgcatttt gacgctcagg cgctcaccga actgacggca ttgatcggcc
    17161 tgcaaaacct gtcggctcgt tttaattctg ccatggacat tcccgctcag gggctgtgcc
    17221 gtattccgga aaaacgttct taaggaggga agatgcgcca ttgtgggtgg ttgctgggat
    17281 tgttatcgct gttttctttg gcaacacatg ccagtgactg gcaggaaatt aaaaatgagg
    17341 ccaaagggca aaccgtctgg tttaacgcct ggggcggcga taccgcaatt aaccgctatc
    17401 tcgactgggt tagcggcgag atgaaaaccc attacgctat aaacctgaag attgttcgtc
    17461 tcgcggatgc cgcagacgcg gtgaagcgca ttcagaccga agctgctgcc ggtcgtaaaa
    17521 cgggcggctc ggtggatctg ctctgggtga acggcgaaaa cttccgcacc ttaaaagagt
    17581 aatgccagtc agttaagcaa ct
//
LOCUS       DAJVQR010000082        17170 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVQR010000082 DAJVQR010000000
VERSION     DAJVQR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="OQM52_002947"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="OQM52_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1564716.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OQM52_002948"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OQM52_002948"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1564717.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OQM52_002949"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OQM52_002949"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1564718.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="OQM52_002950"
     CDS             complement(2205..2348)
                     /locus_tag="OQM52_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1564719.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="OQM52_002951"
     CDS             complement(3019..3306)
                     /locus_tag="OQM52_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1564720.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OQM52_002952"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OQM52_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1564721.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="OQM52_002953"
     CDS             3683..3922
                     /locus_tag="OQM52_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564722.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OQM52_002954"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OQM52_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1564723.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="OQM52_002955"
     CDS             5033..6406
                     /locus_tag="OQM52_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564724.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OQM52_002956"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OQM52_002956"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1564725.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OQM52_002957"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OQM52_002957"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1564726.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OQM52_002958"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OQM52_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1564727.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OQM52_002959"
     CDS             complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OQM52_002959"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1564728.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="OQM52_002960"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="OQM52_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1564729.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="OQM52_002961"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="OQM52_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="OQM52_002962"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="OQM52_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="OQM52_002963"
     CDS             16524..16760
                     /locus_tag="OQM52_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1564730.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16865..17056
                     /locus_tag="OQM52_002964"
     CDS             16865..17056
                     /locus_tag="OQM52_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCS1564731.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17170)
                     /locus_tag="OQM52_002965"
     CDS             complement(17081..>17170)
                     /locus_tag="OQM52_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCS1564732.1"
                     /translation="HFWFGHTPLRHRVDIGNLHYIDTGAVFGG"
BASE COUNT         4166 a         4323 c         4144 g         4537 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg
//
LOCUS       DAJVQR010000083        17128 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVQR010000083 DAJVQR010000000
VERSION     DAJVQR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..515)
                     /gene="rlmB"
                     /locus_tag="OQM52_002966"
     CDS             complement(<1..515)
                     /gene="rlmB"
                     /locus_tag="OQM52_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1564733.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIV"
     gene            complement(695..3136)
                     /gene="rnr"
                     /locus_tag="OQM52_002967"
     CDS             complement(695..3136)
                     /gene="rnr"
                     /locus_tag="OQM52_002967"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1564734.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3175..3600)
                     /gene="nsrR"
                     /locus_tag="OQM52_002968"
     CDS             complement(3175..3600)
                     /gene="nsrR"
                     /locus_tag="OQM52_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1564735.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3805..5103)
                     /gene="purA"
                     /locus_tag="OQM52_002969"
     CDS             complement(3805..5103)
                     /gene="purA"
                     /locus_tag="OQM52_002969"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1564736.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5207..5404)
                     /locus_tag="OQM52_002970"
     CDS             complement(5207..5404)
                     /locus_tag="OQM52_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1564737.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5486..6490)
                     /gene="hflC"
                     /locus_tag="OQM52_002971"
     CDS             complement(5486..6490)
                     /gene="hflC"
                     /locus_tag="OQM52_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1564738.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6493..7752)
                     /gene="hflK"
                     /locus_tag="OQM52_002972"
     CDS             complement(6493..7752)
                     /gene="hflK"
                     /locus_tag="OQM52_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1564739.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7838..9118)
                     /gene="hflX"
                     /locus_tag="OQM52_002973"
     CDS             complement(7838..9118)
                     /gene="hflX"
                     /locus_tag="OQM52_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1564740.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9194..9502)
                     /gene="hfq"
                     /locus_tag="OQM52_002974"
     CDS             complement(9194..9502)
                     /gene="hfq"
                     /locus_tag="OQM52_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1564741.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9588..10538)
                     /gene="miaA"
                     /locus_tag="OQM52_002975"
     CDS             complement(9588..10538)
                     /gene="miaA"
                     /locus_tag="OQM52_002975"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1564742.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10531..12378)
                     /gene="mutL"
                     /locus_tag="OQM52_002976"
     CDS             complement(10531..12378)
                     /gene="mutL"
                     /locus_tag="OQM52_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1564743.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12388..13725)
                     /gene="amiB"
                     /locus_tag="OQM52_002977"
     CDS             complement(12388..13725)
                     /gene="amiB"
                     /locus_tag="OQM52_002977"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1564744.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13744..14205)
                     /gene="tsaE"
                     /locus_tag="OQM52_002978"
     CDS             complement(13744..14205)
                     /gene="tsaE"
                     /locus_tag="OQM52_002978"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1564745.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14177..15724)
                     /gene="nnr"
                     /locus_tag="OQM52_002979"
     CDS             complement(14177..15724)
                     /gene="nnr"
                     /locus_tag="OQM52_002979"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1564746.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15723..16862
                     /gene="queG"
                     /locus_tag="OQM52_002980"
     CDS             15723..16862
                     /gene="queG"
                     /locus_tag="OQM52_002980"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1564747.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16845..16898)
                     /locus_tag="OQM52_002981"
     CDS             complement(16845..16898)
                     /locus_tag="OQM52_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564748.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3879 a         4882 c         4309 g         4058 t
ORIGIN      
        1 acgatccaga tattctcttc ctgcaacata cgcatggtgc gcgcaaggtt agtcacccga
       61 atcagcggaa cgctttctgc cgcgccgcag gctacttttt tcgccgtagc gttgagctgt
      121 gcggagcgat ctttcggcac aatcaccgca tgaacgcctg cggcgtccgc actacgcagg
      181 cacgcgccga ggttgtgcgg atcggttacg ccgtcgagga tcagcaggaa cggttgatcg
      241 agcgaagcga tcagatccgg cagatcgttt tcctgatact gacgtcctgg cttcacgcgg
      301 gcgataatgc cctgatgcac ggcaccgtcg cttttctcgt cgagatattg gcggtttgcc
      361 aactggataa ccacgccctg agactcaagg gcgtgaatca gcggtaacag acgtttatct
      421 tcacggcctt ttaaaataaa gacttcctga aaacgttcag gggcgcgctc cagcagggcc
      481 tgcactgcgt ggatgccgta aatcatttcg ctcattaatg tactcgttgt ttacgttttg
      541 cctgatgcac tacgtttatc cggcctacat gatctctgca atatattgaa tttgcgtgat
      601 tatgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaagcgc attttgtcag
      661 caatctaacc ctcttctttt aaagagggta ttgatcactc tgccactttt ttcttcgccg
      721 cacgcttcgc tttggtcgct gcggctattt tctgcgtttt cgccgatggc tttttcgctt
      781 ttctcgcgtc tttcttcgcc gctttcggct tcgttttttt ctcaccgcgg aaggcgctgt
      841 ctggctcaaa gtttaccttt ttaccgacct gacaacgctt gccgcctttt ttgcctgcat
      901 cgcctttttt cgctttctcg cgcgccgttt taccgacgtt gcgcggtgcg cgttcactgg
      961 agatcagact aaagtcgatt ttgcgctcgt ccatattaac cgcttcgacg cgaacttcca
     1021 cgcgatcgcc caggcgataa gtctggccgc tggattcccc catcaggcgt tgccctacct
     1081 ggtcaaagcg atagtagtca ttgtccagcg aagagacatg gaccagacca tcaatgaaca
     1141 agtcgtccag acggacgaag aagccaaagc cagtgacgct ggaaattacg cctttaaaga
     1201 cgttacctac ctggtcgagc atgaagtcac acttcagcca gtcagcgaca tcgcgcgttg
     1261 cttcgtcggc acgacgttcc gccatcgaac agtgctgacc cagttgcaac atctcttcca
     1321 tcgaataatg gtagccgccg gtttcagtgg tgttgccctg atgcccctgc tctttcgcca
     1381 gcagatattt aatggcgcgg tgcagcgtca ggtcaggata acgacgaatc ggcgaagtaa
     1441 agtgcgcata ggactgcaat gccagaccga agtgaccacg gttttctgga tcgtaaatcg
     1501 cctgtttcat cgagcgtagc agcatggttt gcagcatttc tgcatcagga cggtcggcaa
     1561 ccgactccag cagttccgcg tagtcacgcg gttccggctt gttaccaccc ggcagctcca
     1621 gccccagctc cgccagcact gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt
     1681 gaatacggaa cagtgccggt tctttcgctt tctcaacgaa acgcgccgcc gagatattcg
     1741 ccagaatcat gcactcttca attaatttgt gcgcgtcgtt acgctgggtc tgttcgatac
     1801 gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc ttcgctctca aatgagatcc
     1861 caccgcgttc ttcacgggct ttatccagca ctttatagag gttatgcaac tcttcgagat
     1921 gcttaaccag cggggcgtac tgctcgcgca gatcctgatc gccctgcaga atatgccaga
     1981 ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc ttcgtagaat ttgtagcccg
     2041 tcaggcggcc tttcgacgaa accgtcatct cgcacaccat acacaggcgg tctacctgcg
     2101 ggttgagcga acacaggccg ttagagagca cttccggcag catcgggata acctgcgaag
     2161 ggaagtacac cgacgtgcca cggttacgcg cttctctgtc cagcggcgtt ggcggacgca
     2221 catagtagct gacgtcggca atcgcgaccc ataaacgcca gccgccgccg cgttttttct
     2281 cgcagtaaac tgcatcgtca aagtcacggg cgtcttcgcc atcaatggtg accagcggta
     2341 aatcgcgcaa atcgacacgg cccgcttttg cttcttccgg cacttcttct ttcagccccg
     2401 caacctgttg ctcaacagcc tgcggccaga tatacggaat ttcatgggta cgcagagcga
     2461 tatcaaccgc catgccggtg cccatattgt cgcccagcac ttcgacgatt ttacccaccg
     2521 ctttggtgcg gcgagtcgga cgctgagtca gttcgactac gaccacaaag cccatccgcg
     2581 cgcccatgat ctgatcgggc gggattaaga tatcgaagct cagacggcta tcgtcaggaa
     2641 ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac aatctggctg gtttttggca
     2701 ccagtacgcg gacaatacgc gcttcacgac gacctttacg gtcagcaccc agcggctgcg
     2761 ccagcacctg atcgccatga atgcaggttt tcatctgctc gctggagaga tacaaatcat
     2821 ctttacgccc ttcaacccgc agaaagccgt agccatcacg gtgaccaata acggtacctt
     2881 tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg acgagtgaag accagttgac
     2941 catcgcgctc catcgcgcgc aggcggcgac gcaggccttc aagctgctct tcgccttcaa
     3001 tgtgcagttc taccgccagc tcatcacggc tggccggttt ttcacgtttg gttaaatgtt
     3061 cgaggataaa ttcccgacta gggatgggat tcgcgtattt ttcagcttcg cgttcctgga
     3121 aaggatcttg tgacatctcg gttcctccgt tgtcatctct gatgaagatt ttcgtcactc
     3181 caccagcaat aatttataaa gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt
     3241 atccagttcc gtaagaaaac tttgcacggc cttagaaagc gcctgtttca accgacaggc
     3301 aggtgtaatg tggcaaaact cactgctgca attcaccagc gataagggct ccagctcgcg
     3361 caccacatca ccaatacgta tcgcactcgc cggtttaccc aggcgaatgc cgccattttt
     3421 tccacgaaca gcagtcacgt agccggcacg actaagttga ttgattattt tgaccatatg
     3481 attacgggag acgccgtaga cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg
     3541 caatgacgcc atgtagatca gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac
     3601 atcaacctca aaagggaaat cgggaaaaaa tacatttata ttgatgataa accagccaca
     3661 agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa agcgaggatt taacactgtg
     3721 tcggatagcg taatggctta accgacacag caatgtatca cactgttagc ccggcaggca
     3781 aaatatctgc caggcgtacc agaattacgc gtcgaacggg tcgcgcagaa tcatggtttc
     3841 agtacgatcc ggaccggtag agatgatatc gatcggcaca ccggtcagct cttcaatacg
     3901 cttgatgtag ttcagcgccg cctgcggcag gccgctacga tctttcacgc cgaaagtgga
     3961 ttcagaccag cccggcatgg tttcgtaaat cggctctaca cctttccagt cgtcagctgc
     4021 cagcggagtg gtagtcactt cgcgaccatc cggcatacgg taagccacgc agagtttaac
     4081 ctctttcagg ccatccagaa cgtccagttt agtcaggcag aagccagaca gggagttcag
     4141 ctgtaccgca cgacgaacgg caacggtgtc cagccagccg gtacgacgac gacgacccgt
     4201 agttgcaccg aattcgttac cctgcttgca gaggaactcg ccagtttcat caaacagttc
     4261 ggccgggaaa ggacctgcac ctacacgagt tgagtaagct ttgaggatac ccagaacgta
     4321 atcaacataa cgcgggccca ggccggaacc ggtcgccacg ccaccagcag tggtgttgga
     4381 agaagttacg tacggataag taccgtggtc gatatccagc agcgtaccct gcgcaccttc
     4441 gaacatgacg aaatcgccac gctgacgcgc ctggtcgagc aggtcagaaa cgtcaaccac
     4501 catagaagtc aggatgtcgg caacagccat cgtatcatcc agaactttct ggtaatcaac
     4561 cgcttcagct ttgtagtagt taaccaactg gaagttgtga tattccatca cttctttcag
     4621 tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgccac
     4681 tttatcttca taagcaggcc cgataccacg accggtggtg ccgatcgctt tcgcgccacg
     4741 cgctttctca cgcgcgttat ccagagcaac gtgataatca aggatcagcg gacatgcttc
     4801 agacagcagc agacgctcac gaacggggat gccacggtct tccagttctt tcatctcttt
     4861 catcagcgcg gccggagaca gcacaacacc gttaccgatg atgctggtta cattctcgcg
     4921 gagaatacct gatggaataa gatggagaac ggttttttca ccgttgatta cgagagtatg
     4981 gcctgcgttg tgaccgccct ggtagcgtac aacatattta gcccgttcag tcagaagatc
     5041 gacgatctta cctttacctt cgtcacccca ttgggtgccc agtacgacga cgttgttacc
     5101 catttttcaa aatcaccgtt tgcttaaaaa tggattctac catcgctttt tcagagttac
     5161 agcacttttt gcactcaaaa atgacctcga tcagtctgtt ttttgttcag ccaatcgttt
     5221 tcctcaacat gtagtagacc acaacgcccg caaccacaag tccaccgcca aaacgacgta
     5281 aaatattatc gggcaaattg gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa
     5341 gcatcggccc taaaccttcc agtaccaaaa ccagggcaag cgccagccag attgtcgaat
     5401 tcattcatta tccttataga aaaagaaaac caccgacatc cctgaggatg cggtggcttt
     5461 attgacctgt accgcagtcg ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc
     5521 ggaagaaatc gctatccggg ctcatgacca tcacgtcctg attgccagag aagctgttct
     5581 cataagcacg caggctacgg atgaatgcgt agaagtccgg atctttactg aatgcatcag
     5641 caaacagttt ggctgcttcg gcatcgcctt caccacgcat aatgcggccc tgacgctcag
     5701 cttctgccag cgttctggtc acttcatagt cggcagtcgc gcgcagtttt tccgcttctt
     5761 cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg ctcggcgcgc atacggttgt
     5821 agatcgcttc agacacttcg gtcggcaggt tgatctgctt gatacgcaca tcgacaactt
     5881 caatacccag cgccgccata ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag
     5941 ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc tgccggggta gtaacttcat
     6001 cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac ttcgagggtc agacgaccac
     6061 gggaatcggt gacgatatct ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag
     6121 agaacttacg tttcaacagc acttccgctt gcgaaatgtc gccaccaccc gttgccaggt
     6181 agtaacggct gaaatcgctg atgcgccatt tgatgtaaga gtcgacgatc aggtctttct
     6241 tctctttggt cacaaagcgg tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca
     6301 ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc aggctcataa accaaaggtt
     6361 tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt aataccgcgc tcaccttctt
     6421 tgacgacaaa gacagacatg taaagcacta ccagcacgat gatgataatc gcgataactg
     6481 acttacgcat cgttattccc cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg
     6541 ttggtccata atatcgccct gactggtgga cgacgtgttg cttgctccac tggttgtgga
     6601 ggaagaggct ggcggcagac gcagcagatt gctggcaccg ttatcgctct tcgccgcagg
     6661 ggcgttacca cctttcagca tctggtctaa cggcagaacc atcaggttgc cacctttatc
     6721 gttaaccagc actttgcggg tgttacccaa cactttttcc atcgtctcga tatacagacg
     6781 ctcgcgagta atttccggcg cggctttata ttccggcaga agtttagcaa agcgcgccac
     6841 ttcaccctga gcttccagga tggtctgggc cttgtacgca cgcgcctctt cgaggatacg
     6901 ttgcgcctga ccgttcgcac gcggctgaac ttcgttggaa tacgcttctg cttcacgaat
     6961 gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc
     7021 cggcggacga gcagcctgga agttgacgtc cagcagcgtg atacccatgt catacggacg
     7081 aatcgtctct tccagttcgc gctgagtatc gctacgaatc acggtacgac cttccgtcag
     7141 aatgcggtcc atggtgtatt taccgataac tccacgcagg gcgctgtcgg tagcctgacg
     7201 caggctgtca tccgggctgg tcacgctata cagatatttt tccggattgg tgacgcggta
     7261 ctgcacgttc atctcaacgc gcactacgtt ctcgtccgac gtcagcatca caccagaagc
     7321 ggccagttca cgcacggctt ccacgttcac cggtttgact tcgtcgataa acgtcggttt
     7381 ccagttcaga cccggctcaa ccagatggct gaatttacca aagcgtgtta ccacgccgcg
     7441 ttcggcttct ttaatggtat agaaaccact ggccgcccag ataatgacaa tcgctgccgc
     7501 tgcgatggta acgacacgac cgccaagctg cgggcgcggg ccttgcgatg aactgccacc
     7561 gccagatccg gtgcctttac cgccgcccag accaccgagc tttttgctca gtttgcggaa
     7621 gatatcatct aaatcaggtg gcccttgatc gcgaccgcct ttgtttccat ttccctcaga
     7681 gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct tgtccgttat taccgggctg
     7741 attccacgcc atgtttgtgc tccatatttg ttatgcggtg atccctgttg tctttcgacg
     7801 ccagaggata tgactccacg cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg
     7861 ccggttcttg tttacagagg cgacgccagt caacgatcgg catacgaact tgcagactta
     7921 cgctgccgtc ctcctccatc cactcttttt ctattgcctg aagctgataa aaacgactcc
     7981 tcagacgccc ttcctgcggt ggcagacgca atgtatgctg cgccacctcg ccggaaagcc
     8041 gctccgtcaa agcctgaaaa agctgtggta tccccgctcc ggtctgtgcg gaaagccaga
     8101 cacggatcgg tttgttctct tcgtcccgat caatacgcgg ttcgaaatct tccagcatat
     8161 cgatcttgtt catcaccagc agggttggga tctcgtgagc gtcgatctct tcaagaaccg
     8221 tattcaccgc ttcgatgttt tcttgtacac gcacatccgc cgcatcaatg acgtgcagca
     8281 gtaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa tgccgccacc agatcgtgcg
     8341 gcaggtggcg aataaaccct acggtatctg caagtacggt ttcaccgaca tctgcaacgt
     8401 caatacgccg caacgtcggg tcgagggtgg caaacaactg gtctgccgcg tagacccgcg
     8461 cttcggtgat gcgattgaaa agggtagatt taccggcgtt ggtatatccc accagcgaaa
     8521 cagtaggaac gtcggctttg atacgcgatt gccgcccctg ctcacgctgc ttttcaactc
     8581 tttccaggcg cgactgtatc tgcacgatgc gattacgcaa caaacgacgg tcggtttcga
     8641 gctgggtttc acccggacca cgcaaaccta tcccgccttt ctgtctttca aggtgggtcc
     8701 agccacgcac caggcgcgta gccagatggc gcagctgcgc cagctcaacc tgcaacttac
     8761 cctcatgggt acgcgcacgt tgggcgaaaa tatctaaaat aaggccggtg cggtcgataa
     8821 cacgacactc gcacaaacgc tccaggttac gctcttgcgc cgggctcagg gcatggtcaa
     8881 aaagaacgac cgaagcaccc atcgctttga cagcttccgc aatttcaact gctttacctt
     8941 cacctacaaa atactttggg tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt
     9001 cgacaccggc ggaagagacc agagattcaa actcctggag gtcttccata tctttgtctt
     9061 gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc atcataacgg tcaaacaagc
     9121 gtataaccct ctaaatagat cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa
     9181 acagcccgaa accttattcg gtttcttcgc tgtcctgttg cgcggaagta ttctgcgcgc
     9241 tgctaccatg atggtagtta ctgctggtac cgccaccggc gttgttactg tgatgagaaa
     9301 ccgggcgaga tgggacaaca gtagaaatcg cgtgcttgta aaccatctgg ctgaccgtgt
     9361 ttttcaacag gatcacgaac tgatcaaaag actcgatttg cccttgcagc ttaataccat
     9421 tcaccaaata aatagaaact ggaacacgtt cccgacgcag tgcgttcagg aacggatctt
     9481 gtaaagattg ccccttagcc attctctctt ttccttatat gcttatttgt actttgaacc
     9541 tttcgattct gaaaaaattg cgcacgatac gtctcaattg tacacattca gcctgcgata
     9601 gcaccaacaa cctgtaatac ttcgtcacgc gcctgttctg gtttttcact gtcaagccag
     9661 tgaacccctt cccaaccacg cagccaggtt atctgccgct tcgccaactg tctcgtggcg
     9721 caaacacctc gataaaccat ttcatcgtat gagatttcgc cttcaaggta agaccacatc
     9781 tggcgataac ccacgcaacg aatggaaggc aagtccgtat gcaaatctcc tcgggcaaaa
     9841 agcgcccgga cttctgcttc aaaacctgaa gccaacatct gatgaaaacg ctgctcaatg
     9901 cgttgatgga gcagttcacg gctcgccggg gcgatggcga actgatgcac ctgatacggt
     9961 agagcgtctc ctgacgtttg cgtcagttcc gttaaagttt tacccgaaat gaaaaaaact
    10021 tccagtgccc gggaaagcct ttgtggatca tttggatgaa tccttgctgc cgcaaccgga
    10081 tctacctcct gaagttgacg atgcaatgac tcccaacctt gctctgccgc ctgttgctca
    10141 attctggccc gcacttccgg gtctgccgac ggtagcggcg acaacccttc cagcaatgcc
    10201 ttgaaataca acatcgtacc gcccactaac agtgggatcc gccccgccgc ggtgatatcg
    10261 gccatttccg ccagcgcatc gcggcgaaaa tcagcagccg agtaagcctg cgacggatcg
    10321 cgaatatcca gcaatcggtg cggcgcggcg agtaactctt cagcgttcgg cttcgccgtc
    10381 ccgatatcca tccctttgta aataaggaca gaatcaacgc ttatcaactc tactggtaaa
    10441 attttacgca gctcaatggc taacgccgtt ttaccggagg ccgtcggccc catcaaaaaa
    10501 atcgccttag gcaggctcgc cttactgata tcactcatct ttcagggctt ttatcgccgg
    10561 atgtaaatca acagattgta acagaccacc cggcggcgtt ttcacaagtt gcggacataa
    10621 ccgttccacg tccgccagca gggttatggc ctgtgccatt gaccactgcg catgttcgct
    10681 catcagattt cgtgcaatcc actgcgcaat attgccaggt tcgaatacgg actgcttcgc
    10741 caggtagcct atcagttcag gaatcaagat ttgtaaattt tgttggcgta agggtaaagg
    10801 cacggccctg atggtcacat gctgtgcatc tgactggaaa tcaataccca attccgctag
    10861 ggcagactgc gctttttcta atgccgattt ttcttcgcca gaaactttta gccgcaacgg
    10921 aatcagcagc ggctgggcgc aaacgggcgc ttcacccggc gtcaattgtg cctgacgcag
    10981 ccaacgttct gccactggca aggataaaag tgaaatgttg ccatcgcgct ccagcaacgc
    11041 acagtcggaa tggacgatag tcagtacccg accaaaactc tgactgttcg ccgcaagtgc
    11101 aggttcctgc ggttccggcg cttttggttt ttgcatcggc gcgggcgttt gcaaaagctg
    11161 gcgatacact tcaccttgct gtttctggta gcctggctgc gcattcggcc agggggcagc
    11221 cggacgactg cctgaggcgg gcgcaggaga gtagcgcgga gctaccggct cacgagctgc
    11281 cggttctgca aagtgattgc gccccgccgc cacgcggttt tccgggatgg cacgcggtgc
    11341 gggttgtggt tcatcgtcca gcggtagcgg cgtttccagt tgctgttgta gcacgctcag
    11401 cacgccctga tagataaaat catgcaccag acgcgactga tggaaacgca cttcgtgttt
    11461 ggcggggtgt acgttgacgt ccacctgatg cgggtcgatc tccagataca acacaaatgc
    11521 cggttgctga tcggccccca gtttgtcttc gcaggcctgg cggatcgcat gattgatcag
    11581 gcgatcgcgc atcatgcgac cattcacgta gcaatactga atttccgcca gtgcgggcgt
    11641 agtgtgattt ggatcggcca cccagccgcg cagggtgaga tcgccgtgtt gccattcaat
    11701 cgccagcgct tgttcgagaa aagcggtgcc gcaaatcgcg cctaagcgcc gttctttttg
    11761 cccgccttcc ggcactgcgc ggtactgacg cacaatttta ccgttatgcg acaggttgat
    11821 cgtgacgtcg aaacgcgcca gcgcaatgcg gcggatgatt tcatcaatat ggctaaattc
    11881 ggttttctcg gtgcgcagga atttgcgccg cgccggggtg ttgtagaaca gatccagcac
    11941 ctccagcgtc gttcccaccg gatgcgctgc cggtttaacc gtcacgtcca tgtcgcgtcc
    12001 ttcggcatag gcctgccagg cttcctgctg ttctgcggtg cgtgaagtga gcgtcaggcg
    12061 ggaaaccgaa ctgatactcg ccagcgcctc accgcgaaag cccaggctga taatggcttc
    12121 gagatcgtcc agagaggcga ttttactggt ggcatgacga gccagcgcca gcgccagctc
    12181 atcttttttg ataccgcagc cgttatcacg aatgcggata agtttcgccc caccgcgttc
    12241 gatatcaata tcgatacgcg tcgcacctgc atcgaggctg ttttccacta gttctttgac
    12301 taccgacgca ggtcgctcga ccacctcacc tgcggcaatc tggttcgcca gttgtggcgg
    12361 taagacctga attggcatca atcgtcctta gtttggcagc gtgcgatctg gcgtcgtcac
    12421 cgtactggca gtttgtgccg ttgcaccctg cggcgcagat tgcatcggat gcgcaaggaa
    12481 gtaattgcgc aggcctttgt aaatggcttc tgccagctgt tgttggtaat cgtcgctcgc
    12541 cagcaaacgt tcttcgctgt tgttgctgat aaaaccggtt tcgaccagta ctgatgggat
    12601 atccggcgag cgcagaacgc caaggctggc gtgttctggt cgacgtttat gtatttcgcc
    12661 aatgcgttgc aactgactga tcatactggt cgctacatca taccctaccc gctgggaatg
    12721 accgaactgt aaatccagca ccgcctggct taaatagggg tcagactgac tgttcgccag
    12781 cacatcaccc gccccaccca gcagctccga cagtttctcg tgctgctcca gccagctggc
    12841 catttcactg ttggcgcgac ggttagagag cacccatacg gaagcgccag tcgcactacg
    12901 gttcggtgcg gcatcagcgt gaatcgacac gaggaaattg gcgttttgct tatgtgccac
    12961 atcactgcgc cccatcaccg agataaagta atccccgtca cgggttaaaa cgcctttaaa
    13021 catcggatcg tcattgagca aagtacgcaa tttacgcgcg atggcgatgg tgacattttt
    13081 ctcccgcgta ccaccggggc cgatagcgcc agggtcctga ccgccgtgtc cggcatcaat
    13141 agcgatgata attttatcgc cagtgttagc cgtcgcgcgc gctgccggac gcgttaccgt
    13201 attactgctg ataacacccg tagtgcggtt actttccgtt ttaaacggat tgcgcgccgg
    13261 ttcgctgacg cgcggtgcga caaccgcagg cgtttcaacg cgtttcgcaa ccacgggcgg
    13321 cggaggaggc ggtggcggca catcggcgtt aatcgtaaag acgacagtgt aattgctgcc
    13381 attctgccgc ttcaccgctt cggttttacc gttttcggta agatcgacca ccagccgtag
    13441 cgtttgtgca tctttaggcg ttccagagcg aatcgccttc accagattat tgccgctgaa
    13501 caacaacggc agtccctgaa tcatgcccgt ttgtttgata tcgagcgcca cggtgcgttt
    13561 gctttgatgg ctaaacgcat aatcaggatc gccaataaaa ctcaacgtta tccgcgcctg
    13621 ttgattaccg ttagaaacct gaatatcaga gagcgtcgcg gcacccaccg gcgtgcacag
    13681 cagcagcagc gtcgctacca accaatttct gatgcgatac atcatcccgc cacctttcaa
    13741 aggttaaccg gctaaacgcg ccagcaacaa ttcacccgca gaggaaaccg cactcacgcg
    13801 cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg acatccgggt caggaagaac
    13861 acctgtacct tgttgtggcc actccaccag gcagatggca tcgttggcaa aataatcgcg
    13921 gatccccata aactccagct cctcgggatc ggcaaggcgg tacaaatcaa agtgatagac
    13981 cattaagttg tcgagcgtat agggttcgac cagcgtataa gtggggcttt tgacattacc
    14041 ctgatgaccc agagcctgta aaaagccccg gctaaaggtg gttttacctg cgcctaaatc
    14101 gccatacaga tagattacgg ttgcgccatc gcaggctttc gctacccgct cgcccaggtc
    14161 taatgttgcc tgctcatcag ggagcggaat tactcgattc atcatggttt ttatcagtca
    14221 cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc ggttgccagc atcccgcgcg
    14281 ttccaaaacg cgccgccagt acgtcagctg ccgcaccgtg cgcgacacag cctgcacagg
    14341 ctgcatcata cggcgacagt ttttgcccaa gcaatgcgcc aataatacca gagagcacat
    14401 cgcccatgcc gccgctcgcc atgcctgcat ttccggcatc aataatgcct aaagcgtcag
    14461 gatgggcggc gaccacggtt ccggcacctt tcagcaccgc tacgccgcca taacgttgta
    14521 ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc agcgacggaa cagcctaaca
    14581 accgtgcggc ctcgctagga tgcggcgtga tcacgcgatt gtgacgctta tcgggattga
    14641 ttgccagcag gttcaatgca tcggcatccc acaacatcgg tttgcgaaaa ttctcaactt
    14701 tttgcagggc ttttttcccc cactcttgct ggcccagacc gggaccaatc accaccacat
    14761 cggcccattc caggctttcg gtaagagagt ccatagtcag ttcatgcacc atcaattctg
    14821 gtcgtgcagt cagcagcggc gcaatgttct cactgcgggt cagtactcgg actaaaccag
    14881 caccagcacg tagcgccgct tcccccgtca tacgaatagc ccccgccgtg ccgtgatcgc
    14941 caccgataat caccagccgc ccgtgatcgc ctttatgcga agtcgggcga cgcggtttca
    15001 gccagtgaga aagttgttct gccgaaaacc gctgaatttt cgtctcctga cctgccagcc
    15061 agctatccag ccccagtgag tcaaaatgca gttgtcctgt aacatcccgc gcttttccag
    15121 tgagcaagcc tagtttcagc gcaataaaag tgatggtgtg atcggcgttg atcactgcgc
    15181 ctggcgtagc gccggtttca gccagcaggc cggaagggat atcaaccgcc acaatcggcg
    15241 caggatggga attagcgtgg tcgattaact ggctaatgga ttcgcggggc gcttgctgca
    15301 agccggtacc gagcagcgca tcaacaatca gatctaccga ttcgggccag acaatattcg
    15361 aagcatggat ctcgccaccc gcgtttaacc atgcttcgcg tgccagcgcg gcctcttccg
    15421 gcaacggttt gtcgctctcc tgggccaaca acgtgacctc aatgccgacc gctttggcca
    15481 gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg accgcacagc accagccagt
    15541 ggcgggcgtc aggatacgcc gaacgacaca cctggaatgc ggcctcgcca gcgcgaagca
    15601 tcagctcata gagtgtgagc cccagcacat ctgccgcctc gcgttctccg cggcggatat
    15661 cgtcggcgta ccagacggtg tgtggtatac ttacggggtt tttcttcatt gtatggtccg
    15721 tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa aattaaacag tgggggctgg
    15781 aactgggctt tcagcaggta ggtattaccg ataccgatct cagcgagtcc gagcccaaac
    15841 tgcaagcatg gctggacaaa caataccacg gcgaaatgga ctggatggca cgtcacggta
    15901 tgctgcgcgc tcgcccccat gagttattac ccggtacgct gcgtgtaatc agcgtgcgga
    15961 tgaattacct tcctgctaac gccgcatttg ccagcacgct gaaaaacccc aaactcggct
    16021 atgttagccg ttatgcgctg ggccgtgact atcacaaact tctgcgcaac cgactcaaaa
    16081 agctgggcga gatgattcag caacattgtg tttcgctgaa ttttagaccg tttgtcgatt
    16141 ctgcgcctat tctcgagcgc ccgttagctg aaaaagctgg gctcggctgg acaggtaagc
    16201 actcacttat cctcaatcgc gaggccggtt cgttcttctt tttaggcgaa ttgctggtcg
    16261 atattccgct gcccgtggat caaccagtcg aggaaggatg cggcaaatgc gtggcctgta
    16321 tgacgatttg cccgaccggt gccatcgtcg agccatatac cgtcgatgct cgccgctgta
    16381 tctcttatct caccatcgaa cttgaagggg cgctcccgga agagttgcga ccgttaatgg
    16441 gaaaccgtat ttacggttgc gatgactgcc agcttatctg cccgtggaat cgctattcgc
    16501 aactcactac agaagacgat ttcagcccgc gtaagccgct acacgcaccg gaactcattg
    16561 agttatttgc ctggagcgaa gagaagtttt taaaagtcac ggaaggatcg gcgattcgcc
    16621 gtataggtca cctgcgctgg ctgcgtaata tcgccgtggc attaggcaat gccccttggg
    16681 atgaaacgat tttgacagcg ctggaaagtc gtaaaggtga gcacccactt ctcgatgagc
    16741 acatagcgtg ggcgattgcg cagcaaatag agagacgaaa tgcgtgcata gtcgaagtgc
    16801 aattgccgaa aaaacagcgt ctggttcggg tgattgaaaa agggttaccg cgtgacgcct
    16861 gattcatcca cagcttgtga ataaaaataa aaacgcattg caattcaaga cgaataaaat
    16921 cgtcaagtga tcgactcgac aaaacggaat cttgattttt aacttattta tcaataggtt
    16981 aaaaaaatac cattcaccgt gcgaagtttc tttgaaatta agattacagc ctgaggctgt
    17041 ggataactct gtttacaaga gtttgtcaga gacaacaaaa accagcaata acgcatcgcg
    17101 tcgctgtaga tattttattg agagaaga
//
LOCUS       DAJVQR010000084        17079 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVQR010000084 DAJVQR010000000
VERSION     DAJVQR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..508
                     /locus_tag="OQM52_002982"
     CDS             <1..508
                     /locus_tag="OQM52_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCS1564749.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OQM52_002983"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OQM52_002983"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCS1564750.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="OQM52_002984"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="OQM52_002984"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS1564751.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="OQM52_002985"
     CDS             complement(2876..3073)
                     /locus_tag="OQM52_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1564752.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OQM52_002986"
     CDS             complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OQM52_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS1564753.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OQM52_002987"
     CDS             complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OQM52_002987"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS1564754.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OQM52_002988"
     CDS             complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OQM52_002988"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS1564755.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OQM52_002989"
     CDS             complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OQM52_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS1564756.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OQM52_002990"
     CDS             complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OQM52_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS1564757.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OQM52_002991"
                     /pseudo
     CDS             complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OQM52_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10661..11617
                     /gene="fepB"
                     /locus_tag="OQM52_002992"
     CDS             10661..11617
                     /gene="fepB"
                     /locus_tag="OQM52_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1564758.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OQM52_002993"
     CDS             complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OQM52_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS1564759.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12964..13968
                     /gene="fepD"
                     /locus_tag="OQM52_002994"
     CDS             12964..13968
                     /gene="fepD"
                     /locus_tag="OQM52_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS1564760.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13965..14957
                     /gene="fepG"
                     /locus_tag="OQM52_002995"
     CDS             13965..14957
                     /gene="fepG"
                     /locus_tag="OQM52_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS1564761.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14954..15769
                     /gene="fepC"
                     /locus_tag="OQM52_002996"
     CDS             14954..15769
                     /gene="fepC"
                     /locus_tag="OQM52_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1564762.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OQM52_002997"
                     /pseudo
     CDS             complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OQM52_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3991 a         4862 c         4442 g         3784 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
     4561 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
     4621 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
     4681 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
     4741 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
     4801 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
     4861 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
     4921 agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg
     4981 gcgagaacca ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc
     5041 agcaccggcc ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc
     5101 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
     5161 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
     5221 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
     5281 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
     5341 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
     5401 tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa
     5461 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
     5521 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
     5581 cgccgcccct gttcatcgaa aacgacgatt tcccaactct gattttgcct cccaagatgc
     5641 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
     5701 agttccgtgc ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
     5761 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
     5821 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagaagggt atacacaatt
     5881 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
     5941 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
     6001 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
     6061 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
     6121 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
     6181 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
     6241 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt
     6301 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
     6361 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
     6421 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
     6481 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
     6541 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
     6601 aacctgtaac tttcgctccc gcctcaacaa acgtcagcgc cgtggcgtag ccgatacctt
     6661 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
     6721 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
     6781 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
     6841 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac
     6901 ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac
     6961 cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
     7021 atcagccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
     7081 ggtggtcata cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact
     7141 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
     7201 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg
     7261 ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
     7321 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
     7381 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
     7441 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt
     7501 attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
     7561 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
     7621 cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt
     7681 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct
     7741 cttttaccac cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca
     7801 gtgcggcata gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga
     7861 tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct
     7921 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
     7981 cattgtgctg cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc
     8041 gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt
     8101 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg
     8161 tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag
     8221 cgggaatacg cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca
     8281 gcgaggcaag ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg
     8341 ccggcggcac cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg
     8401 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
     8461 ctggcgaact catagtgtag ttatgagccg ccgggatcga gcacaggtag cgcgtctgtt
     8521 gtgtgaactg acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc
     8581 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
     8641 cagcaggtga tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt
     8701 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa
     8761 atgtgttgag gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg
     8821 ccggttcaat ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca
     8881 gcaccggcgc gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt
     8941 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg
     9001 cgaggttatc cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa
     9061 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc
     9121 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
     9181 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
     9241 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
     9301 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
     9361 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag
     9421 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca
     9481 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
     9541 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
     9601 tgcgttcgcg cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt
     9661 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
     9721 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
     9781 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
     9841 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
     9901 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
     9961 cggcgcgggc acccatctgt tcaaacgtgg tttgttcagg aattgcctag cgttccacca
    10021 cgttcagcga ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga
    10081 acgactgcta ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    10141 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    10201 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg
    10261 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    10321 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    10381 caaaatgata aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag
    10441 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg
    10501 ttgtggttac aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg
    10561 tcctgcgccg ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt
    10621 cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta
    10681 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc
    10741 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    10801 gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt
    10861 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg
    10921 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    10981 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    11041 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    11101 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    11161 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    11221 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    11281 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    11341 gaaattgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    11401 gcttggtggg gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg
    11461 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt
    11521 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    11581 catgcaagtg ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    11641 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    11701 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    11761 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    11821 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    11881 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    11941 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    12001 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    12061 cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    12121 cagccagcgc cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca
    12181 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg
    12241 gctgcggtgg cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc
    12301 ccgccgccgc cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa
    12361 tcatgggcga aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca
    12421 ttaagttttc acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg
    12481 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    12541 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    12601 tcactttttt gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    12661 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct
    12721 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    12781 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    12841 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    12901 acatgttaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    12961 cgtatatctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    13021 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    13081 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    13141 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    13201 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    13261 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa
    13321 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    13381 agtcagggtg gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    13441 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    13501 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    13561 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    13621 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    13681 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    13741 gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    13801 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    13861 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    13921 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    13981 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    14041 gattacgcag cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg
    14101 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    14161 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    14221 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    14281 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    14341 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    14401 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    14461 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    14521 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    14581 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    14641 cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    14701 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    14761 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    14821 cgctgttact gctggcggcc gttctctgcg cccaacaact gtttatgccg tatcaacttc
    14881 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    14941 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    15001 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    15061 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    15121 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    15181 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    15241 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    15301 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    15361 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    15421 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    15481 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    15541 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    15601 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    15661 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc
    15721 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    15781 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    15841 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    15901 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    15961 gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    16021 ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    16081 gagggaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    16141 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    16201 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    16261 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    16321 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    16381 cgtccaggat gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc
    16441 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    16501 ctcttttaat tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    16561 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    16621 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    16681 gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc
    16741 tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc
    16801 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa
    16861 atgagcgtca cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag
    16921 tccaataggg gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatccgaagc
    16981 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca
    17041 tcaaacctat aaatcaaaca aataaaaaac ccataatta
//
LOCUS       DAJVQR010000085        16397 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVQR010000085 DAJVQR010000000
VERSION     DAJVQR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(90..2066)
                     /gene="speA"
                     /locus_tag="OQM52_002998"
     CDS             complement(90..2066)
                     /gene="speA"
                     /locus_tag="OQM52_002998"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1564763.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="OQM52_002999"
     CDS             complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="OQM52_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1564764.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2342..2557
                     /locus_tag="OQM52_003000"
     CDS             2342..2557
                     /locus_tag="OQM52_003000"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564765.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2861..4015
                     /gene="metK"
                     /locus_tag="OQM52_003001"
     CDS             2861..4015
                     /gene="metK"
                     /locus_tag="OQM52_003001"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1564766.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4456..5850
                     /gene="galP"
                     /locus_tag="OQM52_003002"
     CDS             4456..5850
                     /gene="galP"
                     /locus_tag="OQM52_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1564767.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5927..6424
                     /locus_tag="OQM52_003003"
     CDS             5927..6424
                     /locus_tag="OQM52_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1564768.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6519..7226
                     /gene="endA"
                     /locus_tag="OQM52_003004"
     CDS             6519..7226
                     /gene="endA"
                     /locus_tag="OQM52_003004"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1564769.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7306..8037
                     /gene="rsmE"
                     /locus_tag="OQM52_003005"
     CDS             7306..8037
                     /gene="rsmE"
                     /locus_tag="OQM52_003005"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1564770.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8050..9000
                     /gene="gshB"
                     /locus_tag="OQM52_003006"
     CDS             8050..9000
                     /gene="gshB"
                     /locus_tag="OQM52_003006"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1564771.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9109..9672
                     /locus_tag="OQM52_003007"
     CDS             9109..9672
                     /locus_tag="OQM52_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1564772.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9672..10088
                     /gene="ruvX"
                     /locus_tag="OQM52_003008"
     CDS             9672..10088
                     /gene="ruvX"
                     /locus_tag="OQM52_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1564773.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10264..11244)
                     /locus_tag="OQM52_003009"
     CDS             complement(10264..11244)
                     /locus_tag="OQM52_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1564774.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11262..11966
                     /gene="yggS"
                     /locus_tag="OQM52_003010"
     CDS             11262..11966
                     /gene="yggS"
                     /locus_tag="OQM52_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1564775.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11984..12550
                     /gene="yggT"
                     /locus_tag="OQM52_003011"
     CDS             11984..12550
                     /gene="yggT"
                     /locus_tag="OQM52_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1564776.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12547..12837
                     /locus_tag="OQM52_003012"
     CDS             12547..12837
                     /locus_tag="OQM52_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1564777.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12845..13438
                     /locus_tag="OQM52_003013"
     CDS             12845..13438
                     /locus_tag="OQM52_003013"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1564778.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13431..14567
                     /gene="hemW"
                     /locus_tag="OQM52_003014"
     CDS             13431..14567
                     /gene="hemW"
                     /locus_tag="OQM52_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1564779.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14881..15844
                     /locus_tag="OQM52_003015"
                     /pseudo
     CDS             14881..15844
                     /locus_tag="OQM52_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15889..16392
                     /locus_tag="OQM52_003016"
     CDS             15889..16392
                     /locus_tag="OQM52_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCS1564780.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4064 a         4222 c         4123 g         3988 t
ORIGIN      
        1 aaaagcagct agattatttt taagaatata taaaaagaaa gcgggccgat ttacgtaaat
       61 aaatcgaccc gccagcggaa taatcttaat tattcatctt caagataagt ataaccgtac
      121 aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc
      181 ttcacttgat cgcggaactg ggttaacagc gttttcggat cgagctgtac atattgcagc
      241 atgtccgcca cggtatcgcc ttcgtcagac agttctactt ctacgctacc gtcagggaac
      301 acgaacacgt caactgcttc ggtatcaccg aacaggttgt gcatattgcc aaggatctcc
      361 tgatatgcac cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc
      421 attggcattg tcgtggcaat accatcacca tcaatatagt ggtcgatagc accgtcagag
      481 tcacaggtaa tatccagcag cacagcgcga cgttccggca cttgatccag cccttccagc
      541 ggtagaaccg ggaacaactg gtcgatcccc catgcgtccg gcattgactg gaacagcgag
      601 aaattgacgt acattttgtc cgccatacgt tcctgcagtt cgtcgattat cggacgatgc
      661 gcacggtttt gcggatccag ttgcttctgc acttcatggc acatgctcag atagagttgt
      721 tctgcccatg cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg
      781 tgcagatcca tctggctgtc gtgtaaccat tcacgcaaag aacggcgagt tcccggttcg
      841 tgcatttcct gccaggtttc ccacatgctt tgcagcgcac gcggcgcatc ttctacgggc
      901 gcggtcggca ccgtatattc gttacgttcc acgccgatga tattcgacac cagcacggta
      961 tggtgcgcag tcaccgcacg gcccgattcg gtgattaccg tcggatgcgg cagaccattt
     1021 tcttcacacg catcgccaat cgcccagatg atgttgttgg cgtattcatt gaggccatag
     1081 tttacggagc agtcagactg cgaacgggtg ccttcataat ccacgcccag accgccgccg
     1141 acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat
     1201 tcacgaacgc ctgtcgcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc
     1261 agtagttgca ggctgtcgag acgcccggct tcacgcaggg tttcaaccag ttgcagtacc
     1321 tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aggactgcca tttacccgag
     1381 ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct
     1441 tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc
     1501 atcttctcgc caattaatgc caggcggata tattcgcggt ctttataacc gttgcagacg
     1561 atgacgctac gagtcatgcc agcatgtgcc agcactgcca tcaactcggc tttggaaccg
     1621 gcttccagac ccagcggttc acccgaatga atcagggact cgatcacgcg gcggtgctgg
     1681 ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga ttccctcgca
     1741 cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac
     1801 agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg
     1861 cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca
     1921 tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg
     1981 gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc
     2041 aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac
     2101 agttaacgac tgtcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca
     2161 gcagtgtatg ctgacgctcc gactgcgcaa ccggtttctt tttcataaca ttattaagca
     2221 cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa
     2281 gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga
     2341 gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgagtctt
     2401 caccagaacg gtgagacagg ttaagcagca gacaacggtt cattatttcg tatcacctcc
     2461 acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt
     2521 ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc
     2581 cacaatggca gaaaattgca aaagataaat acgcagaatg ccggcattgt caggaaaaat
     2641 ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa
     2701 gaattgacct aaaatagcca tccagatgtt aatccatcca taccgattaa cactcagact
     2761 gccagtgttt ttaacctgca gagtcgtggt aggatccgct accacagaaa atccacacaa
     2821 cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac
     2881 gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt
     2941 tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa
     3001 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga
     3061 gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc
     3121 taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca accaaggcgt
     3181 tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc
     3241 aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca
     3301 gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag
     3361 ccaggtgact ttccagtatg acgacggcaa aatcgttggt attgatgctg tcgtgctttc
     3421 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat
     3481 catcaagcca attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc
     3541 gaccggtcgt ttcgttatcg gtggcccaat gggtgactgc ggtctgactg gccgtaaaat
     3601 tatcgttgat acctacggcg gcatggcgcg tcacggtggc ggtgcattct ctggtaaaga
     3661 tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt tatgtcgcga aaaacatcgt
     3721 tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga
     3781 accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg aacaactgac
     3841 tctgctggta cgtgagttct tcgacctgcg cccatacgga ctgattcaga tgctggatct
     3901 gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc
     3961 gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt
     4021 cttcacctgc gttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg
     4081 gataggcgtt cacgccgcat ccggcaaaaa aactgcccgc acaataacat cattcttcct
     4141 gatcacgttt cacggcaaat taccatcaca actgaaaccg attacaccaa ccacaccagc
     4201 aaaatatttg taatattttc atattattat tcagctttca cagttgttac atttcttttc
     4261 agtaaagtct tacatcattg cagatgacag cgtttaatct atgatgatat aactcaatta
     4321 ttttcatgcc cttaaatcat aactaagata aatgttagtg taagcgatta cactgatgtg
     4381 atttgcttca catcttttta cgtcgtactc acctatctta attcacaata aaaaataact
     4441 atattggagg gcatcatgcc tgacgctaaa aaacaggggc ggtcaaacaa ggcaatgacg
     4501 tttttcgtct gcttccttgc cgctctggcg ggattactct ttggcctgga tatcggtgta
     4561 attgctggcg cactgccgtt tattgcagat gaattccaga taacttcgca cacgcaagaa
     4621 tgggtcgtaa gctccatgat gttcggtgcg gcagtcggtg cggtgggcat cggctggctc
     4681 tcctttaaac tcgggcgcaa aaagagcctg atgatcggcg caatcctgtt tgttgccggt
     4741 tcactgttct ctgcggctgc gccaaacgtt gaagtactga ttctttcgcg cgttctgctg
     4801 gggctggcgg tgggtgtggc ctcttatacc gcaccactgt acctgtcaga aattgcgccg
     4861 gaaaaaattc gcggcagtat gatctcgatg tatcagttga tgatcactat cgggatcctc
     4921 ggtgcttatc tttctgatac cgccttcagc tacaccggtg catggcgctg gatgctgggt
     4981 gtgattatca tcccggcaat tttgctgctg attggtgtct tcttcctgcc agacagccca
     5041 cgttggtttg ccgccaaacg ccgttttgtt gatgccgaac gcgtgctgct acgcctgcgt
     5101 gacaccagcg cggaagcgaa acgcgaactg gatgaaatcc gtgaaagttt gcaggttaaa
     5161 cagagtggct gggcgctgtt taaagagaat agcaacttcc gccgcgcggt gttccttggc
     5221 gtactgttac aggtaatgca gcaattcacc gggatgaacg tcatcatgta ttacgcgccg
     5281 aaaatcttcg aactggcggg ttataccaac accaccgagc aaatgtgggg gacagtgatt
     5341 gtcggcctga ccaacgtact tgccaccttt atcgcaatcg gccttgttga ccgctgggga
     5401 cgtaaaccaa cgctaacgct gggcttcctg gtgatggctg ctggcatggg cgtactcggt
     5461 acaatgatgc atatcggtat ccactctccg tcggcgcagt atttcgccat cgccatgctg
     5521 ctgatgttta ttgtcggttt tgccatgagt gccggtccgc tgatttgggt actgtgctcc
     5581 gaaattcagc cgctgaaagg ccgcgatttt ggcatcacct gctccaccgc caccaactgg
     5641 attgccaaca tgatcgttgg cgcaacgttc ctgaccatgc tcaacacgct gggtaacgcc
     5701 aacaccttct gggtgtatgc ggctctgaac gtactgttta tcctgctgac attgtggctg
     5761 gtaccggaaa ccaaacacgt ttcgctggaa catattgaac gtaatctgat gaaaggtcgt
     5821 aaactgcgcg aaatcggcgc tcacgattaa tctcctcagg cttcctcccg tcgcggagga
     5881 agccgcctct tgcagtcatc ttttcttcgc tctatcctct gccgctatga aaacatcccg
     5941 tctccctatc gccatccaac aggccgttat gcgccgcctg cgggaaaaac tcgcccaggc
     6001 caacctgaag ctagggcgta actacccgga gccaaaactc tcttacaccc agcgcggaac
     6061 ctccgccgga acagcctggc tggaaagcta tgaaatccgc ctcaatcccg ttttgctgtt
     6121 ggaaaacagt gaagctttta ttgaagaagt ggtaccgcac gaactggcac atttgctggt
     6181 atggaaacat ttcggccgcg tagcgccaca tggcaaagag tggaagtgga tgatggaaag
     6241 cgtgctgggc gttcccgccc gtcgtacgca tcagttcgaa ctgcaatccg tgcgtcgcaa
     6301 caccttcccc taccgctgta agtgccagga acatcagctt accgtacgcc gccataatcg
     6361 cgtagttcgt ggcgaggcca tctatcgctg tgttcactgc ggtgaacagc tggttgcgaa
     6421 ataaccacct gaactatcag gaactttcct gatctggctg attgcatacc aaaacaactt
     6481 tcgctacgtt gctggctcgt tttaacacgg agtaagtgat gtaccgttat ttgtctattg
     6541 ctgcggtggt actgagcgca gcattttccg gcccgacgtt ggccgaaggt atcaatagtt
     6601 tttctcaggc gaaagccgcg gcggtaaaag tccacgctga tgcgcccggt acgttttatt
     6661 gcggatgtaa aattaactgg cagggcaaaa aaggcgttgt tgatctgcaa tcgtgcggct
     6721 atcaggtgcg caaaaatgaa aaccgcgcca gccgcgtaga gtgggaacac gtcgttcccg
     6781 cctggcaatt cggtcaccag cgccagtgct ggcaggacgg tggacgtaaa aactgcgcta
     6841 aagatccggt ctatcgcaag atggaaagcg atatgcataa cctgcagccg tcagtcggcg
     6901 aggtgaatgg cgatcgcggc aattttatgt acagccagtg gaatggcggc gaaggccagt
     6961 acggtcaatg cgccatgaag gtcgatttca aagaaaaagc tgccgaacca ccagcgcgtg
     7021 cacgcggtgc cattgcgcgc acctacttct atatgcgcga ccaatacaac ctgacactct
     7081 ctcgccagca aacgcagctg ttcaacgcat ggaacaagat gtatccggtt accgactggg
     7141 agtgcgagcg cgatgaacgc atcgcgaagg tgcagggcaa tcataacccg tatgtgcaac
     7201 gcgcttgcca ggcgcgaaag agctaaccta cactagcggg attctttttg ttaaccccta
     7261 ccccacgcgt acaaccgcgt ggggagacga cgcggatttt taactatgcg tatcccccgc
     7321 atttatcatc ctgaaccact gaccagccat tctcacatcg cgctttgcga agatgccgcc
     7381 aaccatatcg ggcgcgtact gcgcatgggg ccggggcagg cgttgcaatt gtttgacggt
     7441 agcaaccagg tctttgacgc cgaaattacc agcgccagca aaaaaagcgt ggaagtgaag
     7501 gtgctggaag gccagatcga cgatcgcgaa tctccgctgc atattcacct cggtcaggtg
     7561 atgtcgcgtg gtgaaaaaat ggaatttact atccagaaat cgatcgaact cggtgtaagc
     7621 ctcattacgc cacttttttc tgagcgctgc ggcgttaaac tggatagtga acgtctgaac
     7681 aagaagcttc agcagtggca gaagattgca attgctgcct gtgagcagtg tggtcgtaac
     7741 cgggtgccgg aaatccgtcc agcgatggat ctggaagcct ggtgtgcaga gcaggatgaa
     7801 ggactgaaac tgaatcttca cccgcgcgcc agtaacagca tcaatacgtt gcctttaccg
     7861 gttgaacgcg tccgcctgct gattggcccg gaaggcggtt tatcggcaga tgaaattgcc
     7921 atgactgccc gctatcaatt tactgatatc ctgttgggac ctcgcgtttt gcgtacagag
     7981 acaactgcgc tcaccgccat taccgcgcta caagtacgat ttggcgattt gggctaacgg
     8041 agaagaataa tgatcaagct cggcatcgtg atggacccca tcgcaaacat caacatcaag
     8101 aaagattcca gtttcgctat gttgctggaa gcacagcgtc gtggttacga acttcactat
     8161 atggagatgg gcgatctgta tctgatcaat ggcgaagccc gcgcccatac ccgcacgctg
     8221 aacgtgaagc agaactacga agagtggttt tcgttcgtcg gtgaacagga tctgccgctg
     8281 gccgatctcg atgtgatcct gatgcgtaaa gacccgccgt ttgataccga gtttatctac
     8341 gcgacctata ttctggaacg tgccgaagag aaagggacgc tgatcgttaa caagccgcag
     8401 agcctgcgcg actgtaacga gaaactgttt accgcctggt tctctgactt aacgccagaa
     8461 acgctggtta cgcgcaataa agcgcagcta aaagcgttct gggagaaaca cagcgacatc
     8521 attcttaagc cgctggacgg tatgggcggc gcgtcgattt tccgcgtgaa agaaggcgat
     8581 ccaaacctcg gcgtgattgc cgaaaccctg actgagcatg gcacttgcta ctgcatggcg
     8641 caaaattacc tgccagccat taaagatggc gacaaacgcg tgctggtggt ggatggcgag
     8701 ccggtaccgt actgcctggc gcgtattccg caggggggcg aaacccgtgg caatctggct
     8761 gccggtggtc gcggtgaacc tcgtccgctg acggaaagtg actggaaaat cgcccgtcag
     8821 atcgggccga cgctgaaaga aaaagggctg atttttgttg gtctggatat catcggcgac
     8881 cgtctgactg aaattaacgt caccagccca acctgtattc gtgagattga agcagagttt
     8941 ccggtgtcga tcaccggaat gttaatggat gccatcgaag cacgtttaca gcagcagtaa
     9001 cccaccttag cgagaaggat ctcgttgaga ctctgagtga cagcgccctt ctttccacgc
     9061 atactgggcg ctgttgcttt tttgaaccag gaaacagaac ctctgacaat gaatttacag
     9121 catcactttc ttattgccat gcctgctctc caggatccga ttttccgtcg ttccgtggtc
     9181 tacatttgcg aacataatac caatggtgca atggggatca tcgtcaacaa gccgctggaa
     9241 aatctcaaaa ttgaagggat tctggaaaag ctgaagatca cgccggagcc gcgtgatgaa
     9301 tcaatccgtc tggataaacc ggttatgctc ggcggcccac tggctgaaga tcgcgggttt
     9361 attttgcata ctccgccctc caattttgct tccagcattc gcatttcaga caacacggtg
     9421 atgaccactt cccgcgatgt gctggaaacg ctcggcaccg ataaacaacc gtctgacgta
     9481 ttggtggctc tgggctatgc ctcctgggaa aaaggtcaac tggaacaaga aattctcgat
     9541 aacgcgtggc taacggcccc ggcggatctg aatattctgt tcaaaacgcc gattgccgac
     9601 cgctggcgcg aggcggcaaa actgattggt gtggatattc tcaccatgcc tggtgtggca
     9661 ggacacgcct gatgagtgga accttactcg ccttcgactt cggcaccaaa agcattggcg
     9721 tagcggtcgg ccaacgcatt accggcaccg ctcgcccttt gcctgcaatt aaagcgcagg
     9781 acggtacgcc ggactggaac ctcatcgaac gtttactgaa agagtggcag cctgacgaaa
     9841 tcatcgtcgg tttgccgctg aatatggacg gcaccgaaca gccgttaacc gccagagcgc
     9901 gcaaatttgc caaccgtatt catggtcgtt tcggtgttga agtaaagctc catgacgagc
     9961 gtcttagcac tgtggaagcc cgttccggtc tttttgaaca gggcggctat cgggcgctca
    10021 acaaaggcaa agttgactct gcctctgcgg ttattattct cgaaagctat ttcgagcagg
    10081 gatattaagg cgatctaaaa gcctggcgga tagtcgttac cgccatcttg ccggatgcgg
    10141 cgtgaacgcc ttatccagca cacatctggc agcggctgta ggtctgataa gacgcgttag
    10201 cgtcgcatca gacatttatt gcctttatag gccctataaa cttgcgcatc gggcatggca
    10261 acgtcacaaa cgcccttccc ccacccgctg ctgataactc tgctgaaacg ttaacatccc
    10321 caccttctgc ccggtttgaa taacatgcgg taactggtgg gttttccctt cgcgaatcaa
    10381 attccccacc gcgggagtgt taatcagtaa ttcaaacagc gccacgcgtc cttcctgttt
    10441 atccacttcc agcttttgtg acagcactgc ccgtaaacta cccgcaagtt gattacgtac
    10501 gggatctttt tcctgcgccg gaaatgaatc caccagtcgc tcaactgcct gcgcggcacc
    10561 acgtgtatgt aaggttgcca gcaccagatg cccggtttct gccgccgtca gcgccagacg
    10621 gattgtctca ctgtcacgta gctctccgag caaaatcaca tcaggatctt cccgcaatgc
    10681 agcccgcaac cccgatgcga acgtcataca gtgcaaacca atttcccgct gctggatcaa
    10741 acatcgctgg ctggcataga gatattccac aggatcttcc agcgtcagaa tatgcgcatc
    10801 ggcatgttga ttaagatagc caaccatcgc cgccagcgtg gtagatttgc cactccccgt
    10861 agcccccgtc accagaatca ggccattctc gctcttgagt aattccggca ataccggtgg
    10921 tgcaccaagc tgttcgagct gtggacagtg cgaaggtaac aaccgtaatg ccagagaaat
    10981 accttgccgt tgcgcgaacg cactgccacg caaccgctgg ttttccgcca gcgacacggc
    11041 aaaatccagc tggccatttt ccagcaatat tgcccgctga tcgtcatcca gccactcccg
    11101 cagtagctct tcgacgtccg gcgcatcaaa cggcgcagct tccattctgc cacgaatgcg
    11161 ccatcgtgcg ggccaggcgc tgcacaggtg tagatccgag acgttatgct ttacactaag
    11221 ggccacaatt tcttccatat tcatactaag atcctcggaa aatgaacgat attgcgcata
    11281 acctggcaca ggtccgggac aaaatctcag cggctgcaac gcgttgcggc cgttctccag
    11341 aagaaattac gctgcttgca gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca
    11401 ttgatgccgg gcagcgtcaa tttggtgaaa actacgttca ggaaggggta gataaaattc
    11461 gccactttca ggaactgggc gtaacaggat tagaatggca ttttattggc ccgttgcagt
    11521 ctaataaaag ccgcctagtg gcagagcatt tcgactggtg tcataccatc gaccgtttgc
    11581 gcattgctac ccgcctcaac gagcagcgcc cggcagaact tccccctctt aacgtactga
    11641 ttcaaattaa tattagtgat gaaaacagta agtccgggat tcaactggca gaactggacg
    11701 agctggcagc ggcggtcgct gaactaccgc gtttacgtct gcgcgggctg atggcaatcc
    11761 ctgcgcttga gtcagaatat gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat
    11821 ttgccggact gaaaacgcgc tacccgcata tcgacacgct ctctctggga atgtcggacg
    11881 atatggaagc cgccattgcg gcaggtagca cgatggttcg tatcggcact gcaatttttg
    11941 gtgcgcgtga ttactctaaa aaataaggaa ttaaaggaac gccatgaata cgttgacttt
    12001 cctgctttca acggtcattg agctgtatac aatggtgctg ttattacgca tctggatgca
    12061 gtgtgctcat tgtgattttt acaccccctt ctcacagttt gtagtgaagg tagcgcagcc
    12121 aattatcggg ccactgcgcc gcgttattcc ggcaatgggt cccattgaca gcgcctcgct
    12181 gctggttgcc tatattctca gttttatcaa agccattgtg ctgtttgaag tggtgacctt
    12241 cctgccaatc atctggattg ccggtttact gattctgctg aaaaccatcg gcttgctgat
    12301 tttctgggtc ctgctggtga tggcgattat gagctgggta agccagggtc gtagcccgat
    12361 tgaatacgtg ctgattcagc tggccgatcc gctgctgcgc ccgattcgcc gcttgctacc
    12421 ggcaatgggt gggattgatt tctcgccgat gatcctcgtt ctgctgctgt atgccatcaa
    12481 tatgggtgtc gcagaagtat tacaggcaac cggaaacatg ctgctgccgg ggctgtggat
    12541 ggcgttatga gtgccgtaac agttaatgat gacggtctgg ttttacggct ctatattcag
    12601 ccgaaagcca gccgtgattc tattgtcggt ttacatggcg acgaagttaa agtcgccatt
    12661 accgcgccgc cggttgacgg ccaggccaac agtcatctgg tgaagtttct cggtaagcaa
    12721 ttccgggttg ccaaaagcca ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa
    12781 attaaaatca ttaatccgca acaaatcccg ccagaaatcg cggcgttaat taattaggta
    12841 tcctatgcaa aaagttgtcc tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc
    12901 gctgcttagc gactttggtc ttgatatcgt ggcccaaaca gacctcggcg ttgattccgc
    12961 tgaagaaacc ggcctaacct ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa
    13021 agtgaccggt ttaccggcaa ttgccgacga ctctggtctg gcggtagatg cgctgggtgg
    13081 cgcgccgggt atttactccg cgcgttattc cggtgaagac gcgaccgatc aaaagaatct
    13141 gcaaaaactg ttggaaacac tgaaagacgt accggacgac caacgtcagg cgcgtttcca
    13201 ctgcgtgctg gtatatctgc gtcacgcgga agatccgact ccgctggtat gccacggcag
    13261 ctggccgggc gtgattactc gcgaaccagc gggcactggt ggctttggtt atgatccaat
    13321 cttcttcgta ccttccgaag ggaaaaccgc cgccgaactg acccgcgaag aaaagagcgc
    13381 catttcccac cgtggtcagg cgttgaaact gctgctggac gctttacgta atggttaaat
    13441 taccgccgct gagtctctac attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg
    13501 atttcaactc tcacgcgttg aaaggagaag tgccgcacga cgattatgtt cagcatctgc
    13561 tttgcgatct ggacaatgat gtggcttacg ctcagggccg tgaagtaaag acaatcttta
    13621 ttggcggtgg tacgccgagc ctgctttccg gcccggcgat gcaaacgctg ctagacggcg
    13681 tgcgtgcgcg tttgccgctg gcagcggatg cagaaattac tatggaagcg aacccaggca
    13741 cggtagaagc ggatcgcttt gtcgattatc agcgtgctgg tgtaaaccgc atctctattg
    13801 gtgtgcagag ttttagcgaa gaaaagctga aacgacttgg gcgtattcat ggcccgcaag
    13861 aagcgaaacg agcggcaaag ctggcgagcg gtttagggtt acgtagcttt aaccttgatt
    13921 tgatgcatgg actaccggat caatcactgg aagaggcgct tggcgatcta cgccaggcca
    13981 ttgaactgaa tccgccgcat ctttcctggt atcaactgac tatcgaacct aatacgctgt
    14041 ttggctcgcg accgccggtg ctgccggacg atgacgcgct gtgggatatt ttcgaacagg
    14101 ggcatcagtt attaaccgca gcgggttatc agcaatatga aacttccgct tacgccaaac
    14161 ccggttatca gtgccagcac aatctcaact actggcgctt tggtgactac atcggtattg
    14221 gctgcggcgc gcacggcaaa gtgaccttcc cggatgggcg cattctgcgt accactaaaa
    14281 cgcgtcatcc tcgtggtttt atgcaaggaa ggtatctgga aagccagcgt gatgtcgaag
    14341 ccgcagataa gccgtttgag ttctttatga atcgcttccg tctgctggag gccgcgccgc
    14401 gcgtggagtt tattgcgtat accgggcttt gcgaagatgt gattcgccca cagttagacg
    14461 aggcgattgc ccagggttat ctcaccgaat gtgcggatta ctggcagata acggaacatg
    14521 gtaaattatt cttaaactca cttcttgaat tattcctcgc tgagtaatca ttaatgcgcc
    14581 tgcgggcgca ttaatcactt cttttatccc cccgttaatt aacctcggca gaaatatatt
    14641 tcttactgaa taaatatcga cgacatttat tatatatatt tagtgaatat ttactaatca
    14701 ttcattgatt ataaatatgt acaacctggc gcacaaattt aatatttaat ttctgacttt
    14761 atttcaagtt gtgataaaca tagacaagtt gtcagacaga ttaaccaatt tgtcgcacaa
    14821 atcactgata agcacaatta gatatcaacc aatttccatc cttcaacagg gagttttttt
    14881 atgaaaatga ggtcatttac ccgctcatta gtctgcgcat ccttgctggc tctggttagc
    14941 actggcgtaa acgccgcaga aaaagtggcg ttaaagttag cacacaacct ggaaagaagt
    15001 catgttgtac atcagagttt tgaagagctg gcgaaagagg taaaacagct gtcaaaaggt
    15061 aatatggtga tccgtattta tcccagcagc cagatgggta atgcgcgaga aactatggaa
    15121 ttgttgcaaa atggcgcact ggatatgacc aaaggttccg ccagtgatct ggagtctttt
    15181 gataatattt atgctatcta taatttgccg ttcttattaa gttgtttttg gtgaagttgg
    15241 taaagaaatt atggactcaa caaaagacaa aggctttttt gctttatcag cttatgtagc
    15301 gggtactcgc agtttctatg cgaaaaaacc gattacaaag ccagaagatc ttaaaggact
    15361 gaaaattcgt gttcagccaa gtccgaccac catcaaaatg attgaactga tgggtggttc
    15421 acccactcca atttcatttg gcgaagttta tactgcgatg cagcaaggtg tagtagatgg
    15481 cgcagaaaat aacgtgcctt cctggatgca gactcgacat attgaaattg ccaaagtttt
    15541 ttctgaagat gaacacgcct caattcctga ctttttggtt atctccacca aaacatggaa
    15601 taaattaacc ccagaacagc aacaaatcct cgaaacagcg gcgaagaaat ccgaagctta
    15661 tcaacaaaaa ttgtgggaaa aaattgacgc tgacacacgt gctcaggcga aagctatggg
    15721 gggtgaaatt gtaaaagtgg ataaagcgcc attccgtaaa gccgtgcaac ctctgtttga
    15781 cgacttcaaa aaagatccaa aacaagctgc tttgttagag aaattcgaca acgctgctca
    15841 ataatattcc gggccagcct ctggccctga ttttccagga tgcttacaat gatatttaac
    15901 cgcctgaaac ttgcggtaga ccgcgtcatt gccacgtttt ctgtcgccgt aatgcttgct
    15961 ctggttgtat gcgtagtctg gcaagtattt agccgctacg tacttaacca gccaagtaca
    16021 ttaaccgatg agctggcacg ctttttgatg atctgggtag gattattagg tgctgcatat
    16081 accgtcggcg cgcagcgaca tctctctatt gatttatttg ctctcgccct gaataagcgc
    16141 aaacaattac ttcttagtat agtcattaac gttctcattt taggttttgc cggttcggtc
    16201 attgtgactg gtggcctgaa attaattgat aaaacactgg caacatcaca ggtatctgcc
    16261 gctatgcaaa taccgatggg atacgtatat atcattctgc cattaagtgg agtggtaatg
    16321 atgttttatg ccttgtgttt tattaatcaa agcattcaac aactgaaaca acctgcgcag
    16381 gaggccagtt aatggat
//
LOCUS       DAJVQR010000086        15891 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVQR010000086 DAJVQR010000000
VERSION     DAJVQR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            436..1494
                     /gene="citC"
                     /locus_tag="OQM52_003017"
     CDS             436..1494
                     /gene="citC"
                     /locus_tag="OQM52_003017"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1564781.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1509..1805
                     /gene="citD"
                     /locus_tag="OQM52_003018"
     CDS             1509..1805
                     /gene="citD"
                     /locus_tag="OQM52_003018"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1564782.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1802..2710
                     /gene="citE"
                     /locus_tag="OQM52_003019"
     CDS             1802..2710
                     /gene="citE"
                     /locus_tag="OQM52_003019"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1564783.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2721..4253
                     /gene="citF"
                     /locus_tag="OQM52_003020"
     CDS             2721..4253
                     /gene="citF"
                     /locus_tag="OQM52_003020"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1564784.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4257..4807
                     /gene="citX"
                     /locus_tag="OQM52_003021"
                     /pseudo
     CDS             4257..4807
                     /gene="citX"
                     /locus_tag="OQM52_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4782..5660
                     /gene="citG"
                     /locus_tag="OQM52_003022"
     CDS             4782..5660
                     /gene="citG"
                     /locus_tag="OQM52_003022"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS1564785.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5711..7173
                     /gene="citT"
                     /locus_tag="OQM52_003023"
                     /pseudo
     CDS             5711..7173
                     /gene="citT"
                     /locus_tag="OQM52_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7287..8093
                     /gene="rna"
                     /locus_tag="OQM52_003024"
     CDS             7287..8093
                     /gene="rna"
                     /locus_tag="OQM52_003024"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1564786.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8322..8732
                     /gene="rnk"
                     /locus_tag="OQM52_003025"
     CDS             8322..8732
                     /gene="rnk"
                     /locus_tag="OQM52_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1564787.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8963..10172)
                     /locus_tag="OQM52_003026"
                     /pseudo
     CDS             complement(8963..10172)
                     /locus_tag="OQM52_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            10393..10821
                     /gene="uspG"
                     /locus_tag="OQM52_003027"
     CDS             10393..10821
                     /gene="uspG"
                     /locus_tag="OQM52_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1564788.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10942..12507)
                     /gene="ahpF"
                     /locus_tag="OQM52_003028"
     CDS             complement(10942..12507)
                     /gene="ahpF"
                     /locus_tag="OQM52_003028"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1564789.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12752..13315)
                     /gene="ahpC"
                     /locus_tag="OQM52_003029"
     CDS             complement(12752..13315)
                     /gene="ahpC"
                     /locus_tag="OQM52_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1564790.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13687..14433
                     /gene="dsbG"
                     /locus_tag="OQM52_003030"
     CDS             13687..14433
                     /gene="dsbG"
                     /locus_tag="OQM52_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1564791.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14643..15545
                     /locus_tag="OQM52_003031"
     CDS             14643..15545
                     /locus_tag="OQM52_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1564792.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15692..15891
                     /locus_tag="OQM52_003032"
                     /pseudo
     CDS             15692..15891
                     /locus_tag="OQM52_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3945 a         4102 c         3844 g         4000 t
ORIGIN      
        1 ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt
       61 ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata
      121 catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa
      181 cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa
      241 aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc ttaggatgta
      301 tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa
      361 ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc
      421 aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat
      481 aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca
      541 gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaattgcc
      601 ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg actggcgctg
      661 acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca tctgtttatt
      721 tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac gctgacctgc
      781 gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg ctatgccgaa
      841 tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat gaacgccaat
      901 ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg cgactggttg
      961 catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg gctggatctg
     1021 gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc agaatacatc
     1081 atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat taaccattgt
     1141 tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct tggtgtaact
     1201 caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa ccaggatatg
     1261 cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt tgaaattgag
     1321 cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact gctggcgaaa
     1381 aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta tttgcagaac
     1441 ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc atgagaaaca
     1501 ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct ggggatgtga
     1561 tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat agcagcgttg
     1621 agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc cgctacaacg
     1681 tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt ttacatgcac
     1741 gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg gaggattgcc
     1801 aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca tgttgtttgt
     1861 gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg atgccctgat
     1921 gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc gcatggttta
     1981 tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg tcaacgcgct
     2041 ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg cggacgttgt
     2101 gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag agatcctgcg
     2161 tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg cgattgaatc
     2221 tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt tgatcggtat
     2281 cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc cggaaggaac
     2341 tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg gtattcaggc
     2401 gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag aagccgccca
     2461 catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga ttgatctgct
     2521 gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg tcgtagaagc
     2581 cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca agatggtgga
     2641 cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt ccggcatccg
     2701 cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga caagaacggg
     2761 tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac tcaccaaagc
     2821 aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg gaagaagcga
     2881 ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct ttccgtggcg
     2941 gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt aaaaacctga
     3001 ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac attcgccagg
     3061 gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa gagatctccc
     3121 gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg catctggtac
     3181 agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt gatgaattcg
     3241 gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat gcaatggttg
     3301 atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct tatccgcata
     3361 atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat cgcgttggcg
     3421 atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc gaactgctta
     3481 ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa ggtttctcca
     3541 tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa gacaaaatgc
     3601 gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg atggttgatc
     3661 tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac agccatgctg
     3721 cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag tacgctaact
     3781 ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc gcgctggaaa
     3841 ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg cgtggcgctt
     3901 ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc gcgccactgg
     3961 tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc ccgggctcca
     4021 gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt ccggaactag
     4081 cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg cgcgaacgtg
     4141 cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc gttgccgttg
     4201 tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa taagccatgc
     4261 acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg ctcgtcagcc
     4321 gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt ccactggtct
     4381 cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc cgaattttta
     4441 atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa attcaggagc
     4501 aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc gccccggctc
     4561 gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg cggttatggg
     4621 atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat tcactgcgcc
     4681 tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa aaacccatca
     4741 actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg cctgcaacgt
     4801 caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg ctgggctggc
     4861 gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat cgcattaact
     4921 gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg gcgattcagg
     4981 gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca ccggaagcgg
     5041 tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc cgcgccactg
     5101 cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt gcggcaattg
     5161 gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct acggcggcaa
     5221 gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga ctgacggcag
     5281 gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc gaagcaggtt
     5341 atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat caggggttag
     5401 atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac ggcgatacca
     5461 acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag gcgcaaacat
     5521 tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg cagttcgaca
     5581 gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg atccttacct
     5641 gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata ttaattttcg
     5701 gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc cactggtggt
     5761 gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg catggcatta
     5821 cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc cggcaacagc
     5881 gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc tctttgatgc
     5941 caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat ggggcctggc
     6001 tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg cattagggta
     6061 tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca tggacaaacg
     6121 cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac cgtttacacc
     6181 gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc tgccgccgtt
     6241 gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt tgatgtggat
     6301 gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg accaaacgtg
     6361 ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt gcagtggttc
     6421 ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct ttcctacgtg
     6481 ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg tgatgaacta
     6541 aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt attgctcagc
     6601 ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg tctgctggca
     6661 gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg ctataacagc
     6721 gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg cctgactcgt
     6781 tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg attctcacca
     6841 aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct gtttgccagc
     6901 ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa aggtattccg
     6961 ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat catgggctgt
     7021 ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta tgtgaaatca
     7081 aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat gttgctgttg
     7141 gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct gaataaatca
     7201 tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag atagtattca
     7261 gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc gcgttgctcg
     7321 cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca aaacagtatg
     7381 gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc cagagtcaac
     7441 acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg accaacaaaa
     7501 ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt gctgcccgtg
     7561 gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc ccgaatctac
     7621 cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca ctggaaacgg
     7681 ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg gaacgctacg
     7741 aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc ggtacgatgg
     7801 tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg gataattacg
     7861 gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg ggaaaagaga
     7921 acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact gaaattcaga
     7981 tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc ttgccacaac
     8041 ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat taatcctctg
     8101 atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt atacataatg
     8161 cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg agcaacagtc
     8221 agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct tttgggtatg
     8281 tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga ccaactatca
     8341 tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc gcctatgctg
     8401 gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg tgttcgccag
     8461 aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc aatcttagcg
     8521 atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat agcaatactc
     8581 agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt ggcgattcca
     8641 ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa ctcgaatacc
     8701 agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga taactcgctt
     8761 tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc ctggttctca
     8821 ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca tggcagaagc
     8881 attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg ctgtccttta
     8941 cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc accagacctg
     9001 aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc accttacggc
     9061 actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca aacggcatat
     9121 agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt aataattctc
     9181 ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta tcaaaggcgt
     9241 cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta acaatgccgc
     9301 aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc cgtggtgctg
     9361 cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg acctgccgtt
     9421 tgttcaataa ttgactgtgc cggatcgcta tcttcatcga aattaatcgg gatcgcgccg
     9481 tagcggtcag cggcgaaacg caagcggtag ggatgatgat caacaacaaa aatctgttcc
     9541 gcaccgagca accgtgcaca ggcgattgtc aacaatccca caggaccagc accatagact
     9601 gcaacgcttg aaccttgttg gatctgcaca ttttttgctg cctgccatgc cgttggcaga
     9661 atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt aaacggcccc
     9721 acattccctt tcgggacgcg gacatattcc gcctgcccgc caggaacgcc accatacagg
     9781 tgactataac caaacaatgc cgcgggagca ggtatctgtt ttttattgag cgcagcgcct
     9841 ttacccgcat tggtattttc gcaggcggca tattgctgta atcgacagaa aaaacagtcg
     9901 ccacaagcaa tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc
     9961 ttcccggttt caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc
    10021 tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt taccgtaata
    10081 cgcagaataa tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg
    10141 tgatgtgggc cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga
    10201 gtcagtcttt aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag
    10261 aattcctagt gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac
    10321 cagacaaata tcagggataa atattataat catggagaat gatttgttca tgattaacag
    10381 ggagaaaggg ttatgtataa ggcaatcatt atgccagttg atgtatttga aatggaattg
    10441 agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta
    10501 cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt
    10561 cgttttgaag agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac
    10621 ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa
    10681 gtcaatgagt tggcagaaga actgggggct gatgttgtag ttatcggttc tcgcaaccca
    10741 tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg
    10801 cctgtactgg tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc
    10861 ggcagggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt
    10921 gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact
    10981 cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt
    11041 acagtcaccc gctgcgaaca cgcctttgac gttggtttca catttcgcat caatgataat
    11101 ctcgcccatg cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc
    11161 aatctggaca aaaataccgg ccagttcgat gttgtgaata ttgccgctga cacgatctcg
    11221 atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag
    11281 aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat
    11341 ttctggcgca aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc
    11401 ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcgggcc
    11461 gtcgcagtgc gggcagtagg tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt
    11521 catgttgcgc cattttgcac ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc
    11581 agaagctgtt tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc
    11641 gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc
    11701 caacttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag
    11761 gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta
    11821 aattgctgcc gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg
    11881 cttgttcagc tcttctgccg cacgattttc cgcgccagta tcaattttgg caacgatttc
    11941 agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc
    12001 catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt
    12061 gatgcgcggg ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg
    12121 gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc
    12181 cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag
    12241 cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg
    12301 gttggtgatc aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac
    12361 tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct
    12421 gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag
    12481 ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca
    12541 agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt
    12601 gccgcagcac ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac
    12661 tagcctgtga atccccggga gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca
    12721 acgccgctat ggcgtgaaag acgaaggaaa tttagatttt accaaccagg tccagagacg
    12781 gagccagagt tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag
    12841 ctacgtactg agctgctttg attttacgca gcagatcaga cgcgtcacgg ccaatgcctt
    12901 cagcggtaac ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt
    12961 cagccagacc ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt
    13021 cgccgatcat cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt
    13081 gggtgaagtg agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt
    13141 cgtagtggtc agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt
    13201 agaagaagaa gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc
    13261 cgtttttgaa tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata
    13321 cttcctccgt gttttcgatg agatgtaagg taaccaattt ttgccgattc ggctaatgcg
    13381 tttccattat caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact
    13441 ttgcaccgcc agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct
    13501 gaactaccct ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg
    13561 caacaacgac atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc
    13621 ttacctatga cagtgatagg ttatgccttt tactcgactt ttgcactgac tgaaaagaac
    13681 aaattaatgt taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag
    13741 gaacttcctt ccccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc
    13801 gatgcccccg gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc
    13861 tacctgactc ccgatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa
    13921 aacctgagta acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg
    13981 caacggatgg aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc
    14041 tacgtcttct ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg
    14101 tgggtagatt ctggcaaagt gcaattaaga acattgttgg ttggggttat caagccagaa
    14161 agcccggcga cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa
    14221 tatgaagcct ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa
    14281 atgaaagtgt taagtgacaa tgagaaactg atggacgatc tgggggcaaa tgtcacgccg
    14341 gctatctatt acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag
    14401 aaaacgctta atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat
    14461 atccctgctt ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc taagtggctg
    14521 tgctatttta gaaaaactta atcctgagtt aagcaaaaaa acataatcaa taaaaataca
    14581 tacgcaaaaa acattgatta agtgaatata ccatggaaga aaaatataaa ccggagtagt
    14641 gtatggccaa tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt
    14701 gtatttacca gcatttaagc attagcaaag ctgctgaatc actatatatc accccatcag
    14761 ccgtgagcca gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta ttcattcgct
    14821 caggaaaagg tatcgcgccg acgacaacag ggttgaatct gcatcaccat cttgagaaaa
    14881 acctcaaagg ccttgaacaa acaattaata tagtcaataa gtccgaacta aagaaaaact
    14941 ttattatcta cggccctcag ctcatttctt gctctaataa tagtatgctg atccgctgcc
    15001 tacggcaaga tgcctctgtc gagattgagt gccatgatat cctcatgtca gcagagaatg
    15061 ctgaagaact gttggttcac cgcaaagccg atctggtgat cacccaaatg ccagtgatca
    15121 gccgttccgt catttgcatg ccattgcata caattcgcaa tactctaatt tgtagtaata
    15181 agcatccgcg aattactgac aatagcactt atgaacaaat tatggcagaa gagttcaccc
    15241 aattaatagc caaatcagca ggagttgatg atattcaaat ggaaatagac gagaagttta
    15301 tgaatcgcaa aatttcattt cgtggttcct ctttgctgac aataattaat agcattgcag
    15361 ttacagattt actgggaata gtcccctatg aactttataa ctaccaccgt gactttctta
    15421 atttaaaaga gataaaacta gaacatccgt taccttcaat aaagctttac atttcctata
    15481 ataaatcttc tttaaataat ttggtgttct cccgatttat tgaccgtcta aatgagagtt
    15541 tttgatatat aactatagac agctactata actttcatct atttattcac agcgcgaatg
    15601 aaaaataaaa cactctgctt tacagtgttt actttataat taacagtctt atctcactgt
    15661 tcaccctgta acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct
    15721 caatattctg gaagctgcac gagagcgtat tacctagaca ctcaacaccc taccccgcgt
    15781 ttgtgtctca ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact
    15841 tgcccgacaa atgggcaaaa aaatctgcgt tttgtttatc aactgggagg c
//
LOCUS       DAJVQR010000087        15526 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVQR010000087 DAJVQR010000000
VERSION     DAJVQR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OQM52_003033"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OQM52_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1564793.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OQM52_003034"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OQM52_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1564794.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OQM52_003035"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OQM52_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OQM52_003036"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OQM52_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1564795.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OQM52_003037"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OQM52_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1564796.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OQM52_003038"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OQM52_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1564797.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OQM52_003039"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OQM52_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1564798.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OQM52_003040"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OQM52_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1564799.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OQM52_003041"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OQM52_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1564800.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OQM52_003042"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OQM52_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1564801.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OQM52_003043"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OQM52_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1564802.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OQM52_003044"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OQM52_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1564803.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OQM52_003045"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OQM52_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1564804.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OQM52_003046"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OQM52_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1564805.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OQM52_003047"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OQM52_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1564806.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OQM52_003048"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OQM52_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1564807.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OQM52_003049"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OQM52_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1564808.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OQM52_003050"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OQM52_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1564809.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OQM52_003051"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OQM52_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1564810.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OQM52_003052"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OQM52_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1564811.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OQM52_003053"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OQM52_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1564812.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OQM52_003054"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OQM52_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1564813.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OQM52_003055"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OQM52_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1564814.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OQM52_003056"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OQM52_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1564815.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OQM52_003057"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OQM52_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1564816.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OQM52_003058"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OQM52_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1564817.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OQM52_003059"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OQM52_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1564818.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OQM52_003060"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OQM52_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1564819.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OQM52_003061"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OQM52_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1564820.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OQM52_003062"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OQM52_003062"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1564821.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OQM52_003063"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OQM52_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1564822.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3643 c         4130 g         3746 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJVQR010000088        15443 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVQR010000088 DAJVQR010000000
VERSION     DAJVQR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..73)
                     /locus_tag="OQM52_003064"
     CDS             complement(<1..73)
                     /locus_tag="OQM52_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCS1564823.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OQM52_003065"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OQM52_003065"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1564824.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OQM52_003066"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OQM52_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1564825.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OQM52_003067"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OQM52_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1564826.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OQM52_003068"
     CDS             2703..2939
                     /locus_tag="OQM52_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1564827.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OQM52_003069"
     CDS             2942..3799
                     /locus_tag="OQM52_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1564828.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OQM52_003070"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OQM52_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OQM52_003071"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OQM52_003071"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1564829.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OQM52_003072"
     CDS             complement(6414..7310)
                     /locus_tag="OQM52_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1564830.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OQM52_003073"
     CDS             complement(7359..7598)
                     /locus_tag="OQM52_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1564831.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OQM52_003074"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OQM52_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1564832.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="OQM52_003075"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="OQM52_003075"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS1564833.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="OQM52_003076"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="OQM52_003076"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1564834.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="OQM52_003077"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="OQM52_003077"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1564835.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..12879
                     /locus_tag="OQM52_003078"
     CDS             10765..12879
                     /locus_tag="OQM52_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1564836.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12889..14181
                     /locus_tag="OQM52_003079"
     CDS             12889..14181
                     /locus_tag="OQM52_003079"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564837.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15131..15223
                     /locus_tag="OQM52_003080"
     CDS             15131..15223
                     /locus_tag="OQM52_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCS1564838.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15267..>15443)
                     /locus_tag="OQM52_003081"
     CDS             complement(15267..>15443)
                     /locus_tag="OQM52_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1564839.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
BASE COUNT         3544 a         4083 c         4057 g         3759 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatca gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcg
//
LOCUS       DAJVQR010000089        15402 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVQR010000089 DAJVQR010000000
VERSION     DAJVQR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..1177)
                     /locus_tag="OQM52_003082"
     CDS             complement(<1..1177)
                     /locus_tag="OQM52_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1564840.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="OQM52_003083"
     tRNA            complement(1376..1460)
                     /locus_tag="OQM52_003083"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="OQM52_003084"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="OQM52_003084"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1564841.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="OQM52_003085"
     CDS             2804..4306
                     /locus_tag="OQM52_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1564842.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OQM52_003086"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OQM52_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1564843.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OQM52_003087"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OQM52_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1564844.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="OQM52_003088"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="OQM52_003088"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1564845.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="OQM52_003089"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="OQM52_003089"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1564846.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="OQM52_003090"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="OQM52_003090"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1564847.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENSGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="OQM52_003091"
     CDS             complement(11871..13067)
                     /locus_tag="OQM52_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS1564848.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="OQM52_003092"
     CDS             13260..13763
                     /locus_tag="OQM52_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1564849.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="OQM52_003093"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="OQM52_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1564850.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="OQM52_003094"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="OQM52_003094"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1564851.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEELGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3975 a         3931 c         3968 g         3528 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa cagtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agcttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa ta
//
LOCUS       DAJVQR010000090        15343 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVQR010000090 DAJVQR010000000
VERSION     DAJVQR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OQM52_003095"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OQM52_003095"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1564852.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OQM52_003096"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OQM52_003096"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1564853.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OQM52_003097"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OQM52_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCS1564854.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OQM52_003098"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OQM52_003098"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1564855.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OQM52_003099"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OQM52_003099"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1564856.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OQM52_003100"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OQM52_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1564857.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OQM52_003101"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OQM52_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCS1564858.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OQM52_003102"
     CDS             complement(8064..9206)
                     /locus_tag="OQM52_003102"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCS1564859.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OQM52_003103"
     CDS             complement(9215..10228)
                     /locus_tag="OQM52_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCS1564860.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OQM52_003104"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OQM52_003104"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1564861.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OQM52_003105"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OQM52_003105"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1564862.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OQM52_003106"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OQM52_003106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1564863.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OQM52_003107"
     CDS             complement(12826..13950)
                     /locus_tag="OQM52_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1564864.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OQM52_003108"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OQM52_003108"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1564865.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4380 a         3444 c         3145 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaa
//
LOCUS       DAJVQR010000091        14967 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVQR010000091 DAJVQR010000000
VERSION     DAJVQR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(509..1450)
                     /gene="fabY"
                     /locus_tag="OQM52_003109"
                     /gene_synonym="yiiD"
     CDS             complement(509..1450)
                     /gene="fabY"
                     /locus_tag="OQM52_003109"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1564866.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1495..1932)
                     /gene="dtd"
                     /locus_tag="OQM52_003110"
     CDS             complement(1495..1932)
                     /gene="dtd"
                     /locus_tag="OQM52_003110"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1564867.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1929..2801)
                     /locus_tag="OQM52_003111"
     CDS             complement(1929..2801)
                     /locus_tag="OQM52_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS1564868.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2795..3394)
                     /gene="yihX"
                     /locus_tag="OQM52_003112"
     CDS             complement(2795..3394)
                     /gene="yihX"
                     /locus_tag="OQM52_003112"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1564869.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3493..4278)
                     /locus_tag="OQM52_003113"
     CDS             complement(3493..4278)
                     /locus_tag="OQM52_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1564870.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4312..5208)
                     /gene="yihV"
                     /locus_tag="OQM52_003114"
     CDS             complement(4312..5208)
                     /gene="yihV"
                     /locus_tag="OQM52_003114"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCS1564871.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5376..6272
                     /gene="yihU"
                     /locus_tag="OQM52_003115"
     CDS             5376..6272
                     /gene="yihU"
                     /locus_tag="OQM52_003115"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1564872.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6296..7174
                     /gene="yihT"
                     /locus_tag="OQM52_003116"
     CDS             6296..7174
                     /gene="yihT"
                     /locus_tag="OQM52_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCS1564873.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7191..8432
                     /gene="yihS"
                     /locus_tag="OQM52_003117"
     CDS             7191..8432
                     /gene="yihS"
                     /locus_tag="OQM52_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCS1564874.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8570..9472
                     /locus_tag="OQM52_003118"
     CDS             8570..9472
                     /locus_tag="OQM52_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCS1564875.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9671..11707
                     /gene="yihQ"
                     /locus_tag="OQM52_003119"
     CDS             9671..11707
                     /gene="yihQ"
                     /locus_tag="OQM52_003119"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCS1564876.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11753..13127
                     /locus_tag="OQM52_003120"
                     /pseudo
     CDS             11753..13127
                     /locus_tag="OQM52_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13170..14573
                     /locus_tag="OQM52_003121"
     CDS             13170..14573
                     /locus_tag="OQM52_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1564877.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14641..>14967
                     /gene="ompL"
                     /locus_tag="OQM52_003122"
     CDS             14641..>14967
                     /gene="ompL"
                     /locus_tag="OQM52_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCS1564878.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3669 a         3855 c         3734 g         3709 t
ORIGIN      
        1 cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa cagaataact
       61 ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac ggtttaagag
      121 caaacataca acataatgat tcataacata cagagcagta aatgaatgat actgacaatg
      181 tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa aatatttatg
      241 aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac ctgaactaat
      301 atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata atatttaaat
      361 gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa aaatttataa
      421 agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg gaatgaatga
      481 gagaggcagc accgcccctc tccataagct actcttcttc gttcccgccc tcttcatacg
      541 ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca cccggcgtct
      601 cgtcgccaaa gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt
      661 cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg ctaatcggtt
      721 tgctgtagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg cgttcacgca
      781 gcatcagcca gataagcccc caaccggtga gcgtcgccag cgagaataaa ctcccggcaa
      841 acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata aatttttgcc
      901 cggtatattg ttgaatacgc acgcccattt tttcactaag cgggatatgt tcgtaccacg
      961 cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga gtggcgacgg
     1021 gcttaatcat caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt tctccctgat
     1081 taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc
     1141 gcttaacgcc ttcctgacgc gccaccgact ccagggtcat cgccatcagc gttcctaacc
     1201 ctttgtcctg cacgtccggg tgaacgacca taaagcgaat ggacgcttca ttgtcggcat
     1261 taatatacaa tcggcctacc gccaccagat taccctgctc gtcgacgacc atctggtgat
     1321 gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca
     1381 acatttccca gcgaaagtga tagtaacgct ctaactcttc ttctgtttgt ggaacctgaa
     1441 ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc tggctcatac
     1501 ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat cggcagcgaa
     1561 gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat agtcatataa
     1621 cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc cccgttcggt
     1681 atctgcggcg agggtaaact gggaaaccac cagtacactg ccgcccgcct gttgcacgtt
     1741 gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagcacac gctcgcacag
     1801 acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc
     1861 gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat
     1921 taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag tttgcggtat
     1981 tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt ccagtagacc
     2041 caaacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata tgacgggaac
     2101 atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc ggcgacaaac
     2161 gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct gtacagcaac
     2221 cagaaggaga tccacgacaa cagcaacgga aaaatacgca gcacgttgtc gatgacagta
     2281 ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag actggcccct
     2341 gccagcagcg gccccagcgt taaaatcatc cagtacacgg cgaacgagta aatttttggt
     2401 cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat
     2461 aacgcggtga cgatcagccc gcacgcccca acggcggtca tcttgttgga attagcaaca
     2521 aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt ggcaaaaata
     2581 aagtgacgta actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa
     2641 acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg
     2701 gttgtcatgt tgtcctcatc aatacgttgc cagagtagtt ttagccaggc ccatagtgga
     2761 cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt
     2821 ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga ttagctcctt
     2881 ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa ccttccgcct
     2941 gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct tgcgacagat
     3001 agatatggtc agcagcatca cgaatttccg ggtattcttc cggccagaag gtggtatgca
     3061 ggcggttggt attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga
     3121 tggcgatcac ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga gagaactgct
     3181 cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa
     3241 tttccccacg ctcatgctga tgaaacgcct cccccatatg aaaactcttc ttaagcgatg
     3301 ccagcggaat acgcgttaaa tcgctccagg ctcccagcac acggttaaag tcgatatcga
     3361 caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa
     3421 attaactgta gcgggaaagg atgggtttgg ctatgtggca aggtatgaag ccaggttaac
     3481 ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt tcaatttggc tgtcttgtaa
     3541 gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac
     3601 gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcagaat
     3661 atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgtttacat caaactccat
     3721 caacgcgcca tcgctctcaa tcgccccaac gcttgttacc agataatcag cacgaaaatc
     3781 agccacaaag gacgccgctg aagggccaat gatcccgcta ttatgagagc gcaacgtacc
     3841 gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct
     3901 gttggtgatt atccgcaaat gattatggtt aagtaacgcc cgggcaacat gctcaacagt
     3961 cgtaccaatg gtgataaata ttgttgaacc atcaggaata tagtctgcca ccgcttcggc
     4021 aatcgctttt tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg tattaacgac
     4081 gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct cgcttaattt
     4141 acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca gctcatcaat
     4201 attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg ggttaccggt
     4261 tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat tttatacaaa
     4321 aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc caccgggacg
     4381 tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta aatccccact
     4441 tgttgccagc gccaccgcca aagcgccgtg aaaaacatct cccgcacctg tggtatctac
     4501 cacatcaact ttgaaggccg gttgatgctg acgcccacca ttttccagcc agtcgcagcc
     4561 tgcgctaccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct gttttagcgc
     4621 actggccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa aggccgcgtg
     4681 atcgcttaat gccaccagct cactgatatc ctgcggcgta atgtccccgt ccagaacggt
     4741 catcacaccc gcctgacggg ccagggtgaa ggctttttta gcgccgtcgt gccagcgtac
     4801 atctgccagc acaacatccc actgagagaa atcaatttcc tccaaccact ctgcgtcagg
     4861 cagcagatcc gggctggggt agttaatgat tatccgctcg cctttggtat ccaccatgat
     4921 ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt taacgcccca
     4981 ggattccagc tctgccagca ggctgttgcc cgcgtcgtca tcacctacgc gaccaataaa
     5041 atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg gcccgccacc
     5101 aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaacc cttccacgta
     5161 atagatgcga tccatcacgg ttatacctac acaagcaaca cgaatcatgg tcattcctta
     5221 aacattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga cggctgtcaa
     5281 ttttttgacg ataaattaca aattcgatca aaaacagaca agcaaagcat gtcataaatg
     5341 acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg tttatcggtt
     5401 taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat caacttcgcg
     5461 tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg actcccgccg
     5521 ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg ccgaatggcg
     5581 atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta tctaccgatg
     5641 cgctggtcat tgatatgtcc accatccatc cgctgcaaac cgataaattg attgccgata
     5701 tgcaagccaa aggcttcagc atgatggatg ttccggtagg ccgtacttct gcaaatgcca
     5761 ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa cgtgccacac
     5821 cgatcctgat ggcgatgggc agtgagttga tcaacgctgg cggtccgggc atggggatcc
     5881 gcgttaagct catcaacaac tacatgagca tcgcgctcaa tgcgctttcg gcagaagccg
     5941 ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg atgagcggta
     6001 ccgccgccgg taaaggtcac ttcaccactt cctggccaaa caaagtcctc agcggagatc
     6061 tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc cttgatgtcg
     6121 ccaaccagct gcatgtgcca atgccgctgg gggccgcctc acgggaggtt tatagccagg
     6181 cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggaacag gtccgtgtca
     6241 gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata aatgaatgaa
     6301 taagtacacc atcaacgaca ttacgcgcgc atcgggcggt tttgccatgc tggcggtcga
     6361 tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg gcacccgctc cggtagccga
     6421 tagcgtttta actgatttca aagttaacgc tgcaaaggcc ctctcgcctt atgcctcggc
     6481 gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg cgattgccaa
     6541 aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta ttccggtcga
     6601 tagcgtggtt attgaccgca aaatcaatcc gctacagatc aaacaagacg gtggcaaagc
     6661 cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgtc tggatatggt
     6721 gaaagagttc aacgaactgt gccactcaca cggtctggta agcatcattg agccagtcgt
     6781 ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcgatcatcg atgccgccaa
     6841 agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt atggcaaagg
     6901 tccgcaacaa gagcttctct gtgcttcaca acgtctgaat gaccatatca atatgccatg
     6961 ggtgatcctc tcttccagtg tcgacgaaaa actgttcccg cgtgccgtac gcgtggcaat
     7021 gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc tgggcatcgg tcgtcggttt
     7081 accagacaac gagctgatgc tccgtgacgt ttgcgcaccg aaattacaac aacttggcga
     7141 tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag gataaaagga atgaaatggt
     7201 ttaacaccct aagccacaac cgttggctgg aacaggaaac cgaccgcatc tttgattttg
     7261 gtaaaaattc cgtagtgccg actggttttg gctggttagg caataaaggg caaatcaaag
     7321 aagagatggg cacccatctg tggatcacag ctcgtatgtt gcacgtttat tccgttgctg
     7381 cggcgatggg tcgacctggc gcttacgcgt tggttgatca cggtattaaa gccatgaacg
     7441 gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg cgtgaatgac gagggcgtgg
     7501 tggatgcctc caaacagggc tatcaacact tctttgctct gctgggtgcc gccagcgccg
     7561 tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta caccattgag attatcgaga
     7621 aatatttctg gagcgaagaa gagcagatgt gcctggagtc ctgggacgaa gccttcagca
     7681 aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc ggtggaagct ttcttgattg
     7741 tttatgacgt cactcacgac aaaaaatggc tggatcgcgc gattcgcgtg gcttccgtga
     7801 ttatccacga cgtcgccaga aataatcatt atcgcgttaa cgaacacttc gatacccagt
     7861 ggaatccgct gccggattac aacaaagata acccggcgca tcgcttccgc gcgttcggag
     7921 gtacaccagg ccactggatc gaatggggcc gtttaatgct gcacatccat gctgccctgg
     7981 aagcccgttg cgaacaacca ccagcatggc tgctagaaga tgccaaaggt ctgtttaacg
     8041 ccaccgtgcg cgatgcctgg gcacccgatg gagcggacgg gattgtttat accgttgact
     8101 gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc tatcgtcgaa gcaatgggta
     8161 cggtctacgc gctctacacc gtcactggcg atcgccagta tgaaacctgg tatcaaacat
     8221 ggtgggagta ctgcattaag tacctgatgg actatgaaaa tggttcctgg tggcaggagc
     8281 tggatgcgga caataaggtc atcaccaaag tctgggacgg caaacaggat atttatcacc
     8341 tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc cggcatggct ccagcggttg
     8401 cagcgggtct gctggatatt aatgcgaaat aactgatctg aaaatagggt gcgggttttc
     8461 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccggtgcag tttttgggtt
     8521 tgacatcagc ctcacgggag gctgtatgtc gttaattaag gtgccttgta tgcaaatcac
     8581 caatatgcat tgcagtggac agaccgtttc acttgccgcg ggggattatc acgcgaccat
     8641 tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag ggttgtcatt tagtcatccc
     8701 gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa gtgcttattc cgtggccgaa
     8761 ccggatcgcc aacggctgtt accgttatca ggggcaggaa tatcagttac ccattaatga
     8821 acatagttca aaagccgcta ttcacgggct gcttgcctgg cgagactggc aaattagtga
     8881 attaaccacc accagcgtta ccctgacagc ttttctgccg cccagttacg gctatccgtt
     8941 tatgctggct tcacaagttg tttattcgct caacgcacac acgggtcttt ccgttgagat
     9001 cgcaagccag aatatcggca ctgtcgcagc gccttatggc gtgggtattc atccttattt
     9061 aacctgcaac ttaacatctg ttgatgaata tctgttccag cttccggcaa accaggttta
     9121 tgccgtcgat gaacacgtaa atccgacaac gcttcaccac gttgatgagc tggatttaaa
     9181 tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac cacaccttta aaaccgctaa
     9241 tgatttgtgg gaaatgacca ttacccatcc acagcaggcg ctttcggtaa gcctttgttc
     9301 cgatcaaccc tgggtgcagg tttatagcgg tgaaaaatta cagcgtcagg gactggcagt
     9361 tgaacctatg agttgcccgc ctaacgcctt taattcaggt atcgacttat tattactgga
     9421 accaggaaaa ccgcaccgcc tgttttttaa tatttacgga cagagaaaat aacttgtatt
     9481 gattagcagt caaaatcatt gaagaacatt tcactcgcta aaggcaacat tatgtatctt
     9541 caatttatta agttaaaaac agctcagctt ctgtaaagtt tttatatatc agttttatga
     9601 gaatcatttt acataagcct gtaataacag gaaggcaaca atctcgttct caactctatg
     9661 gagattagtt atggatacgc cacgtccaca gttattagat tttcaatttc atcagaataa
     9721 cgacagtttt accctacatt ttcaacaacg tcttatttta acccataaca aagataatcc
     9781 ttgtttatgg attggctcag gtatagcgga tatcgatatg ttccgcggta atttcagcat
     9841 taaagataaa ctacaggaga aaattgcgct taccgacgcc atcgtcagcc agtcaccgga
     9901 tggttggtta attcatttca gccgtggttc tgacattagc gccacgctga atatctctgc
     9961 cgacgatcag ggccgtttat tgctggaact acaaaacgac aaccttaacc acaaccgtat
    10021 ctggctgcgc cttgccgctc aaccagagga ccatatctac ggctgcggcg aacagttttc
    10081 ctacttcgat ctgcgcggca aaccgttccc gctatggacc agtgaacaag gcgttggtcg
    10141 caacaaacaa acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg gcggcgacta
    10201 ttactggact ttcttcccac agcctacgtt tgtcagcacg cagaagtatt actgccatgt
    10261 tgataacagt tgctacatga acttcgactt tagcgccccg gaataccatg aactggcgct
    10321 gtgggaagac aaagctacgc tacgttttga atgtgctgac acatacatct ccctgctgga
    10381 aaaattaact gccctgctgg gacgccagcc agaactgccc gactggattt atgacggggt
    10441 aacgctcggc attcagggcg ggacggaagt gtgccagaag aaactggaca ccatgcgtaa
    10501 cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg tctgggatcc gtataacctc
    10561 ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc cgcaactgga
    10621 ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc ctggcctata tcaacccgta
    10681 tgttgccagc gataaagatc tctgcgaaga agcggcacaa cacggctatc tagcaaaaga
    10741 tgcctctggc ggtgactatc tggtggagtt tggcgagttt tacggcggcg ttgtcgatct
    10801 cactaatcca gaagcctacg cctggttcaa ggaagtgatc aaaaagaaca tgattgaact
    10861 cggctgcggc ggctggatgg ctgacttcgg cgagtatctg cccaccgaca cgtacctgca
    10921 taacggcgtc agtgccgaaa ttatgcataa cgcctggcct gcgctgtggg cgaagtgtaa
    10981 ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc ctcttcttta tgcgcgccgg
    11041 ttctaccggt agccagaaat actccaccat gatgtgggcg ggcgaccaga acgtggactg
    11101 gagtctcgac gatggcctgg cgtccgtagt cccggcggcg ctgtcgctgg caatgaccgg
    11161 acatggtctg catcacagcg acattggcgg ttacaccacc ctgtttgaga tgaagcgcag
    11221 caaagagctg ctgctgcgct ggtgcgattt cagcgccttc acgccgatga tgcgcaccca
    11281 cgaaggtaac cgtcctggcg acaactggca gtttgacggc gatgcggaaa ctatcgccca
    11341 ttttgcccgt atgactaccg ttttcaccac cctgaaacct tatctgaaag aggccgtcgc
    11401 gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg ttcctgcatt acgaagacga
    11461 tgcacatacc tattccctga aatatcagta cctgttaggc cgcgacattc tggtcgctcc
    11521 ggtacacgaa gaaggtcgca gcgactggac gctctatctg ccggaggata actgggtaca
    11581 tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc gttaatgcgc ccatcggcaa
    11641 gccgccggtc ttttatcgcg ccgatagcga atgggcggca ctgttcgcgt cgttaaacag
    11701 catctaatca catccgcccg tggggaaact catgggcaaa gggagaacat cgatgagtca
    11761 catcacaacg gaagatccag caactttacg cctgcccttt aaagagaaac tctcttacgg
    11821 tattggcgac ctggcctcta acatcctgct ggatatcggt acgctttatc ttttgaagtt
    11881 ttataccgac gttctggggc tgccaggcac ctatggcggc attatctttt tgatttcaaa
    11941 attctttact gcgtttaccg atatgggtac cggcattatg ttggattccc gacgcaagat
    12001 cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg tcattcccgg tcaccttact
    12061 ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc ggtaaaacgg tgatggccac
    12121 tattctgttt atgctttacg gactgttttt cagcatgatg aactgctcct acggcgcaat
    12181 ggtcccggca atcaccaaaa accccaacga acgcgcctca ctggcggcat ggcgtcaggg
    12241 cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc gtgccagtta tgaatcttat
    12301 cgaaggtaat cagaaacttg gctatatctt cgccgccacg ctgttttcac tgttcggcct
    12361 gctgtttatg tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa cccaaccaac
    12421 caatccggcg caaaagcctg gcctgttgca atctttccgc gcaattgccg gtaaccgccc
    12481 actgttcatt ctgtgtattg ccaacctctg caccttaggg gcgtttaacg tcaagctcgc
    12541 cattcaggtc tattacaccc agtacgtact taacgatccc atcctgttgt cgtatatggg
    12601 atttttcagc atgggctgta ttttcatcgg cgtgttcctg atgcctggcg cagtcagacg
    12661 ttttggtaag aagaaggtct atatcggcgg cctgctgatt tgggtgctgg gcgatctgct
    12721 caactatttc ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc tggcgttctt
    12781 cggctcagcg cctgaactgg gcgctggttt ccgacaccgt cgagtacggc gagtggcgca
    12841 ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac cttctttcgc aaagtgtctc
    12901 aggcgctggc tggtttcttc cccggctgga tgctgacgca aattggctat gtgccgaacg
    12961 tcgcacaggc tgaccacacc attgaagggt tacgccagtt gatcttcatc tacccaagcg
    13021 cactggcggt agtcaccatt gtggcgatgg gttgcttcta cagcctgaac gagaagatgt
    13081 atgtccgcat tgtggaagag atagaagccc gtaaacgcac ggcgtaataa taataaacga
    13141 cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg acattaaaac
    13201 tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat ttaatgctct
    13261 gcatcggtac tctgtatctc ctcaaacttt ataccgatga attagggatg cctgcttact
    13321 atggcgggat tatctttctg gtcgcgaagt tttttaccgc gtttaccgat atgctcaccg
    13381 gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt ccctttattt
    13441 tatatgcagc agttccggcg gcgttaattg caacgctcca gtttatcgcc accacctttt
    13501 gtttgccggt taaaacgacg attgccacca cgctgtttat gatgtttgga ctttcataca
    13561 gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc
    13621 gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg atttgtaccg
    13681 tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg
    13741 cacttatgtt ctccattggc ggctttattt ttatgatgct gtgctacaga ggcgtcaaag
    13801 agcattatgt ggacacagcg ccaaccggac ataaagccag tattctcaaa tctttttgcg
    13861 cgatatttcg taatccacca ttgctggttt tatgcattgc taacctgtgt actctggcgg
    13921 catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg aatgatatta
    13981 atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc gtattactgg
    14041 tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc atggtgctat
    14101 gggcggtggg tgatatactg aattatttct ggggaagtaa ctctttcact ttcgtcatgt
    14161 tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg gcactggtac
    14221 cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt tctgtttata
    14281 ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg ccaggcatta
    14341 tgctgacgca aattggttat gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt
    14401 tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca ttgacgatgg
    14461 gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa atcaaccaac
    14521 gcaaaaataa agagatggcg acagaaaaaa aaacggcttc cgtaacgtta taaatgtcaa
    14581 tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa tggacttatt
    14641 atgaaaaaga ttaatgcaat aattttatta tcttctctga cctctgcctc ggtatttgct
    14701 ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg cgaggttatg
    14761 ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa tacctacacc
    14821 tttcagcgag aagatgaact aaaacatggc tataatgaaa ttgaaggttg gtatccttta
    14881 ttcaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga taagagtatc
    14941 agttcgggtg gtgcagtgta tctggac
//
LOCUS       DAJVQR010000092        14479 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVQR010000092 DAJVQR010000000
VERSION     DAJVQR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(113..379)
                     /locus_tag="OQM52_003123"
     CDS             complement(113..379)
                     /locus_tag="OQM52_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1564879.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OQM52_003124"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OQM52_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1564880.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OQM52_003125"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OQM52_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OQM52_003126"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OQM52_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1564881.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OQM52_003127"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OQM52_003127"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1564882.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OQM52_003128"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OQM52_003128"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1564883.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OQM52_003129"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OQM52_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1564884.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OQM52_003130"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OQM52_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OQM52_003131"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OQM52_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="OQM52_003132"
     CDS             complement(12641..14095)
                     /locus_tag="OQM52_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCS1564885.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="OQM52_003133"
     CDS             complement(14092..>14479)
                     /locus_tag="OQM52_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1564886.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3860 a         3651 c         3418 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DAJVQR010000093        14422 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVQR010000093 DAJVQR010000000
VERSION     DAJVQR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..133)
                     /locus_tag="OQM52_003134"
     CDS             complement(<1..133)
                     /locus_tag="OQM52_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1564887.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            316..459
                     /locus_tag="OQM52_003135"
     CDS             316..459
                     /locus_tag="OQM52_003135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564888.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(678..2216)
                     /gene="aldB"
                     /locus_tag="OQM52_003136"
     CDS             complement(678..2216)
                     /gene="aldB"
                     /locus_tag="OQM52_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCS1564889.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2381..3532)
                     /gene="yiaY"
                     /locus_tag="OQM52_003137"
     CDS             complement(2381..3532)
                     /gene="yiaY"
                     /locus_tag="OQM52_003137"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1564890.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3722..5566)
                     /gene="selB"
                     /locus_tag="OQM52_003138"
     CDS             complement(3722..5566)
                     /gene="selB"
                     /locus_tag="OQM52_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1564891.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5563..6954)
                     /gene="selA"
                     /locus_tag="OQM52_003139"
     CDS             complement(5563..6954)
                     /gene="selA"
                     /locus_tag="OQM52_003139"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1564892.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7052..7660)
                     /locus_tag="OQM52_003140"
     CDS             complement(7052..7660)
                     /locus_tag="OQM52_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1564893.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7731..8816)
                     /locus_tag="OQM52_003141"
     CDS             complement(7731..8816)
                     /locus_tag="OQM52_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1564894.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8819..9181)
                     /locus_tag="OQM52_003142"
     CDS             complement(8819..9181)
                     /locus_tag="OQM52_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1564895.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9718..11631
                     /gene="mtlA"
                     /locus_tag="OQM52_003143"
     CDS             9718..11631
                     /gene="mtlA"
                     /locus_tag="OQM52_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1564896.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11726..12874
                     /gene="mtlD"
                     /locus_tag="OQM52_003144"
     CDS             11726..12874
                     /gene="mtlD"
                     /locus_tag="OQM52_003144"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1564897.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12874..13461
                     /gene="mtlR"
                     /locus_tag="OQM52_003145"
     CDS             12874..13461
                     /gene="mtlR"
                     /locus_tag="OQM52_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1564898.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13473..13682)
                     /locus_tag="OQM52_003146"
     CDS             complement(13473..13682)
                     /locus_tag="OQM52_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564899.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13966..14328
                     /locus_tag="OQM52_003147"
     CDS             13966..14328
                     /locus_tag="OQM52_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1564900.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3523 a         3842 c         3625 g         3432 t
ORIGIN      
        1 gaatggcgtc ggcatgagga tgattacgtt ttttagcaat caggtaggga atatcatgta
       61 aaataataat cccatagaat atcaccagaa atacaaaaat aagcactccc agtgcaaaat
      121 agtccaggaa catatttccc tcaaagaata taaaaaagaa caattaacgc atattatgaa
      181 atgccatgat gcagtgcaat ttccttattt atataacgac atattgtttt ttatatactt
      241 tctgaaatgt gctgtctggt ttttcaacag gacgaggttt atcatattga aatagtggaa
      301 ctattggcca aactaatgaa taactccagt aaaacatcca cagtacagat taagcgtatt
      361 aaaccttcaa ttatctaccg tttattgctg attggcctcg gatcaccaat ggtgatttac
      421 ggcctggttc gcccgctcac catcgaaacg cgagattaaa ctcctgacaa aacacgcccc
      481 agaaacgcac ttctgttggc gtagtctggg ttattgcgca cgtaggttta aaacgtaaat
      541 tgtaacaacg tgcgctttgt ttatgccgga tgcggcgtgt acgccttatc cggcctacgg
      601 tcaggttccc gtaggcatga taagacgcgt aagcgtcgca tcaggcaatg aatacccaat
      661 gcgaccagct tcttatatca gaacagcccc aacggtttat ccgagtagct caccagcagg
      721 cacttggttt gctggtaatg ctccagcatc atcttgtggg tttcgcgacc gatacctgat
      781 tgtttgtagc caccaaacgc agcatgtgcc gggtaagcgt gataacagtt ggtccacacg
      841 cgcccagcct gtatgccgcg ccccatctta taggccagat taccgttgcg gctccagacg
      901 cccgcgccca ggccatattg cgtatcgttc gccagctcca gcgcttcttc catcgttttg
      961 aaggtggtca ccgccagcac cgggccaaaa atctcctcct ggaacacccg catattgttc
     1021 tgaccaaaca gaatcgtcgg ttcgaggtag tagccgtctt tcagttcacc ttccagcagc
     1081 ttgcgccgcc cgcctgtgag cacgtcagcg ccctcttttt taccgatatc aatgtagttg
     1141 aggatggttt ccagttgccc gtgagaaacc tgcgcgccca tttgcgtcac gctgtcgagc
     1201 gggttaccgc tgcgaatgct ttcgacacgg cggatggcgc gttccataaa gcgttcgtag
     1261 attgactcct gcactaaggc acgactcgga caggtgcaaa cttcgccctg gttaaaggca
     1321 aacagtgcaa agccttccag cgctttatcg aaaaaggcat cttcttcatc catcacatca
     1381 gcaaagaaga tatttggcga cttaccgccc aactccagcg tcaccggaat aatgttttgc
     1441 gttgcgtatt gcataatttg ttggcccact tccgttgagc cggtaaacgc cactttggcg
     1501 atgcgtttcg aggtcgccag atattcgcca atttccccac ctgcgccatt gaccacgttc
     1561 accacgcccg gcggcagtaa atcaccgaca atttccatta gcagcagtac agaaagcggg
     1621 gtaagacgtg cgggtttcag caccacacag ttgcccgccg ccagcgcggg agccattttc
     1681 cagctcgcca tcagcagcgg gaagttccac gggataatct gccccaccac gcctaacggt
     1741 tcatggaaat gataggccac ggtttcgcta tcaacttcac tgatcccacc ttcctgcgcc
     1801 cgaatacacg aggcgaaata gcggaaatgg tcaatcgcca gcggtacatc cgcagcactg
     1861 gtttcgcgaa tgggtttgcc gttatcccag gtttcagctg tcgctaacag ctcgaggttt
     1921 tgttccattc gatcggcaat cttaaacaga atcgccgcgc gatcctgcac cgaggtgtgc
     1981 gcccatttat ctttcacttt gtgcgcagca tccagcgcca gatcgatgtc tcgtttgccc
     2041 gaagacgcca cttcgcacag cagctgcccg gtcaccggcg tcagattctg gtaatactca
     2101 ccatcggcag gggctaccca ttcaccgcca ataaagttgt cgtagcgggc ttttaacttg
     2161 atggggaaac catactcgcc gggcttaatc tgtgctgaag ggggattatt ggtcatgatc
     2221 gtctccttgc ggtgtgaggt ataacaaggg tagacgtgac tggcgaaatc ttcgccagtc
     2281 ggtaacagct ttacgacagc tatcacgaat ttacgggcaa ggattacaga gatagttgaa
     2341 aaagcggctg acaatttgcc agccgttgtt ggaatgatga ttacattgcg gcgcgataaa
     2401 tcaccacaat ttcttcgtga gttgcctgga tcgggttagt aaagccacag gcatctttca
     2461 gggcattagt cgccagaacc gcgaaatctt cttctttcac gttcaggtcg cgtaggcctg
     2521 ccgggatatc cactttcttc gccagttcac ggatggcgtt aatgcaggct tcagcacctt
     2581 ccgcgtcgtt tttacctgtc acgttcacgc ccattgcagc ggcacagtca cgcagacgtg
     2641 cagcggcgac tttgctgttg aatacctgaa catgcggcag cagaacggcg ttacatacac
     2701 cgtgtggcag gttgtagaaa ccgcccagct ggtgcgccat cgcatgaaca taacccagag
     2761 aagcattatt gaacgccata ccggcaagga actgggcata agccattgct tcacgcgctt
     2821 tcgcattact gccatcttca acggctaacg gcaggttttc ggcaatcatg gtcacggctt
     2881 tcagtgcaca agcgtcagtg atcggcgtgg cggcaataga aacatacgct tcgatagcgt
     2941 gcgttaaggc atccataccc gttgcggcgg tcagtgactt cggcatacca atcatcagag
     3001 aggagtcatt gacagaaagc agcggagtca cgtgcttatc aacaatcgcc attttgttat
     3061 gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc agaggccgta cccgccgtgg
     3121 tattgatggc gatcatcggc agctgcggtt ttgcagagcg gtcaacgcct tcgtaatcgc
     3181 gaatatcgcc gccattggct gccaccagcg caataccttt tgcgcagtcg tgtggagaac
     3241 caccgcctaa ggagatcacg ctatcgcaat tattttcttt aagtaatttc aaacctgcgg
     3301 cgacgttttc cgtggtgggg ttaggttggg tgccatcata aataacgcta aaaatattgc
     3361 gttcttccag tgctttttgc acatcacccg ccatacctaa tttcgttaac atattgtcag
     3421 tgacaattaa ggtacgggta aatccataat ctgccatcat attcattgca tcagtcaatg
     3481 aatcagcgcc gatgacattc acagaaggaa taaagaaagt tgaagatgcc ataacacact
     3541 cctgaaagtg taaaaaaggg tgggcagctt actgaggatt ttcattcgaa aacatgatca
     3601 ttattattaa ttatcaaaat taatctaata aaaagcacat tgtattcatt aaacaatgtg
     3661 ctttatttaa aatcataaga aatttgtatt aatttatttt aaaaattaat taatcatttc
     3721 cttatttttc cggaaataat aatgcgtcgc gcaataaatg atcatttcca cgacgacgcg
     3781 taaagccaat gcggtcaaaa tattccagaa tctgaattgc cagctttcgg cctacgccta
     3841 agcgatcgcg gaaatccgcc gcgcaggttg aaccacactc ctgatcgaga tcgcggatca
     3901 tattggcaaa ttcgacaatc cgatcgttac ggtaataacg atctttgacg atcgcggtaa
     3961 ttattccttg ctgcgccgcc tggcgtagcg tcaggcgcat tgcctgctcg tcggtacccg
     4021 tctcttttgc cagatcacgc acccaccacg gctcgtcacc aaacagcggc tctgcttttt
     4081 gccagatggc ctgctgctct tcgctgaagc ccgctttgtg atccggcaga tgcagccaac
     4141 cgtgatgact gaggatgtcg ccgctttcgc gcatcttttc aatcagcaac agtactagcg
     4201 cttcatcttc cattggcaac gccatacgtc gcagacgttc gcgcccaggc ccaggttcat
     4261 cgcgatgttg ctcatgataa gtcgctaatg tgtcgagaat tttccgttgc cagcgagcgg
     4321 caaccggcgc attcaacaag ctataaccag cctgaatata gccaggctgt tgcagcagtt
     4381 cgcgcatccc ttcgccgttg agctggcgcg cccaagcgaa atcaggaaga ttaaccgcgc
     4441 cgcgttgcag gtgaacggct aacgcttcgc tatcattctg tgccctggcg agggaagcca
     4501 gccattgcag atactctggt ttacgtttac cgcgacgcgg cgggttaagc atcacgacgc
     4561 gcgctccggc cagcgtgttg cgggcagaga tatcgcgcaa taccaggcgg tcgttatctg
     4621 ccagccataa cggggtgtcg aagaccagtt cagcaaggtt atcttccagc agtgaaacgc
     4681 gtcccgtgac gtggctggcg gcgtggtgaa tatgcagcgg ctgccactgg gtcagcggtg
     4741 catgggtttg aagctcgaca atcacccgtg tgaacggctc tggcggcaca tcggcaagca
     4801 gccagtcgcc acggttaatc tgctcttttt ccgcatcacc cgcgatgtca agcgcgatac
     4861 gctgcccggc attggcggtt tctgttggct ggttttgcgc atgcagcgca cgtacacgca
     4921 tcggtttatt tacaccagtc agccagagtg aatcgcctac cttcacttcc ccgcttaacg
     4981 ccgtaccggt gacgaccagc ccggcacctt ttacggtaaa tgcgcggtca atcgcgaggc
     5041 ggaaactatg ttggctggcg tgctcgcgtt ccggcaactg aagcagatgc tcgcgcaggg
     5101 catcaattcc ccgaccttcg gttgctgcgg tgataaacag ttttgcctca gcaaaaccgt
     5161 attcccgcag aacctccttt acctggcgtt caacctcatc aacacgcgct tcgtccacgc
     5221 gatcggcttt ggtcagcgcc actgtcagca tcgggttacc ggtcagctgc aaaatcgcca
     5281 gatgctcacg ggtctgtgcc atcacgccgt catcgcacgc caccaccaac agcgcgtgat
     5341 cgataccacc aacgcccgcc agcatgttgg aaagaaactt ttcatgaccg ggaacgtcga
     5401 taaaaccagg cacgcgacca tccggctgcg gccagtaggc atagccgaga tcgatggtca
     5461 tgccgcgctt tttttcttcc ggcagacggt cagcatttac gccagtaatc gcctgcaata
     5521 aggttgtttt gccgtggtca acgtgtccgg cagtcgcaat aatcatttca acaacatctc
     5581 caaaaaccgt tgctcatctt caaggcagcg taaatccagc cacaatcgtc cgtcataaat
     5641 acgaccaatc accggcactg gcaattcacg ccagcgggcg gctaatgact caaggtggct
     5701 accgcgtcca tcatggggtg taaacgttaa tgccgcgctc ggcaggcgat caaccggcag
     5761 cgaaccactg ccaatctgcg aaagacatgg cataacctgt accgcaaact ccgcgccgta
     5821 atgtgcgaca aggggagcct gtaaacgttg tgcctggatt tgaatgacct ctgcgctgcg
     5881 ggtaagcagg cgcagggtcg gtaatttttc actcagagct tcagggtgta aataaagacg
     5941 caacgtggct tccagcgccg cgagggtcat tttatccgcg cgtaatgcac gcttcagcgg
     6001 gtggctttgc aggcgggcga tcatctcttt tttaccaacg ataatcccgg cctgcggccc
     6061 gcctaacaac ttgtcgccgg agaagctcac cagactgacg cccgccgcaa tcaactcctg
     6121 cggcattggc tctttcggca aaccgtactg gctaagatcg accagcgagc cactgcctaa
     6181 atcagtcacc actggaacat ccagctcttt gccgagcgcc accagttcag cttcgtctat
     6241 cgctttggtg aacccctgaa tgctgtagtt actggtatgt actttcatca acagtgcggt
     6301 attttcattc accgcctgac gataatcatt cgcgtgcgtg cggttggtgg tccctacttc
     6361 gtgtagggtg cagcctgcct gacgcataac atcgggaata cgaaacgcgc cgccaatctc
     6421 caccagttcg ccgcgagata ccaccacctc tttcccgctg gcagtggccg ccaacatcaa
     6481 taacaccgcc gccgcattgt tattgacgat acaggcatct tccgcccccg taatacggca
     6541 cagcagctgc gccagcgccc gatcgcgatg tccgcgtccg gcgtcgtcca gatcgtactc
     6601 gagggtcact ggcgaacgca tagcctgcgc aacggcttcc accgcggctt ccgcctgtaa
     6661 agctcgccca aggttggtat gcagcacggt tcccgtcagg ttgatcaccg gacgcagcgc
     6721 gctctgcgcc tctttcgtca accgggcatc gacttcttgc gcccagtttt cacaccacgc
     6781 aggcagcgtc tggctgccac gaatcacttc tcgcgcttcg tcgagcatct gacgcaacaa
     6841 ttccaccacg cgggtgtgac cataagtatc acgcaaagaa aggaaggagc tatcgcgcaa
     6901 taagcgatca atagccggaa gttgactata gagggaacgc gtttcggttg tcataggaaa
     6961 cctggctgat caaggccctc tcacacggag aagggcgttt aacataacca cggattgtaa
     7021 cgtgagatgg gtcaggagga catatcgcgc atcaagcctt tggcggttcg gtgcgagcaa
     7081 aactttcgcg gctaaacagg ttttcgacca gtttgactaa gtgtgggcgg tcgacacacc
     7141 aacctggcgc aacgcggcgg aaattgagat atccgacagc acaggcaatg gcgatagtcg
     7201 ccagattgac cgtatcggtt ttgagtgtgc catcgaccag atatccttcc agcacatcca
     7261 gactgcggtt gattttctcc cgctggcgta acaattcatc ttcagactgc tgcgccgccg
     7321 gacgcgcctg ttcacgcacc gataccagcc cggcatccat aatgccatcc gccagcgcct
     7381 caattttgcg cacccgcaac gactccagcg gatcgcgcgg caacatcgcc ggagcgacat
     7441 tcattaactc aatatattca gcgatgatcg gcgaatcaaa ccagcattcg ccctcttcgg
     7501 tcaccagcac cggcactttc cctaacgggt taaattgcgc cacgccgtta tccgcgttat
     7561 agggcagttc attaataaat tcgaaagtta tgcccttttc taacaacaga ataaaaagtt
     7621 tgcgtacaaa cgggctggtg tagctaccga cgagtttcat gccgagtcct ttgtgcgagg
     7681 aaaaatatca gtatggctca ggctcacgca aaacacaggt gatataagtc tcaatgatcc
     7741 aaataaagat aatgcatcca actggtcatt cttagcagca ctttccgcat caccgaaaca
     7801 tggctgaaat ggtcggagta aaccgccacc tgggcgtaga tgccgtcggg taaggcatcg
     7861 atatcatcgt tagggtccag ttcaatggtt cccagcacac cgtccgtgcc gggaacgacc
     7921 gttaatgatt gcaatacccc ctgcgcctga taagaaccgc ctggcacgac aggtaaaata
     7981 ctagtcaatt tgccgtggaa cacctgccca ggtagcgcgt taaacaccac ttccgcatca
     8041 tcgccaggtt tcagacgtaa cagcgagttt tgccgaaatt gggcgacaat ttgccgtttt
     8101 tgctcgggga tgaagaccat caccggacgc agcggcaagg cagctgcgta tgtacctggg
     8161 cggatcagta cctgagtgac gtagccattg cttggtgcgc gaatgacagt ctgctcaaga
     8221 ttatattttg cttcagtaag ttgcgctctt aagctcacaa tctgcgattg ctcgccgttg
     8281 accatactgt cgagctgact ctggatctgc gcctgctccg ccaccgagcc tttcactaac
     8341 gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt cacgttccga gaacggattc
     8401 accgccgcct ggctgccttt caggtaacgt tgataatttt taaacagacg gtcgcgctcc
     8461 gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct gcgcacgcag cgtctttata
     8521 ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa ctcgcgcctg gtaacgaacc
     8581 gggtcgagct taaaaagcac ctcgcccttt tgaataagct gattattctt gtcagtgact
     8641 tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg ctatcactgc cttttgcgcg
     8701 gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca aaccactcac cagaaacacg
     8761 cctcccagcg ccgccgtcgc cagcgccacg taagttaaaa caatcaatag atccataatt
     8821 agatctccgg gaacgtggaa tagtcagtct ttttctcggc ggcgagttga tgctccagcc
     8881 gggaaattcg gtcagaaagt gcggcgattt ccggttcagt cgctttctcc tgcgacgcaa
     8941 catgtgactg cataccccag ccacgctccg gttgatagag cgtcgcccag atccacagaa
     9001 acggccagat aacatgcaga gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg
     9061 catgggggtg attgcgcttt ttggcaatca gataagggat gtcatgtatc gcgatgattc
     9121 cataaaaaat caccagaaag acgaagatca gcactcccaa cgcgaaatag tttagaaaca
     9181 tatctgcctc ggattcacgt ttatcagtgt tgttttttgg ctggcagcca gaagggagtc
     9241 aggctgatat tttgacaata atccgggttc gcgattctcg ccataacacc aaagaataat
     9301 ttttagaggt gatgagttgc ttagttacat aacgattgta tgacgaaggc ataacatgct
     9361 gtagatcaca tcaggtgaac gccgtaagaa aatatcttgt gattcagatc acaaagattc
     9421 aacaaaccat caaaacaaaa atgtgacatt actcacattt aaatgccatt tttagcgaaa
     9481 atcaccgcct tgtttctttt ttatacaggc gttttgtgat gagcgtcacg tcaattacct
     9541 ctctaccccc tatatttatg tgactgatat cacacaaaag cccgtcgact ggacagttaa
     9601 ccgattcagt gccagatttc gcagtatcta caaggtccgg ctacctctgc cgccacatta
     9661 acaaaaaacc tcgggcttcc agcctgcgcg acagcaaaca taagaagggg tgtttttatg
     9721 tcatccgata ttaagatcaa agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg
     9781 ccaaatatcg gcgcgtttat cgcgtggggt atcatcaccg cgttatttat tccaacaggg
     9841 tggttaccga acgagacgct ggcgaagctg gtcgggccga tgatcactta tctcctaccg
     9901 ctgctgatcg gttataccgg tggtaagctg gtaggcggca aacgtggtgg cgtagtcggt
     9961 gccattacca ccatgggcgt tatcgtcggc gcagacatgc cgatgttcct cggttctatg
    10021 attgcaggtc cgctgggcgg ctggtgcatt aagcacttcg accgctgggt agacggtaag
    10081 atcaaatccg gttttgagat gctggtgaat aacttctccg caggcatcat cgggatgatc
    10141 ctcgctattc tggcattcct cggcattggc ccgattgttg aagccctgtc caaaatgctg
    10201 gctgcgggcg ttaacttcat ggttgtccat gacatgctgc cgctggcgtc tatctttgtt
    10261 gaaccggcga aaatcctgtt cctcaacaac gccattaacc acggtatctt ctcgccgctg
    10321 ggtattcagc agtcccatga actgggtaaa tcaatcttct tcctgattga agctaaccca
    10381 ggtccaggta tgggcgtgct gctggcgtac atgttctttg gtcgtggtag cgctaaacag
    10441 tctgcgggcg gtgcggcaat catccacttc ctgggtggta tccacgaaat ctacttcccg
    10501 tatgtgctga tgaatccgcg tctgatcctc gcagtcatcc tcggcggtat gactggcgtg
    10561 ttcacgctga ctatcctggg cggtggtctg gtttctccgg catctccggg ttctatcctt
    10621 gctgtactgg cgatgacacc aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg
    10681 gcgatggctg tctccttcgt tgtctctgct attttgctga aaaccagcaa agtgaaagaa
    10741 gaagatgata ttgaagcagc aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc
    10801 acatctccgc tatctgcggg cgatgtgact aacgacctga gccacgtacg taaaatcatc
    10861 gttgcctgtg acgccggtat gggttcaagt gcgatgggcg caggcgtgct gcgtaagaaa
    10921 attcaggatg caggtctgtc gcagatttcg gtcactaaca gcgcgatcaa caacctgccg
    10981 ccagatgtgg acctcgtcat cactcaccgt gacctgaccg aacgcgctat gcgccaggtt
    11041 ccgcaggcac agcatatttc gctgaccaac ttcctcgaca gcggcctgta caccagccta
    11101 accgaacgtc tcgttgctgc ccagcgccat actgaaaacg aagtgaaagt gaaagacagt
    11161 ctgaaagaca gctttgacga ttccagtgct aacctgttca aactgggcgc ggagaacatc
    11221 ttcctcggtc gcaaagcagc aaccaaagaa gaagcgattc gttttgctgg cgagcagctg
    11281 gtgaaaggcg gttacgttga gccggaatac gttcaggcga tgctggatcg tgaaaaactg
    11341 accccgactt atctgggtga gtctatcgcg gtgccacacg gtacggttga agcgaaagat
    11401 cgcgtactga aaacgggcgt ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa
    11461 gaagaagatg acattgcccg tctggtgatt ggtattgcag cccgtaacaa cgagcacatt
    11521 caggttatca ccagcctgac caatgcactg gatgatgagt ccgtcatcga gcgtctggca
    11581 cacaccacca gcgtggatga agtgctggaa ctgctggcag gtcgtaagta atccaatcct
    11641 accctctcct cacggagagg gtttgggtga gggaaaagcc tcaccccagc cctctcgggt
    11701 aaaaacattg atgaaggtta atactatgaa agcattacat tttggcgcag gtaatatcgg
    11761 tcgtggcttt atcggtaaac tgctggcaga cgcgggtatc caactgacgt ttgccgatgt
    11821 caatcaggtg gtacttgatg ccctgaatgc ccgtcatagc tatcaggttc atgtggtcgg
    11881 tgaaaccgag caggtagata ccgtttccgg cgtcaatgct gtcagcagca ttggtgatga
    11941 tgtcgttgat ctgattgctc aggtcgattt agtcactacc gccgttggcc cggttgtgct
    12001 ggaacgtatt gctccggcta tcgccaaagg gctggtgaaa cgcaaagaac aaggtaatga
    12061 atccccgctg aacatcatcg cctgtgaaaa catggtacgc ggcaccacgc agctgaaagg
    12121 tcatgtgatg aacgccctgc cggaagacgc caaagcgtgg gtagaagagc acgttggctt
    12181 tgtcgattcc gccgttgacc gcatcgtacc gccttcggct tcggcaacta acgatccgct
    12241 ggaagtgacg gtagaaactt tcagcgaatg gattgtcgat aaaacgcagt tcaaaggcgc
    12301 actgccgaac atcccaggca tggagttaac cgacaacctg atggcatttg tcgaacgtaa
    12361 actcttcacc ctgaacacgg gtcatgctat aaccgcgtac ctcggaaaac tggccggtca
    12421 tcagaccatt cgtgacgcta ttctcgacga gaaaatccgc gcggtggtaa aaggtgcgat
    12481 ggaagaaagt ggtgcggtac tgatcaagcg ctacggcttt gacgcagaca aacatgcggc
    12541 gtacatccag aaaatcctcg gtcgttttga gaacccgtat ctgaaagatg atgtagagcg
    12601 cgtaggccgt cagccgctgc gtaaactgag tgctggcgac cgtctgatca agccactgct
    12661 cggtacgctg gaatacagcc ttccgcacaa gaatctgatt caggggattg ctggtgcaat
    12721 gcacttccgc agtgaagatg atccgcaggc tcaggaactg gcagcactga tcgctgacaa
    12781 aggtccgcag gcggcgctgg cacagatttc cggtcttgat gccaacagcg aggttgtatc
    12841 cgaggcggta accgcttata aagcaatgca ataatggtgg accaggcgca ggacaccctg
    12901 cgcccgaata acagattgtc agatatgcag gcaacaatgg aacaaaccca ggccgttgaa
    12961 aaccgtgtgc ttgagcgtct gaatgctggc aaaaccgtgc gaagctttct gatcaccgcc
    13021 gtcgagcttt tgaccgaggc ggtaaatctt ctggtgcttc aggtattccg caaagacgat
    13081 tacgcggtga agtatgctgt agaaccgtta ctcgacggcg acggtccgct gggcgatctt
    13141 tctgtgcgtt taaaactcat ttacgggttg ggcgtcatta accgccagga atacgaagat
    13201 gcggaactgc tgatggcatt gcgtgaagag ctaaatcacg acggcaacga gtacgccttt
    13261 accgacgacg aaatccttgg accctttggt gaactgcact gcgtggcggc gttaccaccg
    13321 ccgccacagt ttgaaccagc agactccagt ttgtatgcaa tgcaaattca gcgctatcaa
    13381 caggctgtgc gatcaacaat ggtcctttca ctgactgagc tgatttccaa aatcagctta
    13441 aaaaaagcct ttcaaaagta agcaacgtct gcttactgcc cctctacctg cttcggccga
    13501 taaagccgac gataatactc cagacgttgt agatataacg gcacgctttc atcgggtatt
    13561 ccggacggaa tcgcgttacg gggaggaagt tttttcagat actcccggaa cgcctggctt
    13621 gatgccatga aatctacggc tttatcgata agaagcggaa cgttttcacc taatttgccc
    13681 atgatgatat ctccgtatta ccccgcgccg ggaatgcgcg gccgccaatt ttagtttagg
    13741 ttcccggtaa agatgacatt aaggaaaacg tgctgaatcc tcaataatca tccgctgctg
    13801 tttattatct ttttctatag atttcttaat taatcaacga attgcgtagt ttttgagcat
    13861 gttaatgatt acgttgcaga actattacaa tgtcatcagt tgtgaacaaa gcacctggtc
    13921 gcgcatacta ggggctataa atttatcttt atcagaagcc atcacatgaa agaagtcgaa
    13981 aaaaacgaaa tcaagcgtct cagcgatcgc ctggacgcca tccgccacca gcaggccgat
    14041 ctgtcgctgg ttgaagccgc agacaaatat gccgagctgg aaaaagagaa agccacgctg
    14101 gaagcagaaa ttgctcgcct gcgtgaggtt catagccaaa aactgagtaa agaagcacag
    14161 aagctgatga agatgccgtt ccagcgcgca attaccaaaa aagagcaggc tgatatgggc
    14221 aagctgaaga aaagtgttcg cggactggtg gttgtgcacc caatgaccgc gctggggcgc
    14281 gaaatgggcc tggaagagat gactggattt tctaagacta cgttttaagc ccctcagacg
    14341 actggttcga tcgtatcaac ccgcatctgg cttgccttag ctggtcaatg cggggactct
    14401 cctgtctact cgcctaatta cc
//
LOCUS       DAJVQR010000094        14316 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVQR010000094 DAJVQR010000000
VERSION     DAJVQR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..3051
                     /locus_tag="OQM52_003148"
                     /pseudo
     CDS             1..3051
                     /locus_tag="OQM52_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="OQM52_003149"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="OQM52_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="OQM52_003150"
     CDS             6768..7175
                     /locus_tag="OQM52_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564901.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="OQM52_003151"
     CDS             7717..8805
                     /locus_tag="OQM52_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCS1564902.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="OQM52_003152"
     CDS             8816..11095
                     /locus_tag="OQM52_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564903.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="OQM52_003153"
     CDS             11088..12224
                     /locus_tag="OQM52_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1564904.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="OQM52_003154"
     CDS             12221..14224
                     /locus_tag="OQM52_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564905.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3755 c         3655 g         3360 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccatcagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcac
//
LOCUS       DAJVQR010000095        14251 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVQR010000095 DAJVQR010000000
VERSION     DAJVQR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..104)
                     /locus_tag="OQM52_003155"
     CDS             complement(<1..104)
                     /locus_tag="OQM52_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCS1564906.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OQM52_003156"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OQM52_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS1564907.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="OQM52_003157"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="OQM52_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1564908.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="OQM52_003158"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="OQM52_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS1564909.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2423..2839
                     /gene="csgF"
                     /locus_tag="OQM52_003159"
     CDS             2423..2839
                     /gene="csgF"
                     /locus_tag="OQM52_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS1564910.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2866..3572
                     /locus_tag="OQM52_003160"
                     /pseudo
     CDS             2866..3572
                     /locus_tag="OQM52_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3705..4196)
                     /locus_tag="OQM52_003161"
     CDS             complement(3705..4196)
                     /locus_tag="OQM52_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1564911.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="OQM52_003162"
     CDS             complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="OQM52_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1564912.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="OQM52_003163"
     CDS             complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="OQM52_003163"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1564913.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="OQM52_003164"
                     /pseudo
     CDS             complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="OQM52_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6841..6928
                     /locus_tag="OQM52_003165"
     tRNA            6841..6928
                     /locus_tag="OQM52_003165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6875..6877,aa:Ser,seq:gga)
     gene            complement(7078..7359)
                     /locus_tag="OQM52_003166"
                     /pseudo
     CDS             complement(7078..7359)
                     /locus_tag="OQM52_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7641..8645)
                     /locus_tag="OQM52_003167"
     CDS             complement(7641..8645)
                     /locus_tag="OQM52_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCS1564914.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8717..9277)
                     /locus_tag="OQM52_003168"
                     /pseudo
     CDS             complement(8717..9277)
                     /locus_tag="OQM52_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9362..9559)
                     /locus_tag="OQM52_003169"
     CDS             complement(9362..9559)
                     /locus_tag="OQM52_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564915.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9571..10062)
                     /locus_tag="OQM52_003170"
     CDS             complement(9571..10062)
                     /locus_tag="OQM52_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564916.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10059..10433)
                     /locus_tag="OQM52_003171"
     CDS             complement(10059..10433)
                     /locus_tag="OQM52_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1564917.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10480..10854)
                     /locus_tag="OQM52_003172"
     CDS             complement(10480..10854)
                     /locus_tag="OQM52_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCS1564918.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11017..11238)
                     /locus_tag="OQM52_003173"
     CDS             complement(11017..11238)
                     /locus_tag="OQM52_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1564919.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11301..11777)
                     /locus_tag="OQM52_003174"
     CDS             complement(11301..11777)
                     /locus_tag="OQM52_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCS1564920.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11793..12266)
                     /locus_tag="OQM52_003175"
     CDS             complement(11793..12266)
                     /locus_tag="OQM52_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS1564921.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12608..13429)
                     /locus_tag="OQM52_003176"
     CDS             complement(12608..13429)
                     /locus_tag="OQM52_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1564922.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13529..13702)
                     /locus_tag="OQM52_003177"
     CDS             complement(13529..13702)
                     /locus_tag="OQM52_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCS1564923.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13822..>14251)
                     /locus_tag="OQM52_003178"
     CDS             complement(13822..>14251)
                     /locus_tag="OQM52_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564924.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3642 a         3492 c         3411 g         3706 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atggctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gtggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagactcc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac ggacgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gcgttagata actttactca
     2641 ggccatccag tcacaaattt taggtgggct actgtcgaat attaataccg gtaaaccggg
     2701 ccgcatggtg accaacgatt atattgttga tattgctaac cgcgatggtc aattgcagtt
     2761 gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa
     2821 taactcaacc gatttttaag ccccagcttc ataaggaaaa taatcatgca gcgcttattt
     2881 cttttggttg ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa agaagccgcc
     2941 agaccgacat taatgcctcg tgctcagagc tacaaagatt tgacccatct gccagcgccg
     3001 acgggtaaga tctttgtttc ggtatacaac attcaggacg aaaccgggca atttaaaccc
     3061 tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg ccagggctta caaaacctgc
     3121 ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     3181 gaatcccgct gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt
     3241 atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca
     3301 cgcaatacca gctcgatcag attgccgtga acctgcgtgt cgtcaatgtg agtaccggtg
     3361 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     3421 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg
     3481 aacccgttat gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     3541 atggtatcga ccgtggtctg tgggatttgt aaaataaagc agaacggcag aatgacattc
     3601 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa atgccggaat
     3661 taataatccg gcatttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     3721 atttcctgct cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     3781 ttcattgcgt aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca
     3841 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     3901 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga
     3961 atttccagat gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     4021 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaagcaaag
     4081 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     4141 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     4201 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     4261 aattatctgt tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca
     4321 gctcgtccca cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag
     4381 gcgtggttgc atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact
     4441 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     4501 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgccagt agcatccctc
     4561 gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     4621 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     4681 tcatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     4741 cagccagttt cccctcacga atcagagtaa acagcggcac cagtaaagga tcttgcggtt
     4801 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     4861 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     4921 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctagcg gaaaatctac
     4981 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     5041 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcagctaccg cacgacagtt
     5101 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     5161 atgtttcgcg gctgcttcag caacggcttt aacatctatt tcatatttcg gatttccggg
     5221 atggcttatt atatgcacat tgccgctggc gatagtggag atcatcgctt gtgtattggt
     5281 cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     5341 gtcaaacatt ttaccgctgc agtcaatttc accatcaaca tttttaatgt tagcttcaat
     5401 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     5461 ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     5521 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     5581 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     5641 aaggatagca ggaatcctga tgctttatta gtagccacgt gcgcggtcga cttgcccgca
     5701 gaccctctcc cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc
     5761 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     5821 actttcaggc ggtaagggtt cacgattaaa aacatccagc attgcgcctt taactttccc
     5881 gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag
     5941 gagatacgcg ccatcaggta atttttcgag taattgttga ttaataatgc cgacggtttc
     6001 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     6061 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     6121 cagcggaaag cgctaggttt gcagactctg agcaacttta cagcccagta cgcctgcgcc
     6181 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     6241 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     6301 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     6361 gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     6421 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     6481 aacataatca gcagagtcat tatctccgct tttccatgcc ctgactcttg cctgaggaat
     6541 agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     6601 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     6661 acaaaggcca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat
     6721 taaaaccaca cattgattga atgtttgaat aaacgcgcga ttttttcaaa aagtttgttg
     6781 acctcaggtc atgatttccc taaattagcg cccgttccag caagacagga acgacaattt
     6841 ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag tgtgtatacg gcaacgtatc
     6901 gggggttcga atccccccct caccgccaga ttataagtgc atgttattgt ttaacatgcg
     6961 cttttttatt tttccttcca gacagtatgc catcgggtat gccatctgaa accggtttat
     7021 atgccccgcg atcatatctt atcggacaat ttccgatctt tgagcatact ggcaaagcta
     7081 tactgccagc atatcaggat agtgttttgc gataatatca ttcagtctgt caaccagttc
     7141 tggttttctg aacgtgatat gcgcggagcc cttcttaaag tatctgatgc tgaacatctc
     7201 atcttcatag ctctctttac cctgaacaga ctgaatgtga tcatccagac gaagggtgat
     7261 gtttccccgg ttgtcgggga ttggtttacc gctgaacagg tgcaacattc tttccagatc
     7321 ggcgagtcgg tcagcctgtt gtccggtgat aagatgaaat ctatggtcac ccccgttttt
     7381 gcaacactga tttttgatta atggttggct tgcttaaatc tatccggcgt ctctgtgggc
     7441 aaggatgccc gcgccccgat gagttccgca cctgataatc ctaaaaatcc cctcggcttc
     7501 agtgagccat ttcatctgtt aggtttttaa tcaggtcgat ttgccgttca tgtcatcaat
     7561 aatcagtctt cgcaaaacca gaaaggtaac ttgtatctta ttgtttaaaa taaactaatt
     7621 gagttatatc agcatctata tcaggcctga tattcttttt tctgagtagt gattgcccag
     7681 gcaattcgag ccagtttatt tgccagagcg caggtaacca cattcgaatg cttcttgttg
     7741 agttgctccc gaacccactc agcaagtttt ccatgcttgc gttcaagtcg cattatgaat
     7801 gagcgggcac actgaaccag taaccggcgt aaatttttat cacctcgctt actgataccc
     7861 aacagagtcg attttcctcc agtgctatac tgccggggaa caagtcctgt ggaagctgca
     7921 aaatccctgc tgcatgagaa ctgtttcccg tcaccaagtt gcgacgatag taagctggca
     7981 gtaatcggac caacaccagg aattgtcata atgcgctgag cggtatcatc agcgttgatt
     8041 gactgactta attctgattc cagctctgct atttgctcaa caaggtaaaa gtagtgggta
     8101 tgcaacctca ccaaaaggcg gctcagatat tcggggattt cgtgttcagc caacacgaga
     8161 gacaaccgtt ttattacagc atcacctatc ggcaggctga ttccaaattc cagcaagaaa
     8221 ccgtggatct ggttggttgt tttcacctta tctctgatga gcgattctct gacccggtgt
     8281 aacgccctca tggcctgctg ggtctcagtt cttggctgga caaaacgcat tgatggtcgt
     8341 gaagccgctt cgcatatggc ttctgcatca acaaaatcat tcttattact tttgacaaac
     8401 gggcgaacga actgtggaga aatcagctta gtttcatgcc caaaatcaga gatacagcga
     8461 gccataaagt gtgcacccgc acaggcttcc attacaacaa ctgagggcgg acatgtcgcc
     8521 agaaactgca tcagtttagt acgggtaaat tttttacgca gaagagcctt accagacttg
     8581 tcttgacaat gaatgtggaa ggagtgcttt ccgagatcga taccaataag tgtgatgttc
     8641 tgcatgatgg tctctccgtt aaagggattt accttaacag cgtactgctg ttaaggtggg
     8701 ggtgaccata tcattatccc caccggtccc atctcaccag attgttgacg ataattttac
     8761 tgccaaattt acaggggcaa ttggttttgt aattccagtt cagccctctg aagacgttga
     8821 tcacccctcg ctcaaacacc tcatccttat tctggtgcag ttgttcaaat gtgctcagaa
     8881 tgttcgcttc gctgatttcc ggaaaattat cgtactccag tgaccggtac catgtatcac
     8941 gggcctgcgt atccattaat gacagcatcc cggaacgttg catcaggtca cgccagattt
     9001 cgcgatcaag attccgggta atggctttca ttgctgtttc gggtttctcc atcagccagc
     9061 agccgcagcg aaaatcctgt ttcatggccc agtccggggc agttttacct ccgatgcttt
     9121 ccgtcagcac cgagatttca gccagttgtc ggataagtgt gtcgatttgc aacagtgtac
     9181 tctttcgtac agccagaatg tggcgaatgc tggttgagca gataacgtcc gtatgcccgt
     9241 tgaccacttc cggacgggag attgtgatat ctgacataat cgatattcct ttttgcagat
     9301 aaatgaaaac gccagccctg tgcaggctgg cgtcagagga atgaagtctg gtgagtgagc
     9361 ttcaccggtt ctggtgcaga agctgcagat gacgcagggt acggacatcc tcccgggcct
     9421 gttcgataca gggcaacagt gctgctgaat ccgttttatc ctcatcgttg tcaaagataa
     9481 ttcccgatcc gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agcgctgttt
     9541 ccgtggttaa tgatttcatg ttgttcctgg ttaccagtcg aaagtgggta aacccggcag
     9601 gcattcagcg ctggcatcca gatgaatgag actgacgcca taacgccgga tgagtgtgac
     9661 gaccagacga cggaacgttt ttgacagccc caggcgttta agccgcagaa ccgggtatga
     9721 ccacgcatcg gtacgtaaca gatatccgct acctgtaaaa tgaatccatt ctgattcacc
     9781 gaagtcactg gtctggtgtg acagcgagta caaccaggcg ttgtccgttt ccgtgatatg
     9841 tgcggtactg cagcgtatgc cggtaagggt agcaaatggt ggcgtgcatt ctgctgaggt
     9901 ctggtcagat tcatcaacaa cacgaaggga gtagccgtta tcgcagacca tgttaatcag
     9961 ttcagcgagc tcaataccat cgacgtcaac gacaatgcgc cctatgttca gtgcctgtac
    10021 gttaacgctg tcggcttcca gtgtcagggc cagtttcatc atttcgcctc cggatactta
    10081 cccagggtaa tgttatttac cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgcccgg
    10141 cgagcccgga ggatatcaat actgtttatt aactgagagc ggggacaggt gctgaatccc
    10201 ggctggtcgg tacgcaccag cgcgtatttt tccacgagaa aattaactgc atcacacagt
    10261 gaaatgcctg cctcaatatg ctgctcaatc acacgttcat cgacaaacgg tgtgtcattc
    10321 agtgtgaggc cataatgctg gtccagcagt cggatgagca gtgtctgcca gatggtgacg
    10381 ggagacgggc agcgagatac ctcccgtaca tgtgtgtcgg ataatgtttt catcttaagt
    10441 tctctgtaac tggtagtgct aaaggtgaga gcttcggggt tatgaggtgg ttgcgggtgc
    10501 tggtgtcgga taaacagcca gataaacgta accacaggag ccgagggtgt cggcttcgca
    10561 ggtcagccct tttgcataca gcgtgacggt atgctgatat cggggattca gttcaccgct
    10621 ggtgagcatg agttccagtt gtttcatcag cagcggaaag gcctggtcca gatggtatgc
    10681 atctgcgtcg ctgaagcggc ctctgatacc ggcgcggtcg gcaaggtaat acaggcggtt
    10741 accctcttgc accagacgag cgccaaaaca gggcctcact gtgcagggaa gcccccacca
    10801 ggggcggtcg tgattgtcat cgggatgagt tatcccggag agtttgtctg acacgataaa
    10861 atcctctctg aaaagtggtt aagaatggtt taacgatgac aggtcagccg gcgcgctgac
    10921 cggtttcggt gaagatgtat gcattgatga gaatggcctc atccacagcg gcgtctgagg
    10981 tcatctggtc gtactcgttt cccgggtgac ggaacatcag ttcagggtga cactcatcag
    11041 ccggacatac gggccaaaac tgtccttacg gcgttctgca aacacggcaa gaacgccggg
    11101 aatatcctgc acttcacgac cagtatacgc ttcagcactg ccgtgccagc gatacttacc
    11161 ggtacagaac ggaaaaagac gggatgcagg atgctgttgg tgaatacgca tggcttcacc
    11221 acgggtgatg attctcatga tgtgatacct ctgaagacag aagataaaag tgaaaacagg
    11281 tgtgatgtgg ttgtgacggg ttaaagcaga ccatgttcgg caaaggagaa aacctggttg
    11341 ccaccgacta tcagatggtc cggcacccgg atatccacca ggcccagtgc ctgtaccaga
    11401 cgttcggtga taagccggtc tgccttactg ggtgtgactt caccggacgg gtgattgtgc
    11461 gccagcacca cggcagcggc attgtggtac agggcgcgtt taatcacttc ccggggatga
    11521 acttccgtac ggttgatggt gccggtgaag agggtttcac cggcaatcag ctgattctgg
    11581 ttgttcagat acagcacccg gaactcttcc cgctccagtc ccgccatgtt cagaatcagc
    11641 cattcccgtg ccgcacgggt ggaggtgaag gccacgccgg gttcatgaag atggcggtcc
    11701 agggtttgca gggcccgcag aatgagactg cgctcgccgg gcgtcatgtt tccgggcaga
    11761 aaggaaagct gctgcattgt ccctgcctcc attcagtcga tgatgcgcat aatggcgctg
    11821 cattccggat gctgcagggc gtaatcccgc agccggtaat aatggaccgt catggcataa
    11881 cactccgtac gacaggcatg atgactgtac gtcatcagac aggcagcaat gccggcggct
    11941 tccgggctca tttcagcgcg gttaccgttc atggcattga acagtaccca tgtttcgtca
    12001 tcatcgtcat ccggttcggg tgccataaat gccccgccgt tgttcagggt gtacagattc
    12061 cagataccac cgcagtagtc ttcacacaga cggtccatcc agccgaagac acggggttcc
    12121 agggtgaccc actgtggaat gaggccaaaa tgctgcggcc agaagctgat gcgctgttca
    12181 tcagggacgg gagtggcaac cagttgttgc aggccagtct ccggtgcaca ggccgaagcg
    12241 gtggtgttct gagacaatgt tttcatgtat gaaatccttg taatgtgaat aaatgagagg
    12301 gagtaaaggt cgggagctgg caacatcacg ttcatcactg accgtgatga cccggtaata
    12361 tcagggatga atgatattcg gctgaaattc cggacagccg tacgcgtaac cgttccggtc
    12421 acagacggat ggccagatgc cattgcctgt atactcagtc tgcacagact gatttctccg
    12481 ggacggtaaa gcagacgggt acaccaggcg cacaggtcat catcagaaat ggaacgacac
    12541 cccggcagcg cctccggggc gtcgggtgat tctggtgcct gaatcacgcc ttcagggaca
    12601 tgctgcctca gcgcaggctc tccagcagcg tttctgccat tacccacaat gcgcggttga
    12661 gcttaatatc ggtgtcgatg ttgtgaatgg cacgggtgtg gatacgtttt ccttttgcac
    12721 tgcggccgga aatcccgcct ttcagcatat tctcctggat ggtctgatat gcgctccaca
    12781 ggtctttacc gtaatcctcc ctgcgtcgtg gtgtcagaat gtcagcggtg gtgactggct
    12841 gatgttcgtc accataacgg taagtcagtg ccgcctgtgc cagcgcctgg cgtgccggtg
    12901 gcggcaggac cagcgactgc atggcatcac gcttctcctc tatccggtca aacacgccca
    12961 ccacctcgta agccccttca ataactttct ccaccacatt tccccggtgt ggaacacgca
    13021 attctcccag agactgaccg cagaccagtc cattggtaca aatggcacga aaatatccgg
    13081 gtaacatctg gtagctggat gaaccgtcat gagagttgag cagaataatt tcagggacat
    13141 gctgaccggt tatctgtccg gcccgccgca gacgcagcat gtgttttgtg tattcccggc
    13201 gacccgggtc acgcacacgt gtctggcagg cgaagaatgg ctgaaagcct tcccgctgca
    13261 ggctttccag tacggtgatg gtgggagtgt acgcatagcg tttactgcgg gaagtatgcc
    13321 ggtcttcacc gaaaatactg ggaacatagt gcatcagttc ttcgtgtgtc agcggacggt
    13381 cacggcgtat ctggtttgca gcataaccaa aacgactggc tagtcgcata atttgctcct
    13441 tatcggtggt taagatttac tggtgtaata aatgaaaaag ccacgtctcc cggagaagac
    13501 gcggcctgat ggacgaaatt aatgaggttt attgtctgag aagaccgtca ctggcgaacc
    13561 ttccggcgtc agtctctgcg ttgcggataa cccgacggaa atgagttccc tggtcatcgt
    13621 cgataaacac attgcggtaa gctgttgtga tgacaacagc ctgctcgcgt gagaaaggga
    13681 cttcgggcaa taaacgtgac atacaaccag acctgtattt ggttccctga aaatataagt
    13741 ccctgtcatc atggcagagc cgtatttact tttgatatca ataaattaat aacgcgaaga
    13801 tgaaccgcat aacttacgcc tttaatttgt tttgtttacg tagtccctga ccaggtacgc
    13861 gaattcgtga acgccagttt caccaaacca aatcaggtaa ccagggtcga tgttgtaata
    13921 gtaaccaacg actttatgcg tttttgggtt atagagtgtt acgatatagt cccagccgca
    13981 ggcgtgggct ttacaaccgc caccttctac ccaggtcgtt ccgttaatgt ttacaggata
    14041 cgcaccacca tcaggaccat ccatatagcc gtcttcccca aacattccgt ttaacccaac
    14101 agggtttaat gctttgtcga tagcagctct gtaggtagcg tcaccttcgt ataactcgta
    14161 cgttgttgga catgcaccag gctggtcagc ccgggctttt gagtcgcatt taagtgtctg
    14221 atttacagtg gcggcgtttg tgtcaggact t
//
LOCUS       DAJVQR010000096        13965 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVQR010000096 DAJVQR010000000
VERSION     DAJVQR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            192..2039
                     /gene="ldtD"
                     /locus_tag="OQM52_003179"
     CDS             192..2039
                     /gene="ldtD"
                     /locus_tag="OQM52_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1564925.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2220..2768
                     /locus_tag="OQM52_003180"
     CDS             2220..2768
                     /locus_tag="OQM52_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1564926.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2795..3442
                     /gene="gloC"
                     /locus_tag="OQM52_003181"
     CDS             2795..3442
                     /gene="gloC"
                     /locus_tag="OQM52_003181"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1564927.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OQM52_003182"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OQM52_003182"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1564928.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OQM52_003183"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OQM52_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1564929.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OQM52_003184"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OQM52_003184"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1564930.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OQM52_003185"
     CDS             complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OQM52_003185"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1564931.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9766..12381
                     /gene="pepN"
                     /locus_tag="OQM52_003186"
     CDS             9766..12381
                     /gene="pepN"
                     /locus_tag="OQM52_003186"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1564932.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OQM52_003187"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OQM52_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1564933.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..>13965)
                     /gene="ssuC"
                     /locus_tag="OQM52_003188"
     CDS             complement(13352..>13965)
                     /gene="ssuC"
                     /locus_tag="OQM52_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCS1564934.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3563 c         3527 g         3324 t
ORIGIN      
        1 ataacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac gtatcggcgt
       61 ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt tcgtgagtat
      121 tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat gataaaaaca
      181 gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca atcagtttgt
      241 gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct gaagtaatcc
      301 ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag gcgcaagcca
      361 cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa gcccgcacgc
      421 aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag cttcaactgt
      481 tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc
      541 agcaacagct ggcagaggtg gcgattgctg gtttccagcc gcagtttaat aaatgggtag
      601 agttactgac cgatcctggt gttaacggga tggcacgtga cgtggtgctc tctgatgcga
      661 tgatgggcta tctccatttc attgcaaata ttccgatcaa aggcactcgc tggctatata
      721 gcagtaaacc ttatgcgctt tcaacgccgc cgctttcggt gattaaccaa tggcagctag
      781 cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat
      841 atgcggcgat gcatgaatcg ttactggtct tactcagtga caccaaaccg tggccccaac
      901 tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg
      961 aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg
     1021 acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta
     1081 agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgtgccgcct
     1141 acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggagcag
     1201 atggtgctat tggcccggca acgcgtgact ggttaaacgt aacgcccgcc cagcgtgctg
     1261 gcgtgttggc actcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga
     1321 tcatggttaa cattccagcc tattcgctgc tctactatca gaacggcaat caggtgctgg
     1381 attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgcactta
     1441 acaacgtagt ggtaaacccg ccgtggaacg taccaccaac tctggcacgc aaagatattc
     1501 tgccgaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg
     1561 gctggaacag cagagaagcg attgacccat gggaggttga ctggtctaca atcacggcct
     1621 cgaatttacc gtttcgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata
     1681 aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt
     1741 tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc
     1801 tggcgaatat gctgttgcag gatgcaggat ggaatgacaa acgtatttct gatgcgctga
     1861 agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc
     1921 tgacggcctt tgttggtgca gatgatcgta cccagtatcg tacagatatt tacaattatg
     1981 atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa
     2041 tgaagtagtt cggggaaatc aattgtcgta ataattagca tgattggggg cgattctctg
     2101 cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt
     2161 gcctcttgtg cgccagaagt gcatataaac gataacattg agctgtagac ttgattatca
     2221 tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg
     2281 ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga
     2341 cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct
     2401 atattcagga agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa
     2461 taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca
     2521 cgcgcaaacc ggtgcaactc atttccggtt atcgttccat tgataccaac aatgaactac
     2581 gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt
     2641 tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag
     2701 gtggtgtagg atattaccca cgtagtaact ttgtgcatat tgataccggg ccagcacggc
     2761 actggtagta atcgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac
     2821 cgcattctcc cagaactgtt cattgatctg gtgtgaacaa acccgcctgg ccgcactggt
     2881 cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact
     2941 gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc
     3001 gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg
     3061 cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga cgccagatcg
     3121 ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg
     3181 ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc
     3241 tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa
     3301 tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat
     3361 tccgggtcac ggaccattat ctacacttgg ttatgagcgc ctgcacaatc ccttcctgca
     3421 agacgaaatg cccgtctggt aaaggcgcat aaaaaagtcc acttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 acgcttacag cactgccaca atcgattcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaacag catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcaga ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcacct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaat gcatcgctgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggagg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacgatttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcgttcag actgttaatc agtgcatcga
     4381 agtcaagggt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacaca cgcttaacgc
     4501 tggtattttt tgccaggaag tcggcagcca cgcgtagtgc gccagtgcca cccggagtct
     4561 gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca aggatgccgt caatgccgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gcataaaaaa aacaggacca acgtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagttttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc cataggaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagagt acgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa agggagccga
     8281 ttagctccct gatgatatta actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc
     8341 agcgcccgaa caaacgcttt atcatggcag atagttttgc cagggctgtc agaaagtttc
     8401 gccaccggtt taccgttaca ctctaccaac ttaatcacaa tattcagggg ttttacctgg
     8461 gggatatcgc aggtcaggcg agtcccaata ccaaaactta attgcacgcg ggaagagaag
     8521 tggcggtata gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac cagcgtttta
     8581 ctctgtggat caattcccag cttttcataa tgtgcaatgg ctttttcacc ccattcaacc
     8641 gggtcgccag agtcatgacg caggccctga taccgactag cgaactcgac accgaaatca
     8701 cgcaggaaag catccatagt gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac
     8761 tcttccagcc aggcagcaag tgcagctcgc tggctgttgg ctagatccgg gctgatttgc
     8821 tgatgtgcct ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc
     8881 gccagatcgt agttgctggt gcccacgaac caggattcct gttgcagacg cttaacgatg
     8941 gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa aatccatcag atggaagcgc
     9001 gacatatcaa gaccggcggt taacgccgag aagtcgacta atttgctttc cagcgtgtcg
     9061 agggcttgcg caacgtcggc ctgcggtgag cgatagcgat gtaccatttc actgataacc
     9121 gccagcaaag gaacttccca gaggatgact tcacgccacg gaccgcttaa gcgaatatcc
     9181 agcttgccat tatcgttgga cacggtgact tgttccgggt taaagcggaa ctcgcgtaac
     9241 cagttaagat agtcggcctt aaagaaaggc agggcagaga gccactgata ttcatcatcc
     9301 tgcaggcgca ggtgctgcat cgcctgaatc tgttcacgaa tagcatcggc ataaataccc
     9361 agcagatcgt cacctcggca acgaaactcc gccgcgacat gcacatcgta atagtgatga
     9421 aacacggctt gctgcatatg caacttataa gcatctgtat ccagcaacga gtgcagaaca
     9481 ggagaagcga attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca
     9541 ataaacatct tcaggaactg ctggagtata ccttgtttcg caatttattg aaccccgatc
     9601 acaccatatg ccacctttct ggtcgatggc attacgcggt gtatgttata aaaatgtagc
     9661 aataaaggcg tttgtacctg aaaagatgaa gattctgcat agcgcgattt acgcaacagg
     9721 aatagactga acaccagact ctataaaaga tgctaaaggt tatttatgac tcaacagcca
     9781 caagccaaat accgtcacga ttatcgtgcg ccggattacc agattactga tattgacttg
     9841 acctttgacc tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat
     9901 ggtgcatcag atgctccgct tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat
     9961 attaatgatg agccgtggac cgcctggaaa gaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcgttttac gcttaagatc attaatgaaa taagcctggc ggcgaatacg
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaatcaa atatcccttc ctgctttcca atggtaaccg cgttgcgcaa
    10261 ggcgaactcg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgatacctt taccacgcgt
    10381 tctggtcgcg aagtagcact ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactccatg aaatgggatg aagaacgctt tggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccggttg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggt aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagcg atcagcaact gaccttcctg atgcgtcatg cgcgtaatgg tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catagacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg taatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggtggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct ttacgagaag
    12361 ataactaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ctaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctgccg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actgaccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtatcatcct gaatttcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac cggccagcag gtgcagcagg
    13201 gtacttttac caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcatatcgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc tgtttgcaaa aactctcgcg
    13501 cattcatcgc cagataacca atgccggaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcatcgg
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatctcca
    13681 ccagcccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaacagcg
    13741 taccgagcgc caccagaaag attttcgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggcacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 tttctcccca gcgcgacagt ccgctaatca gtcccagaat caggcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag atgct
//
LOCUS       DAJVQR010000097        13827 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVQR010000097 DAJVQR010000000
VERSION     DAJVQR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..131
                     /locus_tag="OQM52_003189"
     CDS             <1..131
                     /locus_tag="OQM52_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1564935.1"
                     /translation="LATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            296..4414
                     /gene="pic"
                     /locus_tag="OQM52_003190"
     CDS             296..4414
                     /gene="pic"
                     /locus_tag="OQM52_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCS1564936.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4565..4807)
                     /locus_tag="OQM52_003191"
                     /pseudo
     CDS             complement(4565..4807)
                     /locus_tag="OQM52_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(4846..5796)
                     /locus_tag="OQM52_003192"
                     /pseudo
     CDS             complement(4846..5796)
                     /locus_tag="OQM52_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5847..6422)
                     /locus_tag="OQM52_003193"
                     /pseudo
     CDS             complement(5847..6422)
                     /locus_tag="OQM52_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6426..6826
                     /locus_tag="OQM52_003194"
                     /pseudo
     CDS             6426..6826
                     /locus_tag="OQM52_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7229..7810)
                     /locus_tag="OQM52_003195"
     CDS             complement(7229..7810)
                     /locus_tag="OQM52_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564937.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(7857..11714)
                     /gene="sigA"
                     /locus_tag="OQM52_003196"
     CDS             complement(7857..11714)
                     /gene="sigA"
                     /locus_tag="OQM52_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS1564938.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12075..13079)
                     /locus_tag="OQM52_003197"
                     /pseudo
     CDS             complement(12075..13079)
                     /locus_tag="OQM52_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13064..13441
                     /locus_tag="OQM52_003198"
                     /pseudo
     CDS             13064..13441
                     /locus_tag="OQM52_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13480..>13827)
                     /locus_tag="OQM52_003199"
     CDS             complement(13480..>13827)
                     /locus_tag="OQM52_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1564939.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         3462 a         3251 c         3377 g         3737 t
ORIGIN      
        1 agctggccac actcacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg
       61 gccatatccc accggcagaa gcagaaaaag cttattatgc ttccatcgga aatgatgatc
      121 tggcagcctg agttcacaga taaaacactc tccaggaaac ccggggcggt tcagttcaca
      181 aaaacacatt aatgcagtaa ctatattttc ctttctggtg ataacgtcgg gttatcatta
      241 gcttcttcag ctattttact tttatatccc ttgtaaacat catggagaat ccatagtgaa
      301 taaagtttat tctcttaaat attgccccgt caccgggggg cttattgctg tctctgaact
      361 tgcccgcagg gtaataaaaa agacatgccg aagattaacg catattcttc tggctggcat
      421 tccagcaatc tgtctgtgtt actctcagat atcccaggcg ggtattgtcc gttccgatat
      481 tgcctatcag atttatcgtg atttcgccga aaacaaaggg ctttttgtac ctggtgccaa
      541 tgatattccg gtatatgata aggacggaaa acttgtggga agactgggta aagccccaat
      601 ggccgatttc agcagtgtga gctcaaatgg cgttgctacg cttgtatcgc ctcagtatat
      661 cgtcagcgta aagcataacg gaggatatcg gagtgtgagc tttggtaatg ggaaaaatac
      721 atattccctt gttgaccgta ataaccaccc ttctattgac ttccatgctc cacgtctgaa
      781 taaactggtt acagaagtta ttccctcagc ggtaacatca gaaggaacca aagccaatgc
      841 ttataaatac actgaacgtt acaccgcttt ttatcgggtg ggtagtggta cgcagtacac
      901 taaggacaag gacggaaatt tagttaaggt tgccggtgga tatgctttta aaacaggagg
      961 aaccacagga gttcctctga tatctgatgc aacaatagtc tctaatcccg ggcaaactta
     1021 taatcctgta aacggccctt tacctgacta tggagcccct ggggatagtg gttctccttt
     1081 gtttgcttat gataaacaac aaaaaaaatg ggttattgtt gctgtattaa gagcatatgc
     1141 aggtattaat ggtgctacga actggtggaa tgtcatacca acagattatc tgaaccaggt
     1201 tatgcaggac gatttcgatg cccccgtaga ctttgtttcc ggactgggcc ccctgaactg
     1261 gacatacgac aaaacatcag gcacaggtac cctgagccag ggcagtaaaa actggaccat
     1321 gcacgggcag aaagacaatg acctcaatgc cggtaaaaat ctggtattca gcgggcagaa
     1381 tggtgcaatt atcctgaaag acagtgtgac tcagggtgcc ggttatctcg aatttaaaga
     1441 cagttacacc gtatctgctg aatccggaaa aacatggacg ggtgccggca ttattactga
     1501 caaggggacg aatgtaacct ggaaggtcaa cggcgttgcc ggtgacaact tgcataagct
     1561 gggggaagga accctgacca taaacggaac aggtgtaaac ccgggaggac tgaaaacggg
     1621 agacggtatc gttgtactta accagcaggc agacactgca ggtaatatcc aggccttcag
     1681 ttcagtgaac ctcgccagcg gacgtccgac cgtggtgctc ggggatgccc gtcaggtcaa
     1741 tccggataac atttcatggg gataccgggg aggtaagctt gaccttaatg gtaatgccgt
     1801 taccttcacc cgactgcagg ctgctgatta cggggcggtg attacaaata atgcacagca
     1861 aaaatcccag cttttactgg atcttaaggc tcaggataca aatgtcagtg aaccgacgat
     1921 tggaaatata tccccctttg gtggtaccgg cactccagga aacctgtaca gcatgatact
     1981 caacagccag acccgcttct atattctgaa atctgccagc tatggtaaca ctctgtgggg
     2041 gaacagcctg aatgatccgg ctcagtggga gtttgttggc atgaacaaaa acaaagcagt
     2101 tcagacagta aaagatagga tcctggccgg gcgggcaaaa caacccgtta tctttcatgg
     2161 tcagctgacc gggaatatgg atgtcgccat tccacaggtg ccggggggaa gaaaggtaat
     2221 ctttgatggt agcgtgaacc tgccggaagg taccctgagt caggacagtg gcaccctgat
     2281 attccaggga catccggtta tccatgcctc catcagtggc agtgcaccgg tcagcctgaa
     2341 ccagaaagac tgggaaaacc gtcagtttac aatgaaaaca ctgtcgctga aagacgctga
     2401 cttccatctt tcacgtaacg cctcgctgaa cagtgacatt aagtcggata acagccatat
     2461 cacactggga agtgacaggg catttgtgga taaaaatgac ggaacaggaa attatgtcat
     2521 tccggaggaa ggtacctctg tcccggacac cgtgaatgac aggagccagt atgaagggaa
     2581 tattacgctg aaccataact cagccctgga tatcggcagc aggttcaccg gggggattga
     2641 cgcttatgac agtgccgtca gcatcacctc tccggacgtc ctgttgacag ccccgggtgc
     2701 ttttgccggc agttcactga cagtgcatga tggcggtcat cttacagcac tgaacggtct
     2761 tttcagcgac gggcatattc aggccggtaa gaacggcaaa atcaccctga gcggtacacc
     2821 ggttaaagat acggctaatc agtatgcccc tgctgtatat ctgacggacg gatatgacct
     2881 gaccggcgat aacgcagcac tggaaattac ccgtggagca catgcttccg gtgatattca
     2941 tgcctctgcg gcatcaacag ttaccatcgg gtctgacacg ccggcagaac tggcttctgc
     3001 ggaaacggct gcatcggcgt ttgccggcag tcttcttgag ggctataacg cagcattcaa
     3061 tggtgccata accggtggca gggctgatgt cagtatgcat aatgcactgt ggactctggg
     3121 tggggactct gccatccaca gtcttaccgt cagaaacagc cgtattagtt ctgaaggaga
     3181 ccgtacattc cgtaccctga cggtgaataa actggatgca acaggcagtg attttgtttt
     3241 gcgtacggac ctgaaaaatg ccgataaaat taatgtgact gaaaaagcca ctggttcaga
     3301 taacagcctg aacgtcagct ttatgaataa tcctgctcag ggacaggccc tgaatattcc
     3361 tctggtcacg gcaccggcgg gaacttcagc agagatgttt aaggccggca cccgggtgac
     3421 aggtttcagt cgggtgaccc caaccctgca tgttgatacc agtggtggca atacgaagtg
     3481 gatactggat ggttttaaag cggaggctga taaagccgct gccgcgaagg ctgacagttt
     3541 catgaatgcc gggtataaaa acttcatgac ggaagttaac aatctgaaca aacgtatggg
     3601 tgacctgcgt gacacaaacg gtgatgccgg tgcctgggcg cgcatcatga gtggtgccgg
     3661 ttctgcagac ggtggttaca gtgataatta cacccatgtt caggtcggct ttgacaaaaa
     3721 acatgaactg gacggtgtgg acctgtttac cggtgtcacg atgacctata ccgacagcag
     3781 tgcagacagc catgcattca gcggaaagac gaaatcggtg gggggcggtc tgtatgcttc
     3841 agcattgttt gagtccggtg cctatatcga tttgattggt aaatatattc accatgacaa
     3901 tgattacaca ggtaactttg ctagcctggg aacgaaacac tacaacaccc attcctggta
     3961 tgccggtgct gaaacgggtt accgctatca cctgacagag gacacgttca ttgagccgca
     4021 ggctgaactg gtttacggcg ccgtgtccgg gaaaacattc cgctggaaag acggtgatat
     4081 ggacctgagc atgaagaaca gggacttcag tccgctggtt ggaagaacag gggttgaact
     4141 gggcaagacc ttcagtggta aggactggag tgtgacggcc cgtgccggaa ccagctggca
     4201 gtttgacctg ctgaataatg gagagaccgt actgcgtgat gcgtccgggg agaaacggat
     4261 aaaaggagag aaggacagcc ggatgctgtt taatgttggt atgaatgcgc agataaagga
     4321 caatatgcgc tttggtctgg agtttgagaa gtcagccttt ggtaaatata acgtggataa
     4381 tgcggtaaac gcgaatttcc ggtatatgtt ctgatgtggg ggtaaagggc tgccggaagg
     4441 cagcctttct cagagactct taaaatataa aactgaaggt cttcggtaag ggggagtgta
     4501 ttagacgtca actagttttg ccgactggcg cgagcctgtt taagattctg tgtaaatgcc
     4561 tttctcagaa gtgaccgtcc aggcggtcac cgaactcgat aataaagcgg ctcattgcca
     4621 ttcgccagtc cttcaacggc atcgtccatt tctgggacgc agactggatt gccagccaca
     4681 ccactttttt caccgagtcg tctgtcggga acactttacg ctttttgatc gcatggcgga
     4741 tcacgctgtt tagcgactcg atggcattcg tcgtatagat cactttgcgg atgtccgttg
     4801 gataagcggt aagcgtacag cgagggccgt attgacgggg atgtgttatt cagctggcag
     4861 tgctatgcgc cacggaagca gttcgctgac ccggttgacc ggccagtctg ctatgacgcc
     4921 aagcacatgg cgaaggtagc tttctggatc cacgtcattc agtttgcacg tcccgatcag
     4981 gctgtacagt agcgcgcccc gctcaccacc atggtcagag ccgaagaaca ggaagttttt
     5041 acgacccaga ctgaccgccc gcagggcatt ttcagcgatg ttgttgtcga tttccaccca
     5101 gccattactg cagtacacgt tcagggcctt ccactgtttc agtaggtatg cgaacgcttt
     5161 tgccgtatcc gagtgacgcg acagtgtttt catctgttgc tgtatccggt catacagtga
     5221 ctgcatcagt ggcgcggctc ttgcttttct tgccgccaga cgctgttctg ctgaacatcc
     5281 ccggacttct gcctcgatgg tatacagttc accgatacgc tgcagggctt ccgtggtgat
     5341 gtcggttggc actcttgcat gcacatcgtg gatttttctc cgggcatggg ccatacacgc
     5401 agcttccgtt attctgccgg attcgtataa cgcccggtaa ccaccgtaag catctgcctg
     5461 aagcacaccg ctgtaaccgg ctaggtgatt ctgtggatgg atacctttcc ggtccgggct
     5521 gtacgcgaac cagaccgccg ggggcatctg tgaaccggcg ttacggtcat cacggacata
     5581 gacccacagc cgggcagttc ttgttttacc actgcccggc tcctggaccg gaacggggat
     5641 atcatccgca tgaactttac cgggcatcag tacatactgg cgcaggacgt catacagcgg
     5701 ctccagcagt tcagcaacgg caccggtcca gcgacccagt gtggcacggc tcagctccac
     5761 gccctgacga cggtatattt ctgactggcg gtataactgt caacgacgga tgaaaagtgg
     5821 ctcttccgca acctgggtgg agattttcat gccagtggac tcattaccct ctgcctgagt
     5881 agttcaaatc ctgctcgacc atacatctgg cgtttcagca tcttcttcag gcgatttaca
     5941 tgaccttcga caacaccatt actccagcga ctactgattg cctcacatat agctgcagca
     6001 tcagcttcca tccccgccgc cacgcgccga aattctgctg aaccgctttc gtggactcga
     6061 gtgaaccaac tgcttagctg tgatttattt tgggttttca gaatacggta gaactcgagt
     6121 gccagttgct gcgctatttt cagctcaggt tctttttcac acatcagact aataaatcgg
     6181 gaagcataat tttcttcatc cctggttatt ctccagggca tcaaccagcg gcttacccgg
     6241 gagactgaag gaagtctgac ggcagtagta accggtgggt tccagccttt acgccatttg
     6301 gcaaccgcat ccctgacgat ggtttcactg cctgtaaatc cccgggccac catttcccgc
     6361 catatccggc tggcgttata attaccactt tctcgttgct cttttaacca ctccctccat
     6421 ggtgtcagcg aaaaacgaaa ccgctgttga aatccctgga aagctggttg cggaaaagat
     6481 gaagacccag tcgcgacact cagagttggc gaaggcgttc gcgtacgcac ttaaccagtg
     6541 gccggcactg acgtactatg cgaacgatgg ctgggtggaa atcgacaaca acatcgctga
     6601 aaatgccttg cgggcggtca gtctgggtcg taaaaacttc ctgttcttcg gctctgacca
     6661 tggtggtgag cggggagcgc tactgtacag cctgatcggg acgtgcaaac tgaatgacgt
     6721 ggatccagaa agctaccttc gccatgtgct tggcgtcata gcagactggc cggtcaaccg
     6781 ggtcagcgaa ctgcttccgt ggcgcatagc actgccagct gaataacaca tccccgtcaa
     6841 tacggccctc gctgtacgct tactcatcac ctgctattaa tccccgagtc agtacttcgc
     6901 ggcctgacgc gatcacccta ctcagtactt cagagcccgc ctccggtacc tgcctggcga
     6961 tcgcccctgt gcaacaaaat aaatcaaccg ttatcaaacc acgatcgttc gtatgcgccg
     7021 ccgatgacgc gcgcctgaaa aacgcgtcaa tatgagttcc ccggaagctt acgataatac
     7081 gcttatgaac attacaacac gctcatgcgg aacctggatg ggggaaggaa cggtgaagct
     7141 gttagcgaat gaccggcgtt tatacaaacg ccggtcatga tgaatgatat ttcagaatat
     7201 acctcgggct aaaataccgg gacagagatc aggcgtgatt aatctgcagc atttttaata
     7261 acagaagtac tgagcttact ttacctttga tgatgtaata gttatttccg tcggtactca
     7321 tttttccctg aaataatgtc acggatgttt cttcctgctg atcctgcagg cgcagtcgta
     7381 aggtcatatt tttaatgagc tgagatccgg gtggcaaggg gtatttgctg tcagatactg
     7441 ggcgactgag tctgatgtgg cgcgtataca gcccgttttc gtacagggtg gcctctgtta
     7501 tggtgtcagt gaccagcatg tgggagacaa tgctgtattc ttttttaaag cacccaccag
     7561 aaacggtgat tgtaatgata atggtcttct cgatcgagat gtaaataccc gtttcgttat
     7621 actgtccgta tcggaaccat gagaactcag ttgcattcca gccatgctcc agaatatgtg
     7681 catttttttg cctgatagtt tcttttttta ttcgctcatg tttaaggcat aacaggcaga
     7741 taaataatgt cagaaaaaga aagctaataa tgagagcaag agcgatatag tctgtcacag
     7801 ggtaatacat attctctccg gtagaagaag ggccgcaaac gcggcccggg ctgttatcag
     7861 aaagagtaac ggaagttggc gttgatcgcg ttatccacgt tgtatttacc aaatgccgat
     7921 ttctcaaact caagaccgaa gcgaagatta tcgcgaattt cggcgttgag accaacattc
     7981 atgagaatac gaccgtcttt ttcacctttg atacgtttct caccggacgc atcacgcagt
     8041 acggtttcac cgttggcaaa caggtcaaac tggtagccaa ggccggcgcg ggctgtgact
     8101 ttccagtcct taccggagaa ggatttaccc acatcaacac cggtacgccc aatcagcgga
     8161 ttaaagtcct tatccttcat ggtcacgctc attccgcgat cctgccagtc gaaccgttta
     8221 cctgatacgg ccccataaac cagttctgcc tgcggctcaa tccaggagtc ttctgttaca
     8281 tgatagcggt agcctgcttc agcacccaca tacaaggaat gagaactgta gtcttttgtt
     8341 ccgagtccag caaaggtcgc cgagtactca ttatcatggt gaacatactt actaatcagg
     8401 tcgatatagg caccagagtc aaatatagcg gaagcataca gacctgcccc gacggacttc
     8461 gtcttgccac tgaatgcatt actgctggca tgactgtcgg tatacgtcat agtcagacca
     8521 gtgaaaaggt caagtccatc cagctcatgt tttttatcca caccaatctg cacatgggtg
     8581 tagttgtcac tgtatccacc agaagctgaa cctgctccgc tcatgatgcg ggcccatgca
     8641 ccggcctcgc cattggtgtc acgcagatcc cccattcgtt tattcaggtt gttgacttct
     8701 gtcaggaagg ctttgtaatt accggacata tacgattttg ctgcaccgga agatgctgta
     8761 ttttcagcca ccttatatcc ggtcagggtc cacgtggtat gttcctgatc ctctttcgtg
     8821 ctgatcacag gagatatgtt gctgaaacct atggcctgag tactggcttt aaatatctct
     8881 ttattgcttc cttttggggc tgttatcagg tcaacgttta atcctgagtt attatttccc
     8941 actttctgta tatcgaccga cagactgtta ttgcttccgg ttaactgatt tttaacaatg
     9001 agtttatccg attgatttac agactggctc agatcagccc gcataatcac agcagtatta
     9061 gtggcatcca gtgtgtttac tgtcacagta tggaaacccg aattctctat gttactaaac
     9121 aacaaaagac tgtcgcgggt ttttaatgat tgcagggctg actgtccgtt tactctccat
     9181 aaggtgttat acatgctggc tgaggctgca ggagcatcaa tacctccggt ataagaagta
     9241 tcaaagccac ccagcaaacc tgtagccagc ggtgtgtgtg tggttttgtc caggttatct
     9301 tctttatact ggtcaacacc aaacagaata gatgcttttt tgtctgattt tatattacca
     9361 ttgacccagg cataattttg agcctttaac gtggcaccat cttctgtcat attatatcca
     9421 tctgacatat agattgccgg gctgtattta ttattttctg taggggtgcc tgttaatgtc
     9481 agggttccgg ccagttcaat agctttatct acatacattt ttccctgggc ggtaacagta
     9541 gctcctttgt tgataatcag gtctgtatta attaaggata atgcaccagt catagtaaca
     9601 gttgatttat caccaacagt tattttgctg ttttgagcac tcagactgcc gttgaaacta
     9661 ctttcgcctg ctgaatcccc ggagcgaagc tgttgtctga atccaaaacc ttcgccagta
     9721 atgtttcttc ctgagaacat gtcaatgtat gcagttttat ttcccagaac aatttgagag
     9781 tttttagcct ctatgtgtcc ttttacatta gcatcccggc ctatactcag ggttgcgttt
     9841 tctaaattta atttccggaa attaaattta cgactttccc agtcgggctg ggaaaaagag
     9901 gctatctgat tgttggactt atattccgta ttgtaacgtt tatttacagt gctttcctgg
     9961 tcctttatgg cagcagaata gtctttttga cataaaaaag gaattggaca gttgttattg
    10021 ccttctttaa atatggcatg ttctgtcgca tgtccttgta atgttaactg tgcattccgg
    10081 acagagatat cgtttttgat atcgactgag ccatcaaaga tcagattggc attattgtta
    10141 tgttgctggg tagtattgat aatatgattt atgcttatat tacctgatac attcccatgg
    10201 tacatatatg catcagtatt agtaagtgat aatgttgatt gcttcacgtt tgagttcgtt
    10261 acggttgttc ccgcatctgt tgctgcgatt ttctgaaatg actggtcata gccgtttaga
    10321 tccagtgttc cgccatttct ggtaaaatag atttcgccat taccgctttc gcttaaagcg
    10381 tctttggcat ttatttttac cgtaccttta cctccggcca tgtaaatttt attgaaggct
    10441 ttttcagcac taaggatgac agtcccatta cctattttaa ggttatttcc ctgtgctatt
    10501 tttacatcca gagtaccact acctatttta tgcagattat ctccggcaac gcccttaaca
    10561 ttccattcaa cggtggtatt attatcaata tctatcccgg cacctttata agacttatct
    10621 tttccgtaca ctttatatgt ctggttctta tcgaatataa gcccgccaat gccaagatcg
    10681 gtatcctgct tgagcgttaa tacccctcct cctgagacag acaggtcctt atcttttata
    10741 atcgatgata actcttgttt tttaccatcc agctgcatag tgtttgtgaa attctttttt
    10801 aaattgttta ccagaatatt attaaataac gagtagtttg tccaggctat attggtacta
    10861 ctgataaaac caactgtact ggtaacacca tggaaaaccc atttttgttg ttctttatca
    10921 tatagataag aaccagaacc actatcgcca aaaattggtg agtttttaaa aggggttctg
    10981 ttggtataaa tgtaactctt gtttttccag tccagattaa aaagacttgc gcttaacaag
    11041 tcgggattat agttttgtcc aaacagatat tgtttgccgt ttaatatcgt tgaggttgtt
    11101 ccggcacctg ctctgaagcc aattatttgt tccttaccgt tgtagtttac gccatatctt
    11161 ttcatcgcat cttcgggaga aagtgagaag tcggcactct ccgtaacccc tgttgtttcc
    11221 acgacgaatt tattcagacg atgaaccgaa aagtcgccag atgatactct gttacttgct
    11281 ttatatgctg tctgatccca tgactgtgtt gttattgcat gatgctgtgt accgttatga
    11341 gtcgcagtaa cactgtacgc acctccaatt gatgttgttg cacctttgtt ggaaacagca
    11401 gaaaaatcag gtacaggtag ttctggaaaa tgaaatatct ttccatcttt catcattaat
    11461 tgaacatttg ttgcacccgg tctgaactct cctctgtttt gagcaaggtc aagataatcg
    11521 cgggtccata tattatttat atccagcatt gctgcaaatg ctgtttcgcc ataacttcca
    11581 tatattgagg ataaaataat accgaggata actttctttc ttgatgtacc gacactaact
    11641 ttccgggtca gttcagaaac agcaactaat ccacctgtaa tatgactata tttcagtgaa
    11701 taaattttat tcatacgtga actccgatat ttctgagttg tggttatggg atagcattta
    11761 attacaacgg ggaactatca gtcatggtac gtaactgata agggtataat gataatcact
    11821 gtcagttata tttgtatgac agatgtaaca aaatgaaaaa aaagataagc acaagtgatt
    11881 gattggttag atattgatca cttggtgttt gtattttgaa ttttgcgatc aaaatttgat
    11941 ctttgttgtt tccggtcaat cctttacagg ttttctgtat tatccggggg agttaagtct
    12001 gtcgatgtcc gacagacttt tgatgatgga aacaacatgc agcgataatg tgcatgctgt
    12061 tttgcaggtg gaactcagaa ggtgaactgg aactgtgcga ccgcaccgta agtgttgtcc
    12121 gtgccgatca ggcctgcaac atcaaggtga accacatcaa acggtgagat ccccaccccg
    12181 gccgtgaatg cactgccgtc gttaccggcc agattctgac ggtaaccggc gcgcagctgt
    12241 gcccatttcc aggcattaaa ttctgtgcca atggcggcaa actgacggtt gctgtcggag
    12301 gtgaaaccgc tggccggggt cagatccaca tcaaatgcag tggtgaacat cgcgttgtgc
    12361 caggagacac cggccgtcac ctgcgggcgg atcctgaagg tttccgtgat accgttcact
    12421 tcttttgtct caatactacg ggagatcagg ttctgtgcga ccagtcccag cgtccagttg
    12481 tcatccaggt ccgtactggc accgatgtcg gcgttgatgc catttttcgt gttgtggtag
    12541 cggtcgcctt tgaaggcgct gctgtcataa tttctgacca gcacgttgta gttgaacagg
    12601 tcgacgcgct ggaatttggg ggggaagccc agagaccatt tctgcccggc cgtttccagt
    12661 tccgtagcca gagcaatacc cacatcagtg atcagtgccg tgcgggcaca ggcctgtgat
    12721 gtcaggcggc ttttgtccac ctcctgcccg gtgctgtttg ccacctcttc caggtaagtg
    12781 aggtcagcat cagagacttt accgtcgaca ctgacggtgc cgtgtgattt aaccatcagt
    12841 gcggcagaga ggcgatcacc gggaagggcc gccacagcag aaacgcccag ctgtgcggcg
    12901 gcatgtgtgt tacggaagtc ccggaggtgt tctttcaggc gtgcggcagc ctcaggaaca
    12961 ccgtggttac tttcgatggc gcggtcgaag gccttccact catcactgat ccggtcggcg
    13021 ttgtcggtga tattatccgg attggagagc tgcgcgccga cggacggaag caggatgctg
    13081 ggctcatgcg cgacgcaaaa tccacgatgt atatatcagt actaaaagcg cgacggcgga
    13141 agaagctctg aaactaatcg gtgaactgta cgccatagag cacgatatac gcgggttacc
    13201 ggtgtctgaa cgcctggcgg tcaggcaaat gcagagtaga ccgctcctga cttccctgta
    13261 taagctgatg caggagaaag aacacacgtt atcgaagaaa tgtcgtctgg gagatgcgtt
    13321 ccggtatatc aggaagcact gggctgcgtt gtgcaacttc tgtgatgatg gcctggctga
    13381 ggtagataat aatgcagcag aaagagcgct tcgtgcagtc tgtctcggga agaaaacttt
    13441 ttcgaggatc actcattccg tgtctgactg cttcagggtt taattttgct tctgctgact
    13501 gaatgatgat tgggcagcaa gtgcaatatc ccacataaat ccagtcaact cacgagccac
    13561 cgccgtaatg gctacttgtg attttttccc ttttgctgtc agagttcggt aacgagaaca
    13621 caatcgttgt tgagctttcc atgcaatatc tttggaatat tgtgttacag agtccggtcg
    13681 gtaatatata agccatgctc caacttttgc aggtgtacga taagaccagg cggcttcgta
    13741 aagcagacgt ctcagttcgc tgtttccaac ttttgttatt cctctgggac gaatactgtt
    13801 tccactggaa tattcacctg gcaccag
//
LOCUS       DAJVQR010000098        13240 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVQR010000098 DAJVQR010000000
VERSION     DAJVQR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(7..989)
                     /gene="kdgT"
                     /locus_tag="OQM52_003200"
                     /pseudo
     CDS             complement(7..989)
                     /gene="kdgT"
                     /locus_tag="OQM52_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1249..1869)
                     /gene="sodA"
                     /locus_tag="OQM52_003201"
     CDS             complement(1249..1869)
                     /gene="sodA"
                     /locus_tag="OQM52_003201"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1564940.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2154..3188
                     /gene="rhaT"
                     /locus_tag="OQM52_003202"
                     /pseudo
     CDS             2154..3188
                     /gene="rhaT"
                     /locus_tag="OQM52_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3185..4051)
                     /gene="rhaR"
                     /locus_tag="OQM52_003203"
     CDS             complement(3185..4051)
                     /gene="rhaR"
                     /locus_tag="OQM52_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1564941.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4107..4943)
                     /gene="rhaS"
                     /locus_tag="OQM52_003204"
     CDS             complement(4107..4943)
                     /gene="rhaS"
                     /locus_tag="OQM52_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1564942.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5231..6700
                     /gene="rhaB"
                     /locus_tag="OQM52_003205"
     CDS             5231..6700
                     /gene="rhaB"
                     /locus_tag="OQM52_003205"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1564943.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6697..7956
                     /gene="rhaA"
                     /locus_tag="OQM52_003206"
     CDS             6697..7956
                     /gene="rhaA"
                     /locus_tag="OQM52_003206"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1564944.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8198..9022
                     /gene="rhaD"
                     /locus_tag="OQM52_003207"
                     /pseudo
     CDS             8198..9022
                     /gene="rhaD"
                     /locus_tag="OQM52_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9032..9346
                     /gene="rhaM"
                     /locus_tag="OQM52_003208"
     CDS             9032..9346
                     /gene="rhaM"
                     /locus_tag="OQM52_003208"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1564945.1"
                     /translation="MIRKTFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9647..10093
                     /locus_tag="OQM52_003209"
     CDS             9647..10093
                     /locus_tag="OQM52_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1564946.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10104..11555
                     /locus_tag="OQM52_003210"
     CDS             10104..11555
                     /locus_tag="OQM52_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCS1564947.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11545..12615
                     /locus_tag="OQM52_003211"
     CDS             11545..12615
                     /locus_tag="OQM52_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1564948.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12615..>13240
                     /locus_tag="OQM52_003212"
     CDS             12615..>13240
                     /locus_tag="OQM52_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCS1564949.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3155 a         3458 c         3488 g         3139 t
ORIGIN      
        1 gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt tgactttacg
       61 tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg ccgtcgctac
      121 cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg caatcagcac
      181 aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag tgccatcgcc
      241 accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa ttactgccac
      301 acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa tggtattgcc
      361 cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa attctcgtaa
      421 ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg cgccgacgaa
      481 aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg tcatgagcgg
      541 cccggactcc aacgacatca acacaaatgc ccagcttctt ctttcgtacc gtactgctgc
      601 atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccagcgtt
      661 gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt
      721 gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt
      781 accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga
      841 atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc
      901 ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg
      961 attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc
     1021 tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt
     1081 aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt tataggacca
     1141 ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt
     1201 taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc
     1261 cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc
     1321 cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat
     1381 cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt
     1441 agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg
     1501 ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc
     1561 gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag
     1621 gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag
     1681 ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc
     1741 cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat
     1801 ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt
     1861 atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt
     1921 tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt acttttcgta
     1981 agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt
     2041 gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt
     2101 tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta
     2161 acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt
     2221 acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga
     2281 ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg gatttctggg
     2341 cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt
     2401 gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg tcgatgggaa
     2461 ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg
     2521 gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc ggcgttctgg
     2581 tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg
     2641 gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca
     2701 ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg
     2761 ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc atcatgggcg
     2821 gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt
     2881 cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg
     2941 cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc cacgcccgca
     3001 ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg
     3061 gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg
     3121 tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg
     3181 cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc ccggtttccc
     3241 gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc gaaatatcac
     3301 tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg acctgccgca
     3361 gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac
     3421 tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg ctagccgcca
     3481 gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac gaatcactgg
     3541 tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca gccatttcgt
     3601 tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc tgccgcgcct
     3661 gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg
     3721 gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa ataatattct
     3781 gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga atataaaaga
     3841 gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca ttaccgcgcc
     3901 agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct tgcggataac
     3961 ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg agaagtttta
     4021 actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc gcaatatggc
     4081 gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc ctggcgaata
     4141 tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg
     4201 aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag tagatgtcgg
     4261 gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt ttgctgctta
     4321 agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc atcccaattc
     4381 acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg tgatgcgctg
     4441 ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg cataaacaag
     4501 atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca
     4561 accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga cggatactgc
     4621 ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc
     4681 gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag atgccgatca
     4741 tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc attaaacaca
     4801 tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga
     4861 aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga cggaaaaaaa
     4921 tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta
     4981 atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat
     5041 ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca ccacaattca
     5101 gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt
     5161 cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg aaattcagca
     5221 ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg
     5281 cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg
     5341 ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg atagcctgga
     5401 aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta tcgatagcat
     5461 tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc agcgtgtggg
     5521 cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg cacaacagca
     5581 actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct tcaatacgct
     5641 ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca ttgctcacgc
     5701 tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact gggaatatac
     5761 caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg agtcgctact
     5821 ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat
     5881 tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg ccagccatga
     5941 taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt atctctcttc
     6001 tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg acacggcgct
     6061 ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat
     6121 tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg atctcccggc
     6181 gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc ccaatgacga
     6241 tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc gggaaacggc
     6301 gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca gtctggcgct
     6361 gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt tctcgcaact
     6421 gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg ccgatgcctg
     6481 cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata tcggcatcga
     6541 gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg tcagcaccac
     6601 cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact atgtggcgca
     6661 gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact ggaacaggcc
     6721 tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga ggcgctgcgc
     6781 caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt
     6841 gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta tccgggcaaa
     6901 gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct gattccgggg
     6961 ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt ctcgcgcgac
     7021 cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa tcagctcggt
     7081 ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt tacgctctcg
     7141 catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag ccgtcgcgtt
     7201 tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg gatcccggat
     7261 ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct ggcagcactg
     7321 gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt tgagagcaaa
     7381 ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta catggggtat
     7441 gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc gactgaagtg
     7501 atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct gcacgtcagc
     7561 cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac ccaggcaatt
     7621 gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct tgacttcttc
     7681 gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc
     7741 ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc ggcgggcgat
     7801 tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca ggcggtctgg
     7861 gaaatgtatt gccaacatca cgatacgcca gcaggtagcg aatggctgga gagcgtgcgg
     7921 gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc ggcacgagcg
     7981 ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc cggccctctc
     8041 cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga acaccgctcc
     8101 ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag ccgcaccatg
     8161 tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta cccagtcctg
     8221 gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct gggacgagcg
     8281 caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt atcacgacaa
     8341 cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac tggcaaatac
     8401 gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg atcctgcagc
     8461 taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc tctgggggtt
     8521 aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc actgcgagcg
     8581 cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca ccaacctgat
     8641 cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac tgtgggaagg
     8701 cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt ggatggtgcc
     8761 cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt cgctggtgtt
     8821 gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct ttggtttaat
     8881 cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg gcggcatgaa
     8941 acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg ttacaccact
     9001 agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaaacctttg tcatgcaggt
     9061 aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc cggaactgga
     9121 agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca aagcgcgtaa
     9181 tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg tagccagtac
     9241 tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta acccagataa
     9301 cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc ccaatgtgcc
     9361 ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca tattacatct
     9421 cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa tgaataaccc
     9481 attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg caaatctgac
     9541 ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat cacttaatcg
     9601 ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg cagctcttac
     9661 tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc tgaagcagtt
     9721 ggcgacaata gcgttacaaa acggttttat caccgactca caccagttcc tgcaaaccct
     9781 gctgctgcgc gaaaaaatgc actctactgg atttggttcc ggtgtcgcag tgccgcacgg
     9841 taaaagcgcc tgcgttaaac aaccgttcgt attattcgcc cgcaaagcgc aggctattga
     9901 ctggaaagcc agcgatggcg aagacgtcaa ttgctggatc tgcctcggcg tgccgcaaag
     9961 cggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta ttcaccagga
    10021 atttattcat caactgcaac agggcgatac cgaccaggtg ctggccttgt taaatcaaac
    10081 cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg atcaccaact
    10141 gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag aaagcccgtt
    10201 ctctcggcca taccataaaa gtggaaactc aagggtccag tggcgttgaa aaccgcttat
    10261 ccagcgaaga gattgccgct gccgattacg tcattcttgc taccgggcgt ggcctgagcg
    10321 gtgatgatcg cgcgcggttt gccgggaaga aagtttatga gattgccatc tccctggcgt
    10381 tgaaaaatat cgaccagatt ttcagcgaat taccgacaaa ctcgcagctt tttgccgcag
    10441 atagcggcgt gaagcttggc aaacaggaag tgcaaagcgg cagcgtgatg agccacctga
    10501 tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg gtggcgcttg
    10561 ccaacatgct ggtgcagttc ggtttgccgt ataccgatat gtcgaaaggc gcgccgtcgt
    10621 ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc cccatcatgg
    10681 gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg tttctggttt
    10741 gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga ttcctcggcg
    10801 cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa gtgcgccttg
    10861 gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact ctgctggttt
    10921 tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg ggcgggctgc
    10981 tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt ctggtcggtg
    11041 cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc ttctgcttct
    11101 cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc gcgttgatgc
    11161 cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc cgacaggaaa
    11221 aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa ccggctatac
    11281 cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg ggcggcatta
    11341 ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg ggtatctttg
    11401 acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg gcgattggcc
    11461 tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc cgcaaagcga
    11521 aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt gtgcgaagcc
    11581 agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac gctggaacct
    11641 tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa gggaaataaa
    11701 ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt cacccacatc
    11761 gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca gtcgatgctc
    11821 aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctggtgt gattggtttc
    11881 gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt tgatgagatg
    11941 ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt ggcgatgggc
    12001 gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt ggtcggcaag
    12061 gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac ggtgaataat
    12121 cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct acgcggggca
    12181 caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac cgctgtcgca
    12241 ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca ggggccgggg
    12301 ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc gttaaaaaac
    12361 tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg agcgacggat
    12421 ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg cctgccaacc
    12481 cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc tctgctcacg
    12541 ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt tagccagttc
    12601 cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg ctggagcaac
    12661 agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg accatcctgc
    12721 gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc gccagtggca
    12781 gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg ctgcaaaagc
    12841 acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact ccccgtgcgc
    12901 aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg ccccagttaa
    12961 aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc catttcattg
    13021 atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa gatccgtttt
    13081 taattccgct ggcgggcata acacgagaca accttcagca tttatccacg gcctgcgaca
    13141 accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt ctggcgattt
    13201 acgccctgcg taatcaactg accgatgagt ggccgcaata
//
LOCUS       DAJVQR010000099        13159 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVQR010000099 DAJVQR010000000
VERSION     DAJVQR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..890)
                     /locus_tag="OQM52_003213"
     CDS             complement(<1..890)
                     /locus_tag="OQM52_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1564950.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OQM52_003214"
     CDS             1131..1931
                     /locus_tag="OQM52_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1564951.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OQM52_003215"
     CDS             2009..2779
                     /locus_tag="OQM52_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1564952.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OQM52_003216"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OQM52_003216"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1564953.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OQM52_003217"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OQM52_003217"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1564954.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="OQM52_003218"
     CDS             5034..5756
                     /locus_tag="OQM52_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1564955.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OQM52_003219"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OQM52_003219"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1564956.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="OQM52_003220"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="OQM52_003220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1564957.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="OQM52_003221"
     tRNA            7149..7225
                     /locus_tag="OQM52_003221"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="OQM52_003222"
     CDS             complement(7356..8402)
                     /locus_tag="OQM52_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCS1564958.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OQM52_003223"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OQM52_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OQM52_003224"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OQM52_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS1564959.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OQM52_003225"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OQM52_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS1564960.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="OQM52_003226"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="OQM52_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OQM52_003227"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OQM52_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3026 a         3408 c         3493 g         3232 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgtat agactggccc cctgaatctc cagacaacca gtatcactta
    13141 tttaagtgat agtcttaat
//
LOCUS       DAJVQR010000100        12908 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVQR010000100 DAJVQR010000000
VERSION     DAJVQR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OQM52_003228"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OQM52_003228"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1564961.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OQM52_003229"
     CDS             complement(1806..3224)
                     /locus_tag="OQM52_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1564962.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OQM52_003230"
     CDS             complement(3378..3722)
                     /locus_tag="OQM52_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1564963.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OQM52_003231"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OQM52_003231"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1564964.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OQM52_003232"
     CDS             complement(4712..5647)
                     /locus_tag="OQM52_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCS1564965.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEKNALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OQM52_003233"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OQM52_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS1564966.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OQM52_003234"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OQM52_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1564967.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OQM52_003235"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OQM52_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1564968.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OQM52_003236"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OQM52_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1564969.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OQM52_003237"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OQM52_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1564970.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OQM52_003238"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OQM52_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1564971.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OQM52_003239"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OQM52_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCS1564972.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OQM52_003240"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OQM52_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCS1564973.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OQM52_003241"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OQM52_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCS1564974.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3401 a         3298 c         2950 g         3259 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct tttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAJVQR010000101        12744 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVQR010000101 DAJVQR010000000
VERSION     DAJVQR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..405
                     /gene="torD"
                     /locus_tag="OQM52_003242"
     CDS             <1..405
                     /gene="torD"
                     /locus_tag="OQM52_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1564975.1"
                     /translation="VRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLV
                     EAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQWLPE
                     FAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(498..803)
                     /gene="cbpM"
                     /locus_tag="OQM52_003243"
     CDS             complement(498..803)
                     /gene="cbpM"
                     /locus_tag="OQM52_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1564976.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(803..1723)
                     /gene="cbpA"
                     /locus_tag="OQM52_003244"
     CDS             complement(803..1723)
                     /gene="cbpA"
                     /locus_tag="OQM52_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1564977.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1984..3240
                     /locus_tag="OQM52_003245"
     CDS             1984..3240
                     /locus_tag="OQM52_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCS1564978.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3533..4774
                     /gene="agp"
                     /locus_tag="OQM52_003246"
     CDS             3533..4774
                     /gene="agp"
                     /locus_tag="OQM52_003246"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS1564979.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4812..5039)
                     /locus_tag="OQM52_003247"
     CDS             complement(4812..5039)
                     /locus_tag="OQM52_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS1564980.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5060..5656)
                     /gene="wrbA"
                     /locus_tag="OQM52_003248"
     CDS             complement(5060..5656)
                     /gene="wrbA"
                     /locus_tag="OQM52_003248"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1564981.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6029..6136
                     /locus_tag="OQM52_003249"
     CDS             6029..6136
                     /locus_tag="OQM52_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1564982.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(6218..7546)
                     /gene="rutG"
                     /locus_tag="OQM52_003250"
     CDS             complement(6218..7546)
                     /gene="rutG"
                     /locus_tag="OQM52_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS1564983.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGMMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7567..8061)
                     /gene="rutF"
                     /locus_tag="OQM52_003251"
     CDS             complement(7567..8061)
                     /gene="rutF"
                     /locus_tag="OQM52_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS1564984.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(8072..8662)
                     /locus_tag="OQM52_003252"
     CDS             complement(8072..8662)
                     /locus_tag="OQM52_003252"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS1564985.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8672..9472)
                     /gene="rutD"
                     /locus_tag="OQM52_003253"
     CDS             complement(8672..9472)
                     /gene="rutD"
                     /locus_tag="OQM52_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS1564986.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(9480..9866)
                     /gene="rutC"
                     /locus_tag="OQM52_003254"
     CDS             complement(9480..9866)
                     /gene="rutC"
                     /locus_tag="OQM52_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1564987.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9878..10570)
                     /gene="rutB"
                     /locus_tag="OQM52_003255"
     CDS             complement(9878..10570)
                     /gene="rutB"
                     /locus_tag="OQM52_003255"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS1564988.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(10570..11661)
                     /gene="rutA"
                     /locus_tag="OQM52_003256"
     CDS             complement(10570..11661)
                     /gene="rutA"
                     /locus_tag="OQM52_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCS1564989.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            11949..12587
                     /gene="rutR"
                     /locus_tag="OQM52_003257"
     CDS             11949..12587
                     /gene="rutR"
                     /locus_tag="OQM52_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS1564990.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         3251 a         3207 c         3246 g         3040 t
ORIGIN      
        1 gtacgtgacg atgcccgtct ggaactggcc gctgactttt gcggcctgtt tctgatgacc
       61 gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac aggacgagca agagattaaa
      121 cgcttgttag ttgaggcagg gatggaaacc agtggcaatt tcaacgaacc ggcagatcat
      181 ctggcgatct atctcgagtt gttgagttat ctgcattttt cgctgggaga ggggaccgtt
      241 cctgcgcgaa gaatcgacag tttgcggcaa aaaacactga cggcgctgcg gcaatggtta
      301 ccagagtttg ctgcgcgttg tcgacagtat gacagctttg gtttttacgc ggcactaagc
      361 cagttattgc tggtgttagt ggagtgcgac caccaaaaca gataacgtcg tttatgcgcc
      421 tgaaaagacg cgtttagcgt cgcatcaggc attatggcgc agttgccgga tgcgtcatca
      481 catccggcaa acacacatca cggatgagct acaaaccggg aaagccgctg gcgtagcagg
      541 cggttttcct gcttcaggtg cgcaatatca tccattaacg tcagcgccac cgcgatcccc
      601 ggccagtcca gagccagttc atgacgcagg cgtaccgcgc gttgcaccac aatggcggca
      661 tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg gttcaaccac ccccaaaccg
      721 acaatttcat tcaactcctc ttcagagatg ccggtatgca ggcaaaattc ggtaatagta
      781 aaagtcaccg taacattagc cattatgctt tcccccaatc tttacgtgga tcaaaagacg
      841 actgggcgtc tgccagttgc tgccacagcg cggcagtgtt ttcatccggt ttcggcggca
      901 tcacgatttt cagtaccgca tacagatcgc cggtctgttt tttgctcacc agacctttgc
      961 ctttaacgcg caatcgttgc ccggcctggc tgcctggcgg gatagtcagc aaaatgcttt
     1021 ctttcagtgt tggaacggtg actttagtac ccagcgccgc ttcccacggg ctaaccggca
     1081 ccacaatttc cagatcctgg ccgacaatat cgaacagcgg atgtggcgca atatgaatca
     1141 ccagccacaa atcgccattt ggaccgccgt tttcgcccgg cgtcccctgg cctttcaggc
     1201 ggatgcgttg accattgccg acgcctgccg ggatcttcac attcagcgtt ttcgggattt
     1261 cctgttcgat catgccaaag gcgttataaa ccggcaggtt atagctgatg gtacgcttat
     1321 gctcagtaag cgtttcttcg aggaataccg ccacttcgat ttcaatatcg tggccacgtg
     1381 cggcggggcg ttgacggctc tggcgggcat gctgaccgaa aattgacgag aagatatcgt
     1441 caaaatcttc ggcattaaaa ctctgaccgt cgccatgatg gaactgacgg ttaaattgcg
     1501 gatcgttgcg atgttgccac atctgatcat actcagcgcg acgttgttca tcacttaaca
     1561 cttcccaggc ttcagcgacc tctttgaagc gggcttcggc atccggttct ttgctgacat
     1621 caggatggta tttacgggcc agtcgacgat aggcggtctt gattgtcttg agatcgtccg
     1681 tcggtttcac gcccatgatg gcgtaataat cctttaattc catagcgtta tctcgcgtaa
     1741 atcaacacaa attgaaggaa cccctgtaag gtaactccta taagtgtagg gtaatcctca
     1801 aaatttcata tgccaacaca gaatatgtta ttgaaatcat cgcggagagg aggtcgccat
     1861 caagatgggt tgctgaacat attttaaaca ggtgaaaaag ggtgagcgat ttttgatagt
     1921 tgaaccaggc actttaagtt taactagggc gtcattattt attaaatttt atagacgcta
     1981 tatatgggta gtaatataca tggaattagt tgcactgcaa ataattattt gaaacaggcc
     2041 tggaacgata taaaaaatga gtacgaaaaa aatcaaacat attcaatcac gctttttgaa
     2101 aacacactgg tgtgttttat gcggttgtac aatgaactca gacgtaaagt aaatgaagag
     2161 gatactccat gtctggaatg tgaatcacta gaaaaagaat ttgaggaaat gcagaatgat
     2221 aatgatctat cattatttat gagaacattg cgtactaatg atacacaaat ttattcaggg
     2281 gtttcaggag gtattacata tactatacaa tatgttcaag atgttgatat tgttagagtg
     2341 tccttgccgg gcagaggttc agagtctatc acagatttta aaggttatta ttggtatggc
     2401 tttatggaat atattgaaaa cattaatgcg tgtgatgatg ttttttctga gtattgttta
     2461 gataatgaaa atatgagtat ccagccagaa cagataaata tgccgggaat atctgattta
     2521 gatactggta ttgatttgtc tggcatatct tttattcaga gtgaaattaa taaaacatat
     2581 ggattaaaat acgctccagt agatggcgat ggatattgtc tgttaagagc catactggtt
     2641 ttaaaagaac atgagtattc atgggcgctg ggcagccata agacgcaaaa gcaagtatac
     2701 gaagagttta ttaaaattgt tgataaacaa acgatcgagg ctcttgttga tacggcattc
     2761 tataatctca gggaagatgt aaagacatta tttggcgttg atctgcaatc tgacaacaaa
     2821 attcagggtg tgggtagttt tatgtcatgg agctttctgt tttttaaaaa acaattcatt
     2881 gatagttgct tgaatgacaa aaaatgcata ctgcatttac ccgagtttat atttaatgat
     2941 aacaagaatt tgcttgcttt agataccgac acgtcggata ggattaaagc ggtgaagaat
     3001 tttcttgctg ttctttcaga tagcatttgc tcattattta ttgttaatag taatgtggca
     3061 tcaatctcct tgggaaatga atccttttca acagatgaag atcttgagta tggatattta
     3121 acaaacactg gcaatcatta tgatgtttac ctccctcctg aacttttttc tcaggcttat
     3181 aagttaaaaa ataaggaaat gaatgcgcaa cttgactatt taaatcgtta tgcaacttaa
     3241 tggcaaaggc atatgctaaa aaccattgtt attagtctca cactttttta ttggtaaata
     3301 ttgtctctgt attggtaacg ccgcagatat tctgtttagc cacaggtgca attatcagcg
     3361 gcgtacgcga ggcaggggct aatcaggcat agtttgcgtc aaaccttgcc tgtttttgaa
     3421 gatgtatata gaaaaacagg cgttcaacaa gccattttgc gaacctgttc ccggaaaaaa
     3481 gtcatatttc tgtcacactc tttaatgatt gataacaaaa gaggtgccag gaatgaacaa
     3541 aacgctaatc gccgcaactg tggcagggat agttttactc gcttcaaatg ctcaggcaca
     3601 aaccgtaccg gaaggctatc agctacagca agtgctcatg atgagccgcc ataacttacg
     3661 tgcgccgctg gcgaacaatg gcagtgtgct ggagcagtcg acgccgaata aatggcccga
     3721 atgggacgtc cccggtgggc aactcaccac caaaggtggc gtgctcgaag tgtatatggg
     3781 ccattacatg cgtgaatggc tggcagagca ggggatagtg aaatcggggg aatgcccgcc
     3841 gccggacacc gtttatgcct atgccaatag tctgcaacgt accgtcgcta ccgcccagtt
     3901 ctttattact ggtgcattcc ctgggtgtga tattcctgtg catcaccagg aaaaaatggg
     3961 cactatggac ccaaccttta acccgatgat caccgatgat tccgccgcat tcagtgaaca
     4021 ggcggtggcg gcaatggaga aagagctcag caaactccag cttaccgaca gctaccagct
     4081 actggaaaaa atcgttaact ataaagattc ccctgcctgt aaagagaaac aacagtgttc
     4141 gctggtggat ggcaaaaata cctttagcgc caagtatcaa caagaaccag gtgtttccgg
     4201 gccgctgaaa gtcggcaact cgctggtaga tgcgtttact ttgcaatact acgaaggttt
     4261 tccgatggat caggtggcct ggggagaaat caaatctgac cagcagtgga aggtattgtc
     4321 gaagctgaaa aacggttacc aggacagtct gtttacctca ccggaagtgg cgcgcaatgt
     4381 tgcgaaaccg ctggtcagtt atatcgacaa agccctggtc accgatcgcg ccagtgctcc
     4441 gaaaatcacg gtgttagttg ggcacgactc caacatagcc tctctgttaa cggcgctgga
     4501 tttcaaaccg tatcagttgc atgaccagaa cgaacgcacg ccgattggcg gcaaaatcgt
     4561 tttccagcgt tggcatgaca gcaaagccaa tcgcgatttg atgaaaattg aatatgtgta
     4621 ccagagtgcg gaacagttac gtaatgccga tgcgttaacc ctgcaggcac ctgcgcagcg
     4681 cgtgacgctg gaattaagcg gttgcccgat agatgccaac ggtttctgcc cgatggataa
     4741 gtttgatagc gtgttgaatg aggcggtgaa ataacagaaa actcccccgc gagaagcggg
     4801 ggagtcgctg gttaaacgtt tttacgttcg atggtctgtt cgccccaaaa aagcgaatct
     4861 ttatcggtct tagcaaaggc tttgactaac acttcatcac taccttcttc ccaaatcttt
     4921 tctgccattt tttcgtcgta cccggcgact tcgaaaatgg cctcggctat ttccggcgac
     4981 gtattgcgca gagatgccca ttcaccgacg tgatgagctt tcgcttcttg agttggcatg
     5041 cgtatcctcc tgttgaagat tagccgttaa gtttaactgc cagacctgcg acatattccc
     5101 cttgataacg agcaatagac agttcttcct ggcttggctg gcgtgagccg tcaccgcctg
     5161 cgatggtggt tgcgccgtac ggcgtaccgc cgcgaacctg tgaaacgtca aataattcct
     5221 gcgctgcgta gccaatgggg acaatcacca tgccgtgatg cgcaagggtc gtccaggtgg
     5281 atgtgatggt ttgttcctga ccgccgccag taccggtgga actaaagacg ctcgccagtt
     5341 ttccgtatag tgcgccggaa gcccacaggc cgcccgtctg gtcgaggaag gtacgcattt
     5401 gaccggacat gttgccaaag cgggtaggtg taccaaaaat aatggcgtcg taatcggcca
     5461 gttcttgcgg ggttgcaacc ggtgcagttt gcgttttacc gcctgctttt tcaaataatt
     5521 gcggcggcat ggtttccggt acacgcttaa cgacaacttc tgcgccatcc actttgcttg
     5581 caccctcagc gactgcgcgt gccatcgttt caatatgtcc gtacatggaa taataaagca
     5641 ccagaacttt agccatttct aaccactcct cgtgttatct ctattccgta gcgattcgct
     5701 accacttatt taaagataag acgtcctttt cagagtgcaa atttcacaac cacttatttg
     5761 atttataaca actttcacaa gcacgtaatt ttgtcgcaaa atgacacatt tttatctcat
     5821 cgcgtttttt taatcataag agcggcttat ggataattat tggagatgat atctattctc
     5881 gctaagaagc tgttgcagga tattaccaaa cgcgggtctg tccgcgtcag ttcactaagc
     5941 ttagtcccac gtagcgaaaa tatggcagcc gccatacgcc gcgttaattc tatgcaatat
     6001 gatgtctata cccagacgga ggtcagtaat ggcaaaccat cgaggcggtt ccggcaattt
     6061 tgcagaagac cgcgaaagag catcagaagc aggtaaaaaa ggtggcaaga gcagtcacgg
     6121 caaaagcgac aactagccgg gctaatcaat gacgaatgca tttttttctt aagctcgtca
     6181 aaaagtcatc accgccggta acccggcggt tttctgttta tggctcctga tgaacaactt
     6241 ctggcggtgg aacgtcaacc aaccttctgc ttaataacgc attgagtaaa atcgcgccaa
     6301 aggttgctgt accgatccct cccaacgtaa aaccgccaag cgtgagagca aaatcacccg
     6361 cgcccagcac gaaggttact gcgaccataa tcaaattagc gttctggctt aaatcgacac
     6421 ggttttgtac ccatatcctt gcgcctgcga cggcaatcag cccgaacaca acaattgatg
     6481 caccaccaat caccgcggcc ggaatggtat ggatcagcgc gccaaatttc ggcgaaaagc
     6541 ccaacagcat agcgatgacg gcagctgcaa caaacaccag cgtcgagtag acttttgtca
     6601 cggccatcac accgatattt tcagcatagg tggtgacgcc gctaccgccg acagagccgg
     6661 aaagcatcgt tgccagacca tcaccgacga atgctcgccc catatacggg tccatattgc
     6721 gtccggtcat cccggcgacc gccttgagat gacctaagtt ttccgccacc agaatcaccg
     6781 ccacgggcgc aatcaacatc atcgcctggc cattaaaacc aggagtggaa aaatgcggca
     6841 gaccgaacca ggcagcatag ctgacgagag taaaatcgac ggcttttccc agccctaaaa
     6901 cgttggtcat catgccatac agcagacagg cgacaattaa tcctacgaga atcaataacc
     6961 gctggatcat gccacgggta aacaccgcca ccagcccaat acacagcacc gtcattaccg
     7021 ccatccagct atcaaaggcc gaagccgata cacttttcac tgcgataggc gctaagttca
     7081 ggccaatcgc catcaccacc gcacccgtca ccaccggcgg cattagtcgt tcaatccagc
     7141 gtgtaccaat tttcatcacc accaggccaa tgacggtata aaccagccca caggcgataa
     7201 tcccgcccag cgcgatgctg atattcgggt taatgccctg accgttaaag cccgtcgcgg
     7261 caatcaccac gccgacaaaa gccgcgctgg agccgagata actggggacg cgcccgccgg
     7321 taataaagaa aaacagtaac gtgccgatcc ccgacattaa aatggaaaga ttgggatcca
     7381 gccccatcag aatcggcatt aacaccgtcg cgccaaacat cgccaccgcg tgttgaacgc
     7441 ccattactgc cgtctgagca aacggcaatc gttcatccgg cgcgaccacg ccgctctctg
     7501 tagaggtcga ttttaactgc cagtgaggaa aaccgaacat tgccatcagc tgtctcctta
     7561 aggaggttaa caagcagggc gcatcagcgc gtgataactg cgatcgaacc acaccagccc
     7621 gtagggtgtg gtgtgacgat gaatcgcttc gatggcgcaa aacagaatgt cgtgggtgcc
     7681 gacgctcacc acctggctga tacggcagtc aaacgaaacc agagcctctt ccagttgcgg
     7741 gcatccggtc acacccgtct gccagcgggc ggcggcaaag cggtgttcca tgggcgtttt
     7801 gccgccaaaa agatttgaaa gcggctcctg cccggcgctc agtgtattta cacacagcgt
     7861 tcgattttca ttgaataccg gccagacgga cgccccacga ttcaggcaca ccagtaatgt
     7921 gggcggcgtg tcggtcacgc tgcagacggc gctggcggtg aacccggcgc gcccggctgg
     7981 accgtccgtg gtgatgatat tgaccgccgc gcccatacag gacatcgcat cgcgaaaggt
     8041 ttgttgatca actatgttca tagtttgctc cttacaacag cccgcaggct tcttcaaagg
     8101 acagacgtgg caggcgcgca aaaagtttgc tgctatcgcc atagccgata ttaatcagca
     8161 gattgctctt cagcgtgctg cccgcaaaaa aggcgtcgtc aacgtgttga cggtcaaaac
     8221 ccgacatcgg gccggtatcc agtcccagcg cacggcaagc gacgatcaga taagccgcct
     8281 gcatggaact gttgcgaaac gctgtttctt cggcaagttg tgggctggag gtaaaccaac
     8341 tgcgggcatc accgtgggga aacagtagtg gtaaccgttc ataaaattca ctgtcccagg
     8401 cgacgatagc ggtgacgggc gcggtcaggg ttttttgcag attgccgctg gaaagcgccg
     8461 ggcgcagacg ttcttttcct tctgcagtgc ggataaacac gatccgtgcc ggagaacagt
     8521 tagctgatgt cggcccccat ttcatcaggg cataaagctc ctgtaacgtc tcatcgctga
     8581 cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt gaacagggtg ctaagcgcac
     8641 ctgggctaac ggcttcgttc atagcaattc cttacagggc ggcttcacgg tgatgtaaca
     8701 ggctggcaag cccgttgagt aacagagcat taaacgtttc gggatcggtc acgttgcagg
     8761 cgtgtccgcc atagggcatc accattttct ggctatcggg cagggcggca tgaagttcac
     8821 tggaacatgc tgttggcacc agcagatcat cactggcgca gatgatttgc accgggcagc
     8881 ggatgcgatc cgcatggtga ctaaagtcag cgcgtttgag ggcgttaagt cgacgtagta
     8941 aattattttt gccctgaaaa tgcgccagtg ccagcgcgtc ttctgcctcc aggcgaggtg
     9001 cgcgggccgc catccagtcg gcgggataga ggaacaacgg ctgcgcttcc acccatgctt
     9061 gcgcgccgcc gctatacagt aatcgttcgc gaacctgaaa acagcggcgc gtatgggcgt
     9121 ttattcgtag ccagccgtta acgctgacca gcatagttac cgacgcggga taatccagcg
     9181 ccagctgcat ccccaccagc gcaccgagcg catggccgac caccgcgtaa cgctcaatcc
     9241 ctgcggctac cagcgcctga tgcagttccg ctgccatctg ggcgatactg taatcttctg
     9301 ccagtgtgtc gggattattg ccggtgccgc gctggtcgta acagactacc tgatactcct
     9361 gctccagcac cgccagttgc ggtaaccagt aactgccgct accgccaaga cccgaaatca
     9421 acaccactac gggtgcatca gcataagggg gagatgagag tgaaagtttc atcgcggcct
     9481 cacttggcga tatgcgcaat tgtggcgatt tccaccagcg cgtcaggttt taccagtccg
     9541 cactgaatgt agaatcgcgc cggtttatca cccggaaaaa actcggcgta gatttcgtta
     9601 atcgcggcgt aatttttcca gtcggtaata aagatgctgt tgaaggtcac atccgccatc
     9661 gtgccacccg ccgtctcgat caccttgcgg atagtttcca gaacgtggcg ggtttgcgcc
     9721 tttgggtcat cggcaaacag cacgttatta tgttgatcaa aagccagcgt accggagaca
     9781 tacaccacgc catcagccag cgtgccggga acgaaggggg ccagcggtgc gctgctgcca
     9841 gcgggaataa ttacggattt tggcatcgct aaactcctta agcgatacga gcaaaggacg
     9901 tgggagaaag cgcgtcgcag aacgtttcga cgtcgctgac ccagccaaaa aaggtttcga
     9961 tattgaacaa cgcggctttc tgtgcaaatt ccggccccgc ctggtgtgtt gcgtcttcca
    10021 gcaccacgcc gaaatgctcc agaaaaaagc catcgcgtag cgtcgattcg acgcagacgt
    10081 tggtagcgat accggtgaaa accagatggc gtattccacg gctgcgtaaa atgctgtcca
    10141 gcggcgtatt gaagaaaccg ctgtagcgcg gcttcggcag cacaatatcg ccaggctgcg
    10201 gcaccagttc atccaccagt tgataatccc aggagccttt cgccagcaat ttcccctgca
    10261 gctgcggctg cttacgcatg gttttcaggg cgttcgattt atgaaaattc ggtgagccgg
    10321 ggccgccagc ctcgacatac tgttcatccc agccattttg aaaccagatg atcaacatcc
    10381 ctgccgctcg cgctgcggtc acagcggttt gaatgttggc aatgaccggg cgagtggttg
    10441 agacatcaaa cccggcgaga tctaagtagc cgcctggcgt ggcataagcg ttttgcatat
    10501 ccaccacgat ctgcgcactt tgctgcggat cgaaggtaat ggcttccggt cgagcggtta
    10561 aggttgtcat catgccacct cctgagtcag cgcagagaga tgggcgcggc actgcatcag
    10621 tggttgaatg cgctcgccga aggtttcgat tcccgacaga aaatcatcga aggtcaacag
    10681 cacgccttct gcaccaggca cgcttgcgac ttcatctaac atgcgcgcga cactggcgta
    10741 agaaccgact aacgtcccca tattgatgtt taccgccgaa gtgggatcgg ccatctgacg
    10801 aacgttggtg tcagtaccgg agcgggtatc tttctgactt tgttcggtta gccagcttaa
    10861 cgcctcttca tccgcgcccg ctttgtagtg ttcccatttg gcgcgagtgg catcgtcggt
    10921 ttcatcggca atcaccataa acaacacata agagccaacg tcgcgtccgg tttgctctgt
    10981 ggcctgtttc atccgcgcag cggtcggggc gaaagccgtg ggtgtattta cgcctttgcc
    11041 gaaacagaag ttgaaatcgg catactgggc ggagaacgcc atgccagcgt cgctttgccc
    11101 ggcgcagatc actttcatgg ggatactcgg ttgcggactg acgcgacaat cattcatggt
    11161 gaaaaaatcg cctttaaaat cgcttttccc cgtgccccac aggtcgcgca gcacctgaac
    11221 atattcggtg agatagtcgt aacgacggga gaaatagtca tcgccaggcc agatacccat
    11281 ctgctcatac tcgggctttt gccagccagt cacgaggttg acgccaaaac gcccgccaga
    11341 gatggagtcg atggttgcgg ccatacgggc gacgattgcc ggaggtaacg ttaaggtggc
    11401 agcagtggcg taaatctgaa tgcgcgaggt cacggctgcc agacccgcca tcaaggtgaa
    11461 cgactcaagg ttgtgatccc agaactcagt tttgccgcca aagccacgca gtttgatcat
    11521 cgacagggcg aaatcgaaat ggtaatgctc cgctttttgc gcaatggctt tattcagttc
    11581 aaaggtcggc atgtactgcg gcgcgtgggt cgaaatgagc cagccgttgt tgccaatagg
    11641 tacgaatacg ccaattttca tcatcaacct ctcttcgtct cgtaaagtga aagtcagacg
    11701 gggcgctgca tcctgcatat ccttttcagc cgcgtattgg cttgttcgca aagcggatgc
    11761 cagtttttaa aaagtgaata ttattaatgt gttagcattg tgttatctaa aatatctggt
    11821 aaaaagtgga ctaaacggtc aaaacagttg cacataaaac atgcatccgt gcgcgatgag
    11881 agtgcagaag gtcgaggccg ggcgggggtt ttgctatcct gttaccaatc tacaagaggg
    11941 gagagcgcat gacgcaaggc gcagtgaaaa caacgggtaa acgttcgcgc gcagtaagcg
    12001 cgaagaaaaa agcgattctt agcgcagcac tggacacttt ttcacaattc ggttttcacg
    12061 gcacaaggct ggagcagatc gcagagttgg cgggtgtttc aaaaaccaat ctgctgtatt
    12121 acttcccgtc aaaagaggcg ctgtatattg ccgtgctgcg gcagattctc gatatctggc
    12181 tggcaccgtt aaaagcgttt cgtgaagatt tcgccccgct ggcggcgata aaagagtaca
    12241 tccgtctgaa gctggaagtc tcacgcgatt atccgcaggc ttcgcgcctg ttctgtatgg
    12301 agatgctggc aggcgcgccg ttgttaatgg atgaactgac gggcgatttg aaggcattaa
    12361 ttgatgagaa atcggcgctg attgccggtt gggtcaaaag cggcaaactc gcgccgattg
    12421 atccgcagca tttgattttt atgatttggg cttccactca acattacgcc gatttcgccc
    12481 ctcaggtgga ggcggtgaca ggcgcgacgt tgcgcgatga ggtatttttc aatcaaacgg
    12541 ttgaaaacgt gcagcggatt attattgagg ggattcgacc acgttaaaga tgacggagga
    12601 ggttgtaaca tcctccggct acctgtttaa ccgatcgtca ttaaactggc gttaccgcca
    12661 gcggcagcgg tattcacact cagcgaacgt tcatcgatag gaattaaaac cccaaaaaga
    12721 ttaaaaaaac accacaaaac ggat
//
LOCUS       DAJVQR010000102        12710 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVQR010000102 DAJVQR010000000
VERSION     DAJVQR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(221..940)
                     /locus_tag="OQM52_003258"
                     /pseudo
     CDS             complement(221..940)
                     /locus_tag="OQM52_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(956..1288)
                     /gene="cusF"
                     /locus_tag="OQM52_003259"
     CDS             complement(956..1288)
                     /gene="cusF"
                     /locus_tag="OQM52_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCS1564991.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1312..2694)
                     /gene="cusC"
                     /locus_tag="OQM52_003260"
     CDS             complement(1312..2694)
                     /gene="cusC"
                     /locus_tag="OQM52_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCS1564992.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2851..3534
                     /gene="cusR"
                     /locus_tag="OQM52_003261"
     CDS             2851..3534
                     /gene="cusR"
                     /locus_tag="OQM52_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS1564993.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3524..4966
                     /gene="cusS"
                     /locus_tag="OQM52_003262"
     CDS             3524..4966
                     /gene="cusS"
                     /locus_tag="OQM52_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS1564994.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5116..7353
                     /gene="nrfB"
                     /locus_tag="OQM52_003263"
     CDS             5116..7353
                     /gene="nrfB"
                     /locus_tag="OQM52_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCS1564995.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7340..10308
                     /gene="nfrA"
                     /locus_tag="OQM52_003264"
                     /pseudo
     CDS             7340..10308
                     /gene="nfrA"
                     /locus_tag="OQM52_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10309..11199
                     /locus_tag="OQM52_003265"
     CDS             10309..11199
                     /locus_tag="OQM52_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS1564996.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11382..12143
                     /gene="envY"
                     /locus_tag="OQM52_003266"
     CDS             11382..12143
                     /gene="envY"
                     /locus_tag="OQM52_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS1564997.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12189..12265)
                     /locus_tag="OQM52_003267"
     tRNA            complement(12189..12265)
                     /locus_tag="OQM52_003267"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12229..12231),aa:Arg,seq:tct)
     gene            12508..>12710
                     /locus_tag="OQM52_003268"
     CDS             12508..>12710
                     /locus_tag="OQM52_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1564998.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3109 a         3302 c         3226 g         3073 t
ORIGIN      
        1 gtcgcttata gccataaata atatttggta cgctcagaaa taatggtttg gtacacatct
       61 ggtacacaat atcacatgga caacagcgca cagtaaacaa ctatatttga cgttcgtaga
      121 catactcagg tgaaaaaaag gttgtttttc gagttataac agacagttaa ttaactacag
      181 tggacaatgc tagacaatga cagacacaga taagcaacct accttcctct ttcgcgacca
      241 cgttatcttt ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa
      301 tgggcgcttt gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg tggcgaatat
      361 ccgcctccgg cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg
      421 taccgccggt ttcgcgcaac agtaaatact cactctgcgc ttccacccag tcgggaatgg
      481 tcaggtcgag aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct
      541 tgtcgataaa cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga
      601 cattcgtcgg gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta gccgttttca
      661 cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat
      721 cggcatattt cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc
      781 gcgtattggg atacatcggg tcgtaccaga ataagatttt acgttctgcg gtcggcattt
      841 tttctgcgtg cggttccgcc tggtcaaacc aggtaaaacc tgccgctgaa ataataccgc
      901 ccgcgatcat gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact
      961 ggctgacttt aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca
     1021 ctttgtcgcc ggttttaatt tcactcattt tcgtctgcgg ggtgatggta aagcgcatgg
     1081 tcatctccgg ccagttgacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt
     1141 ccagatcaac accttttacc acaccggtgg cactaatgac ctgtggttgt gcttcgctca
     1201 tggtttcatg atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga
     1261 acattgcgac ttgcagtgct tttttcattt attctctcct ggagttaaaa attattgctg
     1321 ccaaccgccc cctaaggcgg tatacaaata aatttcgtta acctgacggg cataattcag
     1381 gtcaagtaaa gtttgtcggg ttgcaaataa agaacgctcg gcatccagca cttccagata
     1441 acttaccgcg ccgtgctgat ataatgcccg cgcccgttgc agggtaattt gcagcgacgc
     1501 cagataacgc tgctgggcgc tgatttgatc gtccaggctt tgacgtaatg ccagcgcatc
     1561 tgccacctct ttaaaggcgt tctggatttt ctgttcataa ttcaccaccg actgctgctg
     1621 gcgaatttcg gcgatatcca gattggcctg gttgcgtccg gcattaaaaa tggggatctc
     1681 aattttagga ataaaattcc acatcccgct gctagcgtta aataatgacg acagatcgct
     1741 gctggcggtt gatattccgc tggtcaggct gatagacggg aaaaatgccg cacgcgcagc
     1801 accaatatta gcattagccg ccattaacgc gtgttcagct tccataatat ccgggcgctg
     1861 caataagatt tgcgacgaca agcccgccgg taatttaacg ctttgcaggc tgccgctgtt
     1921 tactgtctgc gcttgcggta gcttgccgta gcttcccaat aacagttgca atgcattatt
     1981 cgcctgcgcc agttccccct gacgtttagc gatgtcactg cgggtacttt ctatcacccc
     2041 gcgagcctgt tccagcgcca gaacattgct gctaccggtc aacagttgtt tttcgacaaa
     2101 cgcatatgac tgctgataat tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc
     2161 cagctgctga ttgaaatagc tttgcgcgac attagaaacc agcaaaatat gcaccgcgcg
     2221 ctgggcttcc tcagtggcta aataattttg gcgctcggct tcgctcatgt tctttaagcg
     2281 accgaaaaaa tcgagatcaa agctggcgtt aaggccagtc gagaactccc gcgtcgtggc
     2341 tgaatcgcct ttaagattgc cgctccagct gccgctgccc tcgccattga gctgtgggta
     2401 gcggtcggca tcggtcagac gatattgcgc ccgtgcctcc tgcactttca gcgtcgccat
     2461 gcgcaaatcc cggttattca ccagcgcctc gctaatcagc gtcttcacct gattatcaac
     2521 aaaaaaggtg cgccagccca cgttctggta gttatccgct gcgttaacca ggccgttctg
     2581 gctgagtgag aactgctgcg gtacgggcat taccggacgc tgataatccg gtgccagtga
     2641 acaaccagtt agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag acataggctc
     2701 ataatttctg gtgattttat gtcgccaact ttactcgcca ggctctgatt ttccggtgac
     2761 aggaaaatga caaaattgtc attttgccaa taagcgattg ccatctgatc ccgctactct
     2821 agaattgccc gggcaacatg cggaggaaat atgaaactgt tgattgtcga agatgaaaag
     2881 aaaaccggag aatacttgac caaagggtta accgaagccg gttttgtggt cgatttggcc
     2941 gacaacgggc tgaatggcta ccatctggcg atgaccggtg attatgacct gataatcctc
     3001 gatattatgc tgccagacgt gaacggctgg gatatcgtgc gcatgttgcg ctccgccaat
     3061 aaagggatgc cgattctgtt gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg
     3121 ctggagttgg gggcagatga ctacctggtg aaaccgttcg cttttgctga actgctggcg
     3181 cgggtgcgca ctctcctgcg gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt
     3241 gccgatctga tggtcgatct cgtcagccgc aaagtcaccc gcagcggcac gcgcatcact
     3301 ctgaccagta aagaatttac tctgctggag ttttttctcc gccatcaggg cgaagtgctg
     3361 ccccgctcgc ttatcgcctc gcaggtatgg gacatgaatt tcgacagcga cactaacgcc
     3421 attgatgtgg cggtgaagct gctacgcggc aaaatcgaca acgactttga gccgaagctg
     3481 attcaaaccg tgcgcggcgt gggttacatg cttgaggtgc cggatggtca gtaagccatt
     3541 tcagcgcccg ttttcgctgg caacccgcct gacctttttt atcagccagg ccaccatcgc
     3601 ggcgtttttc gcctttgcat ggatcataat ccactcagta aaagtgcatt ttgccgagca
     3661 ggacattaat gatttaaaag agattagcgc cacccttgaa cgggtactaa atcaccctga
     3721 cgaaacgcaa gcccgacgct taatgacgct ggaagatatc gtcagtggtt attccaacgt
     3781 gttgatttcc ctggcagata gccacggcaa aacggtgtat cactcccccg gtgcgcctga
     3841 tatccgcgag tttgcacgtg atgccatacc cgataaagac gcgcggggtg gcgaggtgta
     3901 tctcctttcc ggcccgacga ttatgatgcc aggccacggt cacgggcata tggaacacag
     3961 caactggcgg atgattaact tgccggttgg cccgttggtg gacggcaaac cgatttatac
     4021 gctctacatc gcgctgtcga tcgattttca tcttcattac ataaatgatt tgatgaataa
     4081 acttattatg accgcatcgg taatcagcat cctgatcgtc tttatcgtgc tgttggcggt
     4141 acataaaggt cacgcgccga tccgcagcgt cagccgtcaa atccagaata tcacctcgaa
     4201 agatctcgac gttcgccttg acccgcagac cgtgccgatt gagctggaac agctggtact
     4261 gtcgttcaac catatgatcg agcgtattga ggatgtcttt actcgccagt ccaatttctc
     4321 agcggatatc gcccacgaaa ttcgcacacc aattactaat ctcattacgc aaacggaaat
     4381 cgccctcagc cagtcgcgca gccagaagga gctggaagat gtgctctact ctaatctcga
     4441 agagctgacg cgaatggcga aaatggtcag cgatatgctg tttctcgctc aggccgataa
     4501 caaccagcta atccccgaaa agaaaatgct caacctggcg gatgaagtcg gcaaagtgtt
     4561 cgattttttc gaggcgctag cggaagatcg cggcgtggag ttgcggtttg ttggcgacaa
     4621 acgtcaggtt gcgggcgatc cgttgatgct gcgtcgggcg ctaagcaacc tgctttctaa
     4681 tgcccttcgt tatacgccaa ccagagagac aattgtagta cgctgtcaga cggtcgatca
     4741 ccaggtgcaa gtttccgtcg aaaaccccgg tacgcccatt gcgcccgagc acttaccgcg
     4801 attgtttgac cgtttctatc gcgttgaccc ctcccgccag cgaaaaggtg aaggtagcgg
     4861 tattgggctg gcgatagtga aatcgattgt tgtcgcgcat aaaggcacgg ttgcggtaac
     4921 gtcagatgcg cgggggacaa ggtttgttat cacattaccc gcttaattat tcagcaaacg
     4981 gcaactttta taaccagtgt aaaaataacg tgccgcaata gtcatacata ttaatggtaa
     5041 aaagctgtca caattcataa aaaaccttaa tatacgccac cctaaacata accagcgtta
     5101 atgtaaggtt tttgtgtgga ctggcttctt gatgtttttg ctacctggct ctacggctta
     5161 aaagtaatcg cgataacgtt agcggtcatc atgttcatca gcgggctgga cgattttttt
     5221 attgatgtcg tctactgggt acgccgcatt aaacgcaagt tgagtgttta tcgccgctac
     5281 ccgcgaatga gttaccgcga actgtataaa ccagatgaaa aaccgttagc gattatggtt
     5341 ccggcgtgga atgaaacggg cgtcatcggc aatatggccg agctggcggc gaccacgctc
     5401 gactacgaaa actatcatat ctttgttggc acctacccca acgaccccga tactcaacgt
     5461 gatgttgacg aagtgtgcgc tcgctttccg aacgtgcata aggtagtctg cgcgcgtcct
     5521 ggtcccacca gcaaagccga ctgcctgaac aacgtgctgg acgccatcac ccagtttgaa
     5581 cgtagcgcca atttcgcttt tgctggtttt attctgcatg acgccgaaga tgtgatttca
     5641 ccgatggaat tgcgtctgtt caactatctg gtcgagcgta aagatctgat tcagatccca
     5701 gtgtatccgt ttgaacgcga atggacgcac ttcaccagca tgacttacat tgatgagttt
     5761 tcagaactgc atggcaaaga tgttccggtg cgtgaagccc tcgccggaca agtgcccagc
     5821 gcaggcgtcg gcacctgttt cagccgccgc gccgtgaccg cactgttagc tgacggtaac
     5881 ggtattgctt tcgacgtgca gagtcttact gaagattacg acattggctt ccgcctgaaa
     5941 gaaaaaggta tgacggaaat ttttgtccgt tttccggtgg tggacgaagc caaagaacgc
     6001 gagcagcgta aatttttaca gcacgcacgg acgtcaaaca tgatctgcgt gcgcgaatat
     6061 ttccccgata ccttttcgac tgcggttcga caaaaatctc gctggatcat cggcattgtt
     6121 ttccagggct ttaaaaccca taaatggacc tccagcctga cgctgaacta ctttctctgg
     6181 cgcgaccgca aaggggcaat cagtaacttt gtcagcttcc tcgcgatgct ggtgatgatc
     6241 cagcttttgc tgttgctggc gtatgaaagt ttgtggcccg atgcctggca tttcctttct
     6301 atttttagcg gcagcgcatg gttaatgacc ctgctgtggc taaactttgg cttgatggtt
     6361 aaccgcatcg tgcagcgagt gattttcgtc actggctact acggcctcac gcagggcctg
     6421 ctttccgtcc tgcgtctttt ctggggcaac ctgattaact ttatggctaa ctggcgcgtg
     6481 ctgaaacagg ttcttcagca cggcgatccg cgtcgcgtcg cgtgggataa aacaacgcat
     6541 gacttcccca gcgtcactgg cgatacccgc tcgttgcgcc cgttaggtca aattctgctg
     6601 gaaaatcagg tcatcactga agaacaactc gatacagcac tgcgtaatcg cgtcgaaggt
     6661 ctacgcctgg gcggttcaat gctgatgcag gggctgatta gcgccgagca gctggcacag
     6721 gcgctggcag agcaaaacgg cgtggcgtgg gaatccatcg atgcctggca gatcccgtcc
     6781 tcgctgattg ccgaaatgcc agcctccgta gcgctgcatt atgcagtact gccgctgcgt
     6841 ctggaaaatg atgagttaat tgtcggcagt gaagatggta ttgacccggt ttcgctggcg
     6901 gccctgacgc gtaaagtcgg acgcaaagtg cgttacgtca ttgttctgcg gggacaaatt
     6961 gtcacagggt tacgccactg gtatgcacgc cgacgcggtc acgatccgcg ggcaatgttg
     7021 tacaatgcgg ttcagcatca gtggctcacg gaacagcaga ccggtgaaat ctggcggcaa
     7081 tatgtgccgc atcagttcct gttcgccgaa atactgacca cgctcggtca tattaatcgt
     7141 tcagcaatta acgtgttgtt attgcgccat gaacgcagtt ctctgccgct cggcaagttt
     7201 ttggtcaccg aaggcgttat cagccaggaa acgttggatc gcgtcctgac aattcaacgc
     7261 gaattacaag tttcgatgca atcactatta ctcaaagcag gtttaaacac agaacaggtt
     7321 gcgcaactgg agtccgaaaa tgaaggagaa taaccttaat cgcgtcatcg gatggtctgg
     7381 tttactgctg acgtctttat tgagtaccag cgcactcgca gacaatatcg gcaccagcgc
     7441 agaagagctg gggctgagcg attatcgcca ttttgttatt tatccccgtc tcgataaggc
     7501 gctgaaggca cagaaaaata acgacgaagc aaccgccatc cgcgaatttg aatatataca
     7561 ccaacaggtg ccggataata ttccgctgac tttatacctt gcggaagcct atcgtcattt
     7621 tggtcatgat gaccgggcgc ggctgttgct tgaggatcaa ctgaaacgtc acccaggaga
     7681 tgcccgactt gagcgcagtc tggcggctat tccagttgaa gtgaaaagcg ttacgactgt
     7741 tgaagaactg cttgcccagc aaaaagcggg cgacgctgcg ccgaccctgc gttgtcgcag
     7801 tgaagtcggg cagaatgccc tgcggctggc acaattacct gtcgccagag cgcaactgaa
     7861 cgatgcgacg tttgctgcat cgccggaagg aaaaacgctg cgaaccgatc tgctgcaacg
     7921 ggcaatctac ctgaaacaat gatcccaggc agatacgcta tacaatgaag cacgccagca
     7981 gaacacatta agcgcggcag aacgccgtca gtggtttgac gtgcttcttg ccgggcagct
     8041 ggacgatcgg atcctggcac tgcaatcaca ggggatcttc accgatcctc agtcatatat
     8101 tacttacgcg accgcgctgg cttatcgtgg cgaaaaagca cgcctccagc attatctcat
     8161 tgaaaataag ccgctgttta ccacgggcgc acaagagaaa agttggctct atctgttatc
     8221 taaatacagc gccaaccccg ttcaggcatt ggcgaattat acggtgcagt ttgccgataa
     8281 ccgccagtat gttgttggcg cgacgctacc ggtgctgtta aaagaaggtc agtacgacgc
     8341 agcgcaaaaa ctgctcgcca ccctccccgc caatgaaatg cttgaggagc gttatgctgt
     8401 cagcgtggcg acccgtaaca aggctgaatc tctgcgtctg gcacgattgc tgtatcagca
     8461 agaaccgaca aatcttcccc gcctggatca actgacctgg caactgatgc agaacgagca
     8521 gtcacgcgaa gctgccgatt tattgctgca acgctatcct ttccagggcg atgcgcgtgt
     8581 cagccagact ttaatggcgc gactggcgtc tctgctggaa agtcatcctt acctagcaac
     8641 gccggcgaag gtggcgattt tatcgaaacc cttaccgctg gcggagcaac gtcagtggca
     8701 aagtcagttg ccgggtattg cagataattg cccggcaata gttcgcttgc tgggcgatat
     8761 gtcgccttcc tacgatgctg ccgcctggaa ccgtctggca aagtgttatc gggacacgct
     8821 acccggtgtg gcgttgtatg catggcttca ggccgaacaa cgacaaccga gcgcctggca
     8881 acatcgtgcg gtagcctatc aggcgtatca ggttgaggac tacgccaccg cactggcggc
     8941 ctggcagaaa atcagtcttc acgacatgag caatgatgat ctgctcgccg ccgccaacac
     9001 cgctcaggca gcgggaaatg gcgcagctcg cgatcgctgg cttcaacagg cagaacaacg
     9061 tgagctggga aacaatgctc aatactggtg gctgcatgcg cgacgttata tcccagctca
     9121 gccagaactc gcactgaacg atctcacgcg ctcaatcaat attgcccctt ctgccaactc
     9181 ttacgttgcg cgggcgacaa tttatcgcca acgtcataat gttccggccg cggtgagtga
     9241 tttgcgcgcc gcgctggaac tggaaccaaa taatagcaac acccaggcag cgcttggtta
     9301 cgccttgtgg gatagcggtg atatcgcaca gtcgcgggaa atgctcgaac aggcgcataa
     9361 agggcttccg gacgatccgg cactgatccg acaactggcc tacgtgaacc agcgtctgga
     9421 tgacatgcct gcgacgcagc actacgcccg gctcgtgatt gatgacattg ataatcaggc
     9481 ataaccccac tgaccccaga gcaaaatcag caacgcttca atttccgccg tttgcatgag
     9541 gaggtcggtc gccgctggac gttcagtttc gattcttcca tcggcttgcg ttccggcgca
     9601 atgagtaccg ctaacaataa tgtcggcggt gcagcgcctg ggaaaagcta tcgtagctac
     9661 ggacaactgg aagccgagta ccgcatcgga cgcaatatgc tgctggaagg cgacctgctc
     9721 tcagtttata gccgcgtctt tgccgatacc ggagaaaacg gggtgatgat gccggtgaaa
     9781 aatccgatgt ccggcaccgg tctgcgctgg aagccgctgc gcgatcagat ctttttcctc
     9841 gccgtcgaac agcagttgcc gctgaacggg caaaatggcg catccgatac catgctgcgc
     9901 gccagcgcct cattctttaa tggcggcaaa tacagcgacg aatggcaccc gaacggttca
     9961 ggctggtttg cccaaaacct gtacctcgat gcggcgcaat atatccgcca ggatattcag
    10021 gcgtggacgg cagattatcg cgtcagctgg catcagaagg tagctaacgg acagactatt
    10081 gagccttacg ctcacgttca ggacaacggc tatcgtgata aaggcactca gggcgcgcag
    10141 cttggcggtg tcggggtccg ctggaatatt tggaccggcg agacgcacta cgacgcctgg
    10201 ccgcacaaag tcagtctcgg cgtcgagtat caacatacct ttaaggcgat taatcaacgt
    10261 aacggagagc gcaacaacgc gtttctcacc attggagtgc actggtaaat gcgtaagttc
    10321 attttcgtat tgctgacact gcttttggtc agcccttttt cctttgcgat gaaaggtatt
    10381 atctggcaac cacaaaaccg agatagtcag gttaccgata cccagtggca ggggctgatg
    10441 agtcagttac gttttcaagg cttcgatacc ctggttttgc aatggacccg ttacggcgat
    10501 gcatttaccc agccagaaca gcgcacgtta ttgtttaagc gtgccgcagc tgcgcaacag
    10561 actggcctga agctgattgt cgggctgaac gccgatccgg aattttttat gcaccagaaa
    10621 cagtcgtctg cagcgctgga aagctatctt aatcgcctgc tggctgccga tctccagcaa
    10681 gccagattat ggagcgccgc gcctggcata acgccggatg gctggtacat cagcgcggaa
    10741 attgacgacc tgaactggcg cagcgaagcc gcccgtcaac ctttgctaac atggttaaac
    10801 aacgcgcagc ggctgattag cgatgtttca gcaaaaccgg tttatatcag tagttttttc
    10861 gccggaaaca tgtcgcccga tggctatcgc caactgctgg aacaagttaa agcaaccgga
    10921 gttaatgtct gggtacagga tggcagcggc gtggataaac ttaccgctga acagcgtgaa
    10981 cgttatctac aggccagcgc cgattgccaa agctccgccc cagcaagcgg cattgtttat
    11041 gaactgtttg tcgccggcaa aggcaaaacc tttacagcga aaccgaaacc ggacgcagaa
    11101 attgcctcgc tgttagcgaa acgttcctct tgcggtaaag acatgctcta tttctctctg
    11161 cgctatttgc ccgtcgcgca cggcattctc gagtattaaa tcccctccag gtaagtcggg
    11221 tacgacctgg cttacctctt tcgctctcgt cagaattcca tcaagattaa ttgctaaaaa
    11281 gcatgctaaa gaaagaagat cgttatcggc gaacgggccg atcactcatg caggaagcat
    11341 agatcctttg agcggagttt ctgatatctg aggagtgcga aatgcaattg agcagcagtg
    11401 aaccttgcgt ggtgatcctg accgaaaaag aggtagaggt aagcgtcaat aaccatgcta
    11461 cgtttaccct tccgaaaaac tacctggccg ccttcgcgtg caacaataac gtcattgaac
    11521 tctcaacgtt aaatcacgta ttaatcaccc acatcaaccg taacatcatc aacgattatc
    11581 tgttgttttt aaataagaaa ttaacctgtg taaagccctg gtcgcggcta gcaaccccgg
    11641 ttatcgcttg tcatagccgt acaccggaag tgttccggct agccaccaac cacagcaagc
    11701 agcaatccag caaaccctgc gaagcggagt tgacgcgcgc attacttttt accgtattgt
    11761 ctaactttct tgagcaatcg cggtttattg ccctactgat gtatatctta cgcagcagcg
    11821 tccgcgacag cgtctgccgc attattcaaa gcgatattca gcattactgg aatctgcgaa
    11881 ttgtcgccag ttcgctatgt ttaagcccca gcctgctcaa aaagaaatta aaaaacgaaa
    11941 ataccagcta tagccagatt gtcacagagt gtcgtatgcg ttacgccgta cagatgttat
    12001 tgatggataa caaaaatatc actcaggtgg cgcaattatg tggctatagc agcacgtcgt
    12061 actttatctc tgtttttaag gcgttttaca gcctgacacc gttgaattat ctcgccaaac
    12121 agcgacaaaa agtgatgtgg tgaagggcaa agcggaaacg gataagacgg gcataaatga
    12181 agaagaaatg gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg
    12241 ctctatccaa ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta
    12301 tacggtcaac ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc
    12361 gcgtctgatt ttctgattta tttcgctata gcggcaaaca aacgcacacc gctgcgcgtc
    12421 tgaatcaaga aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa
    12481 ttgtccgctc ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact
    12541 catcctatca tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt
    12601 gtcctgaaaa cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat
    12661 ttaatcatta tggatataga cttgcccgga acagacggtt ttaccttcct
//
LOCUS       DAJVQR010000103        12600 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVQR010000103 DAJVQR010000000
VERSION     DAJVQR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..1040
                     /locus_tag="OQM52_003269"
     CDS             <1..1040
                     /locus_tag="OQM52_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1564999.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OQM52_003270"
     CDS             1041..3206
                     /locus_tag="OQM52_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCS1565000.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3220..4131
                     /locus_tag="OQM52_003271"
     CDS             3220..4131
                     /locus_tag="OQM52_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCS1565001.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OQM52_003272"
     CDS             4131..5426
                     /locus_tag="OQM52_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCS1565002.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OQM52_003273"
     CDS             5471..5701
                     /locus_tag="OQM52_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565003.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OQM52_003274"
     CDS             5679..6179
                     /locus_tag="OQM52_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCS1565004.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OQM52_003275"
     CDS             6179..7597
                     /locus_tag="OQM52_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCS1565005.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OQM52_003276"
     CDS             7597..8445
                     /locus_tag="OQM52_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565006.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OQM52_003277"
     CDS             8445..8900
                     /locus_tag="OQM52_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCS1565007.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OQM52_003278"
     CDS             8903..9595
                     /locus_tag="OQM52_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCS1565008.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OQM52_003279"
     CDS             9605..10936
                     /locus_tag="OQM52_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCS1565009.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10937..>12600
                     /locus_tag="OQM52_003280"
     CDS             10937..>12600
                     /locus_tag="OQM52_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1565010.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLK"
BASE COUNT         3387 a         3121 c         3319 g         2773 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gatgcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 ttccaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac
    12181 agtagcagca caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc
    12241 tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca
    12301 cttttccggg aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc
    12361 tgctctacag gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat
    12421 aattgatcgc gtttccggtg caaaagatgc tcttggtatg agcgacaagt ttattgatac
    12481 ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac cagctttatc gcaatgttga
    12541 aaaggcgatg cctgcaggtg caaaaattga tgcgccatca acaaggtcaa tgctcaaaca
//
LOCUS       DAJVQR010000104        12530 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVQR010000104 DAJVQR010000000
VERSION     DAJVQR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            131..1828
                     /gene="maeA"
                     /locus_tag="OQM52_003281"
     CDS             131..1828
                     /gene="maeA"
                     /locus_tag="OQM52_003281"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1565011.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1962..2972
                     /gene="adhP"
                     /locus_tag="OQM52_003282"
     CDS             1962..2972
                     /gene="adhP"
                     /locus_tag="OQM52_003282"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCS1565012.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3118..3402
                     /locus_tag="OQM52_003283"
     CDS             3118..3402
                     /locus_tag="OQM52_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1565013.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3630..4283)
                     /gene="fdnI"
                     /locus_tag="OQM52_003284"
     CDS             complement(3630..4283)
                     /gene="fdnI"
                     /locus_tag="OQM52_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1565014.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4276..5160)
                     /gene="fdnH"
                     /locus_tag="OQM52_003285"
     CDS             complement(4276..5160)
                     /gene="fdnH"
                     /locus_tag="OQM52_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1565015.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5173..8220)
                     /gene="fdnG"
                     /locus_tag="OQM52_003286"
     CDS             complement(5173..8220)
                     /gene="fdnG"
                     /locus_tag="OQM52_003286"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7633..7635),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1565016.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8452..9333
                     /gene="yddG"
                     /locus_tag="OQM52_003287"
     CDS             8452..9333
                     /gene="yddG"
                     /locus_tag="OQM52_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1565017.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9588..10688
                     /gene="ompC"
                     /locus_tag="OQM52_003288"
     CDS             9588..10688
                     /gene="ompC"
                     /locus_tag="OQM52_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1565018.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVDYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10852..11337
                     /locus_tag="OQM52_003289"
     CDS             10852..11337
                     /locus_tag="OQM52_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1565019.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11447..12436
                     /locus_tag="OQM52_003290"
     CDS             11447..12436
                     /locus_tag="OQM52_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1565020.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3284 a         3105 c         2920 g         3221 t
ORIGIN      
        1 atgacgatta aacaccattg cctgcgcaat ggtgttttta tctgctttat acttggggac
       61 gacgccctgg cggtaaagca aagacgataa aagcgtgcca gggatggata ttcaaaaaag
      121 agtgagtgac atggaaccaa aaacaaaaaa acagcgttcg ctttatatcc cttacgctgg
      181 ccctgtactg ctggaatttc cgttgttgaa taaaggcagt gccttcagca tggaagaacg
      241 ccgtaacttc aacctgctgg ggttactgcc ggaagtggtc gaaaccatcg aagaacaagc
      301 ggaacgagca tggatccagt atcagggatt caaaaccgaa atcgacaaac acatctacct
      361 gcgtaacatc caggacacca acgaaaccct cttctaccgt ctggtaaaca atcatcttga
      421 tgagatgatg cctgttattt ataccccaac cgtcggcgca gcctgtgagc gtttttctga
      481 gatctaccgc cgttcacgcg gcgtgtttat ctcttaccag aaccgccaca atatggacga
      541 tattctgcaa aacgtgccga accataatat taaagtgatt gtggtgactg acggtgaacg
      601 tattctgggg cttggtgacc agggcatcgg cgggatgggc attccgatcg gtaaactgtc
      661 gctctatacc gcctgtggcg gcatcagccc ggcgtatacc cttccggtgg tgctggatgt
      721 cggaacgaac aaccaacagc tgcttaacga tccgctgtat atgggctggc gtaatccgcg
      781 tatcactgac gatgaatact atgaattcgt tgatgaattt atccaggctg tgaaacaacg
      841 ctggccggac gtgctgttgc agtttgaaga ctttgctcaa aaaaatgcga tgccgttact
      901 taaccgctat cgcaatgaaa tttgttcttt taacgatgac attcagggca ctgcggcggt
      961 aacagtcggc acactgatcg cagccagccg cgcggcaggt ggtcagttaa gcgagaaaaa
     1021 aatcgtcttc cttggcgcag gttcagcggg atgcggcatt gccgaaatga tcatcgccca
     1081 gacccagcgc gaaggattaa gcgaggaagc agcgcggcag aaagtcttta tggtcgatcg
     1141 ctttggcctg ctgacggaca agatgccgaa cctgctgcct ttccagacca aactggtgca
     1201 gaagcgcgaa aacctcagtg actgggatac cgacagcgat gtgttgtcac tgctggatgt
     1261 ggtgcgcaat gtaaaaccag atattctgat tggcgtctca ggacagaccg ggctgtttac
     1321 ggaagagatc atccgtgaga tgcataaaca ctgtccgcgt ccgatcgtga tgccgctgtc
     1381 taacccgacg tctcgcgtgg aagccacacc gcaggacatt atcgcctgga ccgaaggtaa
     1441 cgcgctggtc gccactggca gcccgtttaa tcctgtggta tggaaagata aaatctaccc
     1501 tatcgcccag tgtaacaacg cctttatttt cccgggcatc ggcctgggtg ttattgcttc
     1561 cggcgcgtca cgtatcaccg atgagatgct gatgtcggca agtgaaacgc ttgctcagta
     1621 ttcgccgctg gtcctgaacg gcgaaggtct ggtactaccg gaactgaaag atattcagaa
     1681 agtctcccgc gcaattgcgt ttgcggttgg caaaatggcg cagcagcaag gcgtggcggt
     1741 caaaacgtct gctgaagctt tgcaacaagc cattgacgat aatttctggc aagccgaata
     1801 ccgcgactac cgccgtacct ccatctaagc ctgcacccgg tagtgaaggc taccgggcta
     1861 tttccctctc cctttttcag atctcatcca tactgggtag tggcgaataa atctcatttg
     1921 cctcacctgc tatgcagaac atcatccgaa aaggaggaac tatgaagact gcagttgtta
     1981 cgaaggatca tcatgttgac gttacggata aaacactgcg ctcactgaaa catggcgaag
     2041 ccctgctgaa aatggagtgt tgtggtgtat gtcataccga tcttcatgtt aagaatggcg
     2101 attttggtga caaaaccggc gtaattctgg gccatgaagg catcggtgtg gtggcagaag
     2161 ttggcccagg tgtcacctca ttaaaaccag gcgatcgtgc cagcgtggcg tggttttacg
     2221 aaggatgcgg tcattgcgaa tactgtaaca gtggtaacga aacgctctgc cgttcagtta
     2281 aaaatgccgg atacagcgtt gatggcggga tggaggaaga gtgcatcgtg gtcgccgatt
     2341 acgcggtaaa agtgccagat ggtctggact cggcggcgcc cagcagcatt acctgtgcgg
     2401 gggtcaccac ctacaaagcc gttaaactgt caaaaattcg tcccggtcag tggattgcta
     2461 tctacggtct tggcggtctg ggtaacctcg ccctgcaata cgcaaagaat gtctttaacg
     2521 ccaaagtgat cgccattgat gtcaatgatg agcagttaaa actggcaacc gaaatggatg
     2581 cagatttagc gattaactca cgcaccgaag acgccgccaa aattgtgcag gagaaaaccg
     2641 gtggcgctca cgctgcggtg gtaacagcag tagctaaagc tgcgtttaac tcggcagttg
     2701 atgctgtccg tgcaggcggt cgtgttgtgg ctgtcggtct gccgccggag tctatgagcc
     2761 tggatatccc acgtcttgtg ctggatggca ttgaagtggt cggttcgctg gtcggcacgc
     2821 gccaggatct aaccgaagcc ttccagtttg ccgccgaagg caaagtggtg ccgaaagtcg
     2881 ccctgcgtcc gttagcggac atcaacacca tctttaccga gatggaagaa ggcaaaatcc
     2941 gtggccgtat ggtgattgat ttccgtcact aagaggtctt tgctgcgact cccatgttcg
     3001 ggtcgcagca tcgcacactc tccaacatga aatggctaaa tggattattg ttgcgtagga
     3061 aggaacaata atgatagtgt tcccagagaa gtaacgctac tgagagtaaa gacactaatg
     3121 aaaatggcca atcatccccg cccgggggac attattcagg aatcactgga cgaacttaat
     3181 gtcagcctgc gcgagtttgc cagagcaatg gaaattgcgc cctcaacggc aagccgattg
     3241 ctgaccggaa aagcagcttt gacgccagaa atggcaatta aactttccgt ggtgatcggc
     3301 agttcgccgc aaatgtggct gaatctgcaa aacgcctgga gtctggcaga ggcagaaaaa
     3361 acggtggttg tgtcgcgatt acgccgtctg gttacgcaat aaaaaatggc accctataaa
     3421 gcgccatttg aaactgatga caaacgcaac attgcctgat gcgctacgct tatcaggcct
     3481 acacgttcac ttcaatctat tgtatttgaa gggttttcat gccggataag gcattcacga
     3541 cgcatccggg atgaacaaag cccacgttgt ctacaatatg aaaatggcgc cttttacagc
     3601 gccattttca gcttaaagtt caaaagagat tatatccctt cttcactctc ttttttcgct
     3661 tctgccttct cgatttcacg ataccagcgc ggatggtgtt tcttcgccca gcgacggctc
     3721 accttccctt cgatcatccc tttaatcgac cctttcaccc agaatgccat atacatatgg
     3781 atcaggatgg cgtggatcag gatgataccc gcagccgcgt ggatcagcag gctgtagcga
     3841 acaacctgca tcgggaagta ctgcgcaaag tacggacgcc agataatcac cccggtcacc
     3901 agcagcacga aaatcatgct catgatcgac cagaacatca tcttttgccc ggcgttgtac
     3961 ttaccaacat ccgccacttt gtgctcattg ccttttaata cttcgacaat gttcaacagc
     4021 cacggaatat ctttcttatc cgggatgttg tgatgcacaa aacgcacaaa cataaacatc
     4081 agtgcgacga aaatcgcaat gccgaagaag gggtgcaaaa tgcgtcccat ctgcggcgta
     4141 ccgaaggttt gcgtcagcca ttgcagcgtc gggaagaaga acgaaatccc ggacagcgcc
     4201 accaggaaga agcaaatcac cacggtccag tgacaggcgc gatcaataaa tttggtgcgc
     4261 acaatcattt tcgacttact catgatgatc ctcctcgtca tcgtccactt ccttattcgg
     4321 gccaataccg atgtagtgga aaatcaaccc ggcaaaagtg gcaataaagc cagccgctgc
     4381 cagcggtttc aacgcgcctt tccacaggct taccgaggtg tcgatcttcg gatctttcgg
     4441 cagaccgtga tacagctccg gctgatcggc gtgatgcagc acgtacataa cgtgcgtacc
     4501 accgacccct tccgggttgt agacgccagc atgttcgtaa ccacgcgctt tcagtttcgc
     4561 cacgcgctgt tccgccagct ccagcatctc cttcttggtg ccgaagtgga tagccccggt
     4621 cggacaggtt ttcacacaag ccggttcctg gccgacgctg acgcgatcga cgcagagcgt
     4681 gcatttatat acccggttat cctctttgtt gaggcgcgga atattaaacg gacacccggc
     4741 aatgcagtaa ccacagccga tgcagttttc cgactggaaa tcgacaatcc cgttagcgta
     4801 ctgaatgatt gcaccagcag acgggcacgc cttcaggcag cccggatctt cacagtgcat
     4861 acagccgtct ttacggatca gccactccag cttgccattc tgttcggttt cgctaaagcg
     4921 catcaccgtc caggacttgg cgctcagatc ggcggggtta tcgtaaaccc cgacgcagtg
     4981 ccccacttca tcacggatgt cgttccactc cgaacacgcc acctgacagg ctttacagcc
     5041 gatacaggtg gaaacgtcga taagtttcgc gacttctgct ttgtaatcac gcacctgaga
     5101 aggcggcgtg atggagttag ttgcggacct tttgataata tcctgcgttt ccatagccat
     5161 ctgttcgccc ccttacgcct tctcgatgtt gactaagaac gctttatatt ccggcgtttg
     5221 cgagtttgca tcaccgacat tcggcgtcag agtgttagcg atataacctt tacgcgcgac
     5281 accctcaaag ccccagtgga ttggaatacc caccgtttca acctgctgac cgtttacatt
     5341 cagcggtttc agacggcgcg ttaccactgc caccgcgcgg ataaagcctc gcttgctgga
     5401 gacagtgaca cgatcgccat tattaatgcc tttcgccgcc gcctgcgttt cgctgatttc
     5461 cacaaactgt tccggctgag caattgcgtt gagcaacgcg tgcttggtcc aggtgtggaa
     5521 gtgctcggtc agacgatagg tcgtacccac atacgggaac tgctcttttt tacccatccg
     5581 cagcgcgtct tgttcataca gacgaacaac cgggttagac accacgttcg gatgcagcgg
     5641 gttagtgccc agcggcgttt caatcggctc gtagtgttcc gggaacggac cttccgccat
     5701 tttgttgatg gcaaacaggc gtcccatccc ttccggctgc ataataaacg gcccggttgg
     5761 cgtacccggt gcggcattgc cgaagtccgg aatatcgtta cccgtccact tgctgccgtt
     5821 ccactggatc agcatccgtt tcggatccca cggtttaccg ttgatatccg ccgaagcacg
     5881 gttgtagagt acgcgacggt tgagcggcca cgcccaggcc catcccagcg tattccccag
     5941 accggacggg tcggagttat cgcggtttgc catctggtta ccctgctctg tccagctacc
     6001 ggtgtagatc cagcaagaag atgcggttgt accgtcatca cgcagatgcg caaagctact
     6061 cagcaactga cctttcttcg caatcagcac gccattagca tcatagagat cttccaacgc
     6121 atagccgttg ttctctttag ctacttcgtc agattgcggt tcgtgcggct gcttgtagtt
     6181 ccagctcatc ttcatcagcg gttctacgcc tttaccacct tcggcctggt acaactcgcg
     6241 cagatgatgg tagatacccg ccagaatttc gccgtcgtta cgcgcttcgc ccggcgcgtc
     6301 ctgacctttc cagtgccact gcaaccagcg accggagtta gcgatagaac catcctcttc
     6361 agcaaagcag gtcgaaggca gacggaatac ttcagtctga atagacgccg gatcgacatc
     6421 gttcgactcg ccgtggttct gccagaaggt agaggtttca gtcaccagcg gatcgataac
     6481 caccatgtac ttcagtttgc tcaggcagct caccactttg tttttatccg ggaaggacgc
     6541 aaccgggtta aagccctggc agaaataacc ggtaactttg ccttcatcca tcatgttgaa
     6601 atacttgatg acgtcgtagg tctggtccca cttcggcagc cagtcatagc cccagttgtt
     6661 ctctttctgc gcggcatcgc catagaaaga tttcatcagg ctaacgaaga acttcggata
     6721 gttgctccag tagttcacct gatcagccag cgtcgctttc ggcgtgttcg cttccagata
     6781 cgactgcaaa tcaacctgtt tttctgacgg cagcgtcaga taacctggca ggctggtaga
     6841 gagcagacct aagtcagtca gcccctgaat gttggagtga ccacgcaatg cgttcacgcc
     6901 gccaccggcc atacccatgt taccgagcag caactggatc atcgccatag tacggatgtt
     6961 ctgcgcaccc acggtgtgct gcgtccagcc cagcgcgtac aggaaggtgg ttgtgcgatc
     7021 cggtgcgctg gtggaggcca gcacttcaca cactttcagg aagtcggctt ttggcgtacc
     7081 acagatgttt tcaacaacgt ccggcgtgta gcgggaaacg tgcgctttca gcaggttcca
     7141 cacacagcgc ggatgagtca gtgtttcatc gcgtttcgca tagccgtttt catcgaactg
     7201 atagttccag gacgatttgt cgtactggcg tttttcagcg tcataaccgc tgaacaggcc
     7261 atcttcgaaa gcaaaatcat cacgcaccag taggctggcg ttggtgtaat gtttaacgta
     7321 ttcggcgttg attttgttgt tttcgatcag gtagcgcaaa atgccagaca ggaacgtaat
     7381 gtccgtaccg gaacgaatag gtgcgtaaat atccgccaca gaagcggtac gcgtaaaacg
     7441 gggatcgaca acgatcaagg ttgcatcgtt gttgtttttc gcttccatcg cccagcggaa
     7501 accgacggga tgcgcttcag cagcgttacc gcccattacc attacgacgt tagcgttttt
     7561 gatatccacc cagtggttgg tcatagcacc gcgaccaaat gttggagcaa gacttgctac
     7621 cgttggtccg tgtcagacgc gcgcctggtt gtctaccgcc agcatcccga gggagcgggc
     7681 aaatttctgg gtcagcatcc cggtttcgtt gctggcaccg gaggcacaca gcataccggt
     7741 agaaagccaa cggtttaccg ttacgccctg ctcgttcttt tcaataaagt tagcgtcacg
     7801 gtcagctttc atcagcttcg caatacggga gaatgcttct tcccagctaa tgcgctgcca
     7861 tttgtcagaa cctggcgcac gatatttcgg gtagcgcaga cggttttcac tgttgacgta
     7921 atccagcaaa ccggcccctt tcgggcacag cgcaccacgg cttaccggat gatccgggtc
     7981 accttcaatg tgataaatcg cttctctggc gttttttgcg ccatcaccca ggctatacat
     8041 caatagcccg caacctacgg aacagtatgt gcaggtgtta cggatctctt tagcgcgtaa
     8101 taatttgtag tttcgcgcct gagccagtgc ttgcttcggg gcaaagccca atgccgctac
     8161 cgttgttcca gccataccgc ccgcgcagat tttaaaaaat tgtctgcgac tgacgtccat
     8221 tgctttcctc ttttttcagg ggtattactg cgcgaggaaa ctaacagtaa aattctgaat
     8281 ctttttgcgc gaaatcaaaa agaacacgtg tttgccacaa aataatccgc ctgcgcaaat
     8341 tgacctacct caatagcggt agaaaaacgc accactgcct gacaggccag ttaaaaaaat
     8401 gctataaaat tcagcttaat ttttaacggc aagagagaca aaacagcgag catgacacga
     8461 caaaaagcaa cgctcatagg gctaatagcg atcgtcctgt ggagcacgat ggtaggattg
     8521 attcgcggtg tcagcgaggg gctcggcccg gtcggcggcg cagctgctat ctattcatta
     8581 agcgggctgc tgttaatctt cacggttgga tttccgcgta ttcggcagat tccgaaaggc
     8641 tatttactcg ccgggagtct gttattcgtc agctatgaaa tctatctggc gctttcctta
     8701 gggtatgcgg cgacccgtca tcaggcgatt gaagtgggta tggtgaacta tctgtggccc
     8761 agcctgacaa ttctctttgc cattctgttt aatggtcaga aaaccaactg gctgattgta
     8821 ccaggattat tattagccct ggtcggcgtc tgctgggtgt taggcggtga gaatgggtta
     8881 cattatgatg aaatcatcaa taatatcacc accagcccat tgagttattt cctggcgttt
     8941 attggtgcgt ttatctgggc agcctattgc acagtaacga ataaatacgc acgcggattt
     9001 aacggaatta ccgtttttgt cctgctaacg ggagcatgtc tgtggattta ctattttctc
     9061 acgccacaac cagaaatggt atttagcacg cccgtcatga ttaaactcat ctctgcggca
     9121 tttaccttag gatttgctta tactgcatgg aatgtcggta tattgcatgg caatgtcacc
     9181 ataatggcgg taggctcgta ttttacgcct gtactttcct cagcgcttgc agccgtgctg
     9241 ctcagcgccc cgctgtcgtt ctcgttctgg caaggcgcac taatggtctg cggcggttcc
     9301 ctgctctgct ggctggcgac acgtcgtggt taaaataatt gccgggtcta cggcccggca
     9361 gttaatcaga taaattccga caatattaaa aaataaattt caaataccag taattcacct
     9421 tatatctcat ttcgacttcg atcacatatc gataaataat attattctat ttttctcatt
     9481 gtttttaatc accattctat tttaaaaaca gtatgtcatt tataaatgac gccgtttaaa
     9541 attcgtttag aaaatacgtt taacgataat taaaaggatt ctgttaaatg aaattaaaaa
     9601 tagttgcggt ggttgtaact ggtttgttag ctgcgaacgt agcacacgct gccgaagtct
     9661 ataacaagga tggtaataaa ctcgaccttt atggcaaggt taccgctcta cgttatttta
     9721 ctgatgataa gcgtgacgat ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa
     9781 cgcaaatcaa tgatcaaatg attggttttg gtcactggga atatgatttt aaaggctata
     9841 acgatgaagc caacggctcg cgcggcaaca agacccgtct ggcctatgct ggtttaaaaa
     9901 ttagtgaatt tggctctctg gactatggtc gtaactacgg tgtcgactac gacattggtt
     9961 catggaccga tatgttgcca gaatttggtg gcgatacctg gagtcagaaa gatgtcttca
    10021 tgacataccg taccaccggt ctagcaacct atcgcaacta cgatttcttt ggcttaattg
    10081 aagggctgaa ctttgccgcg caatatcaag gcaaaaataa acgtactgac aacagtcatc
    10141 tttatggtgc tgacgacacg cgtgccaacg gtgacggttt cggtatctcc tcaacttatg
    10201 tttatgatgg ctttggtatc ggtgcggtat ataccaaatc cgatcggaca aatgcgcagg
    10261 aaagagccgc agctaatcct ctcaatgcct ccggtaagaa tgcagaacta tgggccacag
    10321 gtataaagta tgatgccaac aacatctact ttgcagctaa ttacgatgaa acattaaaca
    10381 tgaccaccta tggggatggt tatatctcta acaaagcaca aagttttgaa gtggtggcgc
    10441 aatatcaatt cgacttcggc ttgcgcccat cactcgctta cctgaaatcg aaaggcagag
    10501 atctgggccg ctacgccgac caggacatga ttgagtatat cgacgttggt gcgacgtatt
    10561 tcttcaacaa aaatatgtcg acctatgttg attataaaat caacctgatt gatgaaagcg
    10621 actttacccg tgccgtagat attcgcaccg ataacatcgt cgctacgggc attacctatc
    10681 agttctaacg tcctctcctg accagccctt ctctgggctg gtttttgatt taagtgctgc
    10741 tgtatgactc cttaagtaaa aatgagcaat tcttgatatt tctggtcatt cacctcaatt
    10801 aatattattc catatcctcc ggggacatct tactataata tttattgatg agtgattatg
    10861 accaatatta atacagcttg tgtaaaaaat aatgccagtt atcaacttaa taacgcatta
    10921 cctaacaaag aaaccatctc cagcaatttt tgtgaacgac tagaacaatg gggtaataaa
    10981 tcggttaata atggtgaaga aagagcaatt gccgtggagc gaattaaaga agcttacaat
    11041 tcgaacatgg catctcttga tttgtcatat cttgatttga gtgaattacc gcctatcccc
    11101 tcgacagtta atacgttaaa tctggaaaac aattgtctaa cttgtcttga cttagctgac
    11161 aatgccagtc tcgttaatat caacctcagc gttaataaaa ttaacacgat aacctttcca
    11221 aaagaaccaa aactggaaaa catttatatt aatcataaca atctggaaag tttagattta
    11281 aaaaatcagc attcattggt taatctggaa gcgcaaaata atagcctaaa aaaatgatat
    11341 ttctgatagt tataaactga aatttcttaa tcttgattat aacaagctag catcgctgga
    11401 tgtctcccgg caagaatctc tgattgagtt aagtgcccat cacaatatga tcactgacct
    11461 tatattacac aatcatccca ggatgaaaac aatcacttta aacgacaacc atattgcaca
    11521 tttaaacgcc aaaaacacta caaaactgga atatttaaac ttaagcaata acaatttact
    11581 gccaaccaat gacattgatc aactaatatc atcaaaacat ctttggcatg tattagttaa
    11641 cggcatcaac aatgatccac ttgcacaaat gcagtactgg actgcagtaa gaaatataat
    11701 tgatgacact aatgaagtga ccattgattt atcaggactt aatttaacca ctcaaccacc
    11761 agggctgcaa aacttcacct ctatcaatct tgataataac caactcacac attttgatgc
    11821 aaccaactac gatagactcg taaagctaag tctgaatagt aatgctcttg agtcaataaa
    11881 ttttcctcaa ggcagaaatg taagtattac acatatatct atgaataata atgctctcag
    11941 aaatattgat atagataggc tttcatcagt tacttatttt agtgcggcac ataatcaact
    12001 agagtttgtg caattagaat cttgcgaatg gctgcaatac ctgaatctca gccataatca
    12061 attaactgat attgttgcag gtaataaaaa tgaactctta ctgctggatc tatcccataa
    12121 taaactaaca agtttacaca atgtcttatt tcccaacttg aatacgttac ttattaacaa
    12181 caatttgctt tctgaaatta aaatattcta tagcaacttc cgcaatgttc agacattaaa
    12241 cgctgctaac aaccagttga aatatataaa tcttgatttc ctgacttatc ttccatctat
    12301 caaaagttta agactggaca ataataaaat aacccacatt gatactaata atacatccga
    12361 tattggaact ttattcccca taataaaaca gagcaaaaac ttaaattttt taaatgtttc
    12421 tgggaagaac aattgaccta ctatgcagct catgttattt aatttgtttt ccccagcact
    12481 taagcttaat actggcctgg caattctttc gcctggtgca tttgaagttc
//
LOCUS       DAJVQR010000105        12307 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVQR010000105 DAJVQR010000000
VERSION     DAJVQR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            748..831
                     /locus_tag="OQM52_003291"
     CDS             748..831
                     /locus_tag="OQM52_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1565021.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(885..2237)
                     /gene="gorA"
                     /locus_tag="OQM52_003292"
     CDS             complement(885..2237)
                     /gene="gorA"
                     /locus_tag="OQM52_003292"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1565022.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2309..3151)
                     /gene="rlmJ"
                     /locus_tag="OQM52_003293"
     CDS             complement(2309..3151)
                     /gene="rlmJ"
                     /locus_tag="OQM52_003293"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1565023.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3354..5396
                     /gene="prlC"
                     /locus_tag="OQM52_003294"
     CDS             3354..5396
                     /gene="prlC"
                     /locus_tag="OQM52_003294"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1565024.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5404..6156
                     /gene="rsmJ"
                     /locus_tag="OQM52_003295"
     CDS             5404..6156
                     /gene="rsmJ"
                     /locus_tag="OQM52_003295"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1565025.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6205..7674)
                     /gene="dtpB"
                     /locus_tag="OQM52_003296"
                     /pseudo
     CDS             complement(6205..7674)
                     /gene="dtpB"
                     /locus_tag="OQM52_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7992..8426)
                     /gene="uspA"
                     /locus_tag="OQM52_003297"
     CDS             complement(7992..8426)
                     /gene="uspA"
                     /locus_tag="OQM52_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1565026.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8820..9155
                     /gene="uspB"
                     /locus_tag="OQM52_003298"
     CDS             8820..9155
                     /gene="uspB"
                     /locus_tag="OQM52_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1565027.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9226..10725)
                     /gene="pitA"
                     /locus_tag="OQM52_003299"
     CDS             complement(9226..10725)
                     /gene="pitA"
                     /locus_tag="OQM52_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1565028.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10957..12156
                     /locus_tag="OQM52_003300"
     CDS             10957..12156
                     /locus_tag="OQM52_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1565029.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3002 a         3125 c         3304 g         2876 t
ORIGIN      
        1 gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga taagtcaaaa
       61 acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg aattttgagt
      121 tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact ttgcgtcggt
      181 tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc acatctaacg
      241 gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt cagctggaga
      301 agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat cgggtcgctg
      361 ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc ggttttttta
      421 ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc taacgcatca
      481 atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc taattacact
      541 gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat aaacgctttc
      601 aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg gagtgagatt
      661 gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa gcggatgcat
      721 tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt tcttggggcg
      781 ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa
      841 cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg catggtcacg
      901 aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt tttggttgcg
      961 cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa gccaatgccg
     1021 tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg gcacggctgg
     1081 cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga
     1141 tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc aatcggcgga
     1201 tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta
     1261 aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag ttccactgca
     1321 cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata tttatcgacg
     1381 acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat gttgtcattg
     1441 gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg accatcttcc
     1501 agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat ggcgttggta
     1561 tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg
     1621 aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta
     1681 atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc
     1741 aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc tggaatatcc
     1801 gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg
     1861 ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta
     1921 tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg
     1981 gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata atcagggccg
     2041 tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacgcag
     2101 ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca tttctggccg
     2161 tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa
     2221 tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt gtagcgcgag
     2281 acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt acggtggcgt
     2341 gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta ttcatctgct
     2401 gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg ccacggcgat
     2461 cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca
     2521 ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac aatgcgtaag
     2581 tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc tgatagtcgg
     2641 ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc ggcggcagtt
     2701 tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg tctttctgaa
     2761 atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc aggctgtctt
     2821 gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc aactggccgc
     2881 tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc aaatcgtcct
     2941 gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca tgttcgctac
     3001 ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct
     3061 ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc
     3121 cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatt
     3181 ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc
     3241 cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc tacacacatt
     3301 atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga
     3361 atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg
     3421 ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc gtagtagcgc
     3481 aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg gacgatgttt
     3541 tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaactgc
     3601 gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg gtagggcaac
     3661 atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac gccacgctga
     3721 acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc tctggcatag
     3781 gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt tctgaactgg
     3841 gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa ctcgttaccg
     3901 acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa gcccaggccg
     3961 aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac ctgccggtaa
     4021 tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac agcacccgcg
     4081 cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg gaagagatcc
     4141 tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc tttaaatccc
     4201 ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc gatctggcaa
     4261 aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc gctaaagccg
     4321 aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa aaacagaaac
     4381 agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa aacaaagcgg
     4441 ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgtaaag
     4501 atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa aataacgaac
     4561 tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc ggggcgtgga
     4621 tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa ccggtcgcgt
     4681 atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc actcacgacg
     4741 aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg acccgcatcg
     4801 aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc
     4861 agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct ggtcactatg
     4921 aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg aagaactacc
     4981 aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc cgccttcatg
     5041 ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa atcaagaaac
     5101 tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc agccatattt
     5161 tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta ctggcggcag
     5221 atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc
     5281 tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc aaacgcttcc
     5341 gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag ggctgatcat
     5401 tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg
     5461 gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacgccg
     5521 gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt
     5581 gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg
     5641 aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac cgcagggctg
     5701 gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct ggagcgtaat
     5761 ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc
     5821 ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac ggcgctaacc
     5881 gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca taagcagaaa
     5941 agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat
     6001 gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc
     6061 ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt gactaaaggg
     6121 catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt agcgataacg
     6181 ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca tgcgtttcag
     6241 ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac caatcttacc
     6301 aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc
     6361 ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat
     6421 cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg ccaggccaag
     6481 ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga taaaccatgg
     6541 cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg tcaaaaagcc
     6601 cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt ctttgccttt
     6661 gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg
     6721 gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat gatgcacgtt
     6781 gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac
     6841 cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt
     6901 gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca gcaccagatt
     6961 ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca cgctgccaag
     7021 taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag aaccaatgtc
     7081 tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc ccgccccgca
     7141 caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca gcgataacgc
     7201 tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat caagccgcgg
     7261 atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt
     7321 accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc
     7381 ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc gtttggtccc
     7441 caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca gtgcagcaaa
     7501 agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa cgaagaaaac
     7561 cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat
     7621 catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat taatatttat
     7681 taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt
     7741 tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac tattacaaca
     7801 gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa ttttgtattg
     7861 gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta cgctgcatca
     7921 ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg
     7981 tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc aacgtgaacg
     8041 gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca
     8101 caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg gcctaggtcg
     8161 ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag ctcggtcagc
     8221 gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac atcaataagc
     8281 ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtag
     8341 gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga gagatcgact
     8401 gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg
     8461 gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg
     8521 ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga
     8581 tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga atattatatt
     8641 gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg
     8701 ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag
     8761 cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta
     8821 tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc
     8881 gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct
     8941 atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc
     9001 gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc
     9061 gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt ggtctggtgg
     9121 tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa taacgtataa
     9181 aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca ggaactgcaa
     9241 ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa acacccaggc
     9301 catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc
     9361 cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat aactcgccag
     9421 gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt
     9481 cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat
     9541 acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga tggtgctaag
     9601 catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac caggcatctt
     9661 caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
     9721 atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt tcaggcggtt
     9781 gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg ttgcgccagc
     9841 ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg caggatgctg
     9901 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
     9961 ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca acataaccag
    10021 accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag
    10081 gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
    10141 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
    10201 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
    10261 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
    10321 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
    10381 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
    10441 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
    10501 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
    10561 aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
    10621 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
    10681 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
    10741 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
    10801 tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt atcttatata
    10861 attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt
    10921 acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga tgccattatt
    10981 ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt aggacgccgg
    11041 gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc tggcggtggg
    11101 cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca gaatccgcat
    11161 ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct ggtcaataaa
    11221 cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga ctccgcgcag
    11281 cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt cagattgcgt
    11341 agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct gaacggcatg
    11401 actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc ggggctgggc
    11461 gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct gccgacccgc
    11521 gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca ggtgctggcg
    11581 ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg tgagaactta
    11641 ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag ctactggcaa
    11701 ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac cttcctgaat
    11761 gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt tcatctaccg
    11821 aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc gctaaaacag
    11881 ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg cgtacaaccc
    11941 aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa
    12001 ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggtgaagtg
    12061 atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcatgg
    12121 gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc cgtcagccgt
    12181 ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt ctggtgttaa
    12241 ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat agtgtattta
    12301 ttctcag
//
LOCUS       DAJVQR010000106        12220 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVQR010000106 DAJVQR010000000
VERSION     DAJVQR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..493)
                     /locus_tag="OQM52_003301"
     CDS             complement(<1..493)
                     /locus_tag="OQM52_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1565030.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OQM52_003302"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OQM52_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1565031.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OQM52_003303"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OQM52_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1565032.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="OQM52_003304"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="OQM52_003304"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1565033.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="OQM52_003305"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="OQM52_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1565034.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="OQM52_003306"
     CDS             3647..4156
                     /locus_tag="OQM52_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1565035.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OQM52_003307"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OQM52_003307"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1565036.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="OQM52_003308"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="OQM52_003308"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1565037.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="OQM52_003309"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="OQM52_003309"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1565038.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="OQM52_003310"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="OQM52_003310"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1565039.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="OQM52_003311"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="OQM52_003311"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1565040.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="OQM52_003312"
     CDS             complement(8798..9691)
                     /locus_tag="OQM52_003312"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1565041.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="OQM52_003313"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="OQM52_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="OQM52_003314"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="OQM52_003314"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCS1565042.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="OQM52_003315"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="OQM52_003315"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCS1565043.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAJVQR010000107        12171 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVQR010000107 DAJVQR010000000
VERSION     DAJVQR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(113..489)
                     /gene="rnpB"
                     /locus_tag="OQM52_003316"
     ncRNA           complement(113..489)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQM52_003316"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(522..1667)
                     /gene="garK"
                     /locus_tag="OQM52_003317"
     CDS             complement(522..1667)
                     /gene="garK"
                     /locus_tag="OQM52_003317"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1565044.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1764..2654)
                     /gene="garR"
                     /locus_tag="OQM52_003318"
     CDS             complement(1764..2654)
                     /gene="garR"
                     /locus_tag="OQM52_003318"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1565045.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2684..3454)
                     /gene="garL"
                     /locus_tag="OQM52_003319"
                     /pseudo
     CDS             complement(2684..3454)
                     /gene="garL"
                     /locus_tag="OQM52_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3470..4804)
                     /gene="garP"
                     /locus_tag="OQM52_003320"
     CDS             complement(3470..4804)
                     /gene="garP"
                     /locus_tag="OQM52_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1565046.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5179..6750
                     /gene="garD"
                     /locus_tag="OQM52_003321"
     CDS             5179..6750
                     /gene="garD"
                     /locus_tag="OQM52_003321"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCS1565047.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6802..7626)
                     /gene="agaR"
                     /locus_tag="OQM52_003322"
     CDS             complement(6802..7626)
                     /gene="agaR"
                     /locus_tag="OQM52_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCS1565048.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7875..9155
                     /gene="kbaZ"
                     /locus_tag="OQM52_003323"
     CDS             7875..9155
                     /gene="kbaZ"
                     /locus_tag="OQM52_003323"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCS1565049.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9178..9651
                     /gene="agaV"
                     /locus_tag="OQM52_003324"
     CDS             9178..9651
                     /gene="agaV"
                     /locus_tag="OQM52_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCS1565050.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9662..10441
                     /locus_tag="OQM52_003325"
     CDS             9662..10441
                     /locus_tag="OQM52_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCS1565051.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10431..11240
                     /locus_tag="OQM52_003326"
     CDS             10431..11240
                     /locus_tag="OQM52_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCS1565052.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11327..11761
                     /locus_tag="OQM52_003327"
     CDS             11327..11761
                     /locus_tag="OQM52_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1565053.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11758..>12171
                     /locus_tag="OQM52_003328"
     CDS             11758..>12171
                     /locus_tag="OQM52_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1565054.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2906 a         3280 c         3102 g         2883 t
ORIGIN      
        1 ggtgaaactg accgataagc cgctttcttt tgggtatagc gtcgtggaca gtcattcatc
       61 tttctgcccc tccaaaagca aaaacccgcc gaagcgggtt tttacgtaaa acaggtgaaa
      121 ctgaccgata agccgggttc tgtcgtggac agtcattcat ctaggccagc aatcgctcac
      181 tggctcaagc agcctacccg ggttcagtac gggccgtacc ttatgaaccc ctatttggcc
      241 ttgctccggg tggagtttac cgtgccacgg actgttacca gccgcgcggt gcgctcttac
      301 cgcacccttt cacccttacc tgatcccgct tgcacgggcc atcggcggtt tgctctctgt
      361 tgcactggtc gtgggtttcc cccccaggcg ttacctggca ccctgcccta tggagcccgg
      421 actttcctcc cctccacccg tctcccccga agaggacgac gacgaagcgg cgactgtctg
      481 gtcagcttcg gcgcgcagta tagagggttt gcgcgccctt gtcaccccgt gttgcgcatt
      541 ccaatcgcca gtgtcgcggc aatattacgt gaagcacggc agatattgtc ataagccccg
      601 cggaatgctt cgtccaacgt acctatgctg gtcaatacgc tgaagaccgc atcaatgcca
      661 tgctgatgta caacgccaac atcatcggtc aggctacccg caatgccaat caccggttta
      721 tggtacttct tcgccacgtt tgcgacgcca atcggtacct tgccgtgaat actctggctg
      781 tcaatacgcc cttcaccggt gatcaccagc gtacaatcgt gaatatgttc ctccagattc
      841 agcgccgtag tgacgatttc aataccactt ttcagttccg caccaagaaa cgccatcagc
      901 gccgcaccca taccgcccgc agctcctgcg ccggggacat cttttacatc aacatgcagc
      961 gcttttttaa tgacatcggc atagtgagag aggttattgt ccagctcaac aatcatcgct
     1021 tcactggctc ccttttgtgg gccaaagatg cgcgatgcgc cgttatcgcc caccagcgga
     1081 ttggtgacat cacaagcgac gcgaatgacg caatctttta agcgcggatc gaggccggaa
     1141 atatcaatat cattcagagt attaagacta ccgccgccaa aaccaatttc attgccgttg
     1201 gcgtcgcata atttcgcccc cagcgcctgt accatgcctg cgccgccatc atttgtagcg
     1261 ctaccgccaa tgccgataat aatgtttgtc gcgccgctct ccagcgcctg caggattaac
     1321 tcgcctgtgc cgcgtgaagt ggtcacgagt ggatcgcgtt tttccgcagg taccagctcc
     1381 agcccactgg ccgccgccat ttcaataaac gcggttttgc catcgccgga gatcccccaa
     1441 ctggcattca ctttctcgcc cagcggccct gtaacccagg cgtgacgttc agaaccctgg
     1501 gtggctgcaa tcatcgcttc caccgttcct tcgccaccgt cggcaaccgg aatagaaacg
     1561 tactgtgcat caggaaaaat ttcccgaaat cctttttcta tcgcctgcgc aacctcgctg
     1621 gcagataaac tttctttata agagtctggg gcgattacga ttttcatacc tatgcctgtt
     1681 accacatgac gccggagggc gtttctctta ttcggcctgg attccaggcc cggattgcaa
     1741 tacgccatcc gggcacgacg tcattaacga gtaacttcga ctttcgccag tttttcgtag
     1801 tagcacacca gggcgctatg atccgccgtt cctaaaccat ctgctcgcag tgcctgcatc
     1861 atctccataa ccgcagctgt gagcggcagt tgcgcgccga cgccgtggga agtatccagc
     1921 gcatttgcca gatccttaat atgcagatca atacggaagc ccggcttgaa gttgcggtcc
     1981 atcaccatcg gcgctttggc atccagcacg gtactgcccg ccagtccacc gcgaattgcc
     2041 tgatacacca ggtccgggtt aacgcccgct ttagttgcca gcgttaacgc ttctgacatc
     2101 gcggcaatat tcagcgccac aatgacctga ttcgccagtt tggtgacgtt acctgcacca
     2161 attgccccgg tatgcaccac ggaacccgcc attgctttca tcaaatcata gtatttgtcg
     2221 aaaatagcct tgtcgccgcc caccatcact gacagcgtac cgtcgatggc tttcggttca
     2281 ccgccgctca ccggagcatc cagcatatca atgcctttcg ctttcagcgc ttcgctgatt
     2341 tcacggcttg ccagcggtgc gatagaactc atatcgatca ataccgtacc tggcttcgcg
     2401 ccttcaataa tgccattctc acccagcgcc acctctttca catgagggga gtttggcagc
     2461 atggttatga tgacgtcgca ctgttcagcg atcgctttag ccgtagaggc tgtttctgca
     2521 cctgcagcaa tcacgtcagc aatagcttct gggttacggt cagcaaccac cagcgagtaa
     2581 cctgctttca gaaggttttt actcattggt ttacccataa tccccaggcc aataaaacca
     2641 actttcatag tcatatcaat catctctctt gttgcggtgg tggttatttt ttaaaggtat
     2701 cagccagttt atgagtggca gagcggaaga cgccgagatc gctgccgaca gccacaaacg
     2761 tcgcgcccca ttccagataa cgacgcgcat cggcttcgac cggcgcgagg ataccgctgg
     2821 gtttgccgtg cgcgctggca cggttaaaaa tgtgctgaat tgctttttgt acatccgggt
     2881 gtgatgcatt gccgagatgg cctaatgccg cggccagatc gctggggccg acgaagatgc
     2941 cgtctacgcc ttcggtagcg gcaatggcat cgacgttatc tacgccctgc tgactttcta
     3001 tctggaccag aatagtgatg ttcttgttcg actgagcgaa ataatccgcc acggtgccaa
     3061 acatattggc gcggtgagaa acggagacgc cgcgaatgcc ttccggtggg taacgggttg
     3121 atgccaccgc ctgctctgct tcctcttttg tttctacaaa aggaatcagg aagttataga
     3181 aaccgatatc cagaagacgc ttaataattt ccggctcgtt ggtcggcact cacactactg
     3241 gcgcgctggc gctgcctttc aaggccatta actgcggaat aaacgtggag atatcgtttg
     3301 gcgcatgttc gccatccagc accagccagt caaacccagc caaaccaaga acttcagtgc
     3361 taatcgggtt agagagtgct gaccagcaac caatttgtac ctgtttcgca gccagtgcgg
     3421 ctttgaattt attcgggaaa acatcgttat tcatcgctta tacctttgct tatttctgca
     3481 attccatacg tttaatgtcg ccaactacga agaggtagca gaccatcgcc atcagcgctg
     3541 aacatcccac gaaaaccagt gctgcattga aggagtgcag ttcacttacc aggtagccaa
     3601 tcaccagtgg agtgacaatg gaggcaacat tgccaaagac gttaaagacg ccgccacaga
     3661 ggccaacaat ctctttcggc gcggtgtcag aaatcaccgg ccagcccaga gcaccaaatc
     3721 ctttgccaaa aaaagccagc gccatcagca tgaccaccag cgtggtgttg ttggtgtagt
     3781 tacataagat gatggtggaa gccagcaaca ttcccagcac aatcggtagc ttacgtgcca
     3841 gggtcaggga taaaccgcgt ttgatcagat aatccgagaa gacacctccc agcacgccgc
     3901 ccgcaaaacc acacagtgcc ggaatcgagg cgaccagacc cactttcaga atcgacatgc
     3961 ctttttcctg caccagataa atcgggaacc aggtgaggaa gaaccaggtg atggtgttga
     4021 taaaatattg tccgaaaaat acgcccaaca tcatgcggtt agagagcaat tgcttgatgt
     4081 aatgcagttt gggaccgctt gctgccgcgc tgcccggctt tttgtggtcc atatcgacca
     4141 ccgcgccatt ttcagagata aacttcagct cttcctcaga catacgtggg tgatctgtcg
     4201 ggttatgaat caacttgatc cacagcgccg tcagcacaaa acaaatcacc cccataacgg
     4261 taaagacgtg ctcccatccc caggcgaaag tcagccagcc aagcagaggc gaaaagagcg
     4321 ccagcgagaa atattgtgcc gagttaaaga tggcggaggc agtaccacgt tctttcgtcg
     4381 ggaaccaggc ggcgacaata cgggcgttcg ccgggaagga tggagcttcc gagaatccca
     4441 gcataaagcg cataaagaac atggagatcc ctgcccaagc cagcgggaac atatcaacaa
     4501 agccttgcag gaaggtgaac agcgaccaga aaaagaggct gtaggtgtaa acttttttcg
     4561 agccaaactt atcaagcaac cagccgccgg ggatttgcat cagcaagtag gcccagccaa
     4621 aagcggagaa gatgtaaccc atcgaaaccg cacttaactg caactctttt gccacttcgg
     4681 taccagcaat agacagcgtt gcacgatcgg cgtagttaac ggcggtaaca ataaaaataa
     4741 tcagtaatat taaatagcgg gtatgcacac ctttcctttt ttcgtcaacg gtgtccagaa
     4801 tcattttatt tacctcgggt acttatgctg atttttatta ttatggggaa ggtgttattt
     4861 ataagtttca tttatgccgt aacgacaatg aactcgggaa ttagtataag cagcgcgaga
     4921 ataataatca ttgtgcaaat gctaatttaa ttaatacttt ttaaatatta ttttgagcat
     4981 atgcacataa ggttgcgcgc taaagcacag atttgcgctt taccttgccg ggcggcactg
     5041 caatccctaa aatgattgac attgatcaca tttctgcgtt taaactcctg acattcttat
     5101 ttcacccaat gaaatcattt atttttaaat gagaccaggt cctcatttta ataacccctg
     5161 gctggagaat attgcacaat ggccaacatc gaaatcagac aagaaacgcc aactgcgttt
     5221 tatataaaag ttcacgacac agataatgtg gcaattattg ttaatgataa tggcctgaaa
     5281 gcaggaacgc gttttccgga tgggctggaa ttaattgaac atattcccca ggggcataaa
     5341 gtcgcattgc tggacattcc ggctaatggt gaaattattc gttatggcga agtgattggt
     5401 tacgccgtgc gtgcaatccc acgcggaagc tggatcgacg aatcaatggt tgtactaccg
     5461 gaagcgccgc cgttacacac gctgccactg gcaaccaaag tcccggaacc cttaccgccg
     5521 ctggaaggat acacctttga gggctatcgc aatgccgatg gcagcgtggg caccaaaaac
     5581 ctgctcggca tcaccaccag cgtccactgt gtggcaggcg tggtggacta tgtagtaaaa
     5641 atcattgaac gcgatctgct accgaaatac ccgaacgtcg atggcgtggt ggggctgaat
     5701 catttgtacg gttgtggcgt ggcgattaac gcaccggcag cagttgtacc tattcgtacc
     5761 attcacaata tttcgctgaa ccctaacttt ggcggcgaag taatggtgat tggcctgggt
     5821 tgtgaaaagt tgcagcctga gcgcctgctg actggaacgg atgatgtgca agctattcca
     5881 gtagaaagcg ccagcattgt cagtttgcag gatgaaaagc atgtcggttt tcagtccatg
     5941 gtcgaggata ttttgcaggt cgccgaacgc catctacaaa aactgaatca acgtcagcga
     6001 gaaacctgtc cggcttcaga actggttgtc ggcatgcatt gcggtggcag cgatgcgttt
     6061 tctggcgtaa cggcaaaccc ggcggttggc tatgcgtctg atctactggt gcgctgcggc
     6121 gcaacggtga tgttttcaga agtcacggaa gtgcgtgacg cgatccatct gctgacacca
     6181 cgcgcagtga acgaagaggt cggcaaacgg ctgctggagg agatggagtg gtacgataac
     6241 tatctcaata tgggaaaaac cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc
     6301 ggtctagcaa acgtggtaga gaaagcgctc ggctccattg ctaaatcggg caaaagtgca
     6361 attgttgaag tgctgtcgcc cggtcaacgc ccgactaaac gcggattaat ttacgccgcg
     6421 acgccagcca gcgattttgt ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa
     6481 gtgtttacga ccggtcgtgg tacgccgtac ggcctgatgg cggtacccgt cattaaaatg
     6541 gcaccccgca ccgagctggc gaaccgctgg tttgatttaa tggatattaa tgcgggcacc
     6601 atcgctaccg gcgaagaaac tattgaagag gagggctgga agttgttcca ctttattctc
     6661 gacgtcgcca gcgggaagaa gaaaaccttc tcggatcaat ggggactgca taaccagctg
     6721 gcggtgttta acccagcacc ggtgacctga taattcaatt cgtattatgc ccgatagcac
     6781 acgtttatcg ggcatattga gtcacagcga agacgcgtga tccccgacca gaatcacttc
     6841 aaccccagcc tttcgcagtc cttccagact atccgcagga atgccttcat caacaatgat
     6901 catgtcgata cgttgagtat caatgatctt atgtaaactg gaacgattga atttactgga
     6961 atcggtgacc acgatgatcc gttccgcaac ttcgcacatc cgacggctta aacgggcttc
     7021 atcttcatta tgtgtgctga cgccgcgctc cagatcgatc gcatctacac caagaaacag
     7081 catatcgaag tggtaatttt gcagcgattg ctcagcctga tcgccgtaaa aagattgcga
     7141 ctgacggcgc aaatgcccgc cggtcatcag cagctcaacg ccttccgctt ccagcaacgc
     7201 attagccacg ttcataccgt tggtcatcgc aattacgtca gtgtacttgc gcatcagacg
     7261 agcaatctca aaagtggtgg tcccggaatc gaggatcacc cgatgacctg gctgaatcaa
     7321 ctcaacggca gctttcgcaa cgctgcgttt catcgcggtg ttcagtgcgc ttttatcttc
     7381 cactgatggc tcgactgacg gcgtcgtgct atcgcagatc aacgcgccac cataggcacg
     7441 cacagcgatc ccctgctttt ccagaaacgc cagatcattg cggatcgtca cagtagatac
     7501 gccatacaat gccgacagat cgttaacctg cacactccct tgctgtcgca gacgctgaat
     7561 gatctgttct cgtcgctcgc tggtgcctgt cactcgcttc tcacctgaag cgtcggtatt
     7621 actcatagta agtcctttcg taaaactttc gtttcatttc gttttgccta ttaacgcctt
     7681 tctattaagc aaatgcaagc ccaccttgcc cattggcgca agctactctc gtttcagtga
     7741 ctttcattac gtttcttttg tgaatcagat cagaaaacta ttatctttcg ttttattttt
     7801 atctcaccat gacgcagtat caattgaaac aaaacgaaag atcaatatcg cagtaatctg
     7861 aactggagag gaaagtgaaa catctgacag aaatggtgag acagcacaaa gcgggcaaaa
     7921 caaatgcaat ttatgccgtt tgttccgcac atccgctggt gctggaggct gcaatccgct
     7981 acgccagtgc aaaccaaacg ccgttactga ttgaagcaac ctccaatcag gtagaccagt
     8041 tcggcggtta taccggaatg acgcccgccg attttcgcgg ctttgtttgt cagctcgccg
     8101 actcgttgaa tttcccgcag gatgcgctga ttctgggtgg tgaccatctg gggccaaacc
     8161 gctggcaaaa cctgccagcc gctcaggcaa tggccaatgc cgatgatttg attaaaagct
     8221 acgttgcggc aggattcaaa aaaatccacc ttgattgcag catgtcctgt caggacgatc
     8281 caattccctt aactgatgac atcgtggctg aacgcgccgc ccgtctggcg aaagtggcgg
     8341 aagaaacctg tcgcgaacac tttggcgaag ccgatctgga gtatgtcatt ggtaccgaag
     8401 tgccggtacc tggcggcgcg catgaaacct taagcgagct ggcggtcacc acgccggatg
     8461 ccgcccgcgc cacgctggaa gcccatcgtc acgcctttga aaagcaaggt ttgaatgcca
     8521 tctggccacg cattattgcc ctggtggttc aacccggcgt cgaattcgat cacaccaacg
     8581 ttattgatta tcagcccgcc aaagcgagcg ccttaagcca gatggtcgaa aactacgaaa
     8641 cgctgatttt cgaagcgcac tctaccgatt atcaaacgcc gcaatctctg cgccagctgg
     8701 tgattgacca ctttgccatt ctgaaagttg gcccggcgct gaccttcgcc ctgcgtgaag
     8761 cccttttttc tctggcggcg attgaagaag aactggtacc agcgaaagcc tgttccggtc
     8821 tgcgtcaggt gctggaaaac gtgatgctcg accgcccgga atactggcaa agccactacc
     8881 acggtgacgg caacgcgcgt cgtctggcgc gtggttacag ctactcagat cgcgtgcgct
     8941 attactggcc ggacagccag attgatgacg ctttcgctca tctggttcgt aatctggcgg
     9001 attcaccaat tccgctaccg ctcatcagcc agtatctgcc gctgcagtac gtgaaagttc
     9061 gctccggcga gctgcaaccg acgccacggg aactcattat aaaccatatt caggacatcc
     9121 tggcgcagta ccacacagcc tgtgaaggcc aataagcaaa acaaagagga acatgctatg
     9181 ccaaatattg ttttaagccg gattgatgaa cgcttgattc acggtcaggt cggcgttcag
     9241 tgggtcggat ttgcgggggc aaatctggtg ctggtagcca acgatgaggt tgccgaagat
     9301 ccggtacaac aaaacctgat ggaaatggta ctggcagaag ggatcgccgt acgtttctgg
     9361 acgctgcaaa aagttatcga caacattcat cgcgccgccg atcgacagaa aatcctgctg
     9421 gtttgtaaaa cacccgccga tttcctgacg ctggtgaaag gtggcgttcc ggtgaatcgc
     9481 attaacgttg gcaatatgca ctacgccaat ggcaaacaac aaatcgccaa aacggtttct
     9541 gtggatgcgg gcgatatcgc agcatttaac gacctgaaag ccgctggggt ggaatgcttc
     9601 gttcagggcg tcccgacaga gcctgctgtg gacctcttta aattactttg agggattcat
     9661 catggaaatc agcctgttgc aggcatttgc gttgggcatt atcgccttta tcgctggcct
     9721 agatatgttt aacggcttaa cccatatgca ccgcccggtg gtcctcggcc cgttggtcgg
     9781 gctggtactt ggcgatctgc ataccggaat tttaaccggc gatacgctgg aactagtgtg
     9841 gatggggctg gccccgctgg cgggcgcaca gccgcctaac gtgattatcg gtactatcgt
     9901 cggcacggcg tttgccatta ctactggcgt gaaacccgat gtcgcggtcg gtgtcgccgt
     9961 acctttcgct gtcgcagtac agatggggat taccttcctg ttctcggtga tgtctggcgt
    10021 gatgtctcgc tgcgaccgga tggcggagaa cgccgatact cgcggcattg aacgcgtgaa
    10081 ctatctggca ctgctggcac tcggcacttt ctattttctc tgcgctttcc tgcctatcta
    10141 cttcggcgcg gaacatgcaa aaaccatcat tgatgtcctg ccgcaacgat taatcgacgg
    10201 cctcggcgtc gcgggcggca tcatgccagc aatcggtttt gccgtgctgc tgaaaatcat
    10261 gatgaaaaac gtctacatac cctacttcat cctgggcttt gttgccgcaa cctggctcaa
    10321 gttaccggtg ctggctattg ctgccgccgc gctggcgatg gcgctgatcg atctgctgcg
    10381 taaatctcct gaaccgactc aacctgcggc acagaaagag gaatttgaag atggcatcta
    10441 atcaaaccac cctgccgaac gtcagcgaaa atgaagaaac actgctgact ggcgtcaatg
    10501 aaaacgtgta tgaagatcgg agcattgccg cggagctgac gaaaaaagat atcaaccgcg
    10561 tcgcctggcg ttccatgctg ttacaagcgt cttttaacta cgaacgtatg caggcttctg
    10621 gctggttgta cggtctgctg cctgctctga aaaagatcca cactaataaa cgcgacctgg
    10681 cgcgcgccat gaaggggcat atgggtttct tcaataccca tccgtttctg gtgacgtttg
    10741 ttatcggcat tatccttgcg atggagcgtt ctaagcagga cgttaacagt attcagagca
    10801 ccaaaattgc cgtcggtgcg ccgcttggcg ggattggcga tgcgatgttc tggctaacgc
    10861 tactgccgat ttgtggcggg ataggcgcaa gcctcgcgct acaaggctct attcttggcg
    10921 cagtcgtctt tattgtgctg ttcaacgtgg tacacttggg gctgcgtttt ggtctggcgc
    10981 attatgctta ccgcatgggc gtggcggcga ttccccttat taaagcgaat accaaaaaag
    11041 tcggccatgc tgcgtcaatc gttggaatga cggtaatcgg cgcactggtg gcaacctatg
    11101 ttcgtttaag caccacgctg gaaatcacgg cgggcgacgc agtagttaag ttacaggctg
    11161 atgttatcga caaactgatg cctgccttct taccgctggt ctacaccctg accatgttct
    11221 ggctggtacg ccgcggctag agtccgctgc gcctgattgc ggttaccgtg gttctaggga
    11281 tcgtcggtaa attctgccat ttcctttaaa tacaatgagg tattagatgt taagtattat
    11341 tttgacaggg catggcgggt ttgccagtgg catggaaaaa gcaatgaagc aaatcctcgg
    11401 cgatcaatcg cagtttattg ccatcgattt tcccgaaacc tcaagcgccg cgctgcttac
    11461 atcgcagctt gaagaggcca tcgcccaact ggattgtgaa gacggcattg tttttctgac
    11521 ggatttgctg ggcggcacgc cgtttcgcgt agcttcaacg ctggcgatgc aaaaaccggg
    11581 ctgtgaagtg attaccggca ccaatttgca actgctactg gagatggtgc tggagcgcga
    11641 agggttaagc ggtgaagaat tccgtgtaca ggcgctggag tgtggacacc gagggctgac
    11701 cagtctggtg gacgagttag gccgctgcca tgaagaatgt ccggtcgagg aaggaatatg
    11761 acacacgttc tgcgcgccag aaggctgctg actgaagagg gatggttcga tgaccatcag
    11821 ttgcgtgttg ctgacggtgt catcgcagca atcgaaccga ttccagtggg cgtgactgaa
    11881 cgcgatgcgg aacggctctg ccccgcttac atcgacaccc atgtacacgg tggtgcgggc
    11941 gttgatgtaa tggatgacgc gccggatgta ctcgacaagc tggcaatgca caaggcacgc
    12001 gaaggtgtcg gcagttggtt accgaccacc gtaaccgcgc cgcttaatac cattcatgcg
    12061 gcgctgaaac gtattgctca acgttgccaa cgcggcggac ctggtgcgca agtgctgggg
    12121 agttatctcg aaggaccgta cttcacgccg cagaataaag gcgcgcatcc g
//
LOCUS       DAJVQR010000108        12118 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVQR010000108 DAJVQR010000000
VERSION     DAJVQR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="OQM52_003329"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="OQM52_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS1565055.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="OQM52_003330"
     CDS             complement(2051..2974)
                     /locus_tag="OQM52_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1565056.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="OQM52_003331"
     CDS             3191..4534
                     /locus_tag="OQM52_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1565057.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="OQM52_003332"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="OQM52_003332"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1565058.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="OQM52_003333"
     CDS             6554..6778
                     /locus_tag="OQM52_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1565059.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="OQM52_003334"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="OQM52_003334"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1565060.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /locus_tag="OQM52_003335"
     CDS             complement(7372..8352)
                     /locus_tag="OQM52_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565061.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="OQM52_003336"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="OQM52_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1565062.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="OQM52_003337"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="OQM52_003337"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1565063.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10361..11017
                     /locus_tag="OQM52_003338"
     CDS             10361..11017
                     /locus_tag="OQM52_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCS1565064.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="OQM52_003339"
                     /pseudo
     CDS             complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="OQM52_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11549..12118
                     /locus_tag="OQM52_003340"
                     /pseudo
     CDS             11549..12118
                     /locus_tag="OQM52_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3138 a         2836 c         3043 g         3101 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ctgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcagacatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tataacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc
    10501 tgttttacgt tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa
    10561 cccaatcact tattatccgg taccaaaacc ttctacccag gtagggcaga aagttctgga
    10621 taaaattttg aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt
    10681 ggttaccacc gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac
    10741 cagcaaagaa gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac
    10801 gcaaatggct acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat
    10861 tgatgcagcg actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct
    10921 gaataacgaa agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc
    10981 gaccgatgcc accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat
    11041 ggcgcttttt atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg
    11101 gttttccacc tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg
    11161 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
    11221 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
    11281 gtttaccaga ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc
    11341 tggttcgcca tccatttcaa ccagttcagc ttcaaaataa gcacatacat ttgaaaagta
    11401 agtgcgtagt ttttctgtcg cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa
    11461 gactaggtga acatgcatca ggaaaacaca atgccttcca cgccggatat cggtttcttt
    11521 tttcacagac caaagtatca tttgacctgt gaaacgatta caggcattta aattccagtt
    11581 aagaccaggt ggtcaacagg agtgtgaaat gaggcgcttc gcaggcgcat gtcgtttcgt
    11641 tttcaatcgt gctctggcac gtcagaatga gaatcataag gccggtaata aatacatccc
    11701 ttacgggaaa atggcttcct ggctggttga gtggaaaaat gccactgaaa cgcaatggct
    11761 taaagattct ccctcacagc cattgcaaca gtcactgaaa gaccttgagc gggcttacaa
    11821 aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggtgac agaatgatgc
    11881 attccgctac ccgcagggcg ttaagctcga tcaggaaaac agccgtattt ttctgccgaa
    11941 actgggctgg atgcgctacc ggaatagccg tcaggtcacg ggtgttgtga aaaatgtcac
    12001 tgtcagccag tcctgcggta cgtggtacat cagtattcag acagaaagtg aagtatccac
    12061 tccggctcac ccttcagcat caatggtcgg gctggatgct ggcgtggcta aactcgcc
//
LOCUS       DAJVQR010000109        12038 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVQR010000109 DAJVQR010000000
VERSION     DAJVQR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(383..1027)
                     /gene="cheZ"
                     /locus_tag="OQM52_003341"
     CDS             complement(383..1027)
                     /gene="cheZ"
                     /locus_tag="OQM52_003341"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1565065.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1038..1427)
                     /gene="cheY"
                     /locus_tag="OQM52_003342"
     CDS             complement(1038..1427)
                     /gene="cheY"
                     /locus_tag="OQM52_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1565066.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1442..2491)
                     /gene="cheB"
                     /locus_tag="OQM52_003343"
     CDS             complement(1442..2491)
                     /gene="cheB"
                     /locus_tag="OQM52_003343"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS1565067.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2494..3354)
                     /gene="cheR"
                     /locus_tag="OQM52_003344"
     CDS             complement(2494..3354)
                     /gene="cheR"
                     /locus_tag="OQM52_003344"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1565068.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3373..4974)
                     /gene="tap"
                     /locus_tag="OQM52_003345"
     CDS             complement(3373..4974)
                     /gene="tap"
                     /locus_tag="OQM52_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS1565069.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5020..6681)
                     /gene="tar"
                     /locus_tag="OQM52_003346"
     CDS             complement(5020..6681)
                     /gene="tar"
                     /locus_tag="OQM52_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCS1565070.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6826..7329)
                     /gene="cheW"
                     /locus_tag="OQM52_003347"
     CDS             complement(6826..7329)
                     /gene="cheW"
                     /locus_tag="OQM52_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS1565071.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7350..9314)
                     /gene="cheA"
                     /locus_tag="OQM52_003348"
     CDS             complement(7350..9314)
                     /gene="cheA"
                     /locus_tag="OQM52_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCS1565072.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFALGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9319..10245)
                     /gene="motB"
                     /locus_tag="OQM52_003349"
     CDS             complement(9319..10245)
                     /gene="motB"
                     /locus_tag="OQM52_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS1565073.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10242..11129)
                     /gene="motA"
                     /locus_tag="OQM52_003350"
     CDS             complement(10242..11129)
                     /gene="motA"
                     /locus_tag="OQM52_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS1565074.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11256..11834)
                     /gene="flhC"
                     /locus_tag="OQM52_003351"
     CDS             complement(11256..11834)
                     /gene="flhC"
                     /locus_tag="OQM52_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1565075.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11837..>12038)
                     /locus_tag="OQM52_003352"
     CDS             complement(11837..>12038)
                     /locus_tag="OQM52_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1565076.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2759 a         3376 c         2917 g         2986 t
ORIGIN      
        1 gaccgactta aaccgcagac atcttgaact tgttcttcat actaacccca atccaagcgg
       61 ttcggtaacg aagattttac gcactatttt tactaagcag gtaaagagtt tagggccgga
      121 actctgtcat ttcttgcacc atgaactgac gaaaaaacgt tatgaagtat ggcatcacta
      181 cgatgtactg cactaccagt catcgtcatt ttctcgttca caatttaatg cattgataaa
      241 tttactggcg caacaatcac gcttcgctga tctaaaatta tcaaactaca aataaccatc
      301 ctccacgtac tccctgaacg atacccgcca ccctgctatc ggcttggcat catcgcgcag
      361 gccgaagttc aggcaataca aatcaaaatc caagactatc caacaaatcg tccacctgat
      421 cctgactggc taccacgccg gctttgctgg tatcgacctg aggtccatta agcaaactct
      481 ggttttcacg ttttggacgc gactcctgtt ccgggatgtt ttccaacagc accatcagca
      541 actggcgttc gatctcctga atgacatcca tcatccgctt aataacctgc ccggtgagat
      601 cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc gttagtaaag ctggtatgcg
      661 cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag ttcacgggcg tcggcaaggt
      721 caatcggatc ggcaaaccag tcatcccaac gttgggttaa cgcttttgct gatttctcca
      781 tttgatcctg atgcggttgt gacgcctcaa cactgttcag cgcccgctcc gcagcctggg
      841 cggtcatctg cacaacatag tacaaacgat cgcgcgcatc ggggatggct tccgccgctt
      901 cggcaatggc ctgaaccagc cccagttccc gcaaactgtc gcgcagcata tgcgtcaggc
      961 tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc agcaggtttg attgatggtt
     1021 gcatcatagt cgcatcctca catgcccagt ttctcaaaga ttttgttgag tttttcctcc
     1081 agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg cccccgcttg cgccgcagca
     1141 atgatgttct ctttcttcgc ttctgcagtc accattaaca ctggcaatgc cgacatcgcg
     1201 ccatccgcac gaattgtttt cagcaattcc aggccatcca tattgggcat gttccagtcg
     1261 gagataacaa atccataacc gcctgcctgc aacttattga gagcgtcgac accatcttcc
     1321 gcttcctcaa cattattgaa tcccagctct ttcagcaggt tacgcactat gcgtcgcatg
     1381 gtggaaaagt catccacaac caaaaattta agttctttat ccgccatttc acactcctga
     1441 tttaaatacg tatcgcctgt ccggcactaa ttgttgccaa catttgctgg cttacctggc
     1501 taagatcgac cacttcgcag acaccaccca tattgatggc ctcgcgcggc atgccgaaca
     1561 ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc cgcctgacgc atcgccaaca
     1621 ttcccgccgc gccgtcgttg cccataccgg tcaggatcac cccaaccgca ttacgccccg
     1681 cctgtttggc gacagaatgg aacaacacat ctaccgaagg ccgatgacgg ttaaccgccg
     1741 ggccgtcgtg aattttgatt tgataatttg cgccactacg cgccagttcc atatgccgat
     1801 cgcccggcgc aatataggca tgccccggca agacacgttc tccgtcttcg gcttctttaa
     1861 ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga gcgggtgaaa ccgggcggca
     1921 tatgttgggt aattaacaat gccgggctgg aaagcggcaa cggttgcagt acgtgacgaa
     1981 ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag tttttcagaa ctcaacaacg
     2041 gccctgcctt cagcgttgtc ggtgctgaca atggcttatg tgctgcaagg ctcgcctttg
     2101 ctgccgtacg caccttttca gcaatcattt cgctatacgc cagcattcct tcgcgaatac
     2161 ccagttgcgg tttggtgaca aaatctattg cccccagctc cagcgcgcgc agcgtgactt
     2221 ctgatccttt gccggtcaag gaagaaacca tcacaacggg cattggacgc aaacgcatta
     2281 atttttcgag gaaatccagt ccgtccatcc gcggcatttc aacatccagc gtcagcacat
     2341 cgggattgaa tttcttaatc aagtcacgcg caaccagcgg atcaggcgcg gtcgccacca
     2401 tttccatgtc gctatggctg ttgatgattt ctgtcatgat ctggcgcatc agtgccgaat
     2461 catcgacaga taacaccctg attttgctca tcgttaatcc ttacttagcg catacaccgt
     2521 ctgaccacgc agcgtgaagc ggcgctcgag gtggctaaag ttttcagagt gacccgcaaa
     2581 cagcaatccg tcgggtttaa ggagcggaac aaagcggcgc aaaatctcct gctgggtagt
     2641 ttgatcgaag tagatcatga cgttacgaca gaagatcgca tcaaacggcc ccggcacggt
     2701 gtactgtttc gccagtaaat tcagcggggc aaaatcaaca tagttcgcca gctcctgacg
     2761 cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg aaataccgtt gcagttgctg
     2821 cggcgtcagg tttttcaact cttcatggcg atagataccg cttctggctt tttccagcac
     2881 ttcggtgtcg atatcactgg caaacacttt ccagcgtccg ggcacggtgc ccaatgtgtc
     2941 agccagagtc atcgcaatgc tgtacggctc ttcgccggtc gaagccgccg cgctccatac
     3001 gcgatcctcg ccagaacggc gacgtgcgtg atccgcgagc agagggaaat gatgagcctc
     3061 acggaaaaat gccgtcagat tcgtggtcag cgaattgata aacgcctgcc actcaccgct
     3121 gtgctgatta gattccagca agttcagata atgaccgaaa tccgtcagtc ccagagaacg
     3181 caaacgacga accagtcggt tgtaaaccat gtcgcgttta tggtcagcca gaacgatccc
     3241 ggctcgttga tagatcaatt gacttatccg ccgaaaatgc gcgtcggaaa gcgccaggcg
     3301 ctcggtcatc tgtaacaata aagacgtttg cccacagggc agagatgaag tcatagcgcc
     3361 ttctcaatca cttcaggata ccactggcgc aatttgtaac tgcgccgact catgtcgtgc
     3421 tacttcatgt tcttcaaggg taaataccgc cacacgcgac gaaagatggt cggcctgatt
     3481 cgccagttgt tccgttgcca ccgccgcttc ttctaccagc gaggagttct gctgagtcac
     3541 ctgatccatc tggctgacag cctgtgcaac ctgctcaatc ccccgccgtt gttcttccga
     3601 cgccgaagca atttctccca taatgtcgtt cacgcgggtc accgaactga caatatcggt
     3661 catggtcgcg gcggcgttat tcaccagttt cgaaccctgc tggacacgat tgactgactc
     3721 ttcgatcagc cctttgatct cttttgctgc ctgcgcgcta cggctggcaa gattgcgcac
     3781 ttcacctgcc actaccgcaa aaccacgccc ctgctctccg gcgcgagccg cttccactgc
     3841 cgcattcagg gccagaatat tggtctggaa agcaattccg tcgataacgc tgataatgtc
     3901 gccaattttt tgcgagctgg tggcgatctc ctgcatggtg tgagtcatgg tactgacctg
     3961 aacaccgccc gcctgcgccg ttgtcgcggc attttttgcc agttccgacg cctgtcgtgc
     4021 gttatcggcg ttttgcccta ccgtggcggt taattgctcc atactggcgg ccgtttgtgc
     4081 cagcgatgcc gcctgctgtt cggtacgact tgagagatcg ttattgcctg cgacaatctc
     4141 cgcgatccca atgtgcatct catggcttcc cttacgcaca tcactcaccg tcccacgcaa
     4201 agcctgctgc atggtcttca ggctggcaaa aatggcggtg atctcattac gaccatatac
     4261 cgcaatcgga cgcgccagat tacccgcagc aatgctgtca aaatggctac cgataatggc
     4321 cagtggttga acaatcatct tgcgcgtcca ccacagcgca ctgctgatgt agatcgccgc
     4381 aacaataatc atgctgataa acaccagcgc cgaaatctga tagttacgct ggctttgcgc
     4441 actggcggct tccagcacat ggttgatctc cagctgccag gcctcaaagt taacgtcaaa
     4501 cgcattctgt gatccctgca ccggcgcagt gaggaaatcc gaaagttgat tactttcaag
     4561 ccaggtcgcc tgatgttcga gatcgttgtg ccagcgggca aaacttttct ccgtctcttt
     4621 ttgcaatgcc ctgacgtgct cgttgcccgc agtcatcgcc ataaaacttt taaacagcgt
     4681 ggtggattgc gtcagactgg cgcgcgccgt cgtcatcaac gttttaatgt catccgccgg
     4741 atagctaagc gctgtcagag tgcctgcttt gttcagcgcg gtactggcct gtaacattac
     4801 cgcccgagtt tgcgctaatg ccgcacgttg ctgattgctc tgctcgccgt gattcagtcg
     4861 ttgcaaatcg tcgcgaaatg cccaaaaaga catgccgtta ctgccaatct gcaagatccc
     4921 gcagagaatc aaaattaaaa acagcgtggt cgaaattcga atacgattaa acatcaacgc
     4981 tccccatcag gcggcaatga ccgcgttagt aaaaactcgt caaaatgttt cccagtttgg
     5041 atcttgttca gtaattcgca gtcgtggctg tgccggtggt tgctcactgg caggacggga
     5101 tggtgtttgc ggtttattgg tgagtgggct ggctgccaga cggaacgcgg aaaccgcttg
     5161 cgttaaacga ctcgcctgtt cttccagcgc agctgcggcg gcagctgatt cctgcaccag
     5221 cgatgcgttc tgttgcgtga cgcgatccat ttccgaaacc gccaatgcga cttgatcgat
     5281 gccacggctc tgttcatccg atgccgatgc aatctcgccc ataatgtcag tcacgcgagt
     5341 gacagcattg acgatattgt tcattgtttc cccggcgctt tcgaccagca ccgaaccggt
     5401 atcaatgcgc gagacggagt cttcaatgag ggctttgatc tcttttgccg ccagggcgct
     5461 gcgactggca agattacgca cttcacccgc caccacggca aaaccacggc cctgttcacc
     5521 cgcacgcgcg gcttcaaccg cggcattcag cgcgaggata ttagtctgga aggcaatacc
     5581 gtcgataacg ctgataatgt cggcaatttt cttcgaacta tcggcgatct catgcatcgt
     5641 tttcactacg ccatccacca ctttgccgcc gtgctgggcg gtgtcggagg cactttgcgc
     5701 cagttgcgag gcctggcggg cgttatcggc gttttgcttc actgtcgcgg tgagctgctc
     5761 catgctggcg gcagtttctt ccagcgcgga tgcctgctgt tcagtacggg aggaaagatc
     5821 ggtgttgccc gccgcaattt cacgggtacc ggcatagatg gcatctgaac cttcgcggac
     5881 atgagtgacg gtgtcagtca aagagcgttg catatgtgaa acgctctgcg ccaggtcacc
     5941 catttcactg cgcccgtcaa tggtcagggt attcgccagg ttaccaccgg cgatttcgca
     6001 aatgtgagca ataatttttg ccagcggagt aagcaacata cggcgaatgc cgtaccacgc
     6061 caccagcaga atcaatacca ccaccagcgc gataaccgcc agttgccact gggcaaatcg
     6121 gtaatcatct gcgttgtcag tgacgatatc gcgatacagt ttttcactgc tgagggcgta
     6181 ctgagcaaac gcttcgccca ttgcattttg cattccctgg gttggctgag cgaaataagc
     6241 tccagtattg ccataatcaa gataatcaat cagttcagtt aacgctgtgt aatagttttt
     6301 atatttttca tcaaaattac ggctggtagc gaccatttca ggtaacggtg ccatgctttt
     6361 gaatttttta taatgcgtcg cggcctgggc caatgttttc ctggcgctat cgagcaattc
     6421 aactttggcg ttactttgtt gattggagga atccatcatc atccgcaccg ctgaacgact
     6481 caggttaatg cgcgtttgca gcattaaatc ccaggttgac gtcagctcgc cctgctgttc
     6541 ccgtaattga ttggaaacca caaagctctt ctggctatgg tgaagggaag aaaaaaacaa
     6601 actgccggaa ataagctgta acagtgcgaa tacccccagc accattacca acagcgtgac
     6661 tacgcggata cggttaatca taaggcacct tcctgaaaac aagttgatct cgttatcggc
     6721 aagaaggggg gaaactttat tgctgatgcc acccgccgcg aaattgaaat aaaaaacccg
     6781 atgcgcagat catcgggttc atttcaattg aggaaatcgg gagaattacg ccacttctga
     6841 cgccgcgcta tctaacagcg ccatctcttc gctgttcagc agtttttcga tgttcaccag
     6901 aatcaacata cggtcgccca gtgcgcccag tccagtgaga tattctgttg aaagcgtcac
     6961 ggcaaactct ggtgccggac gaatttgctc cgccgtcaat gaaagcacgt ctgaaacgcc
     7021 gtcaaccacg atgccgacca cccgctgtcc gagattcagg acgataacta ccgtgttgtc
     7081 gttatagtcc acatccacct ggctgaactt aattcgtaag tcaacaatcg gcacaataac
     7141 gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg ttcgcaatcc gtgttacctg
     7201 atcgtagcca cggatctcct gcactttcag gatatcaata ccgtactctt catcaccaag
     7261 ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc agctttgtta cattcgtcat
     7321 accggtcata ttgttacctt tttactcatt caggcggcgg tgttcgccat acgttgttcg
     7381 cggtttatcg cctgcaaggc ggagacatca acaatcagtg ccacgctgcc gtcgccaaga
     7441 atggtcgcag cagaaatgcc ggggactttg cgatagttac tttcaaggtt tttaaccaca
     7501 acctggtgtt gaccaattaa ttgatccacc agcaaggcgt agcggcgacc gccactttgt
     7561 aagatcacca caattccctg ggtggcttcg gttttcgcgc ccgcgacgtt gaacactttc
     7621 cacagttcga cgatgggcag atattcaccc cgcacttcca gcacccgctc gccgccggcc
     7681 agtggatgga gatcggcttc acggggttgc agtgattcca taacagcatt cagcggcaga
     7741 atgaaaactt catccgcaac gcgtacggac atgccgtcga ggatggccag cgtcagcggc
     7801 agtaaaatgc ggatcgtagt gccagtaccc tgcttcgact ggatttcgac atgaccgccc
     7861 atctcctgga tattacgttt aacgacgtcc atgccgacgc cgcgcccgga gacgtcggtg
     7921 acctgctctg ccgtggagaa gcctagtgca aatatcagca ttgcaacttc gtcgtcgctc
     7981 atgttttcgc tgacagtcaa accttgcgag gccgcttttg ccagaattcg ctcacggttt
     8041 agccccgccc catcgtcggt cacttcaatg caaatgttgc cgccctgatg ttcggcagac
     8101 agaattaaat ttccgacgct gtttttacct gcggcgagcc gtttttctgg cagttcaata
     8161 ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt cgataatgcg ttctatcagg
     8221 ctcttgtcga gctcggtgga actgcccacc agcgtcagtt ctacctgctt gccgagtttt
     8281 cccgccagat cacgcaccag ccggggatag cgactaaaga catattccat cggcatcatg
     8341 cgaatcgaca tcactgattc ctgcaaatca cgggcgttac gttgtaactg ccccatgctg
     8401 gtaatcaaat caccatgatt aaccgggtcc agttcgctgg aacgctgggc aagcatggac
     8461 tgggtgataa ccagctcgcc gacgaggtta attaattgat caaccttttc taccgctaca
     8521 cggatgctgg tggattcact gctgcgcgtc gttttttccc gctccacgcg gccggttggc
     8581 gcttgttcgg ctgccagttt aagcacaggt ggggtagcta tttttggcga aacttctact
     8641 gtttcaaagg taatctgatc ggcttcaatc acaaaacaga gtaccgctgt gatgtcatct
     8701 tcggcgatat cgcccggtaa tattgccgag agagaatccg cccctttcac cacgtcattt
     8761 aacgttgtca gatgccccag ctcttcttcc agcaggtcga cttcgccggc cttcaggcgc
     8821 gaaaggataa ttcgtcgcgg caactgactg cgactttgct catcttgcgg ttcacttttg
     8881 gcaaccacac ttaatcgggt cactgcggat ggcgtttcgc ctttcgcttc taatgccagt
     8941 tgacgcaagg cctggcagat ataatcgtag ctggctgcat ccggctcttg cgactgttta
     9001 taagcgtcga gctgttcttg catgatgtcc ttcgtttcca aaaacagatt gataatgtcg
     9061 gtgttgagtt gcatctcacc tcgtctggct tcatcgagca ggttttccat cagatgcgtg
     9121 gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc ctttgatcga gtgggcagcc
     9181 cgaaagatgg cattcaattg ttcggcatct ggcgcttccg gctgcaaaac cagcagatgc
     9241 tgctccatat cagccaacag ttcgtccgct tcatcaaaaa atgtctgata aaaatcgctt
     9301 atatcgatgc tcacgctgtc acctcggttc ggctgatggc attgtgggaa cactgacctg
     9361 tggtgcaacc tcaggttttt ctagggcgct tactggctca ttctggcttt cggcgttttc
     9421 atgcaaaatg gcctgttcgg cttgtttgtt cagtaccaac aggctgatgc gacggttgac
     9481 ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc gccatgccga cgacacgtaa
     9541 cactttgccg ctatccaacc ctccgaccat cagttcgcgg cgggatgcat tggcccgatc
     9601 ggcagaaagc tcccagttgc tgtaaccttt ctcaccgctg gcgtagggaa aatcatcggt
     9661 atgacctgaa aggctaatac ggttgggaat accgttcagc acaggcgcga tggcgcgcag
     9721 aatgtcgcgc atatagggtt cgacatcggc actgccggtt ctaaacatcg ggcgattctg
     9781 gctatcgatg atctgaatac gtagaccttc ctggaccaga tcgattttga gatggagacg
     9841 taacgcccgc agtttcggat cggactctat caactggtcg agatcacccc gcaatttccg
     9901 caatcgactt tgctccatgc gttttttcag ctcttcgatg ttcggctgct tattcacttc
     9961 cccctggctt tgggtgtaat catcaccacc gccgggaatt gggctttcac tattggaaat
    10021 gcgatcgccg cccgtaaccg ctgtcgccag tggagtccgg aagtactccg caatctgaat
    10081 caactctttt gggctggaga tggagatcag ccacatcacc agaaaaaagg ccatcatcgc
    10141 agtcataaag tcggcataag caatcttcca cgatccatgt gctgccccgt ggcttttggc
    10201 tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc ttcatgcttc ctcggttgtc
    10261 gtctgttgtt gcggattttt caccgcacgc acatgctctt ccagttcaat aaacgacgga
    10321 cgttcgctgg aatagagcgt tttgcgacca aactcaacgg cgataggcgg tgcgtaaccg
    10381 ttcagattag aaagcagagt gactttgacg cactgcatca ttttgctggt ttcggcgctt
    10441 ttctgacgta aaacactcgc tagtggggaa ataaatccgt aagccaataa aataccgagg
    10501 aaagtcccca ccatcgcatg tgcgataagc gcccccagct cggcggcagg acgatcggct
    10561 gaacctaacg cgtgaacgcc ccccattaca gccgcgacaa taccaaacgc cggaagtgag
    10621 tccccgacca gcgccagact gttcgccggg acttctgcct cgctttcgtg cgtctcaatc
    10681 tcttcatcca tcagggcttc gatttcgaag gtgttcatgt gaccgctgat aatcaggcgc
    10741 agataatcga cgataaaatc aagcatgacg ctatccgcga ggatgcgtgg gtagctggcg
    10801 aagatctcgc tctcacgggg attttcaata tcacgttcca gcgaaaacat ccccatctgc
    10861 cgcgatttcg ccatcaaccg ataaagcaga gccagcagat ccatatacat tgctttggtg
    10921 tatttggagc gacgaaacag caacggcagc gctttcagcg tgcctttaat cgctttgcca
    10981 ttattgccga cgataaacga cccaatccct gcaccggcaa taatcaccag ttcagcgggt
    11041 tgatagagtg ctccaaggct tccaccggtc atcaaataac cgccgaaaac tgtaccgaga
    11101 acaaccaggt aacctaataa gataagcacg acatcatcct tccactgttg accatgacag
    11161 gatgttcagt cgtcaggcgt taacgcgcga ttggggaaaa aaaaagcagc ggtacgtcgt
    11221 taccgctgct ggaatgttgc gccacaccgt atcagttaaa cagcctgtac tctctgttca
    11281 tccagcagtt gtgggataat atcggcagga ttctgggaaa gtttacgtct ttttactgcc
    11341 cgggatggcg gttgacataa gctgcaggca aagctgccaa caggctggtg agcgtgggta
    11401 ataaaattgc cgccgcagca gttgcagctg gaaagttgca gtaatccact ttcaacaaac
    11461 cgcaccaatg tccaggcacg ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac
    11521 tgttcaaggt ataaacggta ggctttgatc accgcatcga cgccattaca caaaccggtt
    11581 ttcagtaaaa actgccatgc attacagaac atcgaagcat gaacgttttg ttcccaggtc
    11641 ataaaccagt cggttgagaa tggcagcatg cctttcggcg gtgggcttcc gcgcagttct
    11701 ttataaagtt ttatcaggcg tccgcgactt aactgtgttt cgctttccag catctgcaaa
    11761 cgagcgccca gggtgatcaa ttccattgcc agctgaatat cccgcgcttc ctgaacaatg
    11821 cttttttcac tcatgatcag gcccttttct tgcgcagcgc ttcttcaggc tgattaacat
    11881 cattcagcaa gcgtgttgag agcatgatgc cggtatgaat ttgctggaga tcgtcaacgc
    11941 gggaatcttg cgtcaactga gtaatcgtct ggtggctgtc aaaacggaag tgacaaacca
    12001 gctgattggt ttctgccagc ttaaccattt gcggaaga
//
LOCUS       DAJVQR010000110        11925 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVQR010000110 DAJVQR010000000
VERSION     DAJVQR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..2342)
                     /locus_tag="OQM52_003353"
     CDS             complement(<1..2342)
                     /locus_tag="OQM52_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1565077.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OQM52_003354"
     CDS             complement(2276..2899)
                     /locus_tag="OQM52_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCS1565078.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OQM52_003355"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OQM52_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OQM52_003356"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OQM52_003356"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1565079.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OQM52_003357"
     CDS             7507..8229
                     /locus_tag="OQM52_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1565080.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OQM52_003358"
     CDS             complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OQM52_003358"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCS1565081.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10301..11896)
                     /locus_tag="OQM52_003359"
     CDS             complement(10301..11896)
                     /locus_tag="OQM52_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCS1565082.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3309 c         2945 g         2914 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg ccataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcgg
//
LOCUS       DAJVQR010000111        11688 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVQR010000111 DAJVQR010000000
VERSION     DAJVQR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            100..969
                     /locus_tag="OQM52_003360"
     CDS             100..969
                     /locus_tag="OQM52_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCS1565083.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1172..1525)
                     /locus_tag="OQM52_003361"
     CDS             complement(1172..1525)
                     /locus_tag="OQM52_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1565084.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1663..3309)
                     /gene="groL"
                     /locus_tag="OQM52_003362"
     CDS             complement(1663..3309)
                     /gene="groL"
                     /locus_tag="OQM52_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1565085.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3353..3646)
                     /locus_tag="OQM52_003363"
     CDS             complement(3353..3646)
                     /locus_tag="OQM52_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1565086.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3922..5178
                     /gene="yjeH"
                     /locus_tag="OQM52_003364"
     CDS             3922..5178
                     /gene="yjeH"
                     /locus_tag="OQM52_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1565087.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5194..5670)
                     /gene="fxsA"
                     /locus_tag="OQM52_003365"
     CDS             complement(5194..5670)
                     /gene="fxsA"
                     /locus_tag="OQM52_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1565088.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6007..7443
                     /gene="aspA"
                     /locus_tag="OQM52_003366"
     CDS             6007..7443
                     /gene="aspA"
                     /locus_tag="OQM52_003366"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1565089.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7561..8862
                     /gene="dcuA"
                     /locus_tag="OQM52_003367"
     CDS             7561..8862
                     /gene="dcuA"
                     /locus_tag="OQM52_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1565090.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8978..9316
                     /gene="cutA"
                     /locus_tag="OQM52_003368"
     CDS             8978..9316
                     /gene="cutA"
                     /locus_tag="OQM52_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1565091.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9292..10989
                     /gene="dsbD"
                     /locus_tag="OQM52_003369"
     CDS             9292..10989
                     /gene="dsbD"
                     /locus_tag="OQM52_003369"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1565092.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11026..11601
                     /locus_tag="OQM52_003370"
     CDS             11026..11601
                     /locus_tag="OQM52_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1565093.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2808 a         3020 c         2874 g         2986 t
ORIGIN      
        1 tatttatgtt attttaacaa ctattactac caagcccatt tatctggctt ataaattaaa
       61 attaattatt taattgagaa atggttaggg agaacctaca tggccataag catcaagggt
      121 gttaataccg gtgttattcg caagagtaat aatttcattg cactggcgct gaaaattaaa
      181 gaacctcgca ataaagaatc gttgttcttt atgtctgtca tggaactgcg cgacctgctg
      241 attgcgctgg aaagtcgtct gcatcaaaag cataaactgg atgctgctgc acgccttcag
      301 tatgagcaag ctcgcgataa agtcattaag aaaatggcag aaaacatccc agaaattctg
      361 gttgatgagc ttaaaaatgc cgatattaat cgtcgggtta acacgctgga acttaccgat
      421 aatcaaggcg aaaatcttac cttcgtatta acgctacatg acggtagcaa atgtgagtta
      481 gtcgtcaatg agttgcagat tgaaatgctg gcgcgagcaa tcattcatgc aattaataat
      541 gcggaaatgc gtgaactggc attacgtatt acctcactgt tagatttcct gccgttatac
      601 gacgttgact gccaggacaa tggcaatctg gaatatgaca cctattccca accggaatgg
      661 aaacataact tgttcgacca ttatctggcg gtgctttacc gctttaaaga tgaaagtggc
      721 aaagaacaat ttagcggcgc agtggtgaaa acgcgtgaag caacgccggg taaagaaata
      781 gaggcgatca ctcgccgaat gcttgatttc agcccacgac tgaaaaaact cgcgggtgtg
      841 ccatgtcagg tctatgttcg caccgtagcg gcgaataacg cccaaccgct aacccaggat
      901 caatgcttac gcgcgctgca tcacttacgt gtacaatcca ccagcaaaac tgcaccacag
      961 gcgaaataat ctcatctttg ctaatgcgag gctggagcca tccagcctga aatgactgat
     1021 aacgtgatcg ttggtcatgc cggatgcggc gtaaacgcct tatccggcct acaaattctc
     1081 aagcattcaa tcagttgcaa tgcctgctgt aggcctgata agcgaagcgc atcaggcaat
     1141 aatacgtttg tcacccagcc tctggatcac atcagttcgg gcacttataa acctgaccga
     1201 taatctggct atccgtcggg acaaaactgg acaacattcc ctgcgacggg ctgctgatgc
     1261 cataaatcac gttaccgccc attgcagccg cctggttgcg cagatcgttt gctgcgccgc
     1321 gcatagaacc gccctcttct ccgtgttgcc cggaaagcca gttgctttgc ttacctgtcg
     1381 cagtaccaat cagctggcac tctgcgcctg gctgctcgtc cacaatgcgt acactctgac
     1441 cggcagcact caattcgttg ctggagctac aacccgccat cagtagcgcg gcaccgacaa
     1501 tccctgctaa gtattttacg tgcatgttat tccccataat caatgagctg gacgccactc
     1561 gcgtcatccg tgtctgaatc ttatactaga aagatgacca aaagaaaaac ccccagacat
     1621 ttctgcccgg gggtttgttt atttctgcga agtgcagggc aattacatca tgccgcccat
     1681 gccacccatg ccgcccatac cgccagcagc gcctaagtca gctgcatcgt ttttcggcag
     1741 gtcggtaacc atgcattcgg tggtgatcat caggccagcc acagaagctg cgtactgcag
     1801 agcagaacgg gttactttgg ttgggtccag gatacccatg tcgatcatgt tgccgtattc
     1861 ttcggttgct gcgttgtaac cgtagttgcc gtcgccgcct ttaacggtgt tagcaacaac
     1921 agacggttct tcgccgcagt tcaggacgat ctgacgcagc ggagcttcca ttgcacgcag
     1981 tgcaactttg atacccacgt tctggtcttc gttctgacca cgcaggtcag ccagtttaga
     2041 cgctacgcgg atcagcgcaa caccaccacc agcaaccacg ccttcttcta ccgcagcacg
     2101 ggtcgcgtgc agggcatctt caacgcgtgc ttttttctct ttcatttcaa cttcggtagc
     2161 agcacctact ttgataactg caacgccgcc tgccagtttc gctacgcgct cctgcagttt
     2221 ttcacggtcg tagtcagaag ttgcttcttc aatctgctga cggatctgag caacacggcc
     2281 ctggattgca gcttcttcac ccacgccatc gatgatagtg gtggtgtctt tgttgatcac
     2341 aacacgttta gcctgaccca ggtcttccag ggttgctttt tccagctcca taccgatctc
     2401 ttcagagatt acggtaccgc cagtcagggt tgcgatatcc tgcagcatag ctttacgacg
     2461 atcgccgaag cccggagctt taaccgcagc aactttcacg atgccacgca tggtgttaac
     2521 aaccagagtt gctagcgctt cgccttcaac atcttcagcg atgatcagca gcggtttgcc
     2581 tgctttggca acagcttcca gaaccggcag catttcgcgg atgttggaga ttttcttgtc
     2641 agccagcagg atgaacgggc tttccagttc tactgcgcca gtttccggct tgttgatgaa
     2701 gtaaggagac aggtagccac ggtcgaactg cataccttca accacgtcca gttcgtcctg
     2761 cagaccggta ccgtcttcaa cggtgataac gccttcttta ccgactttgt ccatcgcttc
     2821 agcgatcagt ttacctacgg tttcgtcgga gttagcggag atggtaccaa cctgagcaat
     2881 cgctttagaa tcagagcacg gtacggacag cgctttcagt tcttcaactg cagcggtaac
     2941 cgctttgtcg ataccacgtt tcaggtccat cgggttcatg cccgcagcaa cagctttcag
     3001 accttcagtg atgatagcct gagccagtac ggttgcagtg gtggtaccgt cgcctgcagc
     3061 gtcgttcgct ttggaggcaa cttctttcac catctgcgca cccatatttt cgaacttgtc
     3121 ttccagttcg atttcacgag caacggaaac accatctttg gtgatggtcg gtgcaccgaa
     3181 agatttatcc agaactacgt tacggccttt tggaccgagg gtaactttca ctgcatctgc
     3241 cagtacgttt acgccgcgca gcattttcac acgagcgtcg ttaccgaatt ttacgtcttt
     3301 agctgccatt atctttattc cttaaattcg tatgttcagt gtcgtgcgcg gattacgctt
     3361 caacaattgc cagaatgtcg ctttcggaca tgatcaacac ttcttcattg tcgatcttct
     3421 cggatttcac accgtagcca tcgttgaaaa taacgatgtc gccaactttc acgtccagcg
     3481 gcttcacttc gccattttca aggatacggc cattgccgac agccagcact tcgccgcggg
     3541 tggatttagc cgctgcagag ccggtcagaa cgatgccgcc agcagattta gtttcaactt
     3601 ctttacgctt gacgatcacg cgatcatgca atggacgaat attcattgat aactctcctt
     3661 tgagaaagtc cgtatctgtt atgggtgacg ccggagctta cgtggtttcc cggctggtga
     3721 ccagagaaat ggggatgagg cttcgcccct tcaaggggga aaaagaaaaa aaattctgat
     3781 cagcacaaaa tcgggtgaaa accctgattc acctcacatt tcatcgcaat ttcttcatcg
     3841 ccgtcataag cgaatctgat tgtgctacca tcgaaaatct acgcagttgc ccaaaatttg
     3901 ggcgcaatcg gatatcaacc catgagtgga ctcaaacaag aactggggct ggcccagggc
     3961 attggcctgc tatcgacgtc attattaggc actggcgtgt ttgccgttcc tgcgttagct
     4021 gcactggtag caggcaataa cagcctgtgg gcgtggcccg ttttgattat cttagtgttc
     4081 ccgattgcga ttgtgtttgc gattctgggt cgccactatc ccagcgcagg cggcgtcgcg
     4141 cacttcgtcg gtatggcgtt tggttcgcgg cttgagcgag tcaccggctg gctgttttta
     4201 tcggtcattc ccgtgggttt gcctgccgca ctacaaattg ccgccggatt cggccaagca
     4261 atgtttggct ggcatagctg gcaactgttg ttggcagaac tcggtacgct ggcgttggtg
     4321 tggtatatcg gtactctcgg tgccagttcc agtgctaatc tacaaaccgt tattgccgga
     4381 cttatcgtcg cactgattgt cgctatctgg tgggcgggcg atatcaaacc tgcgagtatc
     4441 cccttccccg cgccaggaaa tatcgaactt accgggttat ttgctgcgtt atcagtgatg
     4501 ttctggtgtt ttgtcggtct ggaagcattt gcccatcttg cctcggaatt taaaaatcca
     4561 gagcgtgatt ttcctcgtgc tttgatgatt ggtctgctgc tggcaggatt agtctactgg
     4621 ggctgtacgg tagtcgtctt acacttcgac gcctatggtg aacaaatggc ggcggcagca
     4681 tcgcttccaa aaattgtagt acagttgttc ggtgtaggag cgttatggat tgcctgcgta
     4741 attggctatc tggcctgctt tgccagtctt aacatttata tacagagctt cgcccgcctg
     4801 gtctggtcgc aggcgcaaca taatcctgac cactacctgg cacgcctctc ttctcgccat
     4861 atcccgaata atgccctcaa tgcggtgctt ggctgctgcg tggtgagcac gttggtaatt
     4921 catgccttag agatcaatct ggacgctctt attatttatg ccaatggcat ctttattatg
     4981 atttatctgt tatgcatgct ggcaggctgt aaattattgc aaggtcgtta tcgactactg
     5041 gcggtggttg gcgggctgtt atgcgttctg ttactggcaa tggtcggctg gaaaagtctc
     5101 tatgcgctga tcatgctggt ggggttatgg ctgtttctgc caaaacgaaa aacgccggaa
     5161 aatggcataa ccacataatc cggcgtttcg acattaatcc tggcgatcgt ctttatgatc
     5221 aaggcggtcg cggtcatcat cctttcgctg atactcacca tcaaaagtat taccgccacc
     5281 ggtcccggcg ctaaaaccgc cgcctggcat gcgagaaaag cgcaaatgtg gcatcaactt
     5341 cactgtcaga tgcttttgca ccggcggcaa taaaagcaga agaccgagga agtcggtgaa
     5401 aaagcccggt aataaaagca gcaaaccggc aataatcagc gaaacacttt taatcatctc
     5461 cgccgcaggg ttttcacccg ccgccatttt ttgctgcatc agcacgaaat tcttaaagcc
     5521 ctggttacgt accagtgaca taccgataac tgacgtgaat ataaccagca cgagggtcag
     5581 caataccccc aatacatggg caacctgaat aaagattgaa atctcaatat agacataaag
     5641 gaaaatggca ataaaaggta accagcgcaa aggtttctcc tgtaatagca gccggttaaa
     5701 cccggctacc tgaatgggtt gcgaatcgcc ttcagcttat attgtggtca ttagcaaaat
     5761 ttcaagatgt ttgcgcaact atttttggta gtaatcccaa agcggtgatc tatttcacaa
     5821 attaataatt aaggggtaaa aaccgacact taaagtgatc cagattacgg tagaaatcct
     5881 caagcagcat atgatctcgg gtattcggtc gatgcagggg ataatcgtcg gtcgaaaaac
     5941 attcgaaacc acatatattc tgtgtgttta aagcaaatca ttggcagctt gaaaaagaag
     6001 gttcacatgt caaacaacat tcgtatcgaa gaagatctgt tgggtaccag ggaagttcca
     6061 gctgatgcct actatggtgt tcacactctg agagcgattg aaaacttcta tatcagcaac
     6121 aacaaaatca gtgatattcc tgaatttgtt cgcggtatgg taatggttaa aaaagccgca
     6181 gctatggcaa acaaagagct gcaaaccatt cctaaaagtg tagcgaatgc catcattgcc
     6241 gcatgtgatg aagtcctgaa caacggaaaa tgcatggatc agttcccggt agacgtctac
     6301 cagggcggcg caggtacttc cgtaaacatg aacaccaacg aagtgctggc caatatcggt
     6361 ctggaactga tgggtcacca gaaaggtgaa tatcagtacc tgaacccgaa cgaccatgtt
     6421 aacaaatgtc agtccactaa cgacgcctac ccgaccggtt tccgtatcgc agtttactct
     6481 tccctgatta agctggtaga tgcgattaac caactgcgtg aaggctttga acgtaaagct
     6541 gtcgaattcc aggacatcct gaaaatgggt cgtacccagc tgcaggacgc agtaccgatg
     6601 accctcggtc aggaattccg cgctttcagc atcctgctga aagaagaagt gaaaaacatc
     6661 caacgtaccg ctgaactgct gctggaagtt aaccttggcg caacagcaat cggtactggt
     6721 ctgaacacgc cgaaagagta ctctccgctg gcagtgaaaa aactggctga agtcactggc
     6781 ttcccatgcg taccggctga agacctgatc gaagcgacct ctgactgcgg cgcttatgtt
     6841 atggttcacg gcgcgctgaa acgcctggct gtgaagatgt ccaaaatctg taacgacctg
     6901 cgcttgctct cttctggtcc acgtgccggc ctgaacgaga tcaacctgcc ggaactgcag
     6961 gcgggctctt ccatcatgcc agctaaagta aacccggttg ttccggaagt ggttaaccag
     7021 gtatgcttca aagtcatcgg taacgacacc actgttacca tggcagcaga agcaggtcag
     7081 ctgcagttga acgttatgga accggtcatt ggccaggcca tgttcgaatc cgttcacatt
     7141 ctgaccaacg cttgctacaa cctgctggaa aaatgcatta acggcatcac tgctaacaaa
     7201 gaagtgtgcg aaggttacgt ttacaactct atcggtatcg ttacttacct gaacccgttc
     7261 atcggtcacc acaacggtga catcgtgggt aaaatctgtg ccgaaaccgg taagagtgta
     7321 cgtgaagtcg ttctggaacg cggtctgttg actgaagcgg aacttgacga tattttctcc
     7381 gtacagaatc tgatgcaccc ggcttacaaa gcaaaacgct atactgatga aagcgaacag
     7441 taatcgtaca gggtagtaca aataaaaaag gcacgtcaga tgacgtgcct tttttcttgt
     7501 gagcagtaac ttaaaaataa caatctaata tcaacttgtt aaaaaacaag gaaggctaat
     7561 atgctagttg tagagctcat catagttttg ctggcgatct tcttgggcgc cagattgggg
     7621 ggaataggta ttggttttgc aggcggattg ggggtgctgg ttcttgccgc tattggcgtt
     7681 aaacccggta acatcccgtt cgatgtcatt tccattatca tggcggttat cgccgctatt
     7741 tctgccatgc aggttgctgg cggtctggac tatctggttc atcagacaga aaagctgctg
     7801 cgccgtaacc cgaaatacat cacgatcctc gcaccgatcg tgacctattt cctgactatc
     7861 tttgctggta ctggcaacat ctctctggcg acactgccag ttatcgctga agttgcgaag
     7921 gaacaaggcg ttaaaccttg ccgtccgctc tctactgcag tggtatccgc gcagattgcg
     7981 atcaccgcat cgccaatctc agcggcagtg gtttacatgt cttccgtgat ggaaggtcat
     8041 ggcatcagct acctccatct gctctccgtg gtcatcccgt ccaccctgct ggcggttctg
     8101 gtgatgtcct tcctggtcac tatgctgttc aactccaaac tctctgacga tccgatttat
     8161 cgcaagcgtc tggaagaggg cttggttgaa ctgcgcggtg aaaagcagat tgaaatcaaa
     8221 tccggtgcaa aaacgtccgt ctggctgttc ctgctgggcg tagttggcgt ggttatctat
     8281 gcaatcatca acagcccaag catgggtctg gttgaaaaac cgctgatgaa caccaccaac
     8341 gcaatcctga tcatcatgct cagcgttgca actctgacca ccgttatctg taaagtcgat
     8401 accgacaaca tcctcaactc cagcaccttc aaagcaggta tgagcgcctg tatttgtatc
     8461 ctgggtgttg cgtggctggg cgatactttc gtttccaaca acatcgactg gatcaaagat
     8521 accgctggtg aagtgattca gggtcatccg tggctgctgg ccgtcatctt cttctttgct
     8581 tctgctctgc tgtactctca ggctgcaacc gcaaaagcac tgatgccgat ggctctggca
     8641 ctgaacgttt caccgctgac cgctgttgct tctttcgctg cggtgtctgg tctgttcatt
     8701 ctgccgacct acccgacgct ggttgctgcg gtacagatgg atgacacggg tactacccgt
     8761 atcggtaaat tcgtcttcaa ccatccgttc ttcatcccgg gtactctggg tgttgccctg
     8821 gccgtttgct tcggcttcgt gctgggtagc ttcatgctgt aatgatcaat cgcggggcgt
     8881 tcacgccccg ctttctttcc cgccgactaa catccttccc ccgtccgttg tatagtgacc
     8941 tctctcttgc ggttccatct gttcttgcga ggtgtttatg cttgatgaaa aaagttcgaa
     9001 taccacgtct gtcgtggtgc tatgtaccgc accggatgaa gcgacagccc aggatttagc
     9061 cgccaaagtg ctggcggaaa aactggcggc ctgcgcgacc ttgatccccg gcgctacctc
     9121 tctctattac tgggaaggta agctggagca agaatacgaa gtgcagatga ttttaaaaac
     9181 taccgtatct caccagcagg cactgctgga atgcctgaag tctcatcatc catatcaaac
     9241 cccggaactt ctggttttac ctgttacaca cggagacaca gattacctct catggctcaa
     9301 cgcatcttta cgctgattct gctactttgc agcacttccg tttttgccgg attattcgac
     9361 gcgccgggac gttcacaatt tgtccccgcg gatcaagcct ttgcttttga ttttcagcaa
     9421 aaccaacatg accttaatct gacctggcag atcaaagacg gttactacct ctaccgtaaa
     9481 cagatccgca ttacgccgga acacgcgaaa attgccgacg agcagctgcc gcaaggcgtc
     9541 tggcatgaag atgagtttta cggcaaaagc gagatttacc gcgatcggct gacgcttccc
     9601 gtaaccatca accaggcgag tgcgggagcg acgttaactg tcacctacca gggctgtgct
     9661 gatgccggtt tctgttatcc gccagaaacc aaaaccgttc cgttaagcga agtggtcgcc
     9721 aacaacgcag cgtcacagcc tgtgtctgtt tcgcagcaag agcagcacac cgcgcaattg
     9781 cccttttccg cgctctgggc gttgttgatc ggtattggta tcgcctttac gccatgcgtg
     9841 ctgccaatgt acccactgat ttctggcatc gtgctgggtg gtaaacagcg gctctccact
     9901 gccagagcat tgttgctgac ctttatttat gtgcagggga tggcactgac ttacacggcg
     9961 ctgggtctgg tagttgccgc cgcagggtta cagttccagg cggcgctaca gcacccatac
    10021 gtgctcattg gcctcgccat cgtctttacc ttgctggcga tgtcaatgtt tggcttgttt
    10081 actctacaac tcccctcttc gctgcaaaca cgtctcacgt tgatgagcaa tcgccaacag
    10141 ggcggctcac ctagcggtgt gtttgttatg ggggcgattg ccggactgat ctgttcacca
    10201 tgcaccaccg caccgcttag cgcgattctg ctgtatatcg cccaaagcgg gaacatgtgg
    10261 ccgggcggcg gcacgcttta tctctatgcg ttgggcatgg gcctgccgct gatgctaatt
    10321 accgtctttg gtaaccgctt gctgccgaaa agcggcccgt ggatggaaca agtcaaaacc
    10381 gcgtttggtt ttgtgatcct cgcactgccg gtcttcctgc tggagcgagt gattggtgat
    10441 gtatggggat tacgcttgtg gtcggcgctg ggtgtcgcat tctttggctg ggcctttatc
    10501 accagcctac aggctaaacg cggctggatg cgcgtggtgc aaataatcct gctggcagcg
    10561 gcattggtta gcgtgcgccc acttcaggat tgggcatttg gtgcgacgca taccgcgcaa
    10621 actcaaacgc atctcaactt tacacaaatc aaaacggtag atgagttaaa tcaggcgctc
    10681 gttgaagcca aaggcaaacc ggtgatgtta gatctttatg ccgactggtg cgtcgcctgt
    10741 aaagagtttg agaaatacac cttcagcgac ccgcaggtgc aaaaagcgtt agcagacacg
    10801 gtcttacttc aggccaacgt cacggccaac gacgcacaag atgtggcgct gttaaagcat
    10861 cttaatgtcc ttggcctacc gacaattctc ttttttgacg gacaaggcca ggagcatcca
    10921 caagcacgcg tcacgggctt tatggatgct gaaaccttca gcgcacattt gcgcgatcgc
    10981 caaccgtgaa cgacacttgc agtgggaaag acggaggaaa atagcgtgca acgtgaagat
    11041 gtactgggag aagccctgaa attattagaa ttacaaggga ttgccaacac cacgctggag
    11101 atggttgctg aacgtgtgga ttatccactg gacgagctac gccgcttctg gccagacaaa
    11161 gaggcaatcc tctacgatgc gctgcgttat cttagccaac agatagatgt ctggcgtcgt
    11221 cagctgatgc tggacgaaac gcaaaccgcc gaacaaaagc tgctggcacg ttatcaggcg
    11281 ttatcggagt gcgttaaaaa caaccgctat ccgggctgtc tatttatcgc tgcctgtacg
    11341 ttttatcccg atcctggcca ccctattcat caactggccg atcagcaaaa aagcgctgcc
    11401 tacgatttca cccacgaact gttaaccacg ctggaagttg acgatccggc gatggtagca
    11461 aagcagatgg aactggtgct ggaaggctgt ttaagccgaa tgctggtgaa tcgtagccag
    11521 gcggatgtcg acactgcaca tcggctggcg gaagatatcc tgcgcttcgc ccgctgccgc
    11581 cagggtggtg cattgacctg acagaaacac agaaaagaag cgatttgccg caatcttaag
    11641 cagttgaatc gcttttactg aaattaggtt gacgagatgt gcagatta
//
LOCUS       DAJVQR010000112        11666 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVQR010000112 DAJVQR010000000
VERSION     DAJVQR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..330
                     /locus_tag="OQM52_003371"
     CDS             <1..330
                     /locus_tag="OQM52_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1565094.1"
                     /translation="LDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRK
                     PAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDLLLR
                     ATGGVCV"
     gene            complement(447..722)
                     /gene="yjfN"
                     /locus_tag="OQM52_003372"
     CDS             complement(447..722)
                     /gene="yjfN"
                     /locus_tag="OQM52_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1565095.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(871..1200)
                     /gene="bsmA"
                     /locus_tag="OQM52_003373"
     CDS             complement(871..1200)
                     /gene="bsmA"
                     /locus_tag="OQM52_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1565096.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1382..2131
                     /gene="yjfP"
                     /locus_tag="OQM52_003374"
     CDS             1382..2131
                     /gene="yjfP"
                     /locus_tag="OQM52_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1565097.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2128..2883)
                     /gene="ulaR"
                     /locus_tag="OQM52_003375"
     CDS             complement(2128..2883)
                     /gene="ulaR"
                     /locus_tag="OQM52_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1565098.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2991..4055)
                     /gene="ulaG"
                     /locus_tag="OQM52_003376"
     CDS             complement(2991..4055)
                     /gene="ulaG"
                     /locus_tag="OQM52_003376"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1565099.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4410..5807
                     /gene="ulaA"
                     /locus_tag="OQM52_003377"
     CDS             4410..5807
                     /gene="ulaA"
                     /locus_tag="OQM52_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1565100.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5823..6128
                     /gene="ulaB"
                     /locus_tag="OQM52_003378"
     CDS             5823..6128
                     /gene="ulaB"
                     /locus_tag="OQM52_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1565101.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6138..6602
                     /gene="ulaC"
                     /locus_tag="OQM52_003379"
     CDS             6138..6602
                     /gene="ulaC"
                     /locus_tag="OQM52_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1565102.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6616..7266
                     /gene="ulaD"
                     /locus_tag="OQM52_003380"
     CDS             6616..7266
                     /gene="ulaD"
                     /locus_tag="OQM52_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1565103.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7276..8130
                     /gene="ulaE"
                     /locus_tag="OQM52_003381"
     CDS             7276..8130
                     /gene="ulaE"
                     /locus_tag="OQM52_003381"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1565104.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8130..8816
                     /gene="ulaF"
                     /locus_tag="OQM52_003382"
     CDS             8130..8816
                     /gene="ulaF"
                     /locus_tag="OQM52_003382"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1565105.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8945..9220)
                     /locus_tag="OQM52_003383"
     CDS             complement(8945..9220)
                     /locus_tag="OQM52_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1565106.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9547..9942
                     /gene="rpsF"
                     /locus_tag="OQM52_003384"
     CDS             9547..9942
                     /gene="rpsF"
                     /locus_tag="OQM52_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1565107.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9949..10263
                     /gene="priB"
                     /locus_tag="OQM52_003385"
     CDS             9949..10263
                     /gene="priB"
                     /locus_tag="OQM52_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1565108.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10268..10495
                     /gene="rpsR"
                     /locus_tag="OQM52_003386"
     CDS             10268..10495
                     /gene="rpsR"
                     /locus_tag="OQM52_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1565109.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10537..10986
                     /gene="rplI"
                     /locus_tag="OQM52_003387"
     CDS             10537..10986
                     /gene="rplI"
                     /locus_tag="OQM52_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1565110.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11057..11620)
                     /locus_tag="OQM52_003388"
                     /pseudo
     CDS             complement(11057..11620)
                     /locus_tag="OQM52_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2763 a         2868 c         3083 g         2952 t
ORIGIN      
        1 ctggatgtgt tgcgcgttct caataagcaa gcgggcgtat acgacttatt gtcggaagca
       61 tttgtggaag tgaaagggca ggatcgctat tttgatcgcg cggttcgtcg tttacagcag
      121 cagctgcgta agccagctga agaactgggg cgagagatta ctcatcagct attcctgctg
      181 ggctgcggtg cgcaaatgtt gaaatatgct tctccgccaa tggcgcaggc gtggtgtcag
      241 gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc agatccagaa tgatttattg
      301 ctgcgggcga cggggggagt gtgtgtgtaa gcgtatacga ctgatgcgac gctggtttcg
      361 attaactaaa tgaaatatgt gaaaattgta ggccggacaa ggcgctcgcg ccgcatccgg
      421 cattgttcat ttgctgtaga tgacactcat gcatacagta tcgcctgtac gcgccagtta
      481 tcaggctgct ggtcttcgta catggtaata atgcggtacc atgacgcacc ttgttcatcg
      541 gcctttaacg ccacgatacg ttccacgtcc tgcggatccc ctgaaatatt gctaactgag
      601 atctgaccta tttcattcag gcccgtcacg cagtcagcac tggcgaattc tgcagactgt
      661 gccgtggcgc tcgcaggata agtcactaat tgcagcgagg gtgaggcaag taattgtttc
      721 atcgtcagct ccatttttcg tttccccaca tgttgcaggg cattccatcg cggtaataaa
      781 cgcccaagct tccagccggt aatttattgt gcacaggtaa acgcataggg acgcaaagca
      841 atgtaaagct gactatgtaa gttgtctgat ttatttacgg tacaaaatgg cttgtgaata
      901 ccactgtcct gtcacgatgg tttcgtccac catgacgaca acgtaataat cagcttttgc
      961 agcgacagct tttgctctga tttctgctaa tgcgtcatcc ggagaacccc gaaccatcgt
     1021 gcttacgcta cctattcgct gtaacccttg cgtctggtcg cggcgaatct cttgcggatg
     1081 gtccgttact ggcggtgctg gctgtggcgt accttgcagt gcgctacagg cacttaacat
     1141 caacaccaat aataaactgg caaaccggta aataacgcta ttacgtttcc tgctaaccat
     1201 agtgtagtgc cttattaatt taactttggg ggaatgttcc cgaattgtta cctggtacgc
     1261 gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc tcaacattat gaactaatac
     1321 cgccagcaga aacttgtgct aaggatttct tcatgtcata ccagacaaaa aggagagaca
     1381 gatgattgaa atagaatcac gcgagctggc agatattccc gttcttcatg cttatcctgt
     1441 cgggcaaaaa gataccccgt taccgtgcgt aattttttat cacggcttta cttcatccag
     1501 cctggtgtat agctattttg ccgttgcgct ggcgcaggct ggtttgcggg tgatcatgcc
     1561 ggatgcgccc gatcacggta gccgttttag tggcgacgaa gcgcggcggt taaatcaatt
     1621 ctggcaaatc ttgctacaaa gtatgcagga attcactact ttacgtgcgg caatagccga
     1681 agaaaactgg ctgcttgatg accgtctggc agtcggtggc gcgtcgatgg gcgcgatgac
     1741 ggcactggga attaccgctc gccaccccac ggtgaaatgt accgccagca tgatgggatc
     1801 gggctatttt acatcgctcg cccgttcact gtttccaccg ctgatacctg aaacggcagc
     1861 acagcagaat gaattcaata aaattgtcgc gccgctggca gagtgggaag cgacaaacca
     1921 cctggaacaa cttggggaca gacctctact gctgtggcat ggcctcgacg atgacgttgt
     1981 gcctgccgac gaatcactac gtttgcagca ggccttaagc gagacgggac gggataaact
     2041 gctaacctgt tcatggcagc caggcgtgcg tcaccgcatt acgcctgagg cgttagatgc
     2101 tgccgtgaca tttttccgcc agcatcttta aacacgcaga atgctgaccc cttgcgcttc
     2161 cagttgttgc aggatttccg ggttagcatt tttgccggtg atcagcatat cgatttgatc
     2221 ggcacggcta aaaagcattc ccgcgcgttc gccaatctta ctgctatcaa ccagtaccac
     2281 cagtttccct accacgctca gcatcttctg ctctgccatt gctgtcagca tatcggtttt
     2341 atacaaccct tctgcggtca gcccttttcc gctggtaaac atccagtgcc cggcatagag
     2401 actgttttcg ctgccctgcg ggcttaaagt gatggactga cttttgttgt actgtccacc
     2461 cataatgatc acgctgtcat gttcttgatc gatcaggtaa tttgccagcg gtagataatt
     2521 agtgatgatt tgcactggct tgccacacat ttcccgccca agcagaaacg cggtggagcc
     2581 gcagttgatg actacgcttt cgcccggatt aaccagctgc gaggccgctt tagcaatacg
     2641 tactttttca tcgtgattct gcgcctgatg cagattcatc ggcgtccagc gcgggcgctg
     2701 ttgggtaata gcttctgcgc cattgcgcac ctttttcagt ttgccgcttt cgccaagttt
     2761 attgatatcg cgtcgcgcag tggcaggcga aattcccaga cgctcaacga ctttctcaac
     2821 ggtcacaaag cccaattgtg cgagcatttc caggaggatt tgatgtcttt gtgcttcagt
     2881 catgagctat tccgataaaa tttgattttt ttagatgata tttgaaatag ccaggaaata
     2941 ctacgccgga tagcgcgaac ttctccacgc tatccggtat aaacgacaga ttacaggaat
     3001 gacttgaacg gcagatccgg ttcaatagtg aagcaatcat cgaaaccgcg cggatagtgg
     3061 tactcgaagt tgtctttatc cagcggccag gtaaatttgc cgccaacctg ccagataaac
     3121 ggcttgaagc catacttcag acgatctttt ttgatctccc acagcacgcg gatctcttgc
     3181 ggatcggcct ggaagtttga ccagatgtcg tggtggaacg ggataaccac tttcgcattc
     3241 agggcttcac ccatacgcag catatcggcg ctggtcattt tgtcggtaat accgcgcggg
     3301 ttctcaccat acgatcctaa cgccacatcg atctgatgtt cgttaccgtg cttcgcataa
     3361 tagttagagt agtgggaatc gccactgtga tatagggaac cgccaggggt tttgaacagg
     3421 tagtttactg cgcgatcgtc catgccatct ggcagtacgc cagccgcttt ttgatcggca
     3481 ggcagggtga tcagtgcagt acggtcgaaa gcgtcaagcg catgaatttc aatgtctttc
     3541 acttttacaa catcgcccgg tttgaccacg atgcaacgct ctttcggtac gccccagcca
     3601 atccacaaat caacgcaggt tttcggtcca atgaatggta cgtcatcagc acagttctgc
     3661 atcacggcag cagcgacgtt aacgtcgata tgatcgttgt gatcgtgagt cgccagtacc
     3721 gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg gggtggtgcg taggtttggc
     3781 tgcagttttt tcacgccagc catgcgctgc atctggtgac cctgtttcat taacgggtta
     3841 ccgtgacttt gtttgccagt gccgcaccag aaatcaacgc aaacgttggt gccaccttct
     3901 gatttcaacc agatcccggt gcagcctagc caccacatcg caaatgtgcc aggggcgacc
     3961 tgctcttgtt caatttcttc attcagccag ctaccccact ccgggaaagt gctcaggatc
     4021 caggattctc gggtgatact tttcacttta ctcatcgcca tttaccttca tgatagttca
     4081 attcgaatca acatgtgatt ggttttgatt aatcctgaca ctattccttc aggaaggtaa
     4141 tgaccatttt ttgacttttt gccagggaag ttgttgttga ttttgagtgg ggaaagattt
     4201 aatggaatgt gtagttaaat taactaaatg aatttaaata aataattgtt tcgttgtgtg
     4261 aatcccactc tatccatgtg gaattatttg cgggtcgcgt cacatttaat cataaataat
     4321 cttgttgtga ttacttttga aaattagagt gagtgcacaa cattccgggt gtgcggaata
     4381 cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc tttaccgtgt
     4441 tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc tgtctgggct
     4501 acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa accataattg
     4561 gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa ccggtggtgg
     4621 cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc cgatacttac gcgtcgatga
     4681 tggcaaccat cgaccgcatg ggcgatgcct atagctgggt aggttacgcg gtgttgttag
     4741 cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt cgtaccatca
     4801 tgctgactgg tcatatcatg ttccagcagg cggggctgat tgccgttacg ttgtttattt
     4861 tcggctactc catgtggaca accattatct gcacggcgat tctggtttcg ctctactggg
     4921 gcattacctc caacatgatg tacaagccga ctcaggaagt gacggacggc tgcggtttct
     4981 ccatcggtca ccagcaacag tttgcatcat taattgccta taaagttgcg ccgttcctcg
     5041 gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg ctggctgaat attttccacg
     5101 acaacatcgt ctccacggcg attgtcatga ccatcttctt tggtgccatt ctgctctcct
     5161 tcggtatcga caccgtgcag gcgatggcag gcaaagtgca ctggacggtc tatatcctgc
     5221 aaaccggttt ttccttcgcg gtggcgatct tcatcatcac tcagggtgtg cgcatgtttg
     5281 tggcggaact ctctgaagcg tttaacggta tctcccagcg cctgatccct ggtgcggttc
     5341 tggcgattga ctgtgcggct atttatagct tcgctccgaa cgccgtggtc tggggcttta
     5401 tgtggggcac catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc ggttcctcga
     5461 tcctgattat cccaggcttt atcccgatgt tcttctccaa cgccaccatc ggcgtgttcg
     5521 ctaaccactt cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg gggatgatcg
     5581 aaatctttgg ttgcgtctgg gtggtgaaac ttaccggtat gagtgcctgg atggggatgg
     5641 cggactggtc gattctggca ccgccgatga tgcagggctt cttctccatc ggtatcgcct
     5701 ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc cgcgcactgc
     5761 gcgcagaaga agatgcagaa aaacaattgg cagaacagtc tgcttaataa ggagttttga
     5821 ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc atgatcatga
     5881 agatgaaagt ggaccagttt ttaacccaat caaacattga ccacacggta aatagctgcg
     5941 cggttggcga atacaaaagc gagttgagcg gcgcggatat catcatcgct tctacgcaca
     6001 ttgcgggcga aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc aacatgctct
     6061 ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agcgcatttc ccgcaggatg
     6121 tgaagtaagg acgcgccatg aaattacatg attcgctggc ggagaataaa tccatccgcc
     6181 tgcaggctga agctgagaca tggcaggacg cagtaaaaat cggcgttgac ctgctggtgg
     6241 cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt gaacagcacg
     6301 gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg gaagagggcg
     6361 ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc aaccacgaag
     6421 ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac acgcaccagg
     6481 aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc gaccgtttac
     6541 gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac gcggcagctt
     6601 aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa ccagactatg
     6661 gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat cgaagtgggt
     6721 accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc gctctacccg
     6781 cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct ttctcgtatg
     6841 tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat caacaccgcc
     6901 aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga actgaccggt
     6961 tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca ggtggtttat
     7021 caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga tatcactgcg
     7081 atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct ggcgctggaa
     7141 gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg cagcatccgt
     7201 gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc cgaactgtgg
     7261 ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga aaaagcgctc
     7321 cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg cttcgatttt
     7381 gtcgagatgt cggtagatga aactaacgag cgcctgtcgc gccttgactg gagccgcgaa
     7441 cagcgtctgg cgctggtgaa tgctattgtt gaaaccggcg tgcgtgtgcc gtccatgtgc
     7501 ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg ggcgcagggg
     7561 ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg cgtgatccag
     7621 ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg tcgtttccgt
     7681 gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct ggcgatggag
     7741 atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc gcactatctc
     7801 aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg ggacaacgat
     7861 gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt gaaagacacc
     7921 aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt cgaacgttgc
     7981 ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat gtggagcgaa
     8041 acggcggaag acccggcggc agaagtggtg aaagcgtgtg attgggtgaa agcgcgcatg
     8101 gccaaagcag gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtgtttgaag
     8161 ccaacatgga tctgccgcgc tatgggctgg tgacctttac ttggggcaac gtcagcgcta
     8221 tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga
     8281 aagcagacga tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg gagtatcgcc
     8341 catcttccga cactgcgacg catctcgaac tctaccgtcg ttacccgtcg cttggcggca
     8401 ttgtccatac ccactccact catgccaccg catgggcgca ggcggggctg gcgatcccgg
     8461 cattaggcac cacgcacgct gactacttct ttggtgatat cccgtgtacg cgtggattaa
     8521 gcgaagaaga ggtgcagggt gagtatgaac tgaacactgg caaagtgatt atcgaaacgc
     8581 tgggcaacgc cgagccgctg catacgccgg gaattgtggt gtatcagcac ggaccgttcg
     8641 cctgggggaa agatgcccac gacgcggtgc ataacgcggt agtgatggaa gaagtggcga
     8701 aaatggcgtg gattgcgcgc agtattaatc cacaactcaa tcatatcgac agctacctga
     8761 tgaataaaca cttcatgcgt aagcacggtc ctaacgctta ttacgggcag aagtaaaaca
     8821 cgcgctgcgg aaatttcctt cctcgggaga taactggtct aattccgcag ccgtttttca
     8881 aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct
     8941 ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca
     9001 tcagaattac gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt aatgcctgac
     9061 gtgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat
     9121 cgtccatatt tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta
     9181 gcaaaagcac agccagaagg gctaaaactc gactgaacat agatacctcc tcgacggctg
     9241 actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag
     9301 ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga atgtgatgaa
     9361 cttcaaatca gcggtttaga ggttatttgc gaaaggggag atttatttct gctccgccct
     9421 tgagtttagc ggggtatgcc agtactataa cgcgtcattt ttcagccgac ctttaacacg
     9481 ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc
     9541 aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt
     9601 ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt
     9661 ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac
     9721 tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga aactaccttc
     9781 cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa
     9841 gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac
     9901 gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt
     9961 ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt
    10021 cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ctttcaccgg
    10081 caggcatggt gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac
    10141 agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag
    10201 aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga ttctggagac
    10261 tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt
    10321 tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa
    10381 gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc
    10441 tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt
    10501 cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa
    10561 gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac
    10621 ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa
    10681 gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc
    10741 gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt
    10801 aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt
    10861 gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac
    10921 gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct
    10981 gaataattcg ttattcaacg agacgtaaaa acgccgacca ttggtcggcg ttttgctttc
    11041 tatttttcgt caggcattaa tttcgcaagt agatccattc accattctct tgtgtccata
    11101 agtcaggatg gtttgctggc gttagatgca tacgatcact ccttatgtct cggtcctgcg
    11161 gcactcgctc gaaacctaaa cccgagtaga ttgcggctga atttgataag gaacgtagac
    11221 caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc
    11281 cgagggcatt agaaacgcct gtaacagtgt gaagttggtc gatataaaga tcgattgttg
    11341 gtagttcaga tgagcagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg
    11401 tttggccggt atagtctgga gtactataac caggtcccaa aacaacctcg tgtattgtgt
    11461 atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta
    11521 acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg aatattatct aatgtagctt
    11581 catggttcca tccaaaagaa ttatcaccat tcattgttac tgaatcgcca cgggtttaac
    11641 agacacctca gagtcattta agatgg
//
LOCUS       DAJVQR010000113        11657 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVQR010000113 DAJVQR010000000
VERSION     DAJVQR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            597..794
                     /locus_tag="OQM52_003389"
     CDS             597..794
                     /locus_tag="OQM52_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565111.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OQM52_003390"
     CDS             complement(744..1937)
                     /locus_tag="OQM52_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1565112.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OQM52_003391"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OQM52_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1565113.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OQM52_003392"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OQM52_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1565114.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OQM52_003393"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OQM52_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1565115.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OQM52_003394"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OQM52_003394"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1565116.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OQM52_003395"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OQM52_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1565117.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OQM52_003396"
     CDS             complement(10573..10716)
                     /locus_tag="OQM52_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565118.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11657
                     /locus_tag="OQM52_003397"
     CDS             10955..>11657
                     /locus_tag="OQM52_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1565119.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT         2956 a         2898 c         2992 g         2811 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggact
//
LOCUS       DAJVQR010000114        11499 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVQR010000114 DAJVQR010000000
VERSION     DAJVQR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(205..280)
                     /locus_tag="OQM52_003398"
     tRNA            complement(205..280)
                     /locus_tag="OQM52_003398"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Ile,seq:cat)
     gene            839..3091
                     /locus_tag="OQM52_003399"
     CDS             839..3091
                     /locus_tag="OQM52_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565120.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRSLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3427..4359
                     /gene="csiD"
                     /locus_tag="OQM52_003400"
     CDS             3427..4359
                     /gene="csiD"
                     /locus_tag="OQM52_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1565121.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4379..5647
                     /gene="lhgO"
                     /locus_tag="OQM52_003401"
     CDS             4379..5647
                     /gene="lhgO"
                     /locus_tag="OQM52_003401"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1565122.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5670..7118
                     /gene="gabD"
                     /locus_tag="OQM52_003402"
                     /pseudo
     CDS             5670..7118
                     /gene="gabD"
                     /locus_tag="OQM52_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7126..8412
                     /gene="gabT"
                     /locus_tag="OQM52_003403"
                     /pseudo
     CDS             7126..8412
                     /gene="gabT"
                     /locus_tag="OQM52_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8650..10050
                     /gene="gabP"
                     /locus_tag="OQM52_003404"
     CDS             8650..10050
                     /gene="gabP"
                     /locus_tag="OQM52_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1565123.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10071..10733
                     /gene="csiR"
                     /locus_tag="OQM52_003405"
     CDS             10071..10733
                     /gene="csiR"
                     /locus_tag="OQM52_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1565124.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10734..11183)
                     /gene="kbp"
                     /locus_tag="OQM52_003406"
     CDS             complement(10734..11183)
                     /gene="kbp"
                     /locus_tag="OQM52_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1565125.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11267..11425)
                     /locus_tag="OQM52_003407"
     CDS             complement(11267..11425)
                     /locus_tag="OQM52_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1565126.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2961 a         2822 c         2867 g         2849 t
ORIGIN      
        1 gcatagcggg cattttttcc aggtactttt gaatgagtac tgatgaataa atacattgca
       61 gtggcgtgcc acgtaccaaa acaccagccc tcattcgaaa ccacccaccg cacttcttcc
      121 ttgaaatggc gttagtcatg aaatatagac cgccatcgag taccccttgt acccttaact
      181 cttcctgata catgaatgat gatttggtgg cccttgctgg acttgaacca gcgaccaagc
      241 gattatgagt cgcctgctct aaccactgag ctaaagggcc ttaagtatgc aataacaata
      301 cttataaacc acgcaataaa catgatgatc ttatgatgta aataacagat ttttatgcgt
      361 tcccattcac cttgggtcgt gattacacgc gacataaaac ccgacaccgc ctccattcgc
      421 aaagtcgata ctcgcagtca acaagcaaat gttaataatt agcactatct atagttatca
      481 tcgattcaat gatactttgt aatgattttg tatctaataa tataacttta ttacattagc
      541 tgaagagttt tcgcatcatt atgatatctg ttacttttca ctccataaaa ataaacttca
      601 tatagcaata tattctttca tagatcttat tctgctaatc attagtttcg tatgcgcgat
      661 ttttgacagt tgcctctcca gaccacatcg ataattaaaa aacagattta aacattatcc
      721 ttttccatat agatattgga taaaaatagg acatctgttt gcaattactt tcacaacaat
      781 taaacatttt tatgttttcg tatacatcat attactctac cattagagga acgttattat
      841 gttttctata aaaccaggac ctggaaattt acctatcgac aaccccacat tgttatcatg
      901 gaacattact gatggggatc taaattccaa atttaataca ttagaatatc taaactgtat
      961 aacaaatatt attaatgctt gtggagttta tcctcaggat ttaaaagaca gagaaattat
     1021 atcaactttt cacgcagaaa aagtcattaa tgatctgtta aaaaacgatt ataaaatttc
     1081 cctttctcca gatacaactt accgagagct gaataaagct gcacagagta gcattacagc
     1141 gccagacagg ataggagaag ggaaaacatg ggtttatcaa cgagatacaa tggttgaaag
     1201 aggtgataac agtggtgttc atcagtatgg tccagctgaa catttcactc acattatatc
     1261 tgacaaaccg tccccaaaag ataagtatgt tgcatatgct attaacattc ctgactatga
     1321 gctagcagcc gatgtatata atattaacgt gacgtcacct tccggacagc aagaaacatt
     1381 taaaatacta atcaatccag aacatctacg gcaaacactt gagcgtaaat ctcttactgc
     1441 tgttcagaaa tcacaatgtg aaatcatcac ccccaaaaaa cctggcgaag cgattcttca
     1501 tgcttttaat gccacctacc agcaaatcag ggaaaatatg tctgagtttg cacgttccca
     1561 ttatgggtat atacaaatcc ctccagtgac aactttccgc gccgacggac cagaaactcc
     1621 cgaagaagaa aaaggttact ggtttcacgc ttatcaaccc gaagatcttt gtaccatcca
     1681 taatccaatg ggggatttgc aggattttat cgcattggtt aaagatgcta aaaaatttgg
     1741 tatcgatatc attcctgatt atacctttaa ctttatggga atcgggggaa gtggtaaaaa
     1801 tgacctggat tatccctctg ctgatatacg agcgaagatc agtaaagata tagaaagtgg
     1861 tatccctggc tattggcaag gtcaggtttt gattccattc attaaagatc cagtaacaaa
     1921 agaacgtaaa caaatccatc cagaagatat acatctcact gcaaaagact tcgaagcaag
     1981 taaagataac atctctaagg atgaatggaa aaacctccat gcattaaaag aaaagagttt
     2041 aaatggaatg cctaaaacaa cccccaaaag tgaccaggtt attatgttgc aaaatcaata
     2101 cgttcgtgaa atgcgaaaat atggcgtacg aagtttacgt tatgatgcag caaaacactc
     2161 aaaacatgaa caaatagaaa gatcaataac cccaccgctt aaaaattata atgagcgatt
     2221 acacaatact aacttattta acccaaaata tcatgaaaaa accgttatga attacatgga
     2281 atatctggta acttgtcagt tgaatgaaga acaaatgtca tcgctgcttt atgaaagaga
     2341 tgatttaagc gccattgatt tttcattgct catgaagacg ataaaagcct tttcattcgg
     2401 gggagatctc ctaacccttg catcaaaacc gggttccaca atctcaagca tcccatcaga
     2461 aagacggata ttgattaaca ttaaccacga ttttcctaac aatggcaatc ttttcaatga
     2521 ctttctattt aaccatcaac aagatgaaca attagcaatg gcatatatgg ccgctctccc
     2581 gttcagcagg cctttagttt actgggatgg ccaggtatta aaatcaacga ctgaaattaa
     2641 aaattatgat gggcccacgc gtgtcggtgg tgaggcgtgg cttaataaag gttgctctac
     2701 ctatcagcag ctctacaatg aattccacgc attatatata gataaagcag gaatatggag
     2761 cgcatttgag ggtgtatttg caactaaaaa cgttctggcc tttagtcgtg gggattctgt
     2821 gaacattaat cactctcctc atgatggact agttataata aataaaggaa acgaagaagt
     2881 tgaaggtacc tggcctaaca aattgcaacc tggaatatat aaaaacatgg ggagtaatag
     2941 cgttaacatt attattaata atacccgaaa aattatcccc ccttgtaaag tatttacgct
     3001 tagaggcgga tctctaaata tcaatattcc taggcgtagc gctcttcttt tagggaaaac
     3061 aggagaaccg ccgaactatc tctatttata atttaatgtt atatctgccc cgataaaacg
     3121 gggcagataa tatgtttagt ttactaacgg tcattttgta gtgaagccat ttactgtttt
     3181 ttatcgacca gataatctgt tctctaatgt taactccccc taacctgttg ctttagttat
     3241 tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat ttgttgcttt
     3301 tgatcacaat aagaaagcaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt
     3361 tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg
     3421 cttctgatga atgcactgac cgccgtacaa aataacgctg tcgattcagg ccaggactat
     3481 agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc
     3541 gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca ggcgctggag
     3601 tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag
     3661 ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat
     3721 gcggtaggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta
     3781 gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc
     3841 gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa
     3901 ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc
     3961 gatgactggg aacatctgga ccactatttc cgccatccac tggcgcgtcg cccgatgcgc
     4021 tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt tccatccagt gttcgacgtt
     4081 gatcaacagg gtcgcccggt gatgcgctat atcgaccagt tcgtccagcc aaaagatttt
     4141 gaagaaggcg tgtggttgag cgagctttcc gatgccattg aaaccagcaa aggcattctt
     4201 tctgtacccg ttcccgttgg caaattcctg ttgataaaca acctgttctg gctgcacggt
     4261 cgcgaccgct ttactccgca cccggatctg cgccgtgaac tgatgcgtca gcgtggctat
     4321 ttcgcttacg ccactcacca ctaccagacg catcagtaag cgcaaaggaa ttgagcggat
     4381 gtatgatttt gtgattattg gcggcggcat catcggcatg tcgaccgcca tgcaactgat
     4441 tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa gagtccggcc cggcctgtca
     4501 ccagacgggc cacaacagcg gcgtgatcca tgccggggtc tattacacgc ccggcagcct
     4561 gaaggcgcag ttttgcctgg cggggaaccg tgccaccaaa gccttttgcg atcaaaacgg
     4621 cattcgctat gacaactgcg gcaagatgct ggtcgccacg tctcagctcg aaatggaacg
     4681 gatgcgcgcc ttatgggaac gcacagcggc gaacggtatc gagcgcgagt ggataaacgc
     4741 cgtggaactg cgcgagcgcg aaccgaatat caccgggctt ggtggcattt ttgtgccgtc
     4801 cagcggcatt gtcagctacc gcgaagtgac ggcggcaatg gcaaaaatct tccaggccag
     4861 aggcggcgag attatttata acgccgaagt cagcgccctc agtgagcata aaaacggcgt
     4921 ggtgatacgc acccgtcagg gcggtgaata tgaagcatcg acgctgatta gctgttccgg
     4981 gctgatggct gaccggctgg tgaaaatgct cggcctcgaa ccgggcttta ttatctgccc
     5041 gttccgtggc gagtatttcc gccttgcgcc ggagcataac cagattgtta accacctgat
     5101 ttaccccatt cccgaccccg cgatgccatt tttgggcgtg catctcaccc gcatgatcga
     5161 cggcagcgtg accgtcgggc caaacgcggt gctggctttc aaacgcgaag gctatcgcaa
     5221 gcgcgacttc tcatttagtg acacgctgga gattttgagc tcgtcgggga ttcgccgggt
     5281 gctgcaaaac catctacgct caggactggg cgagatgaaa aactcgctgt gcaaaagcgg
     5341 ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg ttaagcgatc tccagccctg
     5401 gcccgccggt gtgcgggcgc aggcagtatc gccggacggc aagctgattg acgattttct
     5461 gtttgtcacc accccacgca cgatccacac ctgcaatgcg ccctccccgg cagcgacatc
     5521 agcaattcct attggtgcgc atattgtcag caaggtacaa acgctgttgg caagccagag
     5581 taaccccgga cgcacgctgc gagcggcacg tagtgtggat gccttacacg ccgcatttaa
     5641 tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa cgacagtaac ttattccgcc
     5701 agcaggcgtt gattaacggg gaatggctgg acgccaacaa tggtgaagtc atcgacgtca
     5761 ccaatccggc gaacggcgac aagctgggta gcgtgccgaa aatgggcgca gatgaaaccc
     5821 gcgccgctat cgacgccgcc aaccgcgccc tgcccgcctg gcgtgcgctc accgccaaag
     5881 aacgcgccac cattctgcgc aactggttca atttgatgat gcagcatcag gacgatttag
     5941 cgcgcctgat gaccctcgaa cagggtaaac cgctggctga agcgaaaggc gaaatcagct
     6001 acgccgcctc cttcattgag tggtttgctg aagaaggcaa acgtatttat ggcgacacca
     6061 ttcctggtca tcaggccgat aagcgcctga ttgttatcaa gcagccgatt ggcgtcaccg
     6121 ccgccatcac gccgtggaac tttccggcgg cgatgattac ccgtaaagcc ggtccggcgc
     6181 tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca gacgccgttc tctgcgctgg
     6241 cgctggcgga actggcgatt cgcgcgggca ttccggctgg cgtgtttaac gtggtcaccg
     6301 gttcggcagg cgtggtcggt aatgaactga ccagcaaccc gctggtacgc aaactgtcgt
     6361 ttaccggtta gaccgagatt ggccgccagt taatggaaca gtgcgcgaaa gacatcaaaa
     6421 aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt ctttgacgat gccgatctcg
     6481 ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa cgccgggcaa acctgcgtct
     6541 gcgccaaccg tttatacgtg caggacggcg tgtatgaccg ctttgccgaa aaattacagc
     6601 aggcggggag caaactgcac atcggcgacg ggctggagaa aggcgtcacc atcgggccgc
     6661 tgatcgatga aaaagcggta gcaaaagtgg aagagcatat tgccgatgcg ctggagaaag
     6721 gtgcgcgtgt ggtttgcggc ggtaaagcgc acgaacgcgg cggcaacttc ttccagccaa
     6781 ccattctggt ggacgttccg gccaacgcca aagtgtcgaa agaagagacg ttcggccccc
     6841 tcgccccact gttccgtttt aaagatgaag ccgatgtgat tgcacaagcc aatgacaccg
     6901 agtttggtct tgccgcctat ttctacgccc gtgatttaag ccgcgtcttc cgcgtcggcg
     6961 aggcgctgga gtacggcatc gtcggcatca ataccggcat tatttccaat gaagtggccc
     7021 cattcggcgg catcaaagcc tcgggtctgg gtcgtgaagg ttcgaagtat ggcatcgaag
     7081 attacttaga aatcaaatat atgtgcatcg gtctttaact ggagaatgcg aaggaacagc
     7141 aataaagagt taatgcagcg ccgcagtcag gcgattcccc gtggcgttgg gcaaattcac
     7201 ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg acgtttaagg ccgtgagtat
     7261 cttgatttcg cgggcgggat tgcggtgctc aataccgggc acctgcatcc gaaggtggtg
     7321 gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct gcttccaggt gctggcttac
     7381 gagccgtatc tggagctgtg cgagattatg aatcagaagg tgccgggcga tttcgccaag
     7441 aaaacgctgc tggttacgac cggttccgaa gcggtggaaa acgcggtaaa aatcgcccgc
     7501 gccgccacca aacgtagcgg caccatcgct tttagcggcg agtatcacgg gcgcacgcat
     7561 tacacgctgg cgctgaccgg caaggtgaat ccgtactctg cgggcatggg gctgatgccg
     7621 ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg gcataagcga ggatgacgct
     7681 atcgccagca tccaccggat cttcaaaaat gatgccgcgc cggaagatat cgccgccatc
     7741 atgattgagc cggttcaggg cgaaggcggt ttctacgccg cgtcgccagc ctttatgcag
     7801 cgtttacgcg ctctgtgtga cgagcacggg atcatgctga ttgccgatga agtgcagagc
     7861 ggcgcggggc gtaccggcac gctgtttgcg atggagcaga tgggcgtggc gccggatctt
     7921 accacctttg cgaaatcgat cgcaggcggt ttcccgctgg cgggcgtcac cgggcgcgcg
     7981 gaagtgatgg acgctgttgc accgggcggt ctgggcggca cctatgccgg taacccgatt
     8041 gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc aggaaaatct gctgcaaaaa
     8101 gccaacgatc tggggcagaa gctaaaagac ggattgttgg cgatagccga aaaacacccg
     8161 gagatcggcg acgtacgcgg gctgggatcg atgattgcca tcgagctgtt tgaagatggc
     8221 gatcacagca aaccggacgc aaaactcacc gccgagatcg tggcacgcgc ccgcgataag
     8281 ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct tgtaccactc
     8341 accattgaag acgctcagat ccgtcagggt ctggagatta tcggtcagtg ttttgatgag
     8401 gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg cgcgtcttgt ccggcctacg
     8461 agaatccaag tcggataagg cgtttacgcc gcatccgaca atctgtacgt gaacaggaat
     8521 aaatctatgt cggccggata aggcggagcc gctctccggc aaaaagaatc aataacaatt
     8581 aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga
     8641 ggattcagga tggggcaatc atcgcaacca cacgagttag gcggcgggct gaaatcacgc
     8701 cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc
     8761 gtcgccatcg ccgaagcgga cccggcggta ttactggcct atctgttcgc cggattactg
     8821 gtggttatga ttatgcggat gctggcggaa atggcagttg ccacgcccga taccggttcg
     8881 ttttccacct atgccgataa agccattggc cgctgggcgg gctataccat cggctggttg
     8941 tactggtggt tttgggtact ggttatccca ctggaagcca acatcgccgc catgatcctg
     9001 cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac cctcgcctta
     9061 actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg
     9121 tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt
     9181 tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg ctttatgccc
     9241 aacggtttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca
     9301 gaaattgtca ccattgccgc cgcggaatcc gacatgccgg aaaaacatat tgtccgcgcc
     9361 accaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtcgtg
     9421 gcattaatac cgtggaatat gccggggctg aaagccgttg gatcttatcg ctcggttctg
     9481 gaattgctca atattcccca tgcgaaatta atcatggact gtgtgatatt actttccgta
     9541 accagttgcc tgaactcggc cctgtatacc gcctcaagga tgctctactc cttaagccgt
     9601 cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg
     9661 gtgttactct ccaccggcgc agcattctta acggtggtgg tgaactatta cgcgcctgcg
     9721 aaggtattta aatttctgat cgacagctcc ggcgctatcg ccctgctggt ttatttagtc
     9781 atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc
     9841 ttgcgaatgt ggcttgatcc ctggctcacc tggctggtca tcggctttat tacctttgtg
     9901 ttggtagtga tgctattccg tccggcgcaa cagttagaag tgatctccac cggcttatta
     9961 gcgataggga ttatctgtac cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg
    10021 caaaaaacac ccgttcataa tacgcgctga taatgatcag gagtcacacc atgaccatta
    10081 cgtctctgga tggctatcgc tggctgaaga acgatattat tcgcggtaat tttcaaccgg
    10141 atgaaaaatt acggatgagt ttactgacat cgcgttatgc acttggcgtt gggccgttgc
    10201 aggaagcact ttctcaactg gtggcggaac ggctggtcac ggtggtgaat caaaaagggt
    10261 atcgggtggc gtctatgtca gagcaggagc tgctcgatat tttcgacgcc cgcgccaata
    10321 tggaagcgat gctggtcagt ctggcgattg cccgcggtgg cgatgaatgg gaggcagacg
    10381 ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg tgacgccagc gagaaaatgc
    10441 ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc aattgtggcg ggctgtggtt
    10501 ctcactattt gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgt tatcgattta
    10561 tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga ggataaacac gatcagcacc
    10621 agaccctgac tgcggcggta ctgccgcgag atacagcgcg cgccagtgag ttaatgcgcc
    10681 agcatttact gacgccaatt cccattatcc agcaggcgat ggctggcaat taactactct
    10741 tccggaatac gcaacacttg ccccggataa attttatccg ggctttttag catcggttta
    10801 ttcgcttcga agattttatt gtacagatta gcgttaccgt agacctgttt ggaaatggca
    10861 ctcagagtgt cgccagactt aacggtataa aactggctgg cagtggctgg tgtcgccgtt
    10921 ttcacctgat catcaacact ggcaataccg gaaatattcc ccaccgcaac aaggattttc
    10981 tccttcgcct cctgactcag gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga
    11041 atgttcactt tatcggcatc aggtataccg gttttgctca gatgctcctg caccttcttc
    11101 gcctgatcgt ctttatcgtg ctgacctgta accgcatccc agagtttttc tccggcatct
    11161 ttaacaaaat tgaacagacc catagctacc tcattcgtta acggaaacac tcagaaagtg
    11221 tagcagagga acgcggcgcg ttaacgccgc gggggtgagc acaggtttaa tcgcgggttt
    11281 gcacccagaa cgcgtgaatc aaaccgggga tatacccaag cagtgtcagc agaatattaa
    11341 taatgaacgc ccaaccgaac cctttaccga gcagcacgcc gatcggcggc agaatgatgg
    11401 tgatgacgat tctccagaaa cccatatgta ctccctataa gaaaattact cattgtttaa
    11461 aaagagattt tatctcttaa gcgtagtaat tttagcgga
//
LOCUS       DAJVQR010000115        11351 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVQR010000115 DAJVQR010000000
VERSION     DAJVQR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OQM52_003408"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OQM52_003408"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCS1565127.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="OQM52_003409"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="OQM52_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1565128.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="OQM52_003410"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="OQM52_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1565129.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="OQM52_003411"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="OQM52_003411"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1565130.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="OQM52_003412"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="OQM52_003412"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1565131.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="OQM52_003413"
     CDS             complement(5839..6879)
                     /locus_tag="OQM52_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1565132.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="OQM52_003414"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="OQM52_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1565133.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="OQM52_003415"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="OQM52_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1565134.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="OQM52_003416"
     CDS             7821..8261
                     /locus_tag="OQM52_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1565135.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="OQM52_003417"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="OQM52_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1565136.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="OQM52_003418"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="OQM52_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1565137.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11351
                     /gene="rsxC"
                     /locus_tag="OQM52_003419"
     CDS             9490..>11351
                     /gene="rsxC"
                     /locus_tag="OQM52_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1565138.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2651 a         2927 c         2865 g         2908 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgctccaaa gcgcgcaagc tggaacagca acaggctaat
    11221 gcggaaccag aagaacaggt cgatccgcgc aaagccgccg tcgaagccgc tattgcccgt
    11281 gccaaagcgc gcaagctgga acagcaacag gctaatgcgg aaccagaaga acaggtcgat
    11341 ccgcgcaaag c
//
LOCUS       DAJVQR010000116        11039 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVQR010000116 DAJVQR010000000
VERSION     DAJVQR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..260)
                     /locus_tag="OQM52_003420"
     CDS             complement(<1..260)
                     /locus_tag="OQM52_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1565139.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNN"
     gene            complement(354..916)
                     /locus_tag="OQM52_003421"
                     /pseudo
     CDS             complement(354..916)
                     /locus_tag="OQM52_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1120..1326)
                     /locus_tag="OQM52_003422"
     CDS             complement(1120..1326)
                     /locus_tag="OQM52_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCS1565140.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1433..1996)
                     /locus_tag="OQM52_003423"
     CDS             complement(1433..1996)
                     /locus_tag="OQM52_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1565141.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3371..5206)
                     /locus_tag="OQM52_003424"
     CDS             complement(3371..5206)
                     /locus_tag="OQM52_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565142.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5307..5594
                     /locus_tag="OQM52_003425"
     CDS             5307..5594
                     /locus_tag="OQM52_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1565143.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5566..7095
                     /locus_tag="OQM52_003426"
     CDS             5566..7095
                     /locus_tag="OQM52_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCS1565144.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7265..8482)
                     /locus_tag="OQM52_003427"
     CDS             complement(7265..8482)
                     /locus_tag="OQM52_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1565145.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8849..9835)
                     /locus_tag="OQM52_003428"
     CDS             complement(8849..9835)
                     /locus_tag="OQM52_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565146.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9832..10360)
                     /locus_tag="OQM52_003429"
                     /pseudo
     CDS             complement(9832..10360)
                     /locus_tag="OQM52_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            10427..11039
                     /locus_tag="OQM52_003430"
                     /pseudo
     CDS             10427..11039
                     /locus_tag="OQM52_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT         3407 a         2554 c         2128 g         2950 t
ORIGIN      
        1 tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac agcttcccta
       61 ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg cttacactgc
      121 aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag agaaggaatt
      181 gccttattca taccagggca aggtattaat ttaaactgta ataatttaat tttaggatgt
      241 gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc gttacaggaa
      301 agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat aggtcaaaaa
      361 ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt caaataatcc
      421 agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat atccaggatg
      481 taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc caaccctaag
      541 gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc atagtttccc
      601 agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat ttaaaggact
      661 gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt ttgccttgtt
      721 gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg aaacgagata
      781 tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca atcacaggag
      841 ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata ttcgtattga
      901 tagttctcag agatatatca cactctagat attttaatta agtatcagtt cgatatcggc
      961 atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac tgacggtgag
     1021 cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat tcgttagcca
     1081 ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg cccatacatc
     1141 atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg ataatacgaa
     1201 aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag cttcaagtaa
     1261 tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt cagatttata
     1321 aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga aattcgtgtg
     1381 aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata attcagttat
     1441 gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga ataataccgt
     1501 gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg tctaccgcag
     1561 atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct ttagcccggc
     1621 acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc agttgcctgg
     1681 atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa tgaatatgaa
     1741 gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc gtctgggttt
     1801 cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc ccgaaactgg
     1861 gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa aaacgttgaa
     1921 acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt ttgtaattgg
     1981 gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag agagaattac
     2041 gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag taaaccagca
     2101 gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga ggtaatactt
     2161 cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg gaaaaacaca
     2221 cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt aatctgacat
     2281 tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg tcgaaccatt
     2341 aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat aatggtatac
     2401 agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat atttacctat
     2461 acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac gtaaccagtg
     2521 ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc cccatatgct
     2581 tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt agcataaaga
     2641 ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac aatacggcac
     2701 agacacacaa taccataact ccaactaata atactaatta accttaatta tttatggtat
     2761 atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg atcactgtat
     2821 aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag tgaccagtaa
     2881 tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag ccactaacat
     2941 aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca tgatttggcc
     3001 caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag agcttacacc
     3061 gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca atgaacatgc
     3121 aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat ggcgctaaga
     3181 atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa aacaaaacaa
     3241 acaaataaat catactatta tagaattttc tcttgccaga catagataat acccctgcta
     3301 attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc caaaacacac
     3361 tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca caaataatgg
     3421 ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg ccatacaaca
     3481 tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat acaccaagct
     3541 tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct atcatagttt
     3601 ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc agcgtcttac
     3661 catcataaac atactcttta tctatcagaa tatttatctt atccagcacc ttctctacgt
     3721 catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg atcttttcaa
     3781 gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca tcatactgta
     3841 tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat gcatcctgat
     3901 aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt tttctattta
     3961 ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat cctgaaggta
     4021 attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca tcattaattt
     4081 tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg gtcacatgaa
     4141 cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat gcgtcatcac
     4201 aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata tattcctgag
     4261 atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg ttttgaaact
     4321 tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg gtaaagtcat
     4381 aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct ctcaccaaca
     4441 ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata tcaagtaaga
     4501 cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact ttctccagtt
     4561 catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg atagccatga
     4621 tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt cctgtatatt
     4681 tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat cccagttcag
     4741 ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta tcagcgtgat
     4801 caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa aaagcagagt
     4861 cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc ccaaaagtgc
     4921 gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga taagaattca
     4981 tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc tgactgaaca
     5041 ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag aatttttgat
     5101 gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc ttaaacaacc
     5161 ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc tgtgctctcc
     5221 ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa ctgacacaac
     5281 gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt tacacgcctt
     5341 cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga tctactattt
     5401 acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa ccaataaggc
     5461 ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg atgttacata
     5521 cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat gacgacaaaa
     5581 ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc attacttgat
     5641 gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat gctgacggtc
     5701 atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca acgtaccagc
     5761 cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc tgcctgcgca
     5821 aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata tatcgacgat
     5881 aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat cctggagcgg
     5941 agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg agcagcactt
     6001 gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat cgtgaatgac
     6061 gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct gttgctgtct
     6121 gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact gtacaacaca
     6181 ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt ccggctgaat
     6241 gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa gttttttact
     6301 ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt ttgcgaaccc
     6361 ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt gacgcaacaa
     6421 tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc tctgagagca
     6481 tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc aaatgcagca
     6541 tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca gaatcctcat
     6601 atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag gatagcagca
     6661 cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag tactaatagc
     6721 gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga atcccttcgg
     6781 gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat cacaacagca
     6841 ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct caggaaaaaa
     6901 tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag gattgatgat
     6961 aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa aaaactaaat
     7021 attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact gaagtatcac
     7081 cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt attattcgtt
     7141 ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga actggcattg
     7201 catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat gccagtccat
     7261 gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa tccgcccatg
     7321 cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc gcctcaacct
     7381 tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac ccctgttcac
     7441 tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc cccggaatgt
     7501 cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa aggatctgct
     7561 gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt tccagaatgt
     7621 aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt ctttctgcag
     7681 gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc ctggcttcgc
     7741 ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca ttataaggtt
     7801 catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc attgcgggtt
     7861 ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca tcaacaggat
     7921 ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg cgctgtttaa
     7981 cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct gccacgtccg
     8041 cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa ttctcaagcg
     8101 tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt tctgcatgta
     8161 cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca gcctttttaa
     8221 tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca gtacgtgcag
     8281 cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt ttttgtgtca
     8341 ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa ggatgaaggg
     8401 tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata tgtcttgctt
     8461 tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc atccagtatg
     8521 ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca aaaagtgtaa
     8581 tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt atttcaggcg
     8641 gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct tttttttcgt
     8701 atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa attgtgcaca
     8761 tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag aacatatatg
     8821 cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa cggaaataca
     8881 tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa aaatagtaaa
     8941 ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc cattccacaa
     9001 gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc aatatgaata
     9061 accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg gagaaaaaca
     9121 gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag aagcgcagaa
     9181 aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt atcgatttaa
     9241 acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg ctgatagtaa
     9301 gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc agacttttat
     9361 attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg cggatcatcc
     9421 gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc gcatataacg
     9481 agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac gacagaaatc
     9541 ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt ttcttaaacc
     9601 acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg agatcaaaaa
     9661 taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc tctgatggaa
     9721 aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata gcataaatgt
     9781 atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga atcatttgaa
     9841 tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat ccacgccagc
     9901 aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga aagctaccgg
     9961 ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta tgagttaagt
    10021 gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct gttacgttca
    10081 tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg cgcattgcct
    10141 gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt ttaaccggtt
    10201 cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc gttcccccaa
    10261 taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct gattccgtaa
    10321 tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc ctgatcttac ccagcaatag
    10381 tggacacgcg gctaagtgag taaactctca gtcagaggtg acttacatga caaaaacagt
    10441 atcaaccagt aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa
    10501 gcttgctgaa cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca
    10561 gctctacaac tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga
    10621 gatgtctacc gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc
    10681 tatcctccaa aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt
    10741 gaaaaacatc aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc
    10801 agcggctggt atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc
    10861 caacactgcg acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc
    10921 ccacgcctga cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg
    10981 gcaagcctgc gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcag
//
LOCUS       DAJVQR010000117        10971 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVQR010000117 DAJVQR010000000
VERSION     DAJVQR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            409..1239
                     /locus_tag="OQM52_003431"
     CDS             409..1239
                     /locus_tag="OQM52_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1565147.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OQM52_003432"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OQM52_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1565148.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OQM52_003433"
     CDS             complement(2368..3828)
                     /locus_tag="OQM52_003433"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1565149.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OQM52_003434"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OQM52_003434"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1565150.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OQM52_003435"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OQM52_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS1565151.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPAQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OQM52_003436"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OQM52_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCS1565152.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OQM52_003437"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OQM52_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1565153.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OQM52_003438"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OQM52_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCS1565154.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>10971)
                     /gene="fimD"
                     /locus_tag="OQM52_003439"
     CDS             complement(9005..>10971)
                     /gene="fimD"
                     /locus_tag="OQM52_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCS1565155.1"
                     /translation="NTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYT
                     QGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYGIYNSTVP
                     PGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYR
                     SGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMT
                     QANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGY
                     NIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNV
                     DEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRH
                     ASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYR
                     GGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVE
                     NQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEF
                     KARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWG
                     EEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2662 a         2803 c         2673 g         2833 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctggaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccagcgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg t
//
LOCUS       DAJVQR010000118        10945 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVQR010000118 DAJVQR010000000
VERSION     DAJVQR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OQM52_003440"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OQM52_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCS1565156.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OQM52_003441"
     CDS             complement(1433..1759)
                     /locus_tag="OQM52_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCS1565157.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OQM52_003442"
     CDS             1894..2235
                     /locus_tag="OQM52_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCS1565158.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OQM52_003443"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OQM52_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OQM52_003444"
     CDS             complement(2833..3543)
                     /locus_tag="OQM52_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1565159.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OQM52_003445"
     CDS             3651..3956
                     /locus_tag="OQM52_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1565160.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OQM52_003446"
     CDS             4155..4475
                     /locus_tag="OQM52_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCS1565161.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OQM52_003447"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OQM52_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OQM52_003448"
     CDS             complement(6299..7288)
                     /locus_tag="OQM52_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565162.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7861)
                     /locus_tag="OQM52_003449"
     CDS             complement(7373..7861)
                     /locus_tag="OQM52_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565163.1"
                     /translation="MMFNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLSG
                     LPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAER
                     MREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWMD
                     SL"
     gene            complement(8182..8682)
                     /locus_tag="OQM52_003450"
     CDS             complement(8182..8682)
                     /locus_tag="OQM52_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565164.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8672..9187)
                     /locus_tag="OQM52_003451"
     CDS             complement(8672..9187)
                     /locus_tag="OQM52_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565165.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9618..9986)
                     /locus_tag="OQM52_003452"
     CDS             complement(9618..9986)
                     /locus_tag="OQM52_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565166.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10398..>10945)
                     /locus_tag="OQM52_003453"
     CDS             complement(10398..>10945)
                     /locus_tag="OQM52_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1565167.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2811 a         2784 c         2702 g         2648 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccacactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgaacatca
     7861 taggtaattc tcctcttgag agttgagact atcggtatcg cagacgcagt agcgcctacg
     7921 ggtctccccg catcgctcat ctcaaaaggt ttaaaattag gtttggtatg gtcaggactg
     7981 cttagcaaag gctcggcgta actgattgtc taaatcacgg tccaccagca tagctatcag
     8041 ctcgtctcga ctgtagtaag ggcgctcagg gtcttcgact ccaagtctca gcagagacag
     8101 cagatgacgt gtgggcaggc cacggtaacg aagggtcgct ttgccgatag gtgtacgagg
     8161 gtcaatcata gccatgatta cttacctcca ctcacagcga ccagcggtca cagcttgata
     8221 gtctgaatac tcagcctgtg agacttcacg tccggcgatg tagaggcctg tgccatgacg
     8281 ctggatgaat tccttggtct ccggcttgat gtcctcagca gacgctacag cagactgcac
     8341 ggtggcgctg gaggtcatag acacgaaggc tcgggttact gccagtccgt acccaaggtc
     8401 caaccaccga ccgaactcta tagcggctac tgggtcagcc agccacgagg tccctaagtg
     8461 acccatgtta atctttttaa caaacccgtt ttctgggttg gttagacttc taccagcctt
     8521 ggtcctcatg aagcgagcaa cctcggtact acggttatct tctaccttac caatctgaat
     8581 gcctacccgg cgtatctcat ccagtcgaaa caatccgtcc tgactggtga tgtcctgacc
     8641 gaagatatga atggtgtatt cctgcttatt gtcagaagtc ataagaggca cctcccgcca
     8701 gagcttcggc aaaggacttg aagcgctgtt cttggtcagc tttcgcctga aggcgttcgg
     8761 catcaggtgg gaccagctta tcaaaggtgc cgttttcgta atcttcagtt aggacctgaa
     8821 ggcggcggga ccatttgctc tccacctgca tcatcgcgtt agagatgtgc ttcaagtcgg
     8881 catcagagcg tgacgctatc caatccatct cttctgagtc accctcacag gtgtaaggat
     8941 tgttgaaata gaagatgtcc tgcaggccac gcttcagctc ttcctcggtg tgtgtctgga
     9001 ggtatgactg gataggctcc gcgtacatct cccggatttt ctcatagtcc cagtcttccg
     9061 cctgggcata ccatcgggat aacttcagca ggtctaagtt atagcgacag caatcgacac
     9121 tggactctgc attggtcagc aggatgatta agaactggcg gttagttact ggtaattcgg
     9181 tattcataca aagatgctcc tttggtaaag cactttctgt ctccacatgg agtccgcacc
     9241 attcagacgc atcctaaacg gacccatcag aaagtgctta acgaaagtga atggttaaat
     9301 gttgacggtc tggagtgacc atgttctcca tgtacgtata aaggtgcgac cgcatgtcag
     9361 gacgagctgt aaggactcca gatggtgagc ggtgatagac ttaagccaat gtagtgagta
     9421 gacgagctac taaccgagac gactaaagaa cgctaacgag ctggaggtcg atacaggagg
     9481 tctgtcagag gcggtcacgg gccttgatta cttaggagag atacttaatg tcggttgacc
     9541 agatgctcgc atgttctgac gcatcagact cagaggcgga ggctggtctc cctatagtga
     9601 tagtttaggg tccgtaatca tgaaccagct ttactggcgt ggtactggac ggatttactg
     9661 gcgctgacga cggtagcata gacgtagcga gcgtagcgag ttgaaaccat aggttcaccc
     9721 aaggcaacac gcttacggct cagggactcc tgcacagcgc ttacagttaa tgtgtcgtgc
     9781 gttagtagtt gggtcagaat caagtatggg ctgacaggct tggtattgct tgccattgat
     9841 tcgttcaggc gaccagcacc agtaaggaca tcatccagcc aaccactcag gtcgccctga
     9901 ccatcgaatg acagcaggtc cccgcagtgc tgctgcaagt aggctttggt gaacgtgttg
     9961 tcgtgagttg cgattggtcg tgccatagag tatctcctgt agttgaatta gttggttccc
    10021 ctatagtgat aatcaagtcc atcactggtg atgcatacag tagcgcgaca ggatgtgact
    10081 cacaggttga accgaatgtc aaggactaaa gacgcctcag gaattaagga agtatatata
    10141 aagagagacc agaggcgact cacagttaaa caggaatgtc aagaggagac cataggaatt
    10201 tggagggact ggagtttagg acgcagttta aataagacca cagacagact tagagtcgga
    10261 cagccgaagg cggaccagtg ggcctatagt caggagggat tatgattatt atcaccctcc
    10321 ctaacttcag tcagacttca gtgacccaag gtctcaacca tcaagccgta tatggtctcc
    10381 accttgggtc tccctactta aagtaaagct gtcttaagca catcagccag cctacccaca
    10441 gcgtgtcctt tcccgtacag gtcatacgta attgactgac tgctgtgacc taagatgcct
    10501 tgagcgtcca ccagattgac tccacgctct ttcattcggg tcgccagcca atgtctcaga
    10561 ctgtggagag agacatcctg agagtctcct agggcctcag tcaggaccct tgagttaaac
    10621 cagctagagt aggcgctcgg agtcatcccg aagagaggac cctcatcagg ctgcatgtct
    10681 acccacgaca ggaaggacgt caggtcaaac ccataagcgc catcggttaa cggcacaagt
    10741 cggactgaat gtttgttctt cacagacttc ccgtccccat cttcattgat gtcgatacag
    10801 accagtccat tgtccaaggt tacaatgtca cgtttagtca ggtggcagac ctcagcagac
    10861 cgagcgccag tgaccacaga caccagagac gcccatctac gggcctcaga ggtcgtgtga
    10921 gccgtgtgtc gtgtgaactg atact
//
LOCUS       DAJVQR010000119        10926 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVQR010000119 DAJVQR010000000
VERSION     DAJVQR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            154..504
                     /locus_tag="OQM52_003454"
     CDS             154..504
                     /locus_tag="OQM52_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS1565168.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OQM52_003455"
     CDS             557..952
                     /locus_tag="OQM52_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1565169.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OQM52_003456"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OQM52_003456"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1565170.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OQM52_003457"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OQM52_003457"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1565171.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OQM52_003458"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OQM52_003458"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS1565172.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OQM52_003459"
     CDS             complement(5323..6423)
                     /locus_tag="OQM52_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1565173.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OQM52_003460"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OQM52_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1565174.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OQM52_003461"
     CDS             complement(7571..8137)
                     /locus_tag="OQM52_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1565175.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OQM52_003462"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OQM52_003462"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1565176.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="OQM52_003463"
     CDS             10263..10751
                     /locus_tag="OQM52_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCS1565177.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2788 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAJVQR010000120        10818 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVQR010000120 DAJVQR010000000
VERSION     DAJVQR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            161..1615
                     /gene="amn"
                     /locus_tag="OQM52_003464"
     CDS             161..1615
                     /gene="amn"
                     /locus_tag="OQM52_003464"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1565178.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            1958..2674
                     /locus_tag="OQM52_003465"
     CDS             1958..2674
                     /locus_tag="OQM52_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1565179.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3130..3205)
                     /locus_tag="OQM52_003466"
     tRNA            complement(3130..3205)
                     /locus_tag="OQM52_003466"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3170..3172),aa:Asn,seq:gtt)
     gene            complement(3306..4933)
                     /gene="yeeO"
                     /locus_tag="OQM52_003467"
                     /pseudo
     CDS             complement(3306..4933)
                     /gene="yeeO"
                     /locus_tag="OQM52_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4938..5013
                     /locus_tag="OQM52_003468"
     tRNA            4938..5013
                     /locus_tag="OQM52_003468"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4971..4973,aa:Asn,seq:gtt)
     gene            complement(5051..6001)
                     /gene="cbl"
                     /locus_tag="OQM52_003469"
     CDS             complement(5051..6001)
                     /gene="cbl"
                     /locus_tag="OQM52_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS1565180.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6103..7020)
                     /gene="nac"
                     /locus_tag="OQM52_003470"
     CDS             complement(6103..7020)
                     /gene="nac"
                     /locus_tag="OQM52_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS1565181.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7348..7423
                     /locus_tag="OQM52_003471"
     tRNA            7348..7423
                     /locus_tag="OQM52_003471"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7381..7383,aa:Asn,seq:gtt)
     gene            complement(7479..8414)
                     /gene="ldtA"
                     /locus_tag="OQM52_003472"
     CDS             complement(7479..8414)
                     /gene="ldtA"
                     /locus_tag="OQM52_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1565182.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8476..9555)
                     /gene="cobT"
                     /locus_tag="OQM52_003473"
     CDS             complement(8476..9555)
                     /gene="cobT"
                     /locus_tag="OQM52_003473"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1565183.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9567..10310)
                     /gene="cobS"
                     /locus_tag="OQM52_003474"
     CDS             complement(9567..10310)
                     /gene="cobS"
                     /locus_tag="OQM52_003474"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1565184.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10307..10816)
                     /gene="cobU"
                     /locus_tag="OQM52_003475"
     CDS             complement(10307..10816)
                     /gene="cobU"
                     /locus_tag="OQM52_003475"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS1565185.1"
                     /translation="MVVSAIASTPHSSQVLYIATSQILDDEMAARIEHHRQSRPEHWR
                     TVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINA
                     EIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLV
                     VSGIGVKIK"
BASE COUNT         3023 a         2753 c         2335 g         2707 t
ORIGIN      
        1 ctggatgcct gatttctgca atgaccgctt tattgatgaa agacaatcaa cgctcgtgat
       61 agcctggcga agatcatccg atcttcgcct tatacttttg tttcacattc ctgtgacata
      121 ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg
      181 tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagttgc
      241 tttacgcaac gccattggca aatatattac aattggcgaa ttacctgatg aaaacgcccg
      301 taaacaaggt ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaacaaa
      361 tccccccaaa acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat
      421 tactcgccct actctctttc gttcgtatct gaatgagcaa ctaacgttgc tttatcagga
      481 ttatggtgcg catatctctg tgcaaccctc gcaacatgaa atcccttatc cttatgtcat
      541 cgatggctct gaattgacac ttgatcgctc aatgagcgct gatttaactc gctacttccc
      601 gacaacagaa ctggcgcaaa tcggcgatga aactgcagac ggcatttatc atccaacgga
      661 attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cacggttgcg
      721 ccattatacc ggtacgccag tagaacattt tcaaccgttc gtcttgttta ccaactacac
      781 acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc
      841 ctacattgcc ctttcttgtg ctggcgggaa ctggatcact gccgaaaccg aagcgccaga
      901 agaagccatt tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac
      961 cgctgatggt cagggtatta ctctggtgaa tattggcgtg ggaccgtcca atgctaaaac
     1021 catctgcgat catctggcag tgctacgccc ggatatctgg ttgatgattg gtcactgtgg
     1081 cggattacgt gaaagccagg ccattggaga ttatgtcctt gcacacgctt atttacgcga
     1141 tgaccacgtt cttgatgcgg ttctgccgcc cgatatacct attccgagca ttgctgaagt
     1201 gcaacgtgcg ctttatgacg ctaccaagtt agtcagcggc aggcctggtg aggaagtcaa
     1261 acagcggctg cgtacaggta ctgtggtaac cacagatgac aggaactggg agttacgtta
     1321 ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc
     1381 aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc
     1441 agataaaccg ttacatggtg agattaagct tcccggccag gctaaccgtt tttatgaagg
     1501 cgctatttcc gaacacctgc aaattggcat tcgggcgatc gatttgctgc gcgcagaagg
     1561 cgaccgactg cattcacgta aattacgaac ctttaatgag ccgccgttcc gataataagt
     1621 tatgaacctt cttatcagaa atagatgtga aggattaagt aagacatctg gcaaatagcc
     1681 tgcaataaac gcgggctgct tgcttccttg ggcagatgcg agttttatta tcgtcttaat
     1741 gatttccaca tattaaaagc aagtatgctt tcaaaacaca attataaaaa atcccgccaa
     1801 caacattagt ttttataaaa ttaaatataa gattatggct ttagaatatt tttatttcta
     1861 atagacgaga tttttcctgt tatgatataa tatgccgaat taacacatgt taacgattta
     1921 ccagtaatgt aaataaattt tcgaggagat cattccagtg ggacgtaaat gggccaatat
     1981 tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa aattcggtgt
     2041 agaaatctat gctgctgcca aacaaggtga acccgatcca gaattaaaca catctttaaa
     2101 attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg ataaagcaat
     2161 tgataaagcc aaaggcggcg gagatgaaac gttcgtacag ggacgttatg aaggctttgg
     2221 tcctaatggc tcaatgatta ttgctgagac attgacttca aatgttaacc gtacgattgc
     2281 taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag gttctgtcag
     2341 ctatatgttt gacaatacgg gtgtgattgt atttaaagga acagaccctg accatatttt
     2401 tgaaattttg cttgaagctg aagttgatgt tcgtgatgta actgaagaag aaggtaatat
     2461 tgttatttat actgaacctg ctgaccttca taaaggaatc gcggcgctaa aagcagctgg
     2521 aatcactgag ttctcaacaa cagaattaga aatgattgct caatctgaag ttgagctttc
     2581 cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg acgacgatgt
     2641 acaaaaagtt tatcataacg tcgcaaatct ctaattatat tttaaagaaa tctgtcttta
     2701 tggcagattt ctttaatctc atataattct tataaaaaat ataatattta actcgtcata
     2761 ttgcttataa aaaatccccg tccccagaga aataatattt attaaaatgg caggtcttct
     2821 ttttctgatt accgaagaca aagtggcaat tacgtatagt ttcccgataa agacgcgaca
     2881 gctacgtccc gcataaggca tttttctctt tatctttgta cggtacttca tggaacagaa
     2941 tttttgacct tgtgaatcgt gatatctgtt ggggaggggc aatttgctca ctgaagcgtg
     3001 agactcgatt aagcgcacga aacacagaaa tcaaaaaacc cggccacttt tttacaaagt
     3061 ggccgggtaa aaataatttt tattttttaa ctgtcctgag tctcatagag atgtctcaaa
     3121 actaaaattt ggctcctctg actggactcg aaccagtgac atacggatta acagtccgcc
     3181 gttctaccga ctgaactaca gaggaatcgt gagaacgagg cgaatattag cgatgcccac
     3241 ccacaatgtc aaagcctgtt ttttaaattt gaaatcgttt gctgaaataa tctgcatttt
     3301 gtcgtttatt ccgacacaac tggctttttt tcacactttt gcggctcgga tcgagggtat
     3361 ttccacagcc aacgtccagt aaccattcgc cagtaaaaca gcacggcccg cacagcccag
     3421 tcggcaaaca ttcccatcca gacaccaacc acaccccagc caagcatgat tccctgcaca
     3481 taaccgacta caacccggca accccacatg ctcaacatag aaacccacat ggcgtaacgg
     3541 gcatcacgag cacccttaaa tccagcgggt agcacccatg aggcggacca aataggcata
     3601 aataaagcat ttagctaaat cagaatcaca acgacgtgtt taacctgtgg atcctgggtg
     3661 taaaacgatg ccataacccc ggcaaaggga gccgttagcc aggcgatggc cgttaatcca
     3721 agagtggaca gccagaacac atgccgcaac tgaatctctg cttgcgctat ctgccctacc
     3781 cccaaccttc ggcctgtaat gatcgtagaa gcagaaccga gcgcacttcc gggtaagttg
     3841 ataagagccg caattgagaa cgcgataaaa tttccggcaa taacgctggt ccccatcccg
     3901 gcaacgaaca tttgggttaa taaccgacca ctggtaaata acactgattc gacactcgct
     3961 ggaataccaa tccccatgac ttcccagata atgctaaaat tcagcggttt aaaatagctc
     4021 tttaacgaaa tccttagcgc aggattaaaa ccaatcgcca gcacccacaa aattgcaact
     4081 gcgccaatat aacgagaaat ggttaaaccc aacccagccc cgaccaggag aaaaggccgt
     4141 aaatcaatat gccgctaata ataatattaa gaatattcag gctaccgtta atcagtagcg
     4201 gtattttcgt attccctgca ccacgaagtg ccccgctacc aataagagtg atggcagcgg
     4261 ctggataact gagtacggtc agctccagat aagtcaacgc cagtgcttta acttctgtcg
     4321 tggcatcacc cgcgacgaaa tcaataattt gttcgccaaa atgatgaatt agcgttgcca
     4381 acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc
     4441 gtcgatcccg cttaccgaga ctaaatgcta caacgacagt agtaccaaga tcgatagctg
     4501 caaaaaaagc cataatgacc atattgaagc tgtccgccaa ccccacgccg gccatcgcat
     4561 cttttcccag ccaactgacc agaaaagtgc tcaggacccc catcaacagg acacaggcgt
     4621 tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ctagaacaac actttgtagc
     4681 tcttgcgttt agagtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat
     4741 tcaaagccga ccttaattgc agaaagtgaa accacatttc aaatgatgag ggagaatcag
     4801 caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa
     4861 acggcaaaca gttgggaaat ttaaaaatcg ggtttgacaa aatatttttc gccgttaaga
     4921 tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     4981 atgtcactgg ttcgagtcca gtcagaggag ccaaatttct gaaaagcccg cttttatagc
     5041 gggctttttg ctatatctga taatcaattt cctcttcact gttttccatc acctggcgct
     5101 tgatatcctc aactgacagt cctgcattac aaagttccag aaagcgccaa acatagttac
     5161 gctgaagttg tcctcgcttc agtcccaacc agacagtatt agcatcgaaa agatgccgcg
     5221 tatccaggcg gattaaattc tcttcctctt gttcgccgct ggattgttcg gcaactaatc
     5281 cgatcccaag cccaagagca acataggttt taatgacatc agaatcctgt gcacttaata
     5341 caatatctgc cggcaaacct ttgcgggtaa atgcgtcatc agtgcgtgag cgccccgtaa
     5401 ttccctgtcg gtaagtaatt aacggccact tcgctattga ttccagcgtc aatggtgtaa
     5461 tttgcgttaa gggatgatcg agtggaacaa gcaaactatg gtgccaacaa aaccatggga
     5521 aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc
     5581 cattttgcaa caacgtcgca atttcctgtg gcgaaccctg gattagctcg agccgaacct
     5641 ccgggaaaag ttcgcgaaaa gctttaatga cctctggcaa gctataacgt gcctgagtat
     5701 gcgtcgttgc aatagtgaga acaccagacg tatcgttggt aaacaggtct gcaagccgac
     5761 gaacattact ggcttcattc agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt
     5821 cagtcatccc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca aattcatcct
     5881 ccagttcccg aatatgacgg ctaacgcctg actgtgaggt aaaaagcata ttcgcaacct
     5941 ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat ctttagttgt tggaaattca
     6001 cggtaaactc cgggcagttc agatatcccg ttattgttaa agtctaatgc ccggcataac
     6061 aaataataaa aacccgcatc ttattccatc ccgatataac gcttagctca ccaattgcca
     6121 ctgccttttt tccatcactg gagaactaat cactgacatt aacaactctt tcactgcctg
     6181 tgcctgtggt gacaagtttg ctctggcggg taaatttaag gacaaagaga gactcattga
     6241 aggagtggta atgcgtgaca tccacccatt tactgcgcca cataacgaac gcgcagccga
     6301 ttcaggtaat actgcaacgc ccatgccgct ggcaatcgct gcggtaagcg tggcaataga
     6361 ctcaatttca ccaatgactt ttgccgtgag ccgccgtagg gaaaaagcct catcaacacg
     6421 aagtctaaca gcgctgtaat cactggggag aaaaaggttc atttgcgcaa tagcattaac
     6481 atcaacgttt tgccccgggc aatcttgagt tcctaccagg aaaagatctt ctttcagcaa
     6541 agcctgactg gataccccag ccacagggga atgctcataa atcaccgcca tatcgagttg
     6601 gtgatttatc agtttttcgt taagcactgc accactattt tcatgaagat agataacgac
     6661 ctccgggaat tcagcgcgaa ccgcctgtaa taagggcatg gtgatggatg acgcagcggt
     6721 tcctggcgca aagccaatcg agacttgccc cgataatgac tgaccaacgt tatgcaccgc
     6781 cagttgggcc tgctcacact gacgtaaaat ggcccgtgca tgggtataga gaatttttcc
     6841 ggcgtctgtt ggcgtaacgc cccgttttgt gcgaatcaaa agctgttgat ttaactcacc
     6901 ttccagtatg gcaacctgtt ggctgagcgc tggttgtgcg atatgcaata cttcagcagc
     6961 ctgggtcagg ctaccaatat ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat
     7021 gttgcctccg gtttttaaga atcggcccaa gtgccgccat tacttacaac cagtatgcaa
     7081 gatgcctgcc agttctattt tggcgttgat gcgcaagcta accaactgtc aaataagaga
     7141 ttatgataga ttcgtcattg gcccttttta tcagcagtcg cattcacctg ccctataaaa
     7201 ggagggtatg caccacgatg gttcattacc caataagatt gaaagctcac cactttgttg
     7261 aaattggcag caaacaaaca aaaaaaatgc atttcaccct ttgacatcac catgcactgc
     7321 cattaatatg cgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7381 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcaa aaaagcctgc
     7441 tttcgagcag gctttttact ttctaattac caacgctctt aaaacatctg tcttgaacca
     7501 gaactaattt gcacaggcat tcccgatcga tgttgcaacg cagcatttgc gcgatttaca
     7561 tcaacttctt gcccgttgat aaacgcccgc aaagatgggg ttaccggcaa tggcactttt
     7621 cggtcagact catattctgc acgattgcgc gacagcggct catgaacttc cagccacttt
     7681 gaaccatccg gttccgttgt gtatttcact ggctgatcaa taatttgtac acgagtgcct
     7741 acaggaacat tatcaaacag atatttgata tcgtcattac gcagacgaat acagccctga
     7801 cttacacgga gcccaatacc aaaattggca ttggtaccgt ggatggcata caacctgcca
     7861 atataaatcg cgtacagccc catggggtta tcgggccccg caggaacaaa tgcgggcaaa
     7921 ctctcccctc gtttcgcata ttcgcgccgg atgttcggcg ttggcgtcca ggttggtgct
     7981 tcttgtttac gttcaacggt agtcacccag ttacgcgggg tttctcgccc agcctggccg
     8041 ataccaatag gaaagacttc cactgtatta ctgtctggcg gatagtaata aagacgcatt
     8101 tcagcgacgt taaccacaat ccctttacga acagtggcgg gcaaaatcag ttgctgcgga
     8161 atggtgagtt gtgagccagg cttcggcaaa aaaacatcag cgcccggatt cgcttccagc
     8221 atattactta acccttgccc gtattgtgcg gcaaaagtct ccagcggctg agtattgtga
     8281 tcaggaacag ttacagtaag cgactgcccc actaaacggc taccctctgg aggtaatgga
     8341 taagttaccg ccaggctatt ttggctggca aaaagcagag caaatgagca aagaatattt
     8401 acacgacgca tcatgtccct ttcctaagtc gcgaatacta tccgataagt atagctttta
     8461 tcagactttt cgtttttagc tgttcaaatc agaagtcgta ttccccggta gaacaatatt
     8521 actggcagca agttcgccca tgttgttgta tatcgcacaa gcagcttcga tgatggacat
     8581 cgccagagct gcaccactcc cctcacctaa acgcatatcc atattgagat aaggctccag
     8641 ccccaaatgc gagagcgcta tacgcgcgcc tttttctgcc gacaagtgag aaggaatgag
     8701 atacggtttg attgcaggag acatctggca ggctgcgagc gcagcagcat aagaaagaaa
     8761 tccatccagc aacacgggta aaccgcagga agcagcacct aacatcactc cagctattcc
     8821 gaccaaatca aatccaccca cttttgccag gacatcaaca ccatcctgag gatttggttg
     8881 attcaacgta atcgcccgac gcacaacatc aattttatta gccagtttat ctgtcggcag
     8941 gtttgcgcca atcccaacca cttcttcagg atcccggcca gtgattgtgc tgactattgc
     9001 cgctgccggt gtcgtgtttg ccatccccag ttcacctaca ccaaacagcg tgacaccgtt
     9061 tttcgccagc tcccgcgtat aacatatgac gtccaaaagc aacttttcag cctgacggcg
     9121 actcattgcc ggagctgaag caatattgcc gctacctcgt gcgacacgca tgttgataag
     9181 cccggggata ggctcagcag tatcaatacc aacatcaatt acgtggacgt tagcgcccgc
     9241 ttgtgctgcc agcacacaca cgccggttgt tccacgggtc atattttcag cctgtatggc
     9301 tgtcacttct tttggagaaa tagcgacccc ttcctcccag acgccgtgat cggcacacat
     9361 aaccagtacc gcttttttgc ccacatgcgg tatgccattc acccccggca ttcctgccag
     9421 ttgtatggca agcgcctcca gctttcctag gctaccaaca ggtttgaata acccgtcaac
     9481 atgccgttgt gcacgcgaca tagcggcaga attgatggct gggatcgtat tcagtaaatc
     9541 ggcaattatt tgcatgtcac gtttctttat agcagtgcca gcaggaagac cagttcacca
     9601 agctctatgg ctgctcccag cgtatcgccc gtttgcccgc ccagcgtacg tttgagaagc
     9661 tgaccgagga tgaaaatcgc caccatcgtc accaccatag cagccacacc atgcataccc
     9721 agcaataata cggcagcaaa aatagcggct aagccgagtg tgacgcaggt ttgtcgccca
     9781 tcaattttgc caatgaacac attgccaagc ccttcttccc gcgcataacg atgacgatac
     9841 atcaataatg cggcagtccc gcggctgacc gcacatgctg ccgccagcga tgcaaggatc
     9901 ggttcgccac gcagagccaa ctcgcttaac accagaatct ttgccagtac cacaaaaatc
     9961 aatgccagac cgccgtgggt gcctaaacgg ctatcacgca tgatttccaa catgcgatcg
    10021 cgactacgtg cagaaaatac gccatcacag gtatcagcga ggccgtccag atggaattcc
    10081 ccggtcatca gcgccagcac aagtacgcta aacagtgccg ccagtggtgc gccacaccat
    10141 gcctgcagca ccatgaagac cagcccgcta atcgcgccaa gcaataatcc aatcaaagga
    10201 aaagtaataa taccgcgaga ataatgttcg aaatccagtc cctgggacca gcgacgcggt
    10261 acaggcagac gcgtaataaa tgagagcatc gcccaaaata atttgctcat ttgattttta
    10321 ctccaatacc cgaaaccacc agccatactt catttgccgc agcggccaac tgctgattta
    10381 cccgcccggc aatatcacga aaatgtcgtg ccagacgact ctccggcaca atccccattc
    10441 ccacttcgtt agtcactaat acaacctttg cggggcaacg ttggcaggca gcaatcaacg
    10501 actgaatctc agcattaatc gcctgttcca tcgcctgata atcccattca tcagggtctt
    10561 tatcgccgcc ataatcaaac aacagattag tcaccattgt ggtaacgcat tccagcaaca
    10621 cagcctcatt cgggttaatg tctgcatgaa ttaattcatc aagatgttgc cagcgctcca
    10681 ctgtgcgcca gtgctccggg cggctttgcc gatgatgttc tatccgtgca gccatctcat
    10741 catcaaggat ttgcgaggta gcgatataca gaacctgtga agagtgaggt gtactggcaa
    10801 tagcggacac taccattt
//
LOCUS       DAJVQR010000121        10814 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVQR010000121 DAJVQR010000000
VERSION     DAJVQR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..285
                     /locus_tag="OQM52_003476"
     CDS             <1..285
                     /locus_tag="OQM52_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565186.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWM
                     ESTIFPALNDVPALSGLIDTLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OQM52_003477"
     CDS             296..1036
                     /locus_tag="OQM52_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1565187.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OQM52_003478"
     CDS             1095..1481
                     /locus_tag="OQM52_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1565188.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OQM52_003479"
     CDS             1490..1819
                     /locus_tag="OQM52_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1565189.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..4832
                     /locus_tag="OQM52_003480"
     CDS             1791..4832
                     /locus_tag="OQM52_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1565190.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4832..5161
                     /locus_tag="OQM52_003481"
     CDS             4832..5161
                     /locus_tag="OQM52_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565191.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5161..5859
                     /locus_tag="OQM52_003482"
     CDS             5161..5859
                     /locus_tag="OQM52_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1565192.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5864..6607
                     /locus_tag="OQM52_003483"
     CDS             5864..6607
                     /locus_tag="OQM52_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565193.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6544..7146
                     /locus_tag="OQM52_003484"
     CDS             6544..7146
                     /locus_tag="OQM52_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1565194.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7207..10686
                     /locus_tag="OQM52_003485"
     CDS             7207..10686
                     /locus_tag="OQM52_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1565195.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10754..>10814
                     /locus_tag="OQM52_003486"
     CDS             10754..>10814
                     /locus_tag="OQM52_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCS1565196.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2547 a         2562 c         3463 g         2242 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgctcag tacaccggaa aaatgacgga tgaagatgac
       61 tggcaggctg ttctgcatat tgccgtcttc atccgggcac aggcaccgga ttcagagctg
      121 gatatgtgga tggagagcac cattttcccg gccctgaatg atgtaccggc actttccgga
      181 ctcatcgaca ccctgaaccc actcggtttt aactatcaac gtgataatga gatggctacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg
     4621 atggtaagct gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc
     4681 agtacaacat tgagatcaat aacgacggca cgaacgggca gataggtccg gctgccctga
     4741 aggtggttta tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg
     4801 atggtggccg gttaagtgga gcataccggt aatggagacg tttcactgga aagtgcgccc
     4861 ggatatgaat gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga
     4921 acagcgtcgt gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt
     4981 tcgtaaaggt gaacacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc
     5041 atttcagtgg acgccacctt atgactggaa actgatcagg gtggtttgtc gtaaatggtc
     5101 ggcaagcgtg ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg
     5161 atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcagcccgg
     5221 gtggagctct gggaaatcga tctgacagag gtcggtggtg agcgttattt tttctgtaat
     5281 gagcagaacg aaaaaggtga gccggtcacc tggcaggggc ggcagtatca ggcatacccc
     5341 attcaggggt cggggtttga actgaacggc aggggctgtg ctgcccgtcc gacactgacg
     5401 gtctctaacc tgcacggcat ggtcaccggg atggcggaag atctgcagag tctggtcggc
     5461 ggaacggtgg tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac
     5521 ggaaacagcg acgccgatcc ggaacaggag gtgatcagcc gctggcgcat cgagcagtgc
     5581 agcgaactga gcgcggtcag tgcctctttt gtgctggcca caccaacgga gacggatggc
     5641 gcggttttcc cggggcgtat catgctggcg aatacctgta tgtggactta ccgttctgat
     5701 gagtgtggtt acacgggcag ggcagtggct gacgagttcg acaaaccaac aacggatatc
     5761 cggaaggaca aatgcagcaa gtgtatgcgc gggtgtgagt tgcgcaacaa taccggtaat
     5821 ttcggcggtt tcctttccat caataaactt tctcagtaaa tccatgacac agacagaatc
     5881 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgtggct tcgtggtgag
     5941 aacgccggag ggagacaggt atcttcccag cgagaatatc tccggtgagc cggaggaacg
     6001 gttccggatg gctccggagg actggctgcg ggcacaaatg cagggtgaga ttgtggcact
     6061 ggtccacagt catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt
     6121 gcagagtgat ttgccgtggt ggctggtctg ccgggggacg attcacaagt tccgctgtgt
     6181 gccgcatctc accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     6241 ggatgcttat catctggcgg ggattgagat gccggatttt catcgcgggg atgactggtg
     6301 gcgtcacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc
     6361 gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa
     6421 tcatgccgcc atttactgcg gcgatggtga gctgctgcac catattcctg aacaactgag
     6481 taaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
     6541 ggaatggcac gcatctgcct ttacggggat ttgcaacgat ttggccgccg catcgacctt
     6601 cgtgtgaaaa cgggggctga agccatccgc gcactggcca cacagctccc ggcgtttcgt
     6661 cagaaactga gtgacggctg gtatcaggtg cgcattgccg ggcgtgatgc aggtgaaacc
     6721 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg cagtgatcca catagtaccg
     6781 cgtctggtgg gagctaaaag tggcggtgtg tttcaggcgg tgctgggggc agctgttatg
     6841 gcggttgcta tatggatgcc gggggtagga attatggcga gtaatctgct gttttctctc
     6901 ggtgccagta tgacgcttgg cggtgttgca cagatgctgg caccgaaagc cagaactccc
     6961 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
     7021 gttgcccagg gcaatgttct gcctgttctg tacggtgaaa tgcgcgtggg gtcgcgggtg
     7081 gtatctcagg agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt
     7141 cgctgatgca aaatgtttca tgtgaaaccg cctgcgggcg gttttgtcgt ttatggagcg
     7201 tgaggaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
     7261 aaatccacgc agttgctgag tgtgattgat gccatcagcg aagggccggt tgacggtccg
     7321 gtggatggat taaaaagcgt gctgctgaac ggtacgccgg tcctggatag cgaggggaag
     7381 acaaactttt ccggtgttac ggtggtgttc cgcgccggcg agcaggagca gacaccgccg
     7441 gaggggtttg aatcttccgg ctcagagact gtgctgggta cggaagtgaa atacgacacg
     7501 ccgatcaccc ggaccatcac gtcggcaaac attgatcgtc tgcgcctgac cttcggtgtg
     7561 caggcactgg tggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     7621 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     7681 accacttcgc agtatctggc ctcggtggtg gtgggtaacc tgccgccgcg cccgtttaat
     7741 atccggatgc gcaggatgac accggacagc accacagacc agctgcagaa caaaacgctc
     7801 tggtcgtcgt acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgctggtc
     7861 ggcgtgcagg tggattcaga gcagttcggt aaccagcagg tgagtcgcaa ttatcatctt
     7921 cgcgggcgca ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt
     7981 atctgggacg gaacgcttaa gccagcatac agcaacaaca tggcctggtg tctgtgggac
     8041 atgctcactc atccgcgcta cggcatgggg aaacgtcttg gtgcggcaga tgtggacaaa
     8101 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg atttggcggc
     8161 acggagccgc gcatcacctg taacgcttac ctgaccacac agcgtaaggc gtgggatgtt
     8221 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     8281 ttcgtgcagg accgaccgtc ggataaagtg tggacctata accgcagtaa tgtggtgatg
     8341 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgtc
     8401 gttgaggtga actggattga tccggacaac ggtcatgaga cggcgacaga gcttgtggag
     8461 gacacgcagg ccattgtccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     8521 accagccggg gacaggcgca ccgcgccggg ctgtggctga ttaaaacgga actgctggaa
     8581 acgcagaccg tggacttcag cgtcggcgca gaagggcttc gccatgtacc gggtgatgtc
     8641 attgaaatct gcgatgatga ttatgccggt atcagcaccg gtggtcgcgt actggcggtg
     8701 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccgtc ctccggtacc
     8761 acgctgataa gcctggttga cggaaatggc aatccggtca gcgtggaggt tcagtccgtc
     8821 accgacggcg tgaaggtgaa agtgagccgg gttcctgacg gcgttgcaga atacagcgtg
     8881 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     8941 aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg tgccggaaaa agaggccatc
     9001 gtggataacg gggcacactt tgacggtgac cggcgcggca cggtgaatgg tgtcacgccg
     9061 ccagcggtgc agcatctgac cgccgaagtc accgcagaca gcggggaata tcaggtgctg
     9121 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     9181 gcagcggacg acggcagtga gcggctggtc agcacagcca ggacgacgga aaccacatac
     9241 cgcttcaggc agctggcgct ggggaattac agtctgacag tccgggcggt aaatgcccgg
     9301 gggcagcagg gcgatccggc gtcggtatcg ttccggattg cggcaccggc agcgcctgtc
     9361 actattgaac tgataccggg gtattttcag ataacggtgg tcccgaaact ggctgtatat
     9421 gacccgacgg tgcagtttga gttctggttt tcggaaaagc ggattgcgga tatcaggcag
     9481 gttgaaacca gcgcccgcta tcttggcacg gcgctgtact ggatagccgc cagtatcaat
     9541 atcaggccgg gccatgatta ttatttttac gttcgcagtg tgaacaccgt tggcaaatcg
     9601 gcatttgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct gagtttttat
     9661 aaagggttga tcaataaaac gcatctcggc aaggagttgt ggacgcagat tgataacggt
     9721 cagcttgcgc cggacctgac tgaaatcagg acgtccatta cgaatgtcag caatgaaatc
     9781 acgcaaaccg tcaataaaaa actggaaaat cagagtgctg caatccagca gatacagaaa
     9841 gttcaggttg atacaaataa taacctgaac agcatgtggg ctgtgaagct gcaacagatg
     9901 aaggacggac gcctttatat tgcgggtatc ggtgccggta ttgagaatac gccagcagga
     9961 atgcagagtc aggtgctgct ggcggcagac aggattgcga tgattaatcc tgcgaatggc
    10021 aacacaaagc cgatgtttgt tggtcagggc gatcagatat tcatgaacga cgtgttcctg
    10081 aaacgcctga cggctccgac cattaccagc ggcggtaatc ctccggcatt ttccctgaca
    10141 cctggcggac ggctgacggc gaaaaatgcc gatatcagcg gtaacgtgaa cgcgaactcc
    10201 gggacgctca acaacgtcac gattaataag aactgtcggg ttctgggaaa attgtccgcg
    10261 aaccagattg aaggcgatct cgttaaaaca gtgggcaaac ctttcccacg ggactcccgg
    10321 gcaccggaga ggtggccatc agggaccatt accgtcaggg tttatgacga tcagccgttt
    10381 gatcggcaaa ttgttattcc cgcggtggcg tttcgcggtg ctaaacatga gcggaagaat
    10441 aacaatattt attcgtcatg ccgcctgata gtgaagaaaa acggtgctga aatttataac
    10501 cgaacgaccc tggataatac gctgatatat acgggtgtta ttgatatgcc tgccggtcac
    10561 ggtcacatga cgctggagtt ttctgtatcg gcatggctgg taaatggctg gtatcccaca
    10621 gcaagtatca gcgatttgct ggttgttgtg atgaagaaat ccacagcagg tatcagtatc
    10681 agctgaattt tataacccag atacgggcac cagaaatggt gcctttttta ttgcagaaaa
    10741 gcgagaggta attatgcgta aactttatgc tgccattttg tccgcagcca tctgtctggc
    10801 tgtatccggt gcgc
//
LOCUS       DAJVQR010000122        10585 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVQR010000122 DAJVQR010000000
VERSION     DAJVQR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..157)
                     /locus_tag="OQM52_003487"
     CDS             complement(<1..157)
                     /locus_tag="OQM52_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1565197.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTV"
     gene            complement(319..393)
                     /locus_tag="OQM52_003488"
     tRNA            complement(319..393)
                     /locus_tag="OQM52_003488"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Arg,seq:cct)
     gene            complement(467..1399)
                     /locus_tag="OQM52_003489"
                     /pseudo
     CDS             complement(467..1399)
                     /locus_tag="OQM52_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1693..2448
                     /gene="mlaA"
                     /locus_tag="OQM52_003490"
     CDS             1693..2448
                     /gene="mlaA"
                     /locus_tag="OQM52_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1565198.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2630..3688)
                     /locus_tag="OQM52_003491"
     CDS             complement(2630..3688)
                     /locus_tag="OQM52_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565199.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4054..5394)
                     /gene="fadL"
                     /locus_tag="OQM52_003492"
     CDS             complement(4054..5394)
                     /gene="fadL"
                     /locus_tag="OQM52_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1565200.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            5766..6050
                     /locus_tag="OQM52_003493"
     CDS             5766..6050
                     /locus_tag="OQM52_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1565201.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6230..7540
                     /gene="fadI"
                     /locus_tag="OQM52_003494"
     CDS             6230..7540
                     /gene="fadI"
                     /locus_tag="OQM52_003494"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1565202.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7540..9684
                     /gene="fadJ"
                     /locus_tag="OQM52_003495"
     CDS             7540..9684
                     /gene="fadJ"
                     /locus_tag="OQM52_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1565203.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            9887..10372
                     /gene="sixA"
                     /locus_tag="OQM52_003496"
     CDS             9887..10372
                     /gene="sixA"
                     /locus_tag="OQM52_003496"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1565204.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2635 a         2442 c         2655 g         2853 t
ORIGIN      
        1 cgacggtcag gattttctcc ttaccgtcaa tcttgtagcg aagctgccat acctttttcc
       61 cggatacagg gacataaagg tacaggccat taccatcgag aaggcggtat ggtttttctt
      121 tcggctttgc tgcttcaatc tgcttaacgg tgagcatggg taaaaatccg gtgggtaaaa
      181 ttattttatc cactttttac ccgtcatgga gtgcggctgt caacgatctg acgcgaacca
      241 tgacgaactg tgaatatacg gaaggcttga tattcagggg attttgcgga ctggtacgga
      301 tgggagcgaa ctgataaatg gtgtcccctg caggaatcga acctgcaatt agcccttagg
      361 aggggctcgt tatatccatt taactaagag gacaatgcgg cgagtatacc cgctaatgga
      421 ctgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gctgggtttt
      481 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
      541 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
      601 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
      661 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
      721 cacccagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
      781 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
      841 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
      901 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
      961 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     1021 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     1081 ttatgacgat aataaaacca aaggtataac cgagattctc cagcaagaag ctgcccggca
     1141 caccttccag ttcggcatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     1201 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttctagctct ttttcaccat
     1261 cctggcggat atgctcatga attgccatcg ctcgggaggg gagtcggtct tcatctattt
     1321 ctattttttt gccgcgctct ttttcttcgc tctaaacttc aatttcgtcg ctgtgttgat
     1381 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     1441 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     1501 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     1561 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     1621 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     1681 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     1741 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     1801 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     1861 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     1921 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     1981 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     2041 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     2101 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     2161 acgctgcgtg atgacggtgg tgatatggcg gatgctcttt acccggttct ttcctggctg
     2221 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     2281 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     2341 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     2401 aacgcgcaag cgattcagga tgatttaaaa gatatcgatt ctgaataaga aacaaataaa
     2461 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     2521 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt tgtcactgac
     2581 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     2641 caaaattaac acctagtgca gatagttgat taatttcatt ttgatatttc gtctggcgat
     2701 tttgaagtct tttttcaact ttatcttcga gattcttaaa gtctttgtat ttattttcag
     2761 gatggtcaac gtcaactttg ttagcgaata aagtagaaat tatttctttt acattatcgc
     2821 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     2881 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     2941 aaccagttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcac
     3001 tcctggcgtt ttcactacaa tattgggtga gactatcacc attaatacga attcctcttt
     3061 gaatgtggta gtcgtcctga tcgtagtaac cgagagataa gtaatcattt ccgtttttat
     3121 ttttaattac aatagcgaga gagtcgcctt ttttatcttt accgtaccag gtaaataagt
     3181 cagttggttc gcttttcccg ctctcatcaa attccgttac cgtatgtctg agtttgtgtg
     3241 atatcttatc gaagataaaa ccaggatctc catcatatat ttcttttaat gcgctgaaga
     3301 tcatttcctt gttattgata tcatgagtct tctcaactat gttgtcatta atgatagtac
     3361 ggacgagttc tttgtcttta tagttcgcag gtaattctat tggttcttgg ttgtcgatac
     3421 caatagtaat atggtctgta tcattggagc caatatcaat ataagtaact gaggaattat
     3481 taatattaga tctataagga tcgtcgttta ctcgtatggc gttttttata agctcaagta
     3541 gatgtttaat acaggtgctt attccttcct taccattaaa aaggtttttg aactcttgta
     3601 ctaatccatt aatttcatca ttgttatttt cattgatgga ttctgggtaa gctgcgctgg
     3661 tattgttgat tgaaatagat ggtagcatga ttttattcca tgttgattga tttgttacaa
     3721 atatatatta cctaatgaaa tagactatca aaaaacgctc atttatttag aacctatctt
     3781 taatttgagt gtataagcgc acaatagcca ggggggttga gtatcctgga aatgcagaaa
     3841 agtaaactct ttgagaagga cgagcagaac gcttgaaaat atgcatttcc cttcaggttt
     3901 gtctgtggat accacttact gatttgaaag ggcaagctgc catcaggctg tttgcaaaac
     3961 tcaggaggca agcaatgagg aaactacttt cggtgaagtg ggggagttaa agtcacctgc
     4021 atagcaggtg actttatcca ggcgaacgcg ttatcagaac gcgtagttaa agttagtacc
     4081 gaacagccag gctttacctt cagactcgaa ctggtatggg ccttcgttaa ttttcacgct
     4141 ctgaccgtgc atataagaaa caccaacgtc gactgaagca tctttattaa atgcgtaagt
     4201 cgtacctgca ctcagccaga aacggtcctg gtccggaatg gagatagaac gattctgagc
     4261 cggaactggg ctgtcatcaa aggcgatacc ggtacggaag gtccagttat catcgtagta
     4321 ataagtggta cccaacgcga tgcggtaagc atctttaaag ccttcatgtt tctggaacag
     4381 cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac tgactccagc tggtgtaagc
     4441 caggctatag tgaatcgccc actgtggatc aacacggtta taacctgaca cttcccacat
     4501 ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg ccacctgtcg cggtaggaat
     4561 tggcaaaccg tagttattaa acgcacgatt aagatcgctg ctgtagttac ctttgaagtc
     4621 aattttcact tcagaacggt aggtcagtgc atagcggtta tttttatcca gttcatacag
     4681 gattccggcg ttccagccaa agccccactg gttaccgttc agatgagcga ttttggtatt
     4741 actgtcgata ccgttggcgg tagctgccaa tgcttgccct tgaggagttt gcccggcagg
     4801 agattgcata atctgaccag caaccaactg ccccagatcg cctgcgaaac gttcaatttt
     4861 cgcgcgagcg tagatggcgt tgaaaccaag accaaagctc catgcattat ttaagcgata
     4921 cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt gtacccccga cagagccgcc
     4981 tgcataagta tcattaaact ctgttgccag accatagtta gaggtaatag aagcgcccca
     5041 accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc ggaacccatg ccgtaggcgc
     5101 gatgttatcg gctttcaggc tacgaccaga cggagacgtt ccgctgatat tcacatccgg
     5161 gtcaatataa accgcacccg cagaaaatgt cgggcggtca aacatggtaa tcaatgcggg
     5221 gttacggcta acgttacctg catcatcggc aattgcgcct tcccctgaat aagcccggcc
     5281 caggccagag gaagaaaatt cgtttaactg aaagcctgcc gaccaggcct gggtggagat
     5341 aagtgccact gcgactgcga gagcagactt tgtaaacagg gttttctggc tcatgaccat
     5401 aacctcaatg atttattttt atacaaacta tgttacgtgc tgtaacagga gcgcgaagtg
     5461 tagggtctga ggtacatctt agaaatcaga ccagtggcga gagtataggt cggaccagct
     5521 agaatgttgc aagtatgttt ccatcttttt tcaaatatga tctaagaaac gcgatccagc
     5581 tggcaaaatt tggaggttgc gcactcgcgg attcgggtga attttgtttt agatcatttt
     5641 taagtgtgat ttcggtcact tatccgattt tgcagaatta acaactggag cagcactttc
     5701 cggaagcgta aaatatccac aacgtttatc tggcaccttg ggtgcacata cagaggaaat
     5761 caacgatgag taaatgtagt gctgatgaaa ccccggtttg ctgctgtatg gatgttggca
     5821 ccattatgga caactccgac tgcaccgcgt cttacagccg cgtgtttgca aaccgcgcag
     5881 aagcagaaca aacgctggcg gcgctgactg aaaaatcccg tagcgtgaaa tccgaaccgt
     5941 gcaaaatctc cccgactttc actgaagagt ctgacggtgt gcgcctggat atcgatttca
     6001 cttttgcctg cgaagctgaa atgctgatct tccagctggg tctgcgttaa ttcctctcgg
     6061 gtacgtacgc ccctgtcagt tgctggcagg ggcgttttct tttctttctt ctgtcttcac
     6121 tgtgtcatag ctcccacttt cctttttctc cgcttggcta aatgtaaaaa aatggttaag
     6181 actatgatca ggtcagacca ctttatttat ttttttacag gggagtgtta tgggtcaggt
     6241 tttaccactg gttacccgcc agggcgatcg tatcgccatt gttagcggtt tacgtacgcc
     6301 ttttgcccgc caggcgacgg cttttcatgg cattcccacg gttgatttag ggaagatggt
     6361 ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa gtgattgaac agctggtctt
     6421 tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg cgtgaaattg ttctcggtac
     6481 cggaatgaat gtgcataccg atgcttacag cgtcagccgc gcttgcgcta ccagtttcca
     6541 agcggttgca aacgtcgcag aaagcctgat ggcgggaact atccgagcgg ggattgccgg
     6601 tggggcagat tcctcttccg tattgccaat tggcgtcagt aaaaaactgg cgtacgtgct
     6661 ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg aaactcttct ctcgcctgcg
     6721 tttgcgcgac ttaatgcccg taccgcctgc ggtagcagaa tattctactg gcttgcggat
     6781 gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc acccgagaac agcaagatgc
     6841 attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg tcagacggaa aactcaaaga
     6901 agaggtgatg actgccttta tccctcctta taaacaaccg cttgtcgaag acaacaatat
     6961 tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc ccggcgtttg atcgcaaaca
     7021 cggaacggta acggcggcaa acagtacgcc gctgaccgat ggcgcggcag cggtgatcct
     7081 gatgactgaa tcccgggcga aagaattagg gctggtgccg ctggggtatc tgcgcagcta
     7141 cgcatttact gcgattgatg tctggcagga catgttgctc ggtccagcct ggtcaacacc
     7201 gctggcgctg gaacgtgccg gtttgacgat gagcgaactg acattgatcg atatgcacga
     7261 agcctttgca gctcagacac tggcgaatat tcagttgctg ggtagtgaac gttttgctcg
     7321 tgaagtactg gggcgtgcac atgccactgg cgaagtggac gatagcaaat ttaacgtgct
     7381 tggcggttcg attgcttacg gacatccctt cgcggcgacc ggcgcgcgga tgattaccca
     7441 gacattgcat gaacttcgcc gtcgcggcgg tggatttggt ttagttaccg cctgtgctgc
     7501 cggtgggctt ggcgcggcaa tggttctgga ggcggaataa tggaaatggc atcagcgttt
     7561 actcttaatg ttcgtctgga caacattgct atcatcacca tcgacgtacc ggatgagaaa
     7621 atgaataccc tgaaggcgga atttgcctcg caggtgcgcg ccattattaa gcaactccgt
     7681 gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga aaccggacaa ttttatcgcc
     7741 ggagcagaca tcaacatgat tggcaactgc aaaacggcgc aagaagcgga agcactggcg
     7801 cggcagggcc aacagttgat ggcggagatt catgctttgc ccattccggt tatcgcggct
     7861 attcatggcg cttgcctggg cggtgggctg gagctggcgc tggcgtgcca cggtcgcgtt
     7921 tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag tacaacttgg attgttaccc
     7981 ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg tcagtacagc attagagatg
     8041 attctcaccg gaaaacaact tcgggcgaaa caggcattaa agctggggct ggtggatgac
     8101 gttgttccgc actccatttt gctggaagcc gctgttgagc tggcgaagaa ggatcgccca
     8161 tcttcccgcc ctctacctgt acgcgagcgt attctggcgg ggccgttagg tcgtgcgctg
     8221 ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc aaggcaatta tccggcgaca
     8281 gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg gcaccagcag cggctatgac
     8341 gctgaagctc gcgcctttgg cgaactggcg atgacgccgc aatcgcaagc tttgcgtagt
     8401 atcttttttg ccagtacgga cgtgaagaaa gatcccggta gtgatgcacc gcctgcgcca
     8461 ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg gcggtattgc ttatgtcact
     8521 gcttgtaaag cggggcttcc ggtcagaatt aaagatatca acccgcaggg cataaatcat
     8581 gcgctgaagt acagttggga tcagctggag ggcaaagttc gccgtcgtca tctcaaagcc
     8641 agcgaacgtg acaaacagct ggcattaatc tccggaacga cggactatcg cggctttgcc
     8701 catcgcgatc tgattattga agcggtgttt gaaaatctcg aattaaaaca acagatggtg
     8761 gcggaagtgg agcaaaattg cgccgctcat accatttttg cttcgaacac gtcatcttta
     8821 ccgattggtg atatcgccgc tcacgccgcg cgacctgagc aagttattgg cctgcatttc
     8881 ttcagtccgg tggaaaaaat gccgctggta gagattattc ctcatgcggg tacatcggcg
     8941 caaaccatcg ccactacggt aaaactggcg aaaaaacagg gcaaaacgcc cattgtcgtg
     9001 cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc cttacattaa tgaagctatc
     9061 cgcatgctga ccgaaggtga acgggtagag cacattgatg ccgcgctagt gaaatttggt
     9121 tttccggtag gcccaatcca acttttggat gaggtaggaa tcgacaccgg gactaaaatt
     9181 attcctgtac tggaagccgc ttatggagaa cgttttagcg cgcctgcaaa tgttgtttct
     9241 tcaattttga acgacgatcg caaaggcaga aaaaatggtc ggggtttcta tctttatggt
     9301 cagaaagggc gtaaaagcaa aaaacaggtc gatcctgcca tttacccgct cattggcgca
     9361 caagggcagg ggcgactctc cgcaccgcag gttgctgaac ggtgtgtgat gttgatgctg
     9421 aatgaagcag tacgttgtgt tgatgagcag gttatccgta gcgtgcgtga cggggatatt
     9481 ggtgcggtat ttggcattgg ttttccgcca tttctcggtg gaccgttccg ctatatcgat
     9541 tctctcggcg cgggcgaagt ggttgcaata atgcaacgac tggccacgca gtatggttcc
     9601 cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc gtggggaaag tttttggaaa
     9661 acaactgcaa ctgacctgca ataagaaggt caaagctata tgaatccgcg ctgaatggcg
     9721 gagtgttggt caaaatgtaa acgcatattg actatactta cgccattgag gtaaaaaaca
     9781 gcgtttcatt cggtgaatgg ataaggcaca atgccggcca ccgttttctt tctctggttt
     9841 cagatgaaag aaaacgggcg aatctggtta acaaaagcgg tgcaatatgc aagtttttat
     9901 catgcgtcac ggcgacgcag ccctcgattc cgccagtgat tcggttcgtc ctctgaccac
     9961 taatggttgt gacgaatctc gcctgatggc gaactggctg aaaggtcaaa aagtggaaat
    10021 cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa acactggaag aagtagggga
    10081 ttgtctgaac ctgccttcca gtgcagaagt tctgccggag ttaacgccct gcggcgatgt
    10141 tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa ggtgttgctt cggtactggt
    10201 gatctctcac ttaccgttag tcgggtatct ggttgccgag ttatgtccgg gcgaaacgcc
    10261 gcctatgttt accacctcgg ccattgccag cgtaacgctt gatgaaagcg gaaacggtac
    10321 attcaactgg cagatgagcc cgtgtaatct gaagatggca aaagctatct gatgtgttcc
    10381 aaattatgcc caatgaggta gattcattgg gcataattgc tcattaggca gcggatatct
    10441 gtcaacctca ctactatcga agactctcct tatctataaa taaaacactt tctacattca
    10501 ttcggaaatt attcttctac gcgtatcttg attttgccca aagttacatg ttgaaatgaa
    10561 taatatctgt ttgacactca ttgtc
//
LOCUS       DAJVQR010000123        10299 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVQR010000123 DAJVQR010000000
VERSION     DAJVQR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            48..236
                     /locus_tag="OQM52_003497"
     CDS             48..236
                     /locus_tag="OQM52_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1565205.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            211..441
                     /gene="ymcE"
                     /locus_tag="OQM52_003498"
     CDS             211..441
                     /gene="ymcE"
                     /locus_tag="OQM52_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCS1565206.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            431..604
                     /locus_tag="OQM52_003499"
     CDS             431..604
                     /locus_tag="OQM52_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS1565207.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(653..1726)
                     /locus_tag="OQM52_003500"
     CDS             complement(653..1726)
                     /locus_tag="OQM52_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1565208.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1828..4539)
                     /gene="torS"
                     /locus_tag="OQM52_003501"
                     /pseudo
     CDS             complement(1828..4539)
                     /gene="torS"
                     /locus_tag="OQM52_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4622..5650
                     /gene="torT"
                     /locus_tag="OQM52_003502"
     CDS             4622..5650
                     /gene="torT"
                     /locus_tag="OQM52_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1565209.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5623..6315)
                     /gene="torR"
                     /locus_tag="OQM52_003503"
     CDS             complement(5623..6315)
                     /gene="torR"
                     /locus_tag="OQM52_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1565210.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6402..7574
                     /gene="torC"
                     /locus_tag="OQM52_003504"
     CDS             6402..7574
                     /gene="torC"
                     /locus_tag="OQM52_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1565211.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7574..10114
                     /gene="torA"
                     /locus_tag="OQM52_003505"
                     /pseudo
     CDS             7574..10114
                     /gene="torA"
                     /locus_tag="OQM52_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10111..>10299
                     /gene="torD"
                     /locus_tag="OQM52_003506"
     CDS             10111..>10299
                     /gene="torD"
                     /locus_tag="OQM52_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1565212.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2473 a         2654 c         2752 g         2420 t
ORIGIN      
        1 acgtggcccc gcggcagcga acgctgttac gctctaaggt tgccgttatt actcaacatc
       61 tccatttccg ctgtccatgt tgtcatggtt cacagtaccg tacatcggca ttcgatgtga
      121 cggagcgaaa ccctttgggc gctaagtgta ttttttgtaa atcgactatg atcacctttg
      181 ataacgtcgc gctgcaaata cgcactgacc atgcgccgct ggatttcaca aaataatatc
      241 aggctccctc gtggagcctt ttttatatct gccttatttt tcttcaacgc tgtatgtata
      301 gtaagcgata acctgttgat tattgaatct ttcgggaaga tggcttataa catttcttac
      361 ctgaccaggg taccgggaac caacacctta ctggcgtgtt gctgtctttc aagaccagac
      421 gaggttaaca gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt
      481 tatcgcgcaa aaaatcgccg agctgaacaa gaatacaggg aaagaagtct ctgaaattcg
      541 cttcatcgca cgagaaaaaa tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat
      601 gtgattttgt gaacatcacc ccgtgcgagg tgatgttccg cttgttgcta atttagtgac
      661 caatcattgg cgcttgtgga attaagcgtc ggtacaattc ctccggcacc gggctttgcc
      721 atactcccgc atacattgcg taaccaatca ccgcaaacat aatccccaga accagtagcg
      781 tcattaacca gccagacaac gcaaaggctt ttttatttgc tgcaggtttt tgcagtgaaa
      841 aggtcaatgt tgaggctacc ggacatgact ctacgcaagt catacagccg gtacattcca
      901 ctgttcgtac ctgaattaat ttatctaccg ggatccgtga tgggcacttt tttgcgcatt
      961 tgccacaatc gatacaactt tcggcattgc gacgaatctt aaacggcgac aatagcgaaa
     1021 ccacgcccat cagcgcgcca tatgggcaaa gataacgaca ccaggcatgg cgaataaaca
     1081 ggctggcaat cagcaaaacg gtcacgctga ttaatgtcgc ggtccccata tgacgaaaga
     1141 aatcgagcat tttaacgtcc atcaccacgc tatagggcga caacataaaa tagtgaatcg
     1201 cctgagcggg catcaataac gcgatataga gaaataaact caacagcaaa tacttcacgc
     1261 cgcgcagagg aatatccagc cagcggggaa ggacacattg ccgaccaaac agtttgttac
     1321 cgagatcgcc gattaattca gaaagcgtac caaccgggca taaccatgag caaaaggcct
     1381 ttttgagtaa tagactgatg acgataaaag cgaccaataa cagcatcgcg gcggcgtgga
     1441 cggacggtaa ctgacctgtt gcaaggctat atttcagatt catcagaccg gcaatcggta
     1501 gccagccttc gatacctccc ggtctggcga caaatgtcgt actacctgcc gtttcgtaat
     1561 agcgcaccca ataccagaac gtgatggcaa tataaatatt cattgccaat agtaagaatt
     1621 gcgtcgcttt acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg
     1681 tcgtgcctgg ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgccagtcc
     1741 gttaagttgc ataccaaatg ccactattct agttattctt aactggctga tattgattca
     1801 aatcgcgttc aggtctttct tattcaatca tgcttccaga gcggcaacac tgcgtgtaat
     1861 ttcttcgtgt ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctgact
     1921 ggcgctacgc attcccagac tcgagcaact gctttttagc tgatgcgcgg cacgctttat
     1981 tttctcgctg tcctggctgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg
     2041 ttgtttaaat aatgccagcc attcgtggat cttctccgtc cccattaact gagcgtcttc
     2101 attgagttgc gagacatcca gtggtagatc gttattggct tgcagttgga gatagtgcgc
     2161 cagtaactga ccgagcactt cacgcggcac cggtttaggg ataatcccgc gaaatagtga
     2221 actggtacgc tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag
     2281 cgacggatat tgctgtgcca gttgccgggc aagggtaatg ccgtcgatat ccggcagatc
     2341 aaaatccacc agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc
     2401 ggcattgcca atagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg
     2461 ctgggttagc gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg
     2521 attggctgtt tttggcacgg gtgcagtggc aacacgtaac ggcaagcgta aacaaaaaca
     2581 gcttccaacc tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg
     2641 gctactgata gtcagcccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg
     2701 ctggaagatt tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac
     2761 cagccattgc tcgccatcag tgcgactacg caggataatg tgcccttcgt cagtaaaacg
     2821 cagggcattg ctcaataggt tggtaatgac ctgacgaata cgtcgtggat cgcccattaa
     2881 cgcgctcggt acatcatcgg caattgccgt tgccaggcga atcgggcgac ctttcacccg
     2941 tccgctcatt aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct
     3001 gactgaaaca tttttgccac ctgcttcgat agccgaataa tcgagaatat cgttgaggat
     3061 ggtcagcaac gattcgccag agtcggtaat cgcctgcaaa tcatcacgct gggcgttaag
     3121 tgcggggtta tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat
     3181 ctcatggctc atcgccgtca gaaacgccga ttttgcctgg ctggcttttt ctgcttccgc
     3241 ccgtgcctgt cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg
     3301 cgccgccagc tgttcgcggt ggcgattcag tgcatgaaca ctgctgcgaa acgcatccat
     3361 cagccgcccg atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat
     3421 atcaccgtcc agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgaatga
     3481 gcgataaacc acgcgccaga ggatcataat cagtgcgcaa agtgaaacca tccccagcaa
     3541 taacaggctg tattgcccgc gtgcactggc tttttccaga tgtgccagtc catgctgatt
     3601 gtgcagctca atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc
     3661 gatattattt tgggcgagag tttgcaggtg attgctgatt tcactgtcct gctggaacag
     3721 cgccaacaga tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac
     3781 tcccggatct tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg
     3841 cttttccagc gttggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg
     3901 ctgcacccgc agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat
     3961 atcaatcagc cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc
     4021 gtaaatcccg gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgctc
     4081 caggcgtgcg atctcatcgg cggcagctat ctgctgactg agttgccgtt gttgctggcg
     4141 tagttgcaga cgccgcccca ccagttcccc ttgctgacgt aatgaacggg agatctcctg
     4201 ctcctgttgt tcaatagcgg tggtatcaaa accttgttcc cgtaacgctt gcagcaacgc
     4261 attaatcttc aggctttatg cggtgagcat tcgcccctgc gcctgccaca tcttttcgtt
     4321 atcggcactg gtcaggttct gcgcggcgaa aagttcccag gcgctggctt cgctcaactg
     4381 gcgcgccata ttcatggtag gaatcaatgc ctgagtattg tctttttcca cctggctgat
     4441 aaagcgcagg ttgtaccatc ccaccagggt actggtcagg gttaacagcg ccatcagggc
     4501 aaagcccatc cagagtcttc gggtcagggt taaattcacg gtcggtgcac ttaaggtgaa
     4561 aaaggttgag tcgcaaagtg gaatgcatct agcataaagc cttattattg atgaggctat
     4621 catgcgcgta ctgctatttt tacttctttc ccttttcatg ttgtcggcat tttcggctga
     4681 taacctgttg cgctggcatg atgcgcagca tttcacggtg caagcctcta tgccgcttaa
     4741 agccaaacgc gtatggaaac tgtgcgcgct ttatcccagc ctgaaagatt catactggtt
     4801 atcgttgaac tatggtatgc aggaggctgc tcgccgctac ggtgtggatt taaaagtgct
     4861 ggaggcaggc ggctacagcc agttggctac ccagcaagca caaatcgacc agtgtaaaca
     4921 gtggggcgca gaggccattt tgctcggtag tagcacgacg tcatttcccg acctgcaaaa
     4981 gcaggtagca aatctgccgg tgatcgaact ggtaaatgct cttgatgatc cccaggtgaa
     5041 aagccgcgtt ggtgtaccct ggtttcagat gggctatcaa cctgggcgat atttggtgca
     5101 atgggcgcac ggtaaaccac tgaacgtgct gctgatgccc ggacccgata acgccggggg
     5161 cagtaaggag atggtcgagg gttttcgcgc agccattgcc ggaagcccgg tgcgtattgt
     5221 tgatattgcg cttggtgata acgatattga aatccagcgc aacctgttgc aggagatgct
     5281 ggaacgccac ccagaaatcg acgtcgttgc cggaacggcc attgcggcag aggcggcaat
     5341 gggtgaaggg cgtaacctga aaacgccgct taccgtggtg tcgttttatc tttcacatca
     5401 ggtgtatcgc gggctgaagc ggggaagagt gattatggct gccagcgatc aaatggtctg
     5461 gcagggggaa ctggcagttg agcaggccat caggcaatta caggggcaat cggtgtctga
     5521 taatgtcagc ccaccgattt tagttctgac gccgaaaaat gccgaccgag aacatatccg
     5581 ccgctcgctg tcaccggggg gatttcgtcc ggtctatttt tatcagcaca catcagcggc
     5641 taagaaataa ccttcaccat gttgcgtcac cagtaaatcc gcgctgagtt tatgacgtaa
     5701 acgacgaatt aacacatcga cggtgcgcag gtcagggttt tccacccgac gcgcagaaag
     5761 catacgcagc agacgctcac ggctgagaat ttcgcccgga ttagtcacaa atgccaccaa
     5821 catttcatac tctgcgcggg tcagtttaat cggctcgcca tcccgctcca gcgtatggcg
     5881 cgacacattc aggcaataac ccgcaaagcg atagcagtag tcctgagtgt gcggttgagc
     5941 ttgtcgcgcg aggtcgattc gccagagcag atttttcacc cgtactacca gttcgcgcag
     6001 ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc agcccaacaa tacggtcaat
     6061 ccgatcgctg cgtccggtaa ccagaataat ccccaccgtt gagcgttctc gcagggcgcg
     6121 ggttaacatc aggccatttt catcgggtaa gttgatatcc agcagaatta aatctaccgg
     6181 ctgattctgc ataatttccc gtagcccggc accgctcgcc gtaacggaaa cggtataccc
     6241 ctcctgagtg aagtaggatt gtaatcgcgc ctgggtaacc ggcttatctt caacaataac
     6301 aatgtgatgt ggcatcagag ggttttactc attctgttca tatctgttca tatttgctca
     6361 ttaagatcgc ttcactaaac cataattcta caggggttat tatgcggaaa ctctggaacg
     6421 cgctacgccg acccagtgct cgttggtcgg tactggcgct ggtcgcaatt gggattgtga
     6481 ttggcattgc gctgattgta ttgccacacg ttgggatcaa agtcaccagc acaaccgaat
     6541 tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga atataaacag tcggtgcatt
     6601 tccagaacgc ctccggcgtg cgagctgaat gccatgactg tcatatcccg ccggatattc
     6661 caggcatggt gaagcgcaaa ctggaagcga gcaacgacat ttaccagacc tttattgccc
     6721 actccattga tacacctgaa aaattcgaag ccaaacgcgc ggaacttgcc gagcgtgaat
     6781 gggcgcgaat gaaagaaaac aactcggcaa cctgtcgctc ctgccataac tacgatgcga
     6841 tggatcatgc caggcagcat cctgaagcgg cacgtcagat gaaggtggca gcgaaagata
     6901 atcaatcctg catcgactgt cataaaggta ttgcccacca gttaccggat atgagtagcg
     6961 gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa tgacagtggt gacacgctgt
     7021 actctattga tattaagcca atttatgcgg cgaaaggcga taaagaagcc tctggttctc
     7081 tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg cgactggctg caaattgaaa
     7141 ttaccggctg gacggaaagc gccggacgtc agcgtgtact cacccaattc ccaggtaaac
     7201 gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca ggtgaaaacg ctggagaaaa
     7261 ccaccgttgc cgacaccaat accgagtgga gcaagttgca ggccaccgcg tggatgaaga
     7321 aaggcgacat ggtgaacgat atcaaaccga tctgggccta tgcggattcg ttgtacaacg
     7381 gcacctgtaa cgagtgccac ggcgcaccgg aaatcgccca ctttgacgct aacggttgga
     7441 tcggcacgct caacggcatg attggcttta ccagtctcga taaacgtgaa gaacgcactt
     7501 tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg taaggctcac ggcgataaga
     7561 aggaagaaaa ataatgaaca ataacgatct ctttcaggca tcacgtcggc gttttctggc
     7621 acaactcggc ggcttaaccg tcgccgggat gctggggccg tcattgttaa cgccgcgccg
     7681 tgcaactgcg gcgcaagcgg cgactgaggc tgtcatctcg aaagagggca ttcttaccgg
     7741 gtcgcactgg ggggctatcc gcgcgatggt gaaggatggt cgctttgtgg cggcaaaacc
     7801 gttcgaactg gataaatatc cgtcgaaaat gatcgccgga ttgccggatc acgtacacaa
     7861 cgcggcacgt attcgttatc cgatggtacg cgtggactgg ctgcgtaagc gccatctgag
     7921 cgacacctcc cagcgcggtg ataaccgttt tgtgcgcgtg agctgggatg aagccctcga
     7981 catgttctat gaagaactgg aacgcgtaca gaaaactcac gggccgagtg ccttgctgac
     8041 cgccagtggt tggcaatcga cggggatgtt ccataacgct tcggggatgc tggcgaaagc
     8101 tattgccttg catggtaata gcgttggtac gggcggagat tactctaccg gtgctgcgca
     8161 ggtgatcctg ccgcgcgtag tcggttcaat ggaagtgtat gaacagcaaa cctcctggcc
     8221 gctggtattg cagaacagca aaaccattgt gctgtggggc tccgatttgc tgaaaaacca
     8281 gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa tattacgagc agctaaaagc
     8341 gaaagtcgcc gccggtgaag ttgaggtcat cagcatcgat ccggttgtca catccaccca
     8401 tgagtatctg gggcgcgagc atgtgaagca cattgcggtt aacccgcaaa ctgacgtgcc
     8461 gctgcaactg gcgctggcac atacgctgta cagtgaaaac ctgtacgaca aaaacttcct
     8521 cgctaactac tgtgtgggtt ttgagcagtt cctgccgtat ctgctgggtg agaaagacgg
     8581 tcagccgaaa gatgccgcat gggctgaaaa actgaccggc attgatgccg aaaccattcg
     8641 tgggctggcg cggcagatgg cggcgaacag aacgcaaatt attgctggct ggtgcgtgca
     8701 gcgtatgcag cacggtgaac agtgggcgtg gatgattgtg gttctggcgg cgatgctggg
     8761 gcaaattggc ctgccaggtg gtggttttgg ctggcactac aacggcgcag gcacgccggg
     8821 gcgtaaaggc gttattctga gtggtttctc cggctctacg tcgattccgc ctgttcacga
     8881 caacagtgac tacaaaggct acagcagcac tattccgatt gcccgtttta tcgatgcgat
     8941 cctcgaaccg gggaaagtga tcaactggaa cggtaaatcg gtaaaactgc cgccgctgaa
     9001 aatgtgtatt tttgccggaa ctaacccatt ccatcgccat cagcagatca accgcattat
     9061 tgaaggctgg cgcaagctgg aaacggttat cgccatagat aaccagtgga cctcaacctg
     9121 ccgctttgcc gatatcgtgc tgcctgcgac cacgcagttt gagcgtaacg atctcgacca
     9181 gtacggcaac cactccaacc gtggcattat cgccatgaaa caggtggtgc cgccgtagtt
     9241 cgaggcgcgc aacgactttg atattttccg cgagctgtgc cgtcgcttta atcgtgaaga
     9301 agcctttacc gaagggctgg acgaaatggg ctggctgaaa cgcatctggc aggaaggtgt
     9361 acaacaaggc aaaggacgcg gcgttcatct gccagcgttt gatgacttct ggaataacaa
     9421 agagtacgtc gagtttgacc atccgcagat gtttgttcgc caccaggcat tccgcgaaga
     9481 tccggatctc gaaccgctgg gcacgccgag tggcctgatt gagatctact cgaaaaccat
     9541 cgccgatatg aactacgacg attgtcaggg gcatccgatg tggtttgaga aaatcgaacg
     9601 ctcccacggt gggcccggct cgcagaagta tccgttgcat ctgcaatctg tgcatccgga
     9661 tttccgactt cactcgcagc tatgtgagtc ggaaacgctg cgtcagcaat atacggtagc
     9721 gggtaaagag ccagtgttca ttaacccgca ggatgccagc gcgcgcggta ttcgtaacgg
     9781 tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg gcaggagcag tagtttctga
     9841 ccgctatgca cccggcgtgg cgcgaattca cgaaggggca tggtacgatc cagataaagg
     9901 cggcgagcct ggtgcgctgt gcaaatacgg taaccccaac gtgctgacca tcgacatcgg
     9961 gacttcgcag ctggcgcagg cgaccagtgc gcacactacg ctggtggaaa ttgagaagta
    10021 caatggaaca gtggagcagg tgacggcgtt taacggcccc gtggagatgg tggcgcagtg
    10081 cgaatatgtt cccgcgtcgc aggtgaaatc atgaccacgc tgacagcaca acagattgcc
    10141 tgtgtttacg cctggctggc gcagttgttc tcccgtgaac tggacgatga acaactgacg
    10201 caaatcgcca gtgcgcagat ggctgaatgg ttttcgttgc tgaaaagcga accgccgctc
    10261 actgcggcgg tgaatgatct ggaaaacagt attgccacg
//
LOCUS       DAJVQR010000124        10105 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVQR010000124 DAJVQR010000000
VERSION     DAJVQR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            123..254
                     /locus_tag="OQM52_003507"
     CDS             123..254
                     /locus_tag="OQM52_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1565213.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="OQM52_003508"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="OQM52_003508"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1565214.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="OQM52_003509"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="OQM52_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="OQM52_003510"
     CDS             2687..3739
                     /locus_tag="OQM52_003510"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCS1565215.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRVS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="OQM52_003511"
     CDS             3815..5248
                     /locus_tag="OQM52_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCS1565216.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="OQM52_003512"
     CDS             5431..7611
                     /locus_tag="OQM52_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1565217.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="OQM52_003513"
     CDS             complement(7752..9035)
                     /locus_tag="OQM52_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1565218.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..10051)
                     /locus_tag="OQM52_003514"
     CDS             complement(9170..10051)
                     /locus_tag="OQM52_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1565219.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2452 a         2357 c         2552 g         2744 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgtatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggtagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     9601 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     9661 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     9721 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcagctattg
     9781 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc gccttgccct
     9841 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggag
     9901 catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     9961 gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
    10021 cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
    10081 caatgttggc ctgtatagct tcagt
//
LOCUS       DAJVQR010000125        10045 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVQR010000125 DAJVQR010000000
VERSION     DAJVQR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..235)
                     /locus_tag="OQM52_003515"
     CDS             complement(<1..235)
                     /locus_tag="OQM52_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565220.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQ"
     gene            667..1785
                     /locus_tag="OQM52_003516"
     CDS             667..1785
                     /locus_tag="OQM52_003516"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1565221.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1851..2099
                     /locus_tag="OQM52_003517"
     CDS             1851..2099
                     /locus_tag="OQM52_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1565222.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2164..2532
                     /locus_tag="OQM52_003518"
     CDS             2164..2532
                     /locus_tag="OQM52_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1565223.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2626..3279
                     /gene="nfsB"
                     /locus_tag="OQM52_003519"
     CDS             2626..3279
                     /gene="nfsB"
                     /locus_tag="OQM52_003519"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1565224.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3387..4634
                     /gene="ybdG"
                     /locus_tag="OQM52_003520"
     CDS             3387..4634
                     /gene="ybdG"
                     /locus_tag="OQM52_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1565225.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4702..6078)
                     /gene="pheP"
                     /locus_tag="OQM52_003521"
     CDS             complement(4702..6078)
                     /gene="pheP"
                     /locus_tag="OQM52_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1565226.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6179..9322)
                     /gene="cusA"
                     /locus_tag="OQM52_003522"
     CDS             complement(6179..9322)
                     /gene="cusA"
                     /locus_tag="OQM52_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS1565227.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9334..9852)
                     /gene="cusB"
                     /locus_tag="OQM52_003523"
                     /pseudo
     CDS             complement(9334..9852)
                     /gene="cusB"
                     /locus_tag="OQM52_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2460 a         2772 c         2432 g         2381 t
ORIGIN      
        1 gctgctcgat cacagctttc acgaccttaa tacgcaactc tggatcagca ctaacgcctt
       61 taggtttggg aattaaacct ttttctccat gtttttcata gagggcaacc catgtcctga
      121 cctgggttcg ggggacacca aaacgtgccg agatgatcct gtaaccatca tcagtagtga
      181 agtagtgatt cacgacttca aggcgctttt caaaagggta ttttggcttt gacatattag
      241 gggctattcc atttcatcgt ccaacaaaat gggtgcagta cagggttgat agaaatatct
      301 cctggggctt catctttcca gacctcagag taaacctgaa atcggaaagc ttcaggcacc
      361 cgcagttatc ttacccttcc tcaacaaaat agctgtttat ttatacagca aatcctgttt
      421 cggacttctc actccccatt tgatcaactg aagctgaacc cgtttttttc tgcatgcctc
      481 aatcgtcaaa attcttcgcc accagccagc tctgccataa cttgccacat tgaaaaagta
      541 gccaaagtgc ttcaggcacc atcagttcac catcaccaaa aaaaagaaac attccagaac
      601 ttccgcatct cgcccagccg ctccgacttc tatactgaat agaaaacgtc aacttaagag
      661 aaacctatgc cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg
      721 gaaatgcagg tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt
      781 gacgcggtta aaaataagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg
      841 ctggagctgg cgacggatgt ttgccgtgat atcaaccagg ctgccgggca gttttcagcg
      901 atgcagaaag tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc
      961 acgcccccgt ttcagaaatg gcagcgtcag gaggtatgcg acaacgaacg ctatcaacga
     1021 acgctggaaa actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat
     1081 gttggctgtg ccagtggcga tgacgccatt tatttgctgc acggcttgtc acggtttgtg
     1141 ccgcacttta ttgccctttc cgccgcgtcg ccatatatgc agggaacgga tacgcgtttt
     1201 gccccctcac gaccgaatat tttttccgcc tttcctgata atggcccgat gccgtgggtc
     1261 agtaactggc aacaatttga agccctgttt cgctgtctga gttacaccac gatgatcgac
     1321 agcattaaag atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt
     1381 cgggtgatgg ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag
     1441 gccaccgccc actggttact gacggaacgc ccgttcaaac atcaggagaa agattacctg
     1501 ctgtataaat tcaaccgttt ccaggcctgt cgctatggtc tggaaggcgt cattaccgat
     1561 ccgcacactg gcgatcgtcg accgctaacg gaagatacct tgcgattgct ggaaaaaatc
     1621 gccccttcag cacataaaat tggcgcatcg agcgcaattg aagcactgca tcgccaggtc
     1681 gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg cggctcgctg
     1741 attgggctgg tgaaaaagca ttgtgagatc tgggccggtg actaagccgg gaattgcctt
     1801 acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc
     1861 ctttagaaac cttgaccacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc
     1921 tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc
     1981 tgatggatct tagccagctt tacactttat tgttttgtct gtggttttta gtgctgggcg
     2041 ctattgagta ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa
     2101 cgtcgattat cgccgaactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg
     2161 tttatggata agcaatcact gcacgaaacg gcgaaacgcc tagctcttga gttacccttt
     2221 gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaagatt
     2281 tttatgctgt cgtcggagct acgcggcgtc ccctttatca atctgaagtc cgatccacaa
     2341 aaatccctgt taaatcagca aatataccca agcattaagc cagggtatca catgaataaa
     2401 aagcactgga tttcggtgta ttccggcgag gaaatctccg aagcgttact tcgcgatctg
     2461 atcaacgatt cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg
     2521 cgtccaggct aaagcgggaa tctatagcgc atttttctcg cttaccattt ctcgttgaac
     2581 cttgtaatct gctggcacgc aaaattactt acacatggag tctttatgga tatcatttct
     2641 gtcgccttaa agcgtcattc cactaaggca tttgatgcca gcaaaaaact taccccggaa
     2701 caggccgagc agatcaaaac gctactgcaa tacagcccat ccagcaccaa ctcccagccg
     2761 tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc
     2821 ggtaattatg tgttcaacaa acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt
     2881 gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga agatgccgat
     2941 ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgcc
     3001 tatatgcacc gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc
     3061 aacgtcggta acttcctgct cggcgtggcg gcgctgggtc tggacgcggt gcccatcgaa
     3121 ggttttgacg ccgcaatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt
     3181 ctggtggttg ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa
     3241 tctcgtctgc cgcaaaacac caccttaacc gaagtgtaat tctctcttgc cgggcatctg
     3301 cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata
     3361 ggctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag
     3421 cgggtattga gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga
     3481 ccgccgtcgt ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg
     3541 ccatcgccag ttcacggctt tggttgcaaa tcattaccca gaataaactc ttccaccgtt
     3601 tagcttttac cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag
     3661 gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc
     3721 tgctttcagt cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa ttcccggcgg
     3781 catctcagtt accgctgaaa gggatatttc aggggattaa actgatcggc gcaatcctgg
     3841 tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattcta atcagcggtc
     3901 ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga tccgattctt ggtctggtgg
     3961 caggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg gaaatgccga
     4021 aatacggcgc ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact
     4081 gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact
     4141 ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca
     4201 ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg catttgttaa
     4261 agccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta
     4321 cggagtcggt attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga
     4381 acgaatatct gcgtaaccat ccgcggattc gtaaagatat gaccttaatg gtacgccaac
     4441 tggctccagg tgataacggt ttaccgctcg agatctacgc gtttactaac accgtggtgt
     4501 ggctggaata tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag
     4561 agtttggtct gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg
     4621 catttaagca gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt
     4681 gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa
     4741 tacaatccac accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag
     4801 cagcaaaatc atgccgagga aggcgatgca aagatagttg ccgaacggat agagcagcgc
     4861 cttaaactgt gtttcacgcc cctgacgtcg catcgccgcg cgaaaacgca gatgcgccag
     4921 gcagatcata atccagttca acagcaacgt tgctaccacc agcgccatca gcagaccaaa
     4981 cgctttttgc ggcagcagat agttgattaa caccaccagc gaagtgatcg ctccggaaag
     5041 catcagcgag ttaatcggca caccgcgacg gctgacgcga gtcaaaaact tcggcgcatt
     5101 accctgcaca gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac
     5161 cgacagcgat gccactagaa tgacgaagtt cagcgcagaa gctaccacat tgctgtcgag
     5221 attatggaaa atcatcacaa acgggctact gttggatttc acttccaccc acggatagag
     5281 tgccagtaaa accaccagtg aaccgatata aaacagcagg atgcgataca ccacctgatt
     5341 taccgctttt ggaatgcttt tttccggatc gcgcgcttca gcggctgtaa tcccaatcag
     5401 ctccagaccg ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccagcc
     5461 ggtggcgaag aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgta
     5521 accagaaaac agcagccaca ggccaaagcc gatcataccg atgatcgcca gcactttaat
     5581 cagcgcaaac cagaactcgg tttcgccata taagcgcacg ttcaccaggt taacggcgtt
     5641 gatgataata aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg
     5701 catatagatg cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta
     5761 gttccagcca gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa
     5821 tgaaccggat accggctctt cgaccaccat ctcgccaagc tggcgcataa tcaggaaagc
     5881 gatgatcccg gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg
     5941 gccaatgcca agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg
     6001 acggttatgt aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac
     6061 ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta
     6121 acatttagtg cccatcgtcg caagacacaa tccacacggt taaacgaggt atcctgcttt
     6181 aattccgtac ccgatgtcgg tgcagccaca tcagcttata cgccgccggg ataataaaca
     6241 gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg tgcggcaatc cggctcatca
     6301 cctctgaacc agcccccgtt ccccacagaa tcggcagcag accggcgata atcaccgcca
     6361 ccgtcatcgc tttcgggcgc acgcgcagga ccgcgccgtg atacaacgcc tcatccagct
     6421 tctgctcgct gaatgtttgt ggattattca acgacggctc ggcctctatg gcgtgacgta
     6481 aatacatcag catcaccacg ccaaattcgg cggcgacccc ggcaagggca ataaagccgg
     6541 tacccgtcgc cacagaaaga tgaaagccca tccaccacag taaccagatg ccgcccacca
     6601 gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc cacccgacgg aatgccagat
     6661 acaacagcac gaagatgatc atcagtgtca tcggcaccat gagtttcagc ttatggttgg
     6721 cgcgctccag tagctcgaac tgcccggaga atgccacgct ggtgcccggt tttaactgca
     6781 ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga caccatgtca cgatcgcggg
     6841 catcgatata aatccagctc gtcgggcgcg cattctcggt tttcagcatc gacggtccgg
     6901 tagagacttt aacgtcggcc acgtctgcca gggtgatttg ctgcttcatc ggcgtcagga
     6961 tcggcaactg gcgcagtgcc tgcggactat cgcgccagct ttgcggataa cgcagattaa
     7021 ttggataacg ggcaatccct tccaccgttt cgccaaccat cgccccgccc accgcagaag
     7081 tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg tgcggctttt tcacggttaa
     7141 tctcaacgtt gatatagcgc ccaccttcca gccgctcagc aagcgcagaa gctacgcctg
     7201 gcaccgttcg cgccacttct tcaatttgct cagccatcgc gtcgatatcc gccagcacag
     7261 tgccggaaac tttaatgccg atggggcttt taatgccggt tgagagcata tcgatacggt
     7321 tacgaattgg cggcacccac agattcgcca gccccggcag acgcacggtg ttatccagtt
     7381 cctcaatgat tttgtccatt gtcatgcctg gccgccactg atcctgtggc ttaagctgga
     7441 tggtcgtttc taccatctcc agcggagcag aatcggtggc ggtttccgct ttcccggttt
     7501 tgccaaatac ccgcgccact tcaggtacgc tcataattag cttgtcggtt ttttgcagca
     7561 tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga tggcatatac aacaagtcgc
     7621 cttcattgat ctgcggtaaa aattccccgc caactttatt gagcggccag agaaccgtca
     7681 gcaccgaaag cgccgccacc agcagcgtgg ttttcggcca gtgcagtact ttcagcaaca
     7741 gcggatgata aacacgaatc aaaaagcgat tgagcggatt actgctttcc ggcggaattt
     7801 tgccacggat ccagtaaccc atcaggatag ggatcactac gatcgccagc agcgccgcac
     7861 ccgccatcgc atacgttttg gtgaacgcca acgggccaaa cagacgccct tcctgccctt
     7921 ccagggtgaa gatcgggata aacgacaacg tgataatcag cagactgata aacagcgccg
     7981 gccccacttc aacagacgca tcggtgatca cctgccagcg cgttttatta tccagcgtgg
     8041 cgtcaggatg ctggtgctgc cactcttcca gctgtttatg cgcattctcg atcatgacga
     8101 tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc acccagcgac ataatattgg
     8161 cattcagccc ctggaagggc atgacaataa aagcaataca caaccccagc ggcaacgaaa
     8221 taatcgccac cagcgccgag cgcacatgcc agagaaacgg cgcgcagact accgccacca
     8281 caataaactc ttccagcaac ttgccgctga ggttgtcgat ggcccggtca atgagctggc
     8341 tgcggtcgta tgttgtaact atctccacgc cttccggcag actacttttc agcgtttcca
     8401 gtttgtcctt cacggcggcg atcacttctc gggcgttttt gccggatcgc aggatcacca
     8461 ccccgcccgc cacttcgcct tcgccgttta gttcggcaat gccccggcgc atcttcgggc
     8521 caatctggac cttcgcaaca tcgcgcagat aaacgggcac gccattttca ctggctttta
     8581 aaacgatgtg attaaagtcg tcgagcgttt gtagatagcc gctggcgcgc accatatatt
     8641 ctgcttccgc cagttcgatt gacgaaccgc ccgcttcctg gtttgaagca tccagcgcgc
     8701 tttttacttc ggcgagactg atgccatact gcgccaggcg ctgaggatcg ataaccacct
     8761 gatactcttt caccacaccg cctaccgacg ccacttccgc aacgtcagga atggttttca
     8821 gctcatattt gagaaaccag tcctgcaatg agcgtaaatc ggcaagatcg tgcttaccgc
     8881 tgcgatccac cagtgcatat tcatagatcc agccaacacc cgtggcatct ggtcccagct
     8941 cggcgctgac tcccgcaggc agcttaccct gtacctggtt gaggtactcc agcacccgcg
     9001 aacgcgccca gtacggatcg gtgccatctt cgaaaatgac atacacataa gagtcgccaa
     9061 actgcgagaa accacgcaca gtcttcgcgc caggcaccga caacatggtg gtggttagcg
     9121 gataagtcac ctgattttca acgatttgcg gtgcctggcc gggatagctg gttttaataa
     9181 tcacctgcac atcggagaga tccggcaacg catccactgg cgtattaatg atggtccagg
     9241 tgccccagat gctcagaaac aacgcgccca tcagcaccaa aaaacggttc gccaccgagc
     9301 gacgaataat ccattcaatc attggttatt ccctcaatgc gcatgggtag cactttcaga
     9361 gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa tcaatcagga acaggccgcc
     9421 ggaaaccacc ttttcacctt ccgccagacc agagcgtaat gcggtgacgc cttgcgatgc
     9481 ctggaaaaca gcaacgcgtt tcggtacaaa gcgcccgtcg gcatcaacgg taatcacccg
     9541 ctgttcgttg ccggtatcaa tcagcgcctg tgacggaatg agcagcatcg gttcgctggc
     9601 ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc gcctcgtcgg cgttgtcgac
     9661 ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg ccaggtagca gcgtccattt
     9721 gcggatggtg agtgttttat ccggtcgcgc cggaacggtg agggtaaact gcgaggcatc
     9781 tttcaccagc caggcgatgg actccgggat cgcagcagtg acccacaccg ggtccatacc
     9841 ctgaattcgc gattacgcgc aatccccctg agaaccgcca cgaacaaggg tttcatgatt
     9901 tgtgatttta gtttggtacg caatttggta cacaacacta ttttcacttc agcgggtagt
     9961 catcaaactc accataacga gcatcgttga tgatgtatgt gatcaccaca aatatcacat
    10021 ctggactagt tccgtcttca tgcgc
//
LOCUS       DAJVQR010000126         9928 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVQR010000126 DAJVQR010000000
VERSION     DAJVQR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..1024)
                     /gene="bglB"
                     /locus_tag="OQM52_003524"
     CDS             complement(<1..1024)
                     /gene="bglB"
                     /locus_tag="OQM52_003524"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCS1565228.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVEFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1043..2920)
                     /gene="bglF"
                     /locus_tag="OQM52_003525"
     CDS             complement(1043..2920)
                     /gene="bglF"
                     /locus_tag="OQM52_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS1565229.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3054..3875)
                     /gene="bglG"
                     /locus_tag="OQM52_003526"
     CDS             complement(3054..3875)
                     /gene="bglG"
                     /locus_tag="OQM52_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS1565230.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4162..4887)
                     /gene="phoU"
                     /locus_tag="OQM52_003527"
     CDS             complement(4162..4887)
                     /gene="phoU"
                     /locus_tag="OQM52_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1565231.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4902..5675)
                     /gene="pstB"
                     /locus_tag="OQM52_003528"
     CDS             complement(4902..5675)
                     /gene="pstB"
                     /locus_tag="OQM52_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1565232.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5767..6657)
                     /gene="pstA"
                     /locus_tag="OQM52_003529"
     CDS             complement(5767..6657)
                     /gene="pstA"
                     /locus_tag="OQM52_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1565233.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6657..7616)
                     /gene="pstC"
                     /locus_tag="OQM52_003530"
     CDS             complement(6657..7616)
                     /gene="pstC"
                     /locus_tag="OQM52_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1565234.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7703..8743)
                     /gene="pstS"
                     /locus_tag="OQM52_003531"
     CDS             complement(7703..8743)
                     /gene="pstS"
                     /locus_tag="OQM52_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1565235.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8991..>9928)
                     /locus_tag="OQM52_003532"
     CDS             complement(8991..>9928)
                     /locus_tag="OQM52_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1565236.1"
                     /translation="ITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSK
                     INQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNR
                     IEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIR
                     GDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNT
                     DDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEK
                     SGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2560 a         2583 c         2293 g         2492 t
ORIGIN      
        1 atccaaccgg atcaatttgc cacccccact ctgaactttt cagatgcgga ttggggatca
       61 tattcagtat gttgccctgc gcatttttat taatgctttc gtcgtgggaa acacaaccag
      121 tcatgtaata actaaaagag atgaattcta cagtatgttt taaatcttct gcgtcacttt
      181 cagtcatctc aatggtgata ttgtggtcgc ggaagaaacg ctgcatatag ccgggatatt
      241 ggccacgcgc ctgaacatca ccaaagaaca tccagcgccg gttctcttcc atggcctgca
      301 acatatcctg tggctggcag gtgagggggt aaaccagccc accgagaagc atattgccga
      361 ttttcgcttc ggggatcagg ctatgatagg ctttaactgc ccgcgcactg gcaaccagtt
      421 gatgatggat cgcctgataa acttccgcct cgccactctc ttctgccagc cccacgcccg
      481 tgaatggcgc gtgtaacgac atattgattt cattaaacgt cagccataac gctactttat
      541 gttggtagcg ggtaaagacc gtgcgggcgt aatgctcgaa gtgatcgatg accgcccgat
      601 tagcccaacc gccgtagttt ttcaccagcc catatagcat ttcgtaatgg gataacgtta
      661 ccagcggctt gatccccgcc tgcgccattt catcaaacag ccgatcgtaa aacgctaacc
      721 ccgcttcatt cggttcggct tcgtcgccct gagggaaaat tcgcgcccag gcaatggaaa
      781 tacgcagaca ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtaat
      841 aaaaatcgat ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc
      901 ccattacgcc atgaggctgt aaatctgagg tcgtgatccc tttgccatct tccagccagg
      961 caccttccac ctgattggca gctgttgcgc caccccaaag aaatgtttct ggaaatgctt
     1021 tcataattaa ctccttttat cgttagcgaa tgatggataa cagcggttca cctgcgctta
     1081 tctgcgccgt gccgtggggt aatacgtctg taaaatcatc gctattactg attaataccg
     1141 gcgtcgtcag atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat
     1201 cgcctgtatt gaccttgtca cccacgttga cgtgagcgga aaagaatttg ccgtccagtt
     1261 ttacggtgtc gataccgaca tgaatcagga tctccacacc atcatctgac tcaatgccaa
     1321 tggcgtgtaa tgtggcgaac aacgaagcaa ttcgacccgc aaccggagaa cgcacttcac
     1381 caaccgaggg cagaatggcg atacctttac ccaacaggcc actggcaaac gtggtatcag
     1441 cgacgtgaat gagcggcaca atctctcccg tcatcggtga acagataccg ccctgctcag
     1501 gtggtgtaat aacctctggt gttttctctt gcggggcacc ctgcgctggc tgacgtttac
     1561 tggtgatgaa atgaagcatc aacgtaccaa caaatgcgca acctatggca atgacaccgc
     1621 caataacgct ggcccagacg gtgaaatcaa tccccgttga cgggatggtc tgcatgaagg
     1681 tgaaaatact tggcaaacca aaggagtaaa ctttcgtttg cgcgtagcca atgatggtgg
     1741 cccccaatgc tccactgata caggcgataa caaaggggta cttacgcggc aggttgacgc
     1801 catataccgc tggttcggtg ataccaaaca gacccgtcaa cgccgctgat cccgccacca
     1861 cttttttctg cgcatcgcgt tcgcagagga agacgccgag cgccgccccg acctgcgcca
     1921 taatggcggg cattaacagc gggatcatgg tgtcgtagcc cagcacggtg aagttattga
     1981 tacacagcgg caccaggccc ctgtgcagtc cgaacatgac gaagatttgc cagaagccgc
     2041 ccattaccgc gcccgcaaat gcaggaaccg cctgataaag ccagagataa ccggcggcaa
     2101 tcatttcgct tatccaggtt gatagctgcc ccaccagcag aaaggtgacg ggtgtgataa
     2161 ccatcagaca tagcaatggt gtgaagaaat ttttgattgc cgacggtaac cacgcattaa
     2221 gtcggcgttc cagaatgctg cacaaccagg cagaaaaaat aatgggaata accgatgacg
     2281 agtaattcaa caatgtgacc ggaataccca ggaaatccag ccccggcgca tccgcttttt
     2341 gcccgttctc gaaggcagtc agaattaatg gatgcactaa cgctccacca atcaccatgg
     2401 cagtaaatgg attaccgccg aagcgtttgc ccgcggtgta tcccaggatt atcgggaaga
     2461 acccaaacaa ggcatcactg gcgctgaata aaattaaata agtaccactt tgttcggtcg
     2521 tccactgaaa agtgagcgcc agagccagca tacctttcag gatcccggtt gccgccatca
     2581 gaccgatcag aggcgtaaaa atacctgaaa taacataaac aaagcggttt agcagattac
     2641 ctttctcatc attttccggt gcctgttgcg ctttttcgcc aaggcctgcc acgctgttaa
     2701 ccgccaggaa gacatcggcc acatggttac ctatgaccac ctgaaactga ccaccgcttt
     2761 ccaccaccat aataataccg ggggtctttt tcagtacctc agcttgcgct ttgctttcat
     2821 cctttaactt aaaacgtaat cgcgttgcgc aatgcatcag actcacaatg ttatctgcgc
     2881 ccccgactcc tgcgactatt tttctggcta actccgtcat aacttgccct ctaccgcttt
     2941 gcggcaaaac tccaaaaaaa aacctgaaaa aaacggcctg acgtgaatca agcaattttt
     3001 ttcaggtttt gcccgcttag tgcggtaaca atcctttact cagtaataat atttcagtgt
     3061 tctttgcgca cgcgctctat atttatggct aaaaacataa tctctgcggg tgaaatttta
     3121 cgttgatact gcaaaccaat aaaaatggcg atccgttccg cacattgcca tgcttgcggg
     3181 taattttgtt ttactgcttg ttgtaatgat tcatcactat cgttaattga agcatgttca
     3241 agaatacgcc aggataaaaa cttcagatgt gtaaccagtc gctgataact caagctttct
     3301 tcctggtaat taaggctgaa ctgaaatttt attaattgca gcatttcgcg cattaactgc
     3361 gtgacacctg caacatcctc catatttccg ctcatttggg cactgaccag atgcatggca
     3421 ataaagccca cttcatcttt cggtaactgc acgcccaacc gtttatcaat aatggttaac
     3481 gcttcttccc ctagctggaa ctctttcggg taaagccgct ggatatccca cagcaacacg
     3541 ttttgctgaa agcgtttaat cgcaaactgg caatggtcag ttagcgagat ataaatactg
     3601 tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa gacgatcaca ggttgccatc
     3661 acctcaagag gaatatgact taagagttcg cttaatcgcc cgttcagttc atgactgctc
     3721 aaggcatact ctttttctat tccacttgag ttaattcttt cgccagcgcg tttttgaaag
     3781 ccaattccgc gccccatgac gactttttcc cgctgttgat catcaataac caccacaaca
     3841 ttattgttga gaattttggt gatttgcatg ttcatagcaa ggaccttttt tataaacaaa
     3901 aaaaaaccga cttcaccagt attctctggt tatgtcaggt tttgcctgcg aatgcagtaa
     3961 caatccagtc atttattaat ggtttttata acgaacatcc aggttcggaa attaatttaa
     4021 ttgcgtgcag agaaaatagc aatgcgctat tgataaaaat atgaccatgc tcgcagttat
     4081 taactttgtg taattttagg aatttataaa gttatatata acaaatccca ataattaagt
     4141 tattgggatt tgtctggtga attatttgtc gctatctttt cccgccagca gtttatccag
     4201 ctcatcaccg cctacatgac ggaaatcctg acccttcacg tagtagaaga taaactcaca
     4261 aatattctgg cagcggtcgc cgatacgttc gattgaacgc gcacagaaca gcgcggtaag
     4321 tacgctcgga atagtgcgcg aatcttccat catgtaggtc atcagttgac gaacaatacc
     4381 ttcgtattcc tgatcgactt ttttatcttc acgataaata cgtaccgctt cgtcaatgtc
     4441 catccgcgcg aacgcgtcca gcacgtcgtg cagcatctgg atggtatgac ggcccagcga
     4501 ctccagactt accagcaacg gctgatgctg ctgggagaat ttctccagcg cagtacggca
     4561 gattttgtcc gccacgtcgc caatacgctc cagctcggca atggttttac tgatcaccat
     4621 aaccagtcgc aggtcgctcg ccgtcggctg acgtttggcg ataatgcgca cgcaggcttc
     4681 atcgatcgcc acttccatca tgttgacgtt cttgtcgcct tcgatcacgc gcttcgccag
     4741 atcgctgtcc tggttatgca tcgcggtgat tgcatcagaa agctgctgct ccaccatgcc
     4801 gcccatggtc atcacctgcg tgcggatact ttccagttcg gcgttgaact ggccggaaat
     4861 atgtttatta agattgagac tgtccataac gcactcctga atcaaccgta acgaccggtg
     4921 atgtagtctt ctgtttgttt cttcgctggc ttggtgaaca gatcgtccgt gttgctgaac
     4981 tcaatcaatt cgcccaggta cataaatgcc gtgtggtcgg aacaacgcgc agcctgctgc
     5041 atgttgtggg tgacgatcac cacggtgtaa tcctgcttca gttcggtgat cagctcttca
     5101 atacgcccgg tagagatggg gtcgagcgcc gaacacggtt cgtcgagcag cagcacttcc
     5161 gggcgaatgg cgataccacg cgcaatacac agacgctgtt gctgaccacc agagagagag
     5221 taaccgctct ggtgcaattt atctttggtt tcgttccaca atgcggcttt ggtcaatgcc
     5281 cactgcacgc gctcgtccat gtcggcacgg gagagcttct caaacagacg aacgccaaag
     5341 gcgatgttgt cgtagatgga catcgggaac ggcgtcggtt tctggaacac catgcccact
     5401 ttcgcacgca gcagggcgat atcctgagag ttggtcagga tgttgtcgcc atcaagcaga
     5461 atttcacctt ccgcacgctg ctccgggtac agttcaaaca ttttgttgaa ggtacgcagc
     5521 agcgtcgatt taccgcagcc ggacggcccg ataaacgccg taacctggtt tttggcgata
     5581 tccaggttga tgtttttcag ggcatggaat ttgccgtagt agaagttcaa attacgaacc
     5641 tgaattttac tcggggcagt ttcaaccata ctcattgcaa tcttttcctc atcgtacctg
     5701 atgcttttcg ctaatcaggc ttaccgtagg gccgggtaag gcgttcacgc cgcatccgga
     5761 aaagtgttaa ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag
     5821 gacgcacagg gtaatgatca ataccccggc ccaggccagt tgttgccatt ccgcaaacgg
     5881 gctcatcgca aacttaaaga tcgtcaccgg caggttggcg atcggctgca tcatgtccgt
     5941 gctccagaac tggttggaga gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg
     6001 ggcaatcgcc agcaggatac cggtcataat cccggacacc gacgctttca gcgtaatcgc
     6061 ggagatcatc ttccacttcg gtgtacccag cgcataagcc gcttcacgca ggctgtccgg
     6121 caccagtttc agcatgttct cagtggtgcg gataacgatc ggcacctgca acagcgccag
     6181 cgcaattacg cccgcccagc cggagaagtg ctccatctgt gccaccacga tggtgtagac
     6241 aaacagacca accacaatcg acggcgcgga gagcagaatg tcgttaatga agcgaatcac
     6301 ttctgccagc caggatttac gaccatattc cgccagataa atccccgcca taatgcccag
     6361 cggcgtaccg aataccgtgg cccacaaaat taacagcccg ctacccgcca gggcgttcgc
     6421 cagaccacca ccttccgtat tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat
     6481 accgtcgata ccgcgagtga tggtggacat taaaatccag atcagccaga acaggccgaa
     6541 ggccatcgtc gccatcgaga gcgtcagcgc aatacggttt ttgaggcggc gacgcgcctg
     6601 cattttgcgg cgagattcag ccagcgccgc agtggtttgc atttcaacca tagccattag
     6661 cgtgccccct cattcttagc caggcgcata atcataaact tcgatgcggc gaggacgatg
     6721 aaggtaatca caaacaggat caggcccagt tccatcagtg cggcaacgtg cagaccggat
     6781 tccgcttccg caaattcgtt cgccagcgca gaggtgatac tattgcccgg catgtacagc
     6841 gaggcgctgt cgagctggta ggtgttaccg atgataaagg tcaccgccat ggtttcaccg
     6901 agcgcgcggc ccagccccag catgatgccg ccgataacac catttttggt gaacggaaga
     6961 acgatacgcc agataacttc ccaggtggtg cagccaatac cgtaggccga ctctttcatc
     7021 atcaccgggg tttgttcgaa cacatcacgc attaccgccg caatgtacgg aataatcatg
     7081 atggcgagga tcacgcctgc cgcgaggata ccgataccaa atgcggggcc agagaacagc
     7141 gcgccaacaa tcgggatatt cgacatgata ttgccgaccg gctcctgaaa gtaaacggcg
     7201 aacagcggcg caaagataaa caggccccac atgccgtaaa cgatacttgg aatggctgcc
     7261 agcagctcaa tggcgatacc cagcgggcgt ttcagccagc caggcgcaag ctcagtcagg
     7321 aacagggcga taccgaaact caccgggacg gcgatcagca gcgcgataaa cgaagtcacc
     7381 aacgtaccgt agatcggcac cagcgccccg tagatatcgt tcggtgcatc ccactctttg
     7441 gtccatagga aagccagacc aaatttctga atgctcggcc aggaggagat gatcagagag
     7501 acaataatgc cacccaacat caatagcaca atcagcgccg ccagttttac cagcacgctg
     7561 aaaattatgt cgcccttttt acccggtggg ttaaaagcgg gcttggttgc agccataagt
     7621 tactcttcag ttaaacgcgt ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca
     7681 ccgtacccgg cctggagttt tattagtaca gcggcttacc gctactgtct ttaatattgg
     7741 tcttccacgc agcgcgaacc tgttcaacta cactatccgg caggctggcg taatccaggt
     7801 cgttcgcctg tttagccccg gttttgtacg cccagtcgaa gaatttcagc acctctgtgc
     7861 cttgttctgg tttcttctga tctttgtgga tcagaatgaa cgtggtagag gtaataggcc
     7921 atgcatcttc gcctttctgg ttggtcagat cctgagcgaa ggttttgctc cagtctgcac
     7981 cttttgctgc attagcgaag ttttcttcgg tcggactaac cggtttacca tcagcggaga
     8041 tcagtttggt gtacgccagg ttgttctgct tcgcgtaagc atattcaaca taaccaattg
     8101 cacccggcag acgctgaacg aacgcggcga taccgtcgtt acctttaccg cccagaccga
     8161 tcggccattt tacggtagag ccagtaccaa cgttgttttt ccactcttcg ttcactttcg
     8221 ccaggtagct ggtgaagacg aaggaagtcc cggagccatc tgcgcggcgt actacagcaa
     8281 tgttttgtga aggcagtttc agacccggat tcagtttggc gatggcttca tcatcccact
     8341 tcttgatttt gcccaggtag atgtcgccga gggttttacc atccagcacc agttcgccag
     8401 acttcagccc tggaatgtta accgccagca ccacgccgcc aatcacggtc gggaactgga
     8461 acagaccttc ctgagccagt ttttcgtcag acagcggcgc gtcagaggca ccaaaatcaa
     8521 cggtattagc gataatctgt tttacgccac cggaagaacc gataccctgg tagttaactt
     8581 tattaccggt ttctttctgg taagtgtcag cccatttggc atacaccggc gcagggaagg
     8641 ttgcacctgc acctgtcagg cttgcttctg caaacacaga gaaagcactc atcgataagg
     8701 tcgcggcgac aacagttgcg acggtggtac gcataacttt cataatgtct cctgggagga
     8761 ttcataaagc attgtttgtt ggctacgaga agcaaaatag gacaaacagg tgacagttat
     8821 atataaggaa tatgacattt ttatgacaga aaggaattac caaataactt actgatttta
     8881 aatgtgaaga tgaaaattcc acatgccatc tttttgtcat gatgcattta aaagtgataa
     8941 agaaatgggt gagatgacaa cgatgtgatg aacaatcgtt gtcagaccta ttagcgcaca
     9001 atctcaacag ttatcgtcgc tgttgcagta aattgccccg gggctgggcg agctccagta
     9061 aggctcgcag gagcagctga aaaagttaca ctacctgatt tttcaccata cactgttgat
     9121 ttatccatgt ctgttggcaa ctcaccaccg ttcactttga tttccttatt atttttgtcg
     9181 taaattttta tccccacgtt agagtttgaa gttacgacca ttgaattgtc cgtattgggt
     9241 tcggtagcat aaaaagacgt actgatatca tcggtattca gtatccccag gttttcacac
     9301 tcaatagtga ctgttttagt aattttatgt gttgttaccg cacttccaac aacagatgaa
     9361 aaatctgcag ctggtatttc tttaaaatca aaacaaactg gctttaaatt atttatttcg
     9421 caactttgcg gagctgtaat atcgccccga atataaacat cagccatcgg ctgtgagtaa
     9481 tccaccgggg tgtcacggct aattgttcca tacaatgtca ctatctttgt ttcaggaata
     9541 atcaattttc ctgcaaatgt cttcttgata taaaaatata ctatcgcttt agccccactg
     9601 gatatactta aaggttcaat gcggttgcaa ttgtactttg tattaccaga cgggttgtta
     9661 gcatgatatt caaaaggtgc attcacatac ccaaccccaa gaatatctat agcaatccca
     9721 acatcaaggt tcggattaag atcataataa ttaatattag agtgaacccc agcataagtt
     9781 aatgcctggt ttatttttga cgtataatag atatagggga attctcctac gttaccagta
     9841 tcacaatgac agcgtccaag atatgaggat ccattagtta cagtggtgtt aaatgtcgtt
     9901 ccaaccttat tttcgttcgc ggtaataa
//
LOCUS       DAJVQR010000127         9670 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVQR010000127 DAJVQR010000000
VERSION     DAJVQR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(25..213)
                     /locus_tag="OQM52_003533"
     CDS             complement(25..213)
                     /locus_tag="OQM52_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1565237.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(432..1409)
                     /gene="epmA"
                     /locus_tag="OQM52_003534"
     CDS             complement(432..1409)
                     /gene="epmA"
                     /locus_tag="OQM52_003534"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1565238.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1734..3542
                     /gene="frdA"
                     /locus_tag="OQM52_003535"
     CDS             1734..3542
                     /gene="frdA"
                     /locus_tag="OQM52_003535"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1565239.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3535..4269
                     /gene="frdB"
                     /locus_tag="OQM52_003536"
     CDS             3535..4269
                     /gene="frdB"
                     /locus_tag="OQM52_003536"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1565240.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4280..4675
                     /gene="frdC"
                     /locus_tag="OQM52_003537"
     CDS             4280..4675
                     /gene="frdC"
                     /locus_tag="OQM52_003537"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1565241.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4686..5045
                     /gene="frdD"
                     /locus_tag="OQM52_003538"
     CDS             4686..5045
                     /gene="frdD"
                     /locus_tag="OQM52_003538"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1565242.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5108..6241
                     /locus_tag="OQM52_003539"
     CDS             5108..6241
                     /locus_tag="OQM52_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1565243.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="OQM52_003540"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="OQM52_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1565244.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="OQM52_003541"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="OQM52_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1565245.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="OQM52_003542"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="OQM52_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1565246.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="OQM52_003543"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="OQM52_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1565247.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="OQM52_003544"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="OQM52_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1565248.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..9423
                     /gene="epmB"
                     /locus_tag="OQM52_003545"
     CDS             8395..9423
                     /gene="epmB"
                     /locus_tag="OQM52_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1565249.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2462 a         2478 c         2465 g         2265 t
ORIGIN      
        1 gcattatctg aacataaaac actatcagta agttggagtc attaccatta aggcgaatgg
       61 aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac ccattaagta
      121 ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca atatgagtcc
      181 tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt cagcgcgtgt
      241 ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta aaaaccaata
      301 cagaattgat acttggatga tatcaaatat ccagtcttaa attgtggcct ttaacggaaa
      361 aatgaaaaca tatgctgcag aatggcgctt atcacgccat tcttcgctgt taattcagta
      421 atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag tgtctccgcg
      481 cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca gtcaggcata
      541 ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa accgcgcgcc
      601 gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc cgttaattca
      661 tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc agcgacccga
      721 tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg gtacacaaag
      781 gtcggttttt ctttaccaat atttggctct acgccaaagg taaacagcaa ttgtagcaac
      841 gtgtcgcggt cttcttcggt atcagcaaca ttgctcaaat ccagtttcgc tgcgacttcc
      901 cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa gaaagcttgt
      961 tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag atcgtccacc
     1021 tcgttcatca accggtacat atcatagtgc ggtcgatacc actccagcat agtgaactca
     1081 gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg gaataccggc
     1141 ccacaaccag caaccagcag gcgtttcata tggtattccg ggctggtcat taaccagaga
     1201 ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac caaatgaata
     1261 tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc acgatcggca
     1321 aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg aatggatgcg
     1381 ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga caagggcacg
     1441 aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac agcggtgggc
     1501 agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc ttcagtgata
     1561 atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact tatccatcag
     1621 actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg taactttcag
     1681 gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa
     1741 cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt gctgcaattg
     1801 ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc
     1861 gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat catgacagct
     1921 tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag caggatgtcg
     1981 tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg tggggatgcc
     2041 catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg
     2101 agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg ctgttccaga
     2161 cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg gatattctgg
     2221 ttgatgatgg tcatgttcgc ggcctggtag caataaacat gatggaaggc acgttggtgc
     2281 agatccgtgc taacgcggtc gttatggcta ccggcggtgc gggtcgcgtt tatcgttaca
     2341 acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc cacggcgttc
     2401 cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt tccggtatcc
     2461 tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt
     2521 atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa aacaaatata
     2581 tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg cgtaaaggca
     2641 acaccatctc cacgccgcgt ggtgatgtgg tttatctcga cctgcgtcac ctcggcgaga
     2701 aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg
     2761 atccggttaa agaaccgatt ccggtacgtc cgactgcaca ctacaccatg ggcggtatcg
     2821 aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt gaatgttcct
     2881 ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa ctggtggtct
     2941 tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt aatggcaacg
     3001 aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat ctagttaacc
     3061 aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca atggaagaag
     3121 gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag ctggcagagc
     3181 tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc
     3241 tgctctacac cattgaactg agccacggtc tgaacgttgc tgaatgtatg gcgcactccg
     3301 caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc
     3361 gtgacgacgt caactttctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc
     3421 gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg
     3481 gcgaagcgga tgcagccgat aaggcggaag cagccaataa taaggagaag gcgaatggct
     3541 gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg
     3601 catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc
     3661 tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt
     3721 tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg
     3781 cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat
     3841 ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc
     3901 ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag
     3961 tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt
     4021 ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa
     4081 gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggtgta
     4141 tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct
     4201 gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa
     4261 ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc
     4321 cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt
     4381 tccggctgtg tggttcagca ttgaactgat tttcgcgctg tttgccctga aaaatggccc
     4441 ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct
     4501 gatcactctg acggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc
     4561 ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg
     4621 ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc
     4681 ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg
     4741 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
     4801 tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg
     4861 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
     4921 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtgcct gcgggcaaat
     4981 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt gtcgttacaa
     5041 tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
     5101 agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt actccacatt
     5161 tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca
     5221 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
     5281 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
     5341 aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga
     5401 aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttaccgcta aacagtggaa
     5461 tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc
     5521 ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg
     5581 ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
     5641 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct tccagccact
     5701 caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg
     5761 atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg aagcttatgg
     5821 tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttac ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
     9421 taactgatta aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc
     9481 ctacatcgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
     9541 acgccgcatc cggcaagagc aacaaacgca agtacagcaa ccactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata
//
LOCUS       DAJVQR010000128         9660 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVQR010000128 DAJVQR010000000
VERSION     DAJVQR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            279..1910
                     /gene="oppA"
                     /locus_tag="OQM52_003546"
     CDS             279..1910
                     /gene="oppA"
                     /locus_tag="OQM52_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1565250.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1996..2916
                     /gene="oppB"
                     /locus_tag="OQM52_003547"
     CDS             1996..2916
                     /gene="oppB"
                     /locus_tag="OQM52_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1565251.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2931..3839
                     /gene="oppC"
                     /locus_tag="OQM52_003548"
     CDS             2931..3839
                     /gene="oppC"
                     /locus_tag="OQM52_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1565252.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3851..4864
                     /gene="oppD"
                     /locus_tag="OQM52_003549"
     CDS             3851..4864
                     /gene="oppD"
                     /locus_tag="OQM52_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1565253.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4861..5865
                     /gene="oppF"
                     /locus_tag="OQM52_003550"
     CDS             4861..5865
                     /gene="oppF"
                     /locus_tag="OQM52_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1565254.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5918..6247)
                     /locus_tag="OQM52_003551"
     CDS             complement(5918..6247)
                     /locus_tag="OQM52_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1565255.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6282..7742)
                     /gene="cls"
                     /locus_tag="OQM52_003552"
     CDS             complement(6282..7742)
                     /gene="cls"
                     /locus_tag="OQM52_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1565256.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7885..8058
                     /locus_tag="OQM52_003553"
     CDS             7885..8058
                     /locus_tag="OQM52_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1565257.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8113..9366)
                     /gene="kch"
                     /locus_tag="OQM52_003554"
     CDS             complement(8113..9366)
                     /gene="kch"
                     /locus_tag="OQM52_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1565258.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2524 a         2272 c         2406 g         2458 t
ORIGIN      
        1 actttccggg caaccgccag acgattcttt attggtaatg agaataatta acaattaaag
       61 agcgtcgcga aagaataatg tgtctcgaca ggggagacac agtacgaatc gacataaggt
      121 gatcgtctga atcaccagaa taaataaagt cggtgatagt aatacgtaac gataaagtaa
      181 cctgacagca gaaagtctcc gagcttgtgc agggtcccaa tccgggatta cacatgctgg
      241 ttaataccag taattataat gagggagtcc aaaaaacaat gaccaacatc accaagagaa
      301 gtttagtagc agctggcgtt ctggctgcgc taatggcagg gaatgtcgcg ctggcagctg
      361 atgtacccgc aggcgtcaca ctggcggaaa aacaaacact ggtacgtaac aatggttcag
      421 aagttcagtc attagatccg cacaaaattg aaggtgttcc ggagtctaat atcagccgag
      481 acctgtttga aggcttactg gtcagcgatc ttgacggtca tccagcacct ggcgtcgctg
      541 aatcctggga taataaagac gcgaaagtct ggaccttcca tttgcgtaaa gatgcgaaat
      601 ggtctgatgg cacgccagtc acagcacaag actttgtgta tagctggcaa cgttctgttg
      661 atccgaacac tgcttctccg tatgccagtt atctgcaata tgggcatatc gccggtattg
      721 atgaaattct tgaagggaaa aaaccgatta ccgatctcgg cgtgaaagct attgatgatc
      781 acacattaga agtcacctta agtgaacccg ttccgtactt ctataaatta cttgttcacc
      841 catcaacttc accggtgcca aaagccgcta tcgagaaatt cggcgaaaaa tggacccagc
      901 ctggtaatat cgtcaccaac ggtgcctata ccttaaaaga ttgggtcgta aacgaacgaa
      961 tcgttcttga acgcagcccg acctactgga acaacgcgaa aaccgttatt aaccaggtaa
     1021 cctatttgcc tattgcttct gaagttaccg atgtcaaccg ctaccgtagt ggtgaaatcg
     1081 acatgacgta taacaacatg ccgatcgaat tgttccagaa gctgaaaaaa gagatcccgg
     1141 acgaagttca cgttgatcca tacctgtgca cttactatta cgaaattaac aaccagaaac
     1201 cgccattcaa cgatgtgcgt gtgcgtaccg cactgaaact aggtatggac cgcgatatca
     1261 ttgttaataa agtgaaagcg cagggcgaca tgcccgccta tggttatact ccaccgtata
     1321 ctgatggcgc aaaattgact cagccagaat ggtttggctg gagccaggaa aaacgtaacg
     1381 aagaagcgaa aaaactgctg gctgaagcgg gttataccgc agacaaaccg ttgaccatca
     1441 acctgcggta taacacctcc gatctgcata aaaagctggc gattgctgcc tcttcattgt
     1501 ggaagaaaaa cattggtgta aacgtcaaac tggttaacca ggagtggaaa acgttcctcg
     1561 acacccgtca ccagggcact tttgatgtgg cccgtgcagg ctggtgtgca gactacaacg
     1621 aaccaacttc cttcctgaac accatgctgt cgaacagctc gatgaatacc gcgcattata
     1681 agagcccggc ctttgacagc attatggcgg aaacgctgaa agtgactgac gaggcgcagc
     1741 gcacagctct gtacactaaa gcagaacaac agctggataa ggattcggcc attgttcctg
     1801 tttattacta cgtgaatgcg cgtctggtga aaccgtgggt tggtggctat accggcaaag
     1861 atccgctgga taatacctat acccggaata tgtacattgt gaagcactaa tggcaatacg
     1921 tggggcagga gtgtcctgct ccacggtgtc tgatttttat cgcattacag aaggcacagg
     1981 ccagaaggta gggcaatgtt aaaatttatt ctacgtcgtt gtctggaagc gattccgacg
     2041 ctatttattc ttattactat ttcgttcttt atgatgcgcc tcgcaccggg aagccctttt
     2101 accggcgaac gtactttacc gccagaagtg atggccaata tcgaagcgaa atatcatctt
     2161 aatgatccga tcatgacaca gtatttcagc tacctgaaac aactggcgca tggcgatttc
     2221 ggtccatcgt ttaaatataa agattattcg gtcaatgacc tggtggcatc cagttttccc
     2281 gtttctgcca aactgggagc cgccgcattt ttccttgcgg taatactggg tgttagtgct
     2341 ggcgttattg ccgcattaaa acaaaacacc aaatgggact ataccgtgat ggggctggca
     2401 atgaccgggg ttgttatccc cagttttgtg gttgcgccat tattagtcat gatatttgcg
     2461 atcattttgc attggctgcc gggcggtggc tggaatggtg gggcgcttaa attcatgata
     2521 ttgccgatgg tggcgttgtc actcgcctat atcgccagta ttgcgcgtat tacccgtggc
     2581 tctatgattg aagtattaca ctccaacttt attcgtactg cccgggcgaa agggttacct
     2641 atgcggcgga tcattttacg ccatgcatta aaacctgctc tgttacccgt gctctcctat
     2701 atgggccccg catttgtcgg cattattacc ggttctatgg tcatcgaaac catttatggt
     2761 ttgccgggga ttgggcaact gttcgttaat ggcgcattga accgtgacta ttccttagtg
     2821 ttaagcctga ccatcctggt tggtgcgtta accattttgt ttaatgccat tgtcgatgtg
     2881 ctatatgcgg ttatcgaccc gaaaatccgt tactgatact ggagctcgcg atgatgttaa
     2941 gtaagaaaaa cagcgagacg ctggaaaatt tcagtgaaaa gctggaggtc gaagggcgca
     3001 gcttgtggca ggacgcacgt cgacgtttta tgcataaccg tgcggcggtt gccagtctga
     3061 tagtgctggt gctgatcgcg ttatttgtaa tcctggcacc gatgctttcc cagtttgcct
     3121 atgacgatac tgactgggcg atgatgtcca gcgccccgga tatggagtcc ggtcactact
     3181 ttggtactga ctcatccggt cgcgacctgc ttgtgcgcgt tgcgattggc gggcgtatct
     3241 cactcatggt cggtgttgct gcggcactgg tggcagtggt cgtggggaca ctttatggtt
     3301 cgctttccgg ttatctgggc ggtaaagtgg attcggtaat gatgcgtctg ctggaaatcc
     3361 tcaactcctt cccattcatg ttcttcgtca ttttgctggt gacctttttc ggccaaaaca
     3421 tcctgctgat tttcgtggcg attggcatgg tttcctggct ggatatggct cgtattgtgc
     3481 gtgggcaaac cctgagtctg aagcgcaaag agtttattga ggcggcacaa gttggcggtg
     3541 tatcgacgcc gggcattgtt attcgccaca ttgtgccgaa cgtactcggt gtggtggtgg
     3601 tctacgcatc gctactggtg cccagcatga tcctctttga atctttcctt agcttcctgg
     3661 ggttgggtac gcaagagccg ttaagcagct ggggggcatt gctgagtgat ggcgcgaact
     3721 cgatggaagt ctctccatgg ttactgttgt tcccagcggg attccttgtg gtgacactgt
     3781 tttgtttcaa ctttatcggc gatggcttgc gtgatgccct cgacccgaaa gatcgttaag
     3841 gagtgcagcc atgagcgtaa ttgaaactgc aactgtgccg ctcgcacaac aacaggctga
     3901 cgcactgctg aacgtgaaag atttgcgtgt cacctttagt accccggacg gcgacgtcac
     3961 ggcggtcaat gatttaaatt tttccctacg tgccggagaa acgctgggca ttgtaggtga
     4021 gtctggttcg ggtaaatcgc aaactgcatt tgcgttgatg ggcctgttgg ctgccaacgg
     4081 acgcattggc ggatcggcaa ccttcaatgg gcgtgaaatc ctcaatttgc cagagcgtga
     4141 actcaataaa ctgcgcgctg aacaaatctc aatgattttt caggacccaa tgacttcgtt
     4201 gaatccctat atgcgcgtcg gtgagcagtt gatggaagtg ctgatgctgc ataagaacat
     4261 gagcaaagct gaggcgtttg aagagtcggt gcggatgctc gatgcggtaa aaatgccgga
     4321 agcacgtaaa cgcatgaaaa tgtacccgca cgaattttct ggcggcatgc gtcagcgagt
     4381 catgattgcg atggcattgc tatgtcgacc taagttgctg attgcggatg aacccactac
     4441 agcgctggac gtcaccgtac aggcgcagat catgacgcta ttgaatgaac tgaagcggga
     4501 atttaatact gccatcatta tgatcaccca cgatcttggc gtggtggcgg ggatctgtga
     4561 caaagtgctg gtaatgtacg ccgggcgcac gatggaatat ggcaatgcgc gcgatgtctt
     4621 ttatcaaccc gttcatcctt attctatcgg tttgctcaac gcggtgccgc gtcttgatgc
     4681 ggaaggtgaa acaatgttga ccatcccggg taatccgcca aacttgctgc gattaccgaa
     4741 aggttgcccg ttccagccac gttgtccaca tgcgatggaa atttgtagta gcgcaccgcc
     4801 gctggaagag tttacgcctg gccgtctgcg tgcttgcttt aaaccggtgg aggaactgtt
     4861 atgaatgctg taactgaagg aagaaaagtc ctccttgaaa tcgccgatct taaagtgcac
     4921 tttgaaatca aagatggcaa acagtggttc tggcaaccgc cgaaaacgct caaagccgtc
     4981 gatggtgtca ctcttcgcct gtatgaaggg gaaacattag gtgtggtagg ggaatcggga
     5041 tgcggtaagt ccacctttgc tcgcgccatc atcggtttgg tcaaggcgac cgacggtcat
     5101 gttgcctggt taggtaaaga gttgctgggc atgaagcccg atgaatggcg tgccgttcgc
     5161 agtgatattc agatgatttt ccaggatccg ttggcatcgc taaacccgcg tatgaccatc
     5221 ggcgagatca tcgctgaacc actgcgtact tatcatccga aaatgtcacg ccaggaagtt
     5281 cgcgagcgcg tgaaggcgat gatgctgaaa gtcgggttat tgcctaacct gattaaccgc
     5341 tatccgcatg agttctccgg tgggcagtgc cagcgtatcg ggattgctcg tgctcttatt
     5401 cttgaaccga agctgattat ctgcgatgag ccggtttcgg cgctggacgt gtcaattcag
     5461 gcgcaggtgg ttaacctgct ccagcagctg caacgtgaga tgggattgtc attaattttt
     5521 attgctcatg acctggccgt ggtaaaacac atttccgatc gtgtgttggt gatgtatctc
     5581 ggccatgcag tagaactggg gacctatgat gaggtctacc acaatccact acatccttac
     5641 accagggcat tgatgtcggc agtccccata cctgatccgg atctggagaa gaacaaaacc
     5701 atccagttac tggaagggga attaccgtcg ccgatcaacc cgccttccgg ttgtgttttc
     5761 cgtacccgtt gcccgattgc cggtccggag tgcgccaaaa cacgtcctgt tctggagggg
     5821 agtttcagac acgccgtttc ttgcctgaaa gtcgatccgc tttaaaaaca ataagggctg
     5881 acagttgtca gccctttttc acgctaaaag cgattattta ttcccgccag atgatatggc
     5941 aaagtttgtg atctttttcg cggcataaca gaatgcgggc gaaaacatcg ctgatttcac
     6001 catcttcact gtccgccaga ccaatcacca cttcggcaaa aaagtccggg ttcaaatcga
     6061 aatcaacatg ttcctgccag tcttccgccg gatcgaataa ctccgcgccg ccgcgctctt
     6121 caaactgaag attgaacagc agaacatcag ctggatcgag attgtcggca gccagttcga
     6181 gaaaaatatc gtaggcctgc tcaagcgttt catcttcagt caggcgattg tttagatcca
     6241 tatccatagt cactacctgt ttaacctctg ttggcgacgt tttacagcaa cgggctgaag
     6301 aagtaaaaca gtcgctcggc gacacgttgc cataatggac gttttagcca taaacgggca
     6361 tcgagcagac gtgaacgcga aatataatcg tcctgaacgg cggcaaggtc tgcaccaaaa
     6421 cctttatcgt cgattgccaa ggtaatctcg aaattcagcc acagactacg catatcaagg
     6481 ttaacggtgc caaccagact tagttcgcca tcgaccagca cgctcttggt atgcagtaac
     6541 ccgccttcaa actgataaat tttaacccca gcagcaagca gttccgtaaa gaatgcgcga
     6601 ctggcccagc cgaccagcat cgagtcattt tttcgcggaa ggataatact gacatccacc
     6661 ccgcgctgcg ccgccgtgca aatcgcatga agtaaatcat cgcttggcac aaagtagggc
     6721 gtggtcatga tcaaatattc atgcgccgaa taagccgcag tcaataatgc ctggtgaatg
     6781 agatcttccg gaaagccggg gccagaagca attgtgtgaa tggtgtgacc gctggcctgt
     6841 tcaaacggca taatattgac atctggtggt ggcggcagaa tacgttttcc ggtttcaatc
     6901 tcccagtcgc aggaataaat aatccccatc gcggtggcga tggggccttc catacgcgcc
     6961 atcagatcaa tccattgccc tacgcccgca tcttgtttga agtagcgagg atcgaccata
     7021 ttcatgctgc cggtgtacgc gatgtaatta tcgatcatga tcatcttgcg atgttggcgc
     7081 aggtccatac ggcgtaaaaa cacacgcatc agattgacct ttaaggcttc gaccacttca
     7141 ataccggcat tacgcattag ctcgggccac gggctgcgga aaaaagccac actcccggcg
     7201 gagtcgagca tcaatcggca atgaatacca cgccgtgcag ccgccattaa tgattcagcc
     7261 acctggtccg ccatgccgcc gggctgccag atataaaaca ccatctcaat attatggcgc
     7321 gcgagctgga tgtcgcggat taacgcctgc atcacatcat ctgactcggt catcagctgt
     7381 agctgattcc ctttgacccc agcgatcccc tgacgacgct cgcaaagctt gaataatggc
     7441 gcagcgacac tgctattttc ttcggcgaag atatgcttac aggctttaag gtcgttaagc
     7501 cattttgcgg tggaaggcca catcgctctg gcgcgctcag cgcggcgttt gcctaaatgg
     7561 agctcgccaa cggcaagata ggcaataatt ccgactaacg gcagaatgta aataatcaac
     7621 agccaggcca tcgcggaggg aactgcgcgt cgtttcatta gaatgcgtaa agttacgcct
     7681 gcaatgagca accagtatcc cagaatggcc aaccaactca ccaacgtata aacggttgtc
     7741 atagattaga aatccttttg aaagcgcatt gttatgagtt tacgcatatg gattcatctg
     7801 gcaaataaaa acgcgggaaa agcactggtc agcgtcgggg gcgggggtat aatgaccatt
     7861 ctgttattgc atagagtagt taacatgaag cggagtagaa cggaagtggg gcgctggcgc
     7921 atgcagcgtc aggctagccg acgtaaatcg cgttggcttg aggggcaatc gcgccgaaat
     7981 atgcgtatcc acagcatcag gaagtgcatt ctaaacaaac agcgtaactc gttattgttt
     8041 gcgatctaca atatctaaat gtactgggca ccattgcggt gcccaataag tttactcttc
     8101 cgtctttaaa ctctattttt gcgccgattc tttactgtca gctttcgttt cttgttcatc
     8161 gttatcgctg aaaataccat gacctgagtt caacaacatt gaaacgagca tatcattatt
     8221 aatctcttca ccatttaaaa ctcgcgccag aatttcgctg ccaaacagtt gcggtgagag
     8281 aatgatatcc ggatgtacca tcttaatctt attcaggttt ttgctatcac tgacggcgag
     8341 aactgttttg acatcactgc tcatatcttt tgccgagagt acaacaaacg cgttatctgc
     8401 atcgttatca ctcagcgcca gaatggcccg gcaacgatcg attcccgctt tctttaatac
     8461 tgaactgtca ttactgtcac cggggataac atcagcgtta tcgcctaagc gttgctcaag
     8521 ttgcttgata tcatcttcag gcaagttgct gataaccgtt acgttttgtc cgcgttgatt
     8581 cagttgcagg atcgtattga tggcgagaat cgaatgtccg caaacaataa aatgatcttt
     8641 acgatgcatt gtatgattgt ttccttttaa aagtttgttg aatcccccgc ggataagcgg
     8701 accaaaaatt gaggtcatag atgtggcaaa aacggtaatg ccggaaataa tgaccgaaat
     8761 agtgaacaat cgtgctgatt cagaaacagg gacaatatct ccatagccga cggttgacat
     8821 ggtttctatc gaaaaataga acgcggtcat caaactttct attcgcggat taaaaccttc
     8881 gcttaaataa agcgcaccgt aggttgagta aaacagtaat gtcgtgaaac taataaatgc
     8941 aaaaattgtc ccggctgcgg cgctactgtg ggagaagtct ttgcgcagta tcagcaaaaa
     9001 caccagcgta aaaatgcaaa atccaatact aaatttcagc cagggataaa aatgcagggt
     9061 gtaaattagc gctatcaaca acaaaatgat actgattgcc catgcgagct ttgcccggaa
     9121 cagtaagcca atggagttca ggacaagaaa gacgccgagc ataaatagtg gggcgtttgc
     9181 cagcaaggag agatccaact ctgaaaatgc tttgatatgg aaaatatcaa gcaaattgac
     9241 cgacatacca tagattgttt taaaaataag caatccattt aaaaagacgg ccagcgcgag
     9301 aatgtcgtgc cgtagggtca cccataaatt tgttgctgtt tgtttgaatg tagcccagtg
     9361 actcactgat acctctgtaa ccacaatttt gtttaattat cttagcgtgc acattttctg
     9421 aacatacatg cagcgcgtga atgtgttaaa caatagtaga tgactttatc cgcagtaatc
     9481 aaaggttttg agtgagcaac tctgcgtcag gaaagggctt ttttcctgaa catgctcaca
     9541 tcatataaag aaatacagat aaagttatta tctgcttgtg gtagtgaatg cactgaccgg
     9601 ctataaggaa aggccaaaca agaacacggt tgcaaaaacc gtgcccttaa atattggatc
//
LOCUS       DAJVQR010000129         9601 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVQR010000129 DAJVQR010000000
VERSION     DAJVQR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(12..332)
                     /gene="psiF"
                     /locus_tag="OQM52_003555"
     CDS             complement(12..332)
                     /gene="psiF"
                     /locus_tag="OQM52_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1565259.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(451..1866)
                     /gene="phoA"
                     /locus_tag="OQM52_003556"
     CDS             complement(451..1866)
                     /gene="phoA"
                     /locus_tag="OQM52_003556"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1565260.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1967..2227)
                     /gene="iraP"
                     /locus_tag="OQM52_003557"
     CDS             complement(1967..2227)
                     /gene="iraP"
                     /locus_tag="OQM52_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1565261.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2409..2612)
                     /locus_tag="OQM52_003558"
     CDS             complement(2409..2612)
                     /locus_tag="OQM52_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565262.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2690..3784
                     /gene="ddlA"
                     /locus_tag="OQM52_003559"
     CDS             2690..3784
                     /gene="ddlA"
                     /locus_tag="OQM52_003559"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1565263.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3808..4020)
                     /locus_tag="OQM52_003560"
     CDS             complement(3808..4020)
                     /locus_tag="OQM52_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1565264.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4280..4588
                     /locus_tag="OQM52_003561"
     CDS             4280..4588
                     /locus_tag="OQM52_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1565265.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4647..5741)
                     /gene="yaiW"
                     /locus_tag="OQM52_003562"
     CDS             complement(4647..5741)
                     /gene="yaiW"
                     /locus_tag="OQM52_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1565266.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5754..6974)
                     /gene="sbmA"
                     /locus_tag="OQM52_003563"
     CDS             complement(5754..6974)
                     /gene="sbmA"
                     /locus_tag="OQM52_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1565267.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7326..8483
                     /gene="ampH"
                     /locus_tag="OQM52_003564"
     CDS             7326..8483
                     /gene="ampH"
                     /locus_tag="OQM52_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1565268.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8484..8987)
                     /locus_tag="OQM52_003565"
     CDS             complement(8484..8987)
                     /locus_tag="OQM52_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1565269.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9075..>9601)
                     /locus_tag="OQM52_003566"
     CDS             complement(9075..>9601)
                     /locus_tag="OQM52_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1565270.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2383 a         2450 c         2285 g         2483 t
ORIGIN      
        1 tcaatcaggt atcaggcggc tttcttgagg caggcactca taaacttatt acgatcatca
       61 cctttcagag cttgttgcgt tgcttgatta ttacattcac gcatcttttg ctgctgtggc
      121 gtcaaacttt tttcaccagg cgcagacttg ctgttcttca ggcaatcact catgtaggtc
      181 ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct gattacagga ggtcatacgc
      241 tgttgttgtg gggttaaggt tctctcggca gcgccgacgg tggttaaaaa aaccagacca
      301 aaaagcaagg taaccagtaa tgttattttc atagcaccat ccctcttcat gttttaacca
      361 tgagcgtttg cgcccgtgat ctgccattaa gtctggttgc taacagcaaa aaaaccaccc
      421 ggcagcgaaa attcactgcc gggcgcagtt ttatttcagc cccagagcgg ctttcatggt
      481 gtagaagaga tcggtctggt cggtcagtcc aacaacattg gcggcatgcg ggccatacgc
      541 cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc tcttcggaat tcccgtaact
      601 catcaccatc actgcgccat ctttagtatt tagcgcctga gtgaggcccg gcgctttggt
      661 atccggcgca acaacctggc tggcgtgggc gtgatcagcg gtgactatga ccagcgtgtt
      721 accctccttt ttagcgaatt ccagcgcccg ttgtacggct tcatcgagat cgaccgtctc
      781 gccaatttgc ccacaagggt tcgcagcgtg atcctgttta tcgattgacg caccttcaac
      841 ttgcaggaaa aatcctttct catttttact caacaattca atggctttgt cggtcatctg
      901 tgccagggtt ggtacactgt cattacgttg cggattaggc gtataggtga ctgcgggctt
      961 atcgatattg ccgtggtacg ttgctttcgg ccctagccag cgcactggca tattgccgtc
     1021 agcaaacagg ccaagcaggg gtttttgctg attcgcttcc gtcaccgaat tcagtgaggc
     1081 agcatcgttc accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg tttttccctg
     1141 ccattcacca gcggttgccg tttcagcaaa ggtttttgcg ccgccgccaa gcgtaacgtc
     1201 ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt ccagagcgtt
     1261 acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgaag tcacatgtgc
     1321 caccagcgca gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac cggtcgccag
     1381 acctgcggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa tatcgacgcc
     1441 cagcgcgccg ttataggttt tgacatcggt tgaccaggcg gttgctgatg cagccgagtc
     1501 ggtgacgtag tccggtttgc cagttttttt attcagcgca tagtgagtgt attgcccggt
     1561 aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat aattacgtgc
     1621 ggcagtaatt tccgaatccc ccatcccatc gccaatcagc aaaataatat tttttgcagg
     1681 tttatcgcta agagaatcac gcagagcggc ggtctgatca cccgttaagc ggcgagcacc
     1741 gccgggtgca gtaatatcgc cctgagcagc ccggttttcc agaacaggca tttctggtgt
     1801 ccgggctttt gtcacagggg taaacagtaa cggtaagagt gccagtgcaa tagtgctttg
     1861 tttcacttta ttttctccat gtacaaatac attaaaaaat aaaaacaaag cgactataag
     1921 tctcggccgt gacaacttta tgacagctgt tgaaaagatt aactttttac tgacaaggat
     1981 gcttcaataa cttctttacg taatcgcgca gcagctctgt atcgtcgtca ggaatgctgg
     2041 catcgggctt tacctcgtac agcgccccct ctacctgatc aatcaaccgc tgttggtcat
     2101 tttgcgccat attgcgaagc attgcagtga cgataatctc caaagcttct acctgcgcac
     2161 acagttcttt cgactcttct tctttttggg caagcttaaa taacaactca gcaatgagat
     2221 ttttcatgtc tgtatttcct tatccaaagt atggagaagt taacattatg tttcacaact
     2281 tcatagtgtc tggaagtatt atttttatga caaaggaaat actttgcgca aatatttttt
     2341 gtttgattgc cagcgaaacg tttctcttat ttaaataatt acccacacaa aatataggct
     2401 tttaattatc aacctttatt ataaataaaa gctatgaaat atattctctt cgttaactta
     2461 taattaagag aaaaaaactc accgcattgc catttaagcc aggaataaat tagcttcccg
     2521 ttcatcattt ttgtgatctt gcgcacatct ttgagataac gcactttctt caccagtacg
     2581 ttgcacgctt tgtcagcaga agattgcaac aatctttcag aaggccgcga atcccccgtt
     2641 ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga tggaaaaact
     2701 gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc
     2761 aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc tgggcattga
     2821 taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg cagacgatcc
     2881 tgcccatatt gcgttgcgcc cttcggcgac cagccttgcg caggtgccag gtaaacatga
     2941 gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg tcattttccc
     3001 gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa
     3061 tttaccgttt gtaggttctg atgttctggc ttcagcagcc tgtatggata aagatgtcac
     3121 caaacgtctg ctacgcgatg ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc
     3181 taatcgtcac aacatcagtt ttgccgaagt ggagtctaaa ctggggttac cactgtttgt
     3241 aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg aagaacagta
     3301 cgcaattgcc gtcgatctgg cgttcgagtt cgatcataaa gtgatcgttg agcaagggat
     3361 caaaggtcgt gagatcgaat gcgcagttct gggcaacgac aatccgcaag ccagcacctg
     3421 tggcgagatc gtactcacca gcgatttcta tgcctacgac accaagtaca ttgacgaaga
     3481 tggcgcgaaa gtggtagttc cggcagccat tgcaccagag atcaacgata agatccgggc
     3541 gattgccgtt caggcttatc agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt
     3601 tttaacccca gagaacgaag tggtgatcaa cgagatcaac acactgcctg gcttcaccaa
     3661 catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg atctgatcac
     3721 acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat
     3781 gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt catcgcgacg
     3841 acgaataatc aaccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata
     3901 ggtctgccaa ccttttgctt caaatcccag tacccccacc acgccattaa gaatgaatat
     3961 aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcagattcat
     4021 ggcagctccg ttaacgaaaa acaacatcct tgcatattct gtaacaaccg tctgtggtgt
     4081 agacaacatt ttcgtaatta ttaataaatt taacttaagg ttgccatttt gtgactcaca
     4141 acgaagattc acgcttcggg aatattccca acatcaattt accatgattc tgatattgac
     4201 aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag
     4261 atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa
     4321 atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg
     4381 cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg
     4441 tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac
     4501 cattttcggg ctgatcccgt ttttagcagg ctgcctcatt tttgcagtgg tggcgctatg
     4561 gctgcactgg cgacatcgcc gccagtaaaa tttacgttgc cagatgcggc ctgaaacgcc
     4621 ttatctggcc tgacattcct gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct
     4681 acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga
     4741 atgccaggca acatttcgcg gggtagtgat ttacccgttt tcgcttcggc aagttgataa
     4801 actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc taactgacgg
     4861 cgaatttcgc tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt
     4921 ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg
     4981 ctggccttac tgactgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta
     5041 aaatcagcaa agcggtatat cggtgcgcta tagctggcgg gataattcag taaatggtaa
     5101 gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt accgtccatt
     5161 ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg catcggccca
     5221 ccggtgcgca ccggattaag cgaaccaaac agcgtctgcc ccattggtac catgctgatc
     5281 agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa ccgttcgcta
     5341 taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa agcaggaatg
     5401 tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa gcccggaacg
     5461 gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga gtagacgttt
     5521 tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt cgcccaggca
     5581 tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac atctatcgct
     5641 ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa aaccagaacc
     5701 gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc tttttagctc
     5761 aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga
     5821 cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa
     5881 gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag cgtaatcgta
     5941 ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag
     6001 agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata gtttttccgt
     6061 acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca
     6121 taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc cagccccggc
     6181 agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac gatagcggca
     6241 atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg cgcggagagc
     6301 gttaccagca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa actgacgccc
     6361 atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct
     6421 tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag
     6481 cggaacacgt agtgactgac aaagaagttg ttcagtacac tgatcaccac cgcgatcagc
     6541 gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac tttatgcggc
     6601 gaacttagcg cggtttgaat cagatcatag aacggcgcat accaggcgtt gacggcgacc
     6661 ccgacttcca ccaaaaacca agtgacgaag ataatcagcg cagtaccgag tattgaccag
     6721 tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc tacgcaaaca
     6781 atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg
     6841 atctgcccgg aagcgccggt gatacgtgcc acccagtcac ccccaccggc ttgccagaag
     6901 ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa
     6961 aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt
     7021 aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga cccatagcaa
     7081 ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac aaaccaaaga
     7141 ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga
     7201 aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt
     7261 tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat ctatggacac
     7321 caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg
     7381 tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc gatcgttatg
     7441 ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta
     7501 accagcgcgt ctttcgtagt tatggtgaaa cgcgacctgg taataacgtt cgcccgcagc
     7561 tggattcggt cgtgcgtatt gcttccctca ccaaactaat gaccagtgaa atgctggtga
     7621 aattgctcga ccaggggacc gtaaagttaa acgatccgct aagtaaatat gctccgccag
     7681 gcgcacgcgt gccgacctac aatgggacgc ccattaccct agtgaatctg gcaacccata
     7741 ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc
     7801 caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca
     7861 gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg gcgaatgcgt
     7921 ccggcaagcc ttatacccag ttgtttgaag agcagattac ccatccattg gggatgaaag
     7981 acaccaccta caccccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg
     8041 ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg
     8101 gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag cgcagtaacc
     8161 aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa gtgattggca
     8221 tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag
     8281 agggtcgtcc gaggattatt cagaagacag gcggtggtgg cggtttcatt acctatatgg
     8341 cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc
     8401 gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc gggaataaac
     8461 cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt
     8521 ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttctgcgacg
     8581 acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc
     8641 aattcttcat tccagtcaat cattgataat aatgttgcgc gggtttgttt ggctggtaga
     8701 tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt tttcattaaa
     8761 ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac gttttcttct
     8821 ctacgcagag agataactcc ttctaaaata acgaaagtat cttcgttatt gacatcactg
     8881 gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct ggacaaacat
     8941 ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg cgcgtccttt
     9001 gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc agtatttctt
     9061 cggagtgaag aatattacca agtatattta acacccacgt tcgcggacca gtcttgatct
     9121 acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt ggtgaagctg
     9181 aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc gatggaatca
     9241 ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt gaagtacgga
     9301 gtcagagcct gatcttcgct gtaggtgaag gtataacctg catctacacc cagttcataa
     9361 cgcatgctgt cgtaaggctg accgtcaact ttcatgtcgt tgctcagctg gtagtcatca
     9421 ccagactgga acagaccaga aatgctgccg taaggagtca cgtaaccagc atcacccagt
     9481 ttgaagtcgt aaccggtttt caaaccgaag ccccaagcgt cggagttggt gctaccgtca
     9541 acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag
     9601 c
//
LOCUS       DAJVQR010000130         9586 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVQR010000130 DAJVQR010000000
VERSION     DAJVQR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..580)
                     /gene="pflA"
                     /locus_tag="OQM52_003567"
     CDS             complement(<1..580)
                     /gene="pflA"
                     /locus_tag="OQM52_003567"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1565271.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGN"
     gene            complement(772..3054)
                     /gene="pflB"
                     /locus_tag="OQM52_003568"
     CDS             complement(772..3054)
                     /gene="pflB"
                     /locus_tag="OQM52_003568"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1565272.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3109..3966)
                     /gene="focA"
                     /locus_tag="OQM52_003569"
     CDS             complement(3109..3966)
                     /gene="focA"
                     /locus_tag="OQM52_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1565273.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4372..6132)
                     /locus_tag="OQM52_003570"
     CDS             complement(4372..6132)
                     /locus_tag="OQM52_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS1565274.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6262..6888
                     /locus_tag="OQM52_003571"
                     /pseudo
     CDS             6262..6888
                     /locus_tag="OQM52_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7087..8175
                     /gene="serC"
                     /locus_tag="OQM52_003572"
     CDS             7087..8175
                     /gene="serC"
                     /locus_tag="OQM52_003572"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1565275.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8246..9529
                     /gene="aroA"
                     /locus_tag="OQM52_003573"
     CDS             8246..9529
                     /gene="aroA"
                     /locus_tag="OQM52_003573"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1565276.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2434 a         2301 c         2410 g         2441 t
ORIGIN      
        1 cgttacccat atcacgggta aattcaccga ggcgatgtgc cgaatcgtca tcgtcagacc
       61 agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc agatatttag
      121 cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc tcgtcgttca
      181 tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca atcaccggat
      241 cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg aatgccttct tttttacagg
      301 cgcggaacca gtcacgaaca aactcagctt gcaggattgc ttcaccgccg gatgcggtaa
      361 cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc atcaaatctt
      421 caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga caatacaggc
      481 agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acctgggccg tctacggttc
      541 cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt ctccagatgt
      601 ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct gttttggcag
      661 tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag gccccacttt
      721 cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta attacataga
      781 ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg agttgaaacg
      841 tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt tttccatcgc
      901 gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac cttcgatgga
      961 tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac gaacttcgtc
     1021 gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat ctttagcgta
     1081 agcaaacggc agtttagcaa cggaagtcag agacgctaca gcacctttct ggtcacgacc
     1141 gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt ctggggtgtt
     1201 accagttttc ttaccataca caacgttaga agtgatggtc agaacagact gagtcgggat
     1261 agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta ccaggtcaac
     1321 agccaggtca tctacacgcg gatcattgtt accaaactgc gggtattcgc cttcgatttc
     1381 gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat atttgattgc
     1441 agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg tgcggataac
     1501 gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt gcatgtagtg
     1561 gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt gatccatgcg
     1621 ctccatcact tcatcgtagc tcaggagatc gcctttgatc ggttcagact tcggaccaac
     1681 ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg ttttcgccag
     1741 gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc ttacgcagca
     1801 agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt tctcatactg
     1861 cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca gcggcagttt
     1921 ttcagaccac agaatggtca tgttcggttc cggagacggc cccatagtgt acagggtgtt
     1981 caggaaacgg aagctgtttt tggtaaccag ggtacgacca tcgaggccca taccaccgat
     2041 agattcggtt gcccagattg ggtcgccaga gaacagttca tcgtattccg gagtacgcag
     2101 gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg cttcttgttc
     2161 ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg tggaggtacg
     2221 accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc cgaagtaagt
     2281 ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc cgtatttcgc
     2341 agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt cgcgcagacg
     2401 gatagtctgt tccaggttta cgccgttttc cagatcagcc tgtagagagg tgaactgagc
     2461 gtatttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt agtcaccgat
     2521 gatacggcca cggccataag catctggcag accggtcaga acaccggatt tacggcaacg
     2581 caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac ggtattcagt
     2641 gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg aaccttcgat
     2701 cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag tctgcagacc
     2761 aacaactttt tccaacgctt tattgatgta gccagcgtcg tgagaggtga tggtggaagc
     2821 aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt tccagtttaa caccttccat
     2881 tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg actcgtcacc
     2941 ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat tctgccagtc
     3001 acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg acatgtaaca
     3061 cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt aatggtgatc
     3121 gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac cgccgataat
     3181 attaccgatc gtaaccggga tcaggttatc agtgatgaag ttcatcacgg tcaggtgaga
     3241 aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga agtcgcggat
     3301 tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac cgctggcaac
     3361 aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc ggccagaata
     3421 actcatccat actgccagac ataccatcag gtttgccagg ataccaagac agacggcctc
     3481 aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc cccattgacc
     3541 atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca gtgcgccgac
     3601 caggttgcca aaatagacat ttagccagtt tttagccaac tgacaccagg tgatgcgccc
     3661 actcgcctta gcaacaacaa tcaacactgt ggaagtaaag agatcggctc cgcagacaac
     3721 acaaagaatc agccccagag agaagcaaat gccgccaacc agttttgcca tgccgaaggg
     3781 cattgtgcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg agatgaaaac
     3841 accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt tatagacacc
     3901 cgcctcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag ggttgtcagc
     3961 tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag cattatagat
     4021 gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg catggttttt
     4081 atgcaaatac ggagtaacta tttgattatc caaataaaaa taaattttaa aaattaacaa
     4141 atgagttgaa ttttttccgc atcctccgct aaaacagtta attaaaaggg agcatcaggc
     4201 gaataaagta acaatatcga tcgtatttat taaatacaaa ttaccgatat ttaaccttat
     4261 aattacaatt attttattaa tgcaaatata tgtaaagcgg ggcattaaaa aaacgccccg
     4321 taatataact cagactaatc attaagccta cattgcgtag gctatttgat tttattttgc
     4381 ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacaacg actgatgtgc
     4441 agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg taaaacgccg cttcgccgct
     4501 cattgccgca ctggcggctt ctacggcatc tgcgccgtac atgcgaacaa acgcattcag
     4561 atattgcagc ggctggcgat cttcttcctg tgccagtaat aacaacgttt gcaggcagcg
     4621 ataatagttg gcgcgttccg gactaaatac tgatgagtta aactccatcg tccattcggt
     4681 ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt ttaattcacc
     4741 gatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca gctcacgcac
     4801 gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca ggtaatcttc
     4861 tttttcccac tcgctgcctg gtagcgaaag aatcgtttca cgtaaatggc tgcccatact
     4921 gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag gcacgataat
     4981 acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt tatcttcttt
     5041 gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga aattccagtc
     5101 cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac cgctggaatc
     5161 gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga aggttggcgg
     5221 agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca cggtacgttc
     5281 cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc cgtttgcagg
     5341 attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat catatgcgaa
     5401 gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca accaccgctg ggtaacgcgc
     5461 cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc ggtttttcac
     5521 gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttgcggg tattgccagc
     5581 ggacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat aaacggtctg
     5641 attgtcggac tggcgcgtaa acggcagacc gcaaatacca cgatcttcgt taccggattg
     5701 taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg cgcgcagacg
     5761 gtcatcgagc agcccttctg gcacatcgtc attttcgctc agtgggaacc atttttcgtt
     5821 gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat cagcaaaaaa
     5881 gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg ccgctttctt
     5941 ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa tatgtacaga
     6001 ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc cgaggtctga
     6061 aagtttttgc tggaagcgag cgatggaatc ttccagagcg gcatctttgc cggggataaa
     6121 tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata cgggttttat
     6181 ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt ttagcggata
     6241 aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc atttgataaa
     6301 gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt tgtactggtt
     6361 ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca gatgtcgctg tttgaagtgt
     6421 taatcattct cacgctgggt tcagcggcgg gagatgtggc gttttatgat gatgtgccga
     6481 tggtgccggt acttatcgtc tttatcactc tggcgttgtt ataccgcctg gtaatgtgat
     6541 ggcgagctgg cctggtcgaa actcaataac tccaacatga cggaatttga gttctttatg
     6601 gagctacgat tgcgtggcgt ggagcagctg gggcaggtac gtctggcgat tctcgaaacc
     6661 aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg tgaaaccggg tttacttatt
     6721 ttacccagtg attgtacgca gcgttacaaa gtggtgccgg agtcggcgga ctatgcctgc
     6781 attcgttgta gtgaaatcat tcatatgaac gcgggggaaa aacaattatg tccgcgctgt
     6841 gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg tgacctgaca gtaaaaacat
     6901 cggctttttg ctaataatcc gagagattct tttgtgtgat gcaagccaca tttttgccct
     6961 caacggtttt actcattgcg atgtgtgtca ctgaatgata aaaccgatag ccacaggaat
     7021 aatgtattac ctgtggtcgc aatcgattga ccgcgggtta atagcaacgc aacgtggtga
     7081 ggggaaatgg ctcaaatctt caattttagt tctggtccgg caatgctacc ggcagaggtg
     7141 cttaaacagg ctcaacagga actgcgcgac tggaacggtc ttggtacgtc ggtgatggaa
     7201 gtgagtcacc gtggcaaaga gttcattcag gttgcagagg aagccgagaa ggattttcgc
     7261 gatcttctta atgtcccctc caactacaag gtattattct gccatggcgg tggtcgcggt
     7321 cagtttgctg cggtaccgct gaatattctc ggtgataaaa ccaccgcaga ttatgttgat
     7381 gccggttact gggcggcaag tgccattaaa gaagcgaaaa aatactgcac gcctaatgtc
     7441 tttgacgcca aagtgactgt tgatggtctg cgcgcggtta agccaatgcg tgaatggcaa
     7501 ctctctgata atgcggctta tatgcattac tgcccgaatg aaaccatcga cggtatcgcc
     7561 atcgacgaga cgccagactt cggcaaagat gtggtggtcg ccgccgactt ctcttcaacc
     7621 attctttccc gtccgattga cgtcagccgt tatggtgtaa tttacgctgg cgcgcagaaa
     7681 aatatcggcc cggctggcct gacaatcgtc atcgttcgtg aagatttgct gggcaaagcg
     7741 aatatcgcgt gtccgtcgat tctggattat tccatcctca acgataacgg ctccatgttt
     7801 aacacgccgc cgacatttgc ctggtatcta tctggtctgg tctttaaatg gctgaaagcg
     7861 aacggcggtg tagctgaaat ggataaaatc aatcagcaaa aagcagaact gctatatggg
     7921 gtgattgata acagcgattt ctaccgcaat gacgtggcga aagctaaccg ttcgcggatg
     7981 aacgtgccgt tccagttggc ggacagtgcg cttgacaaat tgttccttga agagtctttt
     8041 gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg gcggaatgcg cgcgtctatt
     8101 tataatgcca tgccgctgga aggcgttaaa gcgctgacag acttcatggt tgagttcgaa
     8161 cgccgtcacg gttaatgccg aaattttgct taatccccac agccagcctg tggggttttt
     8221 atttctgttg tagagagttg agttcatgga atccctgacg ttacaaccca tcgctcgtgt
     8281 cgatggcact attaatctgc ccggttccaa gagcgtttct aaccgcgctt tattgcttgc
     8341 ggcattagca cacggcaaaa cagtattaac caatctgctg gatagcgacg acgtgcgcca
     8401 tatgctgaat gcattaacag cgttagggtt aagctatacg ctttcagccg atcgtacgcg
     8461 ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa ggtgccctgg agttgttcct
     8521 cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct ctttgtctgg atagcaatga
     8581 tattgtgctg accggtgagc cgcgtatgaa agagcgcccg attggtcatc tggtggatgc
     8641 gctgcgcctg ggcggggcga agatcactta cctggaacaa gaaaattatc cgccgttgcg
     8701 tttacagggc ggctttactg gcggcaacgt tgacgttgat ggctccgttt ccagccaatt
     8761 cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa gatacggtga ttcgtattaa
     8821 aggcgatctg gtttctaaac cttatatcga catcacactc aatctgatga agacgtttgg
     8881 tgttgaaatt gaaaatcagc actatcaaca atttgtcgta aaaggcgggc agtcttatca
     8941 gtctccgggt acttatttgg tcgaaggcga tgcatcttcg gcttcttact tcctggcagc
     9001 agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt ggacgtaaca gtatgcaggg
     9061 tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg accatttgct ggggcgatga
     9121 ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat atggatatga accatattcc
     9181 tgatgcggcg atgaccattg ccacggcggc gttatttgca aaaggcacca ccacgctgcg
     9241 caatatctat aactggcgtg ttaaagagac cgatcgcctg tttgcgatgg caacagaact
     9301 gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac attcgtatca ctcctccgga
     9361 aaaactgaac tttgccgaga tcgcgacata caatgatcac cggatggcga tgtgtttctc
     9421 gctggtggcg ttgtcagata caccagtgac gattcttgat cccaaatgca cggccaaaac
     9481 atttccggat tatttcgagc agctggcgcg gattagccag gcagcctgaa tgaacaacgg
     9541 gcaataaata gccaaatctt tctttatcaa aacgtcggca cattgt
//
LOCUS       DAJVQR010000131         9440 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVQR010000131 DAJVQR010000000
VERSION     DAJVQR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            39..1154
                     /locus_tag="OQM52_003574"
     CDS             39..1154
                     /locus_tag="OQM52_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565277.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="OQM52_003575"
     CDS             1833..2051
                     /locus_tag="OQM52_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1565278.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /locus_tag="OQM52_003576"
     CDS             2269..2511
                     /locus_tag="OQM52_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565279.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OQM52_003577"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OQM52_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1565280.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OQM52_003578"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OQM52_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1565281.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OQM52_003579"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OQM52_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1565282.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OQM52_003580"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OQM52_003580"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1565283.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="OQM52_003581"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="OQM52_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1565284.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2315 c         2446 g         2368 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DAJVQR010000132         9429 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVQR010000132 DAJVQR010000000
VERSION     DAJVQR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..424)
                     /gene="xdhC"
                     /locus_tag="OQM52_003582"
     CDS             complement(<1..424)
                     /gene="xdhC"
                     /locus_tag="OQM52_003582"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1565285.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCR"
     gene            complement(421..1299)
                     /gene="xdhB"
                     /locus_tag="OQM52_003583"
     CDS             complement(421..1299)
                     /gene="xdhB"
                     /locus_tag="OQM52_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1565286.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1310..3607)
                     /gene="xdhA"
                     /locus_tag="OQM52_003584"
                     /pseudo
     CDS             complement(1310..3607)
                     /gene="xdhA"
                     /locus_tag="OQM52_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4022..4777
                     /locus_tag="OQM52_003585"
     CDS             4022..4777
                     /locus_tag="OQM52_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1565287.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4856..4929
                     /locus_tag="OQM52_003586"
     tRNA            4856..4929
                     /locus_tag="OQM52_003586"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4888..4890,aa:Gly,seq:ccc)
     gene            5064..6674
                     /locus_tag="OQM52_003587"
     CDS             5064..6674
                     /locus_tag="OQM52_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1565288.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6706..7313
                     /locus_tag="OQM52_003588"
                     /pseudo
     CDS             6706..7313
                     /locus_tag="OQM52_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8244..8990
                     /locus_tag="OQM52_003589"
     CDS             8244..8990
                     /locus_tag="OQM52_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1565289.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9288..>9429
                     /locus_tag="OQM52_003590"
     CDS             9288..>9429
                     /locus_tag="OQM52_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565290.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2404 a         2332 c         2405 g         2288 t
ORIGIN      
        1 agcgacaaag atttcccgcc agtccgcgac gaatttccgt aatggttaat ggcttctcgc
       61 gtggtttcgc cagcattgcc gtggtagcca taatcaggcc aggcgtacaa aacccgcact
      121 gcactgtgcc ggatttcgca taagcctgct gaacatgaga aagttttccg cctttcgctt
      181 caccttccag cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat aagcaactgt
      241 ctattgctgt gccgtcgacc aacaccgtac aggcaccaca ttcacccacg cagcaccctt
      301 gtttgacact tagcagtcct tgttcgcgga gtaattccga gagcggcgtg cctggtgcgg
      361 cgtgaagctg aaaaggcatc ccgttaatgg tgcattcggt ggtaattgtt tcgctgtgat
      421 tcattgcaat tttccccccg ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt
      481 ctggatgaga tgcagacgaa actctttact ggcccgccat gaagaacgcg gggcgacatc
      541 ttgcaggaca gattcgctga tagcttccag cgtttgcagg tttaatggcg cattttgtgc
      601 agtctgttcg gcatgttggc agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg
      661 taattcgctg aaattgccgt tatccagtcg gcaatgtgcg gcgcagccaa tcgttgaaat
      721 atccattgcg tcgcgcatgg catatttaaa atgcgcgctg cccgcgtgtt ctttcggctg
      781 tggcggaaaa tgaaaggcga cgaggatttc gtcatgctca agagacactt tgcccggccc
      841 ggtgtgaaag ccattaatcg ggacgaaacg aacaccgcgc ggggagtgga tctccagttt
      901 cgcgtcataa attagcgttg gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt
      961 tccaccgtag gtagcgacgt tacggatctg cggtccagca atgaacgtgg ccgcagcaca
     1021 taacgccggg agatgacgtt gagttatagg atcttctatt agctgggtaa atgtcgttgc
     1081 agagccgata cgtagcgagc catcttccgc cagcgtaatt ccccgcagct ccgccagatt
     1141 atgaatatca acaatatggc gataacggtc attgtgatgg tggagctgaa tcagtacgtc
     1201 agtgccaccg gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga tggcatcggc
     1261 aagggttgct gcgcgatggt aagaagcaaa atcaaacatg atgttatcct caaatcaatc
     1321 ctgccagatg gaactcttca tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa
     1381 caccggtagc catcttcacc gcgttacgaa tagcagcggc aacaggaatt atgggtggct
     1441 cacccagtga cttatgtccg tatgcggatt gcggctcatt gatttcgacg aacgcgcttt
     1501 ccagttgtgg cagatccggc atggtcggca ttttgtaatc cagcagattg gggttacgga
     1561 ccacgccgct tttagcatcg atgatcatct cttcaaatag cgcccagcca atgcccattc
     1621 ccattccgcc gtgtacctga ccttctgcca gcagtggatt aagaatatgc cctgaatcat
     1681 gaacgttgag gatgcggttg atggtgactt tgcacagcgc aatatcgacc gtcagatcaa
     1741 caaaggtaca gccaaacgcc ggtgggttag tggtggtttt gatggagctt tcagcagaga
     1801 gctgcccgcc gcgttcaggg tggtagaaag cgtccatcgc caaatctttt aacgacatta
     1861 acggctcttc cggtcgttca accagcacga tatggccttt tatcagggtc aaattcatcg
     1921 ctgactgatg tagcatgact gcggcgtgag cgatgatttt ctcttttaat aatagtgccg
     1981 cactgcgcag cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgcg ccgggatcga
     2041 acggcgtaac gtcggtatct tgtgttgaaa taacgcgaac gtcgctgacc ggaaccccca
     2101 cggtttctgc caccatttgc gagaagacgg tgtcggcacc ctgaccgatt tccgtcgcgc
     2161 cgctttgcac gttgatggtt ccatcctgat tcatcagaag gcgcgcgcct gctatttcta
     2221 cgccgacagg ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg ccgcggcgca
     2281 aattgccttg ctggttctgg cattctgcac ggcgtttttc ccattcaaag attttccggc
     2341 ctttttcaag acactccggc aaccctgcgc tgcaaatacg tttgcccgtg agcggattag
     2401 catctccttc gcgggcggcg ttgcgtaaac gaatttcaac aggatcaata cctaacgctg
     2461 tcgcggcgtc atcaagcata gactcaacgg caaatacgac ttgtggcgcg ccataaccac
     2521 gcatcgcacc agccgagggg aggttggtat agcaggtctt tgaactgtaa gcgtaggcac
     2581 aacgaggata aaggtaagcg actttattcc cccccgcaga agcgatggag tgcccgtgag
     2641 atgcataagc gccggtgtta gacagaacat ccagactata acctttcaag gttccgtcgc
     2701 ggttcacgcc catttgcccg tcaatagtaa aagcgtggcg ggtacgggtt gcgaggaaac
     2761 actcttcacg gctaagggaa actttcaccg gaatgccgcc aagcttgctg gtcaggaatg
     2821 ccgccattgg ctcttccagt acatcctgtt tattaccaaa accgccaccg acaaacggtt
     2881 tgatgactcg tacgcatgac cagggaatat ccagcgcctg accaaccacg cggcgaacaa
     2941 tgtgcgggat ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc atccacgcca
     3001 gcgatgttac gctttccatg tgacaatgtt gaataacggg agtctgatag tgcccctgta
     3061 cctggtagtc ggcggcatcg attgtttgtt ggacattgcc cgtcgacatc gtgctttgtt
     3121 tcagtaaatt gccgccgtta tggattggtg cagcgtcttc tgtcagcgcc gcttctggcg
     3181 tggtgataac gggtaattct tgccactcaa tgctgaccaa ttgcgccgct ttttctgccg
     3241 tgagttcatc gcgggccacg acgatggcaa cggcgtcacc atgatgacga acatggcgag
     3301 ttagcagtgc gcgatcggcg gtatcgcgct tgttttcgtc aagtgtctag gcatgcccag
     3361 ctgtagcgaa tggaatatca ggcacatctt cccaagtaaa aatcgccagt acgcccggta
     3421 aacttctggc ttgttcatca ttaatactta cggcataacc atgtgcgata gggctacgta
     3481 catatttcgc gtaacacatg cccgccataa cataatcgtc agtatatcgt gcccgcccgg
     3541 tgaccttagc aatggcatcg acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg
     3601 cttccataga aatcccctcg atttgaacat ttggttctta tggcaaagat ttagtctaag
     3661 caggcaagat ttacgccaga aacgacgata agcgttaggg gaggaaggaa gtgtgatgac
     3721 gttaaaaaaa cacagatatg atacgaaaca ggactctgaa tagggacagt gatacgtatc
     3781 aatatgagtg gcgataaaat caatgtgata aatcaggatt attacgcatc aggatgggaa
     3841 atagggtaaa aagtcaggaa cttcgatacc tctacagcga atattttgct gatgatactt
     3901 ccctttgcca aatgaaatta acctctttct ctattaccac gtttttccag aagcaagaga
     3961 ttgggtatca agaggctggc tgctatgata tggcgtcttg ttttttaagc gaggaaagat
     4021 tttgagtgcg ggacgcctga ataaaaaatc tctgggtatc gtgatgtttt tatcggttgg
     4081 actgctattg gcgggctgtt cgggtagcaa atcatccgat acaggaacgt attccggctc
     4141 cgtttacacc gtgaaacggg gggatacgct atatcgtatt tcgcgcacca cgggaaccag
     4201 cgtaaaagag ctggcgcgac tgaacggcat ttccccccct tacaccattg aagttggtca
     4261 gaaactaaaa ctgggtgggg cgaaaagtag cagtagtaca cgtaaatcaa ccgccaaatc
     4321 aacgactaaa accgcatcgg ttacaccgtc atcagcggta ccgaaatctt cctggccgcc
     4381 agtagggcaa cgttgttggt tatggccaac gacagggaaa gttatcatgc cgtattcgac
     4441 agcagatggc ggcaataaag ggattgatat ctcagctcca cggggtacac ctatttacgc
     4501 cgcgggtgca ggaaaggtgg tgtatgtggg caaccagctg cgtggctacg gtaatctcat
     4561 catgattaaa cacagtgaag attacattac ggcttacgcc cataatgaca cgatgctggt
     4621 aaataatggg caaagcgtga aggctgggca aaaaatcgcc actatgggga gcacggatgc
     4681 ggcatctgtt cgcctgcatt tccagattcg ttaccgtgca acggcaattg atccgctacg
     4741 ttacttgccg ccgcagggca gcaagccaaa atgctgatgg cgaattaatc agcagtcagc
     4801 agcgcggcac ttgctaagga gagcgtaagg tttataatgc cttacgcatc tcgaagcggg
     4861 cgtagttcaa tggtagaacg agagcttccc aagctctata cgagggttcg attcccttcg
     4921 cccgctccat gacacttctt aaagtctact aacttactga atcctaaggg atacggctta
     4981 acgccttccg atagttttac tcactgtaac caacactgtg actaactttg ttgaagtagt
     5041 gagcagacct aaggtctcag tacatgctca agtcaagaac ctacctttat cagcgtaatg
     5101 gtgtctttta catccgtctt aggatgaaga ccactagtcg cctgaccgct tcgcttcctt
     5161 ctcacaaccg ttataaacta gcgtcagtat ctttacggac caaggataga cgtaccgcta
     5221 tggctcactc acggcacatc aagtcagcac ttaaagcaat ccatgcagac aaccctaacg
     5281 cctcttatga ggagctacgg gagcacctga agaccattgt cgagtgggaa cttagtgtca
     5341 gccgtgatga cctgaacgac ccggagtcct accagcttta cgttgaccag tacgatgaca
     5401 ttaagtccaa ccttcgggaa gctgtagcga ccgagcgtct gactgtagac cagcatcgct
     5461 atattaatga cgttatcggt gtgcttaagg cctgtcagga tagactcaat ggcgatagct
     5521 ctggcctatt gtcttacctt gagcctgaga ctggcagtct aagaccgtcc gtctccctat
     5581 ctgtattggc ggaacctgaa gtccctgaac ctaaagcact gaccctcgcg tctctcattg
     5641 agcagtacga gcaggagaac gcccagaact ggaagccagc gaccctaagc gaaaaccgag
     5701 cgtcacactc tacgctaatc gagatattcg actatctgga tattcaggat gtcggcaagg
     5761 cgacccgtgc agatatgctc agagttcgtg aagtcctcca gcagctaccg aagaaccgca
     5821 agcaacgctt taagtctatg ccgctgtctg acttgctgaa ccgggagtct aagactgact
     5881 gtctggacgt cgttaccatt aacaataaat atcttatcaa gatggctgct gtgtttaagt
     5941 gggcggtacg caacgacttg atagctaaga acctgaccga aggtcttgag ctgaaagtcc
     6001 ctcagcgtaa agcctccgac gctcgtgatg cgttcagtcc tgagcaggta gggcaactac
     6061 tggtcgcagc taaagcgtac tcgcagaaga cctcaggtaa gccataccat tattacgtca
     6121 ccgctctggc tgcgattaca ggtgcgagac ttaacgaggt ggcccaactt caggttaaag
     6181 atgtcaggac cactgaggct gggaccgttt ttatccatat caacgaagac gatagcagcc
     6241 tgccgggtaa gagtatcaag aacgcccaca gtgaccgctg tgtcccgttg gtagatggtg
     6301 cttatggttt tgtattggct gactttatgt cactcgtgga agaccgtaga aagactgagg
     6361 gtgataatgc tatggtgttc aatggcctca agctaatgaa gaacggctac ggtgagcagg
     6421 ttagcaagtg gtttaaccga acgttgctgc ccaaagtcct cgctgaccgt agcggcttag
     6481 cttttcactc gtttagacat acagttgcga cccagttaaa acaacatggg gtcgagttag
     6541 cgtatgctca ggcgattatg ggtcatagct cagggtctat aacttacgac cgttacgcga
     6601 aagaggtcga ggtggaaacg ctgaaagaaa aattagcgga gtcattgtca gtaaaaaaaa
     6661 tagacggaaa gtaatcgcat agggatagcg gaatgtgagg acgtaatggt caaagaattt
     6721 ttagttagca aagaaaataa gccttcatat gaatgtatga atactgctga aacttgtttg
     6781 gggctggctg gaatggcttc ggaagattgg caacgtgagc atcattgccg cacaggtata
     6841 atatttactg cttttgctat tgaggcaatg ttcatcttct atagaaaaca ggttgacccc
     6901 ggctacgata agacgcagaa agagtgcaga aagacaatgc acaagaatac gttaaaattg
     6961 tgcggtataa acaattatat ggggacaaaa ccttaccaaa ttatcaaaga gtgtcttgag
     7021 gtaagagacg ctattgctca cggcgattcg tatacttcga gctttaattt ctcggcggat
     7081 catttggata accaagacga tattgcaaaa gtgtcctggc ggtaaactct gaacaatttc
     7141 ggggattaac tgttgagcgc cttgcaaaat acattgaaat ggcaaagcat attgaccatg
     7201 agatttctga taatggttac aggccttctg aaggttacct tccagcagcc gagcgcagaa
     7261 gtctgatgtt agcttttggc gtcagtggtg tgtcaatgtg gcccagtgct tgagcgataa
     7321 tacacctcac tatagaggaa tggaccctta actatcacta taggggagag accctaagtc
     7381 cccaccttaa tagcagtcac gacccataca cacattcatt aagtctcagg cttccggtag
     7441 cggaccgatt ggaaaccatc cgcagacgcc tagtgttaaa tcaccttggc ctgagaccat
     7501 tccttttaac gtaagctatc ttcccttttg acaggcgggg agcagtagac cacatccgac
     7561 tttaagttgg ctctgtgata atcgctgcgc ggataactga agaaccaaga caccattact
     7621 gaggtctgcc atccatttgt ggacaggcgg aaactcaaag aggttagatc agtggtgtct
     7681 gaggttggag ctttgagtct tgaccagcag gctggaggag accatgagtt tatcttgtta
     7741 tggattgtgt tatcacaatt ccaataagaa gaactttaag tccagcctcc actcccgcca
     7801 acccgaagta aaattatggt cgctatgatg tggtctccat gcttcgggtc ttactggaag
     7861 tactgaacac tttcgcgtca agttctccga agttcattcc tgtttgacta aactatcact
     7921 atagggacca tatctcgcct ctgacctcct gagagacggc aagcgagaaa ccgtggtcca
     7981 cgctagcaag ctagccacca gcctaaggca agacagcctc tggtcagcct gtgtgttctt
     8041 tggtctcgtg acttaagtct atcaccacac acgctcggac atgctgtcga accttcatct
     8101 ctatggtcat cgcagaccac taaccccaat tacctcaagt cattctgttt gcgtcctgtt
     8161 gcctgctggt ctccctttac gtgggggctt gagtaggtca gtggggtgcg acttcagggt
     8221 gcaaacaaaa ggagtacacg taaatgtccc aacaagaaat cagcatcatt aacctcgacc
     8281 agcttgtgtc catgacctca gttgaaattg ctgagctgac aggaaaagaa cataaacacg
     8341 ttttacgaga tattcgtaat atggtcgaag agcttaacgg ggccaaaact gagcactgct
     8401 caactttgag cagcgagctt aacgggtcca aatttgggct cgttggcgag gaagtataca
     8461 aggacgcgaa aggtgagagc cgaacaatgt atcgtctcga ccgtaagcat actttcattc
     8521 tggtcgctgg ctattccgtt cacctccgcg ccaagtgtta cgaccatatt cagacgttag
     8581 agcgtcgagt ccttcaactg gaagaccaga agaagcgagc agccattcag tccgctaacc
     8641 gccgaggcgt gacttggggc gactactgca aaacctatgg tctgcccgct cagaagctaa
     8701 tgaccgcact tctccagcat cgtggcctct ttcgtaagaa cccaatcagt aacgagtggt
     8761 ctgtaaatcc gaagtattcc gactgctttc gtatcattaa gccgtcagac cagaagttct
     8821 cagcaggtgg gtataacttc cggttcaacg ctaaggggct ggaggtcttc ggtaagcctg
     8881 agatggttga caagatgcga ggcatcctta ttgcattcac tggcaccgac cagcagaagc
     8941 aagagcacct cctgaagctg gcacaatccg gtaaggtgga gggaatctaa gatgatgatt
     9001 gacccgcgca cacctgaggg gcgcatgact ctccgttacc gtggctaccg gactgaagtc
     9061 ctcctcagag agcttggtct agacccggaa gatgagaccc gccagcacca atcccgagac
     9121 gagctaatag cccaactggt cgctatgaaa ctgccgctta accgttagta gctcacctac
     9181 tcaataccta atttaaacac cttttgagat gaccgatgcg gggagacccg taggccttac
     9241 tgtgcctgcg ataccgatag tctcaactct caagaggaga attacctatg atgttcaaca
     9301 acaacaactg gaagctgtca gtcaccgaca tcaaccttta tgaaaacact gtgagtctgg
     9361 acggtcagtc ctatccgttg tcgctcgcta tcaagaccct cattccgggc tacctgtcag
     9421 gcttaccgt
//
LOCUS       DAJVQR010000133         9412 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVQR010000133 DAJVQR010000000
VERSION     DAJVQR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="OQM52_003591"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="OQM52_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1565291.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="OQM52_003592"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="OQM52_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1565292.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="OQM52_003593"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="OQM52_003593"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS1565293.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OQM52_003594"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OQM52_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS1565294.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="OQM52_003595"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="OQM52_003595"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS1565295.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="OQM52_003596"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="OQM52_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS1565296.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="OQM52_003597"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="OQM52_003597"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCS1565297.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2477 c         2598 g         2288 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAJVQR010000134         9242 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVQR010000134 DAJVQR010000000
VERSION     DAJVQR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQM52_003598"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQM52_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1565298.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQM52_003599"
     CDS             1828..3147
                     /locus_tag="OQM52_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1565299.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OQM52_003600"
     CDS             3445..3486
                     /locus_tag="OQM52_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565300.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OQM52_003601"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OQM52_003601"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS1565301.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OQM52_003602"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OQM52_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OQM52_003603"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OQM52_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OQM52_003604"
     CDS             5815..6711
                     /locus_tag="OQM52_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1565302.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OQM52_003605"
     CDS             6708..7682
                     /locus_tag="OQM52_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1565303.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OQM52_003606"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OQM52_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OQM52_003607"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OQM52_003607"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1565304.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 ag
//
LOCUS       DAJVQR010000135         9175 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVQR010000135 DAJVQR010000000
VERSION     DAJVQR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            388..1083
                     /gene="minC"
                     /locus_tag="OQM52_003608"
     CDS             388..1083
                     /gene="minC"
                     /locus_tag="OQM52_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1565305.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1107..1919
                     /gene="minD"
                     /locus_tag="OQM52_003609"
     CDS             1107..1919
                     /gene="minD"
                     /locus_tag="OQM52_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1565306.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1923..2189
                     /gene="minE"
                     /locus_tag="OQM52_003610"
     CDS             1923..2189
                     /gene="minE"
                     /locus_tag="OQM52_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1565307.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2561..2905)
                     /locus_tag="OQM52_003611"
                     /pseudo
     CDS             complement(2561..2905)
                     /locus_tag="OQM52_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2940..3059)
                     /locus_tag="OQM52_003612"
     CDS             complement(2940..3059)
                     /locus_tag="OQM52_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1565308.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3020..3205)
                     /locus_tag="OQM52_003613"
     CDS             complement(3020..3205)
                     /locus_tag="OQM52_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565309.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3306..3479
                     /locus_tag="OQM52_003614"
     CDS             3306..3479
                     /locus_tag="OQM52_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565310.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3481..3825
                     /locus_tag="OQM52_003615"
     CDS             3481..3825
                     /locus_tag="OQM52_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1565311.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3829..4164
                     /locus_tag="OQM52_003616"
     CDS             3829..4164
                     /locus_tag="OQM52_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1565312.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4221..6542)
                     /locus_tag="OQM52_003617"
     CDS             complement(4221..6542)
                     /locus_tag="OQM52_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1565313.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6659..6868)
                     /locus_tag="OQM52_003618"
     CDS             complement(6659..6868)
                     /locus_tag="OQM52_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565314.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7619..9142)
                     /locus_tag="OQM52_003619"
     CDS             complement(7619..9142)
                     /locus_tag="OQM52_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1565315.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2567 a         2119 c         2020 g         2469 t
ORIGIN      
        1 gagcaattct gtggcggata ttaagataac gggtatctgt tctttaatga tgctgcaaaa
       61 aatggaaagt taagaaagcc tggcgtggtg aaaaaataca cttgcctcaa tactggatat
      121 ttaaacaggt ctggagatgc tgtcacgatt agggtgcgga aaaaggaagc gtaaataaga
      181 agaatcaaaa gtacgacggc aatgggttga ttgacaaggg tattttttaa gctatgaatc
      241 agcgccattt atcacagaat agacttttac tctgaataaa tgggagggtg acttgcctca
      301 atataatcca gactataaca tgccttatag tcttcggaac atcatcgcgc gctggcgatg
      361 attaatagct aattgagtaa ggccaggatg tcaaacactc caatcgagct taaaggcagt
      421 agcttcactt tatctgtggt tcatctgcat gaggcagaac ctaaggttat ccatcaggcg
      481 ctgaaagaca aaatcgctca ggcccccaca tttttaaaac atgcccccgt tgtactcaac
      541 gtcagtgcac tggaagaccc ggtaaactgg tcagcgatgc ataaggcggt ttcggcaacc
      601 ggtttgcggg ttattggcgt aagtggctgc aaagatgcgc aacttaaagc cgaaattgaa
      661 aaaatggggc tgcctatcct gacggaagga aaagaaaaag cgccacgtcc agctcccgca
      721 ccgcaggctc cagcgcaaaa tacaatgccg gtcacaaaaa cgcgtttaat agataccccg
      781 gtgcgttccg gtcagcgtat ttatgctcca caatgtgatc tgattgttac aagccacgtt
      841 agcgctgggg ccgaattgat tgccgatggg aacattcatg tctatggcat gatgcgcggt
      901 cgtgcgctgg caggggcaag tggtgaccgg gaaacgcaaa tattttgtac gaacctgatg
      961 gcggaattgg tgtccatcgc aggtgaatac tggctgagtg atcaaatccc agcagaattt
     1021 tatggcaaag cggcgcgact gcagttagtc gaaaacgctt tgaccgttca accgttaaat
     1081 tgatcccttt ttaacaagga atttctatgg cacgcattat tgttgttact tcgggtaaag
     1141 ggggtgttgg taagacaacc tccagcgcgg ccatcgccac tggtttggcc cagaagggaa
     1201 agaaaactgt cgtgatagat tttgatatcg gcctgcgtaa tctcgacctg attatgggtt
     1261 gcgaacgccg ggtcgtttac gatttcgtca acgtcattca gggcgatgca acgctaaatc
     1321 aggcgttaat taaagataag cgtactgaaa atctctatat tcttccggca tcgcaaacac
     1381 gcgataaaga tgccctcacc cgtgaagggg tcgccaaagt tcttgatgat ctgaaagcga
     1441 tggattttga atttatcgtt tgtgactccc cggcagggat tgaaaccggt gcgttaatgg
     1501 cactctattt tgcagacgaa gccattatta ccaccaaccc ggaagtctcc tcagtacgcg
     1561 actctgaccg tattttaggc attctggcgt caaaatcacg ccgcgcagaa aatggcgaag
     1621 agcctattaa agagcacctg ctgttaacgc gctataaccc aggccgcgta agcagaggtg
     1681 acatgctgag catggaagat gtgctggaga tcctgcgcat caaactcgtc ggcgtgatcc
     1741 cagaggatca atcagtattg cgcgcctcta accagggtga accggtcatt ctcgacatta
     1801 acgccgatgc gggtaaagcc tacgcagata ccgtagaacg tctgttggga gaagaacgtc
     1861 ctttccgctt cattgaagaa gagaagaaag gcttcctcaa acgcttgttc ggaggataag
     1921 ttatggcatt actcgatttc tttctctcgc ggaagaagaa cacagccaac attgcaaaag
     1981 aacggctgca gattattgtt gctgaacgcc gtcgcagcga tgcagaaccg cattatctgc
     2041 cgcagttgcg taaagatatt cttgaggtca tttgtaaata cgtacaaatt gatcctgaga
     2101 tggtaaccgt acagcttgag caaaaagatg gcgatatttc tattcttgag ctgaacgtga
     2161 ccttaccgga agcagaagag ctgaaataag cccgctgtaa aaacgcattt atcttcaagg
     2221 cagagtcatt tctgccttga gtttttcatc cctctcatcc acgttgtggt aaagcggcga
     2281 gtattcttgc tgatactcct cattgctatt tccacctccc cctttacacc ttaaggctgt
     2341 tcccacgcac agaacaaaat gtttatgcct ggttgagtaa ataaccatgt tattagtgtg
     2401 tatatactgg gagatgataa ccccgcaatt tcattattca ttgttctccc ataggatgag
     2461 gtttcccgcc gtaactggcg ggcttttttt gccccagaat tttctctttc aacatcctgt
     2521 aaacgaaaac tgcgccgaag cgcagtttaa tcgttgatgg tcagaacgac cagttcacac
     2581 cagccaccgc gttccacggg gactccacac cggcaccatg gctatacccc accccgaaat
     2641 gcccgcttaa cgtactgctg aatgaggctt taatacctgc ctggtatatt ccacgtctgc
     2701 ccgacaaatc attgacaaaa ttaccgtcac tattcacttt cacccgatta tcatcgacaa
     2761 attctttgcg cacagccgcc ttcaggcagg gttcaatttc cataccgttc cccagacgca
     2821 tgttgtaact cagcgttgca cccagttcac gatatatact gcgggtatcg actgatttcg
     2881 attccatgcc attggataaa tgatagtagc gtctaagtga cctaagctct gctgcaacat
     2941 cagcccgcag gcaccagacc aggggattca tcctgaagag acagcgcagg tgtattgtgt
     3001 tcaccgctca ccaaagacat catgatgaaa tgatgatatc ccgcataaga aagaggcatt
     3061 ttttacacgc agtgcgctga agtgtagttg gataaaaaat cactcttttc agataatact
     3121 gtcgtacgct tatctttaga caatgaggcc gtcggaaaaa taaaatgact aatgtaatcg
     3181 ttgctgatga atatcttctg ctcacataaa aatcacacaa taatattgat aaatcagatt
     3241 atcttacctt tacaacattg cgttaacagt caatgacact tcttgaataa aaatattgtc
     3301 aatcaatgag ttattcctca tttaagatta ttctcattca cgtaaaaaac ataattccta
     3361 tcatcacagc cacgctcatt ttgaattatc tgaataatag cgagcgttct ttagttaagc
     3421 agatcttaat tgaagacgaa attattgtgt gtgcaactta cttaataccg gacatctaag
     3481 ttgaagaaaa aaatacttgc cttcgggctt atctcagcat tgttctgctc cacgccagcg
     3541 atagcggata tgaaccgcac cacgaaaggt gcattgctag gcgcaggcgt tggtttactt
     3601 actggcaatg gtgttaatgg cgtactgaaa ggtgcagctg ttggcgctgg tgttagtgca
     3661 gtaacagaaa aaggccgcga cggtaaaaat gcgcgtaaag gtgcaaaagt gggtgccgcc
     3721 gttggtgctg tcactggcgt tctgaccggc aatggcctcg aaggggcgat taaaggtgcc
     3781 gtgattggcg gtacgggtgg tgcaattctg ggtaaaatga aataaggaat gatgatgaaa
     3841 aaatttgctt tactggcggg cctgtttgtt ttcgcaccaa tgacctgggc gcaggactac
     3901 aatattaaaa acggtttgcc gtcagaaacc tatattactt gcgcagaagc caacgaaatg
     3961 gcaaaaactg acagcgcaca ggttgcagaa attgttgcgg taatgggtaa tgccagcgtt
     4021 gccagccgtg atttaaaaat tgagcaatct ccggaactga gcgccaaagt ggtagaaaaa
     4081 ctgaatcagg tttgcgctaa agacccgcaa atgctgttga tcacggcaat tgatgacacc
     4141 atgcgtgcta tcggcaaaaa ataatatttc atatgtaccg cgtcgatgaa tacgacgcgg
     4201 ttttttagct aacggcggga tcagaactta tatttcaggc ccaccatcac tgcggtgtca
     4261 ttgtagccat tatcacccag ctgcacgccc acattccccc agacgctggc cgctggattc
     4321 aggttgccat ttacgccaag tttaatttcc cccaaattac tcaccccatc ttgttcgact
     4381 ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatccagtt catttcaaca
     4441 aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg aataaaggtg
     4501 cggaagccga gtcgggtctg gatgttgtta tttgcgtcat tctcaatacg tgagccattg
     4561 gcttccgtgt gcgcattatg atcgacgccc atccagatag cctgtgcctg tggctggaag
     4621 atccacgtat atttcgccgc ttccagacca aaggatttat tcagggcaaa accgtaaccc
     4681 gcttccagcg agacggttgc acctttcgcg ctataggatt cataggctaa ttcatcccct
     4741 ttcacggagt ttttaaacca gctatattgc gcccaggagt caatgtatgc gcctttttta
     4801 ctgatgtcat cggcaaacca agtggcatac agccctgcgc tatagcctct gacactccct
     4861 ttcgaacgat aatccgacac gctggaatga gttaagttgt agtcgcgggc ataaccagcc
     4921 atcactccca aacgccaact gtcactatcg gtaaaaccac cggttaacag atcgccccct
     4981 aactgcgaga cgtagcgatg cgaggtcgtt ctcaactgtc cgttgctgtc acgccaggca
     5041 ttatgcccgc caatttgacg tagccaaagt cggcttgaac gctcctgttc agttacagga
     5101 tcgatgtagc gcgtttcacc cgcacggtcg ttcagacgca taacaaaaag agtgttagcg
     5161 gctgccaggt tggcgacgta acttccagct tcggggcgca ggacgggggt aggcgcaaca
     5221 gtaggcgctg aacttcctcc ggagtttgtt tcaccgccgc tcgttgcctg gaagttagtc
     5281 agataccagt ttttattggt cgcttcggta ttacctcggg ttaatgaata ttcgtaggca
     5341 ccggcgaaaa tcctcgaatc cttaataaat tccccatttg attccccctc aacgctgata
     5401 atctctatcc catttaacgt gtaagctccg ctgcctcctg cattggtaac tttaactcgg
     5461 gttgttccag aggtatttcc attaataacg actttatccg tagctgaatt atcatcatcc
     5521 aattcagttc tgagatgcaa tacgccatta ttaccaacgt aattttcagt tattgttaat
     5581 cttgttggtt cgacatcccg tccatctgcc cgggaaatat agaccgtact attatcaacc
     5641 gtgaggttgc tcaccgttga gtcagcttta atatcccagg tactattatc tgaaagcgac
     5701 agataggtat gagtgttatc atctgttgaa atattagctg aacccgttat ttttgaatta
     5761 gttgcatcga cggtcaaatg acctttaata ttattgataa aaacatctgc agccgatgat
     5821 acctcactat ccgtaatggt cacgttactc gtctcatctt tatggtagag gatttcattt
     5881 gctgaccgta gtgagctacc agataaaata tctaccgttg cttcctgacc atttatggaa
     5941 gactccagca caattccaga cccctgatgc gcgactgcag atgtattttt cagcgtcacc
     6001 gtgccgccat tataaatttt agcaatcgcg gcgtcaggac ccgttgtata aatttctccc
     6061 tggttaaatg aaactgtccc ctggcgggca gcgatggcat gagcattgtc cccatgcgtg
     6121 gtgatatcaa catcggttaa tgtaacgctg gcattggtat aatcaagcgt gtcaccagcc
     6181 ttgacgacag tgccattagc gtacgcggca taactttctg ttccgtgggt tgtgatctcc
     6241 ccttttgcca tatccagttt tgctatggca ttattttcat tacttcctgt atcggcagta
     6301 ctttcttcat caagagaaga tacacacgca cacgcgcttc cgggagatgc acacgcaaga
     6361 acgccgactg cgtgattacc cgcagtaaca ataaatgtat catctccgtc agaaaaagag
     6421 ggggtaaccg aaccgtcaga gttgactctt attatggtgt cagtattcag gtttccactc
     6481 gtttgactta gcaaaccaat tgagtaatcc cccgttgtga caatcgtgtt tttaccctgc
     6541 aaaataacag caccgccaaa ttcacttacg attgcgctag ctgttaatgc tgggcttaga
     6601 ggactattgt cagaaggata taaaccgccc gttgttacaa tgacgccatt gttaatggtt
     6661 aacttactac cctctcctgc aacggatacc cgtaacttcc attacccgtt gttataacta
     6721 gcgcattatc aatatctttt acttttccat cttttattgc cacagcatgt ttatattcat
     6781 cgtgctgaat aaactgttca gcatatgcat tacccaccgc cagagcaata agtcccaaac
     6841 taaacccggg gagttttttc agtttcatag attaactatc cattcattgg attaacaaac
     6901 attcctggtc aaataaccta tgagctcaga ttaccaaaac ggagggtaaa gacaaaaaca
     6961 aaagcacaaa aaatttaaat agataaacag cgacaatata taaatgataa ataagtcggc
     7021 attatcattt ttcaccttat tttcatgaca ttgatcactt tggggtgact attttgttta
     7081 acaaataact cttcgacatc aaaattatta atagttgcca tggtaattat ttcggtgata
     7141 tataaaaaca actgtgataa caccaaggtt gacaactatt tgcgcaataa ttcctttcct
     7201 tcaatgatcc aggcgttcac tgccagcaac ccattcagcc gcatactgac agtgagaaac
     7261 tgaaagatta ttgctgatcc ctgtggctaa cgagtctttc tcttaaatcg attaaccaga
     7321 ttgatcgcca gccaaacaag ggcattaagc aaatttatcg tcacaagcac ttctcccagc
     7381 ccggtactgg gttttgcaat aaggatgacc aggagggtga tcagcataaa tgcaatggaa
     7441 aagatgatat agagagtgaa ataatccaga ttattactat tattcatgct actaatggca
     7501 atcctgtcaa aactttcaaa caattatagc aaacaaaata atgaacaata gtaacaagag
     7561 gcaacgagct aattagctgt ctataattac accgcactat ggccttactg atcaattttc
     7621 actcaaccac aaccttcacc ttcggtttag aaaggataat tttgaccagg tcgatgtatg
     7681 taacaggttt atagaaataa taaccctgct gaaatgtgat attattttgg ttcagatagc
     7741 caagttgttc tttcgtttcg acaccttcag cgacgatact gatcgaaagt ttacgcgcca
     7801 gatccaatac acaatccaga attcgggttg attctgggtc tgcgtttacg cggccaacga
     7861 aactgtgatc aattttgata taatcaatat gcaggtcatg aagataagaa agccctgagt
     7921 agccagtacc gaaatcatcc agcgcgatga caaaaccatt ttcatgcaga atgttcaacc
     7981 gctgaaccag actttcatca acattcaatg gctcacgctc ggtgacttcc agaacaaggt
     8041 ttaaatcgcg gcgggtaaaa ctgtcacgga aatttaaaca ctcgtcgaca aacgtcggcg
     8101 aaataatatg cgaagcgcta aaattaattc caatatgaaa accttccggc agtttactcg
     8161 cgatagcgtt catctgtctg gcaacctgat tcatcaggct ttgtgtaagc ggaacgatta
     8221 atccggattt ttcagcaagt ggaataaatg ccgcgggtga aatatatcca ccgtgaggtt
     8281 gtttccagcg ggctaacacc tcaactcccc gcaatgtccc ttcccgacca tttaccacag
     8341 gttggtaaaa agggacgatc tccccattat ttattgcccg tcgcaggatc tcttcaggcg
     8401 ttgcgctttt attgaaatat ctatcaagca ggaaggcagc ggcgctggcg attaacagaa
     8461 taaatattaa aatcccaaag cctttatcga taagtctgta gaagctaaag agtggcggcg
     8521 ggttaaagat aatagtgaaa tgatattttg tggagtgaac cttctcggta tagggataat
     8581 gtccattaag cgtcatcaca tcagccgttg gtccaataat gtcatccgcc acgcgtaata
     8641 cataccttac ccctttcaag ggtacattaa gcgccccgcg aatatgttga tcacttatcg
     8701 taaccaaaat gcgcgtatct gcaaattggc tctgatagag caggataggt aatctattaa
     8761 cggtgtcgat tgccggagcc aacagtaaat tactgtccgg gacaacagga atacgagaca
     8821 gcaggacccg attcccaggc agggatgtac accagactat tccctgtttg agaatgatga
     8881 ttgtgcgcag gtgaggtttc agagctgctt cagtgccaag ctgatattgc tcctcgaggt
     8941 cgcattcctt cccggcaatg ttaaccgctg tttgagtcgc ctgtgacgct tcttcaagta
     9001 tatgattgat attgttggcg gcatatcttg ctcccgccag gtactctgct tttgcggaat
     9061 accagagctg gatgttcaaa atagcgacac cggtaataaa caagcatatc gcaacgagga
     9121 tgggtatgag tgtattgcgc atttgcttgt cccggccaaa gggtttaata catga
//
LOCUS       DAJVQR010000136         9171 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVQR010000136 DAJVQR010000000
VERSION     DAJVQR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..894)
                     /locus_tag="OQM52_003620"
     CDS             complement(<1..894)
                     /locus_tag="OQM52_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCS1565316.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            954..1232
                     /locus_tag="OQM52_003621"
     CDS             954..1232
                     /locus_tag="OQM52_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565317.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OQM52_003622"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OQM52_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1565318.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OQM52_003623"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OQM52_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1565319.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="OQM52_003624"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="OQM52_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1565320.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="OQM52_003625"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="OQM52_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1565321.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="OQM52_003626"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="OQM52_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="OQM52_003627"
     CDS             complement(4912..5442)
                     /locus_tag="OQM52_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1565322.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OQM52_003628"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OQM52_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1565323.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="OQM52_003629"
     CDS             complement(6411..7634)
                     /locus_tag="OQM52_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565324.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENSIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8215..>9171)
                     /locus_tag="OQM52_003630"
     CDS             complement(8215..>9171)
                     /locus_tag="OQM52_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1565325.1"
                     /translation="VFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRGYLL
                     AALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFGWKV
                     TLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCC
                     SPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFI
                     PSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGS
                     AGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2675 a         1822 c         1920 g         2754 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg ttgttcatat
     6841 ttatgtagtt tggggatgga attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt tttattatcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt gtgtataaaa
     7801 aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa cgctcatttt
     7861 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
     7921 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
     7981 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
     8041 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
     8101 ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta tgattttcta
     8161 cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat ctatttaaaa
     8221 tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg tcaggaatgc
     8281 agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca ggcttgcgcc
     8341 ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg tcatcagaac
     8401 agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga caacggcgct
     8461 taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg ctgagggaat
     8521 aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca gtacatcttt
     8581 aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta ctacagcttt
     8641 ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac tacaacaact
     8701 cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac aacaatttgc
     8761 tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca ggataatact
     8821 ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt tccaaccaaa
     8881 ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa ataaaaaagt
     8941 gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg gtattgtcga
     9001 gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc ctctcccttt
     9061 tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta tcaggctgac
     9121 accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca c
//
LOCUS       DAJVQR010000137         9166 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVQR010000137 DAJVQR010000000
VERSION     DAJVQR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..924
                     /locus_tag="OQM52_003631"
     CDS             <1..924
                     /locus_tag="OQM52_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1565326.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYITWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="OQM52_003632"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="OQM52_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1565327.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OQM52_003633"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OQM52_003633"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1565328.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="OQM52_003634"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="OQM52_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1565329.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="OQM52_003635"
     CDS             4447..4806
                     /locus_tag="OQM52_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1565330.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OQM52_003636"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OQM52_003636"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1565331.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="OQM52_003637"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="OQM52_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS1565332.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="OQM52_003638"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="OQM52_003638"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1565333.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2219 c         2474 g         2379 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgtc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tttacattac gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccatgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt ggtaggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaagcaa cagaatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggt
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgctgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctttactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 taaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggtgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc gacgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
     9121 aatgcttgac tctgtagcgg gaaagcgtat tatgtacacc ccgcgc
//
LOCUS       DAJVQR010000138         9134 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVQR010000138 DAJVQR010000000
VERSION     DAJVQR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..594)
                     /locus_tag="OQM52_003639"
     CDS             complement(<1..594)
                     /locus_tag="OQM52_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCS1565334.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQN"
     gene            complement(661..1386)
                     /gene="fimC"
                     /locus_tag="OQM52_003640"
     CDS             complement(661..1386)
                     /gene="fimC"
                     /locus_tag="OQM52_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1565335.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1423..1962)
                     /locus_tag="OQM52_003641"
     CDS             complement(1423..1962)
                     /locus_tag="OQM52_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1565336.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="OQM52_003642"
     CDS             complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="OQM52_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1565337.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3056..3652)
                     /gene="fimE"
                     /locus_tag="OQM52_003643"
     CDS             complement(3056..3652)
                     /gene="fimE"
                     /locus_tag="OQM52_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1565338.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4130..4732)
                     /gene="fimB"
                     /locus_tag="OQM52_003644"
                     /pseudo
     CDS             complement(4130..4732)
                     /gene="fimB"
                     /locus_tag="OQM52_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6186..6902
                     /gene="nanC"
                     /locus_tag="OQM52_003645"
     CDS             6186..6902
                     /gene="nanC"
                     /locus_tag="OQM52_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1565339.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6922..8028
                     /gene="nanM"
                     /locus_tag="OQM52_003646"
     CDS             6922..8028
                     /gene="nanM"
                     /locus_tag="OQM52_003646"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1565340.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8093..9073
                     /locus_tag="OQM52_003647"
     CDS             8093..9073
                     /locus_tag="OQM52_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1565341.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2678 a         1989 c         1916 g         2551 t
ORIGIN      
        1 attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc
       61 ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt
      121 caggttcagt cgctgctgac caacatccag atgcgcagta gcgtcctgga ccattgtggt
      181 taatggcaca caggcatcat ccgccagcag attcataccg gcgacagaag ccgtattcag
      241 ccccatactg gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc
      301 cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacgcg
      361 atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg
      421 gggatcatcc gctaaaaagc gcagattaaa atagaggtcg gcagatgaca aaggtgcctg
      481 tgcggcaaaa gcacaggcga caacgagtcg gacaaaaaaa ccagccaaac gatgcttacg
      541 aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg acattattcc
      601 tccggtttaa aatcacccgg cagtcaattc tttttattca ggcgaaaaat tccccctgcg
      661 ttattccatt acgcccgtca ttttgggggt aagtgcgcca taatcattta ttgttcggta
      721 agtaatattg cttcctgcat cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac
      781 caatgcattt tcaagaaccc gggttccggc attcaactct gttaccgtca ggtaataggg
      841 tgtcgggtta atcagcgtca gagaattcgc gctacgacga aatcttaatt tttctgcggc
      901 ctgatcgggt ggcaacgcta atttagccgg gcgatagtac agtttaatgc ggctgataat
      961 tgcgagctgt agcatattct cagtcaattt tgatttatcc attgacggaa tcgctttaac
     1021 gttcatccag aataaacttt cccggtcctg tggcaattgg ttatttgttg catcaagaat
     1081 acgtaaggta ttctcttttt ttcccttcat cgcaaacaga ggaggcgtca cgataaaacg
     1141 accatccttt acaccatcgg cattttccac ccatgattga attaaatagg tactattttc
     1201 atcattattt gtcacggcaa gttgcacttg tttttgccct gccggataaa ttacgcgagt
     1261 cgcacctaag gccactcctg cttcagcgcg tccggcaacc atcattgcca tgaacatcag
     1321 gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga cgtttttatt
     1381 actcactgtc ctgttcctgt tatcacatta tctactgaac aattactgat aggttaaaga
     1441 gaaccaggcc tgggcattgg cgatgccgcc agtaacccga cgcccggtag cacgatattt
     1501 ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg ctggaggacg
     1561 attaatcggt acgaggtttc cttcatcatc aaacaacgct acaccaatat tggaggctat
     1621 ccctggcccc tctcccacgg agagcacatc cggattttta ccatccgcga caccgtgaaa
     1681 cgccacaccc acacgttcac tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg
     1741 caccggtgcg ctatcttccc ctgccgcatg aaaccggtta ctgctgattt gccccatatt
     1801 gaccgtcatt tgtttatcac cggcttcaat ccggcaagtt tccgcaataa tgacgccctg
     1861 aaattgcata tttccgccgg gcagcgtggt attccattta tttccggcca gagcaaacat
     1921 tggcagcaac gacgctaaca gaaataatct cttccttttc atcgttattt ttatcgcaca
     1981 agggtgggca tccctgcccg taatgacgtc cttgaacctg ggtaggttat tgatactgaa
     2041 ccttgaaggt tgcatccgca ttagctgcac ccggggttgc ctcgccgatt gcataataac
     2101 gcgcctggaa cggaatggtg ttggtaccgt tattcagggt tgtttgtgca ctaaatgtcg
     2161 caccgtccag cgtcagagca ttgcctgttc tgtctaggat ctgcacgcca acgtttgttg
     2221 cactacctgc agcagaactc tgcagagcca gtacatcagt acgcgtagca tcaattgccg
     2281 tacctaagaa ggcaacagcg gcttttgtgg caacagtggt atcgcaatca ttcagctgaa
     2341 tgttaaaacc aacggcagag ctggttgctc cagcctgctt caggctagcg gtacgaacct
     2401 gtcccaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa
     2461 cttccccttt aaagtgaatg gtcccaccat ttaccgtcgt agtatcggcc agagccgctg
     2521 cggaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt ttcatgctgc
     2581 tttcctttaa aaaaactatt tctaaatcga catgggcagt cgttctgtac actttgtttt
     2641 gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa acatttgggg
     2701 ccattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat ttgttggctt
     2761 aatattctat tattatcttt atttatagat ttttatattg catgaggtgg tttttggaga
     2821 gaagaatgag gaagatgcgt cgagccacag aaacgttagc tttacatata gcggaggtga
     2881 tgtgaaatta atttacaaga gaaataattt acatatcaaa tagttagatg ctttttgtcg
     2941 ttttttaata tttttatgct tgagaaaaaa tacgtaactt atttatgata tggacagttt
     3001 ggccccaatt gtcttgtgtt tatttgtttt ttaactttat tatcaataag ttaaatcaaa
     3061 cctcttctct ttttaatttt tcgtttatga gattatttct ttcccataat ccggcaaaac
     3121 gagcagcatt actggcggta taacgcacag tatggcgaat atttcgatgc ccgagataat
     3181 cctgaattaa acgggtatct gcaccacgct ccgccagttc ataaccgcaa gcatgcctta
     3241 acatatgagg atgagtctgc gttacggttc cagcttcaat accggcatcg cgaataatgc
     3301 gataggcctg ctggcgagaa agccgactcc cgcggcgaga aataaatata gcgtcggtcc
     3361 ggtcagcgcc tttccagtta gcacgttcct gggtccagcg ttccacggct tcacgctcat
     3421 caaaacgtaa cgggtgaacg gtagaaaatc cgttcttcag tcggcgaata tttattcgac
     3481 cttcattaag gtcaaggtcc tgataatgca gatcaagcag ttcactaata cgcatcccat
     3541 gccgatatgc caacagaata agacaataat ctctggctcc cgttgccccg taacaaaccg
     3601 cctgcatcat ggcctgaact tctttaccgg taagataacg acgtttactc acaatagtag
     3661 tactcctaac tgagatatat ttgaatgaat acctatagga aacctcaatc ggtcaaaatt
     3721 agcccaatag ataaaaagat aaccacacac gaagttatgt ggctatcagt caattacatt
     3781 aaacatcata caaaaataaa atatcactga tgtgacaaca tctattttca ttgatttgat
     3841 tcaatataac aatagattac agaggattaa cttccagtta cttaaaaaat aaagaaaaaa
     3901 gtgtggaaac aggagaattt aagtgatgtg taaacttatg aatgattttg tttatttatt
     3961 ttgaagaaca taaatcatta gattttgtat gggtatggtt aacttaatgt ttattatgat
     4021 aattaagaat cacacttaca ataacatcaa ttaataaatg gttaatcatt tttgatatat
     4081 cgtaagaata atgtagtttt taacaccatc cctggtatct caactatctc tataaaacag
     4141 catgacgctg tcgtcctctg gctctatccc agatgccgta aaaacgccct gcattgctgg
     4201 cggtatacca gacagtatga cgaatattgc gatgcccaag ataatcctag ataagtcgcg
     4261 tatctattcc catattcgcc aaagcaaaac cacacgaata gcgtaacatg tgcggatgaa
     4321 tctccagtga caacccggca ttaccaccag aagtcgagat aatatggtaa aactgttgcc
     4381 gagaaagcgg attcccctta cgtgataaaa atacccactc gctctcagca tgcggataag
     4441 aagtacggat actcaaccag ttttttaaag cctgaacttc tttgttcaat agcgggtgcg
     4501 ttgttgaaaa gccttttttt aatcgatgga tatatataca ctttgcttta agatcaatat
     4561 ccgaaatcct caatcgacaa atttcactcg cccggaaacc atgaataaaa caaagcaaaa
     4621 tcagacaata attacgtgtt gcatgaggcc cggtatttgc tgctttaagg agtgactcga
     4681 tttcactatg ggtaaggaag ttcctttttt tgttatcagc cttattcctc atcgttttcc
     4741 cttataatta cagacgcgca ctagctgtgc tgggttagca ataatccaac atttttatcc
     4801 tgattatgtt tataaaagcg aacgtttgct taataactta cgtaagtgga ccagttcttc
     4861 tgagcgaact taaatggagt agcgatagtt acttataata actttgccat acaaacgcca
     4921 tgctcttgca tgctatgtac ctttatatat ttatcaatct gatacgaatc taataataac
     4981 cctcatcatt aatgcaacta atcttatcta tatatcatgt gatatgttct gtaacaagta
     5041 aaatcaacat aaaaaattca gcaaaattta atatttgtta aactagccgt ctcaaatatg
     5101 acattattag ttaactgcat ttgccacaaa atggctattt catggaacgc agtaatttct
     5161 tagtggctat tatcatgcta attcttagat ccctagcgat tattcctgcc tggttcgtca
     5221 gcttaggcca tgagattgtt acattccttt ttatcctgga gatatggggg aacgcaggcg
     5281 acgctttttc atctacatcc gttggtaaat atagctttgg tcactacgac ttaagccttc
     5341 ctcaatcaca gctattcgcc gggcgatacg gcaaacaaac taagtgaaac atcatcctga
     5401 aaacaccaac atcaacaagc ctctccagat cgacatcagg agtgaccagt tacaagccac
     5461 aaaccaagaa acatatttca tgatgagaat tatgctcagc ctgatggcga gtaaaccctt
     5521 attttcacca ctctcactga gttagcttgc aaagctttct ttgtttagat tctaaataaa
     5581 ttatttataa aacgtatagt taaaagaaaa tctttagttt ttgaagacca ccgcaagtgt
     5641 tcgtctggct tcacatggca tcttcttctt agaaaaagat cgacatattt tgtgacgcga
     5701 attgcaaatc tggttttgtt gtatggattg cgtgattttt gatctggtat aacaggtata
     5761 aagatgcacc acgatagtca atgagactgg gcatctcgta atctatggca aacatcactt
     5821 cagttctttc acatcgggtg atgaaaatgc acttcagtct gaaaggaata tgaaaatgag
     5881 atcaacagac actctatttt atgactctgg gtaaaatggg ttgagtaagt gatataactt
     5941 acgaatattc aaatcaatta aaaatcagaa gagattttat actcaggtat ttaatctgga
     6001 tctctgttta tttaaataat gttaaaagag aattttcaca ggagacctta tacaaaaaat
     6061 ataaaataca gttacccgtt gccaaagaca ctataagcct ggcaaaaaaa tattacacaa
     6121 cataaatgct aattgtttat gcgggctttg tattgctttc tgtatcctac aaatgagtga
     6181 aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt tcgtccccat
     6241 taatttctca ggctgcgaca ctggacgtac gtggtggata tcgtagtgga agccacgcct
     6301 atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca agcatggaaa
     6361 gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc aatgatgttc
     6421 aggttgaagt taattacgca attaaacttg atgatcaatg gacggtgcgc ccgggaatgt
     6481 taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg tcctgggacg
     6541 cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct taccgacaac
     6601 aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga tatgtcactt
     6661 accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc aaacagaatg
     6721 attatcgcta tgcaaaccac aagaaatggg cgacggaaaa tgcctttgtt ctacaatacc
     6781 atatgacgcc cgatattacg ccatacatag aatatgacta ccttgaccgc cagggtgttt
     6841 acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca tttaaactgt
     6901 agtagacagg agacagtcac aatgaataaa actataatgg cgcttgctat catgatggct
     6961 tcatttgccg caaacgcgtc tgtattaccg gaaactcctg tgccatttaa aagtggtacc
     7021 ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac agcatggtac
     7081 aagctagata cacaggccaa agataaaaaa tggactgcgt tagctgcatt ccctggtgga
     7141 ccgagagagc aagcaacctc tgcatttatt gatggcaatc tgtatgtgtt tggcggcatt
     7201 ggcaaaaaca gcgagggatt gactcaggta tttaatgacg tacacaaata caaccccaaa
     7261 accaatagct gggttaaatt gatgtcgcac gcgccgatgg gcatggcggg tcatgtgact
     7321 tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat cttcaatggc
     7381 tattttgaag atctcaacga ggctggaaaa gattcaaccg ctatagataa aatcaacgcc
     7441 cactattttg acaaaaaagc agaagattat ttcttcaata agtttctgtt gtcttttgat
     7501 ccctcaacac agcaatggag ttacgctggc gaatcgccct ggtacggaac ggctggtgcg
     7561 gcggttgtga ataaaggtga taaaacctgg cttattaatg gcgaagccaa accaggattg
     7621 cgaacggatg ccgtatttga acttgatttc accggtaata atttaaaatg gaataagctt
     7681 gatcccgtct catcaccaga tggcgtcgct ggcggttttg cggggataag caatgattct
     7741 cttatatttg ctggaggggc cggattcaaa ggttcacgag aaaattacca gaacggtaag
     7801 aactatgcgc atgaaggcct gaaaaaatca tatagcactg atattcatct ttggcataac
     7861 gggaaatggg ataaatcggg tgaattatcg caaggtcggg cctacggagt atcattgccc
     7921 tggaataata gtttattaat tattggcggt gaaactgcag gcggcaaagc ggtgacggat
     7981 tcagttttaa tttctgtgaa ggataataaa gtcacagtac aaaactaacg cttcagggcc
     8041 ccggtaaagg ggccctgcta tcaatttgtc atttgtatta cgcgaaaatt atatgaacgc
     8101 aataatatca cccgattatt actatgttct taccgttgct ggtcagtcta atgccatggc
     8161 gtatggcgaa ggactgccat taccggacag ggaagatgcg cctcattcca gaattaaaca
     8221 attagcgaga tttgcgcata cgcatcccgg aggcccgcca tgtcacttta acgacattat
     8281 tccactgact cactgcccac acgatgttca ggatatgcag ggttatcacc atcctctggc
     8341 aacgaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg cacggaaatt
     8401 actgcccttc attcctgata atgcagggat tcttatcgtt ccgtgttgcc gtggcggatc
     8461 ggcttttacc gcgggcagcg aagggacata ttcagaacgg cacggagcca gccatgatgc
     8521 ttgtcgttgg ggaacggata ctccgctgta ccaggattta gtcagcagaa cgcgagccgc
     8581 actggcaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag gcgaatttga
     8641 cttaatgacc agtgactacg cgtcacaccc tcaacacttt aatcatatgg ttgaagcctt
     8701 tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg cgccgtggtt
     8761 ttgcggcgat accacctggt actggaaaga aaatttcccg cattcgtatg aagctattta
     8821 tggcaattat caaaataatg ttttagccaa tattattttc gtcgacttcc agcaacaagg
     8881 tgaaagagga ctgacgaacg cgcctgatga agacccggac gatttaagca cgggatatta
     8941 cggttcagcg taccggtcac cggagaactg gacgacggca ctgcgaagca gtcatttcag
     9001 cacggcagcc cgtcggggga ttatttctga cagatttgta gaagcaattt tgcagttttg
     9061 gcgcgaaagg tgagcgggga gtttattcat gggggatgtc cttgttcgtt ctcagagtag
     9121 acaacaaaaa acaa
//
LOCUS       DAJVQR010000139         9062 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVQR010000139 DAJVQR010000000
VERSION     DAJVQR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            86..742
                     /gene="pdxH"
                     /locus_tag="OQM52_003648"
     CDS             86..742
                     /gene="pdxH"
                     /locus_tag="OQM52_003648"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1565342.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            871..2145
                     /gene="tyrS"
                     /locus_tag="OQM52_003649"
     CDS             871..2145
                     /gene="tyrS"
                     /locus_tag="OQM52_003649"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1565343.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2204..3067
                     /gene="pdxY"
                     /locus_tag="OQM52_003650"
     CDS             2204..3067
                     /gene="pdxY"
                     /locus_tag="OQM52_003650"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1565344.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3111..3716)
                     /gene="gstA"
                     /locus_tag="OQM52_003651"
     CDS             complement(3111..3716)
                     /gene="gstA"
                     /locus_tag="OQM52_003651"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1565345.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3822..5324)
                     /gene="dtpA"
                     /locus_tag="OQM52_003652"
     CDS             complement(3822..5324)
                     /gene="dtpA"
                     /locus_tag="OQM52_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1565346.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(5935..6570)
                     /gene="nth"
                     /locus_tag="OQM52_003653"
     CDS             complement(5935..6570)
                     /gene="nth"
                     /locus_tag="OQM52_003653"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1565347.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6570..7265)
                     /gene="rsxE"
                     /locus_tag="OQM52_003654"
     CDS             complement(6570..7265)
                     /gene="rsxE"
                     /locus_tag="OQM52_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1565348.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7269..7889)
                     /gene="rsxG"
                     /locus_tag="OQM52_003655"
     CDS             complement(7269..7889)
                     /gene="rsxG"
                     /locus_tag="OQM52_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1565349.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7893..8951)
                     /gene="rsxD"
                     /locus_tag="OQM52_003656"
     CDS             complement(7893..8951)
                     /gene="rsxD"
                     /locus_tag="OQM52_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1565350.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2320 a         2272 c         2301 g         2169 t
ORIGIN      
        1 tgtcagttac aaaatccaca gcgttgagat ttttccaggg gcggcgcaca atagcgtcac
       61 ccactgacaa tccgtaaaga aaaccatgtc tgataacgac gaattgcagc aaatcgcgca
      121 tctgcgccgt gaatacacca aaggcgggtt acgccgccgc gatcttcccg ccgatccatt
      181 aacccttttt gaacgttggc tctctcaggc ttgtgaagcc aaactggcgg accctaccgc
      241 gatggtggtc gctaccgtgg atgaacatgg tcagccttat cagcgcatcg ttttactcaa
      301 acattacgac gaaaaaggca tggtgtttta caccaacctc ggtagccgta aagcacatca
      361 aattgaaaat aatccgcgcg ttagcctgct gttcccgtgg catacccttg aacgccaggt
      421 gatggtgatc ggtaaagcag aacgactttc gactctcgaa gtgatgaaat attttcatag
      481 ccgcccgcgt gatagccaga ttggtgcatg ggtttcgaag cagtccagtc gcatttctgc
      541 ccgcggtatc cttgaaagta aattcctgga gctgaagcag aagtttcaac agggcgaagt
      601 gccattgccg agcttttggg gcggttttcg cgtcagcctt gaacagattg agttctggca
      661 gggcggtgag catcgcctgc atgaccgctt tttgtaccag cgtgaaaatg atgcgtggaa
      721 gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg ctttaatcgc tggtactcct
      781 gattctggca ctttattcta tgtctctttc gcatctggcg aaaagtcgtg taccggcaaa
      841 ggtgcagtcg ttatatacat ggagattttg atggcaagca gtaacttgat taaacaattg
      901 caagagcggg ggctggtagc ccaggtgacg gacgaggaag cgttagcaga gcgactggcg
      961 caaggcccga tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt gcatttgggg
     1021 catcttgttc cattgttatg cctgaaacgc ttccagcagg cgggccacaa gccggttgcg
     1081 ctggtaggcg gcgcgacggg tctgattggc gacccgagct tcaaagctgc cgagcgtaag
     1141 ctgaacaccg aagaaactgt tcaggagtgg gtggacaaaa tccgtaagca ggttgccccg
     1201 ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg cgaacaacta tgactggttc
     1261 ggcaatatga atgtgctgac cttcctgcgc gatattggca aacacttctc cgttaaccag
     1321 atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg aagatcaggg gatttcgttc
     1381 actgagtttt cctacaacct gttgcagggt tatgacttcg cctgtctgaa caaacagtac
     1441 gatgtggtgc tgcaaattgg tggttctgac cagtggggta acatcacttc tggtatcgac
     1501 ctgacccgtc gtctgcatca gaatcaggtg tttggcctga ccgttccgct gatcaccaaa
     1561 gcagacggca ccaaatttgg taaaactgaa ggcggcgcag tctggttgga tccgaagaaa
     1621 accagcccgt acaaattcta ccagttctgg atcaacactg cggatgccga cgtttaccgc
     1681 ttcctgaagt tcttcacctt tatgagcatt gaagagatca acgccctaga agaagaagat
     1741 aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg cggagcaggt gactcgtctg
     1801 gttcacggtg aagaaggttt acaggcggca aaacgtatta ccgaatgcct gttcagcggt
     1861 tctttgagtg cgctgagtga agcggacttc gaacagctgg cgcaggacgg cgtaccgatg
     1921 gttgagatgg aaaagggcgc agacctgatg caggcgctgg tcgattctga actgcaacct
     1981 tcccgtggtc aggcgcgtaa aactatcgcc tccaatgcca tcaccattaa cggtgaaaaa
     2041 cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc tgtttggtcg ttttacctta
     2101 ctgcgtcgcg gcaaaaagaa ttactgtctg atttgctgga aataatgcat taagtggaaa
     2161 gggggagtga gaaatcactc cccctggttt ttatacaggg aacatgatga aaaatattct
     2221 cgctatccag tctcacgttg tttatggtca tgcgggtaac agtgcggcag agtttccgat
     2281 gcgccgcctg ggcgcgaacg tctggccgct gaacaccgtt caattttcta atcacaccca
     2341 atacggcaaa tggactggct gcgtgatgcc gcccagccat ttaaccgaaa ttgtgcaagg
     2401 cattgccgcc attgataaat tacacacctg tgatgccgta ttaagtggct atctgggatc
     2461 ggcggagcag ggtgaacata tcctcggtat cgtccgtcag gtgaaagcag cgaatccgca
     2521 ggcgaaatat ttttgcgatc cggtaatggg tcatccggaa aaaggctgta tcgttgcacc
     2581 gggtgtcgca gagtttcatg tgcggcacgg tttgcctgcc agcgatatca ttgcgccgaa
     2641 tctggttgag ctggaaatac tctgtgagca tgcggtaaat aacgtcgaag aagcggttct
     2701 ggcagcgcgc gaactcattg agcaagggcc acaaattgtg ttggttaaac acctggcgcg
     2761 agctggctac agccgtgacc gttttgaaat gctgctggtc accgccgatg aagtctggca
     2821 tatcagccgt ccactggtgg attttggtat gcgccagccg gtaggtgttg gtgatgtgac
     2881 gagcggttta ctgctggtga aactgcttca gggggcaacg ctgcaggagg ccctggaaca
     2941 tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa gcaatgcagg aatatgagct
     3001 gcaagtggtg gctgctcagg atcgtattgc caaaccagaa cattacttca gcgcaacaaa
     3061 gctctgaaat atctcatttc ggcccacaac gacctgtggg ccgatggcta ttactttaag
     3121 ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg cataaatgcc
     3181 gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt atgcccagcg cagaaccgta
     3241 aacagatagg catcagcaat tgtaaatctt tgcccgcaga tccagtgctc atccttcagt
     3301 gcctcgttca catattgcag cttcttatcc agctgcgcgc gaactgtcgg tttgtactct
     3361 tccggtgtat ctgggcgaaa cagaggtgtg aaacctttat gcagctcggt ggcgatgtaa
     3421 ttcagccatt cgatggtttt atagcgggaa atactgttta ccggtgccag caactggcgg
     3481 tcggggacgc tgtcggcaag atactgcata atcgcaacgc cttccgtcag caaagtacca
     3541 tcgtccagca gcaatgcagg cacctgcccc ttagggttaa cggaaaagta atcgtcaccg
     3601 ttttcgagac gttttttcat taaatccaca ctgacgagcg taaaatcctt tccgctctca
     3661 cgcagggtga tatgggaagc gagagagcag gcgcccggtt tgtagaacaa tttcatcggt
     3721 aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaaagccgc
     3781 taacgaaaag ttagcggctt gtaaaagagt ttccctgaat tttacgctac agtagctttc
     3841 gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca gtttaggcgc ggtcagcagc
     3901 atcagtactg caataacggc agtagcgaca ccaatctgca agaatacgcg accatagact
     3961 tccagtgaca tcagcgggtc ggtaacgtta tccggcacag ccatcatacc cgcaacataa
     4021 ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc tacccataat gaagcccatc
     4081 agacgctgcg gaacgagttg tgcaaccatt gccagaccca gaccagagat catcagttcc
     4141 ccgatgctct gcaggccata gcttgcgacc agccagctga cagacacgat acctgcgtca
     4201 gacgcgaatt tcgcacccag cggcagaatc aggaacgcac cagaacacat caccatgccg
     4261 attgcaaact tggttggcat cggcagggta tcacccatct tgttatagat agcggccaga
     4321 atcggactac cgatgatgat ccagaacggg ttcagtgcct gatactgctc aggttctacg
     4381 gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa agaagttcag tgacgttggc
     4441 atctggctgt acagcacgaa gaagatgatt gcttcgagca tcaggatgaa ggcaacgatc
     4501 attttacgac gcgcagcacc tttcatcgcg aaagcttctt taccgaagat aaccacaata
     4561 ccgaaggcaa caacgcccag cgccatacgc gcaacttcct gattgtgcag tagccaggtg
     4621 gcaacagcga tcagcgccac aacaccaata atggtcagca acaggttacg gtagttgact
     4681 ggttcaaagt ctggttttga accgtactgt ttaacccagc gttggcagaa ggcgaagtta
     4741 acgatagtga tcagcaggcc tacaacgctc aacgcaaacg caacactcca gccgtatttc
     4801 gcggccagcc acggggtggc tatcatagag aagaaagagc cgatgttgac ggacatgtag
     4861 tacatggtga atgcaccgtc cagacgtggg tcgtttttct catagcatgt agaaagcaga
     4921 gaagacgggt tagctttaaa caggccgtta ccgaccgcaa tagccgccat acccatataa
     4981 acaataccgg cgtcgtgacc agaccaggca accagagcat aaccaatcgc cagcacaata
     5041 gcgccgagca taattacgcg tttagtaccc agtaccttgt cacctaacca gccgccgata
     5101 gcgaccagac cataaaccag ggcactaaag gaagagaaaa gggtgattga atccgcttca
     5161 gacataccca gttgtttaac caggtaaaca gccataattc cttgtaggcc gtaataacca
     5221 aaacgttccc ataactcaat cgagaagatg agatagaacg ccttcggttg tttgaaagcg
     5281 ttcaaactga cgctttcagt tggtttttgg tttgcagtgg acacttttac ctcttttttt
     5341 acatcccata ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg
     5401 ttatagtggg aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct
     5461 ttgtaataat ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat
     5521 ccagcgttaa atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga
     5581 tgatggcttg gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt
     5641 ttctgaaaaa tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta
     5701 aatcatttta acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc
     5761 acgaatgaat agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg
     5821 caaacggatt acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt
     5881 taaaagagga taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat
     5941 gtcaactttc tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt
     6001 gcgggcaatg caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa
     6061 ctctgccgga accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc
     6121 aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc
     6181 gaatgcagtg tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag
     6241 cgcagcacga tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt
     6301 gatgatattt tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc
     6361 ttcaacgcct agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt
     6421 cgccttatta acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc
     6481 aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt
     6541 gaggatctcc agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat
     6601 gcacgttcgg cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt
     6661 cctgccagca tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa
     6721 ggggagtcgg tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca
     6781 tctgcaccgt caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac
     6841 atggcgcagg ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct
     6901 tttttggcgg cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga
     6961 ataaaaatcc ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca
     7021 gcgctgacca ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc
     7081 cagtgacgca gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt
     7141 cctaaaccca gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc
     7201 agttggacca gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg
     7261 ctcacgcttt attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg
     7321 tacaatccgg cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg
     7381 aactggtcga aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg
     7441 attttttttc ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca
     7501 agccctggcg tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg
     7561 gctcccacca gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg
     7621 gcggctaccg gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac
     7681 tctggcgcag ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc
     7741 agcacctgat caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg
     7801 gtcatctggt tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta
     7861 atgccgtgtt ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc
     7921 ggacgcgtgt agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc
     7981 acgccgtcag gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg
     8041 ccgaacatca gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag
     8101 aatgcgccga gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct
     8161 ggtgagaaca accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga
     8221 atatgccagc gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga
     8281 tttacccatt gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc
     8341 tgttcaaccg aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga
     8401 ctaatgccat caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg
     8461 ctgaaaataa cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc
     8521 ggtaaccagc tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt
     8581 gccggattaa acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca
     8641 aacaccgtac ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc
     8701 aataagcctg tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt
     8761 agtttgagta cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg
     8821 agagtacccc aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac
     8881 accaacagca taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt
     8941 ctgaatacca tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg
     9001 cgggcaatag ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca
     9061 tt
//
LOCUS       DAJVQR010000140         9012 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVQR010000140 DAJVQR010000000
VERSION     DAJVQR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OQM52_003657"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OQM52_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1565351.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OQM52_003658"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OQM52_003658"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1565352.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OQM52_003659"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OQM52_003659"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1565353.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OQM52_003660"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OQM52_003660"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1565354.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="OQM52_003661"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="OQM52_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1565355.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="OQM52_003662"
     CDS             5545..6018
                     /locus_tag="OQM52_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1565356.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="OQM52_003663"
     CDS             6047..6589
                     /locus_tag="OQM52_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCS1565357.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="OQM52_003664"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="OQM52_003664"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1565358.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="OQM52_003665"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="OQM52_003665"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1565359.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="OQM52_003666"
     CDS             7986..8243
                     /locus_tag="OQM52_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1565360.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="OQM52_003667"
     CDS             complement(8219..8773)
                     /locus_tag="OQM52_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1565361.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2254 a         2188 c         2249 g         2321 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgatata gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaga agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag ag
//
LOCUS       DAJVQR010000141         8764 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVQR010000141 DAJVQR010000000
VERSION     DAJVQR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            7..438
                     /locus_tag="OQM52_003668"
     CDS             7..438
                     /locus_tag="OQM52_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1565362.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OQM52_003669"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OQM52_003669"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1565363.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OQM52_003670"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OQM52_003670"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1565364.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OQM52_003671"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OQM52_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1565365.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="OQM52_003672"
     CDS             complement(4440..5147)
                     /locus_tag="OQM52_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1565366.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OQM52_003673"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OQM52_003673"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1565367.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="OQM52_003674"
     CDS             complement(6005..6208)
                     /locus_tag="OQM52_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1565368.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6397..7875
                     /locus_tag="OQM52_003675"
                     /pseudo
     CDS             6397..7875
                     /locus_tag="OQM52_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7872..>8764
                     /locus_tag="OQM52_003676"
     CDS             7872..>8764
                     /locus_tag="OQM52_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1565369.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         2035 a         2389 c         2251 g         2089 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg
     8401 atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca
     8461 gaaattcata ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg
     8521 ttcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc
     8581 tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag
     8641 cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta
     8701 aacagctcac gcccgaccgt cagtggagca agtggataga cctaccaggt attgaacctg
     8761 aaca
//
LOCUS       DAJVQR010000142         8652 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVQR010000142 DAJVQR010000000
VERSION     DAJVQR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OQM52_003677"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OQM52_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1565370.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3190)
                     /gene="hemX"
                     /locus_tag="OQM52_003678"
     CDS             complement(1967..3190)
                     /gene="hemX"
                     /locus_tag="OQM52_003678"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1565371.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(3212..3952)
                     /gene="hemD"
                     /locus_tag="OQM52_003679"
     CDS             complement(3212..3952)
                     /gene="hemD"
                     /locus_tag="OQM52_003679"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1565372.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3949..4890)
                     /gene="hemC"
                     /locus_tag="OQM52_003680"
     CDS             complement(3949..4890)
                     /gene="hemC"
                     /locus_tag="OQM52_003680"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1565373.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5277..7823
                     /gene="cyaA"
                     /locus_tag="OQM52_003681"
     CDS             5277..7823
                     /gene="cyaA"
                     /locus_tag="OQM52_003681"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1565374.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7863..8183)
                     /gene="cyaY"
                     /locus_tag="OQM52_003682"
     CDS             complement(7863..8183)
                     /gene="cyaY"
                     /locus_tag="OQM52_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1565375.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8231..>8652)
                     /locus_tag="OQM52_003683"
     CDS             complement(8231..>8652)
                     /locus_tag="OQM52_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1565376.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1968 a         2236 c         2229 g         2219 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtgcagg tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca
     2101 ggttacgcac gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg
     2161 gaagatccat cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg
     2221 tggtggcatc atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct
     2281 ggcgataagt ctcttcctga tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc
     2341 gaatattttc gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat
     2401 cacgacggcg aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga
     2461 tacgccattc gctgatggaa ctggaaagct cttcaccgtc tgaatccatc ggcgaaccat
     2521 cgctgtcatt atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa
     2581 ggatgatgcc gtcataatcc acctgcgata ctgcagaaag gctggcgata tcatcggtaa
     2641 ttgcccgacg aacggtaatc agactcgggt cattcatatc cgccaggctg gcgtctgcac
     2701 ttttcagcaa cgctgcagag gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga
     2761 gtttcaccag aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg
     2821 tggcgatctt ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg
     2881 cctgcttaag ctgcgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct
     2941 cctgggcttt ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat
     3001 tgacggcctg ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga
     3061 tagccaccgc gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag
     3121 gttgtgacgt ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt
     3181 gttccgtcat tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt
     3241 tgtcagcgtt atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca
     3301 aacgctcact gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg
     3361 ggatcagcga ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct
     3421 cgcgggattg ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat
     3481 aacattcaca aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac
     3541 gaccgccatt gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta
     3601 attgtagcaa gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta
     3661 cggtatgtag cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc
     3721 atttacgatc ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg
     3781 caaacaacag atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc
     3841 gacccggaga aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac
     3901 ggctcactaa ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc
     3961 ctctccgtta tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc
     4021 cagcgaaatc cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat
     4081 ctgcgaaccg tccggcgcgc cgaccagcgc acatagccag atttcgccat caataagctc
     4141 ggcgtagcta ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc
     4201 tgcggtaacg cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg
     4261 tgtatcatca aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat
     4321 ctcgggtggt aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac
     4381 ggcaagaatg atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt
     4441 gccgcgcagg gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg
     4501 taaactggac gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt
     4561 ggacacaaag gcatcgcgag gatcttcacg ctcgcaaata gtgaccagcc ccagaccttg
     4621 cgggaattca accggcacat ctttcattga atgtacggcg atatcagcgc gattctccag
     4681 gagcgcgact tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt
     4741 atcaagaatc acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg
     4801 gctcgccatc aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg
     4861 tgtggcaatt cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc
     4921 atcctaacat ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa
     4981 accgtaaaat gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga
     5041 atttacagag aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc
     5101 agcaaggtgt taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc
     5161 aggcgcgaaa agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt
     5221 accgttgatg ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt
     5281 acctctatat tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc
     5341 gcgcgcttgc tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt
     5401 tgcactatca ccatccgcga atgccgggtt accttgatgg taacgttccc aaaggcattt
     5461 gcctttacac gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg
     5521 gaatgtcagt acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg
     5581 gcagcacctc gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc
     5641 aatcctggct cgatagcgaa gagcgccagt tactacaacg taaatgcagc ctgctggaaa
     5701 gctgggccgc ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc
     5761 gtcataatga aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc
     5821 tgcttgacga attttatcgt actgccgtgc gtctcgccgg taagcgtatt ctgtggaata
     5881 tggtgccgtg cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg
     5941 gcgtgctgac gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag
     6001 agtactttgg tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg
     6061 tgctgaaaac actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg
     6121 cgaaagatat caaacagcgt ttgcacgacg gcgagattgt atcgtttggc ctcgatccat
     6181 actgcatgat gctggagcgt gttactgaat acctgacggc gattgaagat ttcacccgtc
     6241 tggatttagt acgtcgctgc ttctatttaa aagtgtgcga aaaactcagc cgtgaacgcg
     6301 cctgtgtagg ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg
     6361 aagctcgtct ggcaatgctc gataaccgtg ctaactggaa gattgatcag gtgcgtgagg
     6421 cgcacaacga gctgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc
     6481 gtcgcaataa ccttagcgtc tccgccagtc cgcaggatat cggcgtactg acgcgtaaac
     6541 tgtatgccgc gtttgaagca ttgccaggta aagtgacgct ggtaaacccg cagatttcac
     6601 ccgatctctc ggaaccgaat ctgaccttta tttatgtgcc gccgggccgg gctaaccgtt
     6661 caggttggta tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc
     6721 tggaatataa ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga
     6781 cctcgcgcac ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg
     6841 agatggtcgc cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg
     6901 cgctctacag cccgtgtgag attcgccatc tggcgattat cgttaacctg gaatatgacc
     6961 cgacagctgc gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca
     7021 gctttggcga gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt
     7081 ggaacgaagt gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa
     7141 ctattctcgg caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt
     7201 atagccagca tctgcgcggc ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta
     7261 ttgaactgcg cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt
     7321 ctggtcaaac ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga
     7381 acgccatcga gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg
     7441 ttgaaaccaa tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca
     7501 tccagttctt tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg
     7561 aaagcaaccg ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg
     7621 acgtcagtcg cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca
     7681 acttcaacct gccgcagttc tatcagattg tgaaggttga tggtcgcgaa caggtgattc
     7741 cgttccgaac aaaatcgatt ggcaacctgc cgcccgccaa tcaggatcac gatacgccgc
     7801 tattacagca gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa
     7861 tcttagcgga aactgactgt ttcacccgcc tgttgcgtcg ccgcctgttc cagcaaatcc
     7921 cagaaggttt cgccgctgcg atcgcaaatc cattcatcgc ctttcaggtc aaaatggtag
     7981 ccgccctgtt tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt
     8041 ttgctgccgt tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg
     8101 ctgtcgccat ccctgtcgtc cagacgctct tcaatggtca gccacagttg atcagccagg
     8161 cgatgaaatt cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag
     8221 cggcatgggg tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat
     8281 ccgtcaccgg aataatgatg tctctgtttt gaacttcacg tacgttctcc tctctgacac
     8341 gagttttcgc cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa
     8401 cattgcgatc gtcataagcg aagcgatcgt ttttagttag cgaaccgtta actggaataa
     8461 tttgcaagct ctttgaccgt aattggcgga taaagtaagc atgctgtttg ccgctattaa
     8521 atgagcgatc aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac
     8581 caaatgtcga taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa
     8641 tcagatggaa tt
//
LOCUS       DAJVQR010000143         8510 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVQR010000143 DAJVQR010000000
VERSION     DAJVQR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(81..272)
                     /locus_tag="OQM52_003684"
                     /pseudo
     CDS             complement(81..272)
                     /locus_tag="OQM52_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            270..594
                     /locus_tag="OQM52_003685"
                     /pseudo
     CDS             270..594
                     /locus_tag="OQM52_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(624..3536)
                     /locus_tag="OQM52_003686"
     CDS             complement(624..3536)
                     /locus_tag="OQM52_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565377.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3539..3655
                     /locus_tag="OQM52_003687"
     CDS             3539..3655
                     /locus_tag="OQM52_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1565378.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4019..4861
                     /locus_tag="OQM52_003688"
     CDS             4019..4861
                     /locus_tag="OQM52_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1565379.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4864..5952
                     /gene="capU"
                     /locus_tag="OQM52_003689"
     CDS             4864..5952
                     /gene="capU"
                     /locus_tag="OQM52_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1565380.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5957..6907
                     /gene="virK"
                     /locus_tag="OQM52_003690"
     CDS             5957..6907
                     /gene="virK"
                     /locus_tag="OQM52_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCS1565381.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6972..7916
                     /gene="lpxM"
                     /locus_tag="OQM52_003691"
                     /gene_synonym="msbB"
     CDS             6972..7916
                     /gene="lpxM"
                     /locus_tag="OQM52_003691"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1565382.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(8070..>8510)
                     /locus_tag="OQM52_003692"
     CDS             complement(8070..>8510)
                     /locus_tag="OQM52_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCS1565383.1"
                     /translation="RGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFIS
                     LLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERL
                     WQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT         2256 a         1930 c         2028 g         2296 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta aggccgccgg ggacgttttt ttaccaccgg gagtttgggc
      121 ataacggtgc tttcagtaag tacgacaggt actgtggagg cacttccgta aagaagggca
      181 tcaagctcct gctccgacat acctgcaggc aaaggccatg aaagccctga tttccggaag
      241 cgagcgaaca gtccacaaac tgttgaacgt ggtgtcagcg gcggacaggg ataagccctc
      301 gtcagcagtt ccgtcagcac tgcgacagcg ttctttaccc ggtcaaaaca gcgttgcggt
      361 gcaccacgcc tgacggatga actgcgtgct cagggttacc actttaacgt aaaaaccgtg
      421 gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt cagcccggtc
      481 agttaccgcg cacatggcct gcccgtgtca gaaaatctgc tggagcagga tttttacgcc
      541 agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga tgaagttcgg
      601 ttacatcctg tcagcacgga acctcacgct ttttgagcgg cacgacggat cccccccacc
      661 cagtatccga cagacttttc actgcgttcg taaaaaccct tcttccctcc cagcatatcc
      721 actaattccg gtgacatatc ctgcagataa gtctgcaaat catgccccag atcttccggt
      781 gactgcaact cataaaccgc gcgccacaca tcacccttcc ttattttaag ccccgggtta
      841 agaatatact gtccgtggtt cagcctcaga aaaagctttt ttccataagg atttttgctg
      901 ttaacttcat gttgagagag ctggctgctg atataactcc ttcgtttgcg gcgttccggc
      961 agcaacgctt caggaagatg aaccacacac tctgccagtc gtgccgcact gaaggcttcc
     1021 gaaaaaaaca gagcacttcc cagatgcgtg tggaacagcg cctgcatcag gatcaccaga
     1081 aacattgcca tctgccggtt gtccagtttg atgaggcgtc cttccacctg cagtgacaga
     1141 ctttcaggtt tcagcagggt atacaactgc gcacttttct gttgcgcata acggggcgta
     1201 ctgaccgccg ctgacagaac ttgatgataa gccgccagcc cgtcattacc ggtcagcaag
     1261 ggatctgcac cacgctccag cagtaactga accagtgcaa tattacctgc ccgggctgct
     1321 gacatcagcg gagtaagatt aaacggcgaa cggtgatcaa caccataacg ttcaatatcc
     1381 cgcaacacgg caaccgggtt attcattgag tatggccgga aatacttcag ctccagtaac
     1441 tgcagagtct tcgttctggg ctgccttgtg ggcttaaaat cttcccggta cagtgcatta
     1501 tacagcggga acagggaata aatcagactg tactccagcg cctgcagttg tatggtttta
     1561 tcggctgtac ctgcgaggat atgctgtttc cactgtcgtg catcctctgc ggtgataatc
     1621 tgccatggca tctcagatgt ctgcaggatc cggctacgga tggtgtccgc ctgctcctgc
     1681 ttcccctgtt tttcaagcag atgtgcggtt ttctgccact catcacggct ggaaatctct
     1741 tcctgcagat tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca cagaggatgc
     1801 tccacctgct gttcaacaag atacacattg tgtttagcac gggtgagcgc aacatacagc
     1861 gcgttggtaa aaaatttata gatttccgct gaacgatcct gcctgtcccg cggacgggaa
     1921 taacgggcct cgccctcaag atcagccggt gtaagaccct cgcaaatatc atcaaatgcc
     1981 tgccgggcag cgcttactat attgtaaagg atcaccgttt cgtattccag ccccttaacc
     2041 tcctgaaccg aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc tttctgttcc
     2101 ggatgcatca caatgaccgc tacacgattc gaaagtgagg ttttggcatt cagttcctgc
     2161 tttgtctctt ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc gcagcttctg
     2221 acaaaatggt gactttcccg atcaatagca ctaaagcgga cctgtttgag tctcagcaac
     2281 cggttagcca gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag gatattaacg
     2341 gttgtgtcgt ttccctgttg ctgccggaaa aagagacttt tgagcgaact ccatgagaaa
     2401 aagctcggat gaactatctg attggcatca ccacagagta aaaactgacc gggatgacgt
     2461 agcgttttca gtaccagttg cagttgaaca ggcgtcatat cctgaacttc atcaacaaaa
     2521 atgacatcat aacggggagt tgccaacgaa agatactgat gactgagcag attactgtca
     2581 tactgatgcg actgtttcag ccatgcgata taacgttcaa acaggacata gaccgtcggg
     2641 cggtcctcca tgccatagag cgactgccgt atccccagtg acagatacgc ctcccgggac
     2701 agcggcccat tcccggaggc aaccgcaccg atcacgcccc tgaactcctc atacagggta
     2761 tgggcagccc ccagtgcggc gatagccctg ttgcggggca gccagtcgct gaacgcactg
     2821 aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt cagcgaaaga
     2881 aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt acgcgctttt
     2941 tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg cttcattttt
     3001 tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag cggtagcggg
     3061 tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag gcggtggaaa
     3121 cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac aggatccggc
     3181 actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag aaaacgtgaa
     3241 gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc accgtggtag
     3301 cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta aagattggtg
     3361 tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg tgcaaatttt
     3421 ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt catggctgcc
     3481 ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact gaacaacctt
     3541 gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc agttatggaa
     3601 taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc agtagctgca
     3661 ggcctgtcat gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg tcaccccgta
     3721 aataaactct gcgctgaaat tccttttttt cattggagta cctgtgttgt tctgaggtga
     3781 acatatcacc tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt cttagtgagt
     3841 cgtttttaaa agatatgata atatatcatt gtttatgaat tgttgatgtt taatttagta
     3901 aattatatca aatatgccat gggtttgcta tgttattcat ccagtatctg gaaataaaat
     3961 gcaccagctt tattgggttt tccttttgtc tctgcttgct aggtgccaga aactttaaat
     4021 gttaaacgaa gggggcattt tgttcaaagc caatcatgtt ccggtattaa tgtatcatca
     4081 tgtcagtcat tgccccggac tggttacact ttctcccgtg acattccgga agcaaataaa
     4141 atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt ttttttatcg
     4201 gggcggaaaa ctcccccgga aaagcgttat gttgacattt gatgatggat accttgacaa
     4261 ctggtttcag gtatatcctt tattaaaaga gtttaatctc aaagcacata tttttttaat
     4321 taccggtttt attgggaatg gtccggttcg tcactctcct ggaaaggaat attctcatcg
     4381 cgattgcgaa catcaaatag cgacagggaa tgctgataat gttatgcttc gctggtctga
     4441 agtaaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacgc atactcatac
     4501 acgctgggat aaaaagttca gttcacgaga agaacagtgt aaacatcttc ggcaggactt
     4561 gttgagcggg agggaatatt tgaaagaaat gacaggaaaa tgcagtaaac atttgtgttg
     4621 gccggaaggt tattataaca aggattatat tcaggtcgct gaagaacttg gattctatta
     4681 tttatataca acagaaagaa gaatgaatgc tcctgctaaa ggaactacac ggataggaag
     4741 aataagcacc aaagaacggg aaagttgcgc atggctgaaa agacgacttt tttattatac
     4801 aaccccgttt ttttcatcgt tgctggcctt tcataagggg ccaagattac cggatgatta
     4861 ataatgaata tactatttac ggaatcatca ccgaatatag gtggtcagga attacaggct
     4921 gttgctcaaa tgaaggccct gaagaaaatg gggcattcag ttctgcttgt ctgcagggaa
     4981 aacagcaaaa ttgcttttga agccagtaaa ttggggattg atatcacatt cgcgttattt
     5041 cgaaacagtc ttcacatccc tactgcatgg agattactcg gaatagttca tggttttcag
     5101 cccaatgcaa tcgtttgtca cagtgggcat gatagcaata ttgttggttt agtacggtta
     5161 ttcactagga aacatccatt cagaattatc aggcaaaaga catatttgac acgaaaaaca
     5221 aaagtttttt caataaatca tttttgcgat gaggtgattg ttcccggaac aagtatgaag
     5281 acacatctgg agcaggaagg atgtcgaacc cgggttactg ttgtgcctcc aggctttgat
     5341 ttccagaaat tatacgttga ttcccgaaac agtttgcctc caaatgttct ttcttggctg
     5401 gcgtcccgaa ggggatgccc cgttattgct caggtaggaa tgttgcgccc ggaaaaaggg
     5461 cacgaattta tgttgaattt actgttccat ttaaaaatga atggacgaca gttctgttgg
     5521 ttgattgtgg ggtctggttc gcctgaactg cgggagcatt tacagtatca gattgacagt
     5581 atgggcatgc atgatgatgt ttttattgct gacaatgttt ttcctgccgc ccccgtatat
     5641 cgggttgcca gtctggtggt tctgccttca gaaaacgaat cgtttggtat ggtactggca
     5701 gaagcatcgg cattttctgt gcctgtactg gccagtcaga ttggtggaat ccctgatgtt
     5761 attcagaata accagaccgg gacattgtta ccagcaggta ataagcacgc atggatgtgc
     5821 gccctgaatg atttttttaa tgaccctggg cgtttttatc agatggctcg ccaggcaaaa
     5881 caggatatag aagagcggtt tgatattaat aaaactgcgt taaaaatact cacattagcg
     5941 aagcacaagt aacaatatgt tttctgtaag taacttatca tttatcggtt tccttaaaag
     6001 gattgttttt tcctcagatt cactcccggg gaagtgggaa cacagaaaat tccggttcat
     6061 gtacattttg cgatgtgcta taaatccggt tgccagtatt cgatattatt acgaactgcg
     6121 ttccttgcag tgcattgagg atattctggc aatacaaccc acgttgccag caagaattca
     6181 tcgaccttat ttgcataaag gggggcgagc ctggtcccgg gggcaatata ttcttgagca
     6241 ttatcgcttt gtgcaaaatt tgccagaaaa atattctgaa tttttgtttc cacaaaaatc
     6301 ggtatcatta gtccaattca tcgggaaaga tggagaggat tttgatatcc agtgttctcc
     6361 cagtggtttt gaccgggaag gagagttgat gttgtcattg tttttcaata aaatagtgat
     6421 tgcccggtta acattttcag tcattctgac tcaaaatgga cacaccgctt ttattggcgg
     6481 attacaggga gcccccaaaa ataccggacc tgatattatt cggtgcgcaa cccgggcctg
     6541 ttatgggctt ttcccaaaac ggattatttt tgaagcattt tgtgcgttga tgaaagcttg
     6601 taatgtctct gagtgtctgg cagtcagtga gcacagtcat gttttccggc aattgagata
     6661 ctggtatcag aaacgcaaaa cctttgttgc tgtctacagt gatttctggg agtctgtagc
     6721 agggaaaacc tgtggtgact ggtacaaatt accaacacag gtggttcgaa agccgctaag
     6781 caatatagct agtaaaaaac gctctgagta tcgaaaacga tacgctttgc tggattatat
     6841 ccatgaaact gctatccgct ctttggatgc atatcctgta aactcagaac atcaggactt
     6901 aaattaaggt ttgatgtatt ctctgaataa aatattaatg atgattttgg taggggcatt
     6961 cgcactaaat aatgaaaaaa tacaaatccg agtttattcc tgaatttaag aaaaattatc
     7021 tttcccctgt ttactggttt acatggttcg ttttgggaat gattgcaggt atttcaatgt
     7081 ttcccccttc attcagagat cctgtcttgg ccaaaatagg gcgttgggtg ggcagattga
     7141 gcagaaaagc tcgtcgcagg gcgacgatta atttatcgct ttgtttcccg gaaaagagtg
     7201 atacagaacg ggaaataatt gtcgacaata tgtttgccac agcattgcaa tccatagtga
     7261 tgatggcaga actggcaatt cgtggtccgg aaaagttcca gaaacgagtg ttctggaaag
     7321 gcctcgaaat tcttgaggaa attcggcaca ataacagaaa tgtgattttt ctggttcccc
     7381 atggctggag cgtggatatt cctgcaatgt tgctggcagc tcagggggaa aaaatggccg
     7441 ccatgtttca tcagcaacga aatccagtga ttgattatgt ctggaattct gtacggcgta
     7501 aattcggggg gcgcttacat tcccgggagg atgggataaa accatttatt cagtcagtac
     7561 gccagggata ctgggggtat taccttccag atcaggatca tggtcctgaa tacagtgaat
     7621 ttgctgattt ttttgcgacc tataaagcga cattaccaat tattggacgt ctgatgaaca
     7681 tcagtcaggc tatgattata ccgcttttcc cggtttatga tgaaaaaaaa catttcctga
     7741 ctattgaagt tcggccacca atggatgcat gcattgccag cgcggacaat aaaatgattg
     7801 cccgacaaat gaacaaaaca gtggaaattt tggtggggtc acatccggaa cagtatatct
     7861 gggttttaaa attgttaaaa acgcgcaaat caaacgaagc ggacccgtac ccttgaaatt
     7921 ataaagtaca ggtatttcca ctagttgttt cttacaggtt accaatcgaa acacatcccc
     7981 cttccgtgtg ttgcccgttt ggcacggagg actgtgtaga tgcagttcgt taaatttaat
     8041 ttatgcctaa atagctgcgc ttaataccgc tacacttttg ccagcccatg tttgcctccg
     8101 gggaatgggc tgacggtttc cataaaatgg cgaacttttt tcaacagttg ccacattgag
     8161 cggcactgat gattacgcgt tattgtgtcg tgaagtgcct gccatagccg ttcaacatga
     8221 ttcacccatg gcgagtaaac cggctgataa atgcccctga acttcgggtt ctccttcagc
     8281 cagctctgtg tttcccggct tttgtggata atgtagttgt ccacgatcag cgtgatggtt
     8341 ttcgcacgac ggtatgtcgc tttaagccgc ttcagcaggc tgatgaacag cgccgaactt
     8401 ttgctgttgc cgcccacata gctgacttta cctgtcccgc tgtgcagcgc tccggccaga
     8461 taatattttt cattctgtcc cggcgtgacc acccgttttt gctgtccgcg
//
LOCUS       DAJVQR010000144         8363 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVQR010000144 DAJVQR010000000
VERSION     DAJVQR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="OQM52_003693"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="OQM52_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="OQM52_003694"
     CDS             1128..1235
                     /locus_tag="OQM52_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565384.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="OQM52_003695"
     CDS             complement(1288..2046)
                     /locus_tag="OQM52_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1565385.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="OQM52_003696"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="OQM52_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1565386.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="OQM52_003697"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="OQM52_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1565387.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDVFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="OQM52_003698"
     CDS             3759..4619
                     /locus_tag="OQM52_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1565388.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="OQM52_003699"
     CDS             complement(4660..5196)
                     /locus_tag="OQM52_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1565389.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="OQM52_003700"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="OQM52_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1565390.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="OQM52_003701"
     CDS             6174..6764
                     /locus_tag="OQM52_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1565391.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="OQM52_003702"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="OQM52_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2168 a         2001 c         2006 g         2188 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cgtttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg cat
//
LOCUS       DAJVQR010000145         8344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVQR010000145 DAJVQR010000000
VERSION     DAJVQR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..171
                     /locus_tag="OQM52_003703"
     CDS             <1..171
                     /locus_tag="OQM52_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565392.1"
                     /translation="RLPQISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHR
                     ERGEDVEPGDDF"
     gene            complement(180..578)
                     /gene="vapC"
                     /locus_tag="OQM52_003704"
     CDS             complement(180..578)
                     /gene="vapC"
                     /locus_tag="OQM52_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCS1565393.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(578..805)
                     /gene="vapB"
                     /locus_tag="OQM52_003705"
     CDS             complement(578..805)
                     /gene="vapB"
                     /locus_tag="OQM52_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCS1565394.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            887..1258
                     /locus_tag="OQM52_003706"
     CDS             887..1258
                     /locus_tag="OQM52_003706"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCS1565395.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1279..6140
                     /gene="traI"
                     /locus_tag="OQM52_003707"
                     /pseudo
     CDS             1279..6140
                     /gene="traI"
                     /locus_tag="OQM52_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6160..6906
                     /gene="traX"
                     /locus_tag="OQM52_003708"
     CDS             6160..6906
                     /gene="traX"
                     /locus_tag="OQM52_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCS1565396.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6961..7521
                     /locus_tag="OQM52_003709"
     CDS             6961..7521
                     /locus_tag="OQM52_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1565397.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7652..7864
                     /locus_tag="OQM52_003710"
     CDS             7652..7864
                     /locus_tag="OQM52_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1565398.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8108..>8344
                     /locus_tag="OQM52_003711"
     CDS             8108..>8344
                     /locus_tag="OQM52_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1565399.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1916 a         2090 c         2674 g         1664 t
ORIGIN      
        1 aggctacctc agatcagtga atccggtgaa gtggtggata tggccgctta tgaggcatgg
       61 cagcaggaaa atcatccgga cacctggcag cagatgcagc gtcgtgaaga ggtgaacatc
      121 aatgtgcacc gggaacgcgg ggaggatgtt gagccgggag atgatttctg atgaacaggt
      181 cagctccagt cttcagttct caggccgccc acacgttcaa attcccgggt gttattagtc
      241 acaataatca gcccccgact gcgggcatga cctgcgatca tttgatcaaa tggcccgaca
      301 gggcgtccct gacgggcaag ttctgctctt atctggccgg tgtgtgtggc tgcagcagcg
      361 tcgtaatcca gaacatcaat gcgggaaaca aatccctcga tcacagcgag attacgttca
      421 ggcatctggc ttttttctgc accatatatc agctccatca gggtgaccga actgatacac
      481 attttcccct ggttcaggtt aaaacgctcc ctgacgctgg cgggtttgtt ctttatcgta
      541 aaaatgcaga tgttggtatc gagcataaac ttcagcatca gaatgactcc ctttcttgca
      601 tgccgggctg ttccctgtta tccataaaat cggtgctgac gctgtgtccg tcgaaccatt
      661 cgtcccatgt ctctccggct ggcgtaatga ttctggttcg tccgacagca atcacttcaa
      721 cgcgttttac gttttccggc aatgcaaccg cttttggcag tctgaccgcc tggctgcggt
      781 tgctgagaaa tacggtggtt tccattattt gcctccttat gtggatatgt cttgtgtata
      841 tctattgtgg gattgatacc gggatatgtc aaagggatat acgtttatga tgagtatcgc
      901 gcaggtcaga tcggccggaa gtgccggtaa ttattatacc gacaaggata attactatgt
      961 gctgggcagc atgggagaac gctgggccgg ccagggggct gaacaactgg ggctgcaggg
     1021 cagtgtcgat aaggatgtct ttacccgcct tctggagggc aggctgccgg acggggcgga
     1081 tttaagccgc atgcaggatg gcagtaacag gcatcgtccc ggctacgacc tgaccttctc
     1141 cgctcccaaa agtatctcca tgatggccat gctcgggggg gacaaacgtc ttattgaagc
     1201 acataaccag gccgtggatt ttgctgtccg tcaggtggag gccagtgcct ccacctgacg
     1261 gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt taaccacgac
     1321 accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa tgttacgcag
     1381 tataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg gttcattgag
     1441 aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact gaaagagcag
     1501 gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg ggaaatgccg
     1561 ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc cgtcggggaa
     1621 gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc caaacagcac
     1681 gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac cgggttcgac
     1741 atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca gacacccgga
     1801 cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat tgccggatta
     1861 agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt cggcatactg
     1921 ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc catcagccgt
     1981 gagcagctta tccccctcga tcgtgagaag gggctgttca cgttcgggat tcatatgctc
     2041 gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg ggtgaccgta
     2101 catccggaga aaagtgtccc ccggacggcc ggttacagcg atgccgtcag tgttctggca
     2161 caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg gcagcgtgag
     2221 cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca gattatcgct
     2281 gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg tgaactgata
     2341 accggacgtc gtcagttgct ggaaggaatg gccttcacgc cgggcagtac cgttatcgtc
     2401 gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg tgccgcacgt
     2461 cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg cagtgcactg
     2521 atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga acagcgaccg
     2581 gccaccatca tcagtgaacc ggaccgtaat gtcggctggc cggagatttt gcggccagcg
     2641 tgaaagccgg agaagagagc gtggcacagg tcagcggagt acgggaacag gccatactga
     2701 cacaggccat tcgcagtgag ctgaaaacac agggcgtgct cggacacccg gaggtaacca
     2761 tgaccgccct gtcaccggtc tggctggaca gccggagccg ttatctgcgg gatatgtacc
     2821 gcccgggaat ggtgatggag cagtggaacc cggagacacg cagtcatgac cgctatgtga
     2881 cagaacgtgt gacggcgcag agtcacagcc tgaccctgcg gaatgcgcag ggcgaaacgc
     2941 aggtggtgcg tatttcttcc ctggacagca gctggtcgct gttccggccg gaaaaaatgc
     3001 cggtggcaga cggtgagcgt ctgagggtga cagggaaaat tcccggactc cgcgtctccg
     3061 gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc gatgacggtt gttgtgccgg
     3121 gacgggctga accggccacc ctgcctgtga gcgattcacc gttcactgca ctgaagctgg
     3181 agaacggctg ggtggaaacg cccgggcatt ccgtcagcga cagtgcgacg gtttttgcct
     3241 ccgtcacaca gatggcaatg gacaatgcca ccctgaatgg tctggcccgc agtggtcgtg
     3301 atgtccggct gtattcctca ctggatgaaa cccgtactgc ggaaaaactt gcccgccatc
     3361 cctcctttac ggtggtttct gagcagataa aggcgcgtgc cggtgagaca ttgctggaaa
     3421 ccgctatcag tctgcagaaa gccgggcttc acacgccggc acagcaggcc attcatctgg
     3481 cccttcctgt gctggaaagt aaaaacctgg ccttcagcat ggtggacctg ctgacagagg
     3541 cgaagtcgtt tgctgcagaa ggaaccggtt ttgctgacct gggaggggaa atcaatgcgc
     3601 agataaaacg gggtgattta ctgtatgtgg atgtggcaaa aggctatggc acaggcctgc
     3661 tggtttcccg tgcgtcgtat gaggcagaaa agagcattct tcgccatatt ctcgaaggta
     3721 aggaggcggt cacgccgctg atggagagag tacctggcga actcatggag aaactgacat
     3781 caggacagcg cgccgccacc cgtatgatac tggaaacgtc tgaccgtttc acggtggtgc
     3841 agggctatgc cggtgtgggt aagaccacac agttccgggc ggtgatgtca gccgtgaaca
     3901 tgctgccgga gagtgaacgt ccccgtgtcg tggggctggg gcccacacac cgtgcggtcg
     3961 gggagatgcg cagcgccggc gtggatgcac agacactggc gtcctttctg catgacacgc
     4021 agctgcagca gcgcagcgga gaaactccgg atttcagcaa cacgctgttc cttctcgatg
     4081 agagctctat ggtgggcaat accgacatgg cacgggcata cgccctgatt gcggccggtg
     4141 gcggtcgggc tgtggccagc ggtgacacgg accagctgca ggccatcgcg cccggtcagc
     4201 ctttccgtct ccagcagacg cgcagtgctg ccgatgtggt catcatgaag gagattgtgc
     4261 gtcagacgcc ggaactgcgg gaggcggtat acagcctgat taaccgggat gtggaaaggg
     4321 cgctgtccgg gcttgagcgt gtgaaaccgt ctcaggtgcc acgtctggag ggcgcatggg
     4381 caccggagca ctccgtgacg gagttcagtc acagccagga agcgaaactg gcagaagcgc
     4441 agcagaaggc gatgctgaaa ggcgaggctt ttccggatgt ccccatgaca ctgtatgaag
     4501 ccattgtccg cgactatacc ggcaggacac cggaagcacg ggagcagacg ctgattgtca
     4561 cgcacctgaa tgaggaccgg cgcgtactga acagcatgat tcatgatgca cgggaaaagg
     4621 ccggagaact ggggaaggtg caggtcatgg tgcctgtcct gaacacagcg aatatacgcg
     4681 acggggagct gcgtcgtctc tccacctggg agaataaccc ggatgccctt gccctggtgg
     4741 ataatgtgta tcaccggatt gccggtatca gtaaggatga cgggctgata accctgcagg
     4801 atgcggaagg taacacgcgg ctgatttcgc cccgggaggc ggtggctgaa ggcgtcacgc
     4861 tgtacacccc ggacaccatc cgggtgggga ccggtgacag gatacgcttc acgaagagtg
     4921 accgggagcg cggttatgtg gccaacagcg tctggacggt gacagcggtt tccggtgaca
     4981 gtgtcacact ttctgacggt cagcagaccc gtgtgattcg ccccggtcag gagcgggcag
     5041 agcaacatat tgacctggcc tatgccatca ccgcccacgg tgcacagggg gcgagtgaaa
     5101 cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa actgatggcc ggctttgagt
     5161 cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggtgtacacc gataaccgtc
     5221 agggctggac ggatgccatc aacaatgccg tacagaaagg aactgcccac gatgtgtttg
     5281 agccgaaacc ggaccgggag gtcatgaatg cagagcggct gttcagtacg gcgcgggagc
     5341 tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc ggggctggcc gggggagaca
     5401 gtcctgcacg gtttattgct ccgggacgta aatatccgca accgtatgtg gcactgccgg
     5461 cgtttgaccg taacggcaag tccgccggta tctggctgaa cccgctgacc acggatgacg
     5521 gaaacgggct gcggggattc agcggtgaag gtcgggtaaa aggcagcggg gatgcgcagt
     5581 tcgtggccct gcagggcagc cgtaacggag aaagcctgct ggctgataat atgcaggatg
     5641 gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt ggtaagaatc gccggtgaag
     5701 gccgtccgtg gaatcccggt gccatcaccg gtggccgcgt gtggggggat atcccggaca
     5761 acagcgtcca gccgggagcc ggaaatggtg aaccggtcac ggcagaggta ctggcacagc
     5821 ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg cgcagatgaa attgtccgta
     5881 aaatggcaga gaacaaacct gacctgccgg acggcaaaac agagcaggct gtcagggaaa
     5941 ttgccgggca ggagcgtgac cgggctgcca taactgaacg ggaagccgcg ctgccggaga
     6001 gtgtactgcg tgaaccacaa cgggtgcggg aagcggtccg ggaagttgcc cgggaaaatt
     6061 tgctgcagga gcgtctgcag cagatggagc gggatatggt tcgtgacctg cagaaagaga
     6121 aaaccccggg cggagactga tacaggaaga caaacactga tgacaacgga taacacgaac
     6181 acgacacgta acgattcact ggctgcacgg accgatacct ggttgcagtc atttctggtc
     6241 tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat ggtactggac
     6301 cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg gcgaggagcc
     6361 tttccgcttt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca tatccggcaa
     6421 ccggccatta accggctgtg gggatgggga attattgccc agttcgcata ttatctggca
     6481 ggctttcctt ggtatgaggg gaatatcctg tttgcctttg cagtggcggc acaggtgctg
     6541 acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccatactcct gatggccctg
     6601 tgggggcctt tgtccggcac cagttacggt attgccgggt tgctgatgct ggcagtcagc
     6661 aaccggctgt accgggcgga agacagagcg gaacgtctgg cgctggtcgc ctgcctgctg
     6721 gctgttattc cggcgcttaa ccttgccacc agtgatgcag cggcggtggc cggtcttgtg
     6781 atgacggtgc tgaccgttgg tctggtgttg tgtgccggaa aatcattacc ccgtttctgg
     6841 cccggagact ttttcccgac gttctatgcc tgtcatctgg ctgtgctggg cgttctggcg
     6901 ctgtgacggg tgtggtatct ttggccgcaa gaggatgatt cgtcagaggc agaacacagc
     6961 atgacagagc agaagcgacc ggtactgaca ctgaagcgga agacagaggg aacagcgcct
     7021 gtccgcagcc ggaaaaccat catcaatgtc accacgccac caaagtggaa ggtgaaaaag
     7081 cagaagctgg ccgagaaagc ggcccgtgaa gcagagctgg cggcaaaaaa agcgcaggcc
     7141 agacaggcgc tgtccattta tctgaacctg cccacgctgg atgaggctgt gaataccctg
     7201 aagccctggt ggccgggatt atttgacggt gacacgcccc ggcttctggc ctgcggtatc
     7261 cgggacgtgt tactggaaga cgtggcgcag cggaatatcc cgctctcgca taaaaaactg
     7321 cgcagggcgc tgaaggccat cacccgttca gaaagctatc tgtgtgccat gaaagccggt
     7381 gcctgccggt atgacacgga agggtatgtg acggagcata tttctcagga ggaggaagcg
     7441 tatgcgggag cgcgtctggc gaaaattcgt cgccagaacc ggataaaggc agaacttcag
     7501 gccgtgcttg atgagaaata aaaaaagcct cccccagaga gaggaggcag ggaaataagg
     7561 cttaaaagga aataacttcc gcttccgaaa ggaagccagt ccactgaatt atttatcaca
     7621 ataatactga caactaaagg gggacaagaa aatgaatgga ttcagaaaca gttcacggaa
     7681 cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc gttattctgg aagtcagtgg
     7741 acgctggatg gaagcggcag aagcatggcg acgggctgcc tgtgttgcgc ctaggacaga
     7801 ctggcagcag tttgcccgga aaagggctga acactgccac cggcgctgcc ggggaagggt
     7861 gtaacgaaaa aagcagcctt cattactcta ggagtcagaa ggctgaaagg agtttatgag
     7921 atttgcttct taatatccgg tcgggcagta tgaccggaca gttctgagta gcacagagtc
     7981 ttttccgcat taagtcgatt ttttatattt tgtgattagt ttcacaaaat aaggtgttat
     8041 tcaatgtgtg ctgcactatt caggggggct gaaatacagg tggcatttat ctgatatgga
     8101 gaataacatg aggaaatata ttccactggc attatttatc ttttcatggc cggtattgtc
     8161 tgcagatatt catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga
     8221 ctcactgaag gcggtgcgga tcaggctggt aaatattgat gcaccggaaa agaaacagga
     8281 ttatggccgg tggtcaacag acatgatgaa atcactggtc gcaggaaaaa cggttaccgt
     8341 caca
//
LOCUS       DAJVQR010000146         8279 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVQR010000146 DAJVQR010000000
VERSION     DAJVQR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            7..657
                     /locus_tag="OQM52_003712"
     CDS             7..657
                     /locus_tag="OQM52_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565400.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OQM52_003713"
     CDS             629..1063
                     /locus_tag="OQM52_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1565401.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OQM52_003714"
     CDS             complement(1836..2885)
                     /locus_tag="OQM52_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1565402.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OQM52_003715"
     CDS             3336..4526
                     /locus_tag="OQM52_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCS1565403.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OQM52_003716"
     CDS             complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OQM52_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCS1565404.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..7302)
                     /locus_tag="OQM52_003717"
     CDS             complement(6373..7302)
                     /locus_tag="OQM52_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1565405.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7299..7661)
                     /locus_tag="OQM52_003718"
     CDS             complement(7299..7661)
                     /locus_tag="OQM52_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCS1565406.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7923..>8279)
                     /locus_tag="OQM52_003719"
     CDS             complement(7923..>8279)
                     /locus_tag="OQM52_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1565407.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT         2531 a         1598 c         1685 g         2465 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc caggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaagg gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa
     6601 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag
     6661 tgccagattc cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg
     6721 tacatattca acgacatccg tcggaccacc cacccagctc agtatgcctt tcatgaaaag
     6781 attgcgctca ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc
     6841 tccgacattt tcctcgatct ttggggtgct tattttgttg tgtaatttat agaaccactc
     6901 agctgtctta cgtttcagtc gtccatcagt tgagcggtct gaacgtttag caagcaccat
     6961 gtcagcacct gcctgccact tttcaataag atgagggata acctcaattg ggtcttgcag
     7021 gtcgacatca ataggaatta cagcatcgcc gcttgcatgg tctaaccctg caaataaggc
     7081 tggttcttta ccaaagttgc gtgtaaatga cagcggaaca actagcgggt ctgaaacagc
     7141 cagcgcgtta ataattgact ctgtggcatc tttacttccg tcatttatga atacaatttc
     7201 tacttcatat ggcttcaact cttggaattc acgtaccgtt ttatagaaaa caggtatcgc
     7261 ttcttcttca ttgaagacag gaacgacaag agatattttc atttcgcatc cctaaagaca
     7321 atgaactttg aatagacgaa accgcacacc agactgatgg cggagaaggt gataagagtg
     7381 actattggag gaagtgagca tttatcagct gcccaaccaa caatcacact caatattccc
     7441 ataaatccca cgtataacat gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt
     7501 gcattcgcga agaagctaaa gctcacagcc actacgaaac ctgtgaagtt tgccagagcc
     7561 tgactggtat gtgcggcata gatacataca ccaaaaacca cccagtgtat aagtgtgttc
     7621 agcacaccta tagatgtgta ctttacaaat aactttaaca tttatttaat caatgagctc
     7681 tgaaaggcat gaagtctatc atccaagtct caattgatcg atacttgcta tgtctgatga
     7741 gacaaaactg agacacataa ggcctcacaa tggcttgcaa ggctttacat gttttgatgt
     7801 ggtgggacgt gtgagcgcag tgttgatggg ataatccttt gaattacaag cggattctta
     7861 taattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt taaatcaata
     7921 agttacctcg catttaagta aactacgttc tcctcttgtg ccgtatttgt gccattgcga
     7981 cttataatcg catcgatttt gctcgcgtgc tcggtaagat gcccggctga aaggtgggcg
     8041 tatctttgaa ccatttcgag agtttcccat cctcccatct ctttaagcgc aagaagagag
     8101 acaccggact gaaccagcca gcttgcccag gtatgcctca ggtcatggaa gcggaagttg
     8161 ctaatgcctg cccgctttaa cgctcccttc catgccttgt tgctgtcggt tctcatcttc
     8221 cttaccgctg ctgtttttgt tccgtcgctt cggtaggcag gtttggtgtg gacaaacac
//
LOCUS       DAJVQR010000147         8237 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVQR010000147 DAJVQR010000000
VERSION     DAJVQR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..249)
                     /locus_tag="OQM52_003720"
     CDS             complement(<1..249)
                     /locus_tag="OQM52_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565408.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(336..697)
                     /locus_tag="OQM52_003721"
                     /pseudo
     CDS             complement(336..697)
                     /locus_tag="OQM52_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1036..1590)
                     /locus_tag="OQM52_003722"
                     /pseudo
     CDS             complement(1036..1590)
                     /locus_tag="OQM52_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1733..1855
                     /locus_tag="OQM52_003723"
                     /pseudo
     CDS             1733..1855
                     /locus_tag="OQM52_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2117..2683)
                     /gene="ipgB2"
                     /locus_tag="OQM52_003724"
     CDS             complement(2117..2683)
                     /gene="ipgB2"
                     /locus_tag="OQM52_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCS1565409.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2965..3279)
                     /locus_tag="OQM52_003725"
     CDS             complement(2965..3279)
                     /locus_tag="OQM52_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565410.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3357..3467)
                     /locus_tag="OQM52_003726"
                     /pseudo
     CDS             complement(3357..3467)
                     /locus_tag="OQM52_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3791..4468)
                     /gene="ospD1"
                     /locus_tag="OQM52_003727"
     CDS             complement(3791..4468)
                     /gene="ospD1"
                     /locus_tag="OQM52_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCS1565411.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4840..4977)
                     /locus_tag="OQM52_003728"
                     /pseudo
     CDS             complement(4840..4977)
                     /locus_tag="OQM52_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5059..5357)
                     /locus_tag="OQM52_003729"
                     /pseudo
     CDS             complement(5059..5357)
                     /locus_tag="OQM52_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5946..6105)
                     /locus_tag="OQM52_003730"
                     /pseudo
     CDS             complement(5946..6105)
                     /locus_tag="OQM52_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6109..7338)
                     /locus_tag="OQM52_003731"
                     /pseudo
     CDS             complement(6109..7338)
                     /locus_tag="OQM52_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7358..7705)
                     /gene="tnpB"
                     /locus_tag="OQM52_003732"
     CDS             complement(7358..7705)
                     /gene="tnpB"
                     /locus_tag="OQM52_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1565412.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7705..8079)
                     /locus_tag="OQM52_003733"
                     /pseudo
     CDS             complement(7705..8079)
                     /locus_tag="OQM52_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2101 a         1922 c         1814 g         2400 t
ORIGIN      
        1 gcgagcgatt accgttgccg ggattttgta aggatgagca tcggcgatgc gttgacgaat
       61 ataatcccgg tattcatcca ggagtgaagc aacagcaggt cgcggcgtat attttggcgg
      121 ctcagatttt gcctgcaaat aacgtttaac ggtattgcgg gagagcccca gttctctggc
      181 aatcgcccgg ctactcattc cctgcttgtg caggatttta atttccataa ctgtctcaaa
      241 agtgaccata agctctcctg aatcaggaga gcagattacc ccctggatct gatttcaggc
      301 gttgggtgtg gatcactatt gcaccgttcg tgacagtgtt gttgcgcctt gctgccaacg
      361 aagctccact gcccatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca
      421 gatgttatcc gccggggcgg gagcttttta atgtccgtat gacggtattg atgccgattt
      481 tcagcgtacg gccagtatcg cgaactccgg caccgttgaa agccatttca gtgatttggt
      541 ttttaactcc cggtttacgg gcctcgtaac agtaagtgag ctgaaacacc cggtgacagt
      601 cacggcaacg gaatcgatca tgtccttttg ggttctgccc gtgacggtaa acctgaacgg
      661 actggcaacg ggggcaatga aagttaactg tgaccatgaa agaacctaaa agcgggtatt
      721 ataaccccta attcaactaa ttggaggcat tgtcattttt aatctatgga tacacttaaa
      781 aatcctctca ccttacgtta aaaaccttct catgtttaga gttttttatt atgtttatca
      841 taatgatatg acgatctgag tttttaaaat caagaagaaa agataagttt tttaaatgca
      901 cagtgcctgt ggataacttt gcgtaaatcc ggaggacaga tcactctgaa gcaagacaac
      961 agcatctgaa aacaatatgc tcacgtttca accacagggc gtaagtgatc ctacccacgt
     1021 aatatggaca caggcctaag cgaggttctg gttttcaaat tgttccggac tgaggccgcc
     1081 acaccaactg tgccgtcgcc agcgattgta atcacattcg atataattaa acactgttgc
     1141 cctcattatt tcccggctga taaagtgttc tccgtggaga cattccactt tcagcgaatg
     1201 aaagaagctt tccacgcagg cattatcgta gcagcagccc tttgcgctca tactgccacg
     1261 cagattatgc cacttcagca gcgcctgata atctgctgaa cagtactgac ttcctcggtc
     1321 cgagtgaacg ataacgttcc ggggtctctt acgccgccac agcgccatct gcagggcatc
     1381 gcaggccagt tgcgccgtca ggcgtggtga catggaccag ccaataacgg cacgtgacca
     1441 caggtcaatg accacagcca gatacagcca gccttcatct gtacgtaagt acgtgatgtc
     1501 tcccgcccac ttctggttcg gaccactggc gtaaaaatcc tgctccagca gattttctga
     1561 cacaggcagg ccatgtgcgc ggtaactgac cgtattgctg ggtaagatca atttgctcac
     1621 tggtatttac tacgtgcatc gaatgagtga tcaagagccg tatgacgcga gagtgtcacg
     1681 tacggttccg taagcagccg gagagtgaaa ctctctccgg ctgactcgac tcgcaggttc
     1741 cgacagcggt gacgaacatg cagcggtgtt gttctcgctg atcggcacat gccgtctgaa
     1801 caatgtggag ccagaaaaat ggctgcgtta cgttattgaa catatccagg actgaccggc
     1861 aaaccgggta cgcgatctgt tgccttggaa agttgatctg acttctcagt aaatatcaat
     1921 atgattctga cgagccgctt acctttgagc agagaaaggg gagagggatc ccacatccat
     1981 gagctaggaa aatacaaaaa ctggctatac aacaattcaa tatattagga cttacttgcg
     2041 aggagaaata tagcaccgct caggcaattc ctgggtattt acttttctta gtttctttac
     2101 tcgggatgtt ctgtcatcag aaaggcgatt ctaaatttgt aatatagtca ctcatattcc
     2161 cacgtaattt ctttagatca atatattgca catttttctc aaaaaactga tcggaaataa
     2221 gatttgcaac attgcttgtt gtatcatttt taacgcttct attcatccct tcacaaagtt
     2281 cgtccatttt ctttttgaaa taactgcttc ctgatacaag atgatttatg gaactttgtg
     2341 cagcattgat gtctagagaa atgcgataat ctttctcaac ctgtttaaat atctgtttac
     2401 ggagattatc gccgattttt cggctgtgtt ctgcacaaaa cttatcaata cattggttta
     2461 ccacccggga tacgtagact tttttttcat catttttcca atctgtaata ttttcaccga
     2521 tttgttctga aacagagctc aaaatactag tatttatttt ggttgatgat atttttacta
     2581 tccttttccc cattgtacag cggataatct catccacctt tcctgaaaaa tatcttttgt
     2641 gagataaact gattccaaaa ttattaaaag atgttccaag cattataatc accttgtcat
     2701 gtaaaatagt aacattgtca ttattttagc tgattcaaac aattctgtaa atgctttgag
     2761 ttctttactc ccttggctca atgatggacc gtctggaatc ccagagacta aactctctga
     2821 gaaagagagt atgaaaaaac ttggggcagt ccctaaatga agggaccaac agcggtaaaa
     2881 taaccggcat cattcataat gatgccctgt tagttgtagt tatgaagatt tacgtcatta
     2941 aaattgctgt tcgtagtgtc agtcctatgg tctggcatca gctgaggatc gtagccgata
     3001 catcactggc ttcacgacat tcgtgttgag gctattcatg aaaactccac gctgaaaacg
     3061 ccgttttgta taaacggtca cggtatgccc ggagccactg ttgctgataa attcgataaa
     3121 accttggcgt ttcttgaagc tatcgttaat gcggataatg aaacaacgat tggtgagatt
     3181 cgctcttttg ccgacgccct ggacgctgtc cggttcaatc gcaacaaaat caaccggcag
     3241 ctgagcaaac ccaaccttgc ctctctggcg ctggagcatg aagtgatttg gctgggacgt
     3301 agccgctgac agcttctata aaagtatgat cctacccacg taatatgaac acaggcttaa
     3361 gtgaggttct ggttttcaaa ttgttcagga ctgagacctc cacaccaact gtgccgtcgt
     3421 cagcgattgt aatcgcactt gatataatta aacactgtat atttcatttg tggagtctcc
     3481 agtctgagag ggctttatgc attccgtatt gggcactatg atgccagaaa tctgcaaggc
     3541 tccacacctt cctgtatttc ccttcctcta tcacagtgca ctttacttaa tagggtggga
     3601 tgtgttcatt acatttattg tgttttttat gattgttttt gcgtccagtg tcgttcgtca
     3661 tgggaaactg gttctatggg tagtatcgct cagtctagtt aactgtttgt ttctttttag
     3721 aaattaagca gatcacacaa ttcggggaga gatccttcta agtgcaattc tttgcagcta
     3781 tttagcacac ttaaaacaac tcttgtattt tgtcagaaat aatattaaac atgtcttgtt
     3841 cattattgtt aagtctgtct gctatttctg taagttttat cccactatga tggcatatat
     3901 ttatatatgc catcatagcc tcgatattcc ctttccctgc ggccagaaat aatcctggta
     3961 acccctcatt atctcttaca tccaatagtt taatcgtttc ttttggtgat aaatcaaaag
     4021 ttttaataaa atctccataa gcttgaatcg cagcggaatg accttcttga agagctatga
     4081 acaaagcaga ttccccagca ttatttcgtg ctgcaagaac ttctagtttt tcctctttgg
     4141 gtatttcggc tgccaacagc gattcaccgt atttacgcgc tgcatcggca tttccatgcc
     4201 aaatcaatga aaacaaatta tggcggttat actccttatt attctttgtc ggaatggata
     4261 tgttctgtat tttggccaat tttctgaaag aaacaccact atatcccccc cctgattttt
     4321 cttcattgat ggcgtgggat atagcggtat ttgtcacgtt aatgatatta tttttcattc
     4381 ctttattttc attagcagta ttgctgccta ttattaagtg catatttttg ttgtttgctg
     4441 gatgtaatcc atagttattt attgacattt ttgttcatcc tttttgctaa aaaaaaacaa
     4501 taacctattt ttgtaatgta atagatatta gtctattgct aatctaaaaa aaccattgcc
     4561 acattgaatg gctatattca ccaataaatt ctttcgattt cgtatattga catatcgtag
     4621 atatatctta tccctaagat tcaataatcg agtccgttta ccattctgtg caagcgcctt
     4681 cactcagaag tgaccgtcaa ggcgatcacc aaacttgata ataaagcagc tcattgccat
     4741 acgccagtcc ttaaggggcg ttgtccattt ctgtgatgct gcctagattt aactgccagc
     4801 tccggcttat ctacatctaa atatgtgagc ctaataccgc tacactttgt ccagactatg
     4861 tttttctccg ggaagtgggc taaccgtctc cataaagtga cgaacttttt tcaacagtgg
     4921 ccatattgaa cggcactgat gattacgggt tattgtctca tgaagagccg gccacatcac
     4981 tgacagcagt gacttcaggc cttcagcacc agacagcctg aaccagttaa tccatcgccc
     5041 gacaaattta gtaatctagg gctggttttt cttcagctgt tgcggttcga aagtgccatt
     5101 acgatcacgc agagtacgga tttccagagg ttcatctcca gtagtgataa tcttcgttaa
     5161 gcaaccattg cgggaattgc tgccagatct aagctgattt ttctcgcacc caagatgatg
     5221 agtcatatta gcattgagta cggtttcatt ttcagcatcc aatcaaactg actgagatct
     5281 tcaggatttt tgagactttt ggctagttca ttagctaaag cccgcaactt attagatgtc
     5341 aactgttttt cattcataaa ttcaccttca tttgatatca aattaaatag ttcaacatca
     5401 gagaattata caaacttatg tacaggctcc tatgatcttc gaatcctgaa gattatagtg
     5461 aaaataagtt ttcacgttga aatcccacat agttagtata tgataaataa tcatggatga
     5521 cctgattaac ctcttggggt agtgctttta tagaagaata aatattctac taatttaata
     5581 catcacctaa aatagtattg ttttttatgg ttggttgaag gtcgtggtga tgatgttagt
     5641 ccttttgaga atattccagt gccagatctt acttataata aaggggcttg gcagtttgga
     5701 taaactattg ttcttattcc actaatacaa actttttgta aagtatatta aatatacttc
     5761 atttcatact gaaatccccg gaagtattca atcaaacaat tacaccataa aaggaagagg
     5821 gggccctaat taagttgtca agcatgttta tgatacctgc agggtataaa ttttctggct
     5881 gcaagcagac acatataaaa cgtctttatt gatcctaccc acataatatg gacacagtcg
     5941 taagtgaggc tctggttttc aaattgttcc ggactgagac caccacacca acggtgccgt
     6001 cgccagcgat tgtaatcgca ctcgatataa ctaaacactg ttgcccgcac tatttcccga
     6061 tggataatag tgttctccgt ggagacattc cattttcagc gaatgaattt tcatctgttg
     6121 ctgtatccag tcatacagtg actgcatcag tggcgcggct ctggcttttc ttgccgccag
     6181 acgctgttct gctgaacatc cccggacttc tgcctcgatg gcatacagtt caccgatacg
     6241 ctgcagggct tccgtggtga tgtcggttgg cactcttgca tgcacatcgt ggatttttct
     6301 ccggacatga gccatacatg cggcttccgt tattctgccg gattcgtaca acgcccggta
     6361 accaccgtaa gcatcggcct gaagcactcc gctgtaaccg gccagatgat tctgtggatg
     6421 gatacctttc cggtccggtg agtacgcgaa ccagaccgcc ggaggcattt ccgaaccggc
     6481 gttacggtca tcacgaacgt agacccacag ccgggcagtc cttgttttac cgctgcccgg
     6541 ctcctggacc ggaacgggga tatcatcggc atggacttta ccgggcatca gcacatactg
     6601 gcgcaggatg tcatacagcg gttccagcag ttcagcaaca gcacctgtcc agcgccccag
     6661 tgtggcacgg ctcagctcca cgccctgacg acggtatatt tctgactggc ggtataacgg
     6721 cagatagtct gcatattttc cagcaacaac atgggccaga agccccgctc cggcataact
     6781 gcgtgcaatg ggttttgaag gcaccggtgc ctgcacgata tggtcacacc ggcaacaggc
     6841 cagtttcgga cgttgtgttt cgataacctt aaaggcgctg ctgataagct ccagctgctc
     6901 tgacacatca catcccagag aactgagttc gccaccacag gccgggcagc attcctcttc
     6961 cggacggatg acccgggttt cacggggaag tgaggctggt aacggtttac gggctgaaga
     7021 ctggcgcagg ggagatggca gtaccgggtc atattgctca cccagcgttt ccgccatttc
     7081 ttcctgaagt gcgctgattc gctcctgtgc ttccagtatc tgccgttcgg tttttgcgcg
     7141 aagtttttct gagcttttac cgaactgcat gcgctgcagt ttcgcaacca gcgccttcag
     7201 ccggttgatt tcggaagcat aagccgccac ccgctgtgag agcaggcggt tgtatttcgc
     7261 catctggcgg atggtgtcct gttgcgtctg caacagtgcc cgcaggcggg cattctcatg
     7321 agcaagtgag gtgtccatat cctcacttta caacgggtta tatgcggatg ccagcgcgtt
     7381 ccgttcgttt cgggtgtttc cagtcgatgc cctcaagaag catggataac tgggccggag
     7441 taaggtgcac cttgccgtca cgggtgaccg gccagacgaa gcggccccgc tccaggcgtc
     7501 tggtaaagag gcacagtccg tcactgtcag cccacaacac ttttatctgg tcaccccggc
     7561 gtccgcggaa gatgaacaga tgcccggaga acgggtcatc cttcaggacg ttctgaactt
     7621 ttgagaccag gccgttaaag ccatttcgca tatcggtgat acctgcaacc agccagatac
     7681 gcgaacctgc agggaaagat atcatcagtg gctgctccct tttatttcgc ggataagtat
     7741 ctgtaataac gccggcgtca gtttaccttt aagcctgaga gttccggccg gcagaaccag
     7801 ctcacagcac agactgtcgg acggtgtatt tatctgctct ggttcctgtg ccggagccgg
     7861 gattttatta tccggctccg gcgttaacgt cacgggaagc agtgcaggca tattttttcc
     7921 ggaaggcagc aggccacctt tccggtattg atggcgccag ttgaagagca ggttatcgtt
     7981 gattccgttt tcacgggcga tctgcgccac acaggctccg ggctgcagtg actgctccac
     8041 taaagcgatt ttaaattcat aaggaaaatt aggccgccgg tgtaatgccg atcagttaag
     8101 gatcagttga ccgatccagt ggctgtgtaa gaatccggaa acgctcactt gtttccggat
     8161 tcttttatgc acattggaca ggctcttgat ctggtatccc gttacgattc tctgcgtaac
     8221 ccactgactt ctctggg
//
LOCUS       DAJVQR010000148         8224 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVQR010000148 DAJVQR010000000
VERSION     DAJVQR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..305
                     /locus_tag="OQM52_003734"
     CDS             <1..305
                     /locus_tag="OQM52_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCS1565413.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2187
                     /locus_tag="OQM52_003735"
     CDS             277..2187
                     /locus_tag="OQM52_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS1565414.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2188..2391
                     /locus_tag="OQM52_003736"
     CDS             2188..2391
                     /locus_tag="OQM52_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565415.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="OQM52_003737"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="OQM52_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="OQM52_003738"
     CDS             3971..5476
                     /locus_tag="OQM52_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS1565416.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="OQM52_003739"
     CDS             5513..5860
                     /locus_tag="OQM52_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1565417.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="OQM52_003740"
     CDS             5918..6946
                     /locus_tag="OQM52_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1565418.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="OQM52_003741"
     CDS             6998..7384
                     /locus_tag="OQM52_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1565419.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="OQM52_003742"
     CDS             7396..7773
                     /locus_tag="OQM52_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565420.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..>8224
                     /locus_tag="OQM52_003743"
     CDS             7760..>8224
                     /locus_tag="OQM52_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565421.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1905 a         1932 c         2540 g         1847 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagcttg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgca gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgtgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac cagggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtattttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgcagttgca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ctaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgcgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 agctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagttctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggctgcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacggcccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat
     8221 cccg
//
LOCUS       DAJVQR010000149         8115 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVQR010000149 DAJVQR010000000
VERSION     DAJVQR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            6..290
                     /locus_tag="OQM52_003744"
     CDS             6..290
                     /locus_tag="OQM52_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1565422.1"
                     /translation="MLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDVSTQSSPVEM
                     NCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEYN"
     gene            385..918
                     /gene="ymdB"
                     /locus_tag="OQM52_003745"
     CDS             385..918
                     /gene="ymdB"
                     /locus_tag="OQM52_003745"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1565423.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            860..2341
                     /gene="clsC"
                     /locus_tag="OQM52_003746"
     CDS             860..2341
                     /gene="clsC"
                     /locus_tag="OQM52_003746"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1565424.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2349..3506)
                     /gene="mdoC"
                     /locus_tag="OQM52_003747"
     CDS             complement(2349..3506)
                     /gene="mdoC"
                     /locus_tag="OQM52_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1565425.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3900..5435
                     /gene="mdoG"
                     /locus_tag="OQM52_003748"
     CDS             3900..5435
                     /gene="mdoG"
                     /locus_tag="OQM52_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1565426.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5428..7971
                     /gene="mdoH"
                     /locus_tag="OQM52_003749"
     CDS             5428..7971
                     /gene="mdoH"
                     /locus_tag="OQM52_003749"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1565427.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2028 a         1939 c         2132 g         2016 t
ORIGIN      
        1 ctcttatgct cggcagtttg ttttttcctt ttattgccat tgcaggaagc accgcgcaag
       61 ggggcgtgat ccatttttat ggccaaattg tggaaccggc atgtgacgtc agcacccagt
      121 catcacccgt agaaatgaac tgcccacaga atggttctat tccgggcaaa acctactcca
      181 gcaaagcgtt aatgagcggc aatgtcaaaa acgcgcaaat agcctcagta aaagtgcagt
      241 atcttgataa acagaaaaag ctggctgtaa tgaacatcga atataactaa gtttctgaac
      301 aactcacgtt gctgagcaga aaaatgcgat ttaaccaaaa agcctgctgt acacttaaga
      361 aacaagaagg tgtaaggagg cgttatgaaa acgcgtattc atgttgtgca gggtgatatt
      421 accaaactgg ccgttgatgt gattgtgaat gcggctaatc cgtcattaat gggaggcggc
      481 ggcgtcgatg gggccattca tcgcgcagcg ggtccggccc tgctggatgc ctgtttaaaa
      541 gtcaggcaac agcagggcga ttgccctacg gggcatgccg ttattacgct tgcaggcgat
      601 cttcccgcta aagccgtagt acacaccgtc gggccagtct ggcgtggtgg ggaacaaaac
      661 gaagaccagc ttttgcagga tgcctatctg aatagcctac gactggtggc ggcaaacagc
      721 tatacgtcag tggcttttcc tgcaatcagt actggggttt atagttaccc tcgtgcggca
      781 gcggctgaaa tcgcagttaa aaccgtttca gaatttatta cccgtcacgc tttacccgaa
      841 caggtatact ttgtctgtta tgatgaagaa aacgcccacc tctacgaaag actccttacc
      901 caacaaggag atgaatgatt tgccccggct ggcgagcgcg gtgctgccac tgtgttcgca
      961 acatcccggt cagtgtggtc ttttccctct ggagaaaagt ctggatgcgt ttgccgcccg
     1021 gtatcgtctg gccgaaatgg cagagcatac gctggatgtt cagtattaca tctggcagga
     1081 cgatatgtcg gggcggttac tgttttccgc cctgttagcc gcagcaaagc gtggcgttcg
     1141 cgtccgtttg ttgctggacg acaacaatac gcccggactt gacgacattt tgcgcttgct
     1201 tgacagtcat ccacgcattg aagtccggct ttttaatcct ttctcgtttc gcttgctacg
     1261 tccgcttgga tatatcaccg acttttcccg tctcaatcgc cggatgcaca ataaaagttt
     1321 cactgtcgat ggcgtggtta ccctagtggg aggacgaaat attggtgatg cctattttgg
     1381 tgcaggggaa gagccacttt tttcggattt agatgtcatg gcaataggcc ccgtggtaga
     1441 ggatgttgcc gatgatttcg cccgctactg gtattgcaaa tcggtttcac ccttacagca
     1501 ggtgctggat gtcccggaag gtgaaatggc ggatcgcatc gagttacccg cctcctggca
     1561 taacgatgcc atgacgcatc gttatttacg caaaatggaa tccagtccat ttataaatca
     1621 tctggttgat ggaacgttgc cgcttatctg ggcgaagaca cgtttattaa gtgatgatcc
     1681 ggcgaaaggg gagggcaagg caaaacggca ttcactgtta ccgcagcgcc tgttcgatat
     1741 catgggctca cccagtgaac gcatcgatat tatctcttcc tattttgtac cgacacgcgc
     1801 aggtgtggcg caactcttac ggatggtgag aaaaggggta aagattgcga tcctaaccaa
     1861 ttctcttgcc gctaacgatg ttgctgtcgt ccatgccgga tacgcgcgct ggcgcaaaaa
     1921 attgctccgc tatggcgtgg aattatatga actcaagccg acgcgtgaac aaagcagtac
     1981 gttacacgat cgcggcataa ccggtaattc cggtgccagc ctgcatgcta aaacttttag
     2041 catcgatggt aaaacggtgt ttatcggctc tttcaatttc gatccgcgtt caacattgct
     2101 caatactgaa atgggcttcg tgatagagag cgaaacgctg gcacagttaa ttgataaacg
     2161 ctttattcag agccagtacg atgcggcctg gcagctccgt ctggacaggt ggggacggat
     2221 caactgggtt gatcgtcatg caaagaaaga ggttattctc aaaaaagaac ccgccaccag
     2281 tttctggaag cgggttatgg tcagactggc gtcgatattg cccgtggaat ggttattgta
     2341 aatgtggctt aacgggctgg tgctttatcg ttctcacgct taacaaccgg tttaccagag
     2401 aacaaaaact tcagtaacgg gatgcgtaaa tgaatttcat acagaattat cgcaatccct
     2461 actacgaata tcaggccaca gagaaaacca agccagttgg aggtgatgtg cggtgtaatg
     2521 tatgcgccga aaaacagcgt taacgggtgg tgaaccagat agataaacag cgatgcgtta
     2581 acaaaataag tcacccgcgc tgactggaag ttaagcaaac ggtggccaaa ggagaagacc
     2641 acattcacca tccacaggcc gaggaccatg gtgatcaccg actcggtttc gtacatccag
     2701 gcatcgccac tgccatagcg ctggttgagt aaataagcga caaacgccaa tgctgcagcg
     2761 agggtacagc cacgagacgg cgtggtaaac aaggctttaa gatgagggaa aatgaaagcc
     2821 agtgcgccga ggataaagaa cggtaaataa aatagcgttt gcatgacaat aaaattgaac
     2881 atgccattac tcagaatggg tggatacaca ataaaaatcg ttcttcttat taccgcatag
     2941 ccgatgccga ggcataaaaa aatcaccgat agttttacca tcgagaattt tttattcgtt
     3001 ttatcagaat tttctaaatt atttctgatg cgcttaaata tccatacaca cagcgtcgtc
     3061 atgactacta acaccagtaa aaaccacagg tgtgatatta attcccaggc caacgtatta
     3121 tatttgtcat acaatgatag cccaggccaa ctttccgctt tccctttgac atattgcagc
     3181 ataataaatt gcggtaatgt cagtaggggg atggctgtta acatcgggat acctacacgt
     3241 tcgacacgta ctttccacca ttttttcaag ggatagcgta aaaaaagcat gtaggaaaag
     3301 tatccggata taacgaaaaa tacctgcatg cggaacgagt ggatgaagtc attaaaaagg
     3361 gttagccaca atgacggttc ggcgctattc acatgccatg tatggctcga atagattaaa
     3421 gaaatatgaa aagggatccc taacaacatc agccaggcgc ggatggagtc gaggaaatat
     3481 tcacgttgcg cgggtactgg attcatatat ggttaactaa tctcggattt ttcgtcttat
     3541 ccctgtcggg ttatgccttt aggcttgttg ccatagcgac accgacctga ccgcgccagg
     3601 cgcaggcttc aaggttttta tgcatagcat catcgctacc actaaccaga atggaagcgt
     3661 ctgtaagacg gttgataaat aaatttgctg gcaaacccta cacgaagtcg atgcttctgt
     3721 ctttaggaga agcacggaaa gtgaaaacgg ttgcaatcag gtgcttaatc catgagccag
     3781 cgtgctgaac gataccggga ttcagttgtc ggaatggctg gttatccatt aaaatagatc
     3841 ggatcgatat aagcacacaa agggggaagt gcttactaat tatgaaacat aaactacaaa
     3901 tgatgaaaat gcgttggttg agtgctgcag taatgttaac cctgtataca tcttcaagct
     3961 gggctttcag tattgatgat gtcgcaaagc aagctcaatc cttagccggg aaaggctatg
     4021 aggcgcctaa aagcaacttg ccctccgttt tccgcgacat gaaatacgcg gactatcagc
     4081 agatccagtt taatcatgac aaagcgtact ggaacaatct gaagacccca ttcaaactcg
     4141 agttctacca tcagggtatg tacttcgata ccccggtcaa aataaatgaa gtgactgcca
     4201 ccgcagtcaa acgaatcaaa tacagcccgg attatttcac tttcggcgat gttcagcatg
     4261 acaaagacac ggtaaaagac cttggttttg ccggttttaa agtgctttac ccgatcaaca
     4321 gcaaagataa aaacgatgaa atcgtcagca tgctcggggc cagctatttc cgcgtgattg
     4381 gtgcaggtca ggtttatggc ctttctgccc gcggcctagc aattgatacc gctttgccat
     4441 cgggtgaaga atttccacgc ttcaaagagt tctggatcga gcgtccaaaa ccgactgata
     4501 aacgtttaac catttatgca ttgcttgact cgccgcgtgc gacaggtgct tacaaattcg
     4561 tggttatgcc agggcgtgac acggttgtgg atgtgcagtc gaaaatctat ctgcgcgata
     4621 aagtcggcaa actgggggtt gcaccgttaa ccagtatgtt cctgtttggg ccgaaccaac
     4681 cgtcgcctgc aaataactat cgtccggagt tgcacgactc taacggtctg tctatccatg
     4741 ctggtaatgg cgaatggatc tggcgtccgt tgaataaccc gaaacattta gcggtcagca
     4801 gcttctccat ggaaaacccg cgaggctttg gtctgttgca acgaggtcgt gatttctccc
     4861 gctttgaaga tctcgatgat cgttacgatc tccgtccaag cgcatgggtg actccgaaag
     4921 gggagtgggg caaaggcagc gttgagctgg tggaaattcc aaccaacgat gaaaccaacg
     4981 ataacatcgt cgcttactgg acgccggatc agctgccgga gccgggtaaa gagatgaact
     5041 ttaaatacac catcaccttc agccgtgatg aagacaaact gcatgcgcca gataacgcat
     5101 gggtgcaaca aacgcgtcgt tcaacggggg atgtgaagca gtcgaacctg attcgccagc
     5161 ctgacggtac tatcgccttt gtggtcgatt ttaccggcgc agagatgaaa aaactgccag
     5221 aggatacccc ggtcacagcg caaaccagca ttggtgataa tggtgagata gttgaaagca
     5281 cggtgcgcta taacccggtt accaaaggct ggcgtctggt gatgcgtgtg aaagtgaaag
     5341 atgccaagaa aatcactgaa atgcgtgctg cgctggtgaa tgccgatcag acgttgagtg
     5401 aaacctggag ctaccagtta cctgccaatg aataagacaa ctgagtacat tgacgcaatg
     5461 cccatcgccg caagcgagaa agcagcattg ccgaagactg atatccgcgc cgttcatcag
     5521 gcgctggatg ccgaacaccg cacctgggcg cgggaggatg attccccgca aggctcggta
     5581 aaggcgcgtc tggaacaagc ctggccagat tcacttgctg atggacagtt aattaaagac
     5641 gacgaagggc gcgatcagct taaggcgatg ccagaagcaa aacgctcctc gatgtttccc
     5701 gacccgtggc gtaccaaccc ggtaggccgt ttctgggatc gcctgcgtgg acgcgatgtc
     5761 acgccgcgct atctggctcg tttgaccaaa gaagagcagg agagcgagca aaagtggcgt
     5821 accgttggta ccatccgccg ttacattctg ttgatcctga cgctcgcgca aactgttgtc
     5881 gcgacctggt atatgaagac cattcttcct tatcagggtt gggcgctgat taatcctatg
     5941 gatatggttg gtcaggattt gtgggtttcc tttatgcagc ttctgcctta tatgctgcaa
     6001 accggtatcc tgatcctttt tgcggtactg ttctgttggg tgtccgccgg attctggacg
     6061 gcgttgatgg gcttcctgca actgcttatt ggtcgcgata aatacagtat atctgcgtca
     6121 acagttggcg atgaaccatt aaacccggag catcgcacgg cgttgatcat gcctatctgt
     6181 aacgaagacg tgaaccgtgt ttttgctggc ttgcgtgcaa cgtgggaatc agtaaaagcc
     6241 accgggaatg ccaaacactt tgatgtctac attctgagtg acagttataa cccggatatc
     6301 tgcgtcgcag agcaaaaagc ctggatggag cttatcgctg aagtcggtgg cgaaggtcag
     6361 attttctatc gccgccgccg tcgccgcgtg aagcgtaaaa gcggtaatat cgatgacttc
     6421 tgccgtcgct ggggcagcca gtacagctac atggtggtgc tggatgctga ctcggtaatg
     6481 accggtgatt gtttatgcgg cctggtgcgc ttgatggaag ccaacccgaa cgccgggatc
     6541 attcagtcgt cgccgaaagc gtccggtatg gatacgctgt atgcgcgctg tcagcagttc
     6601 gcgacccgcg tgtatgggcc actgtttaca gccggtttgc acttctggca acttggcgag
     6661 tcgcactact ggggacataa cgcgattatc cgcgtgaaac catttatcga gcactgcgca
     6721 ctggctccgc tgccgggcga aggttccttt gccggttcaa tcctgtcaca tgacttcgta
     6781 gaagcggcgt tgatgcgccg tgcaggttgg ggggtctgga ttgcttacga tctcccgggt
     6841 tcttatgaag aattgccgcc taacttgctt gatgagctaa aacgtgaccg ccgctggtgc
     6901 cacggtaacc tgatgaactt ccgtctgttc ctggttaagg gtatgcaccc ggttcaccgt
     6961 gcggtgttcc tgacgggcgt gatgtcttat ctctccgctc cgctgtggtt tatgttcctc
     7021 gcgctctcta ctgcattgca ggtagtgcat gcgttgaccg aaccgcaata cttcctgcaa
     7081 ccacggcagt tgttcccggt gtggccgcag tggcgtcctg agctggcgat tgcacttttt
     7141 gcttcgacca tggtgctgtt gttcctgccg aagttattga gcattttgct tatctggtgc
     7201 aaaggaacga aagaatacgg cggcttctgg cgcgttacat tatcgttgct gctggaagtg
     7261 ctgttttccg tgctgctggc tccggtacgc atgctgttcc atacggtctt cgttgtcagc
     7321 gcgttccttg gctgggaagt ggtgtggaat tcaccgcagc gtgatgatga ctccacttca
     7381 tggggcgaag cgttcaaacg ccacggctca cagctgctgt tggggttagt gtgggctgtt
     7441 gggatggcgt ggctggatct gcgtttcctg ttctggctgg caccgattgt cttctcgttg
     7501 atcctgtcac cgtttgtttc ggtgatttcc agccgtgcca ccgttggtct gcgcaccaaa
     7561 cgctggaaac tgttcctgat cccggaagag tattcaccgc cgcaggtgct ggttgatacc
     7621 gatcggttcc ttgagatgaa tcgccaacgc tcccttgacg atggctttat gcacgcagtg
     7681 tttaacccgt catttaacgc tctggcaacc gcaatggcga cagcgcgtca ccgcgccagt
     7741 aaggtgctgg aaatcgcccg tgaccgccac gttgaacagg cgcttaacga gacgccagag
     7801 aagctgaatc gcgatcgtcg cctggtgctg ctaagcgatc cggtgacgat ggcccgtctg
     7861 catttccgcg tctggaattc ccctgaaaga tattcttcat gggtgagtta ttacgaaggg
     7921 ataaagctca atccactggc attgcgtaaa ccggatgcgg cttcgcaata aaaacgtagt
     7981 tgcctgatgc gctacgctta tcaggcctac atcgttcctg caatttattg attttgcaag
     8041 attttgtagg tcggataagg cgttcacgcc acatccggca aaaacaacga gccaataaaa
     8101 ataccggcgt tatgc
//
LOCUS       DAJVQR010000150         8003 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVQR010000150 DAJVQR010000000
VERSION     DAJVQR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..1322
                     /gene="yjeM"
                     /locus_tag="OQM52_003750"
                     /pseudo
     CDS             1..1322
                     /gene="yjeM"
                     /locus_tag="OQM52_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1473..4784)
                     /gene="mscM"
                     /locus_tag="OQM52_003751"
     CDS             complement(1473..4784)
                     /gene="mscM"
                     /locus_tag="OQM52_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1565428.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4806..5774)
                     /gene="psd"
                     /locus_tag="OQM52_003752"
     CDS             complement(4806..5774)
                     /gene="psd"
                     /locus_tag="OQM52_003752"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1565429.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5871..6923)
                     /gene="rsgA"
                     /locus_tag="OQM52_003753"
     CDS             complement(5871..6923)
                     /gene="rsgA"
                     /locus_tag="OQM52_003753"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1565430.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7018..7563
                     /gene="orn"
                     /locus_tag="OQM52_003754"
     CDS             7018..7563
                     /gene="orn"
                     /locus_tag="OQM52_003754"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1565431.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7774..7849
                     /locus_tag="OQM52_003755"
     tRNA            7774..7849
                     /locus_tag="OQM52_003755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7807..7809,aa:Gly,seq:gcc)
BASE COUNT         1827 a         2069 c         2168 g         1939 t
ORIGIN      
        1 gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg gatgaataat
       61 agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc ttatatcatc
      121 tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct ctatggtagt
      181 gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt tggtctgctg
      241 gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa taaaattgcc
      301 cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt gctgttagta
      361 agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa tttccttgca
      421 tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt atttgctatt
      481 tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga aaatccagaa
      541 aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg ttattcgctg
      601 gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa tggttccgtt
      661 aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct gggtaacgca
      721 ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg tattaccgga
      781 ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa gccatcatcc
      841 aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg atggggatgc
      901 cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg ctggtttcgt
      961 ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg aacgtgtcta
     1021 tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca cgtcaggatc
     1081 tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg acggtggttg
     1141 tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg gttgaagccg
     1201 gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg ctgttagcga
     1261 tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta gctctcgact
     1321 gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag gaagtaatgc
     1381 gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc tgctaaatga
     1441 gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg acgacctttg
     1501 cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag acgcatctgg
     1561 aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat cagctggtgg
     1621 atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa
     1681 atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt gtcgatcacc
     1741 agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact gttggcatcg
     1801 gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga ccagttgata
     1861 aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc ccagtcgctg
     1921 atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg gatcgttacc
     1981 gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat aaagttggcg
     2041 aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag ccactgcaat
     2101 ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag catcagcaga
     2161 tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag gtgctgcaaa
     2221 atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat gataaatacc
     2281 agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac gccctgtacc
     2341 gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga atggatttct
     2401 gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg caccagccgc
     2461 aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc aattgctcct
     2521 tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc taacatctct
     2581 gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag catccagcgg
     2641 cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt ttcaagcctc
     2701 gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat ggcaaccagt
     2761 ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc gctgccttct
     2821 ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac cgccagcgca
     2881 ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg gtcgtcgagg
     2941 ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta gcgcatccca
     3001 cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt cgggcgggca
     3061 aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac accatcgcca
     3121 atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag cgtcatccac
     3181 agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa cgtcagccag
     3241 aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa acgggtgaaa
     3301 tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag cggcaaaatt
     3361 gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa ggtgtccagc
     3421 gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt catcgggcgc
     3481 acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt cagcgcatcc
     3541 tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt gtcgccaccc
     3601 tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag aatacggttt
     3661 tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag cggctgttta
     3721 ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc ggtatcaagc
     3781 tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc ttcgccgagc
     3841 aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc ctgccggacc
     3901 tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat ccgctgcgcc
     3961 tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac gatatctttc
     4021 ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg ctccgcctca
     4081 agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc cagttgctgg
     4141 ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt attggcagac
     4201 agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc agactgcaac
     4261 gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag gcggcgctcg
     4321 atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt cagcgaatcg
     4381 gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc cagcaactgg
     4441 ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg cgacacgctg
     4501 cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag tttcggataa
     4561 ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg ttcctcaagc
     4621 gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt cgccgctttt
     4681 gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc gtacgccccc
     4741 caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc gtttcctttg
     4801 atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg ttctgcttcg
     4861 atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt ttcggtagat
     4921 actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc aaccagattc
     4981 actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg acccatttcc
     5041 tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt ccagcgcttg
     5101 atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat gctgccaaca
     5161 atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa aaggcaaatc
     5221 acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg gttaacggaa
     5281 aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg catgtgtacg
     5341 cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg gaacaggtcc
     5401 gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc tttggcttgc
     5461 aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc aggcatgacc
     5521 agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg gacaaagaat
     5581 tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat gtcgaccttg
     5641 tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg cttgcttgcg
     5701 ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag cgaaagttta
     5761 aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc cggccaacat
     5821 gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg tcagtcatcc
     5881 gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat acggtgatag
     5941 ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat agcgcagccc
     6001 ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc atggaattcg
     6061 acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc acgcacacca
     6121 ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc ggtcgtggtg
     6181 tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt ttgcagcccc
     6241 agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa aatgctgatg
     6301 cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct ggaaaccatc
     6361 aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa cgccatgcct
     6421 tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg caaggtttcg
     6481 caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat ggcggagaca
     6541 atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa atccggacgc
     6601 gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt tacgccttcc
     6661 gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga acggatggta
     6721 cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc gtgcatacca
     6781 aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc gtagtcgggc
     6841 ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg ctgctggcct
     6901 ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat gggcaaaaca
     6961 tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga aaatagcatg
     7021 agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct ggatcccgag
     7081 cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa tattctggca
     7141 gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat ggatgactgg
     7201 aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag cacgatgggc
     7261 gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc tgcgggaaaa
     7321 tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa atacatgccg
     7381 gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa agagctggcg
     7441 cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca tcaggcgatg
     7501 gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt tatcaagctg
     7561 taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg attttgacgc
     7621 taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa aaaaactttt
     7681 ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac gacgcagaaa
     7741 tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt ggtagagcac
     7801 gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccaa aatttgaaaa
     7861 gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa ttgcgacaag gcggcaactg
     7921 aacgaatcct tgggaactta caaaagtatg tgactaaggt gagtgaaggc agccaaggca
     7981 gtagcggttc gaaagacgaa gat
//
LOCUS       DAJVQR010000151         7899 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVQR010000151 DAJVQR010000000
VERSION     DAJVQR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(123..953)
                     /locus_tag="OQM52_003756"
     CDS             complement(123..953)
                     /locus_tag="OQM52_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1565432.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OQM52_003757"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OQM52_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1565433.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OQM52_003758"
     CDS             2379..2729
                     /locus_tag="OQM52_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCS1565434.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OQM52_003759"
     CDS             2855..3415
                     /locus_tag="OQM52_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1565435.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OQM52_003760"
     CDS             3418..6384
                     /locus_tag="OQM52_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCS1565436.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OQM52_003761"
     CDS             6451..6828
                     /locus_tag="OQM52_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1565437.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OQM52_003762"
     CDS             complement(7029..7688)
                     /locus_tag="OQM52_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1565438.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1750 a         2239 c         2130 g         1780 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcg
//
LOCUS       DAJVQR010000152         7726 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVQR010000152 DAJVQR010000000
VERSION     DAJVQR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OQM52_003763"
                     /pseudo
     CDS             complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OQM52_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            763..915
                     /locus_tag="OQM52_003764"
                     /pseudo
     CDS             763..915
                     /locus_tag="OQM52_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2296..3243
                     /gene="icsP"
                     /locus_tag="OQM52_003765"
     CDS             2296..3243
                     /gene="icsP"
                     /locus_tag="OQM52_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCS1565439.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3805..4177)
                     /locus_tag="OQM52_003766"
                     /pseudo
     CDS             complement(3805..4177)
                     /locus_tag="OQM52_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4421..4540
                     /locus_tag="OQM52_003767"
                     /pseudo
     CDS             4421..4540
                     /locus_tag="OQM52_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4790..5656
                     /gene="ospB"
                     /locus_tag="OQM52_003768"
     CDS             4790..5656
                     /gene="ospB"
                     /locus_tag="OQM52_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCS1565440.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5985..6725
                     /gene="phoN2"
                     /locus_tag="OQM52_003769"
     CDS             5985..6725
                     /gene="phoN2"
                     /locus_tag="OQM52_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCS1565441.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7085..>7726)
                     /gene="ospC4"
                     /locus_tag="OQM52_003770"
     CDS             complement(7085..>7726)
                     /gene="ospC4"
                     /locus_tag="OQM52_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCS1565442.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYK
                     VLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2419 a         1449 c         1448 g         2410 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaatctctg gcgactcatc tcttaacact
      181 tgcataatct ctctttttat atcatatagt tctataaacc gatttgcccg tagttctcct
      241 tgggaagaat catttctgaa tacaacaatt atatacaaaa aatcactatt ttctttagag
      301 aagatcttac aactaatctc tgggagctca cataatgcat gtaagtcagg atggtacaaa
      361 ctatccacag aaaattgccc cataggacgt tttccagata taactgtcaa ccagcttcct
      421 tccggtgtac tggcttcggc tctggaaata ggctgaaaat cttccggaca tggagaatac
      481 attgtcttca tataattaag tcgtctggat ttctcattaa taaactcctc tatttttaat
      541 tgtgccatac ttctggttat accagagata tacttcttgt catatatgtt tggtgattca
      601 ttaaagtaac catgttcaac tctaggataa atatttctca ctatatattc agtatttgca
      661 gtatttattg gaaatacgtt cttaatattc ttgatgggac taatcatttt aatctctata
      721 ctcaaatata aacattacca tgaacaatat aattgttcct aagtggaatg tctccacgga
      781 gaacacttta tctatcggga aatagtgcgg gcaacagtgt ttaattatat cgagtgcaat
      841 tacaatcgct ggcgacggca ccgttggtgt ggcggcctca gtccggaaca atttgaaaac
      901 cagaacctcg cttaggcctg tgtccatatt acgtgggtag gctcacagag cctcacttac
      961 gactgcgtcc atattatgtg ggtaggatca ataaagacgt tttatatgtg tctgcttgca
     1021 gccagaaaat ttataccctg caggtatcat aaacatgctt gataacttaa ttggggctcc
     1081 ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg
     1141 aagtatattt aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagcttat
     1201 ccaaactgcc aagcctcttt attataagta agatctggca ctggaatatt ctcaaaagga
     1261 ctaacatcat caccacgacc ttcaaccaac cattttattg catttaaatc ccagaatagt
     1321 tcagtagtgg tcaatttggg aaacgcacaa tattgtacgg ttacgttatc gcaatagagt
     1381 gaactctcgc cctatacggc gttttcaagg attagtcctt aatcggacaa ccacattaaa
     1441 gcataataaa taattaagct ttaataatat actatagata cagttctatt ataaaaaata
     1501 ctattttagg tgatgcatta aattagtaga atatttattc ttttataaaa gcaccaatga
     1561 attgtccaag taaacatgag ataaaatgtg tagaggataa ctgagtttgg tcactttaac
     1621 tgtattagtc gcagtctgac tctcgacttt aaaaggatgg gaattacctg tactgatagt
     1681 gagaaagctt tccattgaaa taagtcaaat aaaagttgat ggcttcagtg ctaaagtgag
     1741 gtgcccgcta gccgtattca ctcgaatcgg aattcaatgt attgcttctg ccatttccat
     1801 aatatttcac gggaaaaaca aaatctgaaa aactatactt aagtgttgta ttgagtcagt
     1861 tgagatacaa atacatattt atcagcacac gttacgtcaa atacaggagg ttgtcatgtt
     1921 ggtactgaaa ggcacgttgg ctattgcagc cgtgatgaca atttattgtc ttatcgttgt
     1981 tcttatggaa cgcttttctg attgatttca tattggcgag agaacaagaa ggtgagtaga
     2041 attgtcgcgg aatcctgaaa ctatcagcct caggtatgaa caccaaaagg taggtagaga
     2101 aatctatggc ccccgttact ttattcgcac aatgaggtat ttttttattt ttacaaaagt
     2161 tcaataaata ttataataaa agatatgttc ttggtaacat aaacaaatat ttaattaaaa
     2221 taatgcttgg cattgacgtt tatcttataa agtaagaaga tcatggacat ttcaaccaaa
     2281 aaagtagagt tctcgatgaa attaaaattc tttgtacttg cactttgtgt acctgcgatc
     2341 tttactacac atgctaccac taactatcca cttttcatac cggacaacat cagtacggat
     2401 attagtctag gatctctgag tggcaaaaca aaagaacgcg tttatcatcc caaggaaggg
     2461 gggagaaaaa ttagtcaact ggactggaaa tacagtaatg cgactattgt tagaggtggc
     2521 atcgattgga agctaattcc aaaagtgtct ttcggagttt ccggttggac tactttaggt
     2581 aaccagaaag caagcatggt tgataaagac tggaacaatt ccaatacccc tcaggtatgg
     2641 accgaccaga gctggcatcc caatacgcat ctccgtgatg ctaacgaatt cgagctgaat
     2701 cttaaaggtt ggttattaaa taatttggat tatcgacttg gactaatagc aggttaccaa
     2761 gagagtcgtt acagttttaa tgcaatggga gggagttata tttatagtga aaacgggggg
     2821 agcaggaata aaaaaggggc acatcctagt ggtgaaagaa caataggtta caaacagctc
     2881 ttcaaaatac cttatattgg attaactgct aattaccgcc atgagaattt tgagtttgga
     2941 gcagaactga aatatagtgg ttgggttctt tcatctgata cagataaaca ctatcagact
     3001 gagacaattt ttaaagatga aataaaaaac caaaattact gctctgttgc tgcgaatatt
     3061 ggatactatg tcacccccag tgcaaaattt tatatagaag gctccagaaa ttacatttct
     3121 aataaaaaag gtgatacatc tctttatgag caaagtacca atatatctgg caccattaaa
     3181 aatagtgcaa gtattgaata tattggtttt ctcacttccg caggtataaa gtatattttt
     3241 tgaattggtt caccgagttg attacgtttt catggctaaa aattgccgcc cattgtgagt
     3301 catattcgcc ctgactttcc agaaccatac gaactgcccg ttgacggact tcgggggaaa
     3361 aacgtgtatt tttagtcatt ctgtttacct ctttctcagg gcgtttagtc tccaggattt
     3421 ccggggcggt tcaaaaaact cttcgttgat tactgtggac ttactgttgg cattaccgac
     3481 cctgagaccg gagaaataag aaccgcttag atcatcgtgg ctgttctcgg ggtatcaagt
     3541 tacacatggg cagaggctac ccaaggtcta ctattatcgc ctgaaagcgc tgatggaaca
     3601 gtgctatcag ggacatgctg atgaaaatac ctaaataatc agccagcact ctatctttcc
     3661 aaatccacag catagcaaag agagcaaaag agcctgtaaa ttcagaaatt aaggaaacgc
     3721 ctcttggtgt acataatcta tcttatgtta aatgaaatct aatttcttga ttatatgctg
     3781 gatcgttcgt gggtggcttt gcccctatat ttccagacac ctgttatcat tggcccgctg
     3841 ccgcagatat tcccgtggcg agcgataatc cagcgcacta tgcggatgcc attcgttata
     3901 atgctcgaat gcctctgcaa ggttctttgc tgccgttaac ccgtcaggtt tgggcatgat
     3961 actgatgtag tcacacttta ttgttttcac gaagctcttt gctattctgt tactctccgg
     4021 actccgcacc gccgtgttct tcggttcaac aaagtcccaa catccgggtg aactggcgtg
     4081 tttcattagc tcggtagcat gaaccattat ccgtcagtca cttcactgga gacgccggaa
     4141 gctcgttgcc gaagcggtgt tccaccgctc ccagcatgtc gccaacggga gagcatctac
     4201 ggtctgattg aactgtttat tcaaattgtc caccgtctgt cagtacgctc tgaacgtcgg
     4261 ctggttaaaa cactgctcgc tgattttcag agggttcacg gtaaaacagc acttttattc
     4321 cggattgcgg aagcggctct gaataatccg gatgggctgg ttaaagaggt tgtgtaccat
     4381 ctgcatattg ttgaaccaga tcgcagcggt aagagaagtt catcatactt ggcgcagtta
     4441 cgcgatgttt ctgctagagg tgatgccgta aagaacggca gaacattacc ggaacaagat
     4501 agcggtctac ctgcactggt atcagacccg gggcttcccc ggatgatctc cacggtactt
     4561 taggtactgt gtctatatta tgtaggtagg atcaattaac tttgtagttg aaattctttc
     4621 attgaaataa tgaataaaaa agacaaaccg cattaaacaa tgccagaata taattgcatt
     4681 tttgttccgt ttttttaaat catagtatac aaagaagctt gcatactata tgaaaatata
     4741 acacccttta ctatgatgtt tataaaacaa tatatggagt catgtaggta tgaatttaga
     4801 tggtgttaga ccatactgta gaatagtcaa taaaaagaat gaaagcatat cagatattgc
     4861 atttgcacat ataataaaaa gggtaaaaaa ttcatcatgt actcacccaa aagcagcatt
     4921 ggttttttta ggagagaaag gtttttgtga tagcaatgat gttctatcta ttatgggaca
     4981 acaaatacca agagtattta agaacaagat gttatatgat tatgttttta aaaatgaaaa
     5041 aagtaaaaat gattttctaa aaatggctga atcatggcta ccacagagtg aaccaatagt
     5101 aataaataat gatgatgacg cattgaatgc tgctgcttat ttttctgtaa aaaaagcgaa
     5161 aataaaaaca gtaaacgata ctgattttaa agagtataat aaggtttata ttcttgggca
     5221 cggtagtcct ggttctcatc aattaggcct tggttcggaa cttattgatg tacaaacaat
     5281 catttcaaga atgaaagact gtggtattct aaatgtgaaa gatatccgtt ttacttcatg
     5341 cggctccgct gataaagtgg ctcctaaaaa ttttaacaat gcccctgctg aaagtctttc
     5401 ttgtatcctt aactctctgc ctttttttaa ggaaaaagaa tctttgctag agcagataaa
     5461 aaaacacctt gaaaacgatg agtcattgag tgatggtcta aaaatatccg gctatcatgg
     5521 atatggagtt cactatggtc aagagctttt tccctactca cattatcgtt caacttcaat
     5581 tcctgctgat ccggagcata cagtaaaaag aagctctcag aaaaagactt ttattattaa
     5641 taaagaactg gattagtata aaatttttaa cctatagtta attgtttgca tttaagatga
     5701 agttatttta tctttagtag aaaagtgctt ccctatgagg gaaaatcaca aatcatcata
     5761 atacagagac aaaacgatac gaaaaaatag ataaaaaaca tcattctttt accacattat
     5821 tttccgtgaa tatgaaaaat aatgttatta ctttaatata agactatttt ttgtttttcc
     5881 atcactctgt tcaaattttt ccgcatgact tgtgtttttt gtaatacagc tcgtttttta
     5941 cagctgacca aaatcatcaa ttaattatgc taaggaaata aattatgaaa accaaaaact
     6001 ttcttctttt ttgtattgct acaaatatga tttttatccc ctcagcaaat gctctgaagg
     6061 cagaaggttt tctcactcaa caaacttcac cagacagttt gtcaatactt ccgccgcctc
     6121 cggcagagaa ttcagtagta tttcaggctg acaaagctca ttatgaattc ggccgctcgc
     6181 tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc atactacgag aattttggtc
     6241 ttgcattttc agatgcttat ggcatggata tttcaaggga aaatacccca atcttatatc
     6301 agttgttaac acaagtacta caggatagcc atgattacgc cgtgcgtaac gccaaagaat
     6361 attataaaag agttcgtcca ttcgttattt ataaagacgc aacctgtaca cctgataaag
     6421 atgagaaaat ggctatcact ggctcttatc cctctggtca tgcatccttt ggttgggcag
     6481 tagcactgat acttgcggag attaatcctc aacgtaaagc ggaaatactt cgacgtggat
     6541 atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg gcaaagcgat gtagaggctg
     6601 ggcgtttaat gggagcatcg gttgttgcag tacttcataa tacacctgaa tttaccaaaa
     6661 gccttagcga agccaaaaaa gagtttgaag aattaaatac tcctaccaat gaactgaccc
     6721 cataaagctg gacagcctgt atcaggctat ggagggccca tagacaaatc taccctatat
     6781 gagcatagga ggagtctatg ggcacaccac gttttacccc tgaatttaag ggattactgg
     6841 aaaggctggg acatatccct ccggcagaag cagaaaaagc ttattatgct gccatcggaa
     6901 acgatgatct ggcaacctga gttcacagat aaaacattct ctaggaaact cggggcggtt
     6961 ccgttcacca catgcaatgt ggtgttgcag gggaacggtc tgcaccatcc cctatgtcga
     7021 tcaatataac agaaatgaca acttcagatt cagggcacaa cctaaatata ttttaggtca
     7081 cctctcaaat cgtttgcctg ataccgctcc attttttaat aaaaaaatca atcatttttg
     7141 aatctttgct tttcattgca ttgtcaagca tagtgtcccc actatttgct ttttgaaatt
     7201 ttttgtttac atcaaccaat ccattattga taaaatattc tagcacttta tagttggcac
     7261 catctttact aagtaagtat tcaacatcat acagattaca atatctattg tttttcattt
     7321 tctccatctc tgcgacatct tgttttgtaa aattaaattt agatagtaaa taaagtgcta
     7381 tatcagcttt tttattgcct aaagaataag caagggcctg atgagccatt tttttatcag
     7441 tcactttatt gttaatctct tcataattag atgctttaat cgcatcctcc aaagatatgt
     7501 ctctgagttt tatttttttg aagttatctg tacttagcat tcttggtata tgatactcaa
     7561 gctgaaacac aaagtttata attctatcca gactaacagg atcaatattt ttgttataac
     7621 aaaactctct gaatccttga tctttactct ttcttataaa atcaataaga tgcaacccaa
     7681 ttccaagcct catatcttta gtgttaaata ttggaacatc agtttt
//
LOCUS       DAJVQR010000153         7723 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVQR010000153 DAJVQR010000000
VERSION     DAJVQR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..832)
                     /locus_tag="OQM52_003771"
     CDS             complement(<1..832)
                     /locus_tag="OQM52_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCS1565443.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="OQM52_003772"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="OQM52_003772"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1565444.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGTWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /locus_tag="OQM52_003773"
     CDS             complement(2481..2708)
                     /locus_tag="OQM52_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1565445.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="OQM52_003774"
     CDS             complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="OQM52_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1565446.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="OQM52_003775"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="OQM52_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1565447.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="OQM52_003776"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="OQM52_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1565448.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="OQM52_003777"
     CDS             complement(5530..6513)
                     /locus_tag="OQM52_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1565449.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="OQM52_003778"
     CDS             complement(6559..7383)
                     /locus_tag="OQM52_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1565450.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="OQM52_003779"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="OQM52_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS1565451.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2069 a         2013 c         1811 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg gacatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac tca
//
LOCUS       DAJVQR010000154         7542 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVQR010000154 DAJVQR010000000
VERSION     DAJVQR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="OQM52_003780"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="OQM52_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="OQM52_003781"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="OQM52_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1565452.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="OQM52_003782"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="OQM52_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="OQM52_003783"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="OQM52_003783"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1565453.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDKIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="OQM52_003784"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="OQM52_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1565454.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="OQM52_003785"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="OQM52_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1565455.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="OQM52_003786"
     CDS             complement(6512..7204)
                     /locus_tag="OQM52_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1565456.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1919 a         1829 c         1987 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aaaatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac ccccccccgc gccgctgaga aaaagcgaag cg
//
LOCUS       DAJVQR010000155         7398 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVQR010000155 DAJVQR010000000
VERSION     DAJVQR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..1014
                     /gene="ydfJ"
                     /locus_tag="OQM52_003787"
     CDS             <1..1014
                     /gene="ydfJ"
                     /locus_tag="OQM52_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCS1565457.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1103..2563
                     /locus_tag="OQM52_003788"
     CDS             1103..2563
                     /locus_tag="OQM52_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1565458.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="OQM52_003789"
     CDS             complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="OQM52_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1565459.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2980..3666)
                     /gene="ydfH"
                     /locus_tag="OQM52_003790"
     CDS             complement(2980..3666)
                     /gene="ydfH"
                     /locus_tag="OQM52_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS1565460.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="OQM52_003791"
     CDS             complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="OQM52_003791"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1565461.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4638..6683
                     /gene="dcp"
                     /locus_tag="OQM52_003792"
     CDS             4638..6683
                     /gene="dcp"
                     /locus_tag="OQM52_003792"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1565462.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6728..7246)
                     /locus_tag="OQM52_003793"
     CDS             complement(6728..7246)
                     /locus_tag="OQM52_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS1565463.1"
                     /translation="MNINKDKIVQLADTDTIENLTPALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1830 a         1745 c         1884 g         1939 t
ORIGIN      
        1 atcttgctgg tgacgctgcg tattattcag ggattgggtg caggtgcgga aatttccggt
       61 gccggtacga tgctggcgga atatgcgcca aaaggtaagc gcggaattat ctcctcattt
      121 gtggctatgg gaactaactg cggcacctta agcgcaacgg caatctgggc ctttatgttc
      181 ttcattctca gtaaagagga actgctggcg tggggatggc gtataccgtt cctggcgagc
      241 gttgtcgtga tggtctttgc tatctggttg cgtatgaatc tgaaagaaag cccggtcttt
      301 gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg cacctgctgg tagcatgttc
      361 cagagcaaat ccttctggct ggcaacaggg ctgcgttttg gtcaggcggg taactccggt
      421 ttaattcaga ctttccttgc aggctattta gtacagacgt tattgtttaa caaagcaatt
      481 ccaacagatg cattgatgat cagttcgatt ctcggcttta tgaccattcc gttccttggt
      541 tggttatccg ataaaattgg tcgccggatc ccgtatatta ttatgaatac ctctgcgatt
      601 gtgctggcgt ggccaatgct ttctatcatc gtagataaaa gctatgcccc gagcaccatt
      661 atggttgcac tgattgtgat tcataactgt gcggtgctgg gattatttgc tctggaaaac
      721 attaccatgg cagaaatgtt cggctgtaaa aaccgcttca cccggatggc tatttcaaaa
      781 gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc tggcgggtat tttctgcacc
      841 atgacggaat cctggtatcc gatcgccatt atgatcatgg catattcagt gattggttta
      901 atctctgcgc tgaaaatgcc agaggtgaaa gaccgtgatt taagtgcgct ggaagacgct
      961 gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt ccagaagtct gtaaaccctc
     1021 aatcccttct cttaccggag aggggatttt tattcatata acaaaacata tagcttgcca
     1081 tatttaaatt taaggaattg ttatgggaaa taatttgtta tcagcaaaag cgacactccc
     1141 tgtttatgat cgtaataacc tggccccaag aattgttcat ttaggctttg gtgcatttca
     1201 ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaacatttca gtgactgggg
     1261 atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac aacagcaaga
     1321 taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggctcgcg tcgttggcgt
     1381 cgttaaaaaa gccttgcacg tacagatgga tggcttagaa accgtgttgg ctgcgatgtg
     1441 tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt tccactctcc
     1501 ggcgaccgga cagttaatgc tcgagcaccc gatggtcgct gccgacgtgc aaaatcccca
     1561 ccagccgaaa acagcaacag gggtgattgt tgaggcgctg gctcgccgta aagcggcagg
     1621 acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc atgttatgcg
     1681 tgacgttgtc acttcctacg cgcaagccgt tgatgtaaaa ctggcacaat ggatcgaaga
     1741 taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga cagaggatac
     1801 gctggcgaaa atcgaacaac ttaccggtgt gcgcgatcct gctggcgttg cctgtgaacc
     1861 tttccgccag tgggttatcg aagataactt tgtagccgga cgtccggaat gggaaaaagc
     1921 gggagccgaa ctggttagtg atgtgctgcc ttatgaagag atgaagttgc gcatgctcaa
     1981 cggcagtcat tcattcctgg catatctggg ttatcttgct ggatatcagc acattaatga
     2041 ctgtatggaa gatgaacatt atcgtcatgc ggcgtatggc atgatgttgc aggaacaagc
     2101 gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat taattgcacg
     2161 ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg gcagccagaa
     2221 attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca gcaagttcga
     2281 tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg atgaacaggg
     2341 aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg tacaaagtag
     2401 tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcaatct ttggtgatga
     2461 tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt ctttattagc
     2521 gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag taaaaaaagc aatgccgcca
     2581 tcggcggcat tcacacactt agttcatgcc gagacgaatc aggtccagag gtgccagttt
     2641 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
     2701 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
     2761 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
     2821 gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg agtgaaatat
     2881 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccgt aaggcggggg
     2941 gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat tactcttcgc taaaccagtc
     3001 gctgttttcc tggcggatct ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat
     3061 tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat caagatgctg
     3121 gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca cgcgatcaac
     3181 ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa tctgcgtcag
     3241 gagttgatgg aagttgtcat caagttcaaa aaaatcatcc agttgcttgc gctcaatggc
     3301 aatgcgttgc tggtgaagat tttgttccag ttggtagcac tggctttcgg taatcatgct
     3361 cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac
     3421 ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt gaatcaggcc
     3481 gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat tgaaacgaac
     3541 agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg
     3601 acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt gcgtttcgac
     3661 ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt ttttgtgaca
     3721 agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca ttcagtccgg
     3781 catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga gcaggcagcg
     3841 ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg gtattttgat
     3901 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
     3961 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
     4021 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
     4081 acggccagct gcctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
     4141 tacccggtaa gacggcgcgc gtcatatata ctaggccttt gttgttggta tcaatcatcg
     4201 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aggccggcat
     4261 tatttaccag gatatcaata ttgctccact cggcagggag cgatgccagc atctcttcaa
     4321 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctg
     4381 ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc ccttgttgaa
     4441 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
     4501 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
     4561 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
     4621 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
     4681 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
     4741 gaatgcaaca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
     4801 tcaacaatac tattctggca ctggaacaaa gcggagaatt aattacccgc gttaccagcg
     4861 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     4921 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     4981 gggtagatgc tgtctggcag cgccgtgaat ccctggggct tgatagtgaa tccatccgcc
     5041 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgca caagctgata
     5101 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     5161 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     5221 gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata
     5281 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     5341 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     5401 ccaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     5461 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacgccag
     5521 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg
     5581 aattagcctc catacaggcg gttatcgata agcagcaggg cgggtttagc gcgcagccgt
     5641 gggactgggc attttatgcg gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     5701 agctcaagcc atattttgaa ttaaacacgg tgttaaatga aggtgtattc tggaccgcga
     5761 atcagctctt cggtattaag tttgtcgaac gttttgatat tccggtctac catcctgacg
     5821 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     5881 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     5941 cgcttaatga aacgcatccg gtaatttata acgtctgcaa ttatcagaaa cccgctgccg
     6001 gtgagcctgc gttgttactc tgggatgatg taataacctt attccatgaa tttggtcata
     6061 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     6121 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     6181 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     6241 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     6301 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     6361 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     6421 atcgcagcag ttatttcgcc catatttttg gtggcggata tgctgcaggt tattacgctt
     6481 atctgtggac gcaaatgttg gccgatgatg gttaccagtg gtttgttgag cagggcggat
     6541 taacgcgtga aaatgggcag cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     6601 atctggaacg cctgcatcga caatggcgtg gtaaggctcc tcagattatg ccgatgctgc
     6661 aacatcgtgg cttgaacata taaacaccac caatggctgg cctggagata tcgccaggcc
     6721 agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc gctaaagcta
     6781 ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt gtagttctgg cctttaatat
     6841 gccacgcgaa ccagacggta cccgctggcg taccatcttc accgccctcc ggtccgccgt
     6901 agccggtaat ggcaatactg acatcagcat ccgcacgctc tatggcacct gttgccattt
     6961 ctgctgccac tttctcactc accgcagaat atcgttcaag agactgctgg cttacgctga
     7021 ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa aatttgggtg
     7081 tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat tcggcggtag
     7141 ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc gggtgtcagg ttttcaatag
     7201 tgtcggtatc tgcgagttgt acaattttat ctttattaat gttcactgtt ttttgtccac
     7261 gctattatat gtaaaggtga tttgcttttt ctgtatgctc gtccaatata ataattttca
     7321 aacatataaa tccgattatt ctgaaaacat gtttaagact cttcaatctt cttaacttta
     7381 gtgagtcaaa ttctctga
//
LOCUS       DAJVQR010000156         7202 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVQR010000156 DAJVQR010000000
VERSION     DAJVQR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            26..1516
                     /locus_tag="OQM52_003794"
     CDS             26..1516
                     /locus_tag="OQM52_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1565464.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1568..2725
                     /locus_tag="OQM52_003795"
     CDS             1568..2725
                     /locus_tag="OQM52_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1565465.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2691..2813)
                     /locus_tag="OQM52_003796"
     CDS             complement(2691..2813)
                     /locus_tag="OQM52_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565466.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3016..4701
                     /locus_tag="OQM52_003797"
                     /pseudo
     CDS             3016..4701
                     /locus_tag="OQM52_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4739..7099
                     /locus_tag="OQM52_003798"
     CDS             4739..7099
                     /locus_tag="OQM52_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1565467.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         2115 a         1572 c         1527 g         1988 t
ORIGIN      
        1 aaagccaaat attatcgtac taaccatgga tgatcttggt tatggacaac ttccttttga
       61 taagggatct tttgacccaa aaacaatgga aaatcgtgaa gttgtcgata cctacaaaat
      121 agggatagat aaagccattg aagctgcaca aaaatcaacg ccgacgctcc tttcattaat
      181 ggatgaaggc gtacgtttta ctaacggcta tgtggcacac ggtgtttccg gcccctcccg
      241 tgccgcaata atgaccggtc gagctcccgc ccgctttggt gtctattcca ataccgatgc
      301 tcaggatggt attccgctaa cagaaacttt cttgcctgaa ttattccaga atcatggtta
      361 ttacactgca gcagtaggta aatggcactt gtcaaaaatc agtaatgtgc cggtaccgga
      421 agataaacaa acgcgtgact atcatgacaa cttcaccaca ttttctgcgg aagaatggca
      481 acctcaaaac cgtggctttg attactttat gggattccac gctgcaggaa cggcatatta
      541 caactcccct tcactgttca aaaatcgtga acgtgtcccc gcaaaaggtt atatcagcga
      601 tcagttaacc gatgaggcaa ttggcgttgt tgatcgtgcc aaaacacttg accagccttt
      661 tatgctttac ctggcttata atgctccgca cctaccaaat gataatcctg caccggatca
      721 atatcagaag caatttaata ccggtagtca aacagcagat aactactacg cttccgttta
      781 ttctgttgat cagggtgtaa aacgcattct cgaacaactg aagaaaaacg gacagtatga
      841 caatacaatt attctcttta cctccgataa tggtgcggtt atcgatggtc ctctgccgct
      901 gaacggggcg caaaaaggct ataagagtca gacctatcct ggcggtactc acaccccaat
      961 gtttatgtgg tggaaaggaa aacttcaacc cggtaattat gacaagctga tttccgcaat
     1021 ggatttctac ccaacagctc ttgatgcagc cgatatcagc attccaaaag accttaagct
     1081 ggatggtgtt cccttgctgc cctggttgca agataagaaa caaggcgagc cacataaaaa
     1141 tctgacctgg ataacctctt attctcactg gtttgatgag gaaaatattc cattctggga
     1201 taattaccac aaatttgttc gccatcagtc agacgattac ccgcataacc ccaacactga
     1261 ggacttaagc caattctctt atacggtgag aaataacgat tattcgcttg tctatacagt
     1321 agaaaacaat cagttaggtc tctacaaact gacggatcta cagcaaaaag ataaccttgc
     1381 cgccgccaat ccgcaggtcg ttaaagagat gcaaggcgtg gtaagagagt ttatcgacag
     1441 cagccagcca ccgcttagcg aggtaaatca ggagaagttt aacaatatca agaaagcact
     1501 aagcgaagcg aaataactaa accttcatgc ggcggatttt tccgccgcct tattgagcga
     1561 gatagcgatg cacgttacag ccaagccctc cagttttcaa tgtaatctca aatgtgatta
     1621 ctgtttttac cttgaaaaag agtcgcagtt tactcatgaa aaatggatgg atgacagcac
     1681 tctgaaagag ttcatcaaac aatatatcga agcgtctggc aatcaggtct attttacctg
     1741 gcaaggcggt gaacccactc tggctggcct ggattttttc cgtaaagtta ttcactatca
     1801 acaacgctat gcaggccaaa aacgtatttt taatacatta caaacgaatg gcattttatt
     1861 gaataatgaa tggtgtgcct ttctcaaaga acatgaattt ctggttggta tctcgatcga
     1921 tggcccccag gagttacatg accgttacag acgcagtaat tcaggtaacg gtacttttgc
     1981 aaaagtgata gcagccatcg agcgtctgaa atcatatcaa gtagagttta atacgttaac
     2041 cgtcattaat aacgttaatg tccattatcc tcttgaggtt tatcattttt taaaatctat
     2101 cggcagtaaa catatgcaat ttatcgaatt gctagaaacc gggacgccga atattgattt
     2161 cagtggtcat agtgagaaca cattccgtat cattgatttt tctgtgcctc ccacggctta
     2221 tggcaagttt atgtcaacca tttttatgca atgggttaaa aacgatgtgg gtgaaatttt
     2281 cattcgtcag tttgaaagct ttgtcagccg ttttttgggg aatgggcata caagttgtat
     2341 tttccaggag tcctgcaagg ataatctggt tgttgaaagt aatggagaca tttacgaatg
     2401 cgaccatttt gtctatccac agtacaaaat tggaaacatt aataaatctg aactcaaaac
     2461 gatgaacagt gtacaactga cagcgcaaaa aaaacggatt tcagcgaaat gtcagcaatg
     2521 tgcatataaa cctatctgca atggcggttg tcctaagcat cgtattacta aagtaaacaa
     2581 tgagactgtt tcttattttt gcgaaggtta taaaatcctt ttttcaacca tggtacctta
     2641 tatgaacgcc atggttgagt tagctaagaa cagagtaccg ctttaccaca ttatggatgt
     2701 tgcaaaacaa atggagaata attaataacg ctctctttta actccattct tagcctcctt
     2761 cttatgacct taacaaggca cccgacataa attcctgtcg ggtgtttcgt catcatcatc
     2821 ttcccttgcg aaaatcctgg cttacgtggt atgcgtcagg gcattagcaa tacccttgat
     2881 tatcaatagc ataaccataa ccactgaaaa ttaaaaattt ctatatattt catattgtta
     2941 aatgatgctt aaataaattt catcagtaaa tcattgccat agataatcat tggatggcac
     3001 aatcaatttt cgctaatgat aaccattccc attacgtttt gtatgttgat agcaaagtat
     3061 ctctgtctgc tgaagccctt ttggctgcgt aagaataata aaacttcagt gttgttaatc
     3121 attattatcc tcgcaatgat ccttggcgtg gttaaaattc aggtctggtt aaacgactgg
     3181 aataatgatt ttttcaatgc gctaagccag aaagaaaccg ataagctttg gcagctcgtt
     3241 ctttggttcc ctgccctgct ggggatcttt gtattaatat ccgcaaacaa aacatggtta
     3301 attaaactac tgactattcg ctggcgcgaa tggttaacag attactacct taatcgctgg
     3361 tttgcagata aaaactacta cttcacgcag atctacggcg agcataaaaa tacagataac
     3421 cctgaccaac gtatcgctga agacattctc ttactgatca acaaaacgct cagtctttca
     3481 tttggcttca tccagtcact tagcatgctg atcaccttta ccgttattct ttggcaaagc
     3541 gcgggtacgc tctcattcac tgttggtgga acagaatgga atatccaggg gtatatggtc
     3601 tataccgtcg tgctcattgt gatcggtgga actttattta cccataaggt gggtaaacgt
     3661 attcgtccgc ttaatgtgga aaaacaacgt agcgaagcga catttcgaac taatcttgtg
     3721 cagcataata agcaggcaga actgattgcg ctaagcaatg ctgagtctct acaacgccag
     3781 gagttgcgcg acaattttca taccattaaa gagaactggc atcgtttaat gaatcgtcag
     3841 cggtggcttg attactggca aaatatttat tcgcgctcgc ttagcgttct tccctacttt
     3901 ctgttattgc cgcagtttat tagcgggcag atcaatctgg gcggactgat gaaatcgcgc
     3961 caggcattta tgctagtatc gaacaattta agctggttta tttataaata tgacgaactt
     4021 gctgaactgg ctgcggttat cgatcgcttg tatgagttcc atcaactcac tgaatagcgc
     4081 cctacgaata agcctaaaaa ttgccaacat gcggtacaag tggctaatgc gagtattcgt
     4141 acgcctgata ataagatcat attagagaac ttgaactttc atgtttcgcc aggcaaatgg
     4201 ctattactga aaggctactc tggtgcggga aaaaccacac tgcttaaaac attatcccac
     4261 tgctggccgt ggtttaaagg tgatatttct tctcctgctg acagttggta tgtgtcacaa
     4321 acaccgttaa tcaaaagcgg cttactgaaa gagattattt gtaaagcact tcccctgccc
     4381 gtagacgata aatcgttgag cgaagtactg catcaggttg gtctgggaaa attggctgcg
     4441 cgtattcatg accacgatcg ctggggagat attctttcca gcggcgaaaa acaacgtatc
     4501 gccctggcac gattaatttt acgacgtccg aaatggatat ttcttgacga aactacctct
     4561 catcttgagg aacaagaggc tatacgctta ctgcgtttag tgcgtgaaaa actacccaca
     4621 agcggcgtca ttatggttac acatcaaccc ggtgtctgga acctggctga tgatatttgt
     4681 gacattagca cggttttata acaacaataa aagccctgct gtacggagat ataaataaat
     4741 gaagcgagtt cttattcctg gcgtcatttt atgtggcgct gatgtggcgc aggccgtcga
     4801 tgacaaaaac atgtacatgt atttttttga agagatgacg gtctatgctc ctgtccctgt
     4861 acccgtaaac ggcaacacgc attacaccag tgaaagcatc gagcgtttac cgaccgggaa
     4921 tggcaatatc agcgatctgc tgagaaccaa ccctgcggta cgcatggatt caacgcaaag
     4981 tacctcgttg aaccagggag atattcgccc tgagaaaatc tctattcacg gtgcgtcgcc
     5041 ctaccagaat gcctatttga ttgacggtat tagtgcaact aataacctga acccagcgaa
     5101 tgagtccgat gccagtagtg caaccaatat tagcgggatg tcacaggggt attatcttga
     5161 tgtcagctta ctggacaatg tgacgcttta tgacagtttt gtgccggttg aatttggtcg
     5221 cttcaatggc ggggtaattg atgcaaagat caaacgcttc aacgctgatg atagcaaggt
     5281 gaaattgggt tatcgcacta cgcgtttgga ctggttaaca tcgcatatcg atgagaataa
     5341 caagagcgca tttaatcaag gttcttcagg aagtacctat ttctctccag attttaaaaa
     5401 gaacttttat accttgtcgt ttaatcagga gctggctgat aactttggcg ttaccgccgg
     5461 tttatcgcgc cgccagtctg atatcacccg cgcggattat gtttcgaatg acggcattgt
     5521 cgccggtcgg gcacagtata aaaacgttat cgatactgca ttgagcaaat ttacctggtt
     5581 tgccagcgac cgctttaccc acgatttaac cttaaaatat accggctcca gccgtgatta
     5641 taataccagc accttcccgc agtctgatcg cgaaatgggt aataaatcct atggtctggc
     5701 atgggatatg gatacgcaac tcgcatgggc caaactacgt accaccgttg gttgggatca
     5761 tattagtgat tatacccgtc acgatcatga catctggtac accgaacttt catgtacata
     5821 tggtgatatt acagggcgtt gtacccgtgg cggattagga cacatttccc aggctgtaga
     5881 taattacacc ttcaaaacac gcctggactg gcaaaaattc gccgtgggtg atgtttcgca
     5941 tcaaccctac ttcggcgcgg aatacatcta ttccgatgca tggactgaac gccataacca
     6001 gtctgaatcc tatgtgatta atgctgcagg aaagaaaact aaccatacca tttaccataa
     6061 aggtaaaggc agcctgggaa ttgacaacta cacgctgtat atggcggatc acattagctg
     6121 gcggaatgtg tcgttaatgc ccggtgtgcg ttatgactat gacaactatc tgtcaaacca
     6181 caatatctcc ccgcgcttta tgacggaatg ggatattttt gctgatcaaa cctcaatgat
     6241 taccgccggt tataaccgtt actatggcgg gaatattctt gatatgggat tacgtgatat
     6301 ccgcaatagc tggacggaat cggtatcagg taataaaacc ctgacgcgtt atcagaattt
     6361 gaaaacgcct tataacgatg aactggcaat gggattgcag caaaaaatcg ataagaacgt
     6421 tattgcacgc gcaagtgaag cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc
     6481 gactaaaacc accattactg aatataacaa cgatggcaaa accaaaacgc attcgtttaa
     6541 cctcagtttt gaactggccg aacccctgca tatccgccag gtagatatta acccgcaaat
     6601 tgtctttagc tatatcaaga gcaagggcaa cttgtcgtta aacaatggtt atgaggagag
     6661 caataccggt gataaccagg tggtttataa cggtaatctg gtctcttacg atagcgttcc
     6721 agtggcagat tttaataacc cattaaagat ctccttaaac atggatttca cgcatcaacc
     6781 gagcgggtta gtgtgggcga atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat
     6841 ccttggtaag acaaatgcgc aatacatcag cgaatattca gattacaagc agtatgttga
     6901 cgaaaaactg gatagcagcc tgacctggga cacccgcttg tcctggacgc cacaatttct
     6961 gaaacaacaa aacctgacga tcagtgccga tattctcaat gtactggata gcaaaaccgc
     7021 tgttgataca acgaacaccg gtgtggcgac ctacgccagt ggccgtactt tctggcttga
     7081 tgtcagcatg aaattttaat agttcggatt ttacttaacc gggtaaattc gcccggtttt
     7141 cgcatggaga taattatgag aaacctctgt ttcttactga cgttagtggc aactctgttg
     7201 ct
//
LOCUS       DAJVQR010000157         7178 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVQR010000157 DAJVQR010000000
VERSION     DAJVQR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            18..869
                     /gene="hchA"
                     /locus_tag="OQM52_003799"
     CDS             18..869
                     /gene="hchA"
                     /locus_tag="OQM52_003799"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS1565468.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(977..2335)
                     /gene="hprS"
                     /locus_tag="OQM52_003800"
     CDS             complement(977..2335)
                     /gene="hprS"
                     /locus_tag="OQM52_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1565469.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2335..3006)
                     /gene="hprR"
                     /locus_tag="OQM52_003801"
     CDS             complement(2335..3006)
                     /gene="hprR"
                     /locus_tag="OQM52_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1565470.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3139..3552
                     /gene="hiuH"
                     /locus_tag="OQM52_003802"
     CDS             3139..3552
                     /gene="hiuH"
                     /locus_tag="OQM52_003802"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1565471.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3661..4665
                     /gene="msrP"
                     /locus_tag="OQM52_003803"
                     /pseudo
     CDS             3661..4665
                     /gene="msrP"
                     /locus_tag="OQM52_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4666..5301
                     /gene="msrQ"
                     /locus_tag="OQM52_003804"
     CDS             4666..5301
                     /gene="msrQ"
                     /locus_tag="OQM52_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1565472.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5559..6209
                     /gene="zinT"
                     /locus_tag="OQM52_003805"
     CDS             5559..6209
                     /gene="zinT"
                     /locus_tag="OQM52_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1565473.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2027 a         1627 c         1506 g         2018 t
ORIGIN      
        1 acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc gatattgctg
       61 aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt cccgtctctg
      121 atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt ctggtgatcg
      181 ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc ggtaaccatc
      241 cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa ttcgaagtgg
      301 cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac aaagatgaaa
      361 aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag aaactcgcgg
      421 atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt cctggtgggc
      481 atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta cagtgggcaa
      541 tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt ctggcacttc
      601 gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac gccgcagaca
      661 aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc ggcgaagagt
      721 tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta cataaggacc
      781 gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa ctggcggcgc
      841 aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta agcgcataat
      901 caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt aacttatggc
      961 gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg ttatgcttac
     1021 tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg actaaagaaa
     1081 ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat ctacggaaga
     1141 gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta agagagccgt
     1201 tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat cgaatggcgt
     1261 taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag atttgttgat
     1321 tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga tagtccaaaa
     1381 gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca ttgtttttgt
     1441 ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc agctcttcaa
     1501 tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg agcgtaacct
     1561 gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga tcgtcagcaa
     1621 attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc aacgcctgcc
     1681 caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg ctatcgttat
     1741 aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc gtcctgatta
     1801 acaacggact taatactgag cacagtataa tggcgacaat gcaaattata atgctattaa
     1861 ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca gtaaccgtta
     1921 atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta tcattaataa
     1981 tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta tttaacatgt
     2041 catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga atgatcaaaa
     2101 tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct ggatttaccc
     2161 catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta tcatcgcgcc
     2221 atttcaactc acttgccagg ccattataga gagtccatac aattccagcg ccagcaacag
     2281 actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt ttcattattt
     2341 ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct tttcaggaaa
     2401 aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat tggtatcact
     2461 atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa tctcgccagc
     2521 tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa tactgatatt
     2581 gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt ctaatgttga
     2641 attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact cagaaaatga
     2701 aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc tgtcatcgac
     2761 agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta acgtttgtaa
     2821 gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg cataatcatc
     2881 cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga cataacccgc
     2941 ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa taagtagaat
     3001 cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt tcattacaaa
     3061 attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa aactcattca
     3121 catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc aacggcagcc
     3181 ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt gcacattttg
     3241 aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa gaaagcggat
     3301 aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat taaggcactg
     3361 tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac cggggactat
     3421 ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt tcatattaat
     3481 aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta ttcaacctat
     3541 cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag ggaggtgact
     3601 ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt gtgatggctt
     3661 atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt attctttatg
     3721 aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc tttgcctcac
     3781 gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc cgcccccgcc
     3841 ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc actgacgcca
     3901 gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa agacgatccc
     3961 gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag cggcgaagtg
     4021 gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct ggaagagcgt
     4081 atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat tggttttccg
     4141 ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt cgctttcgaa
     4201 acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg cggcgggctg
     4261 aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct cacactgatg
     4321 accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt gcgactgatt
     4381 gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa gctgacccgc
     4441 gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt ttacgccaac
     4501 gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt tattggttca
     4561 ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta cgccgaccag
     4621 gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg tctgacagca
     4681 aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc gtttctctgg
     4741 ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga tattcagcat
     4801 tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac acctctggca
     4861 cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt atggtgcttt
     4921 gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt gaataatctg
     4981 gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat tatcagctgg
     5041 gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa actcggcaaa
     5101 cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc aatacattat
     5161 ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct ggctgtactg
     5221 cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg caagcaggcc
     5281 cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat cccggccgga
     5341 tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt tcgtttgtca
     5401 tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca atacacctga
     5461 gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt acaatataac
     5521 attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt cttcataaac
     5581 tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat ggtcatcatt
     5641 ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt tttgatgata
     5701 ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc gtttaccctt
     5761 tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca gataaaacca
     5821 aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat atcgaaatga
     5881 ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca tcctgtaaat
     5941 atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc gttcgttact
     6001 tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt agcgatcata
     6061 taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat tcccagcaat
     6121 ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg agtagcgaag
     6181 aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt tatttaacag
     6241 aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga caaactcaga
     6301 cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac tgagaaaaag
     6361 gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt tccaggagca
     6421 agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt ttaaaccttt
     6481 tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa caactcttgg
     6541 aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt ttggctcaat
     6601 tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc agtttcatag
     6661 gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa ctgtagattt
     6721 tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc acagtatcga
     6781 acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct gtttaggaaa
     6841 agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag ggggatttga
     6901 acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga gtcgaaaaat
     6961 caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga aaaatcaatg
     7021 aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac ctgttttcag
     7081 gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct cagcttattc
     7141 cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAJVQR010000158         6913 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVQR010000158 DAJVQR010000000
VERSION     DAJVQR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..685
                     /locus_tag="OQM52_003806"
     CDS             <1..685
                     /locus_tag="OQM52_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1565474.1"
                     /translation="AICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKTTNQ
                     IHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELES
                     ELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYST
                     GGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVVACA
                     LANKLARIA"
     gene            complement(1024..1794)
                     /locus_tag="OQM52_003807"
     CDS             complement(1024..1794)
                     /locus_tag="OQM52_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565475.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1839..2273)
                     /locus_tag="OQM52_003808"
                     /pseudo
     CDS             complement(1839..2273)
                     /locus_tag="OQM52_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2285..2506)
                     /locus_tag="OQM52_003809"
     CDS             complement(2285..2506)
                     /locus_tag="OQM52_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565476.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2507..3190)
                     /locus_tag="OQM52_003810"
     CDS             complement(2507..3190)
                     /locus_tag="OQM52_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS1565477.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3254..3565)
                     /locus_tag="OQM52_003811"
     CDS             complement(3254..3565)
                     /locus_tag="OQM52_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565478.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3562..4464)
                     /locus_tag="OQM52_003812"
     CDS             complement(3562..4464)
                     /locus_tag="OQM52_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCS1565479.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4904..5863
                     /locus_tag="OQM52_003813"
     CDS             4904..5863
                     /locus_tag="OQM52_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCS1565480.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5863..6258
                     /locus_tag="OQM52_003814"
     CDS             5863..6258
                     /locus_tag="OQM52_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCS1565481.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6441..6821
                     /locus_tag="OQM52_003815"
     CDS             6441..6821
                     /locus_tag="OQM52_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCS1565482.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1889 a         1790 c         1673 g         1561 t
ORIGIN      
        1 agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa gaactgagac
       61 ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag ataaggtgaa
      121 aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc cgacagggga
      181 tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg aatatctgag
      241 ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa tagcagagct
      301 ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca ttatgacaat
      361 tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg acgggaaaca
      421 gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc agtatagcac
      481 tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt tacgccggtt
      541 actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg gaaaacttgc
      601 tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct gcgctctggc
      661 aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat atcaggcctg
      721 atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata caagttaccg
      781 tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct gattaaaaac
      841 ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc aggtgcggaa
      901 ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta agcaagccca
      961 ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc cccgccatcg
     1021 tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg cccgggcttc
     1081 ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt catccggtat
     1141 acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat cgctgaccag
     1201 atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga gcaccacggg
     1261 caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca cgaccaggcg
     1321 aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg ccgcccggat
     1381 acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc gcaccgcatc
     1441 aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga aagacacctg
     1501 cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca gaacaagttc
     1561 cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca gcacgccatc
     1621 gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt aaatgctgat
     1681 ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc agccctgcgc
     1741 cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat tcataattca
     1801 tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc gtgtgtgttg
     1861 tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac gtattccggg
     1921 agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg ttcagcgatc
     1981 tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc gcgcacgttg
     2041 ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta gtcttccggg
     2101 atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga aatatccatt
     2161 ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag cggcgtgtct
     2221 cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta catactctct
     2281 cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc tccatcaaga
     2341 ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg tcagcattaa
     2401 agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg gcattggcca
     2461 cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac gcagcctccg
     2521 gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg gcagccagtc
     2581 gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac cggcgtccgg
     2641 actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc actatcgcat
     2701 tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt tccgtcgggt
     2761 gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt tgtggcagac
     2821 gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca tatgcggcct
     2881 tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc gcatttttcc
     2941 aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc agcgcgtctt
     3001 ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca gttttatcac
     3061 cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga tcggtgagga
     3121 tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta ccgaggacaa
     3181 aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc gttccccgct
     3241 gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa taccgcagcc
     3301 gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt tcatcagaaa
     3361 agggcgacgg gctggcgtac actgtcaccg acaccagccg ggagcggtaa aagcgcccgt
     3421 cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc gccgcgtcag
     3481 ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa cgggcggttg
     3541 cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga ctatccacgc
     3601 aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt caagttcccc
     3661 ccacagcaca ttgacgagct ctccacgctc atacaactgc cagccacaga aatcacagcc
     3721 ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt ctgcaataac
     3781 gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt tttctccctg
     3841 actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt ccgtatacca
     3901 gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca cttcaacatc
     3961 caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg gcactacgtt
     4021 gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt cacaccagtc
     4081 acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg gtaatccttc
     4141 ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca ggcagttttg
     4201 ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg cgatattttc
     4261 cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag agcggatatt
     4321 ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac cttcatttgt
     4381 ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct cagcgatctg
     4441 tgccggctca ccaggaaaat acatacgatt cgagcaccag tttggcatgg gaattctcct
     4501 ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg catacgaatg
     4561 caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg accgggtgag
     4621 gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg ccggcacggc
     4681 ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat aagaactcaa
     4741 aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt gacatagact
     4801 caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga cataaaggat
     4861 gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa aagtatcatg
     4921 cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg taagaaccag
     4981 tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg ccggcaaagt
     5041 ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca ctgctgtcat
     5101 aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca tacatcatgc
     5161 actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat tacctgtgac
     5221 cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa aaaaggcaaa
     5281 tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa aagttaatgt
     5341 aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag taaacaaact
     5401 tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt taattctcgg
     5461 tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat cccgcatcac
     5521 tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt ttattgctga
     5581 tgagttaata aaaaacagac acgataatga atatctccag acattaataa atgatgtagc
     5641 tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg cagaaagcat
     5701 caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg gcggaggagc
     5761 cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga acaaggtgga
     5821 tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag cagtgatcca
     5881 aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca ggacaacctg
     5941 acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta cagacaatcc
     6001 cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct aatcagcgtt
     6061 tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca aacaacagga
     6121 tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc atcttcagaa
     6181 aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag acgcaatctt
     6241 tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc tcttctttta
     6301 tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa tgcaacagac
     6361 aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca ttattgtcag
     6421 tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt gatgatgata
     6481 taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact caggctattg
     6541 cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc acctcaattg
     6601 taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa gctcttgcag
     6661 tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa gcaaagctaa
     6721 tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa ataggtcttc
     6781 tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag ttatagtaac
     6841 tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat gaggcatgga
     6901 ccatcatcaa gcc
//
LOCUS       DAJVQR010000159         6785 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVQR010000159 DAJVQR010000000
VERSION     DAJVQR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            230..2662
                     /locus_tag="OQM52_003816"
     CDS             230..2662
                     /locus_tag="OQM52_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1565483.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2729..4198
                     /locus_tag="OQM52_003817"
     CDS             2729..4198
                     /locus_tag="OQM52_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1565484.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4198..5859
                     /locus_tag="OQM52_003818"
     CDS             4198..5859
                     /locus_tag="OQM52_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1565485.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6080..6421
                     /gene="symE"
                     /locus_tag="OQM52_003819"
     CDS             6080..6421
                     /gene="symE"
                     /locus_tag="OQM52_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCS1565486.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6468..>6785)
                     /locus_tag="OQM52_003820"
     CDS             complement(6468..>6785)
                     /locus_tag="OQM52_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565487.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1610 c         1553 g         1619 t
ORIGIN      
        1 gaaagaaacg caactataag atttttatat attttgttag agttgatatg gttcctgtaa
       61 cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata acagagatat
      121 caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat agccaggcta
      181 ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca tggcggaact
      241 gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc cagccattct
      301 caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc gggacggtaa
      361 agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg acatcgtgct
      421 gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca aacatgaaat
      481 tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc cttttgtttt
      541 tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag gtgaatgcct
      601 tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc aaaaattctg
      661 cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact attacgacga
      721 tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa ccattgaagc
      781 cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag ggaaaaccta
      841 taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt
      901 ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc agccatttgg
      961 tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa ttcacctcgc
     1021 gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc
     1081 agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat ccgaagacag
     1141 cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct taaccgccac
     1201 gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg tttacgtcta
     1261 ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg tccgtgttga
     1321 tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact taaacggcga
     1381 agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg tgatcgacga
     1441 acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca atccgatgga
     1501 taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt
     1561 taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga
     1621 tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac cgtacccggt
     1681 tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct gcaaactggt
     1741 agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg gtcgtggtac
     1801 acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta aaaaagccac
     1861 cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg ataccacacc
     1921 agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca
     1981 tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc aggttaccga
     2041 taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca agtttcacgt
     2101 caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg ccgacggtaa
     2161 actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag aatatgcctc
     2221 gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga tcattcacga
     2281 gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta aagatctcga
     2341 cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg
     2401 cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag cgcaagccgt
     2461 gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg aaagtattca
     2521 ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta ttaaaaccgg
     2581 atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg aaatctacca
     2641 attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca tcatgttgca
     2701 gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca aatcattaca
     2761 agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg gtcagctctc
     2821 ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac tggagcagga
     2881 taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg ccaacgcgca
     2941 gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc cggcgttgaa
     3001 aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc aggcgttcag
     3061 cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca acaagctgaa
     3121 cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt acgaacagat
     3181 ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac gcgccgtcac
     3241 tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg acccggcctg
     3301 cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat acgtgaagag
     3361 cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa aacagcttcc
     3421 gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg tacaaattcg
     3481 tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg atgtcattgt
     3541 taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact ttccggcaga
     3601 gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag tactggcgaa
     3661 aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag gtgttaaaac
     3721 caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc gtttaccgaa
     3781 tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta ccaaaggtca
     3841 gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga aaaactacag
     3901 caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt ggggtaacga
     3961 ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca gcattgatga
     4021 cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg aaaccgtcag
     4081 ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga tccagacgct
     4141 gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg ttaactaatg
     4201 agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt
     4261 tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa gaaattgcgt
     4321 gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc gaatgatgaa
     4381 ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt gaagcaaggc
     4441 aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc gtttgaattg
     4501 ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc tgagtcatct
     4561 agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat cgagcaaagc
     4621 caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt aaataatcca
     4681 gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga ttttgttgtc
     4741 ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt ctttttttgg
     4801 cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa agtattaaat
     4861 agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga aaaagttagc
     4921 tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct ggacgcacat
     4981 cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac cgcggaactg
     5041 gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac cgaagccagc
     5101 gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag
     5161 gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga aaaagctcaa
     5221 ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag cgatgaggaa
     5281 aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat agttaatatt
     5341 aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc acctgatatt
     5401 attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc aggagtcaaa
     5461 gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg cccgtcatta
     5521 tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta tccattagac
     5581 tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt tcttatgcaa
     5641 gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag tttttacaat
     5701 attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa gataaattct
     5761 attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa aacccaactg
     5821 catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc tcacaaaacc
     5881 aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt cacgctaatt
     5941 ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga tgatgtatcc
     6001 aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc acaatcacta
     6061 tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt tagaagcaga
     6121 agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt acccggatta
     6181 cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg gttttgccac
     6241 cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg cccaacccgc
     6301 cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc tgtcggcgcg
     6361 taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga aagtcgcctg
     6421 atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta taccaacgcg
     6481 ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg aagttgtgcg
     6541 aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt tgtcacatct
     6601 ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga caatcgcagt
     6661 cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc aatatatagc
     6721 ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg aagatgatcg
     6781 aataa
//
LOCUS       DAJVQR010000160         6677 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVQR010000160 DAJVQR010000000
VERSION     DAJVQR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(286..1188)
                     /locus_tag="OQM52_003821"
     CDS             complement(286..1188)
                     /locus_tag="OQM52_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCS1565488.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1267..2220)
                     /gene="lsrR"
                     /locus_tag="OQM52_003822"
     CDS             complement(1267..2220)
                     /gene="lsrR"
                     /locus_tag="OQM52_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCS1565489.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2469..4004
                     /gene="lsrA"
                     /locus_tag="OQM52_003823"
     CDS             2469..4004
                     /gene="lsrA"
                     /locus_tag="OQM52_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCS1565490.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDHATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3998..5026
                     /gene="lsrC"
                     /locus_tag="OQM52_003824"
     CDS             3998..5026
                     /gene="lsrC"
                     /locus_tag="OQM52_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCS1565491.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5026..6018
                     /gene="lsrD"
                     /locus_tag="OQM52_003825"
     CDS             5026..6018
                     /gene="lsrD"
                     /locus_tag="OQM52_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCS1565492.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6030..6677
                     /gene="lsrB"
                     /locus_tag="OQM52_003826"
                     /pseudo
     CDS             6030..6677
                     /gene="lsrB"
                     /locus_tag="OQM52_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1526 a         1707 c         1743 g         1701 t
ORIGIN      
        1 agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc aggctatttc
       61 tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc tgaacgaata
      121 acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat atacattatg
      181 cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata gacatgtccg
      241 cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg acggcatacg
      301 gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct tattatccgt
      361 gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc caaggcaacc
      421 aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac cgcagagttc
      481 cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag gagaaagaat
      541 atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg tggttagatc
      601 aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc tgagcatata
      661 ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat cggaacgatg
      721 gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc cggtcgctcg
      781 ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa cttcgcgtgc
      841 cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt tatataaaac
      901 aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt ctatgccggc
      961 gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt tgagatcaaa
     1021 ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct gtcccactgc
     1081 tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc ctgcatccag
     1141 cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta gattatcctc
     1201 ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta ataaggtcat
     1261 gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca gtgcgttgat
     1321 ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc ctgccacgcc
     1381 aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt cgttatgtat
     1441 tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa tgtcgccaac
     1501 cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg agcgaatgat
     1561 tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat ccgctgcttg
     1621 cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg caatgtcagc
     1681 ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt taagctgccc
     1741 gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa tttgctgtga
     1801 cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa aaccaatcgc
     1861 cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta tccctagtcg
     1921 cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca gcgaaaactg
     1981 acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa tctgtacgcg
     2041 aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac gggtcaggcc
     2101 gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc acgcgatccg
     2161 cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt tgattgtcat
     2221 aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag ttcttgcgca
     2281 tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac gcaaccgtga
     2341 aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg tccacctaag
     2401 gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca aaactgaacg
     2461 ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc cgctcggttt
     2521 ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg catcaggggg
     2581 aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg aagattattg
     2641 ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac tacgccagat
     2701 taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa ccgctgcttt
     2761 tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa cagctctcca
     2821 tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg catagtctgg
     2881 caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg ctgatgcgcg
     2941 actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg gaaaccgaac
     3001 gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt tttatctcgc
     3061 ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc gacggaacca
     3121 tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag gccatcaccc
     3181 cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag ttacctggta
     3241 accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg actggcgaag
     3301 gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg gctgggctgg
     3361 tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact ttgcgtggcg
     3421 gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa cgtttactgc
     3481 gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc gatgcttcgc
     3541 tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg aaaaccgcga
     3601 aagatcatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt aaccaaccgg
     3661 aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt gccaaatgcc
     3721 tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg gatgtctcgg
     3781 cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg gctgtgctgt
     3841 ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat gtgatgcatc
     3901 agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag actattatgc
     3961 gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta ttcagaacaa
     4021 ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg gttttctcga
     4081 ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc aaatcctgat
     4141 cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg tttcagtggg
     4201 ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag gatattcact
     4261 acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt tcaacggtgc
     4321 cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt tagggttgta
     4381 cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac ccgccgaact
     4441 gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt tgacgataat
     4501 tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca gtttttatgc
     4561 cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag ccattcgcat
     4621 tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt ttgcttcgca
     4681 gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa ttgcagcctg
     4741 cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg cggtactcgg
     4801 cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc cggcatggtg
     4861 gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg gacgcctgcg
     4921 ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga cgccaccgcc
     4981 atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg tattcgctac
     5041 ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt tggtgcaatt
     5101 aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt tatctgcatt
     5161 ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga tatttcgttt
     5221 ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag tggtgtgccg
     5281 atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct gatcaacgcc
     5341 ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac gctgtatctg
     5401 tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta cgaaggtatt
     5461 ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg actccccgtt
     5521 ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca taaaacccat
     5581 gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta tagcgcgatt
     5641 ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc tgcggtcgcc
     5701 gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc gtcgtttctg
     5761 atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg ttccggttcc
     5821 attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg tttgcaaatg
     5881 gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat cgtcgttgtc
     5941 gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc gcgtcgggcc
     6001 aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag aaaatcgcct
     6061 tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca gagcgtattg
     6121 catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac ggcgcacaac
     6181 aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa cccagtgttt
     6241 ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc attattcgtt
     6301 tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca acgtggtgtg
     6361 agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta cattaatcag
     6421 ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca ggtgaataaa
     6481 gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca aaaccagtgg
     6541 gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat tgtcactacg
     6601 cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat attaaaagcg
     6661 tatagcgatc tcgacgc
//
LOCUS       DAJVQR010000161         6633 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVQR010000161 DAJVQR010000000
VERSION     DAJVQR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..799
                     /locus_tag="OQM52_003827"
     CDS             <1..799
                     /locus_tag="OQM52_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1565493.1"
                     /translation="DRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTV
                     LSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNV
                     TLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSRKSVLFWIL
                     TALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFI
                     AIDMDKLKQINDTLGHQEGDLAITLHDAYKASDERLYVNKQNKNSRS"
     gene            842..1345
                     /gene="yeaK"
                     /locus_tag="OQM52_003828"
     CDS             842..1345
                     /gene="yeaK"
                     /locus_tag="OQM52_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCS1565494.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1346..1450)
                     /locus_tag="OQM52_003829"
     CDS             complement(1346..1450)
                     /locus_tag="OQM52_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565495.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1620..2066
                     /locus_tag="OQM52_003830"
     CDS             1620..2066
                     /locus_tag="OQM52_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCS1565496.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2023..2841)
                     /gene="nimR"
                     /locus_tag="OQM52_003831"
                     /pseudo
     CDS             complement(2023..2841)
                     /gene="nimR"
                     /locus_tag="OQM52_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            2938..4118
                     /gene="nimT"
                     /locus_tag="OQM52_003832"
                     /pseudo
     CDS             2938..4118
                     /gene="nimT"
                     /locus_tag="OQM52_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4173..4520
                     /locus_tag="OQM52_003833"
     CDS             4173..4520
                     /locus_tag="OQM52_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCS1565497.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4542..4796)
                     /gene="yoaF"
                     /locus_tag="OQM52_003834"
     CDS             complement(4542..4796)
                     /gene="yoaF"
                     /locus_tag="OQM52_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCS1565498.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4979..6004
                     /gene="dgcP"
                     /locus_tag="OQM52_003835"
     CDS             4979..6004
                     /gene="dgcP"
                     /locus_tag="OQM52_003835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCS1565499.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6037..6135
                     /locus_tag="OQM52_003836"
     CDS             6037..6135
                     /locus_tag="OQM52_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCS1565500.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6138..6212
                     /gene="yoaJ"
                     /locus_tag="OQM52_003837"
     CDS             6138..6212
                     /gene="yoaJ"
                     /locus_tag="OQM52_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCS1565501.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6373..6564)
                     /locus_tag="OQM52_003838"
                     /pseudo
     CDS             complement(6373..6564)
                     /locus_tag="OQM52_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1592 a         1610 c         1621 g         1810 t
ORIGIN      
        1 tgaccgctat gtctattttt atgagccggt taatgttgaa tactttgcga tgaataactg
       61 gtccttcctg cagtcaggaa gtattggcat cgatcgcaaa gatattgaaa aggtatttac
      121 cgggcgtacc gtattgtcga gcatttacca ggatcagcgt actaaacaga acgtgatgag
      181 tttgctgacg ccggtatatg tcgcagggca gctaaaaggg attgtgctgc tggatattaa
      241 caaaaacaat ttgcggaata tcttttacac tcatgaccgc cctctcctct ggcgttttct
      301 caatgtcacg ctaaccgata ccgattcggg gcgcgacatt atcatcaacc agagcgaaga
      361 taatctgttc cagtatgtca gttacgtcca tgacttacct ggcggcattc gtgtctcgtt
      421 atccattgat attctttact ttatcacgtc ttcgaggaaa agcgttctgt tctggatttt
      481 gacggcgtta attttgctga atatggtccg gatgcacttc cgtttatacc aaaatgtgtc
      541 gcgagaaaat attagtgatg cgatgactgg actgtataat cgcaaaattt taacccctga
      601 actggagcag cggttgcaga aactggtgca atccggttct tcggtgatgt ttattgcaat
      661 tgacatggac aagttaaagc aaataaatga caccctcggt catcaggagg gggatttagc
      721 gattacgtta catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa
      781 caaaaacagc cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcgtga
      841 aatgactgaa atggctaaag gaagcgtgac gcatcagcag ttaatcgctt tattatcaca
      901 agaaggtgct aacttccgcg tcgttaccca tgaagcggta ggtaaatgtg aagcggtgtc
      961 agaaattcgt ggtaccgctc taggccaggg tgctaaggca ctggtatgta aagtcaaagg
     1021 caatggcgta aatcaacatg ttctggcgat cctcgcagcc gatcaacagg ccgatctgag
     1081 ccaactcgcc agccatatag gcggattacg cgcttcgttg gccagcccgg cggaagttga
     1141 tgaactcaca ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa
     1201 actggttgcc gaccctctac tcttcgaacg atttgatgaa atcgccttca atgcagggat
     1261 gctggataaa tccgttattt tgaaaaccgc ggattatttg cgcattgcgc aaccagaact
     1321 ggtcaatttc cgccgcactg cgtagctagc ctatccgttc aataagcaga acggacaaaa
     1381 ccagcacaac cgcaatagca aaaaacgatg atgtgataat cagtgtctcg acgaacattt
     1441 gatcgttcat agcatgcccc cgggagatag ctgttttcac gttattgtta gcgtgcacaa
     1501 atggcagttt gatgacagtt cgcctgattt tttatgcaaa aaacgtaaag attttctact
     1561 tccttcctgc agcaagcata aagtaagcag gcttattatt ttttggcaag gaaaccacga
     1621 tgtttgatgt cactttgctg atcctgctcg ggttagctgc gctgggcttt atcagtcata
     1681 acaccactgt cgccgtttca attctggtgt tgatcattgt ccgcgtcaca ccgttaagca
     1741 cctttttccc gtggattgaa aaacaggggc taagtatcgg gataatcatt ctgacgattg
     1801 gcgttatggc ccccatcgcc agcgggacgc taccgccctc aaccttgatt cactcgtttc
     1861 tgaactggaa atcgctggtg gcaattgcag taggcgttat tgtctcctgg ctgggtgggc
     1921 gcggcgtgac gttaatgggc agccagccgc aactggttgc tgggctgctg gtgggcactg
     1981 ttttaggcgt agcgctgttt cgcggcgtac cggttggccc gcttattgcc gcgggtctgg
     2041 tttcgctgat tgtggggaaa cagtagttaa tcccgcaata tatcggcctg gcgtttgccc
     2101 caagcctttc ttaaacatag taataaaggc cgttgttgaa tcatacccca gcgtatgagc
     2161 aactttctgt acagtatcgc ctttcactaa tccctgaagc gccataatca gctgtaactg
     2221 ctgacgccac tggcgaaaac tcaaccccgt ctctttcact atcaggcggg caagattgcg
     2281 ttcactcatc gcaaaaaagc cagcccattg ccccaatgcc ccccactcga cgggcccttt
     2341 cgccatcatc tccaccatcg tgcggatttt aggatgagaa gaaacgggca ggtgcagctg
     2401 ttgttgtggc tgctgaggga gttcatcaaa caggacctga attaatctgc gggtcatcgg
     2461 ctcagcacgc tgagtcgtcg tcctgtttgc cagggttaaa atcaattccc ggcataaggg
     2521 cgatattttc aatgtgcaac atggtcggca ttgtcactgc gctgggttcg ataaacagaa
     2581 aacagagttc agcgttagct gtcacttgat tgctatgctt caccccgccc ggtatccaaa
     2641 ccgcatattg tggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat
     2701 gtaatgccag tatcagctgt cctttgcggt gctgatgtac aggactggtt aactcatccg
     2761 ttccggcgtg aatacaaaat gcgaccgcgg cttcatggtg tcggtgggtt catagccatt
     2821 gagattcaac ctgtgcatca ttttgtccga acttagcgat aatttgtcat tttagcttga
     2881 ttcaacataa caataaaaac ggtaaggtac agcctcgttt gtaacaatga gaagcatatg
     2941 acctgttcaa cttcattaag cggcaaaaac aggattgtcc ttatcgctgg cattctgatg
     3001 attgccacaa cattacgcgt cacctttacc ggcgcagcgc cgttactgga tacgattcgt
     3061 tccgcttact cgctgacgac agcgcaaacc ggcttattaa ccaccctgcc attattggcc
     3121 tttgcgctaa tctcacctct ggctgccccg gtggcgcgac gttttggtat ggaacgtagc
     3181 ctgtttgccg cgttactttt gatctgtgca ggtatcgcaa ttcgttctct cccttcgcct
     3241 tacttattat ttggcggtac agcggtcatt ggcggtggga ttgcattagg caatgtctta
     3301 ctgccaggat taattaaacg cgatttccct cattccgtcg ccagacttac cggcgcatat
     3361 tccctgacaa tgggagctgc agcggcactg ggatcggcta tggtcgtgcc gctggctttg
     3421 aacggttttg gctggcaagg cgcgttgttc atgctgatgt gttttcctct gctggctctt
     3481 tttttatggc tgccacagtg gcgaagtcag caacatgcaa atttgagtac ctcacgcgcc
     3541 ttacatactc ggggtatctg gcgttcgcca cttgcctggc aggtaacatt gtttcttggg
     3601 atcaactcac tggtctatta cgtgattatt ggctggcttc cggcgatcct catcagtcac
     3661 ggctatagcg aagcacaggc gggttcactg catggtttgc tgcaactagc cacagcagct
     3721 cccggtttgc tgatcgcact tttcttacat catgtgaaag accagcgtgg tattgcagcg
     3781 ttcgttgcct tgatgtgcgc agtgggcgcg gttgggctct gctttatgcc agcgcacgcg
     3841 atcacctgga ctctgctttt cggttttggt tccggcgcaa caatgatact ggggttgacg
     3901 ttcattggtc ttcggcaagt tctgcgcatc aggcggcggc actctcgggg atggcacaat
     3961 ccgtcgggta tttgctggca gcctgtgggc cgccgctaat gggtaaaata cacgatgcta
     4021 acggtaactg gtccgtacca ctgatgggtg ttgcaatact ttcactactg atggcgattt
     4081 tcggactttg cgccgggaga gacaaagaaa ttcgctaata tccggtgcta tagtgacgtg
     4141 acaaatcttg cgtgaaaggg agaacaaaca cgatgaatat tcagtgcaaa cgcgtttatg
     4201 atccggctga acagagcgat ggttatcgcg tactggtcga ccgcctctgg ccgcgcggta
     4261 tcaaaaaaac cgatttagcc cttgatgagt gggataaaga aatcacgccg tcaacggaac
     4321 tgcgcaaagc ctttcacggc gaagtcgtcg attttgcaac ctttcgcgag caatatcgcg
     4381 cagaactggc gcaacacgag caagaaggaa agcggctggc ggacatcgcc aaaaaacagc
     4441 cgctgaccct gctctacgca gcaaaaaaca ccacgcagaa ccatgcgctg gtgctggccg
     4501 actggctacg tagcttgtga ttttagtacg gcatccggcg gttatttttc accagccgga
     4561 tggtcccgtc gccacaatgc ccattcatca atcgtttcac cgcccggtaa tttgcaattg
     4621 ttgctgaccc cttgcgctgt ctgcactgga atgagcgtcc cgcccttctg ctggcaatag
     4681 accgacgccg gatttgccat accaatctgc ggcggtttag gtgcttctgg ctgagaaggg
     4741 gttgaacaac cagccaggac cagcaagcaa ggcagaacaa aactgataat tttcatttat
     4801 tgatctcaca tatttatcca agcttagagt atcgcggtat cgttttgttt tgcagcacta
     4861 tttttattac attcactcaa aacatattac gtcttgtttc atctttgttg atgatgtttt
     4921 atcatgcctg caaagattaa ataatcagca cttacccgcc gtatcctgga gttgttccgt
     4981 gtcagatcag attatcgccc gcgtctcgca atcccttgcc aaagaacagt cactggaaag
     5041 tctggtccga cagcttctgg agatgctgga aatggtcact gatatggaat caacctacct
     5101 gaccaaagtg gatgtcgaag cgcgcctgca gcatattatg tttgcccgta acagccagaa
     5161 aatgcacatc ccggagaatt ttaccgtctt gtgggattac tcgttatgca aacgcgccat
     5221 tgatgaaaac tgctttttca gcgatgaagt ccctgaccgt tggggcgact gtattgcggc
     5281 acgcaatctt ggcatcacca catttctgag cacgccaatt cacttaccgg atggatcatt
     5341 ctatggcacg ctttgcgccg ccagcagtga gaagcgccag tggagtgaac gcgcggaaca
     5401 ggttttacag ttattcgccg gactgattgc acaatatatt caaaaagagg cactggttga
     5461 acagctgcac gaagccaatg ctgcgctgat tgcgcaatcg tataccgact cgttaaccgg
     5521 gctaccgaat cggcgggcga tttttgaaaa tctgacgacg ctgttttccc tcgcccggca
     5581 tcttaaccat aagataatga tcgcgtttat cgatctggat aacttcaaat taatcaatga
     5641 tcgttttggt cataatagtg gcgatctgtt tctcattcag gttggcgagc gccttaatac
     5701 gctccagcaa aatggcgaag ttattggtcg tctcggtggt gatgagtttt tggttgtttc
     5761 actaaacaac gagaatgcgg atatttcgtc gctgcgagaa cgtattcaac agcaaatacg
     5821 tggagaatat cacttaggtg atgttgattt gtattatccc ggtgccagtc ttggcatagt
     5881 agaagtcgat cctgaaacaa ccgatgcaga cagtgccctg catgctgccg atattgcgat
     5941 gtatcaggag aaaaaacaca aacagaaaac accttttgtc gcgcatccag cgctacattc
     6001 ctgaggcgta ttcacatcct tttgattggt gataacatgc gaatcggtat tatttttccg
     6061 gttgtaatct tcattacagc ggtcgtattt ttagcatggt tttttattgg ctgctatgct
     6121 gccccgggag cataaagatg aaaaaaacaa cgattattat gatgggtgtg gcgattattg
     6181 tcgtactcgg cactgagctg ggatggtggt aacgtcacct ctaaaaaata gcaaaggctg
     6241 cctgtgtgca gcctttgtgc aatttaagcg ttaactttta atcttcctgt agataaatag
     6301 cacgacaatc gcaccaataa cggctgaagc ggcgcacgaa aaacgctttc gcgtttttcg
     6361 tgcgccgctt cagccctggt tcagcatttc gtctgcagtc tgaaccgcag gcaaccattt
     6421 ttcagcaagt ctgcgctggg aatcgctggc tttatcccat ttgctccaca gtgcctcacg
     6481 atcgtaatcg tgcgcttcca gcgtggggcg ggcgatttca aaatacccct agcttgtttc
     6541 tatttgctca gattttaata acaactcagc acgtgctgcc gctggtagaa ctgatatatg
     6601 aaattccaaa gcctttgaac ccgtacgctt gcg
//
LOCUS       DAJVQR010000162         6511 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVQR010000162 DAJVQR010000000
VERSION     DAJVQR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            429..1817
                     /gene="narU"
                     /locus_tag="OQM52_003839"
     CDS             429..1817
                     /gene="narU"
                     /locus_tag="OQM52_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS1565502.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1899..5639
                     /gene="narZ"
                     /locus_tag="OQM52_003840"
                     /pseudo
     CDS             1899..5639
                     /gene="narZ"
                     /locus_tag="OQM52_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5636..>6511
                     /gene="narH"
                     /locus_tag="OQM52_003841"
     CDS             5636..>6511
                     /gene="narH"
                     /locus_tag="OQM52_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1565503.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1554 a         1713 c         1688 g         1556 t
ORIGIN      
        1 ataaacaata cttactataa taacgattat tacaatcagg caataagaga ggaaccgata
       61 gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat aaatcatcac
      121 taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc tttagattca
      181 cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt gaaggttagc
      241 ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg cccccgcggt
      301 tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt aattctaata
      361 actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt gtgcatatga
      421 ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc gactggaaac
      481 cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga aacctctgga
      541 tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc gcagttaccg
      601 ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta ttaaccgcat
      661 taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg cctatattcg
      721 gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc gtctggctcg
      781 gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc gctttgctat
      841 gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc ttctttccaa
      901 aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta ggtgtaagtg
      961 taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt ctcggcgtca
     1021 atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca tggatttggg
     1081 taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat atcgccagtt
     1141 cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc tggctgctga
     1201 gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc
     1261 tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt ggcccattta
     1321 ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc ggcgtgcggg
     1381 tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc cttaccttac
     1441 cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg ctgtttctga
     1501 ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt cgccagataa
     1561 ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga gaagccgtca
     1621 ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc ggctttttta
     1681 ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc gcgatgaaag
     1741 tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat ggtcggcgga
     1801 agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat ccgacaaggc
     1861 atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt ttggatcgct
     1921 ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag gtgatgcata
     1981 gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt
     2041 ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt aaaaatggtc
     2101 tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac ctgcccaatc
     2161 atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac agcgctaacc
     2221 gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa gccctcaaac
     2281 aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa aagtgcctga
     2341 gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag gaactaaacc
     2401 agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat cgcgttgccg
     2461 gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg cgttatctgt
     2521 cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg ccgcccgcct
     2581 cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg tataactcca
     2641 gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac gcccacttct
     2701 ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac tactctgaag
     2761 tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc gccctggcga
     2821 tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc gactacttta
     2881 tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag cctcgcgacg
     2941 atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat ggactgggcg
     3001 aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa ttggtagtgc
     3061 cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg gaatccattg
     3121 ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac gctgttgcag
     3181 gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc gtaaaacaca
     3241 atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat ggcaacacct
     3301 gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat cgcgggctgg
     3361 aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca gcctggggtg
     3421 agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa tttgccgata
     3481 ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt aaccactggt
     3541 atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc ggttgtgtcg
     3601 ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc ccacaaaccg
     3661 gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag atgaacagca
     3721 cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct gctcaggagt
     3781 tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat ttcaacgttc
     3841 gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac ccgctcggga
     3901 ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc caggcgctga
     3961 aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat catccgcgta
     4021 atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac gagtatatac
     4081 agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt gccagcgacg
     4141 ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc
     4201 tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc gttctgccca
     4261 ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca tttattcatc
     4321 cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa atctacaaag
     4381 gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa accgacgtgg
     4441 tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt gaagtgctcg
     4501 actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat attgtggcgg
     4561 tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca ttgatggaca
     4621 aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc gatttcctcg
     4681 gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg attgacaccg
     4741 ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt catgttgcag
     4801 tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat ctggcgctgc
     4861 acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt aaaattatct
     4921 ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg ggatacacca
     4981 acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc
     5041 atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc
     5101 gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa gcacttaact
     5161 tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac ctgctaatgc
     5221 tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc cgagaactga
     5281 ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg actgcccgcg
     5341 cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc caggaacgca
     5401 ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat aactcggtca
     5461 cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg gcctggggct
     5521 ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc cgcaagatga
     5581 agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa
     5641 aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct gccatacctg
     5701 ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg catggtttaa
     5761 caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg
     5821 gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg gcagcaagat
     5881 gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg atgattacta
     5941 cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca aacatatccc
     6001 tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc
     6061 aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga
     6121 ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg
     6181 cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg gcgctatcta
     6241 caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt
     6301 gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga
     6361 aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac
     6421 ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc gcatcgagga
     6481 agcgtcgagc accgagcgcg aagttgacct c
//
LOCUS       DAJVQR010000163         6328 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVQR010000163 DAJVQR010000000
VERSION     DAJVQR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            79..258
                     /locus_tag="OQM52_003842"
                     /pseudo
     CDS             79..258
                     /locus_tag="OQM52_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            783..1961
                     /gene="ltrA"
                     /locus_tag="OQM52_003843"
     CDS             783..1961
                     /gene="ltrA"
                     /locus_tag="OQM52_003843"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1565504.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2044..2223
                     /locus_tag="OQM52_003844"
                     /pseudo
     CDS             2044..2223
                     /locus_tag="OQM52_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2253..2735)
                     /locus_tag="OQM52_003845"
                     /pseudo
     CDS             complement(2253..2735)
                     /locus_tag="OQM52_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2991..3971)
                     /locus_tag="OQM52_003846"
     CDS             complement(2991..3971)
                     /locus_tag="OQM52_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCS1565505.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3971..5170)
                     /locus_tag="OQM52_003847"
     CDS             complement(3971..5170)
                     /locus_tag="OQM52_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1565506.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5492..5830)
                     /locus_tag="OQM52_003848"
                     /pseudo
     CDS             complement(5492..5830)
                     /locus_tag="OQM52_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5897..>6328)
                     /gene="istB"
                     /locus_tag="OQM52_003849"
     CDS             complement(5897..>6328)
                     /gene="istB"
                     /locus_tag="OQM52_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCS1565507.1"
                     /translation="HLAIAMGYEAVRVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1712 a         1463 c         1509 g         1644 t
ORIGIN      
        1 gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact
       61 ctccaggaaa cccggggcgg ttcagttgct gaactgctgg aaccgctgta tgacatcctg
      121 cgccagtatg tgctgatgcc cggtaaagtc catgccgatg atatccccgt tccggtccag
      181 gagccgggca gcggtaaaac aaggactgcc cggctgtggg tctacgttcg tgatgaccgt
      241 aacgccggtt cggaaattgc agcataagtt atccatgctc tgaggtgggt agaggtgggc
      301 cctccggtca gggtttacag gagcacagac caaacgaccc ggtgctgctt cattctaagg
      361 aatgaggtgg tgagcgactc gggaaaaggc agagatctac tgtcacgtgg gttcgggaag
      421 ctgaacgaaa gtaaactgtc cgatgaagca tcgaaatctt agatgatgcc aaaatggggc
      481 cgtacagccg tcccctgata agcgcagaag acacctgctt actgactgcg cggcatccgg
      541 tgtgaaggca gcaggatccc gggccagacc atctcaggga acgtgagaac ctgtttccgg
      601 cacgtatcgg gaaacagggg cggacccatt cgtagtagtg atgaaagatc tgtaatgggt
      661 ctggagcgaa gggatgggga tcacattcat tgcagaagac acaacccaaa aggggatgac
      721 gtaactgatg caacgaaaat catttgaaat accaaaagca ctggtctggg catcatatct
      781 ggatgtgcgt cgcaacaagg gagccccccg gatgcgacgg gcagactttg aaaatgttcg
      841 accagcaacg tgatggcaat ctatacaaga tctggaaccg gctatgttcg ggaacatggt
      901 ttccgccacc agtgctggaa aaacggatcc cgaagtcgaa tggcaaggaa cgcattctgg
      961 ggatcccaac agtatccgat cgaattgctc agggagcgat aaaacttttc atggaagaaa
     1021 agcttgaccc gattttccat gctgattcat atggttaccg accaggcaaa tcagctcatg
     1081 acgctctgaa acaatgtgcc atccggtgct ggcgttatag ctggatactg gaagtggaca
     1141 tcagtgcgtt tttcgaccat gtgagacatg atctggtgct caaagctctg gaacaccacg
     1201 ggatgcctaa atgggtcatc ctgtactgtc gacgctggat ggaagcgcca atgcagagtt
     1261 gtgaaaatgg agaattaata acccgaacac gaggcactcc gcagggcggg gtcattagtc
     1321 cgctacttgc taacttgttc cttcactatg catttgattt gtggatggaa agagaatatc
     1381 ggggggtacc gtttgagagg tacgctgacg atattgtagt acattgttca cgaatgagtg
     1441 atgcaacaag gctaaagaac aggttatcgg agcgcttctc ggaagtcggg ctggtcctga
     1501 atgcagggaa aacgaatatc gcctacattg acacgtttaa aaggagaaac gtcgcaacga
     1561 gtttcacctt tctcggatat gacttcaaag tgcgtacgct gaagaatttc aacggcgaac
     1621 ggtaccgaaa atgcatgccg ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa
     1681 tcaagaagtg gcgtatacat cgctcaacag ctgagagttt gctggatttt gcgaggcgct
     1741 acaatgcgat agtgagaggc tggatcgagt actacggaaa gttctggtcc agaaatttca
     1801 actatcgact gtggagtgca atgcagtcac gtctgctcaa gtggatgcag tctaaataca
     1861 gactttcgaa ccggaaggct cagcgaaagc tgacgctggt aaggaaggag tatccgaagc
     1921 tatttgttca ctggtattta ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg
     1981 agagtgtcac gtacggttcc gtgagcagcc ggggagtgaa attctctccg gctgactcga
     2041 ctcgcgggtt ccgacagcga tgacgaacat gcggcggtgt tgtactcgct gatcggcaca
     2101 tgccgtctga acaatgtgga accagaaaaa tggctgcgtt acgtcattga gcatatccag
     2161 gactggccgg caaaccgggt acgtgatctg ttgccctgga aagttgatct gagctctcag
     2221 taaatatcaa tacggttctg acgagccgct tacttttggc gtaattctgc tgcgccttta
     2281 ctgaacacaa tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt
     2341 tccattgaaa ccaaccgacg catattcaaa aaagtctgga cgtcctgttg ctccagagtt
     2401 tttattggta cgggtcgtat agttccatgt tggcaggcct cagctattgc ctgtgcgtcg
     2461 ttataatcat ttttctggca ccgctgataa gctttaacat gttgtgccgg aagcaatatt
     2521 acatcatggt cgagtttcct gatttcccgg gcccagtaat gagatgcccc gccagcctcc
     2581 atcgcaacag tcattttagg ctgttgcacc agccagttaa gcagttgatt gcgagaaagt
     2641 ttggtctttc ctgcgggttt gccatgagca ttgatactga aaagataaaa gttcgtttta
     2701 gccagatcaa tgccggtgat tttctgttca gacatggtat cccgcttact tttgttgtta
     2761 aagtttatcc atcatggcgc aattgaaggc tgccgtgaaa gagggggaag actatctcat
     2821 cggttactgt taaacggcta agagaaacta tctttttcaa gcaaagaaga tacggtgaaa
     2881 caggcgattt caccgtgaaa tactgttcaa aaaaaacgct aataaaagca aaatttcttt
     2941 taaaacagcc aattaaaaac tggcgagttg ctggcgaaaa acttgaattt tcacagcgaa
     3001 ttcagattat cttttagtac aagagcaatc atacgatcta acttatcctg aacttccaac
     3061 ggtaatctgc cgaattcata tgagaacgta cgtcccttta ctcgttttcg agcaaattta
     3121 tctttactat caaatttcca taacaaagtt acaaccgctt tatcctttac tcctgaatca
     3181 agcaatgaag acatttcctt ggttataagc cgcataatac tgtttttaac ctcgtcttca
     3241 gaaagattgt catcagcagt aataagctca atactcggcg agatattctt cacaacttca
     3301 tcaatagata tatcacccag cctcagtcgt tcacttaatt cagccagttg cctgtaatcg
     3361 gcgtatgtca gctcagactg aacaggaaaa agagacacaa aatctttagg tacggacgct
     3421 gcttggaggg cacgagtcac cttagctgcc gacaatccct ctctttccgc aatttgcttc
     3481 tggttcatac cggcctcttt gagacgtact aaacgcaacc caatttcacg aatattatgt
     3541 tcaagtgacg tctgcaaatc tttcgccaga tgctgagctt cagaaacagt aagctcatca
     3601 tccgttacaa gtacacgtaa tccaaccttg catagcaaag ctgctgcccg acgacgggaa
     3661 ccatccaaaa tctcaataag atcgccagtc cgaatcccaa tacatggata gaattgttgt
     3721 aaacgtatag ttctggtgat gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg
     3781 ccattcacct cttcaaccac aaaagtttga gtatccactt tgtcatgatc aaccttgatc
     3841 tcagtaaatt ttgcctttcg gccagtattg agggtaaata catgaactgg agccgatatt
     3901 tcttcagtat tattcaggat atttgtattc agcgtacggc caatagtcgg tcggtgcttt
     3961 ctgttttcca tcagtagttg gcccttataa attcaatacg atcaaaaact gctttagcga
     4021 aatcttctgc tgccatacgt gcatttttca aagcttcacc actgccaaca taagtaacag
     4081 gattcgcaga tatcacggta tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg
     4141 gcattgaaac atcaagcatg tcaccaccga aaatctcttt tgtcaggcta tggcagtatt
     4201 tatgatctga cttattcgcc agtttcgaca tgaagccaat atttgcctgc aaacggcatg
     4261 aacatccatc ctgttcaata atctgtacaa gttcaggtaa acgagccaaa tatttaagcg
     4321 tggaatggaa atcaacttgt gctggcggca ccggtgtaaa catgatatct gcggcagcaa
     4381 tggcattttt caaaaatgcg tccagatgcg ggcctgtatc aataaggatg aagtcgaaat
     4441 catgcttcag cttatcaata atattctcac gcaatatcgc gtgtttgttt tgtcctaata
     4501 gatgctcctc gcacagagta tcccagtttg atgcaataaa ggcgtcgtca atagaagccg
     4561 gcataacata aacgccaggg ataactgacg gaacgataaa atcttccagt aattcctcac
     4621 gactcacgtt ctgcaacata gcctgcggtg cggttgtatc aaccagcccc accgcatgca
     4681 agtagttcag aaacatagtg gctgatgact ggggatctaa atcaaggagc agaatacgta
     4741 aatcttctga cagcaggtga ggatgagcac gcagggcgtg agctacggaa acactactca
     4801 cggttttaga cacaccgcct tttagactac cgatgaatat ggaatatgcc tcactataac
     4861 gatcgcgata tttaggaatg ccacgatgtg catagatatc gataatgttc tggatggtta
     4921 atgcatattt tgtcgcggtt cctgctgggc gtttttcaaa ctgatagccc tgagcctcca
     4981 tctcaccgac tgcaccatcc acgcttgcgc gcgttaattt tggaagctta gctacagcag
     5041 actttgaata gacttgataa aaaacatcag cctgaaactc ctgcttttgc tcctgaatct
     5101 gttttgttag ggcgagcaac attttatctg cacgctgcgc aaccttactt aactgctcaa
     5161 aactagtcat tccccctccg tgctatgtag gattttaact tattacaaaa aaaactgcat
     5221 tagcagggta atgcatgcaa gtacatattg tacatgctta tattaaaaat gcgcgcattg
     5281 ctgatttatt aatcatcgga atgaatatta ctgtaagata gtatgccttt ccgggagaaa
     5341 gacgctgatt tcccccgaat aatccagttc accaccacac tccggacaac agctttcttc
     5401 aggctccaga tggtgtattt cgcgggaaac atgttccggt aacggacggc gatgacgtga
     5461 ctgtcgcaac aggcgggggt agtgcttcca atcagtagaa catgtgcttt tcaatgaagg
     5521 agccgatgac tttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca
     5581 gccgcttgat gcgggtgcgg agggtccggt tgttacgtgc aatacgttgc gtgaagattt
     5641 tgccggtcag gtgtttctcc ttcggcactt ctctggcgta gctgccccag tcatcgctgg
     5701 tcaccatgcc gatacagaac ggagtcaaca gggctaacag ttcccgacag gtctcatcat
     5761 tccgtggccc gaaagtgtaa gccaacacac cgccagtttt agtgttgtac gcataccaga
     5821 gccagtgttg tgtcaacgac ggatgaaaag tgatccactt atatctccac caacggccca
     5881 atattgatcc accgttttac tcaggattag cttctgctat aaccccggcc tttcgtttct
     5941 gtctgagtcg atagctttct cctttgattt gaacgacatg tgagtggtgt aagatacggc
     6001 ccagcatcgc tgaggtcagt gctgcatcac cggcgaacgt ttgatcccac tgcccgaacg
     6061 gtagattgga tgtcaggatc attgcgctct tttcgtaacg tttagcgatg acctggaaga
     6121 acagttttgc ttcttcctga ctgaacggca gatagcctat ttcatcaatg atgagcaggc
     6181 ggggggccat tactccacgc tgaagcgtcg ttttataacg gccctgacgt tgtgccgtag
     6241 ataactgaag taacagatct gctgctgttg tgaagcgaac tttgatacct acacggactg
     6301 cttcatagcc catcgctatt gccagatg
//
LOCUS       DAJVQR010000164         6260 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVQR010000164 DAJVQR010000000
VERSION     DAJVQR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OQM52_003850"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OQM52_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1565508.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OQM52_003851"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OQM52_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1565509.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OQM52_003852"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OQM52_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OQM52_003853"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OQM52_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1663 a         1455 c         1471 g         1671 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgttaa ttaacaaatg atatcttcta tatattttta
     6181 ttgtttatag atggctattt cggtaaaaat gcattttcag aatatcaacc agcggtaaat
     6241 cgaagtaaag ggagtgaatt
//
LOCUS       DAJVQR010000165         6055 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVQR010000165 DAJVQR010000000
VERSION     DAJVQR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            433..1341
                     /locus_tag="OQM52_003854"
     CDS             433..1341
                     /locus_tag="OQM52_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCS1565510.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OQM52_003855"
     CDS             complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OQM52_003855"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1565511.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3656..4414
                     /gene="loiP"
                     /locus_tag="OQM52_003856"
                     /gene_synonym="yggG"
     CDS             3656..4414
                     /gene="loiP"
                     /locus_tag="OQM52_003856"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1565512.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4620..5540)
                     /gene="speB"
                     /locus_tag="OQM52_003857"
     CDS             complement(4620..5540)
                     /gene="speB"
                     /locus_tag="OQM52_003857"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1565513.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5676..>6055)
                     /locus_tag="OQM52_003858"
     CDS             complement(5676..>6055)
                     /locus_tag="OQM52_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565514.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1562 g         1426 t
ORIGIN      
        1 ttgctgaatg tataactgag attgatgatt ctggaaaata atcactaagc cgcataaatt
       61 atccttattt tatttttagg gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta
      121 atgaaagcaa ttgggtacgc gagaagaaaa accccacaaa ccaaaataat aaaaccttaa
      181 ataacaacaa tatgaaattg atatttttaa agtcaacttt gtatttgtta tgttcatcac
      241 aaaatcaaca ttttgtttgt tattgtattt gtgatgcata tctctattta tttattgaat
      301 gttcgaaaac atacttttct aatgcaatgt tttttcgcta taccccttta tagattctga
      361 attctcacct gtcggctgtt ttctcctcat ctcatgtgac atattcccat actttactca
      421 aggaagattg tcatgaaagc acctgtttca ttaacctcat ttcgcccaca aaaatctctt
      481 gctatcgcca ttggcgtgct tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt
      541 aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg
      601 gggctgggtt ttaagatccc ttttatggaa agcgtggaga aaatttccac gcgtaatcag
      661 gctgtggtct atcagggatt acaggcctac agccgcgacc agcaaccggc acaaatgaca
      721 gtatcagtca gcttccacat taaaccttca gaagcaggcg cggtttatac cacctacaac
      781 actattgaag cattaaaaga tcgtctaatt gtccgtcagt tgccaactca gctggagaac
      841 attttcggcc aatataccgc catcagcgcc gtacaggatc gtacaaaatt ggttcaggat
      901 ctgcaaaatg ccatgcgcaa agccgttgtt gggccagtgg taattgacgg ggtccagatt
      961 gaaaatattg atttttctga cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa
     1021 gtcgccattg ccacgcgtaa gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg
     1081 gtaactcagg cccaggcaga agcagacagt aaactggcgg cagccaaagc agaagcggaa
     1141 actattcgcg taagaggcgc ggcagaggct gaaactattc gtttaaaaag tgcggctgaa
     1201 gcagaagcaa tccgcctgcg cggtgaagct ctgcgggata atcccggact tgtagccctc
     1261 accactgccg aacgttggga cggtaaactg cccgatacca tgatccccgg cagcacagtt
     1321 ccgtttatct ctactaagta aaaaaagggc cgctttcgcg gtccttctca caaaacgtct
     1381 tactgattac agcagcgctt ttgctttcgc aacaacgtta tcaacagtga agccgaactc
     1441 ttcaaacagc agctctgccg gagcagattc accgaaggtg gtcatgccga cgatagcgcc
     1501 gttcaggcca acatacttgt accagtagtc agcaataccc gcttctaccg caacgcgtgc
     1561 agtaaccgct ttcggcagta cggattcacg gtaagccgca tcctgcttgt caaatgcgtc
     1621 ggtagacggc atggacacca cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc
     1681 agcaaccgcc agttcaactt cagaaccggt ggcgatgaag atcagttccg gctgaccagc
     1741 acagtctttc agcacataac caccgcgcgc gatgtttgcc agctgctctt cagtacgttc
     1801 ctgctgcgcc aggttctgac gggagaggat aagcgcagtc gggccgtcct gacgctcaac
     1861 gccgtatttc cacgcgaccg cggattcaac ctggtcacac ggacgccatg tagacatgtt
     1921 cggggtcacg cgcagagaag cgacctgctc taccggctgg tgagtcgggc catcttcgcc
     1981 cagaccgatg gagtcgtggg tgtaaaccat cacctggcgc tgtttcatca gcgcagccat
     2041 acgtacggcg ttacgtgcgt attccacgaa catcaggaag gtggaggtgt acggcaggaa
     2101 gccaccgtgc agggagatac cgttagcaat cgcggtcata ccgaactcgc gaacaccgta
     2161 gtggatgtag ttacctgcag catcttcgtt gattgcttta gaaccagacc acagggtcag
     2221 gttagacggt gccaggtcag cagagccgcc gaggaattca ggcaacagcg ggccgaacgc
     2281 ttcgatagca ttctgcgacg ctttacggct ggcgattttc gccggattag cctgcagttt
     2341 agcgataaac tctttcgctt tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg
     2401 ggtaaattca gccgcttcct gcggataagc tttcgcgtaa gccgcaaact tctcattcca
     2461 tgcagattct ttcgcctggc ctgcttcttt cgcatcccac tgagcataga tttcagacgg
     2521 gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc
     2581 gcccagcggc gcaccgtggg agtcgtgagt accggctttg ttcggggaac cgaaaccgat
     2641 gatggttttg cacatcagca gggacggttt gtcagtcact gcgcgcgctt cttcaactgc
     2701 gcgtttgatg gatgccgcgt catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc
     2761 ttcgaaacgc attgctgtgt cgtcggtgaa ccagccttca acgtgaccat cgatagaaat
     2821 accgttgtca tcgtagaacg caatcagttt acccagcttc agcgtacccg ccagagagca
     2881 aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg
     2941 gtcgacaatg tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc
     3001 cataccgact gcgttggcaa taccctgacc cagcggaccg gtggtggttt ccacaccagc
     3061 ggtataacct acttccggat ggcccggagt tttagagtgc agctgacgga agtttttcag
     3121 ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca gcatggagcc
     3181 gtggccgttg gacagcacga agcggtcacg gtcagcccag gacggattct gcgggttgtg
     3241 tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc atacccatag gggcacccgg
     3301 gtgaccggat ttggctttct gtactgcgtc catgctcagc gcacgaatag cattggcaag
     3361 ctctttacgt gaggacattt tgactccaga tcggatgatg aagggcacgc ccttaacgac
     3421 ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc aacaatttac cgcaagccgc
     3481 gcgtcatgta catggaacat ccttttgccg cttcagaaat ctctggatca tgctcgcatg
     3541 ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat actgagactg agcgtcgatt
     3601 cacctgcaaa cggcgcattt ttagaataat cctgaccttg tgcggaagag aaaacatgaa
     3661 aattcgcgcc ttattggtag caatgagcgt ggcaacggta ctgaccggtt gccagaatat
     3721 ggactccaac ggactgctct catcaggagc ggaagctttt caggcttaca gtttgagtga
     3781 tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag atggacagca aggcgacgat
     3841 tgcgccagcc aatagcgaat acgctaaacg tctgacaact attgccaatg cgctaggcaa
     3901 caatatcaac ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt
     3961 tgcaatggct aacggctgta tccgcgtcta tagcgggctg atggatatga tgacagataa
     4021 cgaagtcgaa gcggtgatcg gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa
     4081 agggatgcag gtggcgcttg gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg
     4141 gattgtcggc agtttatctc aatcacaact tggtgatctg ggcgagaaat tagtcaattc
     4201 gcaattctcc cagcgccagg aagcagaagc cgatgattat tcttacgatc ttctgcacca
     4261 acgcggcatc agcccggcag gtcttgccac cagctttgaa aaactggcga agctggaaga
     4321 aggtcgtcaa agctcaatgt ttgacgacca tcctgcatcc gccgaacgtg cccagcatat
     4381 tcgcgatcgc atcagcgcgg atgggattaa gtaaagccta gtggtgtcga acgtaggtca
     4441 gataaggcgt tcacgccgca tctgacatta atggcacatt tgttaccttg tgcgcattgc
     4501 cggatgcgat gctggcgcat cttatccggc ctacgggtgc cgaacgtagg tcggataagg
     4561 cgttcacgcc gcatccgaca ttaatggcac gttttacccg tgcgcatcgc atctggtgct
     4621 tactcgccct ttttcgccgc ctgaatatac agcatctcca gcgccagcgt cgccgcagcc
     4681 agagcggtga tttccgactg atcgtatgcc ggagccactt ccactacgtc catcccaacg
     4741 atgttgagat ctttcaggcc gcgtaccagt ttaatggcgc gatcggaggt caggccgcca
     4801 atcactggcg taccggttcc cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc
     4861 aggtaaaccg gcatatcacc cacaatctgt ttcacctggg cgataacgtc atccacgctg
     4921 cgatcgttca cctggcaggc gtccagcacg gtaaagccgt tgtctttatc aaactcggta
     4981 cgaataccaa tctgcacgga atgattcggg tcgatcagac cttctttcgg cgcggtatag
     5041 aacatagtgc cgtggtcaaa ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag
     5101 tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca
     5161 aagtggtcac caccgaagga gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc
     5221 gcctgcagct tttcgctcat ctcacgggca tcgccaaagg catataccag atcgccgcag
     5281 tccacgacgt tcagacgctc acgcatgtcg aaattccacg ggaagcggtt gtgttcccag
     5341 gccagattcg tcgaaacctg acggatcgct gccggaccgt ggcgaccacc cgcacgacca
     5401 gaagtggcca tatcgaacgg cacgccagta atcacccagt ctgcatcgct gtcatacggc
     5461 tggaagttca tcggcaggcg taaaaaacca aaggcattgg aaaccagtga gttatcgtat
     5521 tgatgaccta aggtgctcat ggatggactc ctattacaaa gtcgatataa aaaaaccctt
     5581 ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga aacacaagtt tgtgctaatt
     5641 atcgccttaa atgggcgagg catcaagcga ggttgctatt tccgacacgg tggcggcgca
     5701 tagccattgt tatctgccgg aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc
     5761 gggccttgta attcaaaggc ggcaaagccg attaccccgg tattacgcac atcgccttgc
     5821 gccgaattgg ctgcgtagga atcagcaacg ttggcgaact gaaaagccgc ttcagtatgt
     5881 ttatctttgc ggaatccttt aatcactaac gaatctccgg cattcacaat atagccgttg
     5941 ttattcagcg aaccttgctt gccgttcagc acatccaggc catcagccgt agcaacgatc
     6001 tcgtagttgg tgttacgact gtaattacgg acatataatt gataattcat tccga
//
LOCUS       DAJVQR010000166         5983 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVQR010000166 DAJVQR010000000
VERSION     DAJVQR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(1..393)
                     /locus_tag="OQM52_003859"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OQM52_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OQM52_003860"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OQM52_003860"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCS1565515.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OQM52_003861"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OQM52_003861"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCS1565516.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="OQM52_003862"
     CDS             5797..>5983
                     /locus_tag="OQM52_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565517.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJVQR010000167         5951 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVQR010000167 DAJVQR010000000
VERSION     DAJVQR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..678
                     /locus_tag="OQM52_003863"
     CDS             <1..678
                     /locus_tag="OQM52_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1565518.1"
                     /translation="EVRDVWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQV
                     YQDELKPVHPMLEALRSGIPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKAL
                     QELDTEDQRHLYLMLKEDYDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVV
                     SELEKPSKSKKVSAIKVVKPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMA
                     SLFKQAG"
     gene            681..2207
                     /locus_tag="OQM52_003864"
     CDS             681..2207
                     /locus_tag="OQM52_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS1565519.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            2208..3824
                     /locus_tag="OQM52_003865"
     CDS             2208..3824
                     /locus_tag="OQM52_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS1565520.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(3880..4032)
                     /locus_tag="OQM52_003866"
                     /pseudo
     CDS             complement(3880..4032)
                     /locus_tag="OQM52_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4055..4432)
                     /locus_tag="OQM52_003867"
     CDS             complement(4055..4432)
                     /locus_tag="OQM52_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565521.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(4842..5213)
                     /locus_tag="OQM52_003868"
     CDS             complement(4842..5213)
                     /locus_tag="OQM52_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565522.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(5274..5771)
                     /locus_tag="OQM52_003869"
     CDS             complement(5274..5771)
                     /locus_tag="OQM52_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565523.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(5847..>5951)
                     /locus_tag="OQM52_003870"
     CDS             complement(5847..>5951)
                     /locus_tag="OQM52_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'') family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="HCS1565524.1"
                     /translation="QAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1647 a         1249 c         1462 g         1593 t
ORIGIN      
        1 gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt agtaaaactt
       61 tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac cgctggttta
      121 ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga ggcattacgc
      181 tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga gattgataaa
      241 cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc agaagaaaaa
      301 gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct gaaagaggat
      361 tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa tccaacgatg
      421 acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga aacggtagtg
      481 tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt agtcaagccc
      541 agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca acgccaacct
      601 gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat ggcgagctta
      661 tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat gagctgattt
      721 atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag cagctgttgg
      781 atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg catttaggtg
      841 tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt atttatgagc
      901 ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac gaagctattc
      961 ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc gctgcttcta
     1021 gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt cagctaaata
     1081 ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca tattgtccaa
     1141 aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat caattttggg
     1201 ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct caattaacag
     1261 cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag ctttccccaa
     1321 gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg ctaaccaaaa
     1381 gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt agtgatgagg
     1441 cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg ctgaaaagta
     1501 tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg tggcaagcct
     1561 tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt actgagcact
     1621 ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat ttatctgtta
     1681 agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg gcaagacagt
     1741 ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat tggctagttc
     1801 actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt gattggaacc
     1861 aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta gatagtagcc
     1921 ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac ggaacctctc
     1981 ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt tactcagaga
     2041 gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt aaacaggaag
     2101 atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa gagcggataa
     2161 ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg gttaggctag
     2221 ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt aactcgggtc
     2281 ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa tggactcgat
     2341 ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca aagcaaataa
     2401 ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc aaaataatcg
     2461 attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg cagagttcat
     2521 ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca cagttagagc
     2581 tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg agcagtaccg
     2641 ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta gagataagga
     2701 tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta gtgcagcttt
     2761 tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt tttaggcact
     2821 atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc tttgaccctc
     2881 cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag gataaggatt
     2941 atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc acaacagcta
     3001 ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag cacatagcgg
     3061 tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag gaaacagcca
     3121 atatagacac agacacacga gtcatagagg ctgagccgac atggataagt tttagtagac
     3181 ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt ttagaaaaag
     3241 aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca cacttaggtg
     3301 gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac aactctattt
     3361 ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact aaatttgcat
     3421 gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc cgattacatc
     3481 tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt aatggcagtg
     3541 aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct accaaagtgt
     3601 tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg cgtggagtgg
     3661 tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga caagacgggc
     3721 atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga gaggatatgg
     3781 aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat gactgaaaga
     3841 gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc catttaaacg
     3901 gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga tcggcatcgt
     3961 ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg attcgtccgt
     4021 agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg tggttttgag
     4081 gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg gcgacatgtt
     4141 tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt gcttggacga
     4201 ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga aatccaatat
     4261 cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa gcttcccagg
     4321 ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca tcaacttttt
     4381 tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac acgtgcgttt
     4441 cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt cccaaagcat
     4501 ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt gatatttgaa
     4561 acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc ttgtaaatat
     4621 cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta tagaaatcgt
     4681 cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca tcgccatacc
     4741 ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga gcttgtacgc
     4801 aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc catccattcg
     4861 gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta tgttgcctgc
     4921 accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc ccacttgccc
     4981 acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc ctcctttaac
     5041 tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc gacatagtct
     5101 ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt gtcatacatg
     5161 acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga cactagtggt
     5221 tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc aggctagttg
     5281 cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg gccatttatg
     5341 acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg cccccctttt
     5401 ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc attgttggca
     5461 atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag cctacgattt
     5521 ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc agagttgtcg
     5581 taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct tggagaaatg
     5641 tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa aacaattggc
     5701 aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac caccttcaac
     5761 agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc cgcgaagcgg
     5821 cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc
     5881 acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttgtcca
     5941 agataagcct g
//
LOCUS       DAJVQR010000168         5838 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVQR010000168 DAJVQR010000000
VERSION     DAJVQR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            53..571
                     /gene="mddA"
                     /locus_tag="OQM52_003871"
     CDS             53..571
                     /gene="mddA"
                     /locus_tag="OQM52_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1565525.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            568..1017
                     /locus_tag="OQM52_003872"
     CDS             568..1017
                     /locus_tag="OQM52_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1565526.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1018..1251)
                     /locus_tag="OQM52_003873"
     CDS             complement(1018..1251)
                     /locus_tag="OQM52_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCS1565527.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1337..1510)
                     /gene="ortT"
                     /locus_tag="OQM52_003874"
     CDS             complement(1337..1510)
                     /gene="ortT"
                     /locus_tag="OQM52_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1565528.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1705..1800
                     /gene="yncL"
                     /locus_tag="OQM52_003875"
     CDS             1705..1800
                     /gene="yncL"
                     /locus_tag="OQM52_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1565529.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2202..3011)
                     /locus_tag="OQM52_003876"
     CDS             complement(2202..3011)
                     /locus_tag="OQM52_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCS1565530.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3221..4645)
                     /gene="patD"
                     /locus_tag="OQM52_003877"
     CDS             complement(3221..4645)
                     /gene="patD"
                     /locus_tag="OQM52_003877"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCS1565531.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4667..5461)
                     /locus_tag="OQM52_003878"
     CDS             complement(4667..5461)
                     /locus_tag="OQM52_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1565532.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5451..>5838)
                     /locus_tag="OQM52_003879"
     CDS             complement(5451..>5838)
                     /locus_tag="OQM52_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1565533.1"
                     /translation="QTMTSRSRQAAYSMILPVQAALERLPPSLLQASADLGARPRQTF
                     RYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAA
                     AFTLVPIILIALYLAFVKRLGAFDAL"
BASE COUNT         1528 a         1483 c         1298 g         1529 t
ORIGIN      
        1 tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag acatgtccat
       61 ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc acgccgtgtt
      121 gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca ttgcctggtt
      181 tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg gcgtagttac
      241 gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata ccgtggaaca
      301 ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat tgttaagtcg
      361 attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca
      421 aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg cacaaatgcc
      481 gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt tgcaactcga
      541 cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc ttttttgatt
      601 gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac ccagacctcc
      661 tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct ctttgtttct
      721 attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag cgtgcgctgg
      781 tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat cagcggatat
      841 cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct gattggcggt
      901 ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt cggcccaata
      961 tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc attttaatca
     1021 gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt ctttatgctt
     1081 gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg
     1141 acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg caatgacgct
     1201 ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca tactttctcc
     1261 ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt ggtgccgtca
     1321 agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc
     1381 atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa
     1441 accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc atatgttgat
     1501 agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata tgcgccattt
     1561 tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc
     1621 cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact atacacatag
     1681 gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt actgataaat
     1741 gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg gtttatttga
     1801 tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga cagtacgcaa
     1861 gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc tttttgctgg
     1921 catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa acagcttact
     1981 gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata cgcgcgggag
     2041 tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg cgtaaagtat
     2101 atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa ttgcaaaccc
     2161 cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc tctgaatacc
     2221 tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga tatcaatcga
     2281 ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg aagatctgag
     2341 tagttataat tccaccattt tattctcaac agttcatcaa taacatttga atcaaaccta
     2401 aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac atctttggta
     2461 acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc tttcaataca
     2521 acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt ggtaatgaat
     2581 ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa ttcaccgtta
     2641 taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat tatttttaca
     2701 tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg gcaggtcgga
     2761 aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata ttcttcagca
     2821 ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa attcctttct
     2881 ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca aaactcaaag
     2941 tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa tttgtacaat
     3001 tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg atactctaaa
     3061 gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt agcctaattt
     3121 ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact cagaacctaa
     3181 aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta accatgacgt
     3241 ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg taaccagaaa
     3301 gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg acccaggtac
     3361 aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc gtccataccg
     3421 aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct tcgttgtcga
     3481 agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca tcgtcctgta
     3541 atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc ttttcaccgc
     3601 cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg ctgacgcgtt
     3661 cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc ggcgcaccag
     3721 attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa atgccttttt
     3781 gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag ccaaatgtac
     3841 gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact ggcgctttgc
     3901 caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg atgtgctcgc
     3961 cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg gtcagcggat
     4021 cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg aagatatctt
     4081 tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt ttaagcacta
     4141 cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc agcggataat
     4201 tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc gaagtatgac
     4261 cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc gcgaaaaagc
     4321 gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc ggtttgccac
     4381 aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca tccgccagtt
     4441 tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca aatgctgcat
     4501 ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc tccagtaaaa
     4561 cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta acccgttctc
     4621 cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat tgaccattgt
     4681 cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc aacataacca
     4741 gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga ttgagcaacc
     4801 acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt tcatcgaacg
     4861 acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc ggcaacacta
     4921 cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca accagactcc
     4981 acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg caaaaagtcg
     5041 catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta aaggcggtta
     5101 atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat aacagcgaaa
     5161 tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc gtccctaaca
     5221 ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg gcatcaagaa
     5281 tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc ggtggaaaac
     5341 taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta
     5401 ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca
     5461 tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat aatcggcacc
     5521 agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga ataaaccata
     5581 ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc gcccagtgtg
     5641 agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg cagcaccaca
     5701 tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa caatgacggc
     5761 ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc ctgacggctg
     5821 cggcttgtca tggtctgc
//
LOCUS       DAJVQR010000169         5808 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVQR010000169 DAJVQR010000000
VERSION     DAJVQR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..1283
                     /gene="celF"
                     /locus_tag="OQM52_003880"
                     /gene_synonym="chbF"
     CDS             <1..1283
                     /gene="celF"
                     /locus_tag="OQM52_003880"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1565534.1"
                     /translation="IKRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMIDNAGVPMKL
                     YKTLDCREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTI
                     PVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKMFIHD
                     VLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKVSGVKNIFDLP
                     FSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNP
                     ELKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMT
                     CKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLVH
                     SDRDAELLAREMILAHEKWLPNFADCIAELKKAH"
     gene            1296..2054
                     /gene="chbG"
                     /locus_tag="OQM52_003881"
     CDS             1296..2054
                     /gene="chbG"
                     /locus_tag="OQM52_003881"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS1565535.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2101..4362)
                     /gene="katE"
                     /locus_tag="OQM52_003882"
     CDS             complement(2101..4362)
                     /gene="katE"
                     /locus_tag="OQM52_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS1565536.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4545..4808
                     /gene="cedA"
                     /locus_tag="OQM52_003883"
     CDS             4545..4808
                     /gene="cedA"
                     /locus_tag="OQM52_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS1565537.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5085..5808
                     /locus_tag="OQM52_003884"
                     /pseudo
     CDS             5085..5808
                     /locus_tag="OQM52_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1471 a         1252 c         1478 g         1607 t
ORIGIN      
        1 ttattaagcg ttatcacgaa ttgccggtca gcgaattatg gctggtggat gtcgagggtg
       61 gcaaagctaa actggatatt attttcgatc tctgccaacg gatgattgat aacgctggcg
      121 tcccgatgaa gctttataaa acgctggatt gccgcgaagc attgaaagat gctgatttcg
      181 ttactaccca actgcgcgtt ggccaattac cggcgcgtga actggatgaa cgtattccat
      241 taagtcatgg ttatcttggt caggaaacca acggcgcggg cggtttgttt aaaggtctgc
      301 gtaccattcc ggtgattttt gacatcgtaa aagatgtcga agaactttgt ccgaatgcat
      361 gggtgattaa cttcactaac ccggcgggaa tggttactga agccgtttat cgtcataccg
      421 gatttaaacg ctttatcggc gtgtgtaata ttccgatcgg catgaagatg tttattcacg
      481 atgttctgat gctgaaagac agcgatgatt tatctatcga tttgtttggc ctcaaccata
      541 tggtgttcat taaggatgtg ctggtaaatg gcaagtcgcg ctttgccgaa ttgcttgatg
      601 gtgtggcatc cgggcagtta aaagtatctg gcgttaaaaa tattttcgat ctgccattta
      661 gcgaaggctt aattcgttct ctgaatctgt tgccgtgttc ttatttgctt tattacttca
      721 agcaaaaaga gatgctggct attgaaatgg gcgaatacta caaaggcggc gcacgagcgc
      781 aggtagtaca gaaagtcgag aaacaacttt ttgagctgta taaaaacccg gagttgaaag
      841 ttaagccgaa agaactggaa cagcgcggtg gggcttatta ctctgatgca gcgtgcgaag
      901 tgatcaacgc tatctacaac gacaagcaag ctgaacatta cgttaatatc ccgcatcatg
      961 ggcatattga taatattccg gcagactggg cggtagaaat gacctgtaag ctggggcgcg
     1021 atggcgcgac gccacatccg cgcattacgc atttcgatga taaagtgatg gggctgattc
     1081 acaccattaa aggcttcgag attgctgcca gcaacgccgc acttagcgga gaatttaacg
     1141 atgtgttact ggcgctaaac cttagtccgt tggtgcattc cgatcgcgat gctgagctgc
     1201 tggcacgcga gatgattctg gcgcacgaga aatggctgcc aaattttgcc gactgcatcg
     1261 cagagcttaa aaaagcacat taaccgaggc tgattatgga acgcttactg attgttaatg
     1321 ccgatgattt tggcttaagc aaaggtcaga actacggcat tatcgaggcc tgtcgcaatg
     1381 ggattgtcac atcgacgacg gcgctggtga atgggcaggc tattgaccat gcggtgcagt
     1441 tgagtcgtga tgaaccgagt ctggccatag ggatgcactt tgtccttact atgggcaagc
     1501 cactgacagc tatgccgggg ttaacccgcg atggtgtgct gggaaaatgg atctggcagt
     1561 tggcagaaga agatgcttta ccgctggaag aaattactca ggagcttgcc agtcagtatt
     1621 tgcgtttcat tgagctattt ggacgcaaac ctacgcatct tgatagccat catcatgtgc
     1681 atatgttccc gcagattttc ccgattgttg ccaggtttgc ggctgaagag gggattgcgt
     1741 tgcgcatcga ccgtcagccg ctatctaacg ctggtgattt accggctaat ctgcgcagtt
     1801 cgcagggatt cagtagcgcg ttttatggtg aagagattag cgaggcactg ttcctgcaag
     1861 tgctggatga cgcaagccat cggggtgatc cttcgctgga ggtgatgtgt catccggcgt
     1921 ttatcgataa tactatccgt cagagtgctt actgtttccc acggttaacg gagctggaag
     1981 tgctgacatc agcgtcgttg aaatatgcaa ttgctgagcg tggttatcgg ttgggtagtt
     2041 atcttgatgt gtaagtaagg tggtaaggtc actccccagg ggacgtgacc ttatccatat
     2101 ttacgccgga attttgtcaa tcttaggaat gcgtgaccac acgcggtgtt ctgccatcag
     2161 cgttaacagt tcatccataa aactaccgtc ggtgctgtca gcttccacaa tcccttcttc
     2221 accctggtca gctaccttga ttgttgcttt aaacttgcgc gcgtctcccg ccagcgcaat
     2281 cggtttaagg tgtttgtagg cttccatcag atagtagttg gcatcgccgt tgtcagcgat
     2341 atccgcgata ttgccgcaag ggacaatgac cgcatcgacc gtcagcgaag gtgcaccggc
     2401 aaaggtagcg gctataggca acaccgtacc gtcatccgca gtcacttcac ccattcggga
     2461 gtagagcagt ttggcatgaa cgcctttggc cttcatggcc ttgagaatgg ccagaaggtc
     2521 tgccgatctc acttcatcat taagtaaaat cgctaccacg cgacctttca catcaccgtc
     2581 aggaatggcg tacaaactta aggatggatc ctttttcaga ccgttgacgt ccggaggtgg
     2641 ggtgatattc agctggtcgt cagtcagttc gataccgaga tttttcgcca ccgcctgggc
     2701 cagagtgaga tcaatatgcg ccagctggtc aacaacgcgc tcacgaatat acggacgaac
     2761 gactttgctt aactcaaaac tgaaaccatc gacaatatgg cgctgctcga atggcgtctg
     2821 acttagccag aacagacgcg gatgggaata atattcgcca aacgatgggc tgcgctcgcg
     2881 aactttattg ccttccacgc gctcttggta tgattcaaaa ccgccgcgtt tcggccccgg
     2941 tggtgtttcg cgcggccagt tatcgttgat cgagttcggt tcataattcg ccgggttagt
     3001 gtcgatcccc atacgatgca tgccgtcacg ctggaaatta tggtaagggc aggtcgggcg
     3061 gttaatcgga atctcatgga aattcggccc accaagacga ctgatttgtg tatcggtata
     3121 ggagaacaaa cgtccctgca acaggggatc gttggtgaag tccagaccag gaacaatatg
     3181 acctggatgg aatgctgctt gttcgttttc ggcaaagaag ttatccggat tgcgattgag
     3241 caccattttg ccgacacgct gaacgggcac cagttcttcc gggatcagtt tggttggatc
     3301 gagaagatcg aagtcgaact tgaattcatc ttcttcagga atcaactgga agcccagttc
     3361 gtattccgga aaatcgcctg cttcaatggc ttcccacaac tcgcggcggt ggaagtccgg
     3421 gtcacgtccg gtgagttttt gtgcttcatc ccaaacgagt gaggctttac ctgccagtgg
     3481 tttccagtgg aaacgtacaa acgttgcctt cccttcggca ttaatcaggc ggaaggtgtg
     3541 aataccgaag ccttccatgg tgcggtaact gcgggggatg ccgcgatccg acatcgccca
     3601 catcacgttg tgcagagttt caggttgcag agaaacataa tcccagaaag tatcgtgggc
     3661 actttgccct tgtggaattg cccagtgcgg ttctggtttt accgcatgaa caaaatcggg
     3721 gaatttatgc gcatcctgga taaagaagat tggcgtgtta ttgccaacga ggtcaaaaat
     3781 accctcttca gtatagaact tggtggcaaa accacggata tcacgcacgg tatcggcaga
     3841 gccagcgcca ccctgaacgg tagagaacct aacaaatact ggggtgattt tgttcggatc
     3901 tgagaggaaa tccgctttgg tgatatcgct taagctttta tatggctgga aataaccgtg
     3961 agctgctgag ccgcgtgcat gaacaatacg ttccgggatg cgctcatggt caaagtgggt
     4021 gattttctcg cgcagaataa aatcttccag cagcgttgga ccacggttac cggcacgcag
     4081 tgagttttga tcatcggcga tgcgcacgcc ctgattagtg gtcagcgcat aattttcact
     4141 gcctttgcgt acgtcttcca gagaattaag tttttcgtta cgtgtatcag gggctttcag
     4201 gctcccaggg gcggtaggtt gtgcacctgg cggtgttggt tcagccgctg gacgatgaga
     4261 tccgtcctca ggtgccagtg agtccatccc cggtttcgct tcgctggaat cgtgtagtgg
     4321 tgactggtgc tgatgtgggt tcttttcgtt atgttgcgac attgaactcg tctccttaat
     4381 ttattactga aagggccgct gttttggtca aaaaactctc taactataga ccaccagcca
     4441 gatcccgctt cggagactat tctggaaacg gtgttatgaa atacacgcaa agcgggacgt
     4501 acaggggcta aatcggctaa actacagttt ttctaatttt taatatgcgt ttagtgaagc
     4561 cagtaatgaa gaaaccgctc cgccagcaaa accgccagat tattagctat gtcccacgca
     4621 cggaacccgc gccgccagaa catgcgataa agatggattc gtttcgtgat gtctggatgc
     4681 tgcgtggcaa atatgttgcg tttgtactga tgggagagtc atttctgcgc tcaccggcgt
     4741 ttactgtgcc tgaatctgcc caacgttggg caaatcagat ccgccaggaa ggggaagtga
     4801 ctgagtaagg attacatacc tcccggtttt gccgggagtt acaggacctg aaattctgca
     4861 tttcgttgct acattcattc aaaccacctg taacaaaaag ctgcctttga cgagctattg
     4921 ctataaataa gagatctttt ttctttaatt atttatttat tttaaaatgg ttaagttttg
     4981 ctaatcatga tgagttattt ttgatagttc atcctgtggc gtcgctctta aacttatatt
     5041 gtaaatgact tagatttaaa taagataata attttgttaa caatatgagg cgggagatgt
     5101 tttctacaaa cttaatacag agtaattatg gtgatttaaa tattaaaagc ctggctttcg
     5161 actcttttaa agaaaggctt gagtctacca tggcggcact cactttcttt atatcaacgg
     5221 gtcaatgtga ctgcgatgaa atagcagagt cgaacttcaa tcgtatgatt gcctatatgt
     5281 ctaacattaa ctatgatgcc tcaaagctag gagcgccagc acttagtttt gatacgtatc
     5341 tgcaggacaa tgttaaatat agagtgataa ttaataatct ttatggcagt gagtttcgga
     5401 taagagatat taatgagaat tttgttggga tggattttac ctgtgaattc cgacccgaaa
     5461 aaatgacgaa tttgatcaat ataaaaaata ggttggttat tcattattta aggactatat
     5521 actatgaaaa aaattatata catcctgccg ggagcatttt tgctgcgatt caaaaaaaat
     5581 gagagtctcc taaagtttcc ttcgataata tcaatgctta atatcaatct tctttttaat
     5641 ccactaaatc ttccgggaat gggaagtggt gtgcttgaag atattatgag tattccagat
     5701 agttcactta gaaaacgtct cgggtatgag gttttaagct tctcactaca ggcacattca
     5761 ttgagccagg aatgtattga taagttggac atttttttcg ctgacgat
//
LOCUS       DAJVQR010000170         5772 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVQR010000170 DAJVQR010000000
VERSION     DAJVQR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(139..603)
                     /locus_tag="OQM52_003885"
     CDS             complement(139..603)
                     /locus_tag="OQM52_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1565538.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(600..1475)
                     /gene="nanK"
                     /locus_tag="OQM52_003886"
     CDS             complement(600..1475)
                     /gene="nanK"
                     /locus_tag="OQM52_003886"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1565539.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1472..2161)
                     /locus_tag="OQM52_003887"
     CDS             complement(1472..2161)
                     /locus_tag="OQM52_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1565540.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2209..3699)
                     /gene="nanT"
                     /locus_tag="OQM52_003888"
     CDS             complement(2209..3699)
                     /gene="nanT"
                     /locus_tag="OQM52_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1565541.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3809..4702)
                     /gene="nanA"
                     /locus_tag="OQM52_003889"
     CDS             complement(3809..4702)
                     /gene="nanA"
                     /locus_tag="OQM52_003889"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1565542.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(4824..5615)
                     /gene="nanR"
                     /locus_tag="OQM52_003890"
     CDS             complement(4824..5615)
                     /gene="nanR"
                     /locus_tag="OQM52_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1565543.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1417 a         1601 c         1473 g         1281 t
ORIGIN      
        1 attaagcgga caggtatata cagtatcaag ggtgttaaca taaaagaata caacgtcagg
       61 taatggcaca gagcggtccg tcagttcagg tttggctctg tgccaaagat gggtcaggtc
      121 acacctgatc taataaattt aagccattaa atcagcctta accttcacca ccaccttttt
      181 aatctcaccc ggctcgccaa caacgcatcc cggcttatgc ggctctcctg gcataaacac
      241 ggcgaacatt cccggtttca gaatgatggt ttgctcgttt tcaatggcgc tacaaagctg
      301 gtaatcatct tcatggtgga actcttcaca ttggcgcgca gtgcctgcca tgccaaacag
      361 aatccgttcc tcaccgttta ataacagctg gatatcaatg tattgctcgt gcaattccgc
      421 ttttttctcg acgggcgatt gagtgttaaa cgtcatgaca ttcataaaaa tattgtcgcc
      481 ctgtaattcg taacgacccg gcgctttttc ttgcggtctg gcagctaagg ccagcgttaa
      541 cgcgtcctgt aacgcaggat gtaacccagc agacggtaat gactgtactt cacccatcat
      601 cataattttt ctccctgggc caacagcgca gccccaagta aacctgcatc atggcggtaa
      661 tgcgccgcca gtaaatcaac atgaaatgcc gctggctcct gcgccagata catttccacc
      721 aacgccagat acccttctgc cagaccaacg ctgccaccaa ccaccacgca ctggcaatca
      781 gttgtggctt taatatcagc gatcagcctt gcaagcgtac gtgcggagcg atgaatcagt
      841 tgctgcgcct gctcatcgcc ctgcccggcg cgtgtgaaaa tagttctcgc atccgcgcca
      901 gccagctcgc cctgcgctgc cgccgcaatg ccgcgaccag aagcaattgc ttccacgcaa
      961 cctgtgcgtc cacagccgca gactgggccg tgtggatcgg caagcgtatg cccgatgtgc
     1021 cccgccagac cgccagggcc ggtaagcagt ttgccgccgc tcactacacc gccgccgacg
     1081 ccagtggaaa cggtgataaa gaccatatcg gttatatcgc cttccagcgc ctgatactct
     1141 gcccatgctg cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt aagttgttcc
     1201 agcgttttga ctaacggaaa gtgtagcaat ccaccgagat tatgcggatt cagcgccagc
     1261 aaactgccgt cgcgaataat tccggttgaa gcgatggcaa cccgctgcgc atgagcttgc
     1321 aacggagaga ctaatgcgga taaggcatca cgcaaggctt ctggtgtctg gctggctggc
     1381 gtaggaagtt cacgacgatc gcggatctgc ccgtcagcgc caatcagcgc ggcggcaagt
     1441 ttagtaccgc cgatatcaat cgccagtatg gtcatagcac cgcctttttc atcgctgtgt
     1501 tgtaccactg acaaatgtgt tcaagacgcg taatggcaga accgaccgtc accgcccacg
     1561 cgccgtggcg catcgcatct gccgcctgag caggcgtgtt gtaacgccct tcggcaatca
     1621 cccgacatcc ggcttcgctc aacgttttca ccagcgccag atccggctct tctggcgttt
     1681 caggcgtggt atagccagaa agcgtagtgc caataatttc ggctcccagc ttttggcatg
     1741 ccaggccgtc ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga tgatggatac
     1801 gtgccagcag cgtttcaaca ggcaccggac gcgggcggtc ggtgccgtca atggcgataa
     1861 tgtccgcgcc cgcctgcgcc agcgcatcga catcttcaat ataggccgtg atgcgtaccg
     1921 gagaatcctc cagatcgcgt ttcacaattc caataatcgg cacgctcacc accgcacgcg
     1981 tggcttgcag atttgccaca ccttcgatgc gaatggcaac cgcgcccgcc tgctctgccg
     2041 ctaatgccat ggctgcgacg atttcgggtt tatcgagcgg gctgtcagga accggctggc
     2101 aggagacaat caggccaccg ttagcagcga ttttttgatc cagttgtgca agtaacgaca
     2161 tacatcttcc cttagcgaaa ggcccggtac atagaccggg caacaggatt aacttttggt
     2221 tttgactaaa tcgtttttgg cgctgccaaa tggcacggca ccgctaaatg gtttgccgtc
     2281 gatagcgtca tgagtacgca atgcttccgg gcgcagccaa cgctgaacgc gggaaggcat
     2341 atccagacca atcagcagga tcaccacaaa tgtcaggctg aacgagagcg atgccagcgc
     2401 agtacccaga tccagacgtt gagcgatcaa cgcgccgatg attggggcca gtgcgccacc
     2461 caatgcgcca acgttgtagg taaagcccag gcccgctgca cgctggtcgg tatcgaaata
     2521 accgccaatc agtttcggta agatcccggc gatcccttgt ccaagcattt gctggaagaa
     2581 cagtaacaga ccgagcaccc agacgtttgc gccgccaatc gcaaataccg gaataatcag
     2641 cagctgcgag gccagcaggc tacaaacgta cgctttgcgg gttcccagcc agtcaccgag
     2701 gaagccacct acgcagcatc ccacggccgc gccaaagcca ctaaagaaca gcacattggc
     2761 gacagtatgc gggttataag ccagatcggt tttcagatac gttggcagca gcgcctgaat
     2821 cggccatgag tagaggaaag caaacaacac gaccaccatc agcattacgc ccgttggcca
     2881 gcgtttaccc gtactttgca ccataaagct gataaagatt gcggcgcata acagcccgag
     2941 aacagcgacg attgcagcat tttgcaggtt accggcgaag cagaaccaca gcgcagtagc
     3001 cgccgccagt gtcattacga tattggcaat gcgatgttca ccacggtaga gaatatccac
     3061 cattgtgcgt actggtgctt tacctgcgtg tttctctttc cagtcttccg cttccgggat
     3121 gtttttacgc agccagagag caaagatgat tggcaaaatg ccgataaaga acagcgcacg
     3181 ccaaccccag accggaacca ccaggctata gacctgagcg gcaacgacgg cccccacaga
     3241 gaagcctgaa atcaaaaaac cactggcttt gttacgcaga tgttttggcc agctttcaat
     3301 gacataggtg gcgctggaac cgtattcacc cgccatcccc atgccgatga ccagacgagc
     3361 gataaacatg gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg ccgagaagag
     3421 aacgatgctg gtgaccattg ccagacgacg cccgtagcgg tcacccatag cgccgagcat
     3481 caggccgccg aaccagcgag agataaaggc tgcggagatc agacttgccg cctgcaccgt
     3541 cgtcagcccg aattcaccct gtacttcggt gagtaccagg gcgattaaaa cgaaatcaaa
     3601 accgtcaagc agatatccca accaggcagc ggaaaatgcg cgccattgtg cacggttgag
     3661 atggcgatac cacgggatat tctgggttgt agtactcatt gtgagtctcc cgcggtgggc
     3721 gatgcccaca cgctttggta tgaaaattgt agggtacaga tgcgtttatt tcccctcacc
     3781 cggtaggggc gagcgagggg aaaacaactc acccgcgctc ttgcatcaac tgctgggcca
     3841 gcgccttcag ttctggcaga tatttttcat ctaccggtcc aaacggtttg cggcacagcg
     3901 gcacagaaac gacatccata taatgcagga cagttttcag gccgcggaat acgcccgttt
     3961 tgatcagtaa atcaatgact ttgttgcact cagtttgcag tttctgcgcg gtctggatat
     4021 cgccttcttt cagcgccttc acgatcccct gatatcgcca gcccatgatg ttgtaagtac
     4081 tgccgatacc accatcagcg cctgccagca agccggaagc gaagatttcg tcgtaaccgt
     4141 tatagagcac cagatcagga tgttcacgac ggatctgttc catctgatag agatcgccag
     4201 aagtctgttt cagcgcaccg acgccaggca acgtaaccag ggtgttgatc tgatccaggg
     4261 tcagttttac cccactcagg gctggaatgt tgtacaccac catcggcaaa ccatccgctg
     4321 aatcaataat tgcccgatag tgatcgcagt gttcttcaaa gctgaaagga tagtagaacg
     4381 gcgtgacggc ggagacggca tcgaagccat aacgtttagc cgatgccgca agttgttggc
     4441 tttcggcggt gctgacgcaa ccgacgtggg cgatgagttt aatcttacct ttcgcctctt
     4501 cggcgacgat ttccagtacc tgttcacgct cggaaaggct ttgtacaaag gcttcgccgg
     4561 tcgaaccacc cacgtataaa ccgtcgatgc cctgctgaat attgaactga accaggcggc
     4621 gcagactcgc tttatccagt gcttgttgtt gatcaaaagg agtcaggagt gcagccatta
     4681 cgccacgtaa attcgttgcc ataaatacct ctgaagtgat gcttgtctga taaacgatat
     4741 acctttatac ctgttatacc agatcaatta agcaacaccc catacagaaa gcttataatg
     4801 cgatctgctt cactaaagtg gcattatttc tttttgttgg tggtctgacc gaaagcgtgc
     4861 caggtagcag agacgctgtt gagatgcgat tgcaacgcac gatcggcttc gtcaggatca
     4921 tgacggcgga tcgcatcaac gatcgcaata tgctgttgat aactaacgtt gttatgttcg
     4981 tgcagtgcct gatcggtaac cgttgggcgt gcggcaataa gccagtcgag cagggcaacg
     5041 tggatcgcca tgaagattgg gttaccgggg atctccgcca gcacgcggtg gaaatcaacg
     5101 tctgaacgaa tgaatgccgc gttgttatcc agcgactgac tgttgatttc cagtgctttt
     5161 gccagcaaat cgatttgctc atcggtggcg tgttcagccg catagcgcac caggctggat
     5221 tcaaagaaca gacgtaattg ttcgaaatgg gcaatcccac caggatgaga aaggaaatct
     5281 ttcgccatgc cggaaagctc gccgatgata gtgtccgcag aaggtcgaga gacacgagcg
     5341 cgttcgccgt tgtttatttg caccagacct ttgcgtttta acgctgccag cgcttcacgc
     5401 acagaaggac gcccgacgtt aaagaacgcc atcagttcgc gttcagacgg taattgttca
     5461 ccttcgccaa attcacgacg gcggatcatc tgttccagct cttcttccac catttcggag
     5521 agttttttac gcgccagcgg gcggctacgc aagttgcgac caattgcagg tgaagaatct
     5581 tcggtttgcg aatcaaatgc gttcataagg cccattctgt aaggtcagtg tgattaacat
     5641 catcagtgac atcctatcac aggattgaaa gtaggggaaa atggcaggtt tttctctttg
     5701 tgcctcatca ttaccataat taacggaata attaactatt gcgaaaaatt aatgtaacgc
     5761 agataaacac at
//
LOCUS       DAJVQR010000171         5683 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVQR010000171 DAJVQR010000000
VERSION     DAJVQR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..133)
                     /locus_tag="OQM52_003891"
     CDS             complement(<1..133)
                     /locus_tag="OQM52_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCS1565544.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     "
     gene            369..641
                     /locus_tag="OQM52_003892"
     CDS             369..641
                     /locus_tag="OQM52_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1565545.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(607..1014)
                     /gene="nudG"
                     /locus_tag="OQM52_003893"
     CDS             complement(607..1014)
                     /gene="nudG"
                     /locus_tag="OQM52_003893"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1565546.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1101..1721
                     /locus_tag="OQM52_003894"
     CDS             1101..1721
                     /locus_tag="OQM52_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1565547.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1730..3037)
                     /gene="ynjE"
                     /locus_tag="OQM52_003895"
                     /pseudo
     CDS             complement(1730..3037)
                     /gene="ynjE"
                     /locus_tag="OQM52_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3104..3757)
                     /locus_tag="OQM52_003896"
     CDS             complement(3104..3757)
                     /locus_tag="OQM52_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1565548.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3757..5229)
                     /locus_tag="OQM52_003897"
                     /pseudo
     CDS             complement(3757..5229)
                     /locus_tag="OQM52_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5202..>5683)
                     /locus_tag="OQM52_003898"
     CDS             complement(5202..>5683)
                     /locus_tag="OQM52_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1565549.1"
                     /translation="YLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQ
                     QKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKAD
                     PVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
BASE COUNT         1353 a         1520 c         1482 g         1328 t
ORIGIN      
        1 attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga acggcttgcg
       61 cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac tccagagaat
      121 atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt atgttgttta
      181 tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta agcaagaaaa
      241 aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat gcgataactc
      301 agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat aacgttaagg
      361 agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta attgcactgg
      421 ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg ttcagttcag
      481 gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa aattattctg
      541 aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat gacaaaaccc
      601 ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta ataatggaat
      661 gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga ccagcgcctg
      721 atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat gaagatggat
      781 aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag ttgcttcgat
      841 gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat ccagctcgac
      901 tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc gttgcgcgag
      961 taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt tcataaatta
     1021 cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa tcagaaaggc
     1081 tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt aaaccgttgt
     1141 tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt ttaacattag
     1201 tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg tatctggcgg
     1261 cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg ctggcgcggc
     1321 gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt ctcttttacg
     1381 cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg gcaggcggct
     1441 ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct gcactggcag
     1501 caaaacatca gattgataac cccggttatg cacataagtc gttttactat ctggggggat
     1561 taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc cctgcatggt
     1621 ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt acgcgggtgt
     1681 ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt tatttgctac
     1741 tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc cattcgtacc
     1801 agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac ataaaggttt
     1861 cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt ttgatattcc
     1921 atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc gggttataga
     1981 aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg gctatttcac
     2041 cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc cacgaacgaa
     2101 tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc atatcgagca
     2161 tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc actttcggtg
     2221 gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg ccatccagca
     2281 gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct ccagcgtata
     2341 cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt gccagcatcg
     2401 cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact tcgttggtat
     2461 cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg ccctaggccg
     2521 cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct tgttgcaggt
     2581 catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt agacgggaag
     2641 gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt tgcagtcgcg
     2701 ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca tcggctttca
     2761 ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc agccagctag
     2821 cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt tgtggccagc
     2881 cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc tgaagttggt
     2941 caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc agccctaaag
     3001 ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac attgtagttt
     3061 tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc agtttgttgt
     3121 aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca tcctggagat
     3181 cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac acccactggc
     3241 gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg agtaacgctt
     3301 ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg ccagacaaag
     3361 tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca tcattcacgg
     3421 cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa ttttgcccga
     3481 cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga cgctgtgcgg
     3541 tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa gaaaactgtc
     3601 cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca gacggcccca
     3661 ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc aagcggcttt
     3721 ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt gtctgacata
     3781 acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta acaacagttg
     3841 ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg ccaccgcctc
     3901 agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat actgcgcaat
     3961 actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga gtgggcattt
     4021 cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca aaattaaccg
     4081 tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca ttacccacag
     4141 cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata gcgccagcgt
     4201 gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata gccacaactg
     4261 gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga tgaatgttgc
     4321 taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg actgatccgc
     4381 gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac tcgccagcgt
     4441 agtgcctggt aataaaggtg tggcaggcat tgcgaatgcc atctacgcgg ggaatagtgc
     4501 gccgccagct gcgccaaaac gtaggcggcg agtaacagca tcagcagcag actggcgagt
     4561 gcgcctttcg tttgttgatc cgcgtcgccc tgagttaacc actgccagct aattactgcc
     4621 agcgtcggtg gattacctgg cccaagaata atcgccacat ccacgaccga aagcgaccag
     4681 gcaacaatcg ccagcatcgc cattgccagc ggctgtagcc cagcgaatcc agcacaatga
     4741 cctgctgcaa cagccgtttt tcgctcaata ctgccactaa gatccacagc agaaatgcgc
     4801 tttctttcac tgcaagggtg aggcccagac cgatccaaat cggtccattg gcggagtgaa
     4861 atacgggaaa tagtcatata gcagccctcc gtcagcaaag agcagcaggg cgctggtggc
     4921 aaaagccaca tggggaatgg cgagcagcca cggcagacgg gcgcacatac gctgccattt
     4981 cggcccaggc catagcgcca caatcaccag cagggcaatc aacaatgccc cgaccgccgc
     5041 gatggttgtc gacaccagcg ttgccagtaa tgcctgcggt aactgtggat cggcaaataa
     5101 cgcctgccag cgtgtcaacg acaacgcagg cgatatcagc gtgagagctg ccgggatcag
     5161 cggtgcataa atcaccgcca ccatcgccca cagcaaaaag attaatgcgt accgtaacgg
     5221 cgtaaccatt cttgttccag tgcattcacc catcctgcgt gcggttcagc cagtaccggc
     5281 ggcagatcct gcggcattct tgattgcaat atttcgcgct gcccgtcagg cagtttttgc
     5341 ggatcgagaa cagaaggatc gccccagaca acgggatctg ctttacgcag ttgcgcatcg
     5401 ggtgagagca ggaaattggc aactaccttc gccgcagcac tggcattcgc gttggctgga
     5461 atggtgacga aatgcacgtt gccaatcatc ccctcgcgaa agccaaaact gtaactgctt
     5521 gccggcaaat cgccgctggc gattttttgc tgcgcatgtg cggggttaaa ggtcagcgac
     5581 aggcgcaatg tgccggcttt cagcagagca tccatccgcg cgggcgaagg cgggaaatcc
     5641 tttccttcgc gccacaaata cggatgcagt gcatcaagat att
//
LOCUS       DAJVQR010000172         5618 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVQR010000172 DAJVQR010000000
VERSION     DAJVQR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..170)
                     /locus_tag="OQM52_003899"
     CDS             complement(<1..170)
                     /locus_tag="OQM52_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565550.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OQM52_003900"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OQM52_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1565551.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OQM52_003901"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OQM52_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OQM52_003902"
     CDS             complement(3616..4383)
                     /locus_tag="OQM52_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1565552.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="OQM52_003903"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="OQM52_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5618
                     /locus_tag="OQM52_003904"
     CDS             5484..>5618
                     /locus_tag="OQM52_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565553.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     A"
BASE COUNT         1393 a         1445 c         1455 g         1325 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgca
//
LOCUS       DAJVQR010000173         5601 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVQR010000173 DAJVQR010000000
VERSION     DAJVQR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..411
                     /locus_tag="OQM52_003905"
     CDS             <1..411
                     /locus_tag="OQM52_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565554.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OQM52_003906"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OQM52_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OQM52_003907"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OQM52_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCS1565555.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DAJVQR010000174         5587 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVQR010000174 DAJVQR010000000
VERSION     DAJVQR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(1..279)
                     /locus_tag="OQM52_003908"
     CDS             complement(1..279)
                     /locus_tag="OQM52_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565556.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OQM52_003909"
     CDS             220..405
                     /locus_tag="OQM52_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565557.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OQM52_003910"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OQM52_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1565558.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OQM52_003911"
     CDS             complement(1254..1670)
                     /locus_tag="OQM52_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565559.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OQM52_003912"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OQM52_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OQM52_003913"
     CDS             1837..2862
                     /locus_tag="OQM52_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1565560.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OQM52_003914"
     tRNA            complement(2915..3004)
                     /locus_tag="OQM52_003914"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OQM52_003915"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OQM52_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1565561.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OQM52_003916"
     tRNA            3996..4071
                     /locus_tag="OQM52_003916"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="OQM52_003917"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="OQM52_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1324 c         1292 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcacctc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaaca
//
LOCUS       DAJVQR010000175         5553 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVQR010000175 DAJVQR010000000
VERSION     DAJVQR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..242
                     /locus_tag="OQM52_003918"
     CDS             <1..242
                     /locus_tag="OQM52_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565562.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(239..1180)
                     /locus_tag="OQM52_003919"
     CDS             complement(239..1180)
                     /locus_tag="OQM52_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1565563.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1192..1681)
                     /locus_tag="OQM52_003920"
                     /pseudo
     CDS             complement(1192..1681)
                     /locus_tag="OQM52_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1685..2905)
                     /locus_tag="OQM52_003921"
     CDS             complement(1685..2905)
                     /locus_tag="OQM52_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1565564.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2909..3652)
                     /locus_tag="OQM52_003922"
     CDS             complement(2909..3652)
                     /locus_tag="OQM52_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS1565565.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3543..5009)
                     /locus_tag="OQM52_003923"
     CDS             complement(3543..5009)
                     /locus_tag="OQM52_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCS1565566.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5009..5242)
                     /locus_tag="OQM52_003924"
     CDS             complement(5009..5242)
                     /locus_tag="OQM52_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCS1565567.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5230..>5553)
                     /locus_tag="OQM52_003925"
     CDS             complement(5230..>5553)
                     /locus_tag="OQM52_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terL-like domain protein"
                     /protein_id="HCS1565568.1"
                     /translation="LKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSEN
                     AYARRVNYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENS
                     ILWMS"
BASE COUNT         1182 a         1567 c         1399 g         1405 t
ORIGIN      
        1 tctttggcat tttttacctc gttacgcgcg tctgtggctt tgccaacgga tacttccgcg
       61 tgcgcacgag tgaaccagtg cgtggcaacg tcttcctcca caacatgacg gcctttaaca
      121 aactcgcgcc gagaaccgtc gggaagcgtg agcacaaacg gggtatgtac gtgtattact
      181 gcatcatttt ttgccatcgg gtcatcctta atggccccgc cagggggcca tgtggctgtt
      241 aaatgccatc aacgtacgaa atggtttctt tgtacactgg ctcgactgca cccagcttgc
      301 cgtagtaagt gacgatctga tacagaccgc gatactgcac cggcacgctc tgaagcggaa
      361 ccagcgggta gcggacgtat tttttatcgt tggtgtacgc aactatgcga tccttattcc
      421 ccacaccacg gcctttcagc catttaaccg cgcggatatt cagcggaaca ccgttctggt
      481 gatagctgat ggtgttggtc tgaaggttcg tcaacaggga ctggttacct gcagatgaaa
      541 cgatgatgct ggacaacaga gcaaactgct caggcgggat cagcaaatca cgcggaacca
      601 cagagtaacc agaagcggcc cacgcatcag acagcacctg gttaatgctt gcgcggattt
      661 cgtccggtgt tgaggttgcc cacgttttgg cagcgttgtt gacaggcacg ccgtccaggg
      721 taacaaggcc tttcaggttt aatgcggaat cgccaacata tacctgttca tcgttatcca
      781 tctgccattt cagttgcata ccgtcatact tctgcgtatc aatcgggcgg ccgacctgct
      841 gagcagcctg caattctatg accgtccagc caagttccat cccccacagg ttcagcgggt
      901 taccggattt gccgatatcc acgttcacgc cagcaatagc ggttgagtct ttgcctaccc
      961 agttttttcc attcggattt gcgccagtac ccgcagcggc gaagctggta ttcgtccagc
     1021 tggaaatgtc atctgcgatg gaggcgtctt cacgcagttg aatatcgcgg ctccaggtgt
     1081 accccaccag tggcaggttc agcgtctggt cgagtctctc cagctccccg atgagaaagg
     1141 caccagagct gtcaacggtt gcctgatcaa aagtaatcat tcgtctgttc cttaaatctt
     1201 ccaggaaatt tctgcattgc cgtcagcatc accggcacct gtgaattcag cgttggtcag
     1261 caccacgttt ttgccactga ctgacgtgga catgaatcca cccagaggca ctttgatgga
     1321 ttcatcagtg gagatcgcct tttttgatag tgctggcatc aaaatcagaa ccgagattaa
     1381 cggtcacgta gccacgcttc atggcgtcgc ccgggaagtt cttgcctgtt cccacctggc
     1441 gaaccatgtc cggctgcgac gtggtcggat aagggcgcac gtagatcccc ttcaccttgt
     1501 ctgcggtatc accatctgcc agcggcacga aaaaaccgtc atcatcgtat ttaccagcca
     1561 ggccataggc agcgaaggcg ttatcggatt taaggaccac cggttcgacg gttaagtcct
     1621 gcgggcgaga gacagccccg gcaatgccaa caggcatccg gtacagaaat acattattca
     1681 ttttttaccc tttacggttt gcccagaatt cagcgttttg tttgttcagg gaagcgatac
     1741 tggtcatgcc catgtttagg cgctgtgcat cgccggtggt ggcgcgggtg tttcgccctt
     1801 tggcaacctc agacacggca ttaaacgcca tgtcgaccga ttgtttcggt aatttgcgga
     1861 tatccgcatc accgactatc tggcgaacca gcgttttgtc agcagaagcc agaacctcgc
     1921 gtttgaacgc ggtcggtttc atcttacggc tcagatcgat acccggaacg ataacttcgg
     1981 cacgccaggc tgagtcacca gtaatcgtgg tttcctcttc atcgtcctcg ccgtcaccgg
     2041 tcggattatc gtcaggctta ttatcgttat cgcccgtggc atttctttcc agcttagcca
     2101 gcagggcttt cagtaatgtt ttgaggtcat catcactgtc gccggttgga tctccgccca
     2161 tctctggtgc tttgtccggt agtggttgct gcggggacag gttgatgttg agattaacgc
     2221 cctgcggcaa atccccctca tctcctgtaa ccgatgcggg agccgactcc accagttcgt
     2281 tcatggtgtc ggcatctcct gtcttgatgg ccgcacgcat gcggttccac cagtttttct
     2341 tttgatttgc cattgtgtct ctgtctccaa ttgcacaacg atttccggct ctgcctttag
     2401 ggacaagagc cacatggttt ccggtaatat cgacctgctc ggctttacct ggctcggtct
     2461 gctcgtactc cgcgtcatag ccgcacgaca cttcgcgcag gccatcttcg ataagctgaa
     2521 tggcgctttc gtctttgacg ataaggtcag ccagcatcaa atcagactgc tcacccgtcc
     2581 cgcgccggac attctggagg tgcccgacag caagcacttt ccagttctcg ggatttacca
     2641 gccgcacatt cccgttttca tcttcaggat gcaggatcgt gatgctcatc ccttcgaatg
     2701 aggcaagcgt ggccggatgg aatacctgct caggagaacg cgtgacgact atttcaacga
     2761 acttatcggg tttcagtttt ggcaggtcat cagcaccata gagctgctta cctgttcgtc
     2821 ctatcggcac gtctttgcac agcaacgagc cgtcagccag gcggtatcgg gtttccccga
     2881 gcctggtctc aaaaaagtat ttcatgggtt atgctgccct tttcattgcg aatggaacgc
     2941 gaacaaacac gatctcttta tagcagcggc agtttggcag ctcgcccgcg tggccggtca
     3001 tgccgtccaa agttggaggt gaatcccagc ggacgaattt acctttcatg tgatcgtggg
     3061 aatctcggac atcgccatca tcggctgtac gccaaatata accatccgag ccaatagcta
     3121 tcgctcgggc catatccagc gcgcccgttg ctcttcccag ttccgttcgg gcaatcagat
     3181 cggccctaga cttagcaaca tcgccggtag atgcaatctc tttagcaaac tgctccgccc
     3241 gaccaccggt gacaacagcc tctatcgctt tgttctggat gtcgtacacc ctgtctgcgg
     3301 cctcgagagg cagagatttg atgtacttga cctgttcggc gatgatggat tgcatcacct
     3361 ggcccacagg agcgctttcc acaagattgc ggagctcgcg actgatgttc ttgctgtgtt
     3421 gccgccaaac tttctcgttc tgccgggtta ggtccgcagt aaagttttcc gcgacctttg
     3481 tcgcccaggg ggtgatgatt tcactgtaac gctccagcgc cgcaataatt tccgtgatac
     3541 tgtcatttga accatcgtag cgaccattta cgatgtctcc gaccgccctc gctatcctgc
     3601 gtaggctggt tcgataccgg atttccgcct gacggctcct gcggttcgtc atcaggttcg
     3661 ccgatgccgg gcggcgcttc atcttcggca ttctcgatgt cctcgttggt aatggatgcc
     3721 cctatgccgg ttacgtcaga attttctcgc aaatcagtca tagcggcttt cagtgtcatc
     3781 agaccatcac ccagcgccgt actgattgcg ttggtggtgt ttaacgccac cgttgagcga
     3841 tcgacatcag acatttgcca gagcgggtta aactcaaacg tgaaatcgtc cggcagcggc
     3901 ttgccaagtt ccgaacgatg catgatgtcc agtatccgcc gcaccggaag acgtaaacgc
     3961 ctctcctgca acgaactgat gcgatcgtaa tagttggcaa ggtctgcatc accggtagaa
     4021 aatcctttcg gggactgtcc gaacaaccgc accagtggga taccaacagc gccactaatc
     4081 tgttctgcaa actgcgaaag gatgtcatcc agaccactga agctgtactg atgggtttca
     4141 aacttatccc gcgagtccat gagcgtcatg ccttcattac tctggaactg tcgaatcagg
     4201 tcgatattct tcagcaacgc ttcatacgca ggaccaccaa gtgcgataag ctcgcgtagc
     4261 ttctccacgc tgtaggtacg caaatgcgcc ttgtagacca gctgcgccgc gccgacagta
     4321 gcgctgtcga acgcggtaag acgatcccag atacgctcta caaccgacat tccccattcg
     4381 ttctcggtca tcttctgctg aaatggcaac gtgacgccat caaagcgaat caggcgactg
     4441 tgatgaatgc gccaggcagg aattcccgtt gctgtggtca ccacatcgta aaactcaggt
     4501 ttacccaggt ccggccccat atctttaatg cggcgggtca gtgccgggtc gaccatccag
     4561 cggtcgagcg ggagaatccc cttaaacttg cccttaccga tggtttcggg tcgcagcggg
     4621 gtcattggtg cctgcccctc aatcatgatg aaacccaccg cgccgccgta gaggcgcgac
     4681 catttcagca cgtcattcag cgcatcccag atttgcaact catccagttg tgattcgaga
     4741 atgccacgat cttttgcatc aatttccgaa gtgatgcgaa tgcctttgcg ggtcatatca
     4801 tccgggatag catcgactgc ttcgccgatg atccaggatg aacgatagga ccattccacc
     4861 agcatgcggt tacgactggt gaaattagcc cggtaggtgg atgctgagtg ctggttaggt
     4921 gtctgcatcc ctactcgggc aataaaattc tcataaccat cagctgtggc ctgcacagtt
     4981 cgccgcaggg cttgtttgtt tcgtgccatc aggcctgtct ccccagctgt tcccagatat
     5041 ccagcgatgt atcgattggc gcgaaggcca taatgaatgc atcagcaacg tttggtgacg
     5101 gtatctcgcg ttttgcgagg tcttttttac tttcgaccat cacacgtccg ttacggtcga
     5161 aatcacgatg aggtgttgtc agttccagtt tcagcttttc aagcaacgga caacgagaat
     5221 ctatgctgat cagctcatcc acaggatact gttctccgtt gttaatggcg ttaaacgtat
     5281 ttctgaaacg gtcagccacc atgcctgagc cttaagattt gcgaaaaagt ctttgttggg
     5341 gatgccgttg tactcgtcat ctggctcatg cacaccagca ccggcgttaa acctctggta
     5401 attcacacgt cgcgcgtatg cgttctcgct cttccggtca gcgttaattt cagagaattt
     5461 agcaccggca gacgcaccaa caccgataga gtcgtaaaca atatctgctt cacgctccag
     5521 cgccgcctga taagtacgct ggcagctctt cag
//
LOCUS       DAJVQR010000176         5426 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVQR010000176 DAJVQR010000000
VERSION     DAJVQR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            137..1063
                     /gene="tauA"
                     /locus_tag="OQM52_003926"
     CDS             137..1063
                     /gene="tauA"
                     /locus_tag="OQM52_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1565569.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1076..1843
                     /gene="tauB"
                     /locus_tag="OQM52_003927"
     CDS             1076..1843
                     /gene="tauB"
                     /locus_tag="OQM52_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1565570.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1840..2667
                     /gene="tauC"
                     /locus_tag="OQM52_003928"
     CDS             1840..2667
                     /gene="tauC"
                     /locus_tag="OQM52_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1565571.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2664..3515
                     /gene="tauD"
                     /locus_tag="OQM52_003929"
     CDS             2664..3515
                     /gene="tauD"
                     /locus_tag="OQM52_003929"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1565572.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3621..4595)
                     /gene="hemB"
                     /locus_tag="OQM52_003930"
     CDS             complement(3621..4595)
                     /gene="hemB"
                     /locus_tag="OQM52_003930"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1565573.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5119..>5426
                     /locus_tag="OQM52_003931"
     CDS             5119..>5426
                     /locus_tag="OQM52_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1565574.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTSAN
                     "
BASE COUNT         1258 a         1363 c         1490 g         1315 t
ORIGIN      
        1 tgagcatttt taactcgttt tatcttgaat gcaatcggcg cataacttat tgaatcccat
       61 caaatcccgt ctttttttac ccattcccgg ttatcttgaa taaattacct agtcatataa
      121 cacacttctt gccgcactgg cattcatcgc ttttcaggca caggcggtga acgtcaccgt
      181 ggcgtatcaa acctcagccg aaccggcgaa agtggctcag gccgacaaca cctttgctaa
      241 agagagcggg gcgagcgtgg actggcgtaa gtttgacagc ggagccagca tcgtgcgggc
      301 gctggcttct ggcgacgtgc aaatcggcaa cctcggttcc agcccattag cggttgcagc
      361 cagccaacag gtgccgattg aagtcttctt gctggcgtca aaactgggta actccgaagc
      421 gctggtggta aagaaaacta tcagcaaacc cgaagatttg attggtaaac gcattgccgt
      481 accgtttatc tccaccaccc actacagcct gctggcggcg ctgaaacact gggggattaa
      541 accagggcta gtggagattg tgaacctgca gccgcccgcg attatcgccg catggcagcg
      601 gggtgatatt gatggtgctt atgtctgggc accggcggtt aacgccctgg aaaaagacgg
      661 caaggtgctg accgattctg aacaggtcgg gcagtggggt gcgccaacgc tggacgtctg
      721 ggtggtgcgc aaagattttg ccgagaaaca tcctgaggtc gtgaaagcgt tcgccaaaag
      781 cgccatcgat gcacagcaac cgtacattgc taaccctgac gcgtggctga aacagccgga
      841 aaacatcagc aaactggcgc gtttaagcgg cgtgcctgaa ggtgacgttc cggggctggt
      901 gaagggaaat acctatctga cgccgcagca acaaacggca gaactgaccg gaccggtgaa
      961 caaagcaatc atcgacaccg cgcagttttt gaaagagcag ggcaaggtcc cggctgtagc
     1021 gaatgattac agccagtacg ttacctcgcg cttcgtgcaa taaaaggagg cgcagatgct
     1081 gcaaatctct catctctatg ccgattatgg cggcaaaccg gcactggaag atatcaacct
     1141 gacgctggaa agcggcgagc tactggtggt gctggggccg tccggctgcg gtaaaaccac
     1201 cctgctgaat ctgattgccg gttttgtgcc ttatcagcat ggcagcattc aactggcggg
     1261 taagcgtatt gagggaccgg gagcagagcg tggcgtagtt tttcagaatg aagggctgct
     1321 accgtggcgc aatgtacagg acaacgtggc gttcggcctg caattggcag gtatagagaa
     1381 aatgcagcga ctggaaatcg cgcaccagat gctgaaaaaa gtggggctgg aaggcgcaga
     1441 aaaacgctat atctggcagc tttccgatgg tcaacgtcag cgggtgggga ttgcccgcgc
     1501 gctggcggcg aatccccagc tgttattact cgacgaaccg tttggtgcgc tggacgcctt
     1561 cacccgcgac cagatgcaaa ctctgctgct gaaactctgg caggagacgg gcaagcaggt
     1621 gctgttgatt acccacgata tagaagaagc ggtgtttatg gcgactgaac tggttctgct
     1681 ttcatccggc cctggccgtg tgctggagcg gctgcggctc aactttgctc gccgctttgt
     1741 tgcgggagag tcgagccgca gcatcaagtc cgatccacaa ttcatcgcca tgcgcgaata
     1801 tgttttaagc cgcgtatttg agcaacggga ggcgttctca tgagtgtgct cattaatgaa
     1861 aaactgcatt cgcggcggct gaaatggcgc tggccgctct cgcgtcaggt gaccttaagc
     1921 attggcacgt tagcggtttt actcactgta tggtggacgg tggcggcgct gcaactgatt
     1981 agcccgctgt ttttgccgcc gccgcaacaa gtactggcaa aactgctcac cattgccgga
     2041 ccgcagggat ttatggacgc cacgctgtgg cagcatctgg cagccagcct gacgcgcatt
     2101 gtgctggcgc tactcgcggc ggtggtgatc ggtattccgg tcgggatcgc catggggcta
     2161 agcccgacgg tgcgcggcat tctggatccg gtaatcgaac tttatcgtcc ggtgccaccg
     2221 ctggcttatt tgccgctgat ggtgatctgg tttggtattg gtgaaacctc gaagatctta
     2281 ctgatctatt tagcgatttt tgcgccggtg gcgatgtcgg cgctggcagg ggtgaaaagc
     2341 gcgcagcagg ttcgcattcg tgccgcccag tcgctgggtg ccagccgtgc gcaggtgctg
     2401 tggtttgtca ttttgcccgg cgcgctgccg gaaatcctca ccggattacg cattggtctg
     2461 ggtgtgggct ggtctacgct ggtagcggcg gagctgattg ccgcgacacg cggtttagga
     2521 tttatggttc agtcagcggg tgaatttctc gcgactgacg tggtactggc ggggatcgcg
     2581 gtgattgcga ttatcgcctt tcttttagaa ctgggtctgc gcgcgttaca gcgccgtctg
     2641 acgccctggc atggagaagt acaatgagtg aacgtctgag cattaccccg ctggggccgt
     2701 atatcggcgc acaaatttcg ggtgccgacc tgacgcgccc gttaagcgat aatcagtttg
     2761 aacagcttta ccatgcggtg ctgcgccatc aggtggtatt tctgcgcgat caagctatta
     2821 cgccgcagca gcaacgcgcg ctggcccagc gttttggcga actgcatatt caccctgttt
     2881 accctcatgc cgaaggggtt gacgagatca tcgtgctgga tacccataac gataatccgc
     2941 cggataacga caactggcat accgatgtga catttattga aacgccaccc gcaggggcga
     3001 ttctggcagc taaagagtta ccttcgaccg gcggtgatac gctctggacc agcggtattg
     3061 cggcctatga ggcgctctct gttcccttcc gccagctgct gagctggctg cgtgcggagc
     3121 atgatttccg taaatcattc ccggaataca aataccgcaa aaccgaggag gaacatcaac
     3181 gctggcgcga ggcggtcgcg aaaaatcctc cgttgctaca tccggtggtg cgaacgcatc
     3241 cggtgagcgg taaacaggcg ctgtttgtga atgaaggctt tactacgcga attgtcgatg
     3301 tcagcgagaa agagagcgaa gccttgttag gctttttatt cgcccatatc actaaaccgg
     3361 agtttcaggt gcgttggcgc tggcaaccga atgatattgc gatttgggat aaccgcgtga
     3421 ctcagcacta tgccaatgcc gattacctgc cacagcggcg gattatgcat cgggcgacga
     3481 tccttgggga taaaccgttt tatcgggcgg ggtgatacga gtgcggacgg ttccctcgcc
     3541 cccttgggga gagggttagg gtgagggggc gttcactgta ctttcaacag gttaactccc
     3601 cctttctgag aggaaaaaaa ttaacgcaga atcttcttct cagccaaatc cagcgcaaag
     3661 tagctgaaaa tcagatccgc acccgcacgc ttaattgaac ctaagctttc gagcacgact
     3721 ttctcttcat ctatagcacc cgccagcgcg gcgaacttaa tcatcgcgta ctcaccgctc
     3781 acctgatacg cgccaatcgg caattcagta cgttcacgca gctcacgcac gatgtcgagg
     3841 tacgctccag caggtttaac catcaggcag tctgcgccct gggcttcatc cagcagtgac
     3901 tcacgaatcg cctcgcgacg gttcattggg ttcatctgat agcttttgcg gtcgcctttt
     3961 aatgcgcttc ctgcagcttc acggaacggg ccataaaagg aagaggcgaa cttggtcgaa
     4021 tacgacataa tcgccgtatc tttaaagcct gcagcgtcca gcgcctgacg aatcgcctgt
     4081 acctggccgt ccatcgcggc ggaaggggcg atgaagtctg cgcctgcagc agctgcaacc
     4141 acggcttgct tgcctaaatt ttccagagtc gcgtcgttgt cgacgccatg ctcgcacagc
     4201 acaccgcagt gaccgtgaga agtgtattca cagaaacaag tgtctgacat gacgatcatt
     4261 tctggcacag tctgcttgca gatgcgcgac atacgcgcca ccagtccatc ttcccgccag
     4321 gcgtcgctgc cggtttcatc ggtatggtga gagatgccaa aagtcattac ggaacggata
     4381 ccggcgttgg cgatgcgttc aatttcgcgt gccagatgtt tctctggaat gcgcatcacg
     4441 cctggcatgg cttcaacggc tttgtagtcg tcaatttctt cttcaacaaa gatcggcaac
     4501 accaggtcgt taaggctaag tgttgtctct tcaaacatag cgcgcagcgc aggagatttg
     4561 cgcaggcgac gagggcgttg gattaagtct gtcatggtct gcctgatgtt tgtggaatta
     4621 aggtgcacag tatacctgaa gcggggcagg agtgttttac gaaagttgtc gcgatgttga
     4681 caagaagaga atgaaagaga gaccaggaca tctttgtaat agaaatagca ataatttcat
     4741 attcactgaa aatattttta atctttattt atagctcttg gttattattt tttggcgttt
     4801 ggttgtgctg ctataaattg aataattcaa actttgttgt atattgataa gatatttatc
     4861 tggtttgtat ttttgatatt tataacgcgt atgcatcaaa aataacatta tctgcaacat
     4921 actgaaatat cgtcctttta tcaatttgat gcgtgttttt ctccataaat tatatatgtc
     4981 cacattcgga cttaggggaa agaataattg aaccattcgt ctgtaacgca gcataatcgt
     5041 tagcgcgaaa cataatatgt tttctatgca ttgataattg atggatcaac ttattacgtc
     5101 cctgaggagg gatgacaaat gcactcctgg aaaaagaaac ttgtagtatc acaattagca
     5161 ttggcttgca ctctggctat cacctctcag gctaatgcag cgaccaacga tatttctggt
     5221 caaacttaca atactttcca tcactacaac gacgccacct atgctgatga cgtttactat
     5281 gatggttatg taggctggaa caactatgcc gctgatagct attacaacgg cgatatctac
     5341 ccggtcatta ataacgctac cgttaacggc gtgatttcta cctactatct ggacgacggt
     5401 atttctacca ataccagcgc caatag
//
LOCUS       DAJVQR010000177         5388 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVQR010000177 DAJVQR010000000
VERSION     DAJVQR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..497
                     /locus_tag="OQM52_003932"
     CDS             <1..497
                     /locus_tag="OQM52_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCS1565575.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OQM52_003933"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OQM52_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OQM52_003934"
     CDS             2500..3114
                     /locus_tag="OQM52_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1565576.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OQM52_003935"
     CDS             3114..3443
                     /locus_tag="OQM52_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1565577.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OQM52_003936"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OQM52_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1565578.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OQM52_003937"
     CDS             4529..4840
                     /locus_tag="OQM52_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1565579.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4975..5373
                     /locus_tag="OQM52_003938"
     CDS             4975..5373
                     /locus_tag="OQM52_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1565580.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1175 a         1340 c         1421 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacc
//
LOCUS       DAJVQR010000178         5326 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVQR010000178 DAJVQR010000000
VERSION     DAJVQR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(25..762)
                     /gene="bamD"
                     /locus_tag="OQM52_003939"
     CDS             complement(25..762)
                     /gene="bamD"
                     /locus_tag="OQM52_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1565581.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            897..1877
                     /gene="rluD"
                     /locus_tag="OQM52_003940"
     CDS             897..1877
                     /gene="rluD"
                     /locus_tag="OQM52_003940"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1565582.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1874..2605
                     /gene="pgeF"
                     /locus_tag="OQM52_003941"
     CDS             1874..2605
                     /gene="pgeF"
                     /locus_tag="OQM52_003941"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1565583.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2735..5308
                     /gene="clpB"
                     /locus_tag="OQM52_003942"
     CDS             2735..5308
                     /gene="clpB"
                     /locus_tag="OQM52_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1565584.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1308 a         1287 c         1451 g         1280 t
ORIGIN      
        1 gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga tgattttcgc
       61 tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca tcagcggcag
      121 cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta tgcggttaac
      181 gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt catatttcgc
      241 cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact gactgttcgg
      301 atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt gaggatcgcg
      361 atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg ccatattggt
      421 caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa ggcgaataaa
      481 acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg cgtagatgag
      541 atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta acgcttccag
      601 ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag tcgcgtaaat
      661 ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg ccaaaaacag
      721 gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt gactttcctc
      781 aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct acaatagcac
      841 actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca gtatatatgg
      901 cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa cgcttagatc
      961 aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa tggatcctcg
     1021 accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa gtattgggtg
     1081 gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa ccgcaggata
     1141 tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa ccgcgcgacc
     1201 tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg ttgcttcatt
     1261 actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg gataaagaca
     1321 ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta gtcgaatctt
     1381 tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat atgaccgctg
     1441 gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac atggcggtgc
     1501 atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc cgtgtgcaca
     1561 cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg catatggccc
     1621 atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt ccgccaaaag
     1681 gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg cttcatgcaa
     1741 ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat gcgcctattc
     1801 cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag cataaggatg
     1861 aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa aaggtgttgc
     1921 ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact cactcaacct
     1981 cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc gactttttgc
     2041 tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca aagatgtgct
     2101 taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata gcaatacgcc
     2161 tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt gcaatcgagc
     2221 gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag gcgtgctgga
     2281 agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt tagggccggc
     2341 aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta tggcagtaga
     2401 cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg atatttatca
     2461 gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg gcgaccgttg
     2521 tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca ccggtcgtat
     2581 ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta cgcgtgattt
     2641 tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct catttaatct
     2701 ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc ttactaataa
     2761 attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca accaattcat
     2821 cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg ttagtccttt
     2881 attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc aggcattaaa
     2941 tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg atctggtgcg
     3001 cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta tctcgtcaga
     3061 actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc tgaaagcaac
     3121 aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag gtgaaagcgt
     3181 gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca tcgaccttac
     3241 cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag aaattcgccg
     3301 taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg gtgaacccgg
     3361 cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg gcgaagtgcc
     3421 ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg tggctggggc
     3481 gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc ttgccaaaca
     3541 ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg cgggtaaagc
     3601 cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg gtgaattgca
     3661 ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag atgctgcgct
     3721 ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata ccattgcgat
     3781 tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta ctgacccggc
     3841 aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc tgccggataa
     3901 agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg actcaaaacc
     3961 agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac aacaggcgtt
     4021 aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg aagaactgag
     4081 cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga aggcatcgct
     4141 ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta ttgaacaggc
     4201 tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa tcccggaact
     4261 ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc tgttgcgtaa
     4321 taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga ttccggtttc
     4381 tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac tgcaccatcg
     4441 cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc gtagccgtgc
     4501 ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc caacgggtgt
     4561 ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg acgaggcgat
     4621 ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc tggttggtgc
     4681 gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg tgcgtcgtcg
     4741 tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg tcttcaatat
     4801 tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa cggtcgactt
     4861 ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc aggaacgctt
     4921 cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta gccataactt
     4981 ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc tgggtgaaca
     5041 gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc tggaagaacg
     5101 tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga acggttacga
     5161 tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg aaaacccgct
     5221 ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc tggaagttaa
     5281 tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttc
//
LOCUS       DAJVQR010000179         5143 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVQR010000179 DAJVQR010000000
VERSION     DAJVQR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..233
                     /locus_tag="OQM52_003943"
     CDS             <1..233
                     /locus_tag="OQM52_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565585.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            492..676
                     /locus_tag="OQM52_003944"
                     /pseudo
     CDS             492..676
                     /locus_tag="OQM52_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            819..1040
                     /locus_tag="OQM52_003945"
                     /pseudo
     CDS             819..1040
                     /locus_tag="OQM52_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1061..1123)
                     /locus_tag="OQM52_003946"
                     /pseudo
     CDS             complement(1061..1123)
                     /locus_tag="OQM52_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1573..2985
                     /gene="ospC1"
                     /locus_tag="OQM52_003947"
     CDS             1573..2985
                     /gene="ospC1"
                     /locus_tag="OQM52_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCS1565586.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(3089..3238)
                     /locus_tag="OQM52_003948"
                     /pseudo
     CDS             complement(3089..3238)
                     /locus_tag="OQM52_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3314..5011
                     /gene="ospD3"
                     /locus_tag="OQM52_003949"
     CDS             3314..5011
                     /gene="ospD3"
                     /locus_tag="OQM52_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCS1565587.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1761 a          946 c          986 g         1450 t
ORIGIN      
        1 ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca tcggatggac
       61 gtgcacagct tgatcgtgat gcgtactgca accgtaatgt cgttgaacgg tgctttgggc
      121 ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga aattacctgg
      181 cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat taagggacac
      241 agcctagtct ccaggattcc cggggcggtt caatcaaagc gcagaacttc tgcgtcttct
      301 tcgtcaaaaa aatcatcttc gtgaagcttc acgacatagc ggggaagttt gcttctttga
      361 gaggcgggtt tacgtttacg ggatttagct gaacgggcca tataaccacc tgaaagacaa
      421 tgacattgcc tgtttttata acggtaattg cagaccatga caaaccgcag ccgtcaggct
      481 gcctactcga gtcacaaggc tctttgtgtc cgatatgccg gcagcgcatt acccatgaaa
      541 ctggctggaa cgtacaccat aaggtgaaga aagttatggg ggcggtgaag aattatctaa
      601 ccttgtcttg ttacatccaa attgccatcg acagttacac agttgtgaag ccggctctct
      661 caaaaagggc ttataaaggc ttgagcggtg taccgggaaa ccggtatgca ccgttcttag
      721 gggagggcag tccagcaatg aactgtcctt acccgacaaa catccaaaac gaacggaacg
      781 tgctggaatc cgcatataac ccgttgtaaa gtgaggaaat ggacacctca cttgctcatg
      841 agaacgcccg cctgcgggca ctgttgcaga cgcaacagga cactatccac cagatggctg
      901 aatacaaccg cctgctctca cagcggatgg cggcttatgc ttccgaaatc aaccgactga
      961 aggcgctggt tgcgaaactg cagcgtatgc agttcggtaa aagctcagaa aaacttcgcg
     1021 caaaaaccga acggcagatt ccattttcac gggcgatcta cgccacacag gctctgggtt
     1081 gcagtgactg ctccattaag gcgattttga actcataagg gaagtaacaa aaaaactgca
     1141 aaagtacaat aattatatat ggccaaacta acctctatta aaaaacatca gtttttcaca
     1201 cttagttgag cttcatgaaa aaatcatcga ggatttcatt gcagaataca cattccacta
     1261 actggaagca ctccataaga ggaatcagag aaagtatcgt ttttttatag taaaattcat
     1321 tgctgttcaa taaaattgat atagttatta aattatgaaa ataatttcac aaaaaagatt
     1381 aacttgagtt cttaagcatc ggattttaga tattttgaaa tatctggcat cacataacat
     1441 caaaagtata taatactctt atctaattac atgcagtata ttgtatatat actcaaaaat
     1501 tgtaattcat accctgctat atcttgtagg gaaagatatt aattaaaact gttttcatat
     1561 aaggttcatt ttatgaatat atcagaaaca ctgaactcag caaataccca atgcaatata
     1621 gattctatgg ataacagatt acatacattg tttccaaaag tgacatcagt gcgaaacgct
     1681 gcacaacaaa ctatgccaga tgaaaaaaat ttaaaagata gtgcaaatat tattaaaagt
     1741 ttctttagga aaactatagc agcacagagt tatagtagaa tgttctctca aggctctaac
     1801 tttaaatctt taaatatagc aattgatgca ccatcagacg ctaaagcctc atttaaggct
     1861 attgagcacc ttgacagatt atcgaagcat tatatatctg aaataaggga aaaacttcat
     1921 cctctttctg cagaggaact caatttgctt tcgctaatta ttaattctga tttaatcttc
     1981 agacatcaaa gtaattctga tttgtctgat aaaattttaa acattaagtc attcaataaa
     2041 attcagtctg aaggaatatg cacaaaacga aacacatacg ctgatgatat aaaaaaaata
     2101 gctaatcatg actttgtgtt ttttggcgtt gaaatctcta accatcagaa aaaacacccc
     2161 ctgaatacaa aacatcacac tgttgatttt ggtgcaaatg cgtatatcat tgatcatgac
     2221 tctccatatg gatatatgac attaaccgat cactttgata atgctattcc acctgttttt
     2281 taccatgagc accaatcatt tttagataaa ttttcagagg ttaataaaga agttagtcga
     2341 tacgtacatg gaagtaaagg aattatagat gtaccaatat tcaatactaa agatatgaag
     2401 ttagggctcg gattatacct gattgacttt attagaaaaa gtgaagacca aagcttcaag
     2461 gagttttgct atggaaaaaa tcttgcccct gtggatctgg atagaatcat aaactttgtt
     2521 tttcagccag agtaccatat acctaggatg gtaagtacag aaaacttcaa aaaagttaag
     2581 attagagaaa tatccttaga ggaggctgtt acagcatcta attacgaaga aattaacaag
     2641 caggtcacta acaaaaaaat tgctctccag gctctttttc tttcgattac taatcaaaaa
     2701 gaggatgtcg ccttatatat attatctaat tttgagataa ctagacaaga tgttatttcc
     2761 ataaagcatg agttgtatga tattgagtat ctacttagcg ctcataattc aagctgtaaa
     2821 gtacttgagt attttatcaa taagggattg gttgatgtaa acacaaagtt caaaaaaact
     2881 aatagtgggg attgtatgtt ggataacgca ataaaatatg agaatgcaga aatgataaaa
     2941 ctattattga aatatggtgc aacatctgac aataaatata tttaatcaaa attgaatatc
     3001 gtttagcaaa aggcagtatt tgcacaaaat atcgatatat tgtttattgt cagtatggct
     3061 caatgtgata atagttggaa agtttgatgg gtttcgcccc gttgtagcgg tagtcgaact
     3121 ggaaggtctt caggcactgc ttacagcgat agagcagccc ccctgaactg gaatggccgt
     3181 tccggatacc cccctgagtt tcagagtaac ggtgacaaac cacatcaatc tttgccatca
     3241 atcatccaaa gggcaaagag tacaacaaca ctaagtctgc gtcacaaccc atcaatgaaa
     3301 ggaatatata catatgccat cagtaaattt aatcccatca aggaaaatat gtttgcaaaa
     3361 tatgataaat aaagacaacg tctctgttga gacaatccag tctctattgc actcaaaaca
     3421 attgccatat ttttctgaca agaggagttt tttattaaat ctaaattgcc aagttaccga
     3481 tcactctgga agacttattg tctgtcgaca tttagcttcc tactggatag cacagtttaa
     3541 caaaagtagt ggtcacgtgg attatcatca ctttgctttt ccggatgaaa ttaaaaatta
     3601 tgtttcagtg agtgaagaag aaaaggctat taatgtgcct gctattattt attttgttga
     3661 aaacggttca tggggagata ttatttttta tattttcaat gaaatgattt ttcattccga
     3721 aaaaagcaga gcactagaaa taagtacatc aaatcacaat atggcattag gcttgaagat
     3781 taaagaaact aaaaatgggg gggattttgt cattcagctt tatgatccca accatacagc
     3841 aactcattta cgagcagagt ttaacaaatt taacttagct aaaataaaaa aactgactgt
     3901 agataatttt cttgatgaaa aacatcagaa atgttatggt cttatatccg acggtatgtc
     3961 tatatttgtg gacagacata ctccaacaag catgtcctcc ataatcagat ggcctgataa
     4021 tttacttcac cccaaagtta tttatcacgc gatgcgtatg ggattgactg agctaatcca
     4081 aaaagtaaca agagtcgtac aactatctga cctttcagac aatacgttag aattactttt
     4141 ggcagccaaa aatgacgatg gtttgtcagg attgctttta gctttacaaa atgggcattc
     4201 agatacaatc ttagcatacg gagaactctt ggaaacttct ggacttaacc ttgataaaac
     4261 ggtagaacta ctaactgcgg aaggaatggg aggacgaata tcgggtttat cccaagcact
     4321 tcaaaatggg catgcagaaa ctatcaaaac atacggaagg cttctcaaga agagagcaat
     4381 aaatatcgaa tacaataagc tgaaaaattt gctgaccgct tattattatg atgaagtaca
     4441 cagacagata cccggactaa tgtttgctct tcaaaatgga catgcagatg ctatacgcgc
     4501 atacggtgag ctcattctta gcccccctct cctcaactca gaggatattg taaatttgct
     4561 ggcctcaagg agatatgaca atgttcccgg acttctgtta gcattgaata atggacaggc
     4621 tgatgcaatc ttagcttatg gtgatatctt gaatgaggca aaacttaact tggataaaaa
     4681 agcagagctg ttagaagcga aagattctaa tggtttatct ggattgtttg tagccttgca
     4741 taatggatgt gtagaaacaa ttattgctta tgggaaaata cttcacactg cagaccttac
     4801 tccacatcag gcatcaaaat tactggcagc agaaggccca aatggggtat ctggattaat
     4861 tatagctttt caaaatagga attttgaggc aataaaaact tatatggaaa taataaaaaa
     4921 tgaaaatatt acacctgaag aaatagcaga acacttggac aaaaaaaatg gaagtgattt
     4981 tctagaaatt atgaagaata taaaaagctg aatattatct cgtggtgata cactgaattt
     5041 gaggtaaccg gctcatttaa accgtctggt ctgttacctc cggttccaca aaaataatgt
     5101 ccatcatttt gaagtggaca ctatcgtatg gaataccgga cct
//
LOCUS       DAJVQR010000180         5141 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVQR010000180 DAJVQR010000000
VERSION     DAJVQR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            190..447
                     /locus_tag="OQM52_003950"
                     /pseudo
     CDS             190..447
                     /locus_tag="OQM52_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OQM52_003951"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OQM52_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1565588.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OQM52_003952"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OQM52_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1565589.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OQM52_003953"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OQM52_003953"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1565590.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OQM52_003954"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OQM52_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OQM52_003955"
     CDS             complement(3605..4420)
                     /locus_tag="OQM52_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1565591.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5141
                     /locus_tag="OQM52_003956"
     CDS             4517..>5141
                     /locus_tag="OQM52_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1565592.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDL"
BASE COUNT         1370 a         1172 c         1226 g         1373 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg g
//
LOCUS       DAJVQR010000181         5112 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVQR010000181 DAJVQR010000000
VERSION     DAJVQR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..475)
                     /locus_tag="OQM52_003957"
     CDS             complement(<1..475)
                     /locus_tag="OQM52_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565593.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDIN"
     gene            complement(543..2492)
                     /locus_tag="OQM52_003958"
     CDS             complement(543..2492)
                     /locus_tag="OQM52_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1565594.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2577..2912)
                     /locus_tag="OQM52_003959"
     CDS             complement(2577..2912)
                     /locus_tag="OQM52_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1565595.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2912..3268)
                     /locus_tag="OQM52_003960"
     CDS             complement(2912..3268)
                     /locus_tag="OQM52_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1565596.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3268..4764)
                     /locus_tag="OQM52_003961"
     CDS             complement(3268..4764)
                     /locus_tag="OQM52_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1565597.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4761..4922)
                     /locus_tag="OQM52_003962"
     CDS             complement(4761..4922)
                     /locus_tag="OQM52_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCS1565598.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4932..>5112)
                     /locus_tag="OQM52_003963"
     CDS             complement(4932..>5112)
                     /locus_tag="OQM52_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565599.1"
                     /translation="YQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGP
                     DGDIEHHLEMRFPQN"
BASE COUNT         1210 a         1493 c         1101 g         1308 t
ORIGIN      
        1 cattaatatc tttcatattc aatttgcaat tttcgtaggt cactcccgac tcgccattaa
       61 cattaactcc agctctgata ttcccgtttt cttttacatc tacaaaaaac tggcctcttg
      121 gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc aaaatcatca tctttatata
      181 ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt tgcaaaatca aatacaccta
      241 tacttttgcc gaattcataa gaagcttttc tcaatataat cgcaccgctg gcagaagggg
      301 atgggcacga tatcctcagt tgtctgaatc catctgtatc caagtcaacg tcattatctg
      361 cagatgtttt aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg
      421 cctgagcaca gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac
      481 aaactccatt gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga
      541 actcaccttt taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct
      601 gttttcggca aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt
      661 tcgctgcggg ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca
      721 ttcggggtgt agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg
      781 gtcatgtcat agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga
      841 ccaaatccac tgttcagtgc atcagccaca aaatttttaa caagccaggg atgttctttt
      901 tcaaactcct gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg
      961 aggcccttta atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact
     1021 gttgccgcac gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct
     1081 attgttttta ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc
     1141 cagacttttt tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca
     1201 ccgacgagaa gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt
     1261 tttgttatgc gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt
     1321 ttcgcgatgg tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga
     1381 aaaggcttca tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg
     1441 gaagaaatcg cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca
     1501 cgggcggcat attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta
     1561 aagttggttc gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc
     1621 ttcccgaaca gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa
     1681 tccagtacgt gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc
     1741 gggctaatgc gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct
     1801 gtcagcgaca acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt
     1861 ccggcaatgg ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct
     1921 aaagggccaa tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtattg
     1981 ccaagatagt taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca
     2041 gtgcgccact gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc
     2101 atcttcacgg catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca
     2161 cccgccgcca caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg
     2221 cgcacctgct ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc
     2281 cggacatccg ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc
     2341 cccagtactg cggttccggc acccgctgcg cccagtgaca ggctggcaaa ctccttttta
     2401 aaacctttca gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg
     2461 atgatcgcct tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc
     2521 ctcggcttcc agttctgtaa actgcgaaac agccacccgt cgcagctcca gtggattcag
     2581 tttccagaac caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg
     2641 gtaaaaaaac gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc
     2701 gccgatgaac acgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc
     2761 agtttaatac tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca
     2821 tacgtgggct cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt
     2881 tttaaaacta tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga
     2941 attcaagatc tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg
     3001 ccgaaagcac atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg
     3061 tggtaatttt gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg
     3121 accgccaggt ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat
     3181 tggtgttcat cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa
     3241 aaaaacaggt tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa
     3301 ctggttaacc accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt
     3361 cagtcggttc gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta
     3421 tttttccaca ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat
     3481 aacggcagga gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt
     3541 gtgacgtccg tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt
     3601 atgcagagtt tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta
     3661 ggtggttacg gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc
     3721 gtgagataaa agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag
     3781 catccccacc agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc
     3841 ttcacgggca aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta
     3901 accggcaagc gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag
     3961 cgttcccagt tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc
     4021 gctgctgtca ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag
     4081 accgataaag tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc
     4141 tccgctaccg gcagcaccga cttccgtcac cacccgaagc cctgctggca aaatctcacc
     4201 accaccagaa ccataataat tcagacagac cggtaactca ttaccataca gccccttatg
     4261 acgggcagtc agcgtcacca cgcctgcatc agatgacgcc gtaaacggca gggtgataac
     4321 cccgtttacc gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac
     4381 aggcacctgt acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc
     4441 tccggcaacc gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac
     4501 atacagttca ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct
     4561 ccccgcccca caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac
     4621 ctcaatggct gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc
     4681 tgtatttgcc gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg
     4741 aacagtatta aaacttatcg tcatgatttt ttagccttat cctgtttaac acgtaccaca
     4801 tcacccgccg ctatacggcg gaaccagtag ctgctttctt ccacatttcg cccttcggaa
     4861 ggcaaaaggt ctccacgggc agggtcagga accgaccgcc ctttcagggg tttcacaaac
     4921 atgagggact cttaattctg agggaaacgc atttccagat ggtgctcaat gtcaccatcc
     4981 gggccagtac caggatcgat gtaatccaca tcaatactca gcagggataa atccggcagg
     5041 gcattcacgt cctctgcctg tcgcgtgtct tcttccgtga tttcatactt cgccgtaaaa
     5101 tcaaactggt aa
//
LOCUS       DAJVQR010000182         5091 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVQR010000182 DAJVQR010000000
VERSION     DAJVQR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..142
                     /locus_tag="OQM52_003964"
     CDS             <1..142
                     /locus_tag="OQM52_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1565600.1"
                     /translation="HGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQN
                     LA"
     gene            complement(252..1040)
                     /gene="phoH"
                     /locus_tag="OQM52_003965"
     CDS             complement(252..1040)
                     /gene="phoH"
                     /locus_tag="OQM52_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1565601.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1647..2912)
                     /gene="efeB"
                     /locus_tag="OQM52_003966"
     CDS             complement(1647..2912)
                     /gene="efeB"
                     /locus_tag="OQM52_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1565602.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2918..4045)
                     /gene="efeO"
                     /locus_tag="OQM52_003967"
     CDS             complement(2918..4045)
                     /gene="efeO"
                     /locus_tag="OQM52_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1565603.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4103..4777)
                     /locus_tag="OQM52_003968"
                     /pseudo
     CDS             complement(4103..4777)
                     /locus_tag="OQM52_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            4866..>5091
                     /locus_tag="OQM52_003969"
     CDS             4866..>5091
                     /locus_tag="OQM52_003969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565604.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRV"
BASE COUNT         1203 a         1402 c         1238 g         1248 t
ORIGIN      
        1 ccatggagaa cactttatca gccgggaaat aatgcgggca acggtgttta attatatcga
       61 atgtgattac aatcggtggc ggcggcacag ttggtgtggc ggcctcagtc cggaacaatt
      121 tgaaaaccag aacctcgctt aggcctgtgt ccatattacg tgggtaggat cagtttcaga
      181 aaaagcgcta gtccgggcat aaacacccaa aacaaagccc ggttcgcccg ggctctgcac
      241 cgataataca cttaactgta tgcatgcagc gtacgttggc aaagtgccga acgtacgcaa
      301 tcctcttttc cgaaccggac gatcccaacc atttcatctt cttcgaaacg ttccagcgcg
      361 tcacttaatc cggagcacac gccgcgaggc aaatcgcatt gcgtgatatc accgttgacg
      421 ataaccgtca cgttctcccc gaggcgggtt aaaaacattt tcatttgcgc ggcagtcaca
      481 ttctgcgcct cgtcaagaat gacaactgca ttttcaaagg tacgtccacg catataggcg
      541 aacggcgcgg tttccacctt ccctatttcc ggtcgcaggc agtactgcat aaaggaagcc
      601 cctaagcgcc ggaccagcaa gtcgtagacc gggcgaaaat agggagcaaa cttttctgcg
      661 atatcaccag gtaagaagcc aagatcttca tcggcttgca gaactggacg ggtgacgata
      721 atcctgtcga catccttatg tatcagggcc tctgccgctt ttgctgcgct gatccaggtt
      781 tttccgcacc cggcttcgcc cgtggcgaat atcagctgct tactctcaat agcctgcaga
      841 tagtgcaatt gcgcttcatt tcgcgcaagg atgggcgagg tatcgcgact gtcgcgggcc
      901 ataccaatgg cttctacgcc gctcatctgc acaagcgagg tgaccgattc ttcttcacgc
      961 tgcttatggc tgcgcgaatc ccgtctcagc acacgttttg cctcgcgacg agctttgatc
     1021 actgcttttt gtcttcccat ggagagcacc ttgagttgtt tgtattcatc acacgcgccg
     1081 ttggcagcgc gattatgcgc acgaacatca gagggttggc ttccttgtaa gccatagttt
     1141 gcttttggat aaaatgccga aaaacggcta cgcacaccgt ttgcggcgtc ggtaacacga
     1201 gtaaagaaag gaagtgagaa aaatacagag tgacgaagtt gctaccggag gagaaatctc
     1261 cgcgagtggt ggagcgttgc ttaacaaaac attgtccaga acaggacgtt accatccgcg
     1321 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     1381 agaactcgca ttcattgcaa caatatattc acttatattt aacgataaca cccatttcgg
     1441 tgacattaat ttctactgga aagatgacag agtgatgaca gtgatggaaa aagctgtgtg
     1501 ctttccgcag gattcgcagg gctggtaaaa tgtgcctcgt gtttgccgga ttcggcgcga
     1561 acgccttacc cgacctacaa gtgattaata atttgtgcac aaaactcatg cccgataaac
     1621 ctgcgcatcg ggcatatttg cgtcgttcaa acctgcaata gcgcccttcc cagataatcg
     1681 ttcgcgtcct tcactcccgg cagcgaaaaa aaataaccgc cgccgatagg tttaacgtat
     1741 tcctccagcg cttcgccatt gagccttttt tgtactgtca ggaagccttt ttccagatcg
     1801 tgttggtagc agacaaacag caaccccata tccagttgtc cggagttggt gacccccaac
     1861 gaataactgt agccgcgacg cagcatcagg ctggactcac tctccgccgt gcggggattc
     1921 gccagccgga tatggctgtc cagcgcgatc accttccctt ccgggtcgct ggcgtaatca
     1981 ggcacatcat gctcatgctg cattcccagc ggcgcaccgg tttgcttatc acggccaaaa
     2041 atcgtctgct gttctttcag cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag
     2101 cgtactgcct gatagctgcc accgattgtc cacgcaggct cctgctgatc tgccgttacc
     2161 cacaccactt tttgcatcaa cttatcattc tggctatcgg gattggcagt gccgtctttg
     2221 aaacccagca aattaatcgg cgtctctttg cctttactac gcgccgcgtg atcggaaata
     2281 aacccttccc gcttccagcg cacactgagc aaatccggcg tgtgtttgat gatatcgcgc
     2341 agcgcatggt tcaccgtgtc ctgggtgttg gcgcaaatct gtagcaacac atcaccatga
     2401 cataacgccg catccagcga gtcgttgggg aaacgcgtca tcttctgcag cttttttggc
     2461 atctgtggcg caaggccaaa gcgctcatca aacaatgagt gacccaccga taacgtgatg
     2521 gtgagattat cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc
     2581 ggatttggcg tttctggtgc tgctccgccc tgactcagaa aagcaaaacg ctgagtcaac
     2641 aagcgaaaca accgctcaag atcggcttta tcgctggcaa gcacatcaaa cgccaccagc
     2701 atcattgcgg cctgttgtgg cgtcaggatc cctgcctgat gctcaccata aaacggctgt
     2761 ttctcattgc gtgcatccgg tgaaagcgtg cccggtgcac tttgcgtttt ttgtgcatga
     2821 gcgaccggac aacttcccgc cagcgcacca atccctttca gtaaacgtcg gcgtgacggt
     2881 tcattcacgc cgttttcatc tttatactgc ataacgctta atccagtccc agcacaccgc
     2941 gaagttgcgc cagatcttcc gccagcgcag taatcggccc tttcagcgca ttccggtcgg
     3001 catcggtcaa tttgtcgtag gtttcaaaac cgtctttagt acggtatttc gccagaatgg
     3061 tatcgacctt tttaaagttg gcatcgactt ttgccaacag ttccgggttg gctttttgca
     3121 gttgtggacg cagcagatcg acaattttct gcgagccttc aacgttagcc tggaaatccc
     3181 acagatcggt gtggctgtag cgatcttctt caccgctgat tttgctggct gccacttcct
     3241 caatcagtcc ggctgcgccg ccgaccactt ttgaaggtgg gaaagccagt tcactgatgc
     3301 gtttttgcaa atcgaccaca tcggtataaa gctgctcagc gtactgatcc atccctttgg
     3361 tggtgttgtc gccaaacaat gctttttcca gacggtggaa accagtgaat tttgggtcgg
     3421 cggctttttg ctcgtaatca tcttcacggg cgtcaatgct gccatccaga tcggagaaca
     3481 gttcagcaat cggttcaata cgctcatagt gctggcgcgt cggcgcatac agtgctttcg
     3541 ctttttcgat atcgcctgct ttaatcgcgt cggtaaaggc tttggtgtcg gtcaccagct
     3601 gcgtggtttc cgccatgaca tacgctttat atgcagtaat tgcaccacca agacttaaca
     3661 gcgcatcact ttgcgccgca tccgccgttg cctcaccttt gacgatcaac ttccctttcg
     3721 ggttagtcag cagaccgcag gtcatatcgt attcgccagg ctgtaaattc gccgtcattt
     3781 tctggctaaa accaggggcg atattttccc gctcttccac caccatcacg cctttgagga
     3841 tctcccactc cagcgccttc tggctatggt tctgaataat gaactgtgtt ttcccggcgt
     3901 taaccgtaat ggtcatcggt tcgcactgct tatccgtcac ggtcactttg acctgcggca
     3961 catcagcggc gttagccata aaagcagaag aaaacagcgc agccacgctc aactgcaatg
     4021 cgttacggcg gaagttaatg gtcatgacga gactatccct ttaaagtatg ttgtaactaa
     4081 gcaagagttt gcgtcgtatt tgttacgcgg agcgagacgc tgtcgcccct gcgcgtggtg
     4141 gcagagcaaa tgccaccagc gccgggatga gataaataaa ccagacggcg acttcgctga
     4201 cgctcggtgc ttcctgatag ccaaaaatcc cttccatcag cgtgccaaac agcgagtgag
     4261 ttgagaggac cgcgctcata tcgaaggcga tttcctgaaa gtggttccac aatccggctt
     4321 catgaaatgc gcgaatggca ccagccgcca gccctgcggc gacgaagaga ataaacaggc
     4381 tggtccattt aaaaaatgca ccaagattga ggcgaatacc gccccagtag agcaggaagc
     4441 cgagcaccac ggcagtagca agaccgagca ttgcacccag cggcggccag atcccgacat
     4501 cttgttgaaa tgccgccagc aggaaaaaga ccgactccag cccttccctt gcaacggcaa
     4561 aaaagaccat catcaccagc gcccagccat gatgatttcc acgctgcaat gcgctatcga
     4621 ctgcctgttc cagttgcact ttgacgttgc gcgacacttt gcgcatccag aaaaccatcc
     4681 aggtaaggat caccacggcg atcaccgcca cgataccttc aaacagttcc tgctcttttt
     4741 gcggaaattc gccggtggtt tcgttaatga agatccccga gtaggcagcc tgacggctgc
     4801 ggcttgtcat ggtctgcaat taccgttata aaaacaggca atgtcattgt ctttcaggtg
     4861 gttatatggc ccgttcagct aaaccccgta aacgtaaacc cgcctctcaa agaagcaaac
     4921 ttccccgcta tgtcgtgaag cttcacgaag atgatttttt tgacgaagaa gacgcagaag
     4981 ttctgcgctt tgatagtttt gacgatgccg ttgaatgctg cgcagacctg aatattccgt
     5041 tctttgtgga tgcgggaaac aaaaagctgg tcttctggtt tgtacgtgtt g
//
LOCUS       DAJVQR010000183         5049 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVQR010000183 DAJVQR010000000
VERSION     DAJVQR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..1404)
                     /locus_tag="OQM52_003970"
     CDS             complement(<1..1404)
                     /locus_tag="OQM52_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCS1565605.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDA"
     gene            complement(1420..2304)
                     /locus_tag="OQM52_003971"
     CDS             complement(1420..2304)
                     /locus_tag="OQM52_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1565606.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2315..2920)
                     /locus_tag="OQM52_003972"
     CDS             complement(2315..2920)
                     /locus_tag="OQM52_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1565607.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3091..>5049)
                     /locus_tag="OQM52_003973"
     CDS             complement(3091..>5049)
                     /locus_tag="OQM52_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCS1565608.1"
                     /translation="TATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGV
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1465 a         1259 c         1042 g         1283 t
ORIGIN      
        1 cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg
       61 cccctgtgca acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc
      121 gtttgccagc gcatccagta catagcgtcc atgccctgcg gcaatgtcaa ccacgcggac
      181 ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcaatattt gcaaatgggt
      241 tttgcgctgg cgaataccgc gccatccaac actgttcagg tagattttgt cgattaaacg
      301 cccgaatgcg ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga
      361 atcaaagccg gtactcattc cgagatgcag gcccgcagag tgcgcaccga acaatttcat
      421 cgcctttcgc ataaagcgat acgctaaatc aaccggcgat aatggcacgg gtccaccaga
      481 aaggagccgc catcgatccg ctgatggtcc tgtgcggtct tcatgctgat aatcaaattt
      541 ttgcgatttg ttagcatata agcggcagat aaagctttgc attttttcaa atgcctgcgc
      601 cctgttctct tcacccaatg tgtcgtgata aaagcctggc agcagatgca gctctttcag
      661 agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc
      721 acctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc
      781 agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc
      841 attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgccctt tcacatagga
      901 attaataaaa aatagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg
      961 cacatacaat ttaaccttaa aggccggaga agccagcacc agcccacgaa ttgcaggtgc
     1021 ataatcatga acccatgtgg caacaagcac tgcgccgacg ctttgcgcga tcactaccac
     1081 ctcttccagt ccgacctggc tgtcgctggc agcaaaacgg acaaattcat cgacatcctg
     1141 cactgagcgc ataagagacg ggctataacc acgcggcccc gaattttttc catgcccccg
     1201 ggcatcccag gcataaaaag cagtatcagg catcgccagt tcatcaacga gatgttgtag
     1261 acgaccagaa tgttcatgcc cgcgatgaaa taagacgatg acctttttcg cccctggctg
     1321 taaagtgggc cagtgccgat aatacaatgc tgtattatca ctggtggtaa aaaaatgttc
     1381 cccagggatg cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat
     1441 aaaataaaaa aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc
     1501 atgtcctagt aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat
     1561 cactacatcg ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa
     1621 tgtatttagc ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac
     1681 gccccccagc agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct
     1741 gccgcaggat ttcccccata aatactgtgc aatatcgttt gactcggtta acgccaaaag
     1801 aaataacacc agtaacgccc cgccctggat atttatggtt ggcaacatta atagccaggc
     1861 ggcatgactc aaggcaaaaa cggtcattat ccatccccaa aaaatggcgc tcacggtatg
     1921 ctgaaaaccg gaggggtcac ccacaaaaac tcgccaggtg gctaatatca gaaacccagt
     1981 caaaggtata aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag
     2041 aggaataacc caatataacc aacgcgaaaa atgtacggat ataagcgtac aatattcttt
     2101 caatgccaga aaacttatca gcgtaaaaaa cgtcaacgtc atccagcgtg ggcctgaaat
     2161 caccagtgaa aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc
     2221 cccttttctc tcaggtaacc gccatagcag aaaacggttt atcagcttgc taaaattaac
     2281 aacgcgaaaa gtgttgccag agatttttcc agcattatct ttcagcgctc ataagaagca
     2341 cactccgaca acgattaatc gcagtacaga taagcagaat tgaagcaatg ctccataaaa
     2401 gattattcca ttgcatccac tgtggataaa tggcaacggc cagaccccac agaccaaata
     2461 ttaacgcgcg atcacttttg ccaaatggac cggcataact gcgaacaccg ttaatcgtct
     2521 gcgcgagtaa accgcaaaac tcggtcaata tggtgcaaaa taacatgagt atcacgagtg
     2581 atgcgttact ttccggtaaa aataaaaacg gtaaatagag cgcaatatcg gaaataacat
     2641 cgccagtctc attcaaaatc gcccccagac gtgtttgctg attgcactca cgcgccaaca
     2701 tgccatccag cgcattgagc gccatacgga taaaaagcac gatgggcaat agcaaaaaga
     2761 ggatgggttg tgccaccaac accaacagca atccggtaaa aagagaaagc gccagtgccg
     2821 taagagtgat gtgattcgct gtaacgtggt gcttataaag ccaaaacatc gtcggcctta
     2881 acagcgactg aaagagcggt tttatctggt agagcgtcat tatcaatcct tgataattcg
     2941 gtttgcgcta tggtgcatca attcccctga cggagatatc gcaacacgcc gcttcattaa
     3001 aaattcagaa tatatcaagg tattgggggc tcactgtcag gaatgattat ccccaataaa
     3061 tcaagtagtt aaaaattatt acgctgttat tcaatacaac tcatcaagtt tcctcatcgc
     3121 ttgttcccgt attgcgcgcg tcttaccgtt aagcgccagc tggcggacta aatccagttc
     3181 agggttaaca agctctaacg ccagcaaagg atcacgtttc agcctcgcct tcaatacatc
     3241 aactggaaag cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc
     3301 cagtgccgca gagggggtgt ggcggttgct aagtgcgccg aacttgaaaa atgtgagatt
     3361 gcttttcgcc agtgtactcc accatatacg atccggttgt tttgccagta catccagttg
     3421 tgctaacgca tcggggtcga gtgcataata aacacgtaat gcttcatcaa cctgcgcacg
     3481 ggagctattt tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcgtgttttg
     3541 ctgtaattcc ttgagaacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa
     3601 aaagtcccag tcttccaaac gtatcagggc ggcggcattt aatagcatct ggtcatcaac
     3661 ttcgccgtgg ctaatcagct cagacaaaat catatcagcg ctggtttcat atgcatctga
     3721 atctgctttc ggattatttt tccgatgcga taaagcacgt tgccaaattt tttgtttcat
     3781 cgacgtgctc agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg
     3841 tgtcaataac cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc gacagcaaaa
     3901 gtaggcaggg gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa
     3961 tgctaaaaat aacacacgta ttagatcatc gtcattctcg aatttttggt ataaataggc
     4021 tgcaactttg tcactctctt gtggcgtcat ccgcaataat tgaccatggt gtttcatctc
     4081 tgccagcttg aacgctaagc ttgtgacaac attgtttgat gaatcgttcg ccaactgcat
     4141 tgcctgattg taagtgagtt gtcctccagc accgacctga cgaatagagg aatcatcact
     4201 gcggatatag ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg
     4261 aatgatcata tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc
     4321 acgtagcgca agattcaacg gaccgacacc atcaggtaga cgactcattc gcgtcattat
     4381 ttcgacagag agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg
     4441 ctgatcggtc aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt
     4501 ttgttcatca ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct
     4561 tgggagagtt cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc
     4621 aacgttgcct tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg
     4681 aaacagcgct tcttttttca tctgattaca gacattccct gccatgcgaa tattgcttcg
     4741 cttaaaaatt tctttcagag catcttcatc atttattaga ccccaataat atgcatcagg
     4801 atttattatc atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac
     4861 catgaattca ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatac
     4921 cccacgcgca ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa
     4981 taccagtcca cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt
     5041 cgttgccgt
//
LOCUS       DAJVQR010000184         5022 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVQR010000184 DAJVQR010000000
VERSION     DAJVQR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OQM52_003974"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OQM52_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCS1565609.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OQM52_003975"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OQM52_003975"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCS1565610.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OQM52_003976"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OQM52_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCS1565611.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OQM52_003977"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OQM52_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1565612.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5022
                     /gene="yidZ"
                     /locus_tag="OQM52_003978"
     CDS             4378..>5022
                     /gene="yidZ"
                     /locus_tag="OQM52_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1565613.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1255 g         1415 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cc
//
LOCUS       DAJVQR010000185         4921 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVQR010000185 DAJVQR010000000
VERSION     DAJVQR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(187..771)
                     /gene="kptA"
                     /locus_tag="OQM52_003979"
     CDS             complement(187..771)
                     /gene="kptA"
                     /locus_tag="OQM52_003979"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCS1565614.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1021..1704
                     /locus_tag="OQM52_003980"
     CDS             1021..1704
                     /locus_tag="OQM52_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCS1565615.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1701..2162
                     /locus_tag="OQM52_003981"
     CDS             1701..2162
                     /locus_tag="OQM52_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1565616.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2175..3347
                     /gene="iadA"
                     /locus_tag="OQM52_003982"
     CDS             2175..3347
                     /gene="iadA"
                     /locus_tag="OQM52_003982"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1565617.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3412..4323
                     /gene="hypT"
                     /locus_tag="OQM52_003983"
                     /gene_synonym="qseD"
     CDS             3412..4323
                     /gene="hypT"
                     /locus_tag="OQM52_003983"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1565618.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4316..4708)
                     /gene="iraD"
                     /locus_tag="OQM52_003984"
     CDS             complement(4316..4708)
                     /gene="iraD"
                     /locus_tag="OQM52_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1565619.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4705..4788)
                     /gene="idlP"
                     /locus_tag="OQM52_003985"
     CDS             complement(4705..4788)
                     /gene="idlP"
                     /locus_tag="OQM52_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1565620.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1153 a         1295 c         1234 g         1239 t
ORIGIN      
        1 ggtatgaccg gaagcgacgc tgattccagt ccccaactga ccgcctgccg taaatcactt
       61 taatttcagc agttgttatt aaaggatacc cttttacgag ggtatccctt cagactgata
      121 acaaacgcta cattgccgga tgtgctgcgc ttaccaggcc tatataaccc ctgcaatata
      181 ttgaatttat tgtttttgta ccccagtcgg cataaacgac cactcaagaa actcaacagc
      241 aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc
      301 catttcctgt gctttgacgg ttaagacaac cggcgatccg tgtcttgctc ccactttgcg
      361 tgccgttgct tcatcagcag agagatgcac gtaatgacga tcccctgcaa tcagcccctg
      421 tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt
      481 tttttcggca aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca
      541 cctaccatca ctggaataac taaaacgctt tttatcgctg gttgcgacca cggtatccag
      601 caatgcacgg gttaatcgct tgcccgcttt ttgggcacaa agtatgagct tattgatatc
      661 cgcccatcct tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa
      721 acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca tcgcgttttc
      781 gtccctggat tttctgtttt tatttactaa tgtcattgtc actgggttta tgaattttct
      841 gcaactcaat cctgcgcaga accttttcca cacagcgtaa tttttcccct atgcaaaaaa
      901 cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca
      961 gttccaggaa ttttcctgac tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt
     1021 atgacacaac aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa
     1081 gggtatctgg ctttttttct caccataatc ttttttcccg gtgttttttc cggaacagac
     1141 agctggtggc gcgtttttga tttttccgtt ctgaacggat cgtttgggca actgcctggt
     1201 gcgaatggag ccaccacctc atttcgcggg gtgggtggcg cgggggctaa agatggcttt
     1261 ctctttgcgc tggagctggc accgtcagtc attctctctc tggggattat ttcaattacc
     1321 gatggtcttg gcggattacg cgccgcgcag cagttaatga cacctgtgct aaaaccgttg
     1381 ttgggcattc cagggatctg ctcgctggca ctgatcgcta acctgcaaaa cacggatgcc
     1441 gccgccggta tgaccaaaga gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg
     1501 attttcgccg catatcaaac cagcggtagc gccatcatca ccaactactt ctcttccggc
     1561 gtcgcggtgt ttgcctttct gggcacgtcc gtaatcgtac ctttagccgt catcctggtg
     1621 tttaaatttg tcggagccaa cattttgcgc gtctggctta acttcgaaga acgtcgcaac
     1681 ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg gatatgttta
     1741 tcgatggcgc gcgccgtggc tttactatcg ccacgaccaa cctgctgcca aacgtggtga
     1801 tggcgtttgt catcattcag gcgctgaaaa ttaccggcct gctcgactgg gtggggcata
     1861 tctgtgaacc cgtaatggcg ctgtggggat tacccggtga agcggcaacc gtccttttag
     1921 ccgcattaat gagtatgggc ggcgcagtcg gtgttgccgc cagtctggca accgctgacg
     1981 cattaaccgg ccacgatgtc accgttttac tgcccgcaat gtatttaatg ggcaacccgg
     2041 tgcaaaatgt cggtcgctgc ctggggacag ccgaagtgaa tgccaaatat tacccgcata
     2101 tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt
     2161 aacaaggagt taccatgatt gattataccg cagccggttt taccctgctg cagggagcgc
     2221 atttgtatgc gccggaagat cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta
     2281 tcgccgttgc cagcaatatc ccttctgaca ttgtaccgaa ctgcacggtt gtcgatctca
     2341 gcgggcagat cctctgccca ggttttattg atcaacacgt ccatttgatt ggcggtggcg
     2401 gcgaagcagg tcccacgacg cgcacgccgg aagtggcgct aagtcgcctg acggaagcgg
     2461 gcgtcacgtc agtggttggt ctgctgggca ccgactctat ctctcgccac ccggaatccc
     2521 tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg
     2581 cttatcatgt cccttcccgc actattacgg gttccgtgga aaaagacgtg gcgattatcg
     2641 atcgtgtgat tggcgtgaaa tgcgccatct ctgatcaccg ttctgccgca ccggacgttt
     2701 atcacctggc taatatggcg gcagaatccc gcgttggcgg tttgctcggc ggtaaacctg
     2761 gcgtcaccgt gttccacatg ggcgacagta aaaaggcgtt aaagcctatt tatgacctgc
     2821 tggaaaactg cgatgtgccg atcagcaagc tgctgccgac ccacgttaac cgcaacgtac
     2881 cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg caccatcgat atcaccagca
     2941 gcattgacga accggtcgcc cctgccgaag gtattgcccg cgccgttcag gcgggtattc
     3001 cgctggcacg cgtcaccctc agctccgacg gcaacggtag ccagccgttc ttcgatgacg
     3061 aagggaattt aacccatatc ggtgttgcag gttttgaaac gttgctggaa accgtgcagg
     3121 tgctggtcaa agactatgat ttcagtatca gcgatgccct gcgcccgctc accagtagcg
     3181 tagccggttt ccttaacatg agcgggaaag gcgaaattct gccaggcaat gacgcagact
     3241 tactggtcat gacgccagaa ttgcgcattg agcaggtata cgctcgcggc aaactgatgg
     3301 tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc
     3361 gccctaatgg acgtagaatg cccccaaggg cggctgacag agtaaaacgt aatggatgac
     3421 tgtggtgcgg ttttgcataa tattgaaacc aaatggcttt atgattttct gactctggaa
     3481 aaatgccgca atttttccca ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc
     3541 cgccgcatcc gtgcactgga acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg
     3601 ccactgcaac tctcagaaca agggaaaatc ttccattcgc agatccgcca tctgttgcaa
     3661 cagttagaaa gcaaccttgc agagctgcgt ggcggcagcg attacgcgca acgtaaaatc
     3721 aagatagccg ctgcacactc tctttccctc ggtctgttac cgtccattat cagccagatg
     3781 ccgccgctct ttacctgggc aattgaagcc attgatgtcg atgaagcggt cgataaactg
     3841 cgtgaagggc aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcgccg
     3901 tttgaccaca ttcgcttatt tgaatctcaa ttgttccctg tctgcgccag tgacgaacac
     3961 ggagaagcac tttttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac
     4021 tcctacatgg tgcgattgat taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc
     4081 accttttttg tctcttcgat gagcgagctt ttaaagcagg ttgccctcga cggctgtggg
     4141 attgcctggc tgccggagta tgccatccaa caagaaattc gcagcgggca gctcgttgtg
     4201 ctgaatcggg acgaacaggt gatcccgatt caggcctacg cataccggat gaatacccgc
     4261 atgaatccag ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgtgcttagc
     4321 tgacattctc aagcgtcgca ctgcgccaac cgccattatc ccagaccaga tcaaacgtta
     4381 gcggttcatc gtcgtggtac agcattatct caaggctgat cgccagagtg tggcagtacg
     4441 cttctttcaa attaacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac
     4501 gcagctgacg gcaataccag ctctggagat gtttgtcgct cgggttatgc acttgccact
     4561 cgggcatgcc tgaaacaagt aatgacggta atgccgaatg aggagaatta aaaagagtga
     4621 ggagatcgct gcataccgat ttacgcagca atgacgtttt attgcctgaa atttcaggta
     4681 aaacagcctg aagtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc
     4741 ggcttgccct gaacaccggg caccactgta ttgatgctca ttttccatag ccattccctt
     4801 ttgttcatta catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa
     4861 ccttacttgc ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa
     4921 t
//
LOCUS       DAJVQR010000186         4910 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVQR010000186 DAJVQR010000000
VERSION     DAJVQR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..484
                     /locus_tag="OQM52_003986"
     CDS             <1..484
                     /locus_tag="OQM52_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1565621.1"
                     /translation="AGEVIARIHMYKIATLGSGNPRNFTWNIISNNSVVMPTGGCTVD
                     SRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGV
                     GVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE
                     "
     gene            818..3097
                     /gene="ydeP"
                     /locus_tag="OQM52_003987"
     CDS             818..3097
                     /gene="ydeP"
                     /locus_tag="OQM52_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1565622.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3345..3548
                     /gene="safA"
                     /locus_tag="OQM52_003988"
     CDS             3345..3548
                     /gene="safA"
                     /locus_tag="OQM52_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1565623.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3616..4377
                     /gene="ydeO"
                     /locus_tag="OQM52_003989"
     CDS             3616..4377
                     /gene="ydeO"
                     /locus_tag="OQM52_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1565624.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4779..>4910
                     /locus_tag="OQM52_003990"
     CDS             4779..>4910
                     /locus_tag="OQM52_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1565625.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     "
BASE COUNT         1406 a         1040 c         1095 g         1369 t
ORIGIN      
        1 agcgggggaa gtcattgccc gtattcatat gtataaaatt gcaacattag gaagcggaaa
       61 tccgcgtaat tttacgtgga atattatttc aaataacagt gttgttatgc caacaggcgg
      121 ctgcaccgtt gatagtcgta atgtcaccgt ggatctcccg gactttcctg gaagcgcaga
      181 aatacccctt ggcgtatatt gctcaagcga acaaaagctc tcattttatc tttcgggtgc
      241 caccactgac agtagccgac aagtgtttgc taacacagca cctgatgcaa caaaagcatc
      301 gggagtgggt gtcactctaa tgcgtaatgg aaaaatactg gctacgggag aaaatgtctc
      361 cctggggaca gtaaataaaa gcaaagtacc tctgggttta agcgcaacct atggacaaac
      421 aggaaacaag gtttcagccg gaaccgtcca gtcagtcatt ggtgtcactt ttatctatga
      481 ataattgtga ttttgacctt agtctatgcg ctccattcac gaaaattgcg cggataatta
      541 ttttgtgaag gctattatcc tacacctgta agaaaatccg cgcacaattg aagccagagt
      601 cttatttttt atattggaaa atatgttaat ttaaacacta tgagagatga tgcttaactt
      661 atcgtattta atctattgtt taacgataat agattaaaaa tagaaagaga gtagtccctt
      721 actcttatat atccatgttg gcgataatcc ctttgtatgt actgtgcatc atcgctatta
      781 caaatcctaa taattcattt ccacacagga taagtagatg aagaaaaaaa ttgaatccta
      841 ccagggtgct gcaggtggtt ggggtgctgt taaatccgta gcgaatgcag tacgtaagca
      901 gatggatata cgccatgatg ttattgccat gtttgacatg aataagccag agggctttga
      961 ctgtccgggt tgtgcatggc cagatcctaa gaacagtgcg tcattcgaca tttgtgaaaa
     1021 cggcgcaaaa gcaatcgctt gggaagtcac ggataagcag gtaaatgcct ctttctttgc
     1081 tgagaatacg gttcaatcat tacttacctg gggagatcac gagcttgagg ctgcggggcg
     1141 tctcactcag cctttgaaat atgatgccgt cagcgactgt tacaagccat taagctggca
     1201 acaagctttc gacgaaattg gcgcacgcct tcaaagctat agtgatccca atcaggttga
     1261 attctatact tcgggccgca cttccaatga agctgccttt ctttatcagc tttttgcccg
     1321 tgaatacggg agcaataact ttcccgactg ctccaacatg tgccatgaac cgacaagcgt
     1381 gggtttggca gcgagtatcg gtgtaggtaa agggaccgtg ttgctggaag actttgagaa
     1441 gtgcgattta gtcatttgca ttgggcataa ccctggtaca aaccaccctc gcatgctgac
     1501 ttcgttgcgc gctttagtga aacggggagc gaaaatgatc gccatcaatc ctctacagga
     1561 acgtggcctg gagcgattta ccgcaccgca aaacccgttt gaaatgctga cgaactctga
     1621 gactcagttg gccagtgcct actacaacgt gcgcattggt ggcgatatgg cgttgctcaa
     1681 ggggatgatg cgcctgttaa ttgagcgcga tgatgctgca agtgccgcag gtcggccctc
     1741 attgcttgat gacgaattta ttcaaacgca taccgtcggc tttgacgagc tacgccgtga
     1801 cgttctcaat tccgagtgga aagatatcga acgtatttct ggactaagtc agacacaaat
     1861 cgccgaactg gctgacgcat atgccgctgc cgaacgcacc attatctgtt acggaatggg
     1921 gatcactcag cacgaacatg gtacccagaa cgtgcagcaa ctggtcaatt tgctgttgat
     1981 gaaaggtaac attggcaagc ctggtgcggg tatctgccca ctacgtggtc actctaatgt
     2041 acagggcgac cgaaccgtcg gtatcaccga gaaaccgtct gcagagtttc tggctcgcct
     2101 gggtgagcgc tatggcttcc ccccacctca tgcacctgga catgctgcaa ttgccagcat
     2161 gcaagcaata tgtacggggc aggctcgagc attgatctgc atggggggca actttgcact
     2221 ggcaatgcca gatcgggaag cgagcgctgt accgttaacg caattagatt tggcggtaca
     2281 cgtagccact aagcttaacc gctctcatct gctgaccgca cggcatagct atattctgcc
     2341 ggttctgggg cgtagcgaga ttgatatgca aaaaagcggt gcgcaggcgg taaccgttga
     2401 ggattcaatg tcgatgattc atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct
     2461 gaaatcagag tgtgcagtgg tcgcgggaat cgcgcaggca gcactacccc agagcgtggt
     2521 agcctgggag tatctggtgg aagattatga tcgcattcgc aatgacattg aagctgtgct
     2581 gccagagttc gccgactata accagcgcat ccgtcatccc ggtggttttc acctgataaa
     2641 tgcagctgct gaaaggcgct ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa
     2701 agggctgtta gaagatccct cttcagcgtt taacagtaag ctggtcatgg cgacagtacg
     2761 cagccacgat cagtacaaca cgacgattta tggtatggat gatcgctatc gaggggtatt
     2821 cggtcaacga gatgtggtct ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa
     2881 cggcgaaaga gttaatctta ttgcgcttac gccagacggt aagcgcagct cacgccgcat
     2941 ggatagatta aaagtggtca tttaccctat ggctgaccgc tcactggtga cctattttcc
     3001 agaatcgaat cacatgctaa cacttgataa ccacgatcca ttaagtggta ttcctggcta
     3061 taaaagtatt ccggttgaat tagaaccatc aaattaatgc ctcttctcat ttcttctgct
     3121 gtcatccgca cagcagaaga attcctcatt gactattatt gcgcaatttg ctcacatgga
     3181 ttaaattaaa ctacatacta taagatataa acttctgcct acagctgtaa gaaactccgc
     3241 tcagtactga agcaccagtc ctatttcttc ttttctccag cctgttatat taagcatact
     3301 gattaacgac ttttaacgtt atccgctaaa taaacatatt tgaaatgcat gcgaccacag
     3361 tgaaaaacaa aatcacgcaa agagataact ataaagaaat catgtctgta attgtggttg
     3421 tcttattact gacacttacg ttgatagcca ttttttcggc aattgatcag ctgagtattt
     3481 cagaaatggg tcgcattgca agagatctta cacatttcat tatcaatagt ttgcaagact
     3541 ggaaataaac agcaaattat aaatgtgaaa tgtttaaaag gtatcgataa aactttattg
     3601 ttttaaggag ataaaatgtc gctcgtttgt tctgttatat ttattcatca tgctttcaac
     3661 gctaacattt tagataaaga ttacgccttc tccgacggcg agatcctgat ggtagataac
     3721 gctgttcgta cacattttga accttatgag cggcatttta aagaaatcgg atttaatgaa
     3781 aataccatta aaaaatatct acaatgcact aacatccaga cagtgacggt gcctgttcct
     3841 gcgaagtttt tacgtgcttc aaatgtaccg actggattgc ttaatgaaat gattgcttat
     3901 ctcaactcgg aagaacgcaa tcatcataat ttttcagaac ttttgctttt ttcttgcctg
     3961 tctatttttg ccgcatgcaa aggtttcatt acactattaa ctaacggtgt gctatccgtt
     4021 tccgggaaag tgagaaatat tgtcaacatg aagctggcgc acccatggaa gctgaaagat
     4081 atttgtgact gcctgtacat cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa
     4141 acgacattct cacagattct attagatgcc agaatgcagc acgcaaaaaa tttgatacgc
     4201 gtagaaggtt cagtcaataa aattgccgaa caatgtggtt atgccagtac atcttatttt
     4261 atttatgcgt tccgcaaaca tttcggcaac agtccgaaga gagtttctaa ggagtaccgt
     4321 tgtcaaaggc acacgggtat gaatacgggc aacacgatga gtgctttagc tatttgatta
     4381 tttgctaacg agtagtcaac cacgcacgct acgtaagaat tatatggggc agccattccc
     4441 tgccccgcgt tgtttttagg cgatatattt attgaaataa ataagtgaca tccatcacat
     4501 atttatgcac ttgcataacc tgttgcatga ttatttatga tctcaattcg gcattttgtc
     4561 agtaaaatgc aataatttat taaatatcaa taaattagtt gtttatcggc gagaaattac
     4621 ttaatagaac agaaagtaat gtcaacgctt tatggactgt tatttccctt tttttagcta
     4681 aatctgctat ctctttatgt gactaacttc acttacatcc acttatttct cttcgtaaaa
     4741 ttacttcgga attaagtaca ataagaagag gaacatttat gaagtctgca ttaaagaaaa
     4801 gtgtcgtaag tacctcgata tctttgatac tggcatctgg tatggctgca tttgctgctc
     4861 atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc
//
LOCUS       DAJVQR010000187         4776 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVQR010000187 DAJVQR010000000
VERSION     DAJVQR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..599
                     /locus_tag="OQM52_003991"
     CDS             <1..599
                     /locus_tag="OQM52_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCS1565626.1"
                     /translation="QLWWSVWLCWPVLSLLIIGRGCGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(653..1258)
                     /gene="azoR"
                     /locus_tag="OQM52_003992"
     CDS             complement(653..1258)
                     /gene="azoR"
                     /locus_tag="OQM52_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1565627.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1459..>4776
                     /gene="hrpA"
                     /locus_tag="OQM52_003993"
     CDS             1459..>4776
                     /gene="hrpA"
                     /locus_tag="OQM52_003993"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1565628.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVL"
BASE COUNT         1163 a         1200 c         1321 g         1092 t
ORIGIN      
        1 ttcagttatg gtggtcagtc tggttatgtt ggccagtatt atcgctactc attattggcc
       61 gtgggtgcgg tgggcttggc gcgataacaa cagggaaaga tagtcagggg aaactcccgc
      121 ccgccgttta ctggctgaca ttgccctggc gcatcgggat gtggctatct atgcgttggt
      181 tttgtcgtcg cctggagccg gtgagcaaaa tgactgctgg tgtttattta agggcgtttc
      241 cacgacatat tccggcacag aatgcggttc tggacgtcac ctttgaattc cctcgtggac
      301 gagcgacaaa agatcgactc tatttctgtg taccgatgtt ggatctggtg gttccggaag
      361 agggggagct ccgacaggcc gtggcgatgc tggaaacatt acgcgaagag caaggcagcg
      421 ttctggtcca ttgcgcattg ggattatcgc gcagtgcgct ggtagtggcg gcatggctgt
      481 tatgttacgg acactgtaaa acagttgatg aagcgattat ctttattcga gccagacgct
      541 cgcatattgt gcttaaggaa gagcacaaag cgatgttgaa attatgggaa aacaggtaag
      601 tggattgaga tgtggactga atatctacag tccacatcaa gaccgtgtcc ggttatgcag
      661 caacaatgct gtcgatggct gcttttgcgt cagactgtgc tttcgctgcc atttccggac
      721 cgtatgcgat cccttcggcg aagacaaatt tcacatcggt aatgccgata aagccgagga
      781 acgtggacag atacggcgtc accaggtccg ttggtccatc tttgtggatc ccaccgcggc
      841 tggtaataac gatggctttt ttacccgtta ccagaccttc cggaccgttc tcggtatagc
      901 ggaaagtaac gcctgcgcgt gcaaccaggt caaaatagtt tttcaactga gttgagatgt
      961 tgaagttata catcggtgcc gcaataacga taacgtcgtg ggctttcagc tcggcaatca
     1021 gctcatcgga aagcgccaga gcttcctgct gacgcggagt cagcggcgca tcgctcggac
     1081 gcagagcgcc aaccagttcg ccatccagta ccggaatcgg atttgcagct aggtcgcgaa
     1141 cggtgatttc atcagcggag tgcttttcgc tccattgttc aacaaaataa tcggacaact
     1201 gattagactg agagtaccct gccaggatgc tggatttaag aactaatacc ttgctcatgg
     1261 tgtttcctta tagatgtttg aatggacgat gccccgttgc ttgctgacac tttattcaca
     1321 atcctgccac agagatcgcg caataaatcg aagtctatgt tcgaatttat tgaacaacgc
     1381 atagaaagcc gcgatgtggt actctatatc tatcatttaa aagaaaatta atcaggcaga
     1441 ctactgccca ctaacgttat gacagaacaa caaaaattga cctttactgc cttgcaacag
     1501 cggctggatt cgctgatgct gcgcgacaga ctgcgttttt ctcgccgtct gcattgtgtt
     1561 aagaaggtta aaaatcctga tgcacaacag gccattttcc aggagatggc gaaagagatt
     1621 gaccaggcgg cagggaaagt cctgctgcgt gaagcggcac gaccggaaat cacttatcct
     1681 gacaatttac cggttagtca gaaaaaacag gacatactcg aagcgattcg tgatcaccag
     1741 gtggtgatcg tcgccgggga aaccggctcc ggtaaaacga ctcagctacc gaaaatctgt
     1801 atggagctgg ggcgcggtat taaagggctg atcggtcata cccagccgcg tcgcctggcg
     1861 gcacgaacgg tggccaaccg tattgcggaa gagctgaaaa cggagccggg cggttgcatc
     1921 ggttacaaag tgcgtttcag cgatcacgta agtgataaca cgatggtcaa gctgatgacc
     1981 gacgggatcc tgctggcgga gatccagcaa gaccgcctgc tgatgcagta cgacaccatc
     2041 attattgacg aagcgcacga acgcagcctg aatatcgatt ttctgctcgg ctatttgaaa
     2101 gagttgctgc cgcggcgtcc tgacctaaaa atcattatca cttccgcgac tatcgacccg
     2161 gaacgctttt cgcgccactt taataatgcg ccgattattg aagtctccgg tcggacctat
     2221 ccggtggaag tgcgctatcg cccgattgtt gaagaagccg atgacaccga gcgcgatcag
     2281 ttgcaggcga tttttgacgc cgtagacgaa ctaagccagg aaagccccgg cgacattctg
     2341 atctttatga gcggcgagcg ggaaattcgc gataccgccg atgcgctgaa caagctgaac
     2401 ttacgccata ccgaaatctt gccgctttat gcgcggcttt cgaacagcga acagaaccgc
     2461 gtgttccagt cgcacagcgg acggcgcatt gtgctggcga ccaacgtcgc ggaaacctcg
     2521 ctgaccgtac cggggattaa atacgtcatc gaccccggta cagcgcgtat cagccgctac
     2581 agctatcgca ccaaagtgca gcgtttgccg attgaaccca tttcgcaggc gtctgccaat
     2641 cagcgtaaag gccgctgtgg tcgtgtgtcc gaagggatct gtattcgtct ctattccgaa
     2701 gacgatttcc tctcgcgccc ggagtttacc gatccggaga ttctgcgtac caacctggcc
     2761 tcggttattt tgcagatgac cgcgctgggg ctgggcgata tcgctgcgtt cccgtttgtc
     2821 gaagcaccgg ataaacgcaa tatccaggat ggcgtgcgtc tgctcgaaga actgggcgcg
     2881 atcaccacgg atgaacaggc cagcgcctat aaactgacgc cgctcggtcg ccagctctcg
     2941 cagttgcctg tcgacccacg tctggcgcgt atggtgctgg aagcgcaaaa acatggctgc
     3001 gtgcgtgagg cgatgattat cacgtccgcg ctctccatcc aggatccgcg cgaacgtccg
     3061 atggacaaac agcaggcatc ggacgaaaaa catcgtcgct tccacgacaa agaatccgac
     3121 ttcctcgcgt ttgtaaatct gtggaattat cttggcgagc agcaaaaggc gctttcttcc
     3181 aacgccttcc gtcgcctgtg tcgtaccgat tacctcaact atctgcgcgt gcgcgaatgg
     3241 caggatatct acacccagtt gcgccaggtg gtgaaagaac ttggcattcc ggtcaacagc
     3301 gaaccggcgg agtatcgtga aattcacatc gccttactga ccggtttgct ttcccatatc
     3361 ggcatgaaag atgccgataa acaggaatat accggtgcac gtaacgcgcg tttctccatc
     3421 ttccccggtt ctggtttatt caaaaagccg ccgaaatggg taatggtggc ggaactggta
     3481 gaaaccagcc gcctgtgggg gcgcattgct gcgcgtatcg acccggaatg ggtagaaccc
     3541 gttgctcagc atttgattaa acgcacctac agcgaaccgc actgggaacg ggcgcagggc
     3601 gcggtgatgg caacggaaaa agtcactgtt tatggtttgc cgattgttgc tgcgcgcaag
     3661 gtcaactaca gccagatcga ttcggcgtta tgtcgtgaac tctttattcg ccacgctctg
     3721 gtggaaggtg actggcagac gcgtcacgca ttcttccgtg aaaacctgaa actgcgggcc
     3781 gaagtggaag agctggaaca caaatcacgt cgccgcgata ttctggttga tgacgaaacg
     3841 ctgtttgagt tctacgacca gcgcatcagc cacgatgtaa tctccgctcg tcacttcgat
     3901 agctggtgga aaaaagtcag ccgcgaaacg cctgatttgc tcaactttga aaagagcatg
     3961 ttgatcaaag aaggggcgga aaaaatcagc aagctggatt acccgaactt ctggcatcag
     4021 ggcaatctca agctgcgttt gagatatcag tttgagccag gcgcggacgc ggacggtgtg
     4081 accgtacata ttccgctgcc gttacttaac caggttgagg aaagcgggtt tgaatggcag
     4141 atccccggtc tgcgccgcga attggtgatt gctctgatta aatcgttgcc gaaaccggta
     4201 cgccgtaatt ttgtgcctgc accaaactac gccgaagcgt ttttaggccg cgtcacaccg
     4261 ctggagttac cgttgctcga cagccttgag cgcgagttac ggcggatgac cggcgttacc
     4321 gttgaccgcg aagactggca ctgggatcag gtgcccgatc acctgaaaat caccttccgc
     4381 gtggtggatg acaaaaacaa gaagctaaaa gaagggcgct cgctacaaga tctgaaagat
     4441 gcgctgaaag gcaaagtgca ggaaacgctg tccgcggtgg cggatgacgg tatcgagcag
     4501 agcggcttac atatctggag ttttggtcag ctgccggaaa gttacgaaca gaagcgtggc
     4561 aactataaag tgaaggcgtg gccggcgctg gtggatgaac gcgacagcgt gacgatcaaa
     4621 ctgtttgata acccgctgga gcaaaaacag gcaatgtgga atggtcttcg ccgtctactg
     4681 ctgctaaata tttcctcgcc gatcaagtat ttacatgaga agttaccaaa caaagccaag
     4741 ctggggctgt actttaaccc gtatggcaaa gtgctg
//
LOCUS       DAJVQR010000188         4611 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVQR010000188 DAJVQR010000000
VERSION     DAJVQR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OQM52_003994"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OQM52_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1565629.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OQM52_003995"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OQM52_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1565630.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OQM52_003996"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OQM52_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1565631.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OQM52_003997"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OQM52_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1565632.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4148..>4611)
                     /locus_tag="OQM52_003998"
     CDS             complement(4148..>4611)
                     /locus_tag="OQM52_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1565633.1"
                     /translation="LDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1229 a         1119 c         1100 g         1163 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta
     3961 gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt
     4021 cagatagttg tgatttttgc ccttacttgt tcaggttgta ttgttctttc tttgtaattt
     4081 gttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt gggggcaaac
     4141 tttgcaacta cgataaccgc gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa
     4201 tccacatacc gtaaatttca tacaccattt gcgcagtttc atgccccatc tggctggcta
     4261 taaatgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt cgcgtatggt
     4321 acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctt gtacccaaac
     4381 tgcttaccga gtaataaggt ttctgttttt cgttacacac cctgggcatg aaaacaaaat
     4441 gcagtttttg tttttcagtt ctgccgtact cccgatgata aaaagtgatt tcgcttttgc
     4501 gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aagagccggc tgcagtaatg
     4561 ctaccgtccg gatcccggca tttgtttttg ggggaccgaa catatcaagt a
//
LOCUS       DAJVQR010000189         4584 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVQR010000189 DAJVQR010000000
VERSION     DAJVQR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(177..506)
                     /locus_tag="OQM52_003999"
     CDS             complement(177..506)
                     /locus_tag="OQM52_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1565634.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(511..696)
                     /locus_tag="OQM52_004000"
     CDS             complement(511..696)
                     /locus_tag="OQM52_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1565635.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(693..3332)
                     /locus_tag="OQM52_004001"
     CDS             complement(693..3332)
                     /locus_tag="OQM52_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1565636.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3540..4529
                     /gene="ldhA"
                     /locus_tag="OQM52_004002"
     CDS             3540..4529
                     /gene="ldhA"
                     /locus_tag="OQM52_004002"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1565637.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT         1145 a         1202 c         1059 g         1178 t
ORIGIN      
        1 gctaactaat tggcgttgca tgtactgcaa cgccaattag ttaaaacaga tatttgacta
       61 tgcatttttc agggcgaaag gcaatttgac aggagttttt gaatattcac cttgtttcag
      121 gaaaatagat caatcgcttt ctcctgtagc gttatcccga tattgcggtt ttgctattaa
      181 aactttcgca cccatttacc gtttatccct tgtacatatt cccccggttt ggcccgagct
      241 acgagctttt gcccggcgag tttcgcgata tcatctacag acacattatt ttgctttgcc
      301 cgttgttggt aactcgcttt acgttcggca ttaatttcgg ttactaattt ctctgtttca
      361 gcatccgttt tcagcgcaac cagataacca taaaatgttt cacctacccg cccctgagtt
      421 ctggcttcat ccagagtcaa tgccattaca ttgctgctta ccagcccaac aagaaacgca
      481 caaagaagta atgttttctt catcatcgcc tcagaaaaga tcgctacgag tttcaagcag
      541 ctcttcgaca tctttgtctg ccttgatgat gatctcatgc tcaattttaa cgttcatatt
      601 gatagtgatc ggttccttag gtgcagcgac ttcaatgcga ggcgtacagc caaccagcat
      661 aaaagatgac gtcaacgcag ccagtaaaat tttcattgtt tttcctcaca ctcttttcct
      721 tgcggacagt catttcccgg cagacgtgcg ttctgctcca accatgcctg gagattatcg
      781 ccaaagcgta aactgcgcca cagcgtaaaa atattctctt catgatggta attaaggttt
      841 accgtaccgc ttttaccatc aacgcgactg gtacctgtaa tgttggcctg catggttaat
      901 aaaccgagat tatctaagtt aatttttgtc gatgaccggc taatttccat atagcgcaac
      961 cagttaattg ctgaacccgc agtcatattg tctttcacca ccgcatctgc ggtatcttta
     1021 tccagccgca aggtcattag cccgctattt gccagccaac catctttcac tatccatttt
     1081 tcgttgttta gccataacgg caatgcacca ctaaatgccc cggacatggc gaattgtttc
     1141 ggattgacgg cgctaaccag ttcgctggat gagagattat tcagccgcaa cagcgccggg
     1201 tcatgttgcg gcatacgtaa ttgtttcatc agcacgttac cgcctaacac atcgacgcta
     1261 acatcagtca acagcaaggg ttcttcttca gtccacggat aacgcccttg caaatcagcc
     1321 gtaatatttt tcgctgtcac cagattaatc acttcggcaa ttcgcaacgt aacggggccg
     1381 cgagtcccca gatgccaggc tccatcggca aaacggaaag gcaggacaaa atcgacaccg
     1441 ttaacctgat tatctggcat ccaggcacta ccgcctttca acacgccgtg ccctcccgcg
     1501 cggaatcctt gttcaggcgc agcagaaaat gcaacctgag catacagttc accatcgcgt
     1561 aagttcatct tccagtcggg tggcaccagc ggctggaata cagtcagtga ttgttttggc
     1621 caccaggcgt taccgcgcag acgaataccg tcccagcgac catttacccg aaccgggcca
     1681 atctcaccag catgtaaatc gcctttaaag aggaaatagg tcggattgcg cccatcgacg
     1741 ctaaatttta aggttgatgg cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg
     1801 tccagtgaca gcgcgccgct gaaggaggga tgctgtgcgt cacgtaccca gacgacgggc
     1861 ttgtcgagaa ttaatcgcgg cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa
     1921 ccggtggaga gatcggtcag cgtaatcgtg ctgtcatgcc actcaccttt tcctgcgaca
     1981 tcccaggtgg cattcatcgg tgtaaaactc ccttttcccc agtagcgcca ctgccagcgg
     2041 ccagcgtcag ggagaaaatc attcgccagc ccatccatat gcagcacgaa atcgcccagt
     2101 tcattttcat gcgcctgcaa aatggcctgc aaacgtccgt caacaccacg ttgggtgact
     2161 tttacacccg ctaaaggcca gcggatttca tcgatgtcca gcgaatcgat gactcttccc
     2221 tttgaacgaa gtaacgcgcc tggctcaaag gtcagcgttg ggtcagacag acttccactt
     2281 agctgcgcag gtaaacgggc atataaaatg aggtccgcct gtttcgcttc accggtcagc
     2341 tgcagaggca gttgactgtt atccatgctt aattttcctg ggccaaaatt aagcaccgcg
     2401 ttgcccttac ccgcttgccc ctgggtcagc acattcagtc gtccgctgac cagagcgttc
     2461 tcaagccctg cctgccagtt gtcgactttg acacccagtc ggccactcaa aggaaaacca
     2521 gcatacagcc agctccagcg accatcgctt acggtcaatt gttgacgagt aatttgccac
     2581 ggcaaatcga gcaacggatc gccgttatcc cgtgccagca cgatcaattg cccgctattt
     2641 tcctgccagt ccagctcggc atccaccagt gacggttcct gcggcaagtt taacgtcgca
     2701 gtagcatgcc cacttacagg aagtccatcc ggcacgagcg gcatagtaaa ttcccccacc
     2761 agtttaaccg gcggctgatt ttcaaacgcg acgacatcca gttcgctgac tgtaagttgt
     2821 tgccctttca gctggccttg aaatttaact ttttcgccct gataacgcag ttgctggata
     2881 tcagaggtta atgcgagaga gagttttccc tgccattcct gccagggaga aaaaatcagt
     2941 ttatcgatat tgatccaggt gttaggcagc atggactgcc actgcgcgag ggtttttgga
     3001 gcagctggcg attgctccgt ctgcggcaat ttcgccagac aagcagaatc aagttctacc
     3061 gtgccgacgt tcaataacca gcggctggga tgtgaaaggc tggcgttggt gatatgcgca
     3121 agctgacaat ctcccaccag ataacggaga tcgggaatga ttaaaccttt acgcgtaatg
     3181 cgtgggcttt catctaatgc aatacgtgtc ccgagcggta gccagatgtc cgccagcgtg
     3241 ggaacccaca gcccgagcgt catcagcagc gtcaacggca caagaataat cagtaataac
     3301 agcgcgagaa cggctttata tttacccagc atgggtagtt aatatcctga tttagcgaaa
     3361 aattaagcat tcaatacggg tattgtggca tgtttaaccg ttcagttgaa ggttgcgcct
     3421 acactaagca tagttgttga tgaatttttc aatatcgcca tggctttcaa ttatatttga
     3481 aattttgtaa aatattttta gtagcttaaa tgtgattcaa catcactgga gaaagtctta
     3541 tgaaactcgc cgtttatagc acaaaacagt acgacaagaa gtacctgcaa caggtgaacg
     3601 agtcctttgg ctttgagctg gaattttttg actttctgct gacggaaaaa accgctaaaa
     3661 ctgccaatgg ctgcgaagcg gtatgtattt tcgtaaacga tgacggcagc cacccggtgc
     3721 tggaagagct gaaaaagcac ggcgttaaat atatcgccct gcgctgtgcc ggtttcaata
     3781 acgtcgacct tgatgcggca aaagaactgg ggctgaaagt agtccgtgtt ccagcctatg
     3841 atccagaggc cgttgctgaa cacgccatcg gtatgatgat gacgctgaac cgccgtattc
     3901 accgcgcgta tcagcgtacc cgtgacgcta acttctctct ggaaggtctg accggcttta
     3961 ctatgtatgg caaaacggca ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc
     4021 gcattctgaa aggttttggt atgcgtctgc tggcgttcga tccgtatcca agtgcagcgg
     4081 cgctggaact cggtgtggag tatgtcgatc tgccaaccct gttctctgaa tcagacgtta
     4141 tctctctgca ctgcccgctg acaccggaaa actaccatct gttgaacgaa gccgccttcg
     4201 atcagatgaa aaatggcgtg atgatcgtca ataccagtcg cggtgcattg attgattctc
     4261 aggcggcaat tgaagcgctg aaaaatcaga aaattggttc gttgggtatg gacgtctatg
     4321 agaacgaacg cgatctgttc tttgaagata aatccaacga cgtgatccag gatgacgtat
     4381 tccgtcgcct gtctgcctgc cacaacgtgc tgtttaccgg gcaccaggca ttcctgacag
     4441 cagaagctct gaccagtatt tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag
     4501 gtgaaacctg cccgaacgaa ctggtttaat cttgcagctc ccctgcaatc caggggagct
     4561 gattcagata atccccaatg acct
//
LOCUS       DAJVQR010000190         4533 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVQR010000190 DAJVQR010000000
VERSION     DAJVQR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..1224
                     /locus_tag="OQM52_004003"
     CDS             <1..1224
                     /locus_tag="OQM52_004003"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1565638.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="OQM52_004004"
     CDS             1224..1958
                     /locus_tag="OQM52_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1565639.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2425..3186
                     /locus_tag="OQM52_004005"
     CDS             2425..3186
                     /locus_tag="OQM52_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1565640.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3230..4309
                     /locus_tag="OQM52_004006"
     CDS             3230..4309
                     /locus_tag="OQM52_004006"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1565641.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4306..>4533
                     /locus_tag="OQM52_004007"
     CDS             4306..>4533
                     /locus_tag="OQM52_004007"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1565642.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSIS"
BASE COUNT         1243 a          862 c         1117 g         1311 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtaggctt ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa
     2281 taacaaaatt aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag
     2341 agtaaggcgt tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc ggatttatca
     2401 gttgtcgaaa tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt
     2461 tgcttgtcgt ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact
     2521 caaaacactt cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg
     2581 tcggatggcg cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt
     2641 aaagtctttg aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc
     2701 aggcttaatc caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa
     2761 gacagggaaa aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt
     2821 tgggccgaga aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac
     2881 gtacatacct gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat
     2941 gcgagtgaga agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg
     3001 ccgttctaca tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat
     3061 tatattgcgc cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt
     3121 aaatggtctg tgattactga tgaaggtggt gagagtcgtt tgtttatatc tcatttgcaa
     3181 tcataatgta ttacattttt attgaatatt tttatgccat gcatattcca tgttttatcg
     3241 actgtcatta attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc
     3301 atttgattct tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca
     3361 gggagagcaa ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga
     3421 tgttggggag tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg
     3481 tttaaccgtt gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac
     3541 tgacaataaa aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta
     3601 tgattttgat agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa
     3661 aagtagttta gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta
     3721 tcagacaaat attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg
     3781 ttatggcagg ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac
     3841 atggagcaag gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg
     3901 aggcataaat gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt
     3961 gtttgacagc ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc
     4021 ggaggacagc tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat
     4081 tattaaacaa aatgggcaaa ttatttttac ccgttctgtg ccgccagggc cttttgcgct
     4141 ggataattta ccaacgctgg ccgttggcgg ggagcttgat gttacagtga gagagagtaa
     4201 tggcgaggag cagtattttt ccgtgccatt tcaaacgccg gccatcgctc tgcatgaagg
     4261 atatttcaaa tatagtgtga tgggggggaa tataaagaag aaggtataaa aagctcccct
     4321 gtctccgtgg gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac ggtgtatgga
     4381 gggattcaaa acgccactca ttttaatgca atatctacgg gtattggcat ttctctgggg
     4441 ctgttaggga gtttatcgac agatattacg cgttcgattg cgaatttata ttatgggaat
     4501 aaatatcgta ttcgttatag taaatccatt agt
//
LOCUS       DAJVQR010000191         4211 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVQR010000191 DAJVQR010000000
VERSION     DAJVQR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM52_004008"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM52_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1565643.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OQM52_004009"
     tRNA            complement(324..399)
                     /locus_tag="OQM52_004009"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OQM52_004010"
     tRNA            complement(406..480)
                     /locus_tag="OQM52_004010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OQM52_004011"
     tRNA            complement(597..681)
                     /locus_tag="OQM52_004011"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OQM52_004012"
     tRNA            complement(690..765)
                     /locus_tag="OQM52_004012"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OQM52_004013"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OQM52_004013"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1565644.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQM52_004014"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQM52_004014"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1565645.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQM52_004015"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQM52_004015"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1565646.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1047 c          937 g         1138 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggcttt c
//
LOCUS       DAJVQR010000192         4169 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVQR010000192 DAJVQR010000000
VERSION     DAJVQR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(344..991)
                     /gene="ychE"
                     /locus_tag="OQM52_004016"
     CDS             complement(344..991)
                     /gene="ychE"
                     /locus_tag="OQM52_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1565647.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1468..4143
                     /gene="adhE"
                     /locus_tag="OQM52_004017"
                     /gene_synonym="adhC"
     CDS             1468..4143
                     /gene="adhE"
                     /locus_tag="OQM52_004017"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1565648.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1166 a         1015 c          958 g         1030 t
ORIGIN      
        1 caatcaaata gagattggct aagcatgtct atcttttctg attacagttc cagtagtgaa
       61 atgcacaata atttaacaat tgattattat ttagcacttt cctctaccaa aggcagtgga
      121 attactaata tcatttcaat tattcttcag caagcccagg attatgatgt ggcgaaaata
      181 acatgatggt gaggagaagc agttaaggaa atccttataa ctaactgtaa tttgagaaga
      241 atatttttac ctattccttt tgcttttgat aactgcgcgg gaacaaagta aaaataatga
      301 cgaagtaaac ggagcatggc agctccgttt catttgaaag gaattaatta agcaggccgg
      361 ggaaaatccc cttaatacca gtaacgataa attcaatccc caatgccatc agcaatagcc
      421 ccataatacg cgtaatcacg ttgatgccgg tctggcgtaa aacccgtacc agccacggtg
      481 ccatgcggaa caatccccag caacataaag cgaacaatgc aatagccaca aagaaaccaa
      541 acagatagct aatgctgtga taacgcgtac cccagacgat ggtagaactg atcgcccctg
      601 gccccgccat caacggcaac gccaatggca ccacaccaat actttcacgt accgcggttt
      661 ctgatttttc ttgcttgttc tgtttatcct cgccaagctt gccgctgatc atcgacatcg
      721 ctatcgtcac caccaggatg cccccggcga tacggaacga atcaattgat ataccaaaaa
      781 gttgtagaat cgtgtcgccg agaaaaagcg agatccacaa gataatggcc acagacaggt
      841 tggctgtaag gttagttttg tttcgcgctg ccgctgtctg ataactggtc atgctgataa
      901 agacgggaat aatccctacc gggttgacca gcgcaaataa cccgatgaaa aatttgaagt
      961 aaacgggaaa atcaaaaagg gtctgaatca cggttcgctc cgaagcaaaa gccggataat
     1021 gttagccata aataaggttg aaaagacgcg ctgacaatac gccttttgac agcatttttc
     1081 acctcctaac tacttaaaat tgctatcatt cgttattgtt atctagttgt gcaaaacatg
     1141 ctaatgtagc caccaaatca tactacaatt tattaactgt tagctataat ggcgaaaagc
     1201 gatgctgaaa ggtgtcagct ttgcaaaaat ttgatttgga tcacgtaatc agtacccaga
     1261 agtgagtaat cttgcttacg ccacctggaa gtgacgcatt agagataata actctaatgt
     1321 ttaaactctt ttagtaaatc acagtgagtg tgagcgcgag taagcttttg attttcatcg
     1381 gttaagcaaa tcatcaccgc actgactata ctctcgtatt cgagcagatg atttactaaa
     1441 aaagtttaac attatcagga gagcattatg gctgttacta atgtcgctga acttaacgca
     1501 ctcgtagagc gtgtaaaaaa agcccagcgt gaatatgcca gtttcactca agagcaagta
     1561 gacaaaatct tccgcgccgc cgctctggct gctgcagatg ctcgaatccc actcgcgaaa
     1621 atggccgttg ccgaatccgg catgggtatc gtcgaagata aagtgatcaa aaaccacttt
     1681 gcttctgaat atatctacaa cgcctataaa gatgaaaaaa cctgtggtgt tctgtctgaa
     1741 gacgacactt ttggtaccat cactatcgct gaaccaatcg gtattatttg cggtatcgtt
     1801 ccgaccacta acccgacttc aactgctatc ttcaaatcgc tgatcagcct gaagacccgt
     1861 aacgccatta tcttctcccc gcacccgcgt gcaaaagatg caaccaacaa agcggctgat
     1921 atcgttctgc aggctgctat cgctgccggt gctccgaaag atctgatcgg ctggatcgat
     1981 caaccttctg ttgaactgtc taacgcactg atgcaccacc cagacatcaa cctgatcctc
     2041 gcgactggtg gtccgggcat ggttaaagcc gcatacagct ccggtaaacc agctatcggt
     2101 gtaggcgcgg gtaacactcc agttgttatt gatgaaactg ctgatatcaa acgtgcagtt
     2161 gcatctgtac tgatgtccaa aaccttcgac aacggcgtaa tctgtgcttc tgaacagtct
     2221 gttgttgttg ttgactctgt ttatgacgca gtacgtgaac gtttcgcaac ccacggcggc
     2281 tatctgttgc agggtaaaga gctgaaagct gttcaggatg ttatcctgaa aaatggtgcg
     2341 ctgaacgcgg ctatcgttgg tcagccagcc tataaaattg ctgaactggc aggcttctct
     2401 gtaccagaaa acaccaagat tctgatcggt gaagtgaccg ttgttgatga aagcgaaccg
     2461 ttcgcacatg aaaaactgtc cccgactctg gcaatgtacc gcgctaaaga tttcgaagac
     2521 gcggtagaaa aagcagagaa actggttgct atgggcggta tcggtcatac ctcttgcctg
     2581 tacactgacc aggataacca accggctcgc gtttcttact tcggtcagaa aatgaaaaca
     2641 gctcgtatcc tgattaacac cccggcttct cagggtggta tcggtgacct gtataacttc
     2701 aaactcgcac cttccctgac tctgggttgt ggttcctggg gtggtaactc catctctgaa
     2761 aacgttggtc cgaaacacct gatcaacaag aaaaccgttg ctaagcgagc tgaaaacatg
     2821 ttgtggcaca aacttccgaa atctatctac ttccgccgtg gctccctgcc aatcgcgctg
     2881 gatgaagtga ttactgatgg ccacaaacgt gcgctcatcg tgactgaccg cttcctgttc
     2941 aacaatggtt atgctgatca gatcacttcc gtactgaaag cagcaggcgt tgaaactgaa
     3001 gtcttcttcg aagtagaagc ggacccgacc ctgagcatcg ttcgtaaagg tgcagaactg
     3061 gcaaactcct tcaaaccaga cgtgattatc gcgctgggtg gtggttcccc gatggacgcc
     3121 gcgaagatca tgtgggttat gtacgaacat ccggaaactc acttcgaaga gctggcgctg
     3181 cgctttatgg atatccgtaa acgtatctac aagttcccga aaatgggcgt gaaagcgaaa
     3241 atgatcgctg tcaccaccac ttctggtaca ggttctgaag tcactccgtt tgcggttgta
     3301 actgacgacg ctactggtca gaaatacccg ctggcggact atgcgctgac cccggatatg
     3361 gcgattgtcg acgccaacct ggttatggac atgccgaagt ccctgtgtgc tttcggtggt
     3421 ctggacgcag taactcacgc catggaagct tatgtttctg tactggcatc tgagttctct
     3481 gatggtcagg ctctgcaggc actgaaactg ctgaaagaat atctgccagc gtcctaccac
     3541 gaagggtcta aaaatccggt agcgcgtgaa cgtgttcaca gtgcagcaac tatcgcgggt
     3601 atcgcatttg cgaacgcctt cctgggtgta tgtcactcaa tggcgcacaa actgggttcc
     3661 cagttccata ttccgcacgg tctggcaaac gccctgctga tttgtaacgt tattcgctac
     3721 aatgcgaacg acaacccgac caagcagact gcattcagcc agtatgaccg tccgcaggct
     3781 cgccgtcgtt atgctgaaat tgccgaccac ctgggtctga gcgcaccggg cgaccgtact
     3841 gctgctaaga tcgagaaact gctggcatgg ctggaaacgc tgaaagctga actgggtatt
     3901 ccgaaatcta tccgtgaagc tggcgttcag gaagcagact tcctggcgaa cgtggataaa
     3961 ctgtctgaag atgcattcga tgaccagtgc accggcgcta acccgcgtta cccgctgatc
     4021 tccgagctga aacagattct gctggatacc tactacggtc gtgattatgt agaagatgaa
     4081 actgcagcga aaaaagaagc cgctccggct aaagctgaga aaaaagcgaa aaaatccgct
     4141 taatcagtag cgctgtctgg caatataaa
//
LOCUS       DAJVQR010000193         4043 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVQR010000193 DAJVQR010000000
VERSION     DAJVQR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(163..693)
                     /gene="cybB"
                     /locus_tag="OQM52_004018"
     CDS             complement(163..693)
                     /gene="cybB"
                     /locus_tag="OQM52_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1565649.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            882..1882
                     /gene="gap"
                     /locus_tag="OQM52_004019"
                     /pseudo
     CDS             882..1882
                     /gene="gap"
                     /locus_tag="OQM52_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(1924..3363)
                     /gene="aldA"
                     /locus_tag="OQM52_004020"
     CDS             complement(1924..3363)
                     /gene="aldA"
                     /locus_tag="OQM52_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS1565650.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3560..>4043)
                     /locus_tag="OQM52_004021"
     CDS             complement(3560..>4043)
                     /locus_tag="OQM52_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1565651.1"
                     /translation="HQFWHIPHEKIWIEDQSANCGENARFSITLLNQAVERVHTAIVV
                     QDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERY
                     LSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR
                     "
BASE COUNT         1069 a         1017 c          934 g         1023 t
ORIGIN      
        1 gatcgggtga attgattaaa aataagccag atggacgtat gggcaaggat tatggtttta
       61 tttgtcatac aaataaatat aataggcgct tccgatgtag acccgtattc ttcgcctgta
      121 ccacgggtcg gttttagtac aggcgttttc tttaaatatc cttcaggaac gtttacgcgg
      181 catcatgcgt agaagtgtgt tgtccttcca gaaatagtgg tgtgccagtg cagccgcagc
      241 gtgcaacccg atgacaaaat atcccagatt cgccagcgtc tcatgccacg actttaagct
      301 atcaacccgt tcgaaattgg cctctgaagc gtaaggcatc gtcaaaccaa acgcaaacca
      361 cgggttgccc cggttataca tcatcaccaa accaatcact ggcagcgcaa taaacaggag
      421 ataaatcacc aaatgtccca aatgcgccag tcccgtcatc atcggtttcg gcttaggtat
      481 aatcggcggg gttgggtatt tcagtcttaa caaaagacga accaccatca gcacgagaat
      541 tgagatgcca caggaaacat gaatcatgtt gatgagcggc cgatcgctac gtgggaagaa
      601 accacgaaac tccattgcgc aatacgctgc gataaccagc aaaaagacca gccagtgaat
      661 gctgatttgt aaccttgagt atttattttc cataacattt cctgctttaa cataattttc
      721 cgttaacata acgggctttt ctcaaaattt cattaaatat tgttcacccg ttttcagtac
      781 gttacgtttc actgtaagaa tggagattgc ccatcccaca atcctggtct aagcctggaa
      841 aggatcaatt ttcatccgaa cgttcctgac aggagaaaac catgagtaaa gttggtatta
      901 acggttttgg tcgtatcggt cgactggtgt tgcgtcgttt acttgaagtc aaaagcaaca
      961 tagacgttgt cgctattaat gatcttactt ccccaaaaat tctcgcctat ctgctgaaac
     1021 atgattcaaa ctatggtccg ttcccctgga gcgttgattt tacggaagat tcacttatcg
     1081 ttgatgggaa aagtatcgcg gtttacgccg aaaaagaggc taaaaatatt ccgtggaaag
     1141 cgaaaggggc agaaatcatt gtcgaatgta ctggctttta tacctccgcc gataaatcgc
     1201 aggcgcatct tgatgctggt gcgaagaagg tgttgatttc cgctcctgcc ggtgaaatga
     1261 aaaccatcgt ttataacgtt aatgacgaca ctctggatgg caacgacacc attgtttccg
     1321 tggcgtcatg caccactaac tgccttgcgc cgatggccaa agccttgcat gacagtttcg
     1381 ggatagaagt cggcacgatg acgaccattc atgcctatac tggcacccag tcactggtgg
     1441 atggcccgcg tggtaaagat ttacgtgctt cacgcgcagc ggcagaaaat atcattcccc
     1501 acactacggg ggcggcaaaa gccattggtc tggtgatccc ggaactgagc ggcaaactga
     1561 aaggtcatgc gcaacgcgtg ccggtgaaaa caggttcggt cactgagctg gtgtccattc
     1621 tcggaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1681 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1741 cggttcagtg tttgatgcca cgcaaacgga aattaccgcc gtgggcgatt tacaactggt
     1801 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct
     1861 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     1921 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     1981 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2041 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2101 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2161 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2221 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2281 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2341 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2401 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gcagattgac
     2461 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2521 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2581 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2641 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2701 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     2761 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     2821 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     2881 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     2941 acgtttaaac agaagaatat tttctcccgg acgatcgctt tgaataatct cgccctcgta
     3001 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3061 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3121 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3181 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3241 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3301 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3361 catgggcgac tcttgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3421 aatcatcaga gagacggcat cgggaaagga caagtctgtg gagggcttca taaaaacaag
     3481 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3541 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3601 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3661 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3721 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     3781 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     3841 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     3901 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     3961 tgctaaagcg tgcgttttca ccgcagtttg ctgactggtc ttcaatccag attttttcat
     4021 gcggaatatg ccagaactga tga
//
LOCUS       DAJVQR010000194         4042 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVQR010000194 DAJVQR010000000
VERSION     DAJVQR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(886..1077)
                     /locus_tag="OQM52_004022"
     CDS             complement(886..1077)
                     /locus_tag="OQM52_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1565652.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OQM52_004023"
     CDS             1142..1465
                     /locus_tag="OQM52_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCS1565653.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OQM52_004024"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OQM52_004024"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OQM52_004025"
     CDS             2143..2673
                     /locus_tag="OQM52_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCS1565654.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OQM52_004026"
     CDS             2680..2913
                     /locus_tag="OQM52_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1565655.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OQM52_004027"
     CDS             complement(3459..3851)
                     /locus_tag="OQM52_004027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565656.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJVQR010000195         4038 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVQR010000195 DAJVQR010000000
VERSION     DAJVQR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..82)
                     /locus_tag="OQM52_004028"
     CDS             complement(<1..82)
                     /locus_tag="OQM52_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCS1565657.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQL"
     gene            complement(232..393)
                     /locus_tag="OQM52_004029"
     CDS             complement(232..393)
                     /locus_tag="OQM52_004029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565658.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(390..578)
                     /locus_tag="OQM52_004030"
     CDS             complement(390..578)
                     /locus_tag="OQM52_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1565659.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            838..1173
                     /gene="iraM"
                     /locus_tag="OQM52_004031"
     CDS             838..1173
                     /gene="iraM"
                     /locus_tag="OQM52_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1565660.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1454..1562)
                     /locus_tag="OQM52_004032"
                     /pseudo
     CDS             complement(1454..1562)
                     /locus_tag="OQM52_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2085..2161)
                     /locus_tag="OQM52_004033"
     tRNA            complement(2085..2161)
                     /locus_tag="OQM52_004033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2125..2127),aa:Arg,seq:tct)
     gene            complement(2263..2338)
                     /locus_tag="OQM52_004034"
     tRNA            complement(2263..2338)
                     /locus_tag="OQM52_004034"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2303..2305),aa:Ile,seq:cat)
     gene            complement(2483..3304)
                     /locus_tag="OQM52_004035"
     CDS             complement(2483..3304)
                     /locus_tag="OQM52_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1565661.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3319..3675)
                     /locus_tag="OQM52_004036"
     CDS             complement(3319..3675)
                     /locus_tag="OQM52_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1565662.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3688..>4038)
                     /locus_tag="OQM52_004037"
     CDS             complement(3688..>4038)
                     /locus_tag="OQM52_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1565663.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1135 a         1088 c          777 g         1038 t
ORIGIN      
        1 gcaactgcag gaaccagtag cccgttccca ccgctgacgt ggtgtatgac acacctgttg
       61 tgattttttc catctggtcc ataccccgtc tcccgttatc cggaagctga caacaataaa
      121 aaagccacca gttaactact gatggctctg ataactcatg caagcgtctc agacgaccca
      181 ctgacactac cggtgagttt aacgatacct tccatttgac tggctcactt tttatgatga
      241 tgccggtgca tttatctcca gcaccagact ttctatctca acgccatacg ttgcattttt
      301 ggtaatatcc gtcagcgtca gtgcatttag tcccactgcc agactgtctt ttatggcctg
      361 gaatgccggg ccagtacgat gacgtagtat cactccggct cagttgcacc actgaccacc
      421 acatcacctt ctgctgcaat cgcctgcatc agggtataag gggttatggc caccggacta
      481 ccaaacggct gccagccctc tttcagttta tgtgtcagct tttccgcaag atctgacggc
      541 ggcgccgccc tgacaacatc atagtgttta aatgccatgg ttctttccaa catctgaaaa
      601 ataattcttt aaaatacctg acatgtaata cagaaaaaac acaaaaccat accttaaata
      661 aaaacctgat tatcaagcag atatgcatgg ataaactaca agacgagata taaaccaccc
      721 tgcatttaaa taaacaataa acaacatcag aaaaataatt ctgttctatg gtttaattca
      781 aaaatatcat ttatactttt cagaacatca ccagcaaggc ataaacaagg aaactaaatg
      841 aagtggattg tgattgatac agttatccag ccatcatgcg gaatatcttt ttcagtcata
      901 tggagtaaaa taaaattaat aatctggtat caatcggatg ctttcttacc tcctgaaagt
      961 atatttacac tgactcacac aggcatcatg ctcaataaca aagtgctacc tgtaaccatt
     1021 tacaacgtag taccattcaa taaaacattc tggaatttaa tcaaaaacag ccaggaatgc
     1081 cctacaaata cagataacgt attgaatgaa tgctttaata accgttgcac tctgcaaata
     1141 tgcccttatg gactaaaaca acaaagtcca taaggagttt actcacatct gacaaaatca
     1201 atataaacag cccctcggga gaggggctgg agagtggcgc tatgtgccat tccatggtgc
     1261 cgggtgcctc ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta
     1321 ctcgctgatt gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc
     1381 ccgcaaacca gacaacagtc aaccgcctga attgtgaagt atttaaaaat ttctcccgct
     1441 aactgatacc cggctaacag tctggcgttt tctttttcag caacgggaaa gcaacaacca
     1501 ccacacccgc caccagcaca ccgtcagccg gcactgacat tatccggctg ctgcaatgcc
     1561 attaacaaaa acagtaagca atcacttttt accgtaacag gtgataatcc agatatgtat
     1621 ctaccccaga tgagtaatcc gaagttcatc cataccacag gtcctggcta ttctgttgta
     1681 ctcctgaaca agagcaaata attctgaatt agcaaccatg aactcatcgc aaaccctctg
     1741 tatagcatca ctattcagaa taataacgtc tcttcccgaa agacgatcag gagtacagaa
     1801 caaaactgtc aaacggctga aggcctttgc tcgtgctgca ttgactatat caatacgctg
     1861 cctaaggatg aaacaccccg acgcctcatc aatattcact ctacccacac catatgaatg
     1921 ataaatactt aatgctgaaa aaaccattag accgtataac aaatactcaa tcaatactta
     1981 acagaacttt tatttttgac aaatatataa tattttcaac aatatcctga gccaggtata
     2041 tttcagtata aggctctgcc ggaaggaatc tggaagaatg aatatggcgc gctgtactgg
     2101 attcgaacca gtgaccgatt gcttagaagg caattgctct gtccggctga gctaacaacg
     2161 ctgaataccg ataatggacc gccatcgggg acccgccccc cgcaccaaca accctgttat
     2221 cgtgtcgtct gctcttcctg ataagctaat ggcggtttgt gatggtggcc cttgctggat
     2281 ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccgg
     2341 gagcagaata ataacggtcc gtaattaatt tcgcaataaa aaaaaccgct cagcggcggg
     2401 ttgtagaaac tcttctaacg ccaggcataa aacgcccatc attatgacga atttaccaca
     2461 gattccggaa aaatcaacct tgttacctag ttaccttttt taactgccgc tcagcccatg
     2521 cttcttcaat atcaaaccgg gtcaccagcg catcataaaa tttcttaact gttttttccc
     2581 atgacgcgcg tgttatctgg tttgtcacct cgcatatagc attaaatgcc tccgttgatg
     2641 gtagtctttc atagccacga ccaccacaac gctggcagtc tctgataaca ggcataccac
     2701 gttttaccga ctcttcacga tgaatggcga caccacgccc acggcaatcc ttacaggcgg
     2761 tggaaacctc accctttccg ccacactccg gacaggcaac ttttaccacc tccctgactt
     2821 ttttccattc ttcccagtaa aacggataca cacctttcgt acactttgcc cataccggcg
     2881 gcttaccatc cggatactgg atcttgtttg taaaaactat gctttcaata aatttttccc
     2941 catagcaaca agggcactgc tttttactcg ctgcgctgcg ggcataatcc tcaaaagcgt
     3001 acgaagccat aatgcgcatc actaccggtt ttatttctgc cggaagtttt ctcaacgccg
     3061 ccacacgatc gcaccgactg agtgcataat ctgccagtaa ttctgttgcc cgcgccctgt
     3121 cattcatact gatgcccatt ttccccagga acgcagaaaa acccatctca gcccgattct
     3181 gtgtcatgcc ctgcgcggcc atcacatcag tgatactcag cgcatctttt gacgttgagg
     3241 ccgatgcatc ggtcaggcca ggggattttg gggagtagta tttcggtaaa tcttccagtt
     3301 tcattttttg acctgctctt aatgcattat ggggtaaatc ttcaccccca gacgtccacc
     3361 agatactggc tgaccacgaa cgatattgat ttcatcaaac tgctcatcgt ccattaacac
     3421 tcccgcatgc gtcagcgcat ccagcggtgc tttcaggata ttgtccaggt cgcgacgacg
     3481 cttatccggt ggctctgcaa tcacctttat cgccagcctt ccggacaggc ttaatttcag
     3541 ccgctgctgg cgaacaataa gcgccacagc ccggcgataa cgctttccct cctccgagat
     3601 aaaatatgtg ctgccacggc gtcgccagta agtgttcacc gtcggcgggt aaggtaaaac
     3661 caaatctatg agcatcagtc acctctttta cccaagcacg ccagttgcaa aggcgtgatc
     3721 aagaaaacga aaaattaaat caacctgaga accatgcttt tcttcgaacg ccagaggatc
     3781 cgcatgaagc tcgttgtgat gctcccgaca cagcggtagc gtgaaaatat cgtgagattt
     3841 ggtccccatt ccgccctgac catgaccaat caggtgatgg ggatcgtcgg ctggcttacc
     3901 acaacacgca cacggctgtg tcttcaccca gcgtttgtat ttctcgttaa cccagcggcg
     3961 acgtttaggt cgtttcatga aagattccgg agactcagga tcaacggcaa tgctgaccac
     4021 cgtcttttcc tgttgtgg
//
LOCUS       DAJVQR010000196         3887 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVQR010000196 DAJVQR010000000
VERSION     DAJVQR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(31..2064)
                     /gene="metG"
                     /locus_tag="OQM52_004038"
     CDS             complement(31..2064)
                     /gene="metG"
                     /locus_tag="OQM52_004038"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1565664.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2196..3305
                     /gene="apbC"
                     /locus_tag="OQM52_004039"
     CDS             2196..3305
                     /gene="apbC"
                     /locus_tag="OQM52_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1565665.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3567..3848
                     /locus_tag="OQM52_004040"
     CDS             3567..3848
                     /locus_tag="OQM52_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1565666.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          939 a          970 c         1014 g          964 t
ORIGIN      
        1 gcgctgtcga tgcagcgcct tgaaggggga ttatttcacc tgatgacccg gtttagcacc
       61 ggcatccggg cttagcagga aaatatcttt cccgccagga ccggcagcca tcaccatgcc
      121 ttcagagata ccgaagcgca ttttacgtgg tgccaggtta gccaccataa tggtgtgacg
      181 accaatcagt gcctgcggat ccgggtaagc agaacgaata ccggagaaga cattgcgttt
      241 ttcaccgccg agatccagcg tcaggcgcag cagtttgtca gaaccttcaa caaactctgc
      301 gttttcgatc agcgccacgc gcaggtcaac tttggcgaag tcgtcaaagg tgatggtttc
      361 ctgaatcgga tcatctgcca gtgggccagt aactggcgcg gtagtggctt tcacttcttc
      421 tttagaggct tccaccagag cttcaacctg tttcatatcg atgcggttat acagcgcctt
      481 gaacggattc actttgtggc ccagcagcgg ttgctggata ccatcccagg tcagttccgt
      541 attgaggaag gcttctgcac gctcggtcag tttcggcagt acaggcttca ggtaagtcat
      601 cagtacgcgg aacaggttga tgcccattga gcaaatcgcc tgcaggtcag catcgcgacc
      661 ttcctgtttt gcgactaccc acggagcctg ctcgtcgaca tagcggttag ccaggtcagc
      721 cagtgccatg atttcgcgca ctgctttacc aaattcacgg ctttcccacg cttcaccaat
      781 cacttcagcg gcatcggtaa aggttttgta cagctcaggg tcagccagct cgcttgccag
      841 cacaccgtca aaacgcttgt tgataaagcc cgcattacgg gaggccaggt taaccacttt
      901 gttcacgata tcggcattca cacgctgaac gaaatcttcc aggttgagat cgatatcatc
      961 aatgcgcgaa gagagtttcg cagtgtagta gtaacgcagg ctgtcagcgt caaaatgatt
     1021 cagccaggtg ctggctttaa taaaggtgcc gcgagacttg gacatcttcg cgccgttcac
     1081 cgtcacatag ccatgaacaa acaggttggt cggcttgcgg aagttgctgc cttccagcat
     1141 ggcaggccag aacaggctgt ggaagtaaac gatatcttta ccgatgaagt ggtacagctc
     1201 ggcggtggag tctttcttcc agtattcatc gaagcttacg ctgtcgccgc gcttgtcgca
     1261 cagattcttg aaagaaccca tgtagccaat cggtgcgtcc agccagacgt agaaatattt
     1321 gcccggtgcg ttcggaattt caaaaccaaa gtatggcgcg tcgcgggaga tatcccactg
     1381 ttgcaggccg gattcaaacc actcctgcat tttgtttgcc acctgctcct gcaacgcacc
     1441 gctgcgggtc catgcctgca gcatttcgct gaaagagggc agatcaaaga agaagtgttc
     1501 agaatcacgc atcactggtg tagcaccaga aaccaccgat ttcggctcga tcaattcagt
     1561 cgggctgtag gtcgcgccgc agacttcgca gttatcgccg tattgatccg gggatttaca
     1621 tttcgggcag gtgcctttca caaaacggtc cggcaggaac atgccttttt ccggatcgta
     1681 cagctgagag atggtgcggt ttttaataaa accgttttct ttcaggcgag agtagataag
     1741 ttctgacaac tggcggttct cttcgctgtg cgtcgagtga tagttgtcat agctgatgtt
     1801 aaagcctgcg aaatcagtct gatgctcctg actcatttcg ccaatcatct gctccggggt
     1861 gataccgagc tgctgagctt tcagcatgat cggtgtaccg tgggcatcgt cggcgcagat
     1921 gaagttgacc tcgtggccgc gcattcgctg gtaacggacc cagacatcag cctggatgtg
     1981 ctccagcata tggccgaggt ggattgagcc gttagcgtac ggcagtgcgc acgtcaccag
     2041 aattttcttc gcgacttgag tcatagtagg cattacttct taatagtgaa aaggggttag
     2101 atagtaccaa atgggaaaat gttaagtaag tacttctcta tattcttcag gtaaactatc
     2161 agctgatggt ttatctatac aaaaaaggag tcgggatgaa cgaacaatcc caggccaaat
     2221 caccagaagc cttgcgcgca atggtcgccg ggacactggc caattttcag cacccaaccc
     2281 tgaagcataa cctcaccacg ctgaaagcgt tgcaccatgt cgcctggatg gacgacacgt
     2341 tgcatgtgga actggttatg ccgtttgtct ggcatagcgc gttcgaagag ttaaaagagc
     2401 aatgtagcgc cgaactgctg cgcatcaccg gggcgaaagc tatcgactgg aaactgtcgc
     2461 ataacatagc cactctgaaa cgcgtgaaga atcagccggg aattaacggt gtgaagaaca
     2521 ttatcgcggt gagttccggt aaaggcggcg tgggtaaatc gtccacggcg gtaaacctgg
     2581 cgctggcact ggcggcggaa ggggcgaaag tcggcattct ggacgccgat atctacggcc
     2641 catcaattcc caccatgctg ggcgcagaaa atcagcgtcc aacttcgccg gacggtaccc
     2701 acatggcacc gattatgtct catggcctgg caaccaattc aattggttat ctggtcactg
     2761 acgacaatgc gatggtgtgg cgtggaccga tggcgggcaa ggcgttaatg cagatgttgc
     2821 aggaaacctt gtggccggat ctcgactatc tggtgcttga tatgccgcca ggcaccggtg
     2881 acattcaatt gacgctggcg caaaacattc cagtaactgg tgcagtcgtg gtcaccaccc
     2941 cgcaggacat cgcgttgatc gatgcgaaaa aaggcattgt gatgttcgag aaagtcgagg
     3001 ttccggtgct cggtattgtc gagaacatga gtgtgcatat ttgcagtaac tgtggtcacc
     3061 atgaaccgat tttcggtacc ggtggtgcag agaaactggc tgaaaaatac catacacagc
     3121 tgctggggca gatgccgtta cacatctcct tacgtgagga tctcgataaa ggcacgccaa
     3181 cggttatcag ccgtccggag agcgaattta ccgctatcta ccgccagctg gctgaccgcg
     3241 ttgcagctca gctctactgg cagggtgaag tcattcctgg cgagatttcc ttccgcgcgg
     3301 tctaacgcct attaaccatt ctggttattc ttcatacacc gcagagatgt tatattgatg
     3361 cggtgtattt gtttttgccg cattaatgaa cacttattta atactgcgaa tattctgcgg
     3421 accttcgtcc gaaactctac aaagtcgagt gatcgtacag gcaatgcttc attatcatcc
     3481 ttcgtgctgt gtagtggatg tacagctata gtttgttatg cactcattct atccaggcat
     3541 aacctttact acagggataa cgttcaatga ataagtattg gctgtcagga attatttttc
     3601 ttgcctatgg attggcttct cccgcagtct cttcagagac tgccacatta acgattaatg
     3661 gcaggatcag tcccccaaca tgcagtatgg ctatggttaa tagtcaacca caacagcatt
     3721 gtggtcagtt aacgtataat gttgacaccc gtcatcaggt ttcttcgccc gtcaaaggtg
     3781 ttacgacaga agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct
     3841 acgattaatt tggcaacctg attttaattg catttagaaa gacgaaa
//
LOCUS       DAJVQR010000197         3829 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVQR010000197 DAJVQR010000000
VERSION     DAJVQR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..224
                     /locus_tag="OQM52_004041"
     CDS             <1..224
                     /locus_tag="OQM52_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1565667.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OQM52_004042"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OQM52_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1565668.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OQM52_004043"
     CDS             518..1108
                     /locus_tag="OQM52_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1565669.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVPQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OQM52_004044"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OQM52_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCS1565670.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OQM52_004045"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OQM52_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCS1565671.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OQM52_004046"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OQM52_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCS1565672.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OQM52_004047"
     CDS             3457..3648
                     /locus_tag="OQM52_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565673.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1048 a          930 c          957 g          894 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgc ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctccatat gaacggaacc
     3781 gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggtt
//
LOCUS       DAJVQR010000198         3816 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVQR010000198 DAJVQR010000000
VERSION     DAJVQR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..183)
                     /locus_tag="OQM52_004048"
     CDS             complement(<1..183)
                     /locus_tag="OQM52_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCS1565674.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQI"
     gene            complement(134..898)
                     /locus_tag="OQM52_004049"
     CDS             complement(134..898)
                     /locus_tag="OQM52_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565675.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1093..1605
                     /locus_tag="OQM52_004050"
     CDS             1093..1605
                     /locus_tag="OQM52_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS1565676.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            complement(1694..2164)
                     /locus_tag="OQM52_004051"
     CDS             complement(1694..2164)
                     /locus_tag="OQM52_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1565677.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(2161..2694)
                     /locus_tag="OQM52_004052"
     CDS             complement(2161..2694)
                     /locus_tag="OQM52_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1565678.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(2760..3389)
                     /locus_tag="OQM52_004053"
     CDS             complement(2760..3389)
                     /locus_tag="OQM52_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565679.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(3393..3608)
                     /locus_tag="OQM52_004054"
     CDS             complement(3393..3608)
                     /locus_tag="OQM52_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1565680.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          938 a          988 c          843 g         1047 t
ORIGIN      
        1 gatctgacgg gcacaacaaa aacgcagggt gtatttattc gacagaaata tggcaatgcc
       61 agcggcatcc catgattttg acgatgaccg accaccataa agcactttgt tacgtgcccg
      121 catagtccag aaactacgta aagccggatt cagcgtcggt ttggatgtca gagtagaagt
      181 cattgaggtc acgctctcca ttgccatcat caatacctgc atcacggcga agacgatcag
      241 cctccagaga caccttatca gtggcggcct tgcggtaggc tgtatcagca aatatttttc
      301 ctaccgtcgc aagcgtgcca acgatggact caatacgaac tgtattgcgc atcattgctt
      361 tctcggcggc gctgatattt tcaatcagca tcttcctttc ctggtcccca ttagcatctt
      421 ccagcgacac caaccaccga ccaatattct ctgcagcgac aaggttgtta gcacgaaggc
      481 gaaataattc gtcctcgagc gtcaacgctt tagcgtcctc tatcacctca tctttgagca
      541 gaaggcgacg ggcataacca ccgtgtttta atgcctgctg gttaccgggt tggaatgggt
      601 tagtcggggg atcggtacgc attccgcgta tcggtttcgt atccggtgta gattctgttt
      661 ttagttgcgt acttttttgt gtaaggccag taatggcagg ctttctgctg gtacgcactt
      721 tccttttttg cgtaccattt ttgcaaacct gcgtaccgcc actgcgtgcc caatcaagct
      781 ttttggccct cttcctgata gccccttctg taacgccgta tttctcgcct atatcacgga
      841 ggctaaggac tccggcccgg tatgccgatt cgatggcctc ccagtccggt tttgccatga
      901 ttatgcttcc tgtgatttac tgttatcgct gcctgtcaca ataggttgct ttaattcttc
      961 ctcatcactc actcctgcga cgctccgtgg cagggccccc cacaggagta ccttgtaaat
     1021 gtaaggtgtt taagcctaaa ttaagtcgta tcacggttaa atagcaaaga aaaattcaca
     1081 gaaggaaaga cattgatgga gtggataaat gctttgatga actattttgt acttcatcca
     1141 tatcgcctat gggggcttct ttttataata gcgttcacaa aatccacttt actcatttcg
     1201 gcgatattgc caccagcatc aattatgatg gccactgtaa ttacagtcag caagacgact
     1261 ctttcaccat gggaagctgg aatcacggtc atgagtggcg catggtgcgg tactatcgtt
     1321 aattaccatc ttggtataat aattgggcat atccctcaac ttgcatgtat aatatcgagc
     1381 cgttccaata caatagaacg ggttcgcctt cagttgcaaa ataactctgt gtcaatactt
     1441 ttcacttcgc gctttatcgc cgtgctgcgt tacattgctc ctttggtggc aggaatgttg
     1501 caacttcatc cagtgaaagt gtatacagtg agtctgattt cagctgctag ctggtcagca
     1561 ctttatgttg gtagttttag cttcgtgttg ccttttttta gctaaactgc ccataaaaag
     1621 ctcttgcgaa tccagcttcc cccattcctc tgacaacaga atctgatttg aaggataatg
     1681 agtaaaatta ccgctacccc ccaaggcatt ggatcctgat gtactcctga agcattctca
     1741 atgctgtctg gtcgctgatg attccgtccc ggataccgag aacgtttcgt ccagcaactg
     1801 gagagagttc gacggtggca tcattgccca tgccggaggc gctggaggtt tcggctgagg
     1861 atggcacagg gcattttcct ttgacgagca cccgaccacc attatcaagc ttgcgccgaa
     1921 gagcatcatt ttcagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat
     1981 cagcatcacg ctggcgctgc tgcatgtcag taatggttgc gttcgccagc ttcagttctc
     2041 cggcgttttt gtcgcgctgc tctttgtagg taatggcgtt atcacggtaa tgattaacag
     2101 cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg gtgactctgc
     2161 tcattgatct atcccccaac aggctaatgc gctttcctgg tcacgacgaa taacctgtcc
     2221 atagcagtta tttgaacgta tgcggcaatc gcgcccaaca tcttttatcc accagcgaat
     2281 cgcctcgcat gcgcccttac gatcaccggc attcagccgc ttataaaacg tcgacgggaa
     2341 acacttaccg gggccaatgt tataggggca aaatgacgct atacccgctt tctggggttc
     2401 ggtcagaggt actttaatat tgcgctccac ccatgccagc gccttatcac gttcaatagc
     2461 gttaacctgg tcacattttt ccttcgacag cttcattccc ggtatgacag gcttaccatc
     2521 caccattgtg gcaccacgac agatggtcca tatgccagaa ccatcacggt atgccgttgt
     2581 gtgattaccc tctttttcgt tcagaaactg gtcaagtatc tggggagcag acgcgcctgc
     2641 ggcaatcagc gccagaacag cagccgacag gccgtatttg atttttgcgc tcatggatat
     2701 ttatcaggat ttatcggcaa caaataacga gccaacttat atacgccttt taagataagt
     2761 cagccccggc tggatccagt catcaggctc tttcttaaag ggggagtatt gaaatcacga
     2821 agaagagcct cccgcaccgc agcatccatg tctgcaccac tggcaagtgc ttcaatctct
     2881 gccgccacct gcaaataccc catgcaacgg ccaatgcgtt tcataactct ggtgttttcg
     2941 tctttcaggc atctgatagc gagattaata attttcaggc agtgatgatg cgatgcccgg
     3001 acaggttttc cgttctcata aacgacgaac tcaccctctt cacaggaaac cctgaaaggc
     3061 cgatggggtt cctggtcttt atccggtgca gacgcaataa aatcactttt atgctcttcc
     3121 tctccggcac tgttaaccgt aatgacgtac tcaggccgca ggtaagccgc ctctttttct
     3181 gacaatgcgg ccggtaaaaa cttttcctgc aaaaacttca taaaagtctc gtgagcgact
     3241 ttgtggcagt aatcgtaaat cgccgcataa tgctcatcgc ggcgtttgtt ttcatcttca
     3301 gaactcacca acgccgacaa ttttttatta agctcagtga tttcattttc caggtgattg
     3361 aatcgctgat tcatttcttc atgcttcatt atttactctc cccgtgcagc cttacgcttg
     3421 tcttctctta ttttgaaata cagattcgtc agataagtca gaagccccag aagcagactt
     3481 cccagcacac caatcgcagc ccactgtgat ggcctgacct gatccaacca ctgcaaaaac
     3541 cagtagccgg cactgccggc ggaggtgccg taggcaatgc ccgttgaaat tttgtccatg
     3601 gatttcatag cctcacctcc gcaaataacg gatggcgtag ttttacactg agaaatgaaa
     3661 gggatttgaa aagaaaaaaa cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca
     3721 ctctatccaa caaaccaccc acagttaatc ggaataaaag cagagtgctt atgaatgatc
     3781 gcacgcccga aggttagtat ttctgcacag caattt
//
LOCUS       DAJVQR010000199         3806 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVQR010000199 DAJVQR010000000
VERSION     DAJVQR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            437..2089
                     /locus_tag="OQM52_004055"
     CDS             437..2089
                     /locus_tag="OQM52_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1565681.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OQM52_004056"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OQM52_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OQM52_004057"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OQM52_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OQM52_004058"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OQM52_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1051 a          879 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cccccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJVQR010000200         3772 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVQR010000200 DAJVQR010000000
VERSION     DAJVQR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            87..755
                     /locus_tag="OQM52_004059"
     CDS             87..755
                     /locus_tag="OQM52_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565682.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(998..1693)
                     /locus_tag="OQM52_004060"
     CDS             complement(998..1693)
                     /locus_tag="OQM52_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS1565683.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1686..3113)
                     /locus_tag="OQM52_004061"
     CDS             complement(1686..3113)
                     /locus_tag="OQM52_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS1565684.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3124..>3772)
                     /locus_tag="OQM52_004062"
     CDS             complement(3124..>3772)
                     /locus_tag="OQM52_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1565685.1"
                     /translation="LLEKLGCRVNFPEKQGCCGQPAINSGYIKEAIPGMKNLIAALED
                     NDDPIISPAGSCTYAIKSYPTYLADEPEWASRAEKVAARMQDLTSFIVNKLGVVDVGA
                     SLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGLELLTFAEQDTCCGFGGTFSVKMAE
                     ISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISGRLQREGQKVKVMHIAEVLMSR"
BASE COUNT          984 a          988 c          802 g          998 t
ORIGIN      
        1 tttttgctga tatttaaccc gaacattcct gatacagact attaatacaa cattatctgc
       61 gacagatttt attttctgga tatattatga gtgaacaaat caaacaggat atcgatctga
      121 ttgagatttt atttcatctg aagaaaaaga ttcgtgttat cctttttatt atggctatct
      181 gtatggcaat ggtgctgttg tttctgtata tcaataaaga caatacaaaa gtgatttaca
      241 gccttaaaat aaaccagaca acgccaggta tacttgttag ctgtgatagc aataataatt
      301 ttgcctgtca gaccacaatg actgaagatg ttattcagcg aattactaca ttttttcaga
      361 ccagcccaga tatcaagaac agagaaataa ggctggaatg gtcaggagat aagagagatt
      421 taccaactgc tgaagaggaa atatctcgcg tgcaggcgtc tattatcaaa tggtatgcgt
      481 cagaatacca taatggcagg caagttctcg atgagataca aacgccttca gcaattaaca
      541 gtgagcttta tacaaaaatg atatacctga ccaggaactg gtcattgtat ccgaacggtg
      601 atggctgtgt aactataagc tcaccagaaa taaaaaataa ataccctgct gccatttgcc
      661 tggctctggg attttttcta agcattgtaa tttctgtaat gttttgcctt gtcaaaaaaa
      721 tggtagatga ataccaacaa aactctgggc agtaataaac agcattctga tagagagtgc
      781 ctgtataaag ctgaagtgac aatgaccggg aagggaaccc gttttcagga atggatgcct
      841 gtctttttca ctgtaaatat cctggcatta ctaatgcctg gatatttaca gaagagcagc
      901 ataaccatga cggaacgggg ttaagtcagg ttgtccttaa cgctattttc aacctcgtat
      961 gccttcttca ggtttatgtt cagatctcat atctctctca gcaatcctca ataatcagat
     1021 acaccgcttt caccggacca tgaaccccga cgactttgat aagctcaata tccgccgttg
     1081 agctgggacc actgatgatg ttaatacatg acggcattcg ttcaccggcc tgcgctttct
     1141 gatgcaattt ttctgcgagt tgcgctacac gcggcaggat tgtgctttta cgcaggataa
     1201 aaagagaaga ttccgggagc aggctcaatg aacgcccgcg ctcggcggcg gaaaaaagaa
     1261 ccacgcctcc cgattcggtt aaaccatatt cagcatacac gacacccact ttagcctgct
     1321 ctgcctgcga gatattctcg gcacctttcg ccggatccca aacaacggca ttgcattcct
     1381 gctgcaaacg ttcactaatc cccaattcct ccagcctcgt gtcaccgcta atcacgaccg
     1441 actgatctcc cagctcttta cacagacgta ttgcagcttc tgccgccttc gcctcgctgg
     1501 tcagctcaca gcgcgtcaac ataacatcgc tggcaaactg aataaacgcg tcacagcgct
     1561 gctgttggtt aagttgggta agccgctcgt tagcatagtt gttaagcggc gcatcttctg
     1621 cttgcggttc aagtcgcagc gggcgaccca gtgcctgagc aacgttattc aaaaattcgc
     1681 tccgattatc cattcttttt ctcctgcgcc tgatgtttct taaaccaact acggaaactc
     1741 tctccgtcag cttcaggaag atcgcgtgct tccatccagt cgctaatcgc gccaaatttg
     1801 agtggtgttt tgccgccatt gataaaccag cttgccgcat gagcgccggc catcatcccg
     1861 actttccaca atcctggatg actattggca taagcgaaca tttttatcgc ccgttgctct
     1921 gcttttgcgg tgatcccttt ttcagccatc acccgacgat gacgcaaaat cagttttgac
     1981 agcggaatac gcaccggaca cacgctgtca caagcagtgc ataaagagca ggcgtagggt
     2041 aaatctttaa aatctttata gccgccaagt agcggagaaa tcaccgcacc aattggccct
     2101 ggataaatag agccatatcc atgaccgcca atatggcgat atgccggaca agtattcata
     2161 caagccccgc agcgaataca gcgcagcaca tcccgaaatt cagaggccag cacctcagaa
     2221 cgcccgttat cgacaataac cagatgaaac tcttcaggac catcaacgtg ccccgcttcg
     2281 cgcggccctg tcagccaggt gttgtatccc gtcaaacgtg caccaacggc actgcgcgcc
     2341 agcatggtga tcaatacatc tacctcggca aacgtggggg caatacgctc cattcccatc
     2401 actgcaatat gcgttttagg cagcgtggta cacattcgcg cattaccttc attggtcacc
     2461 aggcataccg aaccggtctc tgccaccgcg aaattacagc cggtaatacc tatttcagca
     2521 ctgaggaaat cttcacggat tttttgccgg atgaataagg tcatcgcttc aggcgtttcc
     2581 ggcccctcat agcccagacg ttcgtgtagc actcgacgga tctgatggcg atctttatga
     2641 attgccggga ccacaacatg agatggcgga tcttgatcca gctggagaat atattcaccc
     2701 agatcggttt caatcacctg aatgccagca tcctgcaaca catgattgac accaatctct
     2761 tcggtcacca tcgatttaga tttcaccacc ttccgggcat ttttgcgttg ggcaacctgt
     2821 aaaatgtagc gggtagcgtc ttctttggtt tttgcaaaat agacgtgacc gccgttttgc
     2881 gtcacttttt ctgagagctg gtacagataa gcgtcgagat tactcagaac atgatcacgt
     2941 atctgggcgg cccgatcgcg ccactcctcc cagtgcccca attcatcgac cattttttgc
     3001 cgatttgccc caatacgctg ctgcgcgctt gccaccgctt tgcgcatgat cggatcttca
     3061 atttgctgac ggatgcgtgt cttaaaatct gtattactgg ttttgatcga catctttata
     3121 tcctcagcgg ctcatcaaca cttcagcaat atgcatcact ttgactttct gcccttcccg
     3181 ttgtaatcgc ccactgatgt tcagcaggca actcacgtca gcaccaatta aatactcagg
     3241 gcggacttcc atcaggtgca caaccttttc tttcaccatc tcgccggata tttcggccat
     3301 tttgaccgag aacgtgccgc caaatccgca gcaggtatcc tgttcagcaa aggtcaacag
     3361 ctccagtcca cgcacatttt tcagcagcgt aagtggctcg tccttcactc ccagcttacg
     3421 ggccaggcta caagatgggt gatacacagc tctcccttgc aaactggcac ctacatcgac
     3481 tacccctaat ttattaacaa taaaagaggt gagatcctgc atacgcgcgg caaccttttc
     3541 ggcacgtgat gcccattcag gttcatccgc cagatacgtc gggtaacttt ttatggcata
     3601 ggtgcaagag ccagccggtg aaataatggg atcgtcgtta tcctccagtg cggcgatcag
     3661 atttttcatc cctggaatcg cttctttgat ataaccgcta ttgatcgcag gctgaccgca
     3721 gcatccctgt ttctccggga aatttacgcg acagccgagt ttttccagta gc
//
LOCUS       DAJVQR010000201         3739 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVQR010000201 DAJVQR010000000
VERSION     DAJVQR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            109..2106
                     /gene="betT"
                     /locus_tag="OQM52_004063"
     CDS             109..2106
                     /gene="betT"
                     /locus_tag="OQM52_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCS1565686.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2169..3446
                     /locus_tag="OQM52_004064"
     CDS             2169..3446
                     /locus_tag="OQM52_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCS1565687.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          905 a          852 c          941 g         1041 t
ORIGIN      
        1 attccaccat gcattacgat taacgccgca tagccagttg aactttgcta ctttgtgaga
       61 ggtagtacct tctatccagt gcgaatttaa ttaatggaat aaatgattat gagtgaaaat
      121 gatacaatcc caaagaagtc tacaagtcag attaacaaag cggtattctt tacatctgct
      181 ttgctaattt tccttcttgt cgcctttgcc gccgtattcc cggatgtcgc cgacaaaaat
      241 tttaaactac ttcagcaaca aatcttcacg aatgccagct ggttctacat ccttgctgtg
      301 gccctgattt tactgagtgt cacgttcctt ggactctcac gctacggtga tatcaagctg
      361 ggcccggacc atgcgcagcc tgatttcagc taccactcct ggtttgcgat gcttttttcg
      421 gcagggatgg ggatcggcct gatgttcttt ggcgttgccg aacctgtaat gcattatctt
      481 tcgccacccg ttggcactcc agaaaccgtt gcggcagcta aggaagcaat gcgtctgacc
      541 tttttccact ggggactgca cgcatgggca atttatgcca ttgtggcgct gattctggcg
      601 ttcttcagtt accgtcacgg tctgccttta actctgcgct ccgcactcta tcccattatt
      661 ggcgatcgca tatacggacc tgtaggacat gcggttgata ttttcgctgt tataggcacg
      721 gtctttggcg ttgcgacatc actgggttac ggtgttttgc aggtgaatgc cggtttgaac
      781 catcttttcg gggtgcccat caatgaaacg gtgcaggtaa ttctgatcgt ggtcatcacg
      841 gggttagcga cgatttcagt ggtgtccggt ctggataagg gaatacgtat cctgtctgaa
      901 ctcaatctgg gtctggcttt gttgctcctg gcgctggtcc tgtgtctggg accaaccgtg
      961 cttctgctga agtcatttgt ggaaaatacg ggcggttatc tttcggaact ggtgagtaaa
     1021 acgttcaacc tttacgcgta tgagcccaag tcgagcaact ggctgggggg ctggacatta
     1081 ctgtactggg gatggtggct ttcatggtcg ccgtttgtgg ggatgttcat cgcacgggtc
     1141 tcccgcgggc gaaccattcg cgagtttgtc accggcgtgc tgtttgttcc cgcgggtttt
     1201 acgctaatgt ggatgacggt gtttggtaac agcgcgatct atctcattat gaaccagggg
     1261 gccacagacc tcgccaatac tgttcagcag gatgtggcgc tggccctgtt taatttcctg
     1321 gagcatttcc cgttctcttc tgtgctgtca ttcattgcaa tggcgatggt catcgtcttc
     1381 tttgtaacgt ctgctgattc gggggcaatg gttgtggata ctctggcatc aggtggagtg
     1441 gcaaacacac ccgtctggca gcgaatattc tgggcctcgc tcatgggcat tgttgcaatt
     1501 gcgcttctcc ttgccggagg gctaagtgcg ctgcaaacgg tgacaatagc gagtgcattg
     1561 cccttctcag tgatcttact aatatctata tacggacttt taaaagcttt gcgccgggat
     1621 ttgaccaagc gtgaaagcct gagcatggcg acaattgctc ctacggctgc acgtaaccca
     1681 attccttggc agagaaggtt acgcaatatc gcgtatctgc cgaagcgatc tcttgtgaaa
     1741 cgttttatgg acgacgttat ccagcctgcc atgacgctgg ttcaggagga actgaacaag
     1801 caggggacga taagccacat tagtgatgca gtcgacgatc gtattcgtct tgaagtcgat
     1861 ttgggcaacg agctgaattt tatatatgaa gtgaggcttc gcgggtatat ctcaccgacc
     1921 ttcgcgctcg ccgcaatgga taatgatgag cagcagactg aacaacatcg atattatcgc
     1981 gctgaggttt atctcaaaga aggcggtcaa aattatgatg tgatgggctg gaaccaggaa
     2041 cagctgatta atgacatact ggaccagtac gaaaaacacc tgcacttcct gcacctggtt
     2101 cgttaatagc aacatgccgt cctgggggcg gcaattatta tcccggccgc aatatgaggg
     2161 aatgcagaat gatttcacgc tggaaatgga tgctgaagca gacaattaaa aaactatggt
     2221 tcagggcaac gttattcgca attgtcgcga taataacggc ccttttatca attcttttta
     2281 aatcaatgat acctgagtcg gtttccgtga aggttggtgc ggaagcagtc gataacattc
     2341 tgaacatact ggcatcgagt atgctggcag tgaccacatt ttcgctgagt atcatggtca
     2401 cagcctacgg ttcagccact actaatgtga ctcccagagc tacgcgttta gttgttgaag
     2461 acgtcaccac acaaaatgta ctggccacct tcatcggttc ttttctcttc agtctggtag
     2521 ggattattgc cctcaatatg ggagcttatg gagaaagggg gagagtcatt ttattcattg
     2581 tcacactggt tgtcattgcc ttaatcctca tcacattgct tcgctggata cagcatttga
     2641 cctctctggg gagggttggt gagacaacgg cacaagtaga acaggcggcc atcgaaacat
     2701 ttattgcgag agcaagaaat ccctgtctcg gtggatatcc atggcttgag aacaatgaac
     2761 agccgaaagg aacggttgca gtttatccga agaagattgg ctatgttgaa tatattgata
     2821 tggtgaaact tagcaagctg ctgaccaatg atccccgtca tgtatacctc gtggcgcagc
     2881 caggcagttt catacatccg tccatgccag ttttgtacct gagtcagggc caggagtcgt
     2941 caatcaacac cgatttactt gagacgatta ttgtctcgga tgcacgttca tttgctcagg
     3001 atcctcgatt ttgtcttagc gtcatggccg aaatagcctg ccgggccctt tcccctgcag
     3061 tgaacgatcc tggaaccgcc attgatgtca ttggcagagg tgttcgtata ctttccactt
     3121 acgcgcagaa taaatctgat gaaatagaag tgaaatatcc ttcagtacat gttgcaccac
     3181 ttcagaataa cgatctactg gaagactttt tctcacctgt cgcgcgcgat ggtgccagta
     3241 tgagggagat tcagataaga gtccttaaag gactgtcgat gctgagtaaa ggttggcctg
     3301 agatatttgc tgaagccgca cataccctgg catttgaaac attagagcat gcaactcgtg
     3361 ctgaccatat agattctgat agatatctaa taaaatcaac ttattataat ttatttagtg
     3421 gcgaatattc taataaaaaa acatagctgc agagcaagca attcggttgt tggcaagtca
     3481 agggcaaagc attcgcgtga gaatgggcgt atgctttgtg ccaaaagcaa acgttgctaa
     3541 ttctgcactg ttcagcgatg gcgcagtaga gacaggcact gccactgtcc agtcggcaaa
     3601 agcgaacact ttgccactgc ggggataaac tgattgaagc tctcgcgcaa aatcacttat
     3661 tcttttgcgt taataaaatg taggagatgg gtcattgatc ctacccacgt aatatggaca
     3721 caggcctaag cgaggttct
//
LOCUS       DAJVQR010000202         3553 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVQR010000202 DAJVQR010000000
VERSION     DAJVQR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(1..1956)
                     /gene="flhA"
                     /locus_tag="OQM52_004065"
                     /pseudo
     CDS             complement(1..1956)
                     /gene="flhA"
                     /locus_tag="OQM52_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(1949..3096)
                     /gene="flhB"
                     /locus_tag="OQM52_004066"
                     /pseudo
     CDS             complement(1949..3096)
                     /gene="flhB"
                     /locus_tag="OQM52_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          885 a         1029 c          862 g          777 t
ORIGIN      
        1 cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat agagcttcct
       61 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
      121 cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc
      181 caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcgcttaatt
      241 catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat
      301 cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg
      361 tgacgacgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca
      421 acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt
      481 taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct
      541 gttcttttag cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg
      601 cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccagccag cgccccggat
      661 aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca
      721 gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt
      781 tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg
      841 ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg
      901 tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt
      961 tttgttcgcg tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa
     1021 ataccaggtt cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca
     1081 acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg
     1141 tgctgacacg cgtaacgatg ccccccgcgg cggtagaaat caccagcgcc ggaatttgtg
     1201 ccaccagacc gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca
     1261 tgccagttgc agcacgccga ccagcaaccc gccgacaacg ttaatgacca tgatgaggat
     1321 cccggcaatg gcatcgccgc gaacaaactt acttgccccg tccattgagc cgtaaaaatc
     1381 ggcttcctga gtcacttcgg agcggcgttt tttcgcctca tcttcaccaa tcaatccagc
     1441 gttaaggtcg gcgtcaatcg ccatctgctt acccggcata ccatcgagaa caaagcgcgc
     1501 acccacttct gctatacgcc cggcaccttt ggtaatgacc ataaagttga tgatcacgag
     1561 aatgacaaac accacgatac cgatagcgaa attgccacca acgaggaagt gaccgaacgc
     1621 ttcgaccacc ttcccagccg ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt
     1681 tgaagccacg ttaagtgcca gacgcaacaa catagtaaac aacagaatgg tcggaaacgc
     1741 agcaaactca agcgtgcgct gggtaaacat cgccaccagc aacaccatga tcgacaaggc
     1801 aatattgaag gtaaacaaca ggtcgagtat gaatgcgggc agtggcagca ccatcatcga
     1861 caagatcaac aggatcaaaa tcggtccggc aaggatctgc cattatgtcg atttcaggtt
     1921 tgcgggcagg cgcagcatcg cggccagatt actcatgggt cggtttctcg ttaataaaat
     1981 ccagggctgc cggcaccgga agatgagtag gtgtacaggg cgctgtccac cagccagacg
     2041 ccagcgtttc agttgccaga cccaggccag cacttccgcc accgcggcgt acagttgacc
     2101 cgggatttgt tgaccaatct ccgcatgtcg atacagcgct cgcgccagcg gcggcgcttc
     2161 aagcgtcggg acgttatttt cagcgccaat ttcacgaatg cgcagcgcga ccagccctgc
     2221 acctttagcg accactttcg gtgcgctcat tttgttttcg tcatactgca acgctaccga
     2281 atagtgggtc gggttattga caatgacatc cgctttcggc acatcggcca tcatccgacg
     2341 ccgtgcggca gctcgctgca tctgacagat ccgtccttta acatgggggt cgccttcgct
     2401 ttgtttgaac tcatcacgaa tatcctgccg cgacattcgc agctttttca ggtggctgaa
     2461 gatttggaaa aagacgtcaa atcccaccat tggaatgaca ccaagcacca ccagcagtgc
     2521 gcatagccct accaaatcca tcgcattacc catggcggta atcggtgact cggccattaa
     2581 gcgcatcatc tgcggccagt gatgccagag aaaaaacccc gtcacgctgc caaccaggat
     2641 ggttttcaaa attgccttaa gcaactccgc gctagtctgt gccgagaata tccgtttaat
     2701 gcccggtagc gggttgagtt tggaaaactt cggctgcaag gatttgccgc taaataccag
     2761 cccccccagc ataaccggag aaatgagcgc caccagcacc acgccgctaa tcagcggtag
     2821 cagcgccagc atggcttctc tgatcagcag aataatttgc ccgaggatca gattcgggtc
     2881 tttgataata ctgtgatcaa aatgcagccc agcggagagc atgcccgaca atcgacgggc
     2941 cagcgacaca ccgccaaacc agataacact aacgcccacc agcaaaatca gcagtgaggt
     3001 cagttcacgg gaacgcggga tttgcccctc ttcccgcgct ttttctagtc ggtgaggtgt
     3061 gggggcttct gttttgtcgt cgctctcgtc agacacgtcg ccaatcctgg aaagagtatt
     3121 aagcgtaaat gatgccagag cgcaaagcgt tcaatggttt aagtaagggg caaaacaggc
     3181 gggatttagg gcgtttatgc aaaaataggt tcagttgcag aacgttttgc gaaccatttt
     3241 ccagttctga tatttagcca tttagcctat agaatcgcac tccgcctctc tgttagtttg
     3301 ctaataatta aattattaca aaggtgatca aatggaatgg caacaattgc ttaactgcaa
     3361 ccgtcggaaa gataatctaa aaaaaaccaa catagcattt ttacatcccc ttgctagcgg
     3421 aagacaagag atcgagcggg attacgaccg attacttttt gctgcaccaa ctcgtcggct
     3481 tgcggataaa acacaagttt tcccgttaga tcaaaatgac agcatacgga cgaggctgac
     3541 tcattctcac gaa
//
LOCUS       DAJVQR010000203         3549 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVQR010000203 DAJVQR010000000
VERSION     DAJVQR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OQM52_004067"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OQM52_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1565688.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OQM52_004068"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OQM52_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1565689.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OQM52_004069"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OQM52_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1565690.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          820 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggcg
//
LOCUS       DAJVQR010000204         3543 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVQR010000204 DAJVQR010000000
VERSION     DAJVQR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..399)
                     /locus_tag="OQM52_004070"
     CDS             complement(<1..399)
                     /locus_tag="OQM52_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCS1565691.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAI"
     gene            complement(457..981)
                     /gene="sat2"
                     /locus_tag="OQM52_004071"
     CDS             complement(457..981)
                     /gene="sat2"
                     /locus_tag="OQM52_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1565692.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1076..1549)
                     /locus_tag="OQM52_004072"
     CDS             complement(1076..1549)
                     /locus_tag="OQM52_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1565693.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1881..2417
                     /locus_tag="OQM52_004073"
     CDS             1881..2417
                     /locus_tag="OQM52_004073"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS1565694.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2532..2858
                     /locus_tag="OQM52_004074"
     CDS             2532..2858
                     /locus_tag="OQM52_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1565695.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(3046..3285)
                     /locus_tag="OQM52_004075"
     CDS             complement(3046..3285)
                     /locus_tag="OQM52_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1565696.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          967 a          802 c          778 g          996 t
ORIGIN      
        1 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
       61 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
      121 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
      181 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
      241 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
      301 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
      361 tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaact cctgaattaa
      421 gccgcgccgc gaagcggtgt cggcttgaat gaattgttag gcgtcatcct gtgctcccga
      481 gaaccagtac cagtacatcg ctgtttcgtt cgagacttga ggtctagttt tatacgtgaa
      541 caggtcaatg ccgccgagag taaagccaca ttttgcgtac aaattgcagg caggtacatt
      601 gttcgtttgt gtctctaatc gtatgccaag gagctgtctg cttagtgccc actttttcgc
      661 aaattcgatg agactgtgcg cgactccttt gcctcggtgc gtgtgcgaca caacaatgtg
      721 ttcgatagag gctagatcgt tccatgttga gttgagttca atcttcccga caagctcttg
      781 gtcgatgaat gcgccatagc aagcagagtc ttcatcagag tcatcatccg agatgtaatc
      841 cttccggtag gggctcacac ttctggtaga tagttcaaag ccttggtcgg ataggtgcac
      901 atcgaacact tcacgaacaa tgaaatggtt ctcagcatcc aatgtttccg ccacctgctc
      961 agggatcacc gaaatcttca tatgacgcct aacgcctggc acagcggatc gcaaacctgg
     1021 cgcggctttt ggtacaaaag gcgtgacagg tttgcgaatc cgttgctgcc acttgttaac
     1081 ccttttgcca gatttggtaa ctataattta tgttagaggc gaagtcttgg gtaaaaactg
     1141 gcctaaaatt gctggggatt tcaggaaagt aaacatcacc ttccggctcg atgtctattg
     1201 tagatatatg tagtgtatct acttgatcga tcaggctttt gtatatctcc ccaccacctg
     1261 aaacaatgac atgatccgtt attttcttta ggttggttaa agcatcttta attgatggaa
     1321 agatcaatac gttctcattg tcagatgtaa aacttgaacg tgttacgacc gcatactttc
     1381 ggttgggtaa tgctcccatt gattcaaaag tcttgcgtcc aaccaacagc cattggttat
     1441 aggtaatagc tttaaacagg agctgttcac ctttggcact ccatggaata tcagggccat
     1501 tcccgataac tccattcttc gatatagcta ccattagtga tagtttcaca attcttcctc
     1561 agaggttaac attttattag taagcatgct cgttttgtca cccgctgatg cttaccgtta
     1621 attaatataa tcacttgtat taaatagact ttctttaaaa atacaagaca ctctgttatt
     1681 acaaatcgtg catgccgtct atcctgcttg cacgatgcac attatctcac ccaaagaact
     1741 cataacattt taatttattt agcattaaat taaaactcta ccggtaaaca agcatctcta
     1801 ggcgtaaaaa acaggcactt ttttaaatcc cagctagaat aggctgtata ggcagacagt
     1861 tgcaagacaa ggagcgtttt atgtctaaca gtccattttt aaattctata cgcacggata
     1921 tgcgacaaaa aggttatgcg ctgaaaactg aaaaaactta cctgcactgg attaagcgtt
     1981 ttattctgtt tcacaaaaaa cgtcatcctc agaccatggg cagtgaagag gtcaggctgt
     2041 ttttatccag cttagcaaac agcagacatg tagccataaa cacgcagaaa atcgctttaa
     2101 atgccctagc ttttttgtac aacaggtttt tacaacagcc gttgggcgat attgattata
     2161 tccctgcaag caagcctaga cggctaccct ctgttatctc tgcaaatgaa gtgcaacgca
     2221 ttttgcaggt tatggatact cgcaaccaag ttatttttac gctgctgtat ggtgcaggtt
     2281 tgcgcattaa tgaatgcttg cgtttgcggg ttaaagattt tgattttgat aatggctgca
     2341 tcactgtgca tgacggtaag ggtgggaaaa gcagaaacag cctactgccc acgcgcctaa
     2401 tcccagcaat aaaataactc attgagcaag cgcggcttat tcagcaagac gacaacttac
     2461 aaggcgtagg gccatcgctg ccttttgctt tagatcacaa atacccttct gcttatcgac
     2521 aagcggcgtg gatgtttgtc tttccctcca gcacgctctg caaccacccg tataacggca
     2581 aattatgccg ccatcatctg catgactccg ttgcgcgaaa ggcattgaag gcagccgtac
     2641 aaaaagcagg catcgttagc aagcgtgtca cttgtcatac atttcgtcac tcgtttgcta
     2701 cgcatctatt acaagcgggg cgtgatattc gcactgtgca agaactctta gggcataacg
     2761 atgttaagac cacgcaaatc tatacgcatg tgttgggtca gcattttgcc ggcaccacca
     2821 gtcctgcgga tggactgatg ctacttatca atcagtaaaa gtagataaat atctttatgt
     2881 agccctgagt agcccctgat actttttatg gtgaaaaaga taaacaatca tagaggggat
     2941 aaatatgagc cagttctacc attgttcaga taagtttaag caagatactg tcaatcacag
     3001 tgaccattca tggtcattac acttcctcca gttgccagac aatgcctaac ataaaaacgc
     3061 aaaagcccaa aagtcagagc ttaggtgttc taggcgtttt ctaagcgctg aagaaatacg
     3121 tcttaccgta gattgcgtgc gtttctgaag cacattttcc ttatacatgg ctttatccca
     3181 ggcagcttca tcaagctctt ggagtaacaa gccagtaata atgcgacact ctcgggtttg
     3241 tagcgagccg ctggtgagat cggagttata gcggaaaggg gtcatttgta attatttgtg
     3301 ggtggaggat agttaactta ttattgtagt tggaagaggc tgctttccaa acattagcac
     3361 ctcgtaatgc ttgttactgt atattaattt atgctcttag cgcatgttaa tgggctatta
     3421 caatgcataa tgtccacatc aagataactg tggcttaaca cctgtacaag cgaaaaaaat
     3481 caatcacctg tccggaaata cgggatcact gtaaagtgtt ggtgaggcag tctaggaggg
     3541 gga
//
LOCUS       DAJVQR010000205         3502 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVQR010000205 DAJVQR010000000
VERSION     DAJVQR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..320
                     /locus_tag="OQM52_004076"
     CDS             <1..320
                     /locus_tag="OQM52_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1565697.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYA
                     SMA"
     gene            477..1226
                     /locus_tag="OQM52_004077"
     CDS             477..1226
                     /locus_tag="OQM52_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1565698.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1248..1814
                     /gene="yieF"
                     /locus_tag="OQM52_004078"
                     /gene_synonym="chrR"
     CDS             1248..1814
                     /gene="yieF"
                     /locus_tag="OQM52_004078"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1565699.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1868..3205)
                     /gene="adeP"
                     /locus_tag="OQM52_004079"
     CDS             complement(1868..3205)
                     /gene="adeP"
                     /locus_tag="OQM52_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1565700.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3370..>3502
                     /locus_tag="OQM52_004080"
     CDS             3370..>3502
                     /locus_tag="OQM52_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS1565701.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     "
BASE COUNT          964 a          912 c          863 g          763 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactcca tcaactgccg ttggttgctc
      181 ttcctctccc gtttgacgaa agccagcaaa aaaagctgga agttcctttt accctgctgt
      241 ggcataaacg gaacagccat aatccgaaga tcgtctggtt acgggaaacc attaaaaacc
      301 tttacgcgtc gatggcataa ccgaatcgta tgaaacgcga cccaatttca caataaaatg
      361 taaaaaagtt gtaataagct tgtctgaatc gaacttttag ccgctttagt ctgtccatca
      421 ttccagtaaa tgattactct tgtattcata atggaccatt aagcatggag cgaaaaatgg
      481 cgactcactt tgcccgaggg attttaacgg aaggacatct gatttctgtt cgtctcccct
      541 cccagtgtca tcaagaagcc cgaaacattc cccctcatcg tcaaagccgt tttctggcgt
      601 ccagaggttt actcgcagaa ctgatgttca tgctgtatgg cattggcgaa ttgccggaaa
      661 tcgtcaccct gccgaaaggt aaaccggttt tcagtgataa aaatttgcct tcgttttcta
      721 tttcctatgc cgggaatatg gttggcgtgg cgttaacaac cgaaggtgaa tgtggcctcg
      781 atatggaact acagcgtgcg acgcgcgggt ttcatagccc acacgcgccc gataaccaca
      841 ccttttccag caatgaatcg ctatggatca gtaaacaaaa cgatcctaac gaagcgcggg
      901 cgcagctcat tacgctgcgc cgaagcgtgc taaaactaac cggtgatgtt ttgaatgacg
      961 atccgcgcga tctgcagctg ctgcccattg ccggacgcct gaaatgtgct catgtaaatc
     1021 atgtagaagc gttatgcgac gcggaggacg tgctggtgtg gtccgtggcg gtcacgcccg
     1081 cgattgaaaa gctcagtgtc tgggagttag atggcaaaca cagctggaaa agcctgccgg
     1141 atattcacag ccgcgccaac aatcctacca gccggatgat gcgttttgcc cagctctcta
     1201 ccgtgaagtc tttttcgcca aattgataga caaccacagg agtcattatg tctgaaaaat
     1261 tgcaggtggt tacgttactg gggagcctgc gcaaaggctc atttaatggc atggttgcac
     1321 gtaccctgcc gaaaattgct ccggcgagca tggaagtcaa tgcgttacct tccattgccg
     1381 acattcccct gtatgacgct gacgtacagc aggaagaagg ttttccagca acggttgaag
     1441 ctctggcgga acagatccgt caggctgacg gtgtggtgat cgtcacgccg gaatataact
     1501 actcggtacc gggtgggctg aaaaatgcca tcgactggct ttcccgcctg ccggatcaac
     1561 cgctggccgg taaaccggtg ttgattcaga ccagctcaat gggcgtgatt ggcggcgcgc
     1621 gctgtcagta tcacctgcgc cagattctgg ttttcctcga tgcaatggtg atgaacaagc
     1681 cggaatttat gggcggcgtg attcagaaca aagttgatcc gcaaaccgga gaagtgattg
     1741 atcagggtac gctggaccac ctgaccgggc aattgaccgc atttggtgag tttattcagc
     1801 gagttaagat ctaaataaaa aacccgccag taatcatgca tggcgggttt ttaatgcgct
     1861 atcgatttta atgagcgtcg ataaatacaa tcttcaggat aaacagcagc gcaacgatga
     1921 ttacgcacgg gctaaggtca cgcagacgcc cggtaccaat cttcatcacg cagtaggaga
     1981 taaagcccag cgcaatacct tcggtaatcg aaaagctgaa cggcatcatc acggcggtaa
     2041 taaacgccgg aacagattca gtaagatcct gccagttcac gcgagcaaga cttgaggtca
     2101 tcaacacgcc aacgtagatc aacgcgccag ctgcagcgta gtctggcacc attcccgcca
     2161 gcggcgacag aaagataacc agcaggaaca gcagaccaac aaccactgcc gtcagaccgg
     2221 tacgaccgcc aaccgatacg ccggaagagg actcaatata agccgtaacg gaagaagtac
     2281 cgataaacga accggtcacg gaagagatac tgtcgacata cagcgcctgc ttcatgcgcg
     2341 ggaatttccc cttctcatcc gccagacctg ctttatcggt cacgccaatc agcgtaccgg
     2401 aggagtcaaa caagttgacc aacatgaaag agaaaatcac ccctgccagc ccgaggttaa
     2461 acgacccggc taaatctaca tgacccacaa ctgtcattac gctcggcggc gcagaaacga
     2521 tgccattgta gtgcacatca cccagcatcc agcccagcag cgtcgtcacc acgatagaaa
     2581 ccagcaccgc tgcgtgaatg ttgcgcgagg ccagaatagc aatgatgaaa aagccgagga
     2641 tacccagaag tacgctgtga gaagtcagat taccgatgct caccagcgtt tccgagttag
     2701 cgacaatcac acctgcgttt ttcagcccca tcatgccgat aaacaggccg ataccgctgg
     2761 taatacccac acgcagactt accggaatgt tagcaatcat ccagtaacga acgcggaaaa
     2821 tggtcaaaag aagcagtccg acagcgcccc agaagattgc gcccataccg atctgccacg
     2881 gcagccccat tgcctggaca accacaaaag cgaaaaaggc gttaagcccc atcgcaggag
     2941 ccagcgccac cggaaggttt gcaaacaatc ccatcataat gctaccgaaa gcagcaatca
     3001 gacaggttgt tacgaagacg gcactggtgt ccatgccagc aacgcccaga atttgtgggt
     3061 taacaaatac gatgtagacc atcgtcagga aggtggtgaa cccggcaatc acttcagttc
     3121 gtgccgtagt accatgttcg cgcagtttaa aaacacgttc cagcatcctc tgaccagaag
     3181 tctgggtggt atgttgatga ctcattatct atttccgaac aaggagggaa aatccgtcgc
     3241 tatcgtatac caaaatgcga caataggcgc gtttgtgaga gactttttta ttggatttac
     3301 ttatacggca acgattgcgt tgcgcaaatc ggctctacga aaacgttaaa ctgattaaaa
     3361 aggaaaggca tgtcccggat agaagcggta tttttcgact gcgacggtac gctggtcgac
     3421 agtgaagtca tttgctcccg cgcatatgta acgatgtttc aggaatttgg tattacgctc
     3481 gatcctgaag aggtattcaa ac
//
LOCUS       DAJVQR010000206         3489 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVQR010000206 DAJVQR010000000
VERSION     DAJVQR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..389
                     /locus_tag="OQM52_004081"
     CDS             <1..389
                     /locus_tag="OQM52_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCS1565702.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OQM52_004082"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OQM52_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1565703.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="OQM52_004083"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="OQM52_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1565704.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OQM52_004084"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OQM52_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1565705.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3116..3487
                     /locus_tag="OQM52_004085"
     CDS             3116..3487
                     /locus_tag="OQM52_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1565706.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          949 a          641 c          716 g         1183 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgaga
//
LOCUS       DAJVQR010000207         3355 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVQR010000207 DAJVQR010000000
VERSION     DAJVQR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            483..1310
                     /locus_tag="OQM52_004086"
     CDS             483..1310
                     /locus_tag="OQM52_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1565707.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1354..2103
                     /locus_tag="OQM52_004087"
     CDS             1354..2103
                     /locus_tag="OQM52_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1565708.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2100..2669
                     /locus_tag="OQM52_004088"
     CDS             2100..2669
                     /locus_tag="OQM52_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565709.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2793..3035
                     /locus_tag="OQM52_004089"
     CDS             2793..3035
                     /locus_tag="OQM52_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1565710.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3039..3185
                     /locus_tag="OQM52_004090"
     CDS             3039..3185
                     /locus_tag="OQM52_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565711.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3194..>3355
                     /gene="xisR"
                     /locus_tag="OQM52_004091"
     CDS             3194..>3355
                     /gene="xisR"
                     /locus_tag="OQM52_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCS1565712.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          935 a          807 c          859 g          754 t
ORIGIN      
        1 agtgtccgct attgccagta cacctcacaa cgccatcctg acaagcgttt tatcttcaac
       61 gggaatgaga tatggaaacg tggagaatca accacagagt atacccgcgc acaccagcga
      121 acaatcagac gactgcgcag actcatcgcc aggaaacagt atacagaaaa atggttcaga
      181 aaatatgaca catggagcgc cggatattac gaactgatgg caacaaaacc attctgacgt
      241 aaacgaaatt aaccatgacg caattaaata aggcaagccg aatacatcag gaggaccatg
      301 aacatttatt tcagaatagt tatatcactg gcaattatcg catgtattta cggattaatc
      361 gttccggccc tcatatcaat gaaggatacg gtagcagtga tttctggctt tgctctggcg
      421 tgtctgaccc cgccctgcat ttatgccatt tataagggtc tttctttttc taaggataaa
      481 agatgaaaaa agttattcct gctttagccc ttgttctgct gaccaccgga cttgtcggtt
      541 gcgatcgcgt tgagcctggt aatgttggca tcaaggtaaa caaactgggc gacgataaag
      601 gcgtcggaga agtggtcggt gttgggcgct actggacggg atggaacact gaggtttaca
      661 tcttcccgac cttcaaacaa atgaagacct acgatgagcc attcagcttc cagatgagtg
      721 acggtacaac catcggctat cacatcggcg tggcctacaa agttgatcca tccaaagtta
      781 ccacagtctt tcagacctac cgcaaaggcg tggacgacat taccgacacc gacctgcgcc
      841 agaagatagc cgacgcactc aaccgactgg ccagcaaaat gaccaccgac aaatttatcg
      901 acggcggcaa gtctgagctg ctggatgcag ctcttaaaga cattcaggaa gaaatgacgc
      961 ccatcggtat tcaggtaatg agcctctcat atgtgggtaa gccggagtac ccgcctactg
     1021 ttatcgacag cattaatgcc aaagtcacgg cgaaccagaa aaccctgcaa tgcgagcagg
     1081 aagtaaaaca gcgcgaagcg gaagccaaca tgttgcgcgc ggaagctgcc ggacaggcag
     1141 atgcgattcg cacaaaagcc caggccgaag ccgatgctat tcgtttacgc ggtgaagctc
     1201 tgcgccagaa cccaaacgtt atggagctgg aagccatcaa caagtggaac ggtacactgc
     1261 cgcaatacat gaccagcggt gccaacacac catttattca ggttaagtaa tacatatgcc
     1321 cggtattaca cgccgggctg tctggagata aaaatgaata ctgtaaccat caacaacaaa
     1381 cagtttccgg taatcgaata tcgcggtcag cgtgttgtga cgctggcgat gattgatgaa
     1441 gtccaccagc gaccggaagg aaccgcgcgt gcagcattca accgaaaccg tgagcatttc
     1501 atcagcggtg tggattatgc cgaattaggt gcggacgtaa tacgtacgga cctcccggaa
     1561 ggaacattct ctaaatttgc accatcgggg attgtacttt ttgaatcagg ttacctgatg
     1621 ttgacgaagc cattcaatga cgcccttgcc tggcaggttc agcgcgaact ggttaacagc
     1681 tacttccgca ctcgcacacc gctaacggaa atcgagatga tcgctgcgat agccgccgac
     1741 gctgttcgtc aacagaagcg cctgaatcat gtggaagaac agctcgaaac agtcacagaa
     1801 gcggtggaaa ccatcaaacg cggaaatatg cgcgccggat atgtcggtta ccgccaggtg
     1861 gtagccaaaa gtggaatgag tgacaccaag tgtcggaatc tggtgaacgc atatcaaatt
     1921 cctacagaca cccacgaatt catgactccg gacggtctgt tgtctcgcag ggctatcgtc
     1981 gagcttgagc catttatggc ggcgttccac caaatgatgt cagaggctga cccacgcggc
     2041 acacgctggt atcacccgaa gatggggctt ttccaggtga tcggatggga ggataaagca
     2101 tgatcatcca gtcaaaactt attcgcgccg ctctggtgtg cgctgctaaa aacgacgttc
     2161 gttattacct gaacggtctt cacatcacgc cgaaacatat tgaggcaacc aatggttccg
     2221 tggcactgcg catggctcac ggcatccgga cgaagaaaaa catcattgtc cagttcgaag
     2281 ggggcgtccc ggccaaagcc gaaacgacag agctgatttt tagtaaagag ccgatcgctg
     2341 ttcatcgcga ccagtttcag cgacgactgt ccattaccgg cattaaactg gtggacggtt
     2401 gttttccgga tttggatcgc atcattccga aaaagtttga ccgctgtact cacccggtgc
     2461 tccaggcggg ttacctgagt tatccagaga agatgtttgg tcgtgagcgt aaatttattc
     2521 ccgtccagtt acgtccctcc ggtgacgggc aagcggtcag aattcagttt gattccatca
     2581 tcaactcaat gtatggcaat cctgaatttg ttgtgatgcc ttgtcgtgat catggcgatt
     2641 tcaacgtggc tcaggagcat ccggaatgaa aatcgaatac caggacgccg caggaggtga
     2701 atcaatgagc tggcctgatg caatcgtaac tctggggatg gtattcgcag tagcgtttgt
     2761 tgtgtactcg atttgtcgat ggggataacc acatgttcgc tttgattcaa cgcgggcaga
     2821 tatacacaga cagagccgga tacccagtag aaattactcg cagtactgag cactcagtgt
     2881 tctttcggag gatggacgga cgtaccgggc gagtacgcat cggtgaattc agtagcctgt
     2941 tcgagcatat tgaccacctg gaatatcaca agattctggc tgaaacagaa caagaaaagc
     3001 acctgaagaa attacgcgca atgcaaagga agtaaaaaat gaataaagcg tttgagctat
     3061 gggtccacca gcgttacggc aaccactacg acctgacgcg agatgttgac ggtttctact
     3121 gtcgtgaaat tgtgaaacga atgtttgagg tctggtgcca ctgccgtggg ctgagtgttg
     3181 tgtgaggtaa tgcatgggca atgtgattca actggctccc aatgaatggg tttgtgaaag
     3241 cgttctaatc gcaattaccg ggctcaaacc aggcacaatt cttcggaccc ggaaagaatg
     3301 ctggatggtt ggaagagagt atattcacgt atcaccagac ggtaatccaa agcct
//
LOCUS       DAJVQR010000208         3328 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVQR010000208 DAJVQR010000000
VERSION     DAJVQR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            9..887
                     /locus_tag="OQM52_004092"
     CDS             9..887
                     /locus_tag="OQM52_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1565713.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            884..2275
                     /locus_tag="OQM52_004093"
     CDS             884..2275
                     /locus_tag="OQM52_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1565714.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2272..2529
                     /locus_tag="OQM52_004094"
     CDS             2272..2529
                     /locus_tag="OQM52_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565715.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2624..2824
                     /locus_tag="OQM52_004095"
     CDS             2624..2824
                     /locus_tag="OQM52_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCS1565716.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2817..3197
                     /locus_tag="OQM52_004096"
     CDS             2817..3197
                     /locus_tag="OQM52_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1565717.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          879 a          722 c          964 g          763 t
ORIGIN      
        1 tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag gcgcgaatgg
       61 atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg ccttatcgtt
      121 cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat cacacgcata
      181 tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc tgccctgact
      241 gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag gcggaagagc
      301 tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat aattatctgg
      361 aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg gagaactggc
      421 cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt accggaaaaa
      481 atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc agtgtggaga
      541 tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat gacagcgaaa
      601 aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc atcgacgaag
      661 ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt atcaacgcca
      721 ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa gagttgtggg
      781 cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac tggttgtcgt
      841 ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa ctccagtatg
      901 gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg gagcagatcc
      961 tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac gagcgtatca
     1021 ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg accccgtgct
     1081 gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc gtaaaacctg
     1141 ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg cactgcgcga
     1201 tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata cctgtgaagc
     1261 agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga caggaaaggg
     1321 cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc gtgttgaatg
     1381 ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg aactggacgc
     1441 aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc cttcgatggg
     1501 gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc atggtgtgct
     1561 tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt ctgcggctgg
     1621 agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag actggacgcg
     1681 tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg accaggcgaa
     1741 cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat atccggaaac
     1801 ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg ggcgtcatga
     1861 ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg gtggttttcc
     1921 gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata aacgtcccat
     1981 gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca ttctgatgct
     2041 ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag aaatcaatat
     2101 cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata acggacattt
     2161 tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc ccggcaatcc
     2221 gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct tttgaacaac
     2281 cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat gctgcgtcat
     2341 gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga tatggtaccc
     2401 gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga tgagctggtg
     2461 gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt gcaggaggcg
     2521 ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat tatgtccctg
     2581 gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg tcaaaattca
     2641 aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt cacgaatgct
     2701 gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca aagggcacgc
     2761 cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag acagtgatgc
     2821 gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat catgaggatg
     2881 tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa gtaaaatccc
     2941 gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc cgccttaacc
     3001 ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg atgacattca
     3061 tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag ctacaaaaag
     3121 ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa atggacaaat
     3181 atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac gtacgtaaaa
     3241 aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg cctccgggcg
     3301 ggttttttat gcctgaaaaa cggtacag
//
LOCUS       DAJVQR010000209         3258 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVQR010000209 DAJVQR010000000
VERSION     DAJVQR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..442
                     /locus_tag="OQM52_004097"
                     /pseudo
     CDS             1..442
                     /locus_tag="OQM52_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OQM52_004098"
     CDS             1026..1742
                     /locus_tag="OQM52_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565718.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OQM52_004099"
     CDS             2294..2698
                     /locus_tag="OQM52_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565719.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="OQM52_004100"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="OQM52_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DAJVQR010000210         3180 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVQR010000210 DAJVQR010000000
VERSION     DAJVQR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OQM52_004101"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OQM52_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1565720.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OQM52_004102"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OQM52_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAJVQR010000211         3112 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVQR010000211 DAJVQR010000000
VERSION     DAJVQR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OQM52_004103"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OQM52_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1565721.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OQM52_004104"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OQM52_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OQM52_004105"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OQM52_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCS1565722.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OQM52_004106"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OQM52_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCS1565723.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="OQM52_004107"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="OQM52_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJVQR010000212         3098 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVQR010000212 DAJVQR010000000
VERSION     DAJVQR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(105..1814)
                     /locus_tag="OQM52_004108"
     CDS             complement(105..1814)
                     /locus_tag="OQM52_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCS1565724.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2246..2965)
                     /locus_tag="OQM52_004109"
     CDS             complement(2246..2965)
                     /locus_tag="OQM52_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCS1565725.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1038 t
ORIGIN      
        1 tgccaattga ggtggttgcc tgaaagttgg cagttacccg gtcactacac cttgttggag
       61 tgaacatatc aaaaacaggt aattacacaa tttaaattac agtctcataa aaaatgatta
      121 aatctattta ttatttcaat aaacgttttt ttcatcttct ttgaaaattg ttctgctata
      181 gtatccggtt ccagattgtt ctcttttatt attctacaat actctaatat tgtttctttg
      241 tctctattta ctacagatac aaatacacca ggaataccat ctttattagt tgcggacagc
      301 atttcgacca atcgtcttgt ggtaaaacta tagtttttta gaatattaca aaaggcacgt
      361 attgtttcgg tatggccatt ataaaatgcc ataaaaagtc ctgatgtacc gttgggattt
      421 tttatccata ataattcatc tatatattcc tggtgagggg ggagtatttt tagtatatct
      481 ccatacgcca taatggcctt tgtgtgccca ttttgaagtg ccataaacaa cccatgcttt
      541 actttcaata attctttaat ttgttcacta ctaagcattt tcttattaag cagacttccg
      601 aacatacgaa tactatctgc atgtccattc tgcaatgcaa aaaataaccc aggtaaatca
      661 tggctgtctt tcgcagctaa tagatataat ctcttatcgg aagttaacgg ggttttaaag
      721 actatttccc cataggcttt tattgtgtct acatgtccgt tcataaaaga catgcataaa
      781 cccggcacat tgtttgcccc taccgcagca agcaatgtaa gtattgtttc ttgactaaga
      841 ttgcacatat ttaatatctt accatatact tgaataatgt ctgaatatcc gttttgaagt
      901 gctatataaa atgcaggagt accatcagag cttctcgtgg ccagaaggtt caccagttta
      961 gattgagaaa tttcaccatt tttgtatttt tccatcagta taaataatac ttctgtcaga
     1021 ttctttgtta tcgcatgata tataactctg gaattgatca attcatctgg taaaagtttc
     1081 actgtgtttg ttagagggga attatgccta tcttcaaata ttacctgccc gggttgctcc
     1141 agaccataaa ttttccaata aaaatcatca aaaaaatcct tagcggataa ttgactcagt
     1201 tcgaaatggc tatcacaagt aaatacagta cgtttatgag taacagttcg attaggatcg
     1261 aaaaactgaa caacccattt ttcactttct tgtacgcgct ttattttcaa ccctaacgcc
     1321 atttggtggt ttaatgtgca tgctgataat gttctgacaa agtctccatt ttttttcatg
     1381 ttataaaaaa aattagtaat aactgttccc cactttttat tttccacaaa ataaatataa
     1441 gctggtgatt ttgaaaaatc ttccgttttt tccaaacata cattttttgc aatagcatca
     1501 ggggtgctaa gaagctgata gtttaccttc ccatttgttt ccataaaatt catttcccaa
     1561 taggaagcta ggtgacggca aactatttgt cttccatata tatcttttgc cactccattt
     1621 atatttacct ccacatttct cttttctgaa aaataaggtg ctgttttatt cttaaactga
     1681 attagttcac tggagtttga gaccctcata atagaacttg ttattgttct attatctctg
     1741 ttaacagaca tatctgttga taacgaattt tttgttgaaa atatactaga tgaaaatgtt
     1801 ttatttaacg gcatagaaga caaccataga gatgttataa tactattaga ttgcagttac
     1861 taatagtaaa agcctcctta caaggaggta gcttattcag aatgctccta taagatagcg
     1921 agctcgtcaa cgatacagat gatctgatta tattgtgcaa aaggagaaca ggaaaatagg
     1981 cactggaaaa gatgaaggcc tgatgggagc attaactaat gcaaaatccg acaggttaat
     2041 atcccgaaaa gcaatcaaac gactcaaaca ggagatgcac gctgaaatgg tggacaactg
     2101 ttcggatttc agatgagtag ctcagcagtt tatgagccga atgctctgtc acgggcagga
     2161 atcaggctgc atacgcagtg ccaaatctaa tcgtagttat tctaaccagt tttcaagcta
     2221 caaggtggtg tagctggcat cttctctact ctatcatcaa acgataaaat ggttcctctc
     2281 gtaacatttg ctcttgcctt tcacttccat ctcgatcgct tcgtagttca ttacgatagc
     2341 tgacatattt ccagtcttca ggtctaacat ctgacgccgg atactccccg ggagcgattt
     2401 tgcttcttaa tagattagac tcgagacaca ttataaattg tcgtatttta tgaagtaata
     2461 aagcactata ttgcgagcat tcctgatctg attttacata tagcgtaaac tgagctccta
     2521 tccccacacg agattgctga gaaactcgat tcatatcagt tatcttccat ttatctatag
     2581 gactgtcctc tgaaaataat aaaccagaca aaatttggaa tgccagagga acttgctccc
     2641 tagctatact aatatgaaac ttgtccccaa caaaatcccc cttactctga ggattttcac
     2701 gacatgcatg aataaacaca tctccactat ggttcacaat ctggaatcct tggtacatga
     2761 cagaaaaaga ttgacatata tcagagtaag ttggcacgtt cgatgctact ttgaaatagt
     2821 acgctgggta ttgccgtatt tggttgtaca atatattgaa gttatttttc aatctttcat
     2881 tcgcagaaag catctgcggt atttcagacc tcacaacatt caaagaatct aaatttagtt
     2941 ttagacaggg cttttttatg ggcatagaaa acgtcctcta taaaatagat atattatctt
     3001 atattgattg tttatcatct tttataaaac gatacattat atgtaatgta tgtaagcgta
     3061 agggtcctct ccgaacatgg tgggaaggct tagaacgc
//
LOCUS       DAJVQR010000213         3095 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVQR010000213 DAJVQR010000000
VERSION     DAJVQR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            2..2905
                     /locus_tag="OQM52_004110"
     rRNA            2..2905
                     /locus_tag="OQM52_004110"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3001..>3095
                     /gene="rrf"
                     /locus_tag="OQM52_004111"
     rRNA            3001..>3095
                     /gene="rrf"
                     /locus_tag="OQM52_004111"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          815 a          684 c          967 g          629 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     3061 cgtagcgccg atggtagtgt ggggtctccc catgc
//
LOCUS       DAJVQR010000214         3009 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVQR010000214 DAJVQR010000000
VERSION     DAJVQR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            102..740
                     /locus_tag="OQM52_004112"
     CDS             102..740
                     /locus_tag="OQM52_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1565726.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            743..1906
                     /locus_tag="OQM52_004113"
     CDS             743..1906
                     /locus_tag="OQM52_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1565727.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1990..>3009
                     /locus_tag="OQM52_004114"
     CDS             1990..>3009
                     /locus_tag="OQM52_004114"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1565728.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHV"
BASE COUNT          679 a          718 c          858 g          754 t
ORIGIN      
        1 aaattattaa tcacaatacc gcagcaggaa tgtttatggg aaccgccgcg ctggctggcg
       61 gtatttttaa cgcagcttgt atgacatggt aatggacgat catggccaga aaacgcaaat
      121 caagaaataa cagtaaaatt ggtcacggag cgatcagtcg cattggtaga ccgaataatc
      181 cttttgaacc gcgtcgcaat cgctacgcac aaaaatactt aacgttggcg ctaatgggcg
      241 gtgccgcttt tttcgtattg aaaggttgta gcgatagtag cgatgtcgat aacgacggcg
      301 acggaacatt ttacgcgaca gtgcaggatt gtattgatga cggtaataat gccgacattt
      361 gcgcgcgtgg ctggaacaac gccaaagcgg cattttatgc cgatgttcca aagaatatga
      421 ctcagcagaa ctgtcagtct aagtacgaaa attgctacta cgacaatgtt gaacagagtt
      481 ggatcccggt cgtttctgga tttttgttaa gccgggttat tcgtaaagat cgcgatgagc
      541 cgttcgtcta taacagcggc ggatcctcat ttgcttcccg ccccgtctgg cgcaacactt
      601 ccggtgatta ctcctggcgc ttcggttctg gcaaaaaaga gtcttactct tcaggcggct
      661 ttaccaccag aaaagcgtcg accgtttcgc gcggcggcta tggtcgttct tccagcgccc
      721 gtgggcattg gggaggctaa tcatgctgag acacaacgtt cctgtgcggc gggatctgga
      781 ccagatcgcc gctaacaacg gtttcgactt tcatatcatc gacaatgaaa tctattggga
      841 cgagagtcgg gcttaccgtt ttactctgcg ccagattgaa gagcagatcg aaaaaccgac
      901 tgcggaactg catcagatgt gccttgaggt ggtggatcgc gcggttaaag acgaagagat
      961 cctgacgcaa ctggcgatcc cgccgttgta ctgggatgtg atcgccgaaa gctggcgcgc
     1021 acgcgatcct tcgctgtatg gccggatgga ttttgcctgg tgtggcaatg cgccggtgaa
     1081 gctgctggag tacaacgccg atacgccgac ctcgttgtat gagtcggctt atttccagtg
     1141 gttgtggctg gaggatgccc ggcgcagcgg cattattccg cgtgatgccg atcagtacaa
     1201 cgccattcag gaacgcctga tttcgcgctt tagtgagctt tacagtcggg aactgtttta
     1261 tttttgctgc tgtcaggaca ccgatgaaga caggagtacc gtgctgtact tgcaggactg
     1321 cgcccagcag gcagggcagg agtcgcggtt tatctacatt gaagatctcg gtttgggcgt
     1381 cggcggcgta ctgaccgatc ttgatgataa tgtcatccag cgtgcattta agctgtatcc
     1441 gctggagtgg atgatgcgtg acgataacgg tccgctgctg cgcaagcgtc gcgagcaatg
     1501 ggtggagccg ttatggaaaa gtatcttgag taataaaggg ctaatgccgc tgctttggcg
     1561 cttcttccct ggtcatccta accttcttgc atcctggttt gagggtgaaa aatcgcagat
     1621 tgccgctggc gaaagctatg tgcgtaaacc gatctactcg cgcgaaggcg gaaacgtcac
     1681 cattttcgac ggtcagaata acgtcgttga ccacgctgat ggtgattacg ccgatgaacc
     1741 gatgatctac caggcgtttc aacctctgcc gcgatttggc gatagctaca cactcatcgg
     1801 tagctggatt gtcgatgatg aagcgtgcgg aatggggatc cgtgaagata acacactgat
     1861 caccaaagac acctcacgtt tcgttccgca ttacattgct ggataagaat gttttagcaa
     1921 tctctttctg tcatgaatcc atggcagtga ccatactaat ggtgactgcc attgatggag
     1981 ggagacacag tgcactggca aactcacacc atttttaatc aacctatacc attaaataac
     2041 agcaacttat acctgtctga tggcgcgctt tgcgaagcgg taacgcgtga aggtgctggc
     2101 tgggatagcg attttctagc cagtattggt cagcagttag gaacggctga atcccttgaa
     2161 ctggggcggc tggcgaatgt aaacccgcct gaactgttgc gctacgatgc ccaggggcgg
     2221 cgtctggacg atgttcgttt ccatcccgca tggcacctgc tgatgcaggc gttatgtacc
     2281 aatcgggtgc acaatcttgc ctgggaagaa gacgctcgct ccggcgcatt tgtggcgcgc
     2341 gcggcgcgtt ttatgttaca tgcgcaggtt gaggctgggt cgttatgtcc agtaacgatg
     2401 acctttgccg ccacgccatt gttgttacag atgttaccca cgccgtttca ggactggacc
     2461 acgccgctgc tgagcgatcg ctacgattct cacttattgc caggtgggca aaaacgcggt
     2521 ttgttgattg gcatgggaat gacggaaaag cagggcggtt ccgatgtcat gagcaacacc
     2581 acccgtgcag agcgtttgga agatggctct tatcggctgg tggggcataa atggtttttc
     2641 tcggtgccgc aaagcgatgc gcatctggtg ctggcgcaga ccgcgggcgg tctgtcctgc
     2701 ttttttgtgc cgcgcttttt gcctgacggg caacgcaacg cgattcgcct cgagcggctg
     2761 aaagataagc tgggtaatcg ctctaacgcc agttgcgaag tggagtttca ggatgccatt
     2821 ggctggttat tggggcagga aggggaagga attcgtctga tcctgaaaat gggggggatg
     2881 acgcgttttg attgcgccct gggtagccat gccatgatgc gccgtgcatt ttcgctggcg
     2941 atttatcatg cacatcaacg ccatgttttt ggtaatccat tgatccaaca gccccttatg
     3001 cgtcatgtc
//
LOCUS       DAJVQR010000215         2963 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVQR010000215 DAJVQR010000000
VERSION     DAJVQR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..187
                     /locus_tag="OQM52_004115"
     CDS             <1..187
                     /locus_tag="OQM52_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1565729.1"
                     /translation="GGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGV
                     NVFIQDVPGDQKEQIPD"
     gene            226..1029
                     /gene="agaC"
                     /locus_tag="OQM52_004116"
     CDS             226..1029
                     /gene="agaC"
                     /locus_tag="OQM52_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCS1565730.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1019..1810
                     /gene="agaD"
                     /locus_tag="OQM52_004117"
     CDS             1019..1810
                     /gene="agaD"
                     /locus_tag="OQM52_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCS1565731.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1811..2566
                     /locus_tag="OQM52_004118"
     CDS             1811..2566
                     /locus_tag="OQM52_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCS1565732.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          765 a          668 c          703 g          827 t
ORIGIN      
        1 aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg aagggaaaaa
       61 gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac gttttattaa
      121 acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag aacaaatccc
      181 tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca tgaaataacc
      241 ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga tttttggctg
      301 gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc tattctcggt
      361 gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc cggattaacc
      421 cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac caccgtcatt
      481 gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc gtttagtttg
      541 ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat gaccaaagcc
      601 gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg gacaacgatg
      661 ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct ggcacaaggg
      721 gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt tgaagtggct
      781 ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt caaagcgcaa
      841 tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt cagcaacctg
      901 ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa tgcgaaatcc
      961 cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga ctacagcaat
     1021 gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt cgctgctgca
     1081 agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga tgttgccgat
     1141 cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga aagataacct
     1201 cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat taatttcgat
     1261 ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac tgtttggccc
     1321 aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta tggcgggaat
     1381 ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt tcgccgttta
     1441 cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag tcggcgtgaa
     1501 ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa ccatcctcgg
     1561 gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg tgacatcgtt
     1621 tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata aagtcttccc
     1681 gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg tgaaaaaagc
     1741 gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt ccgcattcgg
     1801 cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta aggaatttca
     1861 tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac gagccagcga
     1921 atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg cgaccggagc
     1981 cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc aggttgatat
     2041 cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa cgatgcctgg
     2101 cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac gtgaagacca
     2161 gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg taacgaacct
     2221 gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa acggtcatct
     2281 tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc aacttgatgc
     2341 cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc gtgggatcac
     2401 cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga ctggcgaagg
     2461 aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta tcccggcttc
     2521 atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag atgaaaaatt
     2581 tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa taacatttga
     2641 ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta attttagata
     2701 tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc ctggcgcagc
     2761 aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata taataaaaac
     2821 ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt tcaatgaaat
     2881 atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa tatgattatg
     2941 tacttgttac aaggataagg tta
//
LOCUS       DAJVQR010000216         2940 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVQR010000216 DAJVQR010000000
VERSION     DAJVQR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            130..1998
                     /gene="kup"
                     /locus_tag="OQM52_004119"
     CDS             130..1998
                     /gene="kup"
                     /locus_tag="OQM52_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1565733.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2165..2584
                     /gene="rbsD"
                     /locus_tag="OQM52_004120"
     CDS             2165..2584
                     /gene="rbsD"
                     /locus_tag="OQM52_004120"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1565734.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2592..>2940
                     /locus_tag="OQM52_004121"
     CDS             2592..>2940
                     /locus_tag="OQM52_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1565735.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          656 a          700 c          772 g          812 t
ORIGIN      
        1 ttttctttga gccaggttaa tgtggccgca tttaggagta cgattttgcc gttaatcgtg
       61 catactgtgc gcttttttgt gggccaaggg actaagcaca catttcatat ttcaacgaaa
      121 gactagtcta tgagcactga taataagcaa tcattgcccg cgattaccct cgcggcgatt
      181 ggagttgtct acggcgatat tggtaccagc ccgttatata cacttcgtga atgtttgtcc
      241 ggccagtttg gttttggcgt tgaacgcgat gccgtgtttg gctttttatc gctgatcttc
      301 tggctgctaa tctttgtggt ttccattaaa tatctcacct tcgtgatgcg ggcagataac
      361 gccggtgagg gggggatcct gacgttgatg tcgcttgccg ggcgtaatac gtcggcgcga
      421 accacatcaa tgctggtgat tatggggcta atcggcggca gctttttcta tggtgaagtc
      481 gtcataacac ccgctatttc ggtgatgtca gccattgaag gtctggaaat cgtcgccccg
      541 cagctggata cctggatagt tcccctctca attatcgttc tcactttatt atttatgatt
      601 caaaagcatg gcacggctat ggtcggtaag ctgtttgcgc cgatcatgct gacctggttt
      661 ttgattctgg caggactggg gttacgtagc attattgcta acccggaagt gctgcatgcg
      721 ctgaatccga tgtgggcggt gcatttcttc cttgaataca aaacggtttc ttttattgca
      781 ttaggggcag tggtgctgtc gattacgggg ggcgaggtgc tgtatgctga tatggggcac
      841 tttggtaagt tctctattcg cctggcgtgg ttcaccgtcg tattaccttc cttgaccctt
      901 aattacttcg gtcagggagc gttgttgtta aagaacccgg aagcgattaa gaacccgttc
      961 ttcctgttgg caccggactg ggcgctgatc ccactgctga tcatcgccgc actggcgacg
     1021 gtaattgcct cgcaggcggt tatctctggc gtcttctcat tgacgcgtca ggcggtacgt
     1081 ctgggatatt tgtcgccgat gcgcattatt cacacctccg aaatggagtc agggcaaatc
     1141 tatattccgt ttgtgaactg gatgctctat gtcgcggtcg tgattgtgat tgtcagcttt
     1201 gagcactcca gcaacctggc ggcggcgtac gggattgcgg tgaccggaac catggtactg
     1261 acgtctattc tctcgactac cgtggcacgt cagaactggc actggaataa gtattttgtt
     1321 gcgctgatcc tgattgcttt cctttgtgtc gatattccat tgttcaccgc taacctcgat
     1381 aaactgctct ccggcggctg gttgccattg agcctcggta ctgtgatgtt tatcgtgatg
     1441 accacctgga agagcgagcg tttccgcttg ctgcggcgga tgcatgaaca tggtaactct
     1501 ctggaagcga tgattgcttc gctggagaaa tcaccgcctg ttcgcgtgcc cgggaccgcg
     1561 gtgtatatgt cgcgtgcaat caacgtcatt ccctttgcgc tgatgcataa ccttaaacat
     1621 aacaaggtat tgcatgagcg ggtgattctg ttaactctgc gcaccgaaga cgcgccatat
     1681 gtccataacg tccgtcgggt acagattgaa caactgtcgc ccactttctg gcgcgtggtg
     1741 gcaagttatg gttggcgaga aacgccaaac gtggaagaag ttttccaccg ctgcggtctg
     1801 gaaggattaa gttgccggat gatggaaacc tccttcttta tgtcgcatga gtcgttgatc
     1861 ctcggcaaac gcccgtggta tttgcgtctg cgcggcaagc tgtacttgct gctgcaacgt
     1921 aatgcgctgc gtgcaccaga tcaatttgaa atcccgccaa acagggttat cgaactgggt
     1981 actcaggtcg aaatctaacg ccagacgcct cctttcttca taagggggcg tttttgtttt
     2041 catggttaat caccatgtaa aacgtttcga ggttgatcac atttccgtaa cgtcacgatg
     2101 gttttcccaa ctcagtcagg attaagctgc ggatcagcga aacgtttcgc tgatggagaa
     2161 aaaaatgaaa aaaggcaccg ttcttaattc tgatatttca tcggtgatct cccgtctggg
     2221 acataccgat acgctggtgg tgtgtgatgc tggtttaccc atccccaaaa gtacaacgcg
     2281 tatcgatatg gcattaaccc aaggtgtacc ttcttttatg caggtgctgg gcgtcgtcac
     2341 aaatgaaatg caggtcgagg ctgccattat cgcggaagag atcaaacaac ataatccgca
     2401 actccacgaa acgttgctca ctcaccttga gcagctgcaa aaacaccagg gaaataccat
     2461 tgaaattcgt tacaccacgc atgaacaatt caaacaacaa accgcagaaa gtcaggcggt
     2521 aattcgcagc ggagaatgtt ctccgtatgc gaatatcatt ctctgtgctg gcgtgacgtt
     2581 ctgaggccgt catggaagca ttacttcagc ttaaaggcat cgataaagcc ttcccgggcg
     2641 taaaagccct ctcgggcgca gcgttaaatg tctatccggg ccgtgtgatg gcgctggtgg
     2701 gcgaaaacgg cgcgggtaaa tccaccatga tgaaagtgct tactggtatc tatactcgcg
     2761 atgccggcac gcttttatgg ctggggaaag aaacgacatt taccgggccg aaatcttccc
     2821 aggaagccgg gattgggatt atccatcagg aactgaacct gatcccgcag ttgaccattg
     2881 ccgaaaacat tttcctcggt cgtgagtttg ttaatcgctt tggcaaaatt gactggaaaa
//
LOCUS       DAJVQR010000217         2885 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVQR010000217 DAJVQR010000000
VERSION     DAJVQR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..309)
                     /locus_tag="OQM52_004122"
     CDS             complement(<1..309)
                     /locus_tag="OQM52_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1565736.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     D"
     gene            complement(356..1075)
                     /gene="yehT"
                     /locus_tag="OQM52_004123"
     CDS             complement(356..1075)
                     /gene="yehT"
                     /locus_tag="OQM52_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1565737.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1072..2757)
                     /gene="btsS"
                     /locus_tag="OQM52_004124"
     CDS             complement(1072..2757)
                     /gene="btsS"
                     /locus_tag="OQM52_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1565738.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          745 a          770 c          657 g          713 t
ORIGIN      
        1 gtcatcggtt ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat
       61 gcgacgttcc ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag
      121 gccattgagg aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc
      181 tttacgtagc cagacggcga tctgttccgc ggtggcttcg acattatcca gcgccagaat
      241 acgcaccagg tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt
      301 acttatcatg aggccttcag gtgttgatga ggcaaaaagc cattttagca gtcttttaca
      361 ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat
      421 tacgcaaaat caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga
      481 ggttaaccag atactgacga tggcagcgca gtagtggtgt acgactttcc aaggtacgta
      541 atgtcaattc ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc
      601 gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac
      661 aagggataaa tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc
      721 gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca
      781 gataatcaaa ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa
      841 aaacaatata cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc
      901 gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc
      961 cttccacggc gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata
     1021 tacgcaggtt ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc
     1081 gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct
     1141 tattccatag tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag
     1201 cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa
     1261 atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt
     1321 cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg
     1381 ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc
     1441 aatttgcaga taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg
     1501 ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc
     1561 gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt
     1621 cacctgggcg tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata
     1681 ttgccctgca agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt
     1741 tattgaactg aataaccggt ttttggcttc atacaatttg atggtgccca tcacgcgctg
     1801 attttcgcca cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga
     1861 gcaacgataa ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt
     1921 taaggtgtaa gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt
     1981 aaaggccagc aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata
     2041 cagtacctga gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc
     2101 cgtcgaggct gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat
     2161 cgcgcgttta tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat
     2221 cattggcgca gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc
     2281 aaggatgatc agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg
     2341 gttaaagact ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag
     2401 caatccttca acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata
     2461 tcgatgtaag ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc
     2521 gcccattacc gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa
     2581 ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag
     2641 acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat
     2701 gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc
     2761 cgcaaaccac acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg
     2821 aactcttgca gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat
     2881 aaaat
//
LOCUS       DAJVQR010000218         2799 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVQR010000218 DAJVQR010000000
VERSION     DAJVQR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..528
                     /gene="ssuE"
                     /locus_tag="OQM52_004125"
     CDS             <1..528
                     /gene="ssuE"
                     /locus_tag="OQM52_004125"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1565739.1"
                     /translation="SSLLEYAREKLNGLDVEVYHWNLQNFAPEDLLYARFDSPALKTF
                     TEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLATGGTVAHLLAVD
                     YALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTHLDTALETFWQALHRRD
                     VQVPDLLSLRGNAHA"
     gene            521..1479
                     /gene="ssuA"
                     /locus_tag="OQM52_004126"
                     /pseudo
     CDS             521..1479
                     /gene="ssuA"
                     /locus_tag="OQM52_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
     gene            1476..2621
                     /gene="ssuD"
                     /locus_tag="OQM52_004127"
     CDS             1476..2621
                     /gene="ssuD"
                     /locus_tag="OQM52_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCS1565740.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2630..>2799
                     /locus_tag="OQM52_004128"
     CDS             2630..>2799
                     /locus_tag="OQM52_004128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCS1565741.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASG"
BASE COUNT          623 a          769 c          776 g          631 t
ORIGIN      
        1 agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tagatgtaga ggtttatcac
       61 tggaatctgc aaaacttcgc cccggaagat ctgctttatg ctcgtttcga tagtccggca
      121 ctcaagacct tcaccgaaca gctgcaacag gctgatgggc tgattgtcgc cacgcctgtg
      181 tataaagccg cctattccgg cgcgctgaaa accctgctcg acctgctgcc agagcgcgct
      241 ttgcaaggca aagtggtgct accgctggcg acgggcggta ccgtggccca tctgctggcg
      301 gtcgattatg cccttaaacc agttttaagc gcactgaaag ctcaggagat cctgcacggc
      361 gtgtttgccg atgactcaca attaattgat taccatcaca gaccccagtt cacgccaaat
      421 ctgcaaaccc atcttgatac cgcgctagaa actttctggc aggcattgca ccgccgcgat
      481 gttcaggttc ctgaccttct gtctctgcga ggtaatgccc atgcgtaaca tcattaaact
      541 ggcgctggcg ggattgctta gcgtctctac ttttgcggtt gctgcagaat cctcgcctga
      601 agcgttacgt ataggctatc agaaaggcag tattggtatg gtgctggcaa aagccaccag
      661 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      721 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      781 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      841 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      901 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      961 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
     1021 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
     1081 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
     1141 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
     1201 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
     1261 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
     1321 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
     1381 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1441 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1501 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1561 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1621 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1681 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1741 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1801 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1861 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1921 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1981 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     2041 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     2101 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     2161 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     2221 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     2281 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     2341 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     2401 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2461 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2521 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2581 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2641 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2701 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga
     2761 aggagtggtg acggcgttct ggacgctctc cgccagcgg
//
LOCUS       DAJVQR010000219         2739 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVQR010000219 DAJVQR010000000
VERSION     DAJVQR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            78..143
                     /locus_tag="OQM52_004129"
                     /pseudo
     CDS             78..143
                     /locus_tag="OQM52_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            371..619
                     /locus_tag="OQM52_004130"
     CDS             371..619
                     /locus_tag="OQM52_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1565742.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(735..1004)
                     /locus_tag="OQM52_004131"
     CDS             complement(735..1004)
                     /locus_tag="OQM52_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1565743.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1061..1729
                     /locus_tag="OQM52_004132"
     CDS             1061..1729
                     /locus_tag="OQM52_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1565744.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2045..>2739
                     /locus_tag="OQM52_004133"
     CDS             2045..>2739
                     /locus_tag="OQM52_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1565745.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          838 a          594 c          537 g          770 t
ORIGIN      
        1 aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac tgggacctcg
       61 cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata
      121 ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga agatcgtcca
      181 ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac
      241 gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca
      301 ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac tacagttaca
      361 ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta aaatgccaaa
      421 cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga
      481 tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac gcaccgcaga
      541 taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg agtctgctga
      601 cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat cattcaatac
      661 tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg
      721 tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc aggtgcagtt
      781 gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag caacgagttt
      841 tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat atgctcactg
      901 aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgctgtatc
      961 gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc ccttgtaatt
     1021 catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat taatgaaaaa
     1081 aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt atgactggtg
     1141 ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac accttcttcc
     1201 acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa ctgggtttta
     1261 ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg aagctagcct
     1321 gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta gccatgatgt
     1381 ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca tgttttacct
     1441 tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca atgcggcgca
     1501 ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg gagttgtgca
     1561 caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct tttcaaacac
     1621 aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga atgttaaaac
     1681 caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc atccaaccgc
     1741 agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt gcgactttac
     1801 ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata tttaactaac
     1861 cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag tatcgttttt
     1921 ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca
     1981 ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct tctgcgaggt
     2041 tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt tttacagaac
     2101 tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat ggactaaaaa
     2161 cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat gtctggagaa
     2221 cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac gattgcctga
     2281 caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg agctaccaag
     2341 ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg aattaccagt
     2401 gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg aattacctgc
     2461 gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga ggttaccaat
     2521 gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag attttccaga
     2581 aagagatgat catattataa gaatattctg gcttaatcag aaccggatca cggcaattcc
     2641 ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa atcaactatc
     2701 tcccagaata atgcaaactt tgttacaaca aaccgccca
//
LOCUS       DAJVQR010000220         2697 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVQR010000220 DAJVQR010000000
VERSION     DAJVQR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            53..2574
                     /locus_tag="OQM52_004134"
                     /pseudo
     CDS             53..2574
                     /locus_tag="OQM52_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            2585..>2697
                     /locus_tag="OQM52_004135"
     CDS             2585..>2697
                     /locus_tag="OQM52_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1565746.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFH"
BASE COUNT          725 a          581 c          675 g          716 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gccccaggtg tttatagcgt tgatattttt
      241 atcaacggta aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct
      301 gatctgcacg cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt
      361 gatgcgctca aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt
      421 attgaaggct catcctggca gtttgacagt gataaattgc agctgaatat atccattcac
      481 caaatctaca tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg
      541 attaatgcgc tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat
      601 ggttcacaag agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg
      661 tggcggctac gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc
      721 attagtacct ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat
      781 acgtggacgg cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact
      841 gataacgata tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca
      901 aagtccaacg ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt
      961 ccacaaggtg cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac
     1021 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg
     1081 gcgattctta aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat
     1141 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
     1201 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
     1261 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
     1321 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt
     1381 gacgacacag acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat
     1441 accctcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct
     1801 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
     1861 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
     1921 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
     1981 tacagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     2041 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     2101 gccgccaggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     2161 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     2221 tcgaagtcga atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca
     2281 gtggttaaag cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat
     2341 gcaaatggta aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca
     2401 gattcaggaa ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga
     2461 caggttacgt tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcattt
     2521 tcgacaccag aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat
     2581 ttttatgaac aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag
     2641 tgtagcactt gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacag
//
LOCUS       DAJVQR010000221         2683 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVQR010000221 DAJVQR010000000
VERSION     DAJVQR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="OQM52_004136"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="OQM52_004136"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1565747.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="OQM52_004137"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="OQM52_004137"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1565748.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          641 a          677 c          768 g          597 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc
      361 cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc
      481 tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag
      601 agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg cgcagaaatc
      721 cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac taaattgcag gccgctgacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc
     1441 tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc
     1681 gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa cctgtcgcac
     1741 taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct taccggcggg
     1801 tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga
     1921 acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga
     1981 aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag
     2041 cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcgct
     2161 gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc
     2281 ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
     2461 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cag
//
LOCUS       DAJVQR010000222         2646 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVQR010000222 DAJVQR010000000
VERSION     DAJVQR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..119
                     /locus_tag="OQM52_004138"
     CDS             <1..119
                     /locus_tag="OQM52_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1565749.1"
                     /translation="EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            177..533
                     /locus_tag="OQM52_004139"
     CDS             177..533
                     /locus_tag="OQM52_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565750.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            626..844
                     /locus_tag="OQM52_004140"
     CDS             626..844
                     /locus_tag="OQM52_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS1565751.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            846..1211
                     /locus_tag="OQM52_004141"
     CDS             846..1211
                     /locus_tag="OQM52_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1565752.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1208..1873
                     /locus_tag="OQM52_004142"
     CDS             1208..1873
                     /locus_tag="OQM52_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565753.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1873..2238
                     /locus_tag="OQM52_004143"
     CDS             1873..2238
                     /locus_tag="OQM52_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1565754.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          750 a          541 c          702 g          653 t
ORIGIN      
        1 gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg
       61 aagctatgca gcgtgtactg gctttctatc agggtaattt tcaggaggcg atactgtgaa
      121 tgaaattagc tatcaggctt caattaccgc tggcattcgc atcaaaggag aggagcatgg
      181 aaataaaacc agaagatgag ttaagcaata tcgttttatt tccgataaaa gaggatgacc
      241 ctcgtaatca ggttaatttt ctttataagc catcggaaag accatattgc catcacgcct
      301 ctgtacgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg
      361 agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa
      421 ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg
      481 agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt
      541 tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatcagg
      601 agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat atccgcgaaa
      661 gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga caccgatatc
      721 cccgcaaatt tttgtctgga taatcggaaa aataattgag ccagttattg aattgtataa
      781 cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt ataaggaaag
      841 ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc ggtggtcgct
      901 gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta aaaccggtca
      961 ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac atggtaccag
     1021 aatgccaccc atgcaatctg cacaagcatt gcagtagcct ggaagattat cgacgaatta
     1081 tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg
     1141 gattggttga aatgaaatct gacccggttg tgttctggtt tgaaatatat caagaagggg
     1201 ctacggcatg actactatta ccaaagagcg actgcaatgg ctggctaaca tttctggccg
     1261 cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta tcgctctggc
     1321 atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca tgtgtattga
     1381 tgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc tttatgctgc
     1441 cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga aggtgacgtt
     1501 tactcaagtt gaccaggaat ctaatacgtt cactgctata tatttttaca aagcggaagt
     1561 tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa caccgcttta
     1621 cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc
     1681 actgtcagat tcatcgcttg gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc
     1741 tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa
     1801 cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa
     1861 gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag
     1921 cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga aatcgtggaa
     1981 atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat aagctgtagt
     2041 gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg gaatgtattc
     2101 cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat ggcttttgtg
     2161 gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc ggaaccaccg
     2221 caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt gcgatggcgg
     2281 tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc ccggattgat
     2341 ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc tgttgttacc
     2401 atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag ataaattagc
     2461 ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg gtaagattgc
     2521 tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca gagaaaagcc
     2581 ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc aatctatgtc
     2641 tcacaa
//
LOCUS       DAJVQR010000223         2572 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVQR010000223 DAJVQR010000000
VERSION     DAJVQR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(64..822)
                     /locus_tag="OQM52_004144"
     CDS             complement(64..822)
                     /locus_tag="OQM52_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1565755.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OQM52_004145"
     CDS             complement(830..1933)
                     /locus_tag="OQM52_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1565756.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OQM52_004146"
     CDS             complement(1943..>2572)
                     /locus_tag="OQM52_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1565757.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJVQR010000224         2560 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVQR010000224 DAJVQR010000000
VERSION     DAJVQR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            79..774
                     /locus_tag="OQM52_004147"
     CDS             79..774
                     /locus_tag="OQM52_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1565758.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            803..>2560
                     /locus_tag="OQM52_004148"
     CDS             803..>2560
                     /locus_tag="OQM52_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1565759.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLL"
BASE COUNT          750 a          602 c          564 g          644 t
ORIGIN      
        1 acgttacggc gttatctgac ctttcagata acgccctttt ccttcctctt tctcgttgta
       61 tcaggttgaa aaataactat gtcaaaacga acattcgcgg tgatattaac cttgttgtgt
      121 agcttctgta tcggccaggc gcttgcaggg ggtatcgttt tacagcgaac gcgagtgatc
      181 tatgatgcca gccgcaaaga ggctgcgtta cctgtcgcaa acaaaggcgc agaaacgcct
      241 tatttactgc aatcatgggt agataatata gatggtaaaa gccgtgcccc atttattata
      301 accccaccgc tatttcgtct tgaggctagc gatgactcat cactgcgaat tattaaaaca
      361 gctgataacc tgcctgaaaa taaagagtcg ctgttctaca ttaatgttcg tgccattcca
      421 gcaaagaaaa aatcagatga tgttaatgct aacgagttga cgctggtatt taaaacgcgg
      481 gtcaaaatgt tttatcgccc cgcacacctg aagggacggg taaacgatgc gtggaaatca
      541 ctggaattta aacgtagtga ccattcactc aatatatata acccaactga atattacgtc
      601 gtatttgccg gactggcagt cgataaaacc gatctcacaa gcaaaattga atatatcgcg
      661 cccggagaac ataaacagtt accacttcca gcatctggcg gaaagaacgt gaaatgggct
      721 gcgatcaacg attatggcgg cagttccggg acagaaactc gtccactgca ataaaaaata
      781 taaaaacaca ggccatcagg gaatgccaca acgacaccac cagggacata aacgcacacc
      841 gaaacagttg gcgctcatta tcaaacgttg tttgccgatg gtgctcactg gcagcggcat
      901 gctttgcact accgctaacg ccgaagagta ttatttcgac cccattatgc tggaaactac
      961 aaaaagtggt atgcaaacaa ccgatctgtc acgtttttca aaaaaatacg cacaactacc
     1021 aggaacttat caggttgata tctggctgaa taaaaagaag gtttcacaga aaaaaattac
     1081 atttaccgcc aatgcagagc aacttctgca gccacagttt acggtagaac aactacgtga
     1141 gctgggtatt aaggtggatg aaatcccggc gctggctgaa aaagatgacg atagcgtgat
     1201 caactcgctt gaacaaatca ttcccggtac agctgctgaa tttgatttca atcatcagcg
     1261 acttaatttg agcattcccc aaattgcact gtaccgtgat gcaagaggtt acgtctcccc
     1321 ttctcgttgg gacgatggta taccaacgct gtttaccaac tactcgttta caggttctga
     1381 taaccgttac cgccagggca atcgtagcca acgacagtac ctgaatatgc aaaatggtgc
     1441 taattttggc ccctggcgat tacgtaacta ttctacgtgg acacgcaacg atcaggcgtc
     1501 aagctggaac actatcagta gttatttaca acgtgatatc aaggcgttga agtctcagtt
     1561 gcttctggga gaaagcgcca ccagcggcag tattttttcc agctacgcct ttactggcgt
     1621 gcaactcgct tccgacgata atatgttgcc aaacagccag cgcggatttg ccccaacggt
     1681 acgcggtatc gcaaacagta gtgcaatcgt gactatcagg caaaatggtt atgtgatcta
     1741 tcaaagcaac gtgccagcgg gtgcctttga aattaacgat ctctacccct cttccaacag
     1801 cggcgattta gaagtcacga ttgaagaaag tgacggtaca caacgtcgct ttatccagcc
     1861 ttattcttca ttacccatga tgcagcgacc tgggcatcta aaatatagcg cgaccgctgg
     1921 acgctatcgc gctgatgcaa acagtgatag caaggaaccc gaatttgctg aagccacggc
     1981 aatatatggt ttgaataata cttttacgct gtatggcggc ctgctcggtt ctgaagatta
     2041 ttatgcgctg gggatcggta tcggcggcac acttggcgca ctgggcgcgt tgtcgatgga
     2101 tatcaacaga gctgacaccc aattcgataa ccagcactct tttcatggct atcaatggcg
     2161 tactcagtac atcaaagata tcccggaaac caacaccaat atcgctgtta gctactatcg
     2221 ctataccaac gatggctatt ttagttttga tgaagccaat acccgcaatt ggggctataa
     2281 cagtcgccaa aaaagtgaaa ttcaattcaa catcagccag acaatatttg atggggtaag
     2341 tctgtatgcc tccggttcac agcaagacta ttggggcaat aacgagaaaa acagtaatat
     2401 ctctgttggg gtttccggcc agcaatgggg aattggttac agcctgaatt atcaatacag
     2461 ccgctacact gatgaaaata atgaccgtgc actctctttg aatctcagta ttccgttaga
     2521 acgctggtgg atttgcccct atatttccag acatctgtta
//
LOCUS       DAJVQR010000225         2466 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVQR010000225 DAJVQR010000000
VERSION     DAJVQR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(749..2353)
                     /locus_tag="OQM52_004149"
     CDS             complement(749..2353)
                     /locus_tag="OQM52_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1565760.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          575 c          529 g          714 t
ORIGIN      
        1 gagaagacct ataggtttgg tgattgatta ttataaatat catcacccct taaggtattc
       61 ccttagagtt aatctttaat tggtctttaa ttaaatcttt tatttaatac ttaaagttaa
      121 tctctaattg tgtctttaag ttaactttaa gtaatactat aagttaatct ttaattgtgt
      181 ctttaagtta actttaagtt aagcccctgt agggactaga gaccataatt aagatgattg
      241 tgtcttattg ctattcactt atcgactgac ccagagcaca caggaagcat tcactttaag
      301 tagaacgctt ggtgtctgta attggtcatc ccgcccctga aaggatgcga taagttacgg
      361 ttagttgtcc tagaagagaa aactttttgt gtcccttctc cctatagtga gtcgtattaa
      421 ttatccttga caagtagaga aaaccatgag tcaaaaacat gaccacacag gtctgtagta
      481 agtgtcatcc tagaagagaa aactttttgt gtcccttctc cctatagtga gtcgtattaa
      541 ttaccccttg acaagcagag aaaactatga gtcaaaaaca tgaccgcaca ggtctgtagt
      601 aagtgtcatc ctagaagaga aaactttttg tgtcccttct ccctatagtg agtcgtatta
      661 attatccctt gacaagtaga gaaaactatg agtcaaaaac atgaccacac aggtctgtag
      721 taagtgtcat cctagactat ggtctcctct aggtctcctt ataaacatca gccatcacat
      781 tgaccagcct atcaacctcg acctctttag cataacggtc gtacgtaata gaccctgaac
      841 tatgccccat gatcgcctgt gcatacgcca actcgacccc atgttgtttt aactgggccg
      901 caactgtatg tctaaacgag tgaaacgcta agccgctacg gtctacgagg actttaggca
      961 acaacgtccg attaaaccat ttactcacct gctcaccgta accgttcttc atgagacgca
     1021 gaccatcgaa caccatagcg tcatcaccgt cagcgcctct acgggtctct acaagcgcca
     1081 taaagtcagc caatataaaa ccataggccc catcgaccag aggcacacag cggtcactat
     1141 gagcgttctt gatgctctta ccgggtagac tactatcgtc ttcattgata tgaatataaa
     1201 cggttcccgc ctcagtggtc ctgacgtctt tgacctgtag ttgggctatt tcattgagtc
     1261 tcgccccagt gatagcagcc agggcggtaa cataataatg atacggctta ccagaggtct
     1321 tctgagagta tgccttagct gcgaccagta gttgccccac ctgctccgta gagaaggcgt
     1381 tacgggcccc tgaggcttta cgctgaggga ctttaagctc cagaccctca gtcatgttct
     1441 ttttaatcaa gtcattgcgc acagcccacc taaagacagc agccatctta ataagatact
     1501 tgttgttgat ggtaacgaca tccagacaat ctgtcttatc ttcacggctc aacaggtcag
     1561 caagtgggac atccttgaag cgctgcttac ggttcctagg taactgctgg agcacatcac
     1621 ggaccctaag catgtcagcc ctgttagcgt ctgcattgag acccagatgg tcgaatattt
     1681 cgattaacgc agcgtgtgac gccttgtttt cccttaaggt tgcaggcttc cagttctgat
     1741 agttctccgc ttcgtattgt tcaaagaggc tcgctatggt aatgctaggc gtaacttctg
     1801 gttttacttc tggagcatta acagataggg acacagagtc tgcttggtta tcttgcctat
     1861 caatatcatt aaaaagatcg acgtaatcta tcagaggctg actgtctccc gcttcgatac
     1921 gcctcataca ggcttttagc accttcaggg cctcattgat gtaacgatgc tggtcaatgc
     1981 ttagcggctc actagccaga gcatcggtaa ggctctcacc aagttctccg tattggtccc
     2041 ggtagatatc cctcatgtca ggctcaaaga gatctgaacg gcccatagac agttcacact
     2101 cagcgatgtc cttaaggtgt tccctcattt cctcataggt agcgttgggt ctgtctgcgt
     2161 gtattgcctt gagtgctgcc tttatgtgcc ttgagtaagc catagcggtg cgtctatcct
     2221 tggtccgtaa tgatattgag gtcattctat cattgtttga ttgtctgacc ctaagacgga
     2281 agtagtagac gccgttacgc tggtagagat agtagactgc tggaagtttg tgactactgg
     2341 atagctgaat catatgtctg catgccctga gagggttaca cagtgggtta cagtaagtaa
     2401 ctctgcaagg aggcttaaag ccgtatccct taggattcag taggttggag tactttaaga
     2461 aaggtc
//
LOCUS       DAJVQR010000226         2462 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVQR010000226 DAJVQR010000000
VERSION     DAJVQR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..546)
                     /locus_tag="OQM52_004150"
     CDS             complement(<1..546)
                     /locus_tag="OQM52_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565761.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2197)
                     /locus_tag="OQM52_004151"
     CDS             complement(1208..2197)
                     /locus_tag="OQM52_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565762.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2281..>2462)
                     /locus_tag="OQM52_004152"
     CDS             complement(2281..>2462)
                     /locus_tag="OQM52_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565763.1"
                     /translation="REMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAA
                     IVADLESGGNWMDSL"
BASE COUNT          651 a          567 c          589 g          655 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gagttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagcctac gacaaatcga agacaaccag ctaacctatt gtttcttaaa gacaggtgaa
      841 tccagttggt tgtagtgata gtcacacatt tagagataga aggtcaagac ttgaagcacg
      901 taatattccc tctaaaggaa tagaactcac ggtctgacta cccggccctt ttggtctggc
      961 tcaggtcagg ctatgggggc ttttggctcg ctctttatat tagatgaggt ctcacatttt
     1021 tcagtcaaaa ctcatcgtct aatagcccat agccagacca ccagtcccag ctttagcagg
     1081 aagaccacga gatagacttt gtgtaggtcc atttggccct cctactttct gtattcacaa
     1141 actagattac tagtacaaaa taaaggccag acatccagtt cggaagtctg accaatagtc
     1201 gttattgtta gttagcttct ttagtgaagc tcaggcgttt gactacaggc aggacagcag
     1261 tagccgagcc agtacccatc ttgatggtcg tagcagcttc agctacaaca aacaccttgc
     1321 cgagaatctc aaggtcgtga gactgggagt ctccatcacg tgaaaggacc acagtaatgc
     1381 tctcgccatc gtgacggaag caaccgaagc tcagagccgt aaaacgctta gccatcatga
     1441 acacgccacg gtcttcaccg ttgtaatagg cgacgtcagt ccccagcata tcgacgatag
     1501 cgtcttcacc accgaagccc tgcttagcag ccagacggga gagagccttg aggtgtgact
     1561 catgggcgat aatgccgtag tcagaaacct cagaaccgaa agaagactcc gtgttctcgt
     1621 acagggcatc catgatagcg tcagccttag cggtcaggct accagtcagg gccggaagtt
     1681 ctacggtctt cagcagcttt gaggactcca gcacgtcaac catcttagag ttaatggtac
     1741 gaatcacgtt atcgcagatg ttcagtgcgt cctgctggag cgtcccctga gtgaaaccag
     1801 tggtcgactt ctggtcccaa agctttgttt ggtgagacac gaggatcgcc tgttcaatcg
     1861 gctcaacggt gatttcagtg ccagacggag cggtcacaac ttcaggtcgc ttaccaccac
     1921 ggacagggac cagcacagag cggtcagcca gagtgtgagc aatgattgga gtcggtaagc
     1981 tgttacgggt cggcaggact tctacaggga acgggatgtt atcttgagcg tctttaagta
     2041 caccgttcag catagcctta accgtcttag ctaactggag ggcatagacc tgagggtcgg
     2101 cgttagtcgg cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct
     2161 caatgccaaa ctggaaagcg tttttagtca tattcattat aattaatctc cttgagtcaa
     2221 atacttgaag tccctaacag gcagtcaggg accgtgtgtt ctcagatgag ggacgcttgc
     2281 ttacaagctg tccatccagt taccgcctga ctcaaggtca gcgacgatag cggcacgtga
     2341 gcgaccagcg gaatgagtag cgctgcggag gttttcacca taggtccggc gttcagcttt
     2401 gacctgttcg cggcgctgta gctctttttg ccattcgtct tcggtcatga acatttcgcg
     2461 gg
//
LOCUS       DAJVQR010000227         2441 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVQR010000227 DAJVQR010000000
VERSION     DAJVQR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..567
                     /locus_tag="OQM52_004153"
     CDS             <1..567
                     /locus_tag="OQM52_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1565764.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="OQM52_004154"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="OQM52_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS1565765.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="OQM52_004155"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="OQM52_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1565766.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="OQM52_004156"
     CDS             2169..2405
                     /locus_tag="OQM52_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1565767.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          534 c          627 g          652 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgaagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat a
//
LOCUS       DAJVQR010000228         2344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVQR010000228 DAJVQR010000000
VERSION     DAJVQR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            232..469
                     /locus_tag="OQM52_004157"
                     /pseudo
     CDS             232..469
                     /locus_tag="OQM52_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            890..1468
                     /locus_tag="OQM52_004158"
     CDS             890..1468
                     /locus_tag="OQM52_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565768.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1577..1835)
                     /locus_tag="OQM52_004159"
                     /pseudo
     CDS             complement(1577..1835)
                     /locus_tag="OQM52_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1819..2344
                     /locus_tag="OQM52_004160"
                     /pseudo
     CDS             1819..2344
                     /locus_tag="OQM52_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          745 a          423 c          583 g          593 t
ORIGIN      
        1 gttatgatgc atgagccgct ggctcgaata cggtggtgcg gctgagagtc gcagagggga
       61 agttgcgtcc atgttgaggg gaacggagca aaaaaccatc agagatacat tgaatcaatc
      121 aatgtaagca atattaagtg tctataccga ttacttgatg atcaggttgt gttgaataaa
      181 cgcgcattct tcggtgcttt tttaatcttt tggggattta aattcctatc gatgaactgt
      241 cgatatgaaa aagcgagcat catactgacg tcaaacaaag gggtcgcaga ctggggagaa
      301 atgttcggag atcacgtgct ggcaacagcg atcctagatc ggctgctaca cgctgaatat
      361 caaaggagag agttaccggt taaaagagaa acgtaaagct ggagtgctga ccaaaaacac
      421 aacgccaatc agtgatgatg aaatggtgga aaacggacag catcagtaac gaaagtatct
      481 tagcaggcat gaaaatcgtc cgcatcccct aaacggtcaa aatgcttctg ccgtgaagga
      541 aggagaatgc catctcatca cttatatctg gcgaatgaaa cacagatggt cggccttgcc
      601 cccaaacaca cggcggtgca aaatggaatg gcagaaaact tcgtcaaaac gatgaaatat
      661 gattaaatca gtgttatgtt cgaacctgac gtgttaacag cagtaaagta gtctcgcgaa
      721 ggtgttcaaa cattataatg aatggcatcc tcatagtgcg cagggttatc gcttgctgcg
      781 gaaatatctg aagcgacgta ccagtaatgg ggaaaatgat agctggtttc tgaaaatata
      841 gaggaaaatg tgatatatat atcctgttaa tatttaagga gaatataaaa tgaaagtctc
      901 gtttaagtct ttaggctata tttttcatga tatatataat aaaaaacaca ctatcgatga
      961 gttcaacgat gtggtaagaa aagcagtttt atcaggtaaa attaacgagc tgaatgcatg
     1021 tcataaagtg gcaatttttc ttgccgaaaa agacaatgag ataacaaaga aagacaaagc
     1081 caagataata gatacattaa ctgaaaacta ttccattgaa tttcaacaat taatgaatat
     1141 tagtgagcgc acgttaaatt catctttata tattactcct ggtgaaagtg gttttgtcag
     1201 ttttgttaat cgtgaaggaa aaatatgtca tactgcatat gtaaaatcat cagataatag
     1261 catgacatat taccatgcaa atggtagcag cattgacaaa tatattacgg atatgtgcgg
     1321 ccttatttgt atgaggcata ttgagtctac tggcattatt ttttatatgc ttgatgaaaa
     1381 agttttatct gccattgctg aatttatgaa tgaaaaagga tggcgggctg cgttttgttc
     1441 agcaaagaac ttatataaat gtgtttaatt ttagaaaaac tatttggtaa aatccaacaa
     1501 gagtcgagag gttcgcgttg cttcagcagg gcctcagcct gttcaaccag actcagttgc
     1561 aggtcgtgac gcagatttag tggtggtggt tgattgtcag ataccagccg atgatcttgt
     1621 agtgcatatc atcagacttt gagaagcata tggttttgcg ggtaagccgc ttaagatgtg
     1681 tccgcaggtt cagattatgt cgctctatcc gctgagtgca tttcttgctt ataacatgac
     1741 tggttgagga caataaagtt cggtataccg gctcaggcat cggtcatgta gaaggcaatg
     1801 ctaaactggc tcagcaaact aagagcagcg cgcatccact tacccaatgc ggttgagaaa
     1861 atagccttcg atcgctttca tgtggcgaaa caaccgggtg aggtggtgga taaaacccgc
     1921 cagaatgaac atccgcatct tcctgttgaa agtcgtcgtc aggcaaaggg aactcgcttc
     1981 ctgtggcagc acagcgataa atggatgacc gaatccaggc aggaaaagct gatatggctg
     2041 cgtgcgcaga tgaagctgac gagcctgtgc tgggcgctga aagaactggc aaaggatatc
     2101 tggagcagac catggagcga ggaaagacgc aacgactggc agagatggct gcggccaaca
     2161 gtgacgtccc catgatgaag aatgtggcta aaacgatagg gaaaaggttg tacggtatcc
     2221 tgaacgccat gcgccatggc gtatcaaacg gaaatgcgga agcactgaac agcaagatca
     2281 ggctgctgag aataaaagcc aagggctacc gcaaccggga gcgctttaag ctgggagtga
     2341 tgtt
//
LOCUS       DAJVQR010000229         2331 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVQR010000229 DAJVQR010000000
VERSION     DAJVQR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..787)
                     /locus_tag="OQM52_004161"
     CDS             complement(<1..787)
                     /locus_tag="OQM52_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1565769.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSL"
     gene            complement(1387..1635)
                     /locus_tag="OQM52_004162"
     CDS             complement(1387..1635)
                     /locus_tag="OQM52_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1565770.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1990..>2331)
                     /locus_tag="OQM52_004163"
     CDS             complement(1990..>2331)
                     /locus_tag="OQM52_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1565771.1"
                     /translation="AKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRR
                     GIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIAR
                     VRSVEEILHAL"
BASE COUNT          605 a          430 c          557 g          739 t
ORIGIN      
        1 gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt gaacattcat
       61 tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca aaataaccgt
      121 tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt gcccatatgg
      181 agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt gcgctaatct
      241 cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat acatctaatg
      301 tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag acatgaagct
      361 ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac gcattcaata
      421 atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg acattaagtt
      481 gtgtaaggga cgcaggcaat ggggagatta agcttaaatt atttttactt atattaattg
      541 atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac aagtccaggt
      601 tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct cgttgttctt
      661 cttgtatgcg gttatttttc cattcagtcc aatgggttag ataattttca tatgcgcggc
      721 tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg ttatttgtcg
      781 ggagcataaa tatcagggtg tattctttat gttgagaggt gttggattct tttttatctg
      841 ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg aggcggatat
      901 atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc tgctttacac
      961 tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg ggggcaattt
     1021 ttccttaagg tatgaaaaaa ggagctgaag ctatataata gctattaatg ccactggtat
     1081 cagtgaatca tgtatataaa aaactgtgtt aaggaggctg tccattagcg gttttttatt
     1141 cttctggaac cgcggcatct ttgcgtatct ttttgtcttc tcaccgtctg gtcggtgttt
     1201 tgctgagact gctaatttcc tgtctttgct agtgctgtac tggtactgcc caatcatgat
     1261 tggcgggggc gggcggagtt gagaccgcag acacgtcgta tgcaagaacg tgctgcggct
     1321 ggctggtgaa cttccgatag tgcgagtatt gaatgatttc cagccgttac tgattttacg
     1381 tgctaattag tgaacaaacc actcgtcagc agactcccag gtatctttca gagtttcctg
     1441 aacaaaagtt ttagctgaat ctttatcggc ggtgcgcgta acagaaaggc catcgttgct
     1501 ggtggctttt acgatcacct ctacatcgtc ataacgttta ctgatgcgtc gggttaattc
     1561 ttcctttaac gcatccacag caccggttgg cattttagtc attttctctt tggctatgca
     1621 gatttcaaaa cgcataaaaa tccctctgca ctgtgttttt atacagtatt atttttaact
     1681 gtatggataa acagtgtcaa gagtgaggga aagtgatttt ttggggcatg catggggcaa
     1741 aaatatttgt tgtggggcat tttggggcat gattgggaca ctttgttaca tatgaacttt
     1801 gccgattttc atatgacatg gcaatccaac gctttgatta ttctaaagaa aacacatgct
     1861 cttgggcgtt ctttagtgat ttttaaaaat cccgtatcac gcagttaaag tggcgggcat
     1921 actcttcaag gctggtgatc cccaaccgca cccatttagg atgtgaccat tgcggtagac
     1981 cgatgtaaat cataacgcgt gaaggatctc ttccacgcta cgcacacggg cgatgcgcgg
     2041 gtagatatga ttaatgctgt tattgtgttg ctcggcgctg gcggcactac aggcgtcttc
     2101 ggcaatcacc agattaaagc caagttccca ggcattgcgg gcggtggatt caacaccgat
     2161 attggtcgag atcccacata acactattgt atcgatacct cggcggcgca gttgcaactc
     2221 cagatccgta ccgtaaaacg caccccactg acgtttgatg atttcgatat cactgtcagt
     2281 tgcacctaat gcagctggat gttgccacca gttttcgggc aacactttag c
//
LOCUS       DAJVQR010000230         2268 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVQR010000230 DAJVQR010000000
VERSION     DAJVQR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OQM52_004164"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OQM52_004164"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1565772.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          576 a          561 c          668 g          463 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactcta
//
LOCUS       DAJVQR010000231         2233 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVQR010000231 DAJVQR010000000
VERSION     DAJVQR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            453..1625
                     /locus_tag="OQM52_004165"
     CDS             453..1625
                     /locus_tag="OQM52_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1565773.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(1679..>2233)
                     /locus_tag="OQM52_004166"
     CDS             complement(1679..>2233)
                     /locus_tag="OQM52_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565774.1"
                     /translation="TRPVTVVINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTT
                     FQSAGDYQPSAERPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSI
                     DVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFR
                     VTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          702 a          448 c          399 g          684 t
ORIGIN      
        1 ctaatcacct taccgaacac gcacggcaaa tagactcgat cctgaaccca tcggtcccaa
       61 attcgtccca gtcaaaaaat aaggaaggta ctaatgatgt gtaacttatt gatttaaatg
      121 gtgccgataa taggagtcga acctacgacc ttcgcattat gaatctgtag caccaatcat
      181 aactatctgt tttagcaagc attaaccgca ttcactaagc tatagttgat gacacaaaca
      241 gaaagttgat gcatgatgtt gccatatata tgtcacaaat acggcacaac gatcttcaaa
      301 catatagcca cacattcaca gaagagcaca aagccttgca atccagtgca aagcttggtg
      361 tgtctccgtt ttgtttcatc agttacacat aactatatga tcattaacta aaagtcagta
      421 tacttggaga aattttaatt gcgtaataga gcatgcatat gattgaaatt aacagtttat
      481 tattaataac atccgtgata ttgatgtcgt tattagctgt ggggttattt gataaaattt
      541 ccccaataaa tcttgttgag cacggcagga ataatcaaat agatggaatg cgggggttct
      601 tagcaatttt cgtgcttatt catcacgcag caatttggaa tggatacttg tcatctggag
      661 tatgggaagc accttcatca aatctgttag caaatttagg ccaagttggt gtgtcattct
      721 tttttatgat tactggttat ctgttctttt caaagattat ctctggagat caggactgga
      781 caaggcttta tgtatcaaga ttactacgat taaccccaat gttcatagtt agcctatgtc
      841 tcatttttat cattgtaggt tttaagtctg gatggagaat gcaggtatcc acagaagagc
      901 tttttgtgtc aataatgaag tggttgccat tcactgcgct aggtatgccg aacattaatg
      961 acgtaaaaga ttcatttact atcaatgccg ctgtaacatg gacacttgta tatgaatggt
     1021 tcttttattt ttctcttccg gtaatttccg cgctcataaa aagaaaagtc agtatttaca
     1081 tggttatgat tagcgcaata tcgctatttg ttttcatttt atttttcagt aaaatacaca
     1141 tagcatcatt tctttttggg ctcctggcat ttttgctaaa taaatcaaag atagtcaatg
     1201 gaattgccaa agcaaaagta acaccaatta taataactgc gataatggtt tttgaaatga
     1261 cgtatttcaa aacaacttac gcaccgctac cactgattct ttgtggaata acatttatca
     1321 ttatcgcatc aggttgtgat ttgtatggaa tattaagatt aaatataacc agaaagctag
     1381 gagaaactac ctacagcgtt taccttctgc atgggatatt cctttattgc ctaatgacgt
     1441 ggattattcc taataattac actgaaaata catttatcat actggtaagc acaactgcat
     1501 ttcttattac gttcacatca tgcctaactt ttaaattaat agagacgcca ttcatcaaat
     1561 taacaaaaca aaccacaact ttagtaaaag aattaatacc cacattaaca aacaacaatc
     1621 aataaacaat aaacataagc cgccctacat atatgtccaa atggggcggc gaatgtattt
     1681 atattagtct ggtaatgcta aatgcaaacg aactggagat tcctgccccg ctaagcgtat
     1741 tgcgcagggt caccctaaat gtatctacgc caatataagt aacatcacaa ctaactgaat
     1801 caggcgcaga tataggtgtt atctgcacaa tataaccgct agcaactgct gcgagcggat
     1861 gagaaaagct aacatcatac acacctgtag aaaccacgtt aaccgatgaa acaccagtag
     1921 caacgtcgat actaccattg gcgttaattt ttcccgagcg tgtgactgac aatgcagaag
     1981 ccgatgttac cttactggtt gtttctgtaa aaacacaccc aatgtcaact acctcgcaat
     2041 tagctcctgt tatccagtat ggtcgttctg cacttggctg ataatcccct gcgctctgga
     2101 acgttgtgcc gttgagtatg actgtaactt ttcccccacc tgagcttgtt acttgaatat
     2161 tcgtgtgcgt gtatcttgca ctgctcacgc gatgaaaatt tccaccgttt ataaccacag
     2221 ttacagggcg cgt
//
LOCUS       DAJVQR010000232         2229 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVQR010000232 DAJVQR010000000
VERSION     DAJVQR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(121..546)
                     /gene="arsC"
                     /locus_tag="OQM52_004167"
     CDS             complement(121..546)
                     /gene="arsC"
                     /locus_tag="OQM52_004167"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1565775.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OQM52_004168"
     CDS             complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OQM52_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1565776.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1902..>2229)
                     /gene="arsR"
                     /locus_tag="OQM52_004169"
     CDS             complement(1902..>2229)
                     /gene="arsR"
                     /locus_tag="OQM52_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1565777.1"
                     /translation="FKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRHLAL
                     LRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNCSGD
                     SKNICS"
BASE COUNT          592 a          615 c          556 g          466 t
ORIGIN      
        1 agtaagttgg cagcattacc tgaatttgca cggttttgta ggtcggataa gacgcgttag
       61 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      121 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      181 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      241 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      301 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      361 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      421 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      481 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      541 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      601 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      661 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
      721 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
      781 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      841 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      901 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      961 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
     1021 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1081 tgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
     1141 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1201 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1261 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1321 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     1381 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1441 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1501 aagcgccgcg ccatcattgg caaacagggc cgcaacggcg gcaccgagca ggacaatcca
     1561 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
     1621 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     1681 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
     1741 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1801 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     1861 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
     1921 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
     1981 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
     2041 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2101 gccagatggc gggatatctt gggctgcgac tgatcgaggg cagtgcagag atcgcagacg
     2161 cataactctc ccagttcgct aagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2221 attttgaac
//
LOCUS       DAJVQR010000233         2210 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVQR010000233 DAJVQR010000000
VERSION     DAJVQR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(69..224)
                     /locus_tag="OQM52_004170"
     CDS             complement(69..224)
                     /locus_tag="OQM52_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1565778.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(427..834)
                     /locus_tag="OQM52_004171"
     CDS             complement(427..834)
                     /locus_tag="OQM52_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1565779.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            911..1138
                     /locus_tag="OQM52_004172"
     CDS             911..1138
                     /locus_tag="OQM52_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1565780.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1122..1544
                     /locus_tag="OQM52_004173"
     CDS             1122..1544
                     /locus_tag="OQM52_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565781.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1622..>2210
                     /locus_tag="OQM52_004174"
     CDS             1622..>2210
                     /locus_tag="OQM52_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565782.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMSGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPVEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTE"
BASE COUNT          580 a          455 c          579 g          596 t
ORIGIN      
        1 gttgctggag gcgttttgca tcttctagga gtagagcgat aacgtgtttt aattctgtct
       61 cgttcatttt actcacctga atgtcttccc aaccaacgac gtgcgccagc ttcggtttta
      121 aacgttttgc ttttggtata cgttatggcg gtgaatgtgc cgtcctggtt ggggaacacg
      181 ccgtacaccg gagattcgtt gttgccaaga tcgatagtat tcatgttgac cccatttccc
      241 cttaacgccg gggtagcgga actgtttgct gagaacaccg tgcggtgtct tgatgaataa
      301 aatttagaat aacctaatga gggtgatcaa gatttttgtg tagaaaaacc taagtttttt
      361 gatgtaaaaa acacaagtgt ttgaaagttt gtgcttttta ttacagggtg tggagaaaaa
      421 aggggattat ttgtttgcgc ttcttttgcg agctttgagt agttcttcaa aaagtttgtt
      481 gaaattttca actcgagcac gcatctctga caacagggcc ttttgctctg actcaggcag
      541 tgcgtcgaac agttgaagta actctttttg atcttctgtc agaatggctg gctgattatc
      601 cgggatcggt tcgcctggtt gtttatcttc atccccaaaa agaagccaag tcggtgagca
      661 ctgaagcgct tggctcagtg cgaataatct tttccccgcc ggctgtgttt catctctttc
      721 ccattgagaa attgttacgt gagccacttt gaccagctta cctaatgcgg cctgagacag
      781 ttttaatttt ttacgcctat gtaagaggcg agcaccgaag gtttcgtttt tcatattagg
      841 gaattctaat ttttcttgac ttaggtttct ctacggcctg gtttccttag gaaaatctaa
      901 ggagctcgat atgttgaaaa ttgatgctat agcgtttttt ggcagcaaaa caaagcttgc
      961 caatgccgca ggagttaggc tggcaagcat tgctgcatgg ggggaactgg ttcctgaagg
     1021 tcgcgcgatg cgcctgcaag aggcatctgg cggggaactt cagtacgacc ccaaagttta
     1081 tgacgaatat cgtaaggcaa aacgacctgg gaaggtgatt catgaaaatc aggcatgagc
     1141 acatcgaatc agtgctgtta gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1201 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1261 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1321 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1381 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1441 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1501 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1561 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1621 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1681 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1741 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1801 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1861 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1921 aaaaagctct aaaaaacggg cggttacaaa cgagcgagta acaaaaatac gcgaactgaa
     1981 acgaaaaggt aacgctgcca gcgttacaca aacggatcaa aaagcgttac cagtggaaga
     2041 ggaagaggaa gatctaaata ctgatctccc cctaaatccc cctcgccaaa aacgagcgtc
     2101 taaaaaattc gagccggagg ctattgagct gcccgattgg ttgccggaaa cactctggca
     2161 tgagtgggtc cggttcagac aggcattgcg aaaaccgatt cgaacggagc
//
LOCUS       DAJVQR010000234         2179 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVQR010000234 DAJVQR010000000
VERSION     DAJVQR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..363)
                     /locus_tag="OQM52_004175"
     CDS             complement(<1..363)
                     /locus_tag="OQM52_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1565783.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="OQM52_004176"
     CDS             complement(430..1644)
                     /locus_tag="OQM52_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCS1565784.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          658 a          535 c          447 g          539 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcta ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttgaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggtcacca gaatgctgta
     2041 atgccagtca gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc
     2101 ctctccttta ccactctcgg gaaggccctt ccccttctcg tcggtaattt cacaagttgt
     2161 cccatacttg caagatcgc
//
LOCUS       DAJVQR010000235         2122 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVQR010000235 DAJVQR010000000
VERSION     DAJVQR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..799
                     /locus_tag="OQM52_004177"
                     /pseudo
     CDS             1..799
                     /locus_tag="OQM52_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OQM52_004178"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OQM52_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="OQM52_004179"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="OQM52_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          523 a          512 c          642 g          445 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc ctggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc ttaggggggt
     2101 gcccctatta agttgtcaag ca
//
LOCUS       DAJVQR010000236         2120 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVQR010000236 DAJVQR010000000
VERSION     DAJVQR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(220..384)
                     /locus_tag="OQM52_004180"
     CDS             complement(220..384)
                     /locus_tag="OQM52_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565785.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="OQM52_004181"
     CDS             1286..1570
                     /locus_tag="OQM52_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1565786.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="OQM52_004182"
     CDS             1558..2043
                     /locus_tag="OQM52_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1565787.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          548 a          445 c          605 g          522 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc
//
LOCUS       DAJVQR010000237         2118 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVQR010000237 DAJVQR010000000
VERSION     DAJVQR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            277..581
                     /locus_tag="OQM52_004183"
                     /pseudo
     CDS             277..581
                     /locus_tag="OQM52_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            750..2114
                     /locus_tag="OQM52_004184"
     CDS             750..2114
                     /locus_tag="OQM52_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1565788.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          548 a          417 c          489 g          664 t
ORIGIN      
        1 ggggggttgg ggagcaatgg aacagtaaac gccgttaaga agtcaattta aatagccaaa
       61 aattaactag ccaatttaca ttgcaggttg taatttttaa ccaccttttt tacgatagcc
      121 aaattaccta tacccatccc ggccctgcca agccataccc gctatcggcg atgaaaatgc
      181 ttttgtgcgc ggtcggagtg gtcccgacga gggttttacc cgaagtcggg gcgtatctcc
      241 gcgttagcgg gccgtgaggg ccgcttacga gcgtgtatga gagacgtaca gtggttttgt
      301 ccgctcacac cagtacatac aggtacggaa ataaaaccag tcgaaatgcc ggtaacgacg
      361 attactcctg tctctgatgc cggtggacta caagatttta tttactggcg tcctgatgcg
      421 gacagggctg gtatggaacc tgtttatgtg atacttaatg atcctctgga ttccgggcga
      481 tttacgcgta aacaactaaa taaaaatatc ttaaacatgc aagtaatttt ggtattctcg
      541 atacaagaat aaatagtagt aactactata gctgcaaata atgttaaaaa catttctatg
      601 atcatcttta catttttcat gattgacaat tgtttttggt taaaaaaaga caactaatga
      661 gtgtgataat gcaatacttt gagtgattat ataatgataa gtaaacaggt ttgcgacaat
      721 tggttatcag tattacagaa ggattaaata tgtcattatt cacagaacct aaggaaattg
      781 aaagattacc ctctgaagag attgaacgat tatatcccgt cctgcgatac agggtcttta
      841 tcagcatttt cctcggatat atggggtatt attttgtccg aaacaccacc agtgtgttat
      901 ccggtgtgct gcacatgtct gcaaccgaga tcggcattat ctcctgtgcc ggttttttat
      961 cctatggtat atcaaagttt gtttccggac ttatttctga cagaagtaat tcaaaagtat
     1021 ttttgtctct tggtttattc ctgtccggac tggtaaattt tctgatagga tatattcccg
     1081 ggatcatcac atccgttacg ttattttcaa caatgtattt acttaacggc tggatccaag
     1141 gtatgggata tcctcctggt gccaaaacac tggtgttctg gtatgaacat cgggaaagga
     1201 tcacctgggc cacactgtgg aatttatcgc acaatgtcgg gggagctctg gcccccgttc
     1261 tgattggttt cagttttggt tttttcggcg acagtgctct ggatcatgcc cgggccgcat
     1321 ttatcttccc aggagtactg tgtatggcaa tgagtgtgct gatttacttt attcaggtgg
     1381 acagacctgt atcagtgggg cttcctccca ttgaagaatg gaaaggtaac gtcgtttcac
     1441 acccggctaa aggccgtgaa caaggtccga gactctccat tcctgatatc atcagaaaac
     1501 atatcatccg aaataataaa ctcatttact gctgtatcta tggttcgttt gtttatattc
     1561 tgagatacgg tattgtttcc tgggctccta agtttttaag tgacagtctg gatgtcggtg
     1621 gtaaggatat gggaaaactg gcatcaatgg gggggggttc tgtttttgaa atcggtggtg
     1681 tggctggtat gcttcttgcc ggatacctgt ctgtcagact gttcagaaac tcaaaaccat
     1741 taacaaatac tttatttctg gcacttacca ttattttatt aatcgcgtac tggtacgtgc
     1801 catcaggcaa tgaatatctc tggctaaact atacaatcct tattctgttg gggctggcag
     1861 tgtatggtcc ggtgatgttt attggtcttt attcaatgga gcttgttcct aaagaagctg
     1921 ccggagccgc atcagggctg tcaggcacat tttcctatat cttcggctca attgttgcga
     1981 cactgggaat gggacttgtt gtcgattatc tgggatgggg ggccacattt atcgtactga
     2041 tactgtctgc tgtttttgcc atcattttca cactgatgag tcgtgaacgc tcactggagt
     2101 tcgaaaaaga gtaatttt
//
LOCUS       DAJVQR010000238         2092 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVQR010000238 DAJVQR010000000
VERSION     DAJVQR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OQM52_004185"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OQM52_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1565789.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="OQM52_004186"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="OQM52_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS1565790.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAJVQR010000239         2051 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVQR010000239 DAJVQR010000000
VERSION     DAJVQR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OQM52_004187"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OQM52_004187"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1565791.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2051
                     /locus_tag="OQM52_004188"
     CDS             1647..>2051
                     /locus_tag="OQM52_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1565792.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGM"
BASE COUNT          556 a          430 c          525 g          540 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat g
//
LOCUS       DAJVQR010000240         2031 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVQR010000240 DAJVQR010000000
VERSION     DAJVQR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..246
                     /gene="lsrG"
                     /locus_tag="OQM52_004189"
     CDS             <1..246
                     /gene="lsrG"
                     /locus_tag="OQM52_004189"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCS1565793.1"
                     /translation="GEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFYIYEAYKDE
                     DTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            286..1044
                     /gene="tam"
                     /locus_tag="OQM52_004190"
     CDS             286..1044
                     /gene="tam"
                     /locus_tag="OQM52_004190"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCS1565794.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1048..1962)
                     /locus_tag="OQM52_004191"
     CDS             complement(1048..1962)
                     /locus_tag="OQM52_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCS1565795.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          539 a          491 c          525 g          476 t
ORIGIN      
        1 ggcgagttta tcgaagtttt tcgccagaac cacctgggct ctgtacagga agaaggtaat
       61 ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc gcttttatat ttacgaagcc
      121 tataaagatg aagacacagt ggcattccat aaaaccacgc ctcactacaa aacctgtgtc
      181 gcgaaactgg aatctttaat gaccgggccg cgtaaaaaac gtctgttcaa tgggttgatg
      241 ccgtgaggct acaattgggg taacgcgctg acgggagtaa aaaaaatgtc tgactggaac
      301 ccctctttat atctgcactt ttccgctgaa cgatcgcgtc cggcggtgga gctgcttgcc
      361 agagtgccgc tggagaatgt cgaatatgtt gccgatcttg gctgtggccc aggtaacagc
      421 accgccctgc tgcatcaacg ttggcctgcg gccaggataa caggcatcga ctcgtctccg
      481 gcgatgattg ctgaagcgcg cagtgctttg ccagactgcc agttcgtgga agcagacatc
      541 cgcaactggc aaccggaaca ggcactcgat ctgatttttg ctaatgcctc actgcaatgg
      601 ctgcccgacc actacgaatt gtttcctcac ttggtttctt tacttaatcc gcagggcgtg
      661 ctggcagtac agatgccaga taactggctg gagccgaccc atgtgctcat gcgcgaagtt
      721 gcttgggaac aaaactaccc ggatcgcggg cgtgagccgt tggctggcgt tcatgcttac
      781 tacgatattt tgagcgaagc cggatgtgag gtcgatatct ggcgaaccac ttactatcac
      841 cagatgccgt cgcaccaggc gattatcgat tgggtgaccg ccactggatt acgtccgtgg
      901 ttacaggatc tgaccgagag cgaacagcag ctttttctta cgcgctacca tcagatgctg
      961 gaagagcagt atccactgca agagaacgga cagatactgc tggcatttcc gcgtctgttt
     1021 attgttgccc gccgtacgga gtaattatca cgtcagctgg taatgacgat cgggaagaat
     1081 ttttgctgga atttctgctt catcgttcat ctgtaacagg tcaatttcaa tagcgttgca
     1141 gatggcatcc agcggtaaat cattgttttc agtaccgaac ggatcttcca gttcttccgc
     1201 cagacaatcc agcgaaataa aagtgtagga aatcagcaca gagataaaag gcgtcatgta
     1261 atgcaggtcc acgaccagcg cgaacggcag catgatacaa aacagataaa cggtacgatg
     1321 caaaatcagg gtgtaggcaa agggaattgg cgtgtaggca atgcgctcac agcctgccag
     1381 aaccgctgaa atatcattaa gccgatcgtt gaggctaata aacaggatat ctgaaagctg
     1441 tccattgcgg cgctgaaccg ccaaccattc tcccattatt aacaagatac ggttagctgg
     1501 agagttcgaa gccagtacac gctgaagatc tccagtcttg agataatgag ccagcacttc
     1561 cgcctgtggc tgtttgcgta atgtcatgcg taaacagtgg gcgaaggcga tttgcagcct
     1621 ggcaaactcc cttacacttg ccgaatccgg caatgtcgtt tttacctctc gcagtaacga
     1681 ccgtgaggca atcatcaact gcccccaaag ttttcgcgct tcaacgtaac gggcgtaccc
     1741 ggcattatta cgaaaaccaa gaaaaatggc gatggcgaca ccgagaatgc tgaacggtgc
     1801 gagggtgaat ttgatgccca gatgcgtgta ccagggcagc atgaatataa cagcaataga
     1861 aaagagaaaa ttgagtagta agcgcgagga tatcttggat aatactgagc cgtgccagac
     1921 aaaaatacgg cgcagccagt gttgttgtgg acgaacaatc atggttatct tcaggcgtgg
     1981 aaaagtcgcc ctattaaacg tgattacgat cacattctca agacgctact t
//
LOCUS       DAJVQR010000241         1980 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVQR010000241 DAJVQR010000000
VERSION     DAJVQR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>1980
                     /locus_tag="OQM52_004192"
     CDS             <1..>1980
                     /locus_tag="OQM52_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1565796.1"
                     /translation="RPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMT
                     FIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIA
                     LYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPD
                     YQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQA
                     ERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFW
                     EQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKET
                     LAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSG
                     SGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTI
                     NDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADAL
                     AADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNAS
                     VTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSV
                     SSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEV"
BASE COUNT          569 a          472 c          471 g          468 t
ORIGIN      
        1 cgccctttcc tgctggctaa tacccgtaat ttagaccgtg aacctatcgg cctgatggat
       61 actgtcgcca cggtcacgcc ggcacaattg cgccaatttt atcaacgctg gtatcaacca
      121 aataatatga cctttatcgt ggtcggcgat atcgacagta aagaagcgct ggcgctgata
      181 aaggataatt taagtaagct tccggctaac aaagcagctg aaaatcgcgt ctggccgaca
      241 aaagccgaaa accacctgcg ctttaatatc attaatgata aagaaaaccg ggtgaacggc
      301 atcgcactct attatcgcct gccaatggta caagtgaacg atgagcaaag ctttgtcgaa
      361 caagctgaat ggagcatgtt agttcagctg ttcaatcaac gtctgcagga acgcatacag
      421 tcgggcgagt tgaagactat ttctggcggc actgcgcgca gcgttaaaat tgcacccgat
      481 tatcagtcgc tgtttttccg tgtaaatgca cgagacgata atatgcagga tgctgcgaat
      541 gcattaatgg cagagttggc aaccattgat cagcatggtt tttctgctga agaactcgat
      601 gatgtcaaat ctacccgcct cacctggctg aaaaatgcgg ttgatcagca agctgaacgt
      661 gatttacgta tgctgaccag tcgcctggca tccagctcat taaataatac gccgttcttg
      721 tcgccggaag agacatatca actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt
      781 ctggcggaaa aatggcagca gttaagaaag aaccaggacg cattttggga gcaaatggta
      841 aacaatgagc ttgccgccaa aaaagcattg tctcctgcgg ctatcctggc gctggaaaaa
      901 gagtacgcca acaaaaagct ggcggcttac atcttcccag gcagaaattt atcgttaaca
      961 gtagacgctg acccacaggc ggaaattagc agcaaagaaa cgctggctga gaatctgaca
     1021 tcattaacac tttccaatgg tgccaaggtt attctggcaa aatccgcggg tgaagagcaa
     1081 aagctacaaa ttattgccgt atcgaataaa ggcgatttaa gtttccctgc gcagcaaaaa
     1141 tcacttatcg cgctggcaaa taaagcagtt agcggaagcg gcgttggcga actctcctct
     1201 tccagcctga aacgctggag tgcggaaaac tcggtaacca tgagcagtaa agtcagtggc
     1261 atgaatacgt tgctctccgt tagcgcgcgg actaataacc ctgaacctgg tttccagttg
     1321 attaaccagc gaatcaccca cagcacgatt aacgacaata tttgggcatc gctacaaaat
     1381 gctcaaattc aggcgttgaa aacgctcgac cagcgtccag cggagaaatt cgcccagctg
     1441 atgtatgaga cgcgctatgc tgatgaccgc acgaaattac tgcaagaaaa tcagattgca
     1501 cagtttactg ccgcagatgc gctggctgcc gatcgccagt tgttttcatc tccagcggat
     1561 atcacgtttg tcattgtcgg taatgtcgca gaagacaaac tcgtggcgtt aattacgcgt
     1621 tacttaggct caatcaaaca ctctgattcg ccattagccg caggtaaacc attaactcgc
     1681 gcgacggaca acgcatcggt tactgtaaaa gaacaaaatg aacctgtggc acaggtttca
     1741 cagtggaagc gttatgattc ccggacacct gttaatctgg cgacgcgtat ggcgctcgat
     1801 gcttttaacg tcgcactggc aaaagatcta cgcgttaata ttcgtgaaca ggcatctggg
     1861 gcatacagcg tttcttctcg cctctcggtt gatcctcagg ctaaagatat cagtcatttg
     1921 ctggctttta cttgtcaacc agaacgacat gatgaacagt taacgttagc gaatgaagtg
//
LOCUS       DAJVQR010000242         1914 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVQR010000242 DAJVQR010000000
VERSION     DAJVQR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(53..1702)
                     /locus_tag="OQM52_004193"
     CDS             complement(53..1702)
                     /locus_tag="OQM52_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1565797.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          507 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aatc
//
LOCUS       DAJVQR010000243         1913 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVQR010000243 DAJVQR010000000
VERSION     DAJVQR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(854..1327)
                     /locus_tag="OQM52_004194"
                     /pseudo
     CDS             complement(854..1327)
                     /locus_tag="OQM52_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1255..1621)
                     /locus_tag="OQM52_004195"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="OQM52_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1913)
                     /locus_tag="OQM52_004196"
                     /pseudo
     CDS             complement(1623..1913)
                     /locus_tag="OQM52_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          536 c          459 g          493 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgcggacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tga
//
LOCUS       DAJVQR010000244         1902 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVQR010000244 DAJVQR010000000
VERSION     DAJVQR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..697
                     /locus_tag="OQM52_004197"
                     /pseudo
     CDS             1..697
                     /locus_tag="OQM52_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            699..1304
                     /locus_tag="OQM52_004198"
     CDS             699..1304
                     /locus_tag="OQM52_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1565798.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1291..1839
                     /locus_tag="OQM52_004199"
                     /pseudo
     CDS             1291..1839
                     /locus_tag="OQM52_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          469 a          451 c          524 g          458 t
ORIGIN      
        1 acgcccgtgc cggtgtgccg ggcagcgatg aggaaacccg tgcggacttt gaaaaccgcc
       61 gacgtaattc agttgcccgt aatgcccgga atattctgga agccatccgg ggtgaaatac
      121 tctccacggt agaaaacgtg gtggatgttt acgtcaccca taatccgaaa aaaacagaac
      181 aaaaagccgg agtcagtatc cattaacacc cggttcgctt tatgttggcg tgtacggcgg
      241 cagcccggca gatatcgcgg cggccatctg gcgtaaggct ccgccgggta ttgatatgaa
      301 tggcaacaca acgttcactg ttgcagataa ggagtacgat ccgccgtatc ctgaatacgt
      361 gatcagctgg cagacactca aacccgtcag tctgcatgtc agtgtgacgc tgaaaaaaag
      421 tgactacctg ccttcagata ttacccgaca ggtacagcaa tccgtgctgg acgcatttaa
      481 tggtacagat ggtggtctgc gggcaagagt tgcctctgtt gtctctgccg ggcgctacta
      541 tgccggcatt tacaaaaccg atccggaaca tattgatatt ctgggcctta ctgtgagccg
      601 tgatggttcg tcatggacaa cggctgtcac tttcgggata gatgagattc cggttctgga
      661 tgtgtcggat atcagtgtga aactccagga ggcataacgt gcagaatgtg gctgccactg
      721 tgcttgcgca gtatgctgcc agcccccgac tcaatgccct cattaacagc tttaacgcag
      781 cgctttcccc cgacagtttt atcagtgatt tttatggtct tatctggaac atcgataccg
      841 cagaaaagta tggtcttgat gtctggggaa agattgtggg tgtcagtcgc tggctgacgg
      901 taaaggacga ttttaattac ctgggcttca gcgagtccag gatggacacc ccggtaatgg
      961 atgatccctg tccgtttaat caggcaccgt tttacaacgg aaaatcggat acccggactg
     1021 ttgacctgtc tgatgctgta taccggcggc tgatactgat gaaagccatg tcgaacatta
     1081 ctgactgttc cgttccggat attaacctga tgctgagatt tatgttcgga aaaaaacgcc
     1141 gggcttatgt tctgaataat ggcgggctga ggatgagtta cgtctttgag tccgcgctct
     1201 cgttggcaga actggcgatt atccagtcgt cgggtgcact gccatccccg ccgggtgttt
     1261 atgtttcagt agttttaaag gagtcccgta atgaaggcca gtgataaacc ccgccagctg
     1321 gcggtcccct ttgcgagtac cggagataaa aaccgtatcc cggacaaggc gacacagcag
     1381 accagagaga gcggtaatgc tgcgtatgat tcaggttttc ctccggtgac catgacagcg
     1441 gtctcagcgg gaggtatacc gccacacggc aaggatttta acggtctgat gtacgatatt
     1501 accgcagcaa tacggttcgc ccaggctggc ggtttgtaca cgtataatgc cggttttgcg
     1561 ggggccattg gtggatatgc aaaaggagcc attctcgccg gagtcgcaac aacagcggtc
     1621 tggctgaata ccacggacga taacctgacc gatcctgaag gctccgacag tgcgggctgg
     1681 gtaaatcttc ttgaggatcc gaaaaggata ttcctgcggc agaagaacaa tctgtcagac
     1741 cttcagaata aagggacggc acgggataat cttcaggttt acagtaaaga gcagtcagat
     1801 caacgctatg ttcatcggga aggcgataaa ataaccggag agctgaaaat ccgtggtgtt
     1861 aatgcgctga ggattttcaa cgaagctttt ggcctgattt tt
//
LOCUS       DAJVQR010000245         1826 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVQR010000245 DAJVQR010000000
VERSION     DAJVQR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(208..609)
                     /locus_tag="OQM52_004200"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OQM52_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="OQM52_004201"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="OQM52_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCS1565799.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          570 a          330 c          340 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJVQR010000246         1808 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVQR010000246 DAJVQR010000000
VERSION     DAJVQR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            173..1198
                     /locus_tag="OQM52_004202"
     CDS             173..1198
                     /locus_tag="OQM52_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1565800.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1808
                     /locus_tag="OQM52_004203"
                     /pseudo
     CDS             1266..1808
                     /locus_tag="OQM52_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          504 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgtt
//
LOCUS       DAJVQR010000247         1753 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVQR010000247 DAJVQR010000000
VERSION     DAJVQR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..1753
                     /gene="sat"
                     /locus_tag="OQM52_004204"
                     /pseudo
     CDS             1..1753
                     /gene="sat"
                     /locus_tag="OQM52_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          664 a          295 c          337 g          457 t
ORIGIN      
        1 tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac agcggctctg
       61 gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca acccatggta
      121 ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat aaactggtta
      181 gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat gtaaccatta
      241 aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact caagaaaaaa
      301 taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt aaggataatt
      361 tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac ataaacggtc
      421 agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt gtaaactgga
      481 atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact ctgaatgttc
      541 aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt aatgaagaag
      601 gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta aataaagata
      661 attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt ggcacgctag
      721 atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat ggaacaacaa
      781 taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa gactcctaca
      841 tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat tctcaggata
      901 agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat gatgttgaag
      961 tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata ttcagaagca
     1021 cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc gttttgaaag
     1081 aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat aatcagcaga
     1141 cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca ttacacctga
     1201 acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata tcagcaaata
     1261 aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca gggaaaaata
     1321 ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc ataggagaaa
     1381 ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt gatcaagctg
     1441 tagttacact taatggtgca acctttctga ataatactcc tataagtata gataaaggag
     1501 caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc tccggtgaac
     1561 tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt ctccactacg
     1621 ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga aatatggcat
     1681 ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga cagcctgaaa
     1741 ctgaaacacc gac
//
LOCUS       DAJVQR010000248         1747 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVQR010000248 DAJVQR010000000
VERSION     DAJVQR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OQM52_004205"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OQM52_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCS1565801.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OQM52_004206"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OQM52_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1747)
                     /locus_tag="OQM52_004207"
                     /pseudo
     CDS             complement(1292..1747)
                     /locus_tag="OQM52_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          515 a          309 c          394 g          529 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatga
//
LOCUS       DAJVQR010000249         1702 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVQR010000249 DAJVQR010000000
VERSION     DAJVQR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..301)
                     /gene="pgrR"
                     /locus_tag="OQM52_004208"
     CDS             complement(<1..301)
                     /gene="pgrR"
                     /locus_tag="OQM52_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1565802.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITT"
     gene            439..1359
                     /locus_tag="OQM52_004209"
     CDS             439..1359
                     /locus_tag="OQM52_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1565803.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1359..1702
                     /locus_tag="OQM52_004210"
                     /pseudo
     CDS             1359..1702
                     /locus_tag="OQM52_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          465 a          397 c          418 g          422 t
ORIGIN      
        1 cagtagtaat acgtattgtc ccggatgggc ggttctgcag atcgctcagg gatgtcaggg
       61 cagaatctat gtcatgcaat atagggccaa gaacagacaa aagatgctcg cccgcttcag
      121 tgggtacaac gctgcgcgtg gttcgcgtca gaagccgcaa tcccaatcgt tcttctatac
      181 ggcgcactat ctggcttaaa gctgactgca ccatgctcag gcgggctgct gcgcgagtga
      241 agctacgctc ctctgcaacg acgacaaacg ccatcagatc agcgatttct tcacgcttca
      301 ttcagtatct ccatacaatc tattaatcat ttaaatgata ggtctaatca tgatttaggg
      361 tctaatcaac ttaatgatca atatctatag tgatatcacc aaccgtcaaa acgaaccgac
      421 aactccatga ggaactgaat gatgaataat aaagtcagct tcactaacag caataatcca
      481 accatctctt tgtctgcagt gatctatttc cctccaaaat ttgatgaaac ccgtcagtat
      541 ccggcaattg tggtatctca cccaggtggg ggcgttaaag agcaaacggc cggaacctat
      601 gccgaaaagc tggcggaaaa gggatttgtc actattgctt atgacgcatc ttatcaaggt
      661 gaaagtggcg gcgagccgcg tcagttagaa aacccataca ttcgtactga agacgtcagt
      721 gccgtgattg attacctgac aacgctttct tatgtcgaca atacccgaat tggtgcgatg
      781 ggaatttgtg ccggtgcagg atatacagcc aatgctgcaa ttcaggatcg ccgcatcaag
      841 gccatcggta ccgttagtgc ggtcaatatt ggttcgatgt tccgcaatgg ttgggaaaac
      901 aatgtgaaat ctattgatgc tctgccacac attgaagctg gctcaaacgc ccgaaccagc
      961 gatattagca gcgaagaata cgccattatg ccactggctc caatgaagga ggccgatgcg
     1021 ccgaacgaag aactgcgtca ggcctgggag tattaccaca cacctcgtgc gcagtatcca
     1081 acagcaccgg gttacgctac tttgcgcagc cttaaccaga ttattaccta tgatgcttac
     1141 catatggcgg aagtgtacct gactcagccg atgcagattg tggcgggcag ccaggcaggg
     1201 agcaaatgga tgagtgacga cctgtacgat cgggcatcaa gccaggataa acgctatcac
     1261 attgttgaag gcgcaaacca tatggatctt tatgacggca aagcctatgt ggctgaagca
     1321 atttcagtat tagctccgtt ctttgaggaa actctgtaat gaaacatatt caaatccgca
     1381 atagtgatat ggcctggcat attgctgcca atattcagtt tccaccaaat tttgatgagt
     1441 caaaacagta tccggcgatt atcagcgtcc atccttttgg cagttgtaag gagcagacgt
     1501 ctggcaatat ctatggcaag gcattggctg aaaaaggcta tgtcgttctc gcttatgacg
     1561 ccagtttcca gggagaatct ggtgggtagc ctcgttggat agaagatccg acacagcgcg
     1621 tagaggatat tagccgtgtt atcgattatg cagtaactct gccatatgtt gatgctgaac
     1681 gcattggcgt gctgggtgtg tg
//
LOCUS       DAJVQR010000250         1610 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVQR010000250 DAJVQR010000000
VERSION     DAJVQR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..543)
                     /locus_tag="OQM52_004211"
     CDS             complement(<1..543)
                     /locus_tag="OQM52_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCS1565804.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSVAVAEFFHKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DTKGEKRPMYQITKNGFVFLV"
BASE COUNT          444 a          374 c          368 g          424 t
ORIGIN      
        1 caccaggaaa acgaagccgt ttttggtgat ttggtacatt gggcgttttt cgcctttggt
       61 gtcggtgtag gtgacgtcct caaaattgag ggcgttaaat tcaggtgagc attcagtatt
      121 tgcgatggca cgtaacacat tgtcgtgtcg tttgtggaag aactctgcaa ccgcaacaga
      181 cgtagtgaca gcgcgaccgt tttcgatggt tacgtcaggg tgagaaaggg cagggatagt
      241 agccatgatg gcatcctcga gtgataagtt aattaactca ccaccgaggt tttccacgac
      301 cataagggtg gtgagacgta caggggtgga aataccggtc actcgagaac ccggccagtc
      361 ttgcgactgc cctgcacatc ccaccataat ttgaatggag ctgtgcatta cgcataaaaa
      421 aaccgcttca gcgcggctat gcgctcgagt aacgttcggg tttccacgcc cggcccccgt
      481 tttatgaggt gcagatgcac tataattcca cccgttctgg ttttcaatag ctacattcaa
      541 cattttctct tacctttcat caccgaagtg aactttgttg atgcggtgcc tggtgcctcc
      601 aggtgacgtt aaccagttaa caattaacgc cggatacagg gaaacccata accacccgat
      661 acgtgggaac taatcgcttt ttaactgttc cgcgtgcgct cagccgcatt caccgcatca
      721 caaaattcac tttaaaaagg gcggacatca gccagcaatg aaactgatgc cgccaaaggt
      781 accaatcaac atggagtgtt gtggcgtggt tgtcacttaa gcgtatggtc aacctgacaa
      841 cccggtgtcc tcaacgggga aggaataact ccgccatact taccgccgcg tcatttcgcg
      901 ttgtgtgcct gcttttaact acgtcaggcg aggtggttcc tgttattccc caacaacaag
      961 aaatttgtat aatctgaata ccccaacaat aagaagagtg agcaagtgat gtattagcga
     1021 tgtcagctct caaagagact gcatcaatgt taaaattatt caatgaagca aaaactcagg
     1081 ctgaggttga tcgcgctgtg tctgaaataa actctaagtt gtcaagcatt cagcttgaat
     1141 atttgtcatc tcattgatga gctgacaatc tctaaaagca atgaggctgc gctcaaagca
     1201 aaactcacgg agatagaaaa gtttaagttc caaagtgaag actgcgaacc tcgctaattg
     1261 attactggaa cgattgtgta tgccagacag tgcaatacta gctctggcaa gattaccatg
     1321 tatttttgtc cagaatgttt tgcgaagaga gcaatatcta tattgcaacc tttccctatc
     1381 gaggaaggcg ataaatatac gaaaactcaa tgcccctcct gtaaaaatga gtatctcatg
     1441 gactacaatt tcaataatga tttttgagtc acggtattca gatttttaaa gaacatgccg
     1501 gatactcacc cgtgtccggc gcacgcactc cacctcaccc gtggagaact ccttaattac
     1561 caaccttggc ttcgttggtt agccattaac gctggtatgt aatcattctg
//
LOCUS       DAJVQR010000251         1600 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVQR010000251 DAJVQR010000000
VERSION     DAJVQR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..360
                     /locus_tag="OQM52_004212"
     CDS             <1..360
                     /locus_tag="OQM52_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1565805.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OQM52_004213"
     CDS             361..726
                     /locus_tag="OQM52_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1565806.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OQM52_004214"
     CDS             723..1406
                     /locus_tag="OQM52_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1565807.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          452 a          324 c          438 g          386 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc
//
LOCUS       DAJVQR010000252         1551 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVQR010000252 DAJVQR010000000
VERSION     DAJVQR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            400..1149
                     /locus_tag="OQM52_004215"
     CDS             400..1149
                     /locus_tag="OQM52_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCS1565808.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1319..>1551)
                     /locus_tag="OQM52_004216"
     CDS             complement(1319..>1551)
                     /locus_tag="OQM52_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565809.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          425 a          329 c          315 g          482 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta t
//
LOCUS       DAJVQR010000253         1491 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVQR010000253 DAJVQR010000000
VERSION     DAJVQR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(1..265)
                     /locus_tag="OQM52_004217"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OQM52_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OQM52_004218"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OQM52_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCS1565810.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1177..1491)
                     /locus_tag="OQM52_004219"
                     /pseudo
     CDS             complement(1177..1491)
                     /locus_tag="OQM52_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          418 a          375 c          260 g          438 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gtgtctaaaa
     1141 agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca ggactaacag
     1201 ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac cgccagtttt
     1261 agtgttgtac gaataccaga gccagtgttg ttgcagcctt gctgccaacg aagcgccact
     1321 gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca gatgttatcc
     1381 gccggggcgg gagcttttta atgtccgtat gacggtattg atgccgattt tcagtgtcct
     1441 ggcggtatca cggccccctg cgccgttatg tgccatttca acgatctgct c
//
LOCUS       DAJVQR010000254         1487 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVQR010000254 DAJVQR010000000
VERSION     DAJVQR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(62..529)
                     /locus_tag="OQM52_004220"
     CDS             complement(62..529)
                     /locus_tag="OQM52_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1565811.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(595..834)
                     /locus_tag="OQM52_004221"
     CDS             complement(595..834)
                     /locus_tag="OQM52_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCS1565812.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(969..>1487)
                     /locus_tag="OQM52_004222"
     CDS             complement(969..>1487)
                     /locus_tag="OQM52_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1565813.1"
                     /translation="VINLSENGYRFLLLLLQGESDKQHIINEVWREQKGSVSDSSYYG
                     QIYMLRKALDVAGLSSSLIKTIPRKGVRYLGKVEKHVCENERDSDEDSSSYTNDSEAR
                     EVNTDANIPENDNSQEFSTEELLPIKHTDKNIEWYRTKQWSVFISVLTVLAVCWLTTL
                     VFLVLLFLNQDK"
BASE COUNT          480 a          328 c          261 g          418 t
ORIGIN      
        1 ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct gtcctaaaaa
       61 attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa attggtacag
      121 cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct gccagaacaa
      181 ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt tcatcaatag
      241 ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct gcggaaaaaa
      301 gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt tgatgctgta
      361 aaatgagtgc caaaaaaata agcacggtta acaccagtaa ataacgatgg ctaataattc
      421 tgagtaatat atccccccat gaaataaaaa taagaaagag cgcaacaaat aaaagatcga
      481 ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag gttgctgcca
      541 gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta taagttacgg
      601 attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt cgaatgcatc
      661 tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa gcagagttgc
      721 catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat ccttcgcgaa
      781 acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat tcatgttttc
      841 ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac cggtatccca
      901 tatttttggg atgtggctat aattatctat tcccgctatt tagtcttcaa aaaaaattac
      961 atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag cgtcgtcagc
     1021 cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt cgttctatac
     1081 cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga gaattcctgg
     1141 ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc gctatcgttg
     1201 gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg tttctcgacc
     1261 tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact cgacaaacct
     1321 gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct gtcgcttacc
     1381 gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga ttctccctgt
     1441 agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattac
//
LOCUS       DAJVQR010000255         1480 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVQR010000255 DAJVQR010000000
VERSION     DAJVQR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            206..457
                     /locus_tag="OQM52_004223"
     CDS             206..457
                     /locus_tag="OQM52_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565814.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            524..802
                     /locus_tag="OQM52_004224"
                     /pseudo
     CDS             524..802
                     /locus_tag="OQM52_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            804..>1480
                     /locus_tag="OQM52_004225"
     CDS             804..>1480
                     /locus_tag="OQM52_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1565815.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          362 a          375 c          388 g          355 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg gttttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctctatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatcgg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aacgtgcggg tattacttcg acctgttctg gttccggaac
      841 tcgggctggt ggtccttaag cccggtcgtg aatccatgcc ggtattccac aatacccggg
      901 tactggtgga gccggaaccg aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg
      961 ttcgccagcc gctagtggaa gacaaaacat tgctgccgtt tttcagtaac gcacgggtga
     1021 ttcgtgctgc tggtggtgct ggcgcattgt ctgactggct gttgcgccat attaaatcct
     1081 gccagtggcc acacggcgat tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg
     1141 gcgcaatggt attgtgctgg cactgcgaca accagctgcg tgaccagaca tccgaatcac
     1201 tcgagcaact tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa
     1261 taagcggtac gcaggagcgt gaattatctc tggctgaatt atcctggtgg gcggtccgca
     1321 atcaggtggc ggacgcgcta ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg
     1381 aaaaaatccg ctcaatgtac cgtgaaagcg acatcgtgcc gggagagcag accgccacca
     1441 gcatactgaa gcagcgcaca aaaaatcttg cgccgctgcc
//
LOCUS       DAJVQR010000256         1470 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVQR010000256 DAJVQR010000000
VERSION     DAJVQR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(5..184)
                     /locus_tag="OQM52_004226"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="OQM52_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="OQM52_004227"
     CDS             708..992
                     /locus_tag="OQM52_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCS1565816.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="OQM52_004228"
     CDS             992..1267
                     /locus_tag="OQM52_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1565817.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          401 a          368 c          361 g          340 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga
//
LOCUS       DAJVQR010000257         1462 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVQR010000257 DAJVQR010000000
VERSION     DAJVQR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            240..509
                     /locus_tag="OQM52_004229"
                     /pseudo
     CDS             240..509
                     /locus_tag="OQM52_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OQM52_004230"
                     /pseudo
     CDS             506..732
                     /locus_tag="OQM52_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OQM52_004231"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OQM52_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCS1565818.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          425 a          290 c          348 g          399 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc ag
//
LOCUS       DAJVQR010000258         1443 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVQR010000258 DAJVQR010000000
VERSION     DAJVQR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            82..1275
                     /locus_tag="OQM52_004232"
     CDS             82..1275
                     /locus_tag="OQM52_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCS1565819.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="OQM52_004233"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="OQM52_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          422 a          313 c          339 g          369 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DAJVQR010000259         1432 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVQR010000259 DAJVQR010000000
VERSION     DAJVQR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(119..310)
                     /gene="csgC"
                     /locus_tag="OQM52_004234"
                     /pseudo
     CDS             complement(119..310)
                     /gene="csgC"
                     /locus_tag="OQM52_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
     gene            382..>1432
                     /locus_tag="OQM52_004235"
     CDS             join(382..631,631..>1432)
                     /locus_tag="OQM52_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCS1565820.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAIT"
BASE COUNT          388 a          343 c          375 g          326 t
ORIGIN      
        1 attctatttc ctcaatgatt agtcatcctt gaaggttgtg ttatccatac tttcaggttg
       61 gctgattcgc agcagaccat tctctccaga ttcatcttat gctcgatatt tcaacaaatt
      121 aagacttttc tgaagagggc gtccattgtt gtgataaatg aagtgcctgt ccatcagaaa
      181 cagtaacaac tattttcacc cgatcttccg gggaaatatt taaactcaac ttcgtcaaag
      241 caatgggttg attagcaggc aatgagaggg tcttttcttg cttcgtctga ctttgccctg
      301 aactgccttt gaagtggcac actgaatttg gccacctgaa cagaggtgat atgctcacct
      361 cagaacaaca caggtgctcc aatgaaaaaa agaaatttta gcgcagagtt taaacgcgaa
      421 tccgctcaac tggttgttga ccagaaatac acggtggcag atgccgccaa agctatggat
      481 gttggccttt ccacaatgac aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa
      541 acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct caggaaaaag
      601 ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct cttgatgtca
      661 gactccctga acagttctcg ataatcggga aactcagagc gcattatcct gtggtcacac
      721 tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac cgtcctgaaa
      781 aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat ggcatcagcc
      841 acggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc taccagatgg
      901 gacgctggct tgctggcagg ctcatgaaag agctggggct ggtcagctgt cagcagccga
      961 ctcaccggta taaacgtggt ggtcatgaac atgttgctat ccctaactac cttgaaaggc
     1021 agttcgccgt gaccgagcca aatcaggtgt ggtgcggtga tgtgacctat atctggacgg
     1081 gtaagcgctg ggcgtacctc gccgttgttc tcgacctgtt cgcaagaaaa ccagtgggct
     1141 gggccatgtc gttctcgccg gacagcaggc tcaccatgaa agcgctggaa atggcatggg
     1201 aaacccgtgg taagcccggc ggggtgatgt tccacagcga tcagggcagt cattatacga
     1261 gcaggcagtt ccggcagtta ttgtggcgat accagatcag acagagtatg agccggcgcg
     1321 gaaactgctg ggataacagc ccaatggaac gcttcttcag gagtctgaag aacgaatgga
     1381 tgccggtggt gggttacgta agcttcagcg aggcagctca cgccataacg ga
//
LOCUS       DAJVQR010000260         1394 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVQR010000260 DAJVQR010000000
VERSION     DAJVQR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..157
                     /locus_tag="OQM52_004236"
     CDS             <1..157
                     /locus_tag="OQM52_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCS1565821.1"
                     /translation="STPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKER
                     RKEWGTL"
     gene            142..780
                     /locus_tag="OQM52_004237"
     CDS             142..780
                     /locus_tag="OQM52_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCS1565822.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1008..>1394
                     /locus_tag="OQM52_004238"
     CDS             1008..>1394
                     /locus_tag="OQM52_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565823.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          406 a          253 c          345 g          390 t
ORIGIN      
        1 cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga atgatttctg
       61 gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt acctgcagcg
      121 tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact tggcaacatg
      181 attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc tctgaatcat
      241 ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga ctgtgtgttg
      301 tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat ggcactaggt
      361 gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc ggtggtggtg
      421 tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt actggggcgg
      481 gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat taatccggag
      541 agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc cagggggaaa
      601 caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg ctggacgaat
      661 gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct gaaacagatt
      721 agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac ggttcgttga
      781 tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa aaatgatacc
      841 gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta ggctttgata
      901 acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg cagatttaca
      961 aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg aaaaaacaga
     1021 tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg aaaatattta
     1081 aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc gaaccattcc
     1141 acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata tttattaata
     1201 aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa agaagagata
     1261 tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta tttgatcatg
     1321 acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat tatgttttga
     1381 ttgatttttc tagt
//
LOCUS       DAJVQR010000261         1321 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVQR010000261 DAJVQR010000000
VERSION     DAJVQR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>1321
                     /locus_tag="OQM52_004239"
     CDS             <1..>1321
                     /locus_tag="OQM52_004239"
                     /inference="COORDINATES: protein motif:HMM:NF017826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCS1565824.1"
                     /translation="KKIHNFFAIPYLKLSTAKQSSGVKTAETNTGYTGVRFLNNDLEI
                     NEIALSSSERAEFTSSKSRLTANDWTPPAGSDRSDVYKAFMEAAQAGYEPHVRPNGKI
                     YPAFIKRMTGYTQRGWVTPDLHIRSKHDLYVGKVATQSEKMGDPEIQKQVLREIIKQS
                     PAVRIFLAGTFAAYTKGRICISDEFCPIVNYVGEGEKGKSTTLRICASIEGSPSKGKM
                     VIEGVSTEAGVENFATAYNHGPVFLDEIDELLRMNELSKRIMRLTNGGGRVIYDTEKT
                     FKQPNVWNGMFFTTSNTPLKDGVRGDGKETAILTRLIEININDPDLHIFYPDKNDGGK
                     VNEWMSKLAVNYGNIYDEIIQYIINNADELEEKLEDFESQLLTTEELRYFKKYRRSAH
                     TLALVMLGAEMMEPILGKESSMLTHDAITIYKNKYAQDVNDEIDYADY"
BASE COUNT          416 a          252 c          284 g          369 t
ORIGIN      
        1 gaagaaaata cataacttct ttgctatacc atacctcaag ctttctacag ctaaacagag
       61 ttctggcgtt aaaacagcag agactaacac tggatatact ggcgttcgct tcctgaataa
      121 cgatttagaa ataaatgaga tcgcgctctc ttcatccgaa cgagcagagt ttaccagctc
      181 taaatctcgc ttaaccgcta atgactggac acctccggcg ggttccgacc gttcagacgt
      241 ttataaagcc tttatggagg cggcacaagc cgggtatgag ccacatgtaa gacctaacgg
      301 taaaatctat cctgcgttca taaagcgtat gacgggctac actcaaagag gctgggtgac
      361 tcctgacctt catatccgtt ctaagcatga tctgtatgtt gggaaggtag ctacccaaag
      421 tgaaaagatg ggtgatcctg aaatccagaa gcaggttctt agagagataa tcaaacagtc
      481 tccagcggtt cgtatcttct tggctggcac gttcgcggca tacactaaag gccgtatctg
      541 tatctcagat gagttttgcc cgatagttaa ctatgttggt gaaggtgaga aaggaaaatc
      601 taccacgctt cgtatatgcg cctctattga gggttctcct tctaagggta aaatggttat
      661 agaaggtgtc agtacggaag caggtgtaga gaacttcgca actgcttaca accacggccc
      721 tgttttcctt gatgagattg acgaacttct caggatgaat gagttatcaa aaaggataat
      781 gcgccttacc aacggcggcg gtagagttat ttatgatact gaaaaaactt tcaaacagcc
      841 taacgtctgg aacggtatgt tctttactac ctcaaataca ccgctaaaag atggtgttag
      901 aggggatgga aaagaaacag ctattcttac tcgtcttatt gaaatcaata tcaatgatcc
      961 agaccttcac atcttttatc ctgataaaaa tgatggtggt aaagttaatg agtggatgtc
     1021 aaaacttgct gttaactacg gtaatattta cgatgaaata attcagtata ttatcaataa
     1081 cgctgacgag cttgaagaaa agcttgaaga ttttgagtct caattattaa caacagaaga
     1141 gttaaggtat tttaaaaaat atcgtcgttc agcgcataca ttagcattag taatgcttgg
     1201 tgctgaaatg atggagccaa ttttaggcaa agaaagttct atgttaactc atgacgccat
     1261 tactatttat aaaaataaat atgcgcaaga tgttaatgat gaaattgatt atgctgatta
     1321 c
//
LOCUS       DAJVQR010000262         1307 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVQR010000262 DAJVQR010000000
VERSION     DAJVQR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..626)
                     /locus_tag="OQM52_004240"
     CDS             complement(<1..626)
                     /locus_tag="OQM52_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1565825.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1300)
                     /locus_tag="OQM52_004241"
     CDS             complement(740..1300)
                     /locus_tag="OQM52_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1565826.1"
                     /translation="MIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSDTADQ
                     IVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGT
                     PLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIA
                     RYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          355 a          303 c          280 g          369 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgc
//
LOCUS       DAJVQR010000263         1301 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVQR010000263 DAJVQR010000000
VERSION     DAJVQR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OQM52_004242"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OQM52_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCS1565827.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          364 a          234 c          292 g          411 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 ccccttacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtcgtg agccgttttc agacaatcgc a
//
LOCUS       DAJVQR010000264         1275 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVQR010000264 DAJVQR010000000
VERSION     DAJVQR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            34..435
                     /locus_tag="OQM52_004243"
     CDS             34..435
                     /locus_tag="OQM52_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1565828.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OQM52_004244"
     CDS             447..818
                     /locus_tag="OQM52_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565829.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OQM52_004245"
     CDS             805..>1275
                     /locus_tag="OQM52_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565830.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJVQR010000265         1200 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVQR010000265 DAJVQR010000000
VERSION     DAJVQR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(15..404)
                     /locus_tag="OQM52_004246"
     CDS             complement(15..404)
                     /locus_tag="OQM52_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565831.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            622..1062
                     /locus_tag="OQM52_004247"
     CDS             622..1062
                     /locus_tag="OQM52_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1565832.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          348 a          298 c          270 g          284 t
ORIGIN      
        1 atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct
       61 agacattgcc agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac
      121 atgttattgc gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca
      181 gaattaatat tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag
      241 gatgcagtat aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa
      301 ctacctcccg aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg
      361 tgaccaaaac caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg
      421 acttttaaaa acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat
      481 actacgcccg tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg
      541 gcagcctcag accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac
      601 acaggagaca ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca
      661 ggaaagtcgc gctcgcaatt atatcgtgac atggctaaag gccttgtcag ctatcgcaca
      721 ggaaaaaatg atcgccggga gttcgagacc agtgaactga ttcgggccta tggcgagctg
      781 cgcacttctg agacaccaaa aaggcacaag gagggacact ccaaatctgt cgttagccag
      841 gcggaaaatg ctcagctaga cgtcattcag cagaaactgg acgctctgca gcagactgtt
      901 accctaatgc ttgaagacaa gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag
      961 agggaacaac tacaggccga aattagccgt ctggctgacg cgctggcaca ggaaaaaaag
     1021 cggggattct ggtcgcggct gtttcgctcc ggttcagagt aacagtgcag gtgactctga
     1081 tttaccataa catctgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga
     1141 aaccggtaaa acaggcaaca aaccacccga agacccgcca gaaacgcgca ggctctgttt
//
LOCUS       DAJVQR010000266         1199 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVQR010000266 DAJVQR010000000
VERSION     DAJVQR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..496
                     /locus_tag="OQM52_004248"
     CDS             <1..496
                     /locus_tag="OQM52_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1565833.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            560..>1199
                     /locus_tag="OQM52_004249"
     CDS             560..>1199
                     /locus_tag="OQM52_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1565834.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSE"
BASE COUNT          283 a          285 c          388 g          243 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg tggcggcagc
      961 cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa gcccgacagg atgcaaccgt
     1021 cacagctgag gacagaaagg ccactgcgga agatgtgaca agcacaggag caaatgcagc
     1081 cgcagccgga cagagcgcac aggatgccgc aggttatgcc cgcgcagcag aacaggccaa
     1141 aaatgacatt gatgctgcgc tgaccggcac tctgaaaatg gctaaccatc tgtcagaaa
//
LOCUS       DAJVQR010000267         1140 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVQR010000267 DAJVQR010000000
VERSION     DAJVQR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>1140
                     /locus_tag="OQM52_004250"
     CDS             join(<1..233,233..>1140)
                     /locus_tag="OQM52_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1565835.1"
                     /translation="GKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIKKY
                     GPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNSCC
                     WPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLD
                     LRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPDER
                     WVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPEKQ
                     VLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIY
                     GTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT          303 a          272 c          306 g          259 t
ORIGIN      
        1 cggtaagcgt tatcccgaag agtttaaaac tgaagcagtc aaacaggttg ttgatcgcgg
       61 ttattctgtt gccagcgttg caacacgtct cgatatcacc acccacagcc tttatgcctg
      121 gataaagaag tacggtccgg attcttccac taataaagaa cagtcagatg ctcaggccga
      181 gatccgccgt ctccagaaag agctgaaacg ggttaccgac gaacgggaca tattaaaaaa
      241 agccgcggcg tacttcgcaa agctgtccga ctgaggtacg cctttatccg tgacaactcc
      301 tgttgctggc ctgttcgcct gctctgtcgg gtgctggatg ttcatcccag tggtttttac
      361 gcctggcttc agcagccgca ttcacaacgc catcaggcag acctgagact gacaggacag
      421 attaaacagt tctggctgga atcgggatgc gtctatggtt atcgcaaaat ccatctggat
      481 ctgcgtgaca gcgggcaaca gtgcggagta aacagagtct ggagactgat gaaacgtgtc
      541 ggaataaagg ctcaggtcgg ataccgaagc ccgcgggcac gtaaaggcga ggccagtatc
      601 gtgtcaccca acaggctcca gcgacagttc aatccggatg ctcctgatga gcgttgggta
      661 acggacataa cctacatcag gacccacgaa ggctggctgt atcttgccgt tgttgttgat
      721 ctgttctcac gcaaaattat cggctggtcc atgcaatccc ggatgacaaa ggacattgtc
      781 ctgaacgcac tgctgatggc tgtatggcgg cgtaatcccg aaaaacaggt gctggttcat
      841 tcggatcagg gcagtcagta cacaagccat gagtggcagt cgttcctgaa atcacacggc
      901 ctggagggta gcatgagccg tcgcggtaac tgccatgata atgcggttgc agaaagtttt
      961 ttccagttgt tgaaacgtga acggataaag aaaaagatct acggaacgcg ggaagaagcc
     1021 cgcagtgata tttttgatta catcgaaatg ttttataaca gtaagcgtcg gcatggttct
     1081 agcgaacaga tgtcaccgac agaatatgaa aaccagtatt atcaacggct cggaagtgtc
//
LOCUS       DAJVQR010000268         1116 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVQR010000268 DAJVQR010000000
VERSION     DAJVQR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>1116)
                     /gene="yiaN"
                     /locus_tag="OQM52_004251"
     CDS             complement(<1..>1116)
                     /gene="yiaN"
                     /locus_tag="OQM52_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCS1565836.1"
                     /translation="DSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGY
                     VGVLAAMIMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPS
                     IPFIIFGVSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWH
                     SFVSGIWALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLI
                     GAAKTTSVVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVG
                     MVMDLTPTVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVA
                     KLKFDDAVRGVFPYVLVLYSLLVVFVFIPDLIILPLKW"
BASE COUNT          343 a          282 c          285 g          206 t
ORIGIN      
        1 ccatttcaaa gggaggatga tgagatcggg aataaaaaca aatacgacta ataatgaata
       61 taaaaccagg acgtaaggga aaacgcctct gactgcatca tcgaatttga gttttgccac
      121 ccctgaaata acgttaagta cattaccgat aggcggtgta attaagccga ttgagcagtt
      181 aatgataaac atgacaccga aataaatcgg atcgattcct gcctctttaa ctaaaggcat
      241 taataccggg gtaagaatta ataccgtcgg cgttaaatcc atgaccatgc cgacaatcag
      301 gatagccacc ataatgacga taaacagcag acgcggtgaa tcgaccaacg gttgcagtaa
      361 atcagaaacc atcatcggca gttcagcaat ggtaatcagc caggcggaaa cttgtgccga
      421 ggccaccaga aacataacca ctgaggtggt tttcgccgcg ccaatgagta catgccaaag
      481 tgtggcgaag gtcatttcac ggtaaataac tgtggcgaca aacagcgcat agaaagcagc
      541 aacggccccg gcttcggtag gggtaaacag accggagcga aacccgccaa taatgatgac
      601 tggaagaaac agcgcccaga taccggagac aaaagagtgc cagatttcct gcatcgttgc
      661 tttttgctgg cggggtaaat taaggcgact ggcctgccgc caccaggtaa gcatcagcgt
      721 tgcgcccatc attatgccag gggcaatgcc cgccataaac agcttactga tggataatcc
      781 gctggaaaca ccgaagataa taaacggaat ggaaggggga ataattggcg cgataatgcc
      841 gccagaggca atcagccccg ccgcccggtt gaccgggtag ttggcgctgc gcatcatcgg
      901 caccagtaga gcggcaacgg cggcggtatc cgcaacggca gaaccagaaa ggctcgccat
      961 aatcatagct gccagcacgc cgacgtagcc cagcccgcct ggtttatgcc ccaccagttt
     1021 catcggcagg tcaacaatgc gttttaacaa gccgcccgca ttcatgattt cacccgccag
     1081 cacaaagaac ggaatcgcca ggagggagaa gctatc
//
LOCUS       DAJVQR010000269         1100 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVQR010000269 DAJVQR010000000
VERSION     DAJVQR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            55..>1100
                     /locus_tag="OQM52_004252"
     CDS             55..>1100
                     /locus_tag="OQM52_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1565837.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJVQR010000270         1081 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVQR010000270 DAJVQR010000000
VERSION     DAJVQR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OQM52_004253"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OQM52_004253"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS1565838.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="OQM52_004254"
     tRNA            complement(986..1062)
                     /locus_tag="OQM52_004254"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          283 a          253 c          246 g          299 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 c
//
LOCUS       DAJVQR010000271         1080 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVQR010000271 DAJVQR010000000
VERSION     DAJVQR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            92..610
                     /locus_tag="OQM52_004255"
                     /pseudo
     CDS             92..610
                     /locus_tag="OQM52_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          329 a          242 c          227 g          282 t
ORIGIN      
        1 aatctgccgg ttacgccgaa tctgctgaat cagaacttta cgcccacagc acctaatcag
       61 gtctgggtcg cagattcagt tgtccaggcc ttccgaaatc aaccgactga aggcgctggt
      121 cgcgaaactg cagcgtatgc agttcggtaa aagctcagaa aaacttcgcg caaaaaccga
      181 acggtggata caggaagcac aggagcgaat cagcgcactt caggaggaaa tggcggaaac
      241 actgggtgag caatatgacc cggtactgcc atcttccctg cgccagtctt cagcccgtaa
      301 accgttacca gcctcacttc cccgtgcccc ccgggtcatc cggccggaag aggaatgctg
      361 tccggcctgt ggtggtgaac tcagtccact gggatgtgat gtgtcagagc agctggagct
      421 tatcagcagc gcctttaagg ttatcgaaaa acaacgccct aaactggcct gtcgccggtg
      481 cgaccatatc gtgcaggcac cggtgccttc aaaacccatt gcacgcagtt atgccggagc
      541 ggggcttctg gcccatgttg ttaccggaaa gtatgcagac catctgccat tataccgcca
      601 aagtgatcta ctattccaca cagctattta gctctctttt tgttgagctc ccatgattct
      661 ttcatttatt gttactaatt attttatgtt atcatgatgt tagaacacgc tctgctctgt
      721 gtctctattt tatctatcat attctgagga ttaactacat aacaaggcgc aagatatata
      781 tacaaaaaca aataataccg ttgcaataat tccttttagt aaaggaggtg tgattaattt
      841 caggggattt cagagtaaaa attgtacaac atattaaaat atgttcattt cgttataata
      901 cacacaagga aatattaagt tagataatat attctaaata accatagata aaaacgcaca
      961 taatggggaa tattttatga aaatacctga agcagtaaat catattaatg ttcagaataa
     1021 tattgatctt gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaat
//
LOCUS       DAJVQR010000272         1077 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVQR010000272 DAJVQR010000000
VERSION     DAJVQR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..732
                     /locus_tag="OQM52_004256"
     CDS             <1..732
                     /locus_tag="OQM52_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCS1565839.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASG
                     VHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDR
                     PLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(734..960)
                     /locus_tag="OQM52_004257"
                     /pseudo
     CDS             complement(734..960)
                     /locus_tag="OQM52_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          286 a          260 c          234 g          297 t
ORIGIN      
        1 aaaaccgaca gaatccattt gaaagacgca gcgcgaatgc tggaagtttc tgtaatgact
       61 attcgtcgcg atctccatca ggaagatgaa cctctgccac tgaccctact gggtggctat
      121 attgtaatgg tgaataaacc cgcgccatcc atgccagtaa tccatgacgt tccaaaaaat
      181 catcgtgatg acttacctat tgcaattctg gctgccggaa tggttaatga aaatgatctg
      241 atcttctttg ataatggcca ggagatacca ctcgttataa gcatgatccc ggatgcaatc
      301 accttcaccg gcatctgtta ctcacatcgc gtctttgttg cgttgaatga aaagcctaat
      361 gtaacagcaa tactttgtgg tggtacgtat cgtgccagaa gtgatgcttt ttacgatgcc
      421 agtaactctt cgccattaga ctctctcaat ccgcgaaaaa tatttatttc cgccagcggt
      481 gtgcataatc actttggcgt cagctggttt aaccctgaag atcttgccac taagcgtaaa
      541 gtgatgaacc gtggactacg gaaaattttg ctcgcccgcc acgcgttgtt cgatgaagtg
      601 gcctctgcca gcctcgcacc gatctctgca tttgacgttc tgattagcga tcgtccgtta
      661 ccggcagatt atgttacgca ctgccagaat ggttctgtaa agatcattac acctgattca
      721 gaagacgaat gacttactga aaaaacacca caatcttgtt aaacatcgtc gggttggact
      781 gattacgttg cactttcacc acatattcca gcttatctat ttggcttatc acctactcca
      841 gacgctggtc atccttgacc agtagccaga tatggctttt gtcgcagtcc tgaatcggca
      901 ggcagagaat gtcttcaacg ttaagagcgc gacgggcaaa agcccacaaa ggtgagtcat
      961 tacacccgga tggtaaacag gcacttcctt gttgtagaag ccgttatatt gtccataatg
     1021 tctgggtttt taatgcatac tgggtaacaa ccggacgaat cttcgaatat cactggc
//
LOCUS       DAJVQR010000273         1072 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVQR010000273 DAJVQR010000000
VERSION     DAJVQR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(1..254)
                     /locus_tag="OQM52_004258"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="OQM52_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(302..487)
                     /locus_tag="OQM52_004259"
     CDS             complement(302..487)
                     /locus_tag="OQM52_004259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565840.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            650..931
                     /locus_tag="OQM52_004260"
                     /pseudo
     CDS             650..931
                     /locus_tag="OQM52_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT          247 a          321 c          235 g          269 t
ORIGIN      
        1 ttctcactgt ctccgttatg gcgtacacca catcttccag taatcgccgc ttcaccggac
       61 tcaggacccc gtatcaccat ccggaacccc ttatccgcca catgccagga gaacctttcc
      121 ataaactcgt cgccactcat cacaaggttt tcttctcgtt ttgtccggtg gctgttgtaa
      181 cgcaggccaa tttcatcctg accggcataa tgttccaggc ggctcatctg caccggctgc
      241 ttctccagcc agttaagcca tcggtttccc tgctgaaaaa tatcggcaaa acggggaagc
      301 atcagaaagg cggaataacg ccatccggcc agtgaaccgt accgcattca gggcaataca
      361 tgccgtcggc gttgataccg gcaggaattg tcgcaaattc ccgctccgtg cagcgggcaa
      421 tttccggata cccttcgtca tcaacacgta caaaccagaa gaccagtttt ttgtttccgg
      481 catccacaaa gaacggaata ttcaggtctg cgcaacactc aacggcatcg tcaaaattat
      541 caaagcgcag aacctcagct tcttcgtaac cggctcattt aaaccgtctg gtctgtttcc
      601 tccggctcca caaaaataat gtccatcatt ttgaagtgga cactatcgta tggaataccg
      661 gacctggatc actgaagctt tacgccttca tttcgaagaa catttaccac gggttgtagc
      721 cggacgtcgc ctgggcgtac caaaatcaac tgtctgtggt atgttcgtgc gcttccgcaa
      781 cgctggcctt tcatggcctc tgcccgcagg tatgtcggag caggaacttg atgcccttct
      841 ttacgaaagt gcctccacag tacctgtcgt acttactgaa agcaccgtta tgcccaaact
      901 cccggtggta aaaaaacgtc cccggcggcc ttaatgccag tcagttaagc aactgactgg
      961 ctctttttcg gggctgtggg gtatttccag ggcctctcct ttaccactct cgggaaggcc
     1021 cttccccttc tcgtcggtaa tttcacaagt tgtcccatac ttgcaagatc gc
//
LOCUS       DAJVQR010000274         1067 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVQR010000274 DAJVQR010000000
VERSION     DAJVQR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            70..249
                     /locus_tag="OQM52_004261"
     CDS             70..249
                     /locus_tag="OQM52_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565841.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            301..495
                     /locus_tag="OQM52_004262"
     CDS             301..495
                     /locus_tag="OQM52_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565842.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          322 a          245 c          247 g          253 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat tgctttacga
       61 gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact gcaacaccgt
      121 atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga cccattgcga
      181 tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa aacacgtcca
      241 ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga ggaaaaccgg
      301 atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca acgggagttt
      361 gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt tccacttgaa
      421 gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt cttagaatgc
      481 aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg gcatcgttat
      541 cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct ttgtttgccg
      601 gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg acattgtaat
      661 aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat cgttgacaat
      721 atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct gaagcatccg
      781 acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg gaactttgac
      841 aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg gaaaagtggt
      901 acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag cttgtcagaa
      961 aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag tgcccggcca
     1021 gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaat
//
LOCUS       DAJVQR010000275         1021 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVQR010000275 DAJVQR010000000
VERSION     DAJVQR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..179
                     /locus_tag="OQM52_004263"
     CDS             <1..179
                     /locus_tag="OQM52_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1565843.1"
                     /translation="DGSRGRRAKSIAFANMDETEFQQVYKSVLNVLWNWILFRKFSSP
                     EEVENVAAQLLEFA"
     gene            179..469
                     /locus_tag="OQM52_004264"
     CDS             179..469
                     /locus_tag="OQM52_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS1565844.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            466..1020
                     /locus_tag="OQM52_004265"
     CDS             466..1020
                     /locus_tag="OQM52_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1565845.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMCDAFSSGDLKTVC"
BASE COUNT          280 a          207 c          296 g          238 t
ORIGIN      
        1 ctgatggtag tcgtgggcgc agggcaaaat ctatcgcatt tgcgaacatg gacgaaaccg
       61 agtttcagca ggtttataaa tctgtactga atgtgctgtg gaactggatc ctgttccgta
      121 aattttcctc tccggaggaa gtcgaaaatg tggccgcgca gttactggag tttgcgtaat
      181 ggtggattta cgtaaagcgg cgcgggggca gatgtgcacc gtcagaattc ctggctactg
      241 caatcacaat cccgaaactt ctgtgctggc gcattacagg ctggcgggga cgtgcggaac
      301 agcgacaaaa ccacacgata tgcaggcagc gattgcctgt agctcatgcc acgatttaat
      361 cgacgggcgg gtaaaaacca gcgattacac caaagaagaa ttacgcctga tgcatgcaga
      421 aggtgttttt cgcacacaag aaatctggag aaaggaaggt tatttatgat ttacccaaca
      481 aatacaggca aaagcgggga acaccttcgt ctcaccacgc tggaaagtgt ctggattcag
      541 ggaaaactgc gcatgtgggg gcgctggtcg tatattggcg gcggtaagac gggaaatatg
      601 tttaaccagt tgctggcatc caaaaaattg acgaaaacag ccgtcaatga agccctgcgc
      661 aggatgaaaa aagcgggaat agagaaacct gagctggaag cgtttttgcg agagatgatc
      721 aatggcaagc aaaagacctg gctggcgcat tgtactgatg cagaggcgtt atgtattgat
      781 agagtcataa gtgaggtgct ggcagagcat cctggattga ttagtgtcct ccggcaacgg
      841 tatgaagggc gggggatgac taagcgtaaa atggctgaat tgctgaatga cgcacaccct
      901 gagtggtgtt tccggacgtg ctgcagtcgg gtagatgtat ggctaaatct tgctgaatat
      961 atgctctatc taccgatgtg tgatgcattc tcttccgggg atctaaaaac cgtttgttga
     1021 c
//
LOCUS       DAJVQR010000276         1001 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVQR010000276 DAJVQR010000000
VERSION     DAJVQR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(142..478)
                     /locus_tag="OQM52_004266"
                     /pseudo
     CDS             complement(142..478)
                     /locus_tag="OQM52_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          332 a          180 c          178 g          311 t
ORIGIN      
        1 agaacttctt cagcacactg ctaaaacgca tagtgatcga gcgctgattc tggcgaacaa
       61 ctgaactaat acatcagaat ctgcattatg ttaaataaat atgaaaagat ggtttaaata
      121 ccccgttact tttgacttac actatacggt atcgcatcgt ttaatattcg caccggccag
      181 atttttattt ctattagttg tcacaatact gaatgcgtac gaccacagta ttctggctcc
      241 tgtgtggtta tgctttaatt ctgcgttccg ggcagataag cagttgcttg caggaatcct
      301 tcttgtgtta atgtcagttc ccctttttac cagtgctgat ttccacattc cgtccaacag
      361 agcttatagc ctttccctgg attatagcat tgtccggctg aagttctttt tgaataataa
      421 tagaagcact gctggcagat ccagtccgtt tttcataacc cactgtactg ataaccataa
      481 tctaatcagt agaaattgag tcgaaaataa gcactactct atacaggata attagaggtc
      541 agtttgatta ttcacaattc atcatcagca ttttctattt ctgacgaaat caacatgaaa
      601 ataaccatat atgataatta ttataataac ggctttaatt ggaatacata tattacaacg
      661 tattatatat aattggtatt ctgggaacta tattctcaaa atacagtaga aacgaggtat
      721 gtttctggtg gaaaggacag tgggattaaa aagtaagaat tgataaaaaa aacgccagca
      781 acacatagtg ccggcggagg gaatacccca tggagaaaat gtgatgccta gagcatcatg
      841 aatatataat taaaaatagt tagcgttgtc aatactttca caaaaataat tttcgtagta
      901 tagaaagata tttttatgca tgacctacct gaatttgctc cgggtagagg ttataaataa
      961 aaattgaatc acgacaaaca caatattcat agtatggcga t
//
LOCUS       DAJVQR010000277          956 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVQR010000277 DAJVQR010000000
VERSION     DAJVQR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>956
                     /gene="cadB"
                     /locus_tag="OQM52_004267"
     CDS             <1..>956
                     /gene="cadB"
                     /locus_tag="OQM52_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCS1565846.1"
                     /translation="NLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYA
                     GEISPAFGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVF
                     TFVNMLGGTWVSRLTTIGLVLVLIPVVMTAIVGWHWFDAATYAANWNTADTTDGHAII
                     KSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQVLSGMYPS
                     SVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNF
                     PKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSAGGKAS"
BASE COUNT          193 a          220 c          247 g          296 t
ORIGIN      
        1 aacctagcaa gtatcggtgg tattgctatc tggggttgga ttatctctat tattggtgca
       61 atgtcgctgg cgtatgtata tgcccgactg gcaacaaaaa acccgcaaca aggtggccca
      121 attgcttatg ccggagaaat ttcccctgca tttggttttc agacaggtgt tctttattac
      181 catgctaact ggattggtaa cctggcgatt ggtattaccg ctgtatctta tctttccacc
      241 ttcttcccag tattaaatga tcctgttccg gcgggtatcg cctgtattgc tatcgtctgg
      301 gtatttacct ttgtaaatat gctcggcggt acctgggtaa gccgtttaac cactattggt
      361 ctggtgctgg ttcttattcc tgtggtgatg actgctattg ttggctggca ttggtttgat
      421 gcggcaactt atgcagctaa ctggaatact gcggatacca ctgatggtca tgcgatcatt
      481 aaaagtattc tgctctgcct gtgggccttc gtgggtgttg aatccgcagc agtaagtact
      541 ggtatggtta aaaacccgaa acgtaccgtt ccgctggcaa ccatgctggg tactggttta
      601 gcaggtattg tttatatcgc tgcgactcag gtgctttccg gtatgtatcc gtcttctgta
      661 atggcggctt ccggtgctcc gtttgcaatc agtgcttcaa ctatcctcgg taactgggct
      721 gcgccgctgg tttctgcatt caccgccttt gcgtgcctga cttctctggg ctcctggatg
      781 atgttggtag gccaggcagg tgtacgtgcc gctaacgacg gtaacttccc gaaagtttat
      841 ggtgaagtcg acagcaacgg tattccgaaa aaaggtctgc tgctggctgc agtgaaaatg
      901 actgccctga tgatcctcat cactctgatg aactctgccg gtggtaaagc atctga
//
LOCUS       DAJVQR010000278          955 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVQR010000278 DAJVQR010000000
VERSION     DAJVQR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            347..640
                     /locus_tag="OQM52_004268"
     CDS             347..640
                     /locus_tag="OQM52_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1565847.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..>955
                     /locus_tag="OQM52_004269"
     CDS             712..>955
                     /locus_tag="OQM52_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1565848.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATL"
BASE COUNT          254 a          201 c          247 g          253 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgcgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgc
//
LOCUS       DAJVQR010000279          916 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVQR010000279 DAJVQR010000000
VERSION     DAJVQR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>916
                     /locus_tag="OQM52_004270"
     CDS             <1..>916
                     /locus_tag="OQM52_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCS1565849.1"
                     /translation="EPCLFGWQALDAGVRVVVLCEGEIDCMSYAQYGISALSVPFGGG
                     KGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSRLGEHRCRLVTLPYKDIN
                     ECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWESLAD
                     KFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVT
                     GIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSETKR
                     HKNGVRIPEITGDFGDHSEPLAWVIGSLL"
BASE COUNT          226 a          196 c          267 g          227 t
ORIGIN      
        1 gcgaaccttg cctgttcgga tggcaggctc tggacgctgg cgtgagggtg gttgtgcttt
       61 gcgaaggcga aattgattgt atgagctatg cgcaatacgg catctcggcg ttatccgtgc
      121 cgtttggtgg cgggaaaggc gctaagcaac agtggattga gtttgagtat cacaacctcg
      181 acaggtttga ggaaatattc atctcgatgg acgttgatga tgttggtcgt gaagccgcaa
      241 gggaaatcgt aagccgactc ggtgaacatc gttgccgtct tgttactctg ccgtacaaag
      301 acatcaacga atgcctgatg aacggtgtta ccgaggatga aatctggcag tacatcggca
      361 cggcatccta cttcgatcct gaagaactct acagtgcgcg agagttttac caggacacta
      421 tcaacgcttt ctacggcaag cagcagtatc tgtttaatcc accgtgggaa tctctggcag
      481 ataaattcca gttccgtgag gcagagttga cgctggtcaa tggtgtgcac ggtcacggaa
      541 aggcatgccc actgaatgag cctattcttt tagctgatgg gacatggact actcacggga
      601 atgtaaaaat tggcgatcag gtggcgtcag tagacggcaa tccgtcaact gtcactggga
      661 tattcccgca gggtgttaga gatgtttacc gagtcacatt tgaagatggt cgttatgttg
      721 attgcgcagg cgatcaccta tgggaggtca ctagtcgtgg attcacgaaa ggcgagaaac
      781 gccgcgtgat agataccttc gggttgaagc ggttgagtga aacgaagagg cacaaaaatg
      841 gcgttaggat tcctgaaata actggtgact ttggcgacca ctcagagcca ttagcatggg
      901 ttatcggctc ccttct
//
LOCUS       DAJVQR010000280          879 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVQR010000280 DAJVQR010000000
VERSION     DAJVQR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(133..798)
                     /locus_tag="OQM52_004271"
     CDS             complement(133..798)
                     /locus_tag="OQM52_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1565850.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          224 a          219 c          198 g          238 t
ORIGIN      
        1 cgcgtaatgt taactggggc ttttctctat ctgccttttg gtgttcatgc ctgaagcaga
       61 tagcctcaag cacccgcagt cattctactt acctcgccaa tatgaaatca atcagaaagg
      121 tgccccataa aatcactcct tctcttcttt accgtagtgg agttgaccaa ttttgataag
      181 agggcgtccc tgagatttgc ggtgtagatt ggtatcgcgc agagaataca cacagccaca
      241 atattcctgc tgatagaatt tttcgcgctt gctgatttca atcatacggg acgagccgcc
      301 ctgcttgcgc cagttataat cccagtacac catgcccgga taatgcgcga cggctcgccg
      361 cccacagtcg ttaacctgct gcatattttt ccagcgtgaa atgcccagtg aactgctgat
      421 cacactgaaa ccattttcag cggcgtacag cgctgtccgc tcaaaacgca tgtcaaaaca
      481 catggtgcaa cggatccctc gttcgggctc ccattccatt cctttggctc gttcaaacca
      541 gttgtctgtg tcgtaatcag catcaataaa cggcacgccg tgttgttcag caaagcgaat
      601 attctcatcc ttacgaatta aatactcttt ctgaggatga atgttcgggt tgtagaaaaa
      661 gatggtgtag tcgatttccg aggcctgaag cgcctccatc acctcaccgg aacatggagc
      721 acagcaagag tgcagtagta gtttgtttgc cccgtttggg agctccaatt tgggccgttt
      781 gaaatcagca actgtcataa atatgtttat tgaggtcata aaaatatcaa agagtgtagc
      841 attagaacgg ggctatcgga aacagatgtg tgactgctc
//
LOCUS       DAJVQR010000281          860 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVQR010000281 DAJVQR010000000
VERSION     DAJVQR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>860
                     /locus_tag="OQM52_004272"
     CDS             <1..>860
                     /locus_tag="OQM52_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS1565851.1"
                     /translation="AAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFSKDA
                     NQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLMGRS
                     EEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAMKKG
                     ALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSENGL
                     QDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTLAYR
                     PLNDTQRKIV"
BASE COUNT          260 a          152 c          208 g          240 t
ORIGIN      
        1 gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc cgcttatcag
       61 cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga tttcgtattt
      121 tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac aacgggttta
      181 gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag agagtctcta
      241 ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg tctgatgggg
      301 cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc tggtaagggt
      361 cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg tatggttcag
      421 gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat gaagaagggg
      481 gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa acaaatcacc
      541 gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg gaacttgaaa
      601 aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg tctgcaagat
      661 tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa gagtcttggt
      721 gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt gaccgatatt
      781 agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc actaaatgac
      841 actcagagga aaattgttga
//
LOCUS       DAJVQR010000282          841 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVQR010000282 DAJVQR010000000
VERSION     DAJVQR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            20..562
                     /locus_tag="OQM52_004273"
     CDS             20..562
                     /locus_tag="OQM52_004273"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565852.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
BASE COUNT          243 a          156 c          206 g          236 t
ORIGIN      
        1 taacaccaaa ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag
       61 aactcagcat gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga
      121 tggtgtgaca gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc
      181 ctccggcggg gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc
      241 cactttgctt attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg
      301 agcaaaaagc ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg
      361 ccgttatctg agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa
      421 cattacagca gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat
      481 ggtgaatgaa gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag
      541 catgtcaggc tggaacctgt agagtcggtc ttcacttttt aggccagtgc gtaagtgtta
      601 gtgtcagctc ctgccatttc agcagaggaa attattctgc tgatgaaagc tttggcatca
      661 ggattcagcc tcaaacgtat gcgtggtcgt cagaggcagt taggtcagaa gcaataattt
      721 tagacagtga gaccatgtgc attggtttta aaaatgccgt ctatgttcat gattgccttg
      781 atttgcatat ggaacaactg gatttagatt attgcggctc aacaggcgtg gttatagaga
      841 a
//
LOCUS       DAJVQR010000283          822 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVQR010000283 DAJVQR010000000
VERSION     DAJVQR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            45..347
                     /locus_tag="OQM52_004274"
     CDS             45..347
                     /locus_tag="OQM52_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1565853.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            338..820
                     /locus_tag="OQM52_004275"
     CDS             338..820
                     /locus_tag="OQM52_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1565854.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
BASE COUNT          206 a          162 c          208 g          246 t
ORIGIN      
        1 gttaccattt gcgataacat taattgcata gcaaattgtt accaatggag gtttttatgt
       61 ctacagctgc aagcgtccgt aaaacaccgc gagaacatca gattaacatc cgggcaactg
      121 atgaggaacg agccgttatt gattatgctg cgagcttggt caataagaac cgtaccgatt
      181 tcatcatgga actggcgtac caggaagcta aaaacattat cctggatcaa cgtttatttg
      241 tgctggataa cgaacgttat gattcgttta ttacgcaact ggaagctcct gtgcagaatg
      301 cagaagggcg tgaacgtctg atggcggtaa agcctgaatg gaaataaatg tcaccgcgcc
      361 agcattgttg acggatgagc atatacttca gccatttgac tgtggaaatg aggtgctaag
      421 taactggtta cgtggtcggg ctatgaaaaa ccagatgctc aatgcttctc gcacgttcgt
      481 tatttgcttg gaggatactt tacgtattgt gggatactac tcattagcta ctggttccgt
      541 cactcatgcc gagcttggtc gcagtttgcg gcataacatg cccaatcctg tccctgttgt
      601 tctgttaggg cgtctggctg ttgatgtttg tacccagggg catggtttcg gtaaatggct
      661 actaagtgat gccattcacc gggttgtcaa tctggctgat caggttggca ttaaggctgt
      721 tatggtacat gcaattgatg atgacgccag agcattttat gagcgttttg gttttgttca
      781 gtcagttgtg gcacctaata ccttgtttta taaagtctga gt
//
LOCUS       DAJVQR010000284          818 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVQR010000284 DAJVQR010000000
VERSION     DAJVQR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            419..>818
                     /locus_tag="OQM52_004276"
     CDS             419..>818
                     /locus_tag="OQM52_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1565855.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETL"
BASE COUNT          294 a          158 c          130 g          236 t
ORIGIN      
        1 aacaaagcca acggtattct gatttctgaa gttaatttca gactcgttta agctccattt
       61 tcaaaaaaaa tatatcaggg tttgttacta ataacttctg aaaacagaaa aaaatagagc
      121 agtaattcta tttcagagag gagtaactcc atctatcctc gatacgtact gaacgttatc
      181 gtgatatatt catcatgctt tcttcaccat tattcgagta tagggagaga cgcaatgacc
      241 ttaaatacct ttttatggct gaattaccat tctcaggaat aacagcagac tccttcccat
      301 agacggctta gggggaaagc aatacccgga gaaagggtac aaagcaaact gttttaaaat
      361 taataagcca tattctattt aactagaatt cttatcgttg ataagaattc tagttataat
      421 gattaaatca accaatatac aggcaatcgg ttctggcatt atgcatcaaa taaacaatgt
      481 atactcgtta actccattat ctttacctat ggaactgact ccatcttgta atgaatttta
      541 tttaaaaacc tggagcgaat gggaaaagaa cggtacccca ggcgagcaac gcaatatcgc
      601 cttcaatagg ctgaaaatat gtttacaaaa tcaagaggca gaattaaatt tatctgagtt
      661 agatttaaaa acattaccag atttaccgcc tcagataaca acactggaaa taagaaaaaa
      721 cctattaaca catctccctg atttaccacc aatgcttaag gtaatacatg ctcaatttaa
      781 tcaactggaa agcttacctg ccttacccga gacgttag
//
LOCUS       DAJVQR010000285          817 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVQR010000285 DAJVQR010000000
VERSION     DAJVQR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            198..695
                     /locus_tag="OQM52_004277"
                     /pseudo
     CDS             198..695
                     /locus_tag="OQM52_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            736..817
                     /locus_tag="OQM52_004278"
                     /pseudo
     CDS             736..817
                     /locus_tag="OQM52_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          213 a          202 c          239 g          163 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccat actcctccgg cagaagcaga aaagcttatt
       61 atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac actctccagg
      121 aaacccgggg cggttcaaag cgccagcccc gtcccgcccg atagagcgcg gcatagcggg
      181 ccccggatta cttgcgtgcg acggcagaag aagccctgaa gcgaatcagt gaactgtacg
      241 ccatcgagga tgaaatacgg ggattaccag agtcagagtg tcttgcagtc aggcagcagc
      301 gaagcaaagc gttactgacg tcgctgcatg aatggatggt ggagaagaat ggcacgctgt
      361 cgaaaaaatc cagactgggc gaagcattca gctatgtact gaatcagtgg gacgccctct
      421 gttattacag tgatgacggt ctggcggagg cggacaataa cacagcggaa agagcgcttc
      481 gtgcagtctg tctcggaaag aaaaattacg tgttcttcgg tagcgatcac ggcggcgagc
      541 gtggtgcact gctgtacggg ctgatcggca cctgccgtct gaacggtatc gatccggaag
      601 cgtatctgcg ccatattctg agcgtactgc cggaatggcc ttccaaccga gttgacgacc
      661 tcctgccatg gaaggtggtg ctaccatcag gataatcgtc aatacggtgt tcggtgaacg
      721 tttacttgga gcatcatgct tcatgaagaa aaactggcac gtcatcaacg taaacaggcg
      781 atgtataccc gaatggcagc cttcccggcg gtgaaaa
//
LOCUS       DAJVQR010000286          810 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVQR010000286 DAJVQR010000000
VERSION     DAJVQR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            11..>810
                     /locus_tag="OQM52_004279"
     CDS             11..>810
                     /locus_tag="OQM52_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS1565856.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT          178 a          213 c          232 g          187 t
ORIGIN      
        1 aggctgaatc atgagagatc tgttacaacg cccggattta ttcagtatca ataccgcgac
       61 actgggctat aaaacgccgc tacccgccat tattgatgcc tgcgccgcac gcggtattgg
      121 cgcaattgcc ccctggcgca gggagttgca gggtgaagat ctccagcaaa tcacccgcca
      181 gcttgccgcc agtaacatga gcgtttccgg tttgtgccgt agcacttact acaccgcgcc
      241 gacactggcg gaacgcaaac tggcgattga tgataaccgc cgggcgctgg acgacgcagc
      301 ggtgcttaac gccgcctgtt atatgcaggt ggttggcggt ctgccaacgg gcactaaaga
      361 tctatatgaa gcgcgagaac aagtgaaaca agggattcgc cagttgctgc cacattcgaa
      421 agatgtcggc gtgccaattg cgctggagcc actgcacccg atgacagctg ccgatcgctc
      481 ttgcctgtgc acgttacgtc aggcgctgga ctggtgtgat gaactcgatc ccgatggtga
      541 gtttggcctc ggcgtggcgg tggatgttta tcacgtctgg tgggatccgg atctcgccag
      601 tcagatcctg cgtgccggaa aacgcatcct cgcgtttcat gtttccgact ggttaatgcc
      661 gaccactgat ctggtcaatg accgtggtat gccgggagat ggcgtcatca atattccgtc
      721 aattcgtcgg ctggttgaaa acgccggatt taatggtgct attgaactgg aaatattctc
      781 gccttactgg tggcagaaag atattaacag
//
LOCUS       DAJVQR010000287          796 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVQR010000287 DAJVQR010000000
VERSION     DAJVQR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..65)
                     /gene="ospE1"
                     /locus_tag="OQM52_004280"
     CDS             complement(<1..65)
                     /gene="ospE1"
                     /locus_tag="OQM52_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCS1565857.1"
                     /translation="MLTQTIFPCLPQKQENIILEVS"
     gene            complement(174..304)
                     /locus_tag="OQM52_004281"
                     /pseudo
     CDS             complement(174..304)
                     /locus_tag="OQM52_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(302..397)
                     /locus_tag="OQM52_004282"
                     /pseudo
     CDS             complement(302..397)
                     /locus_tag="OQM52_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          227 a          141 c          188 g          240 t
ORIGIN      
        1 gatacttcaa ggatgatatt ttcttgtttt tgtggaagac aagggaatat agtttgtgta
       61 agcatattac ttccttatgt tatattttgg taaaaaattt atttttttac attatagtcg
      121 gctaaatgaa gtttactgta aaaaaacgat actttttcgg gtggcgcttc caatcagtag
      181 aatatgtgtt tttcaatgaa ggagccgatg acttttcgtg gatttccacg gaacgcgaga
      241 agcagattgt tttacgtgcc agccgcttga tgcgggtgcg gagggtcagg ttgttacgtt
      301 caagatatag catgtgctgg atgaagatcc cttttctggt cacctgttta tctttcgtgg
      361 ccgacggggt gacactgtta aaatgtaatg aacgcatccc acacaattaa acacggagta
      421 gtatgatggt taaaagggtg aaactggcag gtgtggagcc tcgcagcttt ccggcatcat
      481 actgcccaat acgggatgca taaagcgtcc tcagactgga gactccacaa atgaaatata
      541 caccggttgg cgttgatatg gcaaaacatg tcattcagat tcacttcatc aatgagcaca
      601 tgtcagcgcc agtgatataa gacggtaatg gggatgcgga cgatttcctg gactctcaga
      661 agatgaaaca tgtttactga agaacaaaaa attcgcgcta ttgagcttta tttcaaatat
      721 ggtaagaagc ttgaggtgta ctggcaatag cggacaccac catttgttct ttttctaagc
      781 agtcatctga taatat
//
LOCUS       DAJVQR010000288          784 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVQR010000288 DAJVQR010000000
VERSION     DAJVQR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            96..>784
                     /locus_tag="OQM52_004283"
     CDS             96..>784
                     /locus_tag="OQM52_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1565858.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          191 a          188 c          245 g          160 t
ORIGIN      
        1 cgaaaggtgt tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca
       61 gtgcgatccc gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg
      121 ttctggagcg tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg
      181 cggagtggcg cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc
      241 tgaatcgtca gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc
      301 ggaagtgttc gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc
      361 aggcgaaatc catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta
      421 agcccggcac cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg
      481 ggcgtagcgt gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg
      541 acaacggcaa atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc
      601 tggatgaaat tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg
      661 ttgatcgccg gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag
      721 cgatgaatac acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct
      781 gggt
//
LOCUS       DAJVQR010000289          784 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVQR010000289 DAJVQR010000000
VERSION     DAJVQR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            96..784
                     /locus_tag="OQM52_004284"
                     /pseudo
     CDS             96..784
                     /locus_tag="OQM52_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          192 a          190 c          248 g          154 t
ORIGIN      
        1 cgaaaggtgt tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca
       61 gtgcgatccc gccaggcttc agggggtaac ggaccatgaa aaatattgcg acaggcggcg
      121 ttctggaacg catccgccga ctgaccccgc aacatgtaac cgccccattc agaacggtag
      181 cggagtggcg cgagtggcaa cttgctgaag gccagaaacg tagcgaggag atcaaccgcc
      241 tgaatcgcca gttgcgggtg gaaaaaattc tgaatcgctc aggcatccag ccgttgcacc
      301 gtaaatgctc gtttgcgaat taccaggtgc agaacgacgg ccagcgatac gcgttaagcc
      361 aggcgaaatc catcgccgat gaactgatga ccgggtgtac aaattttgcg ttcagcggaa
      421 aacctgatac cgggtagaat cacttagcgg cagctatcgg gaatcgcctg ctgaaagacg
      481 gtcagacagt gattgtggtt accgtggctg atgttatgag cgccctgcac gccagctatg
      541 acgacgggca gtcaggcgaa aaatttttgc gggagctgtg cgaagtggat ctgctggttc
      601 ttgatgaaat tggcattcag cgcgagacga aaaacgagca ggtggtgctg caccagattg
      661 ttgatcgccg gacagcgtcg atgcgcagcg tggggatgct gacaaacctg aactatgagg
      721 tgatgaaaac attgctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct
      781 gggt
//
LOCUS       DAJVQR010000290          782 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVQR010000290 DAJVQR010000000
VERSION     DAJVQR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            46..234
                     /locus_tag="OQM52_004285"
     CDS             46..234
                     /locus_tag="OQM52_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565859.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            244..>782
                     /locus_tag="OQM52_004286"
     CDS             244..>782
                     /locus_tag="OQM52_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCS1565860.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKL"
BASE COUNT          166 a          223 c          219 g          174 t
ORIGIN      
        1 atatgtatgc tctgaggacc tgttttatta aggactcatc ctcttatgtt gacccgcaaa
       61 agtatagata ccgttctgct ctctgttggt gctgagaagc tctcacaacg tgaatgggac
      121 tggatgaaga tgctcaaacc catggaccca ccacctgcga tggtcgcggc ctccatcctg
      181 gagcgccgtg gcgacacagc agcccttacc cggctacagg ataccggggg ttaatccgtc
      241 ctcatgttgt ccgcttttac tccccgcccg ctgaaacgtc tgttcacggc caaccagtgc
      301 tggacatcct tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa
      361 atgctggcct gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag
      421 tgcccccacg tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga
      481 aaaaaggcca cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg
      541 gtacacctgg tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg
      601 ctgctgaatg acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg
      661 gggcaggaac ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat
      721 ccgcatgtac atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag
      781 ct
//
LOCUS       DAJVQR010000291          780 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVQR010000291 DAJVQR010000000
VERSION     DAJVQR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..264
                     /locus_tag="OQM52_004287"
     CDS             <1..264
                     /locus_tag="OQM52_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1565861.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>780
                     /locus_tag="OQM52_004288"
     CDS             288..>780
                     /locus_tag="OQM52_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1565862.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNPPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSE"
BASE COUNT          208 a          183 c          222 g          167 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat attattctga
      361 aatcccgggt aaaccccccg gaagtggtga tgaacactgt tgctgatgtg atgacaggaa
      421 acgatggtga atactgtttc catgcgcgga ctggaaaata tggtgtgtat ctgaaacagg
      481 actggcgcaa cgagtacaac gttggcgaca ttgctgtata tgaggactca aagcccggca
      541 cgctgaatga ctttctgatt gctcctgatg agggcgacct gaaaccggat gtcgtcaaac
      601 gctttgagga aatggtggcg caggcgcagc agagcgccgg ggccgcagcc ggaaacgcac
      661 agcagacggc gcaggatgtg gcggcagccg caggttatgc ccgcgcagca gagcaggcca
      721 aaaatgacat tgatgctgcg ctgaccggca ctctgaaaac ggctaaccat ctgtctgaaa
//
LOCUS       DAJVQR010000292          769 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVQR010000292 DAJVQR010000000
VERSION     DAJVQR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            63..284
                     /gene="kil"
                     /locus_tag="OQM52_004289"
     CDS             63..284
                     /gene="kil"
                     /locus_tag="OQM52_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1565863.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            278..454
                     /locus_tag="OQM52_004290"
     CDS             278..454
                     /locus_tag="OQM52_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565864.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            529..>769
                     /locus_tag="OQM52_004291"
     CDS             529..>769
                     /locus_tag="OQM52_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565865.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLA"
BASE COUNT          268 a          141 c          155 g          205 t
ORIGIN      
        1 gctaagtatt aaataacttt cctgagattt cctttgtaag actgtttatt aaggaggctt
       61 ccatgattgc tcatcacttc ggaactgatg aaataccacg tcagtgtgtg actcctggcg
      121 attatattct tcatgaaggt cggacatata tcgcctcggc aaacaatatt aaaaagcgaa
      181 aactttatat tcgtagcctg actacaaaaa catgcatttc tgactgcatg attaaagtct
      241 tcctcggtcg tgatggttta cctgtaaagg cggagtcatg gtaatgacta agaaaataaa
      301 atgtgcttat cacctttgca ataaagaaat tgaagaaagc aaaatcatta caagaccact
      361 tcatttcatg cgtggagtta taccaacgac ggaaatgaaa aaatattgta gtgaaatctg
      421 tgccgaaaaa gaccagatgg cacacgaact ttaattaact gactatccga aactgaattt
      481 atgccagcaa tggcagggat tcgctcaacc ttaattaagg agaaaaacat gattaccagt
      541 tatgaagcca ctgttgtaac tactgatgac attgttcacg aggttaatct ggaaggaaag
      601 cgtattggct acgtgattaa gacagaaaat aaagaaaccc cattcactgt ggttgatatc
      661 gacggtccat caggcaacgt taaaacactt aacgatggtg taaaaaaaat gtgtctggtg
      721 cacataggaa agaatctgcc cgcagaaaaa aaagccgaat ttctggcaa
//
LOCUS       DAJVQR010000293          761 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVQR010000293 DAJVQR010000000
VERSION     DAJVQR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..702
                     /gene="lsrF"
                     /locus_tag="OQM52_004292"
     CDS             <1..702
                     /gene="lsrF"
                     /locus_tag="OQM52_004292"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCS1565866.1"
                     /translation="GYFQGPTIGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
BASE COUNT          191 a          158 c          224 g          188 t
ORIGIN      
        1 ggttattttc aggggccgac tatcggactt gaacgcattg atataaatat cgccccgctg
       61 tttgaacatg ccgatgtatt aatgtgtacg cgcggcattt tgcgcagcgt agttcccccg
      121 gcgaccaata agccggtggt actgcgggcg tcaggtgcga actctattct ggcggaatta
      181 agtaatgaag ccgtggcgtt atcgatggat gatgccgtgc gcctgaacag ttgcgcggtg
      241 gcggcgcagg tttatatcgg cagcgaatat gaacatcagt cgatcaaaaa tattattcag
      301 ctggtcgatg ccggaatgaa agtgggaatg ccgaccatgg ccgtgactgg cgtgggcaaa
      361 gatatggtgc gcgatcagcg ttatttctcg ctcgcgactc gaatcgccgc tgaaatgggg
      421 gcgcaaatta tcaaaaccta ttatgtcgaa aaaggttttg aacggattgt tgccggatgt
      481 ccggtaccca ttgttattgc tggcggtaaa aaattaccgg agcgcgagac gctggaaatg
      541 tgctggcagg ctatcgatca gggcgcttct ggtgtggata tggggcgtaa tattttccag
      601 tctgaccatc cggtggcgat gatgaaagcc gtacaggcgg tggttcacca taacgaaacg
      661 gctgatcggg catatgaact ctatctgagt gaaaaacagt aactgcggat ttaaggagaa
      721 gaattatgca cgtcacactg gttgaaatta acgttcatga a
//
LOCUS       DAJVQR010000294          761 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVQR010000294 DAJVQR010000000
VERSION     DAJVQR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            11..163
                     /locus_tag="OQM52_004293"
     CDS             11..163
                     /locus_tag="OQM52_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1565867.1"
                     /translation="MEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTM
                     SQQEVK"
     gene            163..513
                     /locus_tag="OQM52_004294"
     CDS             163..513
                     /locus_tag="OQM52_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1565868.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          250 a          165 c          187 g          159 t
ORIGIN      
        1 tgaattattc atggaaaagc gttttggtga cttagtagat cgtcggagag caaaaaacgg
       61 cgataacgaa tacatggcat gggatatgac tctcggttgg atcgtctggc agcaacgagc
      121 tggtatccat ttctcaacaa tgtcacagca agaggtgaaa taatggagcc atacagcctc
      181 acactcgatg aggcctgtca ttttctcaag atatccagac cgactgccat taactggata
      241 cgcacagggc gtcttcaggc aacacgcaaa gatcccacta agaataaatc tccttacctc
      301 acaacacgac aagcctgcat tgcggctctt cagtctccgc tgcatactgt ccaggtgagc
      361 gcgggtgatg gcataacaga ggaaagaaaa tgtcactctt ccgcagaggt gaaatatggt
      421 acgccagttt cacattgccg aacggtaaaa gatttaaaca gtctcttgga acaaaggaca
      481 aaaggcaggc gacagaactc catgacaagc taaaggctga agcatggcgg gtcagcaaac
      541 ttggtgaaat acctgatata acgttcgagg aagcgtgtgt caggtggctt gaagagaaag
      601 cacataaaaa atcactggac gatgacaaaa gccggatcgg attctggctt caacatttcg
      661 caggaatgca actaagagac attactgaat caaaaattta ttcagcaatg cagaaaatga
      721 cgaaccggcg tcatgaggaa aactggagac tcagggcaga a
//
LOCUS       DAJVQR010000295          754 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVQR010000295 DAJVQR010000000
VERSION     DAJVQR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..374)
                     /locus_tag="OQM52_004295"
     CDS             complement(<1..374)
                     /locus_tag="OQM52_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565869.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSV"
     gene            complement(371..754)
                     /locus_tag="OQM52_004296"
     CDS             complement(371..754)
                     /locus_tag="OQM52_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565870.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          174 a          223 c          182 g          175 t
ORIGIN      
        1 acagacttcg tacataccgc tctgcaataa atccccgtac cacaggcctc acagaacttc
       61 ttcccatgaa aaaactgctc acacatcaca caatgccact ccagcacgac cggtaatggc
      121 gataaaaaca tcgccatatc ctcaatgtaa gggtgggact tctccggatt cagcaccaca
      181 cagtccgcct tctgttgctc actcagggca tgtaaatcat gctcaaacca cgccccctga
      241 gcatctgtct gcagaatcaa ccggccatgg caggaaaggc agaaacactg ccggatattt
      301 ctgctgagcg cagagtcggc actgataatg tgctcacccg gcgtgatccc cagccccgtt
      361 tttataccgt tcattctgct cccccctcct cactgaagcg cccggtctgg cagtgtgtgc
      421 agtactgctc cccataataa tcgtggtgac attgcctgca gtgccagctg gctttacgta
      481 ctacgggtaa ggtatccggg acgaattgct gcagacgttt aatcaaccgt atttctctgc
      541 gttccggcct gacataaggg cactcatgac cgtgctccgt cagcccttcg tcagcgtgtt
      601 caaaccaggg ccgttcagtg tcgtattgcg ggtggtatct gagtgcactg ccgcaaaggt
      661 ggcaggtgta gctatcgtaa ggtgcagtct gtgcggtacg ggcaccggtc agacgtccgt
      721 tgccatcaag tgcgagaaaa gattttgcgt acat
//
LOCUS       DAJVQR010000296          752 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVQR010000296 DAJVQR010000000
VERSION     DAJVQR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..645
                     /gene="ospC2"
                     /locus_tag="OQM52_004297"
     CDS             <1..645
                     /gene="ospC2"
                     /locus_tag="OQM52_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1565871.1"
                     /translation="TDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVS
                     LDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQ
                     ALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKV
                     LEYFISNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          274 a           98 c          147 g          233 t
ORIGIN      
        1 actgatgtgc caatatttaa tactaaagat atgaggcttg gaattgggtt gcatcttatt
       61 gattttataa gaaagagtaa agatcaaaga ttccgagagt tttgttataa taaaaacatt
      121 gatcctgtta gtctggatag aattataaac tttgtgtttc agcttgagta tcatatacca
      181 agaatgctaa gcacagataa cttcaaaaag ataagactca gagatatatc tttggaggat
      241 gccattaaag catccaatta tgaagaaatt aacaataagg tgactgataa aaaaatggct
      301 catcaagctc ttgcttattc tctaggcaat gcaaaatctg atatggcact ttatttacta
      361 tctaaattta attttacaaa acaagatatc gcagagatgg agaaaatgaa caacaatatg
      421 tattgtgagc tgtatgatgt tgagtattta cttagtgaag atagtgccaa ctataaagtg
      481 ctagaatatt ttattagtaa tggattggtt gatgtaaaca aaagatttca aaaagcaaat
      541 agtggggaca ctatgcttga caatgcaatg aaaagcaaag attcaaaaac aattgatttt
      601 ttattaaaaa atggagcggt atcaggcaaa cgatttggga ggtgacctaa aatatattta
      661 ggttgtgccc tgaatctgac gttgtcattt ttgttatatt gatcgatata ggggatggtg
      721 cagaccgttc ccctgcaaca acacattgca tg
//
LOCUS       DAJVQR010000297          741 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVQR010000297 DAJVQR010000000
VERSION     DAJVQR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          282 a          104 c           92 g          263 t
ORIGIN      
        1 attatcatgt attaaataga atgttggctt cccattttta ctgtaataag ttctgcctat
       61 ttccgtataa tcataatttt cttcttcatc accattctga cacaacccga atcctctatc
      121 tggatcgaac tcaaccatat tcattgtgtc taacattagg ttgtaaactg tctttaaagt
      181 atttgtattc ataacatatc tccttttgtt tttctgttat atataattaa taacactttt
      241 aaaataatag tcaaataaat atatactttt attttaacta tccgataagc ggtataagta
      301 aaacactcta tatttaaatg attattattt taactggaag gataatagaa aagaaaaaag
      361 ccccggtgag agggctttag tcatacacac acatacatag aaaggtgaag catcataaac
      421 ataaaaactt ttatataaag gacaagattg cttgtctctt atatattaat tatcacttat
      481 tatatataag tcaactttaa ttgagaaaat atttcaaaaa agtagcatag gttatccgtc
      541 cgtcctgttt tattaaaata cattaccttt aatatttaca attgggttag atatgttatc
      601 tatttaatat acacaataga aaaggaaaag gtgattaaca tgagtattat tttaaacaaa
      661 gtaaaagcat ttacaaacac aaaagaaaac agaattgaaa aacttttatt cagcaatgat
      721 attgatttgg tgaaaagagt a
//
LOCUS       DAJVQR010000298          737 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVQR010000298 DAJVQR010000000
VERSION     DAJVQR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            125..433
                     /locus_tag="OQM52_004298"
     CDS             125..433
                     /locus_tag="OQM52_004298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565872.1"
                     /translation="MRKTKTLFEQLKDRANQLSAGEAIIVLDEINKKEGFENAVIFLN
                     SRMKHIREAILKDTFTLQGCRNVNHELASELIARVQQEQLSDTIQATTNNEATTRKRL
                     "
     gene            complement(486..722)
                     /locus_tag="OQM52_004299"
     CDS             complement(486..722)
                     /locus_tag="OQM52_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565873.1"
                     /translation="MKLIIDTDLNEYEDENGVNHPAIWVYEKGEDCEPVVVLKATEQP
                     EIWKIGNVYSALTHNALLSETELKALVKAGKVMK"
BASE COUNT          229 a          144 c          145 g          219 t
ORIGIN      
        1 aataacaatg aaacactaat atttacagcc ccttcggggg cttttaatat tttagtatat
       61 ttatttttga tttttatttt aaatgtgtta ttaattggac tggtaaataa ataaaggtga
      121 ttatatgaga aaaacaaaaa cattgtttga gcaattaaag gatagagcta atcagctttc
      181 agcgggtgaa gcaataatag tgcttgacga gatcaacaaa aaagaagggt ttgagaatgc
      241 tgttatcttt ctcaacagta gaatgaaaca tatcagggag gcaattctta aagacacgtt
      301 caccctacag gggtgtagga acgtcaatca tgagctggca agcgagctta tcgcaagagt
      361 tcagcaggaa cagctttcag acaccataca ggccacaacc aataatgaag ctactaccag
      421 aaagcgtctg taagactgga agagaagagg acgggtttaa tgttctcttc tctctggttg
      481 ttcactcact tcattacctt tccagctttc accaacgctt tgagttcagt ctctgataac
      541 agcgcattgt gagtaagagc tgagtatacg tttccaatct tccagatctc cggctgttca
      601 gtagctttaa gcaccaccac aggctcacag tcttcacctt tttcataaac ccatatagca
      661 ggatggttaa cgccgttctc gtcctcatac tcatttaaat cagtatcaat gatcagtttc
      721 atagtggttt ccggttt
//
LOCUS       DAJVQR010000299          714 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVQR010000299 DAJVQR010000000
VERSION     DAJVQR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>714
                     /locus_tag="OQM52_004300"
     CDS             <1..>714
                     /locus_tag="OQM52_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCS1565874.1"
                     /translation="ERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRV
                     FTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVK
                     MVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSL
                     AGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTR
                     KGKVCHLLTSMTDAMRFPGG"
BASE COUNT          181 a          207 c          203 g          123 t
ORIGIN      
        1 gagcgtaaag aacctcttca ccagattgtg aatcgcctgg acatcatgct gccgggcaat
       61 cgccccttcg ttgcccccag tgccgttatt caggcccgcc agcgcctggg aagtgaggct
      121 gtccgccgcg tgttcacgaa aacagcgcag ctctggcata acgccacgcc gcatccgcac
      181 tggtgcggcc tgaccctgct ggccatcgat ggtgtgttct ggcgcacacc ggatacacca
      241 gagaacgatg cagccttccc ccgccagaca catgccggga acccggcgct ctacccgcag
      301 gtcaaaatgg tctgccagat ggaactgacc agccatctgc tgacggctgc agccttcggc
      361 acgatgaaga acagcgaaaa tgagcttgct gagcaactta tagaacaaac cggcgataac
      421 actctgacgt taatggataa aggttattac tcactgggac tgttaaatgg ctggagcctg
      481 gcgggagaac accgccactg gatgatacct ctcagaaagg gagcgcaata tgaagagctc
      541 agaaaactgg gtaaaggcga tcatctggtg aagctgaaaa ccagcccgca ggcacgaaaa
      601 aagtggccgg gactgggaaa tgaagtgaca gcccgcctgc tgaccgtgac gcgcaaagga
      661 aaagtctgcc atctgctgac gtcgatgacg gacgccatgc gcttccccgg agga
//
LOCUS       DAJVQR010000300          712 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVQR010000300 DAJVQR010000000
VERSION     DAJVQR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>712
                     /locus_tag="OQM52_004301"
     CDS             <1..>712
                     /locus_tag="OQM52_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565875.1"
                     /translation="EAKTDARNAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDD
                     DLAAQTTQASNRIRGLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKL
                     AAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVA
                     LEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRS
                     GSSIRGEHPSRRGNKALKR"
BASE COUNT          146 a          241 c          187 g          138 t
ORIGIN      
        1 tgaagctaaa actgatgctc gtaacgctgc catcattgcc gaagctgccc gtaccctgcc
       61 tcacgcgcta cgcacgctga aactggctga cgagcaaatc gccgaactct ccatgctctg
      121 cggcttcgat gatgatcttg ccgcacagac aacgcaggcc agcaaccgta tccgcggcct
      181 tctgacccag atacatccgg caccggagcg cgttctcggt ccgagacttg agcacccggc
      241 ggtactcgat cttctccagc gatatccctc accagaaaaa ctcgcttcgc tgggtgagaa
      301 gaagctggca gcccagctct gcaaacttgc gcctcgtctg ggtaaacgcc ttgcagcaga
      361 catagctcag gcactggccg aacaaaccgt cgtcgttccc ggcacgaatg ccgctgccgt
      421 agtactgcca cgtctggcac tccagctcat cacgctgcgt aagcaaagag acgaggtggc
      481 gcttgaggta gaacagcgag ttcttgctca ccctctttac ccggtcctga ccagtatgcc
      541 cggagtcggt gtcaggaccg cagccagact cctcaccgag gtcgcctgcc gcgccttcgc
      601 ctctgccgca catctcgctg cttatgctgg ccttgcgccg gtaactcggc gatccggctc
      661 gtcaatacgc ggtgagcatc cctcgcgacg gggtaataaa gctctcaaac gg
//
LOCUS       DAJVQR010000301          709 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVQR010000301 DAJVQR010000000
VERSION     DAJVQR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..511
                     /locus_tag="OQM52_004302"
     CDS             <1..511
                     /locus_tag="OQM52_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1565876.1"
                     /translation="NGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVT
                     YTDAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPAD
                     KMIPGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNM
                     TVEQLLSLK"
BASE COUNT          206 a          173 c          155 g          175 t
ORIGIN      
        1 aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga aaatgcacaa
       61 aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc ggcttaattt
      121 taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc aaatcaccaa
      181 aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg cattcaagga
      241 agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata acaccccacc
      301 tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg acaaattcgg
      361 caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc tggagacttt
      421 ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa ttaaaaatat
      481 gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg aaccctatgc
      541 ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga atcaaaaatt
      601 tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta attccctgtg
      661 cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgc
//
LOCUS       DAJVQR010000302          693 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVQR010000302 DAJVQR010000000
VERSION     DAJVQR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(209..>693)
                     /locus_tag="OQM52_004303"
     CDS             complement(209..>693)
                     /locus_tag="OQM52_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1565877.1"
                     /translation="QAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAA
                     REARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWS
                     DADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE
                     "
BASE COUNT          136 a          224 c          163 g          170 t
ORIGIN      
        1 aaaacggctc acaactgcac cattcataat cacatcgcat aaaccacaga ccggactgac
       61 tccggaaaaa cggaggcccg cccccgggcc tcccctgatt catccgtttc cctgttcagc
      121 ctgacagcac gccccggctg ccggatgaca gactccgctt cggtaagcaa agcggtcttc
      181 tgtgattccg ccagttgcgg cttattcatt actcaacgtc aaacgcccga attgaagcca
      241 aatcatccag accgctcagc tcctctttca tttcacgctg acggcgataa atctcatcgt
      301 tgcgatcgac ctgcgcctgc accattgctg ccgccagttc ttccagttcc ggcatcgaca
      361 gtttcacctg ctgattatcg gcatcgctcc acgccatatg tgtttgtgct gtgacagatt
      421 ttgccagcat gactaccggg gacaggcggc ccagtgagtc ggggccagca ttccagatac
      481 gaccgttcca ttcaaacgtg aacggcttcg cctcctgttc tgtgcgccat gcttcaattt
      541 cctgacgtct ggcctctctg gccgcttcca gcatttctgg tgtcacagtg aatggggcta
      601 tctcacccca tttgccactt tgcagttcct gccagatttg ctgacccgtc ggtgcgacat
      661 catcagcggt ggctgtgtag gggactgcct ggt
//
LOCUS       DAJVQR010000303          688 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVQR010000303 DAJVQR010000000
VERSION     DAJVQR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>688
                     /gene="yeaG"
                     /locus_tag="OQM52_004304"
     CDS             <1..>688
                     /gene="yeaG"
                     /locus_tag="OQM52_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1565878.1"
                     /translation="IAKTEPGDENNQDISALVGKVDIRKLEHYAQNDPDAYGYSGALC
                     RANQGIMEFVEMFKAPIKVLHPLLTATQEGNYNGTEGISALPFNGIILAHSNKSEWVT
                     FRNNKNNEAFLDRVYIVKVPYCLRISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFS
                     ILSRLKEPENSSIYSKMRVYDGESLKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFK
                     ILSRVFNFDHV"
BASE COUNT          184 a          181 c          165 g          158 t
ORIGIN      
        1 atcgccaaaa cggaacccgg tgatgagaac aaccaggaca tctccgcgct ggttgggaaa
       61 gtcgatattc gtaaactcga acactacgcg cagaatgacc cggacgccta cggctattcc
      121 ggtgcgctgt gccgcgccaa tcaggggatc atggaattcg ttgagatgtt taaagcaccg
      181 ataaaagtgc tgcatccctt gttaaccgcc actcaggaag gtaactacaa cgggacggaa
      241 ggtatctccg ccctgccgtt caacgggatt attctcgccc actcgaacaa gtccgaatgg
      301 gtcactttcc gtaataacaa aaacaacgaa gccttcctcg accgtgttta catcgtgaag
      361 gtgccgtatt gcttgcgcat ttccgaagag atcaaaatct acgagaaatt gcttaatcac
      421 agtgaattga ctcacgcccc atgcgcccct ggcacgctcg aaacactgtc acgtttttcc
      481 attctttcgc gcctgaaaga gccagaaaac tccagcattt attcaaagat gcgggtttat
      541 gatggcgaaa gtctgaaaga caccgatccg aaagccaagt cgtatcagga atatcgtgac
      601 tacgccggtg tcgatgaagg gatgaacggt ctgtcgacgc gttttgcgtt taagatcctc
      661 tcccgcgtgt tcaacttcga tcatgtag
//
LOCUS       DAJVQR010000304          677 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVQR010000304 DAJVQR010000000
VERSION     DAJVQR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..677
                     /locus_tag="OQM52_004305"
                     /pseudo
     CDS             1..677
                     /locus_tag="OQM52_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          168 a          177 c          163 g          169 t
ORIGIN      
        1 caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg aagagtttta
       61 ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac ccgaatggca
      121 gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga taaccgctcc
      181 tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac gtgagtttgc
      241 cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg cggatgagtc
      301 ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg acgataaacc
      361 atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt acgactggaa
      421 agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc cactgtataa
      481 cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac cttttggccc
      541 ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct gggctgctat
      601 gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg acaatcattt
      661 ctatgggcat cggaaaa
//
LOCUS       DAJVQR010000305          667 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVQR010000305 DAJVQR010000000
VERSION     DAJVQR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            95..334
                     /gene="relB"
                     /locus_tag="OQM52_004306"
     CDS             95..334
                     /gene="relB"
                     /locus_tag="OQM52_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCS1565879.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            334..621
                     /gene="relE"
                     /locus_tag="OQM52_004307"
     CDS             334..621
                     /gene="relE"
                     /locus_tag="OQM52_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCS1565880.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          178 a          137 c          171 g          181 t
ORIGIN      
        1 ttacgactac tttctgactt ccttcgtgac ttgccctaag catgttgtag tgcgatactt
       61 gtaatgacat ttgtaattac aagaggtgta agacatgggt agcattaacc tgcgtattga
      121 cgatgaactt aaagcgcgtt cttacgccgc gcttgaaaaa atgggtgtaa ctccttctga
      181 agcgcttcgt ctcatgctcg agtatatcgc tgacaatgaa cgcttgccgt tcaaacagac
      241 actcctgagt gatgaagatg ctgaacttgt ggagatagtg aaagaacggc ttcgtaatcc
      301 taagccagta cgtgtgacgc tggatgaact ctgatggcgt attttctgga ttttgacgag
      361 cgggcactaa aggaatggcg aaagctgggc tcgacggtac gtgaacagtt gaaaaagaag
      421 ctggttgaag tacttgagtc accccggatt gaagcaaaca agctccgtgg tatgcctgat
      481 tgttacaaga ttaagctccg gtcttcaggc tatcgccttg tataccaggt tatagacgag
      541 aaagttgtcg ttttcgtgat ttctgttggg aaaagagaac gctcggaagt atatagcgag
      601 gcggtcaaac gcattctctg aaccaaagca tgacatctct gtttcgcacc gaaggtgaca
      661 cttctgc
//
LOCUS       DAJVQR010000306          659 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVQR010000306 DAJVQR010000000
VERSION     DAJVQR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            103..>659
                     /locus_tag="OQM52_004308"
     CDS             103..>659
                     /locus_tag="OQM52_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1565881.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAJVQR010000307          656 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVQR010000307 DAJVQR010000000
VERSION     DAJVQR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            72..>656
                     /locus_tag="OQM52_004309"
     CDS             72..>656
                     /locus_tag="OQM52_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCS1565882.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          138 a          177 c          196 g          145 t
ORIGIN      
        1 agcgccggcg gtatgtactg cccggagtgt ggcacggttc actggccgga cggagtcgcc
       61 ccgcccttct gatgctcccc cgttttgccg atatttttca gcagggaaac cgctggctta
      121 actggctgga gaaacaaccg gaaggggcag tccgtccggt ggtaattgaa tcagtgacaa
      181 aaatcatggc ctgcgggacc acactgatgg gctacacgca gtggtgttgt tcatcaccgg
      241 actgttgcca tacaaaaaag gtctgcttcc ggtgtaaaag ccgctcctgc ccgcactgcg
      301 gggtgaaggc tggcgcacag tggatacagt atctgctgag cctggtcccc tactgcccgt
      361 ggcagcatat tgtgttcaca cttccctgcc agtactggtc cctggtgttc cacaaccggt
      421 ggttactggc agagatgagc cgcattgcag cggatgtgat actggaaatc tgccatcagg
      481 cagatgtgga gccggggata ttcacggtga tccacacatg ggggcgtgac cagcagtggc
      541 atccgcatat ccatttatcg acaactgccg gtggtgtgac gtcgggccac acctggaaaa
      601 atcttcattt ttacgcccgt aaggtgatga gcatgtggcg ctaccggata acacgg
//
LOCUS       DAJVQR010000308          649 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVQR010000308 DAJVQR010000000
VERSION     DAJVQR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(24..>649)
                     /locus_tag="OQM52_004310"
     rRNA            complement(24..>649)
                     /locus_tag="OQM52_004310"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAJVQR010000309          644 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVQR010000309 DAJVQR010000000
VERSION     DAJVQR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..149
                     /locus_tag="OQM52_004311"
     CDS             <1..149
                     /locus_tag="OQM52_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1565883.1"
                     /translation="KRLAGDPGRESAPDASSVIHATGGDRVATSQTDRTAWHPDITRD
                     KTRE"
     gene            146..>644
                     /locus_tag="OQM52_004312"
     CDS             146..>644
                     /locus_tag="OQM52_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565884.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPEFPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTA"
BASE COUNT          189 a          168 c          169 g          118 t
ORIGIN      
        1 ccaaacgtct ggccggtgac ccgggaaggg aaagtgcacc tgacgccagc tcagttatcc
       61 atgctactgg aggggatcgc gtggcaacat cccaaacgga cagaacggcc tggcatccgg
      121 atataacccg tgataaaaca agggaatgaa caatgaactc cccgatgata ttgagctgct
      181 taaagccatg ttgcgtaagc aacagagtcg gcttcgacag tatgcctgtc aggtcgcggg
      241 ctatgagcag gaaattgaac ggctgaaagc gcaactcgac aggttgcgtc gtatgttgtt
      301 cggccagagt tcagagaaaa agcgtcataa gcttgaaaat cagatccgac aggcagaaaa
      361 acgactgtcg gaactggaaa accggctgaa cacagccaga aatcttctgg aagatgcatc
      421 gtcagtcaca gattcacctg acaccagtcc cccgtcagaa aacccgatcg ccagtaagcc
      481 tgaattcccg ggacgaaaat cttcacgaaa accgctgccg gcagaacttc cccgggagac
      541 acatcgcctt ctgcctgctg aaaccagttg cccggcctgt ggaggtgttc tgaaagaaat
      601 gggggaaaca atctcagagc aactggatat cattaatacc gcct
//
LOCUS       DAJVQR010000310          641 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVQR010000310 DAJVQR010000000
VERSION     DAJVQR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            74..613
                     /locus_tag="OQM52_004313"
     CDS             74..613
                     /locus_tag="OQM52_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1565885.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
BASE COUNT          267 a          116 c          110 g          148 t
ORIGIN      
        1 aagtcatcgt cgtttattcc agaaagacgg aataggattg agccaaacag gaaaagcaac
       61 acaacttgtc attatgtcgc atggtggatg gaaagagatc tcccatccac aaacactatt
      121 cacgcgtcaa aaaggcgatg gatggactgt agtccccaaa aacctaagaa ttgattttta
      181 tacaaaagac aacgacttca caaagggact aagcgttctt tcagaggtaa acaaacgcca
      241 tactgaagct caaaaagggt taacaccatc actaaacata agcaaagatg acctaaaagt
      301 tctagcgaac gcgcgcaata tacctcaatc aaaattagaa gaagaaatga tgtcacaagc
      361 catatataga aaagaatacg caacaagtaa tgagaagata aaaaactatg ctctatatta
      421 ccatgaacaa gcccaaaata ttatacaaag acatcaagat ggcttaggaa acaaaaatat
      481 tgatatagca tttataactg ataaaaatca taagaagcac ctttctgaca tatttaaagt
      541 aataaatgaa tcaggagtaa aatatgatgt aattcacttt ggcgcttgcc gagtaaatag
      601 agaggagaaa taatcatcat ttaccataat cccttaattg t
//
LOCUS       DAJVQR010000311          638 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVQR010000311 DAJVQR010000000
VERSION     DAJVQR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          201 a          105 c          150 g          182 t
ORIGIN      
        1 agaacaggat actgtgttac gttgtcacta gcaagtatat attaatatat attagtatat
       61 attaggttaa atcacattca acaaaaggtc aggtaggaca ggcggcagca ggggcaggtc
      121 tggatcaggc cggatgttat ggggtaggat tggtcagggt agggctaggg ttagtattgg
      181 gattttaggt tttgtttttc tcaatcctag tttttccagc atcgaggcca gcgaacagga
      241 gaggcggtag cctcatctgt gagtattcca gagcatggaa gcaaaaaaac agcgtttagg
      301 aatgctttta taacgtaact cactgttata tatagatatt ccgtgttatc caaagattcc
      361 agcaaaaaca gacacctcgc cacgtataaa gggagatcag gcagtggaag aagtattcca
      421 gcgtgtggaa tgttattttt ggtctggtgg aataaaaaaa gctcgcaaac ccttgatata
      481 taaggctttt attgaatatt ccatatattc caaacattcc aacaaaacag acgacacact
      541 acagttacta tatgcataat tacgataatt cgagttagga ttaggattta ggcgcgtagg
      601 ttaggttagg cgcaataacc cttatattct aacattag
//
LOCUS       DAJVQR010000312          619 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVQR010000312 DAJVQR010000000
VERSION     DAJVQR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>619
                     /gene="ipaH3"
                     /locus_tag="OQM52_004314"
     CDS             <1..>619
                     /gene="ipaH3"
                     /locus_tag="OQM52_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCS1565886.1"
                     /translation="TISGTYADYFSAWDKWEKQALPGENRNEAVSLLKECLINQFSEL
                     QLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASLEYLDACDNRLSTLPELPASL
                     KHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFLPELPE
                     SLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENRITHIPENI"
BASE COUNT          189 a          159 c          108 g          163 t
ORIGIN      
        1 actatttccg gtacatatgc tgattacttt tcagcatggg ataaatggga aaaacaagcg
       61 ctccccggtg aaaatcggaa tgaagcggtc tccctactta aagaatgtct catcaatcag
      121 ttcagtgagc ttcaactgaa tcgtttaaat ctgtcctcgc tacctgacaa cttaccacct
      181 caaatcactg ttctggaaat tactcagaat gccctaatat cattaccaga attgccagca
      241 tcgctggaat accttgacgc ctgtgacaat cgcctgtcaa cacttcctga attacccgca
      301 tctctgaaac atcttgatgt agataacaac caactaacca tgcttcctga attgcctgca
      361 ttgctggaat atattaatgc agataacaat cagctaacca tgcttcctga attacctaca
      421 tcgctggaag tgctctcagt aagaaataac cagctgacat ttctccctga gttacctgaa
      481 tcactggaag cgctcgatgt aagtactaat cttctggaaa gcctaccagc cgtacctgta
      541 agaaatcatc actcagagga aaccgagata tttttccggt gccgcgagaa tcgcatcaca
      601 cacattccgg aaaatatac
//
LOCUS       DAJVQR010000313          615 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVQR010000313 DAJVQR010000000
VERSION     DAJVQR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            311..>615
                     /locus_tag="OQM52_004315"
     CDS             311..>615
                     /locus_tag="OQM52_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1565887.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          211 a          121 c          100 g          183 t
ORIGIN      
        1 tttttatgta ataattttat aaatgcgttc aaaataataa caaagtacta atagtgatat
       61 tttaatacct gatttttacg tgataattca ggaggcacag aaagcgcata aaaataacaa
      121 cataaaacac cttgccacca cccaagaatt ctctattaca ttgtttttaa tgaaaaaata
      181 tcattccaac aatatctcac gataaataac attaggattt tgttatttaa acacgagtcc
      241 tttgcacttg cttactttat cgataaatcc tactttttta atgcgatcca atcattttaa
      301 ggagtttaaa atggataaga agcaagtaac ggatttaagg tcggaactac tcgattcacg
      361 ttttggtgcg aagtctattt ccactatcgc agaatcaaaa cgttttccgc tgcacgaaat
      421 gcgcgacgat gtcgcatttc agattatcaa tgatgaatta tatcttgatg gcaacgctcg
      481 tcagaacctg gccactttct gccagacctg ggacgacgaa aacgtccaca agttgatgga
      541 tttatccatt aacaaaaact ggatcgacaa agaagaatat ccgcaatccg cagccatcga
      601 cctgcgttgc gtaaa
//
LOCUS       DAJVQR010000314          612 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVQR010000314 DAJVQR010000000
VERSION     DAJVQR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            142..510
                     /locus_tag="OQM52_004316"
     CDS             142..510
                     /locus_tag="OQM52_004316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565888.1"
                     /translation="MDYAQSKEISLHIISSMKLSVHFNKFEVDNEKDLDGIKYIIERN
                     KANIKISTAKIKVVEPGSLLFLSSKMRDTLKATDLFIIYTDVDESNDEEVTKKLRDFA
                     FRHKAFVVQITKSRGGYDNR"
BASE COUNT          249 a           70 c          100 g          193 t
ORIGIN      
        1 aagaactaat agagttaact tctcttatca ttaaagatta aatctgtaag taatccgctt
       61 atctaaaaca tcttccgatt atttgacaat tgggttagtt ttgttattta tatttatata
      121 tgacaaacaa aggagatatt tatggattac gcacaaagca aagagatttc attacacatt
      181 ataagttcta tgaagttgag tgttcacttt aacaaatttg aagttgataa cgaaaaagat
      241 ttggatggta ttaaatatat aatcgaaaga aacaaagcaa acataaagat cagcacagca
      301 aaaattaaag ttgttgagcc gggaagttta ttattcctat caagtaaaat gagggatacg
      361 ttaaaagcta ctgacttatt cattatttat actgatgttg atgaaagtaa tgatgaagag
      421 gtaacaaaaa aactaagaga cttcgctttt agacataaag catttgttgt tcagataact
      481 aaaagcagag gtggttatga taatagataa aatacagtat tgtgattatg actacacgtt
      541 aaaacagatt aaacatggaa actactatca ttttaggatt tataaaaaac aatcaaatca
      601 taaagtagtt ag
//
LOCUS       DAJVQR010000315          611 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVQR010000315 DAJVQR010000000
VERSION     DAJVQR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          190 a          146 c          118 g          157 t
ORIGIN      
        1 aatcaccttt accaatattg tatgaaaaaa cacgcacgac taattgacaa taattttcat
       61 ttccattaaa aaagatcgcg tagcattctt aattcatgag gaactttacc cgccagcaat
      121 ctgagtagcg aaagctgttc atccccaatg tttttgctgg cgggtccttt tttctacggt
      181 cctcctcccc ggaggggggc atttcattat ttttctaatc gttactggtg agaaggcgga
      241 agaaaaagcc agttcttcgc ccctaaataa cagcatgatt ccagtccatg caaaatctga
      301 tttccagctc acacataccg agcgctgtac aaaaaaccgc cagccaaggc agttaagagt
      361 gtgttcccgg ggtttgctta gaatattttg taagttgtcc gaagagatat ttacaacacc
      421 agaatgatga atcatcagtc ccctgccaga ggttcactac gtaacctgac cgacaatgca
      481 ccattctggt gctgaaaaaa gagcactgga actgcaacaa aacaacatca cagaacagga
      541 gatggtaagg agtcagacat tcccacacaa tattgtgtca gtgcaaataa caaccttcgt
      601 ctcaaatctc g
//
LOCUS       DAJVQR010000316          605 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVQR010000316 DAJVQR010000000
VERSION     DAJVQR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..466
                     /locus_tag="OQM52_004317"
     CDS             <1..466
                     /locus_tag="OQM52_004317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565889.1"
                     /translation="IDIITREEIVYCDPGCYLISRSLLNTLIESNVDCVNIIKHEDLK
                     IKFSVKHNMEHPNKTIPKWYRLIPFNKGEERKEIYLNEKNDLIVNERILNLLKRHRVK
                     RLKIEEYEDEHEAKIIEEEKAKPVFITDKNNTMKQIIIFIVIMAVVAYWFFN"
BASE COUNT          276 a           59 c          110 g          160 t
ORIGIN      
        1 tattgatata ataacaagag aagagatagt gtattgcgat ccgggctgtt atttgattag
       61 tagatcgcta ttaaacacat taatagaaag taatgttgat tgcgttaaca tcattaagca
      121 tgaagattta aaaataaagt ttagtgttaa acacaatatg gaacatccaa ataaaacaat
      181 acccaaatgg tatagactga taccatttaa caaaggagaa gaaagaaaag aaatatattt
      241 gaacgaaaaa aatgatctta tcgtaaatga aagaatactt aatctattaa aaagacatag
      301 agttaaaagg ttaaaaatag aagagtatga agatgaacat gaagcaaaga taatagaaga
      361 agaaaaggct aaaccagtat tcataacaga taaaaataac acaatgaagc aaatcatcat
      421 ttttattgtg ataatggctg ttgtcgctta ttggttcttt aactaaatgg agaaaacaat
      481 gggtgaaacg aactggttag aaatagagag taaaaatcat ataaaagtga ttaatgatac
      541 aaagataaat aaacaggatg aaaagatagc tatacgtgtt gagcgtagtt tattacgaaa
      601 gataa
//
LOCUS       DAJVQR010000317          603 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVQR010000317 DAJVQR010000000
VERSION     DAJVQR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            290..>603
                     /locus_tag="OQM52_004318"
     CDS             290..>603
                     /locus_tag="OQM52_004318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565890.1"
                     /translation="MKDKIIRILKEYSDDDSKRGKLLKDFSMFVIRFGVFVMLYCSTT
                     ILLKNELGINKEVADKMFIYYSYGVGIYMAGALLPWVFRQKHNIMNIFEKDKNIKSKE
                     EN"
BASE COUNT          233 a           67 c          122 g          181 t
ORIGIN      
        1 ttttaccagc ccacattaag catggtttca atcagattag agcatcatat caaagatctg
       61 gctacatcaa tcaagctctt gcagttatac cgtttaaggc ttatgtgaac gagccagcag
      121 atagttttaa agcagatata tattggagta tttaacaaaa gagaggtgag tatgggaacg
      181 gaaacaagag agaaagaaat agaaacatgg tcagatttta ttgatgagtt tcttaaagaa
      241 ggtggtgtta tttatgaaaa tcaaaacaaa cagaaaagag aggtagtata tgaaagataa
      301 aataataaga atattaaaag agtattcaga tgatgatagc aaaagaggta aattattaaa
      361 agatttctct atgttcgtta ttaggtttgg ggtttttgtt atgctgtatt gctcgacaac
      421 cattctctta aaaaatgagt tgggcataaa caaagaagtc gctgataaaa tgtttatata
      481 ctattcatat ggagttggta tttatatggc aggagcttta ttaccttggg tgtttcgtca
      541 aaaacacaac attatgaata tttttgaaaa ggataagaat atcaaatcaa aagaagaaaa
      601 tga
//
LOCUS       DAJVQR010000318          591 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVQR010000318 DAJVQR010000000
VERSION     DAJVQR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          217 a          105 c          121 g          148 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaaa t
//
LOCUS       DAJVQR010000319          590 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVQR010000319 DAJVQR010000000
VERSION     DAJVQR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            206..>590
                     /locus_tag="OQM52_004319"
     CDS             206..>590
                     /locus_tag="OQM52_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCS1565891.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIK"
BASE COUNT          159 a          122 c          145 g          164 t
ORIGIN      
        1 tgccaacaat cttgcaaaaa aaatgcgatt gctaaaacaa aagtgcaatt tgcggcaata
       61 tagttattcc atgaccgtac aatcttgcat tcagtgtaaa taatcttgtt tagctctcgc
      121 taaacgcggt tgtataaccg caatacacgc cgtattttat ccggcatata ttgtttttgc
      181 ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt tttgataatg ccagtgaaac
      241 gctgtttgtc gccggtaaaa cgccacggct ttcacgtttt gcatttagcg atgatccaaa
      301 atgggagtct ggacatcacg ttcatgacaa tgaaaccgag ctgatttacg tcaagaaagg
      361 ggttgcaagg tttaccatcg attcttcgtt atatgtcgca catgcggatg acattgtggt
      421 gatagaacgc ggcaggctgc atgcggtggc ctctgacgtt aacgatccgg caacgacgtg
      481 tacctgtgcg ctgtacggct ttcagtttca gggggctgag gaaaatcagt tactgcaacc
      541 gcattcttgt ccggtaattg ccgcggggca gggaaaagaa gtcataaaaa
//
LOCUS       DAJVQR010000320          582 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVQR010000320 DAJVQR010000000
VERSION     DAJVQR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..438
                     /locus_tag="OQM52_004320"
     CDS             <1..438
                     /locus_tag="OQM52_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1565892.1"
                     /translation="TDNKKTETDAIYDKVRSSYLLSCILKKNKNVGLILHAPSFVSVS
                     EKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFVHKIKLTEAIRKL
                     RRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          197 a          101 c          115 g          169 t
ORIGIN      
        1 actgataata aaaagacgga gacagatgcc atttacgata aagttcggag ttcttatttg
       61 ctttcatgta ttctgaaaaa aaacaaaaat gttgggctta ttcttcatgc accatcattt
      121 gtttctgtgt ctgaaaaaat cgccagaatt gtgatggcaa attactcccg taactggagt
      181 aattccgagt tggccagtgc ggttttaatg agtgaatctt ctttaaaaag aagaatgtac
      241 aaagaggtcg gttctataag tacatttgta cataaaataa agcttacaga agctatcagg
      301 aaattacgaa ggacaaatac tcccatttct gtcatatcat ctgagttagg ttacagttca
      361 ccatcgtatt tttctaaagt cttctttaaa tatttaaaga cataccctca aaacataagg
      421 aagaaaaatg gtcggtaatg tcaccaatta ggaggacttg cagagccgct tttttacgat
      481 actgagatca aacagacata atctatgcgg agatttacag cagggcatgg ttcacaaatc
      541 tgacagcgat gaattatcgg cactgcgggc agaaaatgct cg
//
LOCUS       DAJVQR010000321          571 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVQR010000321 DAJVQR010000000
VERSION     DAJVQR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            397..>571
                     /locus_tag="OQM52_004321"
     CDS             397..>571
                     /locus_tag="OQM52_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565893.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADT"
BASE COUNT          214 a           95 c           82 g          180 t
ORIGIN      
        1 acgcaagcac cttaaaatca ccatcataca ctaaatcagt aagttggcag cattaccctc
       61 tttttgttga gcgcccatga ttctttcatt tattgttact aattttttat gttatcatca
      121 tgttatgagc atgccctgtt ttgcgtctct attttatcta tcatattctg agcattaact
      181 acataacaag gcgcaagata tatatccaaa aacaaataat actgttgcaa taattccttt
      241 cagtaaagga agtgtgatta aactcagggg atttcagagt gaaaatcgta caacatatta
      301 aaatatgttc gttataatac atacaagaaa atattaagtt agataatatt attctaaata
      361 actgcagata aaaacgcaca taatggggga tgttttatga aaatacctga agcagtaaat
      421 catattaatg ttcagaacaa tattgatctt gttgatggaa aaataaatcc gaacaaagat
      481 acaaaagcat tacaaaaaaa catatcatgc gtaacaaact catcctcttc tggcataagt
      541 gaaaaacatt tagatcactg tgctgatact g
//
LOCUS       DAJVQR010000322          570 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVQR010000322 DAJVQR010000000
VERSION     DAJVQR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(41..570)
                     /locus_tag="OQM52_004322"
                     /pseudo
     CDS             complement(41..570)
                     /locus_tag="OQM52_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
BASE COUNT          156 a          177 c          127 g          110 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga
//
LOCUS       DAJVQR010000323          552 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVQR010000323 DAJVQR010000000
VERSION     DAJVQR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>552
                     /locus_tag="OQM52_004323"
     CDS             <1..>552
                     /locus_tag="OQM52_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1565894.1"
                     /translation="ETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIE"
BASE COUNT          168 a          123 c          134 g          127 t
ORIGIN      
        1 cagaaacagg ctctgactgg gttaaacccc gctttgatgc ggaaaatatc gaattattta
       61 cggcaaaaac ccgctcgttt gttgatctgc ttgaagccat taatgaagcg gaaagcagcg
      121 gttccgtaat gatcatcgac tccatcagtc acttctggac cggattatgc gacgagtatg
      181 ccagacgccg aaaccgtaaa cgaggactgg aattttcaga ctgggcgtgg ctcaaacagg
      241 agtggcgacg ctttaccgat cgatttgtta acagtcaggc gcacattatt atgtgcgggc
      301 gtgcaggata tgagtatgac tttttcgaag gtgatgatgg caaacgccag ttggcaaaaa
      361 ccggtataaa aatgaaagca gaaacagaaa ccggttatga accctccatc cggatccaga
      421 tggagaagca aatgaacata gaaaccggtc aggtgtggcg caccgccaga atactaaaag
      481 atcgctccac ccgtattgat ggacaggtat tttcaaaccc aacatttaaa aactttcttc
      541 ctcatatcga gt
//
LOCUS       DAJVQR010000324          548 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVQR010000324 DAJVQR010000000
VERSION     DAJVQR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            20..337
                     /locus_tag="OQM52_004324"
     CDS             20..337
                     /locus_tag="OQM52_004324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565895.1"
                     /translation="MLILALVFVSFIESTENKISIEENSLYNLQTDVMNIMLYDTPIP
                     IIKSLTEEQKSKLIIINKDIDNCMERKQQLGASGLSECYKKNEFLFNELKVGFSDRLI
                     FNN"
BASE COUNT          221 a           76 c           78 g          173 t
ORIGIN      
        1 attaattgaa gtgtttatta tgttaatact ggcattagtt tttgtatctt ttattgaaag
       61 caccgaaaac aaaataagca tagaagaaaa tagtttatat aacctacaaa ctgatgttat
      121 gaatatcatg ctttatgaca caccaatacc aataataaaa tcattaacag aagagcaaaa
      181 atcaaagcta attataataa acaaggatat agataattgt atggaaagaa agcagcaatt
      241 aggtgcttca ggattatcag aatgctataa aaaaaatgaa ttcttattta atgagcttaa
      301 agttggcttt tcagatagat tgatttttaa taattaattt atcagacagg cgggatagtt
      361 agacacaaaa aaacatggag cctaaacccc atgttctaat cgttgttata tattcggacg
      421 caatctacaa tatataactt atcaaattaa acatcctata agatgagaaa tacccataac
      481 ccatacttac ttatatttca taacgcttta attaattgtt gtcaagttta agaacagcat
      541 ttccggga
//
LOCUS       DAJVQR010000325          545 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVQR010000325 DAJVQR010000000
VERSION     DAJVQR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(118..>545)
                     /locus_tag="OQM52_004325"
     CDS             complement(118..>545)
                     /locus_tag="OQM52_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1565896.1"
                     /translation="SWRRAVLSLEEHYKAWLLWCYSGSICWEYQITITHWAWEEFKAH
                     SGTRKIAEKTQERLKKLIWLAAQAVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGH
                     WVVMKHIFHRLDSEALLFVMRTRSEQKAAFSKQSIAKVD"
BASE COUNT          138 a          143 c           98 g          166 t
ORIGIN      
        1 tttaacgtcc tgtgccattt ttcaggcata aaaaaaccgc tcggcggcgg gttttgctta
       61 ctttaccatc gcgtacaaaa tcggcaaagt atcagatttg catgagatat atgcctttta
      121 atctactttt gcaatacttt gctttgaaaa tgccgctttt tgttctgaac gtgttctcat
      181 cacaaacaat aaagcctcac tatccagtcg gtgaaaaatg tgtttcatta caacccagtg
      241 accagtaaat gttttggacc agtttttggt tgttactccc gccagtaatg ccagctcctg
      301 atattcataa ccttccccac caaaaagttc tgcttttacc gcctgcgccg ccagccagat
      361 taattttttc aggcgttcct gtgttttctc tgcaattttc ctagtgccag aatgagcctt
      421 aaactcttcc cacgcccagt gggttatggt gatctgatac tcccagcaaa tacttccgct
      481 gtaacaccac aacaaccagg ctttatagtg ctcttcaaga gacagaacag cccgccgcca
      541 cgatg
//
LOCUS       DAJVQR010000326          543 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVQR010000326 DAJVQR010000000
VERSION     DAJVQR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(269..>543)
                     /locus_tag="OQM52_004326"
     CDS             complement(269..>543)
                     /locus_tag="OQM52_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCS1565897.1"
                     /translation="SAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSV
                     NPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          134 a          168 c          145 g           96 t
ORIGIN      
        1 agcgcgcagg gggcagccag tcgccgccgc cccctgcacc cccgggctct ggcgaacaaa
       61 atcgccgctg cgcggtacct tcagcttatc cccttcggca accgggtcgg gcgcgaggta
      121 acgtcctgta agacgcgccc tcagcccaca tccatgtggg ctgccccggc cttcggggaa
      181 cgcttcagcg attttgacgc caccaatacc cgtgcggttt gtaatgtcga agataaaaca
      241 accagggcag ataaaatgcc aatgctaatt aatcacgctt ttccagcaga gtccggtaaa
      301 tcagaccacc gataatgccg ccgacaattg gcaccaccca gaagaaccac agttgttcta
      361 atgcccagcc gccctggaag atagcaaccg cggtgctgcg cgccgggtta acagaagtgt
      421 tagtcaccgg aatactgatt aagtgaatca gggttaaggc cagaccaata gcgatcggcg
      481 caaaacctgc cggagcgaat ttgtcggttg caccgtggat cactaacagg aagcccgcac
      541 tca
//
LOCUS       DAJVQR010000327          542 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVQR010000327 DAJVQR010000000
VERSION     DAJVQR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>542
                     /locus_tag="OQM52_004327"
     CDS             <1..>542
                     /locus_tag="OQM52_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1565898.1"
                     /translation="KLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPL
                     YRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLA
                     PGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAY
                     AGYNVLYETGRVKEAGCLAH"
BASE COUNT          133 a          136 c          161 g          112 t
ORIGIN      
        1 ccaaactggc ctgtagccgg tgtgatgtca tcgttcaggc accacttcct cctaaaccga
       61 tcgaacgcgg ttatgccagt gcagggttac ttgcacggat cctggtcagc aaatatatgg
      121 aacatatccc tttatatcgc cagtcagaaa tatacgcgcg acagggcgtg gagctgagcc
      181 gtaataccat ggtgcgctgg gtatcagaaa tggcagacaa actccgtcct ctgtatatag
      241 cgctgaatga ctatgttctg gaggcaggaa aggtgcacgc agatgacact ccggtgaaag
      301 tactggcccc ggggaacgga aagacgaaaa cgggtcgtct gtgggtatac gtcagggatg
      361 atcgtaatgc gggttcatcc ctgccggcag ccgtctggtt cgcgtattcg gcagatcgca
      421 aaggagaaca tccgcagctc cacctggcaa agtatcaggg cgtactgcag gctgatgcct
      481 atgcaggtta taacgtactg tacgaaacgg gccgggtgaa ggaagccggg tgcctggccc
      541 ac
//
LOCUS       DAJVQR010000328          538 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVQR010000328 DAJVQR010000000
VERSION     DAJVQR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            25..>538
                     /locus_tag="OQM52_004328"
     CDS             25..>538
                     /locus_tag="OQM52_004328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565899.1"
                     /translation="MSKDNDLNDFEQAFNMEAAMPDFPDYEIVADNVAPAAAPVKKPE
                     PEPEVNLEHDMMEVKSRIDSFKDVLNIGDKFGMSDEQRKMIKNQLSELELTYVKLQKT
                     LNLEEPAPTASLIEKKPEEAKPTVPAAVVSAADSIPAQDFTDLDKFTQMKNEEAKQTF
                     KAKSLKDRIKS"
BASE COUNT          181 a          112 c          123 g          122 t
ORIGIN      
        1 acataaaaaa cgcgaggtaa aaacatgagt aaagataacg atttaaatga ttttgaacag
       61 gctttcaata tggaagcggc aatgcctgat ttccctgatt atgagattgt ggccgacaac
      121 gtagccccag ctgcagctcc ggttaagaag ccagaacctg aaccagaagt taaccttgag
      181 catgacatga tggaagtcaa aagtcgtata gatagcttca aggatgtgct caacatcggt
      241 gacaagttcg gtatgagtga tgagcaaagg aagatgatta agaaccagct ttcagaactg
      301 gaactcactt acgttaagct acagaagaca cttaaccttg aagagccagc tcctaccgct
      361 tccctgatag agaagaaacc agaagaggcc aaaccaaccg ttccggctgc tgttgtctct
      421 gctgctgata gcataccagc tcaagacttc actgatctgg acaagtttac tcagatgaag
      481 aatgaagagg ctaaacagac ttttaaggct aagagtctta aagaccgtat caagtctg
//
LOCUS       DAJVQR010000329          535 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVQR010000329 DAJVQR010000000
VERSION     DAJVQR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          174 a           78 c          107 g          176 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc
      361 tcttattttc cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga
      421 tctcatttcg agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg
      481 attggggttt tatgagaaat aaagagacgt attgtctttg tttaaacggt acgta
//
LOCUS       DAJVQR010000330          533 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVQR010000330 DAJVQR010000000
VERSION     DAJVQR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..274
                     /locus_tag="OQM52_004329"
     CDS             <1..274
                     /locus_tag="OQM52_004329"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1565900.1"
                     /translation="KRIILASSNPGDIVLDPFAGSFTTGAVAIASGRKFIGIEINSEY
                     IKMGLRRLDVASHYSAEELAKVKKRKTGNLSKRSRLSEVDPDLIAK"
     gene            358..>533
                     /locus_tag="OQM52_004330"
     CDS             358..>533
                     /locus_tag="OQM52_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1565901.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
BASE COUNT          150 a          118 c          123 g          142 t
ORIGIN      
        1 gaaacgcatt attctcgcct cttccaaccc aggcgatatc gttctcgacc cgtttgccgg
       61 tagctttact accggtgccg tagccatcgc cagcggacga aaattcattg gtattgagat
      121 caacagcgag tacatcaaaa tggggcttcg acggctggat gtcgcgtcgc attactccgc
      181 ggaagaactg gcgaaagtga aaaaaagaaa gaccggcaac ctgtcaaaac gaagccggtt
      241 aagcgaagtt gaccccgatc tcattgcaaa gtaaaggaag tgtaagccta gttttcagat
      301 tattcatttc gtgtatattt cctgccagac ttggttaaac atgcacaggc tcaggtaatg
      361 attcgcaagt attggtggct cgtcgttttc gctgtcttcg tttttctgtt tgatacttta
      421 ctgatgcagt ggattgaact gctgacaaca gaaacagaca aatgccgtaa tatgaactca
      481 gttaatccac taaaactggt taactgtgac gaactgaatt ttcaggacag aat
//
LOCUS       DAJVQR010000331          524 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVQR010000331 DAJVQR010000000
VERSION     DAJVQR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(35..307)
                     /locus_tag="OQM52_004331"
     CDS             complement(35..307)
                     /locus_tag="OQM52_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565902.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          117 a          132 c          147 g          128 t
ORIGIN      
        1 tgtgaacagg cgtttcagcg gtcggggctg aaaatcacca ccacttcggg tcatccgcct
       61 tcatctccgc ttctgtttcg tataaatcaa aacgacggca ggtatggcag aacgtgacgt
      121 attcgtacgg tgaatccggg tacattttgt ggaaggtttc ccagaaacag accgtgcagg
      181 agggatgacc ggtgataaag tctgcaaata cggacttata agggtgatta ttggctctgg
      241 cgacggcttt cagaacctct ttcaccattc tggtgtggac tttctggtgc tccaggttgt
      301 gtgacatggg aactcattct ggatggttac tctgaaagct catattctgc cacaccccga
      361 tttgcagccg ccaggctgcc gtggttcaca gataaaacac tctccaggaa acccggggcg
      421 gttcactgcg tgtctgggtt cgccagcatg agcgggatac cgggggcggt tcacacgaac
      481 ataccacaga cagttgattt tggtacgccc aggcgacgtc cggc
//
LOCUS       DAJVQR010000332          511 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVQR010000332 DAJVQR010000000
VERSION     DAJVQR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..511
                     /locus_tag="OQM52_004332"
                     /pseudo
     CDS             1..511
                     /locus_tag="OQM52_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAJVQR010000333          510 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVQR010000333 DAJVQR010000000
VERSION     DAJVQR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          160 a          102 c          124 g          124 t
ORIGIN      
        1 acaccattca gccggggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac
       61 ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aacccgctgt
      121 cagcacgttc aggaaggggg tgatgtacaa atggcacaga ggatgaaagt gacgtatgag
      181 ctgaaggaat gatgtaaaag tgatgtataa gtgatgtaaa agtgatgtat gaactgaagg
      241 agcgatgtaa ttctgtagtc aggaagaata acccgttcat gcaggcatga acgatacagt
      301 gaggaacgct ttttttgtca cgatctcccc ttaacctgcg taaatcaccc gccatggttg
      361 attatttttc aacctcaaaa tggtcataca cctaacgcag ccgcgatttt catcgcaact
      421 gcatgaatta aaagaaaaaa aaggaatatt tcgttttcac gagtaatacc cgggtattaa
      481 aaatccactg taattacatg aaaataaaga
//
LOCUS       DAJVQR010000334          507 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVQR010000334 DAJVQR010000000
VERSION     DAJVQR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            43..288
                     /locus_tag="OQM52_004333"
     CDS             43..288
                     /locus_tag="OQM52_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009351656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HCS1565903.1"
                     /translation="MEKVNIYDAKTNLSKIVQEVARTGEPVVIAKNGHALVKVIAYRE
                     EKPKRKLGFLKGKGSVPANFDDMNSNEIVDMFEGKYS"
     gene            288..>507
                     /locus_tag="OQM52_004334"
     CDS             288..>507
                     /locus_tag="OQM52_004334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HCS1565904.1"
                     /translation="MDLLLDTNILLFMAYEPDKLKQEVVELLEDTEKTLCFSAASVWE
                     VVIKSGLNKPDFDVDPEQFRDGLFEAGLI"
BASE COUNT          152 a           93 c          132 g          130 t
ORIGIN      
        1 acagggagta agcttaaaat gtgttcaatg cgaggtaata ctatggaaaa ggttaacatc
       61 tatgatgcca aaactaacct gtccaagatc gttcaagagg tcgcccggac tggtgagcca
      121 gttgtgatcg caaagaacgg tcacgctctg gtaaaagtca tcgcctacag agaagagaag
      181 cctaaacgca agctgggttt ccttaagggt aagggtagcg ttcctgccaa ctttgacgac
      241 atgaacagca atgagatcgt ggatatgttt gaaggaaaat actcataatg gatttgctgt
      301 tagataccaa cattctcttg ttcatggcct atgaaccgga caagctgaaa caagaggttg
      361 tagagctgct ggaagacact gaaaagaccc tttgctttag tgctgccagt gtttgggaag
      421 tcgtcattaa aagcggtttg aacaagcctg atttcgatgt agatccagaa cagtttagag
      481 atggcctgtt tgaagctggt ttgattg
//
LOCUS       DAJVQR010000335          505 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVQR010000335 DAJVQR010000000
VERSION     DAJVQR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..505
                     /locus_tag="OQM52_004335"
                     /pseudo
     CDS             1..505
                     /locus_tag="OQM52_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          113 a          124 c          155 g          113 t
ORIGIN      
        1 tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag cgcacgcgcc
       61 cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt gtcgcgtgcg
      121 cagttgcacg tcattctcag acgaaccgat gactggatgg atggccgccg cagtcgtcac
      181 actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct gcccacgtat
      241 ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg tatgcctgcg
      301 atcaatgcca aacgtgttta ccggctcatg cgccagaatg cgctgttgct tgagcgaaaa
      361 cctgctgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa agaaagcaat
      421 cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag actgcgtgtc
      481 acgttcgcgc tggactgctg tgatc
//
LOCUS       DAJVQR010000336          502 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVQR010000336 DAJVQR010000000
VERSION     DAJVQR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            36..209
                     /locus_tag="OQM52_004336"
     CDS             36..209
                     /locus_tag="OQM52_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565905.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(281..430)
                     /gene="hokB"
                     /locus_tag="OQM52_004337"
     CDS             complement(281..430)
                     /gene="hokB"
                     /locus_tag="OQM52_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1565906.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT          128 a          113 c          135 g          126 t
ORIGIN      
        1 atcgcacata agattcgata aaccatggag gttatatgaa aaaactggca cttattttgt
       61 ttatgggaac gcttgtttcc ttttatgccg atgccgggcg caaaccctgt tctggttcga
      121 aaggggggat ctcacactgt acggcaggcg gcaaatttgt ctgtaatgat ggttctatta
      181 gtgcatcgaa aaaaacatgc actaactgaa gtgtaaaagg ggtgccatga gaaaatgaat
      241 ctgctagtca gatgcgcggg gaaaatcccc gcgcttgccc ttacctggac gtgcaggcca
      301 tgagcgcagc aacctcctta tcaccgtccc ggaaccgcag ttcgtagagc gtttgtcggg
      361 tcaggagtgt gaatgccaga atcgtaatgc agataatgag cagacacacc accagagggt
      421 tgcgcttcat agcctttctc cttgccggat ggcgggtaag aggctaacaa tctgaattgc
      481 ttggttcatt cgttggcctc gg
//
LOCUS       DAJVQR010000337          497 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVQR010000337 DAJVQR010000000
VERSION     DAJVQR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>497
                     /locus_tag="OQM52_004338"
     CDS             <1..>497
                     /locus_tag="OQM52_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1565907.1"
                     /translation="VPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVP
                     QPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVA
                     WINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMR
                     YDANT"
BASE COUNT          164 a          112 c          123 g           98 t
ORIGIN      
        1 ctgttccaga atacacgcca aaaccagcgt ccgttgcaac gaaggctacg catctttcat
       61 ttataaaggc cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa
      121 ttattaaagt gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac
      181 aaaggctgat tgatgaatgt ccggagccat taaagtctgt tgttgaattt gcactggcaa
      241 caggcttaag acgctcgaac atcatcaacc ttgaatggca acaaatagat atgcagcgcc
      301 gggtggcatg gataaacccg gaagagagta aatcaaaccg cgcaattggc gttgcgctga
      361 atgatactgc atgtcgcgta ttgaaaaaac aaatcgggaa tcatcaccgt tgggtatttg
      421 tgtacaagga aagctgtacc aaaccagacg gaacgaaagc gccaacggtc aggaagatgc
      481 ggtatgacgc aaacaca
//
LOCUS       DAJVQR010000338          481 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVQR010000338 DAJVQR010000000
VERSION     DAJVQR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>481)
                     /locus_tag="OQM52_004339"
     CDS             complement(<1..>481)
                     /locus_tag="OQM52_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HCS1565908.1"
                     /translation="DKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEK
                     PVTHVSRDELQALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNP
                     GNSSLGLMTAFFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGE
                     "
BASE COUNT           93 a          122 c          138 g          128 t
ORIGIN      
        1 cttcgccgcg atcggtgagg ttcccgccgc cagcattagc gccggaagcc gtgcgcgtga
       61 tgcgtgaaac acgatttcct ttcatccact cttttgtcag cagaccgcga tcggtgtagt
      121 ctgcgtccat gtatgcttcg aaaaaagcag tcatcagccc caggcttgaa ttgcctggat
      181 tagggaaaac tctgtcagta tcacgcacca gtttgtggag atcgcgaatt tccagcggat
      241 cgagcaggct ggttttgtgg gaaacagcca gggcagtaac agctggtagt tcttcatccc
      301 gagcaatgtg taatgcctgg agttcgtcgc gtgaaacgtg cgttactggt ttttcgctgc
      361 cgtgttgcgc aagccagcga atgggcagtt cctgaccgga aactggcagg agcatgttct
      421 cctcaatttc tgtcatgtct tcgccgttga cgttggtatt gtcagtgctg gccggtttgt
      481 c
//
LOCUS       DAJVQR010000339          481 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVQR010000339 DAJVQR010000000
VERSION     DAJVQR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(151..>481)
                     /locus_tag="OQM52_004340"
     CDS             complement(151..>481)
                     /locus_tag="OQM52_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1565909.1"
                     /translation="SPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          123 a          123 c          105 g          130 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 a
//
LOCUS       DAJVQR010000340          476 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVQR010000340 DAJVQR010000000
VERSION     DAJVQR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            187..>476
                     /locus_tag="OQM52_004341"
     CDS             187..>476
                     /locus_tag="OQM52_004341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565910.1"
                     /translation="MIKFLIGFLIEIKILTLKMKIDKASHVLSRAMLSKGYSKDFNDK
                     CKTVRKKLLALYDKDLKLPDNYRNAEYMLGEAMVLRNKFNYLIEKSLITEHK"
BASE COUNT          196 a           56 c           77 g          147 t
ORIGIN      
        1 cggaaacaaa aaactcatta aaaaaatagt ggactgtgcc gatgattatc ataatgactg
       61 ctatgtaatc tactcatttg aaaagtatta tagcatcgat gatttttaca acattaggtt
      121 ttaacgatta cataaacagc taacatctaa cacagtcccg aaaggggcta tgataaggag
      181 aataatatga ttaagttttt aataggtttt ttaatagaaa taaagatatt aactttaaag
      241 atgaaaattg ataaagcctc acatgtatta agtagggcta tgctttcaaa agggtatagc
      301 aaagacttta atgacaaatg taaaacagta agaaaaaagc ttttagcttt atatgataaa
      361 gatttaaaac tacctgataa ttatagaaat gctgaatata tgttaggtga agcaatggtt
      421 ttgcgtaata agtttaacta ccttattgaa aaatcactta ttacagaaca taaaag
//
LOCUS       DAJVQR010000341          456 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVQR010000341 DAJVQR010000000
VERSION     DAJVQR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..346
                     /locus_tag="OQM52_004342"
     CDS             <1..346
                     /locus_tag="OQM52_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="with lsrACD is involved in the transport of
                     autoinducer 2 into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1565911.1"
                     /translation="AAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQ
                     QGKISVYVADVLLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERV
                     IFNKENIGKYDF"
BASE COUNT          143 a           86 c          121 g          106 t
ORIGIN      
        1 cgctgccgca caagccgcag aaaacctgaa aaatgacaaa gtagcgattg tcggattcag
       61 tacgccaaat gtgatgcgcc cgtatgtaga gcgcggcacg gtgaaagaat ttggactgtg
      121 ggatgtggtt cagcaaggca aaatttcagt gtatgtcgcg gatgtattat tgaaaaaagg
      181 atcaatgaaa acgggcgaca agctggatat ccagggcgta ggtcaggttg aagtctcgcc
      241 aaacagcgtt cagggctatg actacgaagc ggatggtaat ggcatcgtac tgttaccgga
      301 gcgcgtgata ttcaacaaag agaatatcgg caaatacgat ttctgatgtg cattacttaa
      361 ccggagtaag ttatggcaga tttagacgat attaaagatg gtaaagattt tcgtaccgat
      421 caaccgcaac aaaatatccc ttttaccctg aaaggt
//
LOCUS       DAJVQR010000342          455 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVQR010000342 DAJVQR010000000
VERSION     DAJVQR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>455)
                     /locus_tag="OQM52_004343"
     CDS             complement(<1..>455)
                     /locus_tag="OQM52_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1565912.1"
                     /translation="GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVA
                     AYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA
                     FTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          108 a          125 c          124 g           98 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg cccctgtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacc
//
LOCUS       DAJVQR010000343          450 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVQR010000343 DAJVQR010000000
VERSION     DAJVQR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            101..>450
                     /locus_tag="OQM52_004344"
     CDS             101..>450
                     /locus_tag="OQM52_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1565913.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          111 a          125 c          116 g           98 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat
//
LOCUS       DAJVQR010000344          446 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVQR010000344 DAJVQR010000000
VERSION     DAJVQR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>446)
                     /gene="ospC2"
                     /locus_tag="OQM52_004345"
     CDS             complement(<1..>446)
                     /gene="ospC2"
                     /locus_tag="OQM52_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1565914.1"
                     /translation="KSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSEIIQKTHPLSSD
                     ERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFDKIKSENIQTYKNTFSEDIEEIANH
                     DFVFFGVEISNHQETLPLNKTHHTVDFGANAYIIDHDSPYGYMTLT"
BASE COUNT          146 a           61 c           88 g          151 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgattt
//
LOCUS       DAJVQR010000345          439 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVQR010000345 DAJVQR010000000
VERSION     DAJVQR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..269
                     /locus_tag="OQM52_004346"
     CDS             <1..269
                     /locus_tag="OQM52_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1565915.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>439
                     /gene="tnpB"
                     /locus_tag="OQM52_004347"
     CDS             266..>439
                     /gene="tnpB"
                     /locus_tag="OQM52_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1565916.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILW"
BASE COUNT          109 a          114 c          125 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtgg
//
LOCUS       DAJVQR010000346          434 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVQR010000346 DAJVQR010000000
VERSION     DAJVQR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            35..434
                     /locus_tag="OQM52_004348"
                     /pseudo
     CDS             35..434
                     /locus_tag="OQM52_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJVQR010000347          433 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVQR010000347 DAJVQR010000000
VERSION     DAJVQR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>433
                     /locus_tag="OQM52_004349"
     CDS             <1..>433
                     /locus_tag="OQM52_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1565917.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTV"
BASE COUNT          114 a           96 c          103 g          120 t
ORIGIN      
        1 tgatggcgag attttaaaat ccgcttgtga aatcaatgac tctgataaga aaattgaagt
       61 tgctcttggt cactataatg ccgaacagtt tcgttctgtt ggcgatcgta gcccaaaaat
      121 tccattcact attcctttag ttaactgccc agtgactggg tgggagcacg ataacggcaa
      181 cgttgaagcg tctttccgtt tatggctgga aacgcgggat aatggcaccg tccctaactt
      241 ccctaatctg gcaaaagtgg gctcttttgc tggcacagcg acaacaggtg tgggtatccg
      301 tattgacgat gcggaaagcg gaaaccttat gccactgaat gctatgggga atgataacac
      361 gatttatcag atcccagcgg actccaatgg tattgtcaat gttgacctca tcgcttacta
      421 cgtatcaacc gtt
//
LOCUS       DAJVQR010000348          405 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVQR010000348 DAJVQR010000000
VERSION     DAJVQR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            18..405
                     /locus_tag="OQM52_004350"
                     /pseudo
     CDS             18..405
                     /locus_tag="OQM52_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           88 a          115 c          109 g           93 t
ORIGIN      
        1 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
       61 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact
      121 gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga
      181 aaatcattga tatggccatg aatggcgttg gatgccgggc aaccgcccgc attatgggcg
      241 ttggcctcaa cacgattttc cgccatttaa aaaactcagg ccgcagtcgg taacctcgcg
      301 catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt ggggatacgt
      361 cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacag
//
LOCUS       DAJVQR010000349          405 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVQR010000349 DAJVQR010000000
VERSION     DAJVQR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          126 a           66 c           92 g          121 t
ORIGIN      
        1 ttattgtagt gatcataggc atgattatct atctacctat catccttgtg ctgatgacag
       61 gattgatgat aagagagttg atagacacgg ttaggggatg gtgatgcaag gggctgtaag
      121 cccctttgtc ttgcccgttc tgtagctcta cagggtagaa accagcccca acacatggta
      181 tgttggttga gttaagtcct tatgtgtgta tctcatgctc aaatacaaaa gcttgaaaga
      241 ttttcttgat gaacagaaac agcaagagct ttataaaaaa aggcttgctg aaaagctcta
      301 ttacactgtt aaaaagggaa catcagaaga gatattatca gttttcaagc agtgttctga
      361 aagtggttta gatttcaaac aggtgaaaca tgactactta cttga
//
LOCUS       DAJVQR010000350          398 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVQR010000350 DAJVQR010000000
VERSION     DAJVQR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>398
                     /locus_tag="OQM52_004351"
     CDS             <1..>398
                     /locus_tag="OQM52_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1565918.1"
                     /translation="DFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFS
                     FAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVN
                     KTQNFEAVLQYQFDFGLRPSIGYSYSKALD"
BASE COUNT          111 a           78 c           94 g          115 t
ORIGIN      
        1 agatttcttt ggcatggttg aaggtctaaa ttttgctctg caatatcagg gtaaaaatga
       61 aggtacaggc aactacaaag caaatggcga tggccatggt ttatctgcaa cttacactat
      121 tgacggtttc tcatttgctg gtgcttatgc aaactctgac cgtactgatt ggcagtctgg
      181 tgatggtaaa ggcgaaagag ccgaagtttg ggcgttgtca acaaaatatg acgctaacaa
      241 tgtttatgct gctgtgatgt acggtgaatc tcacaacatg aattccgacg acggtgatgt
      301 tgtgaacaaa acacaaaact tcgaagctgt tctgcaatat cagttcgact ttggtctgcg
      361 tccatccatc ggttactcct attctaaagc actggacg
//
LOCUS       DAJVQR010000351          398 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVQR010000351 DAJVQR010000000
VERSION     DAJVQR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(9..>398)
                     /locus_tag="OQM52_004352"
     CDS             complement(9..>398)
                     /locus_tag="OQM52_004352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565919.1"
                     /translation="LKAEALINAGININAKDKDGNTALFFSDYMTSNLLLNKGANVNH
                     RNNIGMTAIFRADRSKASLLIKAGARVNFKSYVDNTTALQMAYIRRDEAVMTVLIEAG
                     ADIHHNWRGYSAQAKAFMTTINERQAA"
BASE COUNT           94 a           82 c           76 g          146 t
ORIGIN      
        1 tttccccctt atgctgcttg tctttcgtta atagttgtca tgaatgcttt agcttgagct
       61 gagtagcctc tccaattgtg gtgtatgtct gcccctgctt cgattagaac ggtcataaca
      121 gcctcatcac gtcttatgta agccatttga agagctgtag tgttgtctac gtaggatttg
      181 aagttaactc ttgctcccgc ctttataagc aagcttgctt ttgatctgtc tgctctgaaa
      241 atcgctgtca taccaatgtt gtttctatga ttgacattag cccctttgtt taaaagaagg
      301 ttagaagtca tataatcaga gaagaacaac gctgtattgc cgtctttatc tttagcattt
      361 atattgatac ccgcatttat taaagcttct gctttaag
//
LOCUS       DAJVQR010000352          394 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVQR010000352 DAJVQR010000000
VERSION     DAJVQR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(137..>394)
                     /locus_tag="OQM52_004353"
     CDS             complement(137..>394)
                     /locus_tag="OQM52_004353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565920.1"
                     /translation="KVMGGSEEALSNAKSNTTNTVERAGQATASSMSNMARNREKKRD
                     IEPKGGNEGNIGKGSNQEPTNNTPTDKGNHGQSIKDLNPKE"
BASE COUNT          102 a           80 c           54 g          158 t
ORIGIN      
        1 tctttatctt tcataaatat ctccttcttt atatgtatat aaattaatta acacataaaa
       61 taaacacgtc aatagaattg ataaaaaaaa ggtagcaacc cgctaccttt tagttagttt
      121 cagataaaag gattacttat tccttcgggt ttaagtcttt tatagactga ccatgatttc
      181 ctttatctgt tggcgtgtta ttggttggct cttgattact acctttacca atattacctt
      241 cattgccgcc tttaggttca atatctcttt tcttctcacg gttccttgcc atatttgaca
      301 tagaacttgc tgtagcttgt cctgctcttt cgacagtatt tgtagtattg cttttcgcat
      361 tactaagagc ttcttctgaa ccgcccatta cttt
//
LOCUS       DAJVQR010000353          392 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVQR010000353 DAJVQR010000000
VERSION     DAJVQR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          129 a           87 c           56 g          120 t
ORIGIN      
        1 ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact tcatgcccat
       61 ttatgcccga tgtttccccc ccaccggggg atcaatcatc aataaatatg gcgttctttt
      121 ttaattggct agcaacatac cacccctcat ttgtatgaca aatgataatc tttcatccaa
      181 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atggggaaat
      241 cgacgatcat ttttggcata agaaatccat ttaaaaatgg ctacatccct tatacctatt
      301 cagagctact tattcagggg ttattgagat atacactgat ggtgtacaaa aaaatcaccg
      361 acaataatca cgtatcaagt aatagtcgga at
//
LOCUS       DAJVQR010000354          392 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVQR010000354 DAJVQR010000000
VERSION     DAJVQR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>392)
                     /locus_tag="OQM52_004354"
     CDS             complement(<1..>392)
                     /locus_tag="OQM52_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1565921.1"
                     /translation="LLELLLTLGVIAGIIIAAFIVFPKVQASQKIDKESRNITAIQAG
                     VKSLYGGRAKISSLNTDSFIASKNAPDNMILDGKLVNEWKGNVSVEYSGNHGKYNIMY
                     DAVPPDACAKLITAVSGNFVSITVYNDNG"
BASE COUNT          108 a           88 c           70 g          126 t
ORIGIN      
        1 ccgttgtcgt tgtatactgt gatactcaca aaattacctg aaaccgctgt aatgagtttt
       61 gcgcaagcat cagggggaac ggcatcatac ataatgttgt atttgccatg attaccgctg
      121 tattcaacgc ttacattgcc tttccactca ttcactaact taccgtccaa gatcatgtta
      181 tccggggcgt tcttagacgc aatgaagcta tcagtattca atgatgagat cttagccctg
      241 ccgccataga gagatttcac tcccgcttga atagctgtta tgtttctgct ctctttatct
      301 atcttttgtg aagcctgaac ttttggaaaa actataaaag cggctattat tattccagct
      361 ataacaccta acgttaaaag caattctaat ag
//
LOCUS       DAJVQR010000355          390 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVQR010000355 DAJVQR010000000
VERSION     DAJVQR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..255
                     /locus_tag="OQM52_004355"
     CDS             <1..255
                     /locus_tag="OQM52_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCS1565922.1"
                     /translation="ADQQFLAEKLGVSRSTIIRWLNYLESKNALVRIPVAGKVCAYAL
                     DPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          107 a           95 c           88 g          100 t
ORIGIN      
        1 gcggatcagc aattcctagc tgaaaaactt ggagttagca gaagcacaat tattcggtgg
       61 ctcaattact tagaatcaaa aaatgcatta gttagaatcc ccgttgctgg taaggtttgt
      121 gcgtatgccc tcgatccaca tgaagtctgg aagggataca acactacgaa aaaccatgca
      181 gcgtttgtca ctaaaacact ggtcaacaaa gacggtgata ttcagcgccg aatcatggcc
      241 atgttttcaa attgagccag cggcaggcgg acaatcaggg gctacgtgtt aacgttttga
      301 ccatgattgt ctatcctgca ttgctctttt gccgcctcaa aatcccttgc gtgtttttgc
      361 tccccgatcc caagaacgac cgcgtcgcca
//
LOCUS       DAJVQR010000356          389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVQR010000356 DAJVQR010000000
VERSION     DAJVQR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>389
                     /locus_tag="OQM52_004356"
     CDS             <1..>389
                     /locus_tag="OQM52_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1565923.1"
                     /translation="ARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTY
                     TGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSIS
                     ASGHKFGLAPLGCGWVIWRDEEALPQEL"
BASE COUNT           75 a          116 c          115 g           83 t
ORIGIN      
        1 gcccgctact gggatgtgga gctgcgtgag atccctatgc gccccggtca gttgtttatg
       61 gacccgaaac gcatgattga agcctgtgac gaaaacacca tcggcgtggt gccgactttc
      121 ggcgtgacct acaccggtaa ctatgagttc ccacaaccgc tgcacgatgc gctggataaa
      181 ttccaggccg acaccggtat cgacatcgac atgcacatcg acgctgccag cggtggcttc
      241 ctggcaccgt tcgtcgcccc ggatatcgtc tgggacttcc gcctgccgcg tgtgaaatcg
      301 atcagtgctt caggccataa attcggtctg gctccgctgg gctgcggctg ggttatctgg
      361 cgtgacgaag aagcgctgcc gcaggaact
//
LOCUS       DAJVQR010000357          384 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVQR010000357 DAJVQR010000000
VERSION     DAJVQR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..353)
                     /locus_tag="OQM52_004357"
     CDS             complement(<1..353)
                     /locus_tag="OQM52_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCS1565924.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFAS"
BASE COUNT          130 a          119 c           73 g           62 t
ORIGIN      
        1 gaagcaaaac cgctggctgc cgcgtcaaaa cccgttttac cactggcaat taaatacagc
       61 agcgccgctg caacaatacc gccgacaacc tgggcaatta cgtagccaac gacttctttt
      121 gccggaaaac gtccgccagc ccataaacca atagtgaccg ccgggttaaa atgaccacca
      181 gaaatatgac caacagcgaa ggccatcgtc agaacggtca aaccaaatgc cagcgccacg
      241 ccggcaaaac caatgcctaa ttccgggaag cctgcggcca gtacagcact accacagcca
      301 ccaaaaacaa gccagaaagt accaaaacat tcagctgcta attttctgaa catatccacc
      361 tcaattaaaa attgaccctg tgaa
//
LOCUS       DAJVQR010000358          381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVQR010000358 DAJVQR010000000
VERSION     DAJVQR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(273..>381)
                     /locus_tag="OQM52_004358"
     CDS             complement(273..>381)
                     /locus_tag="OQM52_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCS1565925.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt t
//
LOCUS       DAJVQR010000359          378 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVQR010000359 DAJVQR010000000
VERSION     DAJVQR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..348
                     /locus_tag="OQM52_004359"
     CDS             <1..348
                     /locus_tag="OQM52_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1565926.1"
                     /translation="IAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALE
                     GCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETS
                     NIQLADSVMTPTY"
BASE COUNT          102 a           89 c           86 g          101 t
ORIGIN      
        1 attgctccag cttgtaccct ggtagctgcg acgaaagatt cagtagtcac attgccaaat
       61 gtcagcgcca ccaagttgca aactaatgga gcagtctctg gcgttaaaac tgatgtacca
      121 attgccttag aaggctgtga tgttactgta actaaaaatg cgacgtttac ctttagtggt
      181 acggctgacg gcgtgcaacc tacagcattt gccaaccagg ctaccaccga tgcagcgacg
      241 aatgttgctc tacaaatgta tctgccagat ggttctacat cggtaactcc cggtaccgaa
      301 acgtcgaata ttcagttagc ggattcggta atgactccaa cttactgata gtgttttatg
      361 ttcagataat gcccgatg
//
LOCUS       DAJVQR010000360          372 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVQR010000360 DAJVQR010000000
VERSION     DAJVQR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>372)
                     /locus_tag="OQM52_004360"
     CDS             complement(<1..>372)
                     /locus_tag="OQM52_004360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565927.1"
                     /translation="NIQQSETEWDYCQIDMSIFAPKDMPRARKIIIERMSHPKYLAIK
                     DNGPAGMTYVPIDGDRAVFAWGNHLDQDIAPHLQLWRHHHVINNKKLVYDKDLNKIID
                     KTDDSGNRYITQATSLDKDFI"
BASE COUNT          109 a           66 c           60 g          137 t
ORIGIN      
        1 ctgataaaat ctttatctaa tgacgttgcc tgagtaatgt atctattacc agagtcatct
       61 gttttatcaa tgattttatt taaatcttta tcgtatacta actttttatt gttaataaca
      121 tggtgatgac gccacaattg aagatgtgga gcaatatctt gatctaaatg attaccccac
      181 gcaaaaacag ccctatcacc atcaatagga acgtaggtca tacctgctgg gccattgtct
      241 tttattgcga ggtatttagg atgagacatt ctttcaatga ttatttttct tgctctcggc
      301 atatctttag gagcaaaaat actcatatct atttgacaat aatcccattc ggtttctgat
      361 tgttggatat ta
//
LOCUS       DAJVQR010000361          372 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVQR010000361 DAJVQR010000000
VERSION     DAJVQR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..311)
                     /locus_tag="OQM52_004361"
     CDS             complement(<1..311)
                     /locus_tag="OQM52_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1565928.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJVQR010000362          367 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVQR010000362 DAJVQR010000000
VERSION     DAJVQR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>367)
                     /locus_tag="OQM52_004362"
     CDS             complement(<1..>367)
                     /locus_tag="OQM52_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCS1565929.1"
                     /translation="FGLRHRFRILNNKIINRVTGSEFVFYGLWRNIEEIKSLEGIDML
                     WLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVTDFVWRNFVVDPPEGTLIRKIN
                     YDENPFLSDTMLKVIDAARR"
BASE COUNT           99 a           92 c           83 g           93 t
ORIGIN      
        1 gcgtcgcgcc gcgtcgataa ccttaagcat ggtgtcagac agaaacggat tttcgtcata
       61 gttaattttg cggatgagag tgccttcggg cggatcaaca acgaagttgc gccagacgaa
      121 atcagtaaca agtccggggt tgaatatgaa ccagcattcc gaaccctctt tacggatcgt
      181 tggctccaga attttccact ggtattccgt cagtgcgtgg gcttcttcca accacaacat
      241 atcgatcccc tccagtgact taatttcttc gatgttgcgc cataatccat aaaaaacaaa
      301 ttccgagcca gtaacccggt taatgatttt gttgttcaga atacggaaac ggtgccgcag
      361 accaaac
//
LOCUS       DAJVQR010000363          365 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVQR010000363 DAJVQR010000000
VERSION     DAJVQR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          100 a           86 c          112 g           67 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gggtgaaact ctgcggcctt tttcgtaaga aaaatgcaac gattgcacgc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagacg tgacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg gcaagccgga aaggttccgg tgaggcgcaa tgttgcgggg
      241 gctttatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa catcacgggg gctaatcttg aacccaaaca actcaagaat agccgcgaaa
      361 cgttg
//
LOCUS       DAJVQR010000364          354 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVQR010000364 DAJVQR010000000
VERSION     DAJVQR010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>354
                     /locus_tag="OQM52_004363"
     CDS             <1..>354
                     /locus_tag="OQM52_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1565930.1"
                     /translation="VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLE
                     PKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSAL
                     GYRSPREYLRQRACN"
BASE COUNT           90 a           85 c          110 g           69 t
ORIGIN      
        1 acgtcatgct gggagcggtg gaacgccgct tcggcaacga tcttccgtcg tctccagtgg
       61 agtggctgac ggataatggt tcatgctacc gggctaatga aacacgccag ttcgcccgga
      121 tgttgggact tgaaccgaag aacacggcgg tgcggagtcc ggagagtaac ggaatagcag
      181 agagcttcgt gaaaacgata aagcgtgact acatcagtat catgcccaaa ccagacgggt
      241 taacggcagc aaagaacctt gcagaggcgt tcgagcatta taacgaatgg catccgcata
      301 gtgcgctggg ttatcgctcg ccacgggaat atctgcggca gcgggcttgt aatg
//
LOCUS       DAJVQR010000365          346 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVQR010000365 DAJVQR010000000
VERSION     DAJVQR010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(78..275)
                     /locus_tag="OQM52_004364"
     CDS             complement(78..275)
                     /locus_tag="OQM52_004364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565931.1"
                     /translation="MRNKVLEAWFYIVVAMTFTGYSFYLFFETTDISRYGVIGVIFNL
                     VSLKLLYEAYKINKEIKRKGF"
BASE COUNT          119 a           69 c           39 g          119 t
ORIGIN      
        1 aaaaagacta ataacaaccg tcaacaccag agaaaaccaa gcccctgtta aggggctttt
       61 atttggatta accattctta aaaacctttt ctttttattt ctttgtttat tttgtaagcc
      121 tcatataaca gctttaatga aaccagatta aaaataacac ctatcacacc atatcttgat
      181 atgtctgttg tctcgaaaaa cagataaaat gagtatcctg taaatgtcat tgctacaact
      241 atataaaacc atgcttctaa aactttattt ctcataatac acctctttat ttatcgtaaa
      301 agtaaactgt gattggtttg tctgaatatc catcttcacc attcgc
//
LOCUS       DAJVQR010000366          345 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVQR010000366 DAJVQR010000000
VERSION     DAJVQR010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="OQM52_004365"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="OQM52_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCS1565932.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAJVQR010000367          344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVQR010000367 DAJVQR010000000
VERSION     DAJVQR010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OQM52_004366"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OQM52_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1565933.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJVQR010000368          344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVQR010000368 DAJVQR010000000
VERSION     DAJVQR010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..311
                     /locus_tag="OQM52_004367"
     CDS             <1..311
                     /locus_tag="OQM52_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCS1565934.1"
                     /translation="AFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGA
                     TLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA
                     "
BASE COUNT           68 a           97 c           77 g          102 t
ORIGIN      
        1 tggcttttat ggataacgcc cacgttcagg tcacctttct ggtggagaaa ctctcccccg
       61 catggcaacg acgagtcgct ctggttaccc attccttaat cttatttatt tgtggcgcac
      121 tggcctgggg cgcaacgcta aaaacaattc aggactggag cgattattca ccgatcctcg
      181 gtttgcccat cggcctgatg tatgccgctt gtctgcccac cagcctcgtt atcgcattct
      241 ttgaactgcg tcatttatat caactcatca cgcgtagcaa ttctctaacg tcgccaccgc
      301 aaggagctta atcatggctg tgctgatttt tctgggctgt ctgt
//
LOCUS       DAJVQR010000369          344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVQR010000369 DAJVQR010000000
VERSION     DAJVQR010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          132 a           50 c           48 g          114 t
ORIGIN      
        1 tttatatcca tcttcttttg aatggagcag atccccaaaa gaaaaatagc aaatacaaat
       61 ccacgtatga aataatcatc aaagatgaaa atgaaaaatt tctttcctat atcatgcggt
      121 atgagcaaag tctgattaat gaaatacaca aaagaaaagg cactcattaa gagtgctttt
      181 tctaaaagtg atacttatat gatgtcaaaa cttacaataa atagaaattg ggtattaata
      241 tttacgtgtt aattaagtaa tcaattatct ttcttccgtc aatagcagtt gaaaaatatt
      301 ttctcttttc ttcaaaaaag tgttgatcgt cataagcgtt ctgt
//
LOCUS       DAJVQR010000370          343 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVQR010000370 DAJVQR010000000
VERSION     DAJVQR010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(141..>343)
                     /locus_tag="OQM52_004368"
     CDS             complement(141..>343)
                     /locus_tag="OQM52_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1565935.1"
                     /translation="ISVLRQRYEGRGMTKCKMAELLNDAHPKWSLRTCERRIEHWLKV
                     AEFILYKPMVMAFGIEKKVIAF"
BASE COUNT           92 a           90 c           60 g          101 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tca
//
LOCUS       DAJVQR010000371          343 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVQR010000371 DAJVQR010000000
VERSION     DAJVQR010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            1..343
                     /locus_tag="OQM52_004369"
                     /pseudo
     CDS             1..343
                     /locus_tag="OQM52_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a           73 c           83 g           78 t
ORIGIN      
        1 tatgaagagc gcggagaagc tggtttacgc gcgctcaaga ttggcaccaa aagaaacatt
       61 gcaatatcag ttgatccaga aaaagcggca tcagcattgg agctgtcaaa agaccgacgc
      121 attgaggatc ttgaaaggca agttcgattt cttgaaacgc ggcttgtgta tctaaaaaag
      181 ctgaaagcct tagctcatcc cacgaaaaag tgaaagtact caacgagcta aggcagtttt
      241 atcctcttga tgagcttctc agggctgcgg agataccgcg cagtacgttt tattaccatc
      301 taaaggctct cagcaagcct gacaagtatg cggacgttaa aaa
//
LOCUS       DAJVQR010000372          342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVQR010000372 DAJVQR010000000
VERSION     DAJVQR010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.392"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(109..303)
                     /locus_tag="OQM52_004370"
     CDS             complement(109..303)
                     /locus_tag="OQM52_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCS1565936.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT           89 a           87 c           65 g          101 t
ORIGIN      
        1 ttcaagatag gcgcgccgtg tattttaatt cttttaagac tcttcaaaaa tgtcatcgta
       61 gcctttaaaa acgaatttta aaggcaccct aaaggcaggt caatgaagtt aacccgcctt
      121 cgcgtttaca ctctctgaga gaatactagc ttcaatctct tccaaaatct ctctgacttg
      181 ctcgtcgggc atatcaacga gcatcaacgc tgccaggcgt ttacggccct ctagttgcag
      241 tagctcttga gtttccttat ctaacaagcg tagcacagtc tcaacacctt tccttgcgag
      301 catgtttgta agggcttctc gttcctcact ggtaagaaac ga
//
LOCUS       DAJVQR010000373          340 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVQR010000373 DAJVQR010000000
VERSION     DAJVQR010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>340)
                     /locus_tag="OQM52_004371"
     CDS             complement(<1..>340)
                     /locus_tag="OQM52_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1565937.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DAJVQR010000374          335 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVQR010000374 DAJVQR010000000
VERSION     DAJVQR010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.394"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..233
                     /locus_tag="OQM52_004372"
     CDS             <1..233
                     /locus_tag="OQM52_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCS1565938.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT           82 a           72 c           84 g           97 t
ORIGIN      
        1 tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat atcgtgtcac
       61 ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga tataaggcgc
      121 ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata atggatgctg
      181 aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa taaggagtga
      241 gtcgtcagag ctgcgatata tccccttcct ccatgggctg tctcttatac acaaatcccc
      301 acccggggat ttgtgatgtc agatgtcccc tgtaa
//
LOCUS       DAJVQR010000375          332 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVQR010000375 DAJVQR010000000
VERSION     DAJVQR010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            51..>332
                     /locus_tag="OQM52_004373"
     CDS             51..>332
                     /locus_tag="OQM52_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1565939.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAJVQR010000376          330 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVQR010000376 DAJVQR010000000
VERSION     DAJVQR010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.396"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>330
                     /locus_tag="OQM52_004374"
     CDS             <1..>330
                     /locus_tag="OQM52_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCS1565940.1"
                     /translation="PAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAIVVASVK
                     EAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDE
                     EKTQPSG"
BASE COUNT           91 a           89 c           80 g           70 t
ORIGIN      
        1 atccggcaca cgctaaacgt gtcgaggaaa ttatcgctga aaataaaccg cccttttctg
       61 ttttccgcga caaattcatc accatgcctg gcgggatgga ttattcccgc gccatcgtgg
      121 ttgcgtcagt gaaagaagca ccgattggta tcgaggtcat ccccgctcac gtcactgaat
      181 atctgaacaa ggtactgact gaaaccgatc atgccaaccc tgatccggaa atcgtggata
      241 ttgcctgcgg tcgctcctct gccccgatgc cgcaacgtgt aacagaagaa ggaaaacagg
      301 atgatgaaga aaaaacgcag ccatctggcg
//
LOCUS       DAJVQR010000377          328 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVQR010000377 DAJVQR010000000
VERSION     DAJVQR010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.397"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            56..>328
                     /locus_tag="OQM52_004375"
     CDS             56..>328
                     /locus_tag="OQM52_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565941.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEW"
BASE COUNT          107 a           70 c           82 g           69 t
ORIGIN      
        1 ggccacatcc ctccggcaga agcagaaaaa gcttattatg cttccatcgg aaacgatgat
       61 ctgtcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg ttcagaaagt
      121 aaatgaaaca gatgctgaat acctggtgcg taaattcgat gagcttgaag ctaaatgtgc
      181 agcacaggaa aacaaagtaa taccagtgtc aactgaactg ccaccagcaa atgaaagtgt
      241 tttgttattc gatgctaacg gagaaggctg gctaattggc tggcgttctc tctggtacac
      301 ctggggacaa aaagaaaccg gagaatgg
//
LOCUS       DAJVQR010000378          325 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVQR010000378 DAJVQR010000000
VERSION     DAJVQR010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.398"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT           90 a           73 c           82 g           80 t
ORIGIN      
        1 gcatctggct ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct
       61 acaaagcaga aggccgtgca ggatattcac cgggtcttgg cggggtataa ataccatgcg
      121 cattgactga attcgcaaac aaaaacagac gcgcgatacc cggatagtcg gtctgcatag
      181 tcagatatct ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg
      241 tccgggagtt gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag
      301 agcatatatt tatgaagccc ttcgt
//
LOCUS       DAJVQR010000379          325 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVQR010000379 DAJVQR010000000
VERSION     DAJVQR010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.399"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT           85 a           48 c           95 g           97 t
ORIGIN      
        1 gggaaaagct tttttcatgc actatttttg caaatatttc tatatgggaa gtggttttgt
       61 tacagattaa atttataaag aaatgcattt ggggtgttgg gttgaagagt agctggtaga
      121 tgttattgtg ccagcagggg caggggaatg tgccgtatcg atatgctttg gcaattgcgt
      181 gtgccgcaga tggagatgaa atgccaataa aaaagcctga gttcgatgag ggacgaactc
      241 aggctggtgt tatgttttac agtaacacgc ctcggcgcgt tggctgatta gaactggtag
      301 acgataccaa ctgccagttg gtcgt
//
LOCUS       DAJVQR010000380          322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVQR010000380 DAJVQR010000000
VERSION     DAJVQR010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.400"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT           70 a           78 c           84 g           90 t
ORIGIN      
        1 atccttttca atgaatcgta atctcttgct ccgtaaacga aaaaaccgcc tggggaggcg
       61 gtttgttcga aggttaagtc agttggggaa ctgcttaacc cggtaacagg gttcacgaga
      121 ccgccattac caaaactgtt ctttcagtgt agccgcagtc aggcctctac ttcaagacct
      181 cttaatctat cttctcgtgc actcctgtta ctaatggctg ctgccagtgg cgttttgtcg
      241 tgtctttccg ggttggactc aagttgatag ttaccggaca aggcgcagcg gtcggactga
      301 acggggggtt cgtgcataca gt
//
LOCUS       DAJVQR010000381          319 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVQR010000381 DAJVQR010000000
VERSION     DAJVQR010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.401"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            92..>319
                     /locus_tag="OQM52_004376"
     CDS             92..>319
                     /locus_tag="OQM52_004376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565942.1"
                     /translation="MNNFVDKYLNPEAIGAFSGIMGLFLHFLLVLGGILAAYVLISGT
                     MVTAHDGEMLGKKWSSMWLPIRTALGTAMLLP"
BASE COUNT           86 a           54 c           69 g          110 t
ORIGIN      
        1 aatattgctt ataatggcaa tgctattata tgcgacaaat gcgagtgccg acactatgga
       61 ttggttaact ccactgtcaa cagattggtt tatgaacaac tttgtagaca aataccttaa
      121 tccagaagct ataggcgctt tctcaggaat aatggggttg tttcttcatt ttcttttagt
      181 tttaggtgga atattagccg cttatgtttt gattagcgga actatggtca cggctcatga
      241 tggtgaaatg ttaggtaaaa aatggtcttc tatgtggctt cctatcagaa cagctttagg
      301 aacggctatg cttcttcct
//
LOCUS       DAJVQR010000382          317 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVQR010000382 DAJVQR010000000
VERSION     DAJVQR010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.403"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            79..>317
                     /locus_tag="OQM52_004377"
     CDS             79..>317
                     /locus_tag="OQM52_004377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565943.1"
                     /translation="MMTLLSTFNYIPAFIVGLVMMFLSVKVVLLPMADLITKIRDKTT
                     DVAIYPLSVFMGIPAIAVFFVAVSFTVSMFAYMVG"
BASE COUNT           97 a           47 c           49 g          124 t
ORIGIN      
        1 aatacttaaa aaataatttg aattccataa aactgtacga tattatttaa tcaacaaaca
       61 acgagagagg attttaaaat gatgacatta cttagcactt tcaactacat tccagctttt
      121 attgttggtt tagtaatgat gtttttatct gttaaagtag ttcttttacc aatggctgat
      181 ttaatcacta aaatcagaga caaaactact gatgttgcta tttaccctct tagcgttttt
      241 atgggtattc cggctatcgc agtctttttt gtagctgtat cttttacagt aagtatgttt
      301 gcttacatgg ttggatt
//
LOCUS       DAJVQR010000383          309 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVQR010000383 DAJVQR010000000
VERSION     DAJVQR010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.404"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT          105 a           59 c           59 g           86 t
ORIGIN      
        1 ggcgggggag aaatccctcg ccacctctca tgtgtcaggc atcctcaacg cacccgcact
       61 aaacccgctt cggcgggttt tttgttgcgt gctgaatacg cagggtgaaa aataaccata
      121 tatttgatta tatacacaaa aaaaaataaa agtcattgta cttgcacatt aaacaatcaa
      181 atatacggcg tgaaataaat atttatcaga ttaatatttt tgtctctatg tggatataac
      241 cgtttgtact tataaacctg ggggcatcgt ggaaaaaata aagaaactat ttagtagcaa
      301 atacgcagt
//
LOCUS       DAJVQR010000384          305 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVQR010000384 DAJVQR010000000
VERSION     DAJVQR010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.405"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>305)
                     /locus_tag="OQM52_004378"
     rRNA            complement(<1..>305)
                     /locus_tag="OQM52_004378"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           55 a          104 c           68 g           78 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaa
//
LOCUS       DAJVQR010000385          304 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVQR010000385 DAJVQR010000000
VERSION     DAJVQR010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.406"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            8..>304
                     /locus_tag="OQM52_004379"
     CDS             8..>304
                     /locus_tag="OQM52_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HCS1565944.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVH"
BASE COUNT           95 a           77 c           64 g           68 t
ORIGIN      
        1 aaaaacaatg agcacaaaac cactcttcct gttacggaaa gcgaaaaaat catccggtga
       61 acctgacgtc gtcctgtggg caagcgacga ttttgaatcg accagcacca ctctggacta
      121 tctgctcgtt aagtcaggta aaaaactgag caactatttt aaagctgttg ccacaaattt
      181 tcctgtcgtt aatgaccttc cccctgaagg tgagatcgat tttacctgga gtgaacgcta
      241 tcaactcagc aaagactcca tgacatggga actaaaaccg ggagcagcgc cagacgacgt
      301 tcac
//
LOCUS       DAJVQR010000386          304 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVQR010000386 DAJVQR010000000
VERSION     DAJVQR010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.407"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>304
                     /locus_tag="OQM52_004380"
     CDS             <1..>304
                     /locus_tag="OQM52_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565945.1"
                     /translation="TNSSSSGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFK
                     SLNLSLEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNF"
BASE COUNT           96 a           62 c           46 g          100 t
ORIGIN      
        1 taacaaactc atcttcttct ggcataagtg aaaaacattt agatcactgt gctaatactg
       61 tgaaaaactt tttaaggaag tctatagcag cgcaaagtta tagcaaaatg ttttctcaag
      121 ggaccagttt caaatcacta aatctttctc ttgaagcgcc atctggagca cggtcatcat
      181 ttcggtcact tgagcatctc gataaggtgt ccaggcacta tatctctgag ataatacaaa
      241 aagttcatcc tctttcttca gacgaacgtc acttactttc tattattatt aattctaatt
      301 ttaa
//
LOCUS       DAJVQR010000387          302 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVQR010000387 DAJVQR010000000
VERSION     DAJVQR010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.408"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..>302)
                     /locus_tag="OQM52_004381"
     CDS             complement(<1..>302)
                     /locus_tag="OQM52_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1565946.1"
                     /translation="WEKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTAR
                     HVVITGGEPCIHDLLPLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRG"
BASE COUNT           67 a           94 c           68 g           73 t
ORIGIN      
        1 cgccgcgcat gttcagcttt ggcgatacgg taacccaggt attcggtgtg cagcgtactt
       61 catgagttcc actggtttcg atctggcagc taaaaccgtt cttttcgagc aggtcagtca
      121 gtggcagcaa atcatgaatg caaggctcac cacccgtaat caccacatgc cgcgcggtat
      181 atccctgacg accaatgaca gccagcaagt cttcactgct cgccgccccc cacttatcac
      241 tctctttggt cttcgccaga atgctgaaaa gggagacttc ccgatcctca agcttttccc
      301 ag
//
LOCUS       DAJVQR010000388          289 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVQR010000388 DAJVQR010000000
VERSION     DAJVQR010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.410"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT           86 a           74 c           54 g           75 t
ORIGIN      
        1 tcacaaaatt cactttaaaa agggcggaca tcagccagca attaaaccga tgccgccaac
       61 tggtacttca cacagcaatg tcgttattta caaccggaag cgcactccca ccatttaaat
      121 ttcacagaca agaccgactc tttatggata ccggaaatgc gccttcgtgt tgtgcccggt
      181 tttatttcac cacttccggg ctttggtggt atctttactg aagttctcac acaaccagta
      241 aggaaatgaa tatgccaacg tatctcgcca gagtagaact atataatgc
//
LOCUS       DAJVQR010000389          287 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVQR010000389 DAJVQR010000000
VERSION     DAJVQR010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.411"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
BASE COUNT           69 a           58 c           73 g           87 t
ORIGIN      
        1 tgttagtctt tgcatgagca agcggactga cacatttctc tttgttctca ttcagaaaat
       61 ctcatcagtc ggcgttcaga ccggtgttgt gctttatgga tacgttacgc tcctgatgac
      121 gtcatttgac gttcaacagc atcacggggc cgcacgacat ttcacgtcag ttagtgctat
      181 agctcaggaa caaattttcc caaattggga tatgctcgca aattgctggt gatgtgggga
      241 gaatctggtt gagttcggta gaattgattt ggaggcagaa cgcttaa
//
LOCUS       DAJVQR010000390          286 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVQR010000390 DAJVQR010000000
VERSION     DAJVQR010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.denovo.412"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>286
                     /locus_tag="OQM52_004382"
     CDS             <1..>286
                     /locus_tag="OQM52_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1565947.1"
                     /translation="LTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELDLE
                     ASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDS"
BASE COUNT           81 a           56 c           72 g           77 t
ORIGIN      
        1 ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc ttatgcccgt
       61 gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg tcagtttgaa
      121 ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc atatgctggt
      181 ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt cgcgtatgat
      241 gcagaagcat ttactgatat gtttgttgag tggggcggtg actcat
//
LOCUS       DAJVQR010000391         2590 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.223, whole genome shotgun sequence.
ACCESSION   DAJVQR010000391 DAJVQR010000000
VERSION     DAJVQR010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.223"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..340
                     /gene="frdC"
                     /locus_tag="OQM52_004383"
     CDS             <1..340
                     /gene="frdC"
                     /locus_tag="OQM52_004383"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1565948.1"
                     /translation="LPFYRFYMLREGTAVPAVWFSIELIFALFALKNGPEAWAGFVDF
                     LQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDEKMGPEPIIKSLWAVTVVAT
                     IVILFVALYW"
     gene            351..710
                     /gene="frdD"
                     /locus_tag="OQM52_004384"
     CDS             351..710
                     /gene="frdD"
                     /locus_tag="OQM52_004384"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1565949.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            773..1906
                     /locus_tag="OQM52_004385"
     CDS             773..1906
                     /locus_tag="OQM52_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1565950.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1995..2528
                     /gene="blc"
                     /locus_tag="OQM52_004386"
     CDS             1995..2528
                     /gene="blc"
                     /locus_tag="OQM52_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1565951.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
BASE COUNT          618 a          660 c          669 g          643 t
ORIGIN      
        1 attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt
       61 cagcattgaa ctgattttcg cgctgtttgc cctgaaaaat ggcccggaag cctgggcggg
      121 attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca ctctgacggc
      181 agctctgctg cacaccaaaa cctggtttga actggcaccg aaagcggcca atatcattgt
      241 aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt
      301 tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc
      361 caaatccaaa gcgttctgac gaaccggtat tctggggcct cttcggggcc ggtggtatgt
      421 ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg tattctgctg ccactggggc
      481 tgtttccggg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg
      541 gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgtatgc
      601 accacgcgat gcacgatctg aaaatccacg tgcctgcggg caaatgggtt ttctacggtc
      661 tggctgctat cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa cgcatcgcca
      721 atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa
      781 aacgacgctc tgcgccttat taattaccgc ctcttactcc acatttgctg cccctcaaca
      841 aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg
      901 tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga
      961 catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa
     1021 aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga
     1081 tcccgcaaca aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt
     1141 acatcttgcg acctacaccg ctggcggcct gccattgcag gtgccggatg aagtgaaatc
     1201 ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca
     1261 acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg
     1321 tttgagtttt gagcaggcga tgcaaactcg tgtcttccag ccactcaaac tcaaccatac
     1381 gtggattaat gtacctcccc cagaagaaaa gaattacgcc tggggatatc gcgaaggtaa
     1441 ggcagtgcat gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat
     1501 tgaagatatg gcccgctggg tgcaaagcaa tttacccccc cttgatatca atgagaaaac
     1561 gcttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca
     1621 gggcctgggc tgggaaatgc tggactggcc ggtcaatcct gacagcatca ttaacggcag
     1681 tgacaataaa attgcactgg cagcacgccc ggtaaaagcg attaagcccc caactcctgc
     1741 agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc
     1801 gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc
     1861 agcgagagtc gccgccgcct gtcagattct caacgctcta cagtaaaatt tcatcgggtc
     1921 cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca
     1981 gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg
     2041 tcgttgcctg tagttttcct acgccgccgc gtggcgtgac cgtagtgaat aatttcgacg
     2101 ccaaacgtta tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg
     2161 gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg aatgtcatta
     2221 ataaaggcta taaccctgac agaggaatgt ggcagcagag tgaagggaaa gcgtacttta
     2281 ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt
     2341 ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg
     2401 actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc
     2461 tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg
     2521 gtagttagtg agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg
     2581 ccagacgcat
//
LOCUS       DAJVQR010000392         2276 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.231, whole genome shotgun sequence.
ACCESSION   DAJVQR010000392 DAJVQR010000000
VERSION     DAJVQR010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.231"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            18..257
                     /locus_tag="OQM52_004387"
     CDS             18..257
                     /locus_tag="OQM52_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1565952.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(445..1422)
                     /gene="intI2"
                     /locus_tag="OQM52_004388"
                     /pseudo
     CDS             complement(445..1422)
                     /gene="intI2"
                     /locus_tag="OQM52_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1754..2227
                     /locus_tag="OQM52_004389"
     CDS             1754..2227
                     /locus_tag="OQM52_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1565953.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
BASE COUNT          665 a          467 c          492 g          652 t
ORIGIN      
        1 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
       61 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      121 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      181 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      241 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      301 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      361 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      421 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
      481 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
      541 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
      601 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
      661 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
      721 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
      781 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
      841 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
      901 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
      961 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1021 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1081 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1141 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1201 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1261 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1321 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1381 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1441 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     1501 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     1561 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     1621 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     1681 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     1741 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     1801 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     1861 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     1921 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     1981 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2041 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2101 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2161 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2221 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaa
//
LOCUS       DAJVQR010000393         2079 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.241, whole genome shotgun sequence.
ACCESSION   DAJVQR010000393 DAJVQR010000000
VERSION     DAJVQR010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.241"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..222)
                     /gene="tetR(B)"
                     /locus_tag="OQM52_004390"
     CDS             complement(<1..222)
                     /gene="tetR(B)"
                     /locus_tag="OQM52_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1565954.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGES"
     gene            304..1509
                     /gene="tet(B)"
                     /locus_tag="OQM52_004391"
     CDS             304..1509
                     /gene="tet(B)"
                     /locus_tag="OQM52_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1565955.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1622..>2079)
                     /locus_tag="OQM52_004392"
     CDS             complement(1622..>2079)
                     /locus_tag="OQM52_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1565956.1"
                     /translation="KGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALE
                     SIFEHYLDYIIDNNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLK
                     AYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          533 a          386 c          442 g          718 t
ORIGIN      
        1 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
       61 ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc
      121 tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
      181 taatgcgctg ttaatcactt tacttttatc taatctagac atcattaatt cctaattttt
      241 gttgacactc tatcattgat agagttattt taccactccc tatcagtgat agagaaaagt
      301 gaaatgaata gttcgacaaa gatcgcattg gtaattacgt tactcgatgc catggggatt
      361 ggccttatca tgccagtctt gccaacgtta ttacgtgaat ttattgcttc ggaagatatc
      421 gctaaccact ttggcgtatt gcttgcactt tatgcgttaa tgcaggttat ctttgctcct
      481 tggcttggaa aaatgtctga ccgatttggt cggcgcccag tgctgttgtt gtcattaata
      541 ggcgcatcgc tggattactt attgctggct ttttcaagtg cgctttggat gctgtattta
      601 ggccgtttgc tttcagggat cacaggagct actggggctg tcgcggcatc ggtcattgcc
      661 gataccacct cagcttctca acgcgtgaag tggttcggtt ggttaggggc aagttttggg
      721 cttggtttaa tagcggggcc tattattggt ggttttgcag gagagatttc accgcatagt
      781 ccctttttta tcgctgcgtt gctaaatatt gtcgctttcc ttgtggttat gttttggttc
      841 cgtgaaacca aaaatacacg tgataataca gataccgaag taggggttga gacgcaatcg
      901 aattcggtat acatcacttt atttaaaacg atgcccattt tgttgattat ttatttttca
      961 gcgcaattga taggccaaat tcccgcaacg gtgtgggtgc tatttaccga aaatcgtttt
     1021 ggatggaata gcatgatggt tggcttttca ttagcgggtc ttggtctttt acactcagta
     1081 ttccaagcct ttgtggcagg aagaatagcc actaaatggg gcgaaaaaac ggcagtactg
     1141 ctcggattta ttgcagatag tagtgcattt gcctttttag cgtttatatc tgaaggttgg
     1201 ttagttttcc ctgttttaat tttattggct ggtggtggga tcgctttacc tgcattacag
     1261 ggagtgatgt ctatccaaac aaagagtcat cagcaaggtg ctttacaggg attattggtg
     1321 agccttacca atgcaaccgg tgttattggc ccattactgt ttgctgttat ttataatcat
     1381 tcactaccaa tttgggatgg ctggatttgg attattggtt tagcgtttta ctgtattatt
     1441 atcctgctat cgatgacctt catgttaacc cctcaagctc aggggagtaa acaggagaca
     1501 agtgcttagt tatttcgtca ccaaatgatg ttattccgcg aaatataatg accctcttga
     1561 taacccaaga gggcattttt tacgataaag aagatttagc ttcaaataaa acctatctat
     1621 tttatttatc tttcaagctc aataaaaagc cgcggtaaat agcaataaat tggccttttt
     1681 tatcggcaag ctcttttagg tttttcgcat gtattgcgat atgcataaac cagccattga
     1741 gtaagttttt aagcacatca tcatcataag ctttaagttg gttctcttgg atcaatttgc
     1801 tgacaatggc gtttacctta ccagtaatgt attcaaggct aattttttca agttcattcc
     1861 aaccaatgat aggcatcact tcttggatag ggataaggtt tttattatta tcaataatat
     1921 aatcaagata atgttcaaat atactttcta aggcagacca accatttgtt aaatcagttt
     1981 ttgttgtgat gtaggcatca atcataatta attgctgctt ataacaggca ctgagtaatt
     2041 gttttttatt tttaaagtga tgataaaagg cacctttgg
//
LOCUS       DAJVQR010000394         2029 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.244, whole genome shotgun sequence.
ACCESSION   DAJVQR010000394 DAJVQR010000000
VERSION     DAJVQR010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.244"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..1194)
                     /gene="pepD"
                     /locus_tag="OQM52_004393"
     CDS             complement(<1..1194)
                     /gene="pepD"
                     /locus_tag="OQM52_004393"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HCS1565957.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPG"
     gene            1456..1914
                     /gene="gpt"
                     /locus_tag="OQM52_004394"
     CDS             1456..1914
                     /gene="gpt"
                     /locus_tag="OQM52_004394"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1565958.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT          485 a          521 c          509 g          514 t
ORIGIN      
        1 gccaggatat gcgcctttcg cttcggtttt cgcgccagcc agtttaccca gcgaatccag
       61 catgctcacc acgtagtctt taccgctgtc gattagtgaa cggatcaggc agtgaatttc
      121 tacgttattg tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt
      181 tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt
      241 atcgcgagat ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc
      301 cagatttttc tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag
      361 agatttcagt gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg
      421 gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg cgcaggtcca gttcttctgc
      481 atgacccgcc aggaagcgca ccagcagttt gttggcatta cccaggccaa cgtggatttc
      541 accgccggag tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc
      601 tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc
      661 catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca
      721 gttgctctgt aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac
      781 ttccagcggg ccgtgaacca cgttttcgtc agccagaacc gccagtgcag aggccatacc
      841 aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata
      901 aggctggata ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat
      961 atcgaggtgg gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttg
     1021 acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca
     1081 accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat
     1141 tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag
     1201 tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg
     1261 tcaacacaag acaggcttgc gagatatgta ttgagaatac cactttatcc cgcgtcaggg
     1321 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     1381 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     1441 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     1501 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     1561 agccgtggcg gtctggtacc gggggcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     1621 gataccgttt gtatctccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
     1681 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
     1741 actgctgttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
     1801 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc
     1861 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     1921 caacgcctgg cattgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc
     1981 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaa
//
LOCUS       DAJVQR010000395         2029 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.245, whole genome shotgun sequence.
ACCESSION   DAJVQR010000395 DAJVQR010000000
VERSION     DAJVQR010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.245"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..1194)
                     /gene="pepD"
                     /locus_tag="OQM52_004395"
     CDS             complement(<1..1194)
                     /gene="pepD"
                     /locus_tag="OQM52_004395"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HCS1565959.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPG"
     gene            1456..1914
                     /gene="gpt"
                     /locus_tag="OQM52_004396"
     CDS             1456..1914
                     /gene="gpt"
                     /locus_tag="OQM52_004396"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1565960.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT          485 a          520 c          509 g          515 t
ORIGIN      
        1 gccaggatat gcgcctttcg cttcggtttt cgcgccagcc agtttaccca gcgaatccag
       61 catgctcacc acgtagtctt taccgctgtc gattagtgaa cggatcaggc agtgaatttc
      121 tacgttattg tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt
      181 tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt
      241 atcgcgagat ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc
      301 cagatttttc tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag
      361 agatttcagt gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg
      421 gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg cgcaggtcca gttcttctgc
      481 atgacccgcc aggaagcgca ccagcagttt gttggcatta cccaggccaa cgtggatttc
      541 accgccggag tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc
      601 tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc
      661 catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca
      721 gttgctctgt aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac
      781 ttccagcggg ccgtgaacca cgttttcgtc agccagaacc gccagtgcag aggccatacc
      841 aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata
      901 aggctggata ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat
      961 atcgaggtgg gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttg
     1021 acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca
     1081 accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat
     1141 tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag
     1201 tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg
     1261 tcaacacaag acaggcttgc gagatatgta ttgagaatac cactttatcc cgcgtcaggg
     1321 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     1381 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     1441 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     1501 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     1561 agccgtggcg gtctggtacc gggggcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     1621 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
     1681 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
     1741 actgctgttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
     1801 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc
     1861 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     1921 caacgcctgg cattgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc
     1981 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaa
//
LOCUS       DAJVQR010000396         1384 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.266, whole genome shotgun sequence.
ACCESSION   DAJVQR010000396 DAJVQR010000000
VERSION     DAJVQR010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.266"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            complement(<1..181)
                     /locus_tag="OQM52_004397"
     CDS             complement(<1..181)
                     /locus_tag="OQM52_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCS1565961.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSE"
     gene            391..1221
                     /locus_tag="OQM52_004398"
     CDS             391..1221
                     /locus_tag="OQM52_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1565962.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          465 a          288 c          284 g          347 t
ORIGIN      
        1 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
       61 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      121 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      181 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      241 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      301 gcagtcgccc taaaacaaag ttgggcgaac ccggagcctc attaattgtt agccgttaaa
      361 attaagccct ttaccaaacc aatacttatt atgaaaaaca caatacatat caacttcgct
      421 atttttttaa taattgcaaa tattatctac agcagcgcca gtgcatcaac agatatctct
      481 actgttgcat ctccattatt tgaaggaact gaaggttgtt ttttacttta cgatgcatcc
      541 acaaacgctg aaattgctca attcaataaa gcaaagtgtg caacgcaaat ggcaccagat
      601 tcaactttca agatcgcatt atcacttatg gcatttgatg cggaaataat agatcagaaa
      661 accatattca aatgggataa aacccccaaa ggaatggaga tctggaacag caatcataca
      721 ccaaagacgt ggatgcaatt ttctgttgtt tgggtttcgc aagaaataac ccaaaaaatt
      781 ggattaaata aaatcaagaa ttatctcaaa gattttgatt atggaaatca agacttctct
      841 ggagataaag aaagaaacaa cggattaaca gaagcatggc tcgaaagtag cttaaaaatt
      901 tcaccagaag aacaaattca attcctgcgt aaaattatta atcacaatct cccagttaaa
      961 aactcagcca tagaaaacac catagagaac atgtatctac aagatctgga taatagtaca
     1021 aaactgtatg ggaaaactgg tgcaggattc acagcaaata gaaccttaca aaacggatgg
     1081 tttgaagggt ttattataag caaatcagga cataaatatg tttttgtgtc cgcacttaca
     1141 ggaaacttgg ggtcgaattt aacatcaagc ataaaagcca agaaaaatgc gatcaccatt
     1201 ctaaacacac taaatttata aaaaatctaa tggcaaaatc gcccaaccct tcaatcaagt
     1261 cgggacggcc aaaagcaagc ttttggctcc cctcgctggc gctcggcgcc ccttatttca
     1321 aacgttaaac atcatgaggg aagtggtgat cgccgaagta tcgactcaac tatcagaggt
     1381 agtt
//
LOCUS       DAJVQR010000397         1265 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.271, whole genome shotgun sequence.
ACCESSION   DAJVQR010000397 DAJVQR010000000
VERSION     DAJVQR010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.271"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..301
                     /locus_tag="OQM52_004399"
     CDS             <1..301
                     /locus_tag="OQM52_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCS1565963.1"
                     /translation="FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFT
                     ANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            414..1205
                     /gene="aadA1"
                     /locus_tag="OQM52_004400"
     CDS             414..1205
                     /gene="aadA1"
                     /locus_tag="OQM52_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1565964.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          360 a          301 c          311 g          293 t
ORIGIN      
        1 attcctgcgt aaaattatta atcacaatct cccagttaaa aactcagcca tagaaaacac
       61 catagagaac atgtatctac aagatctgga taatagtaca aaactgtatg ggaaaactgg
      121 tgcaggattc acagcaaata gaaccttaca aaacggatgg tttgaagggt ttattataag
      181 caaatcagga cataaatatg tttttgtgtc cgcacttaca ggaaacttgg ggtcgaattt
      241 aacatcaagc ataaaagcca agaaaaatgc gatcaccatt ctaaacacac taaatttata
      301 aaaaatctaa tggcaaaatc gcccaaccct tcaatcaagt cgggacggcc aaaagcaagc
      361 ttttggctcc cctcgctggc gctcggcgcc ccttatttca aacgttaaac atcatgaggg
      421 aagtggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      481 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      541 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
      601 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
      661 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
      721 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
      781 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
      841 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
      901 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
      961 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1021 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1081 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     1141 cagatcagtt ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca
     1201 aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     1261 ttaga
//
LOCUS       DAJVQR010000398         1262 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.272, whole genome shotgun sequence.
ACCESSION   DAJVQR010000398 DAJVQR010000000
VERSION     DAJVQR010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.272"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..301
                     /locus_tag="OQM52_004401"
     CDS             <1..301
                     /locus_tag="OQM52_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCS1565965.1"
                     /translation="FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFT
                     ANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            414..1202
                     /gene="aadA1"
                     /locus_tag="OQM52_004402"
     CDS             414..1202
                     /gene="aadA1"
                     /locus_tag="OQM52_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1565966.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          357 a          301 c          311 g          293 t
ORIGIN      
        1 attcctgcgt aaaattatta atcacaatct cccagttaaa aactcagcca tagaaaacac
       61 catagagaac atgtatctac aagatctgga taatagtaca aaactgtatg ggaaaactgg
      121 tgcaggattc acagcaaata gaaccttaca aaacggatgg tttgaagggt ttattataag
      181 caaatcagga cataaatatg tttttgtgtc cgcacttaca ggaaacttgg ggtcgaattt
      241 aacatcaagc ataaaagcca agaaaaatgc gatcaccatt ctaaacacac taaatttata
      301 aaaaatctaa tggcaaaatc gcccaaccct tcaatcaagt cgggacggcc aaaagcaagc
      361 ttttggctcc cctcgctggc gctcggcgcc ccttatttca aacgttaaac atcatgaggg
      421 aagtggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      481 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      541 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
      601 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
      661 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
      721 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
      781 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
      841 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
      901 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
      961 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1021 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1081 tcatacttga agctaggcag gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     1261 ga
//
LOCUS       DAJVQR010000399         1262 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.273, whole genome shotgun sequence.
ACCESSION   DAJVQR010000399 DAJVQR010000000
VERSION     DAJVQR010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.273"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..301
                     /locus_tag="OQM52_004403"
     CDS             <1..301
                     /locus_tag="OQM52_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCS1565967.1"
                     /translation="FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFT
                     ANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            414..1202
                     /gene="aadA1"
                     /locus_tag="OQM52_004404"
     CDS             414..1202
                     /gene="aadA1"
                     /locus_tag="OQM52_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1565968.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          357 a          300 c          311 g          294 t
ORIGIN      
        1 attcctgcgt aaaattatta atcacaatct cccagttaaa aactcagcca tagaaaacac
       61 catagagaac atgtatctac aagatctgga taatagtaca aaactgtatg ggaaaactgg
      121 tgcaggattc acagcaaata gaaccttaca aaacggatgg tttgaagggt ttattataag
      181 caaatcagga cataaatatg tttttgtgtc cgcacttaca ggaaacttgg ggtcgaattt
      241 aacatcaagc ataaaagcca agaaaaatgc gatcaccatt ctaaacacac taaatttata
      301 aaaaatctaa tggcaaaatc gcccaaccct tcaatcaagt cgggacggcc aaaagcaagc
      361 ttttggctcc cctcgctggc gctcggcgcc ccttatttca aacgttaaac atcatgaggg
      421 aagtggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      481 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      541 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
      601 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
      661 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
      721 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
      781 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaatatagcg
      841 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
      901 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
      961 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1021 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1081 tcatacttga agctaggcag gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     1261 ga
//
LOCUS       DAJVQR010000400         1051 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.284, whole genome shotgun sequence.
ACCESSION   DAJVQR010000400 DAJVQR010000000
VERSION     DAJVQR010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.284"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..142
                     /locus_tag="OQM52_004405"
     CDS             <1..142
                     /locus_tag="OQM52_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat1"
                     /protein_id="HCS1565969.1"
                     /translation="VPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQD
                     DA"
     gene            200..991
                     /gene="aadA1"
                     /locus_tag="OQM52_004406"
     CDS             200..991
                     /gene="aadA1"
                     /locus_tag="OQM52_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1565970.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          260 a          264 c          286 g          241 t
ORIGIN      
        1 tgtacctgcc tgcaatttgt acgcaaaatg tggctttact ctcggcggca ttgacctgtt
       61 cacgtataaa actagacctc aagtctcgaa cgaaacagcg atgtactggt actggttctc
      121 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt
      181 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
      241 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
      301 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
      361 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
      421 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
      481 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
      541 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
      601 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
      661 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
      721 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
      781 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
      841 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
      901 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa
      961 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg
     1021 cttcgcggcg cggcttaact caagcgttag a
//
LOCUS       DAJVQR010000401         1048 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.285, whole genome shotgun sequence.
ACCESSION   DAJVQR010000401 DAJVQR010000000
VERSION     DAJVQR010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.285"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..142
                     /locus_tag="OQM52_004407"
     CDS             <1..142
                     /locus_tag="OQM52_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat1"
                     /protein_id="HCS1565971.1"
                     /translation="VPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQD
                     DA"
     gene            200..988
                     /gene="aadA1"
                     /locus_tag="OQM52_004408"
     CDS             200..988
                     /gene="aadA1"
                     /locus_tag="OQM52_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1565972.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          257 a          264 c          286 g          241 t
ORIGIN      
        1 tgtacctgcc tgcaatttgt acgcaaaatg tggctttact ctcggcggca ttgacctgtt
       61 cacgtataaa actagacctc aagtctcgaa cgaaacagcg atgtactggt actggttctc
      121 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt
      181 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
      241 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
      301 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
      361 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
      421 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
      481 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
      541 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
      601 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
      661 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
      721 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
      781 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
      841 gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca
      901 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg
      961 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt
     1021 cgcggcgcgg cttaactcaa gcgttaga
//
LOCUS       DAJVQR010000402         1048 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.286, whole genome shotgun sequence.
ACCESSION   DAJVQR010000402 DAJVQR010000000
VERSION     DAJVQR010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.286"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..142
                     /locus_tag="OQM52_004409"
     CDS             <1..142
                     /locus_tag="OQM52_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat1"
                     /protein_id="HCS1565973.1"
                     /translation="VPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQD
                     DA"
     gene            200..988
                     /gene="aadA1"
                     /locus_tag="OQM52_004410"
     CDS             200..988
                     /gene="aadA1"
                     /locus_tag="OQM52_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1565974.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREYSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          257 a          263 c          286 g          242 t
ORIGIN      
        1 tgtacctgcc tgcaatttgt acgcaaaatg tggctttact ctcggcggca ttgacctgtt
       61 cacgtataaa actagacctc aagtctcgaa cgaaacagcg atgtactggt actggttctc
      121 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt
      181 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
      241 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
      301 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
      361 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
      421 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
      481 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
      541 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
      601 gctgacaaaa gcaagagaat atagcgttgc cttggtaggt ccagcggcgg aggaactctt
      661 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
      721 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
      781 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
      841 gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca
      901 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg
      961 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt
     1021 cgcggcgcgg cttaactcaa gcgttaga
//
LOCUS       DAJVQR010000403          938 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.291, whole genome shotgun sequence.
ACCESSION   DAJVQR010000403 DAJVQR010000000
VERSION     DAJVQR010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.291"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            31..690
                     /locus_tag="OQM52_004411"
     CDS             31..690
                     /locus_tag="OQM52_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1565975.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          244 a          200 c          226 g          268 t
ORIGIN      
        1 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
       61 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      121 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      181 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      241 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      301 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
      361 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
      421 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
      481 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
      541 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
      601 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
      661 tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa
      721 acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa
      781 gcaaattcga cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt
      841 gctggtttac cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct
      901 gaggccagtt tgctcaggct ctccccgtgg aggtaata
//
LOCUS       DAJVQR010000404          718 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.312, whole genome shotgun sequence.
ACCESSION   DAJVQR010000404 DAJVQR010000000
VERSION     DAJVQR010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.312"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            86..610
                     /gene="sat2"
                     /locus_tag="OQM52_004412"
     CDS             86..610
                     /gene="sat2"
                     /locus_tag="OQM52_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1565976.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          194 a          177 c          180 g          167 t
ORIGIN      
        1 gcaacggatt cgcaaacctg tcacgccttt tgtaccaaaa gccgcgccag gtttgcgatc
       61 cgctgtgcca ggcgttaggc gtcatatgaa gatttcggtg atccctgagc aggtggcgga
      121 aacattggat gctgagaacc atttcattgt tcgtgaagtg ttcgatgtgc acctatccga
      181 ccaaggcttt gaactatcta ccagaagtgt gagcccctac cggaaggatt acatctcgga
      241 tgatgactct gatgaagact ctgcttgcta tggcgcattc atcgaccaag agcttgtcgg
      301 gaagattgaa ctcaactcaa catggaacga tctagcctct atcgaacaca ttgttgtgtc
      361 gcacacgcac cgaggcaaag gagtcgcgca cagtctcatc gaatttgcga aaaagtgggc
      421 actaagcaga cagctccttg gcatacgatt agagacacaa acgaacaatg tacctgcctg
      481 caatttgtac gcaaaatgtg gctttactct cggcggcatt gacctgttca cgtataaaac
      541 tagacctcaa gtctcgaacg aaacagcgat gtactggtac tggttctcgg gagcacagga
      601 tgacgcctaa caattcattc aagccgacac cgcttcgcgg cgcggcttaa ttcaggagtt
      661 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagtt
//
LOCUS       DAJVQR010000405          483 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.352, whole genome shotgun sequence.
ACCESSION   DAJVQR010000405 DAJVQR010000000
VERSION     DAJVQR010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.352"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..481
                     /locus_tag="OQM52_004413"
     CDS             <1..481
                     /locus_tag="OQM52_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1565977.1"
                     /translation="PSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRI
                     AQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
BASE COUNT           97 a          134 c          142 g          110 t
ORIGIN      
        1 gccttctgcc cttctcccga tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga
       61 tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg gcgattatag ccgatcaaat
      121 gatgagcaat gcctcggaac tgcgtgggct acatggcgat ctgcatcatg aaaacatcat
      181 gttctccagt cgcggctggc tggtgataga tcccgtcggt ctggtcggtg aagtgggctt
      241 tggcgccgcc aatatgttct acgatccggc tgacagagac gacctttgtc tcgatcctag
      301 acgcattgca cagatggcgg acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct
      361 gctcgaccag gcgtacgctt atgggtgcct ttccgcagct tggaacgcgg atggagaaga
      421 ggagcaacgc gatctagcta tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta
      481 gat
//
LOCUS       DAJVQR010000406          475 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 602536_I
            SAMEA8901066-rid15852423.guided.356, whole genome shotgun sequence.
ACCESSION   DAJVQR010000406 DAJVQR010000000
VERSION     DAJVQR010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901066
            Sequence Read Archive: ERR6004986
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477900.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477900.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,414
            CDSs (total)                      :: 4,344
            Genes (coding)                    :: 3,962
            CDSs (with protein)               :: 3,962
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 182 of 382
            Pseudo Genes (incomplete)         :: 100 of 382
            Pseudo Genes (internal stop)      :: 144 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901066-rid15852423.guided.356"
                     /serovar="S. flexneri 2a"
                     /isolate="602536_I"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2009-05-08"
     gene            <1..>475
                     /locus_tag="OQM52_004414"
     CDS             <1..>475
                     /locus_tag="OQM52_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HCS1565978.1"
                     /translation="DWLPVRGGESGDFVSRRGDGHAFAKIAPASRRGELAGERDRLIW
                     LKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSL
                     SVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRL"
BASE COUNT           85 a          123 c          157 g          110 t
ORIGIN      
        1 ctgactggtt gcctgtcaga ggcggagaat ctggtgattt tgtttctcga cgtggtgacg
       61 ggcatgcctt cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct ggagagcgtg
      121 accgcctcat ttggctcaaa ggtcgaggtg tggcttgccc cgaggtcatc aactggcagg
      181 aggaacagga gggtgcatgc ttggtgataa cggcaattcc gggagtaccg gcggctgatc
      241 tgtctggagc ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc
      301 acagcctatc ggttgatcaa tgtccgtttg agcgcaggct gtcgcgaatg ttcggacgcg
      361 ccgttgatgt ggtgtcccgc aatgccgtca atcccgactt cttaccggac gaggacaaga
      421 gtacgccgca gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg cggct
//
